<?xml version="1.0" encoding="UTF-8"?>
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<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJD6</accession>
    <name>A0A8J4PJD6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010144</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000944">
        <property type="protein sequence ID" value="KAF2068530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJD6"/>
    <dbReference type="OrthoDB" id="17470at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="146"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="512" mass="58022" checksum="AB2F53CE0C908A49" modified="2022-05-25" version="1">MGFEPIYKASENEFSASSFHSACDGKGATITLIETTDGCVFGGYNSQSWNNDSKWYGDDKCFIFTLVNKHGIKPTKYSPGQDTHYVASNFDSGPIFGGGFDIGFDSSDGSSYQSFPYTYVDTTGQGKTTLTPTKSFKIKTIEIYKFGNKQNENDRLKQENKAIKQHLEKLSEENDNHNNKMDLLLSKIENVNQENKNYSKMMDILLEKMDSLINVNDKLNTKIENVNQENKKNVEDYSKIIELLSDKVDSLAEVNESLNIKIKNVDQQISQQQSTLIEQHHKSLEKIDSLIKANESIHQQLKQLHNSLDFKHLEICDKISKTCKEIESCQQDLLFLIYESKSTIIENASFKIINDWIDDSKTMGFELLYKASENEFSASSFHLACDGKGATITLIETTDGYCDNSKWCGDNKCFIFTLVNKHGIKPTKYSPGTKNTNYVGCSKNAGPIFGNGHDIGVNIKKGISYQNFPSTYIDTTGKGRSTLNPTIEFIIKTIEIYNVDKYHQPKWMVWMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PK50</accession>
    <name>A0A8J4PK50_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TPR repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_010436</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SGT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001111">
        <property type="protein sequence ID" value="KAF2068238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PK50"/>
    <dbReference type="OrthoDB" id="1898560at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06466">
        <property type="entry name" value="p23_CS_SGT1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007699">
        <property type="entry name" value="SGS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044563">
        <property type="entry name" value="Sgt1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45862">
        <property type="entry name" value="PROTEIN SGT1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05002">
        <property type="entry name" value="SGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51048">
        <property type="entry name" value="SGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="68"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="CS" evidence="5">
        <location>
            <begin position="175"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="SGS" evidence="4">
        <location>
            <begin position="282"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="117"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="139"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="154"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2068238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="365" mass="40588" checksum="9C74110C1C0BE937" modified="2022-05-25" version="1">MEHLNQGNSFFVDEQYDEALEAYNKACEAMPTNAEAFVKRSQCNQKLSNHTEALSDINEAIKIDSNNSKSYLKKGQICFELEEYEVALKAFEKGSSLEPESNVYKTWIRKAKAEIEASQPVKSTPTPTPAPATATTTPTPAPTTTPKPTPTPTPATVQTTPAEPTKPQLPIPSSGSKVRHEWYQTATHVIITIFAKFVTAQNSKINVTSKSILVSFNMANGSEFIYEIDFFDPVTEAETLIQYYNTKVEIKLKKSRSIRWDNLEYTEKAGPVAAVDVPTTTSVISPYASKKNWDKLDVEEDKSGDPLNNVFKDIFARGTDEQKKAMMKSFTESGGTVLSTNWDEVGQKKVEISPPKGMEAKKYEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKJ9</accession>
    <name>A0A8J4PKJ9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010119</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000932">
        <property type="protein sequence ID" value="KAF2068553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKJ9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="116" mass="13023" checksum="59083D825C641455" modified="2022-05-25" version="1">MERAQLWSDDGNFHILIDADNQTTLEKLNSSDSDILMDPPIPLCTTKRTTTTTTTSTTSTQTSTPTRKSTVTRTNYKGCNSEIIEWAKKTGLDYIFGGNLKKMEVCVEFYQTLTLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKK3</accession>
    <name>A0A8J4PKK3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000958">
        <property type="protein sequence ID" value="KAF2068495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKK3"/>
    <dbReference type="OrthoDB" id="14233at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1470">
        <property type="entry name" value="Peptidyl-tRNA hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001328">
        <property type="entry name" value="Pept_tRNA_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036416">
        <property type="entry name" value="Pept_tRNA_hydro_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01195">
        <property type="entry name" value="Pept_tRNA_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53178">
        <property type="entry name" value="Peptidyl-tRNA hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="242" mass="27344" checksum="A7FF8C8908EC9F0C" modified="2022-05-25" version="1">MNKAFSKTLTNVISAVRSKPDPIVMFGVGNFNSLSSRNSVAVRAINSLVNKLNMRWVDFPHIGAQLSYSPENHLLFVKSDTHLTKDNFEVLKRLIVLMPNIKTDTFCAIHYEHLYKLGMVESAYGGRTRHNDGLVGITSVFQTEKYHRIPIGIAHPIDDISFQTTPYSLGSAYEDSVTSFVVNRFPDVQMELVDKVVVPYAVNEIMSTIGKIKKDISQRENETDQQEKDLSAYKTSRVELKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKW4</accession>
    <name>A0A8J4PKW4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009973</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000869">
        <property type="protein sequence ID" value="KAF2068705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKW4"/>
    <dbReference type="OrthoDB" id="18713at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14560">
        <property type="entry name" value="Ubiquitin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="104" mass="11841" checksum="DAE483946F3F6BE4" modified="2022-05-25" version="1">MSMININVYCPSTKQTIKIENLTKDSSIYDLKRIIYDKTGTPPEHQIIQLNNKKIPNRYDSKKWEKLGINENTNIEMIYAMEGGCVGCRANLCCCGGSCRCQIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL96</accession>
    <name>A0A8J4PL96_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_008649</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000554">
        <property type="protein sequence ID" value="KAF2070030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL96"/>
    <dbReference type="OrthoDB" id="190105at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 7" id="KW-0853">WD repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="5"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="10">
        <location>
            <begin position="130"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="220"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="262"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="70"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="79"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2070030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="542" mass="61617" checksum="F648997697FE57DA" modified="2022-05-25" version="1">MDYNCWVCYDFFTEPITLHCEHSFCKKCIEKSSLIETLCPFCRAEYQLPLPPINKTLNKRMLKLKGFDVVDTDPEDNSNNNNNNNNNNNNNNNNNNNDQNNNDQNNNQNDIEIKPIPMDTSRDENIRYIGANILGMPREIYVLIFNNIPVKQLVEMQRISKAFQKIVNDNWVWKTKLHQYSQFSNPDKFNYDYRKCFIAYSKSENNTNKGAAGQFKMTAFRGHTQPITCFDHIGNIMTTGSIDNTIRIWDMNSKKGDSLLTLNGHSESITCLKQTQLNITSGSQDGNINVWDIESGEVVSNFTHPSGIHSIAYHPFYEGKMIGASPDLVNMWDVKSKIKVQSFTRTPGFVRKAQFDPSGKLMINTDQHFDVWDLRSPLHPLHRFNSASVYDVTPTHLYTWNTGILRKLNLVTGASELTTTILNVTNLKATSNDIAISTPQSVSILDAKSLTPKHSISNTNSMAINNLLIDDRKVITASQDNSIKVYDKNTGAKLYALLGGSNIVRSGEPTPLVQGCKEVRFDRTRVFGVFNHLIRIYNFRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLA5</accession>
    <name>A0A8J4PLA5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ABC3 transporter permease C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_008641</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000552">
        <property type="protein sequence ID" value="KAF2070045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLA5"/>
    <dbReference type="OrthoDB" id="2126250at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32522">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32522:SF3">
        <property type="entry name" value="ABC3 TRANSPORTER PERMEASE PROTEIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="424"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="481"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="524"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="577"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="611"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="673"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="970"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1014"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1061"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="domain" description="ABC3 transporter permease C-terminal" evidence="8">
        <location>
            <begin position="432"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="ABC3 transporter permease C-terminal" evidence="8">
        <location>
            <begin position="974"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2070045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1099" mass="125423" checksum="24BA4D9A74CFF680" modified="2022-05-25" version="1">MEQPNNSSRNNNNKNKKSSLNRSNNSIYYPFYEEFEVEDEEQQYIRQKQYFEKEKNENYYSFANQFHRKTKEIRSSLSRFGFFYLLTWKMVKKDLFSFFLGIFSCFFVVVVVAIGVTVISKIPIIYLNTAQNEVGEFDLVLRSGIQGDPSLVPPRLNFTLVQQLSEGLLEGYLAPRWESSSWLIKASGCMAGADPAFSDWKYFGLLVPGCEPGCMNYACSSAPQTYISLFAIDFEREADMQLGHGWPYPAPPKGTVYISQSLATALDLRVGDYVYMPFPASYYLREVWNATEEDKRISNSIPLTLKVSEIYSDSYGKFSEYYDDKSVALIDYKTFLPFLVEQASPGYFNTTIEELSKVNLYDLATTITYNFKNRIEFYMESDLDLLLRTIQTKTNKLLYRIGTRELNIELPVYQALIDSKNVSMFLSLVLNVLVLILVILSTFLIYSLLMVDVDSKTYETGVLRMIGSSKKNLFCLMLFKALNYCVFSILFGLLSAQLITYGVIDWFEKMTGQNIDSTLTGKAIWLSLSLGFIIPIISMIFPVRRALSNNLHDSLESNRSKVKSIIITIERASDSSFTINTIAIGAFLSVFGFGAYYIFPYSLVSGNLGFLLNSMFVLLIVLVLGLVLIALNFQQLLNRFIMLIFFFWENSAIKSIVLKNMIAHKLRNKKTSILYSVSLGLILLTQIIFIMQQNSTNFEKLSQYGSLIRIQNDVKFKQEDCAALEEYLDSLYPEVQDFSWFSRSQTALSKSGVPRTQIRNLGQVFMSHVELKSVSPNFFSNVQNHLVHLNKVVKDPDFHHRKPSDVSQQLYTTQSSNRIILGTTLETSVRANFDTPLLMVSSYVDIEANQNKSESDLIVSLNKDLVYPMAFMDFASPFRFSKFPTSDNQAALLSFPSYLAYTFGNVTSVRDIPFESILVRAANSDLESTTKLTSKIRNFINTKLSGYPYYVYDYYSENKGSRESVTMVNYFFAIITVLCMSISYFSLSSSMYININEQVKEIGVLRSMGISKFWIIRIYVIESIVLIFSSSIMGLVIGTFLGWAMIEQRKLITQLPLPLEFPWNLLLLVFALSIILGTISSFNPARRLLKNSIINNLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLI2</accession>
    <name>A0A8J4PLI2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">asparaginase</fullName>
            <ecNumber evidence="2">3.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_008562</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-asparagine + H2O = L-aspartate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21016"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="EC" id="3.5.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the asparaginase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.1" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000539">
        <property type="protein sequence ID" value="KAF2070122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLI2"/>
    <dbReference type="OrthoDB" id="427002at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009066">
        <property type="term" value="P:aspartate family amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08963">
        <property type="entry name" value="L-asparaginase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.40:FF:000001">
        <property type="entry name" value="L-asparaginase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1170:FF:000001">
        <property type="entry name" value="L-asparaginase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="entry name" value="L-asparaginase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006033">
        <property type="entry name" value="AsnA_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027475">
        <property type="entry name" value="Asparaginase/glutaminase_AS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041725">
        <property type="entry name" value="L-asparaginase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00519">
        <property type="entry name" value="asnASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006998">
        <property type="entry name" value="PRK09461.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707:SF28">
        <property type="entry name" value="60 KDA LYSOPHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500176">
        <property type="entry name" value="L_ASNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00139">
        <property type="entry name" value="ASNGLNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00057">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00917">
        <property type="entry name" value="ASN_GLN_ASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="L-asparaginase N-terminal" evidence="9">
        <location>
            <begin position="143"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase/glutaminase C-terminal" evidence="10">
        <location>
            <begin position="351"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="48"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="55"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="active site" description="O-isoaspartyl threonine intermediate" evidence="5">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="231"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="231"/>
            <end position="232"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001220-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001220-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2070122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="485" mass="53154" checksum="FC9A3F19242BA579" modified="2022-05-25" version="1">MSSPIINKSSSNSVNSTSKIHSHHELNHGYDMSTHQYLEVHGTSTHITDSPLNISIPNTTGSTPSTPSPTLECNQINNSSNSSVSTNSSNSNSSNNMHNSQHINISTNPGSGNIFEDIQINKLSVSGAKGPLHNSLIKMNRGNIFIIYTGGTLGMKRDPISGALRPEPHYLEEQLSALPEMKSLDMPVYTLTEFNPPIDSSDMEHEDWVKIAKLIEQNYYDYDGFVVLHGTDTMAYTASALSFMLENLGKPVVFTGSQIPFAELINDARRNLLTAIIFAGKVDLPEVCIFFNNQLLRGCRSRKVDSWSLDAFDSPNCPPLATLGMEINVNYDIVLNQPKGRFRVHEEINKNIAVVHLVPGFSDDTFENILSGKIEGLILQSYGSGNAPAKKSKFLQSIQKAVQRGVIVVVTSQCLRGSVNLKQYATGKSLLDAGAISGFDMTIETAATKLGWLLGRGLTKEQVKILMETDLRGELTKKVNKKLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLM1</accession>
    <name>A0A8J4PLM1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_010267</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001006">
        <property type="protein sequence ID" value="KAF2068406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLM1"/>
    <dbReference type="OrthoDB" id="69177at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF211">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="7">
        <location>
            <begin position="128"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="6">
        <location>
            <begin position="318"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2068406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="780" mass="89783" checksum="39EB3AECDDA6002C" modified="2022-05-25" version="1">MSVVSVPENRIITGSDFNSFETFQDQSFDHSLLKREDLGLSLSNGFTIDNFLTKDECLYYIKTSQDKGYVNIDKEFPKEYRNNQRYIGKNQDLSKFLWERLENILRVSDLEGIRPYGFDQKGIWIPVGLDDCFTFGKYLPGGRFKPHYDATFADNPDRRSIYTLQIYLNDDYQGGKTNFFLPENPLEIAKHQLEKSVVPKTGRALIFNHDTLHEGAEVISGEKYIMRVDMMFLRIDKIDQDSSEKEKNELAEARKLFFKADSFEKDDRDLESAISTYVKAQMLLIQYPSVDPVLLEFEKLKVSSTTTSSTKPAAPRVSLTLMLLPEVPLFEIVKQMPLETLLKLKSTSKYFNSTLRKNILWKSLYPRHFSNEAYTLENGIKTNENEIFTPPFKSGTSSVAPKSPSFFDYKLDYGVKLFGKGKKEEKEKPTTPSNEKCWYLVFKSTYLYRKQSMFTIIDFGSEQVKYCNMDSSFTIIPNKASSHMQYSPHYVMNTMDREYWNVGERASEFGAVRPVQQGMIDFDEGKAIYEIIKSCTNRLGITSPLYLIVAPTDMRNQDLIRKLPLRNTPLLIKDFGALVLKSHGLTNGVVLNCGFGSTWVAAYGDGVLKMIEELPFNGEDCNAYRDGLMGKEGSRQKDEFALLRKKKTAFLYQRVSQNYKQETQYDTKMLAFLSPEMYFNPAIAEFDCPNIVETCVSFIESAQKRFPDTDFTDLILTGGFSTFSGFEERFANELKAKNPNILPLFSSDRLFDALKGARIEHLLNRSQREQEQQKKQEKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLM8</accession>
    <name>A0A8J4PLM8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_009524</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the syntaxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000731">
        <property type="protein sequence ID" value="KAF2069155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLM8"/>
    <dbReference type="OrthoDB" id="19665at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006011">
        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF216">
        <property type="entry name" value="T-SNARE COILED-COIL HOMOLOGY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14523">
        <property type="entry name" value="Syntaxin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="4">
        <location>
            <begin position="164"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="105"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2069155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="261" mass="29157" checksum="CC5861F5A4FF24DE" modified="2022-05-25" version="1">MDFNKISQLLNEINDITQNIRKKKTVISTSSDSVDFRQGISKLIENGKSKINQGKSLFQQNYDRVDKPKVTKLTKQFNDLVKEFEVESTSIIDLQLKNAIGTGGRSGSLSINNRDDSSRSGSFAGRVQILSPPPQQQKQQYTTQSHIQLENLEITLSNAEPVEELIINEYNAEVKNLVTELTAIRDIAKDLEVITAEQREGLNAANTNIEVGKMDVEVGNQQLSQAYKYNTSRRKKIIILIILILIVIGLVVGLCVGLLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLU5</accession>
    <name>A0A8J4PLU5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008316</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000502">
        <property type="protein sequence ID" value="KAF2070360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLU5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="428" mass="47510" checksum="4E45CAFB7BF678AE" modified="2022-05-25" version="1">MTIRVNDPQDLTKINKNLRYVTQLSNSTSSQPIKSKASISVASQPRLYVPKDVRSAYLINNWVKLHILQEKASQDLAAVYDHITKHQTTAWITVEDMTTNSKFNMIWDSNGNYVWASCRGITYVTDPIDPQVHFVAPVTYGVFSKSTNICGVHAYSLSSATEITELVCAGIFSFFVGKLIAKGINFLADDIIAFLADSALELGFELVFSSVVAVSAVCCVAVFAIVFIGTVFLFNFLNKRFQISVSVYNWDTQVNWQVRNQSLTNAVNPGKPEKLDLNLDINKVSPAGSVVPIGNVNIPDAILEQVIRTCEDSFSYAFICYENVKTFAQGLSFSFQCTRNYDQKVGFTYAFECPWTASNAQYMEGTVQDPKAFLEKANKNWVKKTGPFTTSVSNDGSTIKAYFDMLQGGDQDGKDCYKVAVHINPPAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM11</accession>
    <name>A0A8J4PM11_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_009241</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000662">
        <property type="protein sequence ID" value="KAF2069438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM11"/>
    <dbReference type="OrthoDB" id="2162994at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="3">
        <location>
            <begin position="550"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="241"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="326"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="42"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="84"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="244"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="256"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="284"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="352"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="382"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="409"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2069438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1192" mass="134735" checksum="05DAD4342F14B706" modified="2022-05-25" version="1">MDPKRVYSIGGTPPLPFSAPSSSSNTSPVVISNSANSSVGNSPIITSTPTSPTIVSTTSTTISTNPTIISPTTSFKSSHSSPTLVSTNFENNLTGSTPGMISSGAGSTLPQILTPHKLPGNTATYQTNNGTIVYTVVSSQPSNTTQITQSSIPNGTITATPITTTTPATTQPQPLQVTSTSSSSYRPSYIAGNEAKISPKVKVEDLGPSFMYSQPIQSKPITTSFQNLEQQIDNMSNTSTVVMTPQQQQQQQHQPQPQPQTPTQPQPQQPVNRSTPTIIKQVPNENNNNNNNNCKNNLNNQQSPQYIVLPPDYSPKIKRRKSVENLENNNSNNNVNNNNSNNNNNQFYGYVSYTSSSSTNSKQNSPHSSPNTSPQYFEYCDSSPSSPDVNFKNITNEKHQPTQPMIIDTPSSSTTTTTTTSTSPTNATTSVGFTFPSNKNNATTTFGVMPNEQETWIEIRDLSSGISKMAKSSLTNGITEEMLTEIKEKGLVLMNMIETVTKKEKLERQLNDDDRGRIFPPLTRPRRFRKKKKMALEPANPNNLEKIKKKTDTLFCTSCGTTQTPEWRKGPAGGKSLCNACGLHYAKLMKKEGQLKVETTSSPPSTSMNVIMDQLFRFIFFKHVYLRRQIFDSVVNDVNCKTTNYKNCSNVNWMITNQYIDLLRCKLKYKEHLSMSLEKKFKDIATLKDIDIFTQVFQRFQEILHTDKDYIIGWCAERDNWKAVDFLFEQGYEYPDYLLLEYAYKTRNYKMTQRLFKKTEQVSKQTYIHAINSKDIKFVKLIFDHGRDSFGKESINFREEILWRSMEDGSLEIFKFVKKRFKETHSFVYLRKNFIYSKSLRSRLYLACVNSFETYIYTYNQYHEFFIKHQGSQSDSHYPPVVAARLGVRPVIEHMLENNLIPNHLVDQVIMSANGLELYTRLKNHFKLPLLPLNSICTLFQDLDETKNLVENVGAEITSKDLIETLPHSLEIFKYLFGKAGHALINQVQTLRYSLLLQATLLKDNDQVLHYLVVEQGIMVKPNDGILLWYDLVTSFPNNCFRKIQILLSRAPIFESELYMRVLECASEKCNNFKVFKLLLDTWLPLIQKKGKYLLPLFSNCLLAAVKNGRLATLKYLFQQGLFNASDFPLFLKAATLNGNLTMVKFLIENAPSSPLSIHHSVLDVAIDQNHHHLVKYLTPFFSLESSTSLQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMZ8</accession>
    <name>A0A8J4PMZ8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008431</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000519">
        <property type="protein sequence ID" value="KAF2070248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMZ8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="381" mass="43438" checksum="92E9CC7917F51D2D" modified="2022-05-25" version="1">MKGEENFESNSNDEEDSDDYNPIPEERELQVTCIECKLVSLALFELQEVSPVDHSTSNFLYFSSELCKKCNTPNNASYDRTYDVCKTDCSDQVSDDDSMPEENTHDFSSDTEDVENASFSSVNQGKGDCSILVVGQCLIIYDFGFKKEWPTLEKILQTLIPHIPQPHFAILILSHNDKDHKGNLENTIEFLNDQQFKVYLIARENENHMQVTNILSTDNDILSIHLSKNFYIKVHCPPNNGNENKKSLVAVIGNKDKIVVLTGDQYQDIIKKVLYMYKKKSIDLYQFPHHASLKQINDDSFSTSKYYLVSGTPLYDQNSNNNRGRNASCQIFCLKTIVKKKIQCTLILTQPRLSPEMTNYAAQNNIKILEVESCESFSLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN81</accession>
    <name>A0A8J4PN81_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_009179</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000650">
        <property type="protein sequence ID" value="KAF2069501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN81"/>
    <dbReference type="OrthoDB" id="411785at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000903">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051419">
        <property type="entry name" value="Lys/N-term_MeTrsfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12176:SF78">
        <property type="entry name" value="EEF1A LYSINE AND N-TERMINAL METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12176">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="5">
        <location>
            <begin position="53"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2069501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="222" mass="25448" checksum="01232FF594C531E6" modified="2022-05-25" version="1">MGKKEQIDRENISPSSHEFWDEFYETGEGKGDSYEWYVTFNQLRPHLVKLVKDKDKLLHIGCGNSLLAEDLIEETPTLSLEIISMDVCENAIARMNDRTAKLSNQRVKSSLQYIVGDATDTKYQDQEFDGIIDKGTLDACLSTLDFECGENEIVMKMVCEMHRVLKSKGFFIVLSRNTLLEQYFYISGDDLNWSLKTIELTTPSNKGKGITQVNYIYLLEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP74</accession>
    <name>A0A8J4PP74_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nuclear pore complex protein Nup85</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_007479</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Functions as a component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
            <location evidence="1 9">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the nucleoporin Nup85 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000402">
        <property type="protein sequence ID" value="KAF2071193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP74"/>
    <dbReference type="OrthoDB" id="17644at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011502">
        <property type="entry name" value="Nucleoporin_Nup85"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13373">
        <property type="entry name" value="FROUNT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13373:SF21">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07575">
        <property type="entry name" value="Nucleopor_Nup85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7 9" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2071193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="799" mass="93042" checksum="9F9320A3E2101E62" modified="2022-05-25" version="1">MFSTQSNSNNNKFVDFCWAPNGDMLLYDSIPETGKEKTSPFGSNNQKQQDDQVQYERKQILSETRKFYNEIFIQFKSMQIITSNNNNNIVPDERVREIIAMSKNYQSIMLATRDQMELSWNTGSFLDNEDCYINSNPFDKDSIEQQTERISKFMSENKDKDFIIFCGSIDSIYPYLCSKLSKETGMKCILIARESTPPSPKRNEVQNYIENNGNELVILSKQQYSDRYSFINQQYGDREGLSSFECDESIAKIQAEVDNFRVLSMVWKVAHLFYFNTSVMSPTHLLDCLQFERQELLGQMSNYIDVNSSSNSNHVNFDKSFYTYMANLLVNGCIEEVIHLLNILSRAPRHSSSIRTSTRRSPINIIIDLLQKIPLKKKTIYPNETLILWNKWHSEIQNVLSIYTQGTSSSSSSTSTLSGSIDDNLIEIIRILLGDQSTISKYCTSFLQLVVSNILYCEYTTSISQLRQLFIQCYNKYQSRSAIEKMLISFATKDLELTLKKALKHFPIWFVVHLSDLLYHHPYVMKKNYNEESQLSPIRQFLLSEYGQYLSSNGMIELGINYLENVKVGGKEMIDEIILRQPIHYEKDALRLISKASNQETKKSIYRMLSMNDFKRKRYASSLSWLMHSNDAPRVALLVNQLLEPEYLDSTLLQDLQSLMEKKESIPNFEISSELVFLVKYRELLNLWKDRNFKEYSVTLCNMFRDNLVPKRFWIRLMIDTIPLLESMKYCYFNYQDTTLLISCLEEIRLSHLFDNYSKGTSDQDIESLRLALSRNLSKSIMSTTLINNNNQITPIFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPE5</accession>
    <name>A0A8J4PPE5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007304</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000381">
        <property type="protein sequence ID" value="KAF2071388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPE5"/>
    <dbReference type="OrthoDB" id="10250105at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004077">
        <property type="term" value="F:biotin--[biotin carboxyl-carrier protein] ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835">
        <property type="entry name" value="BIOTIN PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835:SF5">
        <property type="entry name" value="BIOTIN--PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="284" mass="32245" checksum="0A86A70DE402B489" modified="2022-05-25" version="1">MINITTFPNSNTFWYYYSQNNNTNVDDFFISSKRPLLVKNLDEIKSRGFDNNSLPLVLGQNVPKDPTKFDFIIYFKILKTHLLGQNIIMNLELKNDVYLCLQLLSMTQQGLTILADQQTDINSGNYDTNMQRFVCPIGSLVFSFKLQRVDKNHSSLLEKLLGIAIVEALSTRSVVKQSKTEIKIQYIENSLTHPLYANNQKIGGILTQLSNNDDVFDVSLGAGINVHDSVFSLNNITGGSSTIILVSREEILATFFNNFESMYFTFINKGLEPFKDRLKLISKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPZ6</accession>
    <name>A0A8J4PPZ6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Right handed beta helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008714</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000565">
        <property type="protein sequence ID" value="KAF2069964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPZ6"/>
    <dbReference type="OrthoDB" id="77931at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31318">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31318:SF2">
        <property type="entry name" value="PECTIN LYASE-LIKE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035269944" description="Right handed beta helix domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="513"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="569" mass="63134" checksum="81A24ED3A688632E" modified="2022-05-25" version="1">MNFLNIILFIIVVVLSKRCDSTTVSTTTLYVDSNSEYLAPSCGFFLDKACKTIHDAVYSFGNTTDTNTLLDIKMLGGTYYDFQFDKNQYPMLDISNSNISISPLITDNNSLEVIISGQYFQTKFLNITSNNLQATGLEINNITFKQFSDSIVYFVPKYNNVVANSFIFNNIKVSNCKIVHPLFYFDKATYSYSYYSLYMYPKVVVSNSIFINNAIIPVSNPSYSYSTLSKTSIFSVWQTNVTMENNLFEKNAITSLYSLSCRLCLSNLTFDSNSYASSIYTSSSKSISIVNCTFTKNLVHSGHTDGAAYYSSFDSNVLIDNSTFNENYASYGGSIYLSQSQSVIKNSKFTSNIATLTGGAIYLYQGESAISNIDFENNVASAGMTIGVYGTSVSVTGSNSYFTLPYPTIDAASPNIISLTGGASFYAFNFSVADFPYSPANGGSHFNIIDCTRSTFSLYSSTNLTTYNQFDNNAICSGCYYHSDNSFKYNIDYCHTIDPQSPHPKRLSVHGKVGLAFLVISSILLLIGVSLIIVTLVRKDPYAEYNLLHNQFRFATLINDSDSDFDSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ20</accession>
    <name>A0A8J4PQ20_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Short-chain dehydrogenase/reductase SDR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007193</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000369">
        <property type="protein sequence ID" value="KAF2071498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ20"/>
    <dbReference type="OrthoDB" id="16849at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="56" mass="5975" checksum="AFFA4E4296904D28" modified="2022-05-25" version="1" fragment="single">MDPKQIIKPIALISGANKGIGLETAKSLVKKGYFVFIGARDTSLGEKAREEIGAQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ45</accession>
    <name>A0A8J4PQ45_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CUE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006723</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000322">
        <property type="protein sequence ID" value="KAF2071967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ45"/>
    <dbReference type="OrthoDB" id="20904at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20916">
        <property type="entry name" value="CYSTEINE AND GLYCINE-RICH PROTEIN 2 BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20916:SF18">
        <property type="entry name" value="IPT_TIG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="502"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="511"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="531"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="542" mass="60518" checksum="E386B06DC8185914" modified="2022-05-25" version="1">MNPSNNDSSSPLATTTTTTTTTSAPAAVNSDINKRNEKDDEMNNDDDSFDMDSNNNNNNNNNNNSSDKVTFSLPSIRPSKEEEEDVDKKDPANRYAPYKQKTNLSFEERLDMVELIINMFPLLEKDPDVYNILHKNGWDVEACLGQFQNRHEMAERKLEESKNIVDHSGGGGDGLSPIGKQRTTGGLSYMDKYPHPTSTREDYEKELEEMEEEESESEDERIMGSIGGVDPSTGEKSALDFSNFPNLRSDLQTLKDIFGTTYHEFELKSIYMVCGGNVEVVIDQLVNEQRLKDNKNNNNNNNNKNNNNNNNNNNSISSNSLGGNNYQPPPPTQKESISSITARLSEAEKRQLQLQLIEEQEKLEKNFSDKKQKEINAHPSKINFSQQEIVNELKDLFSKNVDDGVIEWVAYSCEYDLAKSVTQLVELKHNNLMKKGAIKNPFADPSVNMNIANNLDVLAKQMMSSMGASGSVNGASSTQTTPTNLVSQISFDSLVDEVQSKLSSADDTPINNKNNTTTTTTTTTKPFPFGSSMNQFKDNINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQB8</accession>
    <name>A0A8J4PQB8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_006632</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000315">
        <property type="protein sequence ID" value="KAF2072042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQB8"/>
    <dbReference type="OrthoDB" id="47374at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000006">
        <property type="entry name" value="Putative n-alpha-acetyltransferase 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051556">
        <property type="entry name" value="N-term/lysine_N-AcTrnsfr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42919">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42919:SF8">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="6"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2072042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="169" mass="19084" checksum="C6B78480C413399B" modified="2022-05-25" version="1">MAVKNVELGDLTNKNINQLKLLNSTILPISYDEKFYLNLLTNGFMTKLAFFNDVVVGAVSCRIDPPSDGCETSSLYIMTLGVLAPYRNLGIGKKLIEHLDTYCKSNKFSKIFLHVQVGSEAIEWYKKQNFQTESLITNYYRHIEPADCYSMSKTITPTTTTETNTTTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQE2</accession>
    <name>A0A8J4PQE2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006568</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000309">
        <property type="protein sequence ID" value="KAF2072123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQE2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035226317" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="156" mass="17870" checksum="72E54C8852932F3D" modified="2022-05-25" version="1">MGLLISIPILGVAAVASLQVNNEDRDRKEFFLLSQKIKTVELRIFYKRTNIFLPGSSLLLKLASGKFAHKSIIVTLVTGDMVIIDYELFELYDKNGRKNDFELDKSDLIGQCSPKKNLTLQDIKNKHVCYHLSRYSLLNDDSTDCATTLYNWLRDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQF8</accession>
    <name>A0A8J4PQF8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">pyrroline-5-carboxylate reductase</fullName>
            <ecNumber evidence="3">1.5.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_007749</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-proline + NAD(+) = (S)-1-pyrroline-5-carboxylate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14105"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-proline + NADP(+) = (S)-1-pyrroline-5-carboxylate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodecamer; composed of 5 homodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pyrroline-5-carboxylate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.2" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000431">
        <property type="protein sequence ID" value="KAF2070925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQF8"/>
    <dbReference type="OrthoDB" id="10263291at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004735">
        <property type="term" value="F:pyrroline-5-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3730.10:FF:000001">
        <property type="entry name" value="Pyrroline-5-carboxylate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000866">
        <property type="entry name" value="Pyrroline-5-carboxylate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.10">
        <property type="entry name" value="ProC C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01925">
        <property type="entry name" value="P5C_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029036">
        <property type="entry name" value="P5CR_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000304">
        <property type="entry name" value="Pyrroline-COOH_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645:SF0">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14748">
        <property type="entry name" value="P5CR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase catalytic N-terminal" evidence="12">
        <location>
            <begin position="5"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase dimerisation" evidence="13">
        <location>
            <begin position="168"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="274"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="484"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="581"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="952"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1015"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="296"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="308"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="382"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="415"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="431"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="441"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="484"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="499"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="582"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="609"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="644"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="660"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="686"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="711"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="730"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="751"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="771"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="954"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="965"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1015"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1045"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF14748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2070925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1075" mass="121385" checksum="050B092A5AD63FF6" modified="2022-05-25" version="1" fragment="single">MIDHIAILGCGNLGLSIAKGLIKSQKFKASNIYLTKRNIYTLDDMKKEGFNITADNVEAVQACKVIILCITSAQLEAVFDDINPHLTKDHIIISTLAGTTTEDIRSYVDTVEPVTIVRSMPNIAIQYCESMTCISIEASAKKDKSKDYALEVTLYIFNLLGTTLVLNEDLIVPATALCACGIAFFCRAIRAAAQGGCEIGFHSDQAIKLAAQTAKGAATLLLENGSHPESEIDKVTTPQGCTIAGLNKMEHQGFSSAMIMGIVTSAEKAATLFKNKRKQKQPKVESTNATTTTTTTPPPPPPPPASTPPSTNIDSTTNTTNNNNNNNNYNNTNTNNNNNNNNNDNNNNNNDNNNNNTNTNNNNNNSNNNYSTSSTSSTISTPPSISQPTTEPIIQEQISLPESSSYSIKKDQHSYNENSSSSSCNNDEYSPPSPPTPPSPSSSSSSSSSSSFGSTIAKSTNIQIQTQTPITILKSPILKEKKVEDEPVVEEKPAPSFFQPRSSLSSASLPFQPRSKALPITPPVTYDTYNVSTYSITSNSAVTSKPLLSSLSSLSSSQIASTPLVSKEALQDDLQDKFSKMLSPSSSINSNKQQPFLTKEDLRSPPQMNNSINNNNNNNSISKQTTDVSSDSSAFDSDPEFDEVSSVSSKSSLTSEQKKEKSRLKKEKKKLKKKLKKERKERKNAEAAALNNELYDMNHSASNNNNYKNSSYRDGHVDENSKNNSSLAWEEMKREAREARDAKDAIEKEKREKKKKEKEKKKKEKKKKERKEKERIKKERKEKEKKEKKEIEKERIEKTEIEEIEIVEIIEIEKETIETTEITIIETIEMEIETIETEPKEILEVVCQEIEIYQIETILETEIEKEIQEKTILEKETIETILEIEIEIEKEKEIGREGREEIQEKIEIQEEEIEIFQVMMNNQVIQCLIEIGIENLIAKEIEKLKEREKFDRKKKDKKRKRKTGKERQEKERQEKERQEMEERDRERDMQRDLRNSKEGKIVDKDTFYSSELRREELRREEQKRQLLKDREASSNRGNNFDDKNGFNNNRGHYDFNNNNNNNNNNNNNNNNNNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQJ7</accession>
    <name>A0A8J4PQJ7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007615</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000417">
        <property type="protein sequence ID" value="KAF2071061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQJ7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="294" mass="34753" checksum="BF421F3394175892" modified="2022-05-25" version="1">MTQPINYLLSNNLPHIVFKVIVDHCTGTDCMRLSVTCKGIRETITNIIQVEDSLIPFIDPNLDLLYAPINTLTRGCFWKNYYLCFPAIKFDTYYDDWFEVRTDKRCVLNDYQYLSTYVWEMEMGQVDIKNVPIGDYDVYWRIKVDKYSKALQFHCSANVDGQVYSFTMNTLDYKTIEKNTRQWCIYKTNKISMTELLSDDDDDDNSKRPTTMRVRCIVYNADEYRMSDLHIDCFHIVPKDRDLFRETLDHPSIIDIKQQTTYPLDSKNDFSHWVKQNDSFDFSDTTTSVPTKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRA3</accession>
    <name>A0A8J4PRA3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000360">
        <property type="protein sequence ID" value="KAF2071586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="24517at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140053">
        <property type="term" value="P:mitochondrial gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47938:SF35">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 4, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47938">
        <property type="entry name" value="RESPIRATORY COMPLEX I CHAPERONE (CIA84), PUTATIVE (AFU_ORTHOLOGUE AFUA_2G06020)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="164"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1316" mass="151799" checksum="CF9B11FA7E8A14EB" modified="2022-05-25" version="1">MYRIVGSFHLLNRLVKNGSNSGCGIYSTTSSSPSLSITTDRLYTTSSSSSDNSSSGTGNSNNINTRNINANNRASKSRIIDEDILTQLLSDYQGYFKENNLQAPTPTLPPRDTIDNSNNDDNKPLLDTDSYDINMYLNDKISEYYDKNIADNDIDQINFIRANRNNNNSKKSSTIGSPSSDTQDIKNKKVKPLKYKDNVNSSRETKSHKIESKILPRSQINVKPFIQNLDLPSKYPKMDKKSSLIDIFKSINHLDEYSKCCFFINNLNNREDTKPVLEELVLQDISYYPTLILYYALEEKFTSGSTIIESMTSSVINDTNNPFGICIMHLVHKMKIALIAGAIVTKNYTLVGEMMTKYQMHNLKESMFDIYQILHYGNHYLPLYVMYLHQQNIQIHQLLPLDMFLDSIYAIINLKPIAAIKWVDAMLLCGYDIDEEMYLDIHNILSSLEHNSIALSFRDNLSAKGYHPTETIITNSFLVLSLTTDVEELLSLYNNIEKYDVGQQVHDTMVATLLSHLTYSTNFHRLSKFLYKADSILSKHPSINIYHSLLTHYHSNQKMVKVLFEKLLKERSIEPDLSTIQILLDPLLKDNVRPKKALHTSLWLKSPIIKFTQADGLGMEVSHEDQDNNEQEETTTDPKLLQECMDLLHSIPIKDAKYYNFILLLSLKSVTPKSPLSILSEMLSKGFLPTSQVLTNLIKSLPSIDWIKICQQNNIPIDSTNFHIFLKHKSANFIESFYQDLKKSFPIMPETFQFLANTFLYLGKLDKVHRLIVSELGPLDDKEKANAITSLLINANNNIPLGEDDIKLLYQQIPDKAYHFAQCALNNKLYSIAWTIINSLPKHDEKTNEIVANYVYLYLLSNNTNIDVNDLLGKLSIAHLNNPMIRHSFMLAIHHNRSNYYNENGNFVPLPHSTFQLFLNHLHNLGIQLKTYELVLCGIIECVHSPRKKTKDFIMSSVANRIKGRTLDLLSTKLLVFNTAPLGIDHWIDNNLKYLSHDEVEKLMLKMLSNRKTLSHKTANQLLHRIVTSDPMYIMLFHYFNTQSISNAALCILDMHDPINQQHYLEIFINKHCKDQNYFTVYEHFEQSPNFDQKALEKFIELAAPLYPHFVLPLNEYLAFSTRYKYTISLTDSMATALAYYKRLSPDLYESIGLEPTSGFYVAMIKSLQQNRSLNYDNFIDIINQADERKLTFTSQDFFNIMMILSESPFTHNKKIFTFLLDFVGKEPYANEIFATLGKQLLTFIQKKVESKSLWTPLLFHEIINKYNDDKYSSSKYISLFIDWYPQYYNPQDFNIVTNEIKMKLRRHEFNQLGDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRE2</accession>
    <name>A0A8J4PRE2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Arf-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_006517</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000304">
        <property type="protein sequence ID" value="KAF2072174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRE2"/>
    <dbReference type="OrthoDB" id="10266696at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08204">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000009">
        <property type="entry name" value="stromal membrane-associated protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045258">
        <property type="entry name" value="ACAP1/2/3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23180:SF160">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE-ACTIVATING PROTEIN EFFECTOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23180">
        <property type="entry name" value="CENTAURIN/ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="7">
        <location>
            <begin position="12"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="123"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="544"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="143"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="196"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="302"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="544"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="570"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="596"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2072174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="610" mass="66450" checksum="299E1E863E687A57" modified="2022-05-25" version="1">MNKEEKISQACIAVLEDMLKLEENKYCADYPRWCSTNLGIFICMRCSGIHRSLGVHISKVRSVTLDKWNPDILEQMKNMGNQKANAIYEEHMPHNFRRPDQNSDAHTTEAFIRAKYERKEFMKRSGGDHGGRGGYHPNVYQPNHSNHSNHNNHNNHNNYNNNNNNNYNTHNESRSQRPTLSNGFSTGNTFMNTIANQNNNSNNNNNNYNNNNSYNNNNSNNNNNNNYNNNYNNNNNNNTNNNNFSNSKPTAPSASKSSISLLEDSSWTSPHKGSLIDDDFKSPQQPMKPTGSFDPFSSTSPNNNTNNNNFNNNNFNNNNNNFNHNNNNFSISQPPAPTNSFMNKPMNNNNNNNNNTGMGMNMGINMNSTTGSNSSSNSLLNSGLNMNMNAGGMGMNTGMNSNSNSMGMNSGINLMPLTNPNPMMMGGGAPMMGMNMNMNMNGMNNMNNMNNMNNMNNMNNMNGMNKPINMGVNMGMPGMNMGINMNGGPGHMGMGGMNNMNGMGMGMGMGMNGGGMGMGVNMNGGGMGMGMGMNGGGMGMNGGINMNASSPSPNPTTPSQFSSLDSLMFNSPSKPSTPSNNNSNNNGFPIASTGGNSNNNNNSNVNSLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS63</accession>
    <name>A0A8J4PS63_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005877</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000246">
        <property type="protein sequence ID" value="KAF2072803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS63"/>
    <dbReference type="OrthoDB" id="17470at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045068">
        <property type="entry name" value="BACURD1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING ADAPTER FOR CUL3-MEDIATED RHOA DEGRADATION PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145:SF8">
        <property type="entry name" value="RE57120P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="67"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="157"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="352" mass="40758" checksum="F9AD040D39AE2C76" modified="2022-05-25" version="1">MTSEATSFSSNKDDQNLFNQISSSIEGLNQIFKNEFQLKTERINALKERKKELLIVKKVMDESKIQETIVLNVGGMEFLTSKDILKKIPNSFFDYMLSGEIDIKPISNQPNCYFIDRDGSNFKSILNYLKNFKDNVTPVNLIDIKVREELVFYNMETFLTSKIITDRSFNIIKNWIGDLEFSLLYRASENRFEASSFNEKCNRKGATITLIEARCGDIFGVYNSASWGDFRIPEFDKTSFFFILSDSTSNKPTKYTYVNPFSENSQTQTEENPIESIVETKKSDSIFGRDIRNDFNFQEGPFIDTLGFFISDGCNNNFRSYRKGPSLFSGSLILKHNFIVKEYEVFKIFKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS96</accession>
    <name>A0A8J4PS96_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006415</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000295">
        <property type="protein sequence ID" value="KAF2072261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS96"/>
    <dbReference type="OrthoDB" id="423607at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639">
        <property type="entry name" value="F-BOX PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639:SF256">
        <property type="entry name" value="OS07G0242900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="212"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="62"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="126"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="161"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="737" mass="83667" checksum="6005382526527131" modified="2022-05-25" version="1">MDEENLTKLLQAFSSCNYIEFVKIFSMVHGFDATFEFLRNALTTIPIMAIKRKFFGGGNNDQQLQQQQQQLQQQPIVEEDRPTKRQAIRSTWEEEDNENNQFWTPKIFYDSDVDYHKNRLGHKNKSPNNNNNRISSSPSSSSPPSPSSPYSPPNQILNQRKNSLNSSNLKNNFVNSQKSKHIYNSNNKYYTPINNNSNSSKVYRKKIRSSRISNIEELPFEVIVYILSFLKYSDILIVSLVSKEWNRYSFVATTELTIENRKNLSVEIVNRKIYKHSNYLKKIRCCQLKYFNQQSISMITEHCRNLNDIQLYNCTLIGDAEIDILCKNLTKLTKIVLNSEIVSSKTLDSLCHSKLQEIELGGFSKVPYESFSKLGELSTLKSLDLTGIVNKSADHLCQQISNCKNLTSLRLREASEHAISLLPFLGNLVQLSIAEWLRPEIAPTTTTNQETMSVVTTVSSSVKANSTFQFLDKCKKIENLEISGHKDFILKDLNHVLSVANTLKVLDFDFSYCSDLDEKFYPMMSQLTRLERLFISKFNLSGDCWGAFSILPNLHTLSICCVKMNNHWINRGLEHLGACESLQKLSIINSTEHWITISEKSFSSLLKSSDNLFELRLGGSRGGGWILNEGVFMTIGKFKSLQLLDLSNSRGITESCFVYLSECLNLESLEGAIKVDENTLRSTIAEWCGTGTLVNCPSLRKVSIDLQLPSFINSEIAPENGGGTFNLMAKISKHSPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSI4</accession>
    <name>A0A8J4PSI4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006531</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000306">
        <property type="protein sequence ID" value="KAF2072155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSI4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="129" mass="14920" checksum="5C9E477DF9B445BD" modified="2022-05-25" version="1">MPRTKTRVRDLEKEKKTYEKTIHDQKDIIANQEKLIQELEKKSDEKVPKLKNGIIDPVSPPSGHQGNPPENMDHGVLSPTKNLEKSPYVLEIKLKIPQDGTYSWTSWVSWAQEIFSIIKQIVQKLLKSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSR2</accession>
    <name>A0A8J4PSR2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009894</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000845">
        <property type="protein sequence ID" value="KAF2068786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSR2"/>
    <dbReference type="OrthoDB" id="22524at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF178">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="891"/>
            <end position="933"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1121" mass="128770" checksum="00026FD4847DDE82" modified="2022-05-25" version="1">MSDQEILKSIPKTVKELVLYPYSNMLYFDSKYIPATITKLRVFHQFHINLLNIDKLPPSVKSLSCTDSKLSVCKIPSTVEHIELTNYLVRKNYDDLFQRYGDLDNYSNYLACVEQSNSFKAFTKITKRREFPDNNDNDTHIKSRISQWSMEDEFRKDIKNVTLVDIAAKSLLLLLKTATATFNQIHLVVLCFNDTKFLKDLTQIVDLLKDVVVSIKVIRNQKAEKRASNELVKRFKQHIKESTLVYQSDYLISFDQFKEFLVMEDTDTPLDEFFNYSFGGPGDDDILPNTFDLIWTSVVSPKEYEIPNGVKKLTFTNIKSIRGSKKVKSFCLWPFGYTIERKSFRINIGSKYCTPLVDIQRNAVKRHHHLKLNYPYGKMEQLLDPFLIPTSVHCLDIGADNLINFQMVDQKEYYRDPLSRDSFKSIANLNSIELAKQWDLPITINTFPIGLQSLSFLDVYRCSSIEQIPDHIKLNYLEYPIGHTKLIFKSNDRMITKEKIPIEFDESTGVFTPQPAFPIDELFLQLFNGKIIIPMPNADIRKVKCNQRMRLPDSVQVYVSNYFLPVNYPPLLEQLYLEFDEPGYKKIELLHSIPPTVKYTSFRFYSTSNNNIFKAIEKYKLSSLDELMKYNSVVINKDSFFQIWNVFLQKKINKMAQPKLIYTQKTIANGISVYRLQDPKSSSHFKFKTDYISDIPFVLESLPFGNIEINSLLIPTTVETLEIKACIGPPFTNVQLLPTTLKQVIINTKTLQEQDHVYQIYDQSSTGIGPLTTTLVWQANELIPVGFIPNGIKRIVFGNNFNTTILPNTIPNSVTEISFGNGFNQCLSLLYLPVSLKYLSLNGYKHPIVQNSLPLNINQLCLSDYDSRLISSCFFPKSIKYIKINNKRYRQNNLQQIKSNYENENRELNIHIHDHLNNNNNNYNSNIKSTKTKIYIDNHPIVPGSLDHINIASISFGSLYNQILLKGAIPDTVTELDFGQSIYNQPILSIPTSVQILYLGSITTDIESIVLPSSLTVLYLPNTFDSPIPTGFLPHGLKVLHINKCFKQIVPAGFIPNTISLFPHQDMTSLDFIPRSVGKLQLNGFKSHHSNPLICTIPQNVTELIINTLVSFNSYPHQLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT06</accession>
    <name>A0A8J4PT06_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Actin binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_005397</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the alpha-actinin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000214">
        <property type="protein sequence ID" value="KAF2073298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT06"/>
    <dbReference type="OrthoDB" id="10017054at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043226">
        <property type="term" value="C:organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21214">
        <property type="entry name" value="CH_ACTN_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21216">
        <property type="entry name" value="CH_ACTN_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000001">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000036">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000178">
        <property type="entry name" value="Calmodulin-2 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014837">
        <property type="entry name" value="EF-hand_Ca_insen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08726">
        <property type="entry name" value="EFhand_Ca_insen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01184">
        <property type="entry name" value="efhand_Ca_insen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="14"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="128"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="721"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="757"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="315"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2073298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="853" mass="96805" checksum="2158633634B5CD75" modified="2022-05-25" version="1">MSSDKQLLDKAWERTQKKTFTAWCNSHLRKVGSSIETIETDFADGIKLSQLLEVISNDPVHKVNKAPKLRIHNIQNVNLCLKHIEDHQVKLIGIGAEELVDQNLKMTLGMIWTIILRFAIQDISLEELSAKEALLLWCQRKTEGYEKVKVSNFHTSFQDGLAFCALINKHRPDLIDYNSLNKDDKAGNLQLAFDIAEKELDIPKMLDVSDMLDVPKPDERSVMTYVAQYYHHFSASRKAENAGKQVSKVLDSYMTLEQTKFDYIKRATDLVEWINAKTQQLEGRDFGESIESVQNLMTAHKDYKNSEKPPKGQEVLELEAIYNSLQTKLRLMKREPFVAPQGLSPTEIDAVWSNLEKAEQEHAEALRNELKRQKKIAYLLQKYNRILKKLEGWVASKSNYLSSSDFGDSITAVQAKLKNLEAFEGEYSSLEGQSNTDLVSILDQLTELAYNGVAQLTERKDTFFNGQWSQVKQSADQYKASLLEELAKLQKIEDMLVDFAKRAAQLNIWIESADDQVNDPINADSVEVVNEIQAKFDAFVQEQAGQFAELESLAQLTQELRNLGRSENDYSVISYDDLSKKWNDLLAAIEERKASLCTELDTQTNNDALCQSFAEKANKVNEYTTTQNAVLSENNSSDPQEQLKNIQNVITSQQENKPILDELVSVQAQLDDAQVTDNRHTQLTLESIKMKWEKLATLSRKAEQVVQGEILAKQHTGVTAEELAEFKACYSHFDKDSDNKLNRLELSSCLKSLGEDLTDDQLSAVLAKIDKDGNGFVSFEEFVDYMISSRKGADNAESTKEAFKIMAEDKPFITEAQIRQSITDAKQVEYLLANMPKTDNGYDYNAFADTLYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTA1</accession>
    <name>A0A8J4PTA1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005596</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000227">
        <property type="protein sequence ID" value="KAF2073102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="21565at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="722"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="578"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="660"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="797"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="927"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1023"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1094"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1349"/>
            <end position="1445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1529"/>
            <end position="1548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1585"/>
            <end position="1609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1833"/>
            <end position="2042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="114"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="367"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="454"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="667"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="801"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1023"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1095"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1113"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1349"/>
            <end position="1373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1398"/>
            <end position="1445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1530"/>
            <end position="1548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1585"/>
            <end position="1597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1844"/>
            <end position="1854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1862"/>
            <end position="1875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1882"/>
            <end position="1924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1936"/>
            <end position="1964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1974"/>
            <end position="2038"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="2142" mass="226776" checksum="4CC2BB385F391C9B" modified="2022-05-25" version="1">MVSVDTYSLLVNNNNSNISSNNSSSNNRIGSNKNYINIDIMKVNGRKNRNKQQDNEDEEIDIMNDSDDDLDVTGDADENRVIPANPTTTTTSTSSSSSTAASASSSSSKKKKSTTTTTTTTSTSTKKNNRLSASSSSISTTKKSSSSSNNNSSSKKSSSLSKSVDHTNTISDSSDIETEYIHTKRRISSQDSIPLSASLQPLQQDHMFLPPSVSMSPMIDVISSQDVMNPNFGLNSSYSMMDIYTLPTPTLGGLPSDFGTPLDEKINHSFIENLNTLNNLNNSLGASSMTTTNNNNNNNSTSSSSSKNKKRKSKLDEISTNDVEKDNHVNSNDNDDSHNSKSSSKKIKVSRSGGSADKKEEEKKSSTSKNKLSSSSSSPSISSKKSSSTSSSSSSSSKSKSQSKKLSKSKSKSKSSSSSLHDDEHLSDNSSDMDVNISDYDSSGDEGPPMPMYSTPKSSSTIKSNNNSNGKSKFYESDTEILHLPSSTAVTLVNNKNTQHYYGPLSSISDPHGFQSLSFLAPFSPVGFPIASPMSPSVMDFAPLPSACPLSPLSPLSPIMSPVSPSYINSGMSVGMGNGAGAQSHAGEHISSPSDSPPPTPSIFSNCSVFSNILATSNNGIGASATDIFNSPLSPNSLIKSINAAEDAKSNSLVPSVSATSIPINGSPNSTTVPSTSASTSSPTSTPPTTTTTTSASNSIVPATSKRTKRFTMDPHVQKHFQPTVWYEEEKLFFKELFCAYGRDWQIISTLMCGTKSPTQIKNFYYDVKLTLLGPLFGHSDSDTKARITLPKNESTMIVKSDSSTSIMPPPSSSLTKSSSSISTTTSPPLNINTTSKRLTAASDDDSRVKKKSRTTKRIFRFSRITTPVTRTPKNVPKPSKLKMNETPSFVKYTVGSALFYFYENPETLPVGKWFEVKILEWENINNSNGSSGNGKNKNSSTTPPVTTTTTTTTTTTTTTTTTTSAMDISPLAVGSSSILDGLDININFSDLNPLAVDSAIDSYTNSTIDTLLQPLTPLSASPSPTTTLTVTTTQVTPPPSPTLPPPIIRYKVVMFNMNKTKGFWVKEEELKLELDIGQEIWEFSADPLADEENNNLKEKESKEVVKETQQPNTTKNNNNNVVENETKSLTTISNSSKSIISNSLTTTTTTNKNQKLGQLYYSQIASLMPAAPPTTQTSQELVSRLVQSLADSMDRYKLQQLLEAKLETVTPSNNIKYPTLEYDPKLISKELLDIPAFSLEYLNNSSNSIVSKQRGFFESISNSFEEKEFVSINFNSIFSTWQYIEDINDRSIIIQMFDNVLKASDILVTNIIVKPPVNNINNVNSLSPTDPVIKKEVSVDSVTTTTPITNTTTIPTTTPSTTSTPTTNTTTPVVVKNEDLAKPSTSPTVIPVSIKPSTNNTTTTQPSSTSTTPVTVAPQQTVSKIPPKPITTTTTTTPTPATNVTTTNSVVKTTLTQAPIPTTTAPITPNTTTSTVKPIPLPMKPVVKQSPQLIINKPAAGVPNLAPSTITAKPPAGSTTTIVRPVAGPAPQQVTTPTTASPTSTTTIVNGQTTTVKHVYVKPPAYSKPPAGTTQTVVATTTSPTATTTGSTLVAPSPAPSPVPAPVSTTVAKAGAQPQPQPQTIVTRSTTGSLNNPLVVAKPPAATTTTAVVNGKVVTVSSAPVVMTSTAKVAPSSNVVTTTSTGIPSSIPKGPLVGTTIARSPTAGIPNTPSNIAGVISKPPPTTTTAANGVVTTTTLSNGQIPPKPTSALAVPPKPVTVNNKSLPKTIITSTGQKIYTCTCCLSKNPPGVIVSPQYKISAKGTPTTTTIITTTTAAGKPIAMTSKPPTSVIVANHPAGAGTTTVASSSPALQPKAPMPTSQTLVTSQTITSAKPIAQPQPQQQQQQQQQTHQTPQLKSSTPQPTTTTTSYPSSTVSTTSSVATNNRISNVKPLPTTTTPALSTTATTTATTSASASIATPSPTPTPTPTSPTTSTSSNLPNTSSTTTSPSNPSTNASATSSVGNVSSSTPSTTTPSTPSTTTIASPPSSTNTSSKLTYSSGSTNTNFNILFNPDKSPIPYRLFIGKRELWLECRRLLRIQCSRISDLNKQNIESGKDTNEVICRMKELTSELSAFKYLLLELAAERYEVYKKSVGVYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTT1</accession>
    <name>A0A8J4PTT1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosome biogenesis protein SLX9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_009354</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLX9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000687">
        <property type="protein sequence ID" value="KAF2069326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTT1"/>
    <dbReference type="OrthoDB" id="18703at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028160">
        <property type="entry name" value="Slx9-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31109">
        <property type="entry name" value="PROTEIN FAM207A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31109:SF2">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN SLX9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15341">
        <property type="entry name" value="SLX9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="184"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2069326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="210" mass="24228" checksum="996A891D072D4294" modified="2022-05-25" version="1">MPKESKQNGKKQQQDDFAMAEKTSFVTVPKLDSSKLSMGFQLDKTIIPSLSTKIDYNQNDNLIKENTDSRPQPISKKEKRKIKKDQFLQKFNPTLNLNINKKQMEQDRLDLSKSGDFVSSIDLLKESISIPSKKKAPKMTNEKKKKISIKESNQFKTVLAHPVYQSNPFTTLQEHLTNSIALQNQKIKDQQDAHKALNRKRKTNNNKMDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTV6</accession>
    <name>A0A8J4PTV6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_009315</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CCC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000677">
        <property type="protein sequence ID" value="KAF2069371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTV6"/>
    <dbReference type="OrthoDB" id="73465at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008217">
        <property type="entry name" value="Ccc1_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01988">
        <property type="entry name" value="VIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="248"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="279"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="309"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2069371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="38078" checksum="64CDD93C74951551" modified="2022-05-25" version="1">MISKNSDLSPSPTSSSSSSSSYFFENININNNSNTSSEGSRLTLRRNLDVATEAYKTNDVRKSKEAHEIRKIEDEPHKSNSFKKGSTKYIKTATTFGSYSILLCISIVMGLHTYLNEKNNVNSSNSNNSNNSNIELKVYNHMILFIVTASLFICGIGLTLIESTNRKIEHSFYHSEKRREEWEYDNYIEGEQSEMVELYCIKGMTEYDAQKVVKKLSKYKSLFIDIMMLEELNLMPVELLLKPMQTAFATFLSFVVVGLFSLTPFFVSCFITFIPIQLIYSSFLILSLMVLFIFGSIKSKFYTGIWWKEGFYSSMAGIVSILMGIGFGTLSTTFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVD7</accession>
    <name>A0A8J4PVD7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Rab GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_004779</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Lipid-anchor</topology>
            <orientation evidence="7">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000175">
        <property type="protein sequence ID" value="KAF2073925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVD7"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01869">
        <property type="entry name" value="Rab1_Ypt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000359">
        <property type="entry name" value="Small GTP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057289">
        <property type="entry name" value="Rab1/Ypt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="174"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="193"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2073925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="22555" checksum="C33E76939DB46C86" modified="2022-05-25" version="1">MNPDYHYLFKLLLIGDSGVGKSCLLLRFADDTYSESFISTIGVDFKIRTIDLNGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDKLTFENVRQWLQEIDRFASEHVNKLLVGNKSDLVAKKVVDYNTAKAFADSLQIPFLETSAKQSTNVEQAFMTMATEIKNRLTASQPSAVPDRNKVVPGSSAPISPKSSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVP3</accession>
    <name>A0A8J4PVP3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_003807</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000124">
        <property type="protein sequence ID" value="KAF2074888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVP3"/>
    <dbReference type="OrthoDB" id="5511210at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="5">
        <location>
            <begin position="59"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="22"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2074888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="178" mass="20300" checksum="C4E345CE0A0F5AC2" modified="2022-05-25" version="1">MTTLYNLYDFINYIERSKENEKVASKKQKIDHNNKSSPNSNTPTTSSTTTATPKYKYQQPIPSYDPNVDIYETKDTVYIELEAPGIEKSDFNIDWNSNTSKLIISGVRKNKISQLNNDNNNSKLLVNELEHGSFKREIKLNSNLIDLTTINANLVDGVLFIKISKKPQNTLNIKVNIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW22</accession>
    <name>A0A8J4PW22_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_003913</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000130">
        <property type="protein sequence ID" value="KAF2074767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW22"/>
    <dbReference type="OrthoDB" id="5511210at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="5">
        <location>
            <begin position="147"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="13"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="43"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2074767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="263" mass="29385" checksum="866B4CA623A2FC6C" modified="2022-05-25" version="1">MANLVFTPFSFLNSQDQAPHNFGPHQGDRFHHERSDSQGPHSHGPHHRGPHHRGPHSHGPHSHGPHSHGPHHRGPHHGDRHERPSFGPIDFLFGGHPFSHPSQTSPSPSPTASPTVSPTASPTPSQCQREKSNVLFGCPRSQPQTDKKEQTFHPDFDVYEDNEFVFIELEVAGLAKEDINIDWNTADSKLVISGEKAKKELDPSQVVKRITQERKFGSFNREVVLNANYIDLNSIEATFKDGLLLIKIAKKDESSLKVKINIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWS7</accession>
    <name>A0A8J4PWS7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">40S ribosomal protein S17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003409</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000106">
        <property type="protein sequence ID" value="KAF2075280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWS7"/>
    <dbReference type="OrthoDB" id="1727351at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.60.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.20">
        <property type="entry name" value="Ribosomal protein S17e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00511">
        <property type="entry name" value="Ribosomal_eS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001210">
        <property type="entry name" value="Ribosomal_eS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018273">
        <property type="entry name" value="Ribosomal_eS17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036401">
        <property type="entry name" value="Ribosomal_eS17_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002242">
        <property type="entry name" value="PRK01151.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00833">
        <property type="entry name" value="Ribosomal_S17e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116820">
        <property type="entry name" value="Rps17e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00712">
        <property type="entry name" value="RIBOSOMAL_S17E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="15448" checksum="0D3C7A5B53425FCC" modified="2022-05-25" version="1">MGRVRTKTIKRASKLLIEKHYPRLTNDFDTNKRTCDRVAKIPSKRLRNKIAGFVTHLMRRIEKGPVRGISYKLQEEEREKRDNYVPVNSAIKTDEIRIDDDVDQMLKSTLDFPACPQIKVDKKNKRRPQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX91</accession>
    <name>A0A8J4PX91_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002162</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000057">
        <property type="protein sequence ID" value="KAF2076548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX91"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="420" mass="49466" checksum="A422A0FCAB38AF13" modified="2022-05-25" version="1">MSFYKPSHQDLKYYIEKRESEIYVRYGLRKDDLDNITKAFMSFEDFSKFIVTYYEFTKTEEAKKKGSSFFRQESAQKLFNKINGITTINQCWNFQKLSEADNNKKGQLEKKIQDAIKKKTLLELSDKNPEVKKYFEEVRKTLSNAIEKGKVFFAQANQVREKFNEIFTLLKIEKVEWESVSGRVSCITQKSECDVCSKGHRSSYKWRCRYFCLYQKPKEDIGKQLGLFSMLTILGLIYQNKSQDDLDVMSKKFKAESPQELFLEVQCKIGMYPEIYYFLFNLAKNYCLSKAKHKTLKNENLVEKLSKGKEYTPYSIRALIDSYLDEPNEANRKIFLDKLHLLNSPQLKNEVFKKDGAECYKMTSFSDGVYLTTTCIVKGTQIAHILVYKEDFINGNKQINKEEKTLSKEEIEKEFIDEKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXC8</accession>
    <name>A0A8J4PXC8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Leucine-rich repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_003672</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000118">
        <property type="protein sequence ID" value="KAF2075020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXC8"/>
    <dbReference type="OrthoDB" id="1687175at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22710">
        <property type="entry name" value="X-RAY RADIATION RESISTANCE ASSOCIATED PROTEIN 1 XRRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22710:SF2">
        <property type="entry name" value="X-RAY RADIATION RESISTANCE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="197"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="515"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="515"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="544"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2075020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="649" mass="74484" checksum="4D8CA6ADBE9F67FC" modified="2022-05-25" version="1">MIDSNNLIKDIYYKQQQQNHHHHQQHHHNQQQEQDSDDLVYYKSANMMTIDESLDADTESEYINKELEIQKYIENKKIFKKRYSSSTSSPVSITYLQTIDSNTIDSMDNYNQYHSYQQQQQQQHHHSIQIDQYQYLQQEQYLTNNIYPSSSYESSNDIDFENQTPYKLSSLDSSINTPIPLSSSLSSSKPSLQYYNSSTSTPLSSISTPSPSSKRSSLSKSSTILYSKSPQTKMPFLSHSKSISSIQTIPIPSQIIATPRMKIKKAPSFSGFPQQDEMILLDYYFIMTYTKAKDNHNVVALELPNLHIKSVNNENFDLFDKLSYLDLNNNHVKFNTLSNFPNLCELYLSNNLIKNLEFKDGFNHLKKLDLSNNSIESNHIYQLMKIKSLVCLNLSFNQIKILPQDMSSFTNLETLYLENNFLTIKTTMDSLSTIPNLRLLNLNGNQWDRILPISGCFQSLCELSLCNNFISNLISIGNLYHTSYPLLTRVQLSGNPLKSTNFNFQVTKTIQLITDPPVSSKSSPVRNKTPSSFPKENRKKLTVKSPSQPSMTPQANNSINYNYFNNNNSGCRSVLQSPVTFMENDHFQSTHPLSKSTPPTINNTINSFQDLNLTDSNFTPYKPPMNFKTAQRFNHNIGSSEDYMDYSTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXR8</accession>
    <name>A0A8J4PXR8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_004663</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000168">
        <property type="protein sequence ID" value="KAF2074049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXR8"/>
    <dbReference type="OrthoDB" id="10253869at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050218">
        <property type="term" value="F:propionate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43347">
        <property type="entry name" value="ACYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43347:SF3">
        <property type="entry name" value="ACYL-COA SYNTHETASE SHORT-CHAIN FAMILY MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="4">
        <location>
            <begin position="14"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="2">
        <location>
            <begin position="78"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="3">
        <location>
            <begin position="530"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2074049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="653" mass="73484" checksum="5FDC6B369884AC4C" modified="2022-05-25" version="1">MQQVDSSENNLSLYEKEKKLFLENPLKFWEQISTNIFWDKTYDRVLGGTDESPLWFENGKLNICYNAVDRYALDPLTKDKIAFYHETASMEKTNSITYLELYERVCEFSTALKSIGIEKGDIVLIYMPVTLEQIIAMLACVRIGAIHNVVFYGIPAHQLCKKIDLFKPKLLISSNIGLFGEKILLSIDILNDTLKLSCHKFHVVVYQRKDIVSKYSEKVQLQFSSKFLDWSDLVKKATENQLINREYTLVDSNDPFSVIFSTGTSSGDPKPLLRSTAGYVVALSYHLRKTYNLTTKNDVIFSKSNSSWVSGQSIGVYGPLLCGATSLLHEGDLKVPNIGSTWKLVSKFQVNTLHIPPNLIRAMNLLDPKAQYSKQYDISCLKCIVSGSEKIYGSTISYMKQICNAQVYQDYWSTEAGCPMISNPGGIFNIPIDMTGVIVPGNNLLIVNQNGQTVKREEVGELVVKLPIPPNFAFSIYNDHSRYIKSYLSHHPGYFSTGDLAMETKDGFIDVISRAEDCQTINGVSLSITKIESLLYSHPAISECLVIVIKGVEKRDITLGFTTLGSNLSKNDVEKIQKDISSLVEGTFGPSICFKGLLFIDRLPKMQSGKIYRKILHDVFNDISFEIPDSVLENVNVVDELKQVYKVFKANNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PY92</accession>
    <name>A0A8J4PY92_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_003574</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDC6/cdc18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000113">
        <property type="protein sequence ID" value="KAF2075139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PY92"/>
    <dbReference type="OrthoDB" id="1926878at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049945">
        <property type="entry name" value="AAA_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015163">
        <property type="entry name" value="Cdc6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF26">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09079">
        <property type="entry name" value="WHD_Cdc6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="56"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="13"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2075139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="409" mass="46106" checksum="96017C489497054E" modified="2022-05-25" version="1">MQTLLESLNEKFSQTEDVIGRGDDGSGGDSSDSIPSRSKELKKLRSLLKSKGRQLTGDSLYIAGTPGTGKSLTVKTLLRNISKNKDWMTVYINCMDLKDPSNIYIEIYKKLKKTKSTTKSKDKCFEGIKEIVTKDKMIFLVLDEVDFLISKYSSILYDLYECPYIKDSKLFLIGIANTLDLVYKCLPTLAKKGIEPTVIEFKAYTSEQICEIIKYRIEQTISDLGIEDEGFSDKIIEPDTLNFLCAKVAANGDIRKALQIVRRMITEKFESLGLDQDNNDDECTISFEGEHLFTLDDLSQIISDTFDNQTVQNIKTLPVQAKVILLSVFRTKQNLKISTLYKAYISVCKSVYLSVLSLTEFGDVLNSITPTGLMDIIGNDEQIVIHFSTEDIKEGLSNQHIFQNLLDTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYG3</accession>
    <name>A0A8J4PYG3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002644</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000075">
        <property type="protein sequence ID" value="KAF2076030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYG3"/>
    <dbReference type="OrthoDB" id="16772at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000323">
        <property type="term" value="C:lytic vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035493">
        <property type="term" value="P:SNARE complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018791">
        <property type="entry name" value="UV_resistance/autophagy_Atg14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15157:SF12">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15157">
        <property type="entry name" value="UV RADIATION RESISTANCE-ASSOCIATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10186">
        <property type="entry name" value="ATG14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="320" mass="37308" checksum="FCC80E7C152B9E72" modified="2022-05-25" version="1">MHSDQSRVNELLQEIFEKRKLNVVAKKQIQEIIPTLEKQNQIEGKITYLKSKRLQLEKLREKINSLKGDIDKDNKFIDAKKKYLSSRRSNLKKSKVSFENVNSNTDFLSIESDIQSKKQLVNEKNMTLESLKKQRIEKIVNNTMKIQISQSNPDHFIIVNIVINDKTLLKFPKDIVFTSLGYLVKILNLVTGVLGITLPYQMIYKGSKSLIQNDEKIKEYPLFYQHKTKSRDFQKALFLLGENIIFLRLYHGLSTPKEMSTYFIKNLYELFKSPNLGKVQKISEISSSMSTDIYDDTKEDEWEVVASIEDSDEAYENFVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYK5</accession>
    <name>A0A8J4PYK5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000027">
        <property type="protein sequence ID" value="KAF2077508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYK5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="282"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="377" mass="42921" checksum="782F721D42B7A9EA" modified="2022-05-25" version="1">MTHNQKDDIVLCRNDPSTRSLALGELFKDVSSSLNNSNNSNSNSNLPSILLKMIQGQLDDIVNKPNNENLLLEIDWFRDILLKSFTYFTTDTKDKKSKKLFLSSLVKSTFKSIDDIRLKYKSNSIINSNNNITPDNSSNNNNNNEKNNSQGIMNNSQNKKLIDYELQVILRLEYMIYLFQSSIAFKKSNNSNSNSNNNSNNIYDKEYCNSLLSTPLINAIVSLLQDISFILDSRNSGNFVDFLDTVLVYKYLHSLPYTLSKIYKLLEISKPHSLKQYLKQINKQPTPTKLNNNNNVNEKKKSIPMTPIENGDENDNTSKKQKLDSKSTTTTSTPTPTPEQEAQSLLGIKRVNARRLGHFNMNLSDPKAFKTFKTTTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYM2</accession>
    <name>A0A8J4PYM2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003340</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000103">
        <property type="protein sequence ID" value="KAF2075364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYM2"/>
    <dbReference type="OrthoDB" id="4405280at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052326">
        <property type="entry name" value="Diff-Dev_Assoc_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33459">
        <property type="entry name" value="DD-GDCA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33459:SF7">
        <property type="entry name" value="DD-GDCA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035284504" evidence="2">
        <location>
            <begin position="22"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="405"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="440" mass="47892" checksum="BEBFD61C10062E50" modified="2022-05-25" version="1">MYKYITFILLSLLLFITIVNSTSFTCSESRFPPFVARNETCASKGPFCKPYLQCNLQSTKCERIYSNETCVNNACGDASLTCIDGFCRPKGFLTFGSACNETYQCMSLNCANGICQNSPSSASNTQCSTNSQCNTGICVNSTCNVYSCSGDEQCPNNSFCFESKCKAKVEKGGVCGSSVCQMPYSCSYQNSTSTVKTCIEPLSLQQGDRCDPITFLSHFDILTYPCDISKGLYCSIQGICTTYVQTNDTCPYDRECTYTEICDCNSYTSPTDKVCKPVVNFNANCSKEVAIVNECIANNTIIMTDLVTQVAFSPLYDKCGTKLCELNTQCFIAPSSAIMYYCDNYYPHPPRNPAAVHPTAPKAATVPAAAAHPMTAAHNHPAMLPTHLHTTRPSPPTRPPTRHPAQPPPAPQPPLAPQLAKPTSMTPTPLVHYPSHSHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYN9</accession>
    <name>A0A8J4PYN9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carbohydrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002099</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000055">
        <property type="protein sequence ID" value="KAF2076613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYN9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019028">
        <property type="entry name" value="CBM_49"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09478">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035258181" description="Carbohydrate binding domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate binding" evidence="2">
        <location>
            <begin position="192"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="282" mass="31295" checksum="547FB08C0F0E476B" modified="2022-05-25" version="1">MKIQSLLISFLVLALAVQFSNGLAVDKYSTPCQQYFAYFRYVSQNTNYCYSVSIVGLKANGEIESFKGHFHFSGADLAYKTIQPMVNLNASSVACNSKNKVQPFNSNSGSTLGNVILSLDGSITFKNKTFPLSCSFNELSYAYFVMDNTIYTLTLRAEGSACQTLGFGEACNAAVPVVPPASVYKQPINVTFSTQFLYNWRVDTVRTYQWGITVTNNEPFTISSIDFYSPNANPIDLWEITKGGEPNMYYLSGNILPNQSRTFSLTTTTPEPTFVVKSFRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ66</accession>
    <name>A0A8J4PZ66_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine-threonine kinase receptor-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_003037</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the WD repeat STRAP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000090">
        <property type="protein sequence ID" value="KAF2075664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ66"/>
    <dbReference type="OrthoDB" id="408728at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032797">
        <property type="term" value="C:SMN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877:SF13">
        <property type="entry name" value="SERINE-THREONINE KINASE RECEPTOR-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="52"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="136"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2075664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="281" mass="30763" checksum="FBBD842E0DD32477" modified="2022-05-25" version="1">MRQPLICSGHSRPVSDLSYSNLGSDGCFIVSACLDGKPMLRDGTNGDWIGTFEGHKGAVWSSRFNSTASQALTASADYTVKLWDTLNGGEIVSIEHQSIVKTADFSNNNSRIVTGGHEKILRIFDLERPTDPLFQISGHQNTIKCATWSVFNDDIVLSGGADEVIRIWDLRSGTQVSLCAKSPISSMEFSRDRKYLVTTAGNEVTFWDASSFYPLKVYSLPFEVNCASLHPDNSKFIAGGSDFWVHVYDFNTGNEIEVNKGHHGPVNSCRFSPDGSSFASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZA2</accession>
    <name>A0A8J4PZA2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">S-(hydroxymethyl)glutathione dehydrogenase</fullName>
            <ecNumber evidence="11">1.1.1.284</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_002317</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 11">
            <text>S-(hydroxymethyl)glutathione + NAD(+) = S-formylglutathione + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19985"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58758"/>
            <dbReference type="EC" id="1.1.1.284"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>S-(hydroxymethyl)glutathione + NADP(+) = S-formylglutathione + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58758"/>
            <dbReference type="EC" id="1.1.1.284"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10736"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a secondary alcohol + NAD(+) = a ketone + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17087"/>
            <dbReference type="ChEBI" id="CHEBI:35681"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.284" evidence="11"/>
    <dbReference type="EMBL" id="AJWJ01000063">
        <property type="protein sequence ID" value="KAF2076360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZA2"/>
    <dbReference type="OrthoDB" id="417550at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051903">
        <property type="term" value="F:S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046294">
        <property type="term" value="P:formaldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08300">
        <property type="entry name" value="alcohol_DH_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000003">
        <property type="entry name" value="S-(hydroxymethyl)glutathione dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000001">
        <property type="entry name" value="S-(hydroxymethyl)glutathione dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014183">
        <property type="entry name" value="ADH_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02818">
        <property type="entry name" value="adh_III_F_hyde"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880:SF12">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE CLASS-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0520">NAD</keyword>
    <keyword evidence="5 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="13">
        <location>
            <begin position="33"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="12">
        <location>
            <begin position="203"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2076360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="376" mass="40613" checksum="AED70D2D7C8DD3B3" modified="2022-05-25" version="1">MTQGTTIKCKAAVAWEAKKPLVIEEIEVEPPKKGEVRVKVLYTGVCHTDSYTLDGHDPEGIFPCILGHEGGGIVESIGEGVESVKVGDHVIPLYIPECKQCKFCLSGKTNLCSVIRITQGKGLMPDNTSRFKCKGQTIYHFMGTSTFAEYTVLPEISVCVVRKDAPLDKVCLLGCGITTGFGAAQYTAKVEKNSTVAVFGLGAVGLSVVQGALDAGATRIIGIDTNTSKFEHGMKFGCTECINPKDFDKPIQQVLVEKTDGGVDYSFECIGNVNVMRAALECTHKGWGVSTIIGVAPSGAEISTRPFQLVTGRTWKGSAFGGVKGRSQLPDIVDKYMDGKLKVDEFVTFSYPLEEINEAFHVMHEGKSLRSVVHFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZA6</accession>
    <name>A0A8J4PZA6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000697</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000014">
        <property type="protein sequence ID" value="KAF2077973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZA6"/>
    <dbReference type="OrthoDB" id="191601at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008551">
        <property type="entry name" value="TANGO2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17985">
        <property type="entry name" value="SER/THR-RICH PROTEIN T10 IN DGCR REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17985:SF8">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05742">
        <property type="entry name" value="TANGO2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="388" mass="43938" checksum="0C98E75D38C26A12" modified="2022-05-25" version="1">MCIAFISFDQFSEFPLIILDNRDEKIDRPTISLSEWELGVGGAGEDGVSDQPKIRIFGGRDKLGGGTWFAISDRGKFCVLLNSYSKNELFGQLKPLVKITRGKIISDYLLLDVSPLDYINKHLESQRDSYLPFKIIIGDIKTKENYFYDSHHYLGSKPAIEQEPDKLFLASPSSSPIHIEPPLLVPGSSVDSDQDHIVSPLISPTLTSVNFNNSNNNKEKDSDNDKTTLAAKDLKSLTIKLSEQHQKMFGISNYPVHWNTFKVENGKKQFEKTLQTLFNENSNSNINELQQSSTTMFHSNNEKDKDDFIIDRLFQILINKETDSQPPAPFENINTASIFVNQYQSKYTGSTHATVSSSILLVDKNLNAKFVEWDHIHSSKHLVQFQIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZC9</accession>
    <name>A0A8J4PZC9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002902</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000086">
        <property type="protein sequence ID" value="KAF2075769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZC9"/>
    <dbReference type="OrthoDB" id="15717at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.90">
        <property type="entry name" value="CDGSH iron-sulfur domain, mitoNEET-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052950">
        <property type="entry name" value="CISD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042216">
        <property type="entry name" value="MitoNEET_CISD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46491">
        <property type="entry name" value="CDGSH IRON SULFUR DOMAIN PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46491:SF3">
        <property type="entry name" value="CDGSH IRON-SULFUR DOMAIN-CONTAINING PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="165" mass="18536" checksum="7E758AEDDDF7C9E4" modified="2022-05-25" version="1">MDDNDKVKETTDTCPPSTTDADKNSGCRCKCDDHGDDNNTEEVFITQSIDKQVTPSICKKCGLCKYVPLKIPMYAPYTLKGLDPNEQYYYCTCGLTTGQQPLCDQKSCIGTKFQPKPFVLGKKQTMFLLCGCRYTSNPPYCDAIHTRLPFNPTDPPCKCSKVDEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZT5</accession>
    <name>A0A8J4PZT5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_002183</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the crooked-neck family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000058">
        <property type="protein sequence ID" value="KAF2076505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZT5"/>
    <dbReference type="OrthoDB" id="360390at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055433">
        <property type="entry name" value="HAT_Syf1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17204">
        <property type="entry name" value="PRE-MRNA PROCESSING PROTEIN PRP39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17204:SF25">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23240">
        <property type="entry name" value="HAT_PRP39_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23233">
        <property type="entry name" value="HAT_Syf1_CNRKL1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="680"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="785"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="569"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="859"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="9"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="590"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="614"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="859"/>
            <end position="887"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2076505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="905" mass="103976" checksum="E23B18881EB815C9" modified="2022-05-25" version="1">MMANKDDQYHEDDEMEQLDGLDDGSSDSDDTSDDDSSSDDDENQHDTEHSSKAYEVDHLEIKLREDPYSFDKNIEFINALQGITKSNSPTSLERLRKARESFQSLFPLPEFVWVDWLSFEIKVSKTEQDYEYLDSLFEKAINDYISIKISIMYCSYKESIIFSKLSSGTLVIDSFSVPLVANQRDLFEKMIKKNGGDISEASKLWRRYIAFEKIVLSNLSDAKECAQSIKYINSLYLRLLSSTVVDLIDCFAEFKEWEHSKEIDDDYIEGIVEKSIQEVDARDPYEQAIATTDKQAAFPKWREYIEFEKKQTDNVRLTVAVYERAIKMYYSSIELWQGYLEYAQTNVSSVSQDILVNIHSRAVRNVYWSGDIWAGYMRVLERHSQSSETIHAVFERGLVAGLSSLQDCLVLYNALLDYQWRLVRAKSQKASTGKTIVDEQDIQSLQAIYQRACDYFAPLDAAILLDCQLYWANFQLESLGQWSEYVPTMETVLTYSFTQYLVWAQYIANTLIHATKEDARQLYQKAVLSVQDNAGKIWQDWLNFERQFGTLSQYEEALKQYHTATMKYLQQQQQHQTREQKGKPKAAKGKGQDKGAKANAAKEKPKRDRKRKSKKDDDGDDQDKANETNGNGKGGDVAMQVDKKPEQQQQEQEQEQEEEDKRKSKKIKTTNDDGGEKEICSIFVSSLPFETKEEEIQQLFGKYGEIKSIRLIRDKRGKSKGICFIDYSNTAEAKKSLELNGHMIDKFSLRVEYSKNNPPSVTKSTTTTTTNDDSSKIDYNNIEEKTVFINNLSPKITNELLEEYFTKNGILFSSVRVKNNKGARPFAYVDLVDKENLEKALSLNQKYFMGKSINVYVSKPPGSKSNNNSSSNSVDNSNSNPTTTSDVQFRKPTLLIPRGMLNKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0E9</accession>
    <name>A0A8J4Q0E9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001081</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000024">
        <property type="protein sequence ID" value="KAF2077618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0E9"/>
    <dbReference type="OrthoDB" id="18379at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20558">
        <property type="entry name" value="CYCLIN_ScPCL7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF69">
        <property type="entry name" value="CYCLIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="2">
        <location>
            <begin position="106"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="279"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="367" mass="42304" checksum="1BEFBFC5DFBD6DB3" modified="2022-05-25" version="1">MDHNSFYHQSNNTYSHSANYYQDRPTRNQISTQYPSQTPVTILSILKDKRIQLGEISNSWGENGDWLLRSLVMAINQLLSINDEFPNNYSHFLPEHMKLPMITIEGYISRIIKYSPCSKECFIIILMYIDRLIQKRNFIVNSYNIHRILITSVLIAAKYLDDIFYNNQFYSQVGGVTVKEINIMELDFLKLLSFDLSANSVVYSVYLSHFESYTQKIQTELNFKPNIESLPTPFHITEGYIKGFLKSNSKFIPEVEKCDLLIDYTSSSTTSLINNQAPNSINTNSSSNNNNNNNIVNNNNNSSNNNRNNNITFNQNNQQTTTTSTSNGQNQNNNNNNQNNNNYHHNINTKSPLQTFRFHQPQPNCVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0Z5</accession>
    <name>A0A8J4Q0Z5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000763</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000015">
        <property type="protein sequence ID" value="KAF2077963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0Z5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035196066" evidence="2">
        <location>
            <begin position="18"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="482"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="538"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="552"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="562" mass="64801" checksum="5CF287BC07526823" modified="2022-05-25" version="1">MIKVFTIFILLVPVIISQQWNAVPGNIKKQLDISYDIIFPRHSKAPISQFGFCFNPSQSLRTVQWGSERVEEKDKDGNPIKGPDGKPIMKIIYDWTVLIDGGKEPVRIRDEKAKFLEILPSFENSEFVIQHMMGDTKTDHFHSESIMLEAINTYKGLKDKCEKGGFYLFTKFMACSTCSGKLLEEILNKKQESINRPGKVSQQLDSFAAFKEAIPFYVFYSFQWDTDKYHYANLLRFQKSKIHLVRYGFNEWSTPNNKVTWDPKSIQERALETFTFYDVEKPMEKPKVLNNNVSLLNLFHSSIKNKKVRMILFLEKIWFPLCSTNCGLKWDFGCWKKVGMSIMTEYYLTKKDVDTLVAFKYYLDKEGEDYRASLKTVKISSTDPNKPDEAVPNNVRDNPERYSVPIGPLIQEGDTFQLDNGAQTQLLQLTITEKWCQGISKDVKQHTIFCHEIDSEVNLDFSENHITKLIKSVINASIDKSTTSEATKESHQTTSQKTSHHTTTHTTTAQNAQNAQITPKPKTMAEILFEAQAKKKIKKKKKAFKSMPYSYSKGNSWTWTDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q137</accession>
    <name>A0A8J4Q137_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001410</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000033">
        <property type="protein sequence ID" value="KAF2077285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q137"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000003">
        <property type="entry name" value="Rho guanine nucleotide exchange factor 10 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051092">
        <property type="entry name" value="FYVE_RhoGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673:SF92">
        <property type="entry name" value="DH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673">
        <property type="entry name" value="FACIOGENITAL DYSPLASIA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="3">
        <location>
            <begin position="335"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="129"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="700"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="731"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="261"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="501"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="135"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="169"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="735"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="756"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="783"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="803"/>
            <end position="820"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="951" mass="110543" checksum="ED3902188996679E" modified="2022-05-25" version="1">MQSIDNNNNNDKDKDKKSKSKSSSKKKDAGDGIVISNPTNFRQLSTPKNSNITNQQNISADEYIYDSNSNQNNVNYNNSNDLYNQDEYNQYESYNQDENYNHDQQLEQELNQYENDQYEYNQNYDQEQYYEEQQEQQQPQQLQQPYCPPIPTSKPRMSLQNALKSPPMSSNNNINNNSSDSSNNNNNNNNNNHNHNHRTSKVYFKVSNELLKGVKEWEEKKELYQQSIKKLLSSKSNELDKIFSHYLQEMKPILSQFQFDETMKKEILDKEEKRLEEVKQQLFGSKNASILVLENQIQIRKKEILMHNKITLLQKVVRGWLARKRYSKLKSDIIQRNKCAHEIIETERRYVNSLELIINVFLVPLQSTKKDLLSASEITTIFSNCTSLYGVHKELLESLEKRWEKWDQQQCLAECFINLSPYLKLYTQYINNFNNAISTLSECKKRDSKVKDFFFKDCKTNPLLDKRDFLDLNIQPVQRIPRYRLLLSDLLKHTPEGHKDYNNIKKALEEIQNLANNINESKRNAEGLEKIMLIQSSLTKNIELVQPYRRHIKDITILFDKKGRVKERHLFIFNDSILLCKKQSNILPSGIVESSGNHRYIPITHMSLYGAIILQVDDQVIKHKFAILGNQHLYFYAKSAVEQHELILLLKEIVKELDENNTSLKREEIDTLQRPLVRESSSYRESVDLSKILNLQKLANTNNNNNSNNSNNSHGISSNNLVRERNSTTFFSRTDSNSSLGSSGGNSNNGNDSPKARNSPTLHYSPPNSMKSQNHSSSPLSESINIQSIQQQQQYLPTPPSLQSPQSLNQPMNNNNNNNTIIQPQINLSIRELIDIYNEICNLQKEIFQSNNNGFNQSSAASLDKLNKTLERISTLKNQSLEILKQEEISSRLQEAITLGKDHIGAFTEWIKRSYTPQDPSGQYPKQLTINLMKWYNHLFTIWSSVLDYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q189</accession>
    <name>A0A8J4Q189_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NTF2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001834</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Has a role in nuclear-cytoplasmic transport of proteins and mRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000046">
        <property type="protein sequence ID" value="KAF2076867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q189"/>
    <dbReference type="OrthoDB" id="6507044at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00780">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000005">
        <property type="entry name" value="Nuclear transport factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045875">
        <property type="entry name" value="NTF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002075">
        <property type="entry name" value="NTF2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12612">
        <property type="entry name" value="NUCLEAR TRANSPORT FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02136">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NTF2" evidence="3">
        <location>
            <begin position="6"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2076867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="119" mass="13849" checksum="6C94C615461D3D23" modified="2022-05-25" version="1">MEPQQVAQAFTQHFYNIFDSDRRNLEQIYRPESVLSWEGKVYQGKDAIVKHLVNLPFKNIVRKIDMDFQPTLNGIMIFIVGTLAIDGEAKALKYAQTFNLVLHQGQYLLLNDFFRLALE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1E8</accession>
    <name>A0A8J4Q1E8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001646</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000041">
        <property type="protein sequence ID" value="KAF2077014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1E8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035240818" evidence="1">
        <location>
            <begin position="22"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="245" mass="27875" checksum="FA7FD77A8395E586" modified="2022-05-25" version="1">MNFIFLLSLLSLSTLVLKINCQEYLQQQPQQDNINANDYSKKIALTVLAINGIIEMNNGDIKKIYTISEWATSLNGAGVSLYSAYTNTSYFYNTRISNKTSELVKINNTIDDFTDLAGPVTTQFGNDSVVMNPYSFKYSLSLSKYQFTGQGNNSVVIKLSAIDVPNLSNSTYQFIKLKVIDNYIYGKLVIKAIIDGVSTPINNIPLDSDNKIIENNSSLRKKTVWYWDRYSNLYHICSIRYFDYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1L5</accession>
    <name>A0A8J4Q1L5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001126</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000025">
        <property type="protein sequence ID" value="KAF2077585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1L5"/>
    <dbReference type="OrthoDB" id="370698at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030119">
        <property type="term" value="C:AP-type membrane coat adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029392">
        <property type="entry name" value="AP-5_subunit_s1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16120">
        <property type="entry name" value="AP-5 COMPLEX SUBUNIT SIGMA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16120:SF0">
        <property type="entry name" value="AP-5 COMPLEX SUBUNIT SIGMA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15001">
        <property type="entry name" value="AP-5_subunit_s1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="133"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="264" mass="29861" checksum="8611BBB857ACC50C" modified="2022-05-25" version="1">MVYAILIHALDTPKGTQASIQQQQQQIGNEPVVFLSQFYTSEGNNQDFLKRQQSIVKRVIKDYTFKHQCENTKPDEKLFPDWAFAFAQRGADQSNSSKEGIFRISPLKKQPLACSSSSAAAASPLQSPPPQQQPSQQWPSDHLSSISTSITHSSRKTQVVPIDDPSQFISSPKYVIWRKICGVGFTVVCEEDENRLLLSNFLTMLAQLILDHFKLTSKNLTTEVFTRTDDILLILHNYLPNGQTLFISNSFSRQLKQNIQSQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1Q1</accession>
    <name>A0A8J4Q1Q1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Wbp11/ELF5/Saf1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000322</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000006">
        <property type="protein sequence ID" value="KAF2078338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1Q1"/>
    <dbReference type="OrthoDB" id="21340at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019007">
        <property type="entry name" value="Wbp11/ELF5/Saf1_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09429">
        <property type="entry name" value="Wbp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Wbp11/ELF5/Saf1 N-terminal" evidence="2">
        <location>
            <begin position="7"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="296" mass="33074" checksum="D48BC4D4782A9466" modified="2022-05-25" version="1">MGKNNLSSNPMDTYRKQVKKKEQEKQLAEKNKSFEKILNSQDPDVLLHELKKPIYNIDAAGKVKKKQLQSAYDALTKKNEPKQTNTTKVVKMDPALEEAIKKYGPPEESIYYDPVLNPYGIPPPGKPNIRKKPAATMPMIPPHHPQHYQQGMVPSPPPPFFKKQGILPFPPGMPPMMPPGFPGMPPMMPGMPPMMPPGFPGMPPMVPPPPPNTKHLPPPPIGMIPPPPPFIPGFLPKLGPLVHNNSNSNNSNSSNNSNNKPLENKNDTKEKEKEIEPTEEEVDEGLEEFMKEMKDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q206</accession>
    <name>A0A8J4Q206_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000350</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000006">
        <property type="protein sequence ID" value="KAF2078366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q206"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="258"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="405" mass="46813" checksum="8D3A25BB8FEF3514" modified="2022-05-25" version="1">MILAEQGPNLHDRFYSDGTIAIMKGSPYLVLNNEEKGKGVMKKKERIRERTRERVQIGLILMIALGCILLALLPFLLGTIETSTIVCGDVGVGRSLNIWSASGQKELYHQSFTLTGTQQINILRKESTSFIETSFNTFVEALGFSFRKKLIFVLNLESGRVEDQYLRIQLALTYLPLSTPYYLVYSRVSSKDLKIDFGKIHHQYLTIQPAGYLLTKEYDKQDEASKLLISKEIHAFIENAESFYSSNHFRGLSYRERLKLVQEKHTDIKERLEEIQQLEVPNPKDITSKSSKRHKAEIEKENKLIQEHLEKNPTLNSCKFESKVEEQPFEETSKHQPLNSFYKWRPVTVIRTTTEKGILRTTYARMVCEGKFGVITSQDWQETGKSRIVTFNQTRRPHYFGIAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q310</accession>
    <name>A0A8J4Q310_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000887</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000018">
        <property type="protein sequence ID" value="KAF2077842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="18551at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="72"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1400" mass="161745" checksum="3403473E55D1151C" modified="2022-05-25" version="1">MKRDTSWPDRVKSRNPDTKSTTLFSKWKRDNDDEVVKLNVYQDEVFLFKNENIPVDVRGLIFYIGTVYHPTEEDEEEEGDEEEEEKEQPEQQQLYNYDTLKSDFISIVSNLPSHISHLDFIIESDDMGYFLSFLPLYKEKEVAVPTSVTHLSVSGVDLSRDIHIPSSITHLTIDGITEDHNIPSTLQHLYIKGDFSTDVVLPQTITSLQIKKTGHRFSTHRLVCKQFKIKKSYKTYPELRDIYQQFDKLHYPPNYYSKMSSGFYRDQFSYEQQNSPIFDGTVMWLSKEPLKPDYIPIGVKRLFFGPLFNRPIAANCIPDSVEYIEFQGYDRSLSDIHFPSNVKTLILGNQFNQFIYGSDELPKSLTHLEIHIDTYDVFHWIYINGSLPINITHLRIKTTQPFRKRFSLPSSLKFLDIKAESHYWISIMDKEEYYKESLKLPEISTNCGLKHMTIRGDIAHNQKIPLPSSLTSLSFHKWWRRYDRKTTIEPVDPDHNFKSIVPSSLTILKCPNQSYIPLANMFTYLEYTIEPNIKKIVFKNSDKPISGSNLPMSFDVWTSVFSPNLVDKVDELHINEAAYSNDDYHEVRNRVSSYSAVKPNLNINVKKLNLTMIAIPVGYVPLSVTELGLFYARYPYSSPKLHPGSIPNSVKTLTSDCGINENLIPPSVEIFNCYGNLKFNIPPTIKVINLDFNENNLSYLLHQTKRDDTPPPKIINSYDGNNSDSFFVIWRNHYLKDQIMKLNYPSIFIPKDVDQVIEAQNRFINVTLIFENIKLFTKEMFPNNCKNIQGVQINFDNHYGEHQTDLLSILPRGITRLSIIHFKNIKIPTWITHLELVKSNYYYTDLKSLQIPPSVTHLTLLGFKPITKQIINNMVPSAVVCLGLDTAEIEKDSIPSTITSIQIKKCENIFLRCSFEEVIPITVKRISFSTQDFSTVQKFSEFVSSRLDNIEIYDKKKTISPKTKTVIWSENEPIFEGQIPNHISRIIFGKHFDQPIVENSIPNSVKEIYFGEKFSQSLSNVVLPPSITSITLGGYYRKGLNFDMLPSSVKYLAFYDNGIHKIKTIPKTVSHICFFSRNSNTSFPTPSHVEHIKVVDSVKIRPYTDKSFINLSPVVRSIQIEGTIKFIDKIETLEQDEEELIKPFGQDLQVNLVSKSDCKLNDFLKPGSLISNIKSIEFPNYYQQIILPGALPESVTKVNLPLDFTRQFAPPPFLKILHLGHKLNYPIQLPNTIEQLTISKSFSFPIGFFPQNLKIIIFNCEQFEEKFEAGIFPDSVEEIHFHACYPFAFRENTLPRNLKALSCDYYCDLFEFLPKSIQELNFITKTYQEGLYSEISAKDLPPSLTKLTLARKQPIQDLEYLPPTIKYLKLGEYFKGKIPNTVETLKLPFYHKTPLAYIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3C1</accession>
    <name>A0A8J4Q3C1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ABC transporter B family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_000565</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000011">
        <property type="protein sequence ID" value="KAF2078094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3C1"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18574">
        <property type="entry name" value="ABC_6TM_ABCB8_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000215">
        <property type="entry name" value="ABC transporter B family member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000916">
        <property type="entry name" value="ABC transporter B family member 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF1">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B MEMBER 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002773">
        <property type="entry name" value="ABC_prm/ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="252"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="353"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="216"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="527"/>
            <end position="763"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2078094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="770" mass="84438" checksum="4238289EA1B1F1BB" modified="2022-05-25" version="1">MMLSLITRNSKSIVGNKKLYSHGISSINKPNLANGGEIGKKRIDLNLSNNNDNNKNSNIKLIGQRLNNNNNHIHKPSIPTSSILSIKNSLINGIIGNSNNNNSIRLFSTTSSSNNSNNNSSNNNNNYNFKSSFKLNFNVIDGVIIVGCTYFILKFYNDRIGVVFCEETNTIPSKEIFIPFKEDGDDEQEVGERHKPLSPFQLFWKTIGNDIWMFCFGVVTAFLSSWIGLQIPKVFGKLIDSTKSGSSLTQPALHAVAILVIQASLNFLYSTLISIACERYSAKLRNTLFESMLEQEIAFYDKNSTGDLLNRISSDVQLVRSALKHSVSLGVKSIGQIVGGTISLFLISPKLSLGMLTILPAMVGVGTFYAGWLKSLSLRSQAAQAQSTSVAEEAMSNIRTVVAFSNQEYESQRFAEKNKKSLSLATESGIQIGIFQGVTSMALSSISLLVYWYGGTLVSAGEMTSGQLTSFIIHTMNMQNSFSQLSILFTQIMSAMGGMTRITELINKTPAIESGVGIKRNTVVGDIQFRDVQFSYPARPHIQVLKGLNLDIKQGQVVALAGPSGGGKSTIASLLERFYDVDGGEILIDGVPIKKINAKWLRNHIGIVSQEPSLFATTIIENLRYGCPEATEQQVMEAAKLANAHQFIESFPLGYNTIVGERGIQLSGGQKQRIAIARAILKNPQIIILDEATSALDSESEHLVQEALQNLMKGRTTLIIAHRLSTVQNADAIAVVKHGVIVEYGSHSQLMAKKQLYYQLVQRQLSLKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3I2</accession>
    <name>A0A8J4Q3I2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Clu domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000633</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000012">
        <property type="protein sequence ID" value="KAF2078082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="19471at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033646">
        <property type="entry name" value="CLU-central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025697">
        <property type="entry name" value="CLU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027523">
        <property type="entry name" value="CLU_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601:SF11">
        <property type="entry name" value="CLU DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT EIF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13236">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12807">
        <property type="entry name" value="eIF3_p135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51823">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Clu" evidence="2">
        <location>
            <begin position="359"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="86"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="131"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="301"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1527" mass="172887" checksum="2411F90896B47D1F" modified="2022-05-25" version="1">MTNKFNDIMAYWDGKDTKKHSHPPRPSRKRAPVNNNHNNNQQQQQVVPDTKVDLHPPVSLEKVSVSLNKDSDKDHVHTTDDDLGSTTTTTTTTTTTTTTDNNDSHPPISLEKESDKQQPSEETKVDEASDNNNNNNNNNNNNVVSSGYSNFVVGDEIKTSRYENVNNIDTSSLGSSTNTNNYSQEAVAGNYSAYSHHGGSTGYSVPSHYSASNYGSSYRGNSYTVYSDEEGDDDDLESQDDETFGTGKAKGVAKHPYFECDISEADLLKLVDLVDTISDPPPVNDGTLSLRGAGDKDQEPEQTQPQPHQYSSSYLDTSSATSGTTSDTNSGYGYAGNNPYISGASQLNNYSHAKFLTEKGIDGGGKKHKDDPNSKYDDLTSWNTEFQRVIEMDDCEEKFERLSSLEQDFVYAAETYGRIIISEHFLPHENRTIKPVSVGGIAGGEKYIVQGILFKFAVENETFGLYGNDENAMKTASHEINGCTSFLNCGIKGLHVPLMAYIDYRGFRLMAMSLLPISKKSLIYGSSDAGQTVHTSNTIFNHLFSSAAKIVNLKPHLVQDMSGDLKTIHGPIDIEGHIGTDNRFYLLDFARLSPPEPPTHRGAYLYRLLRLELVRKYAQPLCSDAYSPMINIGIEGHNFEVNEAYQHLVDTIVPNFAKTFNDENKSKSDIDNMFMEISKLLHIDGINVRLLGKVRSHLTFGYTRICSLIECIVRTLKSLTRELLRREMKRSHLPSDYPYRKTVLNFLNLIFGDTYKTQDFWTNILQPKLEKKFQDVFVMSTVVEETIVDENNPENSRVEKKIVSTIEEDPFKVILTSNDYRYNLFKRFVNSIGVVMRDEAKTDFKNFSTGFTFVDPDIVEMKTLITRLNIIDYADGMKLYYKSMKENVDPKTIKRLLSVSKNRLEKSLHSMSTNYSAYFQLGKVIHQMAKVQDGETQQATLYYEAAERFKTLESHRIDPDLLFISKLSRAKSLIARYAIHRSYEDIPVEIQEIFDTLLPLCTGLNRSKALAVYIKFLCIKHLKSDSQQEYEEIEKLYNEIFEKDPSNSKAKINYAMFLFESRKNHNLFSTDKFSDLIIQGLSGSVKNIEKIRPFIEKSPWKLFACSRSILKLQQVVKTSIENANKKGFLTIPNDVSIEVGDRVYFENIQFDKLIINNLDISSTALNMISFFADTVKYINFANSKLLLHSEVLRSIPLVSNLCSIDFSNADINDESLVPIITPLMKKIRLYNIENISNTTLESIARQCADLEVLDLGQCRGMDGSIELLIAQCPKIRKLACPADISEALIYSCAENLRQLYALDLYKCDSAKPNVCELVFTKSQSLKKVRGHAGFPYFYITAPDYVTMRLNKQPYHDTQMPGTLNSQKTQLYYIKIGHNHLNHKVFSLFMNNSHPLVKFRHQPNLLADGFYKVFHAVRADKPMPQLTIYANNANNRWDVSEHITRMPLNGSTFQVQINYQATAYNSRVLLQASHGGQTFSNTLYRSDTQDQVLFLTNAKSMKMTIDCDFKKSITMIPFILSLCLAYEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q404</accession>
    <name>A0A8J4Q404_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Coiled-coil domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000339</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CWC16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000006">
        <property type="protein sequence ID" value="KAF2078355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q404"/>
    <dbReference type="OrthoDB" id="360327at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007590">
        <property type="entry name" value="Saf4/Yju2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12111">
        <property type="entry name" value="SPLICING FACTOR YJU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12111:SF2">
        <property type="entry name" value="SPLICING FACTOR YJU2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04502">
        <property type="entry name" value="Saf4_Yju2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="121"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="283"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="121"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="139"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="348" mass="40152" checksum="F87F8775A1A7E245" modified="2022-05-25" version="1">MSDRKATNKYYPPDWDPSKGSINTYRGQHHLRDRARKLDQGILIVRFEMPFNVFCLSCNNHIGVGVRYNAEKKSVGKYFTTTIFSFRMKCHLCSNQLEIETDPQNRDFKIISGLKKRSTESDTNTISASTTDDQEIIDDNSNNNYNESNNSEYDSSLIKFKDEKEAELLENNTFYRLEYKNKDIEKGKRLAPNLQNLYYLMGDRSFNDYNLSSELRKNFRDTKKAEQKEIKENESKGIHIKLVPEHNDDIVESKSINFDKQHLKRKAEQDKLIKRETIQNSSIFNNNSSSSNNNSLPTTTSKISKTKSSTTTTNTKLELLKKKAKIDPSIFKSSSSTINTAFSSSLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4A1</accession>
    <name>A0A8J4Q4A1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">separase</fullName>
            <ecNumber evidence="2">3.4.22.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_000735</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>All bonds known to be hydrolyzed by this endopeptidase have arginine in P1 and an acidic residue in P4. P6 is often occupied by an acidic residue or by a hydroxy-amino-acid residue, the phosphorylation of which enhances cleavage.</text>
            <dbReference type="EC" id="3.4.22.49"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.49" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000015">
        <property type="protein sequence ID" value="KAF2077934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10255632at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051307">
        <property type="term" value="P:meiotic chromosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005314">
        <property type="entry name" value="Peptidase_C50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030397">
        <property type="entry name" value="SEPARIN_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12792">
        <property type="entry name" value="EXTRA SPINDLE POLES 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12792:SF0">
        <property type="entry name" value="SEPARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03568">
        <property type="entry name" value="Separin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51700">
        <property type="entry name" value="SEPARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase C50" evidence="6">
        <location>
            <begin position="2013"/>
            <end position="2109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1670"/>
            <end position="1717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1682"/>
            <end position="1716"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2077934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="2201" mass="253102" checksum="BBCA9E5D999D7011" modified="2022-05-25" version="1">MVSLSVDVESTFKIFEDKNEFLSLLQSKSNIPDIELEWKDKFNNLSLELIKQNDSKTLLTISDRLAKVLTEYLGSKTLTYKDGVSVLVSSLLVVLINMAKYVNTQNSTTPHKKWLLVGGILIDNKLYSKAFEAMSMFIELLSALKSKHPNNIDYFEKTCIHAMFLKMQCFEDSNIFNNSETSERMLDEFIKNYNSWFSSRFELADKPNMYHEAFFIKFYNRCAFFLSKIGMNDSQWPYSIQFKDLLFYHYNFVTWNSLAKSDSPIKFFDRLLIFLLILQKKNYYNDKNCWKMLNDIVDMIKVQVIPQMLKSNPEDPLYILKVDTTFIDCLCTISTFAIDSNQYTIAKELIEIVNQYLKKKKESNETSNFYQCELVIGQLHCWIQQCYYLLHQLTNTIENNQSPDTTDLEKELYKYLLTTHQLTEKAFVNSQFTIDAQNSIESKFSSIKFIGHTLCLVDQFVQLYIKFTKNNGEEKKSLKLEICQQIETFNEYYTLIPSVVNLKWLKAFDTLNPTKKFYTNKHVQGSLEEFIKNYSLCIYSLFKRMNGKLTDSFKTKLVETNSVVCGVVLNYTQGIEEFASYLYNLGTYFLKLIKPPDYSLAKGYIESSIQIINRILTLRKPPTEQTLHHMEMLSNCYFYASYIRLKVQDKSLEGLKKAHSLAIESIKCYFKGKTNFNQVLYESISGGKSPDTRSGAAAKQQYQQQRPFQEILRNYSLTLLHLFNTNLAQIEDFNQQQLGSHLSPLDGIKLINDIITIGDESFTIEFKIQVYDMMLLNFSQFIGGGEKNAHQLLLLQIDRFLNDLPSATYPILHARYLMERGKILRFYNPNKENIENHFQDSVDMIKNNQLSLDQPFHSAIYNELAKLHFWKAITHLELYQNQCKDILSSIRQNEKNSSLDYFDSSESELFKYSSKANSSLSISHFLRSSDSNRFRSPVGSSSSPITKELGLSISLWCKLLDSLEKPVLSAGKGTSGTTANYSVLQNNLKYFISPHTTLSILYNIADIYSFEGDYNNSIFVLKLVIALIKCLFSPHSMTFLSETFKCFNQISSSYLELNNTTQCLYYLQLSQTLLNSFQPILSTDRFDSYQMIFYVYLSEYKYFVNPNDNQLCEDIKKLYNQFIKQMETNNELVDISAFMKISKITSNIFISATDSAMAFKVADKLIDCIFSTMPLESVDLLKEAASGSKKQKQFNNGSSNSSVTITTSKWASLKICLDSLLHISSIYELRGRPKEAQYYYERGLLIGIIYGSLKITCEFLVEIGELFYNKHNFIDSKINLELVGFLLNRHQSNQQQQQSPYCLKVCLLLSNMLLGDLYRKQGLVSKSCQYYNKTLEIIQDKNNTSSLIDKFSNSLSIQESNNDSTPKEARLMKLLSSKSINVNNNNNNNNVGGGQKLKEFTIVETIDHLVNQPINPPAKDSNEGNQKSYRLLLAYEPRIQGKLAKLQILEKKYESAIDCLDRLIHQNTTSFKPNDITLTILEFHLGKAYFYNVDENVHEKIWSFDNYTSNTTSSKPHPNIASARELFLRAFQRIGKFNLIKLNASICKYICLSTGTQNPFVGTHFLNLAIGIKSRHDMQTIIQHHHQNNNQDKKSLAMRQSIFSWNGGVDLPSNLTDSSFTNQLKKLYSTELPSQWISCNIAHEGEYLILCRLSNNQVPILSRVKIPLIDNNQNDPEHENNGDDDDEEMEMEMEMEEDEEVEEDEDDNNNDINQDDGETKMTTISFSILDKIKGGLKTIEIENLLNNKENSPNFSNTEDSKKAWFYRRKVLEFEMKNILNSIDQLIGSSKHLLIGPLMNPNVIAMVQKSKQNILQELSKYSFPKNQPCSNSTNSKKTPATISPTFNINLLDHLFTSLPFLSFTTRIEMIIELLGYSKLDAKTQINNIVLKNEKTGVYDTQSTFDNHPNQGEIEFVLQMFIDEFINCFNTNSTGVISNSKIPKTLETCAKLYLSQKRMPVVFIIDKVLQSFPIETLEPFSQSSTYRVPSFAFQRHLTLDSTTGHCKQEREYVDTKSLFYIINPSGGLSETQDYFIPLFNKHFPSWAGIAGQIPTKPQYQNALEDNEIFFYMGHGSGEQYFRGDRIQKLSKCGVSILMGCKSGHLEEQGEFEPTGIILDFLLAGSKAVIGNIFDVPTSDCDRLTRSFIKTWFLNEYDPKDVNSINNQDMDISYALAIARKSCEWKYLVGGSCISYGIPIYLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4U1</accession>
    <name>A0A8J4Q4U1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004748</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000173">
        <property type="protein sequence ID" value="KAF2073961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4U1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34003">
        <property type="entry name" value="BLL2395 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34003:SF2">
        <property type="entry name" value="SNOAL-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="13658" checksum="D43E3A8AF0E2266B" modified="2022-05-25" version="1">MTTTDIRKNLNSILKAIQENRILEAFEKYYHNDIIIYEKGVNRVGKQENRTFEQGLASHTIVSDLKVLTLLVDENCSAYECIITFSTDQDTNITKSHYWSFQKWDQGLIVKEEILNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q581</accession>
    <name>A0A8J4Q581_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000176</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000003">
        <property type="protein sequence ID" value="KAF2078552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q581"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="276"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="320"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="362" mass="39608" checksum="98F85C3C5B9201E3" modified="2022-05-25" version="1">MNPLSGYSKYIRAGGNDENEQQSKRTISNLSISNDEERDYSQDDSNDSSFSSISNTGAELYREFQQAKQHPSSLLGLSKQTGSKSGSTLLNKNRNDNNSTNTESEDNGQEQQNNSFSDIGATGSGLLEEIQQIRSNPSSIGSHLKQHQTTTHSKPTPRTTTTTATTAPRSNDFNQDEEEQEEPKESEDSLFTKGSELIHGVQQTKQNPLSLSGLTKSNPNKPHQTSTGTNVDGEIRNNNNNQEPEGENEETTLSSIGTTGAHFFSQIQQAKQNPTSLLGSKSSNQKSSTMSTVTHNNSNNNNNVNNNNNNEEEQGERRNNSNQATSNSSEELSLSSIGNTGTQLFHQLQDAKKNPRGLFGFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q6A6</accession>
    <name>A0A8J4Q6A6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nucleolar complex protein 3 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_003880</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000128">
        <property type="protein sequence ID" value="KAF2074816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q6A6"/>
    <dbReference type="OrthoDB" id="10263597at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011501">
        <property type="entry name" value="Noc3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016903">
        <property type="entry name" value="Nucleolar_cplx-assoc_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14428">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14428:SF5">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07540">
        <property type="entry name" value="NOC3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleolar complex-associated protein 3 N-terminal" evidence="8">
        <location>
            <begin position="294"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="CCAAT-binding factor" evidence="7">
        <location>
            <begin position="659"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="91"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="189"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="939"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="556"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="189"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="242"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="961"/>
            <end position="984"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2074816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1000" mass="113811" checksum="EDDE3CD6E0A52D13" modified="2022-05-25" version="1">MAPTTITKGKGNVPGKKRELNRQKQELLTIKELTKFDILFREGEKDRNAVDKTVVAGQDELESDDDDFFTNSSFANSNISDASFRKLSLKKQMEKEGVDPSSLLKRKSFHSDSEDDQESEEDSDDDADIENAIEDQDIIRESQWDNQSVVKRKLPLKAYDGKVKTVTEIISAKDYKQGQEIQEQKKEIFKQEVQKDRQAKNDLKKKLKEQRQPNNNEISKQVEKNLQKAQPASKKQKQDKQDSDDEDFDIQDDELDLENRDLSDLEYDDDEVDQPHIKSEGEVFLEHQQLIDNAKNDLAKISSKVITNPELNIQSLKEIFSICLKTKDMTIKKYSILSLCALFKDIIPGYRISKDLQEQSTPSNASKKDAAKLSKDMKKVREYEKKLLKYYQNYLVLIENSINNILALLQHKRPSGGGLGFFKINGGYTGRDLKSLLQCIIKSVTILLVSHPHFNFRTNLVTITARFTVFKEDDIAMMALESVKSLFLNDSTGGETSLEMVRCLANVAKSAGYIIDPKVIRVFIAIHLTDVVEKINPFGAATDDTWKVDKKDRKHLTRTEKKKRKEDKALDKEMKEAEAEYSMKEQRYLQTEILKSIFILYFRIIKKAPQSPALSSVLEGLAKFSHLISVDFLGDLLKVLSDLMENGITSIKNALNTNITAFKTIKLHGNTLNVDLKDYYVKVYSLLTDMVLPKENANVETALDAINMMFYDRKQTSIERVAAYVKRLSTVSLFLPPHAALSVVTLCQQLFVTYPKAQRLLETDHTYAGGEYVFDTLDPDHCNPFASTVWELTLLFNHWHPKFEPLAKRFLAMNEDTINKQLTKERSPLEVFRAYDNTKGGFNPAIQTPKEHPLEAKLKKLKKSYKNKEVTVFIQPQKQYEKSSFLKDMERSVADKKVFDSSLSFAGYYHEINEYNTQMKLHKKIQSLQSIKQKLDSKQKELNSKVSQKPVSPVATAAPKLNSSKPAATPQSKPAASKPATTPKSKPAASKPVATAAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4QBB7</accession>
    <name>A0A8J4QBB7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Signal recognition particle subunit SRP72</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_000298</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SRP72 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000005">
        <property type="protein sequence ID" value="KAF2078431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4QBB7"/>
    <dbReference type="OrthoDB" id="5421607at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013699">
        <property type="entry name" value="Signal_recog_part_SRP72_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026270">
        <property type="entry name" value="SRP72"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031545">
        <property type="entry name" value="SRP72_TPR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14094">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14094:SF9">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08492">
        <property type="entry name" value="SRP72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17004">
        <property type="entry name" value="SRP_TPR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7" id="KW-0733">Signal recognition particle</keyword>
    <keyword evidence="9" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="456"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="Signal recognition particle SRP72 subunit RNA-binding" evidence="11">
        <location>
            <begin position="553"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="540"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="543"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="594"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2078431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="778" mass="88567" checksum="CE88D2760D4D4700" modified="2022-05-25" version="1">MSKETVSLDQLFKDLDENINNGQYKKAIRVCNKILGINNKDVEAFQCKVIACMQLGNYQEALDCFKTNPTQIESMRFEYAYCLYSIKKYQEALKEIEKSSKENRILELEAQIYYKLENPQKTLSIYESLLGKPGYADSVEFITNLCATYIEANEIQKCQEFLTKNKQFLAKAYELAFNAACLSIIKQDYKTAETQLKLAKKVCSDSLKRDGYTEEEIKKEMTAIDVQLAYCQQITNNLESALEIYQNIVDYKIEDDSASLVALNNIASLRDAEKDSATILESIKNLLSEPQERLLNGRQKKTIGFNLAILLVNLRKVGQFEELVKSLKSKNAADQISTPEQINHFTQELDIISASLMIKEKKWKDAEQLLKSSKTLKSKLLLAQLYLLDGNHIEKTISVLENLESQYKSKPGVIATLVALYEKSGDLDKSIQYLDQMIGSLESKSKKSEQEQESYLNLLRISGNFKMNHQKHKEAAETYEKILQVNPNDLIALPSYIVATSHYDPTLAQKYESKLPNIKFESKVDVDLIEKYGLAYDKKESESNTTLITDKKPSTTTTAAVGEKKPKKKLPKNYKPGDPIDPDRWLPKWQRSSNKNKGKGKKSTIGKGPQGVTSAAQLESLFVKESSEPKVSNTVTVKADKPRNIHKKKTLGLLCINIITLNTLIVHGKSQSDIEVVSLFLKEKCNTVSLGLLKEQDFVEIFCDFIDHIISHDIDSVIYFYKQSTDLVPLGMAVEDAIRQKYYNKEERPHQLVDTFLKNLEINTQSYLLSKRRQKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQA1</accession>
    <name>A0A8J4UQA1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_008266</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000495">
        <property type="protein sequence ID" value="KAF2070411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQA1"/>
    <dbReference type="OrthoDB" id="6130531at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035267146" description="EGF-like domain-containing protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="5">
        <location>
            <begin position="415"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="4">
        <location>
            <begin position="641"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="3">
        <location>
            <begin position="779"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1023"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1033"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2070411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1093" mass="120695" checksum="34560C3E15A8A58D" modified="2022-05-25" version="1" fragment="single">MNKRYLYLFLFVLVLVFNIQTSDALKATVIGFYKGPDTYADNLNKCGARDIWIQLTELSGTISNIVTDSPAVTLSLLPALYENSTMVTYKGSIEFTATPNIITVNLQVSTTDVTTGVVLLTEDNQQFQFQCLARPQSPTQATFIRPLQMDVSTYGYTSKFIGYFSLDPVFTKGIPDYQCQATDYSTCTVDYIGNGIYLAQFDLVSTYTGDLTLNSGRFVFQIGGFQMQFDNPTSTTLYKNLLISQPTIENVVSGQMSGGVFPFVSNQDIMPLITGPFRALKGNFKNLSLFFRYSGSQVRYMSNSLFSSTSSLPTPTPLQLTNTWSQSQTGVNLQIDTMYYAICGFSTNKKYYFSSDSNQKVDTSFPFGLKSVTNNIPDYKISTLFSPYQFQYRFMYGVNNAGFSRGYLISGYNNSDTTASKLKSLNVFPLNNTHSVFSVELSDDISGVGDIFVLLSNIGESIHLNQHALVRGTLLDGTFEKILPIQKYTNFQLTVSDRSGNAFNYGEDSLYLLNRVGKMLYKFYEFDITHLQFQKSSVDVSVSSVTNTLYLNFTLPAGVDRKFTSVQFKPILTPNKNVPTVYSGIYDQQTKLYVINFLIPAKTFTSELLYLIYINGIVVPSTSYLSKFESQSRLYITSSEFDQMFPIITSVSVSHSQEVDYSVSQVSISWTIKVSDENFVKKFIVQIASSYDLEGFTSTWDGSPSPSVEYVVTKQLDSTVLCKDQEYYIKYIYTEDSLGNFGETFRDVNLDIHPFYKIEPAYPDSIKVKCTPSISRAPPVLTTATVNQKLNVTTKVPTAIVNFSVSGNRGPSLPSCYFHSYPMDFLSTKAVLMSEDTLNNIIKYSCTIDLPYRYGPLVAVSIYGIVDSLGQVIGYPTSDMPATGKEFTSIASTYQGPVIDYVWRDGDKIGVTGTQLYQGEIFVYVDDVASTKPLTIETATGIAIIAGGFTYLANSTVYVSVFNPDLSEESNRLLLIKGTPSVTPSPSPSPFCNSDCGEPLGYGKCENGVCVCNPPHIGLDCKSIDDNTTPNPSSSPSPTPTPSPTPIACKSDCGEPQDYGKCVNGACVCNPLHSGIDCKAKTDNTTVITPNPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQY2</accession>
    <name>A0A8J4UQY2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein arginine methyltransferase NDUFAF7</fullName>
            <ecNumber evidence="7">2.1.1.320</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_007339</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Arginine methyltransferase involved in the assembly or stability of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)'-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48108"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:88221"/>
            <dbReference type="EC" id="2.1.1.320"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NDUFAF7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.320" evidence="7"/>
    <dbReference type="EMBL" id="AJWJ01000385">
        <property type="protein sequence ID" value="KAF2071341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQY2"/>
    <dbReference type="OrthoDB" id="17415at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035243">
        <property type="term" value="F:protein-arginine omega-N symmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003788">
        <property type="entry name" value="NDUFAF7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038375">
        <property type="entry name" value="NDUFAF7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049:SF5">
        <property type="entry name" value="PROTEIN ARGININE METHYLTRANSFERASE NDUFAF7 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049">
        <property type="entry name" value="PROTEIN ARGININE METHYLTRANSFERASE NDUFAF7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02636">
        <property type="entry name" value="Methyltransf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2071341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="484" mass="56622" checksum="45D2F24B2D0E8E65" modified="2022-05-25" version="1">MILQKSLQIKKNNVFKTLSRYYSISSSSSSSSSASNKLSNDLQHPILDKLKSKETIKIDDKQRNKKNSNENHLFKAKTQLMRDFIQDALYNPKSGYFTTKEVIIPENVQVKPLNSLANFKEYNNYLHDLYGQLEHAWLTPVELFKPYYSRAISRYIVEKYSQQQHPDTPLKIYEIGAGSGTNAVSILDFIKDNHPQIYENMEYTIIEISKLLAEKQVQRIKNKHPNVKININNTSVFNWTKTMEEQECFVVLTEVIDNLPHDKITHSFNGSFETVIHSTLENSLSKNQKHLIREELSKINDPVIIEYLEAEKIWSGKSDQDKSKLKQKLDNLLSMFKEDESVWVPTVCFKLFQILAKFFPRHHLVLADFDYIPSIVKGKNSPTVQHKQRVKDQTQVKYESIDLDDVTLEVGSCDIFFPYEWDQLYKMYCYTNKDRIGFDLANVKTFKHKDFIKQYGQQDLHLTETKSKYNPILEDYKNMSFLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4URA5</accession>
    <name>A0A8J4URA5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006808</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000330">
        <property type="protein sequence ID" value="KAF2071881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4URA5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="68" mass="7446" checksum="86F62DF56F4B0985" modified="2022-05-25" version="1">MALLKNLIFMGTLSNNPSKNNLSLSFSNSNSMNSFESKNLNCDTLKRSSGVLCVLENQPTPMNGYPAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4USE2</accession>
    <name>A0A8J4USE2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_005166</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Ala) + L-alanine + ATP = L-alanyl-tRNA(Ala) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Monomer.</text>
    </comment>
    <comment type="domain">
        <text evidence="11">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Alax-L subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000199">
        <property type="protein sequence ID" value="KAF2073516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4USE2"/>
    <dbReference type="OrthoDB" id="2423964at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00673">
        <property type="entry name" value="AlaRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000004">
        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000004">
        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.54.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.550">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_B">
        <property type="entry name" value="Ala_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050058">
        <property type="entry name" value="Ala-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023033">
        <property type="entry name" value="Ala_tRNA_ligase_euk/bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777:SF9">
        <property type="entry name" value="ALANINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777">
        <property type="entry name" value="ALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 11" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alanyl-transfer RNA synthetases family profile" evidence="13">
        <location>
            <begin position="24"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="448"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="601"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="605"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="703"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="707"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2073516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="910" mass="102501" checksum="DBDC8116D1D3F5E5" modified="2022-05-25" version="1">MILKPLSHQHIISHNVRRYSTSTFTTKDVRNTFLNYFEKNQHKQLPSSSLLPHNDPTLLFTNAGMVQFKNQFLGLQESKHKMVTTSQKCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGGYNSFKRDAIHHAWNLLTKEFNLPPSRLAISVLEGDEESADIWRKEIGLPNEKIMYLGKEDNFWSMGDGPGPCGPCSEIFWDHGPDKLVDGERYLEIWNLVFMQYFRNDKGVLEKLATPCIDTGMGLERMTSVLQSKATNYDIDLFQNLINGVKQQIHSNPTQSALVGKENPERVETALRVVIDHLRSMAFLISDGIIPFNVGRGYVLRKIIRRALSYGKILGFNQPFLYQLVPLLIVEMGDTYPQLQERFKEIQNVIYNEELTFYTAIQRGIPYLEDMIDKKSLNEESLFDLYQTYGLPLEMSEVKAKQNNISINMEQVNKLIEDTRKKSRSTWKSSGSGGSSSNNATVPDQVLRWKNDNIEPDFIGYQSTLYDNSTIQRSFINTEEGDNLIYISLDKCPFYGNSGGQVGDTGFIKSIVSGNTYPVVNTIKPYDKGLVIVCEWDPTKQIVSDVVKDLQSNAQVFCQVDKSSRKQTAIHHTATHLLHAALKSVLSGSVVQAGSYVGPDNLRFDFTYNQKLTPKNIIDIESWVNRAIASDISVVTNEMDYQEASKTDAVQLFSEKYTNKVRVVSLEGHSKELCGGTHVERSSDLHHFKITSESSVAAGTRRIEAVAGLAATQWFENHFRLLSSISTKLDVPIQNFEKTFDKLVATSKAQEKEILDLQLKLATQSTRSQSGVTADGIKVFIHMIDCDDKKILATITSNLSKQHADHVHLTLTKTGKLVCCCNSQGHKADTIIKQLLQHVGCSSGKGGGGNQIAHAFFTNFTQNHHNSILKWANCKEEFINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UU68</accession>
    <name>A0A8J4UU68_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010157</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000950">
        <property type="protein sequence ID" value="KAF2068518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UU68"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF180">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="352" mass="39143" checksum="336E47563052AAA6" modified="2022-05-25" version="1">MPPSVTAFKSNFPTFPVYPFSTSNLVSIRMAHENKFLRISFNIHPGTFPETLKFLDLDSYIGEIHPNSLPASLTDLRLSYATNFSVTVLSLPNTITKLYIKQSPKSKNSKLNVLLPSSLTDLQLMDSPQLVECNPLPAGLKKLQLSNSDLSPTNIVPLGVTDLSYSFNDYKALYPHHLPTSLIHLHLHSHLQTISLPHLRTLDHLKELQMGSSLDLTYLPPNLTKLSVEIPLSDTTNIQLPNTITDLTLWMTELTLFGDIHPDFISVSDSKLPLLQTLTIVGNESNSLTIDNLDFLPNSVTSIINLPMITSNTFTLPPLLNTLEISSSNYKHNFTDKGISLPSHVKFLNVHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UU96</accession>
    <name>A0A8J4UU96_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HD/PDEase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010098</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000922">
        <property type="protein sequence ID" value="KAF2068578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UU96"/>
    <dbReference type="OrthoDB" id="16547at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33594:SF1">
        <property type="entry name" value="HD_PDEASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33594">
        <property type="entry name" value="SUPERFAMILY HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G03035)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD/PDEase" evidence="1">
        <location>
            <begin position="17"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="229" mass="26377" checksum="C186CC7BCBFF1947" modified="2022-05-25" version="1">MILETIDFVKKELKNLDSSHDWNHIERVWKLALYIAKNEDYKGDLELLQLAAILHDVKDHKYAAKDGTADTATPESAITLFLSSLNYPKDSIAKVCLIVQQISFKNELGNLNQEFLIESKIVQDADRLDAIGAVGISRCFSFGAVHHAKFYDELSSYQSYSDRETKEISQEEYIQKFKSKGSSIDHFYDKLFKLKDMMKTQTGVKLAEQRNQFMISFIKQFDSEIELLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVQ2</accession>
    <name>A0A8J4UVQ2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Major vault protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_009670</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">The vault ribonucleoprotein particle is a huge (400 A x 670 A) cage structure of 12.9 MDa. It consists of a dimer of half-vaults, with each half-vault comprising 39 identical major vault protein (MVP) chains. Dictyostelium is one of the few organisms in which the major component is actually two proteins (alpha and beta).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000772">
        <property type="protein sequence ID" value="KAF2069013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVQ2"/>
    <dbReference type="OrthoDB" id="6125719at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08825">
        <property type="entry name" value="MVP_shoulder"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.560:FF:000002">
        <property type="entry name" value="Major vault protein-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.570:FF:000002">
        <property type="entry name" value="Major vault protein-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.550:FF:000001">
        <property type="entry name" value="major vault protein-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.560:FF:000001">
        <property type="entry name" value="major vault protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.570:FF:000001">
        <property type="entry name" value="major vault protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000010">
        <property type="entry name" value="major vault protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.560">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.570">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.550">
        <property type="entry name" value="Major Vault Protein repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039059">
        <property type="entry name" value="MVP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041139">
        <property type="entry name" value="MVP_rep_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043023">
        <property type="entry name" value="MVP_rep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021870">
        <property type="entry name" value="MVP_shoulder"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041134">
        <property type="entry name" value="Vault_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043179">
        <property type="entry name" value="Vault_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040989">
        <property type="entry name" value="Vault_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041136">
        <property type="entry name" value="Vault_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002499">
        <property type="entry name" value="Vault_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14165">
        <property type="entry name" value="MAJOR VAULT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14165:SF11">
        <property type="entry name" value="MAJOR VAULT PROTEIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11978">
        <property type="entry name" value="MVP_shoulder"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01505">
        <property type="entry name" value="Vault"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17794">
        <property type="entry name" value="Vault_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17795">
        <property type="entry name" value="Vault_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17796">
        <property type="entry name" value="Vault_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51224">
        <property type="entry name" value="MVP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 9" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Major vault protein repeat" evidence="13">
        <location>
            <begin position="54"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="repeat" description="MVP" evidence="9">
        <location>
            <begin position="60"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Major vault protein repeat" evidence="11">
        <location>
            <begin position="117"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="repeat" description="MVP" evidence="9">
        <location>
            <begin position="121"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Major vault protein repeat" evidence="11">
        <location>
            <begin position="175"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="repeat" description="MVP" evidence="9">
        <location>
            <begin position="178"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Major vault protein repeat" evidence="11">
        <location>
            <begin position="228"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="repeat" description="MVP" evidence="9">
        <location>
            <begin position="231"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Major vault protein repeat" evidence="13">
        <location>
            <begin position="282"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="repeat" description="MVP" evidence="9">
        <location>
            <begin position="287"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Major vault protein repeat" evidence="11">
        <location>
            <begin position="333"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="repeat" description="MVP" evidence="9">
        <location>
            <begin position="337"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Major vault protein repeat" evidence="15">
        <location>
            <begin position="389"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="Major vault protein repeat" evidence="14">
        <location>
            <begin position="458"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="Major vault protein shoulder" evidence="12">
        <location>
            <begin position="520"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="686"/>
            <end position="763"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF11978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF17796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2069013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="849" mass="94777" checksum="E67C75F27E8AA965" modified="2022-05-25" version="1">MSAQANPSTVIRIKPHQYIHVLDNNTNVTRVEVGPQTFTRQDHERVLNTTPSSMILIPPRNYCIIDSPVIRDKSGALVLDKFNQPKLGHGDQEIRFSQDPFPLYPGEVLLSQPVPLQVVQTNTALRLKCLRDFVEVGADKKEIQHTAGDEWIFEGPGTYYPRVEVQVLETIKAIIIKENQALKLKARLGFQDRKGVVRQPGEEWLVRESGAYLPGVNEEVVGFIQALVLTDRIAYQLHALRSFKDIFGKQRKAGEEWIITQSDCDTYIPDVNEKVVRDIKAITLTNRQYCIIENPVDQATGKNRLGHKILLQGDKTFFLLPGETLVKGVQTVHVLSEDEALLLSANQQFVEKSGKQEIVHKPGDLWMIYGPCDYIPPIQVDVVEKRKRIPLDVNEGIYVRDIKTGKVRSVVGSSYMLLSNEELWEKPLPAIVQDLLKKSASRDQNEEVNEHERDPTRVVTYRVPHNAAVQIFDYRKKEARVIFGPELVMLQPDEEFTVLSLSGQIPKRPNHIRSLALFLGPDFMTDLITVETADHARLSLKLSYNWHFNIEPKNPSKLFANSDFTGDLCKATSSLVRAAVASSSFDHFHKHSAEIIQSAVFGKSEDGKPNNHLAFETNGLVITNIDVQSVEPVDQRTLDSLQKSVQLAIEITTKSQEATARQEAERLDQMARGELERQKIVDEARAEESRAKLVQLQAQSAAVESTGQAVAEARARAEAALIVAEAEIKQAKLSAKASEIEALNELEATKNKHVVEIEHLSALNKLEVSKAREVANIETSKFNNVVQSLGKETIQSIAQAGPEMQAKLLQGLGLQSFMITDGSSPINLFNTANGLIGGVPASQIQEEFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVT5</accession>
    <name>A0A8J4UVT5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SUI1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009663</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DENR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000770">
        <property type="protein sequence ID" value="KAF2069015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVT5"/>
    <dbReference type="OrthoDB" id="277199at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11607">
        <property type="entry name" value="DENR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050318">
        <property type="entry name" value="DENR/SUI1_TIF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046447">
        <property type="entry name" value="DENR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048517">
        <property type="entry name" value="DENR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789:SF0">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21023">
        <property type="entry name" value="DENR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUI1" evidence="2">
        <location>
            <begin position="99"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="191" mass="20833" checksum="89AAD8ADE3003170" modified="2022-05-25" version="1">MEPIIVEYCQVCGFPREYCEYSASAAACLKANGPYVSADAAATTTDNNTTTTTTTTTSTGVEDKLGELKVIDDSKEEGHTDSAAPVVEKKKKKEDKSIITIEVVARNKKKNVTSISGLHLFGIKLADAKKVMANKFSCGCSVVEKPGLGEEINIQGDFCEELVDLIEDKYPQIPLTSIYFIEDKKKVPARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UW18</accession>
    <name>A0A8J4UW18_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009425</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000705">
        <property type="protein sequence ID" value="KAF2069255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UW18"/>
    <dbReference type="OrthoDB" id="21188at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="120"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="252"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="185"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="127"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="147"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="267"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="305"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="319" mass="36902" checksum="55CD7E932BE64F60" modified="2022-05-25" version="1">MNGKQLEYINSGQNNPFALKERKSKKIKTINERSVQLDESSEDEALDHHYDKVINSNQNSINFEGFQDLLDLIESQPYESINDHQEVEVEVQEEEDEYNLFYNMVQNAKEKNVNVIQESFDDHDHDSEYDEDGEEEDDEEEEHDYYDGSSDQEYSQESSDINDTDDKVESNLDLFFHKAFEELERTEFELQLDLFKDEIESLRGKIEIQEDEIVKLNRILNARNKFLNSIPKDLLLNWIVTNKVVLTTNEGSVTYDHPPSPSPPSPTTSALKPTTTTTTTTSGSTKTTIKNKNTKNNNNIVPSKKIGRKKSVVHRKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWL0</accession>
    <name>A0A8J4UWL0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000591">
        <property type="protein sequence ID" value="KAF2069810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWL0"/>
    <dbReference type="OrthoDB" id="21249at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035255308" evidence="1">
        <location>
            <begin position="23"/>
            <end position="1003"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1003" mass="102598" checksum="C1602DB5D04575A9" modified="2022-05-25" version="1">MNLKFIYTIVLILFISNHAVHSAQVLLNADGCTTIACGFKALLGQAQLPQSIDDVIVYTSNAALHPIVLLQDSLNIKSLNISNGVLKIATGIAVSVTDTLFVNALATLDLESDKLLTVNSGITINGNLVLNGNGIQCNGPAVVNGNLTILSGSFEVSKLTIAATATIPNIIGGTIKVNADSSINVALNILGTATLNINSGKFTCNKGLVLAATSGLTIGNGASLSLLGTVASTFNNAITLGSNALLEVGSVVNSLKVITTVPNSAINIIANGVLNISGKNYIVAPISLSTITSKLVAKQCDCTFNQLNTLANSIISIQNASTFRTLSDITLNSIVNVDTDSIISIELGTLTLVGPVKSVAGSVINVVKGTCEIPSKVVQVINSNVNVAKDAILKVAGTVSLIGNVNCPDSSIVQLVNGILGIGGSQSVIDTVIDLSGTSQLKIIKNSICTIKKINHIDATALISVDTGSILTINIGSVFLSPITLTGSASLNVNGDASIKGIYVVPIDNTPLPSLKLFNCNSKFTGEIQRLLMSLTNTSLNIDVNVKVGALNLTADANSPIVANAGSNVDISGTLSKIQKQIQCIGCLLHIGSGTTQFLDGIITSVDSTFTTLNSTLQIGGKSILNSLTTFDGLGSVGINGDVQINGGVKCSTLSPIDINAKSTVFIGKESVIGANVNLNADATLNILPSSNCAFNGGLNTGVNAILYVNKTGNCLIAGSSEVKGAVYLNGATIVSAGTCNFYNGIKQANANATQAINNLLVNSGTCTLKDESEFSGEVAIKAAANLVLGAPVLCSKGIQNSGNLIINAAIESEDVISQTVADAVCTLNQGSQIKTKAINFVAGKLSGVGKIIASAPCVVGGIIDGAIDITGDLNLLSTAILQVDVKGLANFNKLSVSGKASLTGLIDVSLDASVAANLKVGDTMTIMECGTCEGAPKLSESTGSKCFTLSSSNTTQNLVYQSPPPVEKNAESSSHDTSSASMTSFSSFTLLISSLLLFVIVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXV2</accession>
    <name>A0A8J4UXV2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ethanolamine kinase</fullName>
            <ecNumber evidence="3">2.7.1.82</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_007134</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the choline/ethanolamine kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.82" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000364">
        <property type="protein sequence ID" value="KAF2071535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXV2"/>
    <dbReference type="OrthoDB" id="10267235at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004305">
        <property type="term" value="F:ethanolamine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05157">
        <property type="entry name" value="ETNK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603">
        <property type="entry name" value="CHOLINE/ETHANOALAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603:SF66">
        <property type="entry name" value="ETHANOLAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01633">
        <property type="entry name" value="Choline_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2071535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="356" mass="41669" checksum="F3C26BA70E487232" modified="2022-05-25" version="1">MIRSKSSYEVFHYTVLKENTEQGLREIARHFVPEYRNSSDQDLSIVRLNGGITNILYLVEDKSIEPKSKYLPVVIRLYGYKSEEIIDRKNELVVQTEADLNGLGAKFYGLFDNGCIYGFVPGNPLEHEDLSKEKYQTNIAREIAEWHSIEMPTRKTPSLWNTIKKWAALAPPIGQYPDSNKNDYYTKLNVPLMIEEYKALEQTLAKLNSPIVFCHNDLLSRNIIVDSTQDKASFIDFEYANYNFRGYEIGNHFNEYAGFGPDYSLYPTQEQQRYFLSEYLKGIHNLHDVEPSEQEITKIYIEANQFSLASHLYWGFWSIVQAMNSEINFDYLEYGKARFDRYWETKEQFLSLNNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UY91</accession>
    <name>A0A8J4UY91_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000288">
        <property type="protein sequence ID" value="KAF2072345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UY91"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035214854" evidence="1">
        <location>
            <begin position="21"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="147" mass="16472" checksum="7E87E3A911ED0EDC" modified="2022-05-25" version="1">MNKLLLQLLVAVLIVGLVSGEVERVSKKKSFPGRGVLQIRREGTNNGRFVKISLWDFKTLINNKQLSQQDIDAIKAVYDKPDFISAPNKSRFAPETVPNGHFIYIVVIPNSPNYKTFAWTSGYPGSPAWLHLFENLLDKYGIPGNGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYC4</accession>
    <name>A0A8J4UYC4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_006177</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000273">
        <property type="protein sequence ID" value="KAF2072510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYC4"/>
    <dbReference type="OrthoDB" id="24136at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052014">
        <property type="entry name" value="Dictyostelium_Tiger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31341">
        <property type="entry name" value="IPT/TIG DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035209051" description="C-type lectin domain-containing protein" evidence="5">
        <location>
            <begin position="19"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1107"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="6">
        <location>
            <begin position="28"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1040"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1056"/>
            <end position="1095"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2072510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1157" mass="127935" checksum="FD5D2046563107FE" modified="2022-05-25" version="1">MKAFCIFAVVVLLKLVQSQQLTLVLNQENRHYYAYSNTKAIYQDAINVCQTYTPPDGFTGYLVTVTSKDEHDWIGSHLGAISSVWIAGSDLGDVGNWTYNVGPEKGQIMYDLYSERTYTFTNFPYTEPNFQVGENYLVSNPNSKDGWNNVVASTQSNYICEFSPIDEPFIPTVHSEGDMVTVNVSGYDIATLEIKFTSVSQPNFNCQNIKKVGENAITCQIPSGTGKYSYTLSDKVKSSKSVPWQYHPPHISVIVPSLGSGSTVTLVGDNFGNDASKIQITLDITGTNTECNNINIITPFKKMSCSLKQLLPKILPFQVVVNSISSRYNKTSIYHDGSKSIFYISFYTATKYSVMLDYSKLTKVDGNTGYLSSFDNEAQFKFVKQYYPLTSPWYISLGIKIETIDPLLYFYTAGPSIGGSADNFYYKYFGTNPDWMREESYVTMDFEYESIMTQTIEDSRTIYGGLIEFGGYLPTVRNYQTPYLIDTDGGTIFVQLDNFGTSLSYTSVQTTNLLSFSISYYKGGISMTISHVDLVAQKIPIKITIDHVEATPVIYYDRSHPSITNIKPSIDTAGGVITITGASLFNQESAITVKGIVCHDIQFTTPHKQFTCSIGPGTGMKSISIDVGGKVSNTFNYNYQDPTITSIPPIPTSGGIVTIQGTNFGIDKTAISINQCTNIEIITPHTTITCEFNQGQGDLSVTLTMGTYSTPPFKTRYQYPTISNIIQTIGNIDIIGDNFGWDQSKLIIKMDNYLVIGSSCTLVSNTNIKCNNLATVYGPFSISNDNGVNFGTTYNYDLKPQITSTFEGVCFNPQEKLTIKILYMISKSSTFKIKYRGQYYQVNILDSFTSEFIIPDGSGIVPLQFETDNTRLSNEVSISYCPTVIKSIDFNIRTRKYEITGTGFSSNSNKGLITSTNGLSKLNLQVDQFNSTFISIGLPMDAKSGLVMIDTSYQLSNKVWINITPEIESASNPLEQGSPITITGKYFKTVDIDGNNVGLEFSINGNRLTCEAGSNETSVVCQVPPGSGKIIINVKSLSAKNGESDSTDLTLTYIKSPNNNNSSNSESNNNNSHSGSTNNNNNNSSNGPSNINNDQNNSNYNSWKFKAPLIVVSCLLGVSLSIGVFIFLRSKAASDFFNRFRSRRSRMHRNEISFELM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYJ5</accession>
    <name>A0A8J4UYJ5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ferroxidase</fullName>
            <ecNumber evidence="3">1.16.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_005772</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>4 Fe(2+) + O2 + 4 H(+) = 4 Fe(3+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.16.3.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the frataxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.16.3.1" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000239">
        <property type="protein sequence ID" value="KAF2072915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYJ5"/>
    <dbReference type="OrthoDB" id="1897642at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034986">
        <property type="term" value="F:iron chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.920.10">
        <property type="entry name" value="Frataxin/CyaY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017789">
        <property type="entry name" value="Frataxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002908">
        <property type="entry name" value="Frataxin/CyaY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036524">
        <property type="entry name" value="Frataxin/CyaY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020895">
        <property type="entry name" value="Frataxin_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03421">
        <property type="entry name" value="FeS_CyaY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03422">
        <property type="entry name" value="mito_frataxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16821">
        <property type="entry name" value="FRATAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16821:SF2">
        <property type="entry name" value="FRATAXIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01491">
        <property type="entry name" value="Frataxin_Cyay"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00904">
        <property type="entry name" value="FRATAXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01219">
        <property type="entry name" value="Frataxin_Cyay"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55387">
        <property type="entry name" value="Frataxin/Nqo15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01344">
        <property type="entry name" value="FRATAXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50810">
        <property type="entry name" value="FRATAXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0409">Iron storage</keyword>
    <keyword evidence="6" id="KW-0410">Iron transport</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2072915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="180" mass="20961" checksum="98CF671D50E82602" modified="2022-05-25" version="1">MIRLRNLFKYNSGSRNVLRVYNSSHRYPLALNNNNDQSISVSLNNNSNNNNNNNNNYKIRFYSTQNEILDENAFHDIVDVELELFVEKFEEIGQDLDLDDFDVESAAGVLTLKLGSLGTYVINKQTPNRQIWWSSPISGPKRFDYDSKSKQWLDNKDANVNLRSLIKDEINQLCKYNLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZ50</accession>
    <name>A0A8J4UZ50_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_006202</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000275">
        <property type="protein sequence ID" value="KAF2072493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZ50"/>
    <dbReference type="OrthoDB" id="10249311at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000039">
        <property type="entry name" value="40S ribosomal protein S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000530">
        <property type="entry name" value="Ribosomal_eS12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11843">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00972">
        <property type="entry name" value="RIBSOMALS12E"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="5">
        <location>
            <begin position="24"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2072493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="141" mass="15477" checksum="4359DE5806AAA000" modified="2022-05-25" version="1">MSEGDVVSTPSVNPLEKVTDPMVALQKVIKEANAVQGVSRGLHEVVRALDKRVARLCVLADNCDEQNYTRLVKALCAEHKIPLIPVADNKVLGEWAGLCKIDKEATARKVVACSSIAITSFGKESEEYKFLMEYVEKNSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZG3</accession>
    <name>A0A8J4UZG3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_005700</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000234">
        <property type="protein sequence ID" value="KAF2072988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZG3"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09358">
        <property type="entry name" value="LIM_Mical_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24206">
        <property type="entry name" value="OS06G0237300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="5"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="116"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="151"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="168"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2072988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="213" mass="23495" checksum="4EAE55513B6B6571" modified="2022-05-25" version="1">MSAAKKCASCNKTAYPLESIVALDSSYHKACFKCSVCNMTLNVKNFKGLEGKLYCAVHTPKATATQVADSVAIKNALNAPKKTAEGLGNAQKGSGDKPTIGLDTVANKNALSAPKKVAEGARGVQKGTGGKPQIAVFGATPGAQSTGENIEYAQEEQQQYEEQQYEQQGEEQQYEQQYEEQQYDENQYEQQGEEQQYEQQYEQQGEEEYYEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZK2</accession>
    <name>A0A8J4UZK2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004126</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000140">
        <property type="protein sequence ID" value="KAF2074580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6161812at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008782">
        <property type="term" value="F:adenosylhomocysteine nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008930">
        <property type="term" value="F:methylthioadenosine nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019284">
        <property type="term" value="P:L-methionine salvage from S-adenosylmethionine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46832">
        <property type="entry name" value="5'-METHYLTHIOADENOSINE/S-ADENOSYLHOMOCYSTEINE NUCLEOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46832:SF1">
        <property type="entry name" value="5'-METHYLTHIOADENOSINE_S-ADENOSYLHOMOCYSTEINE NUCLEOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NB-ARC" evidence="1">
        <location>
            <begin position="429"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="2070" mass="240561" checksum="10104EE1751F4BD1" modified="2022-05-25" version="1">MNQHCVCILHSGHTEVLSTISIFEKNSAIKFSDVKQSSDFAYREGVLEINNNNESDNTVRRSIRVILSFPSNQTSGEIVLNTSHVLDTFKPSLAIIVGTCSGYKDNETPISRGDIIVGQSAFNYQTGINTNDGLRFTLNDCIQSNKSLLNALKLTDHSKSDWIKYPSIEVSTTYKREWLLRAFFEYQDHKDNLEKSVWLRNQYFDIKKTPARNTVVQKMFGNQYNKEKDELVKDGLMEQDENGIPNISDKGFKKMDILLNRYGDYPYNLFKEIKPNIHFGQFGCGSALEQQYEVVDKKRISLAFKQCREKSTGIIAIDRDTHAFYLTTQNYKEISFISVKSVLDYADGDTNQNNNYIDYCNQLSSSFALHLIRSHSFESNDFQLIRNFIPPPPIKSINRLGSDNKGYIQLISDSFKSKSKSNSVTISSRISGMGGVGKSTLAKQFALHCIDNQLYKYIFWIHSETQETLFESYKQILQHLNVEIKNQDNIVTAFASSIQQLQLKGNVLLIYDNVEDMSLIREKPQGNHINILITTRSQMCDDPSSTLIPLDLLNLNECKSLFKLWRPNITDTHIAELSILLQRLPLAISHCLAYMYQEGIEEWEYIKEFEMFNEIQVIGDDSFENPYEILIGKTISMAIGKMKNKQDIESRATTVLHIMAFLNPDQIHSKVLEIILKGKDNHINAVIKILKNFSLISTDQEANSNQNYNISIHRVVQRSIIVLLLQKLKNNNSINLIYDNLSHINNQISTYFYSNNTNINIRNSNSNSILLDNYNNLNNISIHLTNIQTIIQKMDNLPHQQNLELTNKLLLTQIIMECNHLSYLILNSTVNRQVLEALFQEESNIDKNNISELVDITFQFINQNQQLTQGIDSKIALNIFSKFMSGESIDKDKCNIILKFIFLSLGKIGHFLDKDIQFFKKTSEISILRISLINELLFRLPGQVLNFSQLQNMFTINPIASDNDYSILIDKLVHLNDKVKGLDRSDTEIIFNSFNSFKSIDPTIIHFTTNLVNESTNDNHISKIILILIKFKLTFKNDKAKIVFNYIEELFDIGSFKDFANKFLEVLSKINFDKFNCGQIEKLFNYTNELINEKFNGHHISFIISTISEQTTKYRIHSDQIKNIINYTKQLINEKMNSYDISSIISTLSEQITKYQLNNDQIETIMNYTKQLINENMNCDHISTIISTLFEKITKFKNYQIQSIINHIKQLINMNHDQLYKILSTLPDQITKLNGQILLNYCTKQLLNGYDIYLIKSIISEHVTKCQLNNDQIQTIINYTKQLINEKMDRYVISSIISTLSKNFKFNNDQIETIINYTKQLINEKMDRYDISSIISTLSDQITKYQLNSDQIQTIIYFTTQLINEKMNGDHISSIISILLKQIIKYQFNNDHIQTIINYTKQLINVYMSGNQISRFISTISKQITIYQFNNDEIQTVINYTKQFINEFMDVYEEKFECDVSKLYKKISKNQLNYETSKTAWPLPNEIVKYQITKDQLINYTICTLSDYQIRYICYPAKQSIDEIMDGYNISSIISTFSEQITKYQLNNDQIKNICKLTIQLMIEGMNGDQISIILTLSAQLYKYKLNNDQIQNIINYTKQLIDEKMNGDDISSIISSLSEQITEYQLNNDQILTIINYTKQLMNEKMNGDDISLIISTLSDQIIKYQLHNDQIQNISNLTKQLINEKMESYHISSIISTLSKQITKYQLNNDQIQNITNYIKQLINEKMNGYEISSIISTLSEQITEHQFNNDQILTIINYTKQLMNEKMNGYDISKIMSNLSEQISQDPSNFKECFKNNIILKACSHLGFRYSFSYILHIYRNNPLAFEQSYICILSLNQIIESNQFLNFLEIVIACNSEERNYIMAKLTSEYRDKINAQTNLAQDKILSMKEKDNCCLKNRHNSLDENDTDARVLFTKLNDVKEHIQDYDFKILSRQFSFHPNLFKESIIKSFIKIKNSLGLVLKQHGFQYLIFQSLYPYDDDQLELVCDIIEKTREHKQEYFCCDLHIANNIQRLYYYNYKFTIGNNNFLQVFYSQLKDIDNNSNLECMKTVLLNTLNPKIRFNANI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZL4</accession>
    <name>A0A8J4UZL4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005440</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAP18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000217">
        <property type="protein sequence ID" value="KAF2073243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZL4"/>
    <dbReference type="OrthoDB" id="440566at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.550">
        <property type="entry name" value="ASAP complex, SAP18 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010516">
        <property type="entry name" value="SAP18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042534">
        <property type="entry name" value="SAP18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13082:SF0">
        <property type="entry name" value="HISTONE DEACETYLASE COMPLEX SUBUNIT SAP18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13082">
        <property type="entry name" value="SAP18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06487">
        <property type="entry name" value="SAP18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="127"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="178" mass="19932" checksum="060276D4AFBFE023" modified="2022-05-25" version="1">MISYNSKPCPLLLRVFMKEGSFHDTSSFISNSEVPEGEANLHVWKHSTLRDIVTLLKENNETSRKKDIVFEIRFIGPNEKGLYNHRYIGSINSSATKKTDLDSKTLQDLNFNYEFISICLKNTALPSPSTTTTSLQDTPMDTTTTASKEEEDKETSSTTTTTTTVENKESTADTTADM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V016</accession>
    <name>A0A8J4V016_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">MHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_003667</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <location evidence="2">Coated pit</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000118">
        <property type="protein sequence ID" value="KAF2075015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V016"/>
    <dbReference type="OrthoDB" id="10259133at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030131">
        <property type="term" value="C:clathrin adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09251">
        <property type="entry name" value="AP-2_Mu2_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14836">
        <property type="entry name" value="AP2_Mu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000002">
        <property type="entry name" value="AP-2 complex subunit mu, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043532">
        <property type="entry name" value="AP2_Mu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018240">
        <property type="entry name" value="Clathrin_mu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043512">
        <property type="entry name" value="Mu2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005992">
        <property type="entry name" value="Clathrin_mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00314">
        <property type="entry name" value="CLATHRINADPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00990">
        <property type="entry name" value="CLAT_ADAPTOR_M_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00991">
        <property type="entry name" value="CLAT_ADAPTOR_M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0168">Coated pit</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="10">
        <location>
            <begin position="172"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2075015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="435" mass="49525" checksum="08E9C800BB958972" modified="2022-05-25" version="1">MISALFLMNGKGEVLISRIYRDDISRGVANAFRLEVIGSKETRSPVKLIGSTSFMYIKVGGIYIVGVSRQNVNACMVFEVLHQLVDIFKSYFDTLDEDAIKNNFVLVYELLDEILDFGYPQNCSTDVLKLYITQGQGKLKSLDKLKQDKISKITIQATGTTPWRTPDIKHKKNELYIDVIESVNLLMSAEGNILRADVSGQVMMKCYLSGMPECKFGMNDKIIMDKEKGSVPARSQRRTNGIEIDDITFHQCVRLGKFDTDRTISFIPPDGEFELMRYRTTEHINLPFKVIPIVREMGRTRLECSVTVKSNFSSKMFGSNVKIIIPTPKNTAVCKIVVAAGKAKYMPEQDGIIWRIKRFPGDTEFTLRAEVELMASVNLDKKTWSRPPISMEFQVTMFTASGFHVRFLKVVEKSNYTPIKWVRYLTKAGTYQNRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V072</accession>
    <name>A0A8J4V072_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phospholipase B-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009841</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000826">
        <property type="protein sequence ID" value="KAF2068837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V072"/>
    <dbReference type="OrthoDB" id="301415at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035159621" description="Phospholipase B-like" evidence="2">
        <location>
            <begin position="22"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="526"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="556" mass="62562" checksum="1D4E912D03C20A47" modified="2022-05-25" version="1">MKLKVLGILLLLLNVNIYVHSFDTGHHGDITANAMQIRGYGKSARDVSVLLNWFPDFFAYTPIQHIKEAQLLHFDNIYTLQNATDYFGQLIYNSKSGFEQIAKEGNTLQYLGLLGVTLHTIQDFYAHTNWVEIHSPYDCDCVRSETFMEGIHAANGNLTQLYGDVRSLASYSWGEDCSADERNCFPGTNVHGDYCGGINKDSYVRKSWQQAYATAFAASVEWIYNVELWVNAVNKEIVTNAKAYTPATETDQNDLSSNLYDSVVVSYGAKTTKDDGHYKGPGSGSLKRFGYYSERFLASNSIYKKLFTQDKIYNYLVTPDIYNASTVKANTKSPDLLPFVEVYHPLTYFEKNLYSSAILRTTYVKTDDKFNSPSPFAIVTMGPLEFIDAVQIDKTEFRPYWTDFFIFPFSQNESVPIIYKLLNDKLLDGNEQMNLDDNDGVISMTMQPKTAILSGEATGLHNTSSSELTYVLGGTTVKILVMTKQIGSCSLSSLPDPICGEWAWPQQPLNQYCDVSELVTSSSSNLILMFTSSNIMSILLLILSFTFMIHSAFRNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0P2</accession>
    <name>A0A8J4V0P2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_002649</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000075">
        <property type="protein sequence ID" value="KAF2076035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0P2"/>
    <dbReference type="OrthoDB" id="439639at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12565">
        <property type="entry name" value="RRM1_MRD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12317">
        <property type="entry name" value="RRM4_RBM19_RRM3_MRD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12320">
        <property type="entry name" value="RRM6_RBM19_RRM5_MRD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000442">
        <property type="entry name" value="Multiple RNA-binding domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF58">
        <property type="entry name" value="RNA-BINDING PROTEIN 19-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="4"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="349"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="536"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="661"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="778"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="162"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="251"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="430"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="614"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="743"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="122"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="207"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="254"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="265"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="307"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="327"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="447"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="646"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2076035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="878" mass="99971" checksum="5E175EAEF8CDD143" modified="2022-05-25" version="1">MSNTRICVKQIPKHLTEKRFKDHFEKFGIVTDAKILKTKDGKSRLFGFIGFSSEESAQKALSLNNTYIDTSKIIVQLATSASETNVETNRPWSKYSQGSSSFKRIEDDKVKKESRKKEKELLKLKKQQDKQKKQNPLLDQDLENDEEYQKFKKLMTPRVNKKLWENDDEIGVGGKKKDNDSDDDDSGSDEEGGSKKKRKVDEKLAFEGDDSDDELYEEMPAVQVKTAEEEEQEALELLKKKDKLVNDSAVSDLDWLKSKVKDVQDTDMIDNQDEEDEDEDEEDEEEDEEEEKPTKKNKSSSKQDKQEEQEEEQEEEEEEEKEEKEEEQDKKKEIVHKEKEEIEDVGDSGRIFIRNLPYTAKEEDLEQLFSKYGKISEIHVPIDVASKRTKGIAFILYLIPENAVQAMNEMDGKAFQGRIIHILPAHSAPNKIPLQQEGDQEGSSSFKSEKEKKLKSESGSNHNWNTLFMRSDAIVSSLAERYKMTQGELLDPNAVDIAVRMTLMETHIINETKKFLQEEGVIIENIGDKVSQRSNTVILVKNIPFRTEEAELETLFGKFGSLSRVVLAPARTIALVEYVHPTEAKIGFKNLAYSKFHFVPLYLEWAPLSIFKSGPKPKQTKEEKDTKSSTSPSTSSTKEDQEKKSNTSNTTTPPSSSANSHFVYIKNLSWKTSNENLVTRFKNLKDFVNATIATKADPKNPGKRLPVGYGFAEFSSKQGAYECIKKLNGSTIDGHEINLKLSDKNEVNKTADSSAGNKRTREDSKSTNGTATKSPVSTKIIIKNLPFESTAKEIRKLFSTYGEIQSVRLPKKPNGGHRGFAFVEFLTEQESKNAMEALTNSHFYGRHLVLQYAEQDKNIDELREKANTDYDKIKSSKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0R4</accession>
    <name>A0A8J4V0R4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002587</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000072">
        <property type="protein sequence ID" value="KAF2076125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0R4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31318">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035304830" evidence="2">
        <location>
            <begin position="21"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="461"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="463"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="551" mass="57550" checksum="08E8F555C29ABFF2" modified="2022-05-25" version="1">MRDYCLAFLLCVLSISVVLSTPTTIYVSPDAVNSKVQCGTSIQSPCGSIVDAIASFDSYSFPGSGNPKLYPPLVIALLPGTYFSSATSKNVSDTVVFYQHDITIQPATPNTDVVISGKYFTNDLFNIGLSKPAEWGAKLTVTGITFDSWSSQILAASSIYDLSASFSNCTIQNSAGGGGNNPSIFLIYSYGSSKITFLNFNKCTMDSNSAKASSSLIYTSGVTVSITNSNFNNNNFTTMITSSSIVTMSNSNVTNSGRVAYAINGNMTITGCNFKNNQDSSYIFYSVKSGVSISTSNFISNSIGSSQNGILTISGAEGYYFTVSGSTFSNNNGTPIYSTGNNLAVKGSTFQNNQGYYGGGVYQSDTIADIASSIFIVTSSGNSLSEMIYLSKVPSLSIESTNFKITSDATGVSSKFSMIECQGSAIYLSDVTMDLAQYRSIDCTSCSVTIRSGNQYQCEVAPTTSSTSSSDESTSSTSSSTTSSSSGTTGNSGNGSTTSGASSGNGGTITTTSSTSTSTSSTTTHTTGDANSHKIEMWVYIVAALFALLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1F8</accession>
    <name>A0A8J4V1F8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Threonyl/alanyl tRNA synthetase SAD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008237</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000491">
        <property type="protein sequence ID" value="KAF2070442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1F8"/>
    <dbReference type="OrthoDB" id="16238at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="246" mass="27134" checksum="132DA5D77AB6F776" modified="2022-05-25" version="1">MSKPRDSVTDSACHVLKGAIVKILNTPLTVSVECNNKCKGRINVEYDSADKPSDDVIKSIQDEANRIIKENLEFKVFSCDRKEAEEKYKSQLVNNTFIYDKFPVPESVTTLTLVELQDWNINCCPAAHLKTTGEIGSIKIVGINHRPAKKELEFRFDIGPALTEQTTPAAAKGKKAAAPAAAPVIETNSLTLKDTNVQYLTQKIVDLVLSTKGTKEMIQESTIPQIEQLLTTLKNTSYANGFTCSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1L8</accession>
    <name>A0A8J4V1L8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010440</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001114">
        <property type="protein sequence ID" value="KAF2068234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1L8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053370">
        <property type="entry name" value="QS_Complex_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35035">
        <property type="entry name" value="DISCOIDIN-INDUCING COMPLEX SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35035:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="709" mass="79173" checksum="4546BFB6EF3C3ED3" modified="2022-05-25" version="1" fragment="single">MKIRFEFLLIYFFIFGVFCQIQTITWNAPSGLFSDASKWQPNVVPNSDQIAMIDNGGTVEATSPIAVFSLAVDLGSTLTTFDSTHAQNMILVDRNAILNLFRDDIQFYVMKNYAQVNLKGHSGYTQLDNYHELNIKGKPNLVGPLIQNFYNMTVYFSSPTFYGEVNNFGSMEILGTKENTTTFDDYYAGSTSDLFIINTNIGFNKGFILLDSFSLIESSYIDAKNITFKNNSIVYIQNSKFEAFGFLLSNYTSLNIFTSNCTFGGNLGDESMFLVINTITNIQESNFTFQDKKISVLGKVPGKLFSNQLVWSKGSKVLFNNTGFVLSDNPLLAVYGESEFNFVGGNFLAQNETVILCDNSTITLFSPRVTNNAFYIRHNSMVLLLNSSKILLNGLMVFTENGNLNVENSVLWLMGEVQMMDDSVLSFKNSREISLIYGNLRLLDRAFINSDSSFIAIWGKIHLKGETDIRLKKSTLIVPGEILMEKDSFFYANHSNIYVNGTVNSYGTLNALDIFFNVEGKVNFYNNFAIFKDSDIRIKGDLNMYSNQTNLFDSIFTLINTTTVVSNNLKISGSLFVATNSTIEIHGRFIYESGTIFLFNSGFRNRVGGLLDTTSNIEIDSNSTFVNQGTILLSSNIIPSNSTQSVDMLMVENSGVINVVRDGSNITIPLLNTGEFNLGNKTIYVEQYSQNLGMLLLKGGFINSDKDIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V362</accession>
    <name>A0A8J4V362_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA polymerase II core subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_009246</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the archaeal Rpo5/eukaryotic RPB5 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000663">
        <property type="protein sequence ID" value="KAF2069434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V362"/>
    <dbReference type="OrthoDB" id="248779at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1340.10:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.940.20:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1340.10">
        <property type="entry name" value="RNA polymerase, Rpb5, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.20">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00025">
        <property type="entry name" value="RNApol_Rpo5_RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014381">
        <property type="entry name" value="Arch_Rpo5/euc_Rpb5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005571">
        <property type="entry name" value="RNA_pol_Rpb5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036710">
        <property type="entry name" value="RNA_pol_Rpb5_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000783">
        <property type="entry name" value="RNA_pol_subH/Rpb5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020608">
        <property type="entry name" value="RNA_pol_subH/Rpb5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035913">
        <property type="entry name" value="RPB5-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007129">
        <property type="entry name" value="PRK09570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01191">
        <property type="entry name" value="RNA_pol_Rpb5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03871">
        <property type="entry name" value="RNA_pol_Rpb5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000747">
        <property type="entry name" value="RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53036">
        <property type="entry name" value="Eukaryotic RPB5 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55287">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01110">
        <property type="entry name" value="RNA_POL_H_23KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase Rpb5 N-terminal" evidence="6">
        <location>
            <begin position="3"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase subunit H/Rpb5 C-terminal" evidence="5">
        <location>
            <begin position="128"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2069434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="201" mass="23405" checksum="6F799099752F2B5E" modified="2022-05-25" version="1">MEDLNRAYQVRKTVLQMLSDRGYLVANSELEQTKEDFKKVHAAGLKDNMRYLKKDDPSKQIYVFFPTEPKVGVQPIKKYVSDMRDHEVDKGIIVVKQNITPFAKQALANFSQAKNITLEQFNEAELLVNITHHQLVPKHVLLLKEEKFELLSRYKMKESQLPRIQINDPVARYYGLQRGDVVKIVRPSETAGRYITYRLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3F8</accession>
    <name>A0A8J4V3F8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008947</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000604">
        <property type="protein sequence ID" value="KAF2069734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3F8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="250"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="295"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="383"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="465" mass="51627" checksum="D10726CF348786EF" modified="2022-05-25" version="1">MVVNNNNSKENDKSEWVGEIVILGVDNIAPIGKIPIHKPIYFGSDQSKCTVLFNDSKEQQQQKEQEQEQKEQIEKSHTQDQEKEVMQIEEKENVKELDKGQEKEQMHVQEKEKQQQKQDSVYLRLKVYTIKKKFDKAYITMLTDTGVYIDSLPNPFLNREIQLKPKEIFRVCNRSFYFSITKDNTIPETFTDSTISFNSVATPQTPIQTRSKSLLNSNNNTPTTPTTPTTPTTPTTPTTPSIIASDKKNTGSTSLGSSSSDLVNTDINLTNIFEKEITSSNKQQETVGVTQQDNQQSPKAKSPKKQTSNESNSSTPSRLSPLPSKPIKQLLSPKPIASNGKKFTYKRKSIDGGSSTNQGVDSNQAIVVEEEEEQKSTPKKIKSSTSTSTSTTPTKSPTSSSLPLKSPPSAKKPNTPLKSNPPTPTTITPSPPKRKTPTKSKSPLPANFSPLPRSSRRTFHPVSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3P1</accession>
    <name>A0A8J4V3P1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nuclear pore complex protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_005913</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as a component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
            <location evidence="7">Nuclear pore complex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the nucleoporin Nup84/Nup107 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000249">
        <property type="protein sequence ID" value="KAF2072767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3P1"/>
    <dbReference type="OrthoDB" id="3098at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3450.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007252">
        <property type="entry name" value="Nup84/Nup107"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13003:SF2">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13003">
        <property type="entry name" value="NUP107-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04121">
        <property type="entry name" value="Nup84_Nup100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="5 7" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0811">Translocation</keyword>
    <keyword evidence="1 7" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2072767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="906" mass="105075" checksum="A50838838CBA5790" modified="2022-05-25" version="1">MSNRLFQWNDEDDNLSSSFNNVNNNNNNSSSIFPKSTSLFNNNNNSNYNIDFLPNDQDDDLMMMNDNKPLGINRVNSSRQFDDMMDSDIFNNTNDNQMQYNIDNSQQQEQQPQSQPQPQQVEFDYENIFNEKNKTDDFIYHYTLESVDLQTGNESKENIPIHLALNFLKLSQSKINSLKNELEQSYYLPNKKQIENNMHLYEFEKDTWDILSKLYIYRDIEKEKDRQESIVLQMQPSQTEVFNYLMKKNFEIRENSIIIQWLEEMASHIELNLDPVYWDHTLQDLKNKNKKTTAGKTTGNLVTELDADASSRQNVSIDKEDEKNQAKFLSTLWAFLRAGNKEGAIEFCVTVGQYWRAQTLLGFNYYDNETNIGNPYFNLWKSNCLDLSNKSQDLYERAVYGVMSGNLNSILQVSKNWYDYLWGYLKVLFDERIYQELKPYRSALSVEEDIEHLAPSSNLSQIHTPRDIINLLKTASPQDIKNQSKNPYHIIQQMIINDDYRALFDTCAQLISKTHQPIEFTRFAVMLILFFRKRQCYQDDIENGLDTGADDENSSENVIIHEYVKHLIGTQQHELVALYTSLFNSEKLRITVYSKFLETITDQATRQQCLGLAEQFNLNTQEIAQSVVKNITGSVSASIAESSSNNNNNAISSSATTLEDSTKIDSIKWLCFDKQLLIKAILSSNLLIREFIKSNKYNASSDLLKSLPKDIVAIAKQSSPLDEQDTNNIIKEFKDWENYLSANNRMNTWLQNYANQPKENLLSFKLSGKETYAQKLDIERRKKDYEEAFGQWNQNNIIHGQEAIAAINTVLGSGWLCPMENPVGEFDDDDENGIEINEIRKRCIPSLFKSLHTVLIGTDQMAKSSRLCNKIADERFKWFNVFTKSELQEILFLVRESSIKMLSSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6V3</accession>
    <name>A0A8J4V6V3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Myotrophin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_005421</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000216">
        <property type="protein sequence ID" value="KAF2073259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6V3"/>
    <dbReference type="OrthoDB" id="5946465at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0085020">
        <property type="term" value="P:protein K6-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF8">
        <property type="entry name" value="BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="38"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="71"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="97"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2073259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="13198" checksum="E3564466D1D98AA8" modified="2022-05-25" version="1">MSSEKANELNWAVKNGDLQGVHTLIESESSLVSYQDANGRSPCHWAADFGQAKVLEYLVSKGAKFDEKDNYGITPLLASVYEGHVDVVAFLVKKGASKSVKGPDGSTPFEAAEKPEIKNLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7Q9</accession>
    <name>A0A8J4V7Q9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Telomere-associated protein Rif1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_001624</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000040">
        <property type="protein sequence ID" value="KAF2077057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7Q9"/>
    <dbReference type="OrthoDB" id="5399929at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140445">
        <property type="term" value="C:chromosome, telomeric repeat region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022031">
        <property type="entry name" value="Rif1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22928">
        <property type="entry name" value="TELOMERE-ASSOCIATED PROTEIN RIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22928:SF3">
        <property type="entry name" value="TELOMERE-ASSOCIATED PROTEIN RIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12231">
        <property type="entry name" value="Rif1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="Telomere-associated protein Rif1 N-terminal" evidence="7">
        <location>
            <begin position="63"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2077057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1166" mass="133276" checksum="BBE4930C097C21A3" modified="2022-05-25" version="1">MSESYTNLQKILRVLESNLSAQSIINTKTIVSVEESFKSLLTTLKIAEKDEDILNSLSNSVLIQKYLSVFSHHISSSNNLQQQQSASSKQQQQQNQQLLSFIDKFAIYSLRSLGYFIFLQPIVQNLQESTIKEISSILENTILVTKNKNICNYALWCFSSQNLPNGPISNKLPSTYTQLLATTPFESKTVEAEILTSFYSLLIQNPSPHLLLSTSWLPSIFIKMIDKTNAPEISEKLTLTVDYLKKLYNESPEMFPDDFSNGLVDKLEMFLEGLMNLFESNLEERAITYWGYFICFIKKHIFPKNLMNKLLKLPEKCFLLEKKEARLLAFKSWRSFIDALDTHTHTTPKRLKLIVSPLAIGFESAEVEIKKESFKTWLYLLNALNAYTKSVVDFNVYFSPLFETCMSCPELSTYALHLLAAYVSPSKDVRKESKLVLKDEFDCIVGTLPLQNIPITSKWVLDNFNTFTMAIQSLGQELQKQKLEIWTGFLKRVFNIDQIKESPQFIQISQNIFLFIQMLLATLDINGKSEANLNYYKEIFSSTLKSFPVSNLISSTCKVELNTKSFIYIDFLILNILEFGFKLNSEQYKELVLPLFEEMLVQIAFSNAHDFSQKISFVLDIINDNIPSPTSSHPDIVDRNELEKIFVYLLDIWLSLLNNVKTNIEKSHQLLSLPTSSINHLIDAFEHLLIWPMKMISSHSKDCKRMTIIIENSKLWRNTFGSIVKIFSTKTNLTPFIIRMIGQVGNFRYTNKEYLDFILLVLSIFINELLNGMSSSSSSSTATTTSKKRSSGEMSGYYSTILESFISIDKVLIDCHFSKTIVEKDSTFLLLSNQLSVFNGLSAIVKKANSFEFTLGALKNLKNSLHLYINSPKPDNQLLEKIQDHQDKYQTTLVKRSIQQFWETCLETIQNTKSKEKGSSSSPFGNNDLLVEMEETLIDGFKCKTAEIKECTLQFWNSTFGQSTSLVYPFKLGTLLVKLKEKGSQVFMDTHAISNSNSNHRMSTYGESQATLGFDNDYQSIDIPVSQFQKLNNQNGKLRNQVIKEPTIATNTAPTKRDSINNHNNHNTLKPAIKKVKMDEYVNVLPDTNPDASTQSLTEHQMEVLGREIRTQSFGLSEVEDIEGQITTIYNKLLENQSTRSLLSIQQISLKISERVNRILKERLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V820</accession>
    <name>A0A8J4V820_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_004146</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000141">
        <property type="protein sequence ID" value="KAF2074564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V820"/>
    <dbReference type="OrthoDB" id="21456at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035156657" description="EGF-like domain-containing protein" evidence="4">
        <location>
            <begin position="20"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="608"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="549"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="706"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="706"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="730"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="553"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2074564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="740" mass="82910" checksum="C3ED9645A49D18B1" modified="2022-05-25" version="1">MNNIQFLILTICLVYSSLSITTSNALDSWVMPNHDSFHSSFSNSDSIVNDIHWLNVTYNDEASTSDTYTYNIKLQYNPLIFLNQSFIYLKSLGCNKDICNLVIVKNYYQGDFVWSNQIKFNSSLLEEKETLDIELISSSGSQVENEIVLVYLTNINQFYSFDSQYGSFLWTSFSNSSNSSENSNSSDSNELTDIYNHCTSPLQTNNGSSATINTQYYMLCNNYLLNDTLEFILINFSTGNFKNFKINNNNDIDHLSILINNNNAFNSILLLPMPNDLNNIIFYQYLNNNTDSNSNSDSTSSDSNNNSNNNNNIKEIIYIFSLKDFSIKSSWIPTLKNSAIGRLINSNSIIVFNSTISMLYCSNNKTISIDRFDIASGSSIGTKVLLDGLRDCNPNTIVNAKLGYNSITGDIIFMIELKNRVDKIISYLGTLISSQSLSYSLQWIVLGDKQSRIYDFSLSLPNSVYLVRDNGLSLYSYTNTLIQFQSFPSNFVIVNDSLPTISNGQAYVLISNDNVLLKAFDSICSEQDYCSGNFTCSSLGCVCKTNYYPNDLCQVYCDPVATCSNQGTCDENGLCVCDPTRGYIGDDCSVCRKGWYGADCNLHFNYKMVIIGLSVTALVTSSICIFACCTKNIKSKFNYTNDFTKNTGAPPTDYDGDYQVEILNETPTPPPTPLLKRLKLLFKSNSSTSTSNTSIFGSTKNSTSINYQPLKETNNHNNNSNYQILNDDHISDDDNDNDNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V9B0</accession>
    <name>A0A8J4V9B0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HTTM-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002762</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000080">
        <property type="protein sequence ID" value="KAF2075914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V9B0"/>
    <dbReference type="OrthoDB" id="409811at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011020">
        <property type="entry name" value="HTTM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052964">
        <property type="entry name" value="Sporulation_signal_mat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39535:SF2">
        <property type="entry name" value="HTTM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39535">
        <property type="entry name" value="SPORULATION-DELAYING PROTEIN SDPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00752">
        <property type="entry name" value="HTTM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="190"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="276"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="299"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="408"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="500"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="HTTM-like" evidence="6">
        <location>
            <begin position="35"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="734" mass="86320" checksum="A812813B142D6A8A" modified="2022-05-25" version="1">MTTTVCNRRSTAGSNNNSLVRNFIQKAELFNLSKCFYLDKRSITLYRICLALFLLADLFFRSLYILKHYGSWGVLPSSFAVRPDRYSFHLISGNGMFLSGLFIFHAIVYLCLLVGYKTRVSTILSFIMLCSLQNRNHYLLDGSDDYTRVMLFWSIFLPMGSYYSLDSIIKSLKVKKFKLEQQKESSKQENHYLSIGSIGFIFQFSVIYIFTALLKYGDAWHKDYTSVYYAINLIEFKWGISDLLLQSSFLLQFLAKFTLLFEYLGPLMIMSPFFNSILRIVSIIGFIGMHIGFGVCLNLYLFIFIPMVVCTAFIPSLLWDQFVIPFFEKKRENNIMTIYYNSQSQLLGKWLRFYNYFFLLTNRAEVVKYSKYDDESEIGWDEQPQKPIINSQITSTPHMSLSINGDHLYLEFDAVLLLFSKSYLLFPLAYIFNNSFSKFWYKFLMKNYFNHNNGNGSNNIIKDLLDKQEQEIQYFENDNINNRENNSNPKDQKQSFTTNFFIKLVCCLLMANILYYNIMMYRGLVDRPVVAYPINFEEYTRLLKIDQFWGMFAPEPPKSSKWVIIEGKFENFPNQFIDPHTNQPTNYDDIPFIYFSIGQRDRNYLLAIGSNEHLRLPYGRYICRKWNIYEKNEQRGRLISFKILTVLKGTPTPPWVKHTLLSNQINNNNDTLPTLVPIESEEPQQQQLDSNQPKIILLDENNNIKNEATPNNQNQDQDENIDYYLREVIHSHEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V9U0</accession>
    <name>A0A8J4V9U0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KANL3/Tex30 alpha/beta hydrolase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002209</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000059">
        <property type="protein sequence ID" value="KAF2076469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V9U0"/>
    <dbReference type="OrthoDB" id="10260961at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046879">
        <property type="entry name" value="KANL3/Tex30_Abhydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42103:SF2">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42103">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20408">
        <property type="entry name" value="Abhydrolase_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KANL3/Tex30 alpha/beta hydrolase-like" evidence="1">
        <location>
            <begin position="110"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="275" mass="30465" checksum="508FE1D7F794BDA5" modified="2022-05-25" version="1">MNTTDTTETPTSTTVLKLNKYKKVERRSIFLPVLNESSNIKLEGLLSWVDKNHVDEQQQNGDAVGGYSDTQLYDNVAVVITHPHPMLGGNYRNNVVLGVADYLTSYMRIPTLCFNFRGVGRSDGTGSWRGSTERTDTLSAVQYLLNSNPLLTQSGNNITKVIIVGYSYGSVIGSSVADEHDSIIGFTAISYPFGPLTLMLLGHLLEPALNSPKPKLFVTGDKDNFTGVPKFKTRMSEMKGTRVESKIFEGVDHFYQNSEKMLSKEIAAWIHTLCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VBV8</accession>
    <name>A0A8J4VBV8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA repair protein RAD51 homolog 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_000084</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000002">
        <property type="protein sequence ID" value="KAF2078584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VBV8"/>
    <dbReference type="OrthoDB" id="5957327at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033063">
        <property type="term" value="C:Rad51B-Rad51C-Rad51D-XRCC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033065">
        <property type="term" value="C:Rad51C-XRCC3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000707">
        <property type="term" value="P:meiotic DNA recombinase assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19492">
        <property type="entry name" value="Rad51C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016467">
        <property type="entry name" value="DNA_recomb/repair_RecA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052093">
        <property type="entry name" value="HR_Repair_Mediator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46239:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD51 HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46239">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD51 HOMOLOG 3 RAD51C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005856">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="9">
        <location>
            <begin position="60"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="23"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2078584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="330" mass="37147" checksum="33B1C6ABB4BCCBE1" modified="2022-05-25" version="1">MTTNDDDINNRINDDSDAVDQVTTTTTTTNNTTTSNTSSNTIKELNDGMSALDLLLKEGGQNRIITFCHDIDSTLNGGIALGKLTEFCGVPGVGKTQMAFQLAVNASIPKEFFGVAGKAVYIDSEGGYSCTRLREIATHLSNHLQEIASERSEKERDIAEQLSPDAILNNIFYYRVYHYLEQVALVNQLSSFLEINRDVRLIIVDSITYPFRKDFADMGLRTRLLLSMAQNLLSIAEKYNVAVVVMNQLTTKVSNNNNNSNNNNNQTILVPALGDAWTHICTYRMILYWKDRKRFCHLYKSPSFKSCHVPYQIDSMGIRGIEQQEEEEQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJG7</accession>
    <name>A0A8J4PJG7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010172</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000956">
        <property type="protein sequence ID" value="KAF2068502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJG7"/>
    <dbReference type="OrthoDB" id="10531547at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="150" mass="16692" checksum="A58AEB32F4DDBDCA" modified="2022-05-25" version="1">MTKGKKKINTATSNRVKKTVVEQKNDLDKQWSSFKRGDQLKNLLVKYTREYKEAGVVLRNEVGLQDALNLTVLGMKSINNGKTTTAANNNSKSGIAAKLLKLVKLTNMLINKESIPFKLIGDTKRIVFRDLDDITTTTTTTTTTTTTNNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJT4</accession>
    <name>A0A8J4PJT4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7743 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010461</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001129">
        <property type="protein sequence ID" value="KAF2068213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJT4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF29">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7743" evidence="1">
        <location>
            <begin position="383"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="592" mass="66100" checksum="D30BEFE17EEC6817" modified="2022-05-25" version="1" fragment="single">MVPSYPLDADYCINTAYYLIDLNGRTDIYSITGTTKFKNATHILQSAFELVNIGIMNHQHSLTFYLDPNFQTSFQLTNIATSNCEAVPYPLKIDLKHIPQTYNEVPMLNSLTYKPNLSPNIKIGAMDFKTKSGPFTTGSNILSNGYFQVSFSYDPLSNSSSTDQTITFSSLGNVTDVTLSSFLNQYYPPGNIGSVNFYPSCGADKKKSDTNIVLIETTGNPIYGSVSYGPYETFPIFPVSKVNGKSQYLSVHQNGAFSTSAVNITLGVYNKESPITSQCAWAPKPDSLPSFLSTNSSFYVVDQEKGLSLMNFIIKGAANIAFDDAIWFPQNSSYFGDFMGYGTEQNYTYTRSDYVLFQGPISGIRTILSGANFPTINFPTVFTDTQPPRLKNLEFEFLSSGWMLIRAHITDDISGFNKLTLSTNGAIITQRNLVSGTIYDGIYELRTQIFLTASSTIIFLLDRVGNLENINDFYLSNPFYSLEKQVPNLPAPVVELADITTFRFEPNNINVTTFGSLCTMYLGYKGAKPEFPPAIYMRLSKSFDINSETDMDRIPLMQWDSTLGLYKYQFFVPPRLFSGNIDYLLSLSSYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKS8</accession>
    <name>A0A8J4PKS8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Extracellular matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009971</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000869">
        <property type="protein sequence ID" value="KAF2068703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKS8"/>
    <dbReference type="OrthoDB" id="6406428at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052846">
        <property type="entry name" value="ECM-enzyme_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001673">
        <property type="entry name" value="S_mold_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31797:SF6">
        <property type="entry name" value="CHITIN-BINDING TYPE-2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31797">
        <property type="entry name" value="EXTRACELLULAR MATRIX PROTEIN A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00526">
        <property type="entry name" value="Dicty_CTDC"/>
        <property type="match status" value="22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035320648" description="Extracellular matrix protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="612" mass="66876" checksum="E286728CA00E0E11" modified="2022-05-25" version="1">MNNSLLFAFLLLLSSLFVVSNADWGNNNNVDIDISVSVCSVKSCGFLGCKQVELNCDDQDVCTNDVCGPKGCIHTPIVCDDKDCCTNDYCDKTKGCQSSPTSCDDNDCCTNDSCDKVKGCQHVAIVCNDNDACTNDYCDKVKGCQHVPMSCDDNDACTNDYCDKVKGCQHVPVKCDDNDYCTNDYCDKAKGCQNTPMSCDDNDACTNDSCDKAKGCQNTLVSCDDNDACTNDYCDKGKGCQTTPVSCDDKDCCTNDYCDKVKGCQHIPIKCDDNDCCTNDYCDKVKGCQSSPISCDDNDACTNDYCDKAKGCQHTLIKCDDNDACTNDYCDKGCQTTPISCDDNDACTNDSCDKVKGCQHTLVSCDDNDVCTNDSCDKIKGCQHILLSCDDNNACTNDYCDKVKGCQHTLISCDDNDACTLDYCDKLKGCQHAPINCDDGDATTIDFCDKIKGCQHTLISCDDKDACTNDLLDKVKGCTHSPVSCDDNDNCTKDSCDKAKGCQHVPIVCDDHDLCTKDSCDKVKGCQYEKLICDDFNPCTKDICTPKGCQYEAINCDDKDDCTKDYCDKMTGKCKHDCLYDEKDGGKCGDSIHIDTHVCFDNNDDDIDFDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL11</accession>
    <name>A0A8J4PL11_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000958">
        <property type="protein sequence ID" value="KAF2068494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL11"/>
    <dbReference type="OrthoDB" id="2559326at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="82" mass="9416" checksum="30E9B30099C1AC65" modified="2022-05-25" version="1">MNSLPSVFWGFVSLTAAGVGGWYISRKDYMEGKVKRVDEAVQKAKENQSSTITHKDQQVDTYVPYEQINNDLKYNPRRQSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL48</accession>
    <name>A0A8J4PL48_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009821</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000819">
        <property type="protein sequence ID" value="KAF2068855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL48"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="131" mass="14750" checksum="2780B2F74F2C0475" modified="2022-05-25" version="1" fragment="single">VQGQIKSFSHKNELMIGFFIISLVKEYFIEFNQSYLYNHYIKIHEQISFSSQSSQAYPSSATSTPLFTTKKQKLNNGTTIISNAPDQQVSLSAATNIEFDSAVAASSRNKPSIQSLESYFDHLCQLIDNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL51</accession>
    <name>A0A8J4PL51_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008804</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000580">
        <property type="protein sequence ID" value="KAF2069882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL51"/>
    <dbReference type="OrthoDB" id="19610at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48146">
        <property type="entry name" value="K-STIMULATED PYROPHOSPHATE-ENERGIZED SODIUM PUMP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48146:SF2">
        <property type="entry name" value="K-STIMULATED PYROPHOSPHATE-ENERGIZED SODIUM PUMP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="342"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="342"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="366" mass="43805" checksum="E83E451BE8B0C208" modified="2022-05-25" version="1" fragment="single">SKRLSSSYYPYPCLDDNNDQTPIYYYQLIAQYFKNNPDEIDSFRPLLYSLWTNNQWFYLIYSSMFYLWLLEYRLSLTPQLNIFIKASNRLFWFDTDHSTKKYYEVYITIKNKLLDQSLWIGLCDNQNNNSNSQSSNSNNTNTTHLLRSRRIWVDFFHIVSIFYFYYEPNNDGLEEFRRNLNSQYNEYILDTIQKNGTLDSNVLLSIDDIFIRGIIRVLSLIKHEETLIQYIEKCTIFKEGWNMNPTTRVKLQSCLYSLTKPGSPAYVPRSVRSTSRKVLDILFPAGKLSRKTVNLFFRLLNPYYSIGSIFHWVYDTVKYYLPFLNNNNNNNNNNNNQIQINNNNNNNLNQNNNNKYNNIRRREINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLH1</accession>
    <name>A0A8J4PLH1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009612</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000757">
        <property type="protein sequence ID" value="KAF2069065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLH1"/>
    <dbReference type="OrthoDB" id="2414723at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045068">
        <property type="entry name" value="BACURD1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING ADAPTER FOR CUL3-MEDIATED RHOA DEGRADATION PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145:SF8">
        <property type="entry name" value="RE57120P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="71"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="157"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="306" mass="36161" checksum="0F78B210C0F605CB" modified="2022-05-25" version="1">MQEVPQENRIDEIGDDQDNETYNSILDSFNDAQIQFKNDFKLKKEFIKTLTEKKSKLLEIKKVMDNSIFPEHVLLNVGGMEYRVKKDLLTIPGSYFDLMFSGEVDIKPLPNNPHLYFIDRDGIEFRNVLEYLKNRSQDPFIDLNLMRELKFYNVEGFSLSTIMKDKKLDVIASWISKEKVSFKLIFKASENEFKASEFHRKCDYQGPTLILVRTKYEVFGIYVNDFWYRMGTDFGGSESFFIDRKMVKHQKILDVAEIEDKNSCSFLKLGFQISDRCNENWSSFYTKDGFKDTFRPIEYEVFTVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLZ3</accession>
    <name>A0A8J4PLZ3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Clu domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_008232</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000490">
        <property type="protein sequence ID" value="KAF2070452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="550575at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.950.10">
        <property type="entry name" value="Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033646">
        <property type="entry name" value="CLU-central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025697">
        <property type="entry name" value="CLU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027523">
        <property type="entry name" value="CLU_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003128">
        <property type="entry name" value="Villin_headpiece"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036886">
        <property type="entry name" value="Villin_headpiece_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT EIF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601:SF20">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13236">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12807">
        <property type="entry name" value="eIF3_p135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02209">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00153">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47050">
        <property type="entry name" value="VHP, Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51823">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51089">
        <property type="entry name" value="HP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Clu" evidence="4">
        <location>
            <begin position="193"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="HP" evidence="3">
        <location>
            <begin position="1915"/>
            <end position="1978"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="85"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="142"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="520"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1231"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="527"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1231"/>
            <end position="1244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2070452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1978" mass="224591" checksum="D30315633CD407C4" modified="2022-05-25" version="1">MYSKDKVYIYDVYSYFGDGSTTSSNGLNNNSNNTPPILEPNLLSYQEDVPSQTTTTTATTTTPSKPFQHKVIHITDLKASANNINTGYSNNNDSNNTGYSNNNSSNNESNKISYSNSNNISYKDIPISSYQIHEKTINYTPDISSPPGINYVENVDDNDLDNCNNNNSNNNNNSSNNINSSPNLHFNTKLNISLNNKSNNSLLADQLNRYATGEFKGKDWNGEFQNLLSQSNSEQKFRKLSHLAKDFVFTAKSYATIIINELCIPIELKTIKPVDMGGFAGGLKYHCQGILFKFAMDTRLNHDCWMYGGKTARDDYAAKAASNELKGLISYYMTDVKGLNYPLMALIDYKGFRLIALSVLPISSDTIKYGSSDSGSVVHSEIPELNDKMKQVGEKLNLKAHRVGSIPENTKILSSPGDIEAHKGFDNRYYVIDFSRTFPPESISKQPNINKRCIFYNLLRPEFVSKWKKPLCSDAFSGWQRNDSEKSIHNKEIEEATYYLVNESVRSLANKFDQEKNGITNKLQSQSNNIINSSSNNNSSSNSNNSNNNNNNNNNSNNNNNNNNNLSTTPLQKVEEPSDFNQIKATFAVKFFPILQKRFEIGIPTVSDTLSGNTLGEIQSSINLLGSLDSTTSSSLSNNNNNNNNNSSTINSNINNSSISIFTNSIVGNSNATPTSPTTAITTTTTKDTSIDLKNKYRPLNQVKNEVPQQLLRIIEEMHRNGINIRHMGRVRYYTKSKTIRDLLLLEMTARSIKNIIKDEMRRKMKDEKGLSEEPFKEVVIEIFNQVLDQSSSFWIKVLDCIRVKYFMAFMKYDETLDQQQQVVPSLRSSSSSILSSTSSHYPIEEVDEQTWMENINIKNLLDRLQILTGISFSPGAQAEFSRDPFRFQFLDSDILEISPNVKHMNIIDYAEGMALLMSSLAKKGREKLRLLKMATQKFQISLKSNPDNFECLCQLGRTLGMQAETLALTTAIFGKNIYLNALEEATLKFIDALSINPKFSKAHYELAHVYILIAQYYSNNLYKSDKNYRLAAYHLGESITTFKQQQQQQTNSLSTSLSSSTTMADNEFYQTIFNDLTLLFFKGIEGASSNILGVSILCEELEKLCPDDPNLLILFGKSYIYRPFYDDPHHSKNNNNSSSFLKEYYYEEATKKFHKALLINNTLTNVVLEVGNDIWLKSKIGNRKLYYPAASIFKLLVNGNFCRENRVFSMYTDSLMEILKMEFSQPSPSSFFNNNNSNNSNDSSKNKNKQKNNIPIIITPISVDQQQTYKSIYLNETILLFEYLFSINNRYAIDKLESAFENQQGELCDFIKMANHSNIIKTKLLEYSKGVTKLSLKRSHVHDDDLNVIGQLFAQIVELDLSYCPLVSDQGLSDFLSNFGKQLITLILIGDLVGQKTISIISQFCQQIQYLDLNNCNLLDPESLVSLNELQKLKTLDLSKCRLTNETIQSWNKLNSLQTLRIRNQIRVTDECFYQFQSWNTLKVLDLSSCSKISETIFQYLPPKCLNLQEISLEACYHISDTTVISISNRMPNLVKLSLKNCKSITDESIRVLVQQCKSIKDLKLSRCHSLTDESVKLITENLHSTLERIDFSMCPEIKEESFINLLTKCTNIISVNLSENPKVSDRTIETINQHLPKIQHLKLDSCTKISVDGLNLTNTLEIQTLSIKKSQICHHTLCDLSITLLNLTSLTLKACLQLTDHSFAVIGQLSKLEYLDISDNYRLLDNSMILICKNLTKLKHLDISSCLRLTTKTFFLIGKFLSKIETLILTGCGNLTDSSLTYISENLLCLLSIDISGCLLITDKSIYSIANNQIHLESISLKDCKNISQNCIDYLKTKCPLFKIVRLSLHSMPLMGELPKSPASMLKETPQQFNPIKAAEWKQLEEDRRRQAEAKAKVQKKQDDEFEKLHRYEPFTTKYSFDFITSPNKNQKLDPSNLEKYLSDQDFVKYFQMKIESFESLPKWKRDDKKKSIGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM75</accession>
    <name>A0A8J4PM75_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mitochondrial cell division protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_009897</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FtsZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000846">
        <property type="protein sequence ID" value="KAF2068781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM75"/>
    <dbReference type="OrthoDB" id="70257at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048285">
        <property type="term" value="P:organelle fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02201">
        <property type="entry name" value="FtsZ_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000001">
        <property type="entry name" value="Cell division protein FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00909">
        <property type="entry name" value="FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000158">
        <property type="entry name" value="Cell_div_FtsZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020805">
        <property type="entry name" value="Cell_div_FtsZ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045061">
        <property type="entry name" value="FtsZ/CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024757">
        <property type="entry name" value="FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00065">
        <property type="entry name" value="ftsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314">
        <property type="entry name" value="CELL DIVISION PROTEIN FTSZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314:SF4">
        <property type="entry name" value="MITOCHONDRIAL DIVISION PROTEIN FSZB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12327">
        <property type="entry name" value="FtsZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00423">
        <property type="entry name" value="CELLDVISFTSZ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01134">
        <property type="entry name" value="FTSZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01135">
        <property type="entry name" value="FTSZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="4">
        <location>
            <begin position="91"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="5">
        <location>
            <begin position="287"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2068781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="395" mass="42968" checksum="8938B937F9AD5ACF" modified="2022-05-25" version="1">MLKAINRIEGLLRYHHSKLYRCSLLLSSSCALVSTNSSTSTLPSATTSSLKQSEPCIGFYNLDFSNQYIRPFSTTTINHNPRDDLEIFLPKISVVGVGGGGGNAINHMIEKNLGGVNFYACNTDHQDLIKSLSKNRIQLGAKLTKGHGAGANPEKGRLAAEESKQEIIDNFKDTDLLFLAAGMGGGTGTGSSPIIAKTIKESKKDIIIVAVVTIPFKFEGKRKHLIAQQGLEELRKYVDTLVVISNENLLGNTNRDTPLDQAFLFADDILHNGVRSIANIINVPGIINLDYSDISSILIGRKGLSRIGFGEASGQDRAYKAVYDAMNNPLIENDNEKFTGLLVNISGGSDISLKEINRVTVYLQENSDPDVQIFVGHCVDESLLGSMRISVLFVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMM8</accession>
    <name>A0A8J4PMM8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008713</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000565">
        <property type="protein sequence ID" value="KAF2069963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMM8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="240" mass="26528" checksum="85AD9ACEFB2125E3" modified="2022-05-25" version="1">MLGGTYYNFNFSTSQYPQLHFSTLNISISPLITDNNSLNVTLSGQYFQTKIINIISNLFSSDSIISNSIFNSNSAESSGGAINVYLGQASIISNVFENNVASYGMTISAYVTTIIIRDLSGDNYFSIISCSSSTFSLLSNTTLTTLNHDKSGKCSSCYYYSDHNDKNNLGYCQTAPPTKHLSSQGKLMIALIIICSLSLLAGVIFLVRNMRKSNPYSDYNEIHDKHRFIPFVLKNNDNNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMQ0</accession>
    <name>A0A8J4PMQ0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008616</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000549">
        <property type="protein sequence ID" value="KAF2070059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMQ0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="447"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="451"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="620" mass="72009" checksum="4BCD368406FAE2FB" modified="2022-05-25" version="1">MNQITSNIVYSTIIDHYFQQYLQLVCSKDLALKSKHRSHFSCEIDGDQEIYNFRTIYSVVVHLAKVCKQWRDLLVRKRYPKCFVERGDLVHFIATNQRHNYMFRIDSFAWTDVDGLTSDGVDKQQLYDLVSLDRPCDSYSLEQVQAAVSLVHDNTFVFNSVNEVAHYEHLEQVLSMCFADIKNNNGNTMLKRIATRYIDGPRVAQIIQRLLNKYETIDELYLLGIARLPYDGRDGSFCVPSHVQIKKMWIRDFSDYITLDAFQHCTSLQCLSVDFDIFNVSSIIHHPSLTSLAVSTSTLDKNDVIQYLNTNKTTTQLYIEFSDIENNNTKHPISNTTLKKLEVVSKSSSILHLLELWNTDSAIEEFKMKFALSPHIFSKHPNIRHLDVNTIIKIPNQDFHFPFLNNLVSLKLKYYGDKLPSWMDQLFGRVDQNRCNKGGPLNPVLKRIVDNGSSPQKDSNNSNKNIQPNEYYSTPRSKGRALHYQSLLVDYERDGDIPPFSPVDFLQNNNSNNSNNENLTPNLTSLSIDFYPDWNWEFIQLRPNITSFNIYLPSFLTNISQIHTFLNYNPNLNQFSLSNLHDQVTFDLVKQEIADFILFETNLTSFYIGKDNRDFYNLFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN07</accession>
    <name>A0A8J4PN07_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">protein xylosyltransferase</fullName>
            <ecNumber evidence="6">2.4.2.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Peptide O-xylosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">CYY_008729</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>UDP-alpha-D-xylose + L-seryl-[protein] = 3-O-(beta-D-xylosyl)-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50192"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:12567"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57632"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132085"/>
            <dbReference type="EC" id="2.4.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycan metabolism; chondroitin sulfate biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Glycan metabolism; heparan sulfate biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the glycosyltransferase 14 family. XylT subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.26" evidence="6"/>
    <dbReference type="EMBL" id="AJWJ01000567">
        <property type="protein sequence ID" value="KAF2069952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN07"/>
    <dbReference type="OrthoDB" id="2019572at2759"/>
    <dbReference type="UniPathway" id="UPA00755"/>
    <dbReference type="UniPathway" id="UPA00756"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030158">
        <property type="term" value="F:protein xylosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050650">
        <property type="term" value="P:chondroitin sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015012">
        <property type="term" value="P:heparan sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003406">
        <property type="entry name" value="Glyco_trans_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043538">
        <property type="entry name" value="XYLT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46025">
        <property type="entry name" value="XYLOSYLTRANSFERASE OXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46025:SF3">
        <property type="entry name" value="XYLOSYLTRANSFERASE OXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02485">
        <property type="entry name" value="Branch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="14" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="84"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAF2069952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="598" mass="70848" checksum="A7A826E6D4F0B9E1" modified="2022-05-25" version="1">MPKKILLLLGIIFLSAFISFSTYISLFRDNQRIFNHEIIKTTELQNNNFNINRLMNNPKSVDISNSGATDNNNNNFIYFNQNQNQIKSTPPPQHENYLKKSRPPRPPPLFKREKKVEQKLDDLVPKTKYSTSMLTKECLNGGIGFQDKCICSFNYHGDQCQHMGEDRCSNPKFHFEDDKHIQHTTGLETFKNATVPFCCWSKHRHQTNLYRNKLRDNQIDSHHDQLKQILTNYGPWNENDKVFLNHLFSQADPYSADDSTHQYKLIMQQDRKKDKFPVSKSRKSHLAFVLLFHRIEMDEVQQLLKYIYRSKHYYVIHIDKHFNDTAKLDQLNNYINQLYENQLNQNSLLPSNYPKNIKLLDSRYYGAWGSISLVYSELSAYKTLFNMVDERNQAINQHQPWSHVINLSINDFPTQPILALEKFLATESSLNTNFLNEDIPKESSRINKPWLECNRKLYIFNEQENENVCGGESYQQLYDSLDHTAYTDGAQWHFLTNKFARYLISNIKSLERLFSLKFSLIPDETFFQVAKSESYLFTDSEYSMKWDQYNYRYIPWSNLHYEVGLDDISKPFPYETYFTRKVYSTQVKNAIVEKYLKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN64</accession>
    <name>A0A8J4PN64_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BRO1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_008367</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000509">
        <property type="protein sequence ID" value="KAF2070314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN64"/>
    <dbReference type="OrthoDB" id="64867at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09246">
        <property type="entry name" value="BRO1_Alix_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.560">
        <property type="entry name" value="alix/aip1 in complex with the ypdl late domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.50">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.280">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025304">
        <property type="entry name" value="ALIX_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030">
        <property type="entry name" value="PCD6 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030:SF30">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13949">
        <property type="entry name" value="ALIX_LYPXL_bnd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03097">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01041">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51180">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRO1" evidence="7">
        <location>
            <begin position="1"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="697"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="707"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="732"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="773"/>
            <end position="790"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2070314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="805" mass="91397" checksum="0523C0BE57A96F4E" modified="2022-05-25" version="1">MLSIEKKRTDKIDIVKPLTKYIKEQFSKSEADAHETAIQTLNSMREDIRNLQDKTETSKDMLWKYYSILSSLEIRFPIGESNVRISFPWTDSYRQRKTSLYSIYFERSCILFNYGSIISQIGASQSRSNVEGIKKACNSFQCAAGVFNSLREYISMHPECFASPDFSSESLGMIVALMIAQAQECIYEKAAMDNLSESIQSKLAAQVAENYETVDQLLNSATLKTLVDRNWSSTILIKVFLFKSIASYKHAKGLETISAFGEQVARLNSATDSINSAKANIQRFTPTELRDTVEKYLVTIIKYRDFAKKDNDTIYHDMVPPEHKLTPIEKKSIAKSIPLPEISFVDPFNNLVPYSVKEDAAYYNDQKEALLKKEVGNIDFNNQSAKASLLSMGLPGSIEALEVGIPKALQEKMDIVSSEHGVSNITSLLENLQLMSEEDSAICLAAGSILKKEEDEDNEMRNKYQASWHRTPSYTLTANLNQDLAKYQMHLQHSTKSDGFIRKKFEDNKQLIGELEHQDQVISLLPSNMLPSSKIPEIASLTVLLNDLDSLMATREQIAEKLKDLSKKDDITLKLIAPTRDKSVIYAEEIKKYEPLQISLNESFLKQQQIIENIRRENEKFTNSKSSQGGEREEILQRYANAFKIYQELKSNLDEGIQFYANFQEILVKFRTRCEDFANEREKERIELVRQISAGVNPYSPAPYTPSPNSSSGGYSPYGEAHQSPVMQQHHQPPPLGPPQSFRPPPYIHGAPQQPMMPPPHQYQYHHQVQMNQPPPPYQQSFAAPPPSFSVPPQIGKMPPQYKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNE7</accession>
    <name>A0A8J4PNE7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_008412</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNase II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000516">
        <property type="protein sequence ID" value="KAF2070265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNE7"/>
    <dbReference type="OrthoDB" id="24007at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004531">
        <property type="term" value="F:deoxyribonuclease II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004947">
        <property type="entry name" value="DNase_II"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03265">
        <property type="entry name" value="DNase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035267098" evidence="4">
        <location>
            <begin position="26"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="496"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="574"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="582"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2070265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="658" mass="74631" checksum="7F7703FEAD885EB9" modified="2022-05-25" version="1">MKECKILLVLIVVLLLSLSVNNVSSQTCMSDELLTGTSKEVDWWFIIKIRGFSDVYYYYDSDMDKKNEANFKIGYFLRSKFSAFGATLLPYANQDGNGVPITTLKTNFIAYNDHFTLVTKSSGKKFSDNTFYDKGAHEKGLIGWSKTDPGKGLIIQHSLPNFPYGYERGMFQPVMNDNIKDLNGKQDIKGQEIKMYFTMYGWKTNIFGPNLMPYHIPGRKFYVTPEEAKPNVHGHGTRVQTGALQLSNKIAYTTTSQYELFSLLFTTTVKPSQQIFCSSLVDEDKKTSPGTKVKEMVQFMSDLSDGGLYAGKFNEIETSLTEYVSYEKQPLVKVRPSRPVFSEKEIKVGEYNYYMRINHQNDEGIDGKGKVDDIWKSLIDSRKEPNLVITDQDTSQLNNLAISTWAYSKPDSWWKGKIVSPVFKINAHDNTFTWKGNSNQEHSKIAFRTKDIDSAKWNVCSSGGNLYSTGKEKIKSSLLICFKANGLRQALQNIMTNDGDKDDAEISKDDGEDDENEVQDAKKPDKFTQKLSFIKTLTGTFKDRLSTNLEHAKQMSQEMDRLATKGRYFFNFDPSKQKDYRDSDDDDDDDDNGLNLRRSPRLQGKSTATAQTAKKKARPTGKISPSPFVSLSMPPSDVDLLQDKIDQTVPSNIFYTIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNN0</accession>
    <name>A0A8J4PNN0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Delta-aminolevulinic acid dehydratase</fullName>
            <ecNumber evidence="3 13">4.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_007488</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 13">
            <text>2 5-aminolevulinate = porphobilinogen + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="4.2.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the ALAD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.24" evidence="3 13"/>
    <dbReference type="EMBL" id="AJWJ01000402">
        <property type="protein sequence ID" value="KAF2071202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNN0"/>
    <dbReference type="OrthoDB" id="1530at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00318"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004655">
        <property type="term" value="F:porphobilinogen synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000019">
        <property type="entry name" value="Delta-aminolevulinic acid dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001731">
        <property type="entry name" value="ALAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030656">
        <property type="entry name" value="ALAD_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006762">
        <property type="entry name" value="PRK09283.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458">
        <property type="entry name" value="DELTA-AMINOLEVULINIC ACID DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458:SF0">
        <property type="entry name" value="DELTA-AMINOLEVULINIC ACID DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00490">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001415">
        <property type="entry name" value="Porphbilin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00144">
        <property type="entry name" value="DALDHYDRTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01004">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00169">
        <property type="entry name" value="D_ALA_DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="6 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 13" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="10">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="10">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2071202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="333" mass="36600" checksum="5AF825FCB6934184" modified="2022-05-25" version="1">MYPSQPYNHYLHSGYANPVTKSWQGDSTILKSQLIYPIFVTDAVNTKTEIKSLPGQYQISTDLVIEFMKPLVEKGLKSIILFGVITNTESKNPQATSATDKNISPVIKSIELIKKEYPDICICTDVCLCAYTNHGHCGILTSDNLIDNEKSIKRLGELSLSFALAGAHVIAPSDMMDCRIAEIKRVLFEAGIGSRVAVMSYSSKFASCFYGPFRDAAGSGAKFGDRSAYQLPAPSRQLAVRAALRDQEEGADFVMVKPAGPYLDIIREVKDAVRVPVCCYQVSGEYAMLWHAAVAGGIDLKSGVIESLISLKRAGCDIFITYYTPQLLEWLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNU5</accession>
    <name>A0A8J4PNU5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008777</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000576">
        <property type="protein sequence ID" value="KAF2069906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNU5"/>
    <dbReference type="OrthoDB" id="10653447at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="192" mass="21941" checksum="B0B254B7DA2B4FCB" modified="2022-05-25" version="1">MEAYLPILQNGNLVDFQNPLSQFLFIKLPLIYKDYSIATSVLIISLVTLNTLFIAFLLLKPNSYSPQSKEMIVRENNNNNNNKTPTKKKQKHNNNNIYHSPKVSNMKLQSPMKLPSQESPTKMNHLESIANLYPSPEKPKSRKSLVKTPSKTKSFLNIDNSPPANPTRRVSQPPTRLIDVLNEEKNKTKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP59</accession>
    <name>A0A8J4PP59_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007078</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000358">
        <property type="protein sequence ID" value="KAF2071607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP59"/>
    <dbReference type="OrthoDB" id="22074at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32142:SF62">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32142">
        <property type="entry name" value="B BOX-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="722" mass="85253" checksum="E0680BFD65EA4FBC" modified="2022-05-25" version="1">MENNNNNINQFFRYIYNNKFIKTRIFSFLKHSDKTSLKYDDICDLRDIIAHRYWSLLRDKIKRNEYLYLNRNSCELIFKIKDLDLFIKVFQRFKDYFPTLPPKIQYAALYDNIDVLKYLLNDSQHFIDIFKAFQWSFENKNYQMVHYLIDFLEQDKRMVSVLNRFGSPLISKVILLSISSKDIETLKRIFNFQAPKGVSDINIDEQEMFKKAYSTGDLQAFKLVHQKYSNNYNSILKTILNPHIRLCLGNNIKYRKDTYKKMTLQYDLLEYLLDQNLVRPDVYQDLKNCLVFQAFKLQDMDLYNKLMNQSNTKAICFNELSLSMITGSLSSLDKVKELIGLGVTITTTCITNSISSLNKNKKDGWEIFLYLVENHKMDYDIRALISMACKTANIKVLKFALDQKVEIKPQPWWVGDLKFSDENREFFQLLFENFRFESYILFGATEKSIQEGSIENFKTLLSMIQKNNNQSEFNRLYYRAASQGKIEFLEILIQNRVPHDHIVLCRASSVEIVNLLYHSGLAKSNPNVSYNNPEPLSIEVLQHLASLELPDKDDLMLCLLRNLIENHRIYSFQLLLSHCDFENNIDMVQNILICLATNNNLEMIQIFLDYQLNTTSTNYVPCFNVAMSNNNLELVEFLVDIFSIDYLVRKEDTRDFLQRLQDTKPFINNYFQSIFLDPQNQQFLESRPDLLKMFSSQPTNDIVDVDLYFKYLESNYPTKQST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPN5</accession>
    <name>A0A8J4PPN5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BEACH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_006757</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000326">
        <property type="protein sequence ID" value="KAF2071920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="26681at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06071">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01201">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1540.10">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036372">
        <property type="entry name" value="BEACH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050865">
        <property type="entry name" value="BEACH_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046851">
        <property type="entry name" value="NBCH_WD40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031570">
        <property type="entry name" value="NBEA/BDCP_DUF4704"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023362">
        <property type="entry name" value="PH-BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743:SF108">
        <property type="entry name" value="BEACH DOMAIN-CONTAINING PROTEIN LVSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743">
        <property type="entry name" value="BEIGE/BEACH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02138">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15787">
        <property type="entry name" value="DUF4704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13385">
        <property type="entry name" value="Laminin_G_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20426">
        <property type="entry name" value="NBCH_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14844">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01026">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81837">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51783">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BEACH-type PH" evidence="6">
        <location>
            <begin position="1125"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="domain" description="BEACH" evidence="5">
        <location>
            <begin position="1247"/>
            <end position="1535"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1698"/>
            <end position="1730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="847"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1037"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1907"/>
            <end position="1929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="847"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="865"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1049"/>
            <end position="1122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2071920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2009" mass="229297" checksum="72D283CD65F85C32" modified="2022-05-25" version="1">MSTLGGDQTPFNMYSKALEIESTSQPLYRNEEIYFQEVLSKIKNSKSPQFSEFNIVFDKLINLSYQDIYSLKKVNFDYFHMFFNLLLESSILSVTESSSNRNLHLLLLKKLNALLYSSALIQNYCCNDKWITKLLNYLISSSKHDSDIIGEVSVCLQILCYFNITPKEFKFFIKLLESINDERPYWWNVLVEVLEYMFKKRDGPDTFFNFSNASSGLMVPQRQAFDGGYSISFWINTEDFTNFKFKPSLYSFFSDDDVGFECCFYLQSLVFSIKTKTKAPIIKSVYKFQPHKWYHVTIAHEYFLLRKSQLSLYVNGRLEEKMPLLYPKSDKPFTRCHVGNSMSMTNAFIGRMGSILMVQEAFTPVEAAHIYSLGKDATLIYEKIPREGTTGIFNGELFLETGKRNIKIIFLYHPKATDKALCFEISTGELPNASTIMEGVSIIKTTSPIDQLINIGGIKMIYPLFGQIGQNISGMQIEAAEINELTSIACGDVDFVSIPHSHAPTPIFPSSLGSGHIGSLLKVLLSLLEHQPIFREQIIETHGFQTISMLLKSMPSSAPYWTPEDIDILGRLISFTSTNLQLWQIATQHLLLNNFQLWCETHSLTQICLFETIRQRVQNNTHFWRNNVRVDQWLQILKKYYVLPSQKEQQLINSSPKVSTLEIYRDVSKLERIKKSRSQIIVILRETSSPMFSQAESRWIALYLKESTLENQEDVVRMLEDMIPPQQQNTDTWWEDVLSSVSAYDENLINKSRNDFLSVQMKLPKHISYLWDIDCGEEIMKRNNDKFLEKRNQYLLFKNWIKIRRNRAGNLNGIKIQNINVNNNIFNSIFYKLKELGIDYNNNDNSNLNNNSNNNNNSMKQTPTSPNESPVISPSSSPPKNNIGIKTTTTTTTTTTTNIVEPEDTRTIFWKLDKTEGPSRMRRKLKRNYLGSDYRGLNKQNRFGRNRRIIADTFETDNQTYYIDQDCGDGLGNLVFSIQSDKLISPNTTLFKEMEFLKELQNSNAVISNSSNILFHQLSISGGNYLLNSSTISNNSNTITTNGGPEFNSSSSSPTFLKSPSKQNIFSNSSTPSTTNNSTPNSSLTNSSNNSLNNNNILSPNNNQNNQNNNQNNNPDNNQNNNIEVEPEKLIGLYNCLLITPVGAINGKLTLTNYYLTFDKEIKYDKAGVKIPDPDYGSIVIKNTFNWRIRDLVEIHKARYLLRWNSIEIFLNHKSYMLSFSTENESNVIFNKIVSLHPPNLKVKWSEHPSKIIKKTKLVMKWKNREISNFEYLMALNTIAGRTYNDISQYPIFPQIIADYKSSTLDLKDASSFRDLTKPIGALNQQRLDTLLARYNSFNDPQMPAFLYGSHYSNFGIVAYYQVRLEPFTSCHLSLQSGVFDHPQRMFESMPRMWEGVTSANLADVKELIPEFFYMPEFITNINNFNFNFQTSPAGDLILPPWANDSSEEFIRKNREALESEYVSANINHWIDLIFGYKQNGVHSVEANNVFFHLTYENNQALQRDDPDERNSINSQIREFGQTPSQIFTKPHPKRKTLEELQKTPKDLFSRITNLFPSSNNNNNNNNNIDIGTSPTLSSSTQYPFKILKTNSKLPLVHIGSCQDSEIIVLVYRDGVLAVNQFDPSPSNGLPFTFDIDKSLSTYREKQIDTLFMSDSVTCISNCFILTPDGKYMFSCATWDSIFKCSNIQNGKIHRMYRNFHQGMVTCMGLGSSGKTLATGSSDTTILVWQDCASLLRDSRAKPSYRLCSHDEAVQCLDISEEWDLIASGSQDRKCILHNLRKGTFIRSMVHRGAVEMVKISTVCQSVITYCSMSFLYIHSFNGKLLAMVPCDEKLYDIKLTGEVVVKKGGVFGVGDSNQYIITGGAKGVKIRSLPDLSIVHSFDSPTTIKSIALVAQEKFLMIGLNDDSEDNEDRRDDQGEEEDTDNSKAKVFSSKIAEILKNNSNFESWIAEYISQSLGFVEAVNNRNNNIDPEWATIAVEKHISGLCKYYFEGKTADRQGIHLSSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPN9</accession>
    <name>A0A8J4PPN9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006878</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000336">
        <property type="protein sequence ID" value="KAF2071817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPN9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="89" mass="9949" checksum="96C5EF1C9B626BB9" modified="2022-05-25" version="1">MTIFENLKKLHYSNNGDVHASVFATNHLSQLNSTTSFNMEARLSVNLMSLGNPGSRIGTGPTNTAPQAWDLYFSRYLQPQQQSWGVGFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ28</accession>
    <name>A0A8J4PQ28_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_008655</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000555">
        <property type="protein sequence ID" value="KAF2070024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ28"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07091">
        <property type="entry name" value="ALDH_F1-2_Ald2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000050">
        <property type="entry name" value="Aldehyde dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000026">
        <property type="entry name" value="Aldehyde dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000001">
        <property type="entry name" value="Mitochondrial aldehyde dehydrogenase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="5">
        <location>
            <begin position="19"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2070024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="494" mass="54244" checksum="B47BC08661FD470B" modified="2022-05-25" version="1">MEVPKIKLPNTKLFINNEWVDPVVPKTFKAISPITEQVICEISEGDAADVDKAVKVARDCFDNVWTNYTGEERGRLLYKLADLIDEEKEYIAQLESLDNGKPIKDSFGYDVFSSSQTLRYYAGWADKVQGKQIPLGEGFTSYTRHEPIGVVGFITPWNFPLMILAWKFGPAIAAGCTIVAKPSEETSLTALYFAELVKKAGFPPGVFNLICGYGVTVGNAISGHMGIDKVSFTGSTRTGKTIMASAAQSNLKKVTLELGGKSPNIIFEDADLEFAAIGAKAAMYFNMGQSCIAGSRLFVQESIYDKFLEIFTAKVKELKLGNPYDKSTEQGSLASKIHFDRVMSYINKGKEEGATIHLGGGRYGDKGYFVEPTIFTNVKDSMTICKEEIFGPVICILPFKNVDEVLKRANDTEYGLAAGVFTKDINLALQVGNKLKAGSVWINQYNMVNIQAPFGGYKQSGIGKDLGEYAIIDYCAIKTVAVSHRTFQAHTHPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ37</accession>
    <name>A0A8J4PQ37_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006574</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000310">
        <property type="protein sequence ID" value="KAF2072100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ37"/>
    <dbReference type="OrthoDB" id="24030at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35885:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35885">
        <property type="entry name" value="CARBOHYDRATE BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035287329" evidence="1">
        <location>
            <begin position="21"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="308" mass="34407" checksum="5B2AB0BFCF59900A" modified="2022-05-25" version="1">MKFIITLFVLLSVAISYTRADTYSVGIWNNGTNINVGLIDLTNGMEAQLKLVIPGAVFPSNSIQPSTYNYATKFLSFYGLQSKTSPYLYVVDCNSWTLVSKSSYSYLNIYSGLASTGTTANNLFTTYSAGGVLSVARIDPSSKIVYKFDSFYGNYRGSVYVPSISSYYVAYTNQTGLYIRAYNSNYQLAFTGQFGFANNPYPINDYPLNLVYSPLNQAVMAQVYMTDSNNRPYYALAYLDWNAGLFHVTNMDGYTGDQFLSTLPDTLGQQLAYSFAYSFGQYVIYTFNTASNVFLTYRPYLTPVLSAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ85</accession>
    <name>A0A8J4PQ85_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000356">
        <property type="protein sequence ID" value="KAF2071633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ85"/>
    <dbReference type="OrthoDB" id="18128at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000120">
        <property type="term" value="C:RNA polymerase I transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039495">
        <property type="entry name" value="TAF1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14929">
        <property type="entry name" value="TAF1_subA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="439"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="502"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="528" mass="63135" checksum="C587E0255DFF5B75" modified="2022-05-25" version="1">MIAEEETLLGGQHNQIHHHNNNDREWLNIQKTFITLVLQHRNEEAVTFISDILKTTPIKLYLIPSILNLLLQYDFKLYSSQLKPYLFYTGFFLCNFSNIKEVPHQLLYYLFTNEIYFRNFEEALGRYTGFISNKNEDSYPLLIVYGGLLYFFQWDSKLKEFNKKVSRMKKKEQQETMATIKQCCENAESRLRKAHSLFPQYDVILKPLIQILVHIDKIDECESILSQNVQKYPLNPIGYNLFGSFLMQYRPFKFKSIIACYRHLFTLDPVSNVAFGVLNQIYYLQKQKLADYGLKFKYMLQLYMERLSYLYNDIGLWRMLFFFIKDHCVREHTVLKKYLDNTNNRAKLELFYNRHYQPFTDFQSNTTLMRFKAGTYYYLSSEDALDYINDCLILRQDFQFSLSILQFLQSDCVIKVYNGKEERKLKNFDKLIRTSQKRFGKNNNNIDDKKDNDEKADKKTKMTKSDPIKKEKKENDDKITPTKNNDNDNEKENDNGESLSVGKRKYTPKKKMKNIKRIENFEKKRKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQD0</accession>
    <name>A0A8J4PQD0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007499</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000404">
        <property type="protein sequence ID" value="KAF2071182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQD0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="88" mass="9939" checksum="EAEEE3E3B1D58B66" modified="2022-05-25" version="1">MGKHKKEKERELEQEAMHSGSMQQQQPYGGSMQQQQPYGCGGMQEQPQFGGNGGQQLPHPHDKHPTLHKIESFIPGTDQHKIAKDIKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQQ9</accession>
    <name>A0A8J4PQQ9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007594</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000414">
        <property type="protein sequence ID" value="KAF2071090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQQ9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="318" mass="37512" checksum="6C467CFDE33F507C" modified="2022-05-25" version="1">MSNELSTIDDILALLYQLKQSRGTDTCDVFSSIQNKLESINNYQPTSTKIEKQKELNKQSLSHLQLLETKLENLYDQYDQDTFSNLIWIPKDIDVNNMSVNDTNENNDNNQEQIDKPLMKKELYKLSSQYISLVDKYNSKSKDILHKKSKDINSTNDNDAILLKWYKFILKEINDQLSCNNVNNNDNNDNTSIEYFIKNTIIELILDKTILKSTNNKKKNKQQFTNYSQESLLKRNEILLSLLKINNSDKDELVLFNSVEYYNQQLFISFQKKSIQINLKISLNSANYQFEFDNYQYYQNTIKSKEWMNENTINNILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR59</accession>
    <name>A0A8J4PR59_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Dolichyl-phosphate beta-glucosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.117</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_006937</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a di-trans,poly-cis-dolichyl phosphate + UDP-alpha-D-glucose = a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate + UDP</text>
            <dbReference type="Rhea" id="RHEA:15401"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19502"/>
            <dbReference type="ChEBI" id="CHEBI:57525"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.117"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:15402"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.117" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000342">
        <property type="protein sequence ID" value="KAF2071752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR59"/>
    <dbReference type="OrthoDB" id="3784at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004581">
        <property type="term" value="F:dolichyl-phosphate beta-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04188">
        <property type="entry name" value="DPG_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000068">
        <property type="entry name" value="ALG5, dolichyl-phosphate beta-glucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035518">
        <property type="entry name" value="DPG_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859:SF91">
        <property type="entry name" value="DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859">
        <property type="entry name" value="GLYCOSYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="15">
        <location>
            <begin position="74"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00070518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2071752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="324" mass="37120" checksum="6F7EDFC27975F0B7" modified="2022-05-25" version="1">MTYTVETSSFLTSTPVLLIGVLLLSFLIWGIYLILIENEGVLWLPEENVYIDPKEGQVHQFPSITNDKSEVYLSVIVPAYNEQERLPKMMDETVKFLKDKQKKNINFTFEIIVIDDGSKDKTSQIVNSYIEKESTDIIRLLKLKQNRGKGGAVKRGVLCSRGKYCLFVDADGATEFSDFDRVENTMHKIEKKGLGIVCGSRAHLVDSDLVAKRSFLRNILMYGFHLFVEILCVKGIKDTQCGFKLFTRDSAKRIFPSLHIERWAFDVELLYIAQKLGDIPITEVAVNWTEVEGSKLDPFSSSIQMAKDILRIRVRYLFGIWRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRB0</accession>
    <name>A0A8J4PRB0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000358">
        <property type="protein sequence ID" value="KAF2071601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRB0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052050">
        <property type="entry name" value="SecEffector_AnkRepeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586:SF3">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="574"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="783" mass="92190" checksum="A7C90F4BDC40C8AA" modified="2022-05-25" version="1">MIINNHSKNVIITELYHQIFNNIFLRNNIFNQMKKIQRRGASNASKGYDDIVDLSWIIRNQHWGLLGLKLKRDDYLLLNNSDCIFQILDFNLFVSVFSKFQDYFIPQYPSLLHVAANYNNFAALKYLVELGYVGDRYQGECFSAAWRYKNYEMVRYLLELFKENIHLHVFLNVKCAIDSGDLEMLKYICATLYPLDLQLVNNPVRVQSFHHLAVNGGNIEIYKLVNKYYPLELTLPIFLASIKNFELFKYFMQEILDKNNLKSTATNQLEQQQIFTGEWLMEISNNIVIFRAYQTFFYLFENKLLDQALCNSIKNQLLIESFKQGNLEMYNILNNSNNNNQELKSLSISSIKNGLVSLDKVKEYFKMGLKITRECLNKSIYQRKSWEIFNFLWENFQDKDTFIQQFFGYNSFESHYWWWNDLTHDDSYFRFLKLLLESSLSLTKNNKNNNNNGNNSFHDQMGVVNGLNRSATFGSLEIFKLLYSVLDKSNFHDMTDFYHHAASNSQIDIIKFLLQEKAPHNNRVLCSSSCIEIVDLLIDSGHSLNSNVVENGFLPNLDVLKYIIEKIEMYNNNNNNNNNNNNNNNNNNNNNNNNNGNSNNGYNENIDNNVQNISYDSQIRLSISYSLVQHFKYLLDHSKTSYTNSQEILVNIGASGNLEMLKYYFNKYPKDKESYKDCFDGAVYSGHLQIVKFLIETLDPEFNYFFDTSNLKDLVQNRYTCMLEYLKKELTRLRPISNLWKIYNIDFINLLKDSDTYFNVYFDKYIHQLTDFTLKNKISNLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRC7</accession>
    <name>A0A8J4PRC7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_007797</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000436">
        <property type="protein sequence ID" value="KAF2070879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRC7"/>
    <dbReference type="OrthoDB" id="30833at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01831">
        <property type="entry name" value="Endoglucanase_E_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040794">
        <property type="entry name" value="CE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037461">
        <property type="entry name" value="CtCE2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17996">
        <property type="entry name" value="CE2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="482"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="5">
        <location>
            <begin position="5"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="4">
        <location>
            <begin position="234"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate esterase 2 N-terminal" evidence="3">
        <location>
            <begin position="522"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="SGNH hydrolase-type esterase" evidence="2">
        <location>
            <begin position="641"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2070879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="843" mass="93743" checksum="B7B91EF0067C9D62" modified="2022-05-25" version="1" fragment="single">STWISYNTTIEVPFNFGIVDKRFSISIYGAADRLMNLAGLTFDQLKNQSYNSIIKVENNAKDLIISDVEFINATLADDSIQENILIFGHIYNYDTLSGLKLIVKIFDKQGSLVKIYPYQELIQHSGLHIHIVKTFSLLEYTIHVEKNESNLIKMDSYNITLPKPLPFKPVYCTGFPPDIEVDPQPESTPSPTPPVVIPCPNDCGAPKGNGYCNNGECNCIYPYSGIDCSSILLNTTITPNTTDPSVTINDQKRFESILAVVGIRELDPQSTQVNSYELVSGNWLFVSSSESNDIYKQYEYVYQLPSTNITSIIQIFDKSHNITFGSQIINMQPSTVKFTFNITSYPFSSSLNSLQLVLMASFKSQESLGSTCSVTDFIEDQGSSEYLRIQIQDRSLYGRFIKYGLVEGRETAITNTLLQDYKQSIRKPDETQAYIGLNIPYYKNSVQLDPDFSVLIEHESASDRDNSICTQASKNGLTAAQIAGIVIGGVVFIVIVALALIFIISKKVSGLFIPPSNEKINYVGRVDKSVSSQYGLAWPGIQISVLVSGTTRFSTVFASTGQVYYNVYIDNVMFVMNVSSPLPQPYSFSESLILDPTQTYNITLIKRTEAMTGISTFYGILVDDNSTLISYPKTSSRSIEFIGDSITCGYGILGEAPCPFQAAQEDISLTYGGIISRELEAQLYVESWSGKGVVKNFGSITIPSNQTLPELYPLTNPTEYTDYWDFNDFLPDAVVINLGTNDYDGTAPFPSKKLFEHTYIDFINFIKSKYINNPLFFLVCGPMITDPCCQYVQNVAHAVDAIYIDMQGILEPNDYGCVGHPNVEGHAKMASIALPIIQKNMGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRD2</accession>
    <name>A0A8J4PRD2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Palmitoyltransferase</fullName>
            <ecNumber evidence="8">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_005835</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="8"/>
    <dbReference type="EMBL" id="AJWJ01000243">
        <property type="protein sequence ID" value="KAF2072858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRD2"/>
    <dbReference type="OrthoDB" id="6781668at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161:SF85">
        <property type="entry name" value="PALMITOYLTRANSFERASE HIP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="427"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="478"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="509"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="606"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="655"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="221"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="255"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="321"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="354"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="10">
        <location>
            <begin position="563"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="699"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="708"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2072858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="781" mass="88655" checksum="15BE5A07AA5CBE8A" modified="2022-05-25" version="1">MSSHGHSHGGSHGHSHGGSAHGHSHGGSSHGHSHGGGAHGHSHGNSNNNSEVGGSGYSYTSTRHYGDDDDNYAPPVPMTQQAKLEHTYQPLATNVDIPYLQILTPNQLKTAPQYVNQYLMLLDRWANQCIRKSDIAYTLPVPYSQVLVLLEIAKQGNLEEFKDYIETIKKEYIEKQSRDMILQAEQDESMTEEEKEDLFTNSATLVNKLSTKTIINFVDLEGCGSIHYAIMKKQKYMVQYLIDNGVDLNIANYDEGHTPLHWACIKADASFVYLLVENGADPFAVDKRGYNSVLHSAQYNEIHSIRYLLEKGLSVDTKDHQNHTGVHWASFQGHANMARFFITSGINPNLQDDTGRTALHWACLKGHLSVVTMLFTFKVELDIRDNDGRTPYEYASSKGFTDITKYLDSVKNSYTMFKGDENLYHRFWFLMGAFTILAPVLVLCALPLILAIPVIGVSGYFLKNYLQLNYWVPERNNWYHPSILYSSIIIWYTVYILKISHNTTMESGIVPHMLINVLIWAFFYYFLRLTKDDPGSICKHQSPADASKMFMESLAQGKSLPLICPTCHINRPVRSKHCPTCGGCYARFDHHCVWIKNCVALSNQPLFISVLFSYVAIVVCGFFITMDYYNFDPDHITFSEDWFGWFQYNFTNHTFIFVFMIYGVAMSFWIAKLAISQVMTIVFNKTTYEQIQEIRQFEKNHGHSHSGGGDHGHSHGDDGGHSHGGSSDSGNKYTSDPSFSNGSQNIDFNQYNHGPKNNIKEFLFYNQKYYYQTENEHGVHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRX2</accession>
    <name>A0A8J4PRX2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_006812</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="EMBL" id="AJWJ01000330">
        <property type="protein sequence ID" value="KAF2071885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRX2"/>
    <dbReference type="OrthoDB" id="5977743at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF473">
        <property type="entry name" value="PALMITOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="80"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="204"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="269"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="159"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="316"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="316"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2071885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="400" mass="46197" checksum="5B527920B797FAD8" modified="2022-05-25" version="1">MDLILSLIVGYVIFAVFILYTLILGQSDYHRDGVIGWCHYFLTAGLQIHCLKCFSKICPRKVRSSFGSCYHYFMYKPNRILQGLYLSLVLVGFYLFHKDAFPFLQAPYLEEFHKVGAIFMVSFTLITFIIASVSDPGIITTDNVDQYNVYDYDRYLYIKKHCETCNFTKPARSKHCRICDKCISRFDHHCPWINNCVGEKNLKYFLLFVLSTAILCLYGAFLCGSIMYSFVKVKDLFNLGFHDNGRWVPITTSMVVQFIAFETRSVLPLGILCIVISVFLFYFFAYHLYLVVKNTTTNETFKWSDIKDQIKLQKLNNSNNDNSKSTQEDSSSNNNSNSKSKQQKRNQNSKKKDALFPSAAIDKYTLTLPLPDSFKDLKNIYNRGVINNIIEVILPLSQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT22</accession>
    <name>A0A8J4PT22_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_006667</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="EMBL" id="AJWJ01000318">
        <property type="protein sequence ID" value="KAF2072004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT22"/>
    <dbReference type="OrthoDB" id="331948at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="27"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="126"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="327"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="356"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2072004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="396" mass="45514" checksum="390D8BE986D712AA" modified="2022-05-25" version="1">MDKSKNKGCSQRCKIKYNFFDKMSKRLTWLSGPLFVGAATGLITLVIYSYFVYIIPNVSDTRPIYFFYFNIDLILDILLSSFITFNIYFNYYKVISTDPGSPSFPTISGEDSEIENRNLNQSLQIKWDFCKKCQKPKPPRTHHCNVCERCVLKMDHHCPWVSGCVGFYNYRYFFLFLFYLWCGCAYLLIHGLPLFFSQDLYTMEYYEFERILILLSTVTSAMVVIVVGCFGGFHAYLIGTGQTTIENIIRSNRKPNYSLGSIQKNFDIVLGKGRFWFSGLLPTSKPPPGNGCYFKLLNSLQHQQYQQYQQQQQQTIAPLSTSIDIFNSDDDQHYDSDEMNNSNDDEESLVGLYPINSNSNNNNNNNYSAMSSVNNNSNSGNNDDNNNGNNGVYYTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTH3</accession>
    <name>A0A8J4PTH3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005541</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000223">
        <property type="protein sequence ID" value="KAF2073152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTH3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="433"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="239"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="306"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="326"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="477"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="488"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="508"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="556" mass="62910" checksum="1B70631208FDE8BD" modified="2022-05-25" version="1">MDAIMQDSLGDSFLQPPPLLEEKDDKTLIEEFSYSNSSIVHSSIETDKQDQQQQIADQDKSSHESFQDSLDGKVNDSTSNNTLGEQQQHHVEHQEHVETHEHHEHVENQEHQEHAEHLQEEDFIIQSLDQNMDDSFVESTNPNNTSMELPSSFECSNIENKQEKEGEEKGLMGESLVNDDISKTMDQQEEFKSSTDNQEVTTTTTIVDTTTETEGVAVPAETTTDTTTVNTTTTETEVLEVPTRTATETTTTETTTAETTGTTTTTTTEVKDDLDLDLEKLERNQKEEKEREVKEKDKETTPSPPLSTSTQQSTQQKQTKKPTIRTNFSKTFKSSTTPTTTTTTTTTSSISYTSPFRITQSTFFDLNSIKNKNELPNPLPSVKKIHDHKSNTHKNVYYLVSFASKSIKEKFWLSQDQVAKQIRDSYHKSLNTFGNLADSEEDDDDDDDDDDNQDSENEQEEEQEEEDEEIEEEFTKPVKKTNKKRKSSSSSKISSSSTKSKAAGKKTSKAETVEKKTSKAPKRVKKQKLEVESEPTPIKTQSRKGRVIKTIHSIIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTS2</accession>
    <name>A0A8J4PTS2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009371</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000691">
        <property type="protein sequence ID" value="KAF2069311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTS2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="341" mass="39249" checksum="869A10896235985C" modified="2022-05-25" version="1">MVKYDQWITPSFNQHWKTIEGENDDDGDKQNPLIFDFDKGFIPSSVTSISIRHQNDLFNWGQGEYPGDDWIPDSVTMLSVPKLPFHLVMVPKKIKYLQLSRAEYTYKIEADMLLPTTEYLWIKGPGTLNTHLIPNTIKEVVFSSCNLNGIRMKPTFTSPIHIKDPINYHGDIVSKLKHDVNVQSITLSHGSIILPPTFTSLIITPNKGFNNSLAALHPLPPHLKHLSLPYTYQQPIYQQQHIPHTLTHLELCVVIDKRNNRFTNDWLCRKGTRGDKRRFKRISITRELVLPESFKNHIHNVTLPSTLESLSINNPDYSNQLNNKNLPLSLTSFKCNSSVYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTX1</accession>
    <name>A0A8J4PTX1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Methenyltetrahydrofolate cyclohydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_009275</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(6R)-5,10-methenyltetrahydrofolate + H2O = (6R)-10-formyltetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="3.5.4.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000669">
        <property type="protein sequence ID" value="KAF2069401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTX1"/>
    <dbReference type="OrthoDB" id="5126881at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000001">
        <property type="entry name" value="Bifunctional protein FolD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000094">
        <property type="entry name" value="Bifunctional protein FolD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020867">
        <property type="entry name" value="THF_DH/CycHdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008058">
        <property type="entry name" value="PRK10792.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010783">
        <property type="entry name" value="PRK14186.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF5">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00767">
        <property type="entry name" value="THF_DHG_CYH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="10" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic" evidence="13">
        <location>
            <begin position="7"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding" evidence="14">
        <location>
            <begin position="143"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2069401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="288" mass="31347" checksum="80819B71A700BDF7" modified="2022-05-25" version="1">MEFGNLIDGKQISASIRLSVKEDVERLVSQGHRAPCLAVILVGERVDSQTYVRNKKKTAADLGIQSIDKVLPESATQQQVIDIVRSFNNDKDIDGILVQLPLPSHINEEAVLNEIDIAKDVDGFHPINIGKLSMRGRKPEFEPCTPKGCIELIDRSGISIQGKNAVVLGRSNIVGLPVAMMLMNRDATVTICHSKTKDLKEKCREADILVVAIGQTKLVKKDWIKPGAVVIDVGMNTDENGKLCGDVDFNECKQVAGHITPVPGGVGPMTIAMLMKNTLTSAQKKFCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTX8</accession>
    <name>A0A8J4PTX8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000197">
        <property type="protein sequence ID" value="KAF2073555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTX8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="641" mass="70837" checksum="2A5A71EBBDA01318" modified="2022-05-25" version="1">MEAIHIALQSHTIRDIITENVLEGVETVGATLKYFIRNVSSLERVSTRVELIVYQRHFDQYKDEMIKLGYQKLLKKVTFEDGFNSAIPVGFIPRCLSLNFGSTFDQSIEKGVIPEAVEELIFSENYSKKFEVGVIPNGVTSIRIGNLFVNGYEHPVEAGTFPSSLKKLYIKSIVGAAKGSLDMLEDLDVMTFGDYIPTVSKLRIVVPDNTTVNWVPTTVKELCLIAANYDSDDDLCPTIAPLHEGLESLRFQRNFREPFIHPLPSTLKVLDLRDTRINQQFSMNYLPEGLETLITSNSYKGPLLPGQLPKNLVTLKFGMDFANDGFPLAQGALPANLKSLKLGTDFDCELTAENLPRNLIELALDGGRRVLTPGILPDTITNLTVCASESIPAGAIPNSVKSLTFSRHHRADILAGSIPDSVEELYIEDGSVLSCSILPGTIPASVKKFIWNTRVYLSGCTADIPSGCHFECHLTTTGSYYSAPPADFITHITYLFDTPIPQGFFPKTLKNITLSHYHHPLPVGIFPEGLTKLDMRTYYKHPVAPGVIPSTVTTLAIPFNESYLNLGTKTMEIYITSANTGQVHPACENMFVDGSIPSSVKNLIIEGYPTSAASYSGPEKTIIYHMDYNQNKVDYFDVIGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU06</accession>
    <name>A0A8J4PU06_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Translocon-associated protein subunit gamma</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Signal sequence receptor subunit gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_005116</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAP-gamma family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000196">
        <property type="protein sequence ID" value="KAF2073567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU06"/>
    <dbReference type="OrthoDB" id="10059529at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009779">
        <property type="entry name" value="SSR3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13399:SF2">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13399">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN TRAP , GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07074">
        <property type="entry name" value="TRAP-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2073567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="173" mass="19584" checksum="F1DB823901E902AD" modified="2022-05-25" version="1">MVELDDEFSAFRNENIVSNEQRVVYFINSFIAALVPVYLYHAIFSMSFDSYMIVYGSVTLFAAIVLTFAYNNVYRVKRLKLSATREHMLIPKTKQTNGLDRKKVLAAKRESQAVVTSHEATALSIMYNNAVFLVSMLAFSFIIFKNVPLVYNYIVSVSLAAGFTTFLSTSSKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PV00</accession>
    <name>A0A8J4PV00_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_004634</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="AJWJ01000167">
        <property type="protein sequence ID" value="KAF2074065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PV00"/>
    <dbReference type="OrthoDB" id="346907at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14009">
        <property type="entry name" value="STKc_ATG1_ULK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048941">
        <property type="entry name" value="ATG1-like_MIT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21127">
        <property type="entry name" value="ATG1-like_MIT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="9"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="299"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="374"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="299"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="310"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="320"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="374"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="400"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2074065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="628" mass="70927" checksum="57795FD2AA4B19BB" modified="2022-05-25" version="1">MNVQYLNDYIIQGRIGSGSFAMVYRAVSKLTGEVFAIKEVNVHRLTEKNTKLIENLNYEIRILKLASHPNIVTLYDVIEPPQSDEYMYLVMECCEGGDFSKYIRKHKRLTEEKALYFMRQLSSGLRFLRQKDIIHRDLKPQNLLLSDSSENPILKIGDFGFAKFIDVQSLSETFCGSPLYMAPEILNHKSYTVKADLWSVGVILYEMLVGQPPFNSSSMKDLILQLDKKTVRLPSSLAISKGCQDLLFSLLQSNEMERISWEDFFSHPWLIGQSPAVSYPIQTTTNSIPFNIKGINNNNNNSNNNSNNNLPGYSTSAPTNNININSNNNIQNNNNYSTSPLDNGNLPFAFNNSASNKPIVLSPGSSFTERNLNKSKSFTENNQNNNIRYPFKDDKGKMGSSNGKNSNSNSNSNSSNSNSNTTVVIGTTTTTTTTTNPTTTNALLDFERDCVILDDQELFSTDRVLKRATVIAELGDLRQYEAAECLPLYLKALNMLKSRIPNTSNNNQASSLSEKFKSTFKEYQKKAFQIYSTLNSDQDWLNPQFSANKLIYETALDYGRKAAVEEMYTNYSKSLQLYIDGLLLIEYLLTISSDKDDQDTLKNYISAFNGRISIVNRRYLNSTTNTKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PV46</accession>
    <name>A0A8J4PV46_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_004605</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000166">
        <property type="protein sequence ID" value="KAF2074076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PV46"/>
    <dbReference type="OrthoDB" id="2162994at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051140">
        <property type="entry name" value="GATA_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658:SF128">
        <property type="entry name" value="GATA ZINC FINGER DOMAIN-CONTAINING PROTEIN 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="6">
        <location>
            <begin position="375"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="240"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="240"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="262"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2074076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="458" mass="50860" checksum="8C52906C21EA24E8" modified="2022-05-25" version="1">MNHQFVPSSSPSFCMSNFGIYSSSPAQSSMYNTPHSPRSPSSSFLLSPNIGYFESSPQEHHFSSPPSSMSTFSNGNRNNNCIEFKNNININNNNNLCSPPNSTNTTPRISKLDLSNLSSFNSNNSNNNNNNNNHHFTHEVHASSNSHLVSPRTLTSISDSLSSLRCMVDNQNPNVSNCLNNNNSNTTITSTTKPITNSNANGNPIPIPLLELKTNSPRSCSSNTNSPRSLYFVPESVSDVSPLQTPLSSPRTHSPRAMIMKNSSNGNANNNSGLTHSHDGRMEEHWKKMEYYVNDLSHFVYETLKSKDFSNILELKEKVDEVVNNAKEIEIMNNISKSLPPQTRARKKRSTKAEKLHKDLSIAGGVKRAYVTTPKSKGTYCNFCGTMETPEWRKGPGGHKTLCNACGLHYAKNIKKEESLKEQQSQYQEKEKLSPQTATMMSLANLVNFQQKNGDDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PV62</accession>
    <name>A0A8J4PV62_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase 2A activator</fullName>
            <ecNumber evidence="8">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Phosphotyrosyl phosphatase activator</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_004591</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for PP2A-like phosphatases modulating their activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a direct target of the PPIase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the PTPA-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="8"/>
    <dbReference type="EMBL" id="AJWJ01000165">
        <property type="protein sequence ID" value="KAF2074106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PV62"/>
    <dbReference type="OrthoDB" id="16120at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008160">
        <property type="term" value="F:protein tyrosine phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04087">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1150:FF:000002">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004327">
        <property type="entry name" value="Phstyr_phstse_ac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043170">
        <property type="entry name" value="PTPA_C_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037218">
        <property type="entry name" value="PTPA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012:SF0">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03095">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016325">
        <property type="entry name" value="Phstyr_phstse_ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140984">
        <property type="entry name" value="PTPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0697">Rotamase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2074106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="328" mass="38121" checksum="17611A8F4C8BC688" modified="2022-05-25" version="1">MANYVPSATSIVSAQHVEIEPIDINNHVFVKPIKIITDQTELNKFLCTKQCKHFIDFIQRLGDKIVNKPNSTETKISNLTENIIKMIKELELWIDSIPPLAQPMRYGNKAYRTFYQKLVDNIEQLHQQILPQQFHKAIIELTPYFLDSMGNQTRLDYGTGHELHFVVWMYCLERIGAFDDEDEVAMALKLFPAYLELVRKLQRVYGLEPAGTHGVWSLDDYQFIPFIWGGAQLAGNTLNLKPSSILIEKTVDQYYQDYMYFACIKYINTVKKGPFHEHSRDLFNISGSSSWEKIQHGMMKKFFDDVMTKVPIIQHFLFGSIVHFTLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVT4</accession>
    <name>A0A8J4PVT4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tetratricopeptide-like helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004201</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000144">
        <property type="protein sequence ID" value="KAF2074496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVT4"/>
    <dbReference type="OrthoDB" id="435413at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="90"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="797" mass="91578" checksum="F9040FC7389C55DD" modified="2022-05-25" version="1">MKIYVHFEKEPEFTLICEPLKKDLKTIKDLKSYFIEQYKASHGNPHEFSINSIDIKDQKNKTLLETTLITKVFSNLDDAFIFKNSNVDKPTTTTTTTPTPTATTTTPTPTTTTTTTTTQRTPPTSATPTTKQPPLTIQTLGPDGKVQTPSGKFKVEGCDARLKTIIESLLDRAEPLFLKKSYRTALEIYDQILKMHDEEKYSLLKSAEIYFQAKKYTRAEALIAKGIEAHPKEYQFHLLKARLKSENKDFFEACKSFKNVLNLVPKSNPVYNNLKAEYGTALYDTRVNDYAQQGCQLLNEVMGEDENNMYVMMGFAHVLIDRGQYEDAFNSLLRLASAAFPAQKEVTRFKEYGWKNVDTRPFKKEREWVQEKLTDVVMAKGLDMVYKAFEPQPIIQKPQIYGFVASMIKDNGAVNEATELLRKAFQLDKKTPNYILTLCHNLEVINKYPEAVEHVMTYLQVNRAKGIRQFKIDNGVVYDLVKPYLKNPIEDSYSFKLPMPEQGPTKVAEDLLVVNEKDHCDNEFYKEDYNKEELDYLSLWFTLTKILYTLGILEPLPALIKLLEPAKKGRDLHLTTVRNEQAYFSSVSMLMSYRSLPLPNHRPIYVAGDSHSLSTAWTPVTINGEPRLFHPLLTTGLKMWHLRPESRFFPKANFYNVTTTAPRGSEIVFLFGEIDCREGFVVSVDKCIYETIEEGMRVTIDIYIKALKDLISKYQYKIYIHPVVPVLDATRSMVKTFNKIFKEKILAQGNTFVWLDFFDQLLSPEGGFNLTYELDATHMNPSYVSLIESEINKNYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW47</accession>
    <name>A0A8J4PW47_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004481</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000159">
        <property type="protein sequence ID" value="KAF2074195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW47"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="107" mass="12344" checksum="A6A4D9209FE1E276" modified="2022-05-25" version="1">MQRIINYSQFKNNLLLNTNSSSVLSKNIFLLRNRDYSTIYRKQDGTTVRSPFPNQPHSDPKPTIPPKEISPYPPKDPNPNPTPFPNNPKKPKPTDFPIEDELYPQPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWJ4</accession>
    <name>A0A8J4PWJ4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003715</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000120">
        <property type="protein sequence ID" value="KAF2074976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWJ4"/>
    <dbReference type="OrthoDB" id="24295at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052697">
        <property type="entry name" value="FNIP_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32031:SF47">
        <property type="entry name" value="B BOX-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32031">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="977" mass="109448" checksum="79418A6B27AB02A3" modified="2022-05-25" version="1">MQCEHRHIINSIKFVDKNFLYYVPIGFFTSNIKKIKFPNDYNRDFKVGVFVEGLEYLYLGKSYNQTLEKGLLPSSLKHFYLGGKIKSDFPLSSINYFKTIEKDVLPDNLQHLSISGRLADDFTFPQNLKSLHLLKVSNLKLDPGYLPNTITDLHFGHSSLAAALKFGDIPDSCLKLNFGFFGAQLSHGVLPQNLLDLHMGTCIGSLLKEGVVPNTLTNLVLPNNNVPLKVGCIPMSIKKLKMGFSYNSPITGVIPSSVEKLYLSPSFKQPLQVGDIPASVTKLKVKSVLNLNQKTLPSNLKSLTFNPYIQTGLQPNDLPHGLVKLKNFNYHQDLEKVIPDSVKDLSIILKTCLKPGFIPTNVERLRIQCNKANLFVAKDVIPTSVKYLEFKCEQRESIQKLYIPNNIPIIKGTLVLSALEKILEPNEIPPCITKLYYHSSQPLLANIVPKTLKSLIIGDEYDGQTLLKDSLPIGLESLLICNNSIKFIGELDFSIPIGFFTSNIKKIKFPNDYNKEFKVGIFVEGLEYLYLGKSYNQFLEKGLLPCSLKHFFLGAKIKDQSFTQITSIINENVIEKDVLPDNLQHLSISGRLADDFTFPQNLKSLHLLKVSNLKLDPGYLPNTITDLHLGYSTILERLDIGSIPNGCLKLNFGYHCYEPLIGLLPLGIVDLHLGVSFGCNVLENFIPNTVTNLVLPNNNTPLQPGCIPMGIKKLRLGYSYNSPITGVIPSSVEKLYLSTTFKQQLQVGDIPASVTKLKVESVLNFGQNILPSNLKSLTLPLYVQITFQPNDLPLGLVKLKHLNYFQQEKIIPKSVRNLSIILSTNTLKPGFIPTNVEKLKIQSMKKANLFVGKNVIPTSVKYLEFELSQHGSIQKLQIPNNIPIVKGTLYLAGLKKPLEPNEIPTCITKLYYLDAQPLLANVFPKTLKTLYIASHLYIHPLLKDSLPIGLEGLHRFDSNICKYITIIKCIKKTIIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWT3</accession>
    <name>A0A8J4PWT3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003450</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000108">
        <property type="protein sequence ID" value="KAF2075232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWT3"/>
    <dbReference type="OrthoDB" id="19974at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="118"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="162"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="299" mass="33882" checksum="34C981992B20D438" modified="2022-05-25" version="1">MRKFYWNKRIMHRYLAYAVMIPLTMTAVTGFLYKILLDVIGVQKEKIKFLLKLHQMSILFPNSAVFYTTLLTTIVTALVVFGYSMIYNPLWSRSSRSGRNNRFVPQSMREYHHRYSSVIFIILLFMTLSGGVYRFLRAVVGIEKEDIHWLLELHHMGFLPNWLSVIWVTFAFLCIFSLLVSGGRILPLINSLFRPSKYQVLASGTSTPPTPSANDISIIVASGGSSSGGSGNIDTLGNHDINEIDVEEETYPINSAYNNNSNNNNHSILNTSPTDIDYQHYNSSSSSLSPRDNDLKDSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWW1</accession>
    <name>A0A8J4PWW1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007682</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000423">
        <property type="protein sequence ID" value="KAF2071006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWW1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="133" mass="15702" checksum="E017CAE56276E389" modified="2022-05-25" version="1">MDYITSDIESLIDKITVSRISNEDKEMLMTKIYHHHHEELYLKYIESELIQMEKDYLYQLKMKETERKLKHFTTSLNAKGFILNKLSDQVDSLHVSMEKERESSSTKAHKVEPISNDMLVNILLNNSKKVVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWX2</accession>
    <name>A0A8J4PWX2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005251</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000204">
        <property type="protein sequence ID" value="KAF2073434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWX2"/>
    <dbReference type="OrthoDB" id="1057137at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047574">
        <property type="entry name" value="AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039683">
        <property type="entry name" value="Lsm12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019181">
        <property type="entry name" value="LSM12_ABD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13542">
        <property type="entry name" value="LSM12 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09793">
        <property type="entry name" value="AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00995">
        <property type="entry name" value="AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52001">
        <property type="entry name" value="AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AD" evidence="2">
        <location>
            <begin position="106"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS52001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="205" mass="22546" checksum="804E61B7FD31EDAE" modified="2022-05-25" version="1">MEDKYTWVVGLKVKIKTTSNEQFEGEVFNYDQNTTCLTLLSDESTAAVFTHKRTVRILLESSISEVTCIGLPQSKSGGNSLNSSSGSGSSINSSKGGNDSYDLSLPPININSILKRQEDSMRKAHLNASRIGVGVTAEAQEVFNSLIKTLPCTWSGDNIIVLNEVKISSPYNVENCTGPEITRERVKKVLEAERKKMLREKQKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX21</accession>
    <name>A0A8J4PX21_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000106">
        <property type="protein sequence ID" value="KAF2075291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX21"/>
    <dbReference type="OrthoDB" id="19196at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="416"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="426"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="458" mass="52671" checksum="2439445EA2F2850C" modified="2022-05-25" version="1">MQAIRNKFQKTSRQTKEGLGVHKEEQSVIKPETHSLRNESNIIKRDYHTLSLQSDKWIAALQDTQTKWDILASAITNFGDGLYTDNSNNRRIAEVQRAMQLLYFPLIKTAKLGIYDNASRISNYDIDQLNKIQHELDGSRLRADHHNHRLMKYKAKPNANPNKLRKREITYEKYQSEYDSLFRRYQELIRDLEFKSEDNLIHSRQLLLDLQKFFNDGYSLIHPAQKEINSLPLNERLISRMADNNIHLKQTSFQQAKQQMNSTSLLNKNMNTLNLSPDSDSDINNNNMNLNKNSGTISPMILNPSTSSIQKTTESTTVIEQKPATPKMEGSSSVQKTTTESTTVIEQKPIVQDKPMFKQDMKQVEDSDATTVTYQTIPAQIVEPEEMELKDTEPKSTIVAPKIIREYVYDPNVVERPVESQSVESKSTESKSVEKVVETTEFKEGQVVEKTVEKTIIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXN2</accession>
    <name>A0A8J4PXN2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zinc-ribbon 15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002756</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000079">
        <property type="protein sequence ID" value="KAF2075953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXN2"/>
    <dbReference type="OrthoDB" id="5545479at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031493">
        <property type="entry name" value="Zinc_ribbon_15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28139">
        <property type="entry name" value="UPF0768 PROTEIN YBL029C-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28139:SF1">
        <property type="entry name" value="UPF0768 PROTEIN YBL029C-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17032">
        <property type="entry name" value="Zn_ribbon_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zinc-ribbon 15" evidence="2">
        <location>
            <begin position="24"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="190" mass="22407" checksum="34E61B49C86792E8" modified="2022-05-25" version="1">MWIPIIIPFGTTNKSKVLDNEIRTCVNCHNTGVQLVRNDAWAHLFFLPFCKVKSGQPYLHCPSCGATIPYVANAQTPKYADPNLQFQPKGPGMPIPQQQQQQQQHYPMYQDNPEYQYQQQHDMGYENPHMASQYPNDNPEYQQQPQQQQQPYYVDQPEEGKEKKKDKKKKKEKEKDSTKKHRHHFFSKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYM0</accession>
    <name>A0A8J4PYM0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Multiple inositol polyphosphate phosphatase 1</fullName>
            <ecNumber evidence="4">3.1.3.62</ecNumber>
            <ecNumber evidence="3">3.1.3.80</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">2,3-bisphosphoglycerate 3-phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">CYY_001156</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(2R)-2,3-bisphosphoglycerate + H2O = (2R)-2-phosphoglycerate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27381"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58248"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="3.1.3.80"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:27382"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>1D-myo-inositol 1,2,4,5,6-pentakisphosphate + H2O = 1D-myo-inositol 1,2,5,6-tetrakisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77115"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57798"/>
            <dbReference type="ChEBI" id="CHEBI:195535"/>
            <dbReference type="EC" id="3.1.3.62"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:77116"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1D-myo-inositol 1,2,5,6-tetrakisphosphate + H2O = 1D-myo-inositol 1,2,6-trisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77119"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:195535"/>
            <dbReference type="ChEBI" id="CHEBI:195537"/>
            <dbReference type="EC" id="3.1.3.62"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:77120"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>1D-myo-inositol hexakisphosphate + H2O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16989"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57798"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="EC" id="3.1.3.62"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:16990"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histidine acid phosphatase family. MINPP1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.62" evidence="4"/>
    <dbReference type="EC" id="3.1.3.80" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000026">
        <property type="protein sequence ID" value="KAF2077538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYM0"/>
    <dbReference type="OrthoDB" id="6509975at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034417">
        <property type="term" value="F:bisphosphoglycerate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052745">
        <property type="term" value="F:inositol phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033379">
        <property type="entry name" value="Acid_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016274">
        <property type="entry name" value="Histidine_acid_Pase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963:SF8">
        <property type="entry name" value="MULTIPLE INOSITOL POLYPHOSPHATE PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963">
        <property type="entry name" value="MULTIPLE INOSITOL POLYPHOSPHATE PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000894">
        <property type="entry name" value="Acid_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00616">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035211968" description="Multiple inositol polyphosphate phosphatase 1" evidence="17">
        <location>
            <begin position="19"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="53"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="245"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00043671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00043691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00043832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000894-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF2077538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="428" mass="49403" checksum="D766774DA6C5DB45" modified="2022-05-25" version="1">MKLVFLCFILFLVDFSTCCRDITNEKGFHLSTKTRYQLPQIENTYNEYLKKNCKLIHINYIGRHGTRNPSVTETLSLSSLGQSFKNQEGNIYKEYKWILDFIAPKNESAGILLPVGKYELFEIGKRIKSRFKNFFNPFSSEFYQVFSTKVPRAFESASFLINGLFNGSFVEDNGGLVFTNDQENLLRFFDSCSKFLGYLESGENVIEMNLWKSYSYPPIAKRISLLIGLNETMSIKVLDKLFSACVNEASINNNYNDWCSLFTKEDIVNWEYAQDLKQFWTRSYGNQLNYKMASLLFQEIIKDLENSIKGGQDSFSARIRLAHAETLLPIISILGLYKDTYNLYANLTSSQINSRKFKTSNISPYAANLALYLFKCDGTIKISVEHNEVPVLIPGCKGKLCDFFLFKNLFNNALSFVWDAYCYETYKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYR0</accession>
    <name>A0A8J4PYR0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glucose-6-phosphate 1-dehydrogenase</fullName>
            <ecNumber evidence="7">1.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_002826</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15841"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="1.1.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glucose-6-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.49" evidence="7"/>
    <dbReference type="EMBL" id="AJWJ01000082">
        <property type="protein sequence ID" value="KAF2075890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYR0"/>
    <dbReference type="OrthoDB" id="60984at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00408"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000018">
        <property type="entry name" value="Glucose-6-phosphate 1-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00966">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019796">
        <property type="entry name" value="G6P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022675">
        <property type="entry name" value="G6P_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00871">
        <property type="entry name" value="zwf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429:SF0">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02781">
        <property type="entry name" value="G6PD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000110">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00079">
        <property type="entry name" value="G6PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00069">
        <property type="entry name" value="G6P_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3 7" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="4 7" id="KW-0521">NADP</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase NAD-binding" evidence="8">
        <location>
            <begin position="17"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase C-terminal" evidence="9">
        <location>
            <begin position="196"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2075890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="497" mass="56938" checksum="8D6F103A3688CFC2" modified="2022-05-25" version="1">MSVSPNANDYRTPLTVVILGASGDLAKKKTYPALFSLYLRSLLPENTIIYGYARSHIELDDFKKRIGSYLKGSEEKKKAFLDLCHYHSGLYDDKSAYDEFDKLIRKEEAKIQSPTSKYNRLFYMAIPPSIFIEVSKGIHGSLIENNGWSRVIVEKPFGRDLESSRVLVSELGRLFKEKDLFRIDHYLGKEMVQNLMVLRFSNAVFEPLWSKSHISNITITFKEDIGTEGRGGYFDQFGIIRDVMQNHLLQVLSLVAMEPPLSLNADDITNEKVRLLRCIQPIKLDEVVLGQYIGTQDGKQEGYLDDKGVPNDSKTPTYAAAVFHVNNPRWRGTPFILKCGKALDERKTEIRIQFKRPDNFLFQDEDISRNELVMRIQPGEAVYLKLLTKKPGLDNSIEQTELDLSYRNRFENLDLPDAYERLILDSIKGDHNLFVRDDELDVAWQIFTPLLKQIESLGCIPEKYVFGSRGPKSADELSKKFGYVRSTGYNWPGSSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYY3</accession>
    <name>A0A8J4PYY3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUS-like FMN-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_002481</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000068">
        <property type="protein sequence ID" value="KAF2076240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYY3"/>
    <dbReference type="OrthoDB" id="10262250at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017150">
        <property type="term" value="F:tRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052582">
        <property type="entry name" value="tRNA-DUS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45936">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(20) SYNTHASE [NAD(P)+]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45936:SF1">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(20) SYNTHASE [NAD(P)+]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="7">
        <location>
            <begin position="34"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="366"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="398"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2076240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="416" mass="46105" checksum="B1A1B180F9CE3820" modified="2022-05-25" version="1">MSDIVTNSCTSNASPLRSLSKTDVVMDYYNKKIMAPMVRIGTLPMRLLALENGCDIAYSEELIDFKVKNTTRVVNEKLNTVDFISKDKSLTYRTCDADVRNVLQLGTASSVTALEAANVVVNDVCAIDINMGCPKFFSVQGGMGSALLSQPEKIKDILSTLIRNLPIPVTCKIRLLATDNETIDLLKIIENTGVKAIAVHARMIPERPRDPAHWDRLENILSNASFSVPIIVNGDIFEHGDIASSLAKTKANSVMIARGAIRNASIFSQPVATNVHKLIQRYIQIAYENDNHAVNTKYNITNILNENLMTQTPEFKSIQSAKDYPAFCNIFNISSQYEKFVQSQKVEAKNYISVSVQNEDLVAKDNENSCSNKNSNNNNNDENSSNNNNNKHELPGNSILNEDSDNNPQALKKQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ22</accession>
    <name>A0A8J4PZ22_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_006758</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the G-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000326">
        <property type="protein sequence ID" value="KAF2071921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ22"/>
    <dbReference type="OrthoDB" id="5817230at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141124">
        <property type="term" value="P:intracellular signaling cassette"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002307">
        <property type="entry name" value="Guanine nucleotide-binding protein G(k) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000007">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF324">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-6 SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="42"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="148"/>
            <end position="149"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="173"/>
            <end position="179"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="198"/>
            <end position="202"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="267"/>
            <end position="270"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2071921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="348" mass="40966" checksum="4CDAFBA8B304E4F3" modified="2022-05-25" version="1">MNCRTSPKSQEEQVSIEIDKEINRNKRRIRKDVQILLLGAGESGKSTIFKQLKILQDNGAWKPEELMDYRNTIYVNITTQIHVLINACEKFNLPLLPENIERSSRIKDRNHSNAEWNSQIADDTMHLWNDPSIKSAFEKRDKEFQLNDSAEYFFTNLTRISEPDYVPNPQDALRTRIMTKGIVEAEVIFDQMNMKIIDVGGQRAQRRKWIHCFDGVSAVIFVAGLSEYDQYLREDNRVNRMDDSLELFQEICNSRWFINTSMILFLNKRDIFIEKLKRVPFNIYDPRYQGQNNYEETSVYIKNKFHSVRENKTKDIYTHFTIAVDSQNINFVFSAVKEIILKQVLEGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZG8</accession>
    <name>A0A8J4PZG8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_002830</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000083">
        <property type="protein sequence ID" value="KAF2075844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057709">
        <property type="entry name" value="DUF7949"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25820">
        <property type="entry name" value="DUF7949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035322142" description="EGF-like domain-containing protein" evidence="3">
        <location>
            <begin position="26"/>
            <end position="1352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1309"/>
            <end position="1332"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="6">
        <location>
            <begin position="637"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="5">
        <location>
            <begin position="774"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7949" evidence="7">
        <location>
            <begin position="1016"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="4">
        <location>
            <begin position="1074"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="987"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="988"/>
            <end position="1008"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2075844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1352" mass="149536" checksum="2F9D023AC844E085" modified="2022-05-25" version="1">MGEIKYIGVFLFLLSLLLGCERVSTFQIIDYTPPYLRNVYSLGLTPQCIASYYLVLEDPNGIEYGGGANIVADGTQLLERNTTHALYKMVFSFGSSSGSVPIQLRNSLGDSQNINLNYTCKQPPTNPNIQLIGSPYFPFAKGDMPVFRFKTTGLDPLYRPISFAFRDSTLALLGETTGYSSNDFIATVNILPNGFNWDSLKADTPVYIYYNQFSSTPSYGTNTLPALLPTGTNQINSKNQQISSIQRDGYFLSIQTCLNNDDLVFQTGYLTAGGSSMIPTLMQGNYLSPTFFQYFYSVTSSSYNQIYFNPSAVYSSFAYPYDINFINLAISSTPSLSRVGDLILRYFTTENLGSRVHWYSLRVQANGTSTYLPYPFGYQLSYSTTYSKQFEFLFSPYTNKVVRARGDSPSYILTSTTPDTLPPFIKSATVSLLPQSTNRLFRLNIGDDISGFYEISNMGNFTAKTSGSNLDGVYDFIVTQYDYIRLSNNSLLCDFARNCRQLNLFEPFNLNGDYINQDILKLSDINSISFDKNDIDISNSHVSNKLFIVTKPGTSKALPVVMVMVKEGLSIDTTTMIFNGQWDETEGCYVVPFNVHQNYITGVYPYYLYIDNKPVPYSHLKLFFGETASLRIKSNNGDIMGPIINQFSVSTVNQGSTKTLTLNIGIVDKVNGFKKGIISLITAYDLVEHNKTFTSDDKQGGDIYQSTYAINYSFTLNCRSQNINISYIELEDQQGHVSIFNFFQQPPNVINPIMSVPKPALTLVCSGPAENITPTLTAFSFTPTLISPFSVSSRTVVFNFQSSDSSGINLASVPFIYLHDYSMQIKGFPSTLIDSNETSANYTAVVEVPYGFGDERGLSVSVYGIVDVYSNFRGYSNSIMPFPNNISVSPSGSNDQIIFSTSSLSVRGGSLTIYGKNFNLPNTDVQISFSNSSIYQNVSPTTIEPNILIVNVNALNEPFIFVTIRQLSTQYVYSNSYKVVLESIETDSSSSSGGMSSSSNENTDSSGSQPTPPPNPCKNNCGGDNGICTLKGCQCYYPWTGAECSSQIIIVVPTINTTKPDTSIDSKGDNGVFNSVISLVALNELGSKGELIYQYKFQSWIVTPNENSVYKQFSPVSYKYETNITHPLYETVSYINVFIDFFNQSEPVAISFANEILTMNPSSLKYSVNITTYSFSSSLNTLQLVMATSIESKDTQCSAQETGNTVDNSEFIKLQVDNHSLYGRFIKRGIIDGRVRAVTNNLLLNSDLYLSNSTTSKSSAFIGINIPQFSRLVQIDPDFSVLIDNKAANADSLNSICSEKKSKLSNAQLAGIIVGSVAFGTAIIACIIYCIVKKRKDVKFMNKLKLKFIASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0F2</accession>
    <name>A0A8J4Q0F2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sidoreflexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_000034</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the sideroflexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000001">
        <property type="protein sequence ID" value="KAF2078663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0F2"/>
    <dbReference type="OrthoDB" id="6608471at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015075">
        <property type="term" value="F:monoatomic ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990542">
        <property type="term" value="P:mitochondrial transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004686">
        <property type="entry name" value="Mtc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00798">
        <property type="entry name" value="mtc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153">
        <property type="entry name" value="SIDEROFLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153:SF6">
        <property type="entry name" value="SIDEROFLEXIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03820">
        <property type="entry name" value="SFXNs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="104"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2078663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="338" mass="36992" checksum="5E9E45626EEE7B7A" modified="2022-05-25" version="1">MEASVMKPKTTASGLPFFDGVSKKFDDKTFWGRYQNFRDVTDPSTLFCTEQQLEQSKQLLDQFKKGLVDPKTHSDELWTAKKNVDAIVHPDTGKSIFLPFRMSAFLPINVIICAGLILPNASMGTTIFWQWINQSYNIALNHANRNASNTMSNRQVLEAYGSAVGISCSLAVGLGWGVDKLKIASPALLSGLKMMVPFTAVTSAGIANVLIMRKNEMIDGIDVKDKDGNIVGKSQVAGKSAVMKVAVSRAATSFPALLLPNILMKGFERLSVVQKYPWTKLPLNLAIIGAIFNTSLPAAIALFPQESTISAKDLEPQFQNLKDKNGNPITEFIYNKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0H6</accession>
    <name>A0A8J4Q0H6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Succinate dehydrogenase assembly factor 4, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_002910</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SDHAF4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000086">
        <property type="protein sequence ID" value="KAF2075777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0H6"/>
    <dbReference type="OrthoDB" id="201362at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012875">
        <property type="entry name" value="SDHF4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28524">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28524:SF3">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07896">
        <property type="entry name" value="DUF1674"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="57"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="85"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="98" mass="11392" checksum="63282D4A1264D688" modified="2022-05-25" version="1">MQSNIMIKRVMSQFRFRSNNIRFYSTTNTIPNNINNPKGSTTISQENQQIIRELEEEQEEDFDFSPYVNPVTGEVGGPKGPEPTRYNDWERAGRVSDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0I6</accession>
    <name>A0A8J4Q0I6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Arylamine N-acetyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arylamine N-acetyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000022">
        <property type="protein sequence ID" value="KAF2077693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0I6"/>
    <dbReference type="OrthoDB" id="17550at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2140.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001447">
        <property type="entry name" value="Arylamine_N-AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053710">
        <property type="entry name" value="Arylamine_NAT_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786:SF0">
        <property type="entry name" value="ARYLAMINE N-ACETYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786">
        <property type="entry name" value="N-HYDROXYARYLAMINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00797">
        <property type="entry name" value="Acetyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="284" mass="32890" checksum="1A8A0867406E2B8D" modified="2022-05-25" version="1">MYVNIFKNLFLEHVKFPQNFRINNLKDLSKIMKAFQSTTPYNNLNILDGSWGIETFDKITKKVLIEKTGGFCYQLNSVLNHFMKVNGMNVKLVKAFKSDKNLHENLRFHFLNILNYNGKVCIVDVAAGSASPLQPVVIDCQSEKDIVIGLNGNQCRSIKHTHGTFLYQTKSPNDREWKTIYHFNLRPVIGLDEFHHAQFYGGVKHSYFNQRPLVNILTADKSLKVSSIFTLTDSSFTKTKKENQKTKKKIENNQMFNSYLQNQFNYGKSFEIKQSYHDSLNKPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0W4</accession>
    <name>A0A8J4Q0W4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Histidine kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_002941</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000087">
        <property type="protein sequence ID" value="KAF2075759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16922">
        <property type="entry name" value="HATPase_EvgS-ArcB-TorS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339:SF1">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13188">
        <property type="entry name" value="PAS_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0807">Transducer</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="315"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="543"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="8">
        <location>
            <begin position="1347"/>
            <end position="1474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="414"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="688"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="839"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1075"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1159"/>
            <end position="1189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1303"/>
            <end position="1327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="416"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="426"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="458"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="692"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1075"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1097"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1176"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="1396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2075759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1524" mass="170937" checksum="E7F87E1FA72AF371" modified="2022-05-25" version="1">MKDLRNSNILFPTSIPPGNNIILSNNNGQLNTSTTINGVNSNNNNNNNNSNNNNNGINNLDHSLSSLSSLSTSTNASYNSTNRININNDENDNNNSNHFSKYHNQNSIFQNILKNLKEKLLPPRIFKPSIYRRECLLLLEVVIYFFAYLFLLIFFREFYPVFVVGMVSMIIIYIMSLKLKRYDIVSCSYIIVQSILNFSFFFYFLVYHNNNSVHSSTSTATSNLTVNSVLNSPISENLISSTTATTSVSNEDFWTSSDFYKNNHLFLLNFLMSLLSITVFFPKFLICIVFLVFLNFINAIVHVLAHYSYSTPSFIIFQNLLISLAITLLLICYCYISSIDIKEIEYKEKTFRNIFNNSSEAIVIHKKGIIIDANSTFEKIFQIKLDSILYPVPSGIWEFLPEIESFFEKKKKLDQENQMTHTSRPTNNNINNTQQQQRQQRKYRSTRINNLTRKDSSNMNPNNQTNQNENNIDNTFIFDTIGINTLGEEFSVQVKLGNESNKNVDFDVLTIIDVSARKKLINADKALRKAEEINQAKINFLTTVSHEVRTPINGILASAEILEKSFLDMTQRDFLNCIKDSADYLLDLITDILDFSKIEAGKMELEKIEFNLVTMLEESLNIVYRTAKERGLELVLFIDKDVPMILIGDPYRVKQILLNFLSNAIKFTPKGQVIVRVKLLSSQRKSNYKTKSTSSSSSSSSSSSSSSSSPKISSSIVNNLSTSSAANSNTSNNNINPVQLYEYNICFEVEDSGIGIKEDMINHLFTAFIQLGDSPRKYLGTGLGLSISKRLCNLMRGEINVKSQYGVGSTFSFSSVLQSPINQAPITVGELGKKLSINPSIQPSSPRNPPSPSSSSSSPKTFKCFIYDDNIQVSKSICAFIKLLGIDANYLVSEQSLATTLENEILNEPEENTLYIIIASQLSDSNQSIIRDKMCFNPFIFTFLLTDSLVADNNIKDSLYHGVLKKPCNFPNIVETLYKTQMYPIPRDYYQLLTNKSLEDYDFYFKIHQQSVKPNNTPKYKFISATIDSSSTSSGNGSSFLSNGSNKSKLFERKNSSGNYSDHIAITRGLVQVNRSPRTNISSRSGSHSNFYDDESPSNSTLSTPTSSPFSNLLQNNVLNNISNSLLSQINNNILKEQQQQIQQQQQLLLNDKNIGGENNTIVMEKPPLNLIKSSSHKDKDKDKNNNNININININNNNNINNNNNESNTTTPIINSPQILMEKDDHNSEHIIIPIDELKLSSPETPPLINKSPTRKRGLSIDIVVPDLKLDAILLASSKQCASPPLMVQSLSPPLSSSISKASVSLPPLPQQPSLTTSNSSILSSSPPLENDKIFGSLIGKIDKFKILLVDDNSVNIKVFSKFLKDGGFTIDIAWNGVQAVDSYKKNYYPIIFMDCHMDSMDGYTATKIIREFEGEVISTFPEFKRSTIIALTALDVGGNGSGKDRCFNVGMNDFIQKPVRNSKVMINLIERYMKGYSNEDIDFSSQSILKYSLVPSPSLLSTLSSSSLSSWSTKFSPSSSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q181</accession>
    <name>A0A8J4Q181_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000732</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000015">
        <property type="protein sequence ID" value="KAF2077931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q181"/>
    <dbReference type="OrthoDB" id="10249920at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047631">
        <property type="term" value="F:ADP-ribose diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006753">
        <property type="term" value="P:nucleoside phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019693">
        <property type="term" value="P:ribose phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18888">
        <property type="entry name" value="NUDIX_ADPRase_Nudt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839:SF1">
        <property type="entry name" value="ADP-SUGAR PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839">
        <property type="entry name" value="UDP/ADP-SUGAR PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="52"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="203" mass="23254" checksum="F4EDDC9354F5D2F9" modified="2022-05-25" version="1">MSESLISHDVSKQIKINSLCRAKWLELNEIVYYDHQGRVRTWESVSRTTKVSEVDSSDIIATVNKDGKKYIILTLQFRPPVNNFVLEFPAGLIDPNESIENASLRELKEETGYQADQVLLTSPPLPLESGAADCTSKIVIVNITEDKKEKQELEDTESIEVVLLPFDNLFDHLEDERKKRNCLVNIQLYSFALGLSFRDKFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1G8</accession>
    <name>A0A8J4Q1G8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">G8 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_000508</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the comF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000009">
        <property type="protein sequence ID" value="KAF2078218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2016282at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055401">
        <property type="entry name" value="CEMIP_beta-hel_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019316">
        <property type="entry name" value="G8_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052334">
        <property type="entry name" value="G8_domain-comF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47687:SF4">
        <property type="entry name" value="G8 DOMAIN-CONTAINING PROTEIN DDB_G0286311-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47687">
        <property type="entry name" value="G8 DOMAIN-CONTAINING PROTEIN DDB_G0288475-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24606">
        <property type="entry name" value="CEMIP_beta-hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10162">
        <property type="entry name" value="G8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01225">
        <property type="entry name" value="G8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51484">
        <property type="entry name" value="G8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="G8" evidence="6">
        <location>
            <begin position="579"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="77"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="83"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2078218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1415" mass="153764" checksum="ADB7E1AA568B3B0E" modified="2022-05-25" version="1">MRLVNNRLLSFVHSYDIELSGTNFVLDASALETQITYTVDNSLSKPIISLTLTRGSGVLVSDLVSVSADKKTISLGDYKKEPTTSSDPTTSTSATGSATSSGGSTPSSQETTNPEINSSNRLNGLFSFSFLVSILTFGLYYSLGSSSGNGSRSKSGLIILSALVLFIGCGIALNLDDVALKVEIKAPASFVFDSLTINLGKGSSSVSSLRSQVLVINGCNQKSHSFSMSNVYASNLNVCTQTDVSITNLEVPSSSKLNITSSAAVTLGFLNQYAGSVDFISKSPSISGDCQTSTSGQQTTGTCNGGGSSNLIVSGSAITVDLVATCPTTSTWRPTPASGTAPTSPAISTSYPARTFVWNQPTDWKIVGHYGFEGTTLSTVTTGTSNGVSIANSLKFTIGYADWGPTHYSLALRKGFPSLKTGVAYTFDFSFLLGEVAGSYNTISNMTLYLIDRVDLSGTSQYFETPTQYKYKYSLTSSYTSTNAWVTKSIPLTVTQPIGDVTMILQMNRTGQTGNVVRNYYFKDFRVTIPASTVPTPTLITKESQYITIPKPDSTVVDVQTPSTCPYLATDLVHWHDPATWGGTVPSPSSVITLPANKKVLISPCSISTDIYQKIIIPQGSSLIFADSDITMNIKDILVQGQLIIGTKTCRYNANIELVFYGAKTTSDTIAQYFGSKGIAGTATSFISIQGKQYHNTWTRLAATVWPGDKIIYLLDAVNWEVGQQVVITTSIMEDELSNQNEVMTIAAIQGKTVEFTTPFNFYHYGGKEYQSEVGLLSRKIVIRGDPTSSDADSFGGHILTMGEGQFSGIRLHKMGQKNMKARYPVHFHLANTLLNSYITDCAVHQSYYRCYTIHGTNNLLLSRNVGFDINGHCYYLEDGVEENNTISYNLGAYVHTIGPAATGYDQSGQMFYENPLLTQPADSSAGCFYFTNAYNKIIGNSASGGWAGFSFPNLAKPIGNFQTSTVVPQSRSTLVFEGNTAHSSGYQWEFGGSLYVGGNLYVNTSDSGRLAYSSGRNSRETVDSANNNVWMRFNNTKMYLSNRGIDMWGERVEVVNLESHDSNRPATLFGEAWFDQGYVNGQSGNILSNSQKYDRQGFQFYDTYVKTILSNIKFANFIAKTSPASDEEDNRVIISMTHSDVFKPQGMSVTKNITFENVAAAQKIGHNVVDTGSSRYFNYIDSDGSFSGRGVPTLIGSHVNWWNFDSSCTYDSTWKTWVCNKGTKEIVNIEVLINNLIPSPNSNNNKIGNTTLFGSSITDRRSCVVTQNEGITGVSNMGWYMFLTGGSPTFQVINLKQIPYNQYIFLAIPYPANTQFTIYSYYKWNTSYNIKFTLAASAAAVRSGNGRTYYFDGSFLYVKLVDHTLNPTGESFARGGAQVFNYNWGYSYNITATCAGASGGFCPVTADNLPSSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1J3</accession>
    <name>A0A8J4Q1J3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptide transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_000582</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000011">
        <property type="protein sequence ID" value="KAF2078111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1J3"/>
    <dbReference type="OrthoDB" id="16350at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000649">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="210"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="327"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="411"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="441"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="477"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="506"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="538"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="564"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="570"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="606"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="621"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2078111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="641" mass="71627" checksum="DD9B47A705ABE975" modified="2022-05-25" version="1">MGMDEDGILAKPRLIAGDATPISLEKTSVSLEKQFDQKLNSSLEIPNGSHPTVYDEDGNEIKYGGDEKHFPPSIKFIMGNEIIERYSFYGLKSILNNYMIHYMGYTDTTAASIYHGFNAGAYFTTLLGAYLADGKLGKYKTILYFSIIYCIGGVFLAISAIPGVTGPEPGNRSPWGLILGLLLIALGTGGIKPVISAFCGDQFGPHQAKLLANIFQVFYWCVNLGSAFSTIITPIVRTNLGYWVAFLIPSCLLVVSISVFVFGNKTYIKKKPTGSVLLTSARIIKTGISEKIKSKKPDYDDQLYQGHWLDRSKGKFDPITVDQMKNVLKVLLIFLPLPIFWALYDQGGSIWIDQANLMDRKIGSVTIDSEILTALNPILIMIFVPIFEYCLYRPLAKKGVNFSPLRRMSVGMFLTFVSFMVSMFVQFAIDAKPPGTVNIGLQIPQIVIMAFAEILISITGLEFAYSQSPPQMKSLIMSMWLITVSIGNLFDTFVIALIKLTFWQSYLFFSCCMFGFFVLFVFISLKYKPIEINYNDPITHPNSNHTAGDDDYQNGEGKDQQIYDQIQQQQDHDDYNKTRNIPDNESDHSSSSRGSGHSEGYQSIAEKNKDQEELQKEIDELSSNSDESTSPIFKSVPLNKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q257</accession>
    <name>A0A8J4Q257_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PIPK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001350</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000031">
        <property type="protein sequence ID" value="KAF2077347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q257"/>
    <dbReference type="OrthoDB" id="20783at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00139">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF142">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL PHOSPHATE KINASE DDB_G0267588-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="3">
        <location>
            <begin position="247"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="28"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="522"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="119"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="643" mass="72994" checksum="2316B333D1EF03F8" modified="2022-05-25" version="1">MSSAEVINTTQADTVVVIDKKDDSLESIIIETPNSTPNVDGATTKNIKKEEQQQQQEQQQEQQEQEQQQQEQEEEQDQQTKPKRKEDSIGDVVDNEKEVPNNEISSDDVPEELMKSNKSEINTQQVLLNPNTTDDTKPTTTTTTTTTTTTTTTATNDHPLSPPIDPEGLEPIIDKNLESLQGDDIRASSSSLSLDSPNRGRLNTSQRLKLKQGKEKRLSHRPKSIKFEHKKNVLGDIIYKGHPSWALMLNIQTGIRNAVGKSMGTEGIAKTPQQYHTLRKPSEFRTKEEFYVSPKTLRFDSAGSSQTNAHSTGPFKFKDYCPTAFRYLRYLFGIDTADFMVSLCNTLKNGENALRELPTPGKSGSLFFFSHDMKFIIKTIPKDEAKLLRSILPSYLEHIESNPNSLLPRFFGLFRVKPHGGRQVRFVVMGNLFPTKKKIHERYDLKGSVVGREASEDEKKSESVTFKDIDFRIRKQKIHLGPNKKGPFLDQIKKDCNLLQSLNIMDYSLLIGIHYNQRDPPSPTLLRHVNGGGDDSDYESSPSIPSRSKRTTREEVDLMDTQEVYISAFQQDEGGVRALNEENLPMEEHYFLGIIDILMLYSLRKKIEHTYKTLKFGAKQEISSVSPLEYSQRFQEFLASIIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2X1</accession>
    <name>A0A8J4Q2X1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001651</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000041">
        <property type="protein sequence ID" value="KAF2077019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2X1"/>
    <dbReference type="OrthoDB" id="843225at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31964">
        <property type="entry name" value="ADENINE NUCLEOTIDE ALPHA HYDROLASES-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31964:SF113">
        <property type="entry name" value="USPA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="2"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="160" mass="17918" checksum="E6E4847C1DD9EB44" modified="2022-05-25" version="1">MKVLLAIDGSEGSTQAIQECSKLLKPNRDSITVITVINSESLAHEDYISNQADEYQNISEKILDRCLFHLHNVGFKDIQRKVLVGEVRDEIMKYMNQDTFEMVIVGSRGLGAFKKLILGSVSEYLVQHAPIPVYVVKKVSSQDDHPPSSTKNIRSLVPSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q308</accession>
    <name>A0A8J4Q308_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ornithine cyclodeaminase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000349</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ornithine cyclodeaminase/mu-crystallin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000006">
        <property type="protein sequence ID" value="KAF2078365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q308"/>
    <dbReference type="OrthoDB" id="41492at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1780.10">
        <property type="entry name" value="ornithine cyclodeaminase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003462">
        <property type="entry name" value="ODC_Mu_crystall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023401">
        <property type="entry name" value="ODC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812:SF19">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02423">
        <property type="entry name" value="OCD_Mu_crystall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001439">
        <property type="entry name" value="CryM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="371" mass="39660" checksum="8B2509A3A52907C5" modified="2022-05-25" version="1">MLIIKESDVYTLLKLQESIDVNENVFRLHSDSKVDCPSRTLIKVVDNGNNNDIGKSGEAGSVDNSSKPTTTTATPTTNLCYFKPAYVEGNGVGLKIVSVFPDNIKKGLPTIPATIVLLDENTGFTKAIVGATYLTGVRTAAGSAVVAKRFASEESKVLTIFGTGLQAQLHLQMILLVRKSIEKVVVVSKSLDRALAFIKESQEKLKDSKEITLSKDIEFVAELDGNKAVQDSDIVVTATAATPTPLFDGAVLVEKAKLKKIVVLSVGSSTPANRETDTFLFKNSNVIVDDINSCSKSGELSIPETEEKGFNSSTHIRGELGDVLTGKKPLDASNNLILFKSAGTAIQDIATAALVYQRAKELGIGIDVNLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3G0</accession>
    <name>A0A8J4Q3G0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000663</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000013">
        <property type="protein sequence ID" value="KAF2078025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3G0"/>
    <dbReference type="OrthoDB" id="444265at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050923">
        <property type="entry name" value="Cell_Proc_Reg/RNA_Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23308">
        <property type="entry name" value="NUCLEAR INHIBITOR OF PROTEIN PHOSPHATASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="236"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="197"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="316" mass="36885" checksum="67260958262B2976" modified="2022-05-25" version="1">MSNKIKYQNSDDEDDLAEIKRVQAARANKTKYTDEDEDEFDNFIPTSLPSSSSSKSKKDKKKKKHDKDDDDDEQRSTWQRELDSYSAPKEILDSIPIDEDNDPIKKYRAPTVAEQETEYRSRWRKRDLSPPRTYDPFTGKGEVKGRSYRDILIETQLAKEEKELELQMSKKLKEEKEKEQKKRKLEEYDNKYKTNHSGSSASSSSSNGKNRRWFYKVSKNQEIVEKDREIIAESPLTFGRDQGRNKVVLEHPSCSSVHAKITLERNGVRPILIDLNSTNQTFLNDKEIIPNKLNNLYEGDIIKFGASSREYIIYHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3M5</accession>
    <name>A0A8J4Q3M5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Fucosyltransferase</fullName>
            <ecNumber evidence="12">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_001198</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Golgi apparatus</location>
            <location evidence="12">Golgi stack membrane</location>
            <topology evidence="12">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the glycosyltransferase 10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="12"/>
    <dbReference type="EMBL" id="AJWJ01000027">
        <property type="protein sequence ID" value="KAF2077499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3M5"/>
    <dbReference type="OrthoDB" id="427096at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046920">
        <property type="term" value="F:alpha-(1-&gt;3)-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11660:FF:000002">
        <property type="entry name" value="Alpha-(1,3)-fucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11660">
        <property type="entry name" value="Glycosyl transferase family 10, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055270">
        <property type="entry name" value="Glyco_tran_10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001503">
        <property type="entry name" value="Glyco_trans_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038577">
        <property type="entry name" value="GT10-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929">
        <property type="entry name" value="ALPHA- 1,3 -FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929:SF237">
        <property type="entry name" value="FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00852">
        <property type="entry name" value="Glyco_transf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13385">
        <property type="entry name" value="Laminin_G_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase C-terminal" evidence="13">
        <location>
            <begin position="184"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2077499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="607" mass="69171" checksum="383059C4ECB4D639" modified="2022-05-25" version="1">MINIHCQKSSTVTAITKSQQLLFVFIVLSSIYIVHVQAVGILIWSGGTWCGYGSRTYTTPNYVGQNIIKPKCSVDCEYMTDKAYASAADAILFEAQPFAGFGYEFLKNQPNFPQKQVNQLYINFGYEHEVYFPVLAQERFHENMDVNNTFRKTDHVRITFACSWGSYDNGSIEEFRNPPVAPENKIQAIGFMSSNCNGGGAIYRTCYTKDLMNHIQVDALGSCMHNKDLDEEDKPHPLFSDLGDSLRIKRKVLSKYMFSLAFENNNMTDYVTEKVYTSLLSGSVPIYMGAPNIDDWVPKGSVIKTSDFKNPKHLADHLNYLLNNHTAYNEYFNWKKEPYPSGFVEKYNQCVFYSGDCRLCEHVSKTIKKMKSTQQAGHRSQFGEPIDTMVQKRALLLSKSSCLSVSQNSSIPKITDQMTIMFWINPTSSFSNDAKIITNNKKQFEIYISPIWKRSYIKFCIHQDNCFTGEKPIKNDEWKHVAVTVSPNPLIPNTEVVSLYLNGVLDISFITNSISSIDSSHDIKIGCQSQFVGQVDDFSIWNIALDQTSISRSMFKKYRGDEHGLNAYMTFNHHDSILDYSKHRSLVSSKDCKTIEIEDKKLDLNCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3U2</accession>
    <name>A0A8J4Q3U2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000008">
        <property type="protein sequence ID" value="KAF2078250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3U2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="12"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="115" mass="13508" checksum="9CBD97246CEC4336" modified="2022-05-25" version="1">MIHNNNNSKNNSSELNNDFIQLINAIKRLEEQQSNLNVQTKELKEFIPLARTKLKEISNRNDELEANLSRIEKENLQCKRDIDKLNLEINEELCTAKDYHQDDQCSIVLLNKNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4D6</accession>
    <name>A0A8J4Q4D6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004980</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000187">
        <property type="protein sequence ID" value="KAF2073721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4D6"/>
    <dbReference type="OrthoDB" id="19108at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="144" mass="16531" checksum="BF2A2857AB61EF25" modified="2022-05-25" version="1">MLSNLTRLCTRGSSTFVRQSVRCYSTDSYDLARKAETSNSISELDSVLKEVKDKQITDISVYDVLMRKYSKIQPSDGNCAEVFITADILKISDKFSQESLDIYSNVMKSHLLDDNDDLSGLDYITKDYQAYKESKTRRPWLNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPD0</accession>
    <name>A0A8J4UPD0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009633</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000762">
        <property type="protein sequence ID" value="KAF2069046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UPD0"/>
    <dbReference type="OrthoDB" id="18769at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="62"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="193"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="62"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="441" mass="49701" checksum="E16C3AFF771D4D75" modified="2022-05-25" version="1">MIFRKVTKFVNVNCTTTTIGSGSFSGGRRYLYINNNSSNSSGSSSSIVNKFYSTISNSNNSTDHISGDSSSNSNIPKDREINDASGGHVSAPSFNIPKDVEEFDNNKSNTSTKESGKATATTTTTTTLPGCWCLIGCKLKGKRIHVSKIKPIKYDTKRGDIGSDEYIGNCDIETSQSRKDMITELCKAKDQYVIEQKKELDEEEKLQQEKLQEQQEIKKIFSEDDDSSKSYPNLLEDDTSIGPIHVLYKNQWVITIVERMETDHKDNEPSFILKFKTPIRQKNDMTFSIESLDTMFSSIDKLLSFTKSVEIDKEFDQMYLTKSSSVPTKQETKTLLEQRPYMKEALIKLKASLLPSIGFEKCSIDVEYKKENPLGCKGGNTDLEFPDGVYQFCLEVDSNEHSIWLKSPEAVEKILYLYTIMLDTIVSMGNTLDTPIDTTYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPG0</accession>
    <name>A0A8J4UPG0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009486</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000721">
        <property type="protein sequence ID" value="KAF2069196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UPG0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="254" mass="29431" checksum="14E82F8F21FC7884" modified="2022-05-25" version="1">MEFYKAFDQFKLLHEICTCLFYPCYLLTLLYWIDKNLSKENPFMSEKENYISFTILFIPTFLVKIGCIVILGLKDNTTSHIHKILLSFYTGLIGVLMVLTLGILAYKKICHKFNSYKPMIHLFIISFTFMIYNLFYLFSTYTSQLPRIVHTVIYQALGPILLFLMLLLVYPIRNNLKQESKQDTSNATEMKKRIPPLKTETSINFGSETPRLHFEMGGEIGTPRGHFGIETPRNHAFSPETPREMEPLSASGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQQ7</accession>
    <name>A0A8J4UQQ7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">mitogen-activated protein kinase kinase</fullName>
            <ecNumber evidence="7">2.7.12.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_007671</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.2" evidence="7"/>
    <dbReference type="EMBL" id="AJWJ01000422">
        <property type="protein sequence ID" value="KAF2071011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQQ7"/>
    <dbReference type="OrthoDB" id="10252354at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06623">
        <property type="entry name" value="PKc_MAPKK_plant_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000716">
        <property type="entry name" value="Mitogen-activated protein kinase kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000432">
        <property type="entry name" value="mitogen-activated protein kinase kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013:SF32">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="124"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="49"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="332"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="54"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="71"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="332"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2071011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="454" mass="50630" checksum="4B0F337E90B00F66" modified="2022-05-25" version="1">MAFRGKRGNLRINVDNTPTIDKDANSFKLTNSGTFKEGDLAINKKGLLIKGESPKNSPVNERNRPKSTFSNFNPNNNQDNNNNNNNNNNNNNTIDNYTFNNNSNNINSQQPKLDSIISLDLKDLQVIKTLGLGAGGLVKLVYHSNSNTFFALKVITLDIQDTIRKHIILELKTLHKTLCPFIVSFYDAFYTDGSIYIALEFMQRGSITDILKQTKTIPEPILANIACQVLQGLTYLHRQLHLIHRDIKPSNILINDQGEAKIADFGVSGQLQHTLSKAVTWVGTVTYMSPERISGRSYAFDSDIWSLGLTILEMALGKFPYDPSLQLNFINNNNNNNNNNNNNNNNNNNNNNNNNNAENVENGMGFWVLLDSIVKGPVPLPSSADFSPEFCSFITDCLQKEPEDRPTASMLLNHPFLKKYRSVDNSSVVAWMNQNIPLSSPTATTPTSTTTPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UR56</accession>
    <name>A0A8J4UR56_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RhoGAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_007049</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000355">
        <property type="protein sequence ID" value="KAF2071641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UR56"/>
    <dbReference type="OrthoDB" id="437889at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042330">
        <property type="term" value="P:taxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016050">
        <property type="term" value="P:vesicle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07610">
        <property type="entry name" value="FCH_F-BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000060">
        <property type="entry name" value="Actin polymerization protein Bzz1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45876">
        <property type="entry name" value="FI04035P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45876:SF11">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN HOMOLOG 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-BAR" evidence="7">
        <location>
            <begin position="13"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="6">
        <location>
            <begin position="398"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="294"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="595"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="958"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1025"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="299"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="360"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="376"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="600"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="615"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="628"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="966"/>
            <end position="989"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2071641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1173" mass="131142" checksum="48D56A2E643402FC" modified="2022-05-25" version="1">MAIESNINNSLVAESFSDLWDGYDVVTKSSEKGVLLLKDVIRFFKKRIMSEDEYSKSLSKLVLKFEPQAPDGFVGARLKNTWDQIRLETLTHSNYHETICTNIQTHIIEPIEGLIVDLEQKLKAIHVDAEKVFMAYQESVAKLKKHKANYDRLCKDSFEITGVSKGETQKVQKRAIKAAQDVIKADKEYRGQINETNGAQKVFLSESIPKIMNDLQRLEMIRIHMVKSFFLRYFKSIEGMPQKFSTEHDSLVQLIQLINNEEEIQEFVRKVKPSHKYPKPFEYEPYLDKQVYPTPSPPVLSSSPTQTPSYLYPSSPSSTSSYNGNSSNSASVASTTSTISADCNNNNNTPTKKPGWNLKRFSTNLTAHKSSTNIKSNNGTPTSGGSSNSSNSSNLFTCNIEDLMNEQKKKYPNTEVPYLLIILKDKLKQLDAYKTQGIFRVPGNVIDINNLKKRFGEGNYEIAVNENVYTVASLLKSWLREIPDPLFAPTVYEQCLVATKKEEVQEIIDSLPLLNQKIISYIVTILQEAILPENVEVSKMNSDNLAMVFSPCFLRSPYTDPNILLGNIFKEKEFVKCLIDTLVPLSVNENIQEKAVVSPPSSPLSRLPPPPASPTNSGIKANSTSSVNNTLSPTTSASANSTSSLGTPNNITGKPTFKSDLLSPIAESPSADSGKRDLLPNPKIAAIKMESAITTPVKNPDGSISLQLMSPSEVKKRCDDSYLDNQEKVKELIDNIHTSINELYSDVTTIELTSYFAMQASLLIAKFTRSLEQFLTVDWKLTLADVIQNIDKNKFVSPPAIHFKIPSKLPKLLPVTLLDSDSTCDTLRDWLSTITLTINRVNEYLCYFGGVVMRIHSPETLESIRSLFSDIEMKPVDNPKFAALSAEQASLLIQKTLPLISPLNPFTQEELNINIKVLDTITPVDTPPFASPSNHSTYINKNAVDPLSGGTMGSLLTVPNKGSSGVNTPPSSNNINSSSNNTVSPYSSSPLQFSFDIPHGDDDSTLNSSGMDSDEDEIDLIEYNNKFKKLQVQESNQQIEAARHQQQQLHRSKTFEIIEYIKEQKKCLNQLDMGDPQAQTDMRMLAKFALSLKRDLDEFVNNYNESNDKVIEVELENDPVINKDNDSCMNLKIMTNHFMTRIIIILTTIHSYSSTNANLSLETYNLLSKYPIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UV55</accession>
    <name>A0A8J4UV55_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein mL43</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_009873</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL43 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000838">
        <property type="protein sequence ID" value="KAF2068803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UV55"/>
    <dbReference type="OrthoDB" id="88at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039927">
        <property type="entry name" value="Ribosomal_mL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007741">
        <property type="entry name" value="Ribosomal_mL43/mS25/NADH_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21396">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21396:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05047">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00916">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein/NADH dehydrogenase" evidence="7">
        <location>
            <begin position="18"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2068803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="127" mass="14743" checksum="F9961D20B074F8D0" modified="2022-05-25" version="1">MSRNGVWQLQKLIIRYCDLSGSSRDLRNVMNTTLKEFKTNNPQIEFEETLKRGQHPLAIATYKSGVTKTVPLRGRSEEKILKHIQNLRDTTGEKPRKFGNRYIQTTQSIQGLWNPFIEFSDSNTQTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVE0</accession>
    <name>A0A8J4UVE0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002226</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000059">
        <property type="protein sequence ID" value="KAF2076486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVE0"/>
    <dbReference type="OrthoDB" id="192262at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="19881" checksum="A5C8916286B53A4C" modified="2022-05-25" version="1">MVKFRFPTNKNAYDSPEQAASQTAKYADKLFPNLPNRNSSSFVKFSFSLLLDIVGFFIQIIPLSYLFYPAFSSYLLYRMYGSGLGVLVGFIEESIPGLGFVPTSLLCWANERYDLISLAGKYVPFINQLGGIKTVVNNIIKCLKMVVYAIIAFFVYTLYRLFFGGGGNSALGNQSSFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVV1</accession>
    <name>A0A8J4UVV1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Alkylglycerone-phosphate synthase</fullName>
            <shortName evidence="11">Alkyl-DHAP synthase</shortName>
            <ecNumber evidence="4 11">2.5.1.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_001759</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the exchange of an acyl for a long-chain alkyl group and the formation of the ether bond in the biosynthesis of ether phospholipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a long chain fatty alcohol + a 1-acylglycerone 3-phosphate = a 1-O-alkylglycerone 3-phosphate + a long-chain fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36171"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17135"/>
            <dbReference type="ChEBI" id="CHEBI:57534"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:73315"/>
            <dbReference type="EC" id="2.5.1.26"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Glycerolipid metabolism; ether lipid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.26" evidence="4 11"/>
    <dbReference type="EMBL" id="AJWJ01000044">
        <property type="protein sequence ID" value="KAF2076921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVV1"/>
    <dbReference type="OrthoDB" id="7786253at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008609">
        <property type="term" value="F:alkylglycerone-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.45.10:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.40">
        <property type="entry name" value="FAD-linked oxidase, cap domain/gating helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025650">
        <property type="entry name" value="Alkyl-DHAP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46568">
        <property type="entry name" value="ALKYLDIHYDROXYACETONEPHOSPHATE SYNTHASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46568:SF1">
        <property type="entry name" value="ALKYLDIHYDROXYACETONEPHOSPHATE SYNTHASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 11" id="KW-0274">FAD</keyword>
    <keyword evidence="5 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="13">
        <location>
            <begin position="148"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="519"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="248"/>
            <end position="254"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="261"/>
            <end position="264"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="312"/>
            <end position="318"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="458"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for enzyme activity" evidence="10">
        <location>
            <position position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR625650-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR625650-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR625650-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR625650-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2076921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="622" mass="69901" checksum="6E0D2D57CC558654" modified="2022-05-25" version="1">MQLKEETTTTTTNGSGNEKKDFPKEFVDSYKQIKWNGWGDTRKFLHTLSPSGQIAITTPKKSAEPLPGLKPFILKELAGGNGQSELEDTPSIEFDNIQIDPPKSNPEFLKELKAFFLSDQLKDDKFARITHTFGKSIRDLVRTRIGQIKYAPDLIVLPHSHEEVEKLVQTANKFNVVIIPYGGGSNIVGAVEPISNERFTVSVDMRRMNKILWVDRREMTACIQVGILGPDLERELAKSGVSMGHDPDSFEFSTLGGWLATCSSGHQSDKYGDIEDMAVSFKTVTPTGTLELRNGARAGAGINYKHIILGSEGTLGIITEAIMKVHPLPPVAEYYGFLFPSFAHAVSALEQIRSTDIIPTMIRIYDPDETRLSFASKPSKGAFTEFMSAAVKKYLHYIRSYDFNNVCLSIIGFEGSKKSVDFAKAHVFDILAKNSAFSLGTSPGKTWGEKRYDLPYIRDFVLDHNMWVDVAETTVSWANLIPLWKDAKAAFKEHFRAQGVPAWICAHISHTYSNGVCLYFIFVSKQNAEKEMTPYIQAKRLMTDIVMKYGGALSHHHGVGYEHVPWMNRYVSNGWITVYRKLKETLDPNNICNPRKLIPTLQEENHQTPYLFDVLNVKKAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVY7</accession>
    <name>A0A8J4UVY7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitotic checkpoint regulator, MAD2B-interacting-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001720</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000042">
        <property type="protein sequence ID" value="KAF2077011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVY7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018800">
        <property type="entry name" value="PRCC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13621">
        <property type="entry name" value="PROLINE-RICH PROTEIN PRCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13621:SF2">
        <property type="entry name" value="PROLINE-RICH PROTEIN PRCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="389"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="110"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="228"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="272"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="289"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="416"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="438" mass="49852" checksum="99086707E62712C4" modified="2022-05-25" version="1">MNALAGYGDSDDSDVSDSENSIVVQQPLVVNRPPPAIAPQPQPQPHKVAPTTTIISTASLLKRKKKDTDDKDEPGSVLSKSLFGQEEEQDKPAIPVADKAQQPEPKKQNTTTSSFTHSLFSMLPAPKSNTPTPTTENQTDKSKGESQIKQTDTDKSSDDNDNVDDDDEYEEDDDVETQDIDTPETIKIKQELEGIQKKISNSMIPGALRKTLLKKQVDLNDQLKKIKASTVVAKSSSSSTKTRVKKKKPIDQDSNNKQRTTTQQKEEEEESFFSFQPKVQQQQQPKLEEYDDEDEDEEEDTNANVSLPQQQEWTEEQINEYWNQYYQQQQYQQQQEQYQPIDQTIKNAHQYLRHYDMNNKGIVEISQNQLVKPSSTLDQAQLEGRYRPLDTGASRGVSGMQKNKHQITSLLSDFNTGRESKERQRAEERLQFASRNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UW76</accession>
    <name>A0A8J4UW76_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein HGH1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_009366</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HGH1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000690">
        <property type="protein sequence ID" value="KAF2069313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UW76"/>
    <dbReference type="OrthoDB" id="338814at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039717">
        <property type="entry name" value="Hgh1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007206">
        <property type="entry name" value="Protein_HGH1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007205">
        <property type="entry name" value="Protein_HGH1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13387">
        <property type="entry name" value="PROTEIN HGH1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13387:SF9">
        <property type="entry name" value="PROTEIN HGH1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04063">
        <property type="entry name" value="DUF383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04064">
        <property type="entry name" value="DUF384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein HGH1 N-terminal" evidence="3">
        <location>
            <begin position="93"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Protein HGH1 C-terminal" evidence="4">
        <location>
            <begin position="275"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2069313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="355" mass="40585" checksum="122841C3FABF8A72" modified="2022-05-25" version="1">MEQLAELVPFLADPKSEIKILALQHLSGVSDNQEARDVLKKTNIVTQLIKLMNDSNHVISRHSLTILINLCQDTDLLAEITKKNIVPRLVDGATDTKNKLSEIFAMLLSNVTHTKEGCLQLMQCGKELEAFFIMKLVQVLTMDSSADDYLTSTKNNWVVNIILNVTQIQEGRKIVLDPENSIFSSLLPLINHKNVIKRRGIVGIIRNCCFSEIHHDYLISEKIDIFTKILLPIRGSGKFDDDDMEGMNSILHNVNLPIDYLIEQDRECKRMLVESLVFLTGTRKLRVHMRKIKVYPVLRDFFKDETDDDIKESVEKVVELIIRDEEGEGQTFDGPQQKVKEIIEDEDAPFEVEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWH0</accession>
    <name>A0A8J4UWH0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009130</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000642">
        <property type="protein sequence ID" value="KAF2069553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWH0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053331">
        <property type="entry name" value="EGF-like_comC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24032:SF17">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24032">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035223447" evidence="2">
        <location>
            <begin position="21"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="560"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="562"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="632" mass="69739" checksum="8BFBD9FB19E051C0" modified="2022-05-25" version="1">MDKILFVCLIVCALVCSSSAFLNSNDYQKINILVGKINETFPLGTSQSNICSRTSNSLLIKCMTTFGGGGNESSIYQISISSTNPSSPSLNATDFQGFSILSQLRLSRVILSNQFLLDLNIYVPKIIDLTIDNSGIYDIPKGIKYETLEINYEQTFNDQYLSTISKIQNLFVTYGSGFNLYNDFTAPTEISLRNLRLKTYSFPDFSNVVTQSPIELTIYPSSNLDISNSTKIGNLQIKSIEFGSRNDVNLINVTFPKFIETYSAYPFDFYSNNIKWNLDRPLDLSGSKCKSLIFRDGAGLNVIGSDQYPISKASPMGMTVKLNQSGLKQANFSVISQTSSTYLNGNQITMDIPDMLFLNSSDIFARVVDGDGNKRNQLSLDVANNRFRGTIPNFFCNLWVDFSNASFIGQLPSCYTCFLNSSDIRALVKNNAFTNYMDIMPPSFYPPCTTIKINNFLVAKPKSYMPSSIINVFYIIGKDFGINSNIVAQVSGFGKNLTFSPLKYNEIYYIELSSNDANTLKAANQMIVTFVVPQISITLDISRVFNNVYDANALIITPFDAPSSTPEPSNTPSNTPSDTPSNTPSTTPSSPTTNSTTSTIGPEETEPNLGSMIQLPLLSLYLILTIAATILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWR8</accession>
    <name>A0A8J4UWR8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Poly [ADP-ribose] polymerase</fullName>
            <shortName evidence="7">PARP</shortName>
            <ecNumber evidence="7">2.4.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_000866</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.-" evidence="7"/>
    <dbReference type="EMBL" id="AJWJ01000018">
        <property type="protein sequence ID" value="KAF2077821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWR8"/>
    <dbReference type="OrthoDB" id="1729737at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.142.10">
        <property type="entry name" value="Poly(ADP-ribose) polymerase, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031273">
        <property type="entry name" value="PARP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004102">
        <property type="entry name" value="Poly(ADP-ribose)pol_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036616">
        <property type="entry name" value="Poly(ADP-ribose)pol_reg_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036930">
        <property type="entry name" value="WGR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46530">
        <property type="entry name" value="PROTEIN MONO-ADP-RIBOSYLTRANSFERASE PARP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46530:SF1">
        <property type="entry name" value="PROTEIN MONO-ADP-RIBOSYLTRANSFERASE PARP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02877">
        <property type="entry name" value="PARP_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47587">
        <property type="entry name" value="Domain of poly(ADP-ribose) polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142921">
        <property type="entry name" value="WGR domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51060">
        <property type="entry name" value="PARP_ALPHA_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BRCT" evidence="8">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="PARP alpha-helical" evidence="10">
        <location>
            <begin position="285"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="PARP catalytic" evidence="9">
        <location>
            <begin position="416"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2077821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="619" mass="70128" checksum="895FB86EA5CCB908" modified="2022-05-25" version="1">MEPNHLQSIKFLIDSRAKFTDITNFKLSGLVKQLGGSLSYTINKQVDYVITNDTSLPEYLKTNSKVLSQCSNTKFLNVAYFCQFNDKSTTIAPIENFVFDQKEFSVDETKKKTKNDDEAHQIRHEIKNNPRYIATNVYELKDRQPSFPDEYMVLIKRLLTCTYLGYNTNKFYYIELQQATDLNLTKHFRIYTEFGRTDQVDRSNAQHRYPDCYDDAINIFSSIYSQKLEDNSGYQIAEMTHTNIGSKKKYEFLKTGKFPQDESNSTGTAGSKYQIENQDFQNSYIEKSVASLISTIYQEASKCLLKNFSVKITNRGIETPLGALSMDQLNAASTVLNDISKEINSTNNNDTLVTLTSKFFQYVPSKLTVRNKSIKNNASVVISTNDNVIQYVELLDMMKDLTRVYLKNENNQESKNELDMQYSALNTEIHHLSSASSEYGMVINLLKKNPRDADALSDGRIKISNIYRLIKDSEEKQFKDDIGNVQYLFHGSKIQNFVGLLSKGILMPNVVVSKGGSRSDFGFLGTGIYFNTDIKSSLYYASASSGANGVRMILMSQVALGSVKEYSKINSTLTSPPTNFHSCKGVSVSSCPGSDFRHDEHVIYDPTQTKLSYLIEFTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWW9</accession>
    <name>A0A8J4UWW9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_008476</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000526">
        <property type="protein sequence ID" value="KAF2070200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWW9"/>
    <dbReference type="OrthoDB" id="10255964at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031982">
        <property type="term" value="C:vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042330">
        <property type="term" value="P:taxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016050">
        <property type="term" value="P:vesicle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000060">
        <property type="entry name" value="Actin polymerization protein Bzz1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000072">
        <property type="entry name" value="Unconventional Myosin IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036274">
        <property type="entry name" value="HR1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF7">
        <property type="entry name" value="NOSTRIN, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01887">
        <property type="entry name" value="SPECTRNALPHA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46585">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="F-BAR" evidence="11">
        <location>
            <begin position="1"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="10">
        <location>
            <begin position="434"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="397"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="403"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2070200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="493" mass="55320" checksum="BEE5DF02C447CCCE" modified="2022-05-25" version="1">MSYSSDLLDGFEILYKRTEGVLKCNQEMSNFFKKLSTLESDYSKALLKLVKSTGQKKLIANPFLDGSSKSSWNTTLTELEHVGNQHQVFANLTNDLSNLIDKMVKDKENVRKKLTGDGQKLTKDMKQQLDICTKSKLNYHKLVKEAEVAQTLLNKGQADPKMKQDKVSQLSVKATQAFEKATQADQEYRETLGVTNTKQDEYYHTDMPNLLKEFQAFEEDRIVQGKDIMHKLATYIKEIPPVLNHAAEDIEKGAEQIDKDQDIKQWVSENRTGVTVPSHIEYHPYEGEGQSSSNNSPNNFRVGTYKPPTTQIKEWGLTAKDQNLGANEKIAKLEQQYSEISVNIRSEVQARSGIEKLIQFYASDPKEQKKAEGELAEADRKIVSLKESQKLITQQLEELGKNPQLSNSTGDVQHNGGNAGVPGVQAAAGSGVQQKVVRVVGLYDYDASCDTELSFREGDILTVTEQDSSGWWFAELNGATGFVPQNYVEEMKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UX63</accession>
    <name>A0A8J4UX63_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008163</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000481">
        <property type="protein sequence ID" value="KAF2070515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UX63"/>
    <dbReference type="OrthoDB" id="17232at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="169" mass="19365" checksum="5896A11C42876902" modified="2022-05-25" version="1">MDQEMSISYDDYQSKIREAENKLVEISKLEIQENLLVILTKEHEIDSFLNKVSPILSNTELPLYVLSTVLNLILTGELGTFEDSRKSVCISGRVLIDKIKSFNVDQVSFHTFQILRGYFISEESESMNLNPTFTLEHIEPYGEAPSALYKWIDANFSILTVIYDEDADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UX71</accession>
    <name>A0A8J4UX71_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">General transcription factor IIH subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">TFIIH basal transcription factor complex subunit 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_008135</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the 7-subunit TFIIH core complex composed of XPB/repB, XPD/repD, gtf2h1, gtf2h2, gtf2h3, gtf2h4 and gtf2h5, which is active in NER. The core complex associates with the 3-subunit CDK-activating kinase (CAK) module composed of cycH/cyclin H, cdk7 and mnat1 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the TFB4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000477">
        <property type="protein sequence ID" value="KAF2070545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UX71"/>
    <dbReference type="OrthoDB" id="17307at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004600">
        <property type="entry name" value="TFIIH_Tfb4/GTF2H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12831:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12831">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIH TFIIH , POLYPEPTIDE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03850">
        <property type="entry name" value="Tfb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2070545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="318" mass="35745" checksum="DEB8A6FB91582303" modified="2022-05-25" version="1">MENNSIANNIDEDDDTSLLVIIVDCNIYSWGNREKLLKEGTLNDEKKTYITFSKFLEHLMVFINAYLMLNQENQLSVLCSKIGKSEFLFPNHTANDPLYSEKTIQEQILHKLQNMDLNNINETEERNISSSFSAAMSMALCYISRIKKATPTIRPRIVVFNVSPDVSAQYISVMNCIFSAQKQSIPVDCCILSQTDSTFLQQASHLTNGVYLKPQNQQQLTQYLLSTFLIDTYSRKFLAYPTLKSVDYRASCFCHKKIVDIGFVCSVCLSIFCKYSSSCSTCGTKFSLKIELPKKTTTANNTTTVIGGGNQSATASTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXC9</accession>
    <name>A0A8J4UXC9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008371</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000509">
        <property type="protein sequence ID" value="KAF2070318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXC9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="109" mass="12526" checksum="22E4F35486B5277A" modified="2022-05-25" version="1" fragment="single">MADISNKILEWSLHPLADKCEYIAHFKEGKLYPSEITFIVTSMCINVSNDSNGNPDMKQLPHVPDHITTLSINFNNNRVFKSNDKKRSLVGKQQQQPNVKKRKQDNDND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZ07</accession>
    <name>A0A8J4UZ07_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="5">PPIase</shortName>
            <ecNumber evidence="5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_006376</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclophilin-type PPIase family. PPIL3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="5"/>
    <dbReference type="EMBL" id="AJWJ01000292">
        <property type="protein sequence ID" value="KAF2072298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZ07"/>
    <dbReference type="OrthoDB" id="271386at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01928">
        <property type="entry name" value="Cyclophilin_PPIL3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000012">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF2">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="6">
        <location>
            <begin position="6"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2072298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="160" mass="17762" checksum="41065BF606BF792B" modified="2022-05-25" version="1">MSVTLHTSLGDIKIEIHCDSVPLAAENFLALCASNYYDGTIFHRNIKGFMIQGGDPTGTGRGGESIWKRQFKDEFPPHLKHNVRGIVSMANSGPDSNGSQFFITYQKQRSLNKLYTVFGKVISGLEVLDLMEKVQVDENDRPLNEIVLKSVTIHANPLAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZ21</accession>
    <name>A0A8J4UZ21_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_006328</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000287">
        <property type="protein sequence ID" value="KAF2072358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZ21"/>
    <dbReference type="OrthoDB" id="17904at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173:SF27">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173">
        <property type="entry name" value="ANKYRIN REPEAT CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="F-box" evidence="5">
        <location>
            <begin position="140"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="387"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="420"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="453"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="486"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="520"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="557"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="590"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="250"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="24"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="250"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="290"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2072358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="660" mass="72656" checksum="3B1A94016684E8A7" modified="2022-05-25" version="1">MNSSSSNSSSNSNNNNENNSSNNVTVSQNIPISPSLLSNSTGSRKIKASTSPSSPTLHSFSPLTKISNFFSNNSSLSPRSPKNKSSSALKELDMSKLNLSDNASLNNYNNNIINNSSSNNEDLILYDKNNNNSNSSITYYFDFFGLPIEIRMHILSFSDAVDLSKGCTVSKSWKEMVDDEQLWNNLNKRIFGDSQNTQSCWKKTVILNLGQLKDLENDLDGRLLWACENGHDQILQNAFEKRLTAINSPPFSLNSSSNQNNVYNNETDNNNNNNNSNLNHSNQTTPILYPHHHNHHHHHHNEDNNNNSNNNNNNNNNNNNQNNDNEVDLEMEEMDIAGPTSSPTINDHGISMKKRNKFLLKASYKGHKAVVECLLKNGSDIETNTRSGFTALHMACGKGHVDAAACLLKYNARIECKNKNGSTPLHTAAQKGHVRVVELLINHAASIEATNSNGVTPLNSAAHNGHTEVVECLLQRGANMEAINKNGITPLYSAAHRGHYKVVECLLKYNANIEGTTKNHGATPLYISAQEGYTDIVNLLLEHNANVEAKIRSGMRCGATPLYTASHRGHLQIVELLLRFSANTQVTDRNGSTPLHKASAEGHIDVVECLLKNNADVRAKSKDGTLPIDMAHKSVKKILKQYDSPKNGNTSGALSECLIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZ34</accession>
    <name>A0A8J4UZ34_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="14">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_006276</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="14"/>
    <dbReference type="EMBL" id="AJWJ01000282">
        <property type="protein sequence ID" value="KAF2072418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="270392at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02735">
        <property type="entry name" value="RNAP_I_Rpa1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01435">
        <property type="entry name" value="RNAP_I_RPA1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000019">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.390:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.180:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.120:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047107">
        <property type="entry name" value="DNA-dir_RNA_pol1_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015699">
        <property type="entry name" value="DNA-dir_RNA_pol1_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF11">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="16">
        <location>
            <begin position="324"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="156"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1298"/>
            <end position="1411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="159"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="1345"/>
            <end position="1366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="1385"/>
            <end position="1400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2072418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1616" mass="181569" checksum="7805AF250E1FA703" modified="2022-05-25" version="1">MSFQNSNLIKNEISGVSFQFYTSEDIRKLSVKQIMEVESFDMLGGPVPSGLHDPALGPTGPPERCSVCSLDYIECPGHFGHIELAIPCYNPVIFTVLFKLLQSKCFNCHSFKHGKDTIKDYVDELKLLFKGDVVGAKKLRDLNAVKKLASGFKAKAEEEKNESDDDDEDEEMKDSDDFLNGGSQDKYVSEKKTTHFEEARRSVLKEFLGSNTAYGLPCNNCQGYSPRLRNGDASKSKVFIMPLSSRYKAKNENLANKGIKMKTKFSSVDEETEHGTWFAPWEIESHMKKLFENEKEILDLLFGHLVVNTSGNGKERFTKESNVESFFMRVFPVTPSRYRPPNFVNGRRGEHPQNNHYKGMMKCNRLIKSCIDSGEGKAKNLVNAVCDLQMHVNNMYDNSKSASLVNPVANGIKQILEKKEGLFRKHMMGKRVNYAARTVISPDISLETNEMGVPEYFAKTLTFPQPVTSFNFNQLAQAVINGPDKYPGANFIEDENGFLINLSKESHEKRVALSKTLLTSRPHAPRGINKKVYRHLLNGDYVLANRQPTLHKPGIMGHRVKVLGKNEKTLRMHYCNCSTYNADFDGDEMNIHFPQSLLASAEIREIAANNYQYLVPRNGAPIRGLIQDHILTGVLLTKRDTLFTKSEFQSILYASCWAINTKHPIQTPPPCIVKPMPLWSGKQLISAALNHLTIGRVQLNLEAPSKIPTKMWGLTGPEITRDSMVIIRDNEMLAGILDKGHFGAASYGLVHTCYELYDPDVAGSLLTLLGRMFTNYLSTRGFTCGVDDLLMKAKEEKFRAESLKKANEEGYTVATKFANAKEYNEIESREFLGKALQSEREVARLDGMLKKALNTYTSKIIDTLIPGGQRKAFPYNNFSLMTVSGAKGSVVNFSQVSCLLGQQELEGKRVPRMVSGKTLPSFQPYDPSARAGGFVMDRFLTGVRPQDYFFHCMAGREGLIDTAVKTSRSGYLQRCLIKHLEGLSVQYDNTVRDADGSVVQFNYGEDSLEIAKTPYLTKFQNIAENFNLFKSQFPYEQLVEQLSNPKVQQYIIDLKDDPELDPLMSKFSPSSDLGCVSESFLAALNKYVEQNPQKLIQTPTNKSGKIQEKDFRNIMYLYFNRSMVSPGEAVGLLCSQSIGEPSTQMTLNTFHLAGRGEANVTLGIPRLREIIMTASREPLTPLMEFQLNDPTDKVKTEKLAKYLELLRMSDLINEITVQESTKDIERHYDVEIQFTKNLKSIFELHRIESNADLTRIYESFIKAINRKVVRTTKMKNPDIGKGSKQKSDIDDEGQIEFQDESSTNDNHQDENANDSGSKKKKGTISNDDDSATAKQKNKKKQQVSYDEDEEQEDQEEQDSADEDDGSDSEKKKKKKKKSSTSSDSESSDESDDKDQQDSDSDSTNKNDSKSSAFNMKFNQSHLSFTITVATDCKKVLMLGIVEEEALKCVIKSCKGITRTFINEKQNNGVTEYSIQTEGVNLRELFQMKDKLKIDQLYTNDIGSILSKYGVEACRKAIVSEISNVFGAYGISVDKRHLTLLADYMTFEGGYKALNRNGIENNTSPFQKMSFETTFQFLARSTLMGDSDNVISPSSRIVLGQVVKSGTGCFEIVAPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0E6</accession>
    <name>A0A8J4V0E6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF962 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009559</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000741">
        <property type="protein sequence ID" value="KAF2069122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0E6"/>
    <dbReference type="OrthoDB" id="5511466at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009305">
        <property type="entry name" value="Mpo1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34205:SF2">
        <property type="entry name" value="DUF962 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34205">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06127">
        <property type="entry name" value="Mpo1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="105" mass="12487" checksum="D1727D14D5B633D4" modified="2022-05-25" version="1">MGNQDLVLYTTFEDFYPFYLKEHSNRTNRRLHFVGTSLGLLSLAYFFFTGQFKLLFVPFLCGYGFAWVGHFFFQKNKPATFKYPLFSLRGDFKMWFSMLTGKIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0J0</accession>
    <name>A0A8J4V0J0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004208</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000144">
        <property type="protein sequence ID" value="KAF2074503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0J0"/>
    <dbReference type="OrthoDB" id="60283at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052050">
        <property type="entry name" value="SecEffector_AnkRepeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586:SF3">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="729" mass="85407" checksum="C1DE77BD2A0D673E" modified="2022-05-25" version="1">MNIDQLFRNVYNNKFLNRNIQYFIKLNRVTRKYDDLCGVNFMIQNKYWCLLKDKLKRKEYLAITPHQKNIIFSIPDFDLFLMVFNRFKHYYPVAYPPLLQEATKKDNLEILVYLHKLGYKGTMNVCLDDAIRNKNSAMVRYLLEKFDYCLVAENKFFGMILNFATEINDLEMVKYMLKNKQQHMTFDKLEVALQVACANGSLEIFQLLYSTRGNMTPFLYWFTTTICHFEMFMYLLHTFKSSIKQEWYIEIAKTCKYKSHEIVLYLLQHELIDQDTWNTVKTTIALDAFKNNDMQVYQQFKDSDSRLKTLYLSSVKGATDSLERVKELLSMGLMVDIYSVSMASKNPNSPEILEYLWRQEFCSTFSLNLFVSKIGGVVKCNQYLLLQFLIKNQYDLSKEQWWWCDMAKVDRNLPFIQLFLSKYPPDDQMRVAHLSNGLSEAATFNSINTFKYLYDLLLLESDQSPQLYSEAYKKAVANSNIDIIEHFIQKNTPHDNCVLGYSRSVEIAELLIKSQTHTINEQTIPTIVGDSNDLHVLKYLMELFPKSNQDLVWSINLAIRRSVSAGKFEHFKYLLAHSNNIHDQSILKTIGTFGNLQMLDYYVQYRNSKEPKPSIDFYPCFCQTIQVGHLELMKYIIDKQGIEMVVNDNLIYKIALTGHSYLIEYLKEPLSKCNINFLYSLKLSKTMYVSGGQEEYNHYLQVVSDDIISKCLSNASSGNKKGFFSFFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V118</accession>
    <name>A0A8J4V118_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002126</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000056">
        <property type="protein sequence ID" value="KAF2076575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V118"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="135" mass="14820" checksum="818E45C54D52534D" modified="2022-05-25" version="1">MDNSSNNKINGNKDHSIIVAEITSTQPSEIQQGQGTPIESCVFNITVSNHADVKRFDQQNFDPAQLAEILSEVAPPAHTFDGQVDGEQEDDDGNTPMKMQFEGVFGVPSYEGYEPNHLMGNIGWIPVLPHTTTHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V137</accession>
    <name>A0A8J4V137_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein uS2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_003498</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (25S, 5.8S and 5S). Interacts with ribosomal protein S21.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000110">
        <property type="protein sequence ID" value="KAF2075193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V137"/>
    <dbReference type="OrthoDB" id="414863at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000012">
        <property type="entry name" value="40S ribosomal protein SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03015">
        <property type="entry name" value="Ribosomal_S2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_uS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_uS2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027498">
        <property type="entry name" value="Ribosomal_uS2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005707">
        <property type="entry name" value="Ribosomal_uS2_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01012">
        <property type="entry name" value="uS2_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11489">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00963">
        <property type="entry name" value="RIBOSOMAL_S2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2075193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="251" mass="28061" checksum="E36369DC0080535C" modified="2022-05-25" version="1">MSQFPAALNPHQDDILKMLACQTHIGGNNVDKDMESYIWKRKDNGTFIINLAKTWEKIQLAARVIVAVENPADIAIISAKPLGQRAVLKFANFVGATAFSGRFTPGTFTNQIQSKFTEPRLLIVADPITDKQPLMEASYVNIPTIALCDTFSRLDGVDIAIPCNNRGKNSIALMFWLLAREVLFLRGVLPRNTTWTVKVDLFIYRDSDEDPAEKEKEKELKQIAQVATPVVENVEFTNLQAAPINDWAAQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1F4</accession>
    <name>A0A8J4V1F4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ketosynthase family 3 (KS3) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_010590</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001221">
        <property type="protein sequence ID" value="KAF2068084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1F4"/>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050444">
        <property type="entry name" value="Polyketide_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45681:SF6">
        <property type="entry name" value="POLYKETIDE SYNTHASE 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45681">
        <property type="entry name" value="POLYKETIDE SYNTHASE 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="4">
        <location>
            <begin position="8"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="156" mass="17523" checksum="E41E840A3CA796F7" modified="2022-05-25" version="1" fragment="single">MNYSVGNKNKVAVIGIGLRLPGKSNSPQEFWNNIKNKFDGVVEIPKDRLSEKFYNIGYIANNKGGFIDLNEWLSFDPLFFGIKPSDSETMDPQQRMLLKCTYEALEDAHIKPIDIRGSNTSVFVGCCSLDYKAQLVNDGPLLQQETSYHALANRVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1K2</accession>
    <name>A0A8J4V1K2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001496</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000036">
        <property type="protein sequence ID" value="KAF2077175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1K2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="252" mass="28451" checksum="26978FA860E460CE" modified="2022-05-25" version="1">MTHNLANSVFLGASLVGALAVAGSYYYHLHTSTQGSPSSVDESKIYESIDKYIISRIPNVPDRDVVSVLLTTRTRNLDTVPQTLVNRIAQYLYKSLKSHIPSLTHVNVLNIDLDDLPCYRQDLEGYQSKIDAIISLDDSLASYDSYATEHLFKTFEKIHSFLRSSSPTLCIFYHSTNQPQSKLTSSSSLLIPNWNHYNISSNKILFEMSGFKVHSIKTEPISTPVHNNSNQISFISLTSPEWEINKKDDLKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1N1</accession>
    <name>A0A8J4V1N1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CENP-V/GFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_001405</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfa family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000033">
        <property type="protein sequence ID" value="KAF2077280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1N1"/>
    <dbReference type="OrthoDB" id="15219at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1590.10">
        <property type="entry name" value="glutathione-dependent formaldehyde- activating enzyme (gfa)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006913">
        <property type="entry name" value="CENP-V/GFA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051052">
        <property type="entry name" value="Diverse_substrate_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942">
        <property type="entry name" value="METHYLTRANSF_11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942:SF4">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04828">
        <property type="entry name" value="GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51891">
        <property type="entry name" value="CENP_V_GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CENP-V/GFA" evidence="7">
        <location>
            <begin position="3"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2077280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="432" mass="49039" checksum="9D55444A710DC34C" modified="2022-05-25" version="1">MDRKGSCACGKVKVTCEISNEECGVCHCGTCVKWSAGPFISIKGVKNVNFVEGEQYVKLFDSSFWAQRGFCSECGTSLFYQLKQSKEYIMSLFLFDDMVKPQLISQVYIDKKPSCYEFSNQTSINLTEAQIIAMFAPKNFKFNQSFYLNMSTSSNQESNLHPVAKGYHDKDTTDSYKKGRPTIPLETVTFIKDTFNLDPNVKALDLAAGTGKFTELIAKGGFKDISAIEPSPQFRDACVEVLENIKSSMSPDLVYQVEDGLSTSIPRGDESVDVLFISQAFHWFDSVESIVEISRVLKKGGVLAMVWADMDNSVPLTKALTDIFHEKYYDGKAPQYRSGKWKKVFEDLKSNPKPNTIDPELKLKTFYFNHHLNKEMMIDRAMSTSYIAMKTKEEKEQISKELAEVMDKDPRSSDGKYFDLKYDVCLYYTTKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1U5</accession>
    <name>A0A8J4V1U5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_001204</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000027">
        <property type="protein sequence ID" value="KAF2077505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1U5"/>
    <dbReference type="OrthoDB" id="10264639at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14867">
        <property type="entry name" value="uS7_Eukaryote"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.455.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="entry name" value="Ribosomal protein S7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_uS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020606">
        <property type="entry name" value="Ribosomal_uS7_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_uS7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_uS7_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005716">
        <property type="entry name" value="Ribosomal_uS7_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003106">
        <property type="entry name" value="PRK04027.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01028">
        <property type="entry name" value="uS7_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="Ribosomal protein S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00052">
        <property type="entry name" value="RIBOSOMAL_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS7" evidence="7">
        <location>
            <begin position="30"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2077505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="190" mass="21385" checksum="681913A054468224" modified="2022-05-25" version="1">MVAEVTLFGKWSYEGVLCPDISLQDYICIKKNVFLPHSAGRYNKIRFRKSQCPIVERLANSMMMFGRNAGKKVMAVRIIEQAFEIIHLLTDKNPLQVLVEAVMNSGPREDSTRIGSAGTVRRQSVDVSPMRRVNHAVYLLTQGCRAAAFRNIRTVAECLADELINASKNSPNSYSIKQKDALERTAKSHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2V2</accession>
    <name>A0A8J4V2V2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">COMM domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_006732</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Scaffold protein in the commander complex that is essential for endosomal recycling of transmembrane cargos; the commander complex is composed of the CCC subcomplex and the retriever subcomplex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the COMM domain-containing protein 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000323">
        <property type="protein sequence ID" value="KAF2071957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2V2"/>
    <dbReference type="OrthoDB" id="10251426at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055070">
        <property type="term" value="P:copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000009">
        <property type="term" value="P:negative regulation of protein localization to cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902306">
        <property type="term" value="P:negative regulation of sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031398">
        <property type="term" value="P:positive regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032434">
        <property type="term" value="P:regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017920">
        <property type="entry name" value="COMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033776">
        <property type="entry name" value="COMMD1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037351">
        <property type="entry name" value="Murr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21199">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21199:SF1">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07258">
        <property type="entry name" value="COMM_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17221">
        <property type="entry name" value="COMMD1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51269">
        <property type="entry name" value="COMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COMM" evidence="5">
        <location>
            <begin position="108"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="151"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00016551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00093300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00093455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2071957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="182" mass="21568" checksum="D6AFE44BA0819403" modified="2022-05-25" version="1">MNNPKLFSNILGFLLNKEYEDDTLTIETLHEMVFSETDVSLENVEAMYDKCKQVLKKAIYLDHNITSFETLVKEDFTDVQQECFIKFWKINKKKIHEIIYKKTRFNNSLDKINWRIDLKTKSKNVNEMNEPVSIVELKLKNNNNKLNSNNIIKFEMDKSQLEETLQQINNIQKHLQSKSMAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V332</accession>
    <name>A0A8J4V332_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glucosidase II subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_009321</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycan metabolism; N-glycan metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000679">
        <property type="protein sequence ID" value="KAF2069359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V332"/>
    <dbReference type="OrthoDB" id="3237269at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090599">
        <property type="term" value="F:alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06603">
        <property type="entry name" value="GH31_GANC_GANAB_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14752">
        <property type="entry name" value="GH31_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000046">
        <property type="entry name" value="Glucosidase alpha, neutral C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000039">
        <property type="entry name" value="Glucosidase, alpha neutral C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000023">
        <property type="entry name" value="neutral alpha-glucosidase AB isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030458">
        <property type="entry name" value="Glyco_hydro_31_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025887">
        <property type="entry name" value="Glyco_hydro_31_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762">
        <property type="entry name" value="ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF54">
        <property type="entry name" value="BCDNA.GH04962"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13802">
        <property type="entry name" value="Gal_mutarotas_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00129">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035175723" description="Glucosidase II subunit alpha" evidence="12">
        <location>
            <begin position="34"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 N-terminal" evidence="14">
        <location>
            <begin position="93"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="13">
        <location>
            <begin position="395"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="15">
        <location>
            <begin position="731"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="203"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2069359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="951" mass="109609" checksum="663BAF15FA4CC551" modified="2022-05-25" version="1">MIKKQERKSSSLMPTFCVIFFVFLVTMTSLVESVDHSKFKTCGDSNFCKRNRINHENNKEGFYRLTSIQLSDDKHSILGDIVQNNNEAQPLSLKIQVYKDRMVRVTVVEKNPLANKKRYHVQDVLLPTISTLPLEWVEKPNDKQFSFTYESSQADSQTKKNNRVQVQINPFVLDLYVNGELSISTNDKGLFTFEHMREKIAQIDKPAPEEIKQQEQVDLNKEEGEQQDQQQEEKPTTNEVAGLWEENFSGHQDSKPHGPMAIGLDFTFAGSQTIYGIPEHSTSLALKTTKGEGINEQPYRLYNLDVFEYEIDKTMALYGAVPLIISHDTSKTVALFWLNAAETFVDIEDVVDKQNRKSKNTHWISETGAMDFFLLAGPTPTDIFKQYATLTGTTALPQLFSLGYHQCRWNYKSEDDVKDVDEKFDENNIPYDVIWLDIEHTDGKRYFTWDKNNFPTPADMQNIIGAKQRKMVTIVDPHIKRDNNYYIHSEATSKGYYVKNKDNNDYEGWCWPGSSSYLDFTSKEVRSWWSSNFAYDRYKDSTPNLYIWNDMNEPSVFNGPEVSMHKDAKHHGDVEHRDIHNLYGFYYHMATADGLVQRNKEQNDRSFVLSRAFFAGSQRIGAIWTGDNAAQWSHLEISNPMLLSLNLAGITFSGADVGGFFGNPEPELLARWYQAGAFQPFFRGHAHIDSRRREPWLFGEPYTSVIREAIVKRYSYLPLWYTTFYQNTLTGAPVMRPLWVEFPKEEALFKIDDQFMVGDSLLVKPVTKQGQTTTSVILPGKDTKQVWYDVDTGKQYPASTIEVETPLEKIPVYQRGGSVIPKKERIRRSSSQMKHDPYTIQIALSTDQSAYGELFVDDEHSFAYQKGDFIYKSFTFKDNVLTYKDVNKSSSFKSNNKIEKIVIYGLKSVPKTIVAASGDRKLEFEYDKEHSRLIIRKPELSIDNNWTLSFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3Y0</accession>
    <name>A0A8J4V3Y0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ABC transporter C family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_005668</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000232">
        <property type="protein sequence ID" value="KAF2073022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="29192at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030587">
        <property type="term" value="P:sorocarp development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18579">
        <property type="entry name" value="ABC_6TM_ABCC_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18580">
        <property type="entry name" value="ABC_6TM_ABCC_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002822">
        <property type="entry name" value="ABC transporter C family member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000010">
        <property type="entry name" value="Multidrug resistance-associated ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000610">
        <property type="entry name" value="Multidrug resistance-associated ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044746">
        <property type="entry name" value="ABCC_6TM_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044726">
        <property type="entry name" value="ABCC_6TM_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF172">
        <property type="entry name" value="ABC TRANSPORTER C FAMILY MEMBER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="227"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="344"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="745"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="786"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="866"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="889"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="973"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="104"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="449"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="768"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="1067"/>
            <end position="1301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2073022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1311" mass="148291" checksum="58DA8A585C270475" modified="2022-05-25" version="1">MNFLRSRLASYFKLTDYQDVNDSFSKECPENNASLISKITFQWIQKLLFKGYFHGPLQLDDISDLPDSLKTSHSFNLLKGMSLDSKYSFLLYVYKTFGLEHKSVIVFKIISLVFSILTPIILNLFLTFVSQANKNIYQGWGYCLGLFFSAMILSVSLQQANWRGLRLVLQIRGSLSTLIFKKMFLLSNTAKRKFKFGSIVNLMLDCDFFQLYFWSSYLDIILFPIEIISLIILLCFVIGPSGLIGFVIMGLSIPATTLSSSKMTKFLRLSMAENDNRIDLISELIGGIRFLKQFGWESVFTNKIQDVRAKQLKILYTRILFWILNQMIVQSTNALVLLSTFSVYILLGNELSASVAFTALTIFVNLRKPMENFPSALQSLLGLIPCSQRMRDYFTAEEIQNPPFNLLDQHQSLDFSLKKVDITNGDVEIENGSFDWEDGAGQKMEKTSSESDNLLEMSSQEPKKSNNVLSNISFNAPKGKLTIICGQVGSGKTSLVSALIGEIYKVDGRISKPESMAFTPQQAFLVSASLRENILFGKPYDKERYAQVIYACSLDTDLLQLPGKDLTEIGERGINLSGGQKQRISLARALYSDAECFILDEPLSAVDPEVGKHLFDHCIQGMMQGKTRILVTHQLQFIPSADHIVVVQDGNLIQGTYSQLQQLGIDFESIMKTKKLNIEPENQDQGKKPDLLNNESKQASIESIISDENDPDIVEKSKLLVKEDRNEGSVSLSVYLEYLSAGGPLWFFFTFVVGLYLLSQVLFQGSEYWISIWTQRVIQPDPGNKFYLLIYLYFIGGFIVLLGLRYFLLSRHTFKSTANLHNELLNSVAYASCQFFDQNPSGRIINRFSRDVSDIDYSLTEMLSSFIYLNCSVLVSLVIMVFLNPFIVIPFFVLSIIYFIIQRIYVESSRELKRMESIARSPVYNTFAETFNGVTTIRAFSQQQRFITDLESRLNIALRLIFYSYSIHRWCEVRMEVLASVIVFFSSIFTLYNSSTNAGATGLAITTALSLTGLLNRTIKNFTDIELKMNSVERLKSYIDTPREGGEDQPTFNPKAIGNNWPQSGSIEYRNVEIRYRADLEPSLSDISFSIKNNEKIGIVGRTGAGKSTIGVSLLRMVETTKGSIFIDGVDISKIRLHDLRSRLGIIPQDPFIFSGTIRANLDPYEQYTDQEIWEALEKVQMNTVVQNMSDKLNSAISVNGEGLSVGQKQLLCLARALLRNSKIVLMDEATASLDYETDYIIKQTVKNNFQNSTVLTIAHRLDTIIDSDKILVIDKGQMVEFDSPQNLVNNPNSRFAQIVNSQTSFLYNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4F2</accession>
    <name>A0A8J4V4F2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cation-transporting P-type ATPase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">CYY_001231</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000028">
        <property type="protein sequence ID" value="KAF2077458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4F2"/>
    <dbReference type="OrthoDB" id="158672at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005391">
        <property type="term" value="F:P-type sodium:potassium-exchanging transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000003">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000083">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000095">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050510">
        <property type="entry name" value="Cation_transp_ATPase_P-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005775">
        <property type="entry name" value="P-type_ATPase_IIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01106">
        <property type="entry name" value="ATPase-IIC_X-K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294:SF21">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294">
        <property type="entry name" value="SODIUM/POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00121">
        <property type="entry name" value="NAKATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-1278">Translocase</keyword>
    <keyword evidence="7 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="227"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="418"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="444"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="966"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1077"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1114"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="17">
        <location>
            <begin position="166"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="34"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF2077458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1148" mass="127218" checksum="7C22B282CF5B734C" modified="2022-05-25" version="1">MSSLRNSKSSVDFNVSNTDPHQNNNNDNGINPFADEHHYEEKKQYTEIELGTSGQISKSRSTEMNGLGISTSSMKKSKSIEGGLGASTSSMKKSKSMDSVEFIQEPQIPLYFSMVKEKERRRELKLRKRIKKKFKKGPKPSVSSPESASALANASVIESIPSDLTSDHAISIEDLASKYQTNINFQNPKNSYGLSSEKAEEFLERDGRNALTPTKPTPKWVKYVKEYLGLFPLMLEIGGILSFIAYGLDKEVGGDSLYLGIILWIVVIVTCTFSYFQTAKSASVMEGFKKLASSSTKVIRDGQLIEIDSEILVVGDIVVIRAGDKIPADIRIILSNHLKVDNSSLTGESEPQTRTPNCTDENPLETQNLAFYGTLACAGDAIGVIIATGDRTTIGKIASLASKSKPASTPMKREIQQFIKAISIVAITLGLTFLVIGFSRKVQWIYVVIFTIGVIVSQVPEGMLPTLTVTLNITAKRMARKNVLVKNLLTVETLGSTKTIASDKTGTLTQNIMTVVHLWYDETVYSCNSLAASNFFNTKSTTFQRLFMISALCNRTVFDKGTNQEDIPIQQRKTIGDASESALLKFCEQIENVEQFRDKYPKLFEIPFNSLNKWQLSVHEDTDGTLFLAMKGAPERIINICSKIMINGEEHDINSSHLDNFQKSYEFLAGKGERALGMAFLQLDPKIYTADYPFDADEKNFPTSGLVFIGLTTLMDPPRPGVPEAIQTCKEAGIRVMMVTGDHPLTAASIAKQVGIIEVDETLNDKAKAQNVDLFDLEFAPDQAVVIPGSMLDDLTHEQWDKILSLKQIVFARTSPEQKLIIVEKCQKRGDIVAVTGDGVNDSPALKKSDLGCAMGITGSEVAKEAASIVLLDDNFSSIVAGVEEGRIIFDKLKKSICYTLSSNIPEVLPFFCFFVLQMPVALSGILILCIDLGTDLLPVFSYAYEASESEMMKRKPRDVKKDRLVSLKLALFSYVWLGTVQCAAGFLNYFLTFRDYGFSPSDIYNVSSDYFMNTKAEPYQGSLPGAQVYDAKEQADILLKAQTAYFVAIVLTRVGAALSCKTRKNSIFVQGFRNNVFNAGIITMISLALIIVHVPGIRVFFGCTIISYKYWLLPLPFAVFLVTVNEIRLYMIRNLPKKSKFRKMIHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V524</accession>
    <name>A0A8J4V524_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Transcription initiation factor IIF subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_000999</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIF beta subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000022">
        <property type="protein sequence ID" value="KAF2077683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V524"/>
    <dbReference type="OrthoDB" id="26094at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005674">
        <property type="term" value="C:transcription factor TFIIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07980">
        <property type="entry name" value="TFIIF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000035">
        <property type="entry name" value="General transcription factor IIF subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003196">
        <property type="entry name" value="TFIIF_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040450">
        <property type="entry name" value="TFIIF_beta_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040504">
        <property type="entry name" value="TFIIF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011039">
        <property type="entry name" value="TFIIF_interaction"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10445">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10445:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02270">
        <property type="entry name" value="TFIIF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17683">
        <property type="entry name" value="TFIIF_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50916">
        <property type="entry name" value="Rap30/74 interaction domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TFIIF beta subunit N-terminal" evidence="8">
        <location>
            <begin position="20"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIF beta subunit HTH" evidence="7">
        <location>
            <begin position="170"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2077683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="27142" checksum="E5F1FC417AB6315A" modified="2022-05-25" version="1">MSEESNDALDGEINIDNADNQVWLMKVPKFLADHWAGSQGSIGKIRIKSGDNISLTIQVPGTTTTEEFQLITTPQNIENQPLKIFSEDAEGALALEGNIGLKCDIKMNLDSPTYRELMKTRTTAYNTKTRVAKTIDDNQPFLPPRPINPKIQVSTAVYAKKKPTTDKKERKSEDQVIDSIFDAFHDNNHLDIKTLVKYTDQPQTWLKTVLNKVCIFNKRGPNKNTYELKPEYKNKDEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V597</accession>
    <name>A0A8J4V597_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alpha-mannosidase</fullName>
            <ecNumber evidence="3">3.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_007000</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.</text>
            <dbReference type="EC" id="3.2.1.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.24" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000350">
        <property type="protein sequence ID" value="KAF2071684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10261055at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050843">
        <property type="entry name" value="Glycosyl_Hydrlase_38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607">
        <property type="entry name" value="ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607:SF3">
        <property type="entry name" value="LYSOSOMAL ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035310591" description="alpha-mannosidase" evidence="7">
        <location>
            <begin position="24"/>
            <end position="1351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1294"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 38 N-terminal" evidence="8">
        <location>
            <begin position="52"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1068"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1099"/>
            <end position="1112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2071684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1351" mass="155652" checksum="595C30AB3422499E" modified="2022-05-25" version="1">MKNFYFILFCFILFISRDHYVFSWIEDQPLNIFVVPFSLPINKDNLDLDTYYESNGKHIISSIVDNLVKDHERKFIWPELFYFRKWWNDPQFKSKHSIVKQLIQNKQIEFVNNGIYLNENYNNSYQYILNDLKEGSEWIVNNLGGPVVKSFLNIHSNGHSEAFSYFLSLSGIENIVLQKINRDLKNQLKSEKHLEFFWQDSMITRNQSNNEPFKLFTHILPDQDIDIKHTCGPNSEICQYFDFYNNDHKITHSNIQMMGDNLLQQYKSKALLYRSNCLLILLGGENMYKDVSKTNDLLDKYQQLFNYIKSSNENINIRFSTLGEYFDNLKQSRLPINLAPAKSFSNIDIILQDQYKNGQSLSQLVPGQQIIPDTRDFLIFKGDFFGISNDKNQYLSFSSNQQDNDLDLKLNNLLKVLNNLLSLDSSLDFQSKQVAFNQIEQCKSTPIMYKNQCINDMEGLLSSLTSQSLKCNSNLNLTNSNEIIPISLIDTYSVVLFNSLELKKEELVSIFIDSPYVELYNENQSPVLIQISPIWNDTINTLIDNRFLLSFIVEIQPFSTITYTLHLNMDQKEKKSQSLYYSQSTILSILGSTPKSFKSINPFVINFARLGSQELVLDNNFFKIKLNHLNGLFETIVINNGGKNKMDIINFEESHWLYQSESGHYQNEWIENGNLIKSEISNSLIRIVKGPIFNEIIIFKNSNSRSSLQSISIKLLNCLDPFKKECIDGSKMIKYESTFNQFKSKQVGDNILRFSIKSNMNINNNNLNINDISNLNHLKDKELKKNNIYFSDSNGFSLIPRKSNIFGSNSYHSQFIPMTSVAYFGQHYSVSSGLKAGVFCKNMLGITSIKDGSLDLMLNKFDSLKNRDELNIVSLWLSFNDIFSTSFPFSKSNHLKSPIQVFSFPNSYTTNKCTFNHFIPTIKIPNNLQFISLHSQFNSNNKDDGVICHLKLLNVENSIKSTLALKNQLQISSLFPNHEILNVVQGNLQMTKVFDISTKTSIRLTIGELVSLSFYLKELENDLIVIDNQIPSTLVKNDESGAGSKSLSRPVIDASSKPFKEVEKRLKGSPKFSSFSPESNHLPPKYNSFNLRKNDNLKNIDNNNNNNNNNNQIDQINNNEKIDSENESEFINQQTNHEVKSKKTSISIEIDKLKQEFQKKTGNLIFKNNKNNNNNLEKEITIKPKARLDRKAGLSALELQILQLEKDFELEKVIIKAQMKQLKKKPINPAIVYSKQYALDYIIKQEKTLLEIKEQISNNPGLLNKPLEEHTDGALVLGKKIPEMPKKYSGSNQFYLLVILLCFSCITYLLFYLTRRQRKPLSILQNQHQLSTSSSSSSTSIIIDIEDNKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V772</accession>
    <name>A0A8J4V772_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002113</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000056">
        <property type="protein sequence ID" value="KAF2076562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V772"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="210" mass="23588" checksum="D3E8FECA412E1AE0" modified="2022-05-25" version="1">MSGSADSAYDQLCSNFKLDINDKNQDMDLGGGSGGSEIRGYGSSCVVSDWEDGGSCGGAGFSPVHDQSFEVFNHRQNHNHKLDHIYKTCPYDYCGKVVYGGSENMTLHMQSHNTQQSHIEFYKSRAIQKLKNKYGNLALEKDYNDNYVCFFYGCQSRMKNHFDRHIAKHEARNDPLSIYLSPPIVPPNTNIHILKNANFFVNHFMGSGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V9X3</accession>
    <name>A0A8J4V9X3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_002110</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000056">
        <property type="protein sequence ID" value="KAF2076559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V9X3"/>
    <dbReference type="OrthoDB" id="515401at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="3">
        <location>
            <begin position="32"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="54"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="66"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2076559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="134" mass="14515" checksum="45F5670C0A75DCD4" modified="2022-05-25" version="1">MKHNTQCPSGPSTVVVVKRSRGRPRGPKPTHCTSCFVKTTPEWRKLNGKPVCNACGLRPRKAKEQSTSPESSPASSPSFTSSSTTPTTSTTASPTSSSPSTTTIQVIIDVPLIINTIREEKLKIQYILNRVNSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VBK9</accession>
    <name>A0A8J4VBK9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_000391</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000007">
        <property type="protein sequence ID" value="KAF2078299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VBK9"/>
    <dbReference type="OrthoDB" id="21277at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046621">
        <property type="term" value="P:negative regulation of organ growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16454">
        <property type="entry name" value="RING-H2_PA-TM-RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033276">
        <property type="entry name" value="BB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46400:SF5">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46400">
        <property type="entry name" value="RING/U-BOX SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="316"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="107"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="285"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="33"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="232"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="289"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2078299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="375" mass="44456" checksum="16DB72C4ED0DF372" modified="2022-05-25" version="1">MNPNSNPFRNNSNHSNHGTSGTMEPFYTTQQLFQHYHQINQQQQQQLQQLQQQLQQQQQHQLIQQHQQQIIQQHQQVHNQILHQYQQQQQQQQQQQPQQIFNQFRNQQQPLQSQQFQQHQQHHQQQQIAPPQQQQQQQQQQQQQQLQQQQQHQLQQQQQQQQLQQLQQRHSLAQNQALQMHLNAHQQVVHHQIHHHHHLHVHHHHHLHLNNGSTMININGPHTSTILFENNNDDDDYLEDSGDENNQFYGDEPSYETLLNLEAYNVPRGVPKKVLKKLNRYIYHKPQESNSNNNSSNNDNNSNSNNSNKQDEEKLCGICQETFEDGDQLISLPCNHEYCEDEIIKWLDTHNTCPLCRCVVSFDSSTSSTTSTSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJ32</accession>
    <name>A0A8J4PJ32_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">S-adenosylmethionine synthase</fullName>
            <ecNumber evidence="11">2.5.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_010577</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the formation of S-adenosylmethionine from methionine and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-methionine + ATP + H2O = S-adenosyl-L-methionine + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="11">Binds 1 potassium ion per subunit. The potassium ion interacts primarily with the substrate.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 2 magnesium ions per subunit. The magnesium ions interact primarily with the substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the AdoMet synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="11"/>
    <dbReference type="EMBL" id="AJWJ01001210">
        <property type="protein sequence ID" value="KAF2068097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJ32"/>
    <dbReference type="OrthoDB" id="5852090at2759"/>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18079">
        <property type="entry name" value="S-AdoMet_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000003">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000004">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000011">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022631">
        <property type="entry name" value="ADOMET_SYNTHASE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022630">
        <property type="entry name" value="S-AdoMet_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022629">
        <property type="entry name" value="S-AdoMet_synt_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022628">
        <property type="entry name" value="S-AdoMet_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002133">
        <property type="entry name" value="S-AdoMet_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022636">
        <property type="entry name" value="S-AdoMet_synthetase_sfam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01034">
        <property type="entry name" value="metK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11964">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02773">
        <property type="entry name" value="S-AdoMet_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02772">
        <property type="entry name" value="S-AdoMet_synt_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00438">
        <property type="entry name" value="S-AdoMet_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55973">
        <property type="entry name" value="S-adenosylmethionine synthetase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00376">
        <property type="entry name" value="ADOMET_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00377">
        <property type="entry name" value="ADOMET_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 11" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="10 11" id="KW-0630">Potassium</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S-adenosylmethionine synthetase N-terminal" evidence="13">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase central" evidence="14">
        <location>
            <begin position="65"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase C-terminal" evidence="15">
        <location>
            <begin position="188"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2068097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="334" mass="36431" checksum="9F205B9A4E0E2450" modified="2022-05-25" version="1">MVLGEITTKATIDYQKVVRDTIKKIGFDDSSKGFDYKTCNVLVAIEQQSPDIAQGVHIGKVNPEDIGAGDQGHMFGYATNETPELMPLTHFLASELVNKLTELRHNGTLAWARPDAKTQVTVEYKKEGGKLTPVRVHTVVISTQHDENVTNEQIRSELKEKVVKAVIPAQYLDENTILHLNPSGRFCIGGPQGDAGLTGRKIIIDSYGGWGAHGGGAFSGKDPTKVDRSGAYAARWIAKSLVAAGLADRALVQVSYAIGVAKPLSVFVDTYGTGKKSDEEILEIINNNFDLRPGCLIRDLDLLKPMYQITAAGGHFGRTHPEGFTWENVKKLNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJ97</accession>
    <name>A0A8J4PJ97_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010339</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001048">
        <property type="protein sequence ID" value="KAF2068335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJ97"/>
    <dbReference type="OrthoDB" id="26095at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053331">
        <property type="entry name" value="EGF-like_comC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24032">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035252354" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="704" mass="78108" checksum="B3DE09BDEC82CFFA" modified="2022-05-25" version="1" fragment="single">MRKIGFLFVLVLGLMMLINPASSLIQAQYNRLLEIRTSFNLETIFPEPIDFCSQLLIIQCVDGNSSVGFINIKGIGSSVIPSFTVFDRLTSLSISNATDSNDFFLNSDLYLPPSLLFLSCSYCGITKLPSNSSLTTLNLKNNPIAMISSGEYSITNETTVIMVNPDPLFADYLIKIKSDCEIVKLLFVNLGVMINEPTTYNCFLGYSSITLQIGPSFGPNTNLTTLINSTFPSLLNSRAQVLNLIGYPNIAYEPMYYFFSAQMNFINLELKTKQDEITPSQNVEITCDNSIVKTIDGGFLYNISKQASIAINSCGITKIYPEMFQSATFVSLKDNGITGPFPNYLYGKNQVLLDGNEFTGELDRKICENTQYSFSYNKLTGKLPSCFSCYLNSPAVRTKLIGNQFSNYNNDNIYPVCDTVVITSFANFSNVGSRIIGFDLPPVGGLISSNIPNITFTLTATNGHITVYYRTSNIPEIFKYKEFIVTIHGTRDFTFTFPVSKPNPQIFTHIPPTPYRDIGYSFTFIATAGIPIEMYLIKMDNSLPCEIISYDENKSTTSITCVVKSFSIPEKILRFTFETSNYPTIYYDVPFIRKFPVLASVSSVRKSGGLVTMYGLFGENLTNSNISQYNANIGSLDCNIKYLESSTIICLLGSSNLSGPQNVTITSFNIPSIFLNVFVFMEESKGCENSCSGHGFCNVNPSYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJA4</accession>
    <name>A0A8J4PJA4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_010308</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <location evidence="1">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001031">
        <property type="protein sequence ID" value="KAF2068365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJA4"/>
    <dbReference type="OrthoDB" id="44015at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03564">
        <property type="entry name" value="ANTH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.150:FF:000011">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014712">
        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048050">
        <property type="entry name" value="ANTH_N_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF5">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-BINDING CLATHRIN ASSEMBLY PROTEIN LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0168">Coated pit</keyword>
    <keyword evidence="2" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="6">
        <location>
            <begin position="16"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="279"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="348"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="641"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="362"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="389"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2068365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="665" mass="75189" checksum="F11C7243F15A3A63" modified="2022-05-25" version="1">MSNAWGKALDQANIVKASFNINDLEKSVIKGTRHKLRIPKEKHVRKLIIYTHDRLGPIGDLYLHLLRRLEQPDWIIVLKTLIVFHRIFAGGNIRFLEDLTHRGNIFPLGRFTDMTSTQAHQQSVFIRRYSAYLEEKVFSFKELRQEFDKDTFTSKGLSIEQLLNRIPKMQRQFDALLATHVEEVCDNIITINAFELLLKDSFKMYCNLNDAVLNILELYFNMTKRDATKALDIYKVFMRETDMLIEFFSSSRRKFHIDLPELSRAPSTVVNGLEEYLRDLDDSDMPPEDRYNNNNNNTGSRGRTYSGGNSNSHNNYSSIGSHSNNNTGSRNGQAQIQYKNEELISFDDDLFSSAPPPQTNSQKPIQQQPPQNLIQFDPLDPFQVTSGGNNNSFSNNNNNNNNMYSNNNNNNNNNNAVNNVPVLSNNYHNPFDPSYNEKALQIKAAFRSSIEMNKNNSVGSNNSMGSNNNMGFTLTPVNNQPFVMQQQPTPLMGNNNNNMFNSNNNNNNGMMPMNNGGMMPMNNGMNNGGGVLMPMNNGGMMPMNNGMNNGMNNGMNNGGMNRMGNNVGMMPMNNGGMMPMNNGMNNVGMMPMNNGGMMPMNNGMNNMNNGMNNGMNNGMNNMNNMNRGGMNMNNNNNNMNQGGMMFNPQQQQQQQQKPQIQNIYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJY3</accession>
    <name>A0A8J4PJY3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CN hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_010175</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbon-nitrogen hydrolase superfamily. BTD/VNN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000957">
        <property type="protein sequence ID" value="KAF2068499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJY3"/>
    <dbReference type="OrthoDB" id="10250282at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040154">
        <property type="entry name" value="Biotinidase/VNN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043957">
        <property type="entry name" value="Vanin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10609">
        <property type="entry name" value="BIOTINIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10609:SF27">
        <property type="entry name" value="CN HYDROLASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19018">
        <property type="entry name" value="Vanin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035269939" description="CN hydrolase domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="CN hydrolase" evidence="4">
        <location>
            <begin position="37"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="494" mass="54575" checksum="F5F254194EA1C353" modified="2022-05-25" version="1">MTLKINIIYCLFFLITLSSAIKISIGNNNNDDDGDYYTASVLEFSPSLIYSEKDIISNLKAFEYYANLASKNGSQIIVFPEYGIASGNFNTRDSILPYLEYIPDPKASNEPIIPCRNIEFKNRTILETLSCIAKQNNIVVVADMGDIQPCTNSSSTNDCPSDGRFQFNTLVAFSEAGELIARYHKSHLYNEPFFNPSPTPDPITFTTSFNVTFGMLICFDIMFGQPQTDLIQQYNIKNLVYATEWVNVNYAYAREIQQSWSRLNGANILAANIGMASFFSGSGIYSNGNVLNSFVNPTMKPMTQLLTAKVPKDPSQANIADKQQHSNHHHYQNNIVNELIRSPTSINLTMVPFEPNCQTDAPPITVTADNNGLHCQFTYSSPHMASGASSIPLFSLIAYSGNFNDFFNAQICAVSICSGSTADTCMNNVFDSSIYFDSVSIQGNFESGYHIAPTITTVPIANYFGDYKSGSNAITVSNITNPFLGVGLFSIEWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PK36</accession>
    <name>A0A8J4PK36_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carbohydrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010072</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000911">
        <property type="protein sequence ID" value="KAF2068604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PK36"/>
    <dbReference type="OrthoDB" id="5576763at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021862">
        <property type="entry name" value="DUF3472"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11958">
        <property type="entry name" value="DUF3472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035174296" description="Carbohydrate binding domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="261" mass="29176" checksum="A2E3AF8C7055B642" modified="2022-05-25" version="1">MKIVCLIIGFILATVVVSDPNINFNWGNNAGNIGYDKVVLPIKVLVAPTIGRTYYYALQCNFLGDDRSAIYMGIQPREPGKNLVIFSTFGTGTDSEHSQCVRGADGGSGTSCSVQYNWNLNELYYVSMQRVYMNVTHQSWEGYIVTEQGQSVLVGRFSVPSTRRGLQAGSYFFDEYFPFNGASKDPTKRKCIEYTKLSITAPLASFNNQWYPSTPTKIRVDTGKDSCAVYWNQNNARATETFLNGIYSYDLENGFLPPIPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL02</accession>
    <name>A0A8J4PL02_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_008900</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000596">
        <property type="protein sequence ID" value="KAF2069777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL02"/>
    <dbReference type="OrthoDB" id="2436667at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13156">
        <property type="entry name" value="KOW_RPL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000014">
        <property type="entry name" value="60S ribosomal protein L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000915">
        <property type="entry name" value="60S_ribosomal_eL6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049633">
        <property type="entry name" value="Ribosomal_eL6_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041997">
        <property type="entry name" value="Ribosomal_eL6_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10715">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10715:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01159">
        <property type="entry name" value="Ribosomal_L6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01170">
        <property type="entry name" value="RIBOSOMAL_L6E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2069777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="238" mass="26292" checksum="F4383FB55ED1D7E8" modified="2022-05-25" version="1">MARLENPSVAKGLKLFSSRAYANRTRTYLAKKNKVAAAKKVATVAPKKEKTFGKVKRVVAAKAPKYAPAIPAEVRAQASKVAPSKVALRKSIVPGTVLIIVAGRFAGKRVVFLKQLTSGLLLITGPFRLNGVPLRRIDQRYVIATSTKVDVSSVKVSEKVTDAYFAAEKKATKKTEDAFFQDEKKQVEKKKISETRVADQKSVDTAILAVLKKDKALTLYLKTKFFLRKGEFPHNLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL49</accession>
    <name>A0A8J4PL49_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GST C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_010536</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001178">
        <property type="protein sequence ID" value="KAF2068136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL49"/>
    <dbReference type="OrthoDB" id="14734at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03190">
        <property type="entry name" value="GST_C_Omega_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047047">
        <property type="entry name" value="GST_Omega-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016639">
        <property type="entry name" value="GST_Omega/GSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419:SF6">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE OMEGA-LIKE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419">
        <property type="entry name" value="GLUTATHIONYL-HYDROQUINONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015753">
        <property type="entry name" value="GST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01206">
        <property type="entry name" value="Xi.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01148">
        <property type="entry name" value="Xi_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST C-terminal" evidence="4">
        <location>
            <begin position="151"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="119"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="137"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="3">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="3">
        <location>
            <position position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="314" mass="36276" checksum="629024E8A4EAA174" modified="2022-05-25" version="1">MTTTTPKFDNGDQIDKKGSFNRKEACFRSLIAKDNPVFKPELDRYHLFISLTCPWAHRTLIVRNLKGLQHVIGLTVVDWQLGNDGWKFSERDGCTLDPLFGFAGIKEYYFKTDPQYSGRFTVPVLWDKKTNTIVNNESSIIIRIFNSAFNDFAKNPELNLYPDHLAKEIDEINDYIYPNINNGVYRTGFATTQEAYEDAFHILFQGLDKIEERLSKSRYLVGGTFTEADIRLFTTLIRFDAAYNGNFKCNRNQIKEFPNLSNYLRELYQMDIIKQTVNFKHIKGGYYSISSVNPNGIVPLGPNLSYLDIPHTRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLH5</accession>
    <name>A0A8J4PLH5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Calmodulin-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000740">
        <property type="protein sequence ID" value="KAF2069123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLH5"/>
    <dbReference type="OrthoDB" id="10394449at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="486" mass="54947" checksum="F6C8D1F46DD8F1B9" modified="2022-05-25" version="1">MCQDVSIRVNNEYLCDNQQYLSLFSNIDKVNHNIRISFLGNASDYLDINNSIRDLINDVELSISADFNLNEISDGVHNLFLKITNGNAALMGQLPDSITKILLFAVNPPIVQFNLISNLPSKLQELNFFFNVNCIQTRCVLPQSLTDLHYIGSYDSMKWLVVPPNKVYKKCEFVLDSNESFQWLLENKFIGKIHIGPGAIPLLKSHRIPPHVSDVYLNHVHDPDLLLPDTVERLYFSEFGTPFSQLSHLKELVIDNEYPIKLEKGVLPRSLETLSLYYNQPLEPDVFPNQLKTLYISEYNQPLCVNVLPSSLTDLTLGDFDQPLNAFVLPQKLKKLILHGFKQLTPDSLPVSLTDITLFKLTGSFDQCQPLDNLKKLQIGSLVPSVGRLLTNVKRLDLFVSNTAISKAAGTCLANTSIESLYLRVKYPSTLYPNSLPPTIKYLSLVNVVIKSDNVIPSSCRYLKSKSNHIDTKYVPKSVRYLKSIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLN8</accession>
    <name>A0A8J4PLN8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carbohydrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008417</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000516">
        <property type="protein sequence ID" value="KAF2070270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLN8"/>
    <dbReference type="OrthoDB" id="19156at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019028">
        <property type="entry name" value="CBM_49"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052879">
        <property type="entry name" value="Dd_Spore_Germination_Stalk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33239:SF11">
        <property type="entry name" value="CARBOHYDRATE BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33239">
        <property type="entry name" value="CELLULOSE-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09478">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01063">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035227793" description="Carbohydrate binding domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate binding" evidence="2">
        <location>
            <begin position="51"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="150" mass="16701" checksum="CBE4C3F0C2C2285C" modified="2022-05-25" version="1">MKSIQILCALLLTVAVANASFVCPKPCGANDICVMIDSNCKVLPWWSCQEVTMGVRMVGQWNDSVNNCIYSQWEVTITNHLPRKITNLFIATDDSLTLRDNSSIWNMAKNGNILSLPTYQPSVNPSSSYTFGFIIKGTHQPHMVIKAVTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLQ2</accession>
    <name>A0A8J4PLQ2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PIPK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_009345</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000684">
        <property type="protein sequence ID" value="KAF2069337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLQ2"/>
    <dbReference type="OrthoDB" id="19735at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00139">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF63">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="3">
        <location>
            <begin position="572"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="82"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="410"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="522"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="795"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="932"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="410"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="437"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="530"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="939"/>
            <end position="963"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2069337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1024" mass="116006" checksum="92A89B538669617E" modified="2022-05-25" version="1">MLSFSRFKSKGKSPTQNSSNTTTTTTTTDDGVNSQFYKNTITKKSLLQCKRIVIFGNKDGFLSNLLINVLSQYDSKASMVSITHSNNNNNNSNNNNNNNSNNNNNNNNNNIIEVLLEKQEIDKNTYKDVLIRNNDKDTLKDILKQIDIIVFCFEWCHFRDLYLEMTAVYNFCKSNDITPKHIIVTSPIGVEDSEFTTKNGSMESIFNPIPTSFLFYSSLMQWLFTGPPSFKNNNSNSNNNSNTNNNDTYNIKLPKNKNEEGCYWLDCDDLIKSIVEIITVIEVTETKLYFLTGKESLTCKEISNILFSECNIIVEDIDNSNNNNNNNNDSSLELQNKFFNYLQSPVSILQSPHLELFNIKATTFTNFLHRCQKSQLFTIFKHSKSRSNSVNSNNSNGILSGSSNSISIPLSSSPSSSSNSNSPSLNIQEKLPSNVITPSSSPLTTTTSATNSTPSAAPSSGYSRFVFNPFNFLKYSKDFIEETIYKSPTSSKRSDSLQSETQQYINLLTLAFRKMFDSEIQSSNDNNNVTSPSSSTTPPLLSSPPIDQFTNDDFTCETTFDFSIDPNVNNNSNNNCTDSPSPNIVVTTAGSINIQDSEIATTTTTTTTTTTTTAVTNNNNSNSNNNPDSMWSFTSFSPKVFKDIRRYFHIKDFLLSPKVNQQHKFDIVKFIEVKTIGRSGSFFFRSECNRYFIKTIPFSEYETFSKLFQSYYQHILKYNNTLLPRFYGVYRLKGKLKMPNSNNTDFYYQNRDTIFVVMSNLFYSEPKLDIKEKYDLKGSTIGRYVDVATLLNSSTSSNNSNNSDINDDNNNNHNNNNNNNNNINNLNNSEENVNSNNSNNNLQDLVFKDLNLKRKIMLGDFKKAFITQLNIDSEWMNSHGLCDYSLLIGLHISDKANINYIKLLSSNDQSNRDISFFKKDCFGIEASKPLNRNRSHSCSSAESNNNNSNNDNNNNDNNSNASNEDSDEGIIYFIGLIDILTTYNLKKRGENAIKGMIFDRTQISAINPRDYQTRFIKYIEKILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM26</accession>
    <name>A0A8J4PM26_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Malate dehydrogenase</fullName>
            <ecNumber evidence="3 10">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_009213</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible oxidation of malate to oxaloacetate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LDH/MDH superfamily. MDH type 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="3 10"/>
    <dbReference type="EMBL" id="AJWJ01000656">
        <property type="protein sequence ID" value="KAF2069468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM26"/>
    <dbReference type="OrthoDB" id="4069699at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01336">
        <property type="entry name" value="MDH_cytoplasmic_cytosolic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000010">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.110.10:FF:000002">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01517">
        <property type="entry name" value="Malate_dehydrog_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001252">
        <property type="entry name" value="Malate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011274">
        <property type="entry name" value="Malate_DH_NAD-dep_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010945">
        <property type="entry name" value="Malate_DH_type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01759">
        <property type="entry name" value="MalateDH-SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01758">
        <property type="entry name" value="MDH_euk_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003916">
        <property type="entry name" value="PRK05442.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23382">
        <property type="entry name" value="MALATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00068">
        <property type="entry name" value="MDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 8 10" id="KW-0520">NAD</keyword>
    <keyword evidence="4 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="12">
        <location>
            <begin position="8"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="13">
        <location>
            <begin position="158"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="131"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2069468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="333" mass="36115" checksum="7C2DBF0793CFB762" modified="2022-05-25" version="1">MSNSEIVNVVITGAAGQIAYSLIFNVIKGDMFGNDQKIKLVLLDIPAMIDGLRGVVMEIEDCAYPLVSSIVCTTDVKEAFTDAHYAILVGAMPRRDGMERSDLLKANAAIFKVQGKAINDYANKNVKVLVVGNPANTNALIASTCAPNIPKENFSCLTRLDHNRAKAQIALKANVNVKDVHNVIIWGNHSSTQYPDSRCGYINLPTGKTPISNVIKNDQWLQGEFITTVQKRGAAIISARKLSSAASAAKAITDHMHDWVFGTPEGEYVSMGVPSDGSYGIPEGIIFSFPVTIKNGKYTIVQGLPIDPFSRALIDSTHSELIQEKTSAFQFIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM85</accession>
    <name>A0A8J4PM85_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Isopentenyl phosphate kinase</fullName>
            <ecNumber evidence="2">2.7.4.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_008992</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>isopentenyl phosphate + ATP = isopentenyl diphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:33963"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:65078"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.26"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the isopentenyl phosphate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.26" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000614">
        <property type="protein sequence ID" value="KAF2069684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM85"/>
    <dbReference type="OrthoDB" id="1934954at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102043">
        <property type="term" value="F:isopentenyl phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04241">
        <property type="entry name" value="AAK_FomA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024192">
        <property type="entry name" value="Fosfomycin_R_FomA-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040647">
        <property type="entry name" value="IPPK_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654">
        <property type="entry name" value="GLUTAMATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654:SF1">
        <property type="entry name" value="ISOPENTENYL PHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016496">
        <property type="entry name" value="Kin_FomA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/glutamate/uridylate kinase" evidence="12">
        <location>
            <begin position="10"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="11">
        <location>
            <position position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR016496-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR016496-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2069684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="290" mass="32298" checksum="AA9BB52C1BAA3ACB" modified="2022-05-25" version="1">MNTDLDNNELLVIKFGGAYLCDKSKYQTLNDSNIDSFIEIIKHLVKGRFKLIVLHGAGSFGHHDAKKYKITSGFNKGEYQSDVGICKTRQSVLKLNALLISRLNSECINAVSVSPFDSWLTQDNKVIQDNCRHLELLLSMDIIPILHGDVCLDTKIGCTIISGDILIQTICEKLRPKRAIFITDVNGVYNLPPSSHPNAKLLSFIHLNENGDILNEQEQQQQQQFVHKQDSCDVTGGMEAKIKSAKTIASLYSVDTLIIGGTHHEIIQSQNLIQNVLSLERGTILSTKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMJ0</accession>
    <name>A0A8J4PMJ0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000761">
        <property type="protein sequence ID" value="KAF2069051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMJ0"/>
    <dbReference type="OrthoDB" id="20318at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016071">
        <property type="term" value="P:mRNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028322">
        <property type="entry name" value="PNRC-like_rgn"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15365">
        <property type="entry name" value="PNRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="131" mass="14980" checksum="89EC66A81C65F6C6" modified="2022-05-25" version="1">MSTVAYSEHNHKKSNKTQYFHQNHQQQIYNHQLAHHQHHQSPTKYKTTMSHTKNKFNTRMKSPPSQNINFWAGGAFTNSPSPNSLPLPNFDDELATLTTTTSHTPITLEQMSFDLRRLLNIPQSVLAGSPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMR4</accession>
    <name>A0A8J4PMR4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_007964</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PI3/PI4-kinase family. ATM subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000454">
        <property type="protein sequence ID" value="KAF2070722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="19302at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00892">
        <property type="entry name" value="PIKKc_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056802">
        <property type="entry name" value="ATR-like_M-HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057564">
        <property type="entry name" value="HEAT_ATR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003151">
        <property type="entry name" value="PIK-rel_kinase_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012993">
        <property type="entry name" value="UME"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF69">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02259">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23593">
        <property type="entry name" value="HEAT_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25030">
        <property type="entry name" value="M-HEAT_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08064">
        <property type="entry name" value="UME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00802">
        <property type="entry name" value="UME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAT" evidence="14">
        <location>
            <begin position="2014"/>
            <end position="2580"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="13">
        <location>
            <begin position="2685"/>
            <end position="2987"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="15">
        <location>
            <begin position="2985"/>
            <end position="3017"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="51"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="749"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="749"/>
            <end position="783"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2070722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="3017" mass="347125" checksum="C65410DF3F5A932B" modified="2022-05-25" version="1">METTATQQSHKEQLVSRLVSLIIDIQQLNELDYNSPNHPINLNKDSAIINNSLDNNNNNSNNNNNSEQDDGNNNNLILSPSTAATINENKLKQQQDRDIKMEDINDNTHNSKKGVMEISKLTFKQKNEYYISMIRSLLAELINVHLVINAEIQDVTEIVKLIDMVIVRVPNGQAALGDSPNICFYLFKLIPFLTYHPPLNNTQSPVTKEMEDHLLDNLIITMKSVLNLLCGDINVLNLFFKESIVLFRDTIGFIQFSFSLQQQLQSLPLQQQQTTPSVYPLLIQTFSDLSVWALPESTGTISSSYSKTFQASDILIHANHLNKNNNNSNNNNNQKEIEENLKNADEGVLVVAHSLESLLNLQKGILESIHSALESTSQFLYPHIADLWGILLDFTDLIQSINSLSLESKFSFQTTLVNLYETLLKESKIPLIYQNRIFQSLESILSSTFQYTNYSNNKMEQSLSMILDSIFSQLSSSTIQDFFQRFIILIVPILKKSKSKEFKVSICKAFLKMLKSNNDNQFESIYMMFDFFVDQDIQDILYQCFDYVLGKSIEYEERLDSLSPSAATASSSNSGEIRKDYFSFQLLEFIIQFSNRLHVSGILLDKEFVKSYSLVTTTFHSHNQNNTNNNNKRKQSTNHIDVLSSQSQEEFSMKTSINLDYLSRQIKDEETIQSFIVSITLVFKILKKYKNIVSISYLLDSFILDLNELYKYLIKCKELSLLELKIIKDTMSLIDMLLIEYVHLNHSSNNNNSNSNNNNDNNNNNKNSSSNNNRGNRTPSSSNKTVQIESLVDSIYTFSFLIWNHPSILSEPLHQMIIDEIKTYSFSIISWLPNQIYLNERKNIILSTLSIYQDKQLKSNISTLFGSDYYLTMIKLLPVFLSNINPSSSCHSHQSSSSSSTQQPPQQQTIESYNSFFQQIIISLNEIIENNQVTNIILDIIGVIGGSLYCLKEGFCHIKDLKNSVIDSNSNNNNDNIIFLNDNMVLHRIDYQIINSINPLLIDFNNIIQENFKYLTSLWIPFCSCESQILSDSNLHYNFQFTKVTNSNNSINNNEQNDNPLKIKPNLILLINNNNDSSNSNNNSNNSNNNNILFSKIYSKLPGVIEANVDESNNNNSGFHLHSDPIDDIRIKFFYLLFIFDRIVFHSSPLNSTEVESFLKHYIENVLISSSSELLRLTISKIITHMVKYNTFINTHLLQSSLSPIEQQYSNTTTATTMTTTTTSIYNSSQTNSSIINNLAEPNEFALTSLFMIIHPFVKNNRSDQITISIIDLLKHTSEAIINQHLYNQQLLQQQFNNNESLISDNNSNSNNNNNNNLESLYNNQYILITLCTLIDNLYKPYVKTIALESIEYIAKKMNQTSKQLITNMREHIDPRIIENLNGNSQSLVELSTQILDCNLEEYLISSLPNILPSLFFKKNVNILKELKNYIEIKTQSYEFIDSIFEHVFMNSPDRNYLTSTIAFIASYVDIDINTLLTHLPGSFINKLILNLGSNEQEKISKAKKALEYAFIFTSHDRQDLDCLEKYLTKEFLGLMNSFTMIMTNKSKPTQEKMVMMNSFQKLLEMMNYFVNQFRPKIMAFLKLALKTPLEEKACEIWYTFVTRIDLRSIGPILNQILFSVLPYVITFKDKALLIFDYLLVKNRDILSPYFHSIPFLPAEEPLKPYIEILQSVKEQYDLKTQVRQLVTLFTNENNEVKIMALNRLKSILYSYRMEFTNMIQGAEIIDPLINELVKGLVIGCRDPFLKVQVAFTECLGELGAIDPVRLDFSLRSVSMVEKTSRQEMASELIINYLAKFVLSPSNPTPQDRAGYAIQEILKVCGKEQFWQTLPVEVRDITYPYLNSEYDLSLISKKVVNHSPDSPFFYPKIPYRKWIISWITDLILKSKGVFEPIFTACRGIVKDDLKLCHYLLPYTVLNIIDNDNEKDLASIHNEILCVLKTDTEQNNDNGQMCTQMIFQLINCLNYWMDARKRLFTKKNTPTNSSSNTDANQKRKFSSEIVRIESFLSQIPEYSLATAFYRCKGLATSLMYMECFLRTEAMKHITPKVLGPQAKDLIKNQVINNNINMMQKIFYEMDDMDSLIGLSKIRTEDPTIDEKIMDLESTGHWSDALLHYSFASKQSPNESNYKLGVLKSMFNLGHYDTLLTIVDGFDQQNMTDSLSKRLNSFKIQAAWRLSDWDITEHAITSPYEQNFEVYLAKILLSLYKRQEKDFNSNLYNARAYISQFLSSASLDSYSRCYPYIVQLHILKEIEQGFHLNKTNIDSCLTLWEERFKVLQPSFKVRESVLSVCREVLDIYNLKSHAADYYLKISKYARHDGKFEIVQNSLLRSSDIQDNPIYILEKAKFLWKESKDQSFEAINLLDSALKNRIPQLANSDEKKQILAQTNLLLGVWKQKCAFTHHNEIANHYKEATTYEWERGHFYLGKYYDSLLVNLQRINPNSPTSQPKDPNSYIKYAKHILTSYGEALILGHKYIYQTLSRLLTLWLELGSIFPNFKNLDIYPENKRANELKLKKIKERMDQLESLIPASYFLIFLPQLASRICHKNQDTYEILEKIIVKVMVGFPQQANWIMSSHFSSSATFRRERAKTLFDKAKKSEFIKNNQIETLFLSANLIKICNHGEKNAAKGISYQLPKICPELSERKHFTSIIPIQNSLNPQLPLNGVANPNFQVFSSSLPIISSFGTNIETMNSLIAPKKITIKDDKGIQHIFLCKPKDDLRKDSRTMEFNTMVNKLLKKDPATRKRNLVIKTYGVVPLNEECGIIEWVPNTSSFRSILGSLYPKVNNNNFIRDQFSTAIPPMDRYNTLSDLYPPVFYKWFLNRFPEPSAWIDARNLYCKSIAVMSMVGASIGLGDRHAENILFDSSSGSCLHVDFNCLFWKGLTFNAPERVPFRLTRNMVDAMGVSGTNGIYRSVCEETLRLLRNNRDTLIRVLETFIYDPYIDWIQNQSKSIAKQQGEVENDKGLTSLRKINSTLQGIPEVGLQLSIEGQVNSLIKEATDPIFLANMYIGWSFYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMV0</accession>
    <name>A0A8J4PMV0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NUC153 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_008627</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000550">
        <property type="protein sequence ID" value="KAF2070058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMV0"/>
    <dbReference type="OrthoDB" id="431825at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039754">
        <property type="entry name" value="Esf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012580">
        <property type="entry name" value="NUC153"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056750">
        <property type="entry name" value="RRM_ESF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12202">
        <property type="entry name" value="ESF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12202:SF0">
        <property type="entry name" value="ESF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08159">
        <property type="entry name" value="NUC153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25121">
        <property type="entry name" value="RRM_ESF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ESF1 RRM" evidence="7">
        <location>
            <begin position="256"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="NUC153" evidence="6">
        <location>
            <begin position="764"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="63"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="471"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="528"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="801"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="74"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="146"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="160"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="177"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="226"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="474"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="558"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="649"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="801"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="823"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="855"/>
            <end position="866"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2070058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="866" mass="99321" checksum="8CAF38BB10BF1156" modified="2022-05-25" version="1">MSKSNNNGGGGKKGGNKNTGGVTAGGMITDPRFISAHFDPRHKMTRKNDQAVKLDSRFGSMFTSRFISNAPTDEYGRKIEKKVDKSLKNNYTLDNSKQSASTTTTTTPKDDKKSNKKEKASAPVADSRFTTSSKLWNDFDSKEKDIEEDDEYEMDQVEQQQKDSKKQPAKEKKEKVGAAAAKQQPKPKQKESSEEESQDEEEEEEEDDENVESIEYGGFEYNSDTESSDAEEYEGDEVDPDEVASEEEDVPRGDATRRFAVLNCDWDNVNSKSLFILLSSFVPKGGFIKNITVYPSDYGLKQMAIEKQMGPSKELWDTSAKKANPIKEADSTLTYDRTEDAESFDGKGFNLEKLRQYELSKLKYYYAVVECDSVATANNIYDEVEGTEIEDTANVLDLRFIPDEQEFTNTPRDSCDEMPTSEVPKFDFSTSILKGTTVDFTWDVDKNRRKLLTKNYSKEDAKEEDLRAYLADPESSDESMASDDDSDDQVVDKKDKKSSIRAKYRDLLLSGLQDEEKEDDIKVTFTSAFSDAKTIKDEDSDDEEDMTVSFEGQKVEDSSDEEEDDDDDEDSEEGSEDSGVEGSDESEYNLLNNQDDDQEPSWAKDLKSDEEEEDDVREMVINTDLKQVGKRLLKEKQQRESANVWSDYQNKKKDNKNQKRRDRRLEIQEKIKEQEEQAKKDLKKKKSGKLTDEEKKKQAELELLMMDDDGQKKKGFNKKLMEIQAKINTTDPLEKKKLEKKLKKKNIDIEQLQKEQDFKLDVNDERFPKIFTDPAFAIDPTAPQFKRHSGMVDILKEKQIRRKAEKEKEEKEIIERRKQYKANNNNENESNNSTNVSKSNLLAMAENIKRKAENLKNNNNLKKQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN97</accession>
    <name>A0A8J4PN97_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007626</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000418">
        <property type="protein sequence ID" value="KAF2071050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN97"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="108" mass="12508" checksum="0A3358CE23FC83CF" modified="2022-05-25" version="1">MFFSRIKDQWDYLRGTALTARQSDPIIKSSRNQCIRNTTKPLSLKEIYRIKLNLPDQIPLTHLQIERIFDSEVATRRSMASHFATISKIAYNKGGNTLSRALYPCLTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPJ3</accession>
    <name>A0A8J4PPJ3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DUF1682 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_006894</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000338">
        <property type="protein sequence ID" value="KAF2071787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPJ3"/>
    <dbReference type="OrthoDB" id="10039147at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032469">
        <property type="term" value="P:endoplasmic reticulum calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012879">
        <property type="entry name" value="CCDC47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12883">
        <property type="entry name" value="ADIPOCYTE-SPECIFIC PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12883:SF0">
        <property type="entry name" value="PAT COMPLEX SUBUNIT CCDC47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07946">
        <property type="entry name" value="CCDC47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035223392" description="DUF1682 family protein" evidence="7">
        <location>
            <begin position="24"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="344"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="344"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="386"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2071787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="412" mass="47655" checksum="427002D2AD65B243" modified="2022-05-25" version="1">MNKKIYFILIGILLLGLISVSLSQPTEFNEVEVEEDIEYEGTGVENKYQSLNQDGGSEAPQPQEPEIIATEVREETEEMKKKRELDALPFFKRQKHWYMEITFIVIIIFYVINYFIGEKTNRDLVKLWGRKFQPIFEQNFAKVGNRDVFTIIKINASTFTFICTGRENCNGAQVTLDLKKRHDLFSVLLDAFGFGSPDKVTIEVALTKDVMDPLIFAVVKQKALAKFKSDHEDVNLFCSKYNQATGLSSSFGVLCDTDCLPSLFLKNEVVSTLNANENLFESMYFTDHSLINTKYQKALQFIFKFPKVSDIDKLVLLTKMAVHFIDIVAKVELPKAHKQKAEKLREKAKESVVKQAHQDRQEELQKKKFEKMQKEKEMVAKMTPEQQRKYDEKEYKKQLKKKASGKYKVLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPL3</accession>
    <name>A0A8J4PPL3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008226</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000489">
        <property type="protein sequence ID" value="KAF2070461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPL3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="217" mass="24989" checksum="EAD8F7FB6D31944B" modified="2022-05-25" version="1">MYFLSQLDENELSYWYIPVNFLGAFFAILFVSTAIIKNHYNILKVKLLLIVACISIAPFIVAYLFVGSEFFFVGLVIVEGLFMLIPFVFLHNIRIERDVSDFYLPIPICFFFVTVFNSIAGFLINDMALIMCGLSGSIPSTILLITYLVMSIRYKTTRIILQEQIIQIIQGEFVFYAAIIPSHKTVKTNMNYPKYYVQPENTLEIQDNKKSINQLDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPY3</accession>
    <name>A0A8J4PPY3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pectin lyase-like family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_007982</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000457">
        <property type="protein sequence ID" value="KAF2070701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPY3"/>
    <dbReference type="OrthoDB" id="24297at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31318">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31318:SF2">
        <property type="entry name" value="PECTIN LYASE-LIKE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035171301" description="Pectin lyase-like family protein" evidence="3">
        <location>
            <begin position="23"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="478"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="506"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="522"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="557" mass="60624" checksum="919E14A07FA9C65D" modified="2022-05-25" version="1">MNSLRSFIFVLSFICFVNIVYSNSLTLIVSQTSNSTSATCGDTLDNACTSIPNAIQSYLNSNTNDNSTTSLTILLNDGTYNATKNSVTQTLSIPLTIGSYTNSSQYVFLVDFNMTLPLFGQKVTQSLPSLSISDVTFVNSYWIIASNTSMNVSIQDTVFKNVNSYIVGLVSLEYHGAQDNAPTFSLSNVVLNATNGVQSTNLFTITNYNVALDNVTAVNIKYVNTIFMFFDSQVVATGLFFNLNSVSYAPIASVSSSFSVDSSYFNQNYGGWAGVFKLNGENNQPFDVKFSNCTFIYNSAPNTGGALYISPNSGQVQILQSQFINNYVNTGNSIGGALYLTSNSNVSIDGCAFKGNTAYAAGGTIAIFNTNINISQSTFYDASSYEGGALYLFESTVTLNYNLFSANNANMYGADVSCRSSNLTVISEEVIQADNYVCPDETCLITSNQLFTCPSGTNNSSSDSPKKNNNNNNETIKIAIGVSCAIGGVIIIGFIVYLIRRHHHHHHHGGHHTESSSLIDNHHHHHHGGHHHHHDSGHHHHHHHHDSGHHHHHGNHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPZ4</accession>
    <name>A0A8J4PPZ4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006855</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000334">
        <property type="protein sequence ID" value="KAF2071838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPZ4"/>
    <dbReference type="OrthoDB" id="15323at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="189"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="218"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="431"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="480"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="524"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="566" mass="63843" checksum="E09C2977C4B3D4BE" modified="2022-05-25" version="1">MSEPSEIDFNNSSIININNTNVINNNNNNNNNNTNNNESTMSFSVKDHQNSFSPPPTVIPNISETIGNALPSISRNNINSSSGNLILDNNDSLASALQNSNVSSSSPYFSTKTSMNEIIHFAKAPSPSALFFRIVVSWLFGLVCQVVLMLFNPSHPMQFIWGVLCIGCGMTAAFAITWSSLLLKFKITFLITFATTIYWVGTPITWQILYVIDMYPPPIGVFTCIFPFEGLSLVLVVMLIPRLLRNQPGFRKKVITAFSSLIAPYLSFVSGLIYFRLFQFSTNIGRILLPPAYTVIMKIIQLGLDIICVRCAHSGSNLLIIIGRIIASYYSFAVLSFINNPVSVVSVVLSKLGLHIFMSLFMIFPQVETKMMSYLPQSFIGWKTKFEKGNQENNAISTTVDGANIVENPFDKMAMETDPQFTKIHIKGTNLWFSMFSDLLAIIMAVTVYTMGNYGPTKLHYTQMFPFRFDEQLFFNFLKYLGICFISLFIAYGLIWIILNYIIKTLELKRIVSLISVNWYPLNHFYVSCINFMMGYVIVVVAMMPDLFSIPGIDFWNVATKLKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQI5</accession>
    <name>A0A8J4PQI5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">40S ribosomal protein S15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_006388</name>
        <name evidence="4" type="ORF">CYY_009301</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000675">
        <property type="protein sequence ID" value="KAF2069376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AJWJ01000292">
        <property type="protein sequence ID" value="KAF2072310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQI5"/>
    <dbReference type="OrthoDB" id="10258210at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="entry name" value="30s Ribosomal Protein S19, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_uS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_uS19_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="RIBOSOMAL PROTEIN S19P FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="Ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2069376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2072310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="63" mass="6886" checksum="F20503C0F1F3240D" modified="2022-05-25" version="1" fragment="single">ILIVPEMIGSVVGVYNGKVFNQFEIKPEMIGHYTGEFSLSYKTVMHGRPGIGATGSSRFVPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSB6</accession>
    <name>A0A8J4PSB6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006600</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000312">
        <property type="protein sequence ID" value="KAF2072080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSB6"/>
    <dbReference type="OrthoDB" id="19701at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051916">
        <property type="entry name" value="GPI-anchor_lipid_remodeler"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14859">
        <property type="entry name" value="CALCOFLUOR WHITE HYPERSENSITIVE PROTEIN PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14859:SF17">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035220483" description="Endonuclease/exonuclease/phosphatase domain-containing protein" evidence="1">
        <location>
            <begin position="30"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="2">
        <location>
            <begin position="246"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="559" mass="63870" checksum="D224974BB85814E9" modified="2022-05-25" version="1">MINLNSKSLSSCIIFVVILLCCSFNPITCAPSKNMGIISSIKEISSNSTDLQKVLSDMKRSINRHRYYYLNAFESIPENEYKLNFPLFDFKVLEAKQHSSLLNLDGYNNYRGRVVVKGQNGQSYATFGDVTIGNTFELLLQVDVSAPYTYTFSTPSFSPSPLLYHTDCTYIKTKILKDHNGLMSISEFICFNQHPTTFKDKALDKNYFTYTSTSSAQQHQTAATTNPDDSPKKVTWVSLNHTISVMSYNLWNYNTPWRVRKELLADIIAKESPDIIAFQEARYSAWAGEYKFDQYPALYGQERNQVQHISQALSLRNQKYHFTYLPSMVYPNNNQLQYEGLAIFSKHPILDVSSIKLSRDNSDKEDEHQRSCLRVQTFFQPANLAINVFTSHFSLSSAGSQRNAYEVYEYMSQFQKPYVFTGDLNALPSSDAIRFLSANSVYGTAQIQGKKADLVDTWYYFKNNPLMNSKVVQQQQNNNNLNMNNNLNDILCVNSDNINGYTFPTLDGFPSKRIDYIFKSDDKILDVIDFKFLGSCSKDSIYPSDHSSIVTTFSINRLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PST1</accession>
    <name>A0A8J4PST1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006099</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000267">
        <property type="protein sequence ID" value="KAF2072576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PST1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2072576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="693" mass="80182" checksum="DD49E83C6A24CA13" modified="2022-05-25" version="1">MNNSSSRSINPDHLFFQIVRDKVLFSYLLECGRLDFCSLGYLCKIGRWDIFDQKIDRFLTDKYNSNSLIDFRYKYNGLLLFLIRNTSLERFKKVSKLFPLFHFKDKSKQRDLLKSCLKLSLSDPLQGLESFTYFIKQFDKQLDKKSLGPVFEDIALYGNIDAFQSIYNHLEKFKLDKYLGTIANWLKVALEKDRLEFCYYLIENVIKPWSGAQEGGVKPNISIAVSVDPPKEIVNLLGGTRVHLVKENPKTSDQNQDLKRAILKGEIETINTLYSQLENKKSIKVDLTSYANLDTLKFMYEKLNYLSVKETAVNSNVEYVKYLHGQGFKFSSDIFGSSLPVIEYILENVDPNQITLKNLNYLKSNDTKSIELLYKAFGARIMKLDFFFSFIHDDSVPIVEFYMKNGKFEITPYLLSQVILSPKVFKYLLSFIKVPVSIAQNIVKQYTDKKVFTMIESSHYEAVETFNEFTKSNILLHSVVGKDLPSLAMETLFGTKVSEPKMLNLLVKQYTIESPPCQTMQSILKTILYECIKHAKTTSIQIITKTLSYCSSKQHCTIDLTFNSLNFGIIDKSILLSIDYLSKFVIIGLIKQHENDPLFLRSMVGNIKDHQILLDVFYFAKGKKIITILNHFSKLNIQFNEEIFQQYIKANEKQHTYQSTKTKSVNIDSTLLKNPDYFINNNLYVADELKGYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSX1</accession>
    <name>A0A8J4PSX1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Choline transporter-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_006771</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Choline transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the CTL (choline transporter-like) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000327">
        <property type="protein sequence ID" value="KAF2071914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSX1"/>
    <dbReference type="OrthoDB" id="44736at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007603">
        <property type="entry name" value="Choline_transptr-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE (SLC FAMILY 44)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385:SF4">
        <property type="entry name" value="PROTEIN PNS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04515">
        <property type="entry name" value="Choline_transpo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="334"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="363"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="401"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="432"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="492"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="524"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="37"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="86"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2071914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="578" mass="65671" checksum="83EF14D218E9E4D8" modified="2022-05-25" version="1">MGIEDQSQHQLQQEDRVSSPITNPHPQNSFQSPTLHPYPVGQQPVPQQHLPYGYQQPPPQQQQQQPYQQPYQQPSYYSPNNGQHSPNYYQQQPNPQTYQGTGELPYSQPNYYNQPAQHQQMPQPPQQEASQYLLNNNNGGGNDNIQGSWNIQAEKFPQASPYQDKWFSLFFGLHIIGFLIILISSYAKPVLYYVDGNYNDYVNYNFLGNYYLTIGVGFVLAIIFVYVWSVIATRHPVQLITVTFISYLAVTGVLAIIFFIFISWVMGLLMLICTFFGVFFFIACRKRIQFTAALLKSAMDLISDYPTSFRMGVASMFFNFLWVTLWGAAFTRIFLVYEGGTLGFLVFYLVFSLYWVLNIIKNIVHTTISGLFASWYFMSGSVGMPRNPTLGAFKRSVTTSFGSIAFGSLLIAIISTLRYFASMMQNNRNVIVQIIGIILNFILGIMESILGFFNIYAFTQVAIYGKNYCTAAKDTFGMFKSRLGDTVINDNFVSVLISFSAFLGGLLGGIFGVIVCYDRVFGAFIGFFICFSFVLIALEVVYSGVVTIFVCYVMEPHILQQTKPEIYNIYQDAYHLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT72</accession>
    <name>A0A8J4PT72_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 18">Inosine-5'-monophosphate dehydrogenase</fullName>
            <shortName evidence="12">IMP dehydrogenase</shortName>
            <shortName evidence="12">IMPD</shortName>
            <shortName evidence="12">IMPDH</shortName>
            <ecNumber evidence="12 18">1.1.1.205</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">CYY_005282</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 12">Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12 18">
            <text>IMP + NAD(+) + H2O = XMP + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="1.1.1.205"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="12">Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12 18">Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12 17">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.205" evidence="12 18"/>
    <dbReference type="EMBL" id="AJWJ01000206">
        <property type="protein sequence ID" value="KAF2073403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT72"/>
    <dbReference type="OrthoDB" id="418595at2759"/>
    <dbReference type="UniPathway" id="UPA00601">
        <property type="reaction ID" value="UER00295"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003938">
        <property type="term" value="F:IMP dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006177">
        <property type="term" value="P:GMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04601">
        <property type="entry name" value="CBS_pair_IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00381">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000086">
        <property type="entry name" value="IMP dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01964">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005990">
        <property type="entry name" value="IMP_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015875">
        <property type="entry name" value="IMP_DH/GMP_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001093">
        <property type="entry name" value="IMP_DH_GMPRt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01302">
        <property type="entry name" value="IMP_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911:SF111">
        <property type="entry name" value="INOSINE-5'-MONOPHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911">
        <property type="entry name" value="INOSINE-5-MONOPHOSPHATE DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00478">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000130">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01240">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51412">
        <property type="entry name" value="Inosine monophosphate dehydrogenase (IMPDH)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00487">
        <property type="entry name" value="IMP_DH_GMP_RED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0129">CBS domain</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 12 18" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="3 12 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12 14" id="KW-0520">NAD</keyword>
    <keyword evidence="7 12 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 12 15" id="KW-0630">Potassium</keyword>
    <keyword evidence="5 12 18" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="19">
        <location>
            <begin position="127"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="19">
        <location>
            <begin position="190"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="active site" description="Thioimidate intermediate" evidence="12 13">
        <location>
            <position position="341"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12 13">
        <location>
            <position position="437"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12 14">
        <location>
            <begin position="284"/>
            <end position="286"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 14">
        <location>
            <begin position="334"/>
            <end position="336"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12 15">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12 15">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12 15">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="374"/>
            <end position="376"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="397"/>
            <end position="398"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="421"/>
            <end position="425"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="449"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAF2073403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="522" mass="56682" checksum="F9695BB93B2E8A05" modified="2022-05-25" version="1">MDALHNIGMSNKKTTLVGGKQIEPSMIVDGFDVFELFAQKYGYTYDDIIMLPGHINFSADDVKLGSKLTKNITLNVPLVSSPMDTVTEHLMAINMALLGGIGIIHYNNTIEEQVAEVKKVKRFKNGFITDPIVLGPNNKLSDVDTIKAKYGFSGIPITDTGKIGGKLIGIVTSRDTDFIKDRSTALNEVMTTDLYTGHANCTLEEANSIMKKCKKGKLPIINDNGELIALASRDDLLKNRDFPNATKDKDNKKLLVGAALGTRETDKLRLAALSDAGVDVVILDSSQGDSVYQRDMIHFIKRNYPKIDVIGGNVVTCNQCESLIQAGVDGLRVGMGVGSICTTQEVMAVGRPQATAVFKTGIYSAQYDVPIIADGGIRTIGHIIKALSLGASSVMMGSMLAGTEEAPGDYFYKDGMRLKKYRGMGSLEAMLKGGDQRYFSETDKIKVAQGVSGSVVDKGSVRKFVPYLIQGMKHGLQDLGCDSLSKLKESVYNGNVRFEVRTTAAQVEGSVHSLFSYEKHFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTB3</accession>
    <name>A0A8J4PTB3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Prokaryotic-type class I peptide chain release factors domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_005758</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000238">
        <property type="protein sequence ID" value="KAF2072936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTB3"/>
    <dbReference type="OrthoDB" id="2019491at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000004">
        <property type="entry name" value="Peptide chain release factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1660:FF:000002">
        <property type="entry name" value="Peptide chain release factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050057">
        <property type="entry name" value="Prokaryotic/Mito_RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004373">
        <property type="entry name" value="RF-1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00019">
        <property type="entry name" value="prfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001859">
        <property type="entry name" value="PRK00591.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43804">
        <property type="entry name" value="LD18447P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43804:SF7">
        <property type="entry name" value="LD18447P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="Release factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prokaryotic-type class I peptide chain release factors" evidence="4">
        <location>
            <begin position="327"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2072936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="459" mass="52672" checksum="B1A848E3877C6CD4" modified="2022-05-25" version="1">MIHFNTSYLKENHNFRINPALPKYAQSFIIYEQALEKPNKYQDLDSNHHVAAALDKGFYNSSHNSINKYIYSNSSHCLFTRYYTTEYNKKELFHKSTVPLKIIDRLETKTSKLKELRNYLDSNQATLQQNEIKRITKEINESAESVDYFASLKDKLNERMELNEMYSMETDAESKSMIGEDIVNLNQQVEQLEKSLLFSLLPSNKDDNGNAILEIRAGTGGSEAQLFALDIFNMYRGYAENKGWEFDPMDMSYTEVGGCRDASASISGRGVFGFLKYESGVHRVQRVPDTETQGRVHTSTITVAILPEPKEVDINIKDNDLRIDVYRSSGNGGQSVNTTDSAVRITHIPTGTVVCMQDERSQIQNRTKAMKVLRARLYEAERVRLQTERDTDRNSQIGSAARSERIRTYNYPQDRVTDHRVNVTSKTLHTIITGEDLDTFIEEILVNFQTKELEELKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTF9</accession>
    <name>A0A8J4PTF9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_005672</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000232">
        <property type="protein sequence ID" value="KAF2073026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTF9"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003492">
        <property type="entry name" value="AGAP012735-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF172">
        <property type="entry name" value="ABC TRANSPORTER C FAMILY MEMBER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="48"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2073026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="164" mass="18410" checksum="D58517A7E7B61803" modified="2022-05-25" version="1" fragment="single">SVSFPKIHSFSLAQLEKTSIHLKNTPIKKSGKHWKRPISKKQSNTCLKNSTPVLEGGDGLSVGQKQLLCLSRALLRNAKIVLMDEATASLDYHTDYIIKQTIKMNFQNSTVLTIAHRLDTIIDSDKILVIDQGQLVEFDSPQNLINNPTSRFSQIVNAQTTFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTY6</accession>
    <name>A0A8J4PTY6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Myotubularin phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_005119</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000196">
        <property type="protein sequence ID" value="KAF2073570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="271628at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14507">
        <property type="entry name" value="PTP-MTM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF80">
        <property type="entry name" value="MYOTUBULARIN PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myotubularin phosphatase" evidence="5">
        <location>
            <begin position="1148"/>
            <end position="1521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="314"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="391"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="815"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="13"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="50"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="314"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="351"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="398"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="824"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="2">
        <location>
            <position position="1359"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="1298"/>
            <end position="1299"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="1359"/>
            <end position="1365"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2073570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1556" mass="176916" checksum="FEBD94F3675E77E8" modified="2022-05-25" version="1">MGLQSILNDKDTLSTSSPVQSALSVNENTTECIDKERDPLASLETTPITPTTPTPIIPITPTTPTPTTTTTTTPPPILKKTESSNTANSNNNNTTPIINANQNRPEDEFYKLYLDYQKIHYHNSNKFGGILDFNTKINYSNTGYNNNSNSNSNNNSNSNSLSSLLSTSLNDQQSNASIELTIMIHISPWLNEDPQELTLKDQRAINIGDINVFFKEKGTFIKIKNLHNYYLYKNLSNSTVKKKLLMSNDQPLSTYNIKNYDDLYLLQVKSNSNNSNNSSNNSSNTSTNPLPRWFYKVIVPSIQLEGILKELNSKTNNEKSNNNNNNLLHFSTPPPHYHRRHNSKDDPFQILSSSANSNSNTTSTSLSKKNNSGGTSAYDIIENIKSKKLVTIDSPSRNNNNTTTNNNNTNNGLPIKTTQQQWSYATPSSVPPHHAWLHARRVFDVVNKIESEFPYISDPRLDSIEEDKWEARINSSDDSFIKTLSMEDSLGSELIKQEYIEFSLRHENRSALLIGIEYSFVVWIDPIQSFFTLSFSSNTTVRDAVYFILQYLFPSNNSNNNNKSNSKSSITNKDNDDIDMDMETFLLMLPHYGLYLQDSSTVKLIPLQHKQLLSNQRSSHQSMLVFKRKKYRVNTNDLNNYRKKRQSFHTVSNLSISGEIVTSNNGQSSSSPRPSIEEFEDEGYNLTVLFPTKQTYRQFHFIPTHTVNETIQMIVSAIAPPPSLRFGNNNNNSSGSAEDFALNDEDSQHLSEFEHLGGAPTYQVDNNQPLFQPQSTVSLPSSPIALSRKNHLEFRNVQLNSYSPAIHSPILSTLNHHVNNQDPSSSNSEDGQQQQQLSSSSSCSSLSSSSFSMPISGGNGMMSTRNYIFETNEETSGFCLYFQKSGSGVWMEGNRTLISYNLPNETVVEFKPPPTISKLSLIEGVNHTIKLLCKSLIYTLVYCKFTQILSLEDLISKKISSLSKKEAESFGLFLVRDEPNGSHLEICLSSYPTFKDLDIEILPTDILQFKESTATTIDSVAALTFGAPKDPPLISGETIVMKRDGQRLVSDQLKKGVFILTNYRLMFNALNRSSYVDLTKEDCDIPLSSILKVKNTNGNTEIQCKDFRVCTFNCNDSNLWHNLKSMVDTNSIQKTFAFKNLEQFDKKYDTIYNLWDEFERLQFPLDGWRVTHVNEDYSLCPTYPSKFIVPKNILDTDLKKISMFREKGRVPAICWIHKNHKSTITRCSQPRVGLRGARCAEDEEYLKSITESNSNSTVLYVMDSRPMANAKANTLLGKGHENTALYQNVELQFLGIGNIHVMRDSFKKLYSLSQNLEASNWLSILDDTAWLEHIYQLINSAHMVVELVDKKGTSVLTHCSDGWDRTSQLVSLSQLLLDPYYRTIKGFQILIEKEWLSFGHLFATRCNHINSLNEDDFSPIFLLFIDAVWQLTCLLPTTFEFNETFLIKILDSVYNCRYKTFLFNSEKEKNQNLKSHPNLVSLWCAINSNIEPYVNCFYIMNSKPIFENFFIGDIQFWSNYYLRWNEKYRPKLSINSSILQEIDRKKKKQPLSSPSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU07</accession>
    <name>A0A8J4PU07_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009209</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000655">
        <property type="protein sequence ID" value="KAF2069476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU07"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF180">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="520"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="523"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="557" mass="63584" checksum="C585179AFC0F5F8E" modified="2022-05-25" version="1">MTKSFYLIWRNIYIRRLIRNHVCQDLVFDVLVDGWQKLDDIDYKYLSLFTDKEKLDYNISIRLKGRDVVEQYVDNQFYDLINDVDIKYYVEDILDFSVFHRSVQKLSLTIAHRSSTAINQLPSSLTYLYVDCPRHSPQVLKHILSSLPTNLKTLRLQETLDENMITSECRLPDSLTNLDFSSVYNNFKWFVVPPNKVFKHSILVVDSMDAITWLKSNKWIQTIFVVSTGKYIEVSSANPIPEHAINVTINSNVDLIEKGIVFSPQLELLKCDYGIPFSHYTHLRALILNGYPEKLEMHVLPITTQLLQISSYNQPLEANVLPPNLTKLTLDNFNQPLSQGILPNSLTELSLREFDHPLMPFVLPPNLTELNIANFKQQATISPNTLPSSLTYLDMGSFTGSFEPLCQPLDHLTDLLISSLDPTVSTILRNVKDLDLSIETIPNARYGTCLFNTGIETLYLQIPNTCSLYPKSFPPTIKNLTLDNFKIQSKGVIPSTCKYLKSLSMLIYPDFIPKSTIYKKIDDDSDSDDSDDDDSDDSGGDSDDFDEDSDYTDDDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU78</accession>
    <name>A0A8J4PU78_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AP complex mu/sigma subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_004695</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000171">
        <property type="protein sequence ID" value="KAF2073988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU78"/>
    <dbReference type="OrthoDB" id="10249988at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039652">
        <property type="entry name" value="Coatomer_zeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11043:SF1">
        <property type="entry name" value="TSET COMPLEX MEMBER TSTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11043">
        <property type="entry name" value="ZETA-COAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="6">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2073988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="154" mass="17851" checksum="40FEDC8CE3E71DF1" modified="2022-05-25" version="1">MINTILITNWNGNILFSKYYNSITEEKQIEFEKLLYQFTKDEWLNSKGEKHLVTEFGGSVTVYTNVGDLLLFVSGSDEYDELALSDILIPITDSIKDMCKKKGVTETHYIEQIPKFVLYLDEIIQRGQLDQVQFEIVSNYSSLKHDQPLKKESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUM5</accession>
    <name>A0A8J4PUM5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">phospholipase D</fullName>
            <ecNumber evidence="1">3.1.4.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_004406</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.4" evidence="1"/>
    <dbReference type="EMBL" id="AJWJ01000155">
        <property type="protein sequence ID" value="KAF2074273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUM5"/>
    <dbReference type="OrthoDB" id="14911at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004630">
        <property type="term" value="F:phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09138">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09141">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896:SF182">
        <property type="entry name" value="PHOSPHOLIPASE D A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00614">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="8">
        <location>
            <begin position="327"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="8">
        <location>
            <begin position="597"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="698"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="857"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="44"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="698"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="716"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="782"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="814"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="862"/>
            <end position="883"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2074273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1018" mass="114530" checksum="F836215514AA61B8" modified="2022-05-25" version="1">MNDRQGLSSSGSKRNTSGSGGGSSGKQQSYRQFPHHYQYSTKALQDFEDKRKLLIEQLKSVKINLDSQSEDDPISYLKIRMELNKVKASLESELKTLDEVLNTTTKIEEPISTPIDGITSLLNMILPAGTTTTTTTTTATATATTTNTIVANIQTIASTTTTANQQRISPSTTSTFRSSNLPVSCVPQPNTYNYPNSIIPTSTPLDNPDPNHPRFPSRDNINVKFYVDCDEYFAASAEAIENAQREVFITSWFLSPEVYLIRYPNLDPRYRLDNLLKRKAMQGVKIFVILWDETKIATFKGSKRAKDKLEELHQNIKVIKHPPIIPLYWSHHQKTLIVDQELAFVGGVDFCFGRYDTWCHHLIDVNSNLWPGKDYYNPVLGEMGDILAPNEDSIDRKKIPRMPWHDVMVGVNGLAARDVALNFILRWNHHKDDYYPPLYFNTTPLISQGTSTCQLLRSMDEWSGGGRNERSIHTAYIQAIEDADHYIYIENQNFVSTHAPGVWNQISHEIVKRIKRAIRKREVFRVFIVLPSQQDGKFQEETHIRGLMHWQYATLIRGENSIVRQLSREFPDVDLTEYISFFSLRTHAFLEGSYVTEQIYVHSKLMVIDDRVVIVGSANINDRSFNGERDSELAFIVRDDKDTIKTKMNGKEYIASRFAFNLRIRLWKEHLGLLPPIDHPPLFNNNINGNCSGSSNNATLLVNNNNNNDKPASRKNSYNESDNENVNSNSSKSLESSNVNNNSNNKLSTSISSSSISIPNNRKPITSHHRSSSFQGIVVQPPFSTRSNQPTPTEQSPLDSPRTESSETLNNDNSSTGSTGSGSLIIEDYQNVSGSCNSFENLPKLSLQPQHNLSVTQLPSINSSNNNNNSGVNNNNNNNNVPVSAVNEDNVKVVVVSDAESPRTQPRSISSLHDYSDDSYCPQSSIDLIDPICSDFYFGVWIGTAASNTRIYDNVFPAIPKDAIKTVDQFNQLSKLPVSLADSKLLSEVRGNLVFHSLHFLSDEDIQPTFLFTDDLFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUP2</accession>
    <name>A0A8J4PUP2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vacuolar protein-sorting-associated protein 25</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">ESCRT-II complex subunit VPS25</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_005687</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000233">
        <property type="protein sequence ID" value="KAF2073009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUP2"/>
    <dbReference type="OrthoDB" id="245150at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000814">
        <property type="term" value="C:ESCRT II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000141">
        <property type="entry name" value="vacuolar protein-sorting-associated protein 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.570:FF:000003">
        <property type="entry name" value="Vacuolar protein-sorting-associated protein 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.570">
        <property type="entry name" value="Winged helix' DNA-binding domain. Chain C. Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008570">
        <property type="entry name" value="ESCRT-II_cplx_Vps25-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014041">
        <property type="entry name" value="ESCRT-II_cplx_Vps25-sub_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13149">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13149:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN-SORTING-ASSOCIATED PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05871">
        <property type="entry name" value="ESCRT-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2073009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="187" mass="21930" checksum="DC1C0E8E0590BB6D" modified="2022-05-25" version="1">MTNKEATAFQFPVYYNKEPFFTVQPVLDTRKKQFQMWRDLILQYCSSLKIYELDLLESIKSNSILFFNQKINRRLSIESLKLIIQDIVDNGYAEWLDKDKSRVLILWRKPEEWASLLYKWVSDCGLANSILTVWEIQNGDDSKSQEFYQLNTAVLMKSLKALEKQNKAQIFSSNGDDSNLGVKFFSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUZ8</accession>
    <name>A0A8J4PUZ8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_004540</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000163">
        <property type="protein sequence ID" value="KAF2074137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUZ8"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF184">
        <property type="entry name" value="GTP-BINDING PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="4">
        <location>
            <begin position="5"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="204"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2074137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="342" mass="38630" checksum="80053D8DF34DEB9B" modified="2022-05-25" version="1">MEERTVLVIGKTGNGKSTICNVISGSNDFQEGEFGVSQTKDHLKKEYIVDGVKYIIVDTIGIGDTKLSLQQVLYKIADACYSVKEGLNQVIFVTTGKFSEEEQMSYDLLINVIFSLEIVKYTTIVRTRFGKFRDPHACQNDINLISSENQGLAQIIKGCNKVLHINNINQDDDVDISIKSRNDSRIRLLTHLYGCKSFYKPKELDQLNARIAGHMTDVQKAKAKLVELNKVLSETSKTNQELYKNILNDKKQYEKSLLDLSKSNQEKEENISKIAELTRAMEEKERTNQALLSKLESEKKAYISSIEDAKLKVSQATQGVLKEKSPGVWEYLSGVKKKCSIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVS9</accession>
    <name>A0A8J4PVS9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004870</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000181">
        <property type="protein sequence ID" value="KAF2073812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVS9"/>
    <dbReference type="OrthoDB" id="16283at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.10">
        <property type="entry name" value="endopeptidase domain like (from Nostoc punctiforme)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="396" mass="45223" checksum="F6891D5BF8024865" modified="2022-05-25" version="1">MGNKHSIASESKIENNVFTLYVMNCDNTNFFPENKAKNVAKYGDNKLTVTMEYNGARKSISTRFVKRAIWERGVFQFDLDSSFSKDFTLSLSTDASGLKWLDKPLSMDDLSENTIHQIVFRSNEIENELDGASASAPSADSVTLNIFYTTKLFDIYYDDSNNSNNNNTLDIDSKLLLNTRWTNSQKRSLIFRENIDQLEDGDLILYCGESGMSDLIRDRFYRPWSHVGIVVKAYPFHKKGEPRSTEKETYVLESVGKENIKDPFRTRQSLTGINLFKLSDRIVEYEGYCVQALRHPTPLSTEQRERLLTAAFEVHGSRAPFDVFQAGILMLERLNLWNIQSNYAVFCSELVAIMLFRCGLLPDELYKPANTDPGECSDFPCFGGVHNSPMFLKYDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVY2</accession>
    <name>A0A8J4PVY2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005677</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000233">
        <property type="protein sequence ID" value="KAF2072999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVY2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2072999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="766" mass="89207" checksum="0ACE6EA7CFF96F8D" modified="2022-05-25" version="1">MDALYQSVFRNTFLYRYILNHVKQETRYDEHVTLEEYRHTHRSARQYYSFTDLTIGVITKSIKNKKSVLREKLRCFFGLYLTDPIKYVEYCLSITSNADLVNLVSSGAVQSVEEMQLLYRQFPKEFQQYLLLNASCAWACGSVAVLEYLQNELECKVCPKTMGLTDFFKLIGTGGSVAVLEYFIQTFVIKHPKTTIDIEVLFSFYSDNKLHDFYLPVLKIVSLKNWTNINILMKVSVEKDDIVLAQQLVEQLKTRQSQLESTPLITLFHYDKGSIRVFNYYLESGIFSEHYYRLKYKLNFIAMQDNRQELMVFLEKYSSLVEPEHYYALSSVKDYQTFKLLESTIKPLPLSPQLLCYAATELRDLEFVKYLIETAGVRYSYECMDSTFEIISYFVSKGLLFDDFCVKRAYHGDLKCAEYLHDLSESHTSLVKMSSHSLNYAAQQGYKELVRYLIQFYRTDITTVKSVIDKDDAEVFQILYESIDDARELIYDPNISQICNSKPNIAAYFFLTRNFRDIPFPQDLHLLGTLHAMNKVDSVSLKKMLIKALDSNDKKRCRAIIETFNVQLSTRITIDEYLHHSMEVDLKHLYYHLVVSNNSLLFYLLSQDKQLFLVDSVSLKTILSNACLNQKLDVIKFLILYASACSKSTDQPTSTTTAYQEIYKTIVFLSIFYSVSYYEKDKLKHRGPNLILEYLVNDSFQLEYPLECAELVVKNKLIKVELHAQTLDKLFLTAMANFSPEQINILNNTYGITKEIITTYCPELNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW46</accession>
    <name>A0A8J4PW46_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003879</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000128">
        <property type="protein sequence ID" value="KAF2074815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW46"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="222" mass="26057" checksum="8D6ABE3EDDFAB179" modified="2022-05-25" version="1">MFSWFSKSKKEETPATPPPSTEKVDEEEKEVEYDPKLLKELETKDPRSYKLIKGHIKKEETRKEQYRLINFILDPPTNHMNLTVEKIDKAINDDDLWRVRFRMWECGVFGGIPTFALASYLTRNIPFKKTQTCLILGATTLGAYFPANAYYKSHRKEIAEYDETWYQLLAERFFLEERDKVLKESQKQYTRDTESIHDDFNSNSNSNNSNNSSSSNNDKAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXE7</accession>
    <name>A0A8J4PXE7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000117">
        <property type="protein sequence ID" value="KAF2075050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXE7"/>
    <dbReference type="OrthoDB" id="18208at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="780" mass="89575" checksum="DDE4D443E209E1D0" modified="2022-05-25" version="1">MDSMASMKIIKKEDCEGKIKLDESLFKYNVPPTVSSSSSSSSSSSSSSTATAIATNTKTTATTASTEATTIAVITEESIEKKEKKEKTNKEKETKDKDKEKEKDKDKDKDNHSNNNKDTSKPNIPEQSQETIAILKSLSRTLSSTNVDEKEFHGTLPIYVIKEILLLCHDDKFIPYSWKVSLGLVSKTFFKLLSSIMIDVTLTSKGLYNHLRNPACLFKNIKTIQASFSNLEGYLDEFHFEQLEKGLYVPIEQSSKSTPIKSPLVDLTSITIRGQDTYVKKEKTALQLLISRSLDSLEHFSLISHKTTLIKNIFGLLDVCPNFKSFGLGVPHAEKSYSPMSKFIKDKAETLERLQLCFYFKTPLPYNSDQSQFHTTLLSDEFIEKQASETSNHLKSLTLGHKKMVGSDYDPHQFTYPLISSKVLFEQIGKLTHLSRLIFCKQSIDSNPEFYQHFYTYFESNETITHLELPSKINDLALVQLLAKKNNLKRLALSTSVMVKFSQGLQHLELFGCPVHILNEFFESNKESNIRVFKTTEYLPFDGMSQFLVNNQKIEKIKFGLKGKTVNFLSLTDNHSLKKISIKFNTPDKNEVHEPINRLLKACSLNDSIQYLKISSNDISMDKLIPIDFNYHNKIDSFHYFSRSFPKIKKNVPRLNTSLDSWVGIEDKYETMKKLVLEAKQYYEELNESCKNIKDFNYNACYLLDYTKQNLDKYDPDQWSQSDVRLKLAYNTIEALDKQDKFIDEILTQYDMNIDFVKKSVDKIFNGKEVMPNKKYKKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXN1</accession>
    <name>A0A8J4PXN1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003448</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000108">
        <property type="protein sequence ID" value="KAF2075230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXN1"/>
    <dbReference type="OrthoDB" id="20631at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035220468" evidence="1">
        <location>
            <begin position="20"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="490" mass="55600" checksum="5596D9E3068EF620" modified="2022-05-25" version="1">MNFIYLYIFLFIGILFCFGESKQQQLDFLTHFHSDEINQPLIQNILYHIESFSFVNYFSKINLNNNNNKNDVDYYQGYYATVGSKKLANDISLLKYHIQNRIPLILIDVDQADKQHLCAMVNGCFNGTNNILAYIPRETKDGLKFHRIYNWMAAVVDQQRNQDDQTSDVNFTPFNHFTGEFLEKLVGDSSPISALIPPSNTAIPFSYYETSVSGSFNLQAKGKTYVGPVQTFSNRIDNHVYLYKDVPDNLIYFGLLQNSYAFPGSPIGINDPTRAIGYFQTGFSIANSPSYNGAPLDQRYFNLEQTSPATVNQAHQLTNTVSTSFSVEVSFSIGPDGPSGGAGTKAEWSSTTTDVENISDWGINEMTNPSTMKTQWMFHQQFPYDPETLGYANFGSWWQQAYSNENVKYPPSLSTNTLQATTLSVWKASTLLVDPSTQMIVMDIDYSIYHAACMVVHKDTFYDSHHKLFYSLTNLNEGRQSVNLNYFPNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PY20</accession>
    <name>A0A8J4PY20_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Selenoprotein F</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_007145</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the selenoprotein M/F family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000364">
        <property type="protein sequence ID" value="KAF2071546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PY20"/>
    <dbReference type="OrthoDB" id="19100at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.50">
        <property type="entry name" value="Sep15/SelM thioredoxin-like domain, active-site redox motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038219">
        <property type="entry name" value="Sep15/SelM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039992">
        <property type="entry name" value="Sep15_SelM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014912">
        <property type="entry name" value="Sep15_SelM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13077">
        <property type="entry name" value="SELENOPROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13077:SF6">
        <property type="entry name" value="SELENOPROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08806">
        <property type="entry name" value="Sep15_SelM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0712">Selenocysteine</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="Selenoprotein F/M" evidence="8">
        <location>
            <begin position="80"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2071546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="149" mass="16878" checksum="FB248DB6832B39E9" modified="2022-05-25" version="1">MNKIKNRGHLGHRVESAFNLSVVVLLFMFIGGSISVNIPQNSALQSTCHELGFTDALLCSTCDDFKEFVGDSDFYGECQKCCAEESNNEKKTFEFIEKKAADYKKLSVKYQSGSNPRLSLTDTNGKKEEINIESWKTENLEEYLKEQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PY41</accession>
    <name>A0A8J4PY41_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002655</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000075">
        <property type="protein sequence ID" value="KAF2076041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PY41"/>
    <dbReference type="OrthoDB" id="343296at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000001">
        <property type="entry name" value="Calmodulin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050145">
        <property type="entry name" value="Centrin_CML-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23050">
        <property type="entry name" value="CALCIUM BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3" id="KW-0518">Myosin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="84"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="120"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2076041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="155" mass="17682" checksum="FFE60C0564EF1870" modified="2022-05-25" version="1">MAKSPKQQKQQTKTPQNLTDDQLTEIKEAFDLFKSDNAKLEITQLKYACRALGIELSQKEKDLLKFYTLAQFTDLVSSLIPKRDSPSTLEQAFKLFDKDESGKISFEDLKLVAVNLGEECTDRDLQEMIEFADLDGDGQISKHEFMELISGKRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYQ7</accession>
    <name>A0A8J4PYQ7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003664</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000118">
        <property type="protein sequence ID" value="KAF2075012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYQ7"/>
    <dbReference type="OrthoDB" id="29094at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44207:SF2">
        <property type="entry name" value="REPEAT PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44207">
        <property type="entry name" value="SURFACE ANTIGEN BSPA-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="98"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="131"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="164"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="197"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="28"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="290" mass="31479" checksum="65E1AB7315D223BB" modified="2022-05-25" version="1">MSNPAGSSSVSLAVKPMNNELLHPSEAQLGYSRGDSNNNCNLNNNNNCNQSKQQQQQRRIIESFQVMDIIIQLFLGKDYQSIDNILNSGININSQNAEGTTLTHLAVSTGNLQYLQYLIFKGANINISDKLQVSPLHSAVANGNFDIITTLVQFGANINAVDEEGDSPSHYAIRESKTQILSFLLTKGANVNAANDDGESLLHLAACLGEYECAKLLVSAGANTKVVDECGVTPIEEAKANNNFDLAQLLSDSKMSAADSEHQYPFRDFRQVQFGSNTPLCAATASSYYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZD5</accession>
    <name>A0A8J4PZD5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_002920</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000086">
        <property type="protein sequence ID" value="KAF2075787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZD5"/>
    <dbReference type="OrthoDB" id="19174at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22411">
        <property type="entry name" value="KH-I_Vigilin_rpt8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051616">
        <property type="entry name" value="Cul2-RING_E3_ligase_SR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46224">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="69"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="102"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="164"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="243"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="319"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="322"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="338"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2075787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="365" mass="40283" checksum="65BB8D1AF118DE8E" modified="2022-05-25" version="1">MVDDMTTTTTTTTTTTPMSDANEKEQFEIQEQQLLNGGVMKTAAEQGDFDLLKLYIEAKSISPDQQDMFGDTALHGAVAKGRLSIVEYLLKKKANPNILNKVGSTPLHKSVTIQQGIIPAHQHYQIIEILLKNGADPTIKNMAGLIPEQMTNTTRIKEILQGDEAVTIQVSVVKTSHGKVIGKGGKNLKDLRELTNTQITVPEPKTTNKNITIKGRKEDVEKARAMILELVTPKEEEITEEEKKEQEEIEVLNCKSIPKEKHKLIIGTGGKTIKHLRNEFNVKIEIPQSDNPDSTISITGKVPDIMAAMKYINESVLKKPLNNNQNNNNNNNNNGNFRGNGNGNGNGNGNRERKEKSVSSAVKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZJ1</accession>
    <name>A0A8J4PZJ1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001587</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000039">
        <property type="protein sequence ID" value="KAF2077078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="124582at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33099:SF13">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33099">
        <property type="entry name" value="FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="359"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="449"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1673"/>
            <end position="1693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1709"/>
            <end position="1886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="359"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="375"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="454"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1676"/>
            <end position="1693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1717"/>
            <end position="1729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1738"/>
            <end position="1749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1766"/>
            <end position="1785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1786"/>
            <end position="1801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1820"/>
            <end position="1835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1844"/>
            <end position="1856"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1886" mass="217097" checksum="3AEC1F4679FA8BF9" modified="2022-05-25" version="1">MDNVELSHTNTINNNDKNNNNNDNDNIDHIKGIEQKLRENDEYKVLTNTSHYLKESIEVPISLKVVDKFLSFPCIESQLKFIIDKYATKDQKKIVDLTTAKTNIWQINLNKIQVGGKTWPNTIDSILNRVKEVLGIACDEKIEVELQKFLILGQGSFIDNNNNSSSSSSSKNNDKVIANLFLILPSEHTGGEVSIEYSNSHYTTLNLENNTSSEIVYLGFYSDCKYQISPINSGYRVCLLYNLKKTGKRTLNSNDLTSMNQSSVAHLKYLNSLANQLLLQLSKKDNSKLIYILNSNNSRSDKDNSHFSSYRNRNLPKLIQSMNLSEPDLKISQILFDLNRNRDYNLSVFLTNIEISEYDSDGDDDDDDDDDGNDNDNNSNSSNDSNNNSINNNNNSKHFSAKLISFIDLDDLEFDIEFDNINDFQEISISQSYLLNSIQLDSSNNKTKSKEIESTLNSNPTTTTTSTQATPLSKSSTTILSTKKIYGIVLFKRSEYENIIGKESTSSSIGVLALLLKNQIQNISVRSNTTDIIDVADLILDSMGSKCNPFDPLNKNIEAIINYFCPTQKDYIEKENKRRLLKYPKDFIYHLSKYLDLFTSSEINETILSYNKDQNNINIKESIEILENILIPFYKKHKIITKSTITTFICQFQDLILPLYKLNNNNVIQPVDQANDFSSKWKLLDEYNSTFESYIKKQMKSNSELLISPNIIIYNQMLKFFDCLPEKLLNEKKEFVENYLQKSVIKYYQDSTLSSKQLYECWISLIEFGKEEQMNLAKFITDHYKYNIIWPLILLFRKDVCSKSTFNTLAYSFLLKSLLAILIDSKENSIPPVQLHAIWIYLFNLGGSEKQRQMDFSQYIVNNYQFDKIWELLLCFKNEMLVKASFNYDAYLFLWKSLATLAINSLSALFTSQQLYYIWINQKDSDFQKEFSLSILKKYSQDVVLEFLGNFKLSTKSSKFFNLLNFISIWKDFIQMVYLDFQTSSCQPIQVKNYWKKLLIGDDWSNVEELSSLSIELQSKLVSSSLGLYKDKPLELSNLFIEIDKTMIGNESFDYKLFLNIWQQYIDTLVKEKYSALSTSNIYKTWAQLLFLKNSLEQQGVFINFIINGYSFPQIKDLIKKIKKDITDSDQFSQKSFIALWTKAIGFMESSISTLLPQDYYDVWISLLDNLEQLQRPCQQRLIQIILDSPKVQDILILFKNEISANQLFNPRVWKSFLSLLLLMKKQTFSPQQIYQIWINLFAIEPISTTLQLQMDLVVDHITKLYSKESVFSLLLMFDKTILANPVFNQEAFVSLWKFYIKNVSATDVCLTPTQIYDSWSILLNLDKERDEIQMTFAKFIATTYSLEKIREIIVKFRVALKSSTLFHQNSLNYLLKFYLDCIVKSKSCLENQQILDLWTTMLDVKDDSKDYQAKLISLISIYSIEKRKDILSLNFEAIRNHLSFNLNVFYTFFKSFIEDLKNVKPSIVLTVQQIYSFYFKMVELKFDLPDSNEIQLLFANYIISSYGRSVVYQLSQTFKFDIFSHSHFSMNAFITLWKSLVTFEFLSFSESPTLLSNSIKKKVNPCNRQNCHCMVFNQFLASENSYINLRASSEEKQYLLAQLPNLGCTSIEVVKSSPESLEIVKTPLYQYKIWLKTIEVLCSQYNTIASISPLFKSQCKVSYDLLVTKKGQPAPKLPPPQPPQYQQASPPPFYSAVHPIPHLIYNQYPHGQNHLPYPPSVQPPPTPPQRVSKLSPPINNHETNNNSHHPAPPTPPLLNISHNIPQPPASMGPFGQQPPPPPTIPSKRISKSSPPTTPSKHASKLPLIINYGSLNNHSQPPPPPETRPISPPIPQSNILFGKQPPPPPPPPPSSEPTPTLLYSPPKPSNPNLKVITVPPKFKEYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZM0</accession>
    <name>A0A8J4PZM0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Right handed beta helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006520</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000305">
        <property type="protein sequence ID" value="KAF2072161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZM0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31318">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31318:SF2">
        <property type="entry name" value="PECTIN LYASE-LIKE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035147761" description="Right handed beta helix domain-containing protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="458"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="508" mass="54301" checksum="810C4085E5496F71" modified="2022-05-25" version="1">MKSLISFTCLIFCLFIVFSQSVAADGIKGSISLIVGNQGQSVNPCGQGGQVPVCASMADAYQTYLTMTQGLNYTSSLQFELMDGTYGSNFSPSWMKSLSIIPFSRTKGSVVFTGAYINNFVANNYTLSFTNITFSNAYGIIGATGAIDVEFDSCTFGNVNIDLTNPLLSLNGTGCSTPPQLVLNNCLFNNLTMGADLIDVTSYTVTITNTVVSNVCGGFYFTNSSTTISGSQFTQANSYSFATLLFEGGSATVKDSVFSENIVSSIGIFEAINSPLPSGPFLLDHISVNENTIQTIIPPVYIFNVQSSVVILGSVFTNNSFENHNSAGGAIQITLAPNIAIQYCTFTNNSALLNGGSLSISKSTVSVSFSLIDQSSSSVGGAIYVDDNSVVNIKNTSIIDNYSKNSGQNVYCKNSNISVYNSSINSYNHYGFSCQSGCSVDSDMGNICEHNSGLSKKVIIGLVVGLGGSIAVLFIVLLILRRQRNKRHYQRINDGDHHHHHHSNTSTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZT3</accession>
    <name>A0A8J4PZT3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AF4/FMR2 C-terminal homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002653</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000075">
        <property type="protein sequence ID" value="KAF2076039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZT3"/>
    <dbReference type="OrthoDB" id="24241at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043640">
        <property type="entry name" value="AF4/FMR2_CHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18876">
        <property type="entry name" value="AFF4_CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AF4/FMR2 C-terminal homology" evidence="2">
        <location>
            <begin position="708"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="845"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="84"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="208"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="258"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="416"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="469"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="482"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="505"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="532"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="555"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="663"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="677"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="960" mass="108186" checksum="D9FB04AF5E42556C" modified="2022-05-25" version="1">MNQDIGIHHQYNSSSSNNSFHSDDSLEEGEEIEERTSSAELDKKIFLLSNQNPHLKLKRTGDSAPDPQYGSFFNTLNSINQYHQQQQQQQQQQYQQQQQQQLQSSPTSTSSTSSISSSTSTPSSSSSSSKNRSNPSSTSKPQQAKIVNYTQPIPIKIREETSQQSKQEKPSSDKKSLTVSNIVKYMVEAFPDQLPMLSPPPNRAQENSSSSGSSTNSSSSWNLDDKKEHFIDHNQQEKMNESSKKESKSTTATNGHESSSNSSTNSANNGKEEKTKMKLILKLPKKQDSSNDLPTTTATTTKDKMDTTPSNGSTSKDKMDTIPKSTTTTTTNKEKDSGNSKDKDKDIQSKDPVKRKLDDKLSSVSIPKKGSATTTTTTNSKSSFVKKEDSGSDSDVNSNHTSDDEDGRKSKKLKSDVNTSSSSKSSNNNNNNMKSNGPNTTPSSKSNNNSSSSSSSKTNTPIKKEESKSSNNTSSNNNNTPVKKEEDNYKTKRKEDIPSNPFSVTEHDKKIIENEKKRLSPKSNEKDSLNTSSNSVRNDRSSSSNSSSNNSNSNNDRDRNDRDRDRSDRDRNDRDRDRSDRDRDRDRSDRDRDRDRSDRDRDRDRNNDRDRDRDRNNDRYDRDRNNDRDRRDSRDSRDNRDNNSRDSRRDSSRSINSDRKDNNSNNNNNSNNNNNNNHKDHSHESDAESVYSSTRPKKTKSEEKAIRSQIDFIKDEARDVKKIAKDLHSKSQEDCLEMFLQVGLKYIHVAQLMIEIDPITNITTYLSDTASFFSNTGKIAITTFKQESIASLCFKCESFCYAKNFYLKKEHLKHIKRDVQNFIVPNLSNSHSLSSSKLLAPAYPSTAPLTPTTTTPGSPMTNTNSGGSGNSNSTTTLSSSSSSNSNNLNLPSNILHKLTQYLRDTDDIFKSLDSWDRSNRSHLNDHQQPPNNNNDVQINFFYISYTDFIDHIKKKMDKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZZ6</accession>
    <name>A0A8J4PZZ6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_001551</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000037">
        <property type="protein sequence ID" value="KAF2077166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZZ6"/>
    <dbReference type="OrthoDB" id="264603at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033149">
        <property type="term" value="F:FFAT motif binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090158">
        <property type="term" value="P:endoplasmic reticulum membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000535">
        <property type="entry name" value="MSP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016763">
        <property type="entry name" value="VAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809:SF6">
        <property type="entry name" value="AT11025P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00635">
        <property type="entry name" value="Motile_Sperm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019693">
        <property type="entry name" value="VAMP-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="PapD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50202">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="MSP" evidence="8">
        <location>
            <begin position="19"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="143"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="188"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="235"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2077166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="312" mass="34514" checksum="D8157870F5ADCA10" modified="2022-05-25" version="1">MSSHSPNTSYSAETPSNSFLKIRPKELVFHPPFSGVIGNELKLINTSDHILAYKVKTTAPARYCVRPNTGIIPPGKTIEVRIFLNCTKEKDLPPLSMKTKDKFQILYIVVEDPSIDPKSIWQNVAPESIMKHKLKTVFTLPPTNVEQTNHSSPSPSSSKPYLQQQQQQQDSSSAYKSNNTLFAEVQNMESESDKSNFSSTFSTKSGQTTSTISTTDSDKTNTLAPNPTIKKRIINTTSSSSSSNNTETDDPIKLRNIIKDLNDKIEKLESLSKAESSSTSTATQRKPSIDSIQTLIVILIIAVISFIFGRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q013</accession>
    <name>A0A8J4Q013_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fatty acid hydroxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_003243</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000099">
        <property type="protein sequence ID" value="KAF2075462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q013"/>
    <dbReference type="OrthoDB" id="1658724at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="6">
        <location>
            <begin position="116"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="267" mass="31332" checksum="5C59B6C0853A9478" modified="2022-05-25" version="1">MSLAFLEPYWYSFVDKFGEDFLISYGTFFAHQAFYFGAFIPFFICDFLPFLQKYKIQQNKTNDWKSQLNCTFKVLLTQTLVQLPMMIIFHPAIHATGLRAKAPLPSLPYLLLTLTCSFIIEDFYFYWVHRALHHGIWYKYIHKMHHDHSAPFGITAEYAHPIETLILGVGTVIGPFLFSRDLFTLWVWLGVRLYQTVECHCGYDFPFNPTRLIPFWGGAKFHDFHHETFVGNYASTFTYLDAMFGTSDKYLKRLDERAAKKSQTKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q042</accession>
    <name>A0A8J4Q042_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Amine oxidase</fullName>
            <ecNumber evidence="6">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_001445</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a secondary aliphatic amine + O2 + H2O = a primary amine + an aldehyde + H2O2</text>
            <dbReference type="Rhea" id="RHEA:26414"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:58855"/>
            <dbReference type="ChEBI" id="CHEBI:65296"/>
            <dbReference type="EC" id="1.4.3.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the flavin monoamine oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="6"/>
    <dbReference type="EMBL" id="AJWJ01000034">
        <property type="protein sequence ID" value="KAF2077256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q042"/>
    <dbReference type="OrthoDB" id="5046242at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097621">
        <property type="term" value="F:monoamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001613">
        <property type="entry name" value="Flavin_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050703">
        <property type="entry name" value="Flavin_MAO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43563">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43563:SF12">
        <property type="entry name" value="FLAVIN-CONTAINING MONOAMINE OXIDASE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00757">
        <property type="entry name" value="AMINEOXDASEF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="7">
        <location>
            <begin position="13"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="33"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="422"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601613-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2077256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="454" mass="50609" checksum="5A22B7403BBB5062" modified="2022-05-25" version="1">MSQYDCIIIGGGLAGLKAAYELKKSGSSVLVLEARNRFGGRTESTKVDDYWFDLGGQWMGGTHKLLQETCKELGIQSFPQYDEGKHVLEINGKKVYYSGNISTLNKDYNLEGLSESIAKIDELASELDPTKPYAHPKVKEWDHMTVTEWTSINVPGKDARTIINWFIRVCLAAEPSEISFLWFLHFIRTAGSYSLLADIHGGAQQDRLIGGSQQISEGIAKKIGDTNFCLNSPVRSIYQDANGCTVKTDLTTFRSKFVIVAIPPTLAGRIEYKPTLPHARDELTQRMPMGHVIKTIVIYDEPFWRKDGYSAEALSDVGPIYICYDDSSHDDKKTAIVGFIAGQPAREWSKKTPEQRKQAVLDCYARWWGPKALKPTHYLEKDWKNEEFSRGCYLAYAGPGVLDSCGAALRTPVGRIHWAGTETASAWIGYMEGALESGIRAAKEVKDKLLSSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0Z8</accession>
    <name>A0A8J4Q0Z8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cell differentiation family</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002606</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CNOT9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000073">
        <property type="protein sequence ID" value="KAF2076092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0Z8"/>
    <dbReference type="OrthoDB" id="1183224at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007216">
        <property type="entry name" value="CNOT9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12262">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04078">
        <property type="entry name" value="Rcd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="122"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="321"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="727"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="325"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="361"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="732"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="765"/>
            <end position="774"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="801" mass="90880" checksum="2761337EE26A7D0C" modified="2022-05-25" version="1">MLNSHPPQSSSSSFLPLNKSESPLSSLSTPFFPNIGSSLINSNNSQHNLNQGGTKDFIYSTLENNFYEKLGINGNSSIFKNDTLINNNNNPNNCNFQYSTQQLNSEFPLTQISTENPPFLKNNNNNNFNNNNSNNNSNSSFYQNGNFLNSNQESKDYFKQSINSNSCNTPISSLSKLDSFNVKFNNNNINQKANNSNYFQPQQQNSLYSLNNNNYKNYPSHSTTSNINSIQFENVNNENYIDTIKISNEAIHQSLQTMIVSSEASTPIISSPLGSPSNNSLNFGFSKYSPSKEQQQQLESINTQSLFSTYDLDSAYSRSPSPIFNINNNNNNNNNNNNFYQPPKQHQLIKSQPPPLSPLHNNNNNNNNSTINTTTTTTTSIKSNTTTNNNNNNNNNNNNNNNNNNNNNNNKSLNNNNSFIHSNNNNNFKENNTKHNNYRNKSGGGNNLRIHKIEDKSTNQLIEDLIEFDKREDCLNELVKRKDSIPNLANLIWFSPCVVSVFLQEIMNIYKYITPPNPKLKARASNRVCNILTLFQIVASENDTKDLFVKSQVISFIYPFLQTISKSKPFEYLRLTTLGVISSAIKQENNGIISFLLEIDISTPCMRIMENGNDLSKTASVFILQKLLNEDEGLSYFVQNSKRLQDLLLCLQNMIKSMIPDHMSSRLLKHIVRCYLRLSDSLKVREFLPRSMPECFSNGQLNDFIQKEGDVIVKRWYTQLMSNLYPQNSKRTGASNVGGSVVSTNNNSNNNSNNNNNNNSNTIPHHPHPHHPHPHNQQQLYHHHHHNSAASSNSPRKRFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q166</accession>
    <name>A0A8J4Q166_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002757</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000079">
        <property type="protein sequence ID" value="KAF2075954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q166"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35885:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35885">
        <property type="entry name" value="CARBOHYDRATE BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035206148" evidence="1">
        <location>
            <begin position="21"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="308" mass="35000" checksum="7E16D1E81FB607F1" modified="2022-05-25" version="1">MKLILLSLLVFSVFLSFSNATDSFSVGIWNDDTSINIGFVDFNGNETSKQLVIPGFYYPSNTNQTSTYNFNTNILTFIAETVKYKKPFLIIVDCTSWQVVTNVSLVFGYGYVGLASDQSNRNIVYGFYYKSFYENILYGIIIQENGKISYAYQKVFGSYKSSVFNTKTENYLIAFQNSTGSFMRSYSFNFNFINEAPLTFSNNKFEILNEPFPMASVNGTTLGTIPMKWIGDKPVFGLAYLDSTQCHFNFTKMIAYGTDTITTSTQRDSFIFIFAKSVGGQFYLYTFSTLSSSFLSYKLYYTPILSVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q185</accession>
    <name>A0A8J4Q185_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_000569</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000011">
        <property type="protein sequence ID" value="KAF2078098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q185"/>
    <dbReference type="OrthoDB" id="15554at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PRAJA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710:SF234">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="73"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="135"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="155"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2078098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="165" mass="19986" checksum="10FDA664E014CCA2" modified="2022-05-25" version="1">MTSTRESQQQRNIREFMENYTINDPSANQQEDLERIRFILKHRPAPPVSEYQFNEMTEEVIITNRNKERIGDCTVCCFEFPIDTEAIKLPCKHYFHYDCITQWLNLHSVCPNCRFQLPSQDFEYDQMKRIVDQHDKQSKDDYYDDDERGDKKSYYSRSKNSYMYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q190</accession>
    <name>A0A8J4Q190_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001303</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000030">
        <property type="protein sequence ID" value="KAF2077375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q190"/>
    <dbReference type="OrthoDB" id="13806at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="136" mass="16222" checksum="98A8A4D7EC0E009A" modified="2022-05-25" version="1">MKIARPLSGLFKKQPKRRMDHAPLNELSESRYKVEQFVSEDPFTKGKYTQHRILSVCVDGIKVITSCESDVIEEIFWKDILQFNLKESWTEWEIVLKDRRKLYFKCEKAFDIHCKTDNILDGIIRNNRSNDFSEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1J6</accession>
    <name>A0A8J4Q1J6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rab GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_000453</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000008">
        <property type="protein sequence ID" value="KAF2078263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1J6"/>
    <dbReference type="OrthoDB" id="1436450at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045335">
        <property type="term" value="C:phagocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090385">
        <property type="term" value="P:phagosome-lysosome fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01862">
        <property type="entry name" value="Rab7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000086">
        <property type="entry name" value="Ras-related small GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981">
        <property type="entry name" value="RAB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981:SF20">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB-7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0636">Prenylation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2078263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="22737" checksum="52BD68766F758DD5" modified="2022-05-25" version="1">MATKKKVLLKVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKELMVDDRVVTMQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNVAKTFENLDSWRDEFLIQAGPRDPDNFPFVVLGNKIDLENQRVVSQKRAASWCQSKGNIPYYETSAKEAINVEQAFQTIARNAIKLEDGLVFPIPSGIQIQPEPTPAKSGVCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1N6</accession>
    <name>A0A8J4Q1N6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acylamino-acid-releasing enzyme</fullName>
            <ecNumber evidence="5">3.4.19.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_002399</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of an N-acetyl or N-formyl amino acid from the N-terminus of a polypeptide.</text>
            <dbReference type="EC" id="3.4.19.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S9C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.1" evidence="5"/>
    <dbReference type="EMBL" id="AJWJ01000066">
        <property type="protein sequence ID" value="KAF2076284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1N6"/>
    <dbReference type="OrthoDB" id="416344at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008242">
        <property type="term" value="F:omega peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045550">
        <property type="entry name" value="AARE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776:SF4">
        <property type="entry name" value="ACYLAMINO-ACID-RELEASING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776">
        <property type="entry name" value="SERINE PEPTIDASE S9 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19283">
        <property type="entry name" value="APEH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acylamino-acid-releasing enzyme N-terminal" evidence="10">
        <location>
            <begin position="11"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="9">
        <location>
            <begin position="486"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF19283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2076284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="699" mass="79167" checksum="22D32BE78378E418" modified="2022-05-25" version="1">MDNNQYRDECVKKLVEIVSIPTIKKASFSSSNSISIILTNTDLYQKKTKNYLLHKHVINNKQVISTCPQELVSTDIIKSPTGLKQIVFKEQKNGDNTEYQFEIFENNQYLKTIVSKDLLKKICFDDWFGAPQFSPCENYIAFIGQLKKNTGSFFDKNPKDKIIGGEYIYSGNWGESYQELSDLSIFMCDLQNDKVYQLPFPTDQIAPGQIQFNPDGKSLVFVGWSVEKRKLGIKLCYHRENNIYQLNFEDNPKVTDPDSPPLKLINLTSQFPSGAFRTPRFSPNGKLLVFIGMDEKTLPHNTCAQMFCLNIETKLIETLIDYKNNHDEFTGIYSVGLPRQCFINENTVLFNSSNRSVTKVFSINIETKELVNLFPTSLKGSTIVETNLKTNEFILSESSLNSPTKLFLVNYKDLGSKLLIYEPILSNVCQEAFNSFDFRLEGLESKEGYKYNAIVIESKNIDKTGTILFLHGGPHLCSTTDYLYYLSFMASLGYNIIIPNYRGSTGFGKDFIDVLNGNVGIYDLSDCVDSLEKIKKQLQNSNEKNGILGGSHGGFLGAHLSRFKFNAIALRNPVIDIVSLSVTSDIPDWSFYEAGLKPHGTNKNYNAIPTLEELNKMRICSPSNFIDEIKESKTPSLIMLGENDIRCPSAQGLMLYNSLMESGTDTQCLMYPKGGHMLDTIECKDLWIHTALWFKKYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2Z9</accession>
    <name>A0A8J4Q2Z9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001508</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000036">
        <property type="protein sequence ID" value="KAF2077187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2Z9"/>
    <dbReference type="OrthoDB" id="17128at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="450"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="450"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="469"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="483"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="544" mass="61526" checksum="6651EC6D16D55D33" modified="2022-05-25" version="1">MAMSWISNKFKSVVDAIEKIDNKIPRNDSDNEGDNDNDNSSGNGNNNESKYKKSNIAENQLSLEAKKMIYDAVSPYTQTPHGCISDFMVNLYGTGSHSQCFVNKEAVDIFHNPINTLKFKSMASGCGVLSVNEFDTQYMYRLVSLVRNLRRFSYPFGKHMLCSIDQRYDYSGEALPNLTGYYVPNDYLFDIVNDYKDLFTPCISIHPYRKDCVDELIKYAVLGVRYVVWSPISMGINPSSELCDDFYETLIQNDMILFIHTGDEHVLVDAPESDNKWGNPLLLRRPLNLGVKIVAVHSGGEGKSQDIDEKYPHPLVPNINLFLRIMHEPRYTNNLVGDISGIVAHGRVDSIIPLLDHQNIHNRLINGSDYPLPAVNTSVLTILLAKNGLITSEERDIVNEIYNYNPILFDFVLKRILRSPSGKQFCLSIFRTNSFFDLKDKSNILFDSGKSSPLKSMPSSSTGSTSSSNWNELSPQPTSPDFNSTLSNNNNNNSNNFNIDDVHNDDTFFDEELNNSMNNDGEFKDVIENGYNHEHPSLEEYSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3B2</accession>
    <name>A0A8J4Q3B2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000012">
        <property type="protein sequence ID" value="KAF2078079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3B2"/>
    <dbReference type="OrthoDB" id="78088at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039874">
        <property type="entry name" value="WAPL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22100">
        <property type="entry name" value="WINGS APART-LIKE PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22100:SF13">
        <property type="entry name" value="WINGS APART-LIKE PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="466"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="75"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="301"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="466"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="478"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="502"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="535"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="561"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1114" mass="123720" checksum="FAFC990519B4026A" modified="2022-05-25" version="1">MSFFKRNKEEEEKESDFWNKKASSTKSLFNLSSSSSSTSTPTSPKKVVSSSSSSSSSSPKKAFQPWVLPAKNGPSSTIVKNNSSNSNSKSITKEKETIKGSPWINSLKSGTSKEKEETTSKQKESVVVATTTSINKDHSIPKQVKSPTTTSIVEITPESTPKPKDSVAPHIIVDEKDGEEEKDTMVEKVVNSSLSKLDQKDTAIKIDENLDDKTKSDKEKLALINQNTISIKEEKKKVGSIVKSSSTVPLVIELQSNAIPGGKPKPPPKRSATEIARISKEQQEINQKLLVIKKKKERKQTTTTTTTSTSLVPPSSASVGGLSSPKPSLSTNSTNTPKVKLDKKKSTAKLNNEKDTDDEDQSMMTGVLSQTIKENQNYYQEAKKIVSSLKSLDSKVILDSLNKLVEIVTKDTTEFTLILRSHNLFSNLTDTLIYLSNLSIDNILLSSPSTTTTTTTTTTTTVLNRSASLVTSSPTKLSVNEPSSSNNNTPKKSPVKRSNTFDSIDSIKSKSSPTPRKKNRAPITKSEDLLSMFLSTTTPPSSQSSSQTAGTPPSSQSLFDQDSDSDSTDDEQNDWKPLFLQKRLKSDPSSLMNQKENNAEKTAINIATCFIFYAFSTNQVNIEHFNRDTIEFLYKQSLTIPENYLFEFFTTPSKLPKSPMSPISPSKKQQKKSELLINVYAQFPNLVHDTVRFSVTCPSLSLATLSNLASNHINHSMKLKLKEYGVMNGMVQLLYKYIHYLNPILDIGSISPKLIGNIENSFKILNQCLVGDIKAHREQLLSMETLQLFVTYLRYLDIIITSNTDRLVGIDIKSIGSDTIISILEILLNITHENNPASHYIATLNGPYTNLGLPSNVSPSLKKSSKPEAFVPTAVDHNNKEFTQYLDSIIQNTPTKLSPPLNGISPSTTISYKLTPKKTRFLESIELKEHFGFKTLLSLLKLEVLDKYDVGQNCVSLLVNLVECNPENRQIFGSIDGAIEVILNIYLWRTSGRILTRFTSKVQEMIASSYIVILIGFLIKDNEKNRRVVLDSLPKHSFDDMVILLQDFIEFQINTGILSNQIYNMVIHLAGEVFIQPNNNNNTEFIEVSQETIDNFVKFKKQSDVKINNSLDPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3Z0</accession>
    <name>A0A8J4Q3Z0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_000291</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000005">
        <property type="protein sequence ID" value="KAF2078424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3Z0"/>
    <dbReference type="OrthoDB" id="5586at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21504">
        <property type="entry name" value="PPP2R3A_B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.220:FF:000003">
        <property type="entry name" value="Phosphoprotein phosphatase 2A regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000025">
        <property type="entry name" value="serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041534">
        <property type="entry name" value="EF-hand_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14095:SF0">
        <property type="entry name" value="MIP22305P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14095">
        <property type="entry name" value="PHOSPHATASE 2A REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17958">
        <property type="entry name" value="EF-hand_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="571"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="63"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="192"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="697"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="76"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="229"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="701"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="751"/>
            <end position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2078424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="761" mass="88751" checksum="686E1D3A46202AD4" modified="2022-05-25" version="1">MHDLNSFDEKQTNTDCIINAKLKITELFLTWLSNPDTGHYINDLLDGINNDFNILDTNVKVPTDGSNPNGISSMESSPTSTTPIQDTASTTTDTDDNQHIDDIPTTPIITVSHHNTPDDKMDISNDTTTTTTISSPVQEDTLVPAIINQQQQSQPQKDTQDVKMEDINQPQQPPPPQDVKVNVLEEKQLEEQIVPEKESVQQPDVKLQQPQESVQIIKEKQPQQLDFDQETKSDYHESTFDSTTPTNESASPTTNASEFNSDHEKDEEEEQKIVVPQFYFPRKTNLKPVSDRLRAYIKKRFSSFGRKLTNEQIQQQQSPSSPQLTSLFFIDTFVEFELLIKGFWSYPRILNRLLYIHIISNISEPYISSESFIEYWRNNINGKEPEEILFNILKKSPDSQYLTFEDFFLFSKTLTETHPGLEFLKNTPEFQDRYQETIVVRIFYSTCKNRGRITVKDLKSSNFIKCLHLLDAEPDINKHLDYFSYEHFYVIYCRFWELDTDHDLYIDANDLLKYGKCCLTCKIVERIIENRIFTKDVSHNHNNNDITNCPCFKKMSYQDFVWFILSEEDKTSDTSIEYWYKCLDTDGDGFLSLYEMEYFYQEQKQRLDYLNLDPPTFSDLLCQILDMIKPKSIEKISISDLKKSKMAGYLYNVLFNITKFLQQESRESNSCGRTMSDWNRFALQEYEKALFEEVIHQSGDIVDEYGEDEDDDEDEEEEDEEDEDDDDEDEDGTSDYDPTLANGTLSNYNDRDDDDDFDYED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q802</accession>
    <name>A0A8J4Q802_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ras guanine nucleotide exchange factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_002352</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000064">
        <property type="protein sequence ID" value="KAF2076346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01765">
        <property type="entry name" value="FERM_F0_F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014768">
        <property type="entry name" value="GBD/FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010473">
        <property type="entry name" value="GTPase-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06371">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01140">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51232">
        <property type="entry name" value="GBD_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="GBD/FH3" evidence="8">
        <location>
            <begin position="673"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="7">
        <location>
            <begin position="1425"/>
            <end position="1546"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="6">
        <location>
            <begin position="1580"/>
            <end position="1808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="412"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="534"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="623"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="726"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="418"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="534"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="733"/>
            <end position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2076346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1816" mass="204370" checksum="A976A922DDF71A7F" modified="2022-05-25" version="1">MGNINTIVNSGGGKYRIDHLSFLLEECKIKKKQVVEHIENLNNAYPMIQRLEITSCKLKELPANIRDLVHLSHLILVDNKLSSLPWSLPFPVSPQASVTISSVVSEGLTKLDLSHNHFSSFPSCVFVLPNLVVLNLDHNQLSDINVTTVNTSWVTPTTTPKPYIVCKLRELRLSNNNFSAFPDIITELTSLQLLDLSGNSLSTLPASFTNLANIQTLNLKTNKFTSFPTSICTLGHLSHLNISHNQITIGKDHHHLGISLLPSLTSLELQHNKFSEFPMDVCDITSLKVLKMQDNDIDSIPEKIGSLVHLQDLFLQENKITTLPASFGNLVDLKKVFLEFNKITKLPAEFSKLTKLNVLIMHNNELKVVPPEIFALKQLVRLSLDDNPLSPQELKQLKTIDGALNLVNQQQKVTNNDSISGSSGRNRGNSVSGGSISSSTGSFSPLSGKPKKKPGHLTISRSLFRGHSSNLEGEKDKFKKQQQLKEQQHQHLQMISEDNDTSPVQQSPITSQITINSAFMEFEDEIQKMINEQNSNSPVTNSEGRSRRSSVFLPPTPSIHKNSQFSSLPSSISQFLNQNQSLIQQAAASGGFLDSPSLTPSYSSPLLSSLSASSVGSTSSSASSGAILAPSSSSSTPTLPSGISQLSISTGYTPPTSSASSSSSSSSLSTSDGSIPSYSKFKGTFDQLLEEQDFSKKKRETLNKLSKEQKWNLLLQYKNSTLKMLSLKNPQPTSGTKSSSSSSSSTNSTPTLSSNNTTTTTTSSTTSVSRNTTFQSAKYYFDNLDTTDISLVKELLEFLKDSGPEVSLFIGYGGIEAITKILKYNLDHHSDAFEEEKVGFCLLCIKYLVEFAPKSVITTNNSINIITMFLNSDSVHIVTIILEIFDELLKLQNIGSSGGINNINNNFSVVIEALNNFKKAKGLRNPYVPLIALLKNQSYDLSTKALLFKIINNLIYSFEVLEERFSVICTMLKLGVLEVVQQLRNFKNNEFLKFELDMFEEVLDSDENELINKIGRREVLKRMSNEYDISKQSEETCDFVRVVMVSAGLGDLKINLNEKTSYSEVIDFIQSHYQIKYDLSRYGLFIPGIGNETNQSGVNQSRWVDPSKSLIENGLSGEIVEFRMIPNPLNVYQSIQDDKKFKTIVIDPQLKCFEIVNYLRSRLDPVAPAFSRKVSTLEEIEEEEDTESDIYGIYCFKSLQEYDQGTWYPQNSRFLDFDFNNIVEVKLKERCLRITFSGTEKLMKFDPNQLVSEITVEIRKEIGLKINVEDYGLSYTPTVGSLASSTHDGFWLESDKRLIDYSIMKNSPVKFKFRPRFLFVRVTAESGISVPEEQNRGLNNTLPISEIIEELVEPHGLNPNEYSLYLSNESNQKIKLLSRHNCLRDQQVVKDGSHILITSKQKDLPSDFNVWDEPEDSPETITYTNSNKVASITLNKLIEKISIPESQDAYLRNTLYLSYRSFITPLILLNKLVERHSIPSSVDVEKGKQIQRKVRSLFKNWLDNETPLNRLEVIELMNLNLPSQTAEDDGSKELYDLFIELTNNSNYTKHMNTDNNHLEKPKSKIPKHPHDSFISLIEIDENELARQLSIMIFPLFSKIETSELINQKWCKAPGQAKNVMEFVNFFNRLSNWISFNVVNTSKLRDRAVVFSKFVKLAQAFCDLKNFHLLMAVLSGLNSSPILRLKFTKSKLSKHSRQNLEDLEALMSTSSSLKNYRQILAETQPPCIPFMGCHLSDLVFIDEGNQQMYGNQINFKKLDLYAKTLTTLQKYTIVPYQFHPVPIIQNLFNDHRIVEEKALYEMSLKCEPRNADRSDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q9R8</accession>
    <name>A0A8J4Q9R8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Leucine-rich repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001117</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000025">
        <property type="protein sequence ID" value="KAF2077576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q9R8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="203"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="37318" checksum="DC05CE086D6F22AB" modified="2022-05-25" version="1">MVTTTTTTDTNSSIVESLLELADAECLEDIVNLDLNNQPNCSQYFYLFSQLVNLKSLLLSDNFLQEMPPLSLLTQLEELSWRNVQVMNRDKSNLLNQLQKLKIKHLILLDSKENDFKDAFIFSIKTLETLNFKRVTDQNRKDIKDRLLQQKGISPPTTPIKSNNISPTATPINTPSPTIKNRGLALKPSTPTTPSSIPRNQATLPTSTTKKTPISPTLVKYTTPIKSTIATPSRPTTMKSPPLRSTTTSPTTTPRPTTTAIITKPLASPTPLSSKAPIKKQVNFDMNKNEQQSPTIQPQEQQQQDKIIGAIKLLIDTLDVNQLKEINGFINKKMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4QAR6</accession>
    <name>A0A8J4QAR6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Asl1-like glycosyl hydrolase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_000602</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000011">
        <property type="protein sequence ID" value="KAF2078131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4QAR6"/>
    <dbReference type="OrthoDB" id="15846at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053183">
        <property type="entry name" value="ASL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024655">
        <property type="entry name" value="Asl1_glyco_hydro_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34154">
        <property type="entry name" value="ALKALI-SENSITIVE LINKAGE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34154:SF3">
        <property type="entry name" value="ALKALI-SENSITIVE LINKAGE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11790">
        <property type="entry name" value="Glyco_hydro_cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035226325" description="Asl1-like glycosyl hydrolase catalytic domain-containing protein" evidence="2">
        <location>
            <begin position="26"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Asl1-like glycosyl hydrolase catalytic" evidence="3">
        <location>
            <begin position="48"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="280"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2078131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="345" mass="37184" checksum="B504057299BB9977" modified="2022-05-25" version="1">MFIATKNKLIYLTLVLVVCGAVVEAVSKKGTTFGSDDKGDLAICSDIRDLKVTWYYNWDLKPNCNANANIIPGVQFYPTIWGAANLNSNTAKNIAKADVLFTFNEVNFGTQANLKPTDVASKWPDIEKLGFKYIVGPSVADCPDNSDPNGQYCNYGGVKWYKDFFAACPNCKVDYVNIHIYYCDADDVMNKITNLNKATGKKVWLTEFNCDGAKDDTAVLNYAKKLLPLLEASDIVAGYAWFLPYYTEGNINSLYLNQNGKGDLSPIGKYYASVVSNPTTSTSSTTSTGGSTSAGSSTSSSSSTSTPSPSSTGGSTTSDQNTNGATKSTISIILLSFILIISLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4QBT1</accession>
    <name>A0A8J4QBT1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Inner centromere protein ARK-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_000027</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the INCENP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000001">
        <property type="protein sequence ID" value="KAF2078656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4QBT1"/>
    <dbReference type="OrthoDB" id="6123at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005635">
        <property type="entry name" value="Inner_centromere_prot_ARK-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03941">
        <property type="entry name" value="INCENP_ARK-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inner centromere protein ARK-binding" evidence="9">
        <location>
            <begin position="958"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="390"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="491"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="579"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="720"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="816"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="239"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="400"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="494"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="588"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="659"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="733"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="769"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="816"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="846"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="884"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="908"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="936"/>
            <end position="950"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2078656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1059" mass="119324" checksum="BDBE84716CF64718" modified="2022-05-25" version="1">MSTFRNRNQSIMTPHKSSISTFKTPSKSTTLNSSTLVNNIKKNFELENNNTSNSNNNNNNNSSLIQTKNITTAAAQAAASTITSITKSQTMDQHLKSIDNHFIEKGKLARDHLDQFSNNILDSMESLKDTLIQSIKEPESLFTLPIYPSTLKSNNKSLYKKLNSVPEPLYSGLILPVSTHNIKIKQRQAIIEQQESEDKHNQQKHLYELSKAQEQQKYMDLLKQQEEFKQMESLKQQEFVKIQQELEIKSNNVNKLNETLMAKELKMQDIDKQQIQQLEKERKHMQTEFEKMKQQQQKIIAEQQEQLKIQKQKLDSQQSKINQDQEKVSLLSNQVSKQQQEISLQKDEITKQKQDLSKKQIEFDKKEKELKEKELAAAAVVIAASKHSLKPAPTTNHSGTTAATKNISTTKTTTTSSTLSKPTIGQAKQPITSKLAAKSTATTSATTTLSKSKIDQEYQNILSDLDNNTKTIKELNTKKLENLKQQLCTSDEANLSSNSSNSSNNENGDVDLSSTLQNMDVISNTLKRKDLNENSTTEVFKKRLSEDNKTILSTKSGLAKKDTNVPLVAPKKTLNSTITATTSLAAKRRAEEEEKKKKEKEAEEKKNALKKQKEEEALRLKKEQEEAKRLVQEKAREMQEMERKREELRKKEEEARQKILQTSLQSLLPNTTPTRPANSSQSAQTPPDASPIALSESAKKPNTYLNGVLSYVFGIAKSPYFKPSTATGVDTSSEAEDDSDENSDEVEMDSEEYSEEEESIEYETDKTPNKNTSNNNNNKSLDSPILSPLSITPPPTKISNQNKSISFANNISESYCSPASSISNNSNSYSGLSPSEITATPPPCKNYSQPSTISPTLTLRAGSSKNSPIKFNPLGSPHNNSKRSSQSSPLSNTSSDNSFKKPSHSSRFENISFSSASSLSDFDSSEDEQEEIKPYQQKQVAQTKTQTGVQYPIETSPEKEDDDNYLYEEEDIDYDYDRNVPDWAKNPFLSTTLKQQKFCDPDKIFPDVGPVYLFEIFPVNQVHQTDFKNIKRNLSSDWSTDEITPFENSNYKSVMGFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPU2</accession>
    <name>A0A8J4UPU2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008962</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000607">
        <property type="protein sequence ID" value="KAF2069721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UPU2"/>
    <dbReference type="OrthoDB" id="10489161at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="202" mass="23232" checksum="4E119BB7CA4A6B5D" modified="2022-05-25" version="1">MVLLKISKVIQKCSTPLILFPSLVMSYYIFFAISEITSQLNAPVLDSFLRGYTREQAISTLTQLGTQGRLQYSTIYTKGYDILFPITSFFMFASLLSKTYPVLGIKYTQMFNIVPLLYLICDLSENYCHYNILVHFPNAAMEPFIKYGSIFCEFKYIFFGLSFLLLLIGFLINLTHSIWYFCCASKQTPRATPKSTNSKKHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4URV4</accession>
    <name>A0A8J4URV4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005904</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000248">
        <property type="protein sequence ID" value="KAF2072781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4URV4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035216241" evidence="2">
        <location>
            <begin position="18"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="159"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="374" mass="37531" checksum="8E0D3545E1C88572" modified="2022-05-25" version="1">MKSILLVLAILVALSVAQNYDCPYSNIPPNAANPTHSSVTLTVQLCWDDINTCSLVNLADGNGLNMVPGQAPVKVFITPQATRYAYFIFTGSEICVSHSLYKNTVNCGVSNTLECKLECISASLLYKSVLYTTAYGDKCSVNWNDHMILSWKAVPLTNGTTSSTETTKGSSTGGNSTSTTGSVTTGGNSTTSETTRGGTGNSTSTTGSFTTGGNSTTSETTKGSTGNSTSSTGSVTTGGNSTTSETTRGGTGNSTSTTGSFTTGGNSTTSETTKGSTGNSTSSTGSVTTGGESTGNSTSSITSGSEGSGNSTSSTGTGSGSGNSTSSTGTGSGSGSSTREYVPPVVTIDPSSSSILSISASLFIFNLFALYLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UT67</accession>
    <name>A0A8J4UT67_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004196</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000144">
        <property type="protein sequence ID" value="KAF2074491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UT67"/>
    <dbReference type="OrthoDB" id="2556847at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053287">
        <property type="entry name" value="PP2C-like_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21586:SF2">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21586">
        <property type="entry name" value="TIPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="292"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="329"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="696" mass="77092" checksum="3F4859A63B80888B" modified="2022-05-25" version="1">MKVKEKRKSKNKTQDNIKKQSSLSVLESSSSSKRNSNSDINNPGGDSFSPIILASKSLSTGSIESSTTSGDITPPFNNNIDHSDLQATLTSPQTSLNISNSSISIIAMSATTTATNYPNGNSSMNNSNNTTTNNTSAAQQQQLIKKQQEQQEMEITKTFLSEIQKLERLLQDLIIQRNTNTRKPIISTDEREFVREIENDSNDAELKREEDLLKTIIESKKADFRLFVAASDKNNKNGTIIKKSAFLKSGAALVIFPTVVEEKFCSSLPLIDIETCSNSNSNNCSNRLSVNSNGSNSSINSSNSNKPVNSNNNSSNLDESSDTNHDTTDNNNNNNNDNDNNDKDDIISTICGPLPDLPEDTHVYKDLICAKSGSSYPKHFTGRRIGDPICDRFKVLIFKDKLIVSLADGCNWGRLPYEAATKATDSFLLFLEENVHEINTIRKAGSFLLAAMTAAHKGIIAGKAEVYESGTTTLIGGLLTRIKSESNIKRMSSLTYMGKDHVDATWVFTGVTLGDCKAFHYSKKHRTFSDITKGNRQNLTDARDPGGRLGPYIRLGEPDLRNLEVFYKFCDQGDLILLLSDGVHDNLDPQQLGVNPTELGIEADTWEKAGQLFPSETEDAKSDYRKRWLSDHFQTEEQLEPTFISNTLLKHCTVTTQSSRDFMEQNTSKKLPSDYTLYPGKMDHNTCVCFKVGSFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UV46</accession>
    <name>A0A8J4UV46_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Deoxyhypusine hydroxylase</fullName>
            <shortName evidence="10">DOHH</shortName>
            <ecNumber evidence="10">1.14.99.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Deoxyhypusine dioxygenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Deoxyhypusine monooxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_010395</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate produced by deoxyhypusine synthase/DHPS on a critical lysine of the eukaryotic translation initiation factor 5A/eIF-5A. This is the second step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine. Hypusination is unique to mature eIF-5A factor and is essential for its function.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>[eIF5A protein]-deoxyhypusine + AH2 + O2 = [eIF5A protein]-hypusine + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:14101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10144"/>
            <dbReference type="Rhea" id="RHEA-COMP:12592"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:82657"/>
            <dbReference type="ChEBI" id="CHEBI:91175"/>
            <dbReference type="EC" id="1.14.99.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="10">Binds 2 Fe(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; eIF5A hypusination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the deoxyhypusine hydroxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.29" evidence="10"/>
    <dbReference type="EMBL" id="AJWJ01001082">
        <property type="protein sequence ID" value="KAF2068280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UV46"/>
    <dbReference type="OrthoDB" id="421002at2759"/>
    <dbReference type="UniPathway" id="UPA00354"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019135">
        <property type="term" value="F:deoxyhypusine monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000099">
        <property type="entry name" value="Deoxyhypusine hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03101">
        <property type="entry name" value="Deoxyhypusine_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027517">
        <property type="entry name" value="Deoxyhypusine_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004155">
        <property type="entry name" value="PBS_lyase_HEAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697:SF5">
        <property type="entry name" value="DEOXYHYPUSINE HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697">
        <property type="entry name" value="PBS LYASE HEAT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00567">
        <property type="entry name" value="EZ_HEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0386">Hypusine biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="HEAT" evidence="11">
        <location>
            <begin position="79"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="11">
        <location>
            <begin position="234"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2068280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="324" mass="36213" checksum="77DCE1EAE2C2CCBC" modified="2022-05-25" version="1">MVTITDNKVLVTQELVDKLKATLLDESQPIAKRFRSLFTLRNLNGRLCIDAMCCGLNDKSALLRHEIAYCLGQMEDDCALETLVKLVKDSQEHPMVRHEAAEALGAIGNPVALNTLKEYSTDPVKEVSETCQLALSRLEWYTQNEPQSVENKVYLSVDPAPPHPANTYTHQQLREQFLNQQLDIFTRYRALFSLRDKGDEASVLALCESLAHDADALIKHEVAFVLGQLQHRAAIPSLTNVLLDESESAMVRHEAAEALGAIASQETVPLLEKLCQDKEPIVSESCLIALDVTDYFNNTEEFQYADGIKILLEKQHSAITDSHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVG2</accession>
    <name>A0A8J4UVG2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009828</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000822">
        <property type="protein sequence ID" value="KAF2068848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVG2"/>
    <dbReference type="OrthoDB" id="24009at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="529" mass="59338" checksum="6B80DD170796C8F1" modified="2022-05-25" version="1" fragment="single">GCPDLPAVKVPPSVTRLTLSSTRWQSLCEIPATVKYLSLDRLDGPQFIPPSVTHLTLLDQAINSLEIEIPSTVQKIEFKKLSIKTYCSLDIPSSISNRLVNGVFYIYSESNNKTTIPSNATHLFWADDHLIDNDEVNIPPSVHTLVLGTNFEAPIISLPPTITQMVFSGELQQPLETIYFPPSLKYLYFNDSFLHDIHRNDLPNGLTHLVFGNFGGQIESLPQSCHHLEFDYQGSSDLDESNVIPPQIKHLKFTSDYPIKIPHTVKSLMTFSHDQITFIDTIPPSQSWNNVSIKQPSPFISTTRIHLRAPQYSNFNRFIMPNRLGNNIRSIAFGDMFNQGIVKGTFPNSIEHLDFGNKFNQKLKRSLLPTSLLTLVLGNAFNQDLNDVFPSTLTEISLSMLSKPQIKSSSQFPPNLKKLTIPCYDGVLDIVPTSTINYLQFNNNIVQNATDSLIFPVELTPPNITTLILNDKLYIESYSIIPPTIKNIKLCLSMVPDTKIPCTIESVVLPALFNQSLDTILQTVNEDDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVU0</accession>
    <name>A0A8J4UVU0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chaperonin 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_009575</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the GroES chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000747">
        <property type="protein sequence ID" value="KAF2069103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVU0"/>
    <dbReference type="OrthoDB" id="184876at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00320">
        <property type="entry name" value="cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.33.40">
        <property type="entry name" value="GroES chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020818">
        <property type="entry name" value="Chaperonin_GroES"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037124">
        <property type="entry name" value="Chaperonin_GroES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772">
        <property type="entry name" value="10 KDA HEAT SHOCK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772:SF0">
        <property type="entry name" value="10 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00166">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00297">
        <property type="entry name" value="CHAPERONIN10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00883">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2069103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="101" mass="10843" checksum="0DF83144B870DD02" modified="2022-05-25" version="1">MSSVKSFKPLFDRVLIQRLKNAEVKTSGGIFLPEKVTSKVNEAVVIEVGTGRRAGSGFAAPLLKKGDRVLVNELPAGEKIIVNGIECEVLSENEVLGFMGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVZ1</accession>
    <name>A0A8J4UVZ1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Amine oxidase</fullName>
            <ecNumber evidence="6">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_009503</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a secondary aliphatic amine + O2 + H2O = a primary amine + an aldehyde + H2O2</text>
            <dbReference type="Rhea" id="RHEA:26414"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:58855"/>
            <dbReference type="ChEBI" id="CHEBI:65296"/>
            <dbReference type="EC" id="1.4.3.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the flavin monoamine oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="6"/>
    <dbReference type="EMBL" id="AJWJ01000725">
        <property type="protein sequence ID" value="KAF2069180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVZ1"/>
    <dbReference type="OrthoDB" id="5046242at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097621">
        <property type="term" value="F:monoamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001613">
        <property type="entry name" value="Flavin_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050703">
        <property type="entry name" value="Flavin_MAO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43563">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43563:SF1">
        <property type="entry name" value="AMINE OXIDASE [FLAVIN-CONTAINING] B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00757">
        <property type="entry name" value="AMINEOXDASEF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="7">
        <location>
            <begin position="32"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="52"/>
            <end position="53"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="383"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="468"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601613-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2069180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="487" mass="54880" checksum="5034D21F971AF9BB" modified="2022-05-25" version="1">MNKYSNPMKENTHHSSLSIRDRYDCIIVGGGLSGLQAAKILSKKNLSILLLEARNRVGGRTKFVEYEGYKWDIGGQWLAEPESQYRMYRLLRETGQKTFPQTCDGKKVWILNEVANSFDEGCFPASIPENYRHQLEEAIEKLKILIDEIDIEFPWSHGRAKEFDSISLTDWLRNQCGCVEQTTEMICTIVMGILAKNPADTSFLYTLLYLKSANGWDSAFSVENGAQGHRIYGSSAQVSETLAKQVSEKPNVTMCLSQCVTSIDQSKDRKNDPFPSLIKVSFKSTEKEARSTTTTVYSKFLILAIPPLFINNIQITPSLPSKRIKLNQSMEMGNVIKFIIFYKNNFWKKMGFSGEIFSNRGSVNEAYDATLVDGRGGACIVGFFAGVNATKWANKSLVERRSEVMDIVHRSFGSNEAFQPINYIENNWMAEEWSKGGYTSVFPPNGVFSECGETLRKPFGNIHFAGTETATEWTGYMEGALQSSERE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UW73</accession>
    <name>A0A8J4UW73_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Long-chain-fatty-acid--CoA ligase</fullName>
            <ecNumber evidence="7 8">6.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_009279</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:15422"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.3" evidence="7 8"/>
    <dbReference type="EMBL" id="AJWJ01000669">
        <property type="protein sequence ID" value="KAF2069405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UW73"/>
    <dbReference type="OrthoDB" id="1700726at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd05927">
        <property type="entry name" value="LC-FACS_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045311">
        <property type="entry name" value="LC-FACS_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF109">
        <property type="entry name" value="FATTY ACYL-COA SYNTHETASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23562">
        <property type="entry name" value="AMP-binding_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="9">
        <location>
            <begin position="72"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU369030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2069405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="673" mass="75550" checksum="72EF3159C6358FAA" modified="2022-05-25" version="1">MIDNWLALGLVLVSGILCFNWKRKHPYGQSIEVGQKVEGEGRARRNALSPNHLVSHLEEDGIYTLYDSLIKSCEKYGDRKCFSERKKDANGNLGDFEWISYNTFLENCEYVAAGLCDIGIKPKSKVGIFSRNRLEWLTVHSASFLQSNIIVSFYETLGVESLAFVSEHAEIGVAFCSTETLEKTFEISRSVSILKTIICFDPVTDQQKEKAKKENITLYNYSDIIERGKKTMDKYPHTKPTADSLSTIMYTSGTTGDPKGVMITHKNLASVVCAVNNFIKVYESDIHYSYLPYAHILERVVVLTAFHYGAGIGIFSGDISNVLNEVKTLKPTLFIGVPRIFERIRAGVFREISKKSAFAQKLFHFAYGLKKFSIAKGFKLPMLESLFDSILFSKIKAQLGGNVRVILSGGAPLSRETELFLRVSFSCAVVQGYGLTESCGGTAVHLLEDESVGSLGPPFLSNEIKLVDVPELNYYSNDKSQCGEVCLRGPSISIGYYKDPEKTKSDFKDGWFYTGDIGKWNPDGSLAIIDRKKNIFKLAQGEYVAVEKIENVVGKSHYVAQVCVYGDSQKSCLIAIVHPHPEKAEEWASSKKSDKDLKDVCENQDFVKEVLDDITKTCKEQKLFGFEIPKAILLTHEAFSDQNNMLTPSFKIKRPQIKEHFQKDINHLYSKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWN6</accession>
    <name>A0A8J4UWN6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000963</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000021">
        <property type="protein sequence ID" value="KAF2077716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWN6"/>
    <dbReference type="OrthoDB" id="10264655at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20532">
        <property type="entry name" value="CYCLIN_CCNL_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20533">
        <property type="entry name" value="CYCLIN_CCNL_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000031">
        <property type="entry name" value="cyclin-L1-1-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036580">
        <property type="entry name" value="Cyclin_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0195">Cyclin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="2">
        <location>
            <begin position="34"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="2">
        <location>
            <begin position="150"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="254" mass="29923" checksum="8C6EF96FB9FF6D65" modified="2022-05-25" version="1">MNLYFSDDEILNSPSRRDGVSEQVEDNQRRYGAEILQEAGILLELPQSTIVTSQIIFQRFFSRQSFRQFDVKTIALGCLFVSCKFTNSLRKIRDLLNTFTHIWQKRENLPVEYIDTTKQHYWDLKGDVIAAEFEILREFGFMVSVDYPHKYILNYMKLLDKSTELAQKSWNYLNDSMRTTVAIQYRPEAIAAASIFLASRVLAEKLPEEPYPWWELFDTTRSEIDLISYEINNLYSKPKPFYIKFNNDDAVQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UX10</accession>
    <name>A0A8J4UX10_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008765</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000574">
        <property type="protein sequence ID" value="KAF2069913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UX10"/>
    <dbReference type="OrthoDB" id="20870at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039190">
        <property type="entry name" value="TTC14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23184">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23184:SF9">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="394"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="627"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="92"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="157"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="394"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="453"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="491"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="508"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="801" mass="93498" checksum="20FC51D7BA519421" modified="2022-05-25" version="1">MSSLSSNDVTMEVVEKQLIDLNLKDTQQEQLQQTDNNKEQPKAAAVKKVTPKKRIQPNLVSSVTTTNIQPNINVKYDLSESITSILNNIFTTNNNNNNNNNINTNTVSTSSNSTSNSASATTKIVEENTKSEKRVKQTKDTDTDRTTPTKPRRARKAISVTNSTSSTTSKKRNHDSEQQLQDEQQLQDEQHQQKEKQGKRKDKKEKKKKKIKVSKTLQLLEPFDFIVSSPPRLSSLFYLDLVEYFNDLANSSRGDDQESIKEYLKNKPFISSNLDLISKEYEIDKESLLNHINNIDNLSSIKYKLSIISDLFSISFENQNQHQLEYSKFLKLQKELWWVKTTNHWFVKPLKNTQPNLLKLWFGFLIVELWYRFVWARHTKTVYDQLLIEHNNSNGTSINNGISQDNHHSDDDEEEEEDVFDLQDLGEDDDDEGDEEEDLEEDDDDDDDDEEENKDKKESKDIQLDQIKNQKDESSEEEEEKENQMNTSESDEQKDCEIGDAVEKDGENCEDDEDDDEQEKSYLDKIPKVVSIFFVHSAITEAIHEIEIEYWETGLWNIKKVVDIFSQFIHSYYRDNKSLPNLTFAEIHENIHSIFNKKYQQFVYDLGVNISDNDSFIWYWNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNIDNSRILEFIDTIYVEQFKNLTQSGVVSHSHSFYFLFPYFQDMKPPTHPISDYYMSLKARLQKQGIYNKEVESIVETLDSLLEEKELIKSTLQKHQENTSGVFKSLIEIQQNRIKYLDQLISTMKSEYHKLTNKFDQLPSKKKKKSSTTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXD5</accession>
    <name>A0A8J4UXD5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000216</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000004">
        <property type="protein sequence ID" value="KAF2078466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXD5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="523" mass="60102" checksum="F81B49A4F5457644" modified="2022-05-25" version="1">MNSFYIIYRNQYLKRNIIKYIFEGSLLNVCLSRLNEYHEFLSLITNNTDNNNSTTYNNNNNISIRLIIKNHQEFKEYINHPYRYIVNTLHLGQLKNVSSFVNGGNKFDFGCIPESVTSLEFHISNDDIQGYGRFPQSITELKVNTNMFFEYEGNLLDYLIHHLPPRLARLKLPSNVQIREEWVVPESLVDLVCMFEASLCSNIVVAPTRVFKSSYVMINSIVELELLQSSKWMRSISIGAIHYPEQPHLFPPHIEEIVVYDHHDLHPVYLDKHMLPPCLTSLAVHQIDHPIARDTLPESLISLTIDVYKHPIRIGVMPVGLTILYISCQHPLEPGTLPLSLTHVNLYGFNHPLRANVLPEGLQELTLSAFKNILEPKSLPRSLTFLQLNKFKGSFESVGPLNQLVKLHIHTMDASISTVIANVKNLELLIKGPTIIHPSILLHSTSIEKLALYNHSRKLDRISLPPDFFPPTLIHLALFGFEIKSPNILPRDCIFFRHNIQDLDTNLLPPSVKYNKKEEYKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXK2</accession>
    <name>A0A8J4UXK2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008093</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000471">
        <property type="protein sequence ID" value="KAF2070588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXK2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="436" mass="50609" checksum="A4BD4E7BE8E187AB" modified="2022-05-25" version="1">MYIGILGPSNYYLKVVEVISRIYNPQLCINNSNTNRSNNNQEIKLNTKHLLIKIWNRRYLQFFINYYENNQLGPIRSQAIILVFSVDDRDSFNQVAHFFQQVDKYEYGGTPVILVGLSNNHTDVGTDNNRVGLSEITNLYPRSIYCELKTFDSPENIQDFKKQFHPLLSLMFLSCPSTISQICSKTAPYKVKKAPDLFDVYPYSTPVDFSNQTDRLAYEQSFFKVFKNKVILSYIFKQIHHINHKYQIKTFNPFTATPNVLIANGCINQLKDRLYNSNNNNEIEFSYLDAVCLLRSNDTDFQLFKFVYDRAKHSFHSEKGDLYWTASQGTLTNLHLNTIKCLTPIENASIGGNIDIVKFLILEREKKEALDISLDLAILFNHLPLVKFYLKIMKGYCFFDHLIALANSQNNETIKQYLYKKSKSINLNKIKSLVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UY44</accession>
    <name>A0A8J4UY44_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006635</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000315">
        <property type="protein sequence ID" value="KAF2072045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UY44"/>
    <dbReference type="OrthoDB" id="18391at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="283"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="391"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="434"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="476"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="538" mass="60965" checksum="532248E4C60D3947" modified="2022-05-25" version="1">MINNNNNEIPLEEFSPPSLGRSDQSVGSSGSSISGDLESQGGLNSTLDKSIGTSLGTSLEISTDKEQKIQDEENFDKDADILKNKIGWRGLKTVRLMMERYLYGIIVAVVVSLFIVILSLPNWSGAEAHYLNIKTYSIFFSFAILLFGPIYVTLSFLVSYGLENVKKSKILMYTVAASIVFCILFAVLYLKGVKYWFYYLDALAVLVVYCLGEVICAYRISGVLQKEENPVQKINVVKALTLAYNYAAPDICVTVFTLIYMFFLIPIYLTINSSILRLGWRLIIHPAYWTLIVMIARQFLTKDICTEDIMRNSNIVLHTFFHHQTLGKIFVYTFSDDGALTEIGMVISAIEDIILRSLALKRDEWFFTLLIGREKARAHVYSQQSLQLRAAILNIQVSLEFSGLITAPLFVYMFQKHRVVFHFFDGPNIAPVTLLYQSILSISLDVMAEFICTYIETRFYKLPIYHTWRWMKSNKGFLCWIVYGSLSMGLLAMIWTCARVPRAGLCTSDDICTCSSIIASSIPEWCYNSNSTLSTMTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYD8</accession>
    <name>A0A8J4UYD8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006919</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000340">
        <property type="protein sequence ID" value="KAF2071773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYD8"/>
    <dbReference type="OrthoDB" id="23622at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="307"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="340"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="554" mass="63017" checksum="A86947457C87E79E" modified="2022-05-25" version="1">MGTIVAYLRGVLKSYEKLPGVLDKRIYQIRLLFKDDAPENKALGITEDDYEDYRCLLSHRKLSGILVISSLLEIMLTLINYFIIHGNFEIHRDIGNVLETFGVMVITQMIIFKPLWTAKLMAAMSTLILTTNLLPKILNIHSLSYNYDPTLQVMLWVLFYYPTGNFARSSIIGIATVVILIVFRLVHKNYSVVNILFVEGASYLAYAVVNSISQRSNEFFQRSLYVELKQCNPDLPPHKVWGIFNTFYQQNSFKYLLFFNTTEMEHRFLGYYSKQCNLELGILLSTIISSLYYLVQDFQLHKTTDVAVFIALRFAVIIPCSLFLVYCSFMRRQNPYRADFLSSVSVLCIVASQIIMFVKTGPNESSLFYLGGVMRALTFLSSQLLLTPLVFPLMASYVMIPIAAIYYLSGTFTHILFFTMFTSLAFDFSFNREKTQRIRFLIRYYKNQALSEKGKGGGEDVESKLTESMIQPPHPTILPVLASIPSQILSNPKPIPPTPVPLAMSLPANLSNLPKISDDDDIELDTFNSHSLVDPPVIPLSSSVDSIKKFNEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZI8</accession>
    <name>A0A8J4UZI8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005564</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000225">
        <property type="protein sequence ID" value="KAF2073123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZI8"/>
    <dbReference type="OrthoDB" id="24384at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32142:SF64">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32142">
        <property type="entry name" value="B BOX-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="452" mass="53577" checksum="4DD92646F052F3B4" modified="2022-05-25" version="1">MYILTITGDSDEGIKSIINVFERKCEVKVKEENYNFNRYLYKFKTWNQFNPLFQNWGQDVMRYSRDGTPIVVIGFYQDPKDPKNISREHYHSVYPFPYYEFQITNISEQDQFNFDKLIMEIVDYFYLSYFPTHFYDQKVEKQVISKKNNNSYPITSTTIPKSTTTTTTTTTTTTTTTTVANPKHAFFFGTSKPGNEKEKGEDDIENLPIAFINNNLSSSKQSHYEAMFFRVFRNKLLFKLIFKQIHWIHQRYNITAYSFYSSPFSLFIQHRRLDLVKQRLELQNKGSNRLHVWDQFGYMNVINFLRVNNNYQLFQIVYDLFQRSFTLDMLLYFVDYDESSDFIDRGPQILESAVIGGNIEIVKFLVSKKFECNERALQFAVVLGHLDILKYIIKEKVFIVPPALMKSLLELSTRYNHKKVEDYLVKVQTLSTLSLAPPPNRSFIKKFIRKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0Z9</accession>
    <name>A0A8J4V0Z9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_002178</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probable adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000058">
        <property type="protein sequence ID" value="KAF2076500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0Z9"/>
    <dbReference type="OrthoDB" id="5989761at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001293">
        <property type="entry name" value="Znf_TRAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131">
        <property type="entry name" value="TNF RECEPTOR ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131:SF94">
        <property type="entry name" value="TNF RECEPTOR-ASSOCIATED FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02176">
        <property type="entry name" value="zf-TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50145">
        <property type="entry name" value="ZF_TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="129"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="TRAF-type" evidence="10">
        <location>
            <begin position="276"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TRAF-type" evidence="8">
        <location>
            <begin position="276"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2076500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="459" mass="53724" checksum="36CA1B6201E397EE" modified="2022-05-25" version="1">MFGSSLKKDEFKFDKLLFGVVEKSESFVVYCNQDETCLSFKHKIAKVLDVGCDDSFLFWINQSEEKLDDNQLISTLSEHSCIEVQHTSYHTPRFLERSAEYLDLSFTYSNLNYDLGSILCEESKSLYICNICMEYVTKQVQCVNGHLICGVCANSIILKKGTCPTCRATLSKDILSRSLFYEQILDRENVTCPYYLEYTLKNKDQESKENPKPCDQIKYKVLVSTHKNECEFRMIKCVCSKEMMYYEYQRHKQLCPKSKYKCRYCYEAFNKEDQETHFSRDCKLIPIPCWQCGTKTNRFELNSHTEKDCLNTLIPCPYAELENGCKDLVKRRDLAAHLLEYDSHLKFVLSISASIKSLKQRIDNVLCSYYVTKQDCNPNQDWSIPNFKGNFKITGPFFEITSPFDLPHGAYEFVLSLYDKDYNLFRTFEEKLKPSRNFLNVKAWDTVHSLKFTIMKSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V156</accession>
    <name>A0A8J4V156_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MACPF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_008557</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000538">
        <property type="protein sequence ID" value="KAF2070127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V156"/>
    <dbReference type="OrthoDB" id="21110at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742">
        <property type="entry name" value="COMPLEMENT COMPONENT C6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742:SF8">
        <property type="entry name" value="FLOCCULATION PROTEIN FLO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0204">Cytolysis</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035166880" description="MACPF domain-containing protein" evidence="6">
        <location>
            <begin position="19"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="domain" description="MACPF" evidence="7">
        <location>
            <begin position="481"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="726"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="728"/>
            <end position="748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2070127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1080" mass="120811" checksum="C9F4EB951111D6E6" modified="2022-05-25" version="1">MNGYIIFVILLYLSVSLSQQQFSLDWGSTCKSGCGSFNKPFSSFEEAISELSLTKFDKTPVLLVNKGVYFGENNKAIIINFSIDIIAISSNRDETVIDCEDIDFGFKVLGSFTKFSIQGVTIKNCIAPRGAALLTENYFTKIDNVVFRGNKARQGSAIFSSSSNILIKDSYFLNNSNDAVEMENGFGQVIKSFFYGNEKDIVCTNSSVSNINSEFGSTCTYCTILNENRDNICQSRKRQDLCNSNGECDEYIEDYQNCPSDCQYYDQVEALIEASDIGHPGWKLQSFKESLPKPINSTSSSPTAAETLPQVDYLEYAKVSNFMGQHSSQVSGILSSFIQVTKSGDYSFRLKQKNLIAIMFVNSRVLFDNFFEENVKQVTQERRILLSNSQPHHIIIHFNGVNDMERDLELEFRYGDHGPYTLVPSIFITIAPNMSCGDDICNEDPTSCLIDCYDHIEKDCPAQSPPADIQSYYGPIRDTLGALLNNQYIFSLPGLNYLSHGMNIQTHEISPSPVFSITYCDNTSFSLVHSPSRGLAYSVPPGIYAQMSPKCTMDTTTKTYSTSSQMAYESSLEMDLDVSASGSVGGWLGHLSVSASLSLSESTQKAGETQGKKDGSISKTELKCQVSKAHVEEPKFHTKFIQDIGNSNGVDKASILTKFRKLIQKYGSIYFKSITLGGKLEQMSVVEHSFSSSKSSSEVERSMEMSFAVQASSKILPVGGSVSLSGSMDSKTSSTEQNQFENNSSRSTLTVYGGEPGSYGDEKEPNSFAKWASTVDQIPYPIDYQVGFIADVIPKEWILKNGFNVQELWREAEFQLYKDLFIAAKTSKYLDEPMIHNMGRNHTVYLVEYDKQSCQFTTFKLEVESPTGLFHYNFTLSQSTGATILPLEYDDRKGIKSLSFSEYYSPFTCQTIKMFNLVTSRSYHFVPDPTVPNKYILKQPEPNSVIVEFQGTNNVNTEFITEIQFIGTTGVVKKIVLNFSEKLSFVIDYFGDLVGLSFTFITNYENQNLKKDFIFTFSSIRVTQACPYAEKSSPCILKNMDFIPSDGFAKTFEVWSRESDKITLTYSNKPHWVSITPIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V194</accession>
    <name>A0A8J4V194_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_003412</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000106">
        <property type="protein sequence ID" value="KAF2075283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V194"/>
    <dbReference type="OrthoDB" id="19141at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02800">
        <property type="entry name" value="tRNA_bind_EcMetRS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004495">
        <property type="entry name" value="Met-tRNA-synth_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051270">
        <property type="entry name" value="Tyrosine-tRNA_ligase_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586">
        <property type="entry name" value="TRNA-AMINOACYLATION COFACTOR ARC1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586:SF37">
        <property type="entry name" value="TRNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="4">
        <location>
            <begin position="48"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2075283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="192" mass="20542" checksum="4628981F3E094343" modified="2022-05-25" version="1">MINNLLKSSSTLLTRSLQGTTSRYYGAACCGGKKPIIPAAPSEIELDDFLKIDLRVAKVIQAEHVDGAKKLLKLTLDVGIKQTPPAPTPSEPLPSSESTSTVVEATTEATTNATATTTEPIRDIRTVFSGIKAYYSPEQLTGKNVIYISNLKPRTMKFGVSQGMVLCASDEKGEKVLYTTTDDGSVPGMKVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1G3</accession>
    <name>A0A8J4V1G3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010560</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001195">
        <property type="protein sequence ID" value="KAF2068114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1G3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="498" mass="57409" checksum="6BF55ED7E93AEEE5" modified="2022-05-25" version="1" fragment="single">HLITHIIVSSHFYVNHYDIVQQQPQHQQPILFGLSITTPRDPLILATELPLSIKYFKYTTVSQQLTKEYIPQTITSLYLDISVRIDRIPKSVKTLTIDSTYQSKRDKKRLKGLRFHHLSQLLPPSLTKFQFNDHYYNAKIDFNLNQMPPNLTYLAIYGDFTFTGNGNGSLKYLKLNTGSDGPYLIPTSVTQLKMSTPSLDSTPLPPNLNHLEYNFIGLVGETATSPKKFPEKLTTLSLKNCSYTNISKNNWPSGVLDYRNWSEQSEKDVPYLPPSLTKARLCIDKDMKELPILPSSLTELKMLSTRRFETFTGPSPFHSYFPNTIVKLVLQYSGELFIGLIPTSVTDLKLNYPKPIPKGAIPNSVTKLHLVNNSYLVQPIVIPNSVTYLTFEFARQLYPELEIPDSVKTLKIIYLEFNNTPLLDFFKTPFKNITRFSTYEFMHDRLVEKLRDKKLFPNLKQINVVGHYNHIIEQNMVYNEVDYRNNRGGSSNFKNKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V258</accession>
    <name>A0A8J4V258_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FAM91 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_007512</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM91 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000405">
        <property type="protein sequence ID" value="KAF2071177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V258"/>
    <dbReference type="OrthoDB" id="275996at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039199">
        <property type="entry name" value="FAM91"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028097">
        <property type="entry name" value="FAM91_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028091">
        <property type="entry name" value="FAM91_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28441">
        <property type="entry name" value="PROTEIN FAM91A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28441:SF2">
        <property type="entry name" value="PROTEIN FAM91A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14648">
        <property type="entry name" value="FAM91_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14647">
        <property type="entry name" value="FAM91_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM91 N-terminal" evidence="3">
        <location>
            <begin position="13"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="FAM91 C-terminal" evidence="4">
        <location>
            <begin position="369"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="domain" description="FAM91 C-terminal" evidence="4">
        <location>
            <begin position="693"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="332"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2071177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="795" mass="90882" checksum="1E8936D427C91A0D" modified="2022-05-25" version="1">MDQHSEKELEKYVLNQVTWDSLPSHAKSILDQSQTKWKQYVLKYSIKHQLRWDSSLIRHYVTDERIYYQEIVRLSVANLVLYPYHISNQLVRMLNVTPFKYYLIMMIETMTNSKSYDELPNFTAVDCIRVLEIGRNQYIDLMNKCRSKGFLFKKKRDVIKGLLPTKSLEKNTDYWWIIKYGYHNNDEERSCTPQEMEVLEDLKKSTNDQGKQAGCYPMDVVLQLVSKGLVYIDVPIQNSDMISVPPLEGFVMNRVLGDYFENLLYKIFVSIDERTTVQELSEVLQIDVELVKQAVSLYIRLGFAKKKNIEPLQQTLDDQVVSKFHSSWINSSVGSGSKEENQSLLNNNSNNNNNSSDSNINELEDNDLQRIGFIFDSSITAFLMMGNLGYGLKNHAVTMFEVGKLANEALCDFLNELDKIDIEEFDTEAKSYAYNAIALRNTIRHLRNRYVIGGQMQGLDLISCERMNSLEENTRLRVLKKNYSVLVSLAPLAIDNCPVISCIPVHFGPPVYEFHSFWFRLFLYALIGQGPDSILFPKGSRLKRIPQVFRDCEKITICSIDHDPVTVNLSQLLPTVNEILLSSPVLLQVYSYIKYDMAPKQSKLMSHSRSTSTGGTLKEEKLIYNIPFPLDKPSLFLNQEEYNESNIDQHPVLMKIQDCLKLNHSFGYISLLKIELPSTSGNNSSSGGSTSKPVIKWLPLNVYYGIPIFDSDLNQSVCDNIDKFGLLTEDNIKQQSFNSRSLSLDLFNFISSTCPPIHPDLDVIDSTKGHASLPSVQNIPLPTQTISFINGLLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2F2</accession>
    <name>A0A8J4V2F2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carbohydrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007215</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000372">
        <property type="protein sequence ID" value="KAF2071462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2F2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019028">
        <property type="entry name" value="CBM_49"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052879">
        <property type="entry name" value="Dd_Spore_Germination_Stalk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33239">
        <property type="entry name" value="CELLULOSE-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09478">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01063">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035271371" description="Carbohydrate binding domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate binding" evidence="2">
        <location>
            <begin position="190"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="287" mass="31959" checksum="C19B2DF9761B8890" modified="2022-05-25" version="1">MKISILIASVFLCVALVRGGTETHSNNQYGIHQDENGGYRIQNGPTYPTQNNNGYYVQNGNTYNPNQYYPTQNGQSYYPSQAYYPTNGNNGQVIQNGHTYIPTNGQVIQNGQSYYPTNNNNNNNNYNTPVNYPTNNNGQVIQNGHTYIPTNNNNNNNYNTPVNYPTNNNGQVIQNGQMNTKSTTNCVSQVDISVEMNNTFVENGQTYTNIRVTIKNHSEKIVTNLELCTLQYEIKDSKSVWNMQRVSSNVLTLPHGTSINGNNSHTFGCSIKGKQIPTISINNAICA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2T3</accession>
    <name>A0A8J4V2T3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_006776</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000328">
        <property type="protein sequence ID" value="KAF2071897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2T3"/>
    <dbReference type="OrthoDB" id="13376at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097178">
        <property type="term" value="P:ruffle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000178">
        <property type="entry name" value="Calmodulin-2 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049025">
        <property type="entry name" value="AIF-1_EF_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042433">
        <property type="entry name" value="AIF1/AIF1L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10356">
        <property type="entry name" value="ALLOGRAFT INFLAMMATORY FACTOR-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10356:SF0">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21008">
        <property type="entry name" value="AIF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="38"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="74"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2071897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="140" mass="15817" checksum="132D27C9E6BEB065" modified="2022-05-25" version="1">MASTKNYQGGKAFGALKKQQEATLDQICDEFRAELDEATISKYKAQFISYDVNNSGDIDLYELQLMMEKIGQTKTHLELKKMIQEVDSTGTGTINFRDFLIMMTGKKSSILQKILMFEEMAKKPETPKGLPPKRSIADLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3E2</accession>
    <name>A0A8J4V3E2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_001942</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000050">
        <property type="protein sequence ID" value="KAF2076753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3E2"/>
    <dbReference type="OrthoDB" id="278325at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1230.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1230.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001931">
        <property type="entry name" value="Ribosomal_eS21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018279">
        <property type="entry name" value="Ribosomal_eS21_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038579">
        <property type="entry name" value="Ribosomal_eS21_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10442">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01249">
        <property type="entry name" value="Ribosomal_S21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002148">
        <property type="entry name" value="Ribosomal_S21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00996">
        <property type="entry name" value="RIBOSOMAL_S21E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2076753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="81" mass="9015" checksum="1D881DD8378A0E24" modified="2022-05-25" version="1">MQNDDKKFTDLYVPRKCSSTNRLITAKDHASVQINIGHVNEEGIYTGDQTTFAFCGFLRNNGQSDAALNRLAQGKGFLKSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3H8</accession>
    <name>A0A8J4V3H8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001856</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000047">
        <property type="protein sequence ID" value="KAF2076828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3H8"/>
    <dbReference type="OrthoDB" id="6359816at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="483"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="636" mass="75013" checksum="2BE37060D217BA3C" modified="2022-05-25" version="1">MRRLRTLRPYNTNSNSNNNHNNDEENVNNFKIITPENYDTINYFKLNDNIFLLNNNYQESSLEYQTTLDELVKQRRLFSTRLVYNSCEELYSISVPYHNSKDINTKITPSQDLLQPNKFSNNLNNTIIESKHILSKVAEYKKVNVITRREDHFRMYYLQCSHSGKGYIHWKWNFDESLTISKLLIVLNYALFNFSCSIDWFVSTEKDIEFNQNPDSWKRIPLYRTGTKIYIEKQLDLTPFVKDCNSFHLVVLLDSSNSHSEHAQIFRKSSNQSENNPNPIPISKFDFPFSIDIDLSPKYNSTLNLQKDNYNNMDIQQENNNNNNNNSNGKILNSDLFMKQIYEKVKLKTKSYNDIDPQQWDTNRVCQWLFDNGFYQLIDFFTNEKKTGKDIFTLEKNLIPKDIKKKWKASIQSFNLYLGKPVKNLNRKLYHMCLNDPYDSNSLLRIYKTKSCESDQDSNGNSLVVRFPTPEEFPLILNDKLSSDYQIFVDDQLIYVHKAMLCKWSEYFSALLLSSSHFSESLTNQSKFKSEDLDYNSLLQVLGIMYCENEPQIIETVKQLNISSLFDLFMTSRSLMIDLLVEVFEKVLIWKVDQNNYKDLLELAEQAQATNLFHFCTIFSKLPFISSEESYQHHEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3Y8</accession>
    <name>A0A8J4V3Y8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001635</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000040">
        <property type="protein sequence ID" value="KAF2077068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3Y8"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051709">
        <property type="entry name" value="Ub-ligase/GTPase-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45622:SF70">
        <property type="entry name" value="SECRETION-REGULATING GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45622">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="39"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="RCC1-like" evidence="3">
        <location>
            <begin position="41"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="97"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="146"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="198"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="274"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="327"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="382"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="433" mass="47011" checksum="1E06327C6162250F" modified="2022-05-25" version="1">MLNHKKIVGGVIGYVNVVACRGGYTTSRYYSFANNRTKQQLYAFGSNENLKLGVPGIEDRNVPTQITNLDPFINIKSFGSSWLHSYLLSKDETNDSTKVYMWGNNRSGNMGLPAESYKYPVLVDILKNIAKISIGRSFSLALTNDGKLLSMGENSFGQLGLGHSLNTPTPTLIQDLASQEIVDISTGLDHCVAVTKSGHVFAWGYNLEGQLGQKIVEYQTTTSTAIPVDEKEAFSNFEPDVEYNTPTLVPGLESIKIAKVFCGFDNTLLLSARGNVYGFGSNDVGSLGLGSELKGRLMKPTKIPMGNEKVQSLSSGPSHTMFVTNQNNVYVCGWGREGRLGLGDNTSDRDTPTLIEYFKENNIKIVKVAAGGSHSLALSQDNQVYSWGNGANGKLGHGNETEQKIPKLIESLKNKKAIDIAAGIDSSFVLVEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4T9</accession>
    <name>A0A8J4V4T9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NlpC/P60 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004795</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000176">
        <property type="protein sequence ID" value="KAF2073907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4T9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051794">
        <property type="entry name" value="PG_Endopeptidase_C40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47359:SF3">
        <property type="entry name" value="NLP_P60 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47359">
        <property type="entry name" value="PEPTIDOGLYCAN DL-ENDOPEPTIDASE CWLO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035285910" description="NlpC/P60 domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="25904" checksum="5846869658768E09" modified="2022-05-25" version="1">MKTTTLIFYIFALVTVPIINAITVSESINKLLQVKRSVPTQPLSVCITAAAIAKTHKYCNCWEVQNCPCNANVLDPLGLITKSFAKSGLDIFSKSLPACGTKMTPGTYVGSDSNACNTWKVEIPISGSTSGGQGDPNIPSFNGNEEISTGNGISQTLIPKTCDPSNISNCQVGDLFFYCATGSDVCPSHVAMNVGDNLIAECPVNSYQCNVREPYSENSWVVVQFVSQNKKFEIFLTYEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5I4</accession>
    <name>A0A8J4V5I4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004113</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000140">
        <property type="protein sequence ID" value="KAF2074567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5I4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="273" mass="31060" checksum="3D05DF89453FEEE9" modified="2022-05-25" version="1">MPIHNTTISYFADYCDLCSNKRIKSLAESAINLLKQKSAIDSLSHLHPPRQHSTAVRALMKDKLNNWLQEFNFFDYDTIYEPMDQVLDISFSISSLGSDQDDDHSSLFQISTNSTVSTTSTYVAEQKSLVGLVDNQDSSNNGPSVISSQILNTFMDENEDDFDDRHEALPIGDSSVYNFDDVDFILDIPSDDDEVSLIGKETVLYNYNNEQYLVVHSDEEYEDGESISSSFLDSFSSSSSCLPVDNRYEVNIAQYSVLVIMFIIWIFLWFRNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V612</accession>
    <name>A0A8J4V612_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000029</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000001">
        <property type="protein sequence ID" value="KAF2078658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V612"/>
    <dbReference type="OrthoDB" id="1913277at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="18313" checksum="B142204BE264C757" modified="2022-05-25" version="1">MTQGDETSSGKRILASTFLGGIAGTLYGVHKTFPESVFSDNTEKYLRDSRKVFKRAIGGGAAVGFTYQATKQFVVDLRQSNENDKVAIAAGVTASALLGGIASSSFKNGFAIFFVGVPLAIGISVLGSEYAESEKLIQSRFDTQGKTKLVLHNKVEELRREQEALSKPSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V626</accession>
    <name>A0A8J4V626_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003286</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000101">
        <property type="protein sequence ID" value="KAF2075407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V626"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051948">
        <property type="entry name" value="Hsp70_co-chaperone_J-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="14"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="263"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="280"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="290"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="407" mass="47351" checksum="295E1C1EAF90CD10" modified="2022-05-25" version="1">MDDLTFLNVNPYPKLYEVLGVPTDASPTAIKEAYKRLSLENHPDRGGTSERMSEINEAWDVLSDFDKRFVYDNIGTTYNIDQMKNASRVPRFIMNHFAVPLITFLDKSLGITDVIFTYTFNSTYFKLSPCHVYGFRVIQYTVDSILNNQLNRLFPKNIRLNTIVSDIVSTTVAAPFQIASYYAFLNRANVSGFSEYFLFDYPKDIRSVPAVLKFFYNFPQYIKYFSKSYKLIAGIVLFRFAYTFSHYFLSSISDYAEHRLLQNQSEQQEEQNENERKKNTGFSRYYGQQSNNDNDNNDNSSNDNDKQEKREQNSLKWFVVNRVFRILSFLVPSTLFSVHRLFYVLEGRGNYFDTSLIRAGVVDAFFNWGSDYTVSVIQQQVRTFGTILKAIIHNVRSIVVSNTNKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V680</accession>
    <name>A0A8J4V680_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sphingomyelin phosphodiesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_006156</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Converts sphingomyelin to ceramide.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the acid sphingomyelinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000271">
        <property type="protein sequence ID" value="KAF2072539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V680"/>
    <dbReference type="OrthoDB" id="282973at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004767">
        <property type="term" value="F:sphingomyelin phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006685">
        <property type="term" value="P:sphingomyelin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00842">
        <property type="entry name" value="MPP_ASMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000077">
        <property type="entry name" value="Sphingomyelin phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.225.10">
        <property type="entry name" value="Saposin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045473">
        <property type="entry name" value="ASM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041805">
        <property type="entry name" value="ASMase/PPN1_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007856">
        <property type="entry name" value="SapB_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011001">
        <property type="entry name" value="Saposin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008139">
        <property type="entry name" value="SaposinB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011160">
        <property type="entry name" value="Sphingomy_PDE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340:SF34">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19272">
        <property type="entry name" value="ASMase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05184">
        <property type="entry name" value="SapB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000948">
        <property type="entry name" value="Sphingomy_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00741">
        <property type="entry name" value="SapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47862">
        <property type="entry name" value="Saposin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50015">
        <property type="entry name" value="SAP_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035274254" description="Sphingomyelin phosphodiesterase" evidence="14">
        <location>
            <begin position="23"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="15">
        <location>
            <begin position="60"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="392"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="428"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="64"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="67"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="95"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="193"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="352"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="554"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000948-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000948-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2072539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="608" mass="69153" checksum="2E0A7A40C74F2E50" modified="2022-05-25" version="1">MIYSRKYFGIIVLLFFIQSAFSISQHHDYQIPIKVANDRLIDEHGNINAESIFNSDLVGLSITCDICKFGIKELESMIQTKKDDNEIMDFAVDLCTLLRIEKPEVCQGLLPLYRNETFQVLGNPLCTPEFVCGFVGLCPYNPTNFTGIVHFPKPKPPHVDPVPPAKGNPVKRILHLSDVHVDTLYVEGMNSDCGEPICCRAANGPGSGEKAAGKWGSYTCDVNMLTLNSMFEFIDNEIGDIDILFWTGDNPPHDIWMQTFETQLNASLRITNLLKQYFPKAQIFPSIGNHESLPVNSFPLPPGSSWLFDALSQDWTNWLGEEAALETLRYGGYYTLPLQEGIRVISLNMNWCNNENIWLIENVTDAADMLQWTIQVLQSAEDIGEKVYIVGHIPPGILDCIDLWSQQFYQIVNRYEDTIISLFFGHTHRDQFEVFYTNDTSYSTGQRPSAVAYVTPSVTTFQYQNPSFRIYSVDAKSGQLVETSTYHLDILDANQYDKPNWVLEYNTTVAYSMPDLLPISWDNLIDRFGRNQTLFNTYYQHVYSSSPYPQQKPCGDSCRLDTICQMKAGTHSVYKECLHTQKSNFLTKSPIELLIELIDHVYTSLKSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6F2</accession>
    <name>A0A8J4V6F2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA polymerase II subunit A C-terminal domain phosphatase SSU72</fullName>
            <shortName evidence="9">CTD phosphatase SSU72</shortName>
            <ecNumber evidence="9">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_005901</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Protein phosphatase that catalyzes the dephosphorylation of the C-terminal domain of RNA polymerase II. Plays a role in RNA processing and termination.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 9">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SSU72 phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="9"/>
    <dbReference type="EMBL" id="AJWJ01000248">
        <property type="protein sequence ID" value="KAF2072779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6F2"/>
    <dbReference type="OrthoDB" id="57957at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006811">
        <property type="entry name" value="RNA_pol_II_suA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20383">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT A C-TERMINAL DOMAIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04722">
        <property type="entry name" value="Ssu72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2072779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="212" mass="24295" checksum="FC6C295D5F7156F9" modified="2022-05-25" version="1">MSSNNNNSFNNSNGIKRLAMVCASNQNRSLEAHNLFNNKGVKGVRSFGTSAHCKLPGPSIDKPNVFPFGTAYQSIYDSLKHQNVDLYTQNGILNMLERNIRVKLAPERWQEEVKQKFDLVFTFDQRVYDMVIDDLAQRDIGGTGLIQPIHVINLQVKDTHEEAVGGALHAYDIYTTLSKFDDWDDSKLDWDEVLDQFYKKTGRQLLHCLMFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6R9</accession>
    <name>A0A8J4V6R9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit C</fullName>
            <shortName evidence="4">eIF3c</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002574</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eIF-3 subunit C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000072">
        <property type="protein sequence ID" value="KAF2076112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6R9"/>
    <dbReference type="OrthoDB" id="29647at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03002">
        <property type="entry name" value="eIF3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027516">
        <property type="entry name" value="EIF3C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008905">
        <property type="entry name" value="EIF3C_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058999">
        <property type="entry name" value="EIF3CL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13937">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITATION FACTOR 3, SUBUNIT 8 EIF3S8 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13937:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05470">
        <property type="entry name" value="eIF-3c_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26569">
        <property type="entry name" value="EIF3CL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="6">
        <location>
            <begin position="611"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="156"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="820"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="156"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="173"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="210"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="228"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="262"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="827"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="856"/>
            <end position="883"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2076112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="899" mass="103199" checksum="0D8B293BEE92C048" modified="2022-05-25" version="1">MSRFYRTGSSSESESDSSSDSDVQVKKKTVYQSSSEEEIEEKRVVRSAKDKAWLQFDDILKKLRNALKTNDWVVISNEFDNMTKLVTNNKVSKIFEKEGFPPSFLKALYLIQVDHRDMTAEQKKKLHANNNKSYNSMKQKLKKTCDLYATQLKPYHDNPELVKEEAPAKDDFSDSESESESESDSDAPSNKKKPAPKKPQSSFFKKGSDSESESEDDGLESESWPSESDSDSDSDSDSDDGGNKWLVKDVKQTKTKATQTVIERKKDEDKITAKKAAASSPTVGSPATAAGQEKLTNDEIMQKVKEIMQSRGKVKTETSKQIQQLESLVEQVTGDKETFIVLYQLVATQFDTASVKVALTIPMWKNIATGIDKLLKLLEKNKHFVLAIEHKEPELEKGQVIVYGNLLSLFEMLDDEFNKSLQAINYPTKEYLDRLADEPILLELGTNLQEFYENSKNMEAAARIAFRRIEHSYYKTNTANPTEQSQLLSKLSTFIYQFGDERLNARTILCNIYYNAINNRFHEARDMMLMSHLQDNPTHMDVSTQILFNRSMVQVGLCAFRCGYINEAQSCVADFSGLRKDLLAQGLSIQSKTAEKDPIREVEETSRILPAHMWIPIDLIETVNLITGMLIAVPQNAFRPFDPKFKTCKFYQRHMDSVDRQIFIAPSETSKDIIYQASKALSAGDWTLTVEHIATLKFWSSIPDADQVRERLNKIVQQVCLKTYLFTYSVYFDTMLISELADRFQLQSSQVHSIVSKMMTNHEISASLEHSTETITFHRAEQTKLQYLALHYSESLVDFVEQNERIYDVKFGTTFRKKDEMTPQQHHGGHHHQHHQNQHHHNQNQHQQKQHHQKSHQQNQYQQKQQSQQQQSQQQQQQQQSSQPKTNKKSNRSGLVVNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6S4</accession>
    <name>A0A8J4V6S4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Right handed beta helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_002589</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000072">
        <property type="protein sequence ID" value="KAF2076127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6S4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035322189" description="Right handed beta helix domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="548"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="472"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="473"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2076127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="617" mass="66625" checksum="AE79DB54D70977BA" modified="2022-05-25" version="1">MRLFVLILVIVQLLYGVSSIETQQAYDTSTVLYVSPSSYYDAFNSCGYNIVTACNTIFDAFYVFSNSSMVTNSDPYKIYPPLILMLLPGTHTSQSFSKVHIPISGFNISIVAYNNEKTVISGKDRPAINQGMFSFTQKNSRALNSTAILSIGNVSVQDFTTMFIYVNIIPQTVTLNMAGTTFTNNKTPNGLISVSGYTYSGNKPNIKLTNVVVTKNTGTFLYSLSSIVSGAGLKVASNEELNAIISYSSNTTIFNSTFTGNVAGDLVDGIVRQYGGSLYISSSLFQKNAIGSLVYAYSSNNVYVAIESSKFIENNATREIGNVYVGQGGLIVTDCEFIGNNALNGGAISCNGTGTTISNSQFNNNFAENGMVLYQLQGEVKLLDSTVIYNNTHKHNVYASNLLWVYGGITHIKTSSLSIVQEPGDLLEGFSMIRCISSYVQIAPNSFVASEYAPVSCSACVLLDSPNYSCGGSSESSSDYDSSSSDYDSSSDYDSSSSDYDSNSSDSSSYSSSSTDSSSSLRPNSTSSSSSQSSSQSSSQSSSYVSPWTPYLILLGLLIFFCGLLFISILIIIICCVKQRRDEKKHREQPIINERTRMINSGSDSVIVVHQQAIAID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8A6</accession>
    <name>A0A8J4V8A6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_001090</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probable methyltransferase.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000024">
        <property type="protein sequence ID" value="KAF2077627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8A6"/>
    <dbReference type="OrthoDB" id="20143at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050869">
        <property type="entry name" value="H3K4_H4K5_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197:SF251">
        <property type="entry name" value="EG:BACR7C10.4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="6">
        <location>
            <begin position="29"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="MYND-type" evidence="7">
        <location>
            <begin position="71"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2077627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="488" mass="56951" checksum="5C0172332506568B" modified="2022-05-25" version="1">MSREIEKQQELDRREKTLQRLIADYGLKGKVGMKKSPIHGVGMFAEKDYKVGDIVVALKPYSYISQLDVRCDGCFLLFPMKLSKCSGCNFINYCSKECQQKMWPLHKLECASIKEYINCFKQQERVEENLLHILLIARTINIATLGCQNGGSLALIHNLCDPTEDLDVEENKLIVKKYLEFAQQVRNFLKDSKKPESEFDALVIKIFSNYTQDNETMTLTPILSLFNHSCNGNTILTSPNNMMYLLAQKEIKKGEEITYPYVYSDNKRVRYEILKSRGAICTCQVCDSVVKTGSQDTYYRCHGCNQRVDIRYRSTKDHTMVGRCLGCQKEYSASELKHKGDVIKSNIAERFKSAPVEIKKLFLTLYFEHFHPLHLYLIYLDFKDIYSLDDPNIFKRIELQVLHHYGPICDLALHYFGKIILEREKENRKDRIYAHFINLRRPAREVFKNIFLSPIFWDNNLEELKKKGIYNSSNILTEPDKYFDKTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8B1</accession>
    <name>A0A8J4V8B1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001029</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000023">
        <property type="protein sequence ID" value="KAF2077642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8B1"/>
    <dbReference type="OrthoDB" id="26371at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF457">
        <property type="entry name" value="RABGAP_TBC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="544"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="78"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="599"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="599"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="627"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="638" mass="73529" checksum="DB67041A3923E04A" modified="2022-05-25" version="1">MALAFPPHAIQAILETEHNLFNNKIFLSNINDDISVSPSMLRESKVAKPSINKYSKFKSLLSQNIIDIDSLRALSWGGIPMEHRPLAWKLLLNYLPIEKRNHNRVLMEKRNQYKQLVHKFYTDDLNPNDNKILNQVRLDVPRTIPRGFNKTNLIQSTILHNVLERILYVWSLSNPLISYFQGLNDIPAQFLLIFLSQYINLFGDLSDLTDDILERVEADTFWCFSLLMNNLKNRFIDFHDGIQRMTLKLEKLVKLKEEKLADHLKSEGCDFMLFSIRWMICLLCREFDFSLSARLWDSYIAHGPNFGYFHIYICAALITTKEWVPFLHEKEFSEIIVFLQHLPTEQWNICHIDYLLVRAYKIFLLEIKSLVSDSFKSISSVPIQKVEQQQQQLEDQNIISQLDLNNNSSSNNNNSGINNNYKHPFIRKQNNDKNNIRMDSSINSIILKSQMKDISSIYNIIQQNDQTVNSNNNNSNNSMENNIQLKKLQELHQNMIVSSFANNSQFLNQELLEKVLELIPGQKLDFSTLSPEDADQELIRQVTVYLMLIMGTLLLVSVVLVVIIISTALTITSLEFYNNHSNGVVGNNKLPIKATTTTTTTTTTTEVTNNTNTTSNTTTTTANNNTEDSTFQDTIEND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8D1</accession>
    <name>A0A8J4V8D1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pleckstrin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003786</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000123">
        <property type="protein sequence ID" value="KAF2074909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8D1"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053086">
        <property type="entry name" value="RhoGEF_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45834">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45834:SF11">
        <property type="entry name" value="RHOGEF DOMAIN-CONTAINING PROTEIN GXCJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="3">
        <location>
            <begin position="278"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="497"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="550"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="689"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="126"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="211"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="550"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="570"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="580"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="689"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2074909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="723" mass="80586" checksum="06BD5151AE31B715" modified="2022-05-25" version="1">MHRSSVTSRQAIAIKSKSSPIVSKSSHRVWCIIKIQAVIRRWLAMRLLKKLKESYKSNEFVLKLKDHQQDSTPIIVIDKESDSAEIGSTAISTNVLKQDHQQQQKDQEQINNNDVKSIQIQSSPTQDIDNQLNNDINSSSCSTSSISSSNSSDIKKDQLLIEPLDISSSNSLIIDENETASNSSNETASNSSSETASNSGNEKEISPKVTQNNNNNSNNQQNNNNNNNTPFSSRSSSISSSISMSSSLSSSSLNSSVSDSSSSTNTQVTTSSNHQSLMRKRTLEELLKVEKGYVQDMTTMIDVFLDPFKTEACFPKHHIYTLFCNIEELKRVHQSFLDSLENRLNGNQWSETESTIGELFFPYMGSWQLMYEKYINNYEKAFALTRYFKISPNYAEIQKSIYQKEKDTRCRYLDFNSFLVMPVQRLPRYIVLLQAIEKYTSQDHPDFGQVSLALVQLQDLTLYLNESKRKASQEDKIDEIKLLVQGVDPILSVGQNKFVMEGPLSLLKGVVASTKKDLYIYLFKECILLCTEGCSNNSLNSSKKTFLKSSSSSSSSSSQQSSKNLSASSPTLPRSESNGNLSSPSLSTLISSPNSSSRNNSPNNTYQKTLFKSIAFSDVKAISILKNFKDSFQVVTSKKVYTFVASSTENRDKWVNSMNDCTNSITPPPSPTLTRSNRRSVQLATSQYNSNLSNSSNSNSPSSSASSTPKLTNKKSNRLSVQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8I8</accession>
    <name>A0A8J4V8I8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SMCHD1 ribosomal S5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_000829</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000017">
        <property type="protein sequence ID" value="KAF2077867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10036779at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038892">
        <property type="entry name" value="SMCHD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055109">
        <property type="entry name" value="SMCHD1_S5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22640">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES FLEXIBLE HINGE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22640:SF2">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES FLEXIBLE HINGE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22899">
        <property type="entry name" value="SMCHD1_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMCHD1 ribosomal S5" evidence="3">
        <location>
            <begin position="408"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1058"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1697"/>
            <end position="1731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="374"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="2074" mass="232897" checksum="84ED65F858AE5E3C" modified="2022-05-25" version="1">MERKRKSVGGNGGGSSEASTPNKKALTLPPSIPSSPSSQLVVRNIPDSITISRRGIRHTIDTPSNYKELLIKINHLYQLPKDYIISDLSSNRVDNEASYQSYLNTSNNLLVVYSQGETPLETSEEIVVDITPSKNILLKAGTSEYNFTNAIAEFIDNSIQAVRDNEVNNRIVKVNIAKPNSSTNLTTITVWDNGCGMNKDVLQRWATMGLSQADLLTMPEDQAISASGKTEGSHNFTGLISRYGVGGKKAAFYLGEEIVVSTKVKGSRWINEARISLDILASTGDQDWKIPIVIREPTKEELEWGQHFTFVTIKNVALDSTTYPEQMLFLKRDLAHVYYYYLHDIGKFDVSNLKINTMKTRRATHDIDTANSDSESDDSDSDLDSDDGNQQVGYLLQINETSLHTIKDDMESLYIKYGKRFKTFELNVPLDNNKTSKVLCHLRYFPFKDALETLPVPYRLLHEKEKLDYEYSPLSLRKPGIEIFWNGRLISEGHIDRLGFMSSGTKIDGEKIEDKWVQRVKGAIFLNSDFPVTHNKMHIIKESPIFQSLIKSQNRSQTLDWKKWVLACHKLDQDFQIEGTTDYHPSINRTICKAVSFGGTTYTAGDGVSINNRPVTYGIIRSIYFTGTQESRSSDIQFEIEKVNYKPLPTESIPGAKIKAKMDKKDMSSLITLAKNKEPSKLKLFEGDKVDLPKSEYLSGEKIKWITVALYDKSTPPKEVEKRILEAENIKMGFTIKYKPPQGGQAIEVYKNESSVFYQSSRVSFKDIGCLKKQGTYTFIFSCNYQGVADINHTVIVKPGKVDRIKAEFGCDLTELSQFPIGVDLPPIIVEQIDQEGNMLPTNSIPKTISFTAVKRSEITNTPQDVDDRSNILQLSVPKQLSIKDGRLVVNDLKIVSASLNEEDKMGVLITCTIQTFTCELPLIQIISGQPVSMFISEKMFISDPEKPSFKNHSFLPDFSIQLLDKDGNFCKVSNKKLASTRSRRKPAAPANTPTPNYYIAVSSPFLKDTFLFTPDENGLFSLNPSNVLQTNKKKGGRSSHQNTGLLIEDNQTYMDTDADSSLPTLRKTRSSSKEKDQDIHSIMPILFQVGQGDSQLFEMEQKIKVVPSDRPAFLDVTKMDEFVVDTGDDTFKVLAGTDIQFYLKVRSCGGGGKGPFVLGSVNGQLNTNWSSKVTSVEMSPTDNTVQLPILSADKSAKLIKYSIEYTVEYEGEQICLKKQFSIRTIGGAPTSFFLQRGKGSTEKIRCDSEVSFEVSLFDRKGNTVSPANLTSGKPIQPFFELEIQDGDSPSNGREFSIKNQVIGEFNFDKNVYPCKLSILGLGKMTLKVVDNSGQINSHSIKVILREGSAALLTVNDLPQLTIPCAGGEQFDDLRINICDSQGNRSMDITGVDIHFEWEDLNGRQPPFDIKSKLIIDKGEGYFKGIAVKNTAQEGTYKLILQPGITIERAVILFQVAGNYSMEFVNPDSLLCSLNAPPKPFKIQIISPQKKPVQLNIASIKALSLHQTFPCTAYKDDCYIFDYKVPRKAGPHKITFVLNENGLRLENQQTLVVLPDLPSRLHISGQISSSTSSSIGSNIAVYVTDNFDNRVNVRGSMRVLIEKSIVENSANSSQNIDVPMFLDGKNYQDTQFNDSGEAFFGNLRLLDSIGISGPYKVCFTPNITSVADQPEVKQFNNIQSLSKEFFYKNAKEDIDRNVELQEKRVSLRTEIGKIEKQIADLNKEQDNITSKYKNDMAQAEDISRKLCQMIPGFANVSQNPELVKNLIAESSKDLDTLVNRNRRTAPLFNPVSRELNDVSRSQGEQSGIVGLVNTLIFVEDEREATSYSKLVGKKAEAVIFSNKDDFQSYNKKYREQAQSSFYAVSLDNMIPFSRQPRRQNGYNNQQSQDVTEQGLLNLSPQEGAPLVGFLGYSCNRVQLREKHQHLRKTLVWSLFRDTMVFDTMEHGLAYRTFLARQNRPCSPIYCITEAEYIETSGFVEVGGAKRGGTCCFGQLPVSETEEYKQLYKKKSLLSDYKKVRHQLYVDRKNEVHPQLSGIKDQINELTRLKEQKLNTLNDIFDEIKILETPFSQRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8Q7</accession>
    <name>A0A8J4V8Q7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein arginine methyltransferase NDUFAF7</fullName>
            <ecNumber evidence="7">2.1.1.320</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_000685</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Arginine methyltransferase involved in the assembly or stability of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)'-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48108"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:88221"/>
            <dbReference type="EC" id="2.1.1.320"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NDUFAF7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.320" evidence="7"/>
    <dbReference type="EMBL" id="AJWJ01000013">
        <property type="protein sequence ID" value="KAF2078047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8Q7"/>
    <dbReference type="OrthoDB" id="438553at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035243">
        <property type="term" value="F:protein-arginine omega-N symmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003788">
        <property type="entry name" value="NDUFAF7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038375">
        <property type="entry name" value="NDUFAF7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049">
        <property type="entry name" value="PROTEIN ARGININE METHYLTRANSFERASE NDUFAF7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049:SF7">
        <property type="entry name" value="PROTEIN ARGININE METHYLTRANSFERASE NDUFAF7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02636">
        <property type="entry name" value="Methyltransf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="29"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2078047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="498" mass="55807" checksum="FF19979F366C0AEE" modified="2022-05-25" version="1">MINRIGILNAVSKIKLNRYNELKRFNRSFTSAPNSSSGGNDNTNNNNNNNNNDKNNDSTTTATLDHRATGDVKAVSVSFDKTNMSQFPTTIHTKKKYPITELEKHLTTIAKVRGPFPVDTLIKEVLTNPKYGYYMNKDVFGQGGDFITAPEISQLFGEMIGIWCVSTWEQMGKPEKFQIVELGPGRGTLMSDLLRCTKVFKEFYAALAGVHMVEASPALRKLQKEKLLYFRDDALKNDAVMTGKTPHEISVTWNQRLEQVPQGLPTLFVAQEFFDALPIHIFQFNKDKGWCEVMVDEDITYGGPYHLRFVLSKGPNSLVKAIEHLLPEFGVDGYQVELGLAGLAIAQEMSSRIEKSGGAAVIIDYGYDKIIKNSLQAIRDHKFVDILDKPGSADLSVWVDFQTLRKCVKLMKNSTTAIGPVDQGIFLKEMGIEHRLVQIANKEQSQSKVEELVQGYKKLVDPQEMGSTYKVITISSKSITPVGFSTAKEYEDEDFMTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8W7</accession>
    <name>A0A8J4V8W7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pinin/SDK/MemA protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_000502</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pinin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000009">
        <property type="protein sequence ID" value="KAF2078212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8W7"/>
    <dbReference type="OrthoDB" id="21283at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039853">
        <property type="entry name" value="Pinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006786">
        <property type="entry name" value="Pinin_SDK_MemA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12707:SF0">
        <property type="entry name" value="PININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12707">
        <property type="entry name" value="PINN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04696">
        <property type="entry name" value="Pinin_SDK_memA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Pinin/SDK/MemA protein" evidence="10">
        <location>
            <begin position="103"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="21"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="231"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="134"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="232"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="269"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="303"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="316"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2078212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="327" mass="38506" checksum="FA4FEA933C67A447" modified="2022-05-25" version="1">MSFRNQSNLIHKLQVLEKAKVKTEDRLERLETDQQETTTTTGKRTRNESLYDQKSNKKYNDNAYYDRMFQRKNAAPAVVVVDKTKEEQDKKPATGAPNRPAAANRNRMFVGLLNKTLQDFQKDIVKKTDSDIKREEVDQKIDKIMDKEKEEAEEREKVKKQQMIEKEQKQYDEILKKQEIIENRLLQESWSLHKKTLSSFPYKTKTTPSIYFTLSPLADKHRFKLQKYFPVSEKKTSERNGGHGHTGRDKDRDIYDKEKEQKRRKRYDDNDDDEEDEEDDENNHHGADDMKEEETKKPSTESEKEEGEEREEGEEVSGKDGGDTTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJ78</accession>
    <name>A0A8J4PJ78_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001106">
        <property type="protein sequence ID" value="KAF2068245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJ78"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="399" mass="47031" checksum="AECCE853A2F37CAE" modified="2022-05-25" version="1">MKRILECVECKDDNDLKDKFVEALKDTIESFESSRVTFNTACTIMANMSRILYHFKLIHLNDDNDYRDSLSILWSQARWILSYCFNTATKTKPFKKRLYLRNAKRFGQNYYGNTTKKNKLFKKAFGGDIIVQRKKPFKLIVLDKSNLHPTRIHQRSTNVLQAYHDEEVYDEEHYDEADDEADQDDDNIINDNDRDNEESDGEYDIWEENEQNQVHDGESMPPTNNNNLYDDDDRCEVVMSPPPPINNDLLYDDDDDDDRCDVVISTSTINDMIDDNRDEMVIQGILSLQTAEPFNPPPPKFKINEKVFVRSYEFNPNTWWSSSIVHILPAIIYQDQTYRVKFDQVAPNECEHQLTVEKDIIRLDTFHLDQAIMGEDVIIFYEKKFYLGQYQGEKRRTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PK47</accession>
    <name>A0A8J4PK47_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">3-oxoacyl-[acyl-carrier-protein] reductase</fullName>
            <ecNumber evidence="2">1.1.1.100</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_010507</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17397"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="1.1.1.100"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01001159">
        <property type="protein sequence ID" value="KAF2068165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PK47"/>
    <dbReference type="OrthoDB" id="417891at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032787">
        <property type="term" value="P:monocarboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050259">
        <property type="entry name" value="SDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009093">
        <property type="entry name" value="PRK12429.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879:SF2">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE FABG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00048508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="283" mass="30822" checksum="53D9DC179F450B19" modified="2022-05-25" version="1">MMNRLFNNSNGVLNNIRYYSTGRMNGKVCFVTGGASGIGLGIVEKFVKEGGKVAIADLNKTGAIDLSNKLNQLYPGSTIPVGVDITDEEQVKQAIDSTVNQFGGLDVVVSNAGFQHIETIETLDLSVWRKMMAVHLDGAFLCTKYAMQQFKKDTVRGGCILYMGSVHSKYASEYKIPYVTAKHGLEGLTRGIAREGAKHNIRANLICPGFVLTPLVKNQIPDLAKKFGITEEEVAKKILLKDTVDGEFTTPEDVAEVAVNFSAINNKCLTGQSLLVSHGWIME</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PK70</accession>
    <name>A0A8J4PK70_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001121">
        <property type="protein sequence ID" value="KAF2068225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PK70"/>
    <dbReference type="OrthoDB" id="10680870at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="599"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="599"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="690" mass="80438" checksum="942108F91FA8B50B" modified="2022-05-25" version="1">MKSVVRYCSKANETVSKKYLLQMTKEILNDPLIPGTFEPNWKVSDHSKNCTNDLCLTIGCPKRDFCPVPIQEVRYREDYLKVGVREQHTINYEVHSERTLKFVIGLEKTPNKTMERKSEILDQPVSFYYGLAPYRHHVVLSMFVDSLNMTSSIGELSIQSKFKPLAPTTINTYATQIRTLGDFLACQILYRYGVGNSDEMPLRNDLLAYCTDPSVYYMMFRFFTLEMKRSLTLGINFLNACQRFLCWMHDNLRELNDKKLTLLAPSKEVTATCQHTIACIKRIVSQYSRDSRFQVMNNNSLDSLIKKNKWTTIGNVVVGRKKALKEFHNRLAQFKKRNYLRPKTIIPPKGKKYHENVNQARTIQKTLLFLLETGMNPLRPQNWYLQTQSKKMNVEMLSSDKLNYLCWPFDSKDQDEIKKVYLVFNTYKTRSSGNSLQWCLSQDLSNLIIEFIKFVRPWLISNEDWIKSKNSNGRDPYHFLKTGTLFKNIDGTDSSISIISSSINCCFYKFVGYKIGCSIWRKMIATYKRLSVTIDKNVQVLMSEAQLHSDSIDGSYYNLQNFQKNNERAHQGFGKLMSEFGPNGTDIDLETLFAIYQEEEEEEEEEEEEEEEEDFQHYEDNEDIEDEQDQGWSSDDDEEKEKECKETFTSQKRLRSSSPSSLRKKIKCSLPNEPISTYDDPIIILSSDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKC1</accession>
    <name>A0A8J4PKC1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_009328</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000680">
        <property type="protein sequence ID" value="KAF2069357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKC1"/>
    <dbReference type="OrthoDB" id="20736at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827:SF85">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827">
        <property type="entry name" value="RETICULOCALBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="7"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="43"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2069357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="164" mass="18707" checksum="2DC49E60B70E2C1B" modified="2022-05-25" version="1">MASIDEQVGEELDNFLTKFDKNNDGNVSYDEVFNYWKTQKCKDPKALSDLMFATLDKNGDGIISLSEFTNNTVSVKRLLAKNKAVEDNLVNFLLKFNKNGDDVITRSELEAEFKRSGHENPKKAVNYILYAMDADDNDSLTMNEITESFKLKSAVYKNRQTPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL20</accession>
    <name>A0A8J4PL20_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009616</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000757">
        <property type="protein sequence ID" value="KAF2069069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL20"/>
    <dbReference type="OrthoDB" id="17470at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499:SF136">
        <property type="entry name" value="GH08630P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499">
        <property type="entry name" value="POTASSIUM CHANNEL TETRAMERIZATION DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="63"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="162" mass="19167" checksum="2D58C7D0B3E2CCB7" modified="2022-05-25" version="1">MDNNKNDQQKQTTKLIEDIDLYIENLNKTYNDDLVLMKEEIQLLEEKLFIEYKKQIQDSMTSHPITLNIGGMKYQTTKETLTRIPNSYFHLMLSDDIDHRPKKHKPNTYYIERDGVSFRYVLNYLRDGKLSTASVPTSLKVDVRNELLFYKLIDTKNNLLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL31</accession>
    <name>A0A8J4PL31_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010568</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001203">
        <property type="protein sequence ID" value="KAF2068106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL31"/>
    <dbReference type="OrthoDB" id="659430at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="431" mass="48360" checksum="67A2422229A51472" modified="2022-05-25" version="1" fragment="single">PSFISSRLVNGVFYIYSKSKNRITIPSNTTHLFWLDDQYIDNDGDGINIPPSVHTLVLDRIFNSAILSLPPTITQMIFNGDFGQPLASISFPPTLKYLSFCELYNDIQEQDLPNGLSHLSIGKTWNIKSLPQSVHHLEFNPMHNDFPIPPQVKHLKFQSSGEIFLPPTIKSYISPHSNIIIKEPTDNRLEKSSPVSSFNSDSTSIHFYSDYDFNSFIVPHTLGNNIKSITFGDSFNQVLIKGAIPNSVEKLVFGDRFNHKLNKSILPTSLLTLVLGSDFNQDLNDNLPSSLTELMLNTKSKPSFTSSSQFPPNLKKLALPCYDGVLDIVPTTINHLEFNQNIFQPEYVIFPIELVPSHITSLVLNDSMRIHSYDLIPASITSIKLCESITLDTKIPCTVESVALSSFNQPLDTILQTVDDQFIDVLQFNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL72</accession>
    <name>A0A8J4PL72_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphoenolpyruvate carboxykinase (ATP)</fullName>
            <ecNumber evidence="3">4.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_010077</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>oxaloacetate + ATP = phosphoenolpyruvate + ADP + CO2</text>
            <dbReference type="Rhea" id="RHEA:18617"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.1.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphoenolpyruvate carboxykinase (ATP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.49" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000913">
        <property type="protein sequence ID" value="KAF2068599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL72"/>
    <dbReference type="OrthoDB" id="184182at2759"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004612">
        <property type="term" value="F:phosphoenolpyruvate carboxykinase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00484">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.8.10:FF:000001">
        <property type="entry name" value="Phosphoenolpyruvate carboxykinase (ATP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.449.10:FF:000002">
        <property type="entry name" value="Phosphoenolpyruvate carboxykinase [ATP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.449.10">
        <property type="entry name" value="Phosphoenolpyruvate Carboxykinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.8.10">
        <property type="entry name" value="Phosphoenolpyruvate Carboxykinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00453">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001272">
        <property type="entry name" value="PEP_carboxykinase_ATP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013035">
        <property type="entry name" value="PEP_carboxykinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008210">
        <property type="entry name" value="PEP_carboxykinase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00224">
        <property type="entry name" value="pckA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006820">
        <property type="entry name" value="PRK09344.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006821">
        <property type="entry name" value="PRK09344.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30031:SF0">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE (ATP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30031">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01293">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006294">
        <property type="entry name" value="PEP_crbxkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68923">
        <property type="entry name" value="PEP carboxykinase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53795">
        <property type="entry name" value="PEP carboxykinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2068599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="557" mass="61980" checksum="283172208B79822E" modified="2022-05-25" version="1">MNQSVADSIQQSLKSSTAEFSQNELHVADTDEVNLDEHFTGNAKIYHNPSVAVLYEQALAFEHGSAITSTGALVTRSGVKTGRSPKDKRIVKEPSSEKDIWWGPVNMPLDDLSFMINRERAIDYLNTQEKLYVIDGYAGWDPKYRIKVRVICARAYHALFMHNMLIRIATKEELKSFGEPDYTIYNAGQFPANRYTQGMSSSTSVSLNFAKKEMVILGTQYAGEMKKGILTIMMYLMPKMGVLPLHSSCNMSKTGDTTLFFGLSGTGKTTLSADIHRELIGDDEHVWTDTGCFNIEGGCYAKTIDLSREKEPEIFDAIRFGAVLENVVYNPITRVVDYNDTSITENTRCAYPLEHIPNAKFPAMGNHPKHIIMLTCDAFGILPPVSRLTPDQVMYHFIQGYTAKVAGTEVGVTEPTATFSSCYGEPFIVWHPTKYAEMLASQLHKHSAKAWLLNTGWTGGSHGVGSRIKLAYTRAIIDAIHSGELEKVETTKMEIFGLNVPVSCPNVPADILMPINTWTDKAKYKATSEKLAKLFVDNFKKYQDKASSDVLKAGPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL90</accession>
    <name>A0A8J4PL90_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010044</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000901">
        <property type="protein sequence ID" value="KAF2068629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL90"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="314" mass="36444" checksum="D128AD5D62E33C24" modified="2022-05-25" version="1" fragment="single">MVIYDQWITPSFNQDWKTIEDEYDQKHFFLNVFLDKSGRVTRKKLEEIPEGTTSIKIVIYFDEGLKDHIDRYHDFDKGYVPSSVTRVKIEYNYSWEFEQCHGSDGWLPDSVTMLTVPSLPFPAALVPKNIRYLKVQNQKKAIEIEMVPPITEYLWIEGLSNTIGPNIVHSIPNTVKELVVDYDVLNKIHIKPTFTSSIHINNPHMYHHGDKFSDNEPNNTLGDLNIQTITSSTPVIARLPTITSFVIEEHRSFNNNLSALHPLPPQLKYLSLPFTYDQPIYQQQHIPISLTHLELSIIIDENNHFTNNWLCRKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLE3</accession>
    <name>A0A8J4PLE3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009553</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000739">
        <property type="protein sequence ID" value="KAF2069128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLE3"/>
    <dbReference type="OrthoDB" id="1055097at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="60377" checksum="A85D79D92E9D3048" modified="2022-05-25" version="1">MFFKKHNNILFFSIWRDKYIRRTISNNRLKNKVIPLGVGYLERNHEYFELLDRSSFNILLEIVLDNIELQSLIKSEYSSLIDSLNIDLCKAEHVKMWDQTVIFKLPFYLKRITLTTSLETRFDLKCLPNTLEYLSIALRKINVVGDLAPGTALKTLILHDCPANYLARLLMPTLEHLVLDSFQGELVLDTLPSNLKTLAVNHGRAPIGTPPDSLTDLRMNERPIEALVNITAPNKIYPNVVAKVDTPASFELVSQLKWLTHIIISNATVVGTNRIPSHIRKLVFNQYNLPLPLNLLPRGLVSLEMGMFNQTLVKKAFPAHLKTLILPHFNTPVEKGVFPVALESLSVAACLYPLDLPPQLKEITLTHLCVLPPNPPRTLKTLIVKGGRGGEIDIPKNSIPPFISTLILQGEIKVDSFHLPLPTIRAIHFQRTTSIRLSLPTELIPPSVTDLSIDNHICISPMIPNSVEKLLITEFSNRCNLPASLKRLDITASDFVYEPYVGHYIPINLTQVKENLRSSIKNPSCTIKINGIFYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLM2</accession>
    <name>A0A8J4PLM2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009542</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000736">
        <property type="protein sequence ID" value="KAF2069140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLM2"/>
    <dbReference type="OrthoDB" id="10690159at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035256662" evidence="2">
        <location>
            <begin position="23"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="218"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="295"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="915"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="915" mass="87848" checksum="5CD0C251D4A691DA" modified="2022-05-25" version="1" fragment="single">MRFNNKIFLIFNLILLILVVRSQDPVQVDFSSVFAPGTYTLPWTDSSFRPVGLSPYENVPYFIGNVVLKSQINDEFLSNDLLKDSDLHVLVQHAGLACCRNATSTIKFDFNGNAEGFILLISAIDASDYLSIDSYTTDENGQLVKLNYDQDWITVSNGTFYSTSTSHGPLYEALYPLKPISVVLITYTSLSDSQYVFYSIIDYPNYHPTLTPTQPCVTTADTSTGMTTGEESSSSSDLSTTGEGSTGFTTDLSTTGEGSTGFTITGDASTTGEGSTGFTITGDASTTGEGSTGFTITGQSSTTGKTTGYTTTGKTTKGTTGYPSTTGKTTKGTTGYPSTTTGKTTKGSIGFITGVSSGSLSGIQTGGVTVAGTAGITISGAQSLSGTTGFSVGHVITGASLSGVSTQTIAQSGSVTGVTGGGVISGGQSVGSFTTAQYIAGGTIQGSITAVSSNTLVNTGSGVATGLSSQGVISGGNTIGGVIGQAIGQVTNGQTVSGGSGNTLANTVSGVSSIAGTQGVIAGGQSLGGVIGATVTGVTSANSIGGVSTQSLGNTASVSGVSTVGLISGANSISGGTGNVIGQLTDQNSIGASLSNSLANSIGGVTGHSVTGLNTIGEAISSQVVAGLTTANTIAGATSGSVTGFSSVAGVTQSITGAGSVTGLTGNTVGGVIGVNSIAGVSTASLGNTGTLAQITTQGVISGAGTVAGATGQVVGQVASAGSIAGVSGNSLAGSLTGANSLAGNSQGVIAGGNSIAGVISSNVIAGVTNSQSIAGGIGNSIAGGSSISGVSSILGGAQSIAGVTGNSVTGLTTANTLVGVTTATIGQTGSITGVSSDGVISGVGSIAGVTGQVAGQAIGANTIVGVTGNSLAGSIAGGNSIAGSAGVIAGGQSVGGVLSGQVVGGLTSAQSIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLR9</accession>
    <name>A0A8J4PLR9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ras guanine nucleotide exchange factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_008382</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000511">
        <property type="protein sequence ID" value="KAF2070302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="21144at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF237">
        <property type="entry name" value="RAS GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="6">
        <location>
            <begin position="522"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="5">
        <location>
            <begin position="773"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="209"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="320"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="401"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="692"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1048"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1150"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1246"/>
            <end position="1341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="33"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="160"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="230"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="253"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="264"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="328"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="339"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="401"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="710"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1048"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1060"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1155"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1186"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1210"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1269"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1308"/>
            <end position="1341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2070302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1391" mass="156523" checksum="AE8F1AF91478546B" modified="2022-05-25" version="1">MEEKEHKEPKEKKSLSKIFKPFKREGSKSVMLSSPPIIENSSNSNSSSNNVKFSESSLVRSESNSSISSTVSISSTASTPNMTQGRPRSKSEKKDKHGTLSFNNILAKASRRKSRLFSTPIDPEMIANGNSNGDTINSNGSGSGSGDSATFSNLTSSEDLQQQQQQQQQSLSQSEKKKNRNSIFVRLLRKNKDDGNQSISEEDFKIESNSTHHNQHPHHLTNSHFTSSPNLLANSYASPLNNNNNNNHPHHPTSLTMSVQTAPSNHHINNNSNSNIKSTKNKEVEREEIIKKIQLMADQLNDPSNKTQNTVSINPNHQHLRSERQDLNHSNSSPQLIFNKSNNNSSNNLNNKQLLEENNNPLNNSIDNIKKWRDSEEYDDLSENSTLNDSMVTTEGLLSSPLSISSSSIPSSLDSPPSNNNNNTIRKRPAHLRYTVAIPKFDASMDFNGNPFERLLFALRNSTDPIFNQNRKSTGLSSMDFKSLQLIDNDNQQNLTSLSSTLNGDKQTLPQSVLDGIEEEKQKRQILLICLKKMIQKLVTESDDSALLKTFFSTYECIYSSHDILNEFILIFKNESPQIQLRICTILRNWLKDYYISEICNINFVNTFKDFVVDIKDMENSYLYSKIIEIRNQLIYLLETKINERDLGKYSQKDLENNIQDIEKQYLNKKSIMKIKTFLSTSLKSTGYLIEQHSSPQSISPPPSPKNHRLSGGNSSLSSTSLEQHNGNDELSNFDLNYAYYDNRDRIHNRVPTRPRSSTWSTSDIFLSIMDAPVKEIAKSLTAVDYSIFICIEAKELLNGAWGKPHLKDRAPNITKLISRFNEVSMNVIQSILNEEKLKDRCKVMARFIKIAKHLHELRNYNSLMAIYAGISHSSITRLKWTKKILPKTHQKTLLDLEKLMESDENFKNYRNELKTITTPCIPFLGLILSDITFIQEGNGDFVGDNNETWSLNMNKLKLMYNCIKQIQFHQKTSYLLNADPRLALLLTPNLNVFGEPINIHSPSRPSSPMRQMNSESIVVEKQQIDLSSSATLVKDDDEDFDINVDIEDDDEDEEEQEAQDVKLEKQEEQQQNKKSLDPALRILKFQKKTSEHDLLYRSFTLDTPVRKSSYSNTWSASKLKFEFLQSLSTSSGSVPNLLMERSQSETCLPLSLKPKSTNPNDLINSPRQTTVKSSNENDVAAEQQNENIESSSTTINSTSNSPNLSRIGTSSSTPSSPSNNNSNNDISITITPIEAVNNDPILLLKNNTNTGTFRKNRRKSVSTSNIHSINPNSPTANTTNNSNSGNSKSNNNSQETSPSTLKRIVVNNNNNSGQNSPNSGNTSPVGSTSQHSITHSHSSSFSLFKRKSLSIEPMLINRPSPHTFAPMTDEGLFSLSLKLEPRGVKLSELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM10</accession>
    <name>A0A8J4PM10_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010029</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000891">
        <property type="protein sequence ID" value="KAF2068646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="248320at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117289">
        <property type="entry name" value="Nucleoporin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="412"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="659"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="747"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="892"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="953"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1368"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1482"/>
            <end position="1561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1621"/>
            <end position="1677"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="689"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="412"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="448"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="477"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="754"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="897"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="910"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="954"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="984"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1003"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1060"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1146"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1169"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1196"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1217"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1253"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1369"/>
            <end position="1381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1382"/>
            <end position="1401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1482"/>
            <end position="1496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1513"/>
            <end position="1532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1533"/>
            <end position="1552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1621"/>
            <end position="1637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1663"/>
            <end position="1677"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1677" mass="178703" checksum="C6145A241C8882F2" modified="2022-05-25" version="1" fragment="single">MTEIQCVDVKHIFFNDFKADTLVRIDAKNLRFDDDAHQCHFQRQSNLLVVSNRYGYVFYVTPRGSLIVKNVNQILDSIQTTNNKEIDITISTPTTNTIAQAFNTAQFEYQLAKVSLSNNDQYLSMSYKNVINIYPVSSLINSSIKCEPIQISFSRNVDSYDWGIHQSCLDSILVRCVDSTCYSIQVNQKPQLVGKDISYCCWDTNSDRKWVSSVSNGAIQCLDSSNRVIDTKQYPMNEYDKYIVNYMKRINNTLLVQYDHTKDEIEYIESQFYLYDLSKRDNSKPEYEFYPSLLPADYRTQYYNHFLKEWNTLLFLSNKSASIDCYQWTDNTISSLSLPEGFRADVVNPIGMDIVKCYNKNPTNLTGTKEKSYPPTPIVMVLDANANLLIFNFFNLASKGIPQIQSPSIQTSIMPQSSSTFGITSKTQPAQQQQQQKTIPIVSPIKSSSHHHGHHRNLSSSSQVFEQPSKKSRDSKSAMMPSSAPPAAANNNSVSSILNRRMAQLQNSKLYSSKSNVVDQIKKNMQSIQNYYERVKKNVEQVKQYGIEPFHNSTNILSNFESTRNEIELLEKLFATIDNDSIKSSKQSIALKSRVLKTLALSIDLENQIKDRNSPNYQNLVSNRKLDPETVELKEKITNAYRNLSLEIQKFEQSLQTSKYNNSSSKKKSLASKQQPQQPMSQQLIFESLFNISETENNLRNQVEQLEKKLASIKLSKKKIDKLKSTIDIDQLTQLQLSKLTLTEKMDQHKEADTNSSGQLSLNDLSTNGKPSQLHQDSVSAGVDPTTTNNYQNIISFLKRKSIKTIKPQFPEFSNYFNSETVVDPEFEQEDENTSIVISPNTTTFFPATNQQQSLSSPFSSIPIGNAFETSKYKFNTVNNITPIQSYKQQQQISVRQEQQQQQQQQQQQDYDEDGYEDEDYYDDDDDQDYEDDYYQQEDNDDQDYQENTFENEASQDYEEEPEPEPITPPPKPKIEPQLVLQKPATTIVSTTPATNSSSIFGSTTTPSLPSTTTTPSGLFGKPTPGGLFGSTTTLTPFGGTFGSTTTISGGLSLPSTTTKPKEEEKPKPKSESPPSTSPPVVTAPKVEEKPKSESPISTSSPSPPVVTAPKVEEKPKETTPPPAVVVPKVEEKPKETTPPPTAVIKVEEKPKEDEKPKETTTTPPTVVKSEDKPKEAEKEKGSALDLGGFGLGSSKPITDSNKPSPFGTSLGTLANAAATTPSSTPSTPFGKPSPSPFGSTTTAPSPSPPFGTTPSTPFGSTTTPSSTPFGASTTTPSPTPFGSTTTAPSPSPFSATPSTSTSSPFGSTSTPSSTPFGNTTPFGGAAATPSTGSVFGASSTPAFGGSVFGKPAAAAPAVGSVFGKPPASSATVFGTPSSTNASPFGATAATTPTTSPFKPTNGSVFGASAAPASTGSVFGRAPSSTGGSVFGGSSTAPAATFGSTSAFGTNQGAFGGAATPTPGAFGAAATTPSTGAFGAATTPSTGPFGSTPSTGAFGAATTPSTGAFGAATPSTGPFGSTPSTGAFGSTNPSPFGSSASTSSPFGGAAPSTSAFGGAPTTSSAFGQSSAFGGGNTGAFGGATPSTSAFGGAPTTSSAFGQTSAFGGNTGAFGGATPSTSAFGGAPTTSAFGQTSAFGGSNTSVFGGGGQSNPSGSAFGGAPTTSAFGQTSAFGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMZ2</accession>
    <name>A0A8J4PMZ2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008470</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000525">
        <property type="protein sequence ID" value="KAF2070209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMZ2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="72" mass="7660" checksum="229AEE0200F92985" modified="2022-05-25" version="1">MIIKCLSSLAPGKIGGTHSSISMGSGNDAGSGLNQLAMPGSLFFKYGYIDKPDGTRQMVLIYTVTGEIVHYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN03</accession>
    <name>A0A8J4PN03_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_008458</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="AJWJ01000523">
        <property type="protein sequence ID" value="KAF2070224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN03"/>
    <dbReference type="OrthoDB" id="18472at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000024">
        <property type="entry name" value="Probable serine/threonine-protein kinase cot-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF417">
        <property type="entry name" value="CELL CYCLE PROTEIN KINASE DBF2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="479"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="13">
        <location>
            <begin position="782"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="121"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="252"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="868"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="941"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="32"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="121"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="253"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="301"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="329"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="359"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="373"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="404"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="947"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="966"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="996"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="508"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2070224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1009" mass="111061" checksum="4B352C3250F8713B" modified="2022-05-25" version="1">MSISLRESTDSLPPGNSNSNEERASPLLKSSFGYTSPTFMSSRSINNSFNTDDPNDPNSPKFLTSSHSNIKLPNLSEQQQPYGQSHSETNSPTFSAVNSTTSPKRLLGFFSKSSSFQSLLNNSNGSNSSGGNSNNNSQKNIINGRNISSSSSSNNSVGNNNSNNNNNNYNSESPPPPYSPKRILRNEKAKSSPPLSPIPFSSVNNSSNNDNDDSTNSSYGSTGIDSSLFKKPSLFNTSSHLIPNIDSLRLDDSLSSSPPTTTTSNNNNNNNNSESTNNVSGTSSNNSQEDSPKISPRQYRPTLTRTRSNSKNVLYSPNISPSSSCKLFPSPTSPISTNNITSPSSSSPMNSSPMNSSPQAIRNNSNSQQDDMSGGSNSSGSYNRPSSIIPQSPTQNNNSNNSNLILTPQQQTSPPMSIPTASSIVTPINNLETITEQQLLDHSNHNIDILHINNNYIVPSPSPSSKTASHYPPVSIVDFKLIEKIGEGGFGQVFLAKKNDTGEIVALKRMSKDLIWSKNKVSHIKNERDILAQGRNHQWIVSLAYSFQDEQYLYLAMEYVPGGDLRSLLSALVSLDEKSACFYMAEMVEAVDSCHQLGYCHRDLKPENFLINKYGHIKLADFGLSKNVITRYSVKPKGSQPPIEHTPMKFNNNGNALNSSVVDFGSFKDINAQAQLAYSVVGSPFYMAPEVLEAVTGYGDEVDWWSLGCMFYEFIFGVPPFDGDSPEEVMETVLKWKTMLVRPEGVSDLLWDLISSLVCDGSTRLGTGEKGVENIKAHPFFKDVPWGRLHQLDPPFVPVLNDEYDTTYFENTEKVVRSRSPVIYTNTNSGNIVTSKTKPRNILGFTYPRAGDEPLIWNNMNINGNTSNINSSNSNNQSTVNSASSSTNSSLNNLFKNFELTNSSEIKLLRNSNNLPLDLKDSNSNIYNSSLDERRSIIKDKLDNDNNDNNNDDNNNNNNNNYNNNSPFSTPYQSKQQQQDEPVSPYRPFIPFNVNQDDDEDDEDDVPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN06</accession>
    <name>A0A8J4PN06_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008706</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000563">
        <property type="protein sequence ID" value="KAF2069980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN06"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="123" mass="13383" checksum="5D65D8B4771BCF5E" modified="2022-05-25" version="1" fragment="single">GRFIKRGIVDGRVRQISNTPLNTEFKSTSSKSQTHIGISVPHYSSMVQLDPDFSVLVDHKAADIDSPNSVCAAKGKSKLTGAQIAGIVIGCVAFATIAIVSVVYYLSQKRKRSFFIKKLNNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN09</accession>
    <name>A0A8J4PN09_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alpha N-terminal protein methyltransferase 1</fullName>
            <ecNumber evidence="5">2.1.1.244</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">X-Pro-Lys N-terminal protein methyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_009471</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-terminal L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N-terminal N,N,N-trimethyl-L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54712"/>
            <dbReference type="Rhea" id="RHEA-COMP:13785"/>
            <dbReference type="Rhea" id="RHEA-COMP:13971"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138057"/>
            <dbReference type="ChEBI" id="CHEBI:138315"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N-terminal L-prolyl-L-prolyl-L-lysyl-[protein] + 2 S-adenosyl-L-methionine = N-terminal N,N-dimethyl-L-prolyl-L-prolyl-L-lysyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54736"/>
            <dbReference type="Rhea" id="RHEA-COMP:13787"/>
            <dbReference type="Rhea" id="RHEA-COMP:13974"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138059"/>
            <dbReference type="ChEBI" id="CHEBI:138318"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N-terminal L-seryl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N-terminal N,N,N-trimethyl-L-seryl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54724"/>
            <dbReference type="Rhea" id="RHEA-COMP:13789"/>
            <dbReference type="Rhea" id="RHEA-COMP:13973"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138061"/>
            <dbReference type="ChEBI" id="CHEBI:138317"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. NTM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.244" evidence="5"/>
    <dbReference type="EMBL" id="AJWJ01000717">
        <property type="protein sequence ID" value="KAF2069214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN09"/>
    <dbReference type="OrthoDB" id="1298661at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071885">
        <property type="term" value="F:N-terminal protein N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000025">
        <property type="entry name" value="N-terminal Xaa-Pro-Lys N-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008576">
        <property type="entry name" value="MeTrfase_NTM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12753">
        <property type="entry name" value="AD-003 - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12753:SF0">
        <property type="entry name" value="ALPHA N-TERMINAL PROTEIN METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05891">
        <property type="entry name" value="Methyltransf_PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016958">
        <property type="entry name" value="DUF858_MeTrfase_lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="156"/>
            <end position="157"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR016958-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2069214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="274" mass="31482" checksum="2600D780A747C2E1" modified="2022-05-25" version="1">MEKIEYPKKLQQVVGVDTDGQVYKNIDELWQKEVDVNATENKDNKWYKSADDYWKTVTPTVDGMLGGLSHVSSADTIASKFFIKDFIQGTPTRPAISLDRALDCGAGIGRVTKDFLLPLGFKNVDLVEQNELFLNEAKSKFFKDDKRVENYYAVGLQDWKAEHKYDCIWIQWVIGHLHDTDFIEFLKRCVDSLAPNGVICIKDNVAKGSFIMDKEDNSVTRNEAHLKFLFEKSGCKLIKALVQPNFPKELFPVIMFALEKKEDQKVEQQEEKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PND6</accession>
    <name>A0A8J4PND6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PPPDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008519</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000532">
        <property type="protein sequence ID" value="KAF2070162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PND6"/>
    <dbReference type="OrthoDB" id="10533093at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="13843" checksum="3D6505B3E1F13F5E" modified="2022-05-25" version="1">MRWCQLDTKVRDAALTLAARMSLMTSRAAHHHFIIVTLESGDCVYFDKGSYRNIFIVHNTNGLNKFNKWVPSNLMRAQTATKGKTLKDLYNYASLDKFRPYHPLDKNCQHFADALYDWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPP8</accession>
    <name>A0A8J4PPP8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RCR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">CYY_007838</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-threonine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-3-O-ubiquitinyl-L-threonine.</text>
            <dbReference type="EC" id="2.3.2.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Axon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RING-Cys relay (RCR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.33" evidence="5"/>
    <dbReference type="EMBL" id="AJWJ01000440">
        <property type="protein sequence ID" value="KAF2070852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPP8"/>
    <dbReference type="OrthoDB" id="6050183at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16463">
        <property type="entry name" value="RING-H2_PHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000078">
        <property type="entry name" value="E3 ubiquitin-protein ligase MYCBP2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45943">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MYCBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45943:SF2">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="278"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="282"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="527"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="46"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="113"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="212"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="245"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="113"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF2070852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="757" mass="86171" checksum="B7E838AA77C1965B" modified="2022-05-25" version="1">MELKSQLMMNNNNNNNNNKKNTSFGQFLNEAFRVLTSSSIIKVAEQEREEQDDDDDDDDSVHNNGTNNSNQSSTYSSDYSSGEEEMKTIDPNVMGDAFNSLIKNNSNILSIISQSPSSNPNSPNLFSSSSSSTTTATKSKPLDIKFNLNNNNNNNNNNNSIHSNSNSFNSFNSFGGKYPDEGFTPRSFYESPTDPFIDFEFKRLKSNVNLNVNNNNNNNNNKSTTTTTTTTTTSSTSNLKSNVNINNKEIELDDEEERNQKLIQQLMSEQENSDQDKIEMECTDCLKQYPIEEIVFLDCSDIYCKKCLHNFIMAKSNEKKVSKIKCKSCKKPISNYDIKQVLTEQEFSIYQGASLDQVINKNKENFVKCPSCSIFMEKIIPPQTMSFGSPWSTDGNGVRIECDANGKPLSRESIEHKKKYRFRCLECSTIFCTECLVHPYHLGFTCADYQIFNSTKHCRYCKTPLLYSGNGNGCANEECKRLKAKSCKKKLECGHPCMGIRNELECLPCIHPDCKQSNTNQKMNDYCNICWTDDLESAPSIQLDCGHIFHYHCCKNIISKRWSSSRISFGFLKCSLCSQNMSHPSLEKYIKPINDLYDIVQSKGLQRLSHFGPEKGVDLNDTKSKWFNNKESYVMERFSYFMCFKCKNPYFGGEKACGENTVDFKVEDLICGGCSSNGNETCKIHGKEYIEYKCKYCCNISIFFCWGKTHFCDDCHKKVNEVARTPKSALPRCKCKVKHEEGEEYCFGCSLCRILSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPV1</accession>
    <name>A0A8J4PPV1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006829</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000332">
        <property type="protein sequence ID" value="KAF2071862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPV1"/>
    <dbReference type="OrthoDB" id="21650at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.950.10">
        <property type="entry name" value="Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003128">
        <property type="entry name" value="Villin_headpiece"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036886">
        <property type="entry name" value="Villin_headpiece_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02209">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00153">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47050">
        <property type="entry name" value="VHP, Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51089">
        <property type="entry name" value="HP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HP" evidence="2">
        <location>
            <begin position="926"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="670"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="851"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="301"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="344"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="381"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="464"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="670"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="784"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="794"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="887"/>
            <end position="918"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="990" mass="109824" checksum="81FFDB85FE8AC4DF" modified="2022-05-25" version="1">MTKGKKASHGKNQKTGSKSFTIEEPEAYLTIQKEKEEKFKVTQEKDAARKLEKERLEKHRVEQERLEKERLENQEKERLENQEKERLEQERVEKEKLEKELAEKRELEKKEKEATDAAAAAAATTTSAPVATTPAPAVVANNTNNTNTTTTTTSTIKKESSSVALGSPSIAAKKKVSNKRKHSQANITPFEIKEPTDFSITFDNEDVIKSRSKIIKTSKPKKKKNNSKQNSPALATEESELATPPVSISETTKPVETKPAVAVVAKPAVVVAAAETTTPTSTSATNSPSTNPVNKKKKTSKSSSPKAAAPKGFTIVEPTFGDQPKQTAPVVVEKETTKNKKASPKAPSPVVEESTPVSVPTPTTTTTTTTTAAAAPAGKKDKNKKKKAAGKKQDNLDELLQQFKESDIKSSVNNNNNNNKSQPASRQSSKANFEKEAEKAKADSDKKKSAADLVQKLDQVKISQDQEKAKQEQEAEKKRLADEKAEKDRLAEIEKKRLADEQAEKKRVADEKAKQEQEAEKKRLADEQVEKKRLADEQAEKKRVADEQAEVEKKRVADEKAKQEEIEKKRLADEQAEAEKKRVADQEAEKVRLAEVEKQRLADEQAEAEKKRVADEEAEKKRVADQEAEKKRVADEEAEKLRLAEIEKKRIADEEAEKFRLAEIEKQRLADEEAEKAKLAAEEKAKQDEAEKVRLAEVEKQRLADEQAEAEKKRLADEEAEKAKLAAEEKAKQEEAEKVREETQVPSEPTEEDHQKLTHLTLSRPSRKGVKKVSESTTAEDETQSTTETTQETKPVEEEPVKPVEEEPVKPVEEEPAKPVEEASSKPAPVFGGINMLGDAKINLGSVALKKTPSTTKIDTPKKEEPSSGPVDFRSVLKSKSSERLLNSPATANTTPVNTTPVKTQPTTPVVATPSTPAAAAAPSASEITTFYSYEQLKNWKTEKLDVNPATLELYLNDEEFAAVMKMTKAEFTKMPGWKQKNKKVETKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQN8</accession>
    <name>A0A8J4PQN8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="15">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">CYY_006373</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="15"/>
    <dbReference type="EMBL" id="AJWJ01000291">
        <property type="protein sequence ID" value="KAF2072318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="270392at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02584">
        <property type="entry name" value="RNAP_II_Rpb1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02733">
        <property type="entry name" value="RNAP_II_RPB1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000019">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.30:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.390:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.274.100:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.140:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.180:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.120:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.120:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000684">
        <property type="entry name" value="RNA_pol_II_repeat_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007075">
        <property type="entry name" value="RNA_pol_Rpb1_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007073">
        <property type="entry name" value="RNA_pol_Rpb1_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038593">
        <property type="entry name" value="RNA_pol_Rpb1_7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006336">
        <property type="entry name" value="PRK08566.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF37">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04992">
        <property type="entry name" value="RNA_pol_Rpb1_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04990">
        <property type="entry name" value="RNA_pol_Rpb1_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05001">
        <property type="entry name" value="RNA_pol_Rpb1_R"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00115">
        <property type="entry name" value="RNA_POL_II_REPEAT"/>
        <property type="match status" value="16"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 15" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="17">
        <location>
            <begin position="236"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1486"/>
            <end position="1508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1567"/>
            <end position="1753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1496"/>
            <end position="1508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF2072318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1753" mass="195163" checksum="440611DE1CEC7729" modified="2022-05-25" version="1">MAALTPSTAELRKVKRVQFGILSPDEIKNMSVALVEHPETYENGKPKVGGLLDPAMGTIDKHQKCQTCAGTMAECPGHFGHMELAKPVFHIGFIDTVLKILRCVCFHCSKLLTDTGDPAFRQALKIRNSKKRLAAVTDICSGKKVCVIGGGEDEEHDLSKTDEELDKKPKHGGCGNVLPKITKEDLKIIVEFKDVADESIEKKSVLSAERVHNILKRIKDEDSIAMGINPEWARPDWMIATVLPVPPPPVRPSIMMDTSTRGEDDLTHKLADIVKANKELKRQEKNGAPAHIITEATQFLQFHVATYVDNEIPGLPQSQQRSGRPLKSIRQRLKGKEGRIRGNLMGKRVDFSARTVITADPNLSIDQVGVPRSIALNLTYPETVTPFNIDKMRELIRNGPTEHPGAKYIIREDGTRFDLRFVKKVSDTHLECGYKVERHINDGDVVIFNRQPSLHKMSMMGHRIKVMPYSTFRLNLSVTSPYNADFDGDEMNLHVPQTVETRAEVIELMMVPRQIVSPQSNRPVMGIVQDTLLGSSKFTKRDTFIERDLVMNILMWLPSWDGKIPTPAILKPKKLWTGKQLFSLIIPDINLVRFTSTHNDKEQNECSEGDTRVIIERGELLSGILCKKSLGSANGSIIHVVMNEHGHEECRKFIDYTQTVVNHWLLNYGFTVGIGDTIADSQTMEKVQQTISSAKNQVKELIINAQNKQFECQPGKSVMDSFEQQVNQVLNKARDTAGSSAQNSLDESNNVKAMVIAGSKGSFINISQMMACVGQQNVEGKRVPFGFQNRTLPHFTKDDYGPESRGFVENSYLRGLTPQEFFFHAMGGREGLIDTAVKTSETGYIQRRLVKAMEDVSIKYDATVRNSLGDVIQFAYGEDGIDGMFVENQNFDSLRKNDAELAKTYCHRIDDPDYGKGWIDTQVIEEFRSDPMVRDTLEKEFERIKFDRNLLRNELITSGESGWPLPVNLRRLITNAQKLFNIDHRRVSDLNPSTIVNMVEGAISRLKIIAMSDESADDVTYTKAWAETHSNATLLFSILVRSTLASKRVLSEYRLTEKAFQWVVGEVESKFLQAIAHPGEMVGAIAAQSIGEPATQMTLNTFHYAGVSSKNVTLGVPRLKEIINIAKQVKTPSLTIYLKPHCARDLDKAKTVQAQLEYTTLAKVTAATEIYYDPDPEHTIIEEDQEFVQSYFELPDEEIDVSCMSPWLLRIELDRQMVTDKRLTMSDITQCVNRDFGVSLNCIFSDDNAEKLILRIRMVESQETKGSDNDDDDQFLRRIESNMLSEMVLRGVPNIKKVFMRSDDKIPKINENGSYGTREEWILDTDGVNLIEVMSHPDVDHTRTVSNDIVEIIQVLGIEAVRNALLKELRAVISFDGSYVNYRHLAVLADVMTYRGHLMAITRHGINRVETGPLMRCSFEETVEILMDAAMFAETDDVKGVTENIILGQLPPLGTGSFEVYLNTDMIKHAHSIALPDQNNMMMANNFGGNGGDINPGSQTPSYDGSATPFHSFSSYDAPLSPYNETYRGDFSPSSSFNPSSPSYSSGGGKNYGSSFSYFPQSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPYYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSSNYYNKNYNQYNNTNNTNKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQS5</accession>
    <name>A0A8J4PQS5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007549</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000410">
        <property type="protein sequence ID" value="KAF2071120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQS5"/>
    <dbReference type="OrthoDB" id="7600006at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="533" mass="60532" checksum="BA2787B47043D4C7" modified="2022-05-25" version="1">MKNSNNNNNNNNHNNLFFYIWKSKYIKVKILNEIMRDRNINCTLGYIQENHKYLNILDIYKYNIGIQVKVQIENIEEYKEFPLKNLINCLDFECNQYTSICSKEQQLDILENHLPKYIRSLKGVNELLIHQGSLYDTSIETLNVSSIKKKIQVNSLPKNLNTLKVGFYNGTFGKETLPSSLKKIEWCSGINLDNLNKSFHLDKVESKLLMTSVFFPNISKVNNILVTSLNIQSYSIAKYPLKANIFKPSLRELGINILKASPDSTLCCGVFPSGLVKLTLNYSQCQLKPEYFPCTLTYLELKSYNTLLLPFILPKSLKHLILPRYDQPLLVNSIPPFLEQLRLDLFSQTLDAGVLPNSLTALSLLSKPTLKKDSIPCTVKTLDLNMEVMESVSEDMESFLPCSIKTLNLNVKVKATNNLLCLSKLPKSVKSLSIKSIATLDTIGLQLPSSIASLTLHYPSTKILFSPNSLFFPVNLKKLTVSSFIADYLALNSTLMPSTIEYLNINGESKFKPFPIPKHLKIFILNNTFQSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQT6</accession>
    <name>A0A8J4PQT6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Deoxynucleoside kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006559</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000309">
        <property type="protein sequence ID" value="KAF2072114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQT6"/>
    <dbReference type="OrthoDB" id="17400at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01673">
        <property type="entry name" value="dNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050566">
        <property type="entry name" value="Deoxyribonucleoside_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031314">
        <property type="entry name" value="DNK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10513">
        <property type="entry name" value="DEOXYNUCLEOSIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10513:SF15">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01712">
        <property type="entry name" value="dNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Deoxynucleoside kinase" evidence="1">
        <location>
            <begin position="101"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="358" mass="40847" checksum="896BAEC0D92F1077" modified="2022-05-25" version="1">MTILNSIQNFGSFNNTNKQIRSEAGKVQSNSYSSSTQGSNQNADALLYVWCKGCSICNKRFPHCLNCAFTTTPINHTTVNRSNDSMVSQITTPTKKEPKLIILEGNISAGKTSLSAKLGDILGYSVFLEPITTNPYLTDFYREPSKYALVMQLWLLDQRYNTFLNAVKYALEYNSGVILDRSVYSDWVFAENCRLEGLISEKGFIEYKNQREKYLSNIPIPEVTLYLDVKPEECLKRIQTLRKRDCEQNIPLSYLKGLDSCYSDFLKEMNEKGSNVLSVDWTHFGDPNTIANEIKPFLSLPSSLSNCINKNTLSTINDLIEKQKILNKHSHFDQNKTEKEFLIKEMNDTQNNINLLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQV0</accession>
    <name>A0A8J4PQV0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nucleolar complex protein 2 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_007520</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000406">
        <property type="protein sequence ID" value="KAF2071165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQV0"/>
    <dbReference type="OrthoDB" id="10266662at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030690">
        <property type="term" value="C:Noc1p-Noc2p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030691">
        <property type="term" value="C:Noc2p-Noc3p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005343">
        <property type="entry name" value="Noc2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12687">
        <property type="entry name" value="NUCLEOLAR COMPLEX 2 AND RAD4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12687:SF4">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03715">
        <property type="entry name" value="Noc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="19"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="156"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="684"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="66"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="161"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="691"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="754"/>
            <end position="765"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2071165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="788" mass="90874" checksum="21F0B0839DDC227D" modified="2022-05-25" version="1">MKVKKSTKKLDVQALKKQRRFQNKVKKGKKVSDKSLNNFREESSEEEQEFEIVQKQPIKNTKIQQDEVEEDDSDLGEIEGDDDDADDSAEESDLEIEQNESDVANLKKKDKKEVVAESEKTKSLKSSVKQHKEDLAALKEKDPKLFEYLSKQDASVLNFGQDEEDDEEDDESSDDEEDLDDDEETSKPQEKKQTMTSALLESWVLEANKNTNLQNVKKLILAFRSAARTGSDLKDTKNVAFRILNSDIFNRTIIICLQYMPTYFDKLLEYNSQTQDEQKPELPSTTSAKWKQVLPCINSFIKSIIHLLTQVSEAKMLLALLKGLEKVICYVACFNKYSKLFLKQLLQNWSSSQESVRVMAFLCIRKLAIYCPFPFIDDCLKGIYLNFARNSKFITATSLPIVNFMCNCVVEIYGLDFASSYRSAFMFIRQLAIHLRNTLNTNTKEAFQNIYNWQFINSIRAWVEVLAAYPKQEHLQLLLYPVTQIIIGIINLIPSSKFLPLRFHCIRFLNRLASSNGLFINATPYLLDILNSTEMKEQYASKKSKFINFYTALSASHPQLKSKEFQDGVLSQFVELLVENLECYSCHIGFPELCAPVVVQLKKSLKMKYRPKVVSDLQSILEAIEKNVKLVRTARDKVSFSPKESKQVAAFTEQLKEQHKITPLKQLLLTLRKRSKQEQQVLLESQKVYNYEDDEEDQEEGDDDEDDEMDGMEQVGDEEDEDDEEGDLYESGDEDEDEDEEEEEQVKPKSNNKNNKKQPQNKKPKFQTNATDNITEDLVEDLVLSDNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRI9</accession>
    <name>A0A8J4PRI9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">TRAF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_006681</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probable adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000319">
        <property type="protein sequence ID" value="KAF2071992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRI9"/>
    <dbReference type="OrthoDB" id="5985214at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001293">
        <property type="entry name" value="Znf_TRAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131">
        <property type="entry name" value="TNF RECEPTOR ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131:SF94">
        <property type="entry name" value="TNF RECEPTOR-ASSOCIATED FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02176">
        <property type="entry name" value="zf-TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50145">
        <property type="entry name" value="ZF_TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TRAF-type" evidence="10">
        <location>
            <begin position="217"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TRAF-type" evidence="8">
        <location>
            <begin position="217"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2071992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="465" mass="54257" checksum="38F8941CF2160809" modified="2022-05-25" version="1">MEEDKDKDKDKDKDKDKDKDKDKDKDKDKDKDKDKDKDKDQQENILPDKETYLQDNSSPFEDPTFSEERIEILLDDVFVNKNDIYNCSICLEMKPVLYQCYNGHMNCLQCWEKHLSTNKSCPICRVKITRQTLGKNLAIESFVSKEMMRCPYSETCDSMNYGTFKNKHNNECEYRSIKCECLVIMKFNELENHQKQCPKSKLSCSHCQTVFTRDSNHLEICDEMNISCHQCQTKIKRKELIDHVRDACPKTKITCPLKYLGCRGDFYRQDKRTHFTSDQHINVCMGIVSNQPVFMTTLERGTFETKFQFLGVDSSLSYEIEDGELVTNFPNLMNEPFAKDYSFTINLYDKNNNLLESGDIYCLSKYNGKHFSKDFDLISLYDKNNKLLSQTGDEITYVVTELSIESSYETTVGLAKEFKNIIIIFVSEIGYDPYQARRLIHDLVSNMPYSTSSIIFNSVSRELIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRR6</accession>
    <name>A0A8J4PRR6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_006190</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000274">
        <property type="protein sequence ID" value="KAF2072503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRR6"/>
    <dbReference type="OrthoDB" id="5951731at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053331">
        <property type="entry name" value="EGF-like_comC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013111">
        <property type="entry name" value="EGF_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24032:SF16">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24032">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07974">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035152221" description="EGF-like domain-containing protein" evidence="4">
        <location>
            <begin position="19"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1046"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="763"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="789"/>
            <end position="798"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2072503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1086" mass="118462" checksum="22B47AB1A23F8FC8" modified="2022-05-25" version="1">MNLLLILFSLVLLNITNGQSITGTSPSFIRGGNTQLPYFDILGNFQAGTTVTIVANDKTFAVAVADPDAIYSVQLPIFDPPVYSVFLRLAGESQGYILACAYLDNNGFTQDNDMGYITGKGFKYLPGATAYIKDNVVASYHPITVHNDTTLSFKVVPKMGSTLSLYDSWGIVVIVTLAQTVNYNPIIMDIKYYESSFAIYSSVILVSITFNGYQCSITGEVIDEDVYNIACIPAVEILGLEEEFTVVAKDVSGRTTTIVRSFPSKISNTSTDVNGNSILHGSFAPLPTGFKIVGIYSTPLTSISNTSDTIAFNTPDDISCGYVYILANGERITNQVLYCPTPQIVTYNVSNNVFYAVGKYMTGNNFDTFNLQIESSDHPSTCTFKSMVDYTYEIICALNPNFRSAVFVGAFQISGQNIRLNLNNNPTINSISPIIYQKPGIVTIFGRFLKPYYQSGSTEVKIAGSSCSNIVINSDSEIQCLYQSDVPMTNLFEPLEVFYKVEYYFSYSSKLLTYSCPTASSVACTGNGQCELNSICVCTLPWYGDNCSSIDATISSITSTKYGVASQVTITGTNFLPPNIFVTIGGSECESPVAVNTTMISCFFRSNVAADPDTKLQVVVNIGIGNSFKATIFSYLTCVRGRNNNKVCSGHGSCSSDLVCNCEKGWEQSDCSIINPSLTSITSTKYKAPGQVTILGNNFSNYNLMVKIGGSVCTNAIASSDAKSITCLYQSDVATNGENDLLSVYVSIDIYTVAKDLFLYTKPGLTCPNNNNQECSGHGTCNQQQLCICEKGWTNSDCSIKDIGVVIDDPIVNENDTSTTIITPSGTKFDIGLIMVNELNTNNDIIHSFNLNKTNWTQNNDKSLYTATLTNNSTLKVQLTINSLDQYLYYDFAGDTLPILPKSIKYQIELQNWSFGSTQNTLEFIFKSHISESNNECLDSTTAKTSTTSNTGDSNLRSIQMTLNGETLIGTFSDRIVLDSRPTYNKVNQISNERIVLYQLDKSNVYTSIQTSYFKNSVVVDPNFGVLVSSAPDECSNNNGTASWKIAVAVVCSVVGASIIGAAVFMFLKKNSRGKIILLKLKEIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRS6</accession>
    <name>A0A8J4PRS6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_006161</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent RNA helicase required for 60S ribosomal subunit synthesis. Involved in efficient pre-rRNA processing, predominantly at site A3, which is necessary for the normal formation of 25S and 5.8S rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000272">
        <property type="protein sequence ID" value="KAF2072518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRS6"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="178"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="365"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="30"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="532"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="43"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="541"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2072518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="582" mass="65631" checksum="1CC7347DE4FAE396" modified="2022-05-25" version="1">MFRLFKTTTTTPLFNLRGLFNTTQTRLIGSSSSSNRGISFGAQNNNNSNYRSNQFSRSNNNNNNYNNNNNEQDDFSFIESSGGRGKILGDNNYRIDKRVTDWERETLAEIKKQIYTPSEPVDTKVINQFLADNNIAVESRQQIPMPMTTFEGTPFNEKIVRMLKSTFDTPTPIQQLGWSISLTGEDFIGISKTGSGKTLSFVLPAIHHIHNQARSHTYLGPSVLIIAPTRELSTQIAAESKPYLQALNMRYASLYGGESKSLQTAQLRKQPQFIVGTPGRILDHLNDGTLSLKNISFLVLDEADRMLEMGFESQVRSLFENIRPDRQVLYWSATWPSKVMNLANEFIINPIKIQVGNNELTANDAIKQNFKFVETEAHKATALLDTMEEIYTKNPEAKTLIFTMTKQGADKLKEYLTKKGNARIESLHGDKAQNHRSRIIDDFKKNHIDILVATDVASRGLDIKDITDVINFSMPPQIENYVHRIGRTARAGATGHSHTIISKSSVNDMELLNELIDLLERSNQEVPREMVELAPQKNNKRYNNNNNNYNRNRNNNNNRRGGGGGGGYRSQRSGPISFGGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS03</accession>
    <name>A0A8J4PS03_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_006745</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000325">
        <property type="protein sequence ID" value="KAF2071930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS03"/>
    <dbReference type="OrthoDB" id="10264062at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000005">
        <property type="entry name" value="TBC1 domain family member 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021935">
        <property type="entry name" value="SGSM1/2_RBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF502">
        <property type="entry name" value="SMALL G PROTEIN SIGNALING MODULATOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12068">
        <property type="entry name" value="PH_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="6">
        <location>
            <begin position="519"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="314"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="402"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="314"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="337"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="402"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="412"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="422"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2071930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="814" mass="91980" checksum="E3243954653786E1" modified="2022-05-25" version="1">MFMNIPHRILFSKENVFIYPPEVEKGIKGTLSICSLDKGSLYLLWMPANEYSGGNSSNDGNDNNNDTTIQEELEMNPTSLVVRVHIKELKSIKKYTPTIGTPYIIITSKSGTAFYPFFFEQGGVREFLKCLSSIIHLKKSNIDPNFFIVVDLTDPIQRSLSHMNLSESIDKIHDPLADLEMEGDQNKYSLLSSTNHLKKAQEISVSIANVTNNTSNFTKSGLSFSTSILAPPSNVSLLSTSIKESLYESSTMNNSNSYHSGLNTLNSNNDLTKPSLTLNNNNTTTNSLSLSSIISKSPTSTSSSPISINRLAHSGNISPISSPSSKILSESTDSDDGSNSFSTSPTNGSGTPNHRHKLKREFSSSILENFAKVTQLAKSAQKKIFEEPAKNIDNHFRNLMNKTATNSLSPPHSNSHSNNSSGHHHSHGHHNSHHHSHNSNGIPSYIDQLNESSSSIMSSSMDYFTPFNISSSNFSIEIGINRRECNPLSASEWYSYFDEEGRICLANQQVLLKKIFYGGIEDSLRSQVWPFLLHVYSFDSTYASRQVKAHEKLLEYQTLKRQWESISCEQESRFSKYVSRKMLIKKDVIRTDRLHPMFIDGEDELDANPNLKLMYEILLTYSFFNFDIGYVQGMSDLLSPILSVMKNDSDSFWCFKGLMDRLESNFHKDQNGMHTQLSTLSKLIKFMDNDLFNHLEANNGSNMYFFFQSVLICFKREFCFSDVKCLWEVLWSNYLTKQLPIFMCLSIILKEREKILSENMAFDLILKLVNEKANKMDLDELLIDAESLVKYFIIKQPSNDPSLLALKQSISIGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSK0</accession>
    <name>A0A8J4PSK0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phosphatidylethanolamine N-methyltransferase</fullName>
            <shortName evidence="13">PEAMT</shortName>
            <shortName evidence="13">PEMT</shortName>
            <ecNumber evidence="13">2.1.1.17</ecNumber>
            <ecNumber evidence="13">2.1.1.71</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Phospholipid methyltransferase</fullName>
            <shortName evidence="13">PLMT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_005948</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the three sequential steps of the methylation pathway for the biosynthesis of phosphatidylcholine, a critical and essential component for membrane structure. Uses S-adenosylmethionine (S-adenosyl-L-methionine, SAM or AdoMet) as the methyl group donor for the methylation of phosphatidylethanolamine (1,2-diacyl-sn-glycero-3-phosphoethanolamine, PE) to phosphatidylmonomethylethanolamine (1,2-diacyl-sn-glycero-3-phospho-N-methylethanolamine, PMME), PMME to phosphatidyldimethylethanolamine (1,2-diacyl-sn-glycero-3-phospho-N,N-dimethylethanolamine, PDME), and PDME to phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine, PC), producing S-adenosyl-L-homocysteine in each step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-N,N-dimethylethanolamine + S-adenosyl-L-methionine = a 1,2-diacyl-sn-glycero-3-phosphocholine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32739"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64572"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-N-methylethanolamine + S-adenosyl-L-methionine = a 1,2-diacyl-sn-glycero-3-phospho-N,N-dimethylethanolamine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32735"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64572"/>
            <dbReference type="ChEBI" id="CHEBI:64573"/>
            <dbReference type="EC" id="2.1.1.71"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + S-adenosyl-L-methionine = a 1,2-diacyl-sn-glycero-3-phospho-N-methylethanolamine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11164"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64573"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="EC" id="2.1.1.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="13">Phospholipid metabolism; phosphatidylcholine biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Endoplasmic reticulum membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Mitochondrion membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the class VI-like SAM-binding methyltransferase superfamily. PEMT/PEM2 methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.17" evidence="13"/>
    <dbReference type="EC" id="2.1.1.71" evidence="13"/>
    <dbReference type="EMBL" id="AJWJ01000252">
        <property type="protein sequence ID" value="KAF2072732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSK0"/>
    <dbReference type="OrthoDB" id="16136at2759"/>
    <dbReference type="UniPathway" id="UPA00753"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000773">
        <property type="term" value="F:phosphatidyl-N-methylethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004608">
        <property type="term" value="F:phosphatidylethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006656">
        <property type="term" value="P:phosphatidylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1630:FF:000024">
        <property type="entry name" value="Phosphatidylethanolamine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03216">
        <property type="entry name" value="PLMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004245">
        <property type="entry name" value="DUF229"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024960">
        <property type="entry name" value="PEMT/MFAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007318">
        <property type="entry name" value="Phopholipid_MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10974">
        <property type="entry name" value="FI08016P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10974:SF1">
        <property type="entry name" value="FI08016P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02995">
        <property type="entry name" value="DUF229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04191">
        <property type="entry name" value="PEMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51599">
        <property type="entry name" value="SAM_PEMT_PEM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2 13" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11 13" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="12 13" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Lumenal" evidence="13">
        <location>
            <begin position="1"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="702"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="13">
        <location>
            <begin position="733"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="789"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="13">
        <location>
            <begin position="813"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="13">
        <location>
            <begin position="878"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="796"/>
            <end position="798"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="879"/>
            <end position="880"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2072732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="907" mass="103327" checksum="8139B9DBE2E12FB3" modified="2022-05-25" version="1">MLSSYFKNSNNQIQPTTTKTATVVSLVAFGCLYIANTLYQWSLEGSVDIDWLHHHLPLLGVYLVCSSIFYYIGYFVLLKLCINRWNYNSEKPTHHTLLTTPNASKLVFLLKSDYGFTIPALVLFFSFLFTLSSLTISCDGVSFQRHGKLNLSILAFILLIYLLLTALFYFVVSLKSNAFYQWSTGIDSNLIEFGNGGSTSCAIKEPLVSSFPLSTFLPKLFTYLTNGESTRCVARQKPFSSFDPDGKLTINCPKGVQASYILTPDFFKGNHYLSYKDMNQLIETYAPFERKYNYTKPVIVRDETIVAKCGERSQVHTRNFKKASAQERASHFNRPTLPNENIHIMDQQIKTKPIDIMLLTINGLSRGHFKRALPKTFSTIQAIQGNGKKTSKVYQFSKYHSLWGDSYFNTLSMYTGYNAVRYSEKDIDVKGKSGYAHKWETNPMYYQSFRTDSYVNSWVHGTCADWFTKHLGKTGPTRMDHEFVLPFCSSQVLTPVNSTENYNNNTQNNYPFHIPKRCIDGKHTHSYIFDYLTQFWDNYHDVGKISTVTLMDTFQGTLDINDYVDSQFNTFIKSMQRGHLNDTALFIVGDNGSSLKNYYFNGDDLDQLEISMPALYIILPTWFIDRHPSVNEILKINENKLFTPLQLYYTLRAFSRYAEFGGIDVEDPENDKNIPKGLLDPIQSNITCSDTSIRHDFCQYMAVLLGQFEVTSALLTALLSIVLHVVNYNVTAQFEYKTRFFTKIFGKNTIYYYAVYLVVSALVRDHFINQCIQQDGGIILLGPFEFTELVGNALFIFGILLNIWTLKALGIKGMYNGDSFGWLMDAPVTGGPYQFFSDPQYVGTALACLGTAIRGQSLNGLICTAAVSIVFYISAKHVETPHLNYVYSHRSESKVNFKNLKSLKKMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSQ6</accession>
    <name>A0A8J4PSQ6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BEACH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005923</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000250">
        <property type="protein sequence ID" value="KAF2072757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="18181at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33416">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33416:SF20">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="332"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="568"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="763"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1091"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1569"/>
            <end position="1589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1925"/>
            <end position="2007"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2199"/>
            <end position="2227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2547"/>
            <end position="2567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="771"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1097"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1570"/>
            <end position="1585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1925"/>
            <end position="1948"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1949"/>
            <end position="1962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1963"/>
            <end position="2007"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2202"/>
            <end position="2227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2547"/>
            <end position="2563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2698" mass="306482" checksum="430C0306B55B86C3" modified="2022-05-25" version="1">MEAFRNIKNKFAKHLAARKIRSLWLEYTKVANIEDPSIMVSKLNTFLDSFQETYTELVESGQIKSFDLDRFFGDCSDILVSLLDLVDRESNSNKDNNLDYIVDLINKSLGVAELILHDEKYRLVVKETPNLIYRILTLLDKLNTSENKKVLLRVISAIGENTENRLEIGRLEGFKKLLVLMHNDKELTQEILKTIKHFLDIKQHTSPSSADEELLNNMKKSLSMSSIGEFARHKIGSVVSEGFKVIVGEFQKVFSPNDNEGLVLIPSKILEQSLAHELILSNSFIPSYEMLDKEFSPSSDSELNSNSNNSNSSNSTSILASSAPVSITTTTATAATTTTPSNTPTTASIPINTGSNNNNNNNIFINNSPMSLQTSISENALSESLSINNNNNNNNNNTEEEILKEFMRVQGALASLTDSIREAAQTVQLDLLETISRLLYNNPKNQIEFKNMDGYSILIHIFDNLNDFNNSLQGKEFLEDCFSLFFVIILDGNRLKRITNMDAFDLLFQILLNSSQTKVQLQSLTCIQDLISINPLNIVNIQNMNGIDKLFQLLLILEKKKEEERIIHSNRVNQESETNNKEQQEKEQDDSNNNNNNDNVEEVNNEYYSNSDDYNNVLDQRFDRLQYYNQDYNNQSLQEKELTKLLLATSQLIIYIGVLFSKYNFSVFEKYISLLDPSNTNVHTVGKPMSHTLRCLLLQSTITLLSDTIFHNSLFPINDSFITSFQNTLNTIYDQYSQFLSNSSSGSSGTITTRQMFDNNVKKNQQQDNSNSDDDKDNNNNYDDDDDDDDQEENKAKLSERTLLALELVIMYLRYCPNRQMVNQFLWNQKGIDHFIKLMKLENIDDNVFDLCAWITCDIISSVEMEDLSNPLINSQYTSTINILIQLLDDQKTPNSRKEAICYCICATMDNANTLVYGNHLLKIKLQELGLFTVLIQLLKRLALSSNSTTTIGSQELAYACFATICEVSFGCEENKRVLDIIGYMTLSSLAKSVNVQVDEYLYQLFVELSMNKRVPIRYDYNYLETFFSLDVFKIVSNVLLPQPQYSSILPHSKKHYDINSYNSSSNHNQYQQQQQQQHYYGDEINLGEDGVEFKDSPTSSPSTTPLGSHSLSNSFNGYPQGINNQQQYNLYLQQINSNNATNNANQLIPQPQLIHQQLQQQLKGGIIRIPSFKNLFNGTRRNSIGSWAESFDDLYGLCQEAEHLPAMQPSTTGSSLMNSKTSSYLHLGGLEDEKETIYTYTLLGRSKIRSPDAALMLVDLLPLCPLESQQLNLIFLMKILESNPQNKKMFCSAQGLKFLLYLSHLCPEEIQSYYSRLIVILGLYDISPSEVRLLFDLACFPSFESHPHLMAYKSNTSSAVNSMHNSNSNVDIKRERNRKYELQMQVLYVIGTLTERKAPPLYFNFDGFESGIKLGIIEKFPSARSGYTFSCWMKTGYFLSDESGLFSWQDTLENNIFEVYFKSLPLGQQPQPQQPSQLVETKRYLCIQTRNYPAPPETFSFDKFAFTECGNWYHVVITHSKQKISLFINGKFIQSYSTLNYPKLITKDKPISGWIGKRIYNNSNINVNQQQQQQQQKQPQQPQPSSTAERGYFCGQMGTIHFFEGIWEAPACHKVCSRGNLYNSTYKSLGIDNKEFLVIKPAINSQESGFLQNPSTSIAMSGDIHFSSTIGIGNSNSIASIPTITHSPPLSPSPNVSLSTASTATTNNTLSSLSTTSINPSSTLIKSQSFEETNLLSLSSDITLQQLESSPKKLIVPNETQGATIEFIGGVNVHHTRCLKDVIKEVGGIHLCFPFLDMEPAQQVAGLRILAGLLQKSDSNKAQFRELNGFAILYNLLFKSTTKSSTRSELSMETFDILFDLILDGMNNSLDPKLLWSNDCLLLILDLLAFCSEELQLHVLKYLNTMLLNNPDTIKLWIENNSSSGNTDNSTAPTTSNTTNTSNTTLNGISPTNTSASSQPANSGGVNTNSDSTTSNSNTTNSSSTLNSSVNINNSNSNNNSNNDTTVPISNNSTTISKMNYLLDISLRFSTINTFLQGIIKKMLPHISIEDLESIIEYLTFNKEGSYDHLQTKAKILDLVYEEMRKCPTLLEHLRSIGGFNIVFSLFEIPSEEIHILAIKLTGLLLHSSSKATTLFRRNFGFDVLGTLISRHSPPSIPIGRALVLLMVDEYKFDPKCVAETKQRSSSWISLDFFTRDKSSDSLSTLGSSSSEISEPPVEQSSSSTTNAPIGERVIVYPEALHALMIVLHSFYLNIGLYRSSEYIDLEKIQLALLGEFENLLDPMNMEILWNQPWFEWFYQYIDFTISDSNNNNNNNNNNNNSNQFTYQPLKSLPIYHKLVSICKKMLIHDMCKKQTKILKLKEICIENEDFYINIVASIIDHLKQNSYVLPQQESTDMVKNLVQYFKSLDDMDVPSDISMRIIDSINYIAFHNTPSVRITMKSSGLLEIRDNLIIHLLRGASSRAKFILSFDFECVADQKAFRDSHGILYLLHYFHQSFNDPDTQLVYYLILRNVMAFNSEENRKIIANFFDDQLVTQTFFEDDSINSNGSKSNSGGRNQNNDIEMSNQEDNHFDMTHFINWYYSEELVTKRMEIEARVEKLLHPVEAKHNSHQEKLLKAKEKRIKTRKDKLTKQQIAISKIIQETDEKRKSRSTKMINQYIIKSQLLPLKSQERLKLGRERWDFYKQKLNEICFMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSS0</accession>
    <name>A0A8J4PSS0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PPIase cyclophilin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_006383</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000292">
        <property type="protein sequence ID" value="KAF2072305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSS0"/>
    <dbReference type="OrthoDB" id="271386at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01925">
        <property type="entry name" value="cyclophilin_CeCYP16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000007">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase CWC27 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF6">
        <property type="entry name" value="SPLICEOSOME-ASSOCIATED PROTEIN CWC27 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="4">
        <location>
            <begin position="19"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="202"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="206"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="246"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="269"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="295"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="365"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="399"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2072305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="425" mass="48849" checksum="33CA1EF817A86911" modified="2022-05-25" version="1">MANVYVSEPTTKGKVILKTTLGDLEIELWPKEAPLAVRNFVQLCMEKYYDGCIFHRVIKDFIAQTGDPSGTGTSGQSIYNGEPFKDEFHSRLKFNRRGLVGMASSEPDQNQSQFFITLSKTEELTRKHTLFGRIMGDTLFNILKVNEIEIDPKTDAPLFPPKILSTEIVWNPFEDIVPREKKVEKKIAVEKKKKPTQKNLKLLSFGDDEEENGEEEFTGIKTTLPSLPSSNKSTTKSTTETKPILNKKEEQEEEKEKVETTVNKEPIKKSQQQSTTADIQESKKNLQNQLLNKAVDNDERATELQEKMKQEMKEREKKRQREQLEKGKHNENDDDETLSSSTNNNKQEKKEKRKKVKGSLQFKAKSNEPRRTKAVSETDILNKLNRFKNILETSSSSTKDNKNEEDGWKNHSLKFEKTPGQFLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT95</accession>
    <name>A0A8J4PT95_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006569</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000309">
        <property type="protein sequence ID" value="KAF2072124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT95"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140860">
        <property type="entry name" value="Pseudo ankyrin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2072124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="219" mass="25347" checksum="5D072A505DA03C93" modified="2022-05-25" version="1">MIKTFIDQLPSVFKIASFPIHSRYEHLVEYLVMNGHIGTLNFLINHFNLNLKHQNDNLLVSSIKSGRFHMINYILTLISFKDSKILKSLDIFKKYKESYMSDGSNNSFSYSFSDIDCLPEIFSLVSLRNKCNNCSHSNASNSNGSSSRIGDIQDDYYFNDHFEFKNKSPLSPKQNFNCFTKSNRNNNNSSGESIYNQSEIYSKKDEIEYFLKNIPLSFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUS6</accession>
    <name>A0A8J4PUS6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Disease resistance R13L4/SHOC-2-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_004826</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000178">
        <property type="protein sequence ID" value="KAF2073866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUS6"/>
    <dbReference type="OrthoDB" id="2187496at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF66">
        <property type="entry name" value="FILAMIN_ABP280 REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="5">
        <location>
            <begin position="151"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="3">
        <location>
            <begin position="374"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="409"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="418"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2073866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="493" mass="54705" checksum="13850A090DCD5819" modified="2022-05-25" version="1">MGNNLSQYARDRIYLCKSAGGTDLNLSSCNIKRIPKRILRFKKTLKKLNVGKNLIVEFYSQIEKFQLLETLIAESNELKDTCTQLSKLEHLTVLDVSNNMLTVIPNNLKSLITLNISYNSIQSFGNVTISLPALQELFYSFNKVSIFPTEILEIQTLKKLDISGNHLNYLPDEISNLAPCLEHLNIADNRFTSFPPPISSLGNLRTLNIANNNLGTSLSNQFTTLSKLEILDFSGCSLSSFDFDLSQLQNLTELYLARNSIPYLSPTTSISIGELTQTLRILDLSSGAFTILPKQIGWCTNLRRLNISQNQLPKVPGELHLLNPSIDIIIQPNPLEYPFCEWIKEGIPTFLKNLKPYMRAYGPNCTISDLEPTLKAMAPNQYTINSFDYSNNARVSGGDEFKVQMLLDGSNTGGASGSGEQPSSPDESNGGFPLGRANSMFFRSIECIVKDNKATKPGSYTVYFNSPQTGSYTISITSDNLPIKGSPFTVELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PV26</accession>
    <name>A0A8J4PV26_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTTM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004483</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000159">
        <property type="protein sequence ID" value="KAF2074197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PV26"/>
    <dbReference type="OrthoDB" id="20471at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008488">
        <property type="term" value="F:gamma-glutamyl carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019842">
        <property type="term" value="F:vitamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007782">
        <property type="entry name" value="VKG_COase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12639:SF7">
        <property type="entry name" value="HTTM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12639">
        <property type="entry name" value="VITAMIN K-DEPENDENT GAMMA-CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="280"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="382"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="408"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="606" mass="70348" checksum="7F85144CEBEB7E86" modified="2022-05-25" version="1">MNNSVLPKTYIYFIILLFIIQSYFPLSYYLLPTTDIEQHQQQSIDKSLGSIESLFKKDDERFSWRMFSPTAIDTKCSLDLFYKNNKTNKFTNIKYQDQYFKQWIDLVEMCRTPVIKEIVKDQCIKNQLSQIILIHLGGNFDRFIKTLFGLGDSLINDSTFTMSIVFGIVTSIRCAFQVNSIFEPYLIAVIKAFMVFSSQSDNYQHHYLLVLVLLIIAPIASINVDSLNSKYKVYPFKFLTIQLSIVYFWTFIAKLHSSWFSGQVLPRMLGPEFKRFAQTLFGSTLFIDPMVFISISTVLVELFLVFSLHIEKLRIPTFIFGVSMHIMMGCSGLRIGTFSYFMVIFYFLYIPVVGKRLVCWVINLFRNVSQLLCIDMDRPTFLIAYIGGFGVLSMVLIQMSLPKQFLEFFQIGFSFIIIFILLQALLLIKNQKQLEKEIHVFVLCCLLVCLASRSTPHFRKAFTERATMTGVNNIDLTIANYEIATEILDQEWKYFRLISSPSSEFEFIGDLGIFLESKNNFTKAHTLYNTYYPLYPDQLKLHVGLLRIYQENNDKKSICSMLNEKTCPLAVTISNKPVFSIDDNRNHRFANHVLEICNQLSHSFNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PV42</accession>
    <name>A0A8J4PV42_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_004909</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DOCK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000183">
        <property type="protein sequence ID" value="KAF2073775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="18896at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11684">
        <property type="entry name" value="DHR2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.410:FF:000003">
        <property type="entry name" value="Dedicator of cytokinesis protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.350">
        <property type="entry name" value="Dedicator of cytokinesis N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027007">
        <property type="entry name" value="C2_DOCK-type_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043161">
        <property type="entry name" value="DOCK_C_lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043162">
        <property type="entry name" value="DOCK_C_lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032376">
        <property type="entry name" value="DOCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042455">
        <property type="entry name" value="DOCK_N_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027357">
        <property type="entry name" value="DOCKER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046769">
        <property type="entry name" value="DOCKER_Lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046770">
        <property type="entry name" value="DOCKER_Lobe_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046773">
        <property type="entry name" value="DOCKER_Lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056372">
        <property type="entry name" value="TPR_DOCK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653:SF10">
        <property type="entry name" value="MYOBLAST CITY, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06920">
        <property type="entry name" value="DHR-2_Lobe_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20422">
        <property type="entry name" value="DHR-2_Lobe_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20421">
        <property type="entry name" value="DHR-2_Lobe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14429">
        <property type="entry name" value="DOCK-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16172">
        <property type="entry name" value="DOCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23554">
        <property type="entry name" value="TPR_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51650">
        <property type="entry name" value="C2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51651">
        <property type="entry name" value="DOCKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035232061" description="SH3 domain-containing protein" evidence="9">
        <location>
            <begin position="18"/>
            <end position="1719"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="10">
        <location>
            <begin position="16"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="C2 DOCK-type" evidence="11">
        <location>
            <begin position="542"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="domain" description="DOCKER" evidence="12">
        <location>
            <begin position="1307"/>
            <end position="1719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="194"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="248"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="219"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2073775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1719" mass="196555" checksum="57EACF867C7C2050" modified="2022-05-25" version="1">MSRLFLASAASWKVVASETIAIALFSFTASTIEPHQVSFVVGDAIHLEEETTNWYRGRVLSSGQRGIFPKSYVIIKNTMDFDIVTNELTQVIREWGLLLIQYFKEKKMNEYRVMKDRLSLLLDWHTKINSPSITKEVRDMLKGKVISKIEEGRRLMGLDMIVRTPNGEPAHEKNTGIIQLYRMHHELDNLKLSKFQQQQQQQQQNTLQGKDSGNAYPHSFSSPSLIAPSSTPPSSSFIQATPTFIHASTSSSLGSSSSNIVMGSPTSPSSLLKKLPSSTSLKGSGGLRRSITLRKDVGAAESGGSASGSSSAHLGSYTSSNNLLSHSTSNNGASPNIGSNNYQPNHIFLDLRVFMCAVGEETELYFSLYNRNEGRFITEEFQVGLTAQGMPHDIEKIGKLSTLFIDISKKELQADLYLVCRLIRKGKMLTESNKKAGPLSYRRPFGCSVLKLEDNITAGKEIEHTMPIYTSTQEGQFPLLHEMIIKNPSNLQHVPKAKGIVVGLTLLPGFLKNIVKDNNVLNEVPKTNKLGFPEVIFPGQVRNDIFITLESGEYSQDRKTSAKNVEIVVQARNEAGELLRDALFYGDKSKSEFKSIVFYHSNQPHWSETFRVNVNVNVLDQTYLVFSIRHCTTSENKERVPFAMTYLKLTNPDGTVIANKTHSIPSYKTSKSMEEFLPSLKDPSNKLAIRKGENTKVKVLLCSTLSTQNLSLLTLLKWDQYNGDLAEVLNRFTFVDQIEIIKFMQETFNALFAILEAKKVMDPDLVFQAITWIIGLLVDEKTSRYTNFRPVLDVYIENHFNSSVAHILLIGCLKRILINNNFKTLMSTLKSMEYVLKFIVTSKKNHDKINSTYSHGSFKEDLTSVFNALSTLLKRSEREYIGAQITALKIFSSMFNDLGELFSIEERSEIAKNFVDSVRFDEGFKILNMEKMNVILNLTEGDLFLQATSRKLLVPMVKKHLKQHMSSNFCSSEDTKKHAEIIGVIIESIQSKIKDPQQIWDLVDLLPEIIKSIQTSAWNEFSKLDMIHNLYSILYLMEPEHFDKYMLMNPEVCWKFLLSLLVLLNQLLSTCATYPENWLTLSMFQYSTVKKVIIQVSKYLLDKLKRSSWAQMDQDLWSSFFSLSNTYFKLKGLALENFSEAKQNTIRSRYGDMRNDLIVTIQKMWQSLDRHQIKLLPTVIAPFLELMLINQTNLKQLGIDLYYQLLKCEFKEYRSFKKVETETINTLDAITNSPEASDILDEKFRVFFSTNLDDKMNSDSLIKAQGKVFIKDMTMFLELLYELRTLPDRPEYEDDRTIAAMQLMSYLKQTDRQDTYTKYAHLLCKQHLSNNNFVEAGNTLLLHAELLDWSDDMLEEYGDFRAQTKRERKERLYKQAIEYFDKGKAWENAIALMKQLIVQYEQVMFDYQKLADVLQQESTFYRKVIGVERFFSEYFRVGYYGKGFDSNIQGKEFIYKGYELERMADFVTRIQGKFPSAKLLTYTELPPIEILNSSGQYLQIYAVKPVSGDHIPSSISGTSSPPIPKTYPPAVQKFRSYNNINTFVYSKPFKKNKDKTNNPANEFKDLWIINYYYIIQDSFPTIQRRSEITKRYDIELAPIDNAVNTITGKNVELVEMMTKHENGSESSTSPFTMVLKGVIDAAVNGGVNLYKEVFFKPEYIQENPDKKDSVGKLRSALDNQLIILEKCLIVHGKVCSTEMGGLQEQLEILYRKMRTELEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PV44</accession>
    <name>A0A8J4PV44_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004473</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000158">
        <property type="protein sequence ID" value="KAF2074227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PV44"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038213">
        <property type="entry name" value="IFI6/IFI27-like_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="12981" checksum="180BCE0BA02A3530" modified="2022-05-25" version="1">MSERMKTISNTYFQNAKDLAYTGASKCKQTMKNHPKYTGAVVGTAAAAGVIVTLPIALGAVGFGAAGIASGSLAAGAMANLPVGVIAVAQSVGALGTIAGAVGGTTCAAISAGFGAATGMIASKIIDNSNKTEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVK8</accession>
    <name>A0A8J4PVK8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005134</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000197">
        <property type="protein sequence ID" value="KAF2073549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVK8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140860">
        <property type="entry name" value="Pseudo ankyrin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="703" mass="85195" checksum="00026EB496BDDC6C" modified="2022-05-25" version="1">MNSNNSSHSNKTRLFKLILNNKVIRKRIYYIIWQDYVQQCDYFQKVPYDMFNYPLLWIIKQRNQSLLEYRFKMYKDMVHLRLPSSTNRYYEFPLFSSFCFNALCQWENFPLDLFIEIYYEFLGIDICSLFSTHTIVYLFKYSNHGILKFLYQRNPQIIDFILKTDLRELIETKSFEIFEYLPKFRHYLQTHSIKYILQDNGCYSCYIGIILKLEGQHFFTRDNFLIAIENSRWLYVKEHINRHQLFTFEYTNDDKQQDAWDIKIIQPTQSLNQDKDYLKSIINSSFKSNSLEFVQYFFNHHIEVFKSVLKEYNSIVKNVNVLKYLKSLDSTFKIDSQLVWEYSIQNSDLDLIEYLFQNKYPYNHVGSYPSSNQVVRFLFDNQIVFSFRTNRYDLELFKINENVLYGHEQAIFHAVKYNDVDLFNALIVRLEKDGRYPTFILGYDYWKDLSYTLDISSKIFECIFRLDQNNYFFKSHYHSFQFPYGGMDPTLFQRFYSSKKGNIWTPTPYKTLDIAERFEDRAFQNKFIKDYYECVNRKGSLDFSSERYFQTDIWNDNQITPYIVDLLLHRRLFQSNQLTKARKTEIFVQIVRRAVCKLEYCILSYLYHTNCYEFHNEPQVKKLFHNQSNLNKHVEDFGNFTRNRNESHQAAAILYLQSLRYGFSQIGDVIDRTFTDINFNQLSSTLKYTFHNDQVTIKRETTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVP4</accession>
    <name>A0A8J4PVP4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004157</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000142">
        <property type="protein sequence ID" value="KAF2074530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVP4"/>
    <dbReference type="OrthoDB" id="20987at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="18"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="235" mass="27232" checksum="5DD8D1B98A35B10D" modified="2022-05-25" version="1">MSTSKNLKLKRVNAEINNTFKAAGVLLYSFVKDENTDQLKVKVLIGCEDRRHKPKGGFLFAPFGGKVEQGENAYETAIREFNEETAYLFNDDIQHYNDLIRNPDHTRVFWIPSSKYVLFCTEIDYDDSLSERFLEIDKTGFTNTDQIFIEWVDKNRFLRGPRFSNPIFTRDNGEEGKIASFFISMFGIVDNHLESKLMKRIEQQSTKTTKSRFTRSSSDDELVSNMSLSLNLQDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVX8</accession>
    <name>A0A8J4PVX8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA 3'-terminal phosphate cyclase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002683</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNA 3'-terminal cyclase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000076">
        <property type="protein sequence ID" value="KAF2076023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVX8"/>
    <dbReference type="OrthoDB" id="1911237at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00875">
        <property type="entry name" value="RNA_Cyclase_Class_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013791">
        <property type="entry name" value="RNA3'-term_phos_cycl_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023797">
        <property type="entry name" value="RNA3'_phos_cyclase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037136">
        <property type="entry name" value="RNA3'_phos_cyclase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000228">
        <property type="entry name" value="RNA3'_term_phos_cyc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016443">
        <property type="entry name" value="RNA3'_term_phos_cyc_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020719">
        <property type="entry name" value="RNA3'_term_phos_cycl-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036553">
        <property type="entry name" value="RPTC_insert"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03400">
        <property type="entry name" value="18S_RNA_Rcl1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096">
        <property type="entry name" value="RNA 3' TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096:SF1">
        <property type="entry name" value="RNA 3'-TERMINAL PHOSPHATE CYCLASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01137">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05189">
        <property type="entry name" value="RTC_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005378">
        <property type="entry name" value="RNA3'_term_phos_cycl_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01287">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase" evidence="5">
        <location>
            <begin position="3"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase insert" evidence="6">
        <location>
            <begin position="177"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2076023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="39429" checksum="0888D71E43E99EFB" modified="2022-05-25" version="1">MLKYQGSTHFRQRVICSTLSGRPIRITNIRDQDEKPGLRDYEASFLRLIDKITNGTKIDINVTGTTLTFVPGIIIGGKNISHECGTTRGISYFVEALICLGPFAKVPLDVTLNGITNNDIDLTIDTIRTTTLPVIRKFGLEEGLNIKIIKRGAPPNGGGSVNFKCPIVPSLKAIQLVDEGKIRRIRGIAYATRVSPQFPNRVLDKAKGVLLEFTPDVYISSDHYRGNESGLSPGYGLTLVAETTTGCCLSAECMGGSGEKNETPEELGERAAISLMEEIYNGGCIDSHNQSLALLFMVLCPEDISKIRLGKITPYTIEYIRNLFDFFGVTFKIEPDQDSKTVVFTCLGIGFKNMARTTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWD1</accession>
    <name>A0A8J4PWD1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004597</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000165">
        <property type="protein sequence ID" value="KAF2074112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWD1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF182">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="560" mass="63815" checksum="2F38E7AF25943E53" modified="2022-05-25" version="1">MTKRRLSNDDYFDHLKSDSSFYSIWRNSFIRKNIRNKLCSNIVIDISLDDLDQEYKYLSLFSSQDKIENNILISLTIESKSLLNQYVVHPHKHIINSIWIQPDEENDDYDQYEQEEEKEGEKEQEDIESTFKIFDCNLIHDGVIKFGFHPFYSGVKLLGTLPNSLKEICWQSDGDNFNNTDLDNLLSTNLPIGLETLYLPFHYNIEKDIHLPSSLEKLVYNSNGCKNLEKFIVPPNKTYKDCVPDINSIKDLQWLENQKWVTTISIVSLPCDLKLDLIPNHITCLTLKNEKVLAGGSLPEALESLRCKYIHINGMVLPSNLKHLFVDTWETKTVLPNSLESFTVYSFNQPLQQNSLPPNLKKLELSNYNQDLKVGVLPQSLTRLDLGSFNKPLCKGVLPYGLKELIIQKFNNTLGQDSLPKSITTLEMYAYKGSFQDIGCLENLSTLGILFLCPSISSLLSNVKVKLIFKDISPNLCLSNTSIKDLELVTVGPSQTRTLHTGLLPNGLVRLNVNKIPIHTNDIIPNGCVSLHSTLPIKFDILPPSIKFINNKSIKNYFNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWH2</accession>
    <name>A0A8J4PWH2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004253</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000147">
        <property type="protein sequence ID" value="KAF2074435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWH2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="255" mass="29680" checksum="6720F310F2453ADE" modified="2022-05-25" version="1">MSSSSLSSTSQHQQDNQHQQDNQHQLQQPPLKYWASFYQPPVDKGWDCYLAVNSDSSPNLPPYSRDTHAVYIFFQESKEINNVEYPFIVSFIGYSDRIIDRLLKHYKDPRFYDSSLYCVFPIHIDTNNNTSTTTTTTTSTTIDINKRKKGLRYYNRLKRIFATTIKDDNEDDRYQEEEEEEQEEDQDLEQSIQMGENGMPIDIMKTMLTMTTQSTTNSNKPMRLIQGVPANHPATSVNINDPSYHFPQVLLQQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWP9</accession>
    <name>A0A8J4PWP9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004537</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000163">
        <property type="protein sequence ID" value="KAF2074134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWP9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="54" mass="5109" checksum="D7F770BA76DD50DB" modified="2022-05-25" version="1" fragment="single">QTSAFGGGNTSVFGGGGQSNPSGSVFGQSSSSTSGSVFGQSSGSAFGQNASVWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWW3</accession>
    <name>A0A8J4PWW3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">S60 ribosomal protein L36a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_003491</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL42 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000110">
        <property type="protein sequence ID" value="KAF2075186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWW3"/>
    <dbReference type="OrthoDB" id="2967263at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.80:FF:000001">
        <property type="entry name" value="60S ribosomal protein L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000552">
        <property type="entry name" value="Ribosomal_eL44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053708">
        <property type="entry name" value="Ribosomal_LSU_eL42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10369">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36A/L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00935">
        <property type="entry name" value="Ribosomal_L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01172">
        <property type="entry name" value="RIBOSOMAL_L44E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2075186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="103" mass="11830" checksum="A75DA9B5559E3E96" modified="2022-05-25" version="1">MVNIPKARKTHCVKCNKHTPHKVTQYKAGKPSLFAQGKRRYDRKQSGFGGQTKPVFHKKAKTTKKIVLRMECSCGFKKQQVLKRCKRFELGGEKKKKGEALKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXE5</accession>
    <name>A0A8J4PXE5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 14">6-phosphogluconate dehydrogenase, decarboxylating</fullName>
            <ecNumber evidence="10 14">1.1.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_007419</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10 14">
            <text>6-phospho-D-gluconate + NADP(+) = D-ribulose 5-phosphate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:10116"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="EC" id="1.1.1.44"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10 14">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10 14">Belongs to the 6-phosphogluconate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.44" evidence="10 14"/>
    <dbReference type="EMBL" id="AJWJ01000395">
        <property type="protein sequence ID" value="KAF2071261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXE5"/>
    <dbReference type="OrthoDB" id="434986at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00410"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004616">
        <property type="term" value="F:phosphogluconate dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019521">
        <property type="term" value="P:D-gluconate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000002">
        <property type="entry name" value="6-phosphogluconate dehydrogenase, decarboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.320:FF:000002">
        <property type="entry name" value="6-phosphogluconate dehydrogenase, decarboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000007">
        <property type="entry name" value="6-phosphogluconate dehydrogenase, decarboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.320">
        <property type="entry name" value="6-Phosphogluconate Dehydrogenase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006114">
        <property type="entry name" value="6PGDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006113">
        <property type="entry name" value="6PGDH_Gnd/GntZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006184">
        <property type="entry name" value="6PGdom_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006183">
        <property type="entry name" value="Pgluconate_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00873">
        <property type="entry name" value="gnd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006765">
        <property type="entry name" value="PRK09287.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11811">
        <property type="entry name" value="6-PHOSPHOGLUCONATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00393">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000109">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00076">
        <property type="entry name" value="6PGDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01350">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00461">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0311">Gluconate utilization</keyword>
    <keyword evidence="5 10 14" id="KW-0521">NADP</keyword>
    <keyword evidence="6 10 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 10 14" id="KW-0570">Pentose shunt</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase C-terminal" evidence="15">
        <location>
            <begin position="182"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="11"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="34"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="76"/>
            <end position="78"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <begin position="130"/>
            <end position="132"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <begin position="189"/>
            <end position="190"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="453"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="459"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000109-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000109-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000109-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2071261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="490" mass="53629" checksum="8A3AD9BC54E8099A" modified="2022-05-25" version="1">MTGNADIGLIGLAVMGENLVLNMESRGFTVAVFNRTTAKVDAFVNGRGKGKKFIGCDSIQDFVKSLKKPRKIMMMIHAGSPVDAVIQELLPLIEKGDIIIDGGNSLYTDSDRRVKELEKKGILFVGTGVSGGEEGALKGPSIMPGGNPAAWNSIKPILQAISAKVQPGDQPCCDWVGDGGAGHYVKMVHNGIEYGDMQLISEAYFIMKHYLGLNNQELKDVFAQWNKSELDSYLIEITSDIFGKKDEHGEYVIDEILDSAGQKGTGKWTAMNALDMGIPATLIGESVFARCLSSLKDERVKAAKILPGPNASESSKHFQGDKKQFIEYVKNALFASKLVSYAQGFTMMRHAAKEYGWKLNYGSIALLWRGGCIIRSTFLGKIKEAFDKNPNLENLLSDSWFVDQLKVAQTGWRQVASASLLHGIPTPAFTSALSYYDGYRCPTLSANLIQAQRDYFGGHTYEKLGGKKGEFFHTNWTGRGGNTHSTSYNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXV7</accession>
    <name>A0A8J4PXV7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RNA polymerase II complex component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_001692</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000042">
        <property type="protein sequence ID" value="KAF2076983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXV7"/>
    <dbReference type="OrthoDB" id="343875at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000328">
        <property type="entry name" value="protein CTR9 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031101">
        <property type="entry name" value="Ctr9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14027">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN CTR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14027:SF2">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN CTR9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13174">
        <property type="entry name" value="TPR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13176">
        <property type="entry name" value="TPR_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="210"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="244"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="349"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="536"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="570"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="888"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="912"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="942"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="966"/>
            <end position="979"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2076983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="994" mass="112816" checksum="4824BF6C7FC19CE7" modified="2022-05-25" version="1">MTTAISNKNVSCLYIPIKDYDQGVRVPIDPLPNVDDILEILKGEVAPLDLWLKLAIEYYKQDKIQDFIKLLSQVLDPAIEEFYKDSKVERIAMLNALASYYTQLGTQERDKLKKEEYFNQATFHFNKADKIDLHQPLTWIGKAVLLLCKGEVDRAETNFQKVLDLASKDSTIPGIPAKLGHACILFNKGNYDKALDTYQKVIHQNANCSPSVRLGLGYSYFKLGRNERAKESFERVLQLDQDNVEALIGLSLVLMNEDQLAQAMELILQAYKLSPTNPIVLNHLANHYFFRGELSKVHPLAFAAFNNTDVSHIKAESCYLIARAYHAMEKWKEALQYYHQAVLKCKDFYLAQYGLGQTYLHNGDFDKAIACFELVLEKQPNNYETLQVLGVLYKHSSHSSKDTEKIKRVVKKATELNPTEPSNWIELGQILETTSELTAALDAYERGVGLMKASDQKVSSEILNNIAVLRQQKGLYPEAEATYLQIISESGFELNDYKAVNVTTTYNLARLYESMGQVVKAKEIYKGILKEHPNYIDCYLRLGCICKNEGENYESSEWYREALNISPNNAEAWALYGNLHLSRDEWYHAQKKFEQVLESSNKNDTYASLSLGNIYYNAKFSTPDKVEKYLGNAESYFQRILSKNPNNIYAANGIGMVAAEKGNFALSSEIFLQLREAAIDVQPVSINLAHIYMARGLYDNAIKLYESCLKKSTSLKDTELLLLYLSKAYYEANRFVECKLTLKKALHHSPSNQSLLYNYAITIESHANMISTKPNKKTWDTSNSITELDFAKKVLTNLSNTKPNPKLNFSISKAKHHLAKVEKLHTLLVDIHKNLEQQESDLAKKKGIAFEIQKKEEEEKKKILAEAEALRLQRVEADLKAAQEIQDKINNMATEDDSATTPSKKRKKGNNAEEDANNDDDEGLFGSDDDKKRSKKRKGTDDDGENEQEEEEAAVEGGETGETGEQETNQEGQESNNNEVEMEEKEDLFGSDQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYI9</accession>
    <name>A0A8J4PYI9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003208</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000098">
        <property type="protein sequence ID" value="KAF2075472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYI9"/>
    <dbReference type="OrthoDB" id="20244at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027850">
        <property type="entry name" value="DUF4504"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31366">
        <property type="entry name" value="UPF0739 PROTEIN C1ORF74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31366:SF2">
        <property type="entry name" value="UPF0739 PROTEIN C1ORF74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14953">
        <property type="entry name" value="DUF4504"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2075472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="322" mass="37773" checksum="7C7B61DB0EC9C431" modified="2022-05-25" version="1">MKEIIQQLLSARECKKFQHLFGTALFKSKVKKQWSCFGNANTWIQFIHSLTTLCNRYRSSILIDFATPNEWCDFVDFFHYTITQLSVFMPSLSHLLLIHLDDYLLFIVHKDTLLSRINRDYENDFKHYQFIDVSPKLSNPIKCNSRDLHINQLKIILDIVKKEIENSNSNNSKIKSIDCVQLLDESVMNSIYLPIVSGWFCEYPVIYCNSADCKECSLLNQQQQKREWDDNCLSSCDNLIRFVVYLNTLESGFDKELDKVESLHCLTSFTYPLSLVEQQHIDRFKETIESRLNSNDSNGDMIKVWKNYKVESEIVQLASIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYP8</accession>
    <name>A0A8J4PYP8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Leucine-rich repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_003693</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000119">
        <property type="protein sequence ID" value="KAF2074997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYP8"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027038">
        <property type="entry name" value="RanGap"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113:SF12">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="741"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="819"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="741"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="819"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="838"/>
            <end position="897"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2074997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="897" mass="99567" checksum="B2AF812E47311914" modified="2022-05-25" version="1">MISSSISQQQLNSININHDRLNSFSSSYSSSPSSSPRVFDHQPPITFQQELGNNNIAINNNNNNNNNSNSNNNNNNSIFKNRIIPKLNLNIVNNNNNMNNNNHNFGIMSHYGPATSSPSRKVCKIYRDFCSKYLSASTVNLKLNSSAELISISEALKQKHYINHLNLSHNKSIKDLEAWIAFGMAMKTNLQQNITQLNLSHNSLGNDGLSLIISDIIKNNKTITWLNLSYNNLDQDENHSILYSLQHNQTIQYLDLSGNEYQLSLDISPLLCLFLEKNKSLAYLNFSHNSNRHPLHGLSKSISVNKTLKHLDLSHMTISKEDSIDFVKATISSRNLNSINLAQVFLSNPLMESFGQYITNALSKRYNDFDLEDELGCISFGNNQVVFNIKTLTPPSQVCPQTGDQIMVDNDSNSGSEKEDEEVDDDEYLCTSSPLLHLNLDSINFGKKSLKSFLNILLRNQDLLSLDLSSNQFSEQNGNLLADFIKRNNTITSLNLSNNDFYEKTMDIAEALVFNTSLTYLNLSNTKCSNIIGKILSKSLCLNHSLKKLNISRTKLSYSGILELAQGLGDNRIILESLNLDDTDLGDKGTIQLAEALKTNTTISNLYLNFNSIQSAGAKGLGKSLKSNETLKLLHLGYNQIGIKGIESIAKSLKVNKSLVELSIKNNLVPEKGCITLAESLKGNQKLETLNLRGNFIGEKGGNSILKLLLHNHNLTNIDISHNQLGKEMVVKIYQTLKKNNSTPNINSPSSDSDLHHHSLQHSPMSVRDHHHNPYYHGTINSTSVNPYLYYQTVPSTPRTQTFDDNMMVAMMMDHPQSDCFSSSSSELSPPPTSFIQPSLSHSSSLSTNTTPTSVSTHNLDSPTSLFGSSNRSSLSSDSVGSTSSSVYWSSSSSNFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYU5</accession>
    <name>A0A8J4PYU5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003624</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000116">
        <property type="protein sequence ID" value="KAF2075057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYU5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2075057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1122" mass="133112" checksum="C4F0AE8E4E7F5F3A" modified="2022-05-25" version="1">MNDSNNSFLNLFKNKYIRDQIFSYLKYHRTGYYLFYNSKIHHGLDELSIVDIFKTKNQYIILDALNRYRYTLGRAPIPDPYNINPGSVRVYGESAYYTNYNHWIDNIRIPFDALRDFNDQVKDYIVHQILDLFGTEYVCPHTVLKEFGFSLSRIELLDKYATVKNTCFKYATHNFFQINRDEEVFLWMIKKQRLLVGSSQADFKYLVNQPFFLKNRDSFRLAKEWDDLKFIHFLLIKGLDNPEYQEGVLVDLFRWVISLCPEFNHQPSLANKIKKTTKTGRLYGIQVSIDYEFFQKSLQICRFAFGSVELFQFTIKHFGAHLKSVLSKPHDMRVTVYLNNFEFVKLFCHQEPLYWISNPKMYQIYISDDQNSIALLKKHKLVYKIDGSRPNVTSKQQQELDDFQEFIQLLDQYQPHYLYDQFFINPHQDYDYEYFIKILDSIENTDKEASIIWQALERAYYYLYKATRAQKPNAIKIVELIKRKYFELGEKGHPHPPINHWKLIFLQDPQFYSKLQYDFKEQDPIQCKLIIFKESLKKNEIGMLKYIIDNLDAEQHTAFIKEMPGIMIDFFNEVKRKHIITLKPFFYLSQVFSKHIDIFSIIDQIDIVEKISFESLNTEIIKTMLLSNGGFRFFKGENQEKEIEIQSRIELYFSKLLKLFPDSKSKQAVLLKSFKFKGSSGDRDHVWNLGVTKESIIPLLTVAKQIGYDRFMRFYNSHQDTFRRLVTDLYHCSDYEIFVDVANDCKALIKNTSFQQLFKYCTDPRVFKHYLKEMNVYSIVFSDLDPKILATKPIEFVSVYLERLSQNPNFAILNNPLYDIDTILNLGRMDIAIEYCKWMNPTRVQYTRGTRAKFPVHSLESKYRIFEYFHHNLLSQFPQHPVPKLYAMYFDQSVYKTCACLEFAYSLFGNKMSIDINQQITVFMSKGDERGLQLLHTLKSEGLVLKLRQPTAISAQLMLSTLPAMTLAVEKLGVQTTRLQISDDIHHTDVQCLQYIYHVNPSIEVTPDATNQLFRNASPQVIKYLVLEKNWKFKGSVQYSLLTNDDQVFLFYQSLYQQQNLENQYKFIFVDKNLRNEVLVHYFLTNPIQELKDKILNTLHEKKRSLPPSFKNKLNKRYSLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZA0</accession>
    <name>A0A8J4PZA0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BP74 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002967</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000088">
        <property type="protein sequence ID" value="KAF2075724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZA0"/>
    <dbReference type="OrthoDB" id="2232403at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056422">
        <property type="entry name" value="BP74_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053344">
        <property type="entry name" value="cAMP-inducible_BP74-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35883:SF1">
        <property type="entry name" value="CALMODULIN-BINDING PROTEIN CAM-BP15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35883">
        <property type="entry name" value="CYCLIC AMP-INDUCIBLE PROTEIN BP74-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23621">
        <property type="entry name" value="BP74_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035288685" description="BP74 N-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="BP74 N-terminal" evidence="2">
        <location>
            <begin position="23"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="134" mass="15235" checksum="D684C5266451425B" modified="2022-05-25" version="1">MNKLLIGTLIVFATLLAVASCDEAYFAFKTDGSKHDFVFKLTDAEKIEHARKVLKGEKDQLKIIGRIKKTKKDYNPHYSFHLDPDAIGFFDYAIETCDSSLQYVEDHLDEACGEFLPGCFYCPWSGKLTKEVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZM2</accession>
    <name>A0A8J4PZM2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">SWAP/Surp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_003371</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000104">
        <property type="protein sequence ID" value="KAF2075342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZM2"/>
    <dbReference type="OrthoDB" id="447637at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01800">
        <property type="entry name" value="Ubl_SF3a120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.790:FF:000001">
        <property type="entry name" value="Splicing factor 3a, subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.790">
        <property type="entry name" value="Surp module"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045146">
        <property type="entry name" value="SF3A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022030">
        <property type="entry name" value="SF3A1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035563">
        <property type="entry name" value="SF3As1_ubi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000061">
        <property type="entry name" value="Surp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035967">
        <property type="entry name" value="SWAP/Surp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15316">
        <property type="entry name" value="SPLICEOSOME ASSOCIATED PROTEIN 114/SWAP SPLICING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15316:SF1">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12230">
        <property type="entry name" value="PRP21_like_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01805">
        <property type="entry name" value="Surp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00648">
        <property type="entry name" value="SWAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109905">
        <property type="entry name" value="Surp module (SWAP domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50128">
        <property type="entry name" value="SURP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="SURP motif" evidence="10">
        <location>
            <begin position="16"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="SURP motif" evidence="10">
        <location>
            <begin position="164"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="9">
        <location>
            <begin position="614"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="71"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="440"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="243"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="81"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="124"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="440"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="474"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="520"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="533"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2075342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="698" mass="78733" checksum="18BBDCFD9B2CF0F7" modified="2022-05-25" version="1">MVNEFKTPPDGDVKNRIDKTSEYAARYGDSFETKVLNKEKDNPLFNFMKKGDIYYDYYKFKIQEKKAEKLKQQQEKEAAEKASAANKESANTSTSSPTLAAPAATSTAATVSPTTTTTTTTTPLPQPPTIPTPPPEPKKEIYEPEPLLYILDIPETMSSLELDTIRLTAQFVAKNGQSFLTELSAREAKNTQFDFLKPTSHLYEWFRALVESYVAVIYPPKNIKSTLSSTNFNDKQTILERAINRFEYNQEQENAKKKAEEEEDKEKALIASIDWHDFVIVDTIDFYDEDLDLLPQPKTFEQLISSDLSLANEEDGNNDEEMDVEMETEMETEDMEDGDNDVGAPVQQSKLKIVKDYVKNKAATSTSAAVHRKLTQICQICNQEIPLEEMGEHMRIELIRKNQKERLGNNSNNSTQSTLVEDDEIAKNLQSFASKRSDIFGEDEELKRKEQNRQQSEAPKIIWDGHTNSITRTQQAVHQQQQKIQQQKIQQQQQQQNQQQQQQHPHHQQHHQPPPPPMGFPQQPIIPNQPGLFYPPPPPPMGFGQQAPPPPQFIPGMPQPPPPYNIPGMVAPPGIAMGPPIMQQDMDEPAQKKQKKDDNLIPELLFIQQNPGPVNLMIELSEGDGKASKVFPVTVQLVDLIQTLKEKIKDATGMAPNKQKLKTAGISVLKDQCTVAFYNLKSGSTVSVGLKERGGRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZQ8</accession>
    <name>A0A8J4PZQ8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002136</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000056">
        <property type="protein sequence ID" value="KAF2076585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZQ8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="245" mass="29364" checksum="EC5CB2275B278C71" modified="2022-05-25" version="1">MSYNIQQTKLAFLQAINQMNQTMEHVIYNREEDVDYYQKDFNPQDKRYKDYLQGDLEWGELMFFLYHWNLVQFCANNDYEDDFDNYEEDDDDEEYDQETERENIEYHLEHYNGNNDPNFQIEKDGDGVAPFADAIQRNHVNRLILLFLNDSDTQIPMRLLIEPIQRGQIKILRVLFQHRCFKHMRDRVNFPGLLADILILGDPVLFKIVLAHFRPTLCRELMNRIMAELFPKSSNNNNIMPPLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q033</accession>
    <name>A0A8J4Q033_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GTP cyclohydrolase 1</fullName>
            <ecNumber evidence="3">3.5.4.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">GTP cyclohydrolase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_001430</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GTP cyclohydrolase I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.16" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000034">
        <property type="protein sequence ID" value="KAF2077241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q033"/>
    <dbReference type="OrthoDB" id="4966at2759"/>
    <dbReference type="UniPathway" id="UPA00848">
        <property type="reaction ID" value="UER00151"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003934">
        <property type="term" value="F:GTP cyclohydrolase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00642">
        <property type="entry name" value="GTP_cyclohydro1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.286.10:FF:000003">
        <property type="entry name" value="GTP cyclohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1130.10:FF:000012">
        <property type="entry name" value="GTP cyclohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.286.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00223">
        <property type="entry name" value="FolE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043134">
        <property type="entry name" value="GTP-CH-I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001474">
        <property type="entry name" value="GTP_CycHdrlase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018234">
        <property type="entry name" value="GTP_CycHdrlase_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020602">
        <property type="entry name" value="GTP_CycHdrlase_I_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00063">
        <property type="entry name" value="folE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006825">
        <property type="entry name" value="PRK09347.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006826">
        <property type="entry name" value="PRK09347.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109:SF7">
        <property type="entry name" value="GTP CYCLOHYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109">
        <property type="entry name" value="GTP CYCLOHYDROLASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01227">
        <property type="entry name" value="GTP_cyclohydroI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00859">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00860">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GTP cyclohydrolase I" evidence="10">
        <location>
            <begin position="51"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2077241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="231" mass="26475" checksum="2BC16FD6B5DC097E" modified="2022-05-25" version="1">MSTERDLYNHHHPEENEDEEIYENHKEKQLVDKHVKREKLLDNKQVLGEMQGNVRSLLQNLGEDPDREGLLNTPLRMSKALLFFTQGYEQSVDDVIGNAIFNENHHEMVVVRDIDIFSLCEHHMVPFYGKCHIGYIPDQKVLGLSKLARIAEIFARRLQVQERLTRQIAQAIQTALNPMGVAVVIEASHMCMVMRGVQKPGASTVTSSVCGVFEKDSRTRSEFFSLIKRSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q096</accession>
    <name>A0A8J4Q096_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000095">
        <property type="protein sequence ID" value="KAF2075549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q096"/>
    <dbReference type="OrthoDB" id="10394449at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="497" mass="56386" checksum="F9D5D4B14C2CD82C" modified="2022-05-25" version="1">MLIYLIKDTKVLLDYLNDPHRALINEIFISNSSLNITFPTHNSERSNQQQGQHNNKENSTIVLIDCGVFPESIDKLSIIIHKDTRTCGKLPDSITELSIKKTGYMILDSSNIESILSNLPPSLQSLNLPHSFSITSKCVLPSSLLNLNYQNTYDSLQWFVSPPNRDFSNCRLEISPQQLEYLSSNRSIIGWISSITLKQPSVPITKNMIPSNTKDLVINYADAVIETPDSLPRGLEKLICYERLNIKSINISSMNLKYLTLHFPFDETLEIGFFPPTLEYLTLRDYNHPIKPDVLPSRLKSLVLSAFDHKLLHGALPQSLTKLHLVKFNHQIEPNVLPNNIKNLFLYSFDQTTFKSNALPTSITTLHIPVYSGTFELVDCLDHLQTLSIRSLNQSICRVVRNCKSLELIFDQISSDVKLYNTSIEKLGISNDSGSRINIWPDLLPQFVKSLYLDGMDINSTGLIPNTCTYFRSDISGTQYLSYLPSSVKHIKSLYHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0L4</accession>
    <name>A0A8J4Q0L4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001746</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000043">
        <property type="protein sequence ID" value="KAF2076970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0L4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="590" mass="67875" checksum="8BDEB29C4D8FED60" modified="2022-05-25" version="1">MIIFSDIIISHILNFYVNPTIFPHYFFSSSGPVLVNQITLFNLFNLSLVCKKWARVIVPSLSYPPYTINDPADLMILYRLKEKKISLSDIHLTIEHTDIDVERLRSLQDNVTSLWCQEDALANREFLYYNNCSNIELYYDDEILAKEKDFQEISSVLVKKQHERNTVRPLQSLLISLHNPVSIDEAGLLQFINLMKPKEMHFESTICFTFLKNIGYLARAPCLDQLLMDNITVSLVECGDLIKFNQTIKTISMFEISVENVTNYSQIDVSFINNLLERDKVILCFNLFIKFSKCFATLESFNSLFSNNKTLTSFGCNITASATDKLLAPIKNACVRNLSIMSSNHLIFKNSTFDSLKTLMLNDIDFSFTDTFFKSTLIPSSLTNISIHSLAQIYDKKVDYCLLPFFDSCKNLISFSISGYNESRDQEKLFKDLFESINNHRCIEKLTLYLTFIEEKFINQLIKSNHPSLKNLKVRIKEGTKDFSMILDPLVYNTSLQCCQIENFSNHSQGLNPVDFIIDILNKNKSLVSLKLNYQRTSSIGDYDSKQSTVETLVNSIRHNLTLKSLNLPFWFMDNPKIIKAMASNSIICK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0Q7</accession>
    <name>A0A8J4Q0Q7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Peptidase M8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_000858</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000018">
        <property type="protein sequence ID" value="KAF2077813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0Q7"/>
    <dbReference type="OrthoDB" id="527990at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.132.10:FF:000001">
        <property type="entry name" value="leishmanolysin-like peptidase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.132.10">
        <property type="entry name" value="Leishmanolysin , domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.55.10">
        <property type="entry name" value="Leishmanolysin domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001577">
        <property type="entry name" value="Peptidase_M8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10942">
        <property type="entry name" value="LEISHMANOLYSIN-LIKE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10942:SF0">
        <property type="entry name" value="LEISHMANOLYSIN-LIKE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01457">
        <property type="entry name" value="Peptidase_M8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035275737" description="Peptidase M8" evidence="10">
        <location>
            <begin position="31"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="649"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="412"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601577-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601577-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2077813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="670" mass="74255" checksum="C8B47466019CAE1A" modified="2022-05-25" version="1">MKRYSSSSSSNSRPVSVLCVLALLLAIVAAHSHVTDQSSEIIDHIISKNYLKFARHVDELKSSGEFEQMFDSSSSDGSIDVQLVDGHLPGYDCQHDNIMQTRKIVKPKDNKDMLPRKHYDRLNKQHDDELVQETNANVGPIRINFDTTFLHSKKDPMGCYSTGQKVTFGNGGRGINCSLASEPNCIYTCQANEIFTDALGNLIEQVILPTIRDVFSAFLSVNRVDGNLVLTDTYADRECDNNYRIPDYYFEKGIANTDIVMFITARPTFSSATIATALACSFPIYNNNFFGRPYAAGINFNPYYFKPLLNNPNQFLFKEYIRVGLHEATHALGFSRIFYDSFLNPSTGQKYGYSPSYSFTDSAPTPSGPVSVTRSGIRTPNVVNFVREHYDCASLQHQLLEDAGGQGTAGSHWEKRTVGEEYMLGYVQPVFPITGLTLALLADSGWYAINNTYVEPLMWGRKLGCSWFAGCTATSWSATGYFANSIKDSCTPTRVGKGTVRVYSYDKDLAPEYRHFDNATIGGADYAADYCPYSDVTPNFRQNAYCVDEANQRIASKSIGEYYGSDSRCFEYTSLGSSSQACWKYQCTADKKLQILVKSTWVTCLPGSSPTVDGIAVQCPKNFYICDFNVIPIPGLGNLTMPVDSASSVSSSATLILLSLLSLFSLFILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q129</accession>
    <name>A0A8J4Q129_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001816</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000046">
        <property type="protein sequence ID" value="KAF2076849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q129"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="92" mass="10593" checksum="F0FA4C9ECA41114F" modified="2022-05-25" version="1">MKTYDFFYNERHTTESDISITTSIKFVCPMFDACGFHLALVVDNLYHTGETTSPEELQPPWELLPQQLVSPVPAPRQLQMKTVNSTQLVKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1B6</accession>
    <name>A0A8J4Q1B6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">PHD zinc finger-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_002402</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000066">
        <property type="protein sequence ID" value="KAF2076287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1B6"/>
    <dbReference type="OrthoDB" id="20103at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000852">
        <property type="entry name" value="Histone-lysine N-methyltransferase 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45888">
        <property type="entry name" value="HL01030P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45888:SF4">
        <property type="entry name" value="PHD FINGER PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HMG box" evidence="15">
        <location>
            <begin position="219"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="13">
        <location>
            <begin position="374"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="13">
        <location>
            <begin position="425"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="511"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="10">
        <location>
            <begin position="219"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="309"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="564"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="181"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="269"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="327"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="345"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2076287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="604" mass="70032" checksum="90912C6A2DEB111F" modified="2022-05-25" version="1">MNNQFIPDTPGTYLCSKCGKIKKGHICTAGKQPEPMPQIYNFNNAMSTRRTANQTSQYMQQYTQQQQQQMQQQQEYEMYSFLQLNAISNNPTLNAEQRVAREKAASEFNSYLLLERQERYPFYFDPHSKTIQVISSWEIAVSEEWKHLPEYEKTMINKQNQQMISNRYMYLQQQQDLSEIQKEWQKTQQKEQREMEKYKELKEKRERLIEEAIEKVPKPKSNVSAFFHYMNEFRDEERRNRPDVTFSEISKILSQKWKVLTEEEKQKYHDRAREDKIRHEAEYEKYQERIEEIKLETPPVDLNLVRESAEQANNSTSKSENKEEDSSNSANPAGNASQSSSQAGTEPSDQQPNAEPFTSVSSPHLVSQRSHIQEALCEACQQPDNKINLLSCCSCNKNYHGKCLQLHQVCIEQLKKFNNWKCIDCKVCESCNDSGNEEKMLFCDVCDKGYHTFCLNPPLNKPPTGGWKCSVCVFCSHCGAKTPGSTNNSKWKSNYTCCESCFPQFGDKSYCPVCRKIIKQNNKAPLTKCLYCERSVHNDCDANGIQQHIDSNTTYKCPQCKTGATIKTPKPLIDEDEKKSSKRATSTLGKRKKKDDDDDVNIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1H0</accession>
    <name>A0A8J4Q1H0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002556</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000071">
        <property type="protein sequence ID" value="KAF2076149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1H0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="388" mass="44625" checksum="971BB932B30A9C74" modified="2022-05-25" version="1">MEDLINSDIIYKDPQKWIGTLMGCIPVYGKPLSFVSNYLINFILPSPCSPDGANMECGSGNNGSSGIKLCDFLLEEIENSGSIEIMEKAQQFLKLNSLCLNSKDLFSINRTTEHLNTLVCNNNDTIDCGIQLVEMCINSKLYYPLIISSFTLFTTILETINNGALWKYDDATLVQLRTNLFERAKKVTEKSVVYTKSVLLHYNSTLRSMEGSLFFFRSDYCTVGSFLDPPDKRTILDIIYENQGTLPFTFDKKKCHYIVSPIVFGFQENSYLQSNQYNNNKNMKQQNSNNNNNYSFFTVNFDKKLIGSTWQFRCLSFENCISDIKFMNAKTLEQIAHFEKVENWDPQCKGTINQYYMYEFDKIPLKSNDPIIIKFKNITTGSFEFIIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1P7</accession>
    <name>A0A8J4Q1P7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001032</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000023">
        <property type="protein sequence ID" value="KAF2077645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2441647at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1417"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1471"/>
            <end position="1496"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="3"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="137"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="481"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="735"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1044"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1270"/>
            <end position="1381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1433"/>
            <end position="1449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1476"/>
            <end position="1485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1496" mass="173237" checksum="77F337D6A49B6FA4" modified="2022-05-25" version="1">MNTRELNEKVHNLETEVDNLKKRIKTKTQQYDQQEDLLKVSTKHSNELKNENKKLLSDLRVLEEKFNTCQTSLKQAQDSLKNTKFSQQKLSDLLEQTKSEKTNNEREYHKNMRDLESTTSQQILELSNKCTSTEKQNFSLKLEKDNLESKIISLENNIREFKSQISHCTKEVEQITMEKEEYNQKLIRNMDVERKLSIDLENKELYINDLKVKISELEKEIKDRITELENLSKVQEMVEIQNEEISTYQSLNTELEEKIAQLEETYRSEEQKVKESEILIMDLQCQLSELQDQFKKDEAALKKIISSLEDTNSLLVADRNEIKANLKKEIQQFEDTQDKYELEKSDHLVKIREYEEQIKSLTCTLEVKSSNYSIALDDIVKAKSEIEKLNLSLEIKVREFEIASNDFIENVQSLEKKLHDLQLKYDQQHDLLEEKNKIISEKQSSIGNLHDQLDKASEVESSLLEQLDQSKQSHQELFIKFENIQSSLDNQREKLVATTNELEQAYSNMDSLKKQHQQQIQEMEDRIESMVKESSASSASYQDNINQLKSSIKQFESTNHDLLNKIEDLNVEIATFNKTTIPEKNDLIEKKNKAISTLEEQVAALKTTIQENQDTIHQLDCTVDKKNGQIRQLSESIEQLEESQESSKDLIQKLQTTLDEWKSNCSTLEYEIEQKKITFNDLTCQLDEKQAIIDENQRMMDISDKELVSTRQQISEKSLQLKQSEEACHLQKVENQKLAQDLEQSQTQLAESYQKTTELEKSIHSVKKTFETQGTLQKELEALLDQERYRCKSLQLEIDNLSTLNSTANQHLDHTRQDLAKVNDKLVDKEEAVKNLQSTLDSLKRESFVFEQERNGLLQQVEEFKSLDEKRCATIEKLESSVLELKTNFTKTQDELNFKLSSSNQVIESLKMDIDQLNTEKQSIQENYSKLDQEMKDVSEKINQKDQSLEDYKFKNSSLAQELHQLKEMNSSSNDSVKIELEQAFSTIQQQNQSLNDLNNVHDKLKHEFSLMDYNCKAMESELSSCKQETVELNESIKHHVSCLENEKTRSANLESEIQSQRNEIANLEYQIKGKQSELDSLLATSNSTVQEKQDSEIQLAKTTKLLDEALAKLDSLERDLDGNKKEFTYTEQTIAQLEAHIQELKDKVIAINNELQDEKANNLKLDDRVQSLISENDALCKTNAEKVKEIDSLRSNIEEYKTEQANSQNTQQSLLEKTAALEKKLDDLQQSYQFEKESSHHTAKEMEKDQEGLLEKRTADLEQHHHAEMERIKSQIQTLANENDSHKQDKLDMTHRHKTELDDKSWALKTMQEELDAKKVFINSLEQDIQKMRNENMLLVNTAAAKQDTSATDDNLLDKIKLLTEQNAALKQSLEFKETELNGIFDNYEQLIKKTKLDERKIRLLETKVASLSNLPTAATPKPLQDTTNQLNNASNITTNTSSPSKSTVATAPKPIIGLVKPPLQMLKRKIEFPTDNSKLTKPFQSKPLLKPSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q273</accession>
    <name>A0A8J4Q273_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">LisH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_005805</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000241">
        <property type="protein sequence ID" value="KAF2072881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q273"/>
    <dbReference type="OrthoDB" id="5600002at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45093:SF2">
        <property type="entry name" value="LISH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45093">
        <property type="entry name" value="TRANSCRIPTION ACTIVATOR MSS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="157"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="305"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="497"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="577"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="786"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="157"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="197"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="312"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="376"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="426"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="498"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="587"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="641"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="674"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="798"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="818"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="933"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1066"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1090"/>
            <end position="1100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2072881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1191" mass="129322" checksum="08D819EEA67CD941" modified="2022-05-25" version="1">MTRLENDALNAYILDYLQKNNYQSSANAFKSEVKPHAQVIDSPEGFLFEWWNIFWDIFRAQNGNGISHLGASNELPKQYLEMIKQNQPPAILPGQHHLQQQQHQQQQQQQLAAVAAAQAQAQAQAQAQAQQQGYHLHHSFQQNQFLQQQAQQQAQQQAQQQQQQQQQQHGSQPPAQTPNQINQGAQPQPQPQPQQPPSQAQTQYQGQQNQQPQVQTQPPLQQNNNGNKNVINNPLTGNGNPNINGANSQNNNQQNNNNPNGFFNPPSAGSTPGFNSPPMITPQNLHHFANPQYFQNQGMAQLHHQMGLPYGNNGNPNFQQPSNNNNTNSNSTTPAGNSNNTNNSGNTNNNSGNTNNNNTTSSSSSSAPTTTSSKSNKKEKSSHKDRDTDSSNSSSSNSNSNNSNNDSGSPSTKPSTPRNQPPPPLSNNNNNNNNNNFNANHNVNNPQQPPQPQNMVGISPMGKMFVFGAPMAGAPPSNQFQTLIDQFPAKPVYINAMQQGGQNPTQPQPNPLNTSSPPQQPNPQQLQQQQQQQVQPPQGMPPQYMFFPPQYGQQQYMAQNVGGNNPHGIHYVPTANFQGAIQPPSPINNNTNPTVNNNSPTQIKSEPQPTTPQQHHQQQQEVESKKSKPSQPPSPIKKKSSSTAAASSSTSSTTPTPTPNNSNNTQQQNMTQQEFNNPMSSPINTMGQIALTASGGVPSPIPNQQHLMAFRKPDQQPVMPPQGPQQGMPMGPPMNIGPGGFAAPMNRGWYYPPQGISQYSPGMPLMNPGGQYLQFLPHAFHPNGPPIQSPNVVPGAVMNSSSNPLSTSGGMAPPLSVPTNNASTTTQPSTSSNSTISNNSTPSSSSSSATASSSTSSSSTSSSSSSSSSSKKSSRSSRKNSTTNSEESEKSSSSKLKKRKTSEPSAEPKVAKRNFSKSVPSPQVEHPTPPTTYTPQSPVTPQSPLSSPLNHNIHNNPHINTLNNNINNANLNNNNNNNNNNSNNNNNNNNNNNLNISTGININTSNNNLNNNNNNNNNNQNNPNNPNNNNNQEKQQQQQQQQQQQQQSIIHQQQQQQQQQTPPINSSTNTPAMNPTQGSSLEMNHNTFLNNNNNNNNNNNRGTPQIQQPLQQTGLNSISTSNVGGYDQGSYFELQNFDTVDNQYLKNSPSASQSSNLPMGFPGLSTNSPNFPSKAFGSFEDEDETASFLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2C6</accession>
    <name>A0A8J4Q2C6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000001">
        <property type="protein sequence ID" value="KAF2078668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2C6"/>
    <dbReference type="OrthoDB" id="20663at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="147"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="174"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="240"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="293" mass="32116" checksum="57EBDFE5A1D12E2E" modified="2022-05-25" version="1">MDMKIISSKKIEAKRANEILGQFIVQRKQNEENRLFLTGNAVPTEDFFQVVSSNLSHFQKHLNESIYGPPKEDANGLGGALDVEMIDESSSDERATSIPMDDSYPMTPTLKDSSNQIQISNTADEDGDVSERYSDSSSKKNRRLQLEDSNNNDQSSSNSTETDNEESDDENSSDTDSSNDNNTKSTTAPIKSNVNNNSNNKIKKESSSESESSESSESESSESESESDKKKKSTKKRSSSESSSESDSSSSEDDKKKQKKKVASSSSSSSSESESSSSDSSSSEDDKKKKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2W8</accession>
    <name>A0A8J4Q2W8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Apoptosis inducing factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_001527</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>A + NADH + H(+) = AH2 + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:11356"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FAD-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000037">
        <property type="protein sequence ID" value="KAF2077142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2W8"/>
    <dbReference type="OrthoDB" id="6029at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016174">
        <property type="term" value="F:NAD(P)H oxidase H2O2-forming activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033108">
        <property type="term" value="P:mitochondrial respiratory chain complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012501">
        <property type="term" value="P:programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029324">
        <property type="entry name" value="AIF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050446">
        <property type="entry name" value="FAD-oxidoreductase/Apoptosis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43557">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43557:SF4">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14721">
        <property type="entry name" value="AIF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM01353">
        <property type="entry name" value="AIF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="13">
        <location>
            <begin position="120"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="Mitochondrial apoptosis-inducing factor C-terminal" evidence="14">
        <location>
            <begin position="444"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF14721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2077142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="553" mass="61738" checksum="A3C0DDAFD1BF5861" modified="2022-05-25" version="1">MLRNIIKHQSKFIKRTTSINSNTVFGSANRGFSSSNNRQQQQQQANSNNNYKWNGTKIAGITLLATAAGLLGGISLEEEQTKTDIPVEESTTAPTNKKESEMTEEELRRAKDVEYGIESFKYVIIGGGAAAYHAIDKILENDKNNPTILIISKEYNVPYQRPPLSKNLWASGDNVVEDLEYSDWSGKKQNIFFEPESVYGNEVLNFVRTLKVIDLHVDERLVLLEDGKLIRYEKCLIATGGEPRRLNFSAKNDPNVTTYRTVEDFKNLYNFVGEGNKHITVLGGGFLGSELTCAISQNLGEKKNVKITQVFPESGVLSNVFPDYLSEHATEEIKRSGVQVLNKKLVKDVEKAESGKLVVKTDDGNQWETDHVVVAVGIVPNTNVANSTTLEIDPKNGGYVVNAELQARSNVYVAGDVASYYDFSLNTRRRVEHIDHARATGETAGKNMTGKSEPYTYQPFFWSDLTPGVGFEAVGNTDSKLKTFSVWEKPTDEQKPYNKGVIYYLNDNNVVVGVLCYGNYNKMAAARSLILKKKPVKDLNELQHAISLKEEHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3R6</accession>
    <name>A0A8J4Q3R6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_000495</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat WDR55 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000009">
        <property type="protein sequence ID" value="KAF2078205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3R6"/>
    <dbReference type="OrthoDB" id="2288928at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050505">
        <property type="entry name" value="WDR55/POC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019:SF20">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24796">
        <property type="entry name" value="WDR55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="60"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="103"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="307"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="315"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="331"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2078205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="405" mass="45754" checksum="7769CCF055E742A0" modified="2022-05-25" version="1">MDASSEGGDHDLAPNDILFDTNPFSLNFHPTEDMVVVSDAEGRVKLYKYSVDENETKLSLRPHRSGCRQASFSSDGKYIFTASSDHSMKVIDINTGSILYTREDAHDDAINCLVSRDFMLFTGDDEGTIKVWDMRQQNIVCEFQEHGDYISDISTLDDKHIVATSGDGGVSIYNFVRHSMDDISEKSDNELLSVIPLDNDQKLVCGSQDGTIFIYDRQNLESPKKFVGHPQSVDTLVKVNNQTFFSGSSDGIIRYIGLQPKKLLGIVGEHSQFPVERMALSRDNRYLGSISHDFTLKFWNVSSFYEENDDDEDNSLQQTATGGENSVFKSDDEEDDEDEENEQDEEMDDSDDDSDDDEDEDEDTSSDEDIKPVNKPKKTGPQFTQTQGDRQKKSQQQKKNFYSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3U4</accession>
    <name>A0A8J4Q3U4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GYF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001097</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000024">
        <property type="protein sequence ID" value="KAF2077634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3U4"/>
    <dbReference type="OrthoDB" id="331341at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039905">
        <property type="entry name" value="CD2BP2/Lin1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003169">
        <property type="entry name" value="GYF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035445">
        <property type="entry name" value="GYF-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13138:SF3">
        <property type="entry name" value="CD2 ANTIGEN CYTOPLASMIC TAIL-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13138">
        <property type="entry name" value="PROTEIN LIN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02213">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00444">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55277">
        <property type="entry name" value="GYF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50829">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GYF" evidence="2">
        <location>
            <begin position="404"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="459" mass="53325" checksum="2962B667904B7E34" modified="2022-05-25" version="1">MSEKRKENDDGSNNGDQSQKRVRFNSEIETREIQDHNNNDNNNNNNNNHNHSNNNSNNNNNNISNIDFEQDILKSIERGNQKFKEREYQASRVDKFNKSKSQLEKKRQPKRDDDSDDERVIEGEEDYGDEEHQVLERETRNLLEKRVRAEDSDSDSDRDDEYRSKKGSGASSDTTVEITPFNLRDETEEGSFDKEGNFVHRKANKEDDAWLREYDQVWSAKVPKVSKHELKKENDAQRDEEEDLWEKEAEEQKRKRKLGLTNQEKQTREQHLFNCYSILNSDKNETVLAALRRLGGGKKSGPQVKFKNSKKKADQQAVAMVTEPTKQEAENKFNFDKLTEAADYLLSNGMMDIYSETKDSLKLILEKEKSSLLPSTSPPTTTTTTTTTTTTTNQHDDILLDRNQEQFEYKLSDGNIYGPFPRQTLKEWKDAGYFANHQVLVRNITQSEDDFTLIDLVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4Q3</accession>
    <name>A0A8J4Q4Q3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Dynactin subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_000457</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="13">Subunit of dynactin, a multiprotein complex part of a tripartite complex with dynein and a adapter, such as BICDL1, BICD2 or HOOK3. The dynactin complex is built around ACTR1A/ACTB filament and consists of an actin-related filament composed of a shoulder domain, a pointed end and a barbed end. Its length is defined by its flexible shoulder domain. The soulder is composed of 2 DCTN1 subunits, 4 DCTN2 and 2 DCTN3. The 4 DCNT2 (via N-terminus) bind the ACTR1A filament and act as molecular rulers to determine the length. The pointed end is important for binding dynein-dynactin cargo adapters. Consists of 4 subunits: ACTR10, DCNT4, DCTN5 and DCTN6. The barbed end is composed of a CAPZA1:CAPZB heterodimers, which binds ACTR1A/ACTB filament and dynactin and stabilizes dynactin. Interacts with ATP7B, but not ATP7A, in a copper-dependent manner. Interacts with ANK2; this interaction is required for localization at costameres. Interacts with N4BP2L1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Stress fiber</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Myofibril</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the dynactin subunit 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000008">
        <property type="protein sequence ID" value="KAF2078267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4Q3"/>
    <dbReference type="OrthoDB" id="283815at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005869">
        <property type="term" value="C:dynactin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008603">
        <property type="entry name" value="DCTN4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13034">
        <property type="entry name" value="DYNACTIN P62 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13034:SF2">
        <property type="entry name" value="DYNACTIN SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05502">
        <property type="entry name" value="Dynactin_p62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2078267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="580" mass="66173" checksum="CDACB4AC0F7C86C4" modified="2022-05-25" version="1">MHSIRSNNHCSFNLYNDNNQILKYSCFCGKAFNLTELYYCNGCQQINCRYCITEEIDFHYCPNCIENLNSAEASVNGNKCKKCFECPVCFNTLTYSVIGSKDVNTVTSPPTAGVTSPTTTAATPTTGETYFLNCGFCKWNSLDLKDEFKFDMPSLKAKKESSIQQLQVNKSIEALNKEAAELTSLKDKKTINRIKLAQAMKHFLGPQSKHQKHNQRKNFNKVEDRWESTSMAHPVPHNHLVPTPPPGQTLLSEAAIAQRVQPITMTEADQMQQQRIDAREFETLDPLGDTVKYDDDITPFLRDQVTECDQVSSLDQRFKNIAHSSLSTSSIHPQRKHLITKRSKRCKRCDKLLVKSEISLTKTEFKRQHFAFTYVPRVQITRVLWIDNDIFEVFLQFTNPLHIPLFINFPMSIKGSFISTDDNSQPIDLCEHETYINGILDETDDLEKESIIQTLKQNENPRYIIDRKDNKLTMKFIGKVGIEEEQIAPKDNNNNEEITNLTIIHLTESLANLSTSTEIPKFNSQFKDIKFTITLEFSTSSLSTAPSTSSSTTTADKQQKTLLKLLFDIPSNKFENTLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q679</accession>
    <name>A0A8J4Q679_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GTP-binding nuclear protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_003944</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the small GTPase superfamily. Ran family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000131">
        <property type="protein sequence ID" value="KAF2074756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q679"/>
    <dbReference type="OrthoDB" id="48625at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00877">
        <property type="entry name" value="Ran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000369">
        <property type="entry name" value="GTP-binding nuclear protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002041">
        <property type="entry name" value="Ran_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24071:SF0">
        <property type="entry name" value="GTP-BINDING NUCLEAR PROTEIN RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24071">
        <property type="entry name" value="RAN GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00627">
        <property type="entry name" value="GTPRANTC4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51418">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2074756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="215" mass="24081" checksum="50DA4906BC265C46" modified="2022-05-25" version="1">MAEIPPVKLVLVGDGGVGKTTFVQRHISGEFETRYIPTLGVSVKTIAFYTNYGKFIFNIWDTAGQEKFGGLRDGYYIQGQCAIIMFDVTSRLSYKNCPNWYSDLTRVCGTEIPIVLTGNKVDVKDRKVKPSQINFHRRFNLSYYDISAKSNYNFEKPFIWLASKLCGHKDLALVQEPTLAAPEVALDKQQMSQYEAEVAAASAVALPISDETDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q6K4</accession>
    <name>A0A8J4Q6K4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003623</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000116">
        <property type="protein sequence ID" value="KAF2075056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q6K4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2075056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="704" mass="83153" checksum="9D8583EE39920F6F" modified="2022-05-25" version="1">MEDQNNSRSNNDNHSSTTNHFFSIFRNKPLNQMVMEVISKNINISYDFYRLPLHLVIANKRYDYLVYKIDKYLEWRASNHATEWYLELSKHDIKPLFSVAHLIGYDKFMVLYKDFESLFDTVLLKNDYEICSTDFKVFQHFYKLYQKESFSNFEKFLSNITEITNVEVFEALMINISKHPIPTLRNISKEFLINLDTKPQLIKVFCQLLATWVSPKYNFNNDVNNLINFELTQLAREYIYIIPKNTYFVWEKLFRPYPKSLRKKYETFQIIFQHNPNYFKTYGTDGDSIFNRFYIPHELYLSSRDCLDFAIARKGSDFKLDDTSRVWISDAFMTKGDMDGVLLLHKLDLFKFIFDVDTTLPILKFCIEFYNLKLNTVCARGTQPSFVGDFECYKYIYEKFPSLSANPFLFPHLAKCKTASKKLTRLLVENTTKISIHTLTLALLNNNFQLFKILWENKEEKFKDQNIESLNSGLMYIFNQQQTDTLEYFLKDPILAPILIKYVNATASSIPRCQLEKLTKYYFQLDVKLLPSQYSISQCFTKNMTNIILYKIATNSYSDHHIRVVINKSLNLSYFNQFKKLLTGIIDHQPTINDKSKIIFIILFSSIKRGLIDKLIQLFSNPYPSIKLENIEQNFFSLSPGELLSLQKDLDSLLPDQKNYLIKIGFYKNLNNQTNIFSFNSNTLDSHNSKKRKENTSLNELFHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q887</accession>
    <name>A0A8J4Q887_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_002155</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CSN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000057">
        <property type="protein sequence ID" value="KAF2076541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q887"/>
    <dbReference type="OrthoDB" id="422427at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048624">
        <property type="entry name" value="CSN1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019585">
        <property type="entry name" value="Rpn7/CSN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045135">
        <property type="entry name" value="Rpn7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145">
        <property type="entry name" value="26S PROTESOME SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145:SF2">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21151">
        <property type="entry name" value="CSN1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10602">
        <property type="entry name" value="RPN7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="8">
        <location>
            <begin position="214"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2076541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="457" mass="52008" checksum="094BEFF769ED8039" modified="2022-05-25" version="1">MEDDMVIEVAGSSNGEKTTTTSSLSSSEFDLENYINNYSGNTKINRLIFIAEHCKPLEAEAYQMAAKEVQKTPNYELYKTIVGKSHGILSLDSAWVDNMAKKNLAQLDKLDSDLNTAKANMVKDSIRLAQNELGEFYYKSGDYPNAMKSFVRTRDYCTTSKNILTMCFNIIKVGIENVNYSHVITYVTKAEQSPDLDNASRAKLNSCMGLANLHLSKYKPAAKKFIETPFEQANVFSDVLSQQDIAIYGGLCALATFDRSELKKKVIDDPTFKQFMELVPEVRELINDFYNSKYGSCLSYLDKLKPSLLLDVHLFEHVEVLYSKIRSKSLIQYFSPYVSVDLNVMATAFNTTVQSLEKEISKLIMDDSIQARIDSHNKRLYARKTDQRTSTFEKTIQVGNDFQNSTNNLLLRLEMLNNNITTTSGRNKNNNGMDFEKSQFLENFHPQHSHFFQSHGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4QBI5</accession>
    <name>A0A8J4QBI5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Store-operated calcium entry-associated regulatory factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000161</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000003">
        <property type="protein sequence ID" value="KAF2078536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4QBI5"/>
    <dbReference type="OrthoDB" id="17636at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035288687" description="Store-operated calcium entry-associated regulatory factor" evidence="2">
        <location>
            <begin position="24"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="215" mass="24533" checksum="639EFF7FDD14A4DE" modified="2022-05-25" version="1">MKLYILQLLLVLSLLLCIDNVESSFVVNDDVYGGGRDSGLPILRFSKNMLTPNERHQLICIGGTARGTNEEAEYLICKSSMNDIYHKDVEWKCQSSTISKNVRLTKLNIDCPKLNVDYTYGDDHPCNLEYHLDWVDNYTFSKKAGWGILGVFFFYIFLKRNTNLYSGQISNPIFLPREQINPKVFRALGIVVSIIVFISTIVFILLCLLMFSAPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UP55</accession>
    <name>A0A8J4UP55_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_009945</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000862">
        <property type="protein sequence ID" value="KAF2068731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UP55"/>
    <dbReference type="OrthoDB" id="19313at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097039">
        <property type="term" value="P:protein linear polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23820">
        <property type="entry name" value="RWD_RNF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051628">
        <property type="entry name" value="LUBAC_E3_Ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770:SF13">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770">
        <property type="entry name" value="UBIQUITIN CONJUGATING ENZYME 7 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26200">
        <property type="entry name" value="Rcat_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RWD" evidence="9">
        <location>
            <begin position="13"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="224"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="58"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="363"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="364"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2068731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="568" mass="65799" checksum="367235EED0949914" modified="2022-05-25" version="1">MDDLEYCKEIQNQEIEVISNIFIDNFTTFYDYDGVIGKYNLRVQVDVPENFTITSYNLKENSDSDNSDNSGTSDDSDGDVKQHKHDHHQQQQQQQSYAIKYLPYIDLSWEYPAEYPEQPPRVYISSIWLTLEQMFGIVKKLESDWTSGEMVIYKYYDWIKTELLSYLQIDKTQVFTNQQVDLESPLCSIVTWTHKNEWDSLISTLPFLISYSKNQEKSQFALAESYECPICCCDYAPDEMVLLECLHYSCKDCTTQICKVNIESGSIKLLKCSNLECNQLLDQDFIKSIVTPAEYKQYEVFLQQDRNLVQCKRCPTGWGYLDNLTKSSYCTQCKYCWCTLCHSDIHYGLPCYYVAPPKVRVQRQKKDKDKKHNDPEWLNRPPAPPPVSIVKSICKKCPGCGALLDKFEGCNKIVCPYCKRMLCWLCLSTITSYEHFQKSCVLFETPLAEPAVPLPPPPPVIDKEKARTKDISNCGKCKNLIFRNNNNNHSYCDPCKQSVCFLCKEFIKGTTHFTTSNCIQHGDPPNRVVDEAFISNQINRFNNPKPQKTLRKGIPTMKNVIFYDNLHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPX5</accession>
    <name>A0A8J4UPX5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008811</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000582">
        <property type="protein sequence ID" value="KAF2069871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UPX5"/>
    <dbReference type="OrthoDB" id="427795at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10971">
        <property type="entry name" value="MRNA EXPORT FACTOR AND BUB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="120"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2069871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="37436" checksum="B78249E9CB6C01A2" modified="2022-05-25" version="1">MSNDNDQDVLCNYVSQRLTSSPYDLKWIPSSPCILTVGKKTVGGVVKGDLKIHKFEYIDDKPKLSLEYEGNSISPLRCCSFMRSSTTINNNERIFVTGDFSGSLLIWDSDKINEPMASNSNTHQGTISSIDTHLESNIIVTGGKDSLVCLWDKRDIKSPIYKYTCQTKENSNCWAISTNGKEIICGYENGQLNIFDIKTNSLKANTRLKGGISSIDISSINNVQDIGNEFLVSTSQSLICKMKLQQDKLSIVKEITTDSEMVWSSKYFNNHPSIYTLMQGDGNAYIYKNDQLLKKLSISKNSPIISFDHNPEKLGLMSCITLNKQLFVLLSPLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQ26</accession>
    <name>A0A8J4UQ26_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Biogenesis of lysosome-related organelles complex 1 subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008607</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BLOC1S2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000546">
        <property type="protein sequence ID" value="KAF2070081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQ26"/>
    <dbReference type="OrthoDB" id="244061at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031083">
        <property type="term" value="C:BLOC-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099078">
        <property type="term" value="C:BORC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032418">
        <property type="term" value="P:lysosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019269">
        <property type="entry name" value="BLOC1_su2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46479">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46479:SF1">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10046">
        <property type="entry name" value="BLOC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="147"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="185" mass="21226" checksum="193C9610D730F232" modified="2022-05-25" version="1">MSDKSNINNDTIQQKVNNDILKPNLPVTEDLQHMTGEMFTKVTNYLNGELSTTVADYNLLIQMNNVTAAKYQDMTNQTKGLSLFMSDLKSKYEEFQPYLDKISDLDKNVTDLEKTVLLLDEYTKRLETKIKNIDKSSLLPIKEKQQQIQQQQQQQQQEALKVQQQSSTSTTVADSTPVEKIEESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UU38</accession>
    <name>A0A8J4UU38_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DDHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003668</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000118">
        <property type="protein sequence ID" value="KAF2075016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UU38"/>
    <dbReference type="OrthoDB" id="69269at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004177">
        <property type="entry name" value="DDHD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058055">
        <property type="entry name" value="PA-PLA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23509:SF8">
        <property type="entry name" value="DDHD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23509">
        <property type="entry name" value="PA-PL1 PHOSPHOLIPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02862">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01127">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51043">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDHD" evidence="2">
        <location>
            <begin position="248"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="326"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="422"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="455"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="214"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="334"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="423"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="455"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="488"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="551" mass="60549" checksum="F988D66D6FF84AB6" modified="2022-05-25" version="1">MANNSNISINNNDDCETIQNIDHFILIIHGIGKHEENWVAKINKINNLYHTVTKIIGYNKNIKFEGIEWHSALHSHTDRFIEKVTPPYITKVHEMINHTLLDILFFTSPIFSQTIYNEVGKQLNDAYNNFILKNPNFNGKVSILAHSLGSMICYDILCNQPISSNDNNNNSNNNNNNNSNNCNNTSTDSNNDGDDGAEKGNVYYWQKLNQEKQSNNNSNNSNSNNNEEQDIGDTIMGSLMPAIKFPKLDFEVYNLFGIGSPIGVMCTIRGHTALDVPKCVNFFNIYDQSDPVAYLIEPLIDDGFSQLPESVAPHLGSKKSFLKQQEKLEKQNNSTTTTTTISSSTSSTSLSSLSISTSPSSPSTASSGSILSKFANKLPTLRSLAGGSKSSSTTSPIQQSPNSLGITCVVEQTEMVALDGASTPKSLKHQNNASELNPEDNLPIIKLDSIDSTTSTYTSTTTTTTTTTSIPKSRSFDASGGESEEETPNTTNSSTATTNTNNNKFCSGHRFDYKLKPTGFSNISEYSSVPTAHKSYWESKDVLYFIISNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UUM3</accession>
    <name>A0A8J4UUM3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003069</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000091">
        <property type="protein sequence ID" value="KAF2075646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UUM3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2075646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="167" mass="19136" checksum="CEF1CA5C64498399" modified="2022-05-25" version="1">MDQFEYLASPSISSGFTLSPTNTNSSKYQQEEEELLLEQDWIMLNEKSPFLSPSSFYSQNNNDSLEQDLPLIQELLNDVQHLEIEDNFEKDADEPLYKNNSLILTPEFLLSLNVFSQNMSWIANQVTFKCIQQSQATSNKIKSLGAIIKNQCKAISSLSYNNYLFEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UUN5</accession>
    <name>A0A8J4UUN5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rab GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003004</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000089">
        <property type="protein sequence ID" value="KAF2075691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UUN5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001447">
        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="23463" checksum="0EF91E25AB5455F1" modified="2022-05-25" version="1">MQEREKERVYLKFIFLGASKSGKTSIIRRYIENKYSEHLYYTATIPADYRTRFIQDIDPNKTLFLQVWDKYTGERFGFIPPDFFKYVNASFIVFDITNRDSFNYVKEEITRIPHDEPNQYRELYIVGNKSDLESHRVINRDEANQLAQDNAAKYIEVSAKDNINVYQSFDEIAKSLFFKKINDPSSFPPSPPSSFCTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UV53</accession>
    <name>A0A8J4UV53_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PWWP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010364</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001064">
        <property type="protein sequence ID" value="KAF2068310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UV53"/>
    <dbReference type="OrthoDB" id="21615at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05162">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2670">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042065">
        <property type="entry name" value="E3_ELL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWWP" evidence="2">
        <location>
            <begin position="19"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="348"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="479"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="597"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="164"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="200"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="300"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="352"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="481"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="627" mass="71579" checksum="5DEC371EEDE956EE" modified="2022-05-25" version="1" fragment="single">MTIDHEPGYVLSASYGTWGVWPVRIAKSTEVSPATLKSSKKDNTLVYFFGSHDYGFIKDTQFRPYEPEELLKQKFKARDVKNLHKAIEETNEWVKCSKKEYPIYIPLVKTKKELDIDYCQQKKKKTNSQSSSQSNYTVINNSNQSNNDINLLTSPPSKIITRQSSIPTQTDTSSSSSTANTNHNNSINTNISDNNEENKSNNTSTVNNKNNENNSNAKIKEEQHFFSSDENNINYQNTLAITPIKPSISSLTHGKPSKLTFSPLLSSVQKEPQQITKSVQKRRQADQILNSPSPIKFISNKKSNNNNNNNNNNKNNNNNSNDEPQNLFLKFSTVTQAITHSTQDLFSDISENSSNNNNSISNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNININTLQRDLIHLLALHSLSSSELKSKYDIEDDINLDKVLEKFAILNEFNQWVLLQETYIFINEDWYKTEIEKKTLRKRKLEFDNNNNNNNNNNNNNNNNNNNNNNNNNNNINGSNSSNNNSIIINNNNYSTPNNKTTPKNINKKQLVDSPLSQRESTTKKKKLDKKPNCYMDQQDDLDITLPLLDTTHFLDENDININNNNNINNNNNNNNNNNNNNNNNNNNNNNNNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UV90</accession>
    <name>A0A8J4UV90_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_010259</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001001">
        <property type="protein sequence ID" value="KAF2068415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UV90"/>
    <dbReference type="OrthoDB" id="8057740at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="3">
        <location>
            <begin position="158"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase/retrotransposon-derived protein RNase H-like" evidence="4">
        <location>
            <begin position="278"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase zinc-binding" evidence="5">
        <location>
            <begin position="411"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="14"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2068415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="672" mass="78142" checksum="7C0FEB13F781DD26" modified="2022-05-25" version="1" fragment="single">KVQEVEQNILTETESPKEKRPSTAIDKPKVDASIAATTTNTEDKNRNNNSVKQPEIERKSAKSKQSGSTSAKGDDYTAKEKFRLTKLAKSLKRDLDKETILDAKDIEQISKHIDQIMNVYKETQFQSPKREGFYMAIEFIDDKVPPPFHNRYPNKNKEDTMKNQTCTRGRCNYRSYFNHWVQELLRQFQEFVVAYVDDIVIFSNTLEEDYKHVKKVLDLFQKNRQKIQLFKTEVKYVGHMVSRNGIKPIYDKTKAIEEIPMPSTVSSPTSSKKIELPEKQQSDVNAIKAALTSPDTLIAIDFSKRFYLYTDVSDVGTGLMITQRDKVIYDSKKLDKHRKRYDTRDRELLAIERWSELETGREWDQDFVTQVVESYEHHKKKEWLQALIRRDDVVKDNDGLLYLVDKGKILVDNKQIETILQEAHATTFGGHVGKNRLAARLKDLYFFKWFWTVIEKFIKACPECQKCRIETVKQGFLNPLPIPDRPWKDISMDYIKLAFLKDGAPQTIVSDQDSIFPSSLWKKMMFQNVNDSTRPCSSRWSNGKNRKKWCSKIHIIEASINGFSNVNSSTGRPYPNTAKFSIDTDLAKEKKLLPLYCGPFKVIQKSTKNNVNYKIQIANSRKQSRTVHIVDTKLYIQDDKYFRPANKKVHPILSQEIEKIVSHREVSNSSQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVQ8</accession>
    <name>A0A8J4UVQ8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Integrase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_009754</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000796">
        <property type="protein sequence ID" value="KAF2068925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVQ8"/>
    <dbReference type="OrthoDB" id="10063667at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase/retrotransposon-derived protein RNase H-like" evidence="2">
        <location>
            <begin position="97"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase zinc-binding" evidence="3">
        <location>
            <begin position="196"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2068925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="293" mass="34103" checksum="8D0A6CA87C2AD046" modified="2022-05-25" version="1" fragment="single">VVAFVDDNVIFSNTLEEHYEHVKKVLDLFQMSVKRRSAIQNRGICWTYGIKRRYQTNLRQNKSHRGNIPMPTTVREVLCHVGQPRHRKRKLSSLKSNSAIKASITSADTLMAIDFSKRFNLYTDASDVGTGLMLTQRDKGGVETKWDQDFVTQVVESYEHHKKKEWLQALIRRDDVVKDNDGLLYLVDKAKRLILVDDKQLETILQEAHATTFCGHVGKNRLAARLKDLYFFKRFWTVIEKFIKACPECQKCRIETVKQGFLNPLPIPDRPWKTSRWTISNYQSPTKAKIIYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UW99</accession>
    <name>A0A8J4UW99_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycoside hydrolase family 5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009202</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000654">
        <property type="protein sequence ID" value="KAF2069480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UW99"/>
    <dbReference type="OrthoDB" id="19354at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051923">
        <property type="entry name" value="Glycosyl_Hydrolase_39"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12631">
        <property type="entry name" value="ALPHA-L-IDURONIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12631:SF10">
        <property type="entry name" value="BETA-XYLOSIDASE-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035166878" description="Glycoside hydrolase family 5 domain-containing protein" evidence="2">
        <location>
            <begin position="28"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="465"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="469"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="547" mass="60582" checksum="509B65C9E48EA8A0" modified="2022-05-25" version="1">MVIEKRQLYVLLVVVVLSCTLVRDANADSVPKALIDTPGKTIDFSQFLGIGAQLEWFNATVVQKQIDYLKAINVSWIRVDVHFDQLVPSQGNYQWGKIDATLQVAKNNNMNVIVFVAGTARWLSTYYDNNTYNLTFTDTWPPSNSTLYGEILVTMNARYPFVKYWELWNEMNLVPAFWAPTYNPTAYYSLFAEVKRVFTLYNIQDKLIPGSMGYYGNTDVQDQNMIYDLVSAGNPYKNSICSYHPYTEYPEGGPTQLPDSMYGNRNTTAYLNYVLKSQASVSQIWATEWGWSTHDVSELTQAQNTLKRLLMDTINGLDKTFLFTTSDLDARASSLRDQKYGIVDLNLQPKPVYTALKRLLITILSSSSIGPCKSELNFVGTDPNMVFISFTKLSTNTSMIAYWNANISAPTSTITVSGANISPSSKARVYQLTQDPNSDISSSSSSFFSLQVQHNIQIIEWPIKDDDSSASPTSSSSSSSSSSPPATSGSTTTTSSTSSTSTSTTTTTSSPTTSKSNDPDSPSNSSKNNTPILLLFFSFIGVLVFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWP5</accession>
    <name>A0A8J4UWP5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008749</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000571">
        <property type="protein sequence ID" value="KAF2069930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWP5"/>
    <dbReference type="OrthoDB" id="17470at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045068">
        <property type="entry name" value="BACURD1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING ADAPTER FOR CUL3-MEDIATED RHOA DEGRADATION PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145:SF8">
        <property type="entry name" value="RE57120P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="192"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="160"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="359" mass="40638" checksum="70B0B0839B9FD533" modified="2022-05-25" version="1">MNPNTKKRAEISQSIKGLKGLINGHIESLEQQVGELEIRRDALLNVAKVIDVSVIPDPITLNIGGFKHQTTKATLTKIPNSFFNLMLSGEIDIKPMTNKPNTYFIDRDGTHFNYILNYLRDEGDIQIPEDIRYCVRKEMEFYRINNHFKLDDTTTIIDQLNSLKLKLDETEQELVLTKEESKKELDNLKQELALTKEEGNMTTKSLKQEFHSFKELIFHIASETNKFSASAFHSACDGKGPTITVIETTDGCVFGGYNSQSWKIHGYFYGDNKCFIFTLVNKHGIPPTKYLPKENANCVGSNSDPVIGFHSGNNDIRIGETSSYQVFPSTYKDTTGKGNTTLTPSKNFTIKNLEIYKCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXC1</accession>
    <name>A0A8J4UXC1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">COMM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000303</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Scaffold protein in the commander complex that is essential for endosomal recycling of transmembrane cargos; the commander complex is composed of the CCC subcomplex and the retriever subcomplex.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000005">
        <property type="protein sequence ID" value="KAF2078436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXC1"/>
    <dbReference type="OrthoDB" id="64318at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017920">
        <property type="entry name" value="COMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037360">
        <property type="entry name" value="COMMD9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15663">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15663:SF4">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07258">
        <property type="entry name" value="COMM_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51269">
        <property type="entry name" value="COMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COMM" evidence="2">
        <location>
            <begin position="13"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00093300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="92" mass="10569" checksum="711E856DD6522B5C" modified="2022-05-25" version="1">MSNQSTTSCSLPKIIDMDWRIDIKSSSNLVNTMNVPTVLFKLEVEDQNKKVTKDEDDDEKKTKTVIFELNKETINTMLDGLEFVKNQLDSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXR4</accession>
    <name>A0A8J4UXR4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endoplasmic reticulum junction formation protein lunapark</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_007356</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in determining ER morphology.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The C4-type zinc finger motif is necessary both for its ER three-way tubular junction localization and formation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the lunapark family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000387">
        <property type="protein sequence ID" value="KAF2071325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXR4"/>
    <dbReference type="OrthoDB" id="20953at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098826">
        <property type="term" value="C:endoplasmic reticulum tubular network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071788">
        <property type="term" value="P:endoplasmic reticulum tubular network maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903373">
        <property type="term" value="P:positive regulation of endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040115">
        <property type="entry name" value="Lnp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019273">
        <property type="entry name" value="Lunapark_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22166">
        <property type="entry name" value="ENDOPLASMIC RETICULUM JUNCTION FORMATION PROTEIN LUNAPARK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22166:SF12">
        <property type="entry name" value="ENDOPLASMIC RETICULUM JUNCTION FORMATION PROTEIN LUNAPARK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10058">
        <property type="entry name" value="Zn_ribbon_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Lunapark zinc ribbon" evidence="3">
        <location>
            <begin position="274"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="143"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="326"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="143"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="183"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="208"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="252"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="330"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="383"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="397"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="411"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2071325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="423" mass="47847" checksum="30A383620CA7BFF2" modified="2022-05-25" version="1">MGILFSKKNGFAKQLLDIEAKINALEDKVSDSRRFQQKALHKIIVYSFFIEFVIVVFTYMKVRQFELLSQKLYYSIFVVLFPLIVFIFTKIFGVVYRLLIARNEKKLESLKISLQKKLEERKKETDFEATQQLLDKYEKMITNKQGQSPKNNSQVTPSLSNSGSGSSSSRNSSQTRQRGQPRPSIQPQQQNVDSPPLNKQTGSRPSLTPSQQQQIVQQQQQQQQVNRTNNNTNTINNNNNNSSPSSPSQQQPLPPQPYTPPPQQQQKPNTKRWWLDKLVDYLISDGPNYGSPLICSYCHSHNGYVPVEELSNIQFRCSYCKTFNQKEDNNNNSSNATIKNNNSSSTSSSSLANNNNNNNNNNNNNEGSIDKVNDLQPPIQHISLEKSENKEGDDGGSSNSTTANTNTPTKKSSKSPNKNKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYI7</accession>
    <name>A0A8J4UYI7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA polymerase alpha subunit B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_005832</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase alpha subunit B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000243">
        <property type="protein sequence ID" value="KAF2072855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYI7"/>
    <dbReference type="OrthoDB" id="336885at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016722">
        <property type="entry name" value="DNA_pol_alpha_bsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23061">
        <property type="entry name" value="DNA POLYMERASE 2 ALPHA 70 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23061:SF12">
        <property type="entry name" value="DNA POLYMERASE ALPHA SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="7">
        <location>
            <begin position="370"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="198"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="198"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="211"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2072855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="624" mass="70607" checksum="2C1AC44752FEC86E" modified="2022-05-25" version="1">MANLVHIGIYLKEANFLGFDHDDLRNVQKIMEELGLNETVVSREWDLYALEHKIHSNFSQIIPKFRVYLKRRAKGLLEEIRQKELEEQDGIFDDVPLSLVVDRVTSESSGVKASSPVKSSPAKSTTAAGTTTTATKKEDDFDFDFNDNSGTRKRLGNPTSKTFVYSNRSHVGQVVLKYNQDIPTGTMETKPLTIVTKVHDGGNHSTRPDTTDTVNTTTNNSNSTHITKKVKYHRETPELRTKALKDSVDLFKDKFEESLCVPTGSEIQRIVGRVWPYDSETPLPRRFFLFKNDSRENPLFSLLEKDISEYCLYPGQILMVEGEKTVGSNFFFSNKIYYPKQLPFYQNRKECDDVNIMVAQGPFDLYGSYADYSPLLDFCNRVGEKKPNIAIFIGPFVDDQNPNIDNLDDSYNEVFSKLLQRIAERVHPSTKVIIVPSLRDINHDPMYPQLPFSTNIKLSDNFMFVPNPCTIVINDSFTIGINNCDIISHMESKCVLKMKENQESATSTTNTTSPTAGDKDKYILQSFLNQRTYYPLYPPQVPLGISNNEILSFPSFTPDIIIMPKQTPMIKSVDNVLFTSAGNLINPNKAGGYLSMTVTNNKKESDGQMKSPVSQRTNAMIINI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZ48</accession>
    <name>A0A8J4UZ48_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Uracil-DNA glycosylase</fullName>
            <shortName evidence="4">UDG</shortName>
            <ecNumber evidence="4">3.2.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_006210</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.</text>
            <dbReference type="EC" id="3.2.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the uracil-DNA glycosylase (UDG) superfamily. UNG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.27" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000276">
        <property type="protein sequence ID" value="KAF2072478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZ48"/>
    <dbReference type="OrthoDB" id="10031947at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097510">
        <property type="term" value="P:base-excision repair, AP site formation via deaminated base removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10027">
        <property type="entry name" value="UDG-F1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00148">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002043">
        <property type="entry name" value="UDG_fam1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003588">
        <property type="entry name" value="PRK05254.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003592">
        <property type="entry name" value="PRK05254.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11264">
        <property type="entry name" value="URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11264:SF6">
        <property type="entry name" value="URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00987">
        <property type="entry name" value="UreE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3 4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uracil-DNA glycosylase-like" evidence="6">
        <location>
            <begin position="350"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="125"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="244"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="491"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="79"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="125"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="244"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="493"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="508"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="536"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2072478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="609" mass="69750" checksum="555B2AEDB88F4E1B" modified="2022-05-25" version="1">MVHDSFTYLPYYNINNINQQQQLHVQQQHQLQLQQQLQVQQQQQYQLQLQQQNYNKMQQQQQQQALNHNSMMYDLEMDKQDHQHQQEQEQKMQYHQHQQPSLQQHNNIFSTNTTKFDYFSNQNNSFNNSFNNNNNNNSSSSSSSSNSNSDSSFALGYDNKKKRKDLDNNNNNNNLFKTSPFSSSVISPFSSLNHVNNNNNNNNNNLLLFPIEYVSPPISSSTTSPLNLSPISNIYNQQQPSQFSFQHNHHQYQQQQQQQQQNQEPYQQNKRFQLSTTEYKPVVSLDQYFTNTQSFSAEIKSSLLEPGWRKVLEPEFSKDYFVKLCKFLEDEKESGGVISPKTESIFRCFNLTPFDKVKVVILGQDPPSGNQANGLAFSVDLSGTNKPIPSSLSNILTELQNDLGVCKNIKQSGVGAGNLEKWAHQGVLLLNSSLTMKKGSPDSHSSKGWEQFTDAAISLLCSQKKGIIFFLWGKLAQQKIPLIQQFNYDKDSNNSNSNNNNSNNNNSDIDKNENEKDKENIIKEKENEKKEDKLCNNSEILNNNNSNNENENNSETLISDKHIILTASHPSPFSAPKGFFGCKHFSIANNILVQSGRTPIDWDLDSLDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZ97</accession>
    <name>A0A8J4UZ97_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005983</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000256">
        <property type="protein sequence ID" value="KAF2072703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZ97"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035171366" description="Secreted protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="100" mass="11228" checksum="06CC6C9FA0F15C99" modified="2022-05-25" version="1">MKSTTLILSIIISIIFAVSFVSASPNFECSFTCTDGSKSILPFISSEENCKLAVYYSECPKRNTPEGYYCHEWCYPAINAHQAQIRLNPSHSLTFQNHAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZI6</accession>
    <name>A0A8J4UZI6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005577</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000226">
        <property type="protein sequence ID" value="KAF2073108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZI6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="334"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="481"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="256"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="381"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="392"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="486"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="520"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="545" mass="60058" checksum="F8898DD4E420C9BC" modified="2022-05-25" version="1">MGFDFILRGGQQEVASTTTSTTTTPTLSPGGGAGGGDSSPGLTMSNNKNIENNNHHHHLNPSPLYRGYVGNESTMSSSSSTPPSSPPSQSTSLAAVASSSHSFRFQPSPFNFQPQPSTSQPLPQQSLQSTFFYKTNNNIDQFQMMNSNRNNQYQPQQPPQQQNIVNGNGVPNYLNQQPYQILDHNTTANTKQSKSKNVNNAKKQKMTKQQLQQQQFQQQILLQQQQLQQHQQTQDQYNPIGYEDKIQQQLSNFYKQNKLPQSPPSSLNNSSNGNVAGPNFQKSFFNTEDTIHNNILNTINHFNNLNQSQSLQMIHQQQQQQQQLNHIKNEIQWTSKATSGSTNTSNLNGISPSSSFSDNEQQQNTTSIVDKINSYVNSKPIPKPSLSPPSSPNSNSSGGNNTTTPPSLSSNLTPKLNSLSTKDNIVSPPTDNYSLESNTTANQYINNKHNIEPVNSIDSPNSPSDDSEFLFASILMNLKEKPPLPNNRSTPNSQIFVQDPSLNKSHTSILGSKVSKKRASTSSNSSQLSKKAQKAQLAAEFQYSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0B7</accession>
    <name>A0A8J4V0B7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004464</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000158">
        <property type="protein sequence ID" value="KAF2074218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0B7"/>
    <dbReference type="OrthoDB" id="20528at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34116">
        <property type="entry name" value="PLASMINOGEN ACTIVATOR INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34116:SF2">
        <property type="entry name" value="THH1_TOM1_TOM3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="128"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="215"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="346"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="397"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="448"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="479" mass="53894" checksum="5B0FE2D8F1DDE684" modified="2022-05-25" version="1">MKTWLQVLGIIEFVVGLLVLLFLVLVSFVIVRNRHIEFNKLWRSRLFLTCSAFVWVVFLLLGNEYLWNSTDGIFNINPDHSKAVCSLHVFFTVGSSEPLFFLIFLFILKSKTSDKNQNLYINDPNYYVYKWSLLWTIPLILIHLMILILDASSVNEPKKLFWTVYDKENFKCAVPIISTASFAIFFVLFLLIFVFLSRKFSKTIINRVLINRMRLLQFTFISFLPLEVAIRVVLIFTLDIGETSIALFHAFFFVDIAVILIGILEFAFFPVIDSLGFGLFESVLKSLGERDKDYIGMNSTTTISTHSSTPNTLIPISEYEKAIKYMDTPTPGMEGADSGKNPSPAPAAAATTTNNNNRANVVVGDIEMDKLNIKHKSPILDRVRDTEPTHVPPLPLSSPLGSSPSSTLSSPRSDISHEKKKSRRFSLASIAGKSKKSPPSSPPITGMSPPQSSVKSIFQPTPVDDFKGQLFKYTQSSYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0C4</accession>
    <name>A0A8J4V0C4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Malate synthase</fullName>
            <ecNumber evidence="2 8">2.3.3.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_004458</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>glyoxylate + acetyl-CoA + H2O = (S)-malate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18181"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the malate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.9" evidence="2 8"/>
    <dbReference type="EMBL" id="AJWJ01000157">
        <property type="protein sequence ID" value="KAF2074248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0C4"/>
    <dbReference type="OrthoDB" id="186072at2759"/>
    <dbReference type="UniPathway" id="UPA00703">
        <property type="reaction ID" value="UER00720"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004474">
        <property type="term" value="F:malate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00727">
        <property type="entry name" value="malate_synt_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1220.12:FF:000001">
        <property type="entry name" value="Malate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.360:FF:000001">
        <property type="entry name" value="Malate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.360">
        <property type="entry name" value="Malate synthase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1220.12">
        <property type="entry name" value="Malate synthase, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044856">
        <property type="entry name" value="Malate_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011076">
        <property type="entry name" value="Malate_synth_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006252">
        <property type="entry name" value="Malate_synthA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019830">
        <property type="entry name" value="Malate_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001465">
        <property type="entry name" value="Malate_synthase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048355">
        <property type="entry name" value="MS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048356">
        <property type="entry name" value="MS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046363">
        <property type="entry name" value="MS_N_TIM-barrel_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01344">
        <property type="entry name" value="malate_syn_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42902">
        <property type="entry name" value="MALATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42902:SF1">
        <property type="entry name" value="MALATE SYNTHASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20659">
        <property type="entry name" value="MS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20656">
        <property type="entry name" value="MS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01274">
        <property type="entry name" value="MS_TIM-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001363">
        <property type="entry name" value="Malate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51645">
        <property type="entry name" value="Malate synthase G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00510">
        <property type="entry name" value="MALATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Malate synthase N-terminal" evidence="10">
        <location>
            <begin position="5"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Malate synthase TIM barrel" evidence="9">
        <location>
            <begin position="157"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="Malate synthase C-terminal" evidence="11">
        <location>
            <begin position="412"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001363-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF20659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2074248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="539" mass="61230" checksum="A334BC3715835E7A" modified="2022-05-25" version="1">MNLRGIEIRGVVDQHTRYILTPEVLTFLGELERRFGAIRRNLLQKRQEVQININRGITPEFSNLGTRIRESSWKCLPVPSEIQDRRVEITGPPERKMVINALNSGARVFMADFEDANCPSWFNSIHGQINMIDANKRTISFTSPDGKQYSLNKEVAVLFVRPRGWHLDEEHLYVDGLPMSGSLFDFGVYLFHNHQILKNRGSSPYFYLPKLESHLEARLWNDVFVFSQHYLGIPIGTIKATVLIETILASFEMDEILYELKDHSAGLNCGRWDYIFSFIKKFQNYPKFVLPDRAKVTMTSPFMDAYVKLLIYTCHKRGVHAMGGMAAQIPIKNNKQANDAAMEKVRLDKLREVKAGHDGTWVAHPALIPIAMEQFNLNMKTPNQIHFIPPTPNYSAVEDALLQAPAVAPGDITDEGVRSNIVVGIEYLRAWVEGNGCVPIHNLMEDAATAEISRSQIWQWIKHGAKLPNGNVIDMGYYSRVFNEEVSKLSQLHQNSSSLRTATRIFNDLIVSPQFIDFLTPACYQAVLQRENRSGGAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0S9</accession>
    <name>A0A8J4V0S9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor eIF2B subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">eIF2B GDP-GTP exchange factor subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_002500</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000069">
        <property type="protein sequence ID" value="KAF2076200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0S9"/>
    <dbReference type="OrthoDB" id="10249309at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1070:FF:000012">
        <property type="entry name" value="Translation initiation factor eIF-2B alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1070">
        <property type="entry name" value="Translation initiation factor eIF-2B, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051501">
        <property type="entry name" value="eIF2B_alpha/beta/delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042528">
        <property type="entry name" value="elF-2B_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45860">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45860:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2076200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="319" mass="35530" checksum="46AE2D5E857FF2DA" modified="2022-05-25" version="1">MTTILNKDFAWVVDRFEVMIKKESEVSVAIPAIKILTEVIRTSHATTIMGLQKDLEDATKQLKNCPFNQSISLSSVCDLFIRFVTRTSLDFPNFDQCKVNLIERGEQLSTKSSMSRTKISQLADKFIRDGVTILVHGYSRVVLALLLNAAFQGKRFSVIVTESRPDSSGYKTAIKLQAAKIPVKLIMDGGVSRIIDKVDYVLCGAEAIVENGGIVNKIGTYQISIVAKAFKKPFYVAAESFKFTRSYPLNQSDIESLKKESPNDFKVCQSCSNCEKLDLLTIDSPTLDYTPPSYITLLFTELGVLTPSAVSDELIKLYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1L6</accession>
    <name>A0A8J4V1L6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Coronin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_008125</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the WD repeat coronin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000475">
        <property type="protein sequence ID" value="KAF2070562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1L6"/>
    <dbReference type="OrthoDB" id="1850764at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000076">
        <property type="entry name" value="Coronin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015505">
        <property type="entry name" value="Coronin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015048">
        <property type="entry name" value="DUF1899"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011045">
        <property type="entry name" value="N2O_reductase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856">
        <property type="entry name" value="CORONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856:SF20">
        <property type="entry name" value="CORONIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08953">
        <property type="entry name" value="DUF1899"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16300">
        <property type="entry name" value="WD40_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01166">
        <property type="entry name" value="DUF1899"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01167">
        <property type="entry name" value="DUF1900"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50974">
        <property type="entry name" value="Nitrous oxide reductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7 8" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="DUF1899" evidence="10">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1899" evidence="10">
        <location>
            <begin position="444"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="565"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="613"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="390"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="395"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="412"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="422"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU280818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2070562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="905" mass="100774" checksum="45F39EFD13C9F128" modified="2022-05-25" version="1">MFKLNVSKYRHTSGKVDKKELWFPDVNVSNSSAASTFVKANPKYVALNWQSNTGTIGLIPLKQFGKRKGDVFVIHAPNTLNDFEFNPFNDFQIAAASDATIRLFSFKEAIASGATSYSDALVTLSGHNKTVKVWDLESGQEKYSIEFDNAILSISWNYDGSLLVATGKDCKVRIIDPRSSTVLQTGEGHQGVKPSRCVWLGNSPYVVTVGFSKMRERQIFLWDSNDLSKVLKTIPLDSSTGIINPIYDQDAQLLFISGTGDSTIRLFDLNTQFTKEPAFQELSPVPSDTPAKGICALPKRALDVMEVEIDRLLKATPNNIIPITFSMARKSKSSFAEDLFPNTNSNKPTLSSNQWLEGETRVPNLMSLNPIDNPIEDQIDELTIREEPKKSTFFNDDNSNEASSSNDNDQQQEEKQQEQEQAKSTVNSGSSTPDNITRTGIVPKIVRTSKYRHIVGTPFQKSQFYTNLKVNPNTNKSIAVNSDYIAIPWIGIGGPLAIIPHAQVGRQITVPCIEIGSALTDFTLSDFDTSVVVTGSEDSHIKVFKVPEGGLPKTGKNYFTHELDLIGHNRKILSVDFHPTAQDVLISTGGDMVVKYWDLAANGAEKLSLNGFHGDAITSVSVNINGDRLLTSSKDKKMRIFDPRSNTLVSETITHSGVKGSKSIWLGQTNNIFSVGFTKQSEREYQLWDSRNLGQPLTSGLLDQLAGVITPYYDEDTSVVYLAGKGDGTIRMFEINDSEPYVHYLTQYTSGSPQVGLAQLNKHSVDVKKCEITRFYKATDNTVEPLQMTVPRNRPEFFQDDIFVPTRTNKPTLSSEEWFDGITREPEFVSICPQGMVNLSEAPPPPKKEEKVFVQEVVDNTPSREQQITGLYNRVMKQHSNNWEELERLRKASEGDDVVSDSEWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1V9</accession>
    <name>A0A8J4V1V9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Fatty acid 2-hydroxylase</fullName>
            <ecNumber evidence="21">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">CYY_007833</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="27">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">Catalyzes stereospecific hydroxylation of free fatty acids at the C-2 position to produce (R)-2-hydroxy fatty acids, which are building blocks of sphingolipids and glycosphingolipids common in neural tissue and epidermis. Plays an essential role in the synthesis of galactosphingolipids of the myelin sheath. Responsible for the synthesis of sphingolipids and glycosphingolipids involved in the formation of epidermal lamellar bodies critical for skin permeability barrier. Participates in the synthesis of glycosphingolipids and a fraction of type II wax diesters in sebaceous gland, specifically regulating hair follicle homeostasis. Involved in the synthesis of sphingolipids of plasma membrane rafts, controlling lipid raft mobility and trafficking of raft-associated proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 23">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="21 22">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="21 22">Binds 2 Zn(2+) ions per subunit that likely form a catalytic dimetal center.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 21">Belongs to the sterol desaturase family. SCS7 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="27">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="21"/>
    <dbReference type="EMBL" id="AJWJ01000440">
        <property type="protein sequence ID" value="KAF2070847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1V9"/>
    <dbReference type="OrthoDB" id="260519at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080132">
        <property type="term" value="F:fatty acid 2-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000007">
        <property type="entry name" value="Sulfite oxidase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014430">
        <property type="entry name" value="Scs7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863:SF1">
        <property type="entry name" value="FATTY ACID 2-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863">
        <property type="entry name" value="FATTY ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005149">
        <property type="entry name" value="IPC-B_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11 21" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="20 21" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="12 21" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="8 23" id="KW-0349">Heme</keyword>
    <keyword evidence="17 21 23" id="KW-0408">Iron</keyword>
    <keyword evidence="7 21" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="18 21" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="19 21 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 21 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16 21" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 25" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0813">Transport</keyword>
    <keyword evidence="13 22" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="26">
        <location>
            <begin position="4"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="88"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="97"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="23">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="23">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRNR" id="PIRNR005149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR005149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR005149-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="KAF2070847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="353" mass="40983" checksum="291397FF1866B27E" modified="2022-05-25" version="1">MIETDSYLLSEVAKHDSAEDAWVVLDGQVFDITKFIYDHPGGGEILMEHLGKDIGQVFIDKDIHEHSVTALNMLSRYAIGTVRGYRPNAKQSTSDYIKSKKAEEKNKENKKSEPLDSSLLNLVDTSKPMVPQLKLLEGENYQKWIHSQTGLKNIIIFDNSFLELFTRWPWWYIFVLWIPVITATFTYSLMQEKSTLLYSSSIFGLGLLSWGFVEYLLHRFVFHLNTSSYWGNFFHFFIHGIHHLTPFDASRLTFPPVFSILIGIGFYKLFGSNLFTVECHKTGLPWALYGGIACGYMLYDTVHYYFHHDNLPWVPNFMKQIKTNHLNHHYKDDYTNFGVTSPIFDYIFGTYKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V225</accession>
    <name>A0A8J4V225_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">40S ribosomal protein S30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_000880</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eS30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000018">
        <property type="protein sequence ID" value="KAF2077835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V225"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006846">
        <property type="entry name" value="Ribosomal_eS30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12650">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S30/UBIQUITIN-LIKE PROTEIN FUBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12650:SF15">
        <property type="entry name" value="RIBOSOMAL PROTEIN S30, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04758">
        <property type="entry name" value="Ribosomal_S30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU364011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2077835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="66" mass="7394" checksum="C11C63B9341CB2A1" modified="2022-05-25" version="1">MGKVHGGLNRAGKVRNATPKVEKKEKKKPKVGRAKKRMLFNRRYVNVAIGFGKKKGYNTQNIPTVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2K7</accession>
    <name>A0A8J4V2K7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Chloride channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_000200</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the chloride channel (TC 2.A.49) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000004">
        <property type="protein sequence ID" value="KAF2078450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2K7"/>
    <dbReference type="OrthoDB" id="428525at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04591">
        <property type="entry name" value="CBS_pair_voltage-gated_CLC_euk_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03685">
        <property type="entry name" value="ClC_6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051280">
        <property type="entry name" value="Cl-channel/antiporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11689:SF140">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11689">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN CLC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0129">CBS domain</keyword>
    <keyword evidence="9 11" id="KW-0868">Chloride</keyword>
    <keyword evidence="6 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="280"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="315"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="357"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="403"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="483"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="506"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="546"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="13">
        <location>
            <begin position="788"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="721"/>
            <end position="747"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2078450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="835" mass="94051" checksum="A8D773107AC4A9A7" modified="2022-05-25" version="1">MSKNQVGGGHYGDDDKDVFLDTRNDSSDALISDKTALFHKQQQAAVTNNSDRGDYENYDEEDNLRKSIHITHVRGKHHFTEEDKIQMAKFESLDFPIIDNQLYREYLKKTSRANHILKTFGKWVICFFIGVLVGVAAYIVKQSVEFVNEFKFKSSEKYIEDGHKFIGFLVYYAINVLFGVLASLVIIPVGQIASGSGIPEVKGYLNGIRIPQSLNVKTLFGKLISLVFAFSSGLILGPEGPMIHIGSMLGGAIGQVKSKTLKWYPKIFWKYHNDRDRRDFISTGASAGVAAAFGAPIGGVLFGFEEASSFWSKQLTWRTFFACLIATFTTNIILQGFQMQLHDYGVLTFGFSTQYLYRYAELVGFAFIGVLGGLMGAFFVYLNARLSKWRSNFFAKKKIYYRVLEVFILITISSSVLYGASSFTKCRDTNAIIDSSTDNVELSNSTMLIHFACPENQYNDMASLSFNTLDAALRLLYSRSTNVFTVPTLIAFTIISFSLTTITSGLMLASGLFIPMMLIGASLGRLVGQVGELVFHQISPPIDPSIYAMVGSSAMMAGFSRMTISLAIIIVELTEGTQYMLPVILSVMIAKWVGDIFNESIYEHLMEQKCYPFLPTQPPKSKIKMGITDFMKTDTVTLHDVEKVSRVIQVLQSNKHHGFPVIERPQDDGTRSDNDVLDDYGNKLQYDEETTYSGLILRHQLTILLYYKIFCPKQSMPLNPRLTGGSSARKYPQRRWGRPTDYGHAPADSRMNYELMTQSLARHFPPIDKMNISKDEIENLYIDLRPYMNLSSIVANETFSFSETYQLFRTMGLRHLPVVNKKNEVVGIVTRKDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3S2</accession>
    <name>A0A8J4V3S2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008589</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000544">
        <property type="protein sequence ID" value="KAF2070094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3S2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="767" mass="91222" checksum="7E8DB4D53BB1DED4" modified="2022-05-25" version="1">MRDNKRDFLFFQVYRNTHLRNSVFDHLGRTRHGFNAFDKQGKFYYSYTDAPILDFMACKHQSLIELKLNRFKELFSSSSTDQYDESLYNHWLDIKHAHRPNPFTPEDKLRQQQEVKKMNHDYLDQYMEFIAPYCASDRFITADFIGKYGLTKKRFETIEKYHPNLHYYLEAGILRLEDGKEILDVLAREKRASFVPEGCFHDPFFEKYKDQLRLLDEWHDHEFFYEISSHADGHPEKALSYFKWLIKVSPHYNYDLYAPPSSGRPLLYSQLYGVNVVYSDKKRYEFDHFVFMCPEFLKFVIKAWPESLVDNYTIKTINDVKVAQTYLAFLQEQPNIDLPNMRISTTSSNPALLALFESVGIVDYDLKKIALGRASTPADFNLCSIKAATKDPLSYVMDLFKDPERGFLATEAYGDKYILRYAIKKKRIDFIEFYRDRIEFLDTDHLLQAYHYESMEAFEMFFANYREDFNDETLLNLTAMAIRKHDFQHTVRFFGCIEQRSESYLVDCIFWSYAFLAHADSHHRQDAIAIVRHLKSQMQQQHLDLFLEQCDSDDRRFVFLLIFIVDLDFYRLLQMPFSDLDHLSLLTKVPVSLLDHLIDRLDDQELEDLIFRLEELVVAINISPNNYGNLKTTIYLFSKFQSKIKHASILEKLCHLAMYYDNHLLVDILLHQTRVRLQIPHSHDNDYEIMTNNYQTMVQSIHATQSTYFQTHTAVANSFKDFKFKDRKQFQITMDFPVIERESTANYHPDEPLTQIPYDEVFLNYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4B3</accession>
    <name>A0A8J4V4B3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Xaa-Pro dipeptidase</fullName>
            <ecNumber evidence="10">3.4.13.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Imidodipeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Peptidase D</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Proline dipeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_008122</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Xaa-L-Pro dipeptide + H2O = an L-alpha-amino acid + L-proline</text>
            <dbReference type="Rhea" id="RHEA:76407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:195196"/>
            <dbReference type="EC" id="3.4.13.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase M24B family. Eukaryotic-type prolidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.13.9" evidence="10"/>
    <dbReference type="EMBL" id="AJWJ01000475">
        <property type="protein sequence ID" value="KAF2070559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4B3"/>
    <dbReference type="OrthoDB" id="10261878at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102009">
        <property type="term" value="F:proline dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01087">
        <property type="entry name" value="Prolidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000002">
        <property type="entry name" value="Xaa-Pro aminopeptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007865">
        <property type="entry name" value="Aminopep_P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052433">
        <property type="entry name" value="X-Pro_dipept-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48480:SF2">
        <property type="entry name" value="PEPTIDASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05195">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01011">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0224">Dipeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminopeptidase P N-terminal" evidence="16">
        <location>
            <begin position="34"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2070559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="501" mass="56264" checksum="3B8B2D877D945EB6" modified="2022-05-25" version="1">MSWNKDKTDCCETTHQDSHYSPGYYWLGNNTLKVPLVLHKENRQRLVSLFLSNHKETIQKNSYILLEGGKSVTLYDSDHEPLFKQEKYFFWAFGSDMPDCYGLISLDDALNPISTLVIPKLPAEYATWLGEIHSCEYWKSIFLVDNVIFVEDMTEFLVKNNATTLYTLSGVNADSGNSLTTPKFEGIEKFNVDTKLLYNDFSECRVYKSPKELEVLRYACDASVSAHKAVMKNIKIGMKEYESEAEFLHHSYKHWGCRNVGYTCICAANANSSVLHYGHAGEPNASTIRDGSMCLYDMGAEYHGYSSDITCSFPANGKFTEKQKIVYNAVLDASRSVIAAMKPGVNWVDMHKLAERRILSHLLTAGLLQAANGVDELIENKIGSIFFPHGLGHFLGLCTHDVGGYGGDAVPKVNSLRTTRTLKAGMFITVEPGCYFIDHLLNGAIADPVKSKYLNIPVIETYRKFGGVRIEDDVFVTENGCENYSSDLPRTVEEIESFMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5S0</accession>
    <name>A0A8J4V5S0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial substrate carrier family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_003669</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000118">
        <property type="protein sequence ID" value="KAF2075017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5S0"/>
    <dbReference type="OrthoDB" id="2161at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000004">
        <property type="entry name" value="Calcium-binding mitochondrial carrier protein Aralar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051028">
        <property type="entry name" value="Mito_Solute_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45678">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45678:SF15">
        <property type="entry name" value="MITOCHONDRIAL SUBSTRATE CARRIER FAMILY PROTEIN X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="24"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="123"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="213"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2075017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="306" mass="32825" checksum="3B2282F69A24AD79" modified="2022-05-25" version="1">MSNNNNNNIENEKQQHQVHQPVKPPFYANLIAGAIAGIIGASTVFPLDMVKTRLQNQKALPNGTTPYNGAIDCFRKIIRNEGGVRALYRGLSANLVGITPEKALKLAVNDLLRTTLQGDAPSITIGQEVMSGAGAGFCQVVATNPMEIVKIRMQISGEGGKRASLGEVVKELGIRGLYKGTGATLLRDVPFSMVYFSMYGRIKAHLTDENGHIGIGNILLSGIIAGSCAASISTPMDVIKTRVQVKPGPGDPHYNGILDCINKTMKNEGPKAFCKGLVPRIMIISPLFGITLVVYEVQKNLFASTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6K0</accession>
    <name>A0A8J4V6K0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005751</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000238">
        <property type="protein sequence ID" value="KAF2072929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6K0"/>
    <dbReference type="OrthoDB" id="20920at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36070:SF1">
        <property type="entry name" value="OS04G0165500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36070">
        <property type="entry name" value="OSJNBA0019G23.7 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="95" mass="10601" checksum="E25249AEB7A7361A" modified="2022-05-25" version="1">MRGSAGKSNKIIGFALFGVISAAFIYPYIYINKKLEKPPVEKYVAGSNLRGGFVNSGSRDMGKDNTKYNNNYAEHKRNMELKHAIADQKASENQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V883</accession>
    <name>A0A8J4V883_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Pleckstrin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_003947</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000131">
        <property type="protein sequence ID" value="KAF2074759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V883"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051092">
        <property type="entry name" value="FYVE_RhoGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673">
        <property type="entry name" value="FACIOGENITAL DYSPLASIA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673:SF263">
        <property type="entry name" value="PLECKSTRIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DH" evidence="11">
        <location>
            <begin position="436"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="10">
        <location>
            <begin position="637"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="12">
        <location>
            <begin position="793"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="85"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="363"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="35"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="105"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="121"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="180"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="249"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="260"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="321"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="378"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2074759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="886" mass="97488" checksum="567393BADB001CB9" modified="2022-05-25" version="1">MSSPYRFDRPTPPPQSSKPSNSFSSKPPAIKKETSSPQLSPTITPKSSAALNSDGSLISTPTGFGSMNIVSANSVAKQLRKQSVNSSLNLVKTPPSSSPSPSPSPLTTSTSSLSKPIVKSNSEKVDDTQTFTKKESPVLSSSTPAALPSVLHRFKSHTTSSIPSSSGNNNNNNNNYSSPSPSPSPSPSPISKPIPGGGFKKPANLSSSVGELPSIPISKRPTPTPPPKVPNSSLNSSTSSLPSFSPSSSPSPSPIPSPSLTPQFSSSPAHTTSTPPLRPQPKKPALPARGNSNGSLNSSTTPPPTTTTSTFTTPPLKPVAKSNNLGNNNNNNNNKFTINNNNSSNNNKAKKLFLKSVIKEEIEEKSSDEDEPESPRVNNNTAGTSTTSTSGASSTTTTATSSNKTNSSSEASSSSTSTSSSNNSSPKLNPKSDMVQRTYLAQELLTTEKKYINNLNRIITIFLLPLRDKAQSKDKILSTEEINGIFLNIETIFGIHKTFLQNFEDRISKWSDTQKVGDVFLEMGHYLRAYTLYSNSYNNSMLSIQSLMKSNPSFSSFLSKCLLKPASKNLNLQAYLIMPIQKNHIDYQDLKSATDFISNIAIEVDERLLQYQVAHKVLDIQNSLVGLEEDIVKPTRIFLKEGDLKKISDRVVNTRHFFLFNDYLIYSKKEKKNTFRFKYSFPLLTCWVKDIPDTQRFKNLFQIISQKKTYFLCAPTSEEKLSWMKLLNEVINKLVDQNPDCQTQRFSIYEKRGSMTPQEILNTLEKSLPSNPISSIGNSDEVDPSTKPLWLKDQMTKACMHCNSSFTMTRRRHHCRKCGKIFCNDCCPVQDFSQYIVGKKVRICKNCFEEISVQLSEMNELSNQCCDDQQQQLNSSIDNSDSQDSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8D6</accession>
    <name>A0A8J4V8D6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003757</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000122">
        <property type="protein sequence ID" value="KAF2074924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8D6"/>
    <dbReference type="OrthoDB" id="6357136at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="291"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="453" mass="51673" checksum="1F869F3D3718BBBC" modified="2022-05-25" version="1">MLSSKAFQSVVYCKSSQHMYRAFCSGGYQNAATSRTDEILSKYFPDSNNSNKINPLTTPVYSNEPMDPNQGLTDFYGTQNTFLPSGNYFNYNSHDNNKYNTKRVNRNVAGTEFLEPDFQNQNQKQKIQFKSLEEFKSDYLTNLVSDNQTAREDYENKLLNRFHSEVDIQAPYLMDRVPPLDFYKSCIESTDQSDKVYIVIENISIHTTKEDIQSMLESNDFLQVEHVELEKASDYTVAGEYLSGFAMIKLSANEMTEDFDKKIEATFKRLNLFGLNYGGIRYKTIPADCLKTIRIKNLPEDVDENKLKELLKKNYQAVAGKSIGDIDQFCKINFIQPRYKPHSPTTQKSSGNAFITFKDHLSAVNGFQLFSNINFITKRAMVDWSRSILSESSLSIQSHHARSKIKSFGEIELIKTIIDLKAQIRGLEEKILTNSTTKAATATKSSSSKRGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8J6</accession>
    <name>A0A8J4V8J6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">OTU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000844</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000017">
        <property type="protein sequence ID" value="KAF2077882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8J6"/>
    <dbReference type="OrthoDB" id="20273at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22753">
        <property type="entry name" value="OTU_ALG13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049769">
        <property type="entry name" value="OTU_OTU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050704">
        <property type="entry name" value="Peptidase_C85-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419">
        <property type="entry name" value="OTU DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419:SF93">
        <property type="entry name" value="OTU DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OTU" evidence="2">
        <location>
            <begin position="16"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="336"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="362"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="379"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="417" mass="48845" checksum="875532E493DAB37E" modified="2022-05-25" version="1">MTTLEDIDKQLRSLGLCRKEVPKDGSCLFRCVSESLFGTQNYHDRVRKQCIKYLKLNRDMFEPFACIHNPWEKYIQEMEKEGTWGGEVELQALSLIYEVNFIIYIGNSNTTRVENAFKKNILLAYCHGEHYDLVFNDRHMKHIHEFQSFVYDMVNECLGLPPSCGWVKPQFKNISLKLWESQDKVKQIRDNAIAEKLLKVQTVNKLDEYQSKQRNRNSNNNNKQKKQQTKTKDATTPPPEPQQEQQQVVEEYVDPELQEILRQIEQQELEEQRLLGLEKHFPTFNVPQQQQQQTNKVEKPQSGTVWAKQTSWTQVLEEQKNQQLLAEQALAEQKKKEEEEKLKETEEAKEDAPIQFRVSYRNNNHNNNNNRNNKRRGKNNANNNNNKGNSSNNNNNNNNNSSNNNNVGNDNNTNTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VAX5</accession>
    <name>A0A8J4VAX5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_001072</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell outer membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000024">
        <property type="protein sequence ID" value="KAF2077609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VAX5"/>
    <dbReference type="OrthoDB" id="21116at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003368">
        <property type="entry name" value="POMP_repeat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01376">
        <property type="entry name" value="POMP_repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02415">
        <property type="entry name" value="Chlam_PMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035240872" description="EGF-like domain-containing protein" evidence="11">
        <location>
            <begin position="25"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="507"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="443"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="411"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="479"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="479"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="464"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2077609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="551" mass="58441" checksum="BB52139E2B3F5653" modified="2022-05-25" version="1">MNKYWQNSIILILTILFVLNSVDATLYQCFADTTKPSTCNTNGLAIRVCPTILNCLEYLSSIKNLNSTSQFKISLPNPYYSSSTSCNLSFSFAIGLTISNVGDQTPIVDCQYRSPFIKSGSSLTVNGLHITNAINSAITAPPSQSVVSIYQTTFTNCSGVDGGALSISYPTSLTVYFSFFTNNSATGLGGAIYASTTNVFRSSFSNNTATSMGGAIYSQELTVQTSGFIGNIAQQGGGGAIFSLETHISGSTLRNNSAPQGGAIDTEFIGADSNSIYENTAQQGGAIYIRSSIRQNQMTNCKIYNNTVTSDGGALYIYSASTQSELDLTKTSIFDNKAANIGGAFYFNQTSTSFLFNGNVYNNTALNPKYADNSVAQEFGCDDSSFANCNFCSSINCNTCQKNKGICLKEQGSNEKNDEDDVGKKRGGGGSSEDDVPVLCLLSSFGCDHGTCSLKANNTFTCSCSSGYSGSKCNNGGSSSSSNSSSSEPAPTPTITPKPSKSKKDKALAISLPIVFVFLGIIFLVVVYFILKKKRPTEQGYSALPIQVESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJD3</accession>
    <name>A0A8J4PJD3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_010158</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000951">
        <property type="protein sequence ID" value="KAF2068515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJD3"/>
    <dbReference type="OrthoDB" id="190105at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="5">
        <location>
            <begin position="197"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="369"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="414"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="454"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="535"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="579"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="619"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="263"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="25"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="288"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="310"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2068515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="690" mass="78776" checksum="B7E381EA79425E86" modified="2022-05-25" version="1">MYTNNTEKKGGGREGRQGVGGGGSNNDNNNNQNDNDDNNSNNTKTTTTTTTTTTTTTTTSSINKLRFSGGIHKYDTTLPLPTNQNVYENHNTSSKYNDSEKSNWLYYGDPLVESLLKKKDLHTHDQTLLEGALISVEHELQILINKRNSLQEMIKRSIVDKEGLDQRIHDCKLSIHTKWRHVSDRYGMGPIDDEAPVSLFQLLPLEVILTIFGELRFDDIKSVSQVCKQWYRYSFSDCIWKSLFQHRWSQQDHGLATLMSRSKRRIRDNSRHTMVPQSMILPDKENDNNNSSENSDNNNNNNSQQLQLQLHHHHHHHHHHVDNHESEYSEEEEEQSSNKKLRIDWREMYKKGQKVEKNWRNGVCKTKRLLGHTDWITCLHFDGKILVTGSWDASLKVWNIDTGECKVFSSPEIPDTHREGITCVKVNGNRLVSGSTDNTLRIWDLTSGVCLHVLRGHQDGVSCLCLIDDVTIASGSLDNTIILWSLETGKLLHVFANHITGISCLYYKKNLLISGTMGGTLNVIDIPSRICLQTFHGHNDRITSIQWWDTADGVRIISSSWDYSLRVWNLQTGKTIHFLSGHTFRVRCTQVQDNILVSGSWDKTIKVWDLLTGKCIHTLFGHNLHVWSLQLEGNRLVSAGWDKEVKVWDISTGSLLYTLEGHTESIICIHFKGSKLVTAAKEIIVYDFDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJE8</accession>
    <name>A0A8J4PJE8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_010248</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000994">
        <property type="protein sequence ID" value="KAF2068427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJE8"/>
    <dbReference type="OrthoDB" id="4869960at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045717">
        <property type="term" value="P:negative regulation of fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045151">
        <property type="entry name" value="DCAF8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15574:SF40">
        <property type="entry name" value="WD AND TETRATRICOPEPTIDE REPEATS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15574">
        <property type="entry name" value="WD REPEAT DOMAIN-CONTAINING FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="302"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="332"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="84"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="170"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="387"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="93"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="170"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="187"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="259"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="396"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="414" mass="46455" checksum="53A371EED061A9C9" modified="2022-05-25" version="1" fragment="single">NNNNNNNNNNNNSPSSYSIQSILSSENSDHNYFQNNMMLHNNFLSNNSNNNNTDNFNMDINNNNTRPLPLRKSLLDIRTTVEIDVGDDDDDNYNQQGNRISNNNNNGRNNNNNNNNNQIRTSDETQSQQSDDESSNPSQSIVLDAFNPVLQDYCTSIIDHDQDNFLSTAPTSASIVQSNDIPTTERESDDSDDSDDSSSSDDSSNDDGDDQEYGEYDNDDNDDDPDTDQDSYYDYSDDDDIRDEADDQQACQDSRKSDPILPSITTSSSSSGSSNLSSIPITYKQKYDGHVSTATIKSCNFYGPNSEYVMSGSDDARLFIWEKVTGRLVRVLEGHNSCINSVIYHPYEPTIVSSGIDSSALIWEPEGNYPTPDEEIEREKKLNTFTTERKVRRQRSLQATSDSIMSRLSPCSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJR9</accession>
    <name>A0A8J4PJR9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Polymorphic outer membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009834</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000823">
        <property type="protein sequence ID" value="KAF2068847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJR9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31318">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31318:SF1">
        <property type="entry name" value="POLYMORPHIC MEMBRANE PROTEIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035288681" description="Polymorphic outer membrane protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="449"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="453"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="567" mass="59513" checksum="B15E87B04872F2A3" modified="2022-05-25" version="1" fragment="single">MRNIISKLLFIVFFTLTIVNGQNIKTYYINPTSTLTAPNCGTQQSPCQSINDAYSLVTASGLTFPTQHNFILVVNGQYNIKSTMNVYSYNIAISGPSGGGAKLSSSIQAFTVSTSAASPLSSQLSFSSVTFTGFNTGRQGILSVTSTSTVNVDFTNCLFTGNSASPLFSTPTTGIVNTRLTYTNSNVTLSTGTNPFILGKPSISMSFSNYVSNTNPLLLGYGGNVNIWNSNFASNTFTNYGVMALNATTLNITSSSFQDNSANIGGCVYVFYGSTMIFNSVFTNNRAYNFGGVLYVGSTNLVVSNSQFLSSSAAYGGAIYSSNGQSTITNSLFKDNTGTDGAAMNFAAGTHTIQDTDIYGNGGIVPVDAMDPYTYVTLSFGANIVLDSSNIISSKSNTQSNITLLYCASSVLTFKGNSVLYPQSQEMAFGCDNCNTVYAQGNSYNYTCNSDGSSSETPTSTPTETPTSTPTETPTSTPTETPTSTPTETPTSTPTETPTSTPTETPTSTPTETPTSTPTSTPTETPTSTPTETPTSTPTETPTSTPTETPTSTPTETPTSTPTETPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJW7</accession>
    <name>A0A8J4PJW7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010206</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000973">
        <property type="protein sequence ID" value="KAF2068469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJW7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="63"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="164" mass="19339" checksum="DCFE3C58F1465FCE" modified="2022-05-25" version="1">MNQQPCQKHTTLRKKGARSSTTRRWQCGSCRKYFTFPSSYDKTYLLDNYEHLFDKYHDKNSSHKSSSNTSDNSDNSSNTINHKNKLDMKSPPLPTTTRIKTPTITLNESKGISTPILEDNDESDDEYDNDYYNYDYNRYNRPLKILKYDDTDTIKKEQDDTNYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJZ1</accession>
    <name>A0A8J4PJZ1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009506</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000726">
        <property type="protein sequence ID" value="KAF2069175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJZ1"/>
    <dbReference type="OrthoDB" id="10664632at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="117"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="22"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="226" mass="26200" checksum="657E43D938154DB4" modified="2022-05-25" version="1">MNLVNKENQVNRATERVPLGNIKQSSNIVHSNNVSGKSSNFNNGGKSVEQQLRDLEEKLKSKELELKHKDQELKQKNQELKHKDQEILSLENQLNSHDLYSSNSTNSKIQNNRVRKIQQLVIDNSNLQERVKTLEENQIQYEKLSNIQNNQRIRLIHKLKNENRELLHELKQRPTLVKVVATESSTPVQQPTAEPILLNYSHSHPTRSLNFTQNYGNDVEYHPGEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKH9</accession>
    <name>A0A8J4PKH9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010538</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001179">
        <property type="protein sequence ID" value="KAF2068134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKH9"/>
    <dbReference type="OrthoDB" id="1062969at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="439" mass="48669" checksum="75B221863349C4CD" modified="2022-05-25" version="1">MVFPKKIVLNNPNEIPAHFGNVKIQGTLRDYCALDRSLQEHLPIPRVNTCFCPRTRVATNAATTQCPIRVYGGPILNMTNATAATYQSNNYKDQQALQRLVQKDVQPLFDLIQKLQDKAWNWMTADYTGTQYLAAWTNDASVKVICDGPQTIVQNGLTNTFSYEYTASWSVKSFSTNLEGNISFTFTVPRMIPQPTIALFLAQSLQTFVNRGLGFNTNSFPMALTLVASAQGYGDLNFYIPDENMGSITSNLVFAVIFIGLQDLVDPTNFRTRIQIFNWDYRNTWTINAHAFNNCLYPGIESNPGLIKTFLFPQRGSSSVSCGYLPLVYHNHQDQTIDSCQIAIQATANTIYGRDLGFSYAINTPLLGTQGQYLVGQSIDPNSFLEQAKGKYAPDLLTVAPSYNNVPVSAKMGSTVQGISSSIFNVNIQINNGTLKSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKN5</accession>
    <name>A0A8J4PKN5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000932">
        <property type="protein sequence ID" value="KAF2068555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKN5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="117" mass="13817" checksum="3E447385580417D2" modified="2022-05-25" version="1">MNLLTNDSFDCWLNFFEYIYRNQHKFQTKPSFQSIFDHIVINLNQGYYRQESEDKFEALVQRYGCVLNDSHYKYLVKHTDGLTLFGIVPDSLFVSKDYHPPPIQGSTTGRPSKKQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLD7</accession>
    <name>A0A8J4PLD7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009972</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000869">
        <property type="protein sequence ID" value="KAF2068704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLD7"/>
    <dbReference type="OrthoDB" id="16985at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013219">
        <property type="entry name" value="Ribosomal_mS33"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08293">
        <property type="entry name" value="MRP-S33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="93" mass="10538" checksum="86F075C4FE2A94F0" modified="2022-05-25" version="1">MIARTLESTYKAGLRIFGFRAEGSVNLGLKYLRKPLKGQYLVNYYPKPLLSTEIVGNTNEKAEVKAETRERDLKRGKPEVKKGAGKQALKRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLH4</accession>
    <name>A0A8J4PLH4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ABC transporter G family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_010344</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001052">
        <property type="protein sequence ID" value="KAF2068331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLH4"/>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Pleiotropic ABC efflux transporter N-terminal" evidence="4">
        <location>
            <begin position="37"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="142"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="30"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="305" mass="34371" checksum="DA3FFDE478653620" modified="2022-05-25" version="1" fragment="single">MEEYELKDFNQDSPIPESGQSSPDINISQGRHEYEDLQKELEQESQQFRLEHDTEKDIEANGPTETEEDFKLRKYFENSQRMAMENGGKPKKMGVSIRNLTVKGKGADASIIADLTTPLYWFASLFNPSSWKKSNGTTFDILHDVNAYAQDGKMLLVLGRPGAGCSTFLRVISNQRGSYVSVDGEVTYGGIPAKEWKRYRGEAIYTPEEDIHHPTLTVRETLDFALKCKTPGNRLPDEKKRTFRTKIFELLVSMFGIVKQADTIVGNEFIRGLSGGERKRLTITEAMVSSSSITCWDCSTRGLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM33</accession>
    <name>A0A8J4PM33_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009287</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000671">
        <property type="protein sequence ID" value="KAF2069395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM33"/>
    <dbReference type="OrthoDB" id="16687at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051604">
        <property type="entry name" value="Ergot_Alk_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43162">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43162:SF1">
        <property type="entry name" value="PRESTALK A DIFFERENTIATION PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="1">
        <location>
            <begin position="5"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="292" mass="32485" checksum="6C74D06B8B46D31C" modified="2022-05-25" version="1">MSLNNKILVIGGSGSSGRKVVKHLENKNQNVLVAGRDETKFENLKFSNKVSFVKFDYNNPETYKAAVENVDRVFLMAAPLDSLPEKSLEPFINFLKQLSTIKKIIFISAINAERYPIVKGENLVKGSTIPYTIIRAPFFMENLTDGFMQQELKGGSLSAPVGEHSTVWISTDDIGQVVANLFLDTNNTAYNNSTIDITGPAPVNYPEIAQLLEKYTGKTIKYNNVPPLDYSNFCLSLGMPQGSVTFLSDLFADITNDNAKTVSLKVKEITGQEPKSFESFIKDNFSKIHYNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PME5</accession>
    <name>A0A8J4PME5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">6-phosphofructokinase</fullName>
            <ecNumber evidence="4 15">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_008827</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="4 15"/>
    <dbReference type="EMBL" id="AJWJ01000585">
        <property type="protein sequence ID" value="KAF2069849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PME5"/>
    <dbReference type="OrthoDB" id="537915at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005945">
        <property type="term" value="C:6-phosphofructokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070095">
        <property type="term" value="F:fructose-6-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000002">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000008">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="entry name" value="Phosphofructokinase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009161">
        <property type="entry name" value="6-Pfructokinase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015912">
        <property type="entry name" value="Phosphofructokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02478">
        <property type="entry name" value="6PF1K_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697:SF4">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000533">
        <property type="entry name" value="ATP_PFK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00433">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="11 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 15" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphofructokinase" evidence="16">
        <location>
            <begin position="24"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofructokinase" evidence="16">
        <location>
            <begin position="409"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR000533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2069849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="790" mass="86896" checksum="6EA72CD0639691B0" modified="2022-05-25" version="1">MTSTVTTVTETITTEIVTKLEPKRMAVLTSGGDSSGMNPAIRAFARMAMIKGAQVFAIREGYNGLVNDSIVPLHWGSVGGIISRGGTIIGTARCADFRKRDGRKRAVYNLIKNKIDNLLVIGGDGSLTGANLLRSEWCGLIKDLVDDGTLDKECIEKYPTLSIAGLVGSIDNDMCGTDLTIGADTATQRILEAIDSILSTAVSHQRSFVLEVMGRNCGWLALVSGLATGADYIFVPECPPEDGWEQNMIEGLERGRVSGRRCSLVIVSEGAIDKKGNPITSSYVRQTLENAGHDARITILGHVQRGGTPTFLDRYIATRMGVDAVEYFYSTDEKDKEPALIGMDGMKVTRSPLMDCVKTTQSISTLIQEKKFNQVVDLRGSLFGEFYNIFRTCANLQRSGKSSNNHSLVILHSGGPCPGMNPAVRAFTRLGIDRGYKVYGAFNGFGGLAKDELQPLDWMTVNGWSVMGGAELGTNRSIPNDDNIQDIINVIQKYNISGVLMFGGYNGYVGISKLYKYAQKYPVLKKLSIIGAPGTIANNVPGTNISIGSDTCLNNILDALDKIKQSAVASRRLFVVEVMGAHCGYLCTMSSLASGAERSYTMEKGLTLESLTEDLEMFVDRFEKEHKIGLIIKSENASETYTTHFIYSLFKEEGKHLFDVRESILGHLQQGGTPSGIDRVFSTRLMNQYFNFLDKQLAETGEISMCGCIGFINGQIEFTPMDEMIKEMDDKYRRPHVQWWLDLDKISQDISVAPAQTNSNELKRKKEKITTPTLFSQKTFDQNVNPQWTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMS0</accession>
    <name>A0A8J4PMS0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mago nashi protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_008854</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mago nashi family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000588">
        <property type="protein sequence ID" value="KAF2069832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMS0"/>
    <dbReference type="OrthoDB" id="6495301at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
        <property type="term" value="C:exon-exon junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11295">
        <property type="entry name" value="Mago_nashi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1560.10:FF:000001">
        <property type="entry name" value="Protein mago nashi homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1560.10">
        <property type="entry name" value="Mago nashi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004023">
        <property type="entry name" value="Mago_nashi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036605">
        <property type="entry name" value="Mago_nashi_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12638:SF0">
        <property type="entry name" value="MAGO HOMOLOG, EXON JUNCTION COMPLEX SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12638">
        <property type="entry name" value="PROTEIN MAGO NASHI HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02792">
        <property type="entry name" value="Mago_nashi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89817">
        <property type="entry name" value="Mago nashi protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="22"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2069832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="21878" checksum="BA19A5158FECB175" modified="2022-05-25" version="1">MTSVNEQITDAVSTDVDISNESSGSSTATSSTTTATTKDILNSETAKEKDDFYLRYYVGHKGKFGHEFLEFEFRSGNKLRYANNSHYKNENLIRKEVCVSDNVIKELKKIIIDSGIMMEDDSLWPEPDVVGKQELEIVLKDEHISFTTTKIGSLMDVEKCEDSEGLKVMFYLIQDLKCLVFSLIGLHFKIKPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMS9</accession>
    <name>A0A8J4PMS9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">CDP-diacylglycerol--inositol 3-phosphatidyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_008670</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000557">
        <property type="protein sequence ID" value="KAF2070013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMS9"/>
    <dbReference type="OrthoDB" id="10251079at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003881">
        <property type="term" value="F:CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006661">
        <property type="term" value="P:phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362:SF4">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--INOSITOL 3-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="69"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2070013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="16686" checksum="9355B8A15BF701FA" modified="2022-05-25" version="1">MVSKIYTYVPNIIGYFRVLFAILAFMYSHDDFIKFFVFYAISALLDMADGHAARLLNQCSQFGALLDMVTDRCTTIALMVVLSHFYPAYTNVYISLIVLDIVSHMARLWTRGESHKIVKPNHLKIMKVYYGNKYFLAFMCFASY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNF6</accession>
    <name>A0A8J4PNF6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sodium/calcium exchanger membrane region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_008399</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000514">
        <property type="protein sequence ID" value="KAF2070280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNF6"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015369">
        <property type="term" value="F:calcium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004713">
        <property type="entry name" value="CaH_exchang"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503:SF74">
        <property type="entry name" value="SODIUM_CALCIUM EXCHANGER MEMBRANE REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503">
        <property type="entry name" value="VACUOLAR CALCIUM ION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="34"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="313"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="8">
        <location>
            <begin position="5"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="8">
        <location>
            <begin position="182"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2070280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="348" mass="37914" checksum="233F452D41A4756C" modified="2022-05-25" version="1">MDPLFVALISFAFLGFASWCVGEGGEILGKKYDASIIGGLIIAWLNTAPEAIFFITALSSGNTRFAVGAVSGSSIVVCTVALGCCIWIGNRNRRSGFVQLQPPVKKQCLILLVSTLIPLLLSLVEYNVFFGTFGIIFYLIFIGYSLFHKLPEDEKKEDDIELGIDGADDEEEEEHDEPLSKGVAYLFVGGALICYCSKPFIDSIVSLASSWNINPILLAFFLAPIASEMPEILESVSLSRKGNSQSINIAFSNLIGGTITKTTLLMGIFCFFGYIKEFEWESPTYSISLLLLTVCAASAASIGYFVNKLNQYHGLSLFAIFIVTGLLQYYFNVSVDSPIPPEVAEAII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNG0</accession>
    <name>A0A8J4PNG0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009318</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000678">
        <property type="protein sequence ID" value="KAF2069364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNG0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035207562" description="Secreted protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="22769" checksum="95F8A8B6FB9012EE" modified="2022-05-25" version="1">MNTTILTLLVLSAFVMGCMCENPIGATFTTGGSKSVLMFDKCHATSANTWVHVNYTSFYAQYQTQSVIDIHTYRSSDCSSQMIFFVELNGVTPFTQQYLNIDGFTKQYTVDLAWPKMSVKDSIIMNIYDKNQFDTPSSYKILPNNSELFGSTVYCNAQGVPMQKKCAHIKVNNNGPKECVESPLKGLFTQNDVTLSFTCSDLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNL8</accession>
    <name>A0A8J4PNL8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_007958</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000453">
        <property type="protein sequence ID" value="KAF2070728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057709">
        <property type="entry name" value="DUF7949"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25820">
        <property type="entry name" value="DUF7949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035294468" description="EGF-like domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="1388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1347"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="7">
        <location>
            <begin position="437"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="6">
        <location>
            <begin position="668"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="5">
        <location>
            <begin position="795"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7949" evidence="8">
        <location>
            <begin position="1053"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="4">
        <location>
            <begin position="1116"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1016"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1017"/>
            <end position="1045"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2070728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1388" mass="155626" checksum="281E2AFFA56779BF" modified="2022-05-25" version="1">MLKLFLYFLILFLSFKRSHTAPVITQVPDYFSPTDTYANYYSKECERTVSFIVSDSEPVVVTLNNGGLGIELLNNKTVGLVNHQFFRFNFTLPIDQISCQIKVVNQLNQEATLDFACQYSITPNVTEYGNRIHRVAANFNNFHIYFKINNFMVKSQDSPIVILERILTYYPSVGFNQVCYNTFYLVLFPTGNTTKSIDLDGKILIAVDPSNTKKNASLTVSEPNPYVDVSVRNWECSCQTPTSTKTFWCRFDDVKNDTANIIYSLNIANSMIFNPIYGNSNNGSYIIFYNSIVGTHPIDLRLNKFFQIFVVGAPTNVVTLSTPDLMPIELEPSSFETYAKTPYMDGGYFIQTRGLFNYYARGKQNFTNQIKFNDQKISFSPAYFLDDGIYGFANGTYDNYKRSTIIIGSKYESRKLADYYFFGSFQASSTLNPYEGDKEPPVITNFELIPIPGYNRIILLRVTITDNNSGFSRLYSSIGDNPSFILTSENLIKGTLLDGVYEVVIDNPSFYIDSFYVMDYASNTDIYGISTYGVTTINPFQRVPLSNPRNYNYHIIDFEITSAKWSHSNFDVSNATYTVDFMFSVTNPNKEYSPVFKFSQLDYSFLEFNTFYGQWNETLQLYVIPVTIPRRFLSGVIKYKIYYFKDILSSYLNAAFPSSTLIVHSDDADMFGPVIEDLVQVPSNMVTIGVGGETIGWTFKVTDRLNGFESGNLTVIGEYDQVERVYELDPNAQTQQIMININAICKSQAYVIKSIYLRDSSGFVSTNDIALMNYLDSSFSRINISCTASSDINPPVLTSYAFTPHSLDAGAPDRNVIFTITVEDHGSGINLDKLPVIYILSLQRIIKQACVFGAHDSVSATFTCTIRFPHGFAFPEFAAISIFGIMDNVGNFVGYTPKELLMISLSYEVRVSVPYFNYNLNSEIYSCSEITVNGGQLVILGKSFGTDNTSVVMIDYGDGNDYSQNSIPVFYSSTLLIINDVKPFTSFIRIRVNKNNLLSNEYNILPKPLPIYPPVPNESSESYESSVSSSVASSSSAQPSSSSEELPTQPPNPCSSNCGGPSQGVCSSTGCICNPPWIGKDCKSKTIVIPTPSINNTTPSTTIIVPSNTTDKNENLAFNGLIAIVELQELNQNGKLVYKYPFTQWIWSNISTDNEPIKYLYSTNITNQLDQSITNVSVSIQYFDKDENITFAGELLNMNQYSIKYSINITSYSFTNSLNQLQLIMKVSLESQQNQGCSALESGNTTVTNSEYVKLQVDDHSLYGRFIKRSIIDGRIASVTNNLLPNYNGESNQFNNINSYIGINVRSYRQLVQLDPDFSVLIDQRPAADNDNSVCSSKSKKLSTPQLIGIIIGSVAFAAIIGASLTYYLLKKREDSVFSNKLSKISRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPA6</accession>
    <name>A0A8J4PPA6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_007752</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000431">
        <property type="protein sequence ID" value="KAF2070928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPA6"/>
    <dbReference type="OrthoDB" id="8048523at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015184">
        <property type="term" value="F:L-cystine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005282">
        <property type="entry name" value="LC_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13131">
        <property type="entry name" value="CYSTINOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13131:SF7">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2070928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="221" mass="25151" checksum="F8A8E70EE785F5BC" modified="2022-05-25" version="1">MIDKINSIPLHQVYSALGIFSSICFGVQYLPQMYLNYQRRSVQGFSATGIIIKLIGASFLLINSWLIGESLPVVFYGLINVIQHTIFMFQFSIYDPAKATKYLPWVMFPIIPYLIGYRYPQTMYFTNSIKPITQVLSHLPQVILCYRSKSTLGVSLPSQYLNLFGGVAGVLMCIGIPPVSRITYFIYAFSVVQAISLFLIYFYYDIIQFKKNNKSSSNFGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPM2</accession>
    <name>A0A8J4PPM2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000364">
        <property type="protein sequence ID" value="KAF2071538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPM2"/>
    <dbReference type="OrthoDB" id="18049at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="161" mass="17988" checksum="40C7DEDC3EC40556" modified="2022-05-25" version="1">MSGNDKTGNIIKQSIPVDDTQFKPIYLQMPEPKDDFDRVKLSLITEFPSCFQIINSAVDCDRAQHSKIPGKPGSSKKCEKLRAHLNFCILSTFCPMESSALVHCMGGKIPFDSETPLRCSSDFQNFDNCLQKKATDFEANPNPPPPKAKVMYVDENTTLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPQ7</accession>
    <name>A0A8J4PPQ7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetratricopeptide-like helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_007061</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in endocytosis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YPP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000357">
        <property type="protein sequence ID" value="KAF2071613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPQ7"/>
    <dbReference type="OrthoDB" id="29013at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051722">
        <property type="entry name" value="Endocytosis_PI4K-reg_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23083:SF464">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT DOMAIN 7, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23083">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN, TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="660"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="729"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="546"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="579"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="554"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2071613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="775" mass="87888" checksum="63E0F3D345B12A82" modified="2022-05-25" version="1">MSMRNKKQISLEIEIEKARFEKDWASLANLLKKYAKLNNISESPLECLVQAERFISEDDYSNALIALNKAYSLDPNNQEVIAYLAVVEYKRNDLALAMKHLSKLNNPFTNINSNDVLLNNRKVGLLIQAFNIKGKCYENQGDPQSALTSYQNVTDIAFRYFKNLRLIDSNTKPFIEDSLLRVAMIQKYFGNTQIAIKQIRSSLSLQMGLTSNSFVKSLWTLSQLLLRNVPSASFIPMMDISDPNNAAVQSKQQFYIPHDEVEEAILALQNAEVVLPNTNMDIGHQSLLYEDLCLAYSRKQQFYNIVEIYEKSLVVKFNDHHKWIQLALALYSCGKYKRSLFIIEECLSYEPKSTTLLLLASKICINHLSQMTKGIAFAKEAIQCIDQEDSTLLSTAYLSIGVAYCKKAIECKSYNEKQTNQELSLFNLKKAYILDPYDYRASYQLALIYSDIRDTPLGLKYIHESLGLNSDEPSSWNLLSILLSSNKNYELAYRACKHALSQSPTNVELLLTKAKIEMALDDGSQSLITYKTVFTFLSNDTLTSCRDNDDTESLPRNSRNQSGPSSVVSFDIRSATTADKSHKSSTLQSLSETDTFEDSPQSTKDLLRATSGSTSYASNTEIARRVHLWLSLCEAFTQQRMFKDAALCLAQAESLDNNNADIFYHQGYLLEIQDYRDKAISHYHRALTIDASHTATSIRLAGYYLRENDLLLAENNLTTILRSADPTSHQAWFQLGLVLKSKGEIERSSDCFKRAIELDKTAPLIPYNTIPRYIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPS8</accession>
    <name>A0A8J4PPS8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Histidine kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_006693</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000320">
        <property type="protein sequence ID" value="KAF2071980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPS8"/>
    <dbReference type="OrthoDB" id="20520at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16922">
        <property type="entry name" value="HATPase_EvgS-ArcB-TorS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000010">
        <property type="entry name" value="Sensor histidine kinase RcsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339:SF5">
        <property type="entry name" value="HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0807">Transducer</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="6">
        <location>
            <begin position="355"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="595"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="1013"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="313"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="725"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="848"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="913"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="967"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1096"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="326"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="725"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="744"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="848"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="871"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="920"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="977"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1107"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1121"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="3">
        <location>
            <position position="650"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="3">
        <location>
            <position position="1062"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2071980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1188" mass="132433" checksum="0AB69594331222FC" modified="2022-05-25" version="1">MNREDIDNDVVSVVLVLVIILLITIYSYYKKFEYIIYKNNQTINNNNNNNSIQQSPQQPPQPQISPIDLLFRVSFDDVLILDLQYNIISISENLIKLLDSSSGSSPSSNDETTTTFKCKEKIIVDDNESDIAKIRSINNGLNSDSSETATTTTSYNGKSFIDLLPMEYKQSVYDRFTIIINSFNKNNSDNDNNNSNNTTTTTEFDNSMSIKIPDQDQVSVNQIKMKLSPLININNTQCIVISIKHLNLKLNNEINISGNSNILENNNNSMSSSTGLSNSKEEFSFKTLQSSLELIKEEENCLISPNTTTPSSIFKKSGGIGHGTANGSNGSNSDANGANSNGSGSGGVSVDILSHISHELRTPLVSIVASTQLFRATELSVAQKEYLSIIDASSHSILDLIESVLEIGKMGSGKMTLNNVDFNLCTIVEDVCSIVSPQAQAKGIQVASYIFTHCPLCFYGDSIRLRQILLNLMGNGIKFTKEGQVIVSVEPILVTDSNITLQFRIQDTGIGISKENEHKLFNKFSQIHDGTIPQEIGGSGLGLAISRDLVELMGGKIWYEPNGDNQPGSSFFFQTTFETNPKQLPCPVPNFNEINVLIVDRNSDIRAILVKYLTEWNCNPIECASYEMAISEIKTRYKSIRNRIDVVLVDIEYLQWTDFIELKKTIDFNVYGKIGMVLMSKDRTMIHGISCLGTSKLTKPIRQSHLSACLLACIPDSPILINGSTNNSSASNSSQLGNSTGSANGNGNGNLVMNGGGNNGSTKKPPINNLSMSSTCISYVPQSTTTTTTTFTTSTTKTTYPHRYIRKSSVDTLSNHQYHQYHYSSQNHQYHQNNSGDNYEYIYPYPSSPLSSSSSSSSPYYQYSGNGDYPNGNNLNHSFNNLHDNNNQFYPIRKVTRRHSIDIVIYHDKNKDYKNLIDPSSQSIQSNSNNSNNSNNSNSNNNRNNNHSSTLSPLKKHQFENILTVNEESPPLTPTYNNNNNNNNNNNNNNNNNTTSNNANNEKQTNTSNDKIHVLIVDDNQVNLKLTQKFVQMAGYPNTAVSSAQEALNSLDHGHYSIILMDCEMPHINGFQCTEFIREREMERLNFKSNQSLINDKLNHNNYNNDDDGHNDNNDDKEINSNHNNNNNNNNSIPRIVIIALTAHNSQDSIQIQKCFDSGMDDYISKPFKPNQLESILDKWDKWLLMNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPX7</accession>
    <name>A0A8J4PPX7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Saposin B-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_007987</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000458">
        <property type="protein sequence ID" value="KAF2070686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPX7"/>
    <dbReference type="OrthoDB" id="69496at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.225.10">
        <property type="entry name" value="Saposin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007856">
        <property type="entry name" value="SapB_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008138">
        <property type="entry name" value="SapB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011001">
        <property type="entry name" value="Saposin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008139">
        <property type="entry name" value="SaposinB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051428">
        <property type="entry name" value="Sphingo_Act-Surfact_Prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11480:SF27">
        <property type="entry name" value="SAPOSIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11480">
        <property type="entry name" value="SAPOSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05184">
        <property type="entry name" value="SapB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03489">
        <property type="entry name" value="SapB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00741">
        <property type="entry name" value="SapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47862">
        <property type="entry name" value="Saposin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50015">
        <property type="entry name" value="SAP_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035237936" description="Saposin B-type domain-containing protein" evidence="4">
        <location>
            <begin position="23"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="5">
        <location>
            <begin position="30"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="112"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="139"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2070686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="152" mass="17172" checksum="08B1EB63F0854D3D" modified="2022-05-25" version="1">MKVFLILVLFSLFMTSAWVIKAEEEPSAVRSTQCQICQLIAKDVIQLLKNNVSYPQIKEDLDKFCSYLPIYSDDCTQFVNNNLQNIINYIENNDDPDKVCVGLHMCTSAVQKSTEQQQEQDSESEAVATPPPTQDAKKPKAKKDKKRLVAIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPZ1</accession>
    <name>A0A8J4PPZ1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ras guanine nucleotide exchange factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_007175</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000367">
        <property type="protein sequence ID" value="KAF2071514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPZ1"/>
    <dbReference type="OrthoDB" id="10254377at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF351">
        <property type="entry name" value="RAS GUANINE NUCLEOTIDE EXCHANGE FACTOR I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="5">
        <location>
            <begin position="395"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="4">
        <location>
            <begin position="563"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="62"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="317"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="523"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="62"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="122"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="317"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="332"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="350"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2071514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="802" mass="90103" checksum="83A395458CD63C86" modified="2022-05-25" version="1">MSNPVSINNSNGINGNNEPYVSPSSKVGTSPSSSPVSSKSSFDVNRKNKTIIVKNLLNRSNSGTSFTLGSRRTYPSSPIPSFASPLHNGDLDNDLSDHEKIPDSPKANHLSNSSIINKEHSNSTSSNSSQSEGFSSSLGGNGLINNSNSSNQHVNGDSAYIDRTLWTVRSLKEHPEIQDIMERVKPISRSIALSQTYASEETLLKHDPLIGHDTMLLLILQHLQYEGLSSSKKILEEEANVKYPDYAINESRLVSILRAAIKDSDKIYSLTLDEKKDSLLQLEEHLAFLGLLKEEQIEVGEDVNIYDEPENSNIIYSEEKEDKNKNNDKDTTTAATGATGGNTSSPQLTMSPSNSASSMGSNANLLSSSSGMSKDSSLSLSSLSLSGKSGTPIEPIKSIKAASLNKLVILLTPEKNHDLEYAKTFLLTYQSFTTPEKLLQKLIQRYHVPQKQGQTEFEWRKIAVPIQLRVVNILKTWIKDYFSDFNDKLIQTVKSFCESLRHDGNNALSNRISDTLNRKIKGLADDDEHGGSEKSKSIFTTPAPEPKVPKNIWSQTLNIFDIDDEEIARQLTLMDFEIFYNIKATELLNQSWNKPKLRSRSPNVLILINRFNEISQWTASRILDFPRIKDRSRIMARFIKIAEYCMRSLNNFNTSMAILSGLNAASVHRLKFTKEEMPKHTQQVYTELQAQLSSNSAYKEYRAILSKSNPPCLPYLGVYLTDLTFFEEGNPDYIQGFINFSKRKLIYNSISNVQSFQNTKYNLQPVYQIATLLKNLPRSDEDKLYHKSMAYEPRNKERSEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ18</accession>
    <name>A0A8J4PQ18_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TBC1 domain family member 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_006752</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Non-catalytic component of the TSC-TBC complex, a multiprotein complex that acts as a negative regulator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. The TSC-TBC complex acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. In absence of nutrients, the TSC-TBC complex inhibits mTORC1, thereby preventing phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1) by the mTORC1 signaling. The TSC-TBC complex is inactivated in response to nutrients, relieving inhibition of mTORC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000325">
        <property type="protein sequence ID" value="KAF2071937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ18"/>
    <dbReference type="OrthoDB" id="18718at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032007">
        <property type="term" value="P:negative regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039842">
        <property type="entry name" value="TBC1D7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13530">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13530:SF3">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0458">Lysosome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="7">
        <location>
            <begin position="47"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="163"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="163"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="177"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="193"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="209"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2071937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="430" mass="50150" checksum="7D767AFD279361BB" modified="2022-05-25" version="1">MRKDFRIAFLNKMGFRGGAQLKTSLEAIFEHRVIDITKLKKICHLFEIPSQYRIRVWKILLDVMPPYQEAWDLVQEQRAIEYNDLRSAVSVVKKSNSFIPLETFYIQWKDTIKKYSIDSDEDVVFRAKDLAATCLALIYHTKEQYFNFQNGLYYLGDEGSDNMPSLQQPKYNPPTFSSSNNSSHGSGKYNSSAGTRSMSYDMKQQTPTSANSQYFQQKQQQQQQRQQQQQHIISSDNNNNNNTSIQKLMEYNSNNNEHSKNIPLLDLQIDESLKSIAEAFCYIFDNEIDAFWCLNNFLNISVKQYGNKDIGLIHQINILSRLLEIHDKVIFNHFKSLNIHVDQFCTLWFKSFFTCFVPPNSIDRVWDRIIGVSMDFMAYLSFSILQSKKAIILEKSTSIELLQYLINFKDINLDIVLERSIELYSSTSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ42</accession>
    <name>A0A8J4PQ42_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007907</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000447">
        <property type="protein sequence ID" value="KAF2070775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ42"/>
    <dbReference type="OrthoDB" id="22117at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="442" mass="50471" checksum="AE474DAF33825A53" modified="2022-05-25" version="1">MFSNHEKNSLFKDALKKNGGTQKGEIVATSITPHFFSAEKYNRVAYESDLIMEAIEIVLKLYRVDKEIQAYYPELDHLKDLINIGYDNLDSFEGRLNHFSRFDLIENDNGDIGAMEFNTATPGTMMFTSSLKQIFESCDFLTDQLEHKQIVKFPLDDTRYFLNYLIGLYQRIKQTISLPTIAFVQNKNYKTINTDLDQFLQVGKEMGLDVLVAGYDELVFNGKALSYNGKSIDLIWNKFTRETVNGIVRPNYYTKTPDEAKDWIQAVKDHKVISVNGFPSIFVSENKKTLALLLDKRFHHHFTNEQIKAIQSLILPTFILKSNQDNFTEVKEKIINNKDEYVIKSSTDGRGRAIFIGKSMNQDDWNSLVEQTVDGPFVIQKFIKSKQTMVVCPRSSKEVLMNTSLAIFIIEGKSVGLSARSSPSLIQNVFSGGIMQVIYIVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ95</accession>
    <name>A0A8J4PQ95_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006644</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000316">
        <property type="protein sequence ID" value="KAF2072033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ95"/>
    <dbReference type="OrthoDB" id="2367136at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.960.10">
        <property type="entry name" value="VSR Endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="541"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="551"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="592" mass="70015" checksum="9F802E40BA069B3F" modified="2022-05-25" version="1">MKGIKRSFQCNLSYLHQNRATLNMFSLPSSCKHNGFSKTLFPPTTSISICRYYFSSTIATLDHTITPKGNSSSNTTTISDSQEIELKNFISKELEINSLNDWYKKDILHLIKDKISSSGSSNDTNDNGLLLFKQFDDVLKTRYNNSIFEFLSKHYPDYQWNRWDFYQFVNHPQGFWSTNDNRLEFVNYLCEKLKFDKHKPDHDQDHQFYSQLYKLKKSDFQQYGGSRLIKVFQDSVYSFLCDLYPNYNWEPWMFERAPKKFWGDLNNQKIYINWLGNKLGFKNYNQWYGISKADFVDNNGATLLLLYGGSPVKAVAEILKDKFQWNMWLFTHLPRGMYQDKEIVRGFIEFAKQYYEVTDMDDWYRVSWTQLRDIGGFNLIKKNGGLVKCLQEHYPHHQWDAEKFTLPGKKSSQRLLKLYLQRLFPSTEIHEDYRHESLKFEALSNFPFHLDFFLPAFSLAFEYQGKQHYQDTAAFGQTLIYQTRDKEKRQICAKNNIQIIEIPYWWNNQIDSLIGTILLECPQLSNSIHIQEKEKVDIQNDHIHSSSSSSDNTDNQEIAASTIANKDKQYLPIPKEYQKLSTQKKIFYNKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQC1</accession>
    <name>A0A8J4PQC1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein uL15/eL18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_006891</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000338">
        <property type="protein sequence ID" value="KAF2071784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQC1"/>
    <dbReference type="OrthoDB" id="6353017at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.100.10.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000039">
        <property type="entry name" value="Ribosomal_eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17135">
        <property type="entry name" value="Ribosomal_L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="5">
        <location>
            <begin position="3"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="166"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2071784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="181" mass="20640" checksum="4FD33BC6CEA197A8" modified="2022-05-25" version="1">MAVDRSHRFARKTGRKTLVSQNPYLALLVKLYKFLARRTNSKFNKIVLKRLFHTKTNRPPVSLSRIANLMKDRKQGKIAVVVGTVVDDERLLDVPKLTVCALRFTESARSRIVKAGGQVLSFDQFALLRPRGNNTFLIKGVVKAREVYKHFGRAPGLPGSHTRPYVRSEGRKFERARSPRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQV3</accession>
    <name>A0A8J4PQV3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PA14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_006775</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prespore-cell-inducing factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000327">
        <property type="protein sequence ID" value="KAF2071918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQV3"/>
    <dbReference type="OrthoDB" id="17079at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011874">
        <property type="entry name" value="Fibro_Slime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011658">
        <property type="entry name" value="PA14_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051154">
        <property type="entry name" value="Prespore-cell_inducing_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001673">
        <property type="entry name" value="S_mold_repeat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02148">
        <property type="entry name" value="Fibro_Slime"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31137">
        <property type="entry name" value="PROTEIN PSIB-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31137:SF5">
        <property type="entry name" value="PROTEIN PSIQ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00526">
        <property type="entry name" value="Dicty_CTDC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07691">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00758">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035327881" description="PA14 domain-containing protein" evidence="5">
        <location>
            <begin position="26"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="PA14" evidence="6">
        <location>
            <begin position="111"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="410"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="410"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="463"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2071918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="527" mass="56432" checksum="53F8F886CD79A2A4" modified="2022-05-25" version="1" fragment="single">MASKYKQALVLVLIVFFYNIQNASSQATLDLSGVIRDTTPSRNPDFQYNVVTAQGIVLPTLGADNKPTYCCGDNSYPSIRPVVHNQTTFYGFFNDVAGVNIEIPYTVTLTQDPMNPNLYKYTNNAFFPIDGQGYDNATNFPGETVYRDAAGNPHNFHFCFEVHTEFEYRGGEIFTFTGDDDVWVFINDQLVIDLGGIHGALTASLNIDSLGLTIGQTYPFDFFYCERHTTESEIAITTTLPFVCPYYDGCGVCQGDNSSCCLPSNCDDRHELNTLLCITPTCTDNKTCTAFPATCSDNNVCTNDTCIPGAGCSFTPIVCNDYNPCTYDYCSSSFPYNGCVFVPIGGCVACENTTCQSEDLCIPLVCHPFNGSTCISNPINCDDGNPCTTDLCSEGICTYTVVPDCPRNTTTDTSTTTETTTSTSTTGTTAPATTGTTAPATTGTTAPATTTTSSTTEGGGTGTIPTTTTTTTTTTTSTTGPPAPPPCVCPPGYDCRLEYHPAAGWLILCTQRNTTTTTTSTSTTTRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR01</accession>
    <name>A0A8J4PR01_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein mL49</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_006671</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL49 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000318">
        <property type="protein sequence ID" value="KAF2072008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR01"/>
    <dbReference type="OrthoDB" id="19439at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007740">
        <property type="entry name" value="Ribosomal_mL49"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13477:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13477">
        <property type="entry name" value="MITOCHONDRIAL 39S RIBOSOMAL PROTEIN L49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05046">
        <property type="entry name" value="Img2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2072008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="167" mass="19164" checksum="E3D93A116DFE9214" modified="2022-05-25" version="1">MMKSIFTRFSTLHSTTRIKSLNNNNNYRFFSTEAQQQQTNITEVPSKRTAPINKLYTLKTISPLQFPIEPVHSNDLIAIKPAGLKHNLPFNVQRTENGKLPVYTEIVRSQRHVYTVLRKYSGDSDVMMKEIKNIFGDSVQIVQTDSNIKIRGKHTKALRVWLSGLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRK0</accession>
    <name>A0A8J4PRK0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">methenyltetrahydrofolate cyclohydrolase</fullName>
            <ecNumber evidence="2">3.5.4.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_005669</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.9" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000232">
        <property type="protein sequence ID" value="KAF2073023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRK0"/>
    <dbReference type="OrthoDB" id="5126881at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000005">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF6">
        <property type="entry name" value="METHENYL TETRAHYDROFOLATE CYCLOHYDROLASE _ NADP-DEPENDENT METHYLENE H4F DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="3" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic" evidence="8">
        <location>
            <begin position="97"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding" evidence="9">
        <location>
            <begin position="232"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="397"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2073023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="425" mass="47247" checksum="89C736CDF50AE752" modified="2022-05-25" version="1">MISRIPRSLQTFNGKFMPLFSAYTQQHHYSTSVISNITSPHATTSTSIPVPPQSTTEIEPTHTATTTLLDKNLDLLAKINETQNVINKKPKREPIKLNGTLLSNHIIGSIKKDVEEFKANSGVVPQLVVIYVGDNKQIESFIKMKKRASESVGFKFTLDKYDSSITEEQLIKQIQYHSEDPSVHGIIVQLPLPEHLENHKIIQSIDPKKDVDGLNSINLGQIFLSKKPLFIPCTPLGILELFKAYNIKAEGKNVVVLGRSNLVGRTIATLLSQKDMTNPCIMGGASVTLLHKYSTDIDQHLKQADIIISATGVGGLIKKENVKKGVILIDVGISHQIDPTKKSGYKLTGDVDPETYSRSIAYTPVPGGVGPLTVAMLLRNTLKSAILHYKIQTNRMNRQFNEKLQQKQLEMNIKKRNSKKVESKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSN3</accession>
    <name>A0A8J4PSN3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dephospho-CoA kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006910</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000340">
        <property type="protein sequence ID" value="KAF2071764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSN3"/>
    <dbReference type="OrthoDB" id="15234at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41930:SF1">
        <property type="entry name" value="DEPHOSPHO-COA KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41930">
        <property type="entry name" value="UPF0200 PROTEIN MJ1399"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13238">
        <property type="entry name" value="AAA_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="24787" checksum="2CABBA66B7C83CB6" modified="2022-05-25" version="1">MNNSTCKFIIGVCGLNASGKSTVCHFFKDQGYTVLSLSDIIRKTLSDRNIPENRDNLINMGNELRNKSGAGVLAMKTIDILESNKNYVIDSIRHPDEVKAFREMFLGANNNDNRKFYLISITSPPMARFNRIQSRNRIGDKVDYQQFMEIEKKEMNNPDPLGQQVARVMEMSDFVIDNDPMKISSIDQLYKEISTLYHKSINKANSSTSSSSGSPLNSGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSW1</accession>
    <name>A0A8J4PSW1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Succinate dehydrogenase assembly factor 3</fullName>
            <shortName evidence="6">SDH assembly factor 3</shortName>
            <shortName evidence="6">SDHAF3</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_005839</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Promotes maturation of the iron-sulfur protein subunit of the SDH catalytic dimer, protecting it from the deleterious effects of oxidants. May act together with SDHAF1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with the iron-sulfur protein subunit within the SDH catalytic dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the complex I LYR family. SDHAF3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000243">
        <property type="protein sequence ID" value="KAF2072862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSW1"/>
    <dbReference type="OrthoDB" id="278329at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006105">
        <property type="term" value="P:succinate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20270">
        <property type="entry name" value="Complex1_LYR_SDHAF3_LYRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008381">
        <property type="entry name" value="SDHAF3/Sdh7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13137">
        <property type="entry name" value="DC11 ACN9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13137:SF6">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13233">
        <property type="entry name" value="Complex1_LYR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2072862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="153" mass="18027" checksum="3A1CDD38D8AC5E0C" modified="2022-05-25" version="1">MSALKRGDFLKLYRDILRCHRILQEPMKSMGDQYVKNEWRLHKKVDSKVAKIFYEQWNQYYSYMLIQREQLLDSAMKIMNDTDDGKINQSPILGRELDNDQLKKMNKEQKEQLEKLKVETSTFFNPETGDANVDTLIDINGPTSTTDKKDKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSW7</accession>
    <name>A0A8J4PSW7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009833</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000823">
        <property type="protein sequence ID" value="KAF2068846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSW7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="100" mass="11131" checksum="9327E91BC1AC35A7" modified="2022-05-25" version="1">MGHHHHHHHHGHHHTEYVAYPGVAPMVVPPVYPVAPQVYPVAPIAPPVYPMGVAPQVYPMGVAPMLPTTTVVFETHGHHGHHHHGHHHHGHHHHGHHGHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTB1</accession>
    <name>A0A8J4PTB1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1764 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005558</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000225">
        <property type="protein sequence ID" value="KAF2073117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTB1"/>
    <dbReference type="OrthoDB" id="20835at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013885">
        <property type="entry name" value="DUF1764_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34066:SF1">
        <property type="entry name" value="DUF1764 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34066">
        <property type="entry name" value="GROWTH FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08576">
        <property type="entry name" value="DUF1764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="168" mass="19042" checksum="E2351C56660C3503" modified="2022-05-25" version="1">MNTSKQNLKKTPDPLVKKSTTNVKKPTPTIAKATTKSTLNTQVKKQASASALNKTDIQKESKTITTNTKVDDDDIDLDSLFQKKKENKIEETKKRKELKEAEEREAKKREMRAKKVTHDDFFDSRGVNIKERKTVDGFPVFTEEELGLDVWGEGGDTELCPFDCKCCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTI0</accession>
    <name>A0A8J4PTI0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Histidine kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_006435</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000296">
        <property type="protein sequence ID" value="KAF2072259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="18419at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16922">
        <property type="entry name" value="HATPase_EvgS-ArcB-TorS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000010">
        <property type="entry name" value="Sensor histidine kinase RcsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339:SF1">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0807">Transducer</keyword>
    <keyword evidence="2" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Histidine kinase" evidence="6">
        <location>
            <begin position="750"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="1275"/>
            <end position="1397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="130"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1187"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1443"/>
            <end position="1471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="134"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1191"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1242"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="1332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2072259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1471" mass="163279" checksum="985878F811932BEC" modified="2022-05-25" version="1">MSQQPQQPTSQSPMGSPCPYCLHCNNNHKNNNSNSNSSINKNINYESILKTESIIESLPLPLLYLTIIENSENGNITLGGGASRELLNQLTCTNQQLLDSLKTIPTALSNSLASSSTSLFIQQTSKSTSQTNLINSNNSSHSNSSSHSSSHSNSSNSSNNIDSTNIAINTNSTTINNNNNNNNNNNNSNTNITGQRKNSSPTISPLLVPQSNHPQPQLLDFSNTMDPFISLDTKSILDGVGNTRSSVGSNDSDKGNVANSLLECILDCFREQKVIRQNVILKNQISTQEYSSIMTVKPYLDGCLCTFDDIFNINLFERTRNTSSNSINSGIMTPMSPDVGKENAILLGLHTPTSVSSPSSSSSYHMGSNSFSETSSPMAAAAINVFNSSCSSPSSLAASLKPLTNSELQEKKKKRFNQDHYPLIAQNFEQLAFLLKGTPIILWRANTKGEILHFKKMDEVEIEGKLVEGRSDEEFMEWVYFKNASHFKKTFIDSVKKHQIFQFTYWFSTQKQNVLLYKFSGYPIVNRTPGSNNEIISWIGVSHNIFINGGAEVSLKSEMDLEALYKKFKAFYNMPIGERTSLIESDITSGESYTHFIEKKKHLISEEEKKSFLKCKETYNILFKLSLLGVMFSTLKGLILDANDTLLSIIGYTRAEMENGKIDWMLLTPPEYFEISARALQELKGKRWCQPIEKAYFHKSGKKVPVLITSAMIDGSTEQCITFVFDLSRYRQAEMVAIEAVKLKTQFITNISHELRTPCHGILGMTQLLLDGSTNLNIVQKESIDTIKRSTDHLINLINDILDFSKLESGKLGMEINSFELLPMIEEVINSQNSITNSKGIDLILIMGRDYPVPPVIYSDRKHLKKILMNLVGNAVKFTEAGYVLLEVSTELESGDQISLKFTIKDTGIGIPDDKLEQIFIPFGQIDGSDTRKYGGSGLGLSYCKDLIDLMGGTIRVENGDQELGQKGTTFWFSIKVSIASPSYLPNSVPAANQFFYPEYKPGNNDSKSVLIIESNQMLVMSIQSALLTVKCESVDAYQMASAIEIFENAKKASTPFDVIIISDQISYIKTLLSLVTNERVIIYGNDMNSSWEYHPQVFSYLLKPITYSKLLSCVLKSKKMESPRAGLKLLPINGNNNDFVLHHNNNSQHLNHYHQQIQLQLQLQQQQQQQQQQQQQHYDRILPMQSPRLLPLSPNSNSTYNSPLSSSLPTTPTSSSSSSSGGSMSGGSSSMNSPINNVIGSDSNTTTSANNSLSSTPQGCSSNNLMFDPKKKNSILVVEDNDVNIKVIVRQLDKLGYTSVVGVDGLKALEIITKNASLNEGNCGFSLILMDCQMPTMDGFICAKIIRELEDKGQRIPIIAMTANDAKDQCFECGMDDYLPKPVKMDNLHKTLKEWIKVTDSSTTSISSSNNSISNNIHYFSSSLSDSVVYNNYVKEKTLKLNSPPLLTPNRTSLKDHLQAQTQPPPQLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTQ5</accession>
    <name>A0A8J4PTQ5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004991</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000188">
        <property type="protein sequence ID" value="KAF2073703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTQ5"/>
    <dbReference type="OrthoDB" id="21570at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="125"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="310"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="414"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="125"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="137"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="248"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="310"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="453" mass="49925" checksum="C779384D9D456F85" modified="2022-05-25" version="1">MSNFITIDNIPRILKQKIAQNLLHPTKVVLSTDDAKRAEYCFELAQKIEDFEIVCDEVYGMIEIHKQNVLFANMSNMSIPRSASKDSEALFASLVSRLNSIKQAKANMEALMGQIQTNTAVITSPPLLSTANTPPQTTNKNINSTPTTPPTMSTTTNSNTTTTTTTSTTTPTTPMTTNTQPTNNNYNNNSSHTPMSTSTPTTPPINAVNSNSNNNNNITNSISTPTQTTSINTPTTPLLPTSSTPTQDMNNQSTYDGSENNQIQENSNLTNQDTSNLDNSLGTNIIDNNITNIPQEVNVNENINQQQQQQDFNDNNDSNQVNNNTEDIKMDDNTNTNIPQDVNNSNNENFNVIQEIDHNNDGNNNVNVDINQNPNMNNNGDITMVDGNVYTNNEMNNLPTQENNTTNNSAPISVEDYSNSNNNLEMNNQQMDNSIPNTNNEDPINIDFEMQGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTV2</accession>
    <name>A0A8J4PTV2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Anaphase-promoting complex subunit CDC26</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cell division cycle protein 26 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_005688</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CDC26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000233">
        <property type="protein sequence ID" value="KAF2073010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTV2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018860">
        <property type="entry name" value="APC_suCDC26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28579">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT CDC26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28579:SF1">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT CDC26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10471">
        <property type="entry name" value="ANAPC_CDC26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="24"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="69"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2073010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="79" mass="9104" checksum="395F92EFFBB1AA8A" modified="2022-05-25" version="1">MITKKPTRIEITLDDLEEYQKLKDEQTTLKKKQQNRNNNTNTPSNSSSNTTTDQSPYPLANSVKPPKQTSEKRIGYDFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU77</accession>
    <name>A0A8J4PU77_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005462</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000218">
        <property type="protein sequence ID" value="KAF2073235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU77"/>
    <dbReference type="OrthoDB" id="19690at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45663">
        <property type="entry name" value="GEO12009P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45663:SF11">
        <property type="entry name" value="GEO12009P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14561">
        <property type="entry name" value="TPR_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="112"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="414" mass="47337" checksum="A021CED61C203AA0" modified="2022-05-25" version="1">MINRLNIRNFTNLFNKSILNSNNSNSNYTRLLRVGAPQLQLSKRTYFTNTRSSLFNQYGGSSKKSEAETHGLVFGVKDHKQLNNNNDNQQQENKDQSQQQQQQVSAKPLVVDLDIDNFQQVTMSSKHPVVLNCYSESDSLSLQTTNKLIEFAEKYPGSFLLGNLNVDFNRDLAVNLKVQSLPAIFIIHKTKLLNNFQGPIDEEDLERLVLELVNLSGGLLEGKTIDQLHAEAESFIKSNEIEKALDIYTELYEKDTTQLSRTVIGFIECFTRLNEFDKVDDMIKVLKEKFPNEVTNLQVVACRKIIELKKEAIQVVEKHANESIDDIKSRLPGIVDQDQLLELKYELALLYFNQSDYASSIEQLLDILKKNKSYTYPNSKDSAKNQIIKILESLNPKDPLVIQSRKRFSGIMFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU99</accession>
    <name>A0A8J4PU99_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009046</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000624">
        <property type="protein sequence ID" value="KAF2069641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU99"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="286" mass="33809" checksum="868639E89F1A2318" modified="2022-05-25" version="1">MVAIKRLEVDKVKWILLGIGKKDKEMLFGYNTFKLYYNTLANAKTFQEKRDLIELGKFLKSEFLKSDLVIYNDITLAFILDKEFYLKIFENSLDHKFFVKGPLMTDEFGLLKYFVEVGPDLILSIDVENIILEFALFTSGETLKPVLYLFEKWQHLIKSESILDHLMHMAVKNDNHRLINILLHRTKIRLIKPVSPQHEHQILSRMKLVLKSSRYLRPTYFQKYPNFEIDEPLLDQHIMEALGNPIDRIRFTSFEIDSDTKIIQDIMGNMTDHKYIIQDSIDLNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUG2</accession>
    <name>A0A8J4PUG2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004914</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000183">
        <property type="protein sequence ID" value="KAF2073780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUG2"/>
    <dbReference type="OrthoDB" id="25675at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006984">
        <property type="entry name" value="Fcf1/UTP23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12416">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN UTP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04900">
        <property type="entry name" value="Fcf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="196"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="196"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="232"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="263"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="326"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="349"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="365" mass="41880" checksum="CC427E9A06602BF2" modified="2022-05-25" version="1">MKIKRIKNYKKIVQFYKTNYGFSEPFHILMDPDFLKKSIGMNINFREVIPKLLEAPRTFFYMTSCCLSEMRKCGTDVYPLELLNIAKKVEQIHCYHTHDDKARAGPADYSRSCFQRLLQKGEKKFMVAAQDHDTRVEIRKNAGVPLLFVLTNMVILEKPTTLSYQGMIKTHKLLTAVNNQEKELLLKKKLGDKYVPPVTKKQYQPTPTPATPTTTTSTKTAPSANIPKQSINKKAPTTTTTTTTTTKTETNNSNNDTTNKKRKLDDVEKKEQPIKKQEPKEEEKENTSIKPTQPTQPTKPAKPTKPTKQPNKKQKTTTPTTITTSDIKKQEEKQEEKETSKESEDVQKKKTRRKRSKANKKEEGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVG3</accession>
    <name>A0A8J4PVG3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ADP-ribosylation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_003023</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000089">
        <property type="protein sequence ID" value="KAF2075708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVG3"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003500">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="33"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="135"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2075708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="21644" checksum="260E963BF568E3E4" modified="2022-05-25" version="1">MSLQYIFNLVGDFINKIYSLFEGTKDAKIAMIGLDNAGKTTLLYKLKLGEVVTTIPTIGFNVETVEYKNLNFTVFDVGGQERLRVLWRHYFEGVKAIIYLIDSSDLERVNETKEELMKVLENDALRDATLLIYANKQDGPNAFTVSQLADRLDLHSIKNRKWFIQPAIATRGVGLYEGLDWLSKSLKESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVM6</accession>
    <name>A0A8J4PVM6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Adhesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_004154</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000142">
        <property type="protein sequence ID" value="KAF2074527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVM6"/>
    <dbReference type="OrthoDB" id="2018448at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31318">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31318:SF2">
        <property type="entry name" value="PECTIN LYASE-LIKE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035239338" description="Adhesin-like protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="498"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="460"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2074527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="531" mass="57796" checksum="11D7AA6A2989BCD6" modified="2022-05-25" version="1">MNKIQLFIYTFICLISLCFYVSGSPSATTITTLYVNSSSSVDSSLCGDSIDNACGTLSDAIYSFLNTTSIGQGNSILPPLVIEVLQDYIPSADESILFYGFNLTVQPYSGDGGGQVTFYGQNRGGAYLFNFPSVTNNTAYDSVLVINNITFDSFASGIVYYFTQTPVGISVNNCQMQSSLYNSYLFYLAGANRASYTNFYNTNYLIGVSDFLTVRGGSLVFSNGYVTSDSPDNCISLFNTSAYFENSIFSNNTGASYIIYATDSTLDIAGTMFMSNQGNYVVGASTSAVSIDGTYFISNTLLNYGPVFVNQGSLSITNSEFIGNLAYMGGAVYSWGSKLSVSNTLFQDNNAVSGMAIYMRNNNITITDSVLSYSVNHSKIYTNQNDMIYLYMVPNAQFRDTAISVGRHGVPSTSFSVFDCVDSVITYEKTDIRTEGHQVLSCQTCLVNNDNVYHYSCSSNTSDSSSGSSSTNSNSTSDSDSSNSSNDYLNVVLYQTSLVLLIFFISLLLISLVIIVSCCVRRERKTYLLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVQ4</accession>
    <name>A0A8J4PVQ4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_003780</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000123">
        <property type="protein sequence ID" value="KAF2074903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVQ4"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FHA" evidence="12">
        <location>
            <begin position="64"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="165"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="475"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="478"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="505"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="524"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="555"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="574"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="590"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2074903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="616" mass="69184" checksum="E7A6A81ECE3F6AE3" modified="2022-05-25" version="1">MNIDNPIPIISTQPQDEIIEEEEDNNNNSNNNRGNEEKKKVVVWGRLVSFHPDSPSLDLVDQEILFGRNRKRCQVVFTDITVSGIHCKIYREVTPKQTAVYVTDFSTNGTFIKGIPLGKDKTTLLQNGDMVSFTSAKINTVLSFIFQDLLSADQDQEDTEIKKHYIIQHILGTGNFSVVKKCIKRDTGDSYAVKIIDKKKYWNTSKNKNQTQSEISILKQIKHPNIISIIEIFDTERYLYIVLELATGGELFDHIKAKGRYSEPEAKIVFKQILEAVSYLHQLNITHRDLKPENILVDKLNNGESIIKITDFGLAKIIGEKEIATTLCGTPLYVAPEIIKNCLYHDGNGNAGYGKEVDAWSLGCILYILLSGRPPFDIEKKQNFHLQIDKGLYSFDFPVWTTVTSNAKDLISKLLNVDPTKRISAKDSLIHPWFTIEPELYRNATVVSSPIKAPYHTQHHVVQLPVDVKNRNIPLALNNSNNSSSSSTVTNNSPSGKRGMLQFESTNSPTSSSSKGKPTLQFSNNNNNHNNNINNNVCNNNNSHNNTLFSKFLQEKQSNSKQPVNNPSNVDTEPETKKRKDTNDHQPDLSKTINNSNSGNSSSSNSKSNPPKKLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW16</accession>
    <name>A0A8J4PW16_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Glucose-6-phosphate isomerase</fullName>
            <ecNumber evidence="3 8">5.3.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_003635</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11816"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="EC" id="5.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the GPI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.9" evidence="3 8"/>
    <dbReference type="EMBL" id="AJWJ01000116">
        <property type="protein sequence ID" value="KAF2075068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW16"/>
    <dbReference type="OrthoDB" id="5831190at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00181"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004347">
        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051156">
        <property type="term" value="P:glucose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05015">
        <property type="entry name" value="SIS_PGI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05016">
        <property type="entry name" value="SIS_PGI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1390.10:FF:000001">
        <property type="entry name" value="Glucose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000004">
        <property type="entry name" value="Glucose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00473">
        <property type="entry name" value="G6P_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001672">
        <property type="entry name" value="G6P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023096">
        <property type="entry name" value="G6P_Isomerase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018189">
        <property type="entry name" value="Phosphoglucose_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035476">
        <property type="entry name" value="SIS_PGI_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035482">
        <property type="entry name" value="SIS_PGI_2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001211">
        <property type="entry name" value="PRK00179.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469:SF1">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00342">
        <property type="entry name" value="PGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00662">
        <property type="entry name" value="G6PISOMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00765">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00174">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51463">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="5 8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2075068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="564" mass="63759" checksum="DBAAB082A2A3AF08" modified="2022-05-25" version="1">MLNKEQYPKSFHELVDHFNKIGSTFNIRKEFENDKDGSRFKDFSLETKISDSVGTFLLDFSKNRINHETLQLLFNLAKASNVEQMRDKMFSGEKINFTEQRAVLHTALRNRDPNAVIKVDGENVVPLVRQVLQKMRVFTDKVRSGQWKGYTGKKITDVVNIGIGGSDLGPVMVTQALKNYANDAELRVHFVSNIDGTHMAETVKYLNAETTLFIIASKTFTTQETITNAHSARKWFLSKLVTNSDEKHAVGQHFVALSTNEVEVTKFGILKENMFEFWDWVGGRYSVWSAIGLSVALYVGMDHFEALLSGAFDMDNHFLHTPLEKNIPVILALLGVWYNNFYKFETQAILPYDQYLSRFAAYFQQGDMESNGKSVQRDGSRVQTSTGPIIWGEPGTNGQHAFYQLIHQGQKIIPCDFIASVESHNPLGQHHQILLSNFFAQTEALMKGKNPAEVKAELTKEGLSGERLESLLPHKVFDGNRPTNSIMMQKLTPHSLGALVALYEHKIFVQGIIWNINSFDQWGVELGKQLAKSILPELADKKPIQSHDSSTNGLINFYKSNSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWE5</accession>
    <name>A0A8J4PWE5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_004714</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000172">
        <property type="protein sequence ID" value="KAF2073969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWE5"/>
    <dbReference type="OrthoDB" id="21184at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051140">
        <property type="entry name" value="GATA_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658:SF18">
        <property type="entry name" value="PROTEIN GAT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="6">
        <location>
            <begin position="316"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="56"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="195"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="384"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="61"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="214"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="384"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2073969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="492" mass="55780" checksum="45CFCE63655EAB35" modified="2022-05-25" version="1">MTNLTSEDYSNISSDDGSIYFNDSIDRLLGDNTTYNSNNSNNNNNSIDTQNQLESDDYYNKKSPNNFNNDQNNLNSNSNNSNNNIPNNTTNYINQSSDSIVPTFAKVENNTNNYYYQQTFSQPINIYQEHDKNQLNINYFKLNQYSSQLTTLLKQAYNNIFVCNDEIDTITTLGSYIQMEISSLKESLLKRRIAANQSQSSSSSPPSTNRMYIHQQQQQQQQQQHHESIVPSCSISSSTSSFSPNGYYSPNTSDLYSSMGYASTISSPVPSYSSPIKHSPVICKKKGFKGFKPSPTYINLTENMIKAQTKKSKKLTNNNRVCLNCKTSDTPEWRRGPQGAKTLCNACGIRYRLTQQQQNQQQIIQNVNFSSTLESCQTNDYINNNNINNNNNNNSNQNMYSNDNNNNNNNTNNNNNDNNSLVNSMMLECQPISTDNEPHHHNYQQLLSPNTNSPNGCLSKLRHQELGVGLSSPTLPLENIFTFDNNNKFEMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWL4</accession>
    <name>A0A8J4PWL4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_002393</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000066">
        <property type="protein sequence ID" value="KAF2076278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWL4"/>
    <dbReference type="OrthoDB" id="17210at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 68F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808:SF2">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 68F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="4">
        <location>
            <begin position="101"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="293"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="43"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="300"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="317"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2076278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1062" mass="118371" checksum="DAA62F11EA96F3AD" modified="2022-05-25" version="1">MDHDSDSADNNNNSNWKTNNTIRKWSPTYEVPQQQSNTPRENTSSTATTTTTTTPTPVSLDDALNEGGIVQAKQAITNLLNKFLKSRPTKDDLVQNKILKSEYKPIPLRYSLIDHLCNYIEKNALSLEGVFRISGSNHQIRLLWSTFASSDNIEFPLNIHTTAGALKLYIREQHEPLIAYEHFSTYISQLVNDTITTKDIVQLMNKVSADNIRVIKRILRTLIILARHSELNKMDANNMGIVFGPNLFKSKPDSLNIFSEAKYSNESVTFLITNYQSVFPDLEIPILGTETNTEDATNKIGGGGGSGGSAGSGAGSSSGSDATNNNNNSSKPISPSSTSNNASSSLSPSSALGLNSNSNTSGGNANPPLSPLVLSSPSSIINSKSLSKTQLQPIHPSLENSISLVEKVNNPISQYDKKDFSMIHHLSSKLAKTKHFKNEQIWDQYLVLKICGNLINAQKSIQNAMPATNTTNNNTLKKKSNSFQQQQQQVSLVPMYILVSSANIFFFDTQQYEIQHILPHNRLKEISLDVVNKGLFSILDAETHKISYFLVPKPKVIDLLVRNLEKGRAIAKVTNKGMANLQARKFPLLEANGSGNNQFGKLPESRPVFESLASSGFSELDVWEGTVDLLEVVKKFSNILVPAESKAVVEFLLPNIPQFNGIYRKSYKLDIKTSVYKIICLICEKAKLEPTKFLLRTLKGRTLFDNVSLSDYGLGTLFTAWQLRFIALDSPESTGNFVVEFWMPDSPEFKGMQKKAIKVDAYQPLKRIMKGLCDKLKIPNHHYYHLIGPEGEILGDNDILSSIGLGIKFKTCKMKLAKNKFPIGTNPELDTPMVKSLVNDIIEGAWTKIRDRHNERIKLYCKHMLDYIVDQTFVEISKAETVPKRVAMLSKNSRYEFYQTLAIKEEEELIISNTDGYRNSVIKARCIVDQEPEQSPNYPLYRPKINIGKGPITSLRDIKINSAKTGFLSELKAKSELNNVGNQNNNNSPVSMENNLRPQKTKAFSTPVINNPNTIVSLLSLKPGTSKLVEQLKMRMSIKFNASDVEDLVPKGSPIQTRRNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWS2</accession>
    <name>A0A8J4PWS2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Letm1 RBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002379</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000065">
        <property type="protein sequence ID" value="KAF2076323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWS2"/>
    <dbReference type="OrthoDB" id="21273at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033122">
        <property type="entry name" value="LETM1-like_RBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07766">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Letm1 RBD" evidence="2">
        <location>
            <begin position="69"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="301" mass="35186" checksum="8D46618DD44454DB" modified="2022-05-25" version="1">MVLAIYRSIQKFTKELNNIVSYGVTGFYSDRQYTKFVLLPRNPPNFLKEKSLIATINDVDDTITDTTRYHDREDYLTYKRDRLKFIPLAIFLTIPFSTFGLPFYFKYLPNILPRSFSTRETKVELYKKQLNRKRENCTKLINCVAGLKQSIVKLVASTGITSIHDIKYTMISNLSECHTIFPTKNNNQLNSLSELLGLSSWIPNTILVDKLFNIPSSQEIDSKLVLLEIKKESLTYEDLQDILFRKSIEFSDQQYTYQQLLDKALKYNQLVMEIKENSGDDNILFIRQYIILQTLILDLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXC3</accession>
    <name>A0A8J4PXC3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA ligase (NAD(+))</fullName>
            <ecNumber evidence="1">6.5.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_003701</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide.</text>
            <dbReference type="EC" id="6.5.1.2"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.2" evidence="1"/>
    <dbReference type="EMBL" id="AJWJ01000119">
        <property type="protein sequence ID" value="KAF2075005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXC3"/>
    <dbReference type="OrthoDB" id="19145at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003911">
        <property type="term" value="F:DNA ligase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17748">
        <property type="entry name" value="BRCT_DNA_ligase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.610">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01588">
        <property type="entry name" value="DNA_ligase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041663">
        <property type="entry name" value="DisA/LigA_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001679">
        <property type="entry name" value="DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013839">
        <property type="entry name" value="DNAligase_adenylation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013840">
        <property type="entry name" value="DNAligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004150">
        <property type="entry name" value="NAD_DNA_ligase_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00575">
        <property type="entry name" value="dnlj"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005932">
        <property type="entry name" value="PRK07956.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01653">
        <property type="entry name" value="DNA_ligase_aden"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12826">
        <property type="entry name" value="HHH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03120">
        <property type="entry name" value="OB_DNA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001604">
        <property type="entry name" value="LigA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00532">
        <property type="entry name" value="LIGANc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="NAD-dependent DNA ligase N-terminal" evidence="10">
        <location>
            <begin position="45"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2075005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="792" mass="89770" checksum="E66F4219D2D7FD71" modified="2022-05-25" version="1">MLRFLTKYSKESNGCSLKSTLLLRYYNIKNYSSTSSNNNNQLKDDEKKELATLREKINRYNDLYYNQSKSEISDYQYDQLFKRLETLENKLNSNIQSDSNDYNISTRVGSYPTSKGVKVRHHSRMLSLQNTYSLDDITSFKNKIIRFLVKESPTTDVNNLDFLLELKYDGIGINLVYQDLKLTKVLTRGDGEIGEDITENALHFIPQLQNLLKLDKEITINGNSHRDFEIRGEVVLNKSYLETINQYSNENDGHQYKNTRNIVSGFLRKILNHPDKEDKTESIDDIVKFDFISYSLLLNNNNNNNNNNNNNNNNNLHTQLRNLNILKYYGFQIDSGATLIKSLDIRDSDLDKDTVSHPIKFIDDYIRYWESKRYDYPWDLDGIVIKVNNIALQNILGETNRSPRWAFAYKFNAKTATSTVRDIVLQVGRTGRVTPVAEIDPVQVTGVTISRATLNNIPFLEKLFIDKSSTVVVERAGDVIPKITKVLDPVSKPLSEILPSVNGQLVCPCKIGSILEKKPGYVDYFCTHQQCPSQSSQQIHWFADKKAMDISGLGPSQIDEFIQQGYIEDLGDLYLLKQYKDKMLQLKGFSHTKVNNILDSIEASKSKGLESVLCGIGIPGIGRSNSKKLASSFGSLDAIISAESPMEIEERSGLGSQISESIHTFFHPIDPDEQEYIDQLVFKLKSNGVKLLSSTTNGDVVGDTNDNETLVPLTDTIGHRLRGKNIVITGKFDMGDRDLVKDFYQRYYGAKVQASLNSKTEFLVVGDKPADAKVKKAMELNIPIINGNNKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXY1</accession>
    <name>A0A8J4PXY1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ras guanine nucleotide exchange factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002797</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000081">
        <property type="protein sequence ID" value="KAF2075906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="546434at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.870.10:FF:000038">
        <property type="entry name" value="Ras guanine nucleotide exchange factor D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF364">
        <property type="entry name" value="RAS GUANINE NUCLEOTIDE EXCHANGE FACTOR D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="6">
        <location>
            <begin position="965"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="5">
        <location>
            <begin position="1195"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="4">
        <location>
            <begin position="1363"/>
            <end position="1593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="84"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="258"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="536"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="636"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="794"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="868"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1105"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="13"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="84"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="119"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="177"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="194"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="264"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="276"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="328"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="409"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="536"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="636"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="692"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="717"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="868"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="896"/>
            <end position="906"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1598" mass="177735" checksum="1980F0BCF4095334" modified="2022-05-25" version="1">MKKSKPAAAGKKNSTSKTNSTNNNSNSNNSNSTTNTPPQSTTPVSNSTPSSPIIAGTSSPPTSISSSTSSNSFLYWKEKESLSSSSLTSSPVLTSSPAVPSSPKSSSSINLSSPPLTGKDSSSPSLTGEKSLSSPVKRSLFSLGRKSGSELINSTTQENSNTTATTTTPTPVSSNSILKKTTSPVLVSTRNQNTSVPTPSTSKSSGSVPIVSSSTTTSTTATGDNTTSQTLPNRMKSDRKSLKNPLNIFSLDFWSNKNEDDESTTPLNQPSNTVVSPPLTSQKPSTPSTPIPTSTNVNNSNIASSPTKKPMPTSSSGKSAPPTPTKKPLPLSSSPTTSTTPTTTTPTPATPTKSALKSTTTLRKNSLNSNNNNNSNTTTPNTTTTTTTTTKSSPKEIRLSSESIGNVEPTPNTLSSTPPQPTSSSPPTSTTIGNSQVSGSLSFSSSSIEKDLPYEISDELNVTKLKSKMKNKSDMERLMNLELYTRVLAKDVEDLTSKLVNEISLRKEVESHTKELETRLKVSEARLVRYSVQISPDTLDNNNSNGTATPTSVPSTNNENTKQVEEKENKEINQAIKEREDSRINTLVVRIETLERKISTLEQSNMRLTEEVHTLRYENSLLVDPDAKKKALKKFEKEKEKEIQREKEREKEIIREREKAEAKEREREKQRLKEYAKQQKVIEKYEKENPSINNNNNTEITNNTNVSDTDSTGTNSEDLYSSTGTSTADKKSNLKKKDRGGSVSEVDGSPSKKNVAFHDNSGIVSNKAKSSSVLSSVTLRRNTARRMSAHLGHTNTFIDDDDDQDLDDKSSQASTASPAERSNLVKGYFNDLYDGDYETLKNQKLTRMRSKSLSKESEEDDLANGKFNLQQNNANHSPLQISISSGSLRSEPPMPTVSLSSSVDHSSTLKENEKKRHLTIGRMFSKKEAVYRENALMLSEKLGQRASKNELIFKNIIKGESIFGAILTEDTSDRLTNYLNSSIEYLLMNSDAIEGVLLVDVDEAKFRQMIESIENNIITDFTTCSAHMVGYAFIQYFVRMPTPLLSRISGPLILAAEISHQEYRISVIRSLVYSLPLQNRGILKILLHFLKKYLESIDKINGNSGNRQPILDTASDVSTDRKGGEEDHPEEEEPTQQQPQQPTIYKKVFKVFAKALLHTTVQNLEYASLAIEYLSRDIENFESLHQDITYMIKDGESIVKAASFDKMIEKLFDLSYGFKDPDYNYTLFYTYDYYTTSIQLLEKFINYYKIASTLNAKLNHELSITILSVAMFWMKIHHNHLSSDLMFLSKLKAFIDSTTIPPTHQTIFTYFKSFFATSVTPKKVLYERGNSFLGAGALPSTKKKLMMDKVIKDGKDIDIYQLGAAIIAAQMTIIDNEMLLAIPPSQFLHKCFTKYESSPQFHDMVDKFNEWAKWATSEILIKEKLPERVAALSFFIDLAKNCVELGNYHGASALFFGLNNSAISRLKLTWEKLSTKVQQDYERIEQLFDMSMNYKNYREEIKTTKAKIIPYLGLFPKDLIAIEEGNENFTSTSLINIEKFRLLYGMIKKIQSYQQPLFNLKVSDPIKLYLLKISTTFLEEKEYHPQSLKIEPRNSVSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PY60</accession>
    <name>A0A8J4PY60_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001477</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000035">
        <property type="protein sequence ID" value="KAF2077223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PY60"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="232" mass="26166" checksum="2B1200590A3722D2" modified="2022-05-25" version="1">MFNLIKNKLNTSFSGATVNTKDSEVDNCDEFVVVEDDMTKSAWVQVDLNNNKENEQQRLLSMYQVKYEPNLQQDVSVVPPSPTEQRQAQQPIENDLSIVPPSNLEMKVCQVKQQEEQEEQDEKVVVVPVVVEQVVADEVVVPQIKEEEPEWLQGKTKTNYISRGISDYSYTSTVDATKPYTVASNNASYTSASSNVKPYFTTSNTYSYTSTSNDIKPYTSVSKSPSSYSYSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PY82</accession>
    <name>A0A8J4PY82_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002816</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000082">
        <property type="protein sequence ID" value="KAF2075880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PY82"/>
    <dbReference type="OrthoDB" id="539213at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053354">
        <property type="entry name" value="MGDG_epimerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43558">
        <property type="entry name" value="REDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G10540)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43558:SF6">
        <property type="entry name" value="REDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G10540)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2075880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="791" mass="94100" checksum="12AD1FB6C91C1097" modified="2022-05-25" version="1">MDLYNKTIDTLVKPTTKISPTTTTKNSSNNNNDKDINLFRKSVLENNDTPYSIYSTDFNNHFILDDLVITYPMNEKEICNQRKVRQRYMESRQIFSSKDLSIEEKRERIKEVLNEFEYQQFEMSNYHFFGQTPTDDNAPHKPDFDCLTENYPNYSYASLDYPKKFFKSSIKKTNNRIPHLYRLRQYDRSGYTFRILQNILKNDQLFLKMYFNSVTMFNREIYKDENNIRYSADFVRISKNNQLVISKLIIPQPSFIDKYTLLYFEGKSNLAYDKKIEYSTDDIDHCIKKFKSTFLNLTGKEYNPNLFKQKNQEFNEKDNSNSDDNKIIDQVAYDNLDRYFYQQVETFPNSNNIPTLKNICYSVIRQLLLNNPMEFKNILNILPIEILEGLLVSCKYDQTPLGFYVFQTITEQCKSNNIKIDSLTQDKVIRIASNHDYWFAFSFEKSICQNIFFPQFNTNYSINVYENMDKFKRKQWPIENQILLKSEKSNYNSDESSIVDFKSFKNTFNTYTHNRLDENIFNWDNILVCGGMVTMCLTGDLESFDETDINIYFYGLSEEQIIARISQFINNLVKQKEWDQYSMVKTLKGSIVLSKHFPYRHISFHLEPYYNINHILVCCDIECCSFGYDGKNVYSLYKSMESINYRCNFISKHSWVLNGDIHYQNRLLKYYERGYSLISFDLKFFERHQKEDFEPHNNHSGLALLVAMARDPNVKQFIKSSILPIPYGKDIKKDNFESLIRDHILLPRYQLIQQEYLDIILSNISQHPPHRKYISLSSNMFPFISVSPKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYD5</accession>
    <name>A0A8J4PYD5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Leucine-rich repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_004355</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000152">
        <property type="protein sequence ID" value="KAF2074334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYD5"/>
    <dbReference type="OrthoDB" id="1517790at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001016">
        <property type="entry name" value="Leucine Rich Repeat, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050576">
        <property type="entry name" value="Cilia_flagella_integrity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973:SF9">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT SDS22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="280"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="299"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="334"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="369"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="396"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="433"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="491"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="505"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2074334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="578" mass="65454" checksum="D4464E03A12CA850" modified="2022-05-25" version="1">MNIDEKNLTNFIGGDIKTIETINLANKNIKSIKDLSACGKLKRLDLSKNAITTLDSIKKNTALEWINLGENQLSSMSDIKQLTKLKVLNISNNLLKKIEGLDKFTELKALVLNNNEISKIESMEYLTQLNTLVLSRNKITDISNIIQLKELTKISLTHNSIKMIPNISQLTKLKEIKLSHNKISYIDSLNENLNSLFILDLSHNLIKTYDDIEPITKLKSLKSLSLIGNPISTLPDYKEKIKELIPALDSLDGRPFSERSMNKIKKKKENEIKRVVLEKKKLRAANKMNDSDNSDNDEGDKKQIKKEKVIKKEYLGKKELKENTKRPSLTMDKSNGEKKSTRENNKPAPAKKEIKKPISELKKRKQIDQYDDEVGGDDAPEEESSSSRPEDMISKKQSKKLKKETPIKSKKVEKKESLVPSMGSSYPLIKSCATANTGYPLMSSTSNDSDDDYKVIGEDTTPTTTTTKKVEKKTPTTKKSSATPVANVKKPVAKKQTQQKKKQQEEEETILNYDEDEEVELDQLKKKKPAANSDYMRPNMSNKKLMEETNYKVEVVKQSKTKFNPNELVASSAAMAWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYH3</accession>
    <name>A0A8J4PYH3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002937</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000087">
        <property type="protein sequence ID" value="KAF2075755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYH3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="255" mass="28624" checksum="61FCEF175620F825" modified="2022-05-25" version="1">MGNTTSTSSRQSLPIKKKGRTRGSSFVQSSAYESVGIFNKDGVFPISRVRKNVEGPEALLKSDKNNVSKVGRLYLCKKDNGLAISTESTDHFCLVLDIGGHSDETNLRGACMHVSFTKDRDKNLVYIQKPINVEKEKCFNSVTDTGGLVYPHDNIEHIKALSMGHPEYAEMDMSADKNDLWYFYIVFQMDDWLTEFVEIHGNKMSAYCNCQSFAKFLHKKLSGVEFPCGAIFGQSTIDKLTYTAYSCIESSIMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYV4</accession>
    <name>A0A8J4PYV4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-alpha-acetyltransferase 30</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">NatC catalytic subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_002721</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. MAK3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000078">
        <property type="protein sequence ID" value="KAF2075965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYV4"/>
    <dbReference type="OrthoDB" id="249099at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031417">
        <property type="term" value="C:NatC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004596">
        <property type="term" value="F:protein-N-terminal amino-acid acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000199">
        <property type="entry name" value="N-acetyltransferase catalytic subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044542">
        <property type="entry name" value="NAA30-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45896">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45896:SF1">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="7">
        <location>
            <begin position="58"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="14"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00071796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00078622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2075965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="217" mass="24734" checksum="31543429320913B8" modified="2022-05-25" version="1">MTDIQNDSHASNESSIDTASASITTSNTNNTNSTYKLSSFEGDIVEFTSEQLNSSQGVLVGDIQYIGYQGETQIEALMNLIKKELPEPYTIFTYRYFLNKWPELCFLAYDKDQLIGVVISKKQPHGLLERGYIGMLVVDKEYRRKKIGSNLVKLTLDKMIKMDCDEIVLETIISNSKAIALYENLGFIRVKRLYRYYAMGADAIRLLLPIKDKFIPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ64</accession>
    <name>A0A8J4PZ64_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rab GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003011</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000089">
        <property type="protein sequence ID" value="KAF2075697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ64"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001447">
        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="214" mass="24477" checksum="9CF943EFA5C874A3" modified="2022-05-25" version="1">MANNNNLDNLNTNTQEEKVVVSLKCLLLGASGSGKSKALLRYAKSTSIDQSIGTSTGEIFKIKGVEYFDAKYRLCLHIWDKQRGILERFGTVPVNYYKNASAIFIFFDVTDRASFNFIKEELKGIPHTKEFVYELFIVGNKIDLENKRVISREEAIQFAQDNAAQYYEISAKENINVHQMFDQVGKSLFYKKINDPNFTIPKEPVPCRHLCTIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ84</accession>
    <name>A0A8J4PZ84_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rab GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003007</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000089">
        <property type="protein sequence ID" value="KAF2075694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ84"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001447">
        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="24027" checksum="1E6B9DC454831167" modified="2022-05-25" version="1">MAKNNTPQQDLERVMIKFLFRGGSGSGKTKTLIRYAENTFEEQDINNDGIDFKYKYIDDLDPNYRLCLQIWDKSTNSKRFTPQAYYRNASAVFVFFDVTDRQSFNSAKEELKGIPHTNEFIHEVYIVGNKIDLESKRVISREEAIEFAQDNAAQYYEISAKENINVHQMFDEVGKALFYKKIDDPNFAIIKPKPTPSAPPPKSSCIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZR9</accession>
    <name>A0A8J4PZR9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycerate kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_002704</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycerate kinase type-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000077">
        <property type="protein sequence ID" value="KAF2075997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZR9"/>
    <dbReference type="OrthoDB" id="10262596at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008887">
        <property type="term" value="F:glycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031388">
        <property type="term" value="P:organic acid phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10350">
        <property type="entry name" value="Glycerate kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1510.10">
        <property type="entry name" value="Glycerate kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018193">
        <property type="entry name" value="Glyc_kinase_flavodox-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004381">
        <property type="entry name" value="Glycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018197">
        <property type="entry name" value="Glycerate_kinase_RE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036129">
        <property type="entry name" value="Glycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00045">
        <property type="entry name" value="glycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21599">
        <property type="entry name" value="GLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21599:SF0">
        <property type="entry name" value="GLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02595">
        <property type="entry name" value="Gly_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006078">
        <property type="entry name" value="GlxK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110738">
        <property type="entry name" value="Glycerate kinase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="398" mass="43122" checksum="4D14680E6E9EEFB1" modified="2022-05-25" version="1">MKTLISFDKFKDCMSSFELGCITLDCLKKHTKNKSDDVKILRMSDGGEGLLESLTYSLGLETKVVNDIVGPLGTLIQSKYAYSKQHGYAVIEMALASGLEMVPVSQRNPFNTTTHGVGQLILDAVTSGFTKIIIGAGGSATNDAGLGALSAMGAIKVTLKQEDGSINNNNNNTTDIIYGKHLNHIQSITQTNIELFKNITFEFLTDVTNPMIGTNGAVYSFSGQKGASENDKIVLEKGMENVSSLYPRDISKYEGSGAAGGLPSGFIAYFNAKITKGIEFVCESYSLEKKLESGEIDLIITGEGCFDKTTLDGKVVSKIMSLADKYNIKCLVICGKKIIDDSYQTFNKDACKIFSLVDLFGAKESLTNTKLCIESLFESNLELKSFLNNCNISSPDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZU0</accession>
    <name>A0A8J4PZU0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002618</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000074">
        <property type="protein sequence ID" value="KAF2076054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZU0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.20">
        <property type="entry name" value="Apolipoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="369" mass="41324" checksum="759A1DAB1C7D82A2" modified="2022-05-25" version="1">MNFNPFPHLSPMLNDLIPPPPPQENAFHPTALDVFNAITYVSSVKSIPQEYRNHFISLDELKSLLVWEFKTLQNSGGIPDWYSYTFMYTIPFYNEIAQRAVAPSIEDIRQTIDNMNENINTTTNAMNQKINTAISTLNSNINNPIDTAINNLNTSLENAINSLNNNINTSMETAVNNINTSMETAVNKINSVSNNNINNSNDIAINNLNTSFDKAINSLNNNINTSMETAVNNINTVSKNNINNSINTAINNLNTSLDNAINSLNNNIKETISIAINSALVPLNNHLCSLSFNQRARAINAMGGEDLIPIRDENNIPPPFLPADQRNFYQITEDQADQIIAHYHLEDFVQNSSHRDKLKKIAKYINLHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZU4</accession>
    <name>A0A8J4PZU4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003368</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000104">
        <property type="protein sequence ID" value="KAF2075339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZU4"/>
    <dbReference type="OrthoDB" id="5425247at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2075339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="424" mass="46919" checksum="2CF2747E80FB52AD" modified="2022-05-25" version="1">MVLPQQLVFNNPADLPIKNIKIIGNIGEQINDYKKLHSSPGSNVSHCNFNSIQTQAVAYGGPKLGMSNRVKNIYQTNNFNEIKNLENQCSDDLTPIYKGSENLKSWVAFMDEDATQFVIRWDNNGSYIYSFPLSITPASSYANNTDPNAKYIALVQFGVYSASTGLCGTHTYKFGLTCAIPGVSAALMIAITVGKLMNSRFMYLVNEFAILLTQAANQMNIAAEFYTPKEAEGTLAGHIDFICNFIADQFYSTMFNFSRIQIFNWDTYSQWTINTQAIINASYPAGLEDPSKVYITYAAFNGSAFYPQCLVKPDNTVGYSLYLYYGHGTSITQVALQVGRAINPIEGCTYAFSVPPTGPVQNLSGAFIDPQVFLQNTKTTDWVRNQLTVSTTNSVPVTATIDHTQEKYPNIIININKGNPQSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZW7</accession>
    <name>A0A8J4PZW7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">V-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_001615</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000039">
        <property type="protein sequence ID" value="KAF2077106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZW7"/>
    <dbReference type="OrthoDB" id="190375at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15843">
        <property type="entry name" value="R-SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016444">
        <property type="entry name" value="Synaptobrevin/VAMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45701">
        <property type="entry name" value="SYNAPTOBREVIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005409">
        <property type="entry name" value="Synaptobrevin_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="5">
        <location>
            <begin position="8"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2077106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="96" mass="11033" checksum="0619059BF0E6D258" modified="2022-05-25" version="1">MSQQQPNKVHQTQNQVNEVTGIMHNAIGQMLDNQQKVSDLQEKSENMKQGASNFKKKTTEIKRLMWCRNFKITIIITLVVLLLLAIIIVPIVLKFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0I0</accession>
    <name>A0A8J4Q0I0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_000994</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000022">
        <property type="protein sequence ID" value="KAF2077678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="372487at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="PIN_XRN1-2-like"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341">
        <property type="entry name" value="5'-&gt;3' EXORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341:SF71">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06201">
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03159">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="296"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="424"/>
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    </feature>
    <feature type="domain" description="PITH" evidence="7">
        <location>
            <begin position="1055"/>
            <end position="1213"/>
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    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="377"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="504"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="744"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="378"/>
            <end position="412"/>
        </location>
    </feature>
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        <location>
            <begin position="507"/>
            <end position="531"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="540"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="751"/>
            <end position="760"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2077678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1213" mass="138349" checksum="C23A22041723736E" modified="2022-05-25" version="1">MGVPSFYRWLIEQYPKVLVNNEINNSNSNSNNNNDSNIQFSNLYIDMNGVIHSAIRIDENNQQRGTLIKKSELSDEMIQKNLYSRLEQIISSVKPTDLIYIGIDGTPPRAKSIEQRKRRFKSSKELVESLTKSFKQQQQKLNELNKANGNSNNITFESFLEEMKSSYFDSNIISPATEFMALVNVWMREYLLGVSERYPNVRIILSDSTVPGEGEHKIMDYIRDNCDANNNNVSHVFYGMDADLIFLGLSTHEKNFYILRDPLDLIYCSTCKGNDHTSYECTSAHAYRKLNSPKENKITLKHIPISCDESDIRNLFQSYGEISTILIEKANTKRPSYTATIEFKDPNTINEITARGGSWEINNERITVHPYYINVPGATNDDNATDQQTDSGTSSPTTSTSETTTTTTTTDTIANEQNEHVPENTVFITCLDSIVSEFDVKSFCIQFGSIEKINVFPSMRLKQKIAQVIFKTQESARAAFKSNGAHFFGASIVIKKAQERTAEEIANNSNSNSTTTTTTSTNGTSSGNSTTLIPQPPKQETPEEKAERERQKQQRLLEKQFKVETFLSMADPGTSKDTAFFYLGYAQWSIEKAFETYCKFDKAPLESPKASTTPVNFDFVYLNHFRDYFHYTITRGQSSEKIAKIDFNRCVNDFTLMCMMLGNDFLPHLPSVSIHNGYIELFLSWYKSYAIGGDEIKYLTKEINNSWEIQYENLLPLLKVLADWESTQFPDNLEKQFRKAFNRKNKPWNKPDTSAATSDSNNNNNNVHTLSEITNIDSSKTSSPLPEFKYNDSLYYESKLHLTSTLQVLDKKEEMTHSFIEGMLWVLKYYTKGCPSWTWFYPFHYAPLVKDLARHVEKLLKTGSDTSSHFSFETGAPIPPLVHLSSVLPKYSKSIIPPTFGKLMDPPSPISHYYRDDFEIDLNGEDVAWKGVVLLDFIDFALFKSTFANLLEQHPECLTEAEKKRNQFGHDITICNKVITESIAESPSRNSNSNPDPMKPLTQRDLSWPTRKIFTLSPFESPNSSKFASIPTTQPPKPVPELVFSPLQTQFIEWRKKHSLNGSLLVLESIDFSKCYILNQDKKNSPLLGRILSNEVFQDKSIFLKSLDDDQMIIHIGFDKPVKLSYIQLINSISNDSSPKIVKFYTNESIDFSNVEGCKPFFTFDFSKDPSKLQLFSEPIPLKTNLISNFTIFIESNFLNDKQPTLLEKIILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0V1</accession>
    <name>A0A8J4Q0V1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002972</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000088">
        <property type="protein sequence ID" value="KAF2075729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="TLDc_dom"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TLDC"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="1">
        <location>
            <begin position="39"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="188" mass="20783" checksum="EF04A0E646901A1D" modified="2022-05-25" version="1">MIKYPSSKIIDKNTFSLFNEWISIGPINYEEKPQPKISTIIDQSLFKTINSWIDPNKTISFDLLYQESLKAFTSDGHYSFSASSFHSACDGKGATITLIETTDGCVFGGYNSQSWNSNGKCSKGSFVFGYSTYGPSFGNGSGCDIRITSSQVTQIFPTGYTDHIGYGKSTLTPLQTFTLKEIEVFRIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0V9</accession>
    <name>A0A8J4Q0V9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamine amidotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000781</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000016">
        <property type="protein sequence ID" value="KAF2077903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="92161at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="GATase1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044992">
        <property type="entry name" value="ChyE-like"/>
    </dbReference>
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        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695:SF5">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="1">
        <location>
            <begin position="86"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="263" mass="30179" checksum="5CBE68F352C0F5A3" modified="2022-05-25" version="1">MKIAILVTDEYLENLDNKQYYGLYEEFSKVYDEQSKVVVERFNVINKEWPTQESQYEYGYVICGSRADAYSQLDWILWLKSKIVDLVEHDVKICGICFGHQIIAEALGGKVEKNHRGWELGQVDFGISPETTEIFKKIMSNSKKSFDGASSPSQEDCGEKTVLSLLSIHQDYVSVVPKGLMGFGSTDICETQGLIKESTQTPGLYTIISFQHHPEFTKDYLNILIDELKDTLPPQFLQESRDSIKDESDQLWCSRLIHCFFKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0Z9</accession>
    <name>A0A8J4Q0Z9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Alpha-1,3-glucosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_001989</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the ALG6/ALG8 glucosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="EMBL" id="AJWJ01000051">
        <property type="protein sequence ID" value="KAF2076732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0Z9"/>
    <dbReference type="OrthoDB" id="4983at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042281">
        <property type="term" value="F:dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004856">
        <property type="entry name" value="Glyco_trans_ALG6/ALG8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12413">
        <property type="entry name" value="DOLICHYL GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12413:SF1">
        <property type="entry name" value="DOLICHYL PYROPHOSPHATE MAN9GLCNAC2 ALPHA-1,3-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03155">
        <property type="entry name" value="Alg6_Alg8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="213"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="309"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="336"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="361"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="400"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="430"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="461"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2076732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="496" mass="57255" checksum="72C8960756790A2E" modified="2022-05-25" version="1">MSKVESKSKLNVICIFLTIVCVSILARYLISIHPYSGMGKPPMFGDYEAQRHWMEITTKTPIHQWYFNTTDNDLLYWGLDYPPLTAYLSWIYGKIGEVYERPSMELFTSRGYETDTSKLFMRATVIISDLVVWLPSVWFFVNTYYNDKDLGSRILAFLFISLQPGLLLIDHGHFQYNGVSLGLALFGITFILANRHLVGSAFFVLSLNYKQMSLYYAPAFFFYLFFSNLNLSSLSKIKQSFVNILLIGIVVILSFVLCWLPFLSIEQSSQVLHRLFPFARGLYEDKVANFWCSISILVNLKKLLSHDQIIRVCLITTLTTLIPIAVGIYKKPNNRTVFLYSLVCSAFSFFLFSFQVHEKTILLACMPISLLILKHPAIVWWFGVVSTYSMFPLLFKDQLVVPYLSVMAVYLIIGYQYQRSINASTVESKWTKLIYFTSFTGMLVSHVVFQFIQAPASLPDLWLLGVCCFSFLHFIVTMIYFHHQTFSFNPIKFKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q130</accession>
    <name>A0A8J4Q130_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA replication complex GINS protein PSF3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_001394</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GINS3/PSF3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000033">
        <property type="protein sequence ID" value="KAF2077270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11713">
        <property type="entry name" value="GINS_A_psf3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21693">
        <property type="entry name" value="GINS_B_Psf3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010492">
        <property type="entry name" value="GINS_Psf3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038437">
        <property type="entry name" value="GINS_Psf3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055221">
        <property type="entry name" value="PSF3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22768">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22768:SF0">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22466">
        <property type="entry name" value="PSF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160059">
        <property type="entry name" value="PriA/YqbF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication complex GINS protein PSF3 N-terminal" evidence="7">
        <location>
            <begin position="1194"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="domain" description="GINS subunit" evidence="6">
        <location>
            <begin position="1258"/>
            <end position="1352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="13"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="82"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="234"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="295"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="425"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="503"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1363"/>
            <end position="1383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="30"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="106"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="337"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="451"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="503"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="529"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2077270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1400" mass="158021" checksum="23F4ADC6A40C8F82" modified="2022-05-25" version="1">MIKPLNQEQILFKQDNLKDDNEPITNDNKSINTDNVDDNSNNNNNNNNNNSMSKSISSGKLSSSQSLSLGGKSHVKELKRLFESKKSTNSPAKPFLGFRRIKNQENDQNINNIDLNNNRSNINSNNDLHPNNDNSLTGNNSDQNYGTSSIYTCNLDNIISAADRKYYFYVNEAIRKNIPIVPSLAIRRKSIDLTHSPIKGSLYATKYQHTYSPSTRIISMTKTPKTVSAILEEQSPSPSVQQHKRQHTTDAAITDNNDSTWIKSKARDDDFINDRNISPSRLPISRSTFDLKFNRAGTTKEEPNIKFPSLSISSNDPVPPSSSSSSKTSKLKSFIRSNKDKKEEKEKDKQQPKASSTTVNDKDDQDFMQETTHHKPNKLFAWVSLRRPIKRSNTSDITTLPVKAKIDISHSGPSKPPILSTVVLDHKQKKQQQQQQQQQHSRDDSTIADHQQQQQQTQPRQLKLQEAVLSSTTPIPPSSSLLQEQQKPQKYVSLSTTILKKDLNNISPSSSPDEPRKIVSVAKAKPKQGGSSLSIGSNKNNINNNPNSLSPPLTVTPPALSAPSSPVLSPNSDGSPISSSIEQLPPLDGLVLSPSGKEKVLSPLKNNRLEAILNSSRTRSASVGHLLEPAPKFEIDEIPPLKVHCSSLEECLEVFDKPDPPHGRCYILDLGNCSLGDEGIIEISDNFPGNLEIADLSWNDITTDGIEVFCSMLRRTKSLITSLSLQGNSVSPQSIDLFLECIEQDSIEKKGYKGFGLNLKETGIENEGSDYIAQYISANKVPSIIGLNLTSSSVTDIGMNEFSNALKKAHCTLTTLILDENYLGDDGAVLLAEGLKFNRSLTHLQIKNTDIGERGTIELSNACRFNTTITLLDISLNPWQLNGEIALQSLYIFKQQQQLQHNHRQVKIVWKEDGMESNLDKDEINEIIEYFGPDKIVDILMGGIQFNPLVEKEKLFEQMTIEPKHLNKIFNNIINSTTGTDGHLNSLKLLVQLLPYHISLEKGVFITNFLSNLNSLIHLLDRKDDDKIDFLIIDLLDFFTLIIKSNNQSCFSKFLEFHLFNKSLDLMFEYPKINILHQSILNLFLSTISTPFISDYLEDVFDFDYLERIVDHLSVHYTLQPGKRFANFGHLLKLVKVLSDTCPNHTFFKIPFWQDFKQDILDKEMHVQDSFLCGFIPDKNQKLDQALLKMNYLDIDDILSEEKPITCKFLFDGYHLGMLDPGSATQDMIVGTKIDLPFWMASLLAKKQIISVEFPLEYQEKFQAQLRNEPDVVMMKKFIYYEKIGATLSSFFNDRALSFLLFKSFRERFLRIYNQSLHLRDSDITKIQINLTDSEKSIFQVGYKISMQYEDWKNRRAEVLLTSNSSSPSLSSSSTTTTSNNNTKDTILKKRKRFIADDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q165</accession>
    <name>A0A8J4Q165_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006070</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000264">
        <property type="protein sequence ID" value="KAF2072611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q165"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2072611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="149" mass="16990" checksum="2FEC05BB36A5807B" modified="2022-05-25" version="1">MKTGNYPNYPTEFAMFDGDENVGSASDNFHYESRDEIDAMFDKDNKFRSHSGSLRLNGDLYYINTIFTDGRNRFIKSLSMKEREDGSMSAIVSYKYFYKIDGANNVVHLVGYYYKSPPDFERVKQIYRSAAITIAGIGKNVGGSYCNKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1D7</accession>
    <name>A0A8J4Q1D7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000659</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000013">
        <property type="protein sequence ID" value="KAF2078021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1D7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2078021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="198" mass="23008" checksum="544F68CD6573F78A" modified="2022-05-25" version="1">MNKITSGPNQYDKMKAYFEKCRMLNAYQSLKRNEYVEKFKERSPYLTMILDSVLYKRLHSSIKHSYDGKFEGMGRTDTILLEGILFDVNGNPIDFNFRMRTIIYGGHSDGYSIKVYFILGKTKYAPSDTKYNEDKDVEFDAFIQSCQKELCCGDTSKLQFYEVFLLLSAIFPYKCLNNEAESGPFVTITDENHINNTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1H4</accession>
    <name>A0A8J4Q1H4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_000632</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000012">
        <property type="protein sequence ID" value="KAF2078081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1H4"/>
    <dbReference type="OrthoDB" id="266020at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002343">
        <property type="entry name" value="Hud_Sxl_RNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050441">
        <property type="entry name" value="RBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48034">
        <property type="entry name" value="TRANSFORMER-2 SEX-DETERMINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00961">
        <property type="entry name" value="HUDSXLRNA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="20"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="124"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="423"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="204"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="255"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2078081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="527" mass="58583" checksum="3FC85237319C17C9" modified="2022-05-25" version="1">MDNNNNNSNQFDPVDQDALTNLIVNNIPKTLDSDGLKVLFEKHGPLASYKVVYNRKNTSPPTTNTSTTTGAAGQQLNMGYGFVKFVNPDHASIAISSMNGYEIEPMKPIKVSYAQPTSAQSSHANLYINRLEPHITKEILKELFVPFGDVSESRILVDPTTGNSRCVGFVHFTSRRDALKAVTAMNGAIIKQQTTPIYVKFADQKEESNTSNRSKPNHKGFNNSYNQNNHQNNNNNNSNNNNNNNFRHHHHHHNNNSNNIQNTNSNININTNINNNHQNNQTNQPNQTNTQTSNNNYTQNSLINQNNSNNYFNTNQGYNHYFNNSNNLTNIEGGGFNFNSTTNNGGGASNFMNNGFYDHSLNTISPPTTRYDQMGLSSYHQQGTYLQNNFPTSSASSFYSPQQIDPSMDMYNQGYIGNMFSMFSVHISNLAVEIEENYLYKLFGPFGALSGIQLIGNGQAIVNFMDYDSAQNAINNLNGKIFSGRPIQVVFLNSAQSTTSPEISANQIPSYHYPSYNNNGNGSNGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1I9</accession>
    <name>A0A8J4Q1I9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001598</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000039">
        <property type="protein sequence ID" value="KAF2077089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1I9"/>
    <dbReference type="OrthoDB" id="29321at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="613" mass="67543" checksum="E8B6E291C34DFA68" modified="2022-05-25" version="1">MDSNQLFFTAFRDGYLRSLITDQVIGGGLISITLSYLNINHKHLYLLDRDTTLVRLNINQLSQLDEYLDHPHRELINSLYIEIEDPSKIQARVFPSSVREIKLCCTKDESESQGELDVAFIPSSVSKIDLFNVHGSIKGCFSDSVTEITIGSTYTKQSDLVDSILANLPSKLEALTLFENYPIQGTCPLPETCTRLFYTGSVILLSKFQPSGKEFQHFGFGMVQTYEEFSILSGNSWIKDIGIKSLNVPLSVSCIPSSIRSIDFQDEYNLPLVPGIFPESLQDLVLETDQPLGPGVLPMKLSQLVLSTIMPIAMNVLPPNLTILSLNGFNEPLSPNVLPSSLVDLHCNSYKHPLEVGVLPSGLKTLGLSRYDSPLSPNVLPCSLKELNLFSFQQSLGPNIIPSSTTKLTLNSFNQPLVKDALPKSLIELNLPNFNKPLDSSDVLPSTLEILRLDSYSKPFPTPVSALESLKELNIASLNKSTLALIPGNIKNLSLGYKTCDDGLSLPSTLTVLNLVSRGIKTKIGKGFIPSSVKSLRMFSSNIQEGSIPEGVGYLKMQDPKLEPGYLPQSLNYLEVYINSGTIKAQDFIVKYKDILPSSLSLEIEGILYCNNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q236</accession>
    <name>A0A8J4Q236_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_001317</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000030">
        <property type="protein sequence ID" value="KAF2077389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q236"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16667">
        <property type="entry name" value="RING-H2_RNF126-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000127">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF181"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039525">
        <property type="entry name" value="RNF126-like_zinc-ribbon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PRAJA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710:SF243">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PRAJA-2 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14369">
        <property type="entry name" value="Zn_ribbon_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="244"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="299"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="302"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="365"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2077389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="391" mass="43580" checksum="8E15497F1EE217D4" modified="2022-05-25" version="1">MSSNNTTSSSTSSSSTTTSTTATTPSNSGPLYWCHICSKHVHLTNPDEVVCPDCGSEFIEEIEQPEPQPQPQPQPQPARNYPQFLQFPNTMNNPFIQRRGGHNNNLNDPFQALARIFNPSRVNVNNNNANNNNNNNFMNPFGGFMETDTSNMGASDFDQIFLNISRILGGSGGGNIVMLNGPHGGGGYIGNPGDYFQGGDWQEYLNRLFNESQKRGTPPASKEEINKLKRGVADQSIVDQKIDCSVCQEHFEKGQEYIELPCTHIYHPDCILPWLEQHNSCPVCRYELKTDDKTYEKYKNYSNNRNNQNNNNTSPSSSSSSSTSNPNPNPNNPNPTTTTTTTTTTTSTSQNNNDDLEYPEYMANGDEENVDYEIDDSDDEDTNTISFTYYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q288</accession>
    <name>A0A8J4Q288_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002029</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000053">
        <property type="protein sequence ID" value="KAF2076659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q288"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1183" mass="136300" checksum="D1CCEC0B9E147E35" modified="2022-05-25" version="1">MNKRLKWKPPPIDKEGEFEYISWFKEGELYPSNTIPKFVNRLNIFINNNENNTPDIKSLPIIPNHIECLGLYFDNSCDDINTEVGTDKCGGHTHRTIEYQDNSLYFNQNDISNDDGDEFDYEENELYPTDGYFCLEKGYIKSNITKLYIYLSQPLNLSMVLGDYIPSSVTFLSLDCLNSFSSPFIPRSVFHLEIRNQQRPFHNRLNHKAPFLPSWVTCLDISFSKETLNLGQIPSTVKRITVSDFKSPIKRKHGQIIHYRVGKEFPEFIKQEALMDNIYLKNDSRIIPRDREIKHLFISNNSSISIGEVPCGVQTIQCNSDQRFLKIGSSFPDSVTYIELTKFNQCLSNLILPSSLKCLSLDEFDQPLYGNNHLPVSTTHLVISLSYKGLDIFTNSWIFSKGYFPKSIKFLKIKTNIPKGINLPSPILVLSTIDFIDIQDEYHDQVMVVQEKEYYLNQKKFNSVGDKKITHNLKYLDLGNSFNHPISPDLLPSSLTTFNFQPHQVTFIPTVFSSKNLIPPISFDQFNFNDNNYDQLLNYKFNNIYNISNEINKKSTYKEAKDFSFINSSSNQLFFIIWRIGYLKKKIITFMDDFVPSIITLEDAIYRPRVKQFKNLYIKDAFSNNFKTPYFTNHTVKGVKVLDDKTQLQNIEYPKSVASLNIPYSSGIPHWVTQLSVSSLASKTIEIPASVTDLCITKCSPLDFKDQIPPTVKILRMASLSKDSPLIIPSSVEILYIENQYLKLDQIPKYFIPQSIKKLIFHKNVYHNVKNIPKFISKRLYGNYEIYDDSRPISSDTNFLIWNENKVITKEIALKFPPCLTTIIFGNQFNQQLLNIPDMIQDIRLGACFNQKLYSVYFPKNLKYLLIGIGFKHPILKGSLPNGLTHLRFESLDPITSKTLNNIPNTVVHLQLPKSIYSSYIRNYFEIPGSVKHLYTGFHKTIIPSTLNSVSCSEINFDTDKDQAQLQPTELELVIDPFINYRNDQEFSLKVDKNFNQFILPNSLPSIGIKRIEFGDSFNQCLVENSLPSCIESIVFGKDFNQILDQSSLPSSLTNISFGRSFNKVFSPDIFIKNPSLNRLCFKVYFEGLFDLIPNSVKELEIGENYKDVNYSFNQLMIPIERVPTQIQTLILNDSEIIYSPSRIPPTIKTLRLCKCKTYDTLPSSIESLSLGIFKKNLQEILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q320</accession>
    <name>A0A8J4Q320_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_000363</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000006">
        <property type="protein sequence ID" value="KAF2078380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q320"/>
    <dbReference type="OrthoDB" id="340962at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20524">
        <property type="entry name" value="CYCLIN_CCNH_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20525">
        <property type="entry name" value="CYCLIN_CCNH_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031658">
        <property type="entry name" value="Cyclin_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16899">
        <property type="entry name" value="Cyclin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0195">Cyclin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="4">
        <location>
            <begin position="58"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="281"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="299"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2078380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="364" mass="41161" checksum="43F009B9D3D80EB6" modified="2022-05-25" version="1">MLYPTSTQAKNWIFTTEKLKATRDDSHERAKKKFDNAATSAYILTLEEENQLLHHYKSKIIDIGTSLNLPEKVTATAIVYMNRFYLKNSVMEHNPRSIMASTVFLACKTEDNHLEIDYYSQSVKTPPADITNLEMIILESLEFNLIIYHSYRPLYGYLLNISENSGLYGPISFDSFWEHSKKLVQKSLFSDICFFYHPNLVALAILDLTCLANQGSSHTPQQTSNLLKSYMENKLFPNKSPEEHVNITNQINEIKQAILAIPDNPDVNILKEIDKKLRLLSKKAKKEPTSKAPKKKQKTSSTSTPTPNTADTTTTNIVIKSEPPTEQTTTTANTQINSITIKTETIETTETTVNTTPNDVTMQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3L9</accession>
    <name>A0A8J4Q3L9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000001">
        <property type="protein sequence ID" value="KAF2078695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3L9"/>
    <dbReference type="OrthoDB" id="21669at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="195"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="215"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="257"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="413"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="443"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="499"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="546" mass="63838" checksum="1CDC4D897A1134DF" modified="2022-05-25" version="1">MKNNTSHLKALQAENHRKRSVRKYLCKKVVANRVEQKRQEEERQQKMQSELDLKESAVVVIDEINSEEYYKTKSEKITKKKYDLLKKKEEITIKMNALDDKVSQLNTDKNSLFLLLKTVLNAVQKKEAQKLLDTQMSKNHLDNNNNNRQLQQEGSQTLLPNPYNFTPQNVFIRSSNSLISQQQKPNSYNELQNYQQQEQQQQQQPQPQQPPTPTNPISLIPSSPLHHSSDNNRISMPSSNSNQYKNVLSSPSMSPYNNSNNNNESNNNNGHFNFSSNHNNDNSNLSFNNSNMNNMNNINTNNNINGNMNTNTNNSNNNNNNNNNNNNNNDMNNNNNFNNSNNNNYNMNNNFNNNNSNFNKMNSNNNNSFGNNNLNNNGGYNNNNNSFSNSNSSNHNNFNNNNFNSNNSNYRDNYNRGEKMNKNNHGYEKDSYYNKRNRRDFNNYDSDNNNSNNNNFNNNYSQRNNYYNNNNNNNNNNNNNDMNYNNRNINRDSYDDSLDNNYNNNNNSNNNYDNNYNNNRTNKNYNNRSNYNKNSGYNNNNYNNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3N0</accession>
    <name>A0A8J4Q3N0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_000529</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000010">
        <property type="protein sequence ID" value="KAF2078145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3N0"/>
    <dbReference type="OrthoDB" id="10641683at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035301833" evidence="3">
        <location>
            <begin position="25"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="576"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="619"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="639"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2078145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="690" mass="79148" checksum="583DE18B8D12B3C8" modified="2022-05-25" version="1">MRRIAVVEVVCLCLLFVFLNLTKANDLYSAVYQSSNPLWTFDINWKPNQEKFSIPLQNIIITEKGDDNSVKYWRPLKQSTLEIGRAKEFDLEFTFEFIGNYRNAYDWVRNEVEYKGTKSCVETSNRYCCPVDKYDPGYIESHGLEFNSTIYQVYKLSKKSVFPKFTVSLNGVESKITDLSLNDQFVDSNYLVISLSDNSNTIGKMFFMPINMFGDHPNQVGIKKETFKALGIGLCQKAFGSFTSVGQSTNPYDQFDSFTSNTVENVFKRCSFKETILDKSTISFNCLESPTTTIDSSSLVSLSFKISDFPVTQKGGLPFKMEKGLLDKSPNDDDFVYKRIYYDVTNQFSSAVEFTIEPVKCCNGLDCSSFVINKQKQTDLIEGGQKYRYEFTIKSTLNIKYIQDYEKICVFNLYQHGIFIEMVESSFSKDSNQEQQDIRNHLELHHYRSAADNLLNSFDAIQSSCTIDQTFNSTLGTCVPVKCILKYRNSDKIYYNQKTGLCESVQSCPDGYILEENVCVNKTKPEEDPKPQGYDDICKTANDCVDSDVIDTIDNTNVTIDPSTISSKPGTSKQSIITIIIFFVVIVIVLVLLIVIINKCCLKKNKKKKYFNQQQQFNQQQLNQQIRSPNTNVRRPRVVRNNNNNNNNNNNNNNNNNNNNNNNNNNNNNSRANRIREMRQERMKSMGSFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3W0</accession>
    <name>A0A8J4Q3W0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_001051</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TALE/TGIF homeobox family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000023">
        <property type="protein sequence ID" value="KAF2077664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3W0"/>
    <dbReference type="OrthoDB" id="20948at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000059">
        <property type="entry name" value="TGFB-induced factor homeobox 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="435"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="437"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="164"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="299"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="336"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="204"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="241"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="306"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="353"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="378"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="397"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="407"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2077664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="517" mass="58136" checksum="40E4640C6508AC52" modified="2022-05-25" version="1">MKSAYYNNNNNMNQDSNSSRTLPPLVVPPNIKSLDDSVLKSFTKSIYNNSNDLIKPNRNNNDNNNNINNINHNNNNNNNIKLNNKMELIPFDQIVNIIIELESLVNNQNNQNNNQNNNSNFNRQMINQLIQVLKQQLEFSKNSINNILNSKDCEILDLKEKINNNNNNNSNNNNNSCSNSHNNNSNNNNSNPLKSSKDKFRLFNLISNNNSNMNNNNNSDHSTPSSPKSSLNDSQDGFSSNRHHPYLSPNHHSHHHNNNISNNSKAHILSLLDDIDSESSNISSDSDDDCDRKILNTTTIITQPPNNNNNNNNSNNNNNNKPKDDWATEALLRLHDCSNNSTPGSTPPHHSELPSTNGTSQPSSIDPFKNNTLQPPASLSSSLSSSSSSSVTMYPTNNNNNNNNNNTQSKPNPFMSSDDSVNFTNTSIEDAAKMSSKKKKRGKLPGEATSILKKWLFEHNMHPYPTEEEKLTLANSTCLSFNQINNWFTNARRRILPRQLDRKVFGSPLFSHFSIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q437</accession>
    <name>A0A8J4Q437_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF7906 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_000859</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000018">
        <property type="protein sequence ID" value="KAF2077814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q437"/>
    <dbReference type="OrthoDB" id="18451at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057228">
        <property type="entry name" value="DUF7906"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31515:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31515">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25483">
        <property type="entry name" value="DUF7906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035323481" description="DUF7906 domain-containing protein" evidence="3">
        <location>
            <begin position="28"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="733"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7906" evidence="4">
        <location>
            <begin position="40"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2077814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="770" mass="87761" checksum="0089D1FEE439567A" modified="2022-05-25" version="1">MMFKSTISILAIVFVLLLQTSTNTVSGEQVVIEEEPNGIKVKVQVFLLGFNGDGAFNYKLDGNELFNLLKDTYPLHRPHSVEKSNYLKTFDPNNDSSNNNNNNNNNQQQDSNALMNHYDIKYFVKSLGSQSLESYEHLLRSNLERLPFNPDLEQEDETYDNETHLIAIEKLESFFEYEIKMNTQESGLVYTIFFVNPSKDRVFQSEPQLKIKERVVPNKYHYSLNKQICGPAWIGSSRYLVVDLEAGPLKYGSTIHKSTDERISEGSVDYDSLPRLVEYFLESGYQGTSLQGSSPIEIEAHMSNVVISAVRHVFFQDTQYDYIPLFPKILIPILIFKDHTDPVQADIELDIIRKEANRIFPFSQVEIVIGEHALHEHKTISMALYKSIRSHSTYELNPFLNTFQPNTKTYVDSKELLLKLKNEDDILALGIIGDRVTQLPISLNQNNNHNHNNKKNTNLQKTKILPVYIFSLLTTPMNLLLDKYYLYSSNQDSIVILQTKFTFQGTFYQGNKLVSVNAMKTINRQIVAGLANSQGIMGPTLRYSEPHQRLVNNYLWSYGQHPFGFFGNTSEISQIFIDSIIRNTIITSVQSSNQNLNLALQKITDFSTKYLIDSLGYDVDDVPYGGNLIDRLYHTPPSKTPIIKGVVKRLHDNWEEILKQMEITLKEMPFISDNQRQLQSSQKEEISKNLLILSTKTLGFLEYVKQELDTVESQLLCCAIAYALPSSNTYSKIIYFCLVSFGLGVVGLCVTGLSYLSNGGSKGKKRSIHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UNZ2</accession>
    <name>A0A8J4UNZ2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010137</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000941">
        <property type="protein sequence ID" value="KAF2068536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UNZ2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057709">
        <property type="entry name" value="DUF7949"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF29">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25820">
        <property type="entry name" value="DUF7949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7949" evidence="2">
        <location>
            <begin position="10"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="1">
        <location>
            <begin position="669"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="744" mass="82857" checksum="19F55655EC5FC5CB" modified="2022-05-25" version="1" fragment="single">MFPFRQSLACPGKPEQCGGPSKGQCINGECVCKSPYITLDCSSQIIIIPEQPVDPVKPNRNTTTEGNLPNGSIQLVPVTRPTNLDEYGYSACQYVYTVILIDTVNTISIVSPTTSQFISNLDGNQMFSITFNQVFGDRGILSLTAVANQVETTFTLDNFECLEIPPITMSLNQQMYYSLNYNTLLWIAKIETATPMTKYLPGKDCRMKRVSGPVNFYSLYYSENICVLPMTLVGVDLNTPTIVNFNVSISQNVNIVLTTPFTQDPFVGSIDAIASTGTTDFKFIVDYKDNVNMNPFFIDSYLMTSDQGNAVNGTIYYSKNYKGGKFYGANQDCPISVFENNNQEVKATLQGIILPNSALLFSNDISSVAAVASDPNAAFLKIIYKLQSNFYDYRNTITVSLKQSISRTYVSFPFGYSSGNIQSGYTMSFSYITSSHFANGEFLKGVSESTYVSIDNPLLVPNTVQDPLIESTNILAFDKNRILYSLVMTDTIELVHNIRSSKDLSGFVIAGSESIVGKDGGNIVFFEFFHDPIYKNIYAYSHVGLYQFSILFSVFGSFNLTKIPNIVFLENDIDTTNKTTINTMVLYYDDPGFNLRLARYTSVNRDLMFFHSEYDTSLQAYIIPFIIPPNRKTGYYNYILDIGNPGISYPSELFDVLFEPLRVRSDNYDDIPPLITAMSVLPSSIVIVSSVDDFTELAFVVTISDTYNGLLNGSFWITSELDPYGYNITIDPSMADSGDQYLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPK3</accession>
    <name>A0A8J4UPK3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nucleolar GTP-binding protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_009323</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
            <location evidence="1 5">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class YlqF/YawG GTPase family. NOG2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000679">
        <property type="protein sequence ID" value="KAF2069361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UPK3"/>
    <dbReference type="OrthoDB" id="444945at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01858">
        <property type="entry name" value="NGP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000559">
        <property type="entry name" value="Nuclear/nucleolar GTPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1580.10:FF:000001">
        <property type="entry name" value="Nucleolar GTP-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024929">
        <property type="entry name" value="GNL2_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012971">
        <property type="entry name" value="NOG2_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050755">
        <property type="entry name" value="TRAFAC_YlqF/YawG_RiboMat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089">
        <property type="entry name" value="GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089:SF9">
        <property type="entry name" value="NUCLEOLAR GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08153">
        <property type="entry name" value="NGP1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="8">
        <location>
            <begin position="207"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="534"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="469"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="11"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="542"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="562"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="625"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="636"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="661"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="702"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="770"/>
            <end position="787"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2069361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="787" mass="89028" checksum="10ADC7848E4D4B79" modified="2022-05-25" version="1">MVKPKQHRNDSFTNRVSKANHQNNPDRITPKGQTNLRSRATIKRLEMYKARPTRTRDGKLISGEFMSKDISHDTRIAPDRRWFGNTRSISQDQLTTFREELANAVDNPYKVLLHASKLPLGLLKDSTKKTQMNLLSTESFQTTFGPKQQRKRPNLKAVADLDNMITQVGQTTGAYDETKDKNIKIEEDLKDTTNKSVFGKGTSKRIWGELYKVVDSSDVLIQVLDARDPMGTRCQHLEQQLKKNSKHKHLIFVLNKCDLVPTWATARWVKILSKEYPTLAFHASITNPFGKGSLIQLLRQFSKLHTDKKQISVGFIGYPNVGKSSIINTLKSKAVCTAAPIPGQTKVWQYITLMKRIYLIDCPGIVPTTGDSEAELVLKGVVRVENLEDPTEYIPEVLRRIKKDYIVKTYNISEWTDHEDFLTQMAEKTGKLLKKRQPNMKACAKLVLYDYQRGKIPYFTAPPEITKEEEEEDLKIKQEQEELDKIKQEAADLAAAGQEAEAKEETILDKNKFVGDLVVNQKLKKIVVKSEFNEEDAKGDEYVDPFDEEPDWDELYQKDSDDSSDDDDEEEGDDQEDDDEIDVKEEQELDDEEILYGAAAEDDLAGGDIDYDEQEKSKPIRNKKKAVESPKKKETPVAAPKGKGKAVAAPAPVAAKKKETPAPAAPKAKTPAAAKKSKKEPVAEPEEQEQEQEKPKQKVKKTVVPTPKVTKATPAPVATNPKSKTNRKEYVEVMPEVESDTEAGPGKDKRKSTNKQKVGVHFYETANVKNRNRDRKGPKKPKYQQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQD9</accession>
    <name>A0A8J4UQD9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008136</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000477">
        <property type="protein sequence ID" value="KAF2070546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQD9"/>
    <dbReference type="OrthoDB" id="270602at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02958">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01767">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006577">
        <property type="entry name" value="UAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050730">
        <property type="entry name" value="UBX_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322:SF6">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13899">
        <property type="entry name" value="Thioredoxin_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00594">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="2">
        <location>
            <begin position="375"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="286"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="302"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="453" mass="52276" checksum="B0348F6845889D27" modified="2022-05-25" version="1">MDNEEKLQIFLATTGSEKIDEAWNILEQNDWDVDTAVNFYFTTLDDPQASSSSKAYSNSPPKSSSPPPVMYDDEDFVRAPIQSKMDTLVDYQEPIARPSRYQKPTNAFEAFRDFEKERGINKDLNLTGKQKTLAELFKPPLDILTFGSFTEVKQIAEKREQWVLVNIQDVSEFDCQKLNRDTWSDKKVKEFISENFVFWQVNNQNTEGKWFIQYYPVAVFPFIAIIDPRTGQMMKSTTGFVEGEDMLYYLQAFISANGYNGEKSMKKQKKYVTEEEELEMAIQLSLKQDEKKKNSPPITPPQTTTTTTTNNNNNNNDNDNDKKEDDDDQDFDEDDKYNVEDEDEDVYNKIIEEEKAKIKMIKEASEDPKIDSKIGLEGDCSIQIRCPSETIKGNFNSTDKIQVLYYFAQLKTGLTNFKLCTSFPKVELSGDLMFKTLKEMDLAPRAVLMLQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4USU0</accession>
    <name>A0A8J4USU0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ILEI/PANDER domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004688</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000170">
        <property type="protein sequence ID" value="KAF2074011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4USU0"/>
    <dbReference type="OrthoDB" id="20902at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047223">
        <property type="term" value="F:beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016266">
        <property type="term" value="P:protein O-linked glycosylation via N-acetyl-galactosamine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039477">
        <property type="entry name" value="ILEI/PANDER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052463">
        <property type="entry name" value="O-linked_mannose_GnT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46396:SF2">
        <property type="entry name" value="ILEI_PANDER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46396">
        <property type="entry name" value="PROTEIN O-LINKED-MANNOSE BETA-1,2-N-ACETYLGLUCOSAMINYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15711">
        <property type="entry name" value="ILEI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ILEI/PANDER" evidence="1">
        <location>
            <begin position="34"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="ILEI/PANDER" evidence="1">
        <location>
            <begin position="188"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="ILEI/PANDER" evidence="1">
        <location>
            <begin position="368"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="ILEI/PANDER" evidence="1">
        <location>
            <begin position="520"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="ILEI/PANDER" evidence="1">
        <location>
            <begin position="696"/>
            <end position="786"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF15711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1122" mass="124162" checksum="A7291D3DB9AA0166" modified="2022-05-25" version="1">MPIVIKLCSATTVQKSFIKVLREDQYYPITANRINMWVMDQKELLFPFTFNSFDVSSAANVQSFMNYVDNIKIGCSVMVIIVGDLPIPQTYKSLVYSTFQYFGGSKISNVSATNPNYCLIGYKGQPVGQATEVFGKSTDSLSIEIEMEIAQSKSWNTKTIQITTYNSYSNKPTLLIDGRPVSLNISTGMNVITFNKYNQVETIKTFNTNAPNPAPNPSNPEPTISEQFSDFLDSIDESALTLIFTYGETKYNFTLDNVNQIKKFGSKFISDFYNNANNKCWYLFGRKEDVNSVIEATTTRDISVGYYFYTVVDDNANYQYENDDDGNIYLSVFSNNTTAVTSYNNLFIIDDIDLKSNKSFYGFVLGDVDENDGTLSMLQYFDIPVNGNEQLTLTSVQSLVDAINAIPTGNLAILSASKLPSGFTTPSTLTNALYTLGSCSGSGITGASSFSLIGRKGSPSGSAPEDISTIGPVSVCSNFNFKGTLVKPFIDIKVVSYNNKSGESEFYINGTKVPFINNTGMNVLVVDSFSGTISYTRNFNTIESEESDQLSQFIESLPVGSIVGLSSQGTLNLNERAKKTISNYFKGDIKVHQSESNSIYTLGPNDSYCSIGQILDKHSGSTSFKPLYNESFNSYVDGNGEIKTTASLRYPLKYLFDKVEGHNINVQAKSGSNALAKIEIDGSIVIGENGSTPSIDGLNIVTIRPDEITPFVHQFNVIKDQNEWFSFFNFINNLSKSTLVIVAVQEEFGKISDPITEYYMKSAFRMIGGSLFDDHLSSFAMIGCKGIASSSAHELRCKHSSFISISSWEPKRITKLLSAPFDNKRIGRDAIFAVPLFEEYYKLSNPTSTLPKQLAIGHKYSIPPAFSQSPRPRIGATPIKRALIVSCSYVKSLSGRLSDAEYHAQQHAQALITAGYLTRDNIKILSESTTEPSPSGYIGPNLNNIKSQINWLQSEIVAGDSIYFCFVGRGWTSMPWGRTEEDKWYGGLVLLTQSYVSLEFFYFHAFQALFNKVVEGINLSYLFDCDYSHELAVYNSGPTPPYPWNINAISSLAAGKMKTNSQLSLSFLECINTAISEQYNNGGSKLTYSQLVNKVLQNPSYLDQSPTYEGPNPNLTFLNPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UT45</accession>
    <name>A0A8J4UT45_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004272</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000148">
        <property type="protein sequence ID" value="KAF2074416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UT45"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="195" mass="21671" checksum="92E3E17AFDA95B89" modified="2022-05-25" version="1">MNFIKNIIKVGSSFFNNGIKNTNISGLSKSIFQEEVATVATGPTHAETAKERLKFMMLENKKREFTLTPQQQQQQKQQQQDAVITTAIESRDDMVSRELDSLSTTKSHSNSNLESTTIIATPSTSSLNNNHIAEKKQELSSIKGVGGVTLSFLNNNGITTIAQLSEITDEQCTLFKSKVKHINNLRKEAQRIISQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UU66</accession>
    <name>A0A8J4UU66_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA mismatch repair proteins mutS family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_003611</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000115">
        <property type="protein sequence ID" value="KAF2075091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UU66"/>
    <dbReference type="OrthoDB" id="295033at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032301">
        <property type="term" value="C:MutSalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03285">
        <property type="entry name" value="ABC_MSH2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.110:FF:000002">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000523">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1420.10:FF:000105">
        <property type="entry name" value="DNA mismatch repair protein Msh2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011184">
        <property type="entry name" value="DNA_mismatch_repair_Msh2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032642">
        <property type="entry name" value="Msh2_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003810">
        <property type="entry name" value="PRK05399.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF35">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005813">
        <property type="entry name" value="MSH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="DNA repair protein MutS, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="10">
        <location>
            <begin position="757"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="486"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2075091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="914" mass="103016" checksum="DFB8A1052A17477F" modified="2022-05-25" version="1">MSDTEDSSSQTTTQAVVLKEDKGFVSFFQTLDDTDTNTIKLFDRKGYYSLHGQDAVFVSLMHFKTKKSLKYWTIEQAKKKLKTSNTVVHSKSNNEDDEEQEDGLAYLTIRQGVEYETIIKELFDEKKKIEIWGNRPNRANQWEIIKKGSPGNIQMFEDVIQTNIEGSCMMALKIAREKGTLTFGIAFGDATFKTLGLSQFVDNDHLSNLSSFIMQMSIKECLIYNDLKSYDHKKVTEKLEEAGIPITIVPKADFNSKNVDQDLTRLLGNISNNLIDLEQEHAIQSVSCLIKHLELLSNANFFGKFKLEKFDLEKYMRLDPSSFKGLHIIESKEATKDQSLYSLLNQCNTPMGSRLLLQWVKQPLIDTDEIEVRLNFVDAFYNDLELRESLRANDLKKVGDLDRLAKKFYGQKSSLEDCVNLYGTTQRLPVLLSSLRNYSGTYQEILQINFVEPLTGMIQGFKKFAAMVETTIDLDLANDKHEYVIRATFNEDLKKMQDKKNEISKKIESTRLNVSDAIGLDESKVKLHYTDKDGYLLRISRKDEPSIRKNTKCIVYATQKDGVRFSTKELGMLNEAYTKISAAYQEKQSGIAKRALEIAASFVPLIEDLSSLIATLDVFVTMAHVSSISPIPYVRPTVHPLGQGSTEIVGGRHPCVEIQDGVSFIPNDISLVRDESQFQIITGPNMGGKSTFIRQVGLIVLMAQIGCFVPCQEASISVVDCILSRVGAGDSQLRGVSTFMAEMLETSYILKVATKNSLIIIDELGRGTSTYDGFGLAWGIAEYICNQIGGFCLFATHFHELTVLEEMMPMVKNLHVAATADNNTFTLLYKVEKGSCDQSFGIHVAVLADFPKEVIDLAKLKAKELESFESNTLKKNYNQFLDEFKEIDLNNADQEKTLSLVQNLLSKYSIDINN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWD9</accession>
    <name>A0A8J4UWD9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Exocyst complex component Sec3 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_009099</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000636">
        <property type="protein sequence ID" value="KAF2069585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWD9"/>
    <dbReference type="OrthoDB" id="27109at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048628">
        <property type="entry name" value="Sec3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019160">
        <property type="entry name" value="Sec3_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16092:SF14">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16092">
        <property type="entry name" value="SEC3/SYNTAXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20654">
        <property type="entry name" value="Sec3_C-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09763">
        <property type="entry name" value="Sec3_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec3 coiled-coil" evidence="6">
        <location>
            <begin position="109"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex component Sec3 C-terminal" evidence="7">
        <location>
            <begin position="477"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="425"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="427"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="439"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2069585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="799" mass="91489" checksum="2AA77A8183291104" modified="2022-05-25" version="1">MGDYYYSEHKRNSLTITPELKGSQGFNQSGSLYMDDNSNNPRITSTPYQSNIQAIPLPDILAVGNNKYSNSSPTSSNTTTTSVAGNIDFNDDDLEMVLQNYLSSETDVDLLTEKLGKQLSTLETEMVVGILDSGLGVNEIISHLGDKDETHLTGVTAWIEYYNKQLQDMKKYIEHIESKNNKMEVVSRNQNSLLKELDSLLKLLTLDDKTIKTLTTPDFSTSSGLENAIAASAQLKKALTTKLKSGMENMVAVKDQRKQFETYKISFSRKVSTLIESIFKVDQSKDYQKVVGEELPEHTGFYHILNSFKPLMHWLKEMDADKYPPLVAIYIKSYRSAYKHENKDFFSALGHSIIKESKDQSDFFSSGKKTVENVITGAPTSTPTKSNVKKRTIDKVFRYCLSCLETSIMAEQKFTMEFFQFHDPPRMPKKKHHHHHHNNTTANTPPTSTNTSPDQISPPPPAAAAPQQEETSPDLALDLILSEMFDGVVPELRDLVEKANDINPFYLLTMLGDTEQYISSHSQLGAEHSSFIIKILSEVQKTMKSLFNKFIDVQVEAIKATQSSLKRCGVLSHFKHFPIFVKELEKYRSKNEEESITSLINSSYYKIVVNLYGWLDSLVEKLPSDQKYRFISKLENYYFLTVKLEELNIECLLQYREQSLTRYKENLDVYTNYLIGLKFQPLMDFYSKMDELLLTLPPSDIQFQQSHSKQQYKKMVEKFKTENIEKGLLKALSNIYKNITKDSSLINVVWGHLEDVFIERYDHFQDITQQCYQQTIPVNSDQIRGLFTNILKKNPNKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWS3</accession>
    <name>A0A8J4UWS3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008643</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000553">
        <property type="protein sequence ID" value="KAF2070035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1924787at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1343"/>
            <end position="1366"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1062"/>
            <end position="1073"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1394" mass="155366" checksum="F8836A916A0CF937" modified="2022-05-25" version="1">MLKLLGVYPLGTTPLQNSIFGELKCTGSYYILLEDPDHLVGSLSGGEIVSEIDHGVFLWKYKITISPSHIQPQQATYSVQLKNSTIVHFSAQQGAFSCPANFSTSIQSPNPAPTPNVIGQPFYSLFSDTVNLIVYFNNTSPINLIYLNSLNALGTSASIFNLPKPLPSNATAAPYRDANGKPTLSHMKPNLPLKGIETYGLTVKPVVYKGALSAYHIQFPASSYARDTINSVSVNLTFQNELLNRSTISIPFPNSVSSPSIAWSNFRFYPSISDVTLVTQDNQPISILIIFNVKKTKDCPMSVSNGVLFSLERPDEGLYAIHYRTSKSSKITLSSPFSLDTKEFNIEISNSIKSNIQANPLAGNLVNSPNNPFTTSFVLDRSIQVNSEIPVHIDISSTTNIIHYDCYFPFGYVSGNSKKNYTLGISHNLYHLTPFIQETIYPGYNDPIVSRGNIVSDQVDDEPPFIHNVTVYRLDAQRVTLLINATDNWAGILSINVLGQFELGSEHLVIGTMNDGLYSLLNVAFDQLKYDPSKNDSILVIKDYAGNERMYPFNSFINQDFDPVPALIPFIDLNEIIEFKIDPQDIDPGHNDVDVSVLIKSKTIDRSWEPLIALVVPPYGPFPSIIARGNYSGSNDDFLIRIKIPRGFYPGQVQYYFNYPSWTNQFISGLFPNSPSQTKLNVKYYDEASNKFDFLAPIIKKVSVSPAVEFNATSTNTVSFALQVEDAVCGIKRISVNISSSIDPVIYTFSKNYSNSLSEQVVIDISVGHCLDQTFEIVDVFLEDGCGHKNFYHKYLISKNPPNPYFITPMWISDPLPVGRITCKNPIVDTIAPTLVELDVYPKTLQADTNVVSIRFTVKDKGDAGINRRSKPTVYFQSSTKAPLAYVSVEDNVYNKSCISYSVMARLPYGYGDYGILLSIYGLMDNNINFKGYTSSDLASNSFDSFLLYNSTLITAIKKISPVTTQGGSVTVYGTNLSGKNITAYFRSVDGTFENTSSPLEKSLGQLVFQIPPVNVAALSLSLDIDGVFYTPPIIEILNYVQSIKPCSNNCTNRGVCTERGCECTYPHQGSDCSSIIVPPDKIEYSFGNYNPEGVQKVVAVERTLTSTISLFGIREVDHHNKLIKSYSIAKWHKEEDQLGTTYTGFLDAVRYPSAQFKISVQYYKNPKQLDFAGERVLIDSNSFKFTITSIAFPFSSQLNNIQIILKTSLKSDKQTCYNSTNFGYTNSDETEISWLELNSDDYKIYGKFIKKGIIDDIDTIVRNSQIPPEDPEFKDYIDSSTSLVALYIPYFKTKAVLDPPLALLMSSRDEVNANNNNGDNINNNNNNRDYVCSIPLKTKTNIVIVTTVAVACVLVLFSIILAVKYRWAIKYKSKRLIEKFKERYQNHNKKTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWY3</accession>
    <name>A0A8J4UWY3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_000654</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the akirin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000013">
        <property type="protein sequence ID" value="KAF2078016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWY3"/>
    <dbReference type="OrthoDB" id="10039914at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024132">
        <property type="entry name" value="Akirin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13293">
        <property type="entry name" value="AKIRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13293:SF6">
        <property type="entry name" value="AKIRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="104"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="48"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2078016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="215" mass="25743" checksum="826E0C0E6555DD9F" modified="2022-05-25" version="1">MLKLKRSYDSFSDNVVTTPTPLTINSNIQQCPTIKLVSDQHKRHKHQQQQTQNNNITSSTHQQYSQFFNNVNSPIDDILDKIKNTESIKRPSPPSPQQQKSSSIIIIEQQQKQIEQEKKQIEEQKQKQIEEQKQLELKQQQLLQIQQQIQQQQLFTLEQVQEIVKKALKENEEKLKREYEQIVKDKLIEQYQCFSRYNDDYISKQMKESEYSYWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXD2</accession>
    <name>A0A8J4UXD2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Leucine-rich repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007918</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000448">
        <property type="protein sequence ID" value="KAF2070770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXD2"/>
    <dbReference type="OrthoDB" id="17973at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052394">
        <property type="entry name" value="LRR-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="148"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="744" mass="81513" checksum="034B94D029EC0425" modified="2022-05-25" version="1">MQNYNQMSNVDHQYYYLQGQESHGQQNIDPNIKKEDIDTISDQPYLPPHLQHLHHQHHSEMDLHHLQQQQQQQQHHHHLQHQQIPHHLLQHPQQHISHQHLPHLQQHHISSEQHKQQQELIKTDSDKENRNNDIKLEDPQQIQSIIESTPPTNNNNSNNNNNNINQQNSTSSSGASSTTTTTTTSTSLSSTSATTPTSTNTYEVPEILLIPGVRPPYINSLIDNNILSNILAKLHKDEPTSSAFTAISGVCSEWRKLLTNRIVNYVYQIPADKNMTNLFKNIANNKYPNLVSLQIKVSTPTLFDVSNFVKLLMTNNHCITSLELTQNGIGNKAAQCIGTCLIENTTLTCLSLSFNSIGNEGAEEISKALGINKTLISLDLSQNCIGFKGSKALGQALLATTTLQTINLSKNRFGTKGIDAISESISKNTSLTTIDFSKNDLNEKSCKIIGDAIRKHPTLTSLNFCDTKLSAESMKYICEGIVSSSTISYLDLSRNEFGHKGARSLATAISSTNSITYLDLCGTSIGDKGAQVIADSIAKNHSIVNLSLAFNNIGTGGALSIGNALKVNNSLQILDLSINAEIGYSGFNDICLGLSMNKKLTTLHMGTTGFGPNGARSLAACLKTNSTLTDLQLRGNEIGDDGAKFISDAMRSNCTLVELNLSGNGIGNDGAKALCETFYLNPNLTLVQLNHNTINQTGLSQMKDALRQNSFSVTSDSYFHPPNSTTTVSCLCVTLNQNVLRIIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UY37</accession>
    <name>A0A8J4UY37_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007299</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000381">
        <property type="protein sequence ID" value="KAF2071383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UY37"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35885:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35885">
        <property type="entry name" value="CARBOHYDRATE BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="297" mass="33149" checksum="6833D2E501F6CC7B" modified="2022-05-25" version="1">MGICIWNDNNNTCIGLYDYVNTTRDATVKFTLQGYNYMGGEHTMSAYNYTTKKLSFVAIKVGTTERSLFTISTETWTQLNALVIPDKNTWEGLQYDQTDRADSLLNAGNNGQGTIFLTRLNPMVTSFSIFGSIVGSLLSAAYDTKSFKYHVAYKHGEDVFINIYNMAANPVALESSQKYSFTNIASRFSYASGPNHMYHHPKYNSIMGIVSLRDSHLPNPHNSVLTNITAANKEIRITGMNSDPRHVVTACVPDQTGAGYIYTITKFSDKLYCNTFSPYNNTCTKNVLINTPILALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0K2</accession>
    <name>A0A8J4V0K2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Iodothyronine deiodinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004160</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000142">
        <property type="protein sequence ID" value="KAF2074533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0K2"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004800">
        <property type="term" value="F:thyroxine 5'-deiodinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000643">
        <property type="entry name" value="Iodothyronine_deiodinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11781">
        <property type="entry name" value="IODOTHYRONINE DEIODINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11781:SF22">
        <property type="entry name" value="TYPE I IODOTHYRONINE DEIODINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00837">
        <property type="entry name" value="T4_deiodinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="130" mass="15078" checksum="F98387DFE3D4237C" modified="2022-05-25" version="1">MSMLQGIMDQFKEHVDCYIVYLKEIHPADEWYIGGDEVSVCYKQPTTMQQRVNIVQALQEYAPFVKVPFLVDLMDNNFNIGYDAVPERLYVIENNKFSYIGGPGPFFFLPEDLVKYLYQRFPSSSSTPAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0X2</accession>
    <name>A0A8J4V0X2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glucosidase 2 subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_003722</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000120">
        <property type="protein sequence ID" value="KAF2074983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0X2"/>
    <dbReference type="OrthoDB" id="28322at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017177">
        <property type="term" value="C:glucosidase II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039794">
        <property type="entry name" value="Gtb1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036607">
        <property type="entry name" value="PRKCSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028146">
        <property type="entry name" value="PRKCSH_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12630:SF1">
        <property type="entry name" value="GLUCOSIDASE 2 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12630">
        <property type="entry name" value="N-LINKED OLIGOSACCHARIDE PROCESSING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12999">
        <property type="entry name" value="PRKCSH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13015">
        <property type="entry name" value="PRKCSH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035206189" description="Glucosidase 2 subunit beta" evidence="6">
        <location>
            <begin position="24"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="7">
        <location>
            <begin position="304"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="143"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2074983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="409" mass="47181" checksum="964A9B55A73DD3CD" modified="2022-05-25" version="1">MMMRLNILIVSSLLFLIIGLSYSKPLSKDYGVSPEEIEFYKGEKFNCLRSNKQVPISQVNDDYCDCPDGTDEPGTSACSNGHFYCINKGHKGQFIASSFVNDGVCDCCDGSDEYSSMMKCKNTCNELGKEMREKQLQDQEKYAQGLKKKAEMIKDAIEVMESKTASLEKLRKEIEPINQEIKELEVLKENKDKEKQQELDKIKEKEEEFKAKTTLETTNEEKQQQDIPDNDVVKEIEKELKENEEKEQLTSEVYDEILKGIEQLEKEIQTKRDSLRDKQNEIQKIEEMLKMDFGKNNVFLALYGKCFDLKTKEYTYTLCPFDRANQGGTSLGKFEKWENDYENMLFTNGQQCWGGPKRSIKVSLECGSENEAYDVQEPGKCEYTIKLKTPASCDEEHLKVLLLENDHSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V217</accession>
    <name>A0A8J4V217_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_000911</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000019">
        <property type="protein sequence ID" value="KAF2077790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V217"/>
    <dbReference type="OrthoDB" id="10249039at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019788">
        <property type="term" value="F:NEDD8 transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23794">
        <property type="entry name" value="UBCc_UBE2F_UBE2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000005">
        <property type="entry name" value="NEDD8-conjugating enzyme Ubc12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="9">
        <location>
            <begin position="80"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="17"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="63"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2077790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="233" mass="26243" checksum="302B404441936EDD" modified="2022-05-25" version="1">MFRIKELQKKKQEQQKQAEAAANPTNGTAPAAEGETPATDVKRQNSNDLKEIRRQKSKDSYFSLKTKQSSETGSTKRANPAELRAQKDIDEMEVPTGCKVNFKDTNDILNFTLDITPTDGLYQGATFHFTINIPTTYPYDPPKVHCDTLVYHPNIDLEGHVCLNILRQDWMPVLNIGTVIFGLMTLFLEPNPDDPLNKEAAQLMIDNRKSFEANVKQSLRGGYIAGRTFPKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V537</accession>
    <name>A0A8J4V537_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004468</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000158">
        <property type="protein sequence ID" value="KAF2074222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V537"/>
    <dbReference type="OrthoDB" id="16239at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035203342" description="AB hydrolase-1 domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="124"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="377" mass="41665" checksum="EDDBDBFDE2A90F80" modified="2022-05-25" version="1">MKSISFISVLLLLFISTISSTTTSGTPEPSCFCPIPASASSESVSTPSVTLSPPLPSPTYFPGDWGTSYYPLFDGLRMHFRCWVNPNITSGETMILDSGLPFFSTSFSAVIEELIPVMTANSIKRACVVDRHGYGWSQPAPYPFDSFESVRRMRGSLNAMNLCPPFVLLGWSWGSIDVQTWAAYYPSEVRGLITVDGSDIGMLKDPSWDTTIPNLQTLFSSYIVQNNNGLLRDYGDDGFIDEFYGWIKPTANIPTGTITKSQDIFVHPSNRYLTTSKQEFSIMMSSADFLNQTYEAATTAYPLLNIPFFIYTCGTNGVDWVNRHNAMALLSSQSSHHVDYTTTHLIPLEHANLVIDDIIVPFFDLLALSSPTSATTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5A7</accession>
    <name>A0A8J4V5A7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Arginyl-tRNA--protein transferase 1</fullName>
            <shortName evidence="5">Arginyltransferase 1</shortName>
            <shortName evidence="5">R-transferase 1</shortName>
            <ecNumber evidence="5">2.3.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Arginine-tRNA--protein transferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_000692</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the post-translational conjugation of arginine to the N-terminal aspartate or glutamate of a protein. This arginylation is required for degradation of the protein via the ubiquitin pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>an N-terminal L-alpha-aminoacyl-[protein] + L-arginyl-tRNA(Arg) = an N-terminal L-arginyl-L-aminoacyl-[protein] + tRNA(Arg) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="Rhea" id="RHEA-COMP:10636"/>
            <dbReference type="Rhea" id="RHEA-COMP:10638"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:78597"/>
            <dbReference type="ChEBI" id="CHEBI:83562"/>
            <dbReference type="EC" id="2.3.2.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the R-transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.8" evidence="5"/>
    <dbReference type="EMBL" id="AJWJ01000014">
        <property type="protein sequence ID" value="KAF2077968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5A7"/>
    <dbReference type="OrthoDB" id="74183at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004057">
        <property type="term" value="F:arginyl-tRNA--protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017137">
        <property type="entry name" value="Arg-tRNA-P_Trfase_1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030700">
        <property type="entry name" value="N-end_Aminoacyl_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007472">
        <property type="entry name" value="N-end_Aminoacyl_Trfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007471">
        <property type="entry name" value="N-end_Aminoacyl_Trfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21367">
        <property type="entry name" value="ARGININE-TRNA-PROTEIN TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21367:SF1">
        <property type="entry name" value="ARGINYL-TRNA--PROTEIN TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04377">
        <property type="entry name" value="ATE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04376">
        <property type="entry name" value="ATE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037207">
        <property type="entry name" value="ATE1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="N-end aminoacyl transferase N-terminal" evidence="7">
        <location>
            <begin position="20"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="N-end rule aminoacyl transferase C-terminal" evidence="8">
        <location>
            <begin position="294"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR037207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2077968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="540" mass="62442" checksum="86CF6B71C6551C9D" modified="2022-05-25" version="1">MLIGGRKQSVVEPYGYYGGECHYCYESDDGRISYGMNAIQLTVEDYQSMIDRGWRRSGTHLYKPDNSNPKACCPQYTIRLDANRFIPTKDNKATCKKLENYLNNNNNNNANTKNNSDNNNSISKNIKKSVESLPSSIKELNESILETTISFLNDRKIETQEEFLKKTLQIKANINKLVSTRGNYSICFSILNKFKVDDNLKQELIENIINTTTIDNDEYKLFGEKDHINIYLSQQQNNQADQVDQQEVEGVKQAVVNTKVNNKPDTTTTTTTTTSTTTKKELKITIEKAECTDEIFSLYCKYQKEIHHEDEKKEKKGFIRFLVDSPLIKIPYNQAGYTFKDKDNQNKTIPIPQCGYGSFHQHYRLDGKLIALGVIDILPHCLSSVYFIYDPDYSFLSLGKYSALQEIKWVQNISKSIPNLNYYYMGYYIKDCPKMNYKGNYQPSDLLCPETYQWVSMEKALLCIKDKKYSAFVIGGDKKENKKQDVDNKAIEKVKLLYNYQSINFGDILPKYQKYFKDKIIDYVRNLGPHLSSEILMTVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5F2</accession>
    <name>A0A8J4V5F2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor Iwr1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000147">
        <property type="protein sequence ID" value="KAF2074447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5F2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="282" mass="33388" checksum="904F7C10BA803B25" modified="2022-05-25" version="1">MIKIKNKKKIKKVQSEKEGLRKVLKQRNIERKNWGESDHSEDSEDSEDLRSEYENHNIQVDSFKAYKLWAQDRDCLIYDKVLVNPSEHFAANVVHRKELENFKVDPLKYTYDQQHQERMNNIPNLNSATIDQDCLEMGYTEPLEEEVHICKHRIELLNQKMAKLTPYPGSELTQISLPKPSKIESDYESSEVDSEFDFDFNEDDDYYRLAYPEVTSGEEDNHHGPPSEDDDDDDDDGDEYFDDDDDEYFDDDDDEEDSDEARERIMEFLFQLSGRSRASRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5P7</accession>
    <name>A0A8J4V5P7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Actin binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_006634</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the formin homology family. Diaphanous subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000315">
        <property type="protein sequence ID" value="KAF2072044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5P7"/>
    <dbReference type="OrthoDB" id="1104827at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005522">
        <property type="term" value="F:profilin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070060">
        <property type="term" value="P:'de novo' actin filament nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010472">
        <property type="entry name" value="FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051425">
        <property type="entry name" value="Formin_Homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014768">
        <property type="entry name" value="GBD/FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010473">
        <property type="entry name" value="GTPase-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725:SF1">
        <property type="entry name" value="DISHEVELLED ASSOCIATED ACTIVATOR OF MORPHOGENESIS, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725">
        <property type="entry name" value="FORMIN HOMOLOGY 2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06367">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06371">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01139">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01140">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51232">
        <property type="entry name" value="GBD_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBD/FH3" evidence="6">
        <location>
            <begin position="10"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="FH2" evidence="7">
        <location>
            <begin position="542"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="453"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="514"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="845"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="950"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="401"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="453"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="465"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="521"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="950"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="978"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1050"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1065"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1094"/>
            <end position="1136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2072044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1150" mass="129420" checksum="F69C623CCCF7EB5E" modified="2022-05-25" version="1">MVKLFGKSKKKEEDPSSIDAAFQSVLEEIGVPESEKKHLMTMDIARKQQLTQSYQSKLIAKKEFGTKSKKKSVVSQSPQYFVDGLKAEPTKELLTCLRVRLGNQPLKWLREFINIEGVSLLIKVLIDNEIKQVKNQEDIYKIAQCLHSLKLIMNTKMGLDSVMKIPNSINSICLTLDTSHLKTKIMISELLTALCIVHGKGHALVLSGMDNYREVKRERKPFLHLIQGLKNTSGSLQSVTFGLINTLISCSTDVNERTKIRSQFKKIGLVQIIQELEPEYANNPDLATQRDLYEQESRWDEQEIIESVRGDVSDDNPESIFRAIRERTHGEPHSPAFVSILKLLLKSFSEDDSTGYGTSLSNYLFIEKILNKINGGEVITDDLGEFFGGGMEINHVSGEKAVLIQKEIEDLKKQKKKDSEKLHEKDILVTKLAKRLKRLEEAVKKGGLHIDEEDSFDIPDFEGDGFDHSPSKIGDKPTISKTAPAPKKDLSFNTVKAPNESSNFLSGIETEAASASGAPIPGGPGGPPPPPPPPGKMAPATPQLCSRPPNVKLKSYQWSKYRTRNVQNTFWKMVDHAKYSECLPYEQIEFLFAAAVIEKKEKELKKGSEVTVIDPKRAQNVGILLSRFKNFNLDQIRDAIINLDDSILDLETINQLVKYIPSKEELDAIKSFKSAQESVEETERMRLGKAEVFFDKLADIPRLAQRIQALHFKLNFPEKLYQAKPDIRKFNEGLSELQNNNIMSIMELVLSIGNFINFGTNRGNASGFAIDSINKLADTKSNVREKYSLVHYIIELLEKTQPKLLDFPSEIPNVIEAATLSYNQSCNEIKLLRAGLVKLQKEMFGDSNNSANQNNDEDDSGGEKPQPINEPLKDDDPLKAKLSEFLLASKTEISDAEKLISETEALYQKVAKFYGEEPGKVPPEEFLAIFKRFTDTFVLSLKDIERENAMKERADKRKQDKKSSDKKKKEILSKLKKVASPNGSSGSSKSTDSPASSVSSQDDEDEEAIREYIKAIKDQSSPSSRGDSSETEGMMDDVLNLIRDGDFRTIRRNHLSKKGPKPKRSVIDKPTRPKVLDDTPSSTYSSVSSIYDAEPLDISDEEEDDDEIEEDEDESNDEYDDDDIESDDDEEEDDEQERNFNDSDYSPTNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5W4</accession>
    <name>A0A8J4V5W4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000243</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000004">
        <property type="protein sequence ID" value="KAF2078493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5W4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="529" mass="60640" checksum="FEDC088016614296" modified="2022-05-25" version="1">MTESFYLIWRNRYTRQLIRNILCHNFIINVNKEYLIENHRFLSVFSSKDKLDYNITICFKGDAGDYLNINHINRDVINDVEIDIKSDVDNFDLNEIHDGVHKLSLMIEKRENNPRKNNRTSVATGELPSSITYLNLCGKCQSFAQAILSNLPANLKELELKIDQNSLSGPCIMPQSLTKLKSSPYLNYENLKWFVVPPNKVYKSCMLYIDSMESFEWLFANKWICNIGIDSNMLNILTTQHKLPPHVTKLGLYDDIVLDSSFLPQTLESLTCGYGTPISHLTHLKSLTISSDYPFKLEKGDLPASLQELNLYYNHSFGTDVLPPHLTTLDLYGYDQPLYPNVLPPRLTTLFLNDYNQPLCVNVLPSSLTDLHLDSFNRPLDPFVLPSKLKSLSLQYYTQPTFPPNSLPVSLTSLTIYGFKGSFEQCQPLDNLRNLKVDTLVPSLSTLLTNVKKLSMWVYNKDDDKNDRSGIACLYNTSIKSLHLYFCWLKTLYPNTFPPTLKYLTLVKAHLKSNDVIPRGCVLLKSKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V697</accession>
    <name>A0A8J4V697_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003085</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000092">
        <property type="protein sequence ID" value="KAF2075617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V697"/>
    <dbReference type="OrthoDB" id="332863at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070290">
        <property type="term" value="F:N-acylphosphatidylethanolamine-specific phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024884">
        <property type="entry name" value="NAPE-PLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15032">
        <property type="entry name" value="N-ACYL-PHOSPHATIDYLETHANOLAMINE-HYDROLYZING PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15032:SF4">
        <property type="entry name" value="N-ACYL-PHOSPHATIDYLETHANOLAMINE-HYDROLYZING PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038896">
        <property type="entry name" value="NAPE-PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="2">
        <location>
            <begin position="88"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>an N-acyl-1,2-diacyl-sn-glycero-3-phosphoethanolamine</name>
            <dbReference type="ChEBI" id="CHEBI:62537"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>an N-acyl-1,2-diacyl-sn-glycero-3-phosphoethanolamine</name>
            <dbReference type="ChEBI" id="CHEBI:62537"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR038896-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="329" mass="37618" checksum="091E50E1F2455E03" modified="2022-05-25" version="1">MQKTYSIFKNGKYVNPFGSTNKTFGDMVSYLWNRPKVLPLTEHEKTHVHPVLPVDFDKIASDYDPTTTIRSTWIGHSTVLVQMEGYTFITDPVWSKRCTPLPGFPAGPARYRDPPCSINDLPKLDFVILSHNHYDHLDADAVQQIGNTPKWFVPKGMKAWFKSVNIDNVIELDWWEEATFNDDLTLVCTPCQHYSQRTMTDRYAVLWGSWCVLGKHKRFHFAGDTAKCDVFNEIGHFYGPFDYSLIPIGAYDLNREFMKTFHCNVPEAVEIHQQLKSKRSMGVHWGTYLLSTEPLLEPPTLLKSESAQAGLKEDEFFASKIGVTTDIIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V737</accession>
    <name>A0A8J4V737_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005148</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000198">
        <property type="protein sequence ID" value="KAF2073529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V737"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="686" mass="80428" checksum="86FD3AF2A336E508" modified="2022-05-25" version="1">MDGVSNKDTTTSLFFQVFKNKYLAKTILDHRFVDDEYLSIGMLFKHNRFDLFDQLWGDNGDGLDINHHDLNAFFRYNSCYQRFVRVWNKFRYFISRDKMERWFQLACKGDKDKNILIKMLDQLYEVMYYDFQPFLLLQNLDVPDSVEILEHFFKCRKKKRGQNIEQQPLLDSLEAISFLAHPNATIRKHMALNLLLVQDQKDILVDKVSLDTLQLLIDNNHGCTFKVTKFPPLDIQKAYFVIKHIDQCTIESWDAINAVKLGDTKILDFLNSKPYTWNTSAWIYSPTIEVFEFILRNFSIGGTKDADPLAQLGNLALVQHIYNNHKSFTFSWKALRSPPEVLRFLLENLKKDQKLPPFQKIFINDQGALFKCDLFNFPAPPALSHQQLMLLLEAYGEDELNFKEDSLLFLSSIANNQPEIVKFLIDHNHVDFNRLSRIPGRVADVFFSAIRHSQIFKLCLTIFKDHKWFKDSFRKVFKGDWALKIWQTIISSPAAYRLEFVQGLKDLDLFPHYVHTTFYQADYYNENPHQPLTTTLVQSMLVDVQFKNLDFLDKLFVYAVKHGNYQLTKLMITVIEHWKAKLGIQQYHIESSFTMLRDRIELVTCQNCVTAIIDYLPPITKYSQPMIATLSPQVLDKLLSEILAQGDFDMHFHLLSKAIEYARFLKKPNIQQLLSNLPADIQQRLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V742</accession>
    <name>A0A8J4V742_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">SNF7 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_005130</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000197">
        <property type="protein sequence ID" value="KAF2073544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V742"/>
    <dbReference type="OrthoDB" id="441172at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1060">
        <property type="entry name" value="ESAT-6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF5">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="195"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="80"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2073544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="25806" checksum="A11D3A13CC9D8B0E" modified="2022-05-25" version="1">MGLLFSHCAGGGRQKNNKISNTDKAVLDLKIQRDKLKQLQTQINLVILRETAIAKECAKSNKKPQALLALKKKKYQEKMLEDSLQNLSNVEELINNIEQSEIQVQIFESLKKGNNALKDIQKELSLEDVEKLMEETEEAIQYQNDISEALSGKFSQDEEDALLQELDDMEKQMLQLPDVPKDKIDVPIEHIIQAPGKLIETQEAEREREREKQREREREPQMAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8H0</accession>
    <name>A0A8J4V8H0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_003654</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Endomembrane system</location>
            <topology evidence="12">Single-pass type IV membrane protein</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Nucleus membrane</location>
            <topology evidence="13">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000117">
        <property type="protein sequence ID" value="KAF2075044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="21607at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000099">
        <property type="entry name" value="Nuclear anchorage protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="15">
        <location>
            <begin position="1350"/>
            <end position="1456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="8"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="72"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="263"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="308"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="391"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="432"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="799"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="987"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1167"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="14"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="313"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="360"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="395"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="463"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="521"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1167"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="1200"/>
            <end position="1222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1242"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1321"/>
            <end position="1334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2075044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1460" mass="167565" checksum="158B28AB783E2B87" modified="2022-05-25" version="1">MFSNLLKWFAPADQPQNQQQQQQSNSNNNNSIYQQEEGLVHYHKVQNEALILTSQLPVPELQDHHKHNVDPLMGEMMSTPSSVPKSSNNVTTSTTEASVPSVDKELENKQQQQQKRNTLRLSNEDLPVVTETWEIDSDEDEYIKQNSISFQGFVSQLKSSKIKDDRHLTNIYQKYEFEKGEDLFKDDQDDGSAEILKSTAANNNNAAKTKRKSIHKKAGGLFDDTEDNDINAFLDQEKSKTRPKKQVVTVSLFEDDVNNLLEKQVFDSQQDDRPSSPTSSRSADLLKQQSDFDLSSFLTSDFDFKKKTPLSKNANNGNAPTTQPTTAPASTSWTNPNDKNVLGTTPTAQPKPAPKTKLSSQRERQEQPKKVNALEVDIFGDGVSNDDGFQFTLPTSTSNSTRSNNIHIGSRKDQQQQLSPIPQFNDDVVVAKPAVEQPKQSKQQSKHEYEDEDDIESIIKQVDNFKKEAKSKSDQDNDKLQQILFEQEQEDLKKKNTKPKPKVDTKDIDDLFNFGSKKEAPVTASPTPAAATATTTTTPAKSKAIDFTADIFQNDDQDDMFESIEKKYKKKEEPTTKANDVLSFLNWEKEESDRVEKQRAEQELILSNQRKEAEQKRLAEEAEQKRIADELERKRLAHEAEQKRIADELERKRLAEEAEQKRIADELERKRLADEAERKRVADELAEKNRLEEVERKRVADELAEKKRLQEEAERKRIAEELAEKKRLEDEQEKKRIADELATQKLLAEKEAERKRIADEKAKQEEAERLRLAEIETKRLADEAEKKRIAEQQERERLAEIKRLEEEAAEKERKRLADEAEKKRLADEKARQEEEERKRLADQEEAERKRLAEIKRLEEEAEKKRLEDKRIADELARQQEEERKRLEKETEIKRLLEEQEKKRLADEAEKKRLADEKARQEEAEKLRLAEIETKRLADEAEKKRIEEEQERERLAEIKRLADEAERKRIAEEQEKIRLAEIKRLEEEAAEKERKRLADEAEKKRLADEKARQEEEERKRLEKEAQELETKRIAEQEATKKRLQEEEQEKKRLADEAERMRLAEEAERKRLAEIKRIEEQEAEKKRLAEEKRIADELARQQEEERKRLEKEAEIKRLAEEKRIAEEQEQEKARKKLADELAEKNRIAQEQAEIKRLAQEKVIADQLAEKNRLANEKESTTSIDEADSSNHQDLIHLTIGRTKVKGRKKASRRALGKDGKRKKQILPNEHELKPSSNPMPDEGSSSVSESSTSDSPTTTSPTPTIKKPIGGIGMPGMMMPKFDPSAVKLKPSAGVKAATPSTAPSTPPPSNNFTFGLKPAGANSFNRPSGVTTSPSASPPTLKKAPAKDGAQKALDILLTWCKESTAGYDGVNVKDFTHSWNDGLAYCALVHHYRPKAIDMNTVSKTDRVSNYKLAFALAEKHLGVPQILDPQDLADLPYPERLSNITYLSEFFKTIKKNGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V9A5</accession>
    <name>A0A8J4V9A5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-dependent protein kinase catalytic subunit</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">CYY_002790</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000081">
        <property type="protein sequence ID" value="KAF2075899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="431717at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004677">
        <property type="term" value="F:DNA-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05172">
        <property type="entry name" value="PIKKc_DNA-PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000041">
        <property type="entry name" value="DNA-dependent protein kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000013">
        <property type="entry name" value="Protein kinase, DNA-activated, catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037706">
        <property type="entry name" value="DNA-PK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046804">
        <property type="entry name" value="DNA-PKcs_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046803">
        <property type="entry name" value="DNAPKcs_CC1-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012582">
        <property type="entry name" value="DNAPKcs_CC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045581">
        <property type="entry name" value="DNAPKcs_CC5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003151">
        <property type="entry name" value="PIK-rel_kinase_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF68">
        <property type="entry name" value="DNA-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20500">
        <property type="entry name" value="DNA-PKcs_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20502">
        <property type="entry name" value="DNAPKcs_CC1-2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08163">
        <property type="entry name" value="DNAPKcs_CC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19704">
        <property type="entry name" value="DNAPKcs_CC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02259">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01344">
        <property type="entry name" value="NUC194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAT" evidence="16">
        <location>
            <begin position="2922"/>
            <end position="3569"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="15">
        <location>
            <begin position="3749"/>
            <end position="4088"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="17">
        <location>
            <begin position="4129"/>
            <end position="4161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1616"/>
            <end position="1638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="2730"/>
            <end position="2768"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="614"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="2733"/>
            <end position="2757"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF2075899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="4161" mass="479197" checksum="962A7D7510A5F430" modified="2022-05-25" version="1">[encoded data, 4161 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VAP4</accession>
    <name>A0A8J4VAP4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C-CAP/cofactor C-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001297</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TBCC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000030">
        <property type="protein sequence ID" value="KAF2077369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VAP4"/>
    <dbReference type="OrthoDB" id="427777at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051684">
        <property type="term" value="P:maintenance of Golgi location"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017901">
        <property type="entry name" value="C-CAP_CF_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016098">
        <property type="entry name" value="CAP/MinC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039589">
        <property type="entry name" value="TBCC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012945">
        <property type="entry name" value="Tubulin-bd_cofactor_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16052">
        <property type="entry name" value="TBCC DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16052:SF0">
        <property type="entry name" value="TBCC DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07986">
        <property type="entry name" value="TBCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51329">
        <property type="entry name" value="C_CAP_COFACTOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-CAP/cofactor C-like" evidence="3">
        <location>
            <begin position="179"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="137"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="337"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="85"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="153"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="481" mass="53015" checksum="924FF093146BA7FF" modified="2022-05-25" version="1">MIENKVSNKNNINNNSNINSNNNSHNSGKLSGINNKSTSNASPPTITAAISNSSVPINNSNNNNNNSPLSSPQHILTGPMKSQINNNNNNNTNNNSNNTSYSTCNISQIFQKTILRTEQDFIGQSLENYLQIHAGEHNNNRHSPRFNINKDASPSSSPNTTTTTTTTTTTTNSPSTLAPLNSNNNNNNLSLSPKINSENGNNNNNAHLSIKVSLGQIKKSFIYILVNMSFCTISNVHDSTITLGPCTDFIEVSECSRLTIISITKGIRIKSSNNISLFLCTNQKPIITSDCSDIKLAPYNTHYPNLELQINQSKLSTQLLDNQWNNPLIFKSFLPQSESSTNTNTNTATTTTTTNSTTNSLTNSNDLNSSSGSLSSSTGESPSFSNIYKILDPDEFYTFVVPFLIKGKTKSNPCELPSKYLKSLVTKSNAVHRLHKSIQQTNVDNKTKAHLLYLIESKFQEWLKETDNIRQINDLINLKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJF8</accession>
    <name>A0A8J4PJF8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ABC-2 type transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_010040</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000897">
        <property type="protein sequence ID" value="KAF2068635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJF8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF265">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="163"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="202"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="ABC-2 type transporter transmembrane" evidence="6">
        <location>
            <begin position="25"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2068635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="279" mass="31951" checksum="59FCD5512C640714" modified="2022-05-25" version="1">MEDVASDLHLDFKNQLTNDPKYINDSTYFVVISRQNNSAFDIFIEYDITASTSLLWDMLFYRVYEEKITTYLTLIQTKIEKQIMKTFYSKDVDIQITYQIPPKITDLKYEPDSNLINFPRWIPSFFFIGFTIPIVIFIYQIVLENEKGMRNQLRILGLFDGAYILSWLTDGLIVSFIKSIIVLIFIYSNNSFTALSYAPPSILFVIFITYGFSLYSFGIFLSSFLSNSKSAVGLGLGLIIIGGATSISFGFIGSDIFRIYSYDKLIPIGILISLVPFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PK38</accession>
    <name>A0A8J4PK38_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 3 subunit G</fullName>
            <shortName evidence="5">eIF3g</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Eukaryotic translation initiation factor 3 RNA-binding subunit</fullName>
            <shortName evidence="5">eIF-3 RNA-binding subunit</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Eukaryotic translation initiation factor 3 subunit 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_009500</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. This subunit can bind 18S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the eIF-3 subunit G family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000725">
        <property type="protein sequence ID" value="KAF2069177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PK38"/>
    <dbReference type="OrthoDB" id="1749473at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12408">
        <property type="entry name" value="RRM_eIF3G_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03006">
        <property type="entry name" value="eIF3g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017334">
        <property type="entry name" value="eIF3_g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024675">
        <property type="entry name" value="eIF3g_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034240">
        <property type="entry name" value="eIF3G_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10352">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12353">
        <property type="entry name" value="eIF3g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037949">
        <property type="entry name" value="Transl_init_eIF-3_RNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="163"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2069177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="244" mass="27487" checksum="C768A4974D4DE133" modified="2022-05-25" version="1">MSSVEQNEWSNEAPQTVEKVSEVIVKNEKGEEIKITKRYQEYQVTVKRNRKADERKKWKKFGECADKTGLENTSYGDEQFLILSRKTESKEEEKDIVKCRICKKNHFTAKCPYKEALDLSQKPQRATAGGADGGEKKETGGSKYVLPSQRAGHVAAPSGNDIPSLMVSNLSDNANEADLRELFGSIGHVYKINIPKTLDGRPKGFAYVTYANLQDAEQAIKTLNGHRYDYLVLGVEFAKRKSQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKC4</accession>
    <name>A0A8J4PKC4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RuvB-like helicase</fullName>
            <ecNumber evidence="12">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_009924</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the RuvB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="12"/>
    <dbReference type="EMBL" id="AJWJ01000855">
        <property type="protein sequence ID" value="KAF2068754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKC4"/>
    <dbReference type="OrthoDB" id="10060499at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002221">
        <property type="entry name" value="RuvB-like 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000010">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.360:FF:000002">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.360">
        <property type="entry name" value="RuvB-like helicase, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027238">
        <property type="entry name" value="RuvB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041048">
        <property type="entry name" value="RuvB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042487">
        <property type="entry name" value="RuvBL1/2_DNA/RNA_bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010339">
        <property type="entry name" value="TIP49_P-loop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11093">
        <property type="entry name" value="RUVB-RELATED REPTIN AND PONTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06068">
        <property type="entry name" value="TIP49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17856">
        <property type="entry name" value="TIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 12" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="67"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2068754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="457" mass="50299" checksum="A3435FD28EA7C887" modified="2022-05-25" version="1">MSSIVPKISEVKDLTRIERIGAHSHIRGLGLDDSFEPREVSQGMVGQVNARKAAGLILQMIKEGKIAGRAVLIGGEPGTGKTAIAMGIAQSLGSNTPFTAIAASEIFSLEMSKTEALTQAFRKSIGVKIKEETEIIQGEVVEIQIDRPATGSGVKVGKLTLKTTSMDAIYDLGSKMIDSLTKEKVQNGDIIKIDKGTGKITKLGRSISRVRDNDITGSKVNFIECPSGEIQQRKPEVHTVSLHEIDVINSRAQGFFALFAGDIGEIKAEVREQINHKVAEWKEEGKAEIVPGVLFIDEVHMLDIECFSFLNRALEDEMAPILIMATNRGNTTIRGTDYKAPHGIPLDLLDRLLIISTTPYTEKDIQKILKIRCEEEDVDIKEDALALLTKIGVETSLRYSIHLITSASLVCVKRKGTEVSVEDIKKVYDLFVDVKRSVKYLKDYQDEFLHNTPITNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKP8</accession>
    <name>A0A8J4PKP8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Attractin/MKLN-like beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_009031</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000621">
        <property type="protein sequence ID" value="KAF2069655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKP8"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056737">
        <property type="entry name" value="Beta-prop_ATRN-MKLN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051746">
        <property type="entry name" value="Kelch_domain_containing_8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46260">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46260:SF3">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24981">
        <property type="entry name" value="Beta-prop_ATRN-LZTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Attractin/MKLN-like beta-propeller" evidence="5">
        <location>
            <begin position="365"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="95"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2069655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="602" mass="68266" checksum="D7F1E6251319E997" modified="2022-05-25" version="1">MIMNDVSTTPKVSITDTSSPSKDSHPNNQSNSNININNNSKSIIDTWGKTVNQLECYKELSEQTLEQCDESFIQCKKKIIQDFDTLINYLNDRKVEILTQLAIELESHKQELETNRDRATILVDQLNRKINGLDNKSDHPHPLTHSNSMSNLSGVLSPLKKSTSFQTDHLQVLKESISVIEWDWCGEFQSQTTTTKIIKSPKPVSTFVCNLLNIHEEEGNGNGGSVYNDDLESKTSISIEDSNGGGGGGSSITTSSSSLLSYLSKQNNGGVGNEKEVNVFGKLEFKVVENPEYKKLNSELPEKKFIFSIGGKLNGFDQYAMEKYDFKENIWRSLEPIASMDNDFTCNYDGKNHIYLFGGSLSPTRAIKYNILENQWEKLFSTCDNEIPEGGRFLHSSVFDQKQFIYLIGGFPRSTCILKFNLFTEQFSRQSSQTKSLWNISTIYESKSNSIYLVGGCNISRQSVNNFERYDVEQDKFYALEPLPMALYSTGSVLDEKNGHIYVFGGFNSQSNQCVSHVYRYDIKHNQWQKLDAEIPKQITISNSCLYDNDQFIYIVGGYHCVTKEFTENVYRFNIKSLEFDTTIPPYLHSKLKGGCSLFISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLG4</accession>
    <name>A0A8J4PLG4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_009629</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000761">
        <property type="protein sequence ID" value="KAF2069050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLG4"/>
    <dbReference type="OrthoDB" id="25146at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46115">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="7"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2069050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="102" mass="11346" checksum="425D19F4285782B9" modified="2022-05-25" version="1">MSIIEIKSASQFDEELSTNELVIIYYTAKWCRAIGPAVRVLGEDNPSVIFLKVDIDLVSDHPLVKSVTSLPHFVSRKNGVEIEEFTGASPDNLKKMIAELVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLJ2</accession>
    <name>A0A8J4PLJ2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010312</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001033">
        <property type="protein sequence ID" value="KAF2068361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLJ2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053097">
        <property type="entry name" value="Prespore_vesicle_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34586:SF8">
        <property type="entry name" value="NUCLEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34586">
        <property type="entry name" value="SPERACT/SCAVENGER RECEPTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="219" mass="25515" checksum="D7821DABBD2AB4F6" modified="2022-05-25" version="1" fragment="single">MDIVENSNNTSNSEDDNDDLHSGGDEKLILKKLDKYIRNNRYMKIYKYIFIDIEHDLHIWDRLFDVWLIKIFGTTSNNDKLDCELFDKQLMVSFISTTNNTSSPSTTTTTATTTVKKSFLSPPILENSLTKSLHEVIKSYSMDFIINDYKTLQRYFHKYFIIDLPNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLN7</accession>
    <name>A0A8J4PLN7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009508</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000727">
        <property type="protein sequence ID" value="KAF2069170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLN7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="137" mass="15888" checksum="4EDB1D57D21D02D1" modified="2022-05-25" version="1" fragment="single">METERNADKEFNNDQDDEEIEIEIEIDSNSTHPLKKRKIDQNDGDQDPLVTKQDEYNEDEEEYEPLPSSLKILIPSSIQSVSSNLDYKSYRSLVTVLDKDDYYNYSTTPTPPKIRQGLKELVLPKSFNNHIHNSTLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLP9</accession>
    <name>A0A8J4PLP9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009405</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000700">
        <property type="protein sequence ID" value="KAF2069278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLP9"/>
    <dbReference type="OrthoDB" id="23058at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550:SF11">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140860">
        <property type="entry name" value="Pseudo ankyrin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="652" mass="76346" checksum="7F23D2456608276B" modified="2022-05-25" version="1">MNLLFNQVYFNSFIKKQILDHLVHSINYKTINYKYCFDVAWLIRNENYGLLASKLHHHEYLYLDKYRIKDLFKIKDKKLFIQCFEKFRVYYDNHGGIVCGHYQDTEANVNNVQSSVLEFAVEHDNDDAFMYLFDRQYQFTGSLLLELLCTKGKLDLIRYIATSMKEIPNQYVSVECFLKAVKCQDKELVALLFQLFGEFFSSQSLENREKILIESLDYGGLEIFNLVQKYFQESIFLFSLRKGLIYNKTMLWTLYLCTLKSYKTFTYLLDHFDLSFTQIEQEPDFFISPHIVASSFGDSLVVKHMLETNKSLQQDIDPMCFNALVEAHFEMYSMIKTHYNSPLVPLPRFFKIGCMKEKSLTCENVKYLVETLKADISREDLERSGPFEVFKYLFLHHQNIKSGLESNGLNDYTFVNSVIDRAYKQSNIDYIVLLHKQGVSLKENHVWSRDLELFGNLDKRVAQHFLKTLISICPPSVDDLETLVKALEYFCRHSDNVNIIKLLYGQVIAISGDSDTDTRPSLSHAAQGGRFQTLLFLFNKNLKPNNYYELLEAAARGGSITVMKYIFEKYAFHLSNIQSHPKILENAIMYNHLNCVEYLVPLYPKLDNLSYQVLRNINDTGNLMMAKFLFESMRFNKKIITVLNLCLQKFDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLX2</accession>
    <name>A0A8J4PLX2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">IST1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008275</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IST1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000496">
        <property type="protein sequence ID" value="KAF2070405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLX2"/>
    <dbReference type="OrthoDB" id="29853at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.60:FF:000002">
        <property type="entry name" value="Vacuolar protein sorting-associated protein IST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.60">
        <property type="entry name" value="Vacuolar protein sorting-associated protein Ist1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005061">
        <property type="entry name" value="Ist1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042277">
        <property type="entry name" value="IST1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12161">
        <property type="entry name" value="IST1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12161:SF5">
        <property type="entry name" value="IST1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03398">
        <property type="entry name" value="Ist1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="232"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="235"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="248"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="277"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="288"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="321"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="378" mass="42745" checksum="2FDF09BC2BAB3257" modified="2022-05-25" version="1">MFGPQFNQSKLKVQLKLAVSRIQIQRNKKLNLIRDEKRHIAELLRNKNDESARIRVEAVIRDEYLVDCYNIIEILCDLTFNRLQLLVEACEIPYEMRESIHTLVYAAQRLQVPELHLVSQHLKAKFGKQINQENSQCCNGFVNPNIVSKLSMVVPEPFVIFQVLSCIAQEFKIDWCPGVEPITLQAPAMRVEKSMMHPHQQQPIMQQQQPIIQQQQPIMQPMQPIINVQHQSPMMTPPAMPSPPPFPTIIAPQQPPAYQQQPPPSYQPFPNINVNPSAPPPAFPTPPSSLHSSSSSAYSNNSYSGNSNNNMNNNNFNNSNGFPSIPTTSNTNKDTYQNFTSFSPAPYNPTTTTGDTTASIDFDMLTARFEALKKQNGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM50</accession>
    <name>A0A8J4PM50_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009198</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000653">
        <property type="protein sequence ID" value="KAF2069488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM50"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="408" mass="46922" checksum="8242DE4AA13F5469" modified="2022-05-25" version="1">MDMDSYSFNLLIDNPFIDDIFEFTKRICRASDNASKEIDVFVVELLIHKVRLLAISYKNGCSGPYGSFANDLVLACWFSPEIHEYLSTFLVGNNVMIGDRDSIVLDEMAMYGFQVMCFMKAVFEFDLEKIKSITSSWSTRLLRITYSTFAFDTFYASLACAKTFQQKRDAIVVGEYLKSQFRQLELRNAEFSSALVYFQDKAFYTEISRAPLENLSNIINGVMMGGGSFGQLKYLVENLTEIEYNGIPVEDVIHSFAVHSRGTFLKPILYLFEALQDRFKSESILDHLVCTAVEYDNHRLINILLHRTKIRLIKPKSEQHEQQILSRMKLVLKSALPHVESSTYFQHHADFEIDQPLLVDLFFGRMPISYSSTKVDPNVLIKTIVPEHSELIDQSVSFARRKKAQNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM87</accession>
    <name>A0A8J4PM87_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA sliding clamp PCNA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_009092</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processivity during elongation of the leading strand.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the PCNA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000635">
        <property type="protein sequence ID" value="KAF2069588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM87"/>
    <dbReference type="OrthoDB" id="534348at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043626">
        <property type="term" value="C:PCNA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019985">
        <property type="term" value="P:translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00577">
        <property type="entry name" value="PCNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.150.10:FF:000006">
        <property type="entry name" value="Proliferating cell nuclear antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.150.10:FF:000008">
        <property type="entry name" value="Proliferating cell nuclear antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.70.10.10:FF:000001">
        <property type="entry name" value="Proliferating cell nuclear antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00317">
        <property type="entry name" value="DNApol_clamp_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000730">
        <property type="entry name" value="Pr_cel_nuc_antig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022649">
        <property type="entry name" value="Pr_cel_nuc_antig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022648">
        <property type="entry name" value="Pr_cel_nuc_antig_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00590">
        <property type="entry name" value="pcna"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352:SF0">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02747">
        <property type="entry name" value="PCNA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00705">
        <property type="entry name" value="PCNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00339">
        <property type="entry name" value="PCNACYCLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA N-terminal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA C-terminal" evidence="9">
        <location>
            <begin position="128"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2069588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="255" mass="28155" checksum="AEA792E975BDE8B2" modified="2022-05-25" version="1">MFEARLLKASLLKKILEPIKDLVDSANFDCSPVGISLQAMDSTHVTLITLLLRSDGFDSYNCDRSLSLGLSLVSLNKILKCAGNDDFLTIKARDEADTVTFVFESPKSDRISDFEVKLLELKNDQYNIRDSDYSAVVKMPSAELQRICRDLSIMGENVTITANKEGVKFSVSGDSGSGNITVRPTTDTEADLATTIESKEPVVLSFALKFLSNFTKATPLSPTVKLTISETDPIVIEYKIDDIGYLRFFLAPKMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM95</accession>
    <name>A0A8J4PM95_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SURF1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_008970</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Probably involved in the biogenesis of the COX complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Mitochondrion inner membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SURF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000609">
        <property type="protein sequence ID" value="KAF2069714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM95"/>
    <dbReference type="OrthoDB" id="10040024at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06662">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002994">
        <property type="entry name" value="Surf1/Shy1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045214">
        <property type="entry name" value="Surf1/Surf4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23427">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23427:SF2">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02104">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50895">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2069714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="261" mass="30280" checksum="1EE5C8D38F5E1B0C" modified="2022-05-25" version="1">MQQSKRSFKVFIIFPIISAGLGTWQVYRYFWKKELIENAVAKTELDPIQLNDNVDNLLHNNQPFEFRRISVQGQLDPNKEIRLGPRGDGNGIIGYYTISPLTLDDGKTILINRGWKPNPAKTNYRNPTLISNDATTTSENKQPITDVNVLGLVSKKKERGSMFTPENKPMYNQWYYLDVEEMSKVYNTLPIMINAIEEDETIDNGKKKTTKKLQDNSQIKRFDTDVESNFHNKHMSYIGTWYSLSGCLTFIYFKFMRVPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMV1</accession>
    <name>A0A8J4PMV1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">inorganic diphosphatase</fullName>
            <ecNumber evidence="3">3.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_008835</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000586">
        <property type="protein sequence ID" value="KAF2069845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMV1"/>
    <dbReference type="OrthoDB" id="1608002at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00412">
        <property type="entry name" value="pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="INORGANIC PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50324">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00387">
        <property type="entry name" value="PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2069845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="256" mass="28514" checksum="C0E053D88A473B12" modified="2022-05-25" version="1">MTYTYKQVNAVGTLESRVYTLENGNVISPFHDVPLWVKPGDVANMLVEIPRGTNAKLEIATKEFMNPIKQDVKDGKLRFVHDKYPFNYGALPQTWESPATVHPETQCKGDNDPIDACEIGSAVGVTGEFKQVKVLGVWAMIDAGETDWKILCIDVNDPAASKINTIEDVAQHLPGEIEKVFTFLRDYKIPDGKGPNKFAYDSKLLDKAFAVNIINETHHDWKTLFTTENPLSSVNTSVKDSKTCISHEDVKAKLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN83</accession>
    <name>A0A8J4PN83_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein FRA10AC1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000491">
        <property type="protein sequence ID" value="KAF2070443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN83"/>
    <dbReference type="OrthoDB" id="197967at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019129">
        <property type="entry name" value="Folate-sensitive_fs_Fra10Ac1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09725">
        <property type="entry name" value="Fra10Ac1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="139"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="275" mass="32205" checksum="275C3FBBEA7DC5B8" modified="2022-05-25" version="1">MESKYINDLQNLDAITRHNRFIQSYLKNSDKNSNHENKSNQNNNNLDKYKNFKSDYQILKENYKFIRDDDDDNVDVDQLTWEDKLAIKYYNRLYKEYAIIDLSRYKTGEIGLRWRVKSEVISGRGQFTCANRKCENVPSSTPSSSSATTTTTTTTTTQSINNNNNNNKIELKSYEVPFSYLEDNQEKTALVKKLKQILKEQKKEKKKEKKRKYSDNSDSDDSDSNNDDNSSIKEKKRKEDDQGNNTDTDTDTDTKSKSKTYSKQEFDDFFEGLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNP5</accession>
    <name>A0A8J4PNP5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007902</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000446">
        <property type="protein sequence ID" value="KAF2070788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNP5"/>
    <dbReference type="OrthoDB" id="20899at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14686">
        <property type="entry name" value="bZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14312:SF1">
        <property type="entry name" value="BASIC-LEUCINE ZIPPER TRANSCRIPTION FACTOR A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14312">
        <property type="entry name" value="CREB/ATF BZIP TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="2">
        <location>
            <begin position="348"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="550"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="809"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="948"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="333"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="559"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="588"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="600"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="809"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="831"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="883"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="948"/>
            <end position="961"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="969" mass="110951" checksum="D5B2531FC4608F9B" modified="2022-05-25" version="1">MESDSNIFNQGNPYFQDYNMGNPALGNSASSLFFNKSLNTTDFFNSSNNNNLFLNNSNSFGQILTPQQLLQQQQLQQSQNFFTNDFQQQQQQQQQQQQQQQQQQQQQQQQLKKSNGNNNNNNNNAFNSMNVLQNQNKELNIQHLVQQQQESFKKRLIDQGVALPDSELNALFFQEQQRYLKQFSEQINTNDVAAPENNVVHIQQQVPQHIQSSGVVDSFPIVQGIPQDYLQQQQQILQQQQILQQQQQQQKLLEQQQQQQQKLIEQQQQQKLLEQQQKKQQQHHHHHQQQQQQQLSTQQVLQQQKLLQLQLQQQQQHEDHKKHSKSRSKDNNYDDDDDDDFCDDDDRDKSKSKSRSSQNIASRNYRQRKKDHIHEVEMKVQQLTMENERLRSENVLLKKGDLGDVMRPDTDFQQVLLESQKLMSQLHDAVCKQDHASIEYLLQLYYFASQLRTTVVEKEVEKIVHPYTQARLAIMGYQSCAETSLLLKPFSTNLWWPKFSEEVGLSEEQRQASDILWADHLKIDQELRSERDRLDREIKEIFLKKIVSCGDLTNNGPNPDDITTPSTSNDTPSSPYSCSSSMSSPSTSSPPPPHSPPDSPINHLINSNNNNNNNNNNNNNNNLLISPTTSSSTPTSTSSSHNPYPVPNNVNLQEMAHHRALFGDGHNIELSELLDLTRRLELLKKNFVKHRNLICDTDLVLSTILTPFQHAKLILRLNSVTCYDISMVDTITTIWGTIHTAVKDGKTFISNLMPNEDGKFEVLEKFKKEKQKVNFKSEQPFKLESLQKTYEKLYNNVFSVSTNAPITQPFFQPTDQSKHSNLPTPSSMNISPNTSSPLQQPSSPSQQIPSSPFLDLSQQQQKSNSSNNGSYSSLPISPIPSPNIQNLHMSGNSKDSSSAENSPAQSTSSTSSSGKKKAKKFQWYSYKTPGVLANIDSNAQIEMVIREKPEGKDANHPDNIKNKINNSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNX1</accession>
    <name>A0A8J4PNX1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008716</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000565">
        <property type="protein sequence ID" value="KAF2069966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNX1"/>
    <dbReference type="OrthoDB" id="25620at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="532" mass="61598" checksum="DA7B9D14DF09CC7D" modified="2022-05-25" version="1">MAVDQTHYKELNHFKVNISPSLIDKMKKEKRVIILIESTKHMDPYVSMIKEIVKYLFNTLENQTIHIITFDDDYKLVEGLDTPDYFKALDQIQCFMDKENSMEGFNKALVKVLEMTMNEKSSHLIVFGKGNWVAQNEQDFENPVFESILNNALSLEKNNQFVTFLLGKDTNQKEIRDLFSKSYFNIYKIESDFYQEIKRLFNQSSCPAYVQATPADCIIRDISLKLKIEMFPRNNLEIFNNVTFKHAHESGSISQETSSHDVTFTEPFTVSIDTGLKAPKRISFSIIINGQEFNGEIEPHISWFLRNIKFSQLVYTIAFAGTRGVGKSSSINSLLNLFSCQNQIFEKNVAGFLNDHVTLEINKTKVSQVLDWFDHPLVEQLYQLIGSMIIYDIPGHEVGRSRVDVQSIQIEQLVDQFKPDTILIPFSLQNEDELDIKYINSIVELLRERDISPILILTFADNLSPTDLSNRKSFLMHKLNFDSKDVFQLINYAEETVRNFDKDVSIFNIFSRAIENSQICKNKKRINNKNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP84</accession>
    <name>A0A8J4PP84_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ras GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_007804</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000437">
        <property type="protein sequence ID" value="KAF2070868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP84"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00876">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002309">
        <property type="entry name" value="Ras-related protein Ral-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="191" mass="21851" checksum="19F7F8ACA56C7414" modified="2022-05-25" version="1">MFNFKLVLVGPGGVGKSCLTIQFIAQKFVDEYDPTLEDSYRKQTTVDNEECLLDIYDTAGQEDFSAVRDQYMRTGEGFLCVYSITYLQSFKEIPRLHNHLLKVKDLDSVPFVLVGNKCDLNEYREVSTAEGEELAKKLKCKFLETSAKERINVTEAFYELVREVKKGRNNNQPVAEQVEAPQKKKKSCTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPV5</accession>
    <name>A0A8J4PPV5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007268</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000377">
        <property type="protein sequence ID" value="KAF2071424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPV5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="288"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="321" mass="34419" checksum="7A072AC939D691B9" modified="2022-05-25" version="1" fragment="single">SNSNSNSNNNNSHNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNTNNNSSNTNGNNSPVNSNSSPNANSINIINNSTTNKNNNNSSNNNTNTNTNSNSNSPTTSSGTASNLNINNNNNNNNNNNNSSNPSSSTSNNNNSSNSSSSTPPISPVNISNSPSISPALVPIVSDDLNKPKFQKKSLSVVIPESGASKYAVPLTKLPAPMINESGAPMVSNKETPTLPSPREFYPDIGTDGISLTPSFWSWNTPRGFTTPSSLQNLETLPLTGALSPNKNFSFMDAYHSNQLNNNNNSNSNSEENGSEYRKRKTVSSKHQEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ70</accession>
    <name>A0A8J4PQ70_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006686</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000319">
        <property type="protein sequence ID" value="KAF2071997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ70"/>
    <dbReference type="OrthoDB" id="20621at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="343"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="382"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="498" mass="53704" checksum="181DFAAFC7956235" modified="2022-05-25" version="1">MTISGIVIQQHDGTILFSNVREYLSPDKALLAAKAFKNHISLNDDHFITKGSTNHLTGSIVVLNMYRVHYMILNDIFILAISQANDNPYEGSVYLVRVKRVLWTVSKELLIHNIMKKYTEVYFALERVLFGEDGVDVLTQRINEIQPHFTQSTQPTTQNLIAPPSFITGSQNTPQPVNIFGRIIETTNSNNVIPPSLTYSNSSSSTPISTTPTSTSPTSNIINNSFSSPSNNSNSSNNLSSSQVSISSTTSSLSSNNLLNSSSSNGFIIDNNLDYQQHQLNIISSLENIEIPAKVFTQPDTLPKIDKHIYDPPQTVKWGNTSVQSTATTSTRVTNRIMFTLRHPNAAQNSKDENEGSSNGSGGGGSVAMNHSSGGAGNSASKDTTPISSPLASSFNSLIDVSSPDFNSSDDGKQQSSPENVAQSSSPPSNGDELPPTEPKRKFSLSMGTFKNNSTNGAQVANVCSKPDLETSSAPQPTPPPSVPIPANVKSKMNFLNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ83</accession>
    <name>A0A8J4PQ83_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_006970</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000347">
        <property type="protein sequence ID" value="KAF2071709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ83"/>
    <dbReference type="OrthoDB" id="19242at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050940">
        <property type="entry name" value="Actin_reg-Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46485:SF5">
        <property type="entry name" value="CENTER DIVIDER, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46485">
        <property type="entry name" value="LIM DOMAIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="276"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="63"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="151"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="164"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="211"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2071709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="542" mass="59466" checksum="AAD2283F65C49702" modified="2022-05-25" version="1">MIVGAHVRELSTGSLGVIEKGNNANGFYVSFGARKNWKRYNEIEVIDSSSIGGAKAKATIPAHSATGGAGETSTPGNRPPPTPISASSSTSGGNLKKVSPPPTINNKTFVPKVQSEDPGPRFGNFKFNTTQNTNTNPKYSTLPSRAFFDVSSASSPNGNNFKKTSPPGSSTTSSPSRPAIPDFSNPPSTKNTQSTTTTNVVSNQNGHIVNPPKPQPKPNTPQPTPQPPTQTPISKPLPQPKPNPVPPVAAISKPPPPSIVSPRKNKDLWNIDFSELTFDNVIGNGRYGEVSLGQWLGTPVAIKKLMENDEEANGLVDREIEILKEIRHPQIVQFLGVSRNKENETHIITEFMDGGDLFDALIFGDFELTWKEKLRISLDIAQACRYLQRQGILHRDLKSQNVLLGSNKRAKLCDLGLARVFDDRINKRLTSNLGSDAWMAPEMFMGEEYDYKVDVFSYGIVLVEIITNAVPDPRDPMKRFAFNSQNFMKKVPSECPPAFAKLTVDCTLTNPKERPSFTQILETVKSIYDSLPDDCYDDQEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQX2</accession>
    <name>A0A8J4PQX2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006192</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the csb family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000275">
        <property type="protein sequence ID" value="KAF2072483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQX2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008601">
        <property type="entry name" value="Dicty_CAD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05720">
        <property type="entry name" value="Dicty_CAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="110" mass="12519" checksum="385F45552A5B3906" modified="2022-05-25" version="1" fragment="single">MSQQGFPISVSIDSNGKHTIKGNGCVLPIPKVFPNPCFLMICGPAKFEGDSEVWKGRKEVKNVILEYKGKSYRLGDCRYDAINEDFNEDGINDYRINLWPKTPVQDIPYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR76</accession>
    <name>A0A8J4PR76_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006114</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000268">
        <property type="protein sequence ID" value="KAF2072565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR76"/>
    <dbReference type="OrthoDB" id="5421at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051228">
        <property type="term" value="P:mitotic spindle disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001440">
        <property type="entry name" value="ATPase, AAA family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003783">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase TER94-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF194">
        <property type="entry name" value="ATPASE FAMILY GENE 2 PROTEIN HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="1">
        <location>
            <begin position="297"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="1">
        <location>
            <begin position="572"/>
            <end position="709"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="792" mass="88948" checksum="2738CD502AD4E990" modified="2022-05-25" version="1">MVINNNSSGGSGSGDKLILYPLDSSLIDNNIQIDQQSRYVNENCYISVHLFDKYHLRLGDWVFLEIHSNSSNNNNNNSNQCLISKVWTSRYIQQDQYIQANQSIILFRNNNSFDENIYSNLVSIHKINQYDSKLNEIDIKIIIQDTSDNNDKKNGNHSSLNEYKSLLYKKQFIKELLLYKMVAKGCFVQTNYKDIRIHIVNINNSENSIGIVTTSTKINIIDDNSESGGNNNDNRQQETSSKYLAFKSNLLDIYTINKTQGIQNSIGGMNDKIISLEEMIIYPILFPLVFEKLSISPPKGILLKGPPGTGKTHLVRSFCDSYQIKMFSIDCTKISGVYLGETEENLRNAFQEISDYSVANDNIPAVLFIDEIDTICPQRSKSNQNESRVVGQFLTLLDGMDGRKGNFVVIAATNRPNQIDPAMRRPGRLDREIEIPVPTKEQRLQILSVYAKSLPISSDPPNLLEIVAEETVGYVGANIQFLCRDASLIAFSRVKNSPADQSSATMFIELQDFRESIRHNPASILKGDSLVQVSNITWDDIGGLEDVKQQLKQAIEWPNLYKDSFARLGLSPPKGIILYGPPGCSKTTLVKAIANSSKLSFLSLSGASIFSPYLGDSEAAIREVFKKARQTIPSILFFDEIDAIVSKRNEGENKSTELGLLSTFLNEMDGVEQLNGVIVIGATNRIDMLDPALLRPGRFDKIIEISLPDQDTRLKILQVKTKKIPIANDVDLIKISEITRGYNGADIENLCREASFQCLRRNIENPGQVTMNDFLSILKPQERKQKDNISLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRG8</accession>
    <name>A0A8J4PRG8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_005741</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000237">
        <property type="protein sequence ID" value="KAF2072948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRG8"/>
    <dbReference type="OrthoDB" id="1436450at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23051:SF0">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23051">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35, MEMBER F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="328"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="419"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="297"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2072948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="465" mass="52842" checksum="3EFE702C4A743099" modified="2022-05-25" version="1">MNRGDRNNSYPDLNNDDDNGDNENIEIINNVDDNDEIENDIDNSAIIVGNYDDNTNQITNNNQHNNEDLSLINNDNNDINKSKPFQLRRHIIGTICILAVVFLWVFSGVMTQIIFTEEDYNKPFFLTYFATSIFTFYLLGFGIKWRTWLSIPFSRKQQKQQTSQIESYQSLNNSDNVNIESNQEQHSRTKVMDPVTGIVKNQYHFTIKEILKISLCLCPIWFCANYTYNLSLSRTSVSTNTILSTLSGLFSLFLSVAFKVDKFSIEKLLAALITLSGIIMVSYSSLDKSNNGNDTVLGDGLAVVGAFFYGLYGVLIKKLVISEEYLPMPMLLGFLGLFNFLFLWPLFIVFNLVSFEVFQLPSWRVFLFLVFNGLFGSFLSDLLDSYSVVMTSPMINSIGLSLSIPLAMISDFVRSHRSFGPLYIVGSIFVVFGFILANMASSVLEDRLKAIEQNLLKRFKKTLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRP6</accession>
    <name>A0A8J4PRP6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000331">
        <property type="protein sequence ID" value="KAF2071871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14596">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14596:SF72">
        <property type="entry name" value="ZINC FINGER PROTEIN MSN2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1474" mass="172167" checksum="B53403A100733D4D" modified="2022-05-25" version="1">MCDLCNYEDCNEYPHDRKKLLDCINLLNILDENIKKFMQDNTIKIQDNLSHFAQEIRNNEQIITRLRELKQHIKDRLNNHYLQSLETEFSILENRISSSINQLTETKQTINDYLNNFNTKKSATQLLVQVLDWFKEKNSVCSYKIYSSTTFQEYDIIDQLLNSYYQSQQPLTPTIFKENGFIHRLLNRFYKPQKHSLTPSDKQQQQFKNTLKQCIDIKESIDTRPLLCYPIINSYKFFNLPKHPIVEPIINSQLDLFLNSKTTPMKNNNINNKNDNNNYNVFHILNVPFNDRVTRLFYLISKSKFQFKYLFFVKILDDYKNILIKLDKNFSHSAIVGDRQLIDLFNSHVNNLTSVLIIYNVYFYDRNKYEIQYKNSNIKTIIISDSDFKADHPNVNIFGQHVLEPLSNEGLELLFNYLTDSIVSKDDIFYKPLMFIITQDDLKFMIKHCKDIRNKTKTPNDIIYSRLSNATIDRVPNEIKAENLVFKFPVGEMLCSFEVNYFKSQTKQNNEWVPITIMQSPNECTDPYQLILTSEYAQIIVNNNNNGDLYNIKFDLRFKKICYNLHYDGCINLIPLSGTFESRSQGIQSLSLSNCFGSILYRPTQDYNSFFIEIDAIGYSFSTNNNNNNNQNFEKRIAIRLVHGGRSISFINIEDTIHLIGGVKFINDTDHTLIITSRYSRFANNNQLIFKCKDNKPILDTGNLSGNLCGGVILKDDISYVCSTCNTPQCDLCNHEDCSGLETKGLFDYINVLDILDENIKKFMQDNTIKIQDNLGHFEQQIRNNKQIITRLRELKEYIKNRLDNHYLQSLETEFSILENRISSSINQLTETKQTINNYLNNFKTKKSTTQLLVQVLDWFKEKNSACSYKIYSSTTFKEYDIIDQLLNSYYQSQHSLTPKTTPPTDQQQQFKNTLKQCIEIKESIDTRPLICYPIINSYKFFNLPKHPIVDQINNSQLDLFLNSKTTPRKNNNENDNNSNNNIDNVFHILNNDSYHVTRLFYYISESKFQFNYLFFVQTLDDYKNILIKLDKNFSHSAIVGDRQLIDLFNSHVNNLTSVLIIYHRDFYDKYKYEIQYKNSNIKTIIITNDSDFKSNHPNVKIFGQHVLEPLSNEGLELLFNNLTDSIVSKDDIFYKPLMFIITQVDLEVMIEHCKDIRNKTRTPNDIIYKELSNGSVTIAPNEIKPEKIIFQFSRSYATFTIAPSEIKPEKIIFKFPVCEMLCSFEVNYLKSQTKQNNESVPITIMQEPKQCTNPYQLIFTSEYAQIIVNNKTNGDVYNIKFDLLFIKACYKLHDREMCINLIPLSGTLESRSQGIQSLSLSNCFGTILYRQSTKYYHKISEKKIEIDAIGYSFSTNNNNNNNQNSEKTIAIKLESGKKRINFINVEGNIHFIGGVKFIKDTDHTFIITSKYSRFANNNQLIFKNKDNQPIPDRGSLNISGNLCGDIILKDGTIISFDIPAYINRKIRNKLNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRX0</accession>
    <name>A0A8J4PRX0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_006416</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000295">
        <property type="protein sequence ID" value="KAF2072262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRX0"/>
    <dbReference type="OrthoDB" id="275301at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05122">
        <property type="entry name" value="PKc_STE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001158">
        <property type="entry name" value="Probable serine/threonine-protein kinase mkcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF22">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SAMKA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="362"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="230"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="72"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="114"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="140"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="201"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="230"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="246"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="392"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2072262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="638" mass="71474" checksum="48E715B7034B7246" modified="2022-05-25" version="1">MKLFKKAKQHFFQTQSGAESPQDPEQVEKVLKDINPNNSTGPQGYIVYCETPDGGAPKESSETTPSTTPSNDGEKKSSSADKPYVDEAHHIEEKRAHKDQSSEATHNTTDEQPQPQPPTPQQQPPAVVTTKERSKSISAPSNTSNLNNNDISISTTTPSPPTNSSDHPSSTTTTNSESQQSPQQQPEKNGATPTTPPPPKQSTAIQQSSSSSTTSLLASTLSKFKIKAFKSKDKDKEHASDKKIVNKNASVSVNNNNPPQKTTPDQSKAEEKQPEKEIILIKSLEDFPDDCQKLIRISQIPEEKLRNNLAILAHVLHFRTGKQFKLEHDLPKEPRKKFHSEKFNDGETLLEHYEPAQLKKLYKDCDQVGKGGFGTVYFAKSTKEKRMVAIKKMPHITKRQIQQNYREASILSKCDHPNIVKLYTCHIDKDSNLWIVMEFMEGGTFEEAAKAWKFNENNLAYVARELLKGLQYLHENGMVHRDLKSANIMMSVEGKVKLIDFGLCEDISTSTPCHMVGSPFWMPPEMIQQKPHSTPVDIWSFAISLLEMANQRPPMMESAVKAMFTVATEGATSFDEPELWSDCFKEFLSLCLKMDPAERATAADLLKHPFIKKADSRDNMENILKKIFLTNSLMNSGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS25</accession>
    <name>A0A8J4PS25_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006716</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000322">
        <property type="protein sequence ID" value="KAF2071960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS25"/>
    <dbReference type="OrthoDB" id="21152at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF5">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="262"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="2"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="305" mass="33809" checksum="233A169D6C80D49E" modified="2022-05-25" version="1" fragment="single">TCDCQQGWESSDCSIPNKGGVIPDPNINPNDTSSTIITPSGAIFDIGIVLINELDNSNNVILSYNINSISWNNITKQDSVYSYTTNLPNTKSTLNVQLTVNPLNERVYYNFAGDVIPILPKSIKYQVELQNYTFSSSLNTMEFVFKSGIIDKGDECVYDESTKTQTTTDDSIRSIQMTLNGETLVGTFSDRIVLDDRPSYNKVNQLTDDQIIKYQLNQESLYVSIATTYFKSSVVVDPNFGVLVSSNPDTESCKNKFASWKIAVIVVCSVGGVALVITTILLIKKKRVQSQFEAKLKKLNGNINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSV5</accession>
    <name>A0A8J4PSV5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005824</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000243">
        <property type="protein sequence ID" value="KAF2072847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSV5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="51" mass="5218" checksum="98A567F3ECDC3B3A" modified="2022-05-25" version="1" fragment="single">VTSAPTDTPSATPTATPTATPTEKPHGNSAGVLTPQIFLLFVATALFSLMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSZ3</accession>
    <name>A0A8J4PSZ3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_006162</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000272">
        <property type="protein sequence ID" value="KAF2072519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSZ3"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000080">
        <property type="entry name" value="Ras-like GTPase Ras1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2072519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="199" mass="22606" checksum="39663F583B7DFF83" modified="2022-05-25" version="1">MSVSNEYKLVVMGGGGVGKSALTIQFIQNHFIEEYDPTIEDSYRRQCQVDEDTCLLDILDTAGQDDYSAMRDQYMRTGQGFLCVYDVTSRTSFEEINLVRDQIIRVKDNEKVPIVLVGNKCDLENLREVTHGEGEELAKSFICPFLETSAKKRLNVDECFFEVVREIKKSLKEPGRSKKDKKGVQGYLKKIKGGDCIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT53</accession>
    <name>A0A8J4PT53_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_006124</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000269">
        <property type="protein sequence ID" value="KAF2072549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT53"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12122">
        <property type="entry name" value="AMPKA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14079">
        <property type="entry name" value="STKc_AMPK_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000544">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000236">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032270">
        <property type="entry name" value="AMPK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028375">
        <property type="entry name" value="KA1/Ssp2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001772">
        <property type="entry name" value="KA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF110">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16579">
        <property type="entry name" value="AdenylateSensor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103243">
        <property type="entry name" value="KA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50032">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="31"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="KA1" evidence="13">
        <location>
            <begin position="544"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="355"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="357"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2072549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="592" mass="67297" checksum="CD303B26D33DC78E" modified="2022-05-25" version="1">MSNMYPNNPYGGNSLWNSGGIEKTSLLIGNYRLDKTLGIGSFGKVKLAEHVKTGTKVAIKILNRTKIKNLKMDEKIRREIQNMKLFRHPHIIKLYEVIETPTDIFMVMEYVTGGELFDYIVKNGKLSEDESRRLFQQMISGVDYCHHHMVVHRDLKPENLLLDPLHKSIKIADFGLSNMMQDGDFLKTSCGSPNYAAPEVISGKLYAGPEVDVWSCGVILYAFLCAKLPFDDENIPTLFKKIREGVFTIPDFVSSQCTDLIKKMLVVDPVKRITINEIRNHPWFQVKLPKYLSSPNTFLSKSIQKIDHDVLNELVKIYSPLNSQQLIEELQKTEVNDYIVSYHLLVDSKRVSYEVESPSNNHTPNHSCNNSTTTTPLLSSNKVVNNNNNNNNNINNNSNINNNNYNQEQFELEPDIGTTTATTSIVVDNENDLSNINNNDNNVNNMDGPTNEGGAESLNIEESNNDIIINNNLSNPINLNNNPNFQISSSIDFNADSANQPHRKWYLGAISQLPPHEIMGEIYRALKKVGFEWKLTGPFQLRCRIANNKPVKLVLQLFRVAENRYLLDIKKIEGEIFIFFDICSLLLEELNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT88</accession>
    <name>A0A8J4PT88_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SCP2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006583</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000310">
        <property type="protein sequence ID" value="KAF2072109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT88"/>
    <dbReference type="OrthoDB" id="5327538at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="entry name" value="SCP2 sterol-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10094:SF25">
        <property type="entry name" value="SCP2 STEROL-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10094">
        <property type="entry name" value="STEROL CARRIER PROTEIN 2 SCP-2 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SCP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP2" evidence="1">
        <location>
            <begin position="6"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="SCP2" evidence="1">
        <location>
            <begin position="149"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="247" mass="25978" checksum="37DEF1DEC096A409" modified="2022-05-25" version="1">MSVKFFEDLKATIAKDSSLLKKINGIYQFNIKNGDATKEYIVDLKNAPGSVKEGKHEKPDCTIGVGEADFVEILAGKLTEQTAFMKKKLTLKGNMALGMKFRNLVNAVKANSSAAPAAPAAAAPAASGSLDDGTKIGKVFDTLATVIKGKPELVKSIKGIYLFDIETASGKKQYTADLKNGAGSVSASAVGKPDCTITMKEADFLDLMSGKLNGQVAFGQGKLKIKGNMGLAMKLGQITKATPKAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTZ9</accession>
    <name>A0A8J4PTZ9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005594</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000227">
        <property type="protein sequence ID" value="KAF2073100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTZ9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="87" mass="10295" checksum="C9A6AC0AA0244B8B" modified="2022-05-25" version="1">MDKEDQDQVLKQRDLDLNLQKEALTFRINNELKEINKNMDILINVNLKELKERDNFIKRFIETLDLQPIDDRLTTTTTTTTTSSLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU65</accession>
    <name>A0A8J4PU65_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Lipocalin/cytosolic fatty-acid binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006024</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000259">
        <property type="protein sequence ID" value="KAF2072664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU65"/>
    <dbReference type="OrthoDB" id="565904at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19438">
        <property type="entry name" value="lipocalin_Blc-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002446">
        <property type="entry name" value="Lipocalin_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047202">
        <property type="entry name" value="Lipocalin_Blc-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022272">
        <property type="entry name" value="Lipocalin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10612">
        <property type="entry name" value="APOLIPOPROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10612:SF34">
        <property type="entry name" value="APOLIPOPROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08212">
        <property type="entry name" value="Lipocalin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01171">
        <property type="entry name" value="BCTLIPOCALIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00213">
        <property type="entry name" value="LIPOCALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035285887" description="Lipocalin/cytosolic fatty-acid binding domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="Lipocalin/cytosolic fatty-acid binding" evidence="2">
        <location>
            <begin position="40"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="581" mass="67282" checksum="10CA53A2E3BD1511" modified="2022-05-25" version="1">MSVSPSFKLSALVMFGLILSVVKSSYTPSGVTVVQDFQGERYLGQWYEIARFNHIFERGMDHVTATYSTRPDGGLDVLNRGFKVEKNQYEQKQGVAYYVGSNSTATLKVAFFASIYSGYNVIDTDYETYSLVCGPTRDYLWILARTPSLDKAIVEKLEALAQSFKFNTNNLIFVNQEPQSEATQEIGCDEIQTLVNTLYDSVNQSSRWDKESCQRAFMWIQHFEEYVHYLLARIDIDQQYQIELNKFKENIDTIIKNTSVTLNRVPLSLINSDAVIQCSLSNHLLSQWLNNIFLTEDQLDLFLSSYLEYSNEKRDEDDGDYIDYHKKRKERLDNALNIFIKISNSNNYIKVLKNLSAYCLKSTTATTNINSNIDQPNLHINNNLELRATAQLVLKYIDVQTTNNQQFDKSVLYLSIIKTIQHHNLLIWVIWLLISRPKEILHLSFIKSTNTQDSIQEIINTFNSIKGNISKLKFFLDSSNQELLSLACRMNAQIYQLQLSTIFLLIDYYIFDEKASNSWLSVDYQSKIKQSFTYILQHQPNYHKSTLLHEINQRILSTKLKPFNTKNNVLPILIEIKKKCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUC1</accession>
    <name>A0A8J4PUC1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_004632</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000167">
        <property type="protein sequence ID" value="KAF2074063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUC1"/>
    <dbReference type="OrthoDB" id="20786at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000499">
        <property type="entry name" value="Serine/threonine-protein kinase KIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF28">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PAKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRIB" evidence="16">
        <location>
            <begin position="118"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="149"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="426"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="535"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="645"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="14"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="441"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="463"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="474"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="559"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="645"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="677"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="688"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="718"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="762"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="778"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="788"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="844"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="874"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="912"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="929"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="944"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="963"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1007"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1034"/>
            <end position="1052"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2074063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1093" mass="117993" checksum="DC39EE2F3845B596" modified="2022-05-25" version="1">MIKNVVSPMMMTPKKSMDEQLKEIEVLKQQLREEREQLERDKGQFDEDRKIIFDSLSKCIGSGSANIGKNISKALKKAEKVSKKANGAGNGGHHSSMPLASVEISNLTHSSGQIGQSIGTPFNVQHKVHVDFDYKWTTAIEGQDFSQVFEFIETLGTGSYGTVHKAIHKDTHFTLATKVIPLQDSEEIEKEISILKKCKSPNIVSYFGSCHQGENLWILMDYCSLGSIRDMLDLSEKTLSEKQVAVVVQQALKGLHYLHSNQIIHRDIKAANILLNDDATVKLADFGVSAQLDDAMSKSTDFIGTPLWMPPEIIQKKPYNNKCDIWSLGISIIEMIEGYPPFWKMPPTRAMLMIPNKQAPTLTKPAQYSKELNDFLSLCCQKDPEKRPSALDLLSHAWMAANSVASNIEVLRPIIDECLKKASARKKKTAELQKKSGGVGIKEKQNSKDSKGSAGSNPDEADSFNTFILKTTVNNSNSNNNNNNNNNNNNDSPCSSEDELDCGTMITKDDDDNDTNASATTIPAFIAALRKTLTKENQVSRSPPSNPLPPIPNGVNKFSNSNNSNNSNSSNSSNNGNTSTTNTNLQELLDKNQDTLKEYIQQQLDKLKKDILLDVSNLLNEKLSSVNSSPSKPTTGGFIQLSHASSSNNVSNNSNSNSSSVSNIFKKFPTPPPAPLVSSQKNNSTQISDNVNSSNNNPTPSKPPIVHNNRRSREMDSVINNFNNLNTRPPSPKPNSFNRPNSPFRKSIGANNNRPLSPPPNNNNNNNRPLSPQPNNNGNNRPLSPQPTTTTTTTTTTTTTDNETIVNGEPISPANRAKPQPPPPRQPVGKPSPKRAPSPSVRRSTSLSPQPVVSSPSKERLPPPLPPPRVGGASVSSPSTPLNSSPSASKISAPFNVSKSPGSPYVPPRPRTPSSSSPSQSGAPLVNKRLSQSMTSSQLKSQLHGSSSNGSNGSHGSAGPSIGVSTGNTKRLSSTMSPILLSSPGGLGAPKQPSSLRTSGGSGFSIEKPHTPSAEKRAIPVPSPAPSTKSFNYTPSTPSTTTTTTTTTTTPPLQSVNSKDKDNLSGSFRKKSVIKPLTSVKSIFSPLKKVEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUN0</accession>
    <name>A0A8J4PUN0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004885</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000182">
        <property type="protein sequence ID" value="KAF2073791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUN0"/>
    <dbReference type="OrthoDB" id="2157498at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029164">
        <property type="entry name" value="PIG-Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36485">
        <property type="entry name" value="OS01G0939000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36485:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15159">
        <property type="entry name" value="PIG-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="77"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="129"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="249" mass="29025" checksum="6A38AE4627C5692D" modified="2022-05-25" version="1">MNDMMSTSPTLMGRMRMNNITPLSPTQQQHQQHQQQQQLQVSMQQHLHYRHFYNPPHHATTHGMTSFSSPLSPTSAQQQQHHQQLQQQLQSLQNSQLNHHSSNTGGSHNHSHHHHHHHHHHSSNHIHTSHSNTPNNSVSPTTPSSTPAPPTINAAAPVRKKYKPMQRYNTVNHDRTAFYGWIILFCTFIFFISSTYCLFFSKLLPDTGNKVLDFIKYDWYYCFLVPVLIPVTIITVYFNWLSLKFFRHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PV15</accession>
    <name>A0A8J4PV15_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_008536</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000534">
        <property type="protein sequence ID" value="KAF2070151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PV15"/>
    <dbReference type="OrthoDB" id="6375174at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14501">
        <property type="entry name" value="PFA-DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000035">
        <property type="entry name" value="Tyrosine phosphatase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004861">
        <property type="entry name" value="Siw14-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF18">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03162">
        <property type="entry name" value="Y_phosphatase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="304"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="20"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2070151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="332" mass="36795" checksum="BB4AAD2161647519" modified="2022-05-25" version="1">MSSTNNDNELKSSSNIKYKTTPSNSLPSVLPTTTPSTPSKKQTIQQQQTNVNNPSSTTTTTATNTSTSSSSTTNTSTKNTNSSSNANNHINTLLNSGPPQLSPPALFGIIEPQLYRTNSLYPVNFPFIKMLGLKTVVQLSPEVPIKAVSNFFQENNINLIHLGLKSWNVDSSWKPLTDELVKECLEIVLNYDYYPLMITCTSGVHQSGVLVGCLRRIQNWNLTSILVEYKAFSGQSNSRYVNEQFIELYDVDLVTLPNKLPTWFVEQQKMLEEELENDNPDEFLYSTSNSAKLSKSYYYDMISTSPTPESSSSNTPTTANTTTTATTTNTNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVT0</accession>
    <name>A0A8J4PVT0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cathepsin propeptide inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_004087</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000138">
        <property type="protein sequence ID" value="KAF2074617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVT0"/>
    <dbReference type="OrthoDB" id="20728at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035211964" description="Cathepsin propeptide inhibitor domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="3">
        <location>
            <begin position="33"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2074617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="223" mass="23936" checksum="74A874BCE80E0257" modified="2022-05-25" version="1">MKIQVVLFLILSIVALISSQDYTPTEQELQDTFLSWMQAYEISYTSEEFQAKYNQWKLNLAAIAKNNADITQPDGVFNQPKASDALLANADFAFEAEPVVTYPEASQSQLSLNRFSDLSYTEFVSTYAGADATLAATAATAAVLSTGAIIGIAVGGAAAVGGVAGATVYVVKKKRSNKTKTVAAETELEQPSSSNDNFKRQGATNIFNMDNPHHSITARAAYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW98</accession>
    <name>A0A8J4PW98_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_003493</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000110">
        <property type="protein sequence ID" value="KAF2075188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW98"/>
    <dbReference type="OrthoDB" id="10260946at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034709">
        <property type="term" value="C:methylosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052139">
        <property type="entry name" value="Methylosome_Comp_WDR77"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46853">
        <property type="entry name" value="METHYLOSOME PROTEIN 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46853:SF1">
        <property type="entry name" value="METHYLOSOME PROTEIN 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="118"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2075188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="322" mass="36773" checksum="0205E19495A6A728" modified="2022-05-25" version="1">MTNKNLQTTEYSICSVDYSLDNQWLALGTSNLTNSIWDGKIILFNDNADVKEKQYDVDSGITGVQWLNNDILVFSTDDSRISFININHSNQTTTPITITKTQDYIEDDFKNRGISQHLCCHSHIISSIDIFENKLLSSSWDKSIKLWDINSSDETSLQLSNQFIHHYKKVNIVKWNRINRDLFASGSDNGVVVGDCRDKHSSVFNKETQIPVYSLAWATNNDYTLYTGHSNGQLIQYDIRNTTEIIKKPHSLAINQIHVNSKYISTVSDDRSSKVFDINNTDTLVYQYESNDFIKCITSKSDSTNQFITGSIDKKLVLHQIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWD4</accession>
    <name>A0A8J4PWD4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003844</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000126">
        <property type="protein sequence ID" value="KAF2074856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWD4"/>
    <dbReference type="OrthoDB" id="76773at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052050">
        <property type="entry name" value="SecEffector_AnkRepeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586:SF3">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="557" mass="64481" checksum="B65EED4EAE946B0B" modified="2022-05-25" version="1">MDRSQHHQHHHQHHQQHHHHHHHQQQQRHQHQSIPLYELLFKKIFKNSVLFNHIFSFIRGTGTPYYKIFEVGWMLKCGHLGLLVDKIHLRELNGSIHNNNNNSKSTGNNGPYRLRTVKSTGDEHILELFFKRVKSFKEFLYIYTNTLTNCSSMVILSCHQPNIEIVDYLYREKEAKAEAIFHSKGIYNAAKHGYLDVIKYMTEKEKLFPSYDFSFIDDKLFAIDIAAEHRHFEVVKWLHYNRLHIKNCSSYAMECAARKGYLEIVQFLHEHRKEGPSKNTMVSAASQGHLEIVQYLHENIPTIHCTQDAVNYAAENGHTQVVHYLLTHRKEGCTSYAIEFASLNGHFDIVKLFSPINSAQATVNACINNHYDIAEFLIKTHRLLPTPHGFDYTCFKGNYAIVQLLHESGATCTKNAMDLAAQMGHLEIVEFLHLNRKEGCTTNAMDLAALGGYSQVVHFLHGNRLEGCSTNAIDNAALRGHLDIVQFLVANNYPYKNALLNAMEYSKKEVFNFLYQRLDLKSSSQDKKMHQDLVKRSQLLPSTHDYRNFFRQNDNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWV9</accession>
    <name>A0A8J4PWV9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_005283</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000206">
        <property type="protein sequence ID" value="KAF2073404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWV9"/>
    <dbReference type="OrthoDB" id="346907at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007254">
        <property type="term" value="P:JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009967">
        <property type="term" value="P:positive regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46716">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46716:SF1">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="636"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="200"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="306"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="360"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="566"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="912"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1056"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="200"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="330"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="371"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="928"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="954"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1059"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1074"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="663"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2073404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1228" mass="137112" checksum="4C2995BE91D0BB6D" modified="2022-05-25" version="1">MSTDTNNNKSLVRTRSISDGAPKEPPTKRAFRFYWANDSHMYWTLLLDYSNTVSDVNEGLIGKIPTRGAQCALYKRTKGNFGRVKDRRLSNEDVIWDIVDKRRKNRKSDPLFVLKAKKPSSLSPPSSSPTLTFTSISKKSIELEITSPLSNSGEILNNNINNINNNNNNNNSNSNNNSGVNYNPYITNASISNATVVVSNTKSNNNNSNIVNNNNNNNNINTQISNNNSANSNSNTNTSNSNSSSPIMGVSPPQTNSLKSTMEMDPPPIPTISGTTNNNNNHYHHITNEKPPLSAAAILNSNSYSSNSLKSSATEMLPPPPSLTNSIIVTPPTTTTTTTTSTGRPLSSSSLANIIEQQQIGPTTTPPTPPSNSNSNNNSSSRSPLSGSNGISRSPSPFAKKVGGSILDHRKSSRLSGQLNPQDIRRLVKFYFGDSENTFLTIAVRNDMQSKEVCGSVEQKLLLQKNSTYIALVMPGNQNTPKIEKILEDDDVVIAIKDSWQDPFLTYFHVHQKPQNSSGKRLSRQIHNNEPHVIPSEWRRSTDAVTLKTSGEDMWNGKRKSIPIFLQVAGPPNSPTSSAVPGSPQRHHHHPTSPLSGANPENELQSGGDGANDDEEEEFDLLQLKGWIHWIPSADIEYIKRIGSGTYSKVYKGKYQNKFVAIKTMRGSNMTTEQIEGFKKECDILSTIQSPHLISFYGSCIEESQLSMVVEYCSKGTLYKVLNNPLLDFDWDKFFKWTIQIVEGVKYLHNMNPPVVHRDLKTLNILISSEWNAKICDFGLTRTMTMTNVSTLGTLRGTMAYTAPEIYEGLLFNTRSDVYSLGIIFWETVNRCFSGSYLKPFHDHPISLDIQIIILTSRNKIRPILNQHWPEELRHLIAWCWDEVPDKRPNCNEVYDYLIKLKQTFEDNRDTWEKIRTKSPKQVPIGGRSSSSTASNANGVSTKSNIDGGDNDDVTEKPADDASKQQMEKEHLEDHHQIGQQQDHLHLQFNDHHLLLDMEQQQQLEQEVLIQHEQQIRHEKQPVTVTRERKNTIIESSNATLIEVHSALVTEVFDYEDPQSPSLDSNSTNASPENNGNCINSSNSSSGSIPDILIPLNLNDNEDLSPSNNNNNDNNNNNNNNNRHSPLSSTSSLSPLSSFSPELNPLESNQIFEDKTCINNQQQQSPLPSQSQIVCDHVNIQTHHHQEIENNNNNTNNNLSNDENNDSQTLEEITTNNLFIDDNVNVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX51</accession>
    <name>A0A8J4PX51_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003825</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000125">
        <property type="protein sequence ID" value="KAF2074870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX51"/>
    <dbReference type="OrthoDB" id="23602at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050872">
        <property type="entry name" value="PPR_P_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128">
        <property type="entry name" value="MITOCHONDRIAL GROUP I INTRON SPLICING FACTOR CCM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128:SF211">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="876" mass="102338" checksum="B6AA2755AA9AF0B3" modified="2022-05-25" version="1">MNQLFQSKVYKTTTSLFRVAFTSGSSSGMGSSRLYCNTSLPHSTSEKEDSNTTTEINVSTTKQGSVNENAIYLYPSQLWIKDKVLSSNSNNNDNNSKNSNSYNKEFFVSRNSISFLPEILFPLEPTPPNFHRSNPISEENISVKTEFEHQEYNDILDIITNGNQVQRPLLVNDLEKAYNIDRNKTHFIFETDLVAREYTGKQVNDIYQWIKNTQHFNQPSVLNTFLQYYAGNTLDNVEFESLLSRHKAKEIQFDCVSFSIILNHFCEKQDLESLNYWTKVAMETCKKEMIENRLLGIALVRAYLTLSDIDSAVNILKLFKDSKSTILNHICYRSLHLFVHHLTPRKMYDKLLTIIKTHVAHSPSFLFDGIFYKLLIELWKNEYSVDDLFKYLEKTALFKAEVFYSEFIYYLLGKDQIDIAQSVHSMYLETHKPSFRIYNVLMNHLIKQDDYRSCLNLVKQMESHKLQLEPKTIVAILEMLERNNKTDLMNGFIKYLYSCPSFWIKMSLVIIHSEIYSNDLVFNKLVDRIHKLQMKEQSILINNIIRDCLKLGHYHSALRWYGVRLNEFRLKPNKYTLDFFRFYHEAAIKKLENTKNSAELIEKEKALINFWNSRYQHFDFVRAADETKAQEEYNNLRVLEMIKGIDQLTPMGLLKSQAMKYTPNFLALKQPTQEMESIRKDFIKQNTLLNGIKDTNLETSLSVFSQNPDNQANLNDLCRQLLEYIKKDTLPFGTVFLQAMVEILSKAPDRYISLLKKCSPNIRSALFDIEFYCDLIKKDVSTATSLLFQENPLFWRESDPIWNCLIISLYAAGRSDRAAKMIDTAKETNRVLDEVKIQECKDNPVWAPMSSEALDFITYNFIKLPLKKKLQNKKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXA0</accession>
    <name>A0A8J4PXA0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CNH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003745</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000121">
        <property type="protein sequence ID" value="KAF2074960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXA0"/>
    <dbReference type="OrthoDB" id="5325112at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032914">
        <property type="entry name" value="Vam6/VPS39/TRAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019452">
        <property type="entry name" value="VPS39/TGF_beta_rcpt-assoc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019453">
        <property type="entry name" value="VPS39/TGFA1_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12894">
        <property type="entry name" value="CNH DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12894:SF33">
        <property type="entry name" value="TETRATRICOPEPTIDE-LIKE HELICAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10366">
        <property type="entry name" value="Vps39_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10367">
        <property type="entry name" value="zf-Vps39_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CNH" evidence="2">
        <location>
            <begin position="19"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="1">
        <location>
            <begin position="540"/>
            <end position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="862" mass="99285" checksum="144804233B7CD23A" modified="2022-05-25" version="1">MSRINMNSFDPISVIDKITQRIECIDTWEYRGSKYLFLGTSEGHLLIYDVVEKNVQGKITSSVMLKDTKPISKKTPITQMNVFDDFNILVILTDSEIRVFNLINFSVIAILQKTKGCNTYAYSYKAGEYLHLVAAVKKKLVLYSWDGSDFLEIKEYNIPDIAKNIDFRDNAITVCFKKAYNIINIQDGSAINVEADKLSFTIFVQEREFLIVKQNMAFFINTEGSPNRRHSMTWLETPSAMAIHYPFAISIEPKQVEIQIIPDPKDPKTISQSLFLLGCKSITVKKDIYVSSPTSVWRLAPMPLMEVVDEMVSNLEYETAINLLSNTQENVPGIKEKLIKIKTKAAYHLFSKEQYESAMNYFESAQVDPLKIISLYPGLLPPNLQQKLSVPFQVKDTDKNSQALKELEKYLVGCRKNKMTYTPPELSNSDYDLPTLVDTTLLKVYIKGNPQLIPHLFKVKNYCHIEESQRVLLEEKKLNELVLLYKSKELHREALTLLAKTKTPKDTISYLCQLGKQNMAIILEHSKWVLQKCPEDALTIFTTERKEDALPPAQIIPHLKHFAQPLLRQYLEYIINDPLNPDKTPEFHNDLVFEYLNAILELIKPDKCVVKRVPGETIAGTEPGLLGVLRKKLINFLETSKYYLPEKMLSRFPFDDLYEERAILLSRIGRHESALAIYAHKLKNFQMAEEYCDRNYNKDSEDAKDVYLSLMNVYLKTDGVEPLIDPALRLLNKHYRSINTPKALNLLPTNIPIDELYPFLEAVIRDNTKTKRDNQIIKNLFKSEHIKIKEELIHLRSGVIKITDDLNCAVCGKKFLGTQAFAAQPNGTAVHYVCFKQDKYQQDRFQSQQQQQQQQQQNGYYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXH0</accession>
    <name>A0A8J4PXH0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Lysosomal dipeptide transporter MFSD1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Major facilitator superfamily domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">CYY_004115</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Lysosomal dipeptide uniporter that selectively exports lysine, arginine or histidine-containing dipeptides with a net positive charge from the lysosome lumen into the cytosol. Could play a role in a specific type of protein O-glycosylation indirectly regulating macrophages migration and tissue invasion. Also essential for liver homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-alanyl-L-lysine(out) = L-alanyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79415"/>
            <dbReference type="ChEBI" id="CHEBI:192470"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-alpha-aminoacyl-L-arginine(out) = L-alpha-aminoacyl-L-arginine(in)</text>
            <dbReference type="Rhea" id="RHEA:79367"/>
            <dbReference type="ChEBI" id="CHEBI:229968"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-alpha-aminoacyl-L-histidine(out) = L-alpha-aminoacyl-L-histidine(in)</text>
            <dbReference type="Rhea" id="RHEA:79375"/>
            <dbReference type="ChEBI" id="CHEBI:229967"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-alpha-aminoacyl-L-lysine(out) = L-alpha-aminoacyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79383"/>
            <dbReference type="ChEBI" id="CHEBI:229966"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-arginyl-L-alpha-amino acid(out) = L-arginyl-L-alpha-amino acid(in)</text>
            <dbReference type="Rhea" id="RHEA:79371"/>
            <dbReference type="ChEBI" id="CHEBI:84315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-arginyl-glycine(out) = L-arginyl-glycine(in)</text>
            <dbReference type="Rhea" id="RHEA:79391"/>
            <dbReference type="ChEBI" id="CHEBI:229955"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-aspartyl-L-lysine(out) = L-aspartyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79411"/>
            <dbReference type="ChEBI" id="CHEBI:229953"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-histidyl-L-alpha-amino acid(out) = L-histidyl-L-alpha-amino acid(in)</text>
            <dbReference type="Rhea" id="RHEA:79379"/>
            <dbReference type="ChEBI" id="CHEBI:229964"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>L-histidyl-glycine(out) = L-histidyl-glycine(in)</text>
            <dbReference type="Rhea" id="RHEA:79395"/>
            <dbReference type="ChEBI" id="CHEBI:229957"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-lysyl-L-alanine(out) = L-lysyl-L-alanine(in)</text>
            <dbReference type="Rhea" id="RHEA:79399"/>
            <dbReference type="ChEBI" id="CHEBI:229954"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-lysyl-L-alpha-amino acid(out) = L-lysyl-L-alpha-amino acid(in)</text>
            <dbReference type="Rhea" id="RHEA:79387"/>
            <dbReference type="ChEBI" id="CHEBI:229965"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-lysyl-L-lysine(out) = L-lysyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79403"/>
            <dbReference type="ChEBI" id="CHEBI:229956"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-lysyl-glycine(out) = L-lysyl-glycine(in)</text>
            <dbReference type="Rhea" id="RHEA:79407"/>
            <dbReference type="ChEBI" id="CHEBI:191202"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="18">Homodimer. Interacts with lysosomal protein GLMP (via lumenal domain); the interaction starts while both proteins are still in the endoplasmic reticulum and is required for stabilization of MFSD1 in lysosomes but has no direct effect on its targeting to lysosomes or transporter activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000140">
        <property type="protein sequence ID" value="KAF2074569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXH0"/>
    <dbReference type="OrthoDB" id="424834at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052187">
        <property type="entry name" value="MFSD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512:SF4">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="20" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="198"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="472"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="507"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="537"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="562"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="594"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="626"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="21">
        <location>
            <begin position="156"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00044876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00044878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00045018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00045709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00046376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAF2074569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="660" mass="72946" checksum="598AD7FAC8C5B07F" modified="2022-05-25" version="1">MFDYVTNLNDSNNSNQSSKNGIMTRGNPLQEDLNNDDDVERLIVIKSKHNTIPLSLSTPPFNNYNSSRNILQISSEIHPSTLGEGLNKPVLKSILKSNNNSNNSSLNNSAAIIFNSQTLGEENSGTQQDDMGILNNSISSNRNIFRSIKNIFKLHNLKTFLLVFLISNLGYSTFFSYTSAEALSYTFYQTFNISSSQFGWIFTAYALPNLVMVFASGVLVDKIGPDKVSIMLGTCVTLSTLIGALSPPNFTLMLVSRFLLGFAGESLVACSNTLMSKWFSSSSLPIYMGILVGWIYSANLTSLTLLPLLNKRFGFQTSLWIISFVAIFGYCLNIIYLLTRSFFKDVKEHSSNSNNSFKELKEDEIEVDIIKDDQETLESYSPNGADLMPNSSFFGLDNNDGENFKDGLIGSSVELEEIDIKQTPSLAQSRVIEMSNSHHGRTINELHEIIRNRVGNLVIGITSVFKDIPQRMWIVVLIVLFGYSTLFGLAIIGPDFLGTKYGYDEQMAALILSSETISSALFSPLCGFGLKYISKRIIVLWTSLALLGVGVLLLVITNIFPLPWILVSGIGYSILNTTIISSIPTLVPEKSIGASYGLVGTAYNTGLVLFPYFLGLLKEKSGDYTLSLLLLVLFSIIASLLVAYLKYLDTKEPIDKRLDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYY5</accession>
    <name>A0A8J4PYY5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_002685</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000076">
        <property type="protein sequence ID" value="KAF2076025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYY5"/>
    <dbReference type="OrthoDB" id="1903104at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15545">
        <property type="entry name" value="PHD_BAZ2A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45915:SF2">
        <property type="entry name" value="TOUTATIS, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45915">
        <property type="entry name" value="TRANSCRIPTION INTERMEDIARY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="7">
        <location>
            <begin position="333"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="41"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="421"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="497"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="556"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="643"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="124"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="145"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="163"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="201"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="271"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="298"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="427"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="459"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="522"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="556"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="572"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="582"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="592"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="606"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2076025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1140" mass="130756" checksum="4A60EAB2C8C6504A" modified="2022-05-25" version="1">MVRCTICNHCYHLSCLTIPLTKLPRMDWFCERCTNNSLNRNLKRGGRGSTKDSSNNESSSSSSSDDSDDSDDSDDSSADNSVDGSNNDEKLSETSDDDEIVNNHRKHKRNRRNIINDDEDDDSNGDKDNVNTNGEKIENNESFKSNRRHRNRRKNKVKHNSLDDGDDDDDDDDDDDDDDNDDNNERQDQDEEEDQDEKDTKQKKGNRRRRNRQSIKKRENNSESDSENGVEDNQDNSSDDDDDQLNSRRKRSRSSSTTTKKNLRRDIVNSRARKRRKRKIKSIVTNSNENESSDDSNNKNNSNHNNNSDSDSNSNDSDYSYNSSDEEEEYLDDLVCSKCNTGKDEDKILLCDKEGCSRGFHMYCLKVPITSVPKGDWICDHCKYESDDIDEDGQIIQTNNNTSSSSISILDKKEIQPMVLKKSPIQSSLKSSSNSLNKSNNSIMNDNNSNSINRDKENSLNNSNISNNSSNYNKTDLTKTKTGLLVPNVIPVNRSSKDIKKSSAATQFKHRKEEESILDMIPSTSKSIPSNPISWDFSSSISTNSTMKKDNNSFLNYKSNNYNNSSNNNKSTLPMKSSVKSNNNINSNHNHNNNHNHHNHNHNNNHHQNNNHNHNHTHNQQQQTQPQQNLSISEQFTVHSPPLALSPPISLSSSSSSSMPISQQQQKPIWTGKIYFQDHFISLSNIFKLNQQQQNSNNSLFKLTSIHFNEINFKELPIKVQKEIQKPLQILSFSLNQTLFKHFDQQVLLISPEFENHSSFYNLILKLKSKQLVGSFSFDDSIIYIFPYQTTILNLDLIAFIFKKDQVGNNNTNQQELQQQQQQEFDQQQNNSFLDKNICIIGYDNNLNIIKVKKALQIIGSNIIDFEIIQNTSTETTSMDLDLSDNNKNNNNNNSNNSSTGDLNIDMFIVDPNFISKLYNIKNLQKFKNNQNTIFIQDNDLVYIQNIITTTKQYPKSFNQIITTTATTIPISPKILFDNNQAVFLLDNNTIIESEYSLFIKMINLLLKQRSFSNGNVYNNNNNGKEWIIMLSQNFIEDLNNNNINNINNNNDKNIDMKRKLATIQKHFNQIIFPQPFNPPPLESSEFLLIALRISKVLIQNYRHVILVSSNNIIQRESLQYPSIACLSMIDSINFINKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ65</accession>
    <name>A0A8J4PZ65_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RNA polymerase II subunit 5-mediating protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_001785</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNA polymerase II subunit 5-mediating protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000045">
        <property type="protein sequence ID" value="KAF2076883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ65"/>
    <dbReference type="OrthoDB" id="21413at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019212">
        <property type="term" value="F:phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23159">
        <property type="entry name" value="Prefoldin_URI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052255">
        <property type="entry name" value="RNA_pol_II_subunit5-mediator"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00293">
        <property type="entry name" value="prefoldin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15111">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT 5-MEDIATING PROTEIN NNX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15111:SF0">
        <property type="entry name" value="UNCONVENTIONAL PREFOLDIN RPB5 INTERACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="173"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="402"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="510"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="124"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="173"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="211"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="273"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="407"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="430"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="526"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2076883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="536" mass="62616" checksum="A026F091EB6435A6" modified="2022-05-25" version="1">MNNQHNHSGHHHHHHHDGHSHDHHHHHHHHQQQQPKLTPRQEIFQKYNQKIVEYQEHIEHLNGTKKEYSELISTLEDLPDTLKPKIMVPLGKLAFFEGNIKNSNQFMVLLGDNYFTKRSSKQAIEIFQRRDRDIDENIKDLNEQIDFIKQKFALTTDLSKALGEKNDENIIEIKEDYDSDQERDNNNQREIKKKEKSAEKQQKKQMQQKEPPTKEAIQKKKDRDDEFDEMMRKLEILEEKERKSMEKGELDDDEDTSAAATLMKNFAKKLNMTDDDEEDEEEEEEEEEEYQEKEYNDVDETVFADKEGDYGYGHNDDDDDDYNDVIEYYDEFGNLIDINDPNVEYIQDDGNDDEDEEEIEINEYQDGTDELYLNKNGHDLNEQEVKELESIWQEKRKTLGKDILSATNTPTTTTTTTTTTTNTSKNHTVLSSSPSLKSILKSPRTSGNSGNDDIIEIETSLDGDNDPKEYIKRVNKQVQFVTEKNKTVSIPPNPPKETAFSGDIVEKETDLFPFEPIPPPIPNGAKQHKSSKFKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q008</accession>
    <name>A0A8J4Q008_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="4">PPIase</shortName>
            <ecNumber evidence="4">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_003240</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000099">
        <property type="protein sequence ID" value="KAF2075459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q008"/>
    <dbReference type="OrthoDB" id="193499at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000002">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0697">Rotamase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="6">
        <location>
            <begin position="52"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="218" mass="23522" checksum="600CA3C61FA34381" modified="2022-05-25" version="1">MNSQSSGQSQTQQFKLTTMQKGFLIIFAILLVTFFAFPSDAKQGPKISHKVFFDIEIDGQAAGRIVFGLYGKTVPKTVENFRALCTGEKGTGASGKPLHYKGSKFHRIIPNFMIQGGDFTRGDGTGGESIYGKKFEDENFKIKHSQPGLLSMANAGPNTNGSQFFITTVVTSWLDGRHTVFGEVLEGMDLVSKIEKIGSGSGAPSKVVTIADSGELKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q075</accession>
    <name>A0A8J4Q075_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Myb transcription factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_003153</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000095">
        <property type="protein sequence ID" value="KAF2075567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q075"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019185">
        <property type="term" value="C:snRNA-activating protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042796">
        <property type="term" value="P:snRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000010">
        <property type="entry name" value="Transcriptional activator Myb isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000016">
        <property type="entry name" value="Transcriptional activator Myb isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051575">
        <property type="entry name" value="Myb-like_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46621">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46621:SF1">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="142"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="142"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="9">
        <location>
            <begin position="145"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="194"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="194"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="246"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="250"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="300"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="454"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="579"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="712"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="751"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="828"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="16"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="71"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="136"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="306"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="332"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="360"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="391"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="409"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="420"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="466"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="585"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="714"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="751"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="774"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="828"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="881"/>
            <end position="901"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2075567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="913" mass="101433" checksum="490579A7776C3CF3" modified="2022-05-25" version="1">MSVDNNRQPLLPLQKGGSGGGGSSGSNGGGQQPIVSLTIPSPTTTISTYPVVDDSNSNAFQQQQPFDENDNSSNNNNSSISSNRMENNIFPNETNDSNTLFNTPTTTTTTTTNSTITNCYSPIPNEIQQQEPDSPKSNNNNGNSKTKGKWTQEEDDILIKAVAQHNKKNWKKIAEHFPDRTDVQCHHRYQKVLHPNLVKGSWSKEEDDKVRELVALFGPKKWSEIAAQLKGRMGKQCRERWHNHLNPNIKKDAWTDEEDQIIRAQHAIHGNKWAEIAKHLPGRTDNAIKNHWNSSMKRLLEKEQHSPSLNSSSSSIGSNNSTVKKQSKKKSTTTTTTTTSSSKKRKNQVNTTTGVEDDNSNINFNSSNNISNNIPIQTQPAHGEDQFDYSINNNNNNNNNNNNINNNNLDVNSFQQYMSGNNNNNTGYSNNNGKNQQNAPVEISNIVTPVKLFSTPGPLKKKSKQDPSTPNRNIHNESFSSDFFAQFPEISFSPIPKNHDNRGSSFLDTSDLSPFKTIYNPNNFFDTPHKHTNLESNFQCFDNFMSPSKLNIVSPVKANYQQPQQSSPLKQLSSYYNNQPMTPTSNGIMNNNNNGNGNGNSGSNILTPTKNQNCNQQQQQNINNNNNVTSNFIKSKILGFQLSDTKGIDKTSLSNINAKLKNPSIAVNVNNNNNNSSKLINNNSINNNSNNITTSPTHVPFSPKIDFLNLPHTPGSTKSKSRMSPTKNNNNNYNNNINYYNYDHSNYILDSNNTSNNTTNGFHGMQYNHHHNNNNDNNNNTNTPQSSQKVPQFDCSLIALKLLKDSSTKSIYSQAKKILSMNNIAYSSGSNGSSATSTPSSSFVISPTQQQPQQNHQTSTSPFLSPSVVPTYHHHHNEKVNGTSSIGNNNNNNNTTTNNSNQFSKSPIPFYLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0E4</accession>
    <name>A0A8J4Q0E4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Pleckstrin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_002286</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000061">
        <property type="protein sequence ID" value="KAF2076429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0E4"/>
    <dbReference type="OrthoDB" id="10266696at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08204">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00821">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000009">
        <property type="entry name" value="stromal membrane-associated protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045258">
        <property type="entry name" value="ACAP1/2/3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23180:SF414">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23180">
        <property type="entry name" value="CENTAURIN/ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="7">
        <location>
            <begin position="192"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="7">
        <location>
            <begin position="407"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Arf-GAP" evidence="8">
        <location>
            <begin position="554"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="330"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="670"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="19"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="129"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="335"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="371"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="381"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2076429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="696" mass="76608" checksum="7C0B86975319AE9C" modified="2022-05-25" version="1">MPPPPITLTPPPPPMPSHGSGSNLNSSPFFPSKNNPQQQEFLESIKAGINKRNSLGNPLYQSPIQSSSSGSSNINNNLSPDTLEKKPSPLSSSISISVSPPPSPPPQLRIEKEKSSKKDKSKKDKEKSSPSSSFLSLSSSSNSEKQSSSSSPISIKSDDLQGALKNLRKTVIVTKKTSLVVESTDEGISMEAPEKRGYLYIMNHETPYTSPIDSNWTQKWCVLKEGCLFVYKSPKDEFESEILSLDHGLVKEKEIKDNCFQLSASLIYHNNEYYDNGPTVTRYLATPHSEEFEYFAWIVSLRGVIFFLRSLNSSSTASLSPSPVNKVKKQTQRYSLPPLSSSPLASSSPTTSISSFVPSHKKVSSKDQHRLSPSTPLSPINSKTSNNSTTVTLSTNNSSAVVEDEFNGFKKGFLSVQGGKLISKWKSRWVVLNQDSLSIYKSQDAEAKKDVRKSISIIFCSAKVIKSTNDKYTFQVVSTDKTIHFACINGSQMLSWITSIQAAQSISMEAYLKYKTGASKSSGGSALNKSTGSLVKLKDDQVESDVQKMLDQNKESLNRLLSQSDNQFCADCHAPDPLWASINLGVFICINCSGVHRNLGVHLSKVRSVTMDIWDGNVIKYFEETGGNDKVNSVWEFNIPPHITKLTPDVSMDVRDKYIRNKYEHKLFYSPSSSTSTSENNSPLSSSTNPDTSISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q182</accession>
    <name>A0A8J4Q182_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001363</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000031">
        <property type="protein sequence ID" value="KAF2077360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q182"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="806" mass="96107" checksum="77E9CE9802719F1D" modified="2022-05-25" version="1">MDSSNNNNSTFFFIYRNHYIRNKIFTHLGLKRNGYYFYRNGLEYHSTEDLQLVELLNTGNLNLIQYRFKQFAQVFISDDEIDKYEIFSQHKTYYEDWLDLPYLTSFNIDRYQQFYYKNQDQQLAAIFDEVFNLFPFQLQVTLPGHVSMNLGISMTRVKLFDRLIPENTYSIHSLFLDDQIFPFLLERKRIYLGKNPERRNYKPLFTNSHFIKHKDLCRTNINDWSDQEFTIQLLEVLEDDCQKEIIDLFKWILQVCPLFAKPKKYPIKHYSKLYGVEVIAPLEKSFFLENIQFAFKTVELLNFYIRHYKSALLSKPEYEINNLQVAQTLLKYCSDPKDLFFAKKKKMMIKTNSKDLNLVVLLTESKVQFYGDLFYENKEIVAYLEKSNRKMLDNHFKVFRDETMTLDDKVLLFYQLLQDRYRPDVFSIYSPLIKDPFPFLIKLASHTTYEYTFFYTKAIREGNIDFVKFCTTRFQAIPHDDDIVSAYEHDHMEIFEFLILRKENFTPIQDEVYCKLLKFACKRLDVKNVIRFIESNIDNHELTRKKVKEQFLNAYIFLAGAKDEDEKKNAIEIVNYFKALFIQYNEPSLEPKHLPSVSSNLFIPTNSSYRPPFFYISFQDFDFFSSHKISFYSRPPITSISALQEALYRGSLSCAKLVLSKLSQQDLSTLQNRIQGILLAFLSSRNFCKSLKVFLYLVSMFSNDLNEKVFLKTASVYANQNENYYLIDILLHRTKIRLIPPKSKEHDREMLEFCYLNLTNSKVKPCDYFKHLPFKLVNINPKLLETHDSKFTKSFAETEHFPSYYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1L3</accession>
    <name>A0A8J4Q1L3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TPR repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001604</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000039">
        <property type="protein sequence ID" value="KAF2077095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1L3"/>
    <dbReference type="OrthoDB" id="10268002at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="331"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="81"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="93"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="614" mass="71948" checksum="E327138D321A3B61" modified="2022-05-25" version="1">MSYIQRISKFKYLNRLLTSSSSSSASPSIYNIVTNNSNNNNNYHNNTNINNDKKIKINNNNIKSITFNNGNRYLKQYYSSSSSSLNSNYNNKQQKEEKQEEKQRDSKYNLIMLDAFENFKNKDIIKCIESIFQGIVLCNDIKLDAYLLRADIKSEFNRTYRATCERQGIIFDYRIVSEEIDNDKKAYIHYSASKAISKLNVPERDSSEAISLLDKALALDPNNLLYLKDKILIGSNLNDPSIISETSTQLLKEDPDKYGKLSLAIIKSMKREDDQAIEILSSIIDENKDLVVIDKDQDSTCYFNRLIMKEDVNGYLRVREKYYFQSENILFDSYLILGNLYCRQGQYELCCSTFRKALKLQPWAYQLHSIMGSLAYRLGKYRECIQHCNDFFAVDQDFESPYAKQSLMDRGSSNYYLSNKLEAIDDLSISLERPWFFDNSRDISNQRANPSLLLIDCYSYFIQLCPGLDKSKFNIDQLIRDTDNDQILFKEPSNIHELYSHVEVFFKVYSDIYDWVGAHPLDYSIEEKNIITTRTSLFSNMKEYLVDSLYLNYISNPHEEFYDPDRPGPYKNFNHFLYLLNYKESNQLESRQQGHITLLNLYKNLLHFINKNDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1R0</accession>
    <name>A0A8J4Q1R0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Saposin B-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_005934</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000251">
        <property type="protein sequence ID" value="KAF2072746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1R0"/>
    <dbReference type="OrthoDB" id="69496at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.225.10">
        <property type="entry name" value="Saposin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008138">
        <property type="entry name" value="SapB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011001">
        <property type="entry name" value="Saposin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008139">
        <property type="entry name" value="SaposinB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051428">
        <property type="entry name" value="Sphingo_Act-Surfact_Prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11480:SF89">
        <property type="entry name" value="SAPOSIN B-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11480">
        <property type="entry name" value="SAPOSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03489">
        <property type="entry name" value="SapB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00741">
        <property type="entry name" value="SapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47862">
        <property type="entry name" value="Saposin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50015">
        <property type="entry name" value="SAP_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035285890" description="Saposin B-type domain-containing protein" evidence="3">
        <location>
            <begin position="23"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="4">
        <location>
            <begin position="24"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2072746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="165" mass="18257" checksum="632BB137BD4C1AAC" modified="2022-05-25" version="1">MKILSIVAILCALVCIFNSVNAFTPIECDLCQLSVDSLESLTKADNLTYSEIYSALGRICTSIPSNYSTICKEFLDLSAPVIINQILNETSSLTICGEIQICSSSTLAKQQKTNQHTLCHYKRISKLIQNNLDCDDSNIACIEFNNYIDHAKNVVLSHKNIKVSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1S2</accession>
    <name>A0A8J4Q1S2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="22">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">CYY_000352</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="26">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17 22">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38567"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:38568"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 21">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 22">Membrane</location>
            <topology evidence="2 22">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 22">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="22"/>
    <dbReference type="EMBL" id="AJWJ01000006">
        <property type="protein sequence ID" value="KAF2078368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1S2"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000021">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000035">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000010">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF150">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 20 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 21 22" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 20 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 22" id="KW-1278">Translocase</keyword>
    <keyword evidence="8 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="358"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="404"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="973"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1023"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1059"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1087"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1126"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="24">
        <location>
            <begin position="99"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="25">
        <location>
            <begin position="909"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1171"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="19">
        <location>
            <position position="472"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="472"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="472"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="473"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="474"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="474"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="588"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="629"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="652"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="685"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="765"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="766"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="767"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="857"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="863"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="883"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="886"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="887"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="887"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="KAF2078368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1210" mass="136840" checksum="D9C44B94197085A0" modified="2022-05-25" version="1">MSLLQESINSTGRRNSISSNSSGSPFLQNSTSSATSPYLHNSTRMNPHKPHTEIPLKELKSSNKLLIEEDPDQSNKPFFQKLWDKIVLNDYFIGDGRTIHINNNENNKIFGYSNNYVRTSKYTLLTFLPLNLFEQFCRLANIYFLIISALQLIPNISPTGKYTTLGPLLVVLAITGLKEAYEDFRRHRQDRFVNESLALVLKGNKFELTKWKDIQVGDIVKVLNKQYLPTDLVILSSSEPQSMCYIETGNLDGETNLKIRQGLEETCHLVDEESLSTFNGLIECEHPNNKIYSFSGSLHMDSKILSLSVKQVLLRGTMLRNTKWITGVTIFTGRDTKLMRNSNVTPLKRSGIEKSTNFYIIFIFFLQILLCAGCAIANGIWNGNHRTTEPSYIYWIRTSSEEGALSFLTFLILFNNLIPISLYVSMEFVKLIQALFINSDIEMYHKETDTPALARTSNLNEELGQIQYIFTDKTGTLTQNKMVFKKCSIAGVSYGTGVTEATMGAMIRQGKSLEDIPIPSPSSNGSGSGANGSTPFVDFNDDRLIADLGNSNNSEQIRDFMFILSVCHTVIPEVEEDKLIYQASSPDECALVNAARYFGFTFTRRTPRAVFIEFQGQEYKYDILNVLEFNSTRKRMSVIVRTPSGKLLLYCKGADSVIFERLSPGQPYADVTINHLQDFATEGLRTLCIATTELDEQIYQEWNKEFDIANTCLVDRELQLERVAETIERNLFLIGATAIEDKLQEGVPETIDTLLTAGIKIWVLTGDKQETAINIGFSCQLLNQQMELLVINEQTKENTIIELNRRLNELSTKEYEETRDNLALIIDGTTLNFALDGNIKISLLKLAKQCRAVICCRVSPLQKSKIVSLVKDSLNVVTLAIGDGANDVSMIQAAHVGIGISGEEGLQAARSSDYAIAQFRFLSRLLLVHGRYSYRRISRLICYCFYKNICLYITQFWFTILNGWSGQTLYERFTLTAYNIAWTLVPIIALGALDKDVNESVVLEHPQLYQSGPRRDHFNIRVFWGWVANGVYHSFILFAFPTLIFRAGLPFTAGKAIDLFSIGTVAFSCIVLTVNIKLALETRYWTWINHVAIWGSIVIYFMWLVIFGKFWEVDSLDVGSDLYSIIYHLGTSAVFYFSILIVPVIALWRDFTWKFIHRSELTKPYHVAQELQKSSTPKKSIGSKKKSGYTGYSFSQDSGQADALKKFTES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1S5</accession>
    <name>A0A8J4Q1S5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002316</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000063">
        <property type="protein sequence ID" value="KAF2076359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1S5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="705" mass="82640" checksum="1048342614819C0A" modified="2022-05-25" version="1">MIQQQTEYFFSIVRNKWIFNQILDLCKYQDRISYNFYDLPLALICKLKRFDIFDKKWHQKSLLQSPHMIDPKYMMDLDVKDIFEFFANNTSLKRFLMVWETMKPLVDNFDKMQKIIISSAQVKNIEEGIVETLLEFMSKTTIIYSNIKNAIYFGGYGSMKIINSFLGVADRTSGLCSITFLKQFLIGASKNGKKHTCIQLIHQIMERGFTEFDISDLSIEINDYLLGLSENVSIRLVVSSLPPQNLETIPWILAHRDKLIINSRCTSFLMNERIYLENKTFFEFMINKFKECYPNDRSPFLNGIPSIPNENCIETLEYILNVKDFFTFSSASLNSPFKLVDLIIKRHPRRIPFIEKEMKDWYFNDIRIVRLIDQVYNSPFTIVSLDGCVSMGYKEIVIFLLSKNNITINEKTMTAALVFPDIYNLLLPFVPKDINLGKIFESKSQVILNNLIVKGSLDGIKLFAEQCHIDPLSNPLYKVFNYSFSKTVSIVPKIYKYLLEPIENKLLDQATSTYSLNNLIHNSLNNRNYNLARYIHSIADMGDYFFVNFNITLNNQKIIYEITKSMSNSVLLKIFREKKEFSLLYSRMASFVTTTLESDQEEMKRKQSMYAFILNFVCIAIDLKLVLQLDQFAQFKLPIKFTYQLFSSFLPNDHIRPFLPNLKFLNQLKKETIDYLNDTNLIAIFTNPTISDLNDYYHNHLFEFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1T4</accession>
    <name>A0A8J4Q1T4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA polymerase delta small subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002331</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase delta/II small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000063">
        <property type="protein sequence ID" value="KAF2076374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1T4"/>
    <dbReference type="OrthoDB" id="3763at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902969">
        <property type="term" value="P:mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07387">
        <property type="entry name" value="MPP_PolD2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.50:FF:000002">
        <property type="entry name" value="DNA polymerase delta small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040663">
        <property type="entry name" value="DNA_pol_D_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024826">
        <property type="entry name" value="DNA_pol_delta/II_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041863">
        <property type="entry name" value="PolD2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416:SF0">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18018">
        <property type="entry name" value="DNA_pol_D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA polymerase delta subunit OB-fold" evidence="6">
        <location>
            <begin position="88"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="5">
        <location>
            <begin position="239"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2076374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="508" mass="57797" checksum="B93F658F0421635B" modified="2022-05-25" version="1">MIKNNDNDKNYTFDDNESLQDLFKTISRIERDEVNYIDNDGNDQDDLEMMVEKEYQTLKRVQVPYTFHKNRFIDQPKTNNTIVASSLQYNTVYASRLEKLRPILLQQVEQKYSEITIQKILHLKPNDECVMIGTLYKEMDLKQNILKQYAQDRAILPPPAQRDSFIGEGDKLFFEDETGRVKLIADKQVVDSLLTGMNVAIKGKGLLGAEFEIEEVFYPGLPPQPHSKDLENLEDDVYVCLVSGLSIGDPNNTLQLNMLVDYISGNLGTSKDQKFISRISRLIIAGNSIFKEEQSHQSFMKYLSKQEINSKKQKMAIPIKEFDSILSELAKSIPIDIIPGPNDPTNSSLPQLPLNYCLFPFSVQNTNTNPTSNPYESQIGGRLFLGTAGQNIDNASRYMIIDSKMDLTEKTLNWRHLAPTAPDTLSCSAVDHDPFVIEQCPHVYFIGNQEKYQTKMIRGPNDQIIRIVLLPKFSESNTIVLVNLKTLDTFPIEFNCTTFFPQSPCSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1X2</accession>
    <name>A0A8J4Q1X2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glutaredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002103</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000055">
        <property type="protein sequence ID" value="KAF2076617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1X2"/>
    <dbReference type="OrthoDB" id="415696at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03028">
        <property type="entry name" value="GRX_PICOT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000012">
        <property type="entry name" value="Monothiol glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033658">
        <property type="entry name" value="GRX_PICOT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004480">
        <property type="entry name" value="Monothiol_GRX-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00365">
        <property type="entry name" value="Grx4 family monothiol glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293">
        <property type="entry name" value="GLUTAREDOXIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293:SF73">
        <property type="entry name" value="GLUTAREDOXIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="6"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Glutaredoxin" evidence="6">
        <location>
            <begin position="155"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="120"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2076617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="26986" checksum="D53AEE251FD45F80" modified="2022-05-25" version="1">MAQAINTVEEFENTIKTNNYVVVNFWADWSKPSTQMTQVFNTLAEKSANKIKMINVEAEKLHQVSLKYNIKSVPTYIFIAYQKVSNTMVGANPAELAIQVNSFITKPPQESSSSSSTTNNDAHNEEILKQMKANQEQEKKNLNERLEKLINQAPVMLFMKGHPDKPQCGFSNKSVAILRENGFTFDSFDILSDPAVRNGLKEYSNWPTYPQLYINGKLVGGFDIIKDLNEDGELAELKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q276</accession>
    <name>A0A8J4Q276_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Spt20-like SEP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001293</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000030">
        <property type="protein sequence ID" value="KAF2077365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q276"/>
    <dbReference type="OrthoDB" id="1932706at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046468">
        <property type="entry name" value="Spt20-like_SEP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12090">
        <property type="entry name" value="Spt20_SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spt20-like SEP" evidence="2">
        <location>
            <begin position="196"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="532"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="601"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="828"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1024"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="298"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="601"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="828"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1024"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1050" mass="118942" checksum="B1B3120508A2BE6D" modified="2022-05-25" version="1" fragment="single">YLSYSHYQPVCTNLKYIQHYMNKQNELQQHIFQSNPLSTTTSALSSTADIGHTTIDTSVPSTTTTTTDTSSSSSTTTTTTTAMEVEMDRANSKYQPNLSQPIHSENSPQFTNDTAASSSSNSMQIDNPTTIGITPNGNHINNNNNNNSIKESSNNNKLTLKRKKELIKLMGLESNNDDDDENLTIKPTTNPIYNLKPSLFIHLYPTIGYTLDNINKNKVYSYNEKNSKEFLNSISQGRLLPDLIQILDEIKSSNYYQGCIVVDIKDYRGFLSSENALTNIQQQQQQQQQKINGRSELSTSTSSTSSKSKSKSNHNNNNNNNSNNNNSNNERNFTSEQLQLFKEYIEKRVLLKPDMESVLLDIKNRISLNQDLILNDYIKQQEKQEKEEEKQENLDPMDNIFDETMLEFEEKYLLAIEPSLELDPDLDVFFNNLETNQNQQYQQRLLNFKRLKTIHHEPLSPYQQQQQVQYQVNQNCFNELPFSKKKTNYFNNMPAKIPNSLKHFNPYTAPSLLSTINPQNLSPETLDSISINNNNLDNNNNKSRNIDNSNNNNSDKNDFLLFKLLGSNQKWDNERRSTLKFYNTDFKDFKSYPYSFQNNNNNNNNIFVNNSSNDNVNNNNNNNNNKKQPEPPFKNGVLPTKQISHDQLKDRVIQYYSTSNLTVLTYEINLKSGSYELLQYITEHPTFYQTIVNLKNLRSHPQYNTNPQYLPMVQKFLSNLNKNVATLTQTLNPELSFIYDTPLPQPLYNSSMIGSYSNALFFLNHLKHSLENSGTYVLVYDSISPKVISTQAVETRKRIEQKLLALNPSLSQTAILISVPAAKPAAATTTTTTSTNIPPSTSSSSASSSAPTTASTTTTTTTTNTNNQTQSITTTTTIQPPTLINSIPLNSNTITTPTTVAPPLIQPTLAPSQIVNPQPTMINPSQSPQQFLELIKSKKMTLSNHMQQGQQQYAMYQKNLALYEQQNPQPVAQIQQTKSIMGNISNQLLVLKNQFQQLLTQEQQILQQLNNNNLGTTTTNINNNNNNTTPMNSTLNAQPLRVINPPTIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2C9</accession>
    <name>A0A8J4Q2C9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_000156</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000003">
        <property type="protein sequence ID" value="KAF2078531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2C9"/>
    <dbReference type="OrthoDB" id="19515at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17502">
        <property type="entry name" value="MFS_Azr1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1720.10:FF:000004">
        <property type="entry name" value="EmrB/QacA family drug resistance transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004638">
        <property type="entry name" value="EmrB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00711">
        <property type="entry name" value="efflux_EmrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF197">
        <property type="entry name" value="COMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01036">
        <property type="entry name" value="TCRTETB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="205"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="306"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="331"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="361"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="397"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="425"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="467"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="508"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="533"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="562"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="594"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="668"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="208"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="147"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="700"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="760"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="44"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="71"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="158"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="705"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2078531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="800" mass="87577" checksum="8B92C3FBBD8B0476" modified="2022-05-25" version="1">MGQELNIGGNDSTIYVGQEDKQQEQQLNSNNTSIDCISTAIEDQYNTKGPSENITPPTISPFKKMTTDSQQDNDEDEDDQESYEVVDLNDDHVKDKNHNDSQFVQKKPVISHKKIFNLQQKNGSSISSFDSLDKSLELPIIELLSQQDSTNEEGFPATPNSMAASNQSNMDINSSVDSVSSPESSSANSSRTNTFVQEELSQKKILIIFSGLMMSLFLCSLDMTIVATALPAIVNDMGSLEQLSWIVTIYLLTSTSVSPLFGKFSDIFGRKTMLLTSLIIFCIGSLLCAVSTTIDMLIVSRGVQGIGGGGLMSAVMIVMAEIVPLRQRGKYQGLLGAVYAVSSVVGPLMGGTFTDNVNWRWAFWINLPICAIAFAIVFFALKIPQEVIPFREGIKRIDIIGTTSLVSAVVSFLLALSWGGQDYQWSSPVIICLFVGTAIFVGIFIINEKFFAAYPVIPLGLFTNRNYIICSISSFLLGFIMFGVIYYIPLYFQMVKSESATYSGLQLLPTMLGIVIFGCISGLLITKFGHYKTYPIVGMLMMTIGIFLLSLWNDKSTQSHYIGYQCIIGIGIGLTMQILVLIVQNSVDYKFISISTATISFFRTIGGVVSVAVFSAILNQQFQNNLNDLVAKDSMILNGLTASDFHAKELPTFEGYGRTDVVGAYSRALSIVFLSATPFAALGCVLTLFIQPLKLRTTLGPQKPSISTTNNESNENTIITNDGVDNNAQNNDAAADGATATEKNNIEMLEIVTVIDENPSKSENEEIYNSSNNNQTQPTHQLNQPIYNSHKCIEIEPIGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2N9</accession>
    <name>A0A8J4Q2N9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_001049</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sorting nexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000023">
        <property type="protein sequence ID" value="KAF2077662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2N9"/>
    <dbReference type="OrthoDB" id="271164at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45949">
        <property type="entry name" value="SORTING NEXIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45949:SF2">
        <property type="entry name" value="SORTING NEXIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="9">
        <location>
            <begin position="503"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="270"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="433"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="480"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="71"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="116"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="275"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="299"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="437"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="490"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2077662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1083" mass="126036" checksum="96BF67373D0FFD2E" modified="2022-05-25" version="1">MSRQQQQNSNNRFYFGDNNSDHDSNDDSQEYNNSSANNSGNNSVNGGHFHDSSDGDDDAVECISVVDNSHHSNNSSNNSKSIKNITNQMGNTTLNNNNDANSNSNNTNAGIRTSINKNPVNNNSNSNNRNNNNNSKNNHNNQYDSDEEQEPQEMDDQVDGDNEEPQKDFSWLMKNEFMVHRKPGIFHRANMGELIITTHRVVWISDSNNDDKVLIDINKIFHTLAASSQIQQTWKLKTSAADYLFIFPEPYTKQSVGEVFGHYQNSKKNLTAKNQQQPLSSSPPLESFDNKPNQQQKVQQREQQQREQQQREQQQREQLKEQQLREQKQQQQREQQLRDEQLREQMIDQKIREEQYKIEEEKRQQEQMRLNKKQQQQQQQQQQLQQQQQQQQQQQQQQQQQQYQQQQYQQPQQQYHQQRYMDDEFEKEEFEGYPDINRSNNNNNNNNSNNHNSNRNKQSSPTNSLIKKKENYDEGFVSVSLNSVGDEGDSNWQEESNGIDQDGYFDLEVRDPRPHSDDAGVYITYEIIGKYKDSKNPIPVNITARRRYREFEWLHSQLTLPSNHHVKIPSLPQKSIYQFWNKTDPTSTRQRCSALDHFMKSLSVSALLRNHPALKMFLSGKVISMSPTTITEVIGNALLSPNLVGINFNGPNDYNLLDGNFAYESSIERDYWISRKLMSKWKRIPKRLPFEKFLVVVLGTVSAGKSSFINNFFNLQVKVSADEQLDTHFTLIETIPRDQFYQLSDQPAPKRFSPDQLKQRIRGEAVSDPRHGHVFLYLDKTFTLSRYDAQFSNYRDIISNLNLVRTVLINEEYLTGAPEEIQNQKKTIFIDSPGFEPKTEHDPEKLLGSIRVVQFFQSTSDLTLFMMKAKELALVSSQIQIVELCVLYKSYGPEVVDHMVMQMFSKKAESKSFFSIFEMVKGLVEQIRLKNIPKTQESNGTSVWDGIKFVLTQIDTVNSNLKEQFFELGRLLGKGLMFLDPPTFRQCKAIALPLDRQFRDEQDKKQKCGDLDELLQYIYNLNRGSTYNSRLEGAIEEMTTDLLSNIQKSWTNYLFNTDYNLVLQLKDRSKTRVSGPSHSYRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2S1</accession>
    <name>A0A8J4Q2S1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">5-demethoxyubiquinone hydroxylase, mitochondrial</fullName>
            <shortName evidence="8">DMQ hydroxylase</shortName>
            <ecNumber evidence="8">1.14.99.60</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Ubiquinone biosynthesis monooxygenase COQ7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">coq7</name>
        <name evidence="10" type="ORF">CYY_001053</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the hydroxylation of 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) during ubiquinone biosynthesis. Has also a structural role in the COQ enzyme complex, stabilizing other COQ polypeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 5-methoxy-2-methyl-3-(all-trans-polyprenyl)benzene-1,4-diol + AH2 + O2 = a 3-demethylubiquinol + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:50908"/>
            <dbReference type="Rhea" id="RHEA-COMP:10859"/>
            <dbReference type="Rhea" id="RHEA-COMP:10914"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:84167"/>
            <dbReference type="ChEBI" id="CHEBI:84422"/>
            <dbReference type="EC" id="1.14.99.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="8">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of a multi-subunit COQ enzyme complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion inner membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the COQ7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.60" evidence="8"/>
    <dbReference type="EMBL" id="AJWJ01000023">
        <property type="protein sequence ID" value="KAF2077665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2S1"/>
    <dbReference type="OrthoDB" id="275371at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008682">
        <property type="term" value="F:3-demethoxyubiquinol 3-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016709">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01042">
        <property type="entry name" value="DMQH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01658">
        <property type="entry name" value="COQ7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011566">
        <property type="entry name" value="Ubq_synth_Coq7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11237:SF4">
        <property type="entry name" value="5-DEMETHOXYUBIQUINONE HYDROXYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11237">
        <property type="entry name" value="COENZYME Q10 BIOSYNTHESIS PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03232">
        <property type="entry name" value="COQ7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2077665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="228" mass="25691" checksum="D21DBA9F53601E0B" modified="2022-05-25" version="1">MASLQKVMRFGSRSGSYLIGSSTTTSSNKYFTSTTTTANNSNTSKTSQVDLEKERLKRELIERIIRVDHAGEFGAARIYEGQLAVLKNTKEGPLIREMADQELEHQAKFNQLIYEKRVRPTVLSPIWNVAGFGLGYVSALMGKEAAMAVTVAVETVISEHYNDQLRQLNDHQIDDKDLKDTIKKFRDDEMEHMHIGIEHDAELAPLYKPFSELVKVGTKAAIWLSSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q372</accession>
    <name>A0A8J4Q372_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_001373</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000032">
        <property type="protein sequence ID" value="KAF2077307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q372"/>
    <dbReference type="OrthoDB" id="5817230at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060176">
        <property type="term" value="P:regulation of aggregation involved in sorocarp development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000563">
        <property type="entry name" value="Guanine nucleotide-binding protein alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000007">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF180">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-4 SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="41"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="146"/>
            <end position="147"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="196"/>
            <end position="200"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="265"/>
            <end position="268"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2077307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="345" mass="40157" checksum="D7BF0CF1AB31B963" modified="2022-05-25" version="1">MRFKCFGSEETEASSKIDKIIETDRRKLRKDVKLLLLGPGESGKSTIFKQMKIIQEDGGYTVEELLEYRAFVYSNCISQMEALLTASAKLNIELEVENKQRAANVLRRTIGNEPWLLLAADIKHLWEDKGIKETYSQKDKHFQLNDSAAYFFDNIDRYMKEDFIPNEQDVFRCRVRTTGIQESEFTFDKIRLKIVDVGGQRSQRRKWIHCFDCVTAVIFVAAMSDYDQVLREDESVNRTRESLALFKEIVNCDYFKETPIVLFLNKKDLFKEKLKRVPLQSCFSDYTGPNKYKDAAMFIQSQYLAQGPSPRTIYTHATCAVDTENIKFVFRAVRQTILSQALEHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4G6</accession>
    <name>A0A8J4Q4G6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000002">
        <property type="protein sequence ID" value="KAF2078625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4G6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035280043" evidence="1">
        <location>
            <begin position="20"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="150" mass="17103" checksum="46A281699977103B" modified="2022-05-25" version="1">MKLIIALCILLLSIQSCYGAGVLNRMSSLHIKARISWYSEDKDSSGFSGDLFSIPPGSQEAFRRDLTDEGYAVYVEINPTPNRVGIQDRYTNTYLVPKEANIFFYDDHIIIDGAGRRHIIPWKYEKGKNHEHYMRNSDPICEGYAGSSEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4M5</accession>
    <name>A0A8J4Q4M5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PBZ-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000512</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000009">
        <property type="protein sequence ID" value="KAF2078222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4M5"/>
    <dbReference type="OrthoDB" id="10256774at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22671">
        <property type="entry name" value="FHA_APTX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039253">
        <property type="entry name" value="APLF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019406">
        <property type="entry name" value="APLF_PBZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21315:SF2">
        <property type="entry name" value="APRATAXIN AND PNK-LIKE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21315">
        <property type="entry name" value="APRATAXIN AND PNK-LIKE FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10283">
        <property type="entry name" value="zf-CCHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PBZ-type" evidence="2">
        <location>
            <begin position="159"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="119"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="208"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="275"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="537" mass="61602" checksum="F9F73B78EDFE70C3" modified="2022-05-25" version="1">MSSEPKDILRVVGVNHDGTFKDYENVYDQFGSSFIGRGDFDIQEKKCSRKQISIIRNNDNSNFIACNGVNPSYLKKHDQEFFVQMVRDQNYPIENGDCFSMIYESFTFQIIIESKKNKQQQSNSNNSDNENNTTTDKKRKAIDSDEKVEEKKKKKDELPVCPHGASCYRKNPTHFQEYSHPWNEKKSVSDDDQETKNPKDLKPDLNKTSTTTTPKTITDNVPIKQPDLQIQQKKPQPQPQPPQPNNDQQPQPQIKKDIPKKLTLQDIKKDEPKKTIPTTTTSSKNDITIPNPIPTPNPLVAASNNDKAQQQSEEISNAGKIVGPKYKKKKLSDVHKPRTLVFPFLSSIKCGIDIDKAAEIASQAIQEYLQFHQNEEDIKLKMVIEKTIYSDALESFKKILGNKWDKRFEILSIENLNSIEQFSNNCRIYATESTWRLKKIPSNKFLYEDMLSIDGKAINFEKDTKNRFPNPAKIGKSYPVAVNVETRLHKEYGVEYVVLILPPNMNPLKPDCLASYTQSLPLLKESYHSLFTVLDNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4N0</accession>
    <name>A0A8J4Q4N0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004816</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000178">
        <property type="protein sequence ID" value="KAF2073856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4N0"/>
    <dbReference type="OrthoDB" id="20554at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900034">
        <property type="term" value="P:regulation of cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039313">
        <property type="entry name" value="HIT4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33704">
        <property type="entry name" value="PROTEIN HEAT INTOLERANT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33704:SF1">
        <property type="entry name" value="PROTEIN HEAT INTOLERANT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="399"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="427"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="466"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="478"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="508" mass="58307" checksum="8EA7924974C2C4BC" modified="2022-05-25" version="1">MDTPAPDVKSKRVVKTAAERAQEKADSLKKTLRSVDWRDLTPIGFEFGTWESMKKEKWEFENLEHELQEGVLSDEKHPYYMFMGAQPIVENKVAYNMPYIVVIDCQTPPPSTVCKASIQGGGEEIFPMEQFHLKWSPYIRSKYVSLNVGARHINYPLITLNLQERAGKRVSEEKAKNLEYLLPYIRLPQISEKLTKQEVKSVSFPYKAKLSEEKAKELLKARKDEKDTYDKAYEKWKSEPKSKKVHAPVKPGKEEPCIKGEDGAYYKVIEMTFDKSVDTLTDFIPEFIDDNGLDAKEGDNIKAALKKAFEEKRKVVDEKFNQMLAEVAAYSNEQREDYLNIKVYKFYPTHPVFKVSQYIDPMVNRFFGKATETFPKPSNKVDLSKLEQLDEKTLRKIEEDDAKKEEEAKKKREAAAAKAKKETKDVEMEEAESEEEEEKKAESESEEEEEEEEKKLKATRKPTAAKGKKSAKSTKVSKTKEAEEPKAKGKSKSSASKKVANAKKTKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q5T0</accession>
    <name>A0A8J4Q5T0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_004216</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peroxiredoxin family. Prx6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000145">
        <property type="protein sequence ID" value="KAF2074471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q5T0"/>
    <dbReference type="OrthoDB" id="2996783at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03016">
        <property type="entry name" value="PRX_1cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1020.10:FF:000001">
        <property type="entry name" value="1-Cys peroxiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000011">
        <property type="entry name" value="Peroxiredoxin PRX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1020.10">
        <property type="entry name" value="Antioxidant, Horf6, Chain A, domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050217">
        <property type="entry name" value="Peroxiredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045020">
        <property type="entry name" value="PRX_1cys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681">
        <property type="entry name" value="THIOREDOXIN PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681:SF128">
        <property type="entry name" value="THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 7" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="5 7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="9">
        <location>
            <begin position="39"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity" evidence="8">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2074471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="249" mass="27659" checksum="3F07740C3B68661C" modified="2022-05-25" version="1">MYNSNKLINIASNANKYIFRAYCTGTPSAASSSSTGGNLRIGDVVPDFSQESSMGNINLYKYLGDSWGILFSHPKDFTPVCTTELGRVAKLKPEFEKRNTKVIALSVDSGESHKEWIKDIQETQNVPSIDYPIIADADKKVANLYGMIHPNAANNMTIRSVYFIGPEKKLRAQITLPASTGRSFVEILRIIDSLQLTDKHKVATPAEWKDGDDCVIIPTISDQEAKGLFPKGWKALKSYLRMTPQPNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q9I3</accession>
    <name>A0A8J4Q9I3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001294</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000030">
        <property type="protein sequence ID" value="KAF2077366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q9I3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="744" mass="86339" checksum="D8B5E28F421A8CAB" modified="2022-05-25" version="1">MMINSNHLTSICRLNSRSVLSLFQSSFILPPSSSFSLVKQHQNLNQRKRFFCTATTTSTTTTTTSNSTISTIKNNDNKIIDNDIEKLIQNAKTNSNNLESFLAFSNIIKELGSNINSKNYSLLLESCLGLIYKYQLYLNNAYKKDPHSLLANLKDNTSGGFEFQSPSIITNQSIIDYSQYLLDRMSKLHINSNNNNKNNSNEHPFIHCQTILKLLNDTLIFYNQQQQQQSSNNSNNQSNNNKLIKLHKELKEIENIFDQIQYKYDKEVEKVLELIQTRYHYEATSKAVPLLQHANNSNLLNLLLENLVLYGNSSFGLLILESYKENFHGIEKDQDGNPLPSDEKLLADLIVFSILSKNSAKVGKYAHQLSAIDPTFTQEKLNLLLSHQDEVFYSLNFYTMKNDPRRHIPTLEKLFDNPLDKKDHIIAQLVLCDLYSIEKRFDKLITQHYFRDTLNFMKNHLDHLEKLNGLLSHFQQSYSIEDILYSILEKKKDLFIEKKLLTEGIEYHFELFYLLKYCIPFADPIKMNHLLSNLLNGSAKLLYTKGKYKNSLDVFKKSLKLFAYSNNLPYQMIMWNNLFLQSQQYSQGIKMIKLMNEKSTSFPWMELMTSREKIEYHSLSFQICFKEIESLFRQNRKNLVSICSELSDSGIVSWDSKKMFNFNTSKVSELIHDVVYMEQVVAKLIHELKPADPLVLHDVLSLMSLAIQQFLKFIKELNSTSDTLLEKQRKSLQELGVLVDDLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4USP6</accession>
    <name>A0A8J4USP6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_004821</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000178">
        <property type="protein sequence ID" value="KAF2073861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4USP6"/>
    <dbReference type="OrthoDB" id="427030at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001818">
        <property type="entry name" value="GDNF-inducible zinc finger protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000125">
        <property type="entry name" value="Putative zinc finger protein 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001102">
        <property type="entry name" value="Transcription factor IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051061">
        <property type="entry name" value="Zinc_finger_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179:SF13">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="167"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="195"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="226"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="257"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="290"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="350"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="379"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="48"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="427"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="60"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="106"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="145"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="433"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="453"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2073861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="587" mass="67791" checksum="F3AF224921461084" modified="2022-05-25" version="1">MVVGSENICTDVSGELMLDQMIIPDPHPLSPIPTCLKRNRDEFLKHISDYSLEQEEQDNSGDQSNDSSSSIDNSIVDHNQSNQSVVQQDGDDDESNQYLNLNSEDITQDGEDDDEESHDEEEDESEEEEEEEEEEEEEEEGQESKTTTTTTTTKNKGGKKKYDAWDRTCEYCGKEFSRKSKLVYHLRTHTGERPYVCTFEGCNKSFTRSHHLKYHEASHIPPENKLPCPTIGCTATFVHKHHLLNHIRVQHSQEKPYKCTFSPETCHLSFSKHNQLKRHIASTHMHQLPYECTYEGCDKRFEYPHHLKKHIKARHEGIKTNVCMFPGCTESFYTQLELQKHRKSAHVIMYTCDHCGASFKQSTHLKTHKETHTEDRKRFPCTYENCVKSYATKSGLDAHIKSIHKGELFTCTMPRIKEVQEAEPKNVYISKTKPTKKRRTKKRKVDNSQGKEQEDEEEEEEENNETDICGMTFVNKKNLCRHIEVVHNPSRNGVKIIPSEHSKLLKSQAIKEKNHQERVLKHILGNPNTTKSNSNPIDSNVDVGVVDQQPNVDVNVVSEKSIIEQTMDEIFRSHETELNLMEEPLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UTV1</accession>
    <name>A0A8J4UTV1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Saposin B-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_010410</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001093">
        <property type="protein sequence ID" value="KAF2068263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UTV1"/>
    <dbReference type="OrthoDB" id="16524at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.225.10:FF:000002">
        <property type="entry name" value="prosaposin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.225.10">
        <property type="entry name" value="Saposin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007856">
        <property type="entry name" value="SapB_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008138">
        <property type="entry name" value="SapB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008373">
        <property type="entry name" value="Saposin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011001">
        <property type="entry name" value="Saposin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008139">
        <property type="entry name" value="SaposinB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051428">
        <property type="entry name" value="Sphingo_Act-Surfact_Prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11480:SF3">
        <property type="entry name" value="BCDNA.GH08312"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11480">
        <property type="entry name" value="SAPOSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05184">
        <property type="entry name" value="SapB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03489">
        <property type="entry name" value="SapB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01797">
        <property type="entry name" value="SAPOSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00741">
        <property type="entry name" value="SapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47862">
        <property type="entry name" value="Saposin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50015">
        <property type="entry name" value="SAP_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035311975" description="Saposin B-type domain-containing protein" evidence="7">
        <location>
            <begin position="20"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="8">
        <location>
            <begin position="20"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2068263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="109" mass="12524" checksum="8CE6670817210DC7" modified="2022-05-25" version="1" fragment="single">MKFLFVLFAIMALTWVAQARINKCEVCQVVVHSIEHSLQKNHTADQIVHQLERTCKFLPKKHVASCQSLVEKHTPQIIARIAAKDHSFKICSKLHLCKKAHLREVLESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UV65</accession>
    <name>A0A8J4UV65_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009856</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000832">
        <property type="protein sequence ID" value="KAF2068818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UV65"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="268" mass="31333" checksum="C4C90547AD1248E9" modified="2022-05-25" version="1" fragment="single">KEDKEKEDEEDEEEVQKQKQQIYQQQQQHQQEMQPEEQQETYLQHQLRKAYIDEMVKRIKRERESFAYDFQKFDNFPFVDVPYQDDIFDVFEDSYYLDTGVIDENNQSYCAFKQEGNSVPQIPHYSFLLLIEAFKLLRDHKSLTELSIGNIGCGDDDCGQFDIRNLDVAAVALLKSILVQNKSIKTLTLDFEYVRGHPILSPEFLMSLLKLNTTLEHLIIGNEDPDSTDTDLQTIINFAQAHTKNTKCVISIKKKMLLKSLSGKLMYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVX4</accession>
    <name>A0A8J4UVX4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009543</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000737">
        <property type="protein sequence ID" value="KAF2069135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVX4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035329368" evidence="2">
        <location>
            <begin position="22"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="117" mass="12875" checksum="853C1A2E6FB07CAD" modified="2022-05-25" version="1" fragment="single">MKKGTLILLIFVALFVTLSICNDTPPTNRNNSNNNNNANQNRKDNDIVTAAGKENNSNILGGKDKDHPVKNNSDSDSDSDSDDDDHHHKHCTDLDSDNEFDDVKYKHCAKSGSDSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UW10</accession>
    <name>A0A8J4UW10_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009474</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000718">
        <property type="protein sequence ID" value="KAF2069208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UW10"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="557" mass="64893" checksum="FA2722AC4D1CB2BA" modified="2022-05-25" version="1" fragment="single">RVWNIFYHILRSNREREQEELDIVLFNLLLLENNDNDNDSTADNRYINEFVNDVYKKRRQHTNNYFRPTLYFSEKIGSQGSIALFDASVQVVHQIFNDESIVLKYQYKCLIGASKAQKSSLFHHIFSKMKPLESRVEPMKILFKLEHFSTKQDVLFILENVPLSLILLGQFPYEGMCTDSDFIAAITMSRAKTISFSRAFEMGNIALLQMMLKQVPYFCNLIFTKIPTLEVLSLFYDDIMMSVHPNSRLELAVSHSLDMYKFLHAKGFKFTPCCLDSPYDILVYLHENGILANIFYTDIIITQHYHYDVRVVRLLHQVYGQDFKISKWVLGKLVAHGDTASIHYLFTVYDYCGRAHPSGVECLQLLITASRNAPMFHVVANYVHSYMPQCVVEFGNLTKRGKLPALRGPLEWLKSTLPAHTCLLFQFECDEYKDCTTTFDTHDLRYPAVYKLLLDGFTEGYHTTHVDPVLYLGCVFKFAMRTHNARLLTHICRDLLPSIRNHMFVLSNFSNLYFSYYEKSIIQALPYLDLTSLLEFLTDKPHFSRLYQYVSSIIQIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWN2</accession>
    <name>A0A8J4UWN2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008946</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000604">
        <property type="protein sequence ID" value="KAF2069733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWN2"/>
    <dbReference type="OrthoDB" id="10538240at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="432" mass="50650" checksum="0AE382BA385C53D3" modified="2022-05-25" version="1">MNRSQSLYIYTEHSKEKQENVFIPSKPSSSVQRSIFIANQRIRAHSAPSRFPSLNSSRNNILSHSRNGSGGNISLSKSFSIKNGDQIQQIQQQQTVVLPTLVSESDVFFPDSPLGTPRVRTNTKKVAEITNDLKNLQRPAEFDDQTSTNISIPYNSRPMSEVIAERNGWIVLNSIDRVFLDAKQGLLNIYKTRQHKVFYYKTLKLKNFTLKNKDNEFTLVYNKQQVKEYHFSSCNQGYEKQDIEEWIHALLGMGCISETIVQYQKQKEMDKLEQLKQNEESYIQDERSKKKRSSALLDYQLVPDSLISRLDWSFELHKDVGDNSFSIKVSIDNDRYNWIIKRDIQQLNQIFIIFRKQLWKDQFSKDKSLLKLQYGSLNDLLNNFFDTIETNIADIFDIEENKKLRNYFIKFISPISWYDEKPINYINPFQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UX74</accession>
    <name>A0A8J4UX74_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008563</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000539">
        <property type="protein sequence ID" value="KAF2070123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UX74"/>
    <dbReference type="OrthoDB" id="17015at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053019">
        <property type="entry name" value="GATA_zinc_finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23353:SF32">
        <property type="entry name" value="AAC-RICH MRNA CLONE AAC4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23353">
        <property type="entry name" value="RAB-GAP/TBC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="439"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="481"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="508"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="670"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="703"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="739"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="773"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="819"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="886"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="996"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1024"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="601"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1093" mass="124457" checksum="2C32ECEDB52B449F" modified="2022-05-25" version="1">MEGNNSNGTSTTSTNGNNSSNNNNNSKIKCKFKYVWLLWRHIKKSYIIRQAIVLLQVLVLFYLISQTVIKFQMLDNKTTIKEFNNNECSTLPTSDDCARVGYNFSRVLFFYNDGLARDLSDDLVNMFKSHSNTFRIQNEGFPASYAVFSSYVTGAPPTNFMGNPILSDNIIYQLAHNNITLRYYGTRMPAYDVLEKGQYFDRAEIEYVSNTDSKLYTNLFECGKGAEACSTEFLEKQKSANMSIFFSGDIIDERNHHTGNKLDPVTLETINTWISNMKYIKNWIDANPEYLLVIFSDHGGKLLSETAAHGGNNDGNEAFMIIYNPTLTPLPNEQQDIFIDQVDVCATVWQHFAGAGVSLPIENIGKVYPTTTDPEKIYQTLKLNADQLNRFGHHWGYKMKQSDFTRAIDLHKTDIQESIKLFTKYINSLKIPFLNLKRFPWFEVTMYPILIALLATSLLYRQYGSFTNLGLQLKSHFTLVFIPYLIIIGITGLFSGFWVLYWHDDNESIHMFVASLLSTLAIHYSSFGMNIEFSPTNIINASNSGLDENDNVLNNGNGNVEMDQLRSPLLSVGASITPSLIPANDNDFFLNGNNNNTAYLTSSGGSSGNNSMNNSGNSVNNSHNNSNNNSNNNSPLSSPNSLSSSNTDDLYSISTNQPLSIDYKKPSNWIIIYAFFCFFMAVFLFLLTIPLEDLLLVHLYEKSWLIATFFLVLEYIIQKRRIMAHFSGGEKNNNKPPRGIFYPLYLKFAIYLFILFAVYYYDKQWLTHHVMEKHLAFTIYFILGVHALILLFSNRFNQADIILPISMTLYFLSNNKERFYLTLFVIPQLYAISNVFYYKLFNVLSPIIKQYRQKSLRSIAASKTNYQIFKYSLKNINFFSGITDHFLPFVVLLMTTIALSCYKLMKMNFQIGDVAIYVPGIFDPPTTPVFSGFIMAFHKLGYFFLLGGFLIKLANPCPPAIFPSHYKVGGTKFIFGYKFRSNSDFNRISESLNAQVWGFLLLILLCSLFFFHLGYYNFMVTKAFVFTVTLCVVVMVYGVSLIISAIGEIIKSFMRTTSFCGGKKNNLNLHYSTSSMSSPSVEFHDLKKKINAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UX92</accession>
    <name>A0A8J4UX92_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000006">
        <property type="protein sequence ID" value="KAF2078346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UX92"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040405">
        <property type="entry name" value="DDB_G0275255-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34411">
        <property type="entry name" value="DUF6748 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035269989" evidence="1">
        <location>
            <begin position="20"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="300" mass="34738" checksum="796FF8CB4F72D729" modified="2022-05-25" version="1">MIKFLFVLVLVFLVAYVLSMEDKERDVYTLRKDKNRCQFPKCGGYYVRKIGNNEKEVYVAKYESLDHYVVDELLQDYLFRGSAVRVLGDNYDIFRIKQIFRALPHGVNSTMQTGYYVVRWGVPTYYGEKGEYYIKDCGEAIDIGSKQSIAIDNYTEPYYQYGNVNNIHYGLLKTNIRSGGIVTGTFDNKILHIHKIFIRVTRSLWCPVIEDSECFKGNINTYMIDEDYCWIATGCQKQPTCPNQKKKVTCKKGYELIQVPTMENNNLGCPVFYCHPEFLVDNILPFNQTVAGSGFEKKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYC1</accession>
    <name>A0A8J4UYC1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Kinase</fullName>
            <ecNumber evidence="8">2.7.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_006974</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>1D-myo-inositol 1,3,4,6-tetrakisphosphate + ATP = 1D-myo-inositol 1,3,4,5,6-pentakisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12717"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57660"/>
            <dbReference type="ChEBI" id="CHEBI:57733"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.140"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>1D-myo-inositol 1,4,5-trisphosphate + 2 ATP = 1D-myo-inositol 1,3,4,5,6-pentakisphosphate + 2 ADP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:32359"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57733"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.151"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the inositol phosphokinase (IPK) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.-.-" evidence="8"/>
    <dbReference type="EMBL" id="AJWJ01000347">
        <property type="protein sequence ID" value="KAF2071713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYC1"/>
    <dbReference type="OrthoDB" id="338650at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051765">
        <property type="term" value="F:inositol tetrakisphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008440">
        <property type="term" value="F:inositol-1,4,5-trisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.160">
        <property type="entry name" value="Inositol polyphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005522">
        <property type="entry name" value="IPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038286">
        <property type="entry name" value="IPK_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400:SF51">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE MULTIKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03770">
        <property type="entry name" value="IPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00036164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00036525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2071713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="282" mass="31674" checksum="E22589E766C27109" modified="2022-05-25" version="1">MSTTEEKSKLKPLATQIAGHSEEDGGSQNLPGFLSSDDGFVYKPVPGKRGQNEVDFYESFHTLDPILQSFVPEYKGLTEVNNTRYMGIANATHGFKIDEISLCDIKIGTKTYGSDASEEKIRLEEAKCAKTTTSSLGIRFCGAKLFDPSTKAIVKLDKDWGKKLKIDNIFDQGIKKFFTHSFDEKISKAIIKEFLVLLNQLLDYFKSINRTRGFYSSSLLFVYGIIDKSIQPTPSSIKIVQDHPQYGISLKMIDFAHVDSIHGKDEGYIFGLENLIEMVNRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYU1</accession>
    <name>A0A8J4UYU1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005107</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000195">
        <property type="protein sequence ID" value="KAF2073590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYU1"/>
    <dbReference type="OrthoDB" id="10665560at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="349"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="280"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="407" mass="46486" checksum="B8865255C595E679" modified="2022-05-25" version="1">MMLASKLFRTTLSSAKNNRLVLSSSNSNRLASTTRYYGTSQEPRLTVRHPEFKNTQEKVDPNIKVPKNEHDDDHHGDHHHEKLALFKPEYEIPKEYDQPNGVYYLEDHHDHDAAHGGAHEAHYEEPSNEIMDDLESRYPIGDAQNYKEISDISEFNASSLIPRVNLTNTAAVDASLLKRAQDAQLLFKDELSVISKESTAAADAADVDIQDPEVDRMLNTLIQDVKEDHDIKQYLDLIQGRQKASVNEFSSSLITEADLENPDEEAIRKRIEESFKSEKATATTAATATAAGAAHKHTDDHHHHKEGEHHDDHHDDHHHHVDPDDVEDTAERGYYQNRPPNSKSPIHPIFVYGVLGIPVVYALYIARTTKKEDKKTFTQFQEDYFKYNPELKERYVELTKTNYPLSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYV5</accession>
    <name>A0A8J4UYV5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="1">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_006587</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="1"/>
    <dbReference type="EMBL" id="AJWJ01000311">
        <property type="protein sequence ID" value="KAF2072088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYV5"/>
    <dbReference type="OrthoDB" id="2020758at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF23">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE USP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="4">
        <location>
            <begin position="152"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="327"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="467"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="675"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="748"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="867"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="957"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="356"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="336"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="467"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="483"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="750"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="869"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="895"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="970"/>
            <end position="984"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2072088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="991" mass="111678" checksum="E26AF13230C65816" modified="2022-05-25" version="1">MGKGKDKKKKLKNQKSLVENNKKKGGQSNKESKEKKDVVKIIVDSQGTTLTLKRIDNLYVCTDCIDQFNLSSKKSNGAVLDKPIPLECSICHMGALEGSSGVSSSTSSLSSSTTSLSSSVTDNKKKRANGNSRFYDDDVEIIGGKAIGDGVKGLDNLGNTCFFNSIMQNLTHVNLLRDLFLEAPPGSPKSVITSNSSMTNEMFNFYSKMYKSNTSRISPHGLFSEIVKKSPRFGGFKQQDSHELLRYLLDGLISEEQNATKKRKEPTYIDKVFGGKLISIITCFHCGYVSKTFEPFLDLSLPISSEKSSNKPPPNFVMPRVAKPLPLKPKSDSLDTATTTTTTTPTVKLDASGNRISKHQLKKLRAKEEKERLEKEEKEKQAAVEKDESIVNLEDDFQVVPIDGKSDENSPVSVNIQVNYSSTGSVTPNGAEEIIDTNNSPKQINIIEIDDDVDNNNNQSQQIKINVKNNNNNNNDNGCSSDIEGEEQESKEDDDNDDQVVISEREGGVELIQATTLLDEISTVTGSIIENYENPDYKPIVLLERSANDLTKSFETLHINGNDQPESDTEKEDDVQKDPIDLRKDIPSDAFNIGSLLSCLLQFTNPEKLEGENGFICSNCMKIHKEKKKQQEEEQEHLNGGSSQVIDIDMDDKEEGQEDEVVEVLVDSEPINNKKKKKKTTPIIKPKPKDEDDIEKFRRNASKQYLLSETPPFLTVQLKRFMLTRIGYQKNSKQVTFPVTLDLSSFTDQNNINNNNNNNDNSNNSNENDNSSSNDNKNDNNNENNTPPTIISKDNNIYRLNGIVEHMGGMGGGHYVAHVYDDQKDQWYYISDSNVRTTTLTQVLNSEAYLVFYKKDNFDVSSLFPSRKSKQEEVKEKEEEIKEEEKVNDQQQQDNSKDDKEQEEKQEEKEDDQEQKEEKEPLLSNNEKTVDIDVEEKIENKPIVIIEKYQQEKEKEIDTIEFKDDDDDSKIDGAELPRTEKKELPIPIEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZ64</accession>
    <name>A0A8J4UZ64_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_006155</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000271">
        <property type="protein sequence ID" value="KAF2072538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZ64"/>
    <dbReference type="OrthoDB" id="273067at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040102">
        <property type="entry name" value="WDR41"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22805:SF2">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22805">
        <property type="entry name" value="WDR41-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25178">
        <property type="entry name" value="Beta-prop_WDR41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="62"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="103"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="376"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2072538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="458" mass="51976" checksum="8B5785B1715F0B9F" modified="2022-05-25" version="1">MFGLFSNKKKKEDQDNQNGGAGGNNTNSAGGGGSNSNIPSSPSGSKEFPSFEISPLNEIQILSSHTQPIKTLLNIDHERLASAGDDNVILVWSISTGRLLMRLEEHTQRVTCLLSFGNILVSGSMDKKIKMWDLSKPAIYNQETKSFHLESSKTLDKHQKSLKFLEKIPNGFCSASNDENLIIWDEQGEFKRYIVRIQREYITSILCVNNFIIVSGSTAPFLIAYKYDSQNETPKTLGKEITHKDCVNCLMNLSARNFITGSKDNSIIIWDSYTLQPLHQRYCKSSISYLYPIKNFILVTIFKGFSIFDMNGNSILEYYPSDGSLTTELNGAITLYDNTKIVTWADTRISMWSWPYELLGGKKTKYPLRPTLIGDLKGHTDYINSLTILDDNSIATGSSDCNIVLWKDGRIQSMNRNSISNRMYNEMNSRYNPLYDFDSIYYEDDRIEYEETMSSFEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZJ7</accession>
    <name>A0A8J4UZJ7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UbiA prenyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_005521</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000222">
        <property type="protein sequence ID" value="KAF2073168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZJ7"/>
    <dbReference type="OrthoDB" id="203513at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042371">
        <property type="term" value="P:vitamin K biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026046">
        <property type="entry name" value="UBIAD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929">
        <property type="entry name" value="1,4-DIHYDROXY-2-NAPHTHOATE OCTAPRENYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929:SF0">
        <property type="entry name" value="UBIA PRENYLTRANSFERASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="17"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2073168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="294" mass="33895" checksum="5C31EF42C9C5AFC4" modified="2022-05-25" version="1">MSINYRKLEGFIHGSRLVRFNLVISYILVGFTIGYKESGQFPFSCLFVIPIFLCLFSVVSILNALCDFEAKIDIKETSVDRTMFDYGLSVKDIWNFVYFNLVLITIFSFLSFWDWDFKLKCYGFLNIIMDSIFCLFYNLPPFRLKTKLFGGEISVLLSYLSIVNLSYCLVTKNPSLTICFIHFPAVVFIAWGMMVNLFVDMEDDKRDGSFSSAIFFGETITKFLIVIIPTLIYISTIKYAIEQNNMFALLPLLTVPLKYIVIELAFKKNKQAKPTFFVSILIFNGLYIIGNLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V048</accession>
    <name>A0A8J4V048_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_009946</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000862">
        <property type="protein sequence ID" value="KAF2068732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V048"/>
    <dbReference type="OrthoDB" id="15252at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052109">
        <property type="entry name" value="SRRM_Domain-Containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34755">
        <property type="entry name" value="SERINE/ARGININE REPETITIVE MATRIX PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34755:SF3">
        <property type="entry name" value="SERINE_ARGININE REPETITIVE MATRIX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035162528" evidence="3">
        <location>
            <begin position="22"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="641"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="551"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="551"/>
            <end position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2068732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="660" mass="73626" checksum="B5B88BB4ED4C4895" modified="2022-05-25" version="1">MKCIILLTILIGILLPSFTLASSSAPSIKIDDDWTVPSWAQISSNSGFFSEGTSAKVNLNLGVFDVAWKQIEPTRDTYTFTSTAVVKEMDFASFNSQNSTKKPFWMRIWNSGTLWAPAWVQTYCNVSSIGKDYDGEDHLPIWNSCVWTEIKKMYTHVFVTLKMREDPRLKFIYVPGAFNWCEFDFTEIGAYAKNNNLSYNTFNTWFQMAMQDLVDIFGPYSYKLVYTGEDYPFDVDMWPKERNLLARDAVAKGMGIRNGITELFNFHLSELPAYGVNIDPNGYLAFNQSWILQSNPNRIIGTENECFNDCGYSVPTKEIYYAVKMSNLKALQMGVNWLYLVETTSYMANYPDHYNWVRYSLGKQASSAFDAWAVLRNAQDTFWADDNSKTWVGKPYIKNLERLLYQRDIKGGAYTRNGSVMKSNIYANVPEQGIAYEGRRTEISTGNTTMSFFVDDGFLFGNLVDIQIKVTYLDLSVGKWIIRYKDNTVTSDTAEVTVGSTQQYYTATFNFTSISFSNSLVSGRNCDFEIVNTGSSDIDILFVRVIKLSSNSQPSTTSTSSNPTSSSSDPTSSSLSSTSSNPASSSTSSSSSSSSPSTSSSGTTSSSTSSTSSSTPTPTSGTTSPSDKENEPSQAYRINAVTSYSLMLMLLLAVQLFIFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V050</accession>
    <name>A0A8J4V050_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003540</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000112">
        <property type="protein sequence ID" value="KAF2075150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V050"/>
    <dbReference type="OrthoDB" id="10461198at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035311977" evidence="1">
        <location>
            <begin position="25"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="95" mass="10550" checksum="143633F3105C550A" modified="2022-05-25" version="1">MGKKSLGLLVGIGLLAFLTNPSTKEKFDAELHKHIDNKKGFFSSLVVKGASLLEFFKIQNCLLFSIAFLNDPNKDQPKLIGFGAFNTWVFDTKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0F8</accession>
    <name>A0A8J4V0F8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009511</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000728">
        <property type="protein sequence ID" value="KAF2069167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0F8"/>
    <dbReference type="OrthoDB" id="270970at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09487">
        <property type="entry name" value="SAM_superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF17">
        <property type="entry name" value="FI21816P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="1">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="597" mass="67026" checksum="7369770F3BD9553B" modified="2022-05-25" version="1">MLGGTSDPYVKLKYNNVSYKTETIKSTTSPIWNFVCKMPMVTNSFLDLEVFDWERFGSNRSIGKCSVDMETIISLSSEGINDKWFQLDKKGFIQIGFTFTPPFPISPNVNRDSITNQQSNNNLDPNFNNNITSFSGILPPPVYLKPIFIQTLLTESWSAQATFSFPGLYSTKNIIAGQSFQASVILTVSAPSIQIRSMVVTLVGKTLNKGKKKQNLIQDYRDLLLSNSTFSGIPLQSKTTFLKGKHIIPFQFYLPKHCLSSVSIHDYSVQYIMSLDIDIVNLPDIQLKQNIVVVNLEDTVNKETTSTIEASTSKSPLTGGNIGISIKSSKSSFYQGEEIELDVQVVNNSQKKVKNIDIKVNRLDYEGTNITPITTNIVTDTKRLYPPIQKGKAHDQLIVVELPTNSNLIHSLSISKVMRVEYSLIVSLDIPKCVDLAIKVPISIVLHDPTHVSPLPDPLSSIASIPRYIKDWSPVHFISYIMFRKNCPEAITNNLDFNFYKLTGPELMTIEKSNLVSILQFAGPRTNELVDSIYDLIYQIVGVRNFLKDLQISNFISAFEKQDITFDLLPSLNHNDLTNLGLPIGDVKRFLNKIKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0J3</accession>
    <name>A0A8J4V0J3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_002913</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000086">
        <property type="protein sequence ID" value="KAF2075780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0J3"/>
    <dbReference type="OrthoDB" id="19923at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 68F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808:SF3">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN GACL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="4">
        <location>
            <begin position="152"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="80"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2075780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="703" mass="78712" checksum="912AC171DA6428A4" modified="2022-05-25" version="1">MDIELFLTAPQGGSSVNSSPSTAKSAVGVASASVPSQTITPATKIKVRLIVEVNGTQYEKSIDGPVDTLKPLVQKLNTKIKKQEESNHKKTHPQLKSGSSLNLFPKNSMASTSSPSLSLNSLTSTFSAREIKDFANSNSSNSSSQDTTTFGVSIDTLVAREKPSNEPKIPTFVKNVINHLFLYGLGIEGLFRVSGSQVEIQTRKSSINKGDYNFIKEDNPHVITVLFKQFLRDLPEPLCTHELYDCFLATSDQINADITRDNGYEVLKKTIQMMPVNNRVLLQHIIYFLTFVSANQSINLMGPVNLSRVFGLNIFWKKEVGQMDIQKLQAISEKVNFITEKMIQNYSSIFEDSGALIAPSPSSSLSNSSKKLFLNAKLLGHKKSIQWMVLTDKQQKVWSLDSYGNCRIWDSQTHQFIKEFQICEGAVYQMISASNNTVWTATGKSVKIWDANGGLIGEIPGESYSLCESQYGEIWVGSAQVLNIYNLEDVPTVANPDELFKPIGTDLFMKGTFILAMCRVSSNRIWGCSSERSLFVWDSKTKETIHQAEIQEKRPKRMTCVETDESETIWIGGDEGTIQIFDSITFKLVHKISNQGWDKIFHIANINKEIWTSTWDTYVRVFDPKSRETLSDYSKTHSDVVSTILEVPNLHGGDSFVWFGSYDKSVTVYTFKENNKWLLSNFKKSAPRPQGIRPVGFSSRLAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0R9</accession>
    <name>A0A8J4V0R9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TLC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000132">
        <property type="protein sequence ID" value="KAF2074728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0R9"/>
    <dbReference type="OrthoDB" id="10672762at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="217"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="298" mass="34091" checksum="8B2EDA920C661731" modified="2022-05-25" version="1">MGSHCIDNSYIDPYLLYPSFTKANGSLGMVSNIEPSSILNRFGLDLEYSDQMLIQNIVLFFLTPMIGYLVLHTFATEIIIEKGPKQLSDKFKLYKQTNPKKISETYQRIVRSIYSVYVCKVSLKACLEGDLQGAIYSLLGAIFYHAGDLITLSLYKSDDTMMWVHHIIAICGCTSALFNGNRRVILMITGCVYSEILVPFSTSISMMRLLDINSGKLPCFLYGISLFIFAILRIPKHSYYLFQSLYYNLGYGVSIWIFFILLPEFKWTWLLIKNFKRSLKTFNCKIIDDDGTLKSKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0S2</accession>
    <name>A0A8J4V0S2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transcription elongation factor SPT5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_009091</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the SPT5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000635">
        <property type="protein sequence ID" value="KAF2069587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0S2"/>
    <dbReference type="OrthoDB" id="28901at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032044">
        <property type="term" value="C:DSIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="6"/>
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    </dbReference>
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    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="225"/>
            <end position="252"/>
        </location>
    </feature>
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            <begin position="1"/>
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        <source>
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            <dbReference type="PIRNR" id="PIRNR036945"/>
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    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2069587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1025" mass="115364" checksum="61EDDB4FBAE95F12" modified="2022-05-25" version="1">MGRSDDDEEYEEEEEEVLDDDNEDYDYEEEEEEERSSKRNKKSSFFDDEVEVANGDDDEDDDEEAEDGWEEMPAEEIARTRDRSIYMDNNNRADADIKDFKNRFVKSYRDHDDDRYDDDEEDYMDDNIPKQSYWRLKCRLGEEKLFIASMMQKMFNNINHPTDKVLIKSIMSPQHLPGHIYVEAEREVHVKTAIKGMSQLLPNAPILTPLKDIIEIISTSKKNVDLKKGTWVRIKLGKYKADIGQIVSYDPSKSRVTVKIIPRIDFAAMTEDKKNKPTNTAANTNEDGTKKKEAPKRKRTRPQARFFNPDEVEKMGLLMTKTATTLHGSFYVLNNEKYKDGFLYKPFRLSSIIVDGVSPSLEELEKFQDREEKVGSDNEDYSRNSRSDASAPLLPKVVPKSTHFSKGDTVKVIEGDLKNLMGIVQTIEDGQVTILPVHDNFKEILYFKPHELQKYFKVGDHVKAIAGRYEGETGLVLRVEDLTIVLLSDLTMSEIKVKPQDLQECTEIATGKLELGNYELHDLVQITPQKVGVIVKVERDSFKILDEGSNVSTVKLQEVGNKRRNKSITTLDAHNNTVTAGDGVEVLDGIYRGKQGTILHISRNSLFIKGRDIYENGGVFVVRTSYCSLLGGSKNKLNNNNNSNGGGYNNNSNGGGRDFNNNRGGGGRGEFSGRGGMGGGGGRGRGRDDGFLHKIVTIKSGPWKGYTGIVRECTETMAQVELQTNSKRLNVQKTNIALPGERPQSQDYSDNNSLYHASRTPMHRSEDPSMTPMRRSNDLWAMRSNESSSSSSSNHWFDDNNSHDYNSTTPGTNYSTYSPYTPNTPSEFSHHHNSGGNNRQDNYSTPYDRNTPMNLNTPSNNFDTPTPGYSSSGYEHSTPQDIPASPFTPHNAPQTPATPSTPANEDQEEEEIEAQFWVGVNIEVIFREGPHQNKHAMVLENTTESTCKVELLDGSKEIVSNVSQNILTLAPPIKKDKVVVVKGKYASYTGTLFVLDKDTNSGIVKVDSNLDFKVFKMTQIAKLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0W5</accession>
    <name>A0A8J4V0W5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003739</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000121">
        <property type="protein sequence ID" value="KAF2074953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="21466at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="22"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="199"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="290" mass="33659" checksum="56E4B86D6789F30F" modified="2022-05-25" version="1">MALDLFTPNKRKKIDSYSQYSNNQLQSQLNFSEENYDTLDEQLFEIENAKEENRVESERLAQIEQILSLREKELNEKEFSLEEKEFEIEEEKENLRKFALELNSTVSDIDIKNKELVEKEKQLLERERIQEKVYNEKKKKLFDFEMSLKKKEHELKLRELDSLLDSNKFAMNNNNGNGNNNINGNGYSTINTVVEDEQQSQQQQQQQLQPNTTTSSIPKKIVARKSLLSNSKLVHSSPAKRLIRRSIIPSASNGLKSNLGSLKENNPNNSSSNLVQTKIRRPSILPNNIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V113</accession>
    <name>A0A8J4V113_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000056">
        <property type="protein sequence ID" value="KAF2076560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V113"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="88" mass="9833" checksum="8511F0239342E960" modified="2022-05-25" version="1">MDKNTNNSLPSTNSQQQSHNNNSSNLYGKVLPDQNAFSPGVSENSLTKTPDPPKRVSSYLERHRPRQNTDDDEDVDGDDVDNTYFSSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V146</accession>
    <name>A0A8J4V146_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000053">
        <property type="protein sequence ID" value="KAF2076680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V146"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="97" mass="11023" checksum="3A5C5B055A326F86" modified="2022-05-25" version="1" fragment="single">NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNINSPSKIKRFVQNFKLNRRNSTIRTPPIQNVQSPLLSPTTSSNVRKRNLSTPNSPNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1S5</accession>
    <name>A0A8J4V1S5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_001279</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000029">
        <property type="protein sequence ID" value="KAF2077430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1S5"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="165"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2077430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="452" mass="51599" checksum="69972CC9312CFC7C" modified="2022-05-25" version="1">MEVLTNQLLSLELNQSTEKMVVLDLNKSSTTITPTSTTTTNLTTTTTTMTNSTHIKIVISPRKPLKSPSKHFTKLIKRDSDSSVFKGSTKLFKITNQSSGGSRCGTQYHHHGDEILDYTCSDTSSSSDSIDDDEVDENNHRQQTINQLLYEEETIIERNPFLDQYSINRLIGEGATSKVYLITNKKTQQRYAAKVIQTVKIFKKDFSVISKEIGIIKHLDHPNIIKMHDCILTSENIYMIMEYVSGIELFDEIVEKRNLLEKDAKKIIYEMLSGLAYLHSKGIAHRDLKPENIKFDSMDSDSQIKILDFGFAKYFNSNPSLNNNQNTNTNNIQVGTLGYEAPEILRNQQQSVAVDMWAVGVICYILLCGYTPFLSYPDYKEPEVLDSYPFWLLFNEDTPYLRNSILNCDFSFPSAQWDAISSEAKDFISKLLVCDPNQRLTCYEALLHPWFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V209</accession>
    <name>A0A8J4V209_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin-related 2 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002695</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000077">
        <property type="protein sequence ID" value="KAF2075988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V209"/>
    <dbReference type="OrthoDB" id="337735at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20558">
        <property type="entry name" value="CYCLIN_ScPCL7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF108">
        <property type="entry name" value="PROTEIN CNPPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="385"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="552"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="78"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="387"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="439"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="552"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="613" mass="69919" checksum="5568DA188B817A4F" modified="2022-05-25" version="1">MYSKSHSSSPKPAAFLTALPSSISAPSLLLLQKQPSLGGSHRKPKFNKNISNSNNSSHINSSLNSSNSDLKSKDKSNKNFIKNNNNNNNSSPVNNNCISNNSNKYKKNSIGNNRNNKNNHNNKSTISSSSIVLGQSRADQSFTDEQQEHMIDVFNKLNINTVSTTTTTTTEKILDKNILQDPESYDTIITKTVSTIKDEEEQEKEVVVKEEICPISIDEEIFKQYTPFLSVLSDMISNMLTDEDLDNNSSLSDQLQVFNVDQVPDISIHDYIQRVFKYLPFGTDIFIFSTIYLDRLIQKNPKIISKLNIHRLFMASILASSKFHNDKSLNNRYYAQVGGVPLWEMNLLEVNFLTLLNWNLFVDTEIFNAFKNSIQSKIDKETEELVTKNSSNNNNNNSNNNNNPLSESTTSSTSTTPTTVSPPQTPKTNPVVKLQIKDNNNNNNSNSNQNNYNNNNNNQNNNNNMKRSTTNNNFSKIINRSRNNYQQNQQNHHQSNYLTYPLPSQPALQQMHHHHGIYNAYYQNYQYFYQQPQPQYYVTSTPFEYQYHSNGSIDGSLQSTPPTASPSLDGGYIPNSHVSSFQQQQPQQPHSWQNETYFYPSTQQQYSSNHFYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V261</accession>
    <name>A0A8J4V261_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pesticidal crystal protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007494</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000403">
        <property type="protein sequence ID" value="KAF2071192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V261"/>
    <dbReference type="OrthoDB" id="23795at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.10">
        <property type="entry name" value="Pesticidal crystal protein, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036716">
        <property type="entry name" value="Pest_crys_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35598">
        <property type="entry name" value="ENDOTOXIN_N DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35598:SF2">
        <property type="entry name" value="PESTICIDAL CRYSTAL PROTEIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56849">
        <property type="entry name" value="delta-Endotoxin (insectocide), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="416" mass="47997" checksum="005349348C876265" modified="2022-05-25" version="1" fragment="single">LFPQEDPYLRKEDFEKRMKKLITDMENKMDKKIDDEIVKFCHSKFESLCSAGSGFAEIEYIYDKNKKAGKPTPDSVLRYMAANHATFRNLLLECLIFFSREDKIDLLCKLYLQTVVMYIAFLRDTHFFGIDWGLPEETVYGDEKITSVAHKAHNTIELAHTYYSKFFKKLNPPRDYRFERQTMIIDENGLSYMGYIPTQFKCDYRPILAEMTYADPTRYNLNQIWLQPTTKEGKVEKIDSGEGIFAPLDLDFGDYEYRLPVSNRIYIADFSKMNCMRGGFGPTEDGKNSVNSVTGTRGMNSALSNGLFFKCFYFTEPKNLTFRVLANIDSNAQIKMVIREIPEGKKAENPDSSREVATKSHSENVAYKKLFESTSGPMAEFVSKQYKFENPGSVYFEAEVVGGDTRNLYTLEIIDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V284</accession>
    <name>A0A8J4V284_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ABC transporter C family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_010126</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000934">
        <property type="protein sequence ID" value="KAF2068549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V284"/>
    <dbReference type="OrthoDB" id="29192at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030587">
        <property type="term" value="P:sorocarp development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18579">
        <property type="entry name" value="ABC_6TM_ABCC_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18580">
        <property type="entry name" value="ABC_6TM_ABCC_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002822">
        <property type="entry name" value="ABC transporter C family member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000010">
        <property type="entry name" value="Multidrug resistance-associated ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044746">
        <property type="entry name" value="ABCC_6TM_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044726">
        <property type="entry name" value="ABCC_6TM_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF172">
        <property type="entry name" value="ABC TRANSPORTER C FAMILY MEMBER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="223"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="800"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="841"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="929"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1030"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1053"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="111"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="500"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="803"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2068549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1202" mass="136546" checksum="71B278BBD93B6D54" modified="2022-05-25" version="1" fragment="single">MSLDNLIRDKFRYLKLDDENDNTNYFDQPCPEDSATILQKASFSWIQRVLWKGYFRGPLELSDISDIPDYMKVNSSTKYLNDVHLKTKFTLIKYMYTHFAARYPLVPIVRIVALIFSILTPLSLKLFLHYIQEEDKSIAAGWGYSFLLFFCAFILNVSQQYGYWSGMKVSLEIRGALISLLFKKMLLLSNTAKRLYNVGRILNLVSADINIFQEYFWNVYVDIIVFPLQIIALLTFLCIIVGPAGLVGFTVMILSIPLTSICASKMSNYFCIALEHSDERVQLTSEFINGIRFLKQYAWEKIFLDRIQEKRTLQLAFLYKRVLAWMLNQVIIQSTSAVVLLSTFTVFILLGHELTPSIAFTALTIFVNLRRPLEVFSQNIQFFNSLMVSSERIKEYLMCTELQDLPFPLFANQYDANKKKSIHKQSQGDIKIVDGNFDWNDGVEHSSSIIVDEVGHSMGNANNIILTNSNSYSNLNEYLINDGADESPIEMNILNQKMKSQEQDTGRDKEQLGAQDPYGTFRNINFTAPKGKLTVICGQVGSGKTSLVSALIGELYKVDGQVTRPNSISYTTQQAFLVNATLRENILFGKPYDKERYANVIYACSLNTDLVQLPGKDLTEIGDKGINLSGGQKQRISLARALYSDAECFILDEPLSAVDPEVGKHLFDHCIQGMMQGKTRILVTHQLQFIPSADHIVVIQDGNLIQGTYSELQQLGIDFESIMKTKKLNVQQQEEEEKQLSKSSSSIKSSREIAPVEAIISDENDADIIEKSKLLVKEDRSAGALGFRVYKDYIKSGGPLWFFFMGVVMSYFISQILFQGSDYWLSVWTSKRIQPDPGNKFYLLVYFGFIVSFMILLGLRFFLLSKYTFSASRHLHSRLLNNVMFASCQFFDTNPTGRILNRFSKDISDIDTIVLEGFSDVLYCGTSCVVAILIMIVVNPFICIPFLFLTMFYYVIQKVYVASSRELKRMESISLSPIFSSFTEAYNGLSTIRAFDQQERFMNDIHTKIDLNVRLYLYSFAVHRWVGTRLEFIAAVSVLLSALFSIYNSGINAGTSGLAVTTALSLTGMLNWSVRQYTELEVRMNSVDRIKNYIDTPREGKRFTLEDDSDQETRLLDHRWPQHGGIEFKNVEIRYRKTADPTLKDISFTIKPNEKVGIVGRTGAGKSTIGVSLFRMVETSKGVISIDGVDISTVGLHDLRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2R6</accession>
    <name>A0A8J4V2R6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TRAUB family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_000031</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AATF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000001">
        <property type="protein sequence ID" value="KAF2078660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2R6"/>
    <dbReference type="OrthoDB" id="5783963at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025160">
        <property type="entry name" value="AATF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039223">
        <property type="entry name" value="AATF/Bfr2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012617">
        <property type="entry name" value="AATF_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15565">
        <property type="entry name" value="AATF PROTEIN APOPTOSIS ANTAGONIZING TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15565:SF0">
        <property type="entry name" value="PROTEIN AATF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13339">
        <property type="entry name" value="AATF-Che1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08164">
        <property type="entry name" value="TRAUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AATF leucine zipper-containing" evidence="4">
        <location>
            <begin position="180"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Apoptosis-antagonizing transcription factor C-terminal" evidence="3">
        <location>
            <begin position="397"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="41"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="82"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="121"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="134"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2078660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="480" mass="55062" checksum="A843BA093D0256BE" modified="2022-05-25" version="1">MVGPAKKVISNTRGGIGKSKAAKTKGDEILDVVTNKSNIKSIDPENVDFGDSYSMKSTDYDNDDDFDNDMLEGKRIKGDMPSTFTTGKYSGQKSSRKSEFIQNDDEDDEEQDQDDDSGDELDSKYDFEDRVGDMDDEDEDEDDDIVGSSNTDKADALLKKLQQQEVDEPILVSKESEKSEMEKSINTKNQSDLYDDFLTTRIQLQKLLVTSNRFPKPKNFNLFIEADQSEGGNSISKKFKEAKRESASLLADLYDLQYSLMNQNDEIPKDDDIKNNNSKRIRDVSSSLNDIWSILEEQNQKLMVFHNSTIDKWNNRIGLTVATGNNKNLKSINQSILSQINNTLSDFERLQKRTKLKRVTYKIFGENDQQQTTQQQQEDIIDQDEYDDEIFDDSDFYQVLLDSIESDEVGSQYWTEMRNLKKKKKKKVNQKASKGRILRFEIFPKLENFMTPQPLPPSNWNIDQLYSNLFGGLGAIKNDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2X8</accession>
    <name>A0A8J4V2X8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GH84 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_009456</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000713">
        <property type="protein sequence ID" value="KAF2069224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2X8"/>
    <dbReference type="OrthoDB" id="16653at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011496">
        <property type="entry name" value="O-GlcNAcase_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07555">
        <property type="entry name" value="NAGidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52009">
        <property type="entry name" value="GH84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035221978" description="GH84 domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="GH84" evidence="3">
        <location>
            <begin position="28"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="355"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="355"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="509"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS52009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2069224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="551" mass="57625" checksum="A6BB8A9B65AB242C" modified="2022-05-25" version="1">MKSINLILLCTLVFCMSTITHAQGTLNMKTGLIEGFYWEQADTVNGQYASYSYSQRLLLINLMASNGIGVYWYVPQTVDLMASWTSSQITEWTQVATLANNKGVRLVFGLRPHYIASNNFAAVKTRLEQIRSVGIKYYSLNFDDAEGDASQQLLQKDMANYLSTNVANMTLSFFTPNAYHMDEYPNRAAWESALVTVDGISSTVPFVFTGEEITPSYMTTAQYPTLSSGRPKVFWSNWIAVDTSANIPWGMIDNNAEPAIFNTGYYLNLNFPLERAIHHIYCLGALSTGSTSCNVNNIATSWATWLNTNGFFHYSYTITQVATSLKNAINNDVYYPTIAQLETAYPQLKGLFSQAPTASPSSTSSTSSSPGIVSSSSGQVSSTGQSTSTGNSTSSSSGQVSSSSSSSGQVSSTGQSTSTGNSTSSSSGQTASTSGQVSSTGQTSSTGNSTSSGITASTGGQTSSSSSTSSSSSSTAGESNPNTAGAGTGTSTGTSTGSGTSTGSGTSTGSGNNSPTSSGGETEEPNSAPKNLNPTAIIGLSLIILIVSMLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5F7</accession>
    <name>A0A8J4V5F7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rab GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_004244</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000146">
        <property type="protein sequence ID" value="KAF2074462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5F7"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001072">
        <property type="entry name" value="Rab family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2074462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="23013" checksum="E93B7C727AC476BC" modified="2022-05-25" version="1">MACCGGSGNNAQPTFNDRKSTSYLHLFKYIMVGDSAIGKSNLLLQFVDKRFTPNSEFTIGVEFGSRSINVNNKQIKLQIWDTAGQEKFRSITRAYYRGAVCAMICYDITRRDTFESLTSWLSDCRKFSCGDVTIVLIGNKTDLDSNRQVSTQEAKEFAEKNNLLFFETSAKTGHNVDLAFEKSAELVLEKLQKNPELNGNNVSVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V870</accession>
    <name>A0A8J4V870_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Plus3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_001140</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000026">
        <property type="protein sequence ID" value="KAF2077522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V870"/>
    <dbReference type="OrthoDB" id="20033at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990269">
        <property type="term" value="F:RNA polymerase II C-terminal domain phosphoserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.200">
        <property type="entry name" value="Plus-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004343">
        <property type="entry name" value="Plus-3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036128">
        <property type="entry name" value="Plus3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13115">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN RTF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13115:SF8">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN RTF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03126">
        <property type="entry name" value="Plus-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00719">
        <property type="entry name" value="Plus3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159042">
        <property type="entry name" value="Plus3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51360">
        <property type="entry name" value="PLUS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Plus3" evidence="6">
        <location>
            <begin position="256"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="75"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="89"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="146"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="159"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="195"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="220"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2077522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="608" mass="69707" checksum="BEEB6A0F0303FB71" modified="2022-05-25" version="1">MAKGRNNMSDDDMDFDDELLSVIEKKPAAKKRKDDSDEEVNTRGGKRGKATTTTTTGRGKTLTKAGGRKGAAAFVEDDEEEPDDGYDQDLYKDEEDRIELSKLPEVEREDILASRYEKRKELRERWEQQKRLKKAKESENRAKGGSQSSSSSAAAADYESSRGSRRGDKEGGKTDQALKDLKQKREKAQSKPPKESSVSSSSTSTKRPPSPSYHYNSSEDQERERQRENEKEREKENREREKERETAKEVQKQIEALDIQTLNQVKLSRNMLVKWVDQPYFETLTPGFFVRVVIGSHLDNPIYRIGEILDIRIGHKLYTVENKETDKILVLSYAGAKKDFSIENVSNNVITPSEHERWITDMVRAGQPLPDPQKIQEKIKDIQKANDYVYTNEDIEKRAKDRARYNKVPVNIAFEKAKLIAIRDTLDPNSEKYQDVCDQIEEYTKLANDLRAKVMSSSEDGIAKINQRNKSFNFRQQYSQTMPETVNNEFDPFARRKTRSIAVVQVTNEDGSKPITAAASSPSAPKVIKNIEVEKSEQKRKPQDYSLQEIHKAIELEIKIPDTVSKILSVQPLQKKRPTIPVHVQGVDHSFKSIITIEDYFKKIHQFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8K1</accession>
    <name>A0A8J4V8K1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000775</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000016">
        <property type="protein sequence ID" value="KAF2077897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8K1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34078:SF2">
        <property type="entry name" value="DUF4190 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34078">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="18148" checksum="772ADD76B5ED3B5A" modified="2022-05-25" version="1">MSNYSPQQGYVDYQQPGQQPQYGYYPQEGVPPPNVVYAAPPGAYAPQYQPQQQYYPPQQPQVIVATIAPVSPIVAAPCYNNDDSHVFGAVLLFVLGFFFYIPWVFSFIYCGSRNKTAKGFAIASIVLFFFFLVALIVVLPIIFVVVVASNNRHNDSYYYNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V9B2</accession>
    <name>A0A8J4V9B2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000072</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000002">
        <property type="protein sequence ID" value="KAF2078572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V9B2"/>
    <dbReference type="OrthoDB" id="24472at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053019">
        <property type="entry name" value="GATA_zinc_finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23353:SF23">
        <property type="entry name" value="PROTEIN HAIRLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23353">
        <property type="entry name" value="RAB-GAP/TBC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="657"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="658"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="725" mass="85476" checksum="E3D41C9E398AE13C" modified="2022-05-25" version="1">MTTTSLFFSIFKNKFLFKTIFDYCKYQDKVGYGYYDIPLRVLFKTNRFELFDHRWSLAKIDQNIFKNKTITEILSSPLIMSQFDFNPLNIDQEDIVEFFNHNTSLERLGRVWPMFYPLIKKPVYNISNIILVGLIKVLSVTELDQYISTIFGPKFLPNMDQISKGIGVYGSVEAFEYSIKVVSFKFGDYKPDIYSYWTQCMIQSCNAQKLNLFYHIADKHLPLLIADRKKINLNSFHSREVSEFLVKSVPLENLTLSNFPYLDMCDFNLLKPLLTNRKYPYINFPLAFKMGDIKLLEFIFQNFTTLLYQVPSYFANLECLKLFFKQKTKYFDSEIESACFHSVEMLRYLASDKGFSFSGPSFNAPYGSLVFLTSNKLFKRLSYDCSMVTSTFHYDLRIVQLFHNYFKDLFYIDTHVLLRFISRGCFGIVKYVLSNNLFKGSLNAIHFYQAATQEMLDCLVAYRRNQKYHEEFFAGDYIDHLKKSNTINLPNIKLIKQISQCKCILDLYPTHFSDLRAISPSFDVPIVYQTLFEGFNVDTFLKSSFSKIVNYAIQNKCPKLVKYLAQHIKAKQIYIYLPLGHQEKTLKDSSPMWDQFQFFNLISPDKKYHCLFLTTLILLYKEDRMEADLLENIFQNLKSTSNFRLLKRLIDLQIPMKNNNNNNNNNNNNNNNNNNNNNTYYKSKKPKTDLDLKQIQYLLTNQIYSHDEIYGNFNDNLSFNDVFEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V9E7</accession>
    <name>A0A8J4V9E7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000048</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000001">
        <property type="protein sequence ID" value="KAF2078677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V9E7"/>
    <dbReference type="OrthoDB" id="2372305at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031083">
        <property type="term" value="C:BLOC-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024857">
        <property type="entry name" value="Cappuccino"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16230:SF3">
        <property type="entry name" value="BIOGENESIS OF LYSOSOMAL ORGANELLES COMPLEX-1, SUBUNIT 4, CAPPUCCINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16230">
        <property type="entry name" value="CAPPUCCINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="191"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="97"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="218"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="250" mass="28605" checksum="C5CB800DB670E39A" modified="2022-05-25" version="1">MEKTIVTSPQDEYLTGIETDDLLRSVAKGYSNYFAFSNSTNEQAKNIDDTMEYMLARIDEFGAFIETIKSDTDKTEKKLIPLLYEKSQQVQQLFPLIDNLTGLMADINKNISILEDRVNQADKHVSNLTGTSNPFKWVLSHLQKEDDVLQQIKNAQALENNSNQPKFTPLKSHNTTEFFKNLRDEMQKVYSNTEKDSSTTNTTTATTTTSESTNNTTSEEKEKEKEKDKQEEEKEEKEEKEKETDNSGAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V9S4</accession>
    <name>A0A8J4V9S4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2-methylcitrate dehydratase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_002281</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PrpD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000061">
        <property type="protein sequence ID" value="KAF2076424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V9S4"/>
    <dbReference type="OrthoDB" id="10055203at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047547">
        <property type="term" value="F:2-methylcitrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019679">
        <property type="term" value="P:propionate metabolic process, methylcitrate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4100.10">
        <property type="entry name" value="2-methylcitrate dehydratase PrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.120">
        <property type="entry name" value="2-methylcitrate dehydratase PrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012705">
        <property type="entry name" value="2Me_IsoCit_deHydtase_PrpD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036148">
        <property type="entry name" value="MmgE/PrpD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042183">
        <property type="entry name" value="MmgE/PrpD_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042188">
        <property type="entry name" value="MmgE/PrpD_sf_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005656">
        <property type="entry name" value="MmgE_PrpD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045337">
        <property type="entry name" value="MmgE_PrpD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045336">
        <property type="entry name" value="MmgE_PrpD_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006943">
        <property type="entry name" value="PRK09425.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02330">
        <property type="entry name" value="prpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16943">
        <property type="entry name" value="2-METHYLCITRATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16943:SF16">
        <property type="entry name" value="2-METHYLCITRATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19305">
        <property type="entry name" value="MmgE_PrpD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03972">
        <property type="entry name" value="MmgE_PrpD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103378">
        <property type="entry name" value="2-methylcitrate dehydratase PrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MmgE/PrpD N-terminal" evidence="3">
        <location>
            <begin position="18"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="MmgE/PrpD C-terminal" evidence="4">
        <location>
            <begin position="284"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2076424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="482" mass="54199" checksum="E61373F4D2522513" modified="2022-05-25" version="1">MTTHTIQVEQQPWDKVIQDIADYVINYNINSTQAYQTAHYTVFDAIGCGYLALKFGECTKLMGPVVPGTAVPNGCRVPGTNFVLDPVSASFNIGCMNRWLDFNDTWLGKEWGHPSDNLATVLSVAQYVVNHQHKSVTMRDVLTALIKCYEIQGVLALENSFNQVGLDHVVLVKVASVAVATHMLGGNRLEIMNAISNAWIDGQALRTYRHHPNTGSRKCWAAGDASSRAVRLALMAVNGEQGYPSALTAKIWGFYDVYFGGKEFRFQRPYGSYVMENVLFKVSFPAEFHAQTSVECAIALHPEVKDRLDDIEKIVIHTHESAIRIIDKRGVLKNPSDRDHCLQYCTAIGLIYGDLHSGHYEDMVALTDKRIDELREKMHIVENPDFSFDYHNPEKRSIANSITIHYKDGTKSDTVQIEYPIGHPKRRSDALPLLEKKFFGAIHDSNLFTSSNSKLDQIKSLYNDMEKLNSTSITEFINLFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VAP9</accession>
    <name>A0A8J4VAP9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carbohydrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001312</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000030">
        <property type="protein sequence ID" value="KAF2077384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VAP9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35885:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35885">
        <property type="entry name" value="CARBOHYDRATE BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035243720" description="Carbohydrate binding domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="337" mass="37135" checksum="F279B5BEC41E7F53" modified="2022-05-25" version="1">MKFKYYIVSILLFELLCTTVTSIHVPVDYKSSSGGDQDSFGIGIWNGDDVSINIGTIDYTNPGSEATLQLTINDFTFPGDAITQSTYNYVTKHLTFVALTPYSVQGYMLTVDCVAWKVVGKIFLPVGYTFSGLASDQTSGKSSIFTTFSNGMGVQIVKINPYTQAMNFLGGPINGAYQTSAYDPVHKQYYLGYSNQTGTYIRTYSPPSKIVQEKQIYFQGQTPPILIAPFHQRFTPLTNLIMGTFQLSNYDSQVINGIGFLDWDLGCFNMTQMLGLDADTFITTVPDSKNDLIYAFTYTVNPDVATLDGGIFIKTFNSTSAQLVSTKPYNTAILAAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VB81</accession>
    <name>A0A8J4VB81_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">VASt domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_000802</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000016">
        <property type="protein sequence ID" value="KAF2077924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VB81"/>
    <dbReference type="OrthoDB" id="20285at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140268">
        <property type="term" value="C:endoplasmic reticulum-plasma membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120015">
        <property type="term" value="F:sterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051482">
        <property type="entry name" value="Cholesterol_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319:SF4">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16016">
        <property type="entry name" value="VASt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="819"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="7">
        <location>
            <begin position="494"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="259"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="345"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="684"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="769"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="28"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="264"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="286"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="297"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="360"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="371"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="424"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="684"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="700"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="710"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="775"/>
            <end position="791"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2077924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="872" mass="96735" checksum="82F8C19D082173A5" modified="2022-05-25" version="1">MASLPNISGQGASLSPNINGHHTIGGVSHNSLTTSPFNGSPPQDIQSPSFFSPVMCSQDNSSSEFYGSTNSTPGLKKKDDKSNQSDLNKLKVRSQSTEELKLFASDEKNKKSKLFISPKLLIPSKKRMDIDEPPPISKTLDGRVKSNPYIKKFKLPTDEILLHDYSAALYKQILLHGRLYLFTNHLCFESKIFGIKTTEIIQIKDIIQIRKKNKNLAVAIEISTQSQKFSFASFVSRDKSYKEIIEVWKEVTGVSHEDASSISVDDDDMDDDTSTTYDDSAPVESNLATDSELQPPNGNHNNGNSNGINSSINRVASSTTINSEPKTLHPGNPSDEVVTEHLLKTSNTSNNHNNIDKKQPSSINTINNSGTTISQQQQQQQQQKQTESITTTSISTSTSTTSSSTSTTSIEETKISSNLNNSSTVTTTTIPDTKPTTATTATVPTTTTTTTPTPTPTIATESKEKNHLDEVYGSIENHLSKEPAQSILESQSTEFQEICTENFNVSVVNFYRALYSDTCNFAYNYHVKRGDKNVVVKNWAFRERYGTIRELEYVAPVNSPIGPDKTRIQETQRYHLTKTKLLVETDTIMLDIPYGDHFRIEAKWDITETSPETCRLTIQLCVRFIKKTWFKGKIESGTIKETKASFSQWILLAKQEIQKLIQLKPVSTTAAQLQLPLNKSVDNKKIEGLKDDERTDSPKPHHTRSRSRQHLINSSSQVNNTLSPGGSHLHINVGSSGGVSPTVVTPTSASIANLQSPYSAPFPTIVEEEKKKPLSTSSSDISTINNNNSSTSSMDKKTNLFKFKSPLGSVSFSGPKGQLPIFIILFFILIIYIFMYIKIVSLSSKLDTMENIFKDTINQQLQLNNNNNNRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJL2</accession>
    <name>A0A8J4PJL2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_010006</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family. DDX46/PRP5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="AJWJ01000883">
        <property type="protein sequence ID" value="KAF2068667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJL2"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17953">
        <property type="entry name" value="DEADc_DDX46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000079">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056149">
        <property type="entry name" value="PRP5/DDX46/KHDC4_KH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23469">
        <property type="entry name" value="KH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="11">
        <location>
            <begin position="443"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="474"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="663"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="871"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="7">
        <location>
            <begin position="443"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="27"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="47"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="85"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="97"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="159"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="179"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="316"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="333"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="345"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="361"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="873"/>
            <end position="902"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2068667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1028" mass="116444" checksum="12F2062DA806D405" modified="2022-05-25" version="1">MDDIEKKRKREYEDDIKYSNSNSGGSRRFDQKPPGTDDRSNSYNGGRNDDRYYNNNSNRDRDRDYRRNDYRDDRYYNNNSNGNRNDYRDERGDDKYYNNNNNRYNSSSSSSNPNSYSSSNSTSFNQSGSSNNGSNAPPPIVFKQATGRGFSSSPPPNVQQQQQNIQKQQQQQQQQTAIDLERIKKEREEEKQRDQEKLDEEMRKRREKVELWRKQKMEAEAKNKPQGNTTATAPNNSSTTLATTPATANNKWSLEEEDDEDDSKQQPIASTSSLPPPPPPMLFKQDFEPMMKIQQQVKEKEKEKEKEKEKEKEQLQEEQDDDIDPLDAFMENLNKQQQQQTKPVNSSKSNQQTKQQKEVLENGEDAEGFEEESDEDNQQDKTVKKGKREILAVDHSKIDYAPFEKNFYVEVPILANMSNTEVQDYRSELGIKINSKKCPKPIQLWAQVGLNEKIHLLLKKYQYEKPTPIQAQTVPAIMSGRDIIGIAKTGSGKTLAFLLPLFRHVLAQPKSAPGEGMIALIMSPTRELALQIYAECKKFSIILGLRVACVYGGASISEQIGDLKRGADIVVCTPGRMIDILCANNKRITNLKRVTFLVLDEADRMFDMGFGPQIMCIIDNIRPDRQTIMFSATFPPKVENVAKKILTRPLEIVAGGRSVVSSEIEQFVEVRPDETRFRRLMELLGTWYHKGQILIFTNRQETTDNLFRQITASGYECLSLHGSKDQMDRDETISDFKNKIKTILIATPLASRGLDIKDLNLVINFDCPDHLEDYVHRVGRTGRAGNKGTAFTFITPDDDRYATSLVKALQQSNAKVPDELLKLEAGYNKKKSEGKEVLYAPTGFTGRGHKFDAAEEEKKELQRKLTKEGLCVPTTTSPTTTGGTTTTTATTTPPPSTTSTGTQDNAPISAVQAINKFLVESIKPAARHHGSTHFVEELEINDYPQQARWKVTHKDGLNTVTDFTGTTITTKGTYFLPNKLPQPGERKLYLYIEGPTETAVKSAKAEILKILDETSAHGTETRKYSHNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PK65</accession>
    <name>A0A8J4PK65_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010464</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001131">
        <property type="protein sequence ID" value="KAF2068210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PK65"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051092">
        <property type="entry name" value="FYVE_RhoGEF_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673">
        <property type="entry name" value="FACIOGENITAL DYSPLASIA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673:SF262">
        <property type="entry name" value="RHOGEF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="2">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="253"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="348"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="364" mass="40747" checksum="8B5D8CAF8815EA71" modified="2022-05-25" version="1">MEKFTIYYQYVKNYNNAYKISSILEEECEDFVNLIQVFGYLPSYLILPIQQTPRYVLLFTDLFRNTNVQNDDYQNLEQLINLDEEENINIQLNLNKNTTNNNTKNIKRPASASSKRKPTNSLAMHKKAIDAVLSENNNLINNNGSNEKKDYSYLPSYQRPTKASASMSRSKVELNPPKPTWRFSYNSSDLFESHYVYNNPTPSIKPPSDTFIDRSTPKNAAVWRPTGSKTVELDSPHSTFKTRNRNHSSIYASSQFSKPSSSSSSSASPSLSLNSSNSSINSNVSSSGGSSNNNNIVGMKPPLFKNLMVEKQRSNPNFTHNNNNNNNNNNNNNNNNNNDNNNSGNGGTSKNSADTAPLSVTLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKC7</accession>
    <name>A0A8J4PKC7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HssA/2C/7E family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010328</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001042">
        <property type="protein sequence ID" value="KAF2068345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKC7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008455">
        <property type="entry name" value="HssA/B-related"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05710">
        <property type="entry name" value="Coiled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="98" mass="8493" checksum="25B892DFD28C429F" modified="2022-05-25" version="1">MTILASLSAMGNPKLGVSSSVSSSSGSGRSGANMSACGGGSSGGGLLGGGGGGLLGGLLGGLLGGGKSSSAPSTPSNGGTYLAGNGGGASNGGCPCCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKQ9</accession>
    <name>A0A8J4PKQ9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Eukaryotic translation initiation factor 5A</fullName>
            <shortName evidence="8">eIF-5A</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_009096</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Translation factor that promotes translation elongation and termination, particularly upon ribosome stalling at specific amino acid sequence contexts. Binds between the exit (E) and peptidyl (P) site of the ribosome and promotes rescue of stalled ribosome: specifically required for efficient translation of polyproline-containing peptides as well as other motifs that stall the ribosome. Acts as ribosome quality control (RQC) cofactor by joining the RQC complex to facilitate peptidyl transfer during CAT tailing step.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="8">eIF-5A seems to be the only eukaryotic protein to have a hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the eIF-5A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000636">
        <property type="protein sequence ID" value="KAF2069582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKQ9"/>
    <dbReference type="OrthoDB" id="9975114at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045901">
        <property type="term" value="P:positive regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045905">
        <property type="term" value="P:positive regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04468">
        <property type="entry name" value="S1_eIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000007">
        <property type="entry name" value="Eukaryotic translation initiation factor 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000034">
        <property type="entry name" value="Eukaryotic translation initiation factor 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001884">
        <property type="entry name" value="IF5A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048670">
        <property type="entry name" value="IF5A-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020189">
        <property type="entry name" value="IF5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019769">
        <property type="entry name" value="Trans_elong_IF5A_hypusine_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00037">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11673">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 5A FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01287">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21485">
        <property type="entry name" value="IF5A-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003025">
        <property type="entry name" value="eIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01376">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00302">
        <property type="entry name" value="IF5A_HYPUSINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="7 8" id="KW-0385">Hypusine</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Translation initiation factor 5A C-terminal" evidence="9">
        <location>
            <begin position="89"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2069582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="161" mass="17355" checksum="02299A4BBAEED487" modified="2022-05-25" version="1" fragment="single">KMSDNEALDVEEYAQAGSGASLTYPVQCSSLRKNGFVVIRGFPCKVVDMSTSKTGKHGHAKVNITAVDIFTGKKYEEICPSTHNIDVPNVSRQDYTVIDSSDGYLSLLSADGNMKEDLKHPEDEIGEQITKLLEEGKEPLVSVVSAMGIEGVVSCKVSNAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKU0</accession>
    <name>A0A8J4PKU0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Carbohydrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_010017</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000886">
        <property type="protein sequence ID" value="KAF2068660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKU0"/>
    <dbReference type="OrthoDB" id="21537at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF5">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035250843" description="Carbohydrate binding domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="4">
        <location>
            <begin position="422"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="3">
        <location>
            <begin position="662"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="792"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="792" mass="87760" checksum="CE06EB9CB63979C8" modified="2022-05-25" version="1" fragment="single">MFNKLLSVLICLLFISTIQSASIVDIIAPEDVYGSSYGCTDRLYQVIVNSATPVNVASVNGVIRVVNSLVNGTISVHDVSITFPNVGRNDQSLTLIDDAGNSQVFPNVITYYCTTVPTYTLATEGGWAQSMATPVYSWYKFISLNGLSKPTEYSSLGCSVSAPFICQVIQYPAILTSYYYIIYLYMTNENVPYSFPANVEISNSYGTNKLIFPFNEIPAGPNTISNIEVYPANNSVVSTAVMSEAHFFFDTSNIKVLPSISAGYPYKYDMNVPVYGTPANAKYFGLIPVQGSSAPTNFYLESIAKTGSTRLSPNHQIQYQTPARSNEVPTDATTSSSDVTELGPGFKLFSLKSKTHTYFGFSYSESGNGSKPFETTYPYGITNGNVKEFYHACTKFVDNYSPSSQAYCAGLSFNNLQNPLATVDDISPSVTNVEVFGLDAEKVLIRVYANDDISGIQKLEFLEGTSNAVTNRDLVKGTKMNGVYEIVAKFISYTYNSPRFTAYDTKTNMLKLKSSKAILNTLTRERVPRYPIYDFIQSTNLQTLEFTFIQFKYNDVDLSTTGINNSLYFNITNAHPSMVPRVRFFATKLDLFDPSINYQESFIEGFWNATIKLYQVDFYLPPRIFTGTVPFNIFMAPNAWESAMLVSAYGAQSQLRVISSNGDLMPPVVSAYAAYPSTAVNVVVNTEIGWTITIQDPINGLKSATFNITSDYDWEPYTIKVTPANVVSGDKYTGVYNIRIPVSVNCRSQIFKISSMVMTDTSDHSSFYPSNGQVNSLYKFINSDQHNININC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLA6</accession>
    <name>A0A8J4PLA6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009615</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000757">
        <property type="protein sequence ID" value="KAF2069068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLA6"/>
    <dbReference type="OrthoDB" id="25620at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF122">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN SKYWALKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="205"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="7"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="90"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="371" mass="42649" checksum="0FCF59D51277DFA6" modified="2022-05-25" version="1">MIANKKLEDFERDNARLQKENWEYKRVGCSNTDHISLFTQLETSNNEKDTLKQERNTINHNVNLINENMEKLLLENKSNNNKIDIFLNKIESLVKVNDSLNNKIQNLSEQNKKSIDNYNNDIGLLFGKIKSLETVNQTLHQQQCTLKEQQQKTLEKIESLTKANESLHQQLIQQINSLESKLDQQNLVMIDTFREMKICKEDLLFLKRSSFFKIINNWIDDSKIIDFQLLYKASQEDFSGSSFHSACDGKGPTITLIETTDGCVFGGYNSQSWNSDGYWYGDDKCFIFTLVNKHGIKPTKYSPEIENICYIGSVKDYGPIFGYGYDIGVGTVNGSRQAFPCTYADTTGKGKSTLTPRKYFIIQTIEIHKCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLC0</accession>
    <name>A0A8J4PLC0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_009707</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000784">
        <property type="protein sequence ID" value="KAF2068975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLC0"/>
    <dbReference type="OrthoDB" id="3512845at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033699">
        <property type="term" value="F:DNA 5'-adenosine monophosphate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990165">
        <property type="term" value="F:single-strand break-containing DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000012">
        <property type="term" value="P:single strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000004">
        <property type="entry name" value="aprataxin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032566">
        <property type="entry name" value="Znf-C2HE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12486:SF4">
        <property type="entry name" value="APRATAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12486">
        <property type="entry name" value="APRATAXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11969">
        <property type="entry name" value="DcpS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16278">
        <property type="entry name" value="zf-C2HE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="4">
        <location>
            <begin position="366"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="148"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="148"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="390" mass="44744" checksum="C6C3353E5ED199A0" modified="2022-05-25" version="1">MKKSLISPTAYKSMIKSINLIKVEDQKIIKIPITEFTVNKHNHDILNYSIGRDTIDSPTNMYISKNQVELILSQEKQTITLISNGVNPSYLKKNNSNNSNKDDIQSILGEEIKDNTFGVDNNDILTFLSPQGKSISFKLEIEKLDLNSNIDNTNTNTNTNTNTNTNTNTNTNTDTDTSKTKQQTTTKTKTIESLEKKLDKTTTPLPSRGGWSDALLPFCQTPQLFPKEVKFYNDNIVIIDDKFPKAKYHFLSMPRKIIPSFNKLSKADLPLLKEMYEQGKLLIDQLPDKDKHVFQFGFHAIPSMRQLHLHLISNDFNSVSLKRKEHWLSFTTEFFIEFNDFYNQLESKGTITIDKEKFESIHKGPMSCPICKQIFSTIPSIKSHYYSHNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLL2</accession>
    <name>A0A8J4PLL2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010283</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001015">
        <property type="protein sequence ID" value="KAF2068391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLL2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="421" mass="48625" checksum="28D921DCA9EEC404" modified="2022-05-25" version="1">MLKDAAASLSSSVNSPSFSVSGSVSGSLDSSNDENEAPSNNESSTTSLILTYGGVPVAFSPAKDGESSPTYSRLFEKKSCDYGSIANMKYPIRLEFEAPDFFLSVDRPEIKITFNDQNVRFKYDTPIKIISWKRNQAILVGSTGVIDNYGKPESAQEYYATTAFESRWAWHTSKHFAKSAFSKGKKTIDCSLITDYCSDLVKFKFDESTKYSDSDTRQVPKDRSFASGQGFFRDFNQPSPIINWFSFKGPIDDKFKKGILQVEHDSRIGTLTRINWVKAYYPNSENTWFFDIKGYHIKEFQEPKYHSKEFIEVNFDKYTPKDPFSPFKWYQLYPFNDAATKESIKTRQLYFYHNYNFNIKGVYSYQLDGNYDANKVPSYDFSFLILFLSFFFKNHFFRCHPLTFRNVVEKDRVQNINTSIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMP7</accession>
    <name>A0A8J4PMP7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008977</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000610">
        <property type="protein sequence ID" value="KAF2069710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMP7"/>
    <dbReference type="OrthoDB" id="4033880at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03571">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000006">
        <property type="entry name" value="Clathrin interactor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF45">
        <property type="entry name" value="CLATHRIN INTERACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="2">
        <location>
            <begin position="15"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="279"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="301"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="374"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="416"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="473"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="504"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="523"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="659" mass="71620" checksum="1FCB23657CFB631C" modified="2022-05-25" version="1">MNIEAVKTYIKKGKDAVLNTPEIERKVKDATSNDKWGPSGTQMQEISRATYHYECFPIIMGVIWKRINDPGKYWRHVYKSLLLIDYLVRNGSPQVIRECRNHTMEIKTLVEFQYIEEEKDVGLSVRERAKQVIELLHDDQRIKEERDKAKGNQNKYVGIGNESRSPYGGGGGGYDSDYGSNNYGGGRDSYGGGRDSYGGGNSYPGSNNRDSYGGGGRDTYGGSNNRDSYGGGRDSYGGSNNRDSYGGNEYSSYDSPSENYNSYSSKKDSAFDDNDFYRNDNTSSNTSSSNRRNNTIDFDEPQQQSRNDNTSSRGRPRAASGGNLQQPSQVVHKEAVPTLIDFSEPSPKPIVFDPLLDANTGSNGGFNNGGFGNFGQPQPQQQQQQQFGQFNAPQQQQQQMSLMGQQSGNNGGFGNFGQPQQQQFGGFNNPSNNSMIAKDPFAKDDFGDFASANNSDFNPFEQPSGDFSNNQQKSKDSNDPWNKKELFDLNNLGKGGLNQSPVHNNNNSSMNSRSTPARAANGPITSAGSTIRPMNQGGMGGMNQMGGMNQGGMNQMGGMNQMGGMNQGGMNQMGGMNQGGMGGMNQMGGMNQMGGMGMNNQGMNQGGMGMNNQGMNQMGGGMNQGGMYNNTGSLGNGMMNRQQPMTGSMGSNNNNAFRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMU6</accession>
    <name>A0A8J4PMU6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008852</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000588">
        <property type="protein sequence ID" value="KAF2069830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMU6"/>
    <dbReference type="OrthoDB" id="20326at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="49"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="137"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="350"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="798"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="474"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="630"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="163"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="249"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="372"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="798"/>
            <end position="808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="844" mass="94348" checksum="F6B86E88BDD2183B" modified="2022-05-25" version="1">MTLSVVPGEPEANSVAVGIQELDSDSDISGDIDAITSLIETSLILEDSEDEEKVSTSSVGDGRGDDKDNRSITTTQSSSILDNIELKHAIKSLTGVFVSDDFISILKKHIALSGDTEIDFEEFREAFSQITRSLYPGGVPNAHSESISNPPSPPPSPTRSRSHSCSSSSAIDLLSPPNSPRNSDSSSPFSFSPVSSSPPKYITYLSTSPSSSHSPPLGNNSNNHHFVSNTTNIFSKPPPISPRRIGNRPRSSSLKTNHVQPSLEQLNDDQNPTLLNSKPISTTAVTVTPSSSIVTSKKPPIVYSSSTGSNGSFSATSSFLNHQIVHQASPSQSHFNQNDLFKSLLALDEPSTKQNKSKSKPISTTNHHHKSNMYNISPNSSSGQLSPQSEDGDHNQNIFMMEELNNMLVQKSTAIKPKEKEKKKKYTYRIDNEEEEISWATEEFYYDPTLTNLDDLHSIISILKEKYSISKSKSNELEKRLQIAIKVNTELEDELDALKKDYIALSLSNQAIRKNNQNLEQSLEFYSSNTNQLRIGCMNLEKENQSLKKKISSFEDTISNNQKLIESTQKEIEYKEIKYQNEIQHLNHQYSFNIQSVKKEYQKEFENEYKKNKECIVSLEKKKSIIELENTKLRELLEISNEKLDNLEKSYQEKTKEYIIQMEEKQNQNVSQEILSTENQKLLNQLNNLKKQWKKIELQKIIDLKNEIKRTKQEHQHYKSLLLQGINDFKSMENTILTNLHPPNHSSLIAGGGGNSNSNSSGIGNITTNTIEATITTTTTTTTTNLNLNLQSLETPTILLSSPHPSHSSRNRIKDIDPATTTSNSDSLVLKKKLSSNKVKKDPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMZ0</accession>
    <name>A0A8J4PMZ0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009481</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000720">
        <property type="protein sequence ID" value="KAF2069199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMZ0"/>
    <dbReference type="OrthoDB" id="659430at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="495" mass="55467" checksum="10290B3F9B649286" modified="2022-05-25" version="1">MKLFPTTIKYLTLNKLQSFYGTCLIPPSVTYLTLCNQLFNDINQYAIPDTVQKIEFKNLVFENQFVRSYDIPSYISNRLVNGVFYIYSKSKNRTTIPSNTTHLFWLDDQYIDNDGDGINMPPSVHTLVLGQTFNSAILSLPATVTQMIFNGSFITPLKFISFPPALKYLSFAYLDKLIQENELPNGITHLSIGDAPNFDGNSVPQSVHHMQYSFGGNCYTQIPPQVKHLKIDIIARGADKIYLPTTIKSFVTSKYDKIIFRDHNLQPMVKRKLPFSSSVDTTIDLICKKNLLIVPYALGNDVKSITFGHGFNQVLLKGAIPNSVENLVFGNSFNHKLNKSILPTSLLKLVLGSDFDQDLNDNLPSSLTELMLNTESKPSFTSSSQFPLNLKKLALPYYDGVLDIIPTTINHLEFNRNFAHVIFPIELVPPHITTLVLNDGIHIQSYDLIPPSITSITLCESITSGTKIPCTVESVVLPSSFNQPLDTILQTVNDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN43</accession>
    <name>A0A8J4PN43_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008403</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000514">
        <property type="protein sequence ID" value="KAF2070284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN43"/>
    <dbReference type="OrthoDB" id="19751at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="249" mass="28402" checksum="0D0586FC22A13B07" modified="2022-05-25" version="1">MKIVNVNEPPRFGLESRKRNKDVNTRKQKQLVKAEKLKKVASEIKKNPNTPINNHTLNILNSNNMNNINKNNNNSNIEKEAPQAGKPNLRNNKPFLIYGEDKDKNALFPSENDIDTEDSVYETSISVRYSSNGFQHELRNVTQEGSDLQKAIQASLNQEDAVILKNPHNEIEDDFVIFSPPKEEEKDLESWELVDECTPSSLVNLISTPYMNETDFPSLIKSQCKSIKKDETNPWNQSKTLLSCITQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNE1</accession>
    <name>A0A8J4PNE1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007554</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000410">
        <property type="protein sequence ID" value="KAF2071125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNE1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="387"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="434"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="389"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="403"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="434"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="447"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="587" mass="69661" checksum="16D88E878A6F0AB3" modified="2022-05-25" version="1">MIGNQNIRLLKRIITTTPTKQWLPKRSFCISTKWSEGGGGNGGTATQIVENAIPLDSLSREFKADNEKVRAQDLSALRAQDIPDMISPLLKTTLKLIDIPIEEQREIIMDFMKRHGYQWDDFYTLKYTEFSKEININRVFKLYSGSTQMMLQAVFPEREWEYWKFNKNSVKFEITREMQLQCIRAYGVENNLDTPDKWYRAGLTYIGKIQTSSSPLSTNEIIQLVVEAHPEMKFSKWRFLRAKLDIFDDPAVKQDFIEYFQSVTKKIDHLEDYYLFKCRDFKVNKFLSHMIKQDFNTKYLKFLEFFFPQVEWSEDIYKVIKTSKTNHLSASKKVFTYTIINQHFSYNPEFLLKHNYISNEQYKEIKAKKSDNSITTSRHHLDQIHQDLNNNSIGNSRNNSYNDIDEFDNNYDIDDYDVDESQDRLVAQEDSHYDEYDDDYHDHDDLGQDEEQEYDEEDEYDYQDLENYDYEYSLQKRFGKKKPNPIHKSLGDVEENDTLLVKGTSSVRVLPYKNSKRSKNRIIRESAIIHVPVIDDYLLTNDTPTPRLKRKLEYKKIKLIKVSDPSRIVDAKRIPRQYKGRTSIFNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNP6</accession>
    <name>A0A8J4PNP6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008174</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000482">
        <property type="protein sequence ID" value="KAF2070508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNP6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="37"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="123" mass="13446" checksum="FE1EEFBEEC5994BC" modified="2022-05-25" version="1">MLKSIRVLSPTLNATASPRGTSIFSSTISTSTRYYSATTTNTNNTNNINNNDTFKQEQKEQQQQQDNSNNGFSQGEQQPPKKKSSFKKTLLLLGCTAAVGSYVYFKANGTEPKYSSSSSSIVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNW5</accession>
    <name>A0A8J4PNW5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008728</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000567">
        <property type="protein sequence ID" value="KAF2069951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNW5"/>
    <dbReference type="OrthoDB" id="6229588at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="52"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="89"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="125"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="155"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="161"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2069951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="189" mass="21742" checksum="680A86ACBBE75524" modified="2022-05-25" version="1">MSSAVAVEHPVADVKKDKKDKKNKKDKMLKLFKKGNEVTLADLTAFLKKQGFDQTYIDTFVKTVDTNNDGKITSQEYQSNVDKIPKEDLSTAELRKQFTKLDKDKSGFLEVAEVQKFLKGNKGQDFEAFMKAKDLNANGKIEFEEFIKFAHENKKVRKEKKVKEGKEGKEGKKDKKEGKEEKKDKKEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNX6</accession>
    <name>A0A8J4PNX6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007718</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000427">
        <property type="protein sequence ID" value="KAF2070968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNX6"/>
    <dbReference type="OrthoDB" id="19292at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="50"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="106" mass="12276" checksum="A8C193F4D80C94D3" modified="2022-05-25" version="1">MNDQEFFNLLNQYPLRDENAQTSHQQQQSQPQSQPNIFSMLNSFMGGMRSPSTPASPSTTTTTTTTTTDNDIFWIKFREFLEKNISDKEKVNQVYNNFKLSQQKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP07</accession>
    <name>A0A8J4PP07_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphatidylserine decarboxylase</fullName>
            <ecNumber evidence="3">4.1.1.65</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_008173</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Phospholipid metabolism; phosphatidylethanolamine biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.65" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000482">
        <property type="protein sequence ID" value="KAF2070507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP07"/>
    <dbReference type="OrthoDB" id="4330at2759"/>
    <dbReference type="UniPathway" id="UPA00558"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004609">
        <property type="term" value="F:phosphatidylserine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003817">
        <property type="entry name" value="PS_Dcarbxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033177">
        <property type="entry name" value="PSD-B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00163">
        <property type="entry name" value="PS_decarb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10067">
        <property type="entry name" value="PHOSPHATIDYLSERINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10067:SF6">
        <property type="entry name" value="PHOSPHATIDYLSERINE DECARBOXYLASE PROENZYME, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02666">
        <property type="entry name" value="PS_Dcarbxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="10" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2070507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="391" mass="44789" checksum="19808A70AED4F3ED" modified="2022-05-25" version="1">MMIVPSPYYYPSTFCTVYDHIDDFDNENTSAKNNKITTTTTTASTTASTLVNHEKEYYLCPNCDVDDYDDCVCFLINHDDQQGKQQQQQQQGILTTTTTNPYYFKVEYLKRIPFRMTSNLWGKIANIKIPENFRKPIYMTYSKVFGVNVNEAERPVEEYESMADFFSRRLKEDARLIDLKHDLVSPVDGTVIYYGKVDNNSLEQVKGLTYSLEEFLGEKEMAKIKGKNLYHLGIYLSPGDYHGIHSPIDWSIQERYHFPGYLFPVAKVAVDNIPGLFALNERVVLSGQWKHGFFSLTPVGASNVGSIVMDFDKELSTNNPKDQSNIRKEKSEYYHKSYTSPISSLRGNEVAFFKMGSTVILVFEVPQDKQFDFTIKPGQHLKLGEPVGDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP19</accession>
    <name>A0A8J4PP19_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_007634</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NPL4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000418">
        <property type="protein sequence ID" value="KAF2071058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP19"/>
    <dbReference type="OrthoDB" id="10251089at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08061">
        <property type="entry name" value="MPN_NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016563">
        <property type="entry name" value="Npl4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007717">
        <property type="entry name" value="NPL4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12710">
        <property type="entry name" value="NUCLEAR PROTEIN LOCALIZATION 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12710:SF0">
        <property type="entry name" value="NUCLEAR PROTEIN LOCALIZATION PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05021">
        <property type="entry name" value="NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MPN" evidence="3">
        <location>
            <begin position="287"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="72"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="201"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2071058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="565" mass="62793" checksum="EF13FCAA915D6553" modified="2022-05-25" version="1">MIIAIRSESGTNKVDIPDNSIIKDVFVKLKGLNINTDGKQLAKTPRDTPLQMGDTLSRSGIKSGDLIYLVGGSSSNNSSSSSNNTSNNNNNKKQQEEEEQNSHSRLTASCKHSENDKCVFCSDAQGVKKCIHPENATCPNCQDKKFTGKKLKWLCNHPPGGKCSNCIRVPKKVEFKCNHGPTAVCGNCIDKKSKQEESESSDSNNNNNSNNSTFSPKVAKQEIKAKCLHGPNAKCTNCLPKDDPNEITPPKRRCKNHGPNGSCIDCIEWRESLKMKLKSQDNPHCPGALVDFQCANVFQQYLQAKKFEQQRCGFLYGNFLEDGTVVVDAIYEPPQKSTQDNVELLNDPIGEKIESLTSMLGLTRVGWIFSHPSRKYIMSSTEIIQTAQFQNKYGTSFVTLILSINNEGQSNLEAFQVSDQALKLEKTAQFSATQTNPLKIKLKEPVFEEGTETINADINFFIVTVPVKAKEGKSKFVNTFPVENRDPSPTLSDLASYKIDNQDQSPLVFFSDFHFLVFLLENHLFDLNTDFPHICEGIKSGKSDRLSGFFELVEAQVGELIGHDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPK6</accession>
    <name>A0A8J4PPK6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_009012</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000618">
        <property type="protein sequence ID" value="KAF2069664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPK6"/>
    <dbReference type="OrthoDB" id="10261634at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="266"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="293"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="7">
        <location>
            <begin position="25"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="330"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="340"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="359"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2069664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="437" mass="48218" checksum="AD9230312736B3F8" modified="2022-05-25" version="1">MGGGGLYREGMGAVSTGSAVKDSLRAGFWIFTWVSLNLALQFTNKYLDVSGFIFPILVILTGTFTTFIGSAVAIFVFKLCEFPYEALMRHKVLLILVALFQALTYVLENISIVTIPLSLNQIIKATGPAFLIALSFCMYKETYTKEVIITTAVIIFGVILTVLNNPGFELEGAAYSLGSVIFAVLQTILIARLLEDPKLNTLSIVSITSFPSTIAIIPMFFTLEYHKLMDYTGPTTVPIVLVVFLAIAACLYNISHFYIVRYTSALYYTIVGNAKVIAVVVASSLIFHSKYSTLNYIGIVVTLGGFISYNVVKYYENIKLAKESKRINDGESIDSSSSVNNNNNNNNNNNDKYMIISTNDDDGDDQDEDVDHHHKIFNTSQDELIHRHVGSGSGTNNGAGNNYLNRDEEIGLVINDDDDDNDDGDENVNLVYKNYDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ43</accession>
    <name>A0A8J4PQ43_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ER membrane protein complex subunit 7 beta-sandwich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_007874</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000444">
        <property type="protein sequence ID" value="KAF2070806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ43"/>
    <dbReference type="OrthoDB" id="16218at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019008">
        <property type="entry name" value="Beta_sandwich_EMC7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039163">
        <property type="entry name" value="EMC7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13605">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13605:SF4">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09430">
        <property type="entry name" value="EMC7_beta-sandw"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035153679" description="ER membrane protein complex subunit 7 beta-sandwich domain-containing protein" evidence="7">
        <location>
            <begin position="21"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="ER membrane protein complex subunit 7 beta-sandwich" evidence="8">
        <location>
            <begin position="54"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF09430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2070806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="210" mass="23646" checksum="03EC896F57D95AB5" modified="2022-05-25" version="1">MKDTGLYCLVLVLCICIVNGQSTTATASKSTAASKVNKLEGKLAGVNDIKYHLTKVNLIDNTNYNEYSVIPDKAGSFAFYNISSGFYTMEIECFQYIFYQYKVDVLNNKIKNQIKVRPAENETIIMPQPLVLKPMSRGLYFQQHVPFNIFSLFQNPMVISIGFTSVMIYLLPKMSSFVNEAAQEEGMVKPKQGPELLQKVPDWGQALITN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQB1</accession>
    <name>A0A8J4PQB1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein mS41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_006629</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS41 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000314">
        <property type="protein sequence ID" value="KAF2072063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQB1"/>
    <dbReference type="OrthoDB" id="18595at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039603">
        <property type="entry name" value="Ribosomal_mS41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019083">
        <property type="entry name" value="SAM_Ribosomal_mS41"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28235">
        <property type="entry name" value="PROTEIN FYV4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28235:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09597">
        <property type="entry name" value="SAM_Ribosomal_mS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01238">
        <property type="entry name" value="IGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small ribosomal subunit protein mS41 SAM" evidence="6">
        <location>
            <begin position="158"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="66"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="66"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2072063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="232" mass="27232" checksum="792540C669B79262" modified="2022-05-25" version="1">MIKNIVSIGIKLPRFNSRVLVCNSSLVLNKNNKQQQQQYIYSSRGFASTSEVNKIFQITNEITKELEDKSPKDNELEKQDEEKEEVVNEFGEEDGSEQDLTIYEFDPLPTDEPILPTFNANLTIEDLIIEYKDPHAPKTVDNYVPYFRFTEPRDGYTVESFLQKIGRNCDSHVGLFERWDDLMNTSPKKLKKAQVPVRERKWILHWVEQWKQGRNPEYISLTRSIAKRNTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQK4</accession>
    <name>A0A8J4PQK4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007597</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000415">
        <property type="protein sequence ID" value="KAF2071076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQK4"/>
    <dbReference type="OrthoDB" id="20736at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052591">
        <property type="entry name" value="CML21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23064">
        <property type="entry name" value="TROPONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="7"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="78"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="104"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="142" mass="16736" checksum="DB4019E1CD201F5A" modified="2022-05-25" version="1">MSQFEQQVEKDIQNFLKLYDSNKDGEITKAEMVKYFTDKQNRNPNRLTERIFAIYDKDKNGIITTEEIRGNSIKMKTEMVDTSGDGFISFEELVEDFKKQKADEPELLAGFFFESLDRNNDKKISRGELRRYFEASHTKPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQP5</accession>
    <name>A0A8J4PQP5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_007612</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MSOX/MTOX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000416">
        <property type="protein sequence ID" value="KAF2071075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQP5"/>
    <dbReference type="OrthoDB" id="424974at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008115">
        <property type="term" value="F:sarcosine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045170">
        <property type="entry name" value="MTOX"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008425">
        <property type="entry name" value="PRK11259.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10961:SF7">
        <property type="entry name" value="FAD DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10961">
        <property type="entry name" value="PEROXISOMAL SARCOSINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="6">
        <location>
            <begin position="35"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2071075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="426" mass="48239" checksum="7E4F62D23486A2B7" modified="2022-05-25" version="1">MSTQQPAYLRRIQTISPHLQAPPQTVTKNIIYSTIVIGGGVIGASCAYQLSKDGGNVLLLDQYHMAHDNGSSHGDGRIIRFSYPEDIYIELAKIVYPMWKDIEKESNTKLYQMTGGIDFGPGHLEPIVDLIQNYKKHNIPYELLNKEEAEKKFPQFKFSPTDVIVYQAEGGVLYASKAVKSLWLLCKQFGATVEQNKKVDRIKVESKDLITVLCDDQTVYKGRKVVFAAGGWTNELLYRSQLNLTLRIEVSQENVFYWEPRPDTPKSIDYSQYSNPVAIYYRDADNAFYSLPEIDIKGVKCGYHHSGTALDKMGMPGDRKPYPKEKLDQIKEYIGKYNPGLNNEKESSSLHCHYTNTPDYHFIVDRHPKHSNIIITSACSGHGFKFAPAIGKLVSCMARDVPTPFDISDFSLNRFNNKSLIKRTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR84</accession>
    <name>A0A8J4PR84_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acylamino-acid-releasing enzyme</fullName>
            <ecNumber evidence="5">3.4.19.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_006361</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of an N-acetyl or N-formyl amino acid from the N-terminus of a polypeptide.</text>
            <dbReference type="EC" id="3.4.19.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S9C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.1" evidence="5"/>
    <dbReference type="EMBL" id="AJWJ01000290">
        <property type="protein sequence ID" value="KAF2072327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR84"/>
    <dbReference type="OrthoDB" id="416344at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008242">
        <property type="term" value="F:omega peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000344">
        <property type="entry name" value="Acylamino-acid-releasing enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045550">
        <property type="entry name" value="AARE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776:SF4">
        <property type="entry name" value="ACYLAMINO-ACID-RELEASING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776">
        <property type="entry name" value="SERINE PEPTIDASE S9 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19283">
        <property type="entry name" value="APEH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acylamino-acid-releasing enzyme N-terminal" evidence="10">
        <location>
            <begin position="14"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="9">
        <location>
            <begin position="520"/>
            <end position="739"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF19283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2072327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="744" mass="83704" checksum="2B08B48154FB535A" modified="2022-05-25" version="1">MDTPSPPSSTSENLEYRNKSLKLYKDIISVPTVVNSFFNSSESISIVLSQTDSQTKKNKQFITTKYVLPTADKQVTSTHFLNELVSPLISQSPSHTKQITIKDTTTNPENIEYSFEISDRDHHLTTIPSRDIHKKVCSDEWFGTISWSPCEKYIAFIADSKINTTSFYDREPKDKIVGDQFLYRDDWGETYSPISNPSIFIVDIEKEAIFPLEPFPSEKLTAGQVIWTPDGKGLVFVGWSVSVRKLGIRACFNRISSLYHFDFASYLVTRQEAKDKKEKPTKPLVLTNLIPSVSQGCFRSPRFSPDGKSLIFMGFDEKTFPHNTCSKLFKLNWAGSGVATAGQKPLTLIDYKNHNDEFPGLYCQDIPINPWIDNSTIVFSAPIKSTNQVLSFNIDTKQLNTLFASKCSYSVQDVDLKSKQFIMTESGLNQPTLVYLIDYHHLDKKVLLYKPVVSKDLTDIFASYDFSIHSVPVNIPTPAPFASIKEFELIYLKNTTQKKSPCLLFLHGGPHVSVGTDYAFSLTFLAALGYNLIIPNYRGSTSFGKDFVECLPGLIGTLDADDSLQALLYTLSTLDKDGIDVDKVGVIGGSHGGFLSAHMSKYPQFKAAVLRNPVIDIPSMSTLSDIPDWCFYECGLKPSVDRGDDTLYHTLPTLEEIEQMKQCSPSRYIDEIKTPSLLSLGDSDLRVPPSQGLLYYRSLMERNVPTKCVMYPKTGHSLDTVDAKLDQWIHNACWFNKYILENNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR96</accession>
    <name>A0A8J4PR96_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006508</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000303">
        <property type="protein sequence ID" value="KAF2072188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR96"/>
    <dbReference type="OrthoDB" id="24198at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32556:SF18">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32556">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2072188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="175" mass="20010" checksum="1AC1E2322F07E8D5" modified="2022-05-25" version="1">MDTIDGQEINEDDQEIYCINRSCGSLSRKRIPKYSQSLWKECLQLLKTNTTLTELSIGHYECRCYDGTKKLNQQLIDDLLDSLSNNKSIKTLYLDFQGLNGLKKRHPIINVDFISSLLQNNTTIESLYIGSEDKGEWEKPLFNDIVNSKPSSSKCFIGYAPKMPMSSQFSSKAYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRL0</accession>
    <name>A0A8J4PRL0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_010398</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001085">
        <property type="protein sequence ID" value="KAF2068276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRL0"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002287">
        <property type="entry name" value="Dual specificity protein kinase splB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF298">
        <property type="entry name" value="MIXED LINEAGE KINASE DOMAIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="640"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="304"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="391"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="554"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="914"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="108"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="122"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="133"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="310"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="322"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="391"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="557"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="930"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="668"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2068276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="941" mass="103855" checksum="D4483D071FD4D5D4" modified="2022-05-25" version="1">MGSVSSKTLPNNGTGSPAKSPTISNSNNNSNSNTSSGGSSNDLLKSTTTKNKRNNKRNLSVDSGANNNNHNANTNGSLRESPCSSPPNDEYSLSQRSDENFLEDKENNNNIGNIYESNGSNRNNNIGHLRNTSNNSNASNNDSIKNKSIDIKSCDDLQSSSSFNSKSNSNLSIYSSYSHQQQQQHIQHNDHLIVCENCNHTKTIQLPIQPLTIDDDDNNTVNITTTTPTLTTATFTPIETTFIPPFDDNNNNNTVEKDFNSLTICNENNNNNNNNNNNNNIFNNFNNLNNLLFNNNNTATTLNGSGGNKFLSNSSPTVQTWNNSNNNNSNNQQQANLLASSMGSTSELFKNSLISSFLNNQNLLNNNNNPFGNKSNSNNNNNVINNSSSTISTNISSSSSSIQSNNVNLSSTTTTNNNHLKDISYDARDSNFSGYNYQNQANDLNSIISSVESTYRKLNESSSSNTIVKSNNSVPTTSTSTTTNTIINHTLHSSSNNNSLASNESFNSTASTSFQHTSIRTSTNKANNNHHHHLDDYSKDITFLVSDHTDSIKEKSTVSNASSVTSASNMPRMHPALAPSVFARPRGRSISDSLFMSPFSKDSINDIQKAIENEKIKKTRFEELKSILGDREAIIDINDIQFDQKVGEGAFSEVWEGWWKGIHVAIKKLKIVGDESQFRERFIREVHNLKKGNHQNIVMFIGACYKPACIVTEFMSGGSLYHMLHNPNNPKVKYSFPLVLKMSTDIALGFAHLHSMNIVHRDLTSQNILLDEFGNVKISDFGLSREKPTDGTMAMTNGGICNPRWRPPEITKNVAQYDEKVDVYCFSLVVWEILTGEIPYSDLDGSQASAQVAYCGLRPTLPEACDPELKLLLQQCWESEPSDRPDFSNIVARLKEISWNNPIGFISEQFSQYEGSTPRSAHDLHSNKGSSSSSSHSRSPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRW5</accession>
    <name>A0A8J4PRW5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">valine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Valyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_005385</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Val) + L-valine + ATP = L-valyl-tRNA(Val) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000213">
        <property type="protein sequence ID" value="KAF2073308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRW5"/>
    <dbReference type="OrthoDB" id="629407at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07962">
        <property type="entry name" value="Anticodon_Ia_Val"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00817">
        <property type="entry name" value="ValRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000009">
        <property type="entry name" value="Valine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000020">
        <property type="entry name" value="Valine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.740.10:FF:000005">
        <property type="entry name" value="Valine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000197">
        <property type="entry name" value="Valyl tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02004">
        <property type="entry name" value="Val_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033705">
        <property type="entry name" value="Anticodon_Ia_Val"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004349">
        <property type="entry name" value="PRK05729.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00422">
        <property type="entry name" value="valS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946:SF109">
        <property type="entry name" value="VALINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="VALYL-TRNA SYNTHETASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00986">
        <property type="entry name" value="TRNASYNTHVAL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="12">
        <location>
            <begin position="107"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="13">
        <location>
            <begin position="785"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="21"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2073308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1065" mass="122090" checksum="8F43E4D7C364633E" modified="2022-05-25" version="1">MDQQKEKQPTTQTPPKEIDEETKRKNEEKKKAKEAEKEAKKAKMAEKEAKQAAAKEKELANKAKKEARKKRKRKLKKKLKLKLKKRFSMNVSELLPAYQPKAVELCWYDYWLENGFFSPEKQMEVQAHVQKDKKFVIVIPPPNVTGSLHLGHALTNSIQDAIVRYRRMKGEVCLWVPGSDHAGIATQVVVEKRIWKEEGKTRHELGREKFIEKVWEWKQDYGSKIQGQLKKMASSLDWNREVFTLDETRTVAVNEAFIRMFNDGLIERTTRLVNWSCSLKTAISDIEVDYKDLEKHTKLPVPGHDGLYDFGVLFEFAYPVEGTNEELVVATTRIETMLADTAVAIHPDDERYKHLIGKFIQHPLVDRKIPIITDAILVDKEFGTGVVKVTPAHDHNDYETGLRHKLEMINLFTDEGLINENGGEFKGMKRFDARNAIIEALKKKNLYKGMKDNKMRLGICSRSKDIIEPYIKPQWYVKCDGMAAKAVQAVRDGDLQIIPASHNATWFRWLESIKDWCVSRQLWWGHRIPAYHVNVRGVTPNPYDTTQWVVGHNKEDALANALEKFKCTKEDIVSMEHDPDVLDTWFSSGLFPFSVMGWPEQTKDFTDYYPTSLLETGSDILFFWVARMVMMGQQLTGKLPFNQVFLHAMVRDAHGRKMSKSLGNVIDPNDVINGITLDGLVQKLYEGNLDAKEIEKATAGIKQDFPTGISECGTDAMRFALCAYTSQGRDINLDIQRVVGYRHFCNKIWNATRFAVMKLDIPNYKPPTFDADALLKDTNAINISILHSAQTAITIVEEGFKNYDFSAVTTAIYNFWLTELCDFYLEMTKSIFSMEEDSPIKQKTKDTLYTCIDIGLRLLHPFMPFITEDLYQSLPRRPEDSAIPSIMLAPYPVSQPQWNNTTIKEDIQQCQDVVRAIRSLRTNYNIVASKKIHTYIHVKSQEALDRFKNLSQVIRVLAYASEVDVCISDEHRAGCVSDVVKELSDVSILLDVRGSVDFTAEISRLESKKSTAISNKETLLKKINIPAYDKVPLKIRQDNDQKLKAFDDEINTYTKTIESFANMKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRZ7</accession>
    <name>A0A8J4PRZ7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Armadillo repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_006028</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the importin alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000259">
        <property type="protein sequence ID" value="KAF2072668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRZ7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2072668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="425" mass="47559" checksum="D716FC0DA463F472" modified="2022-05-25" version="1">MDSQQTFDLKAKFSVFTIDDEKQIKETELHLNQIANNIIESNSLEALLSDVVKIRQLSTTGADKIESIVNCNSKLFPKLIDLLNNSDDNPKFQFEITWIFLNVCAGTFKQTKYVVDLDVLGSFKRILAHSKDQQLLIQVLWCIRNISDDDALNQLVFVNGLFDSLLLLLGNIDKTSTELGKQIATTFCNMIINGKHKFSIYNSALPWLNRFINSNDPEIVNVSCKAMNNILCAENDSAIEKTLSFLHKHNPGPRLIEIVLTSSDETTIESAMVNIGDILSSSQEHVMYLLQCNVKVGGGGVVKGPAGLLNAIEWCFSSSNPLIRKAATWALSNLAVEYPQLLIDHHMLDNVIHNLLVDQDMDVRVEAIWVINNVLESSADVSVLKSKMPQITHTLSNELSKKEIKDPLAINAINFIIDYQSNEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS15</accession>
    <name>A0A8J4PS15_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pre-mRNA processing factor 4 (PRP4)-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_005261</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000205">
        <property type="protein sequence ID" value="KAF2073413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS15"/>
    <dbReference type="OrthoDB" id="540662at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030621">
        <property type="term" value="F:U4 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000411">
        <property type="entry name" value="U4/U6 small nuclear ribonucleoprotein Prp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.110">
        <property type="entry name" value="Pre-mRNA processing factor 4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014906">
        <property type="entry name" value="PRP4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036285">
        <property type="entry name" value="PRP4-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19846:SF0">
        <property type="entry name" value="PRE-MRNA PROCESSING FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19846">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08799">
        <property type="entry name" value="PRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00500">
        <property type="entry name" value="SFM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158230">
        <property type="entry name" value="PRP4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Pre-mRNA processing factor 4 (PRP4)-like" evidence="5">
        <location>
            <begin position="77"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="313"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="363"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="405"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="447"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="532"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="200"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="200"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2073413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="566" mass="64461" checksum="218F53E5BC881D18" modified="2022-05-25" version="1">MNSNKIFYGQVKREDVNNNNSQKQQQHNDEQEDVEQQPQHEEFLPFSKHAKRSQSKFQEKLDDHENKKKSKLVIVPTNDNLVKLKLRELGEPIILFGEKYEDRRSRLRTLMIERNIYDGTPLAEKKRLEELEKQAYVHLNQEAFLTEGTEQLKQARIKISLYSLARANERLDSMKKIREKEVEINQININIKEEEKKQLLLKQQQQQQQQHQDESTDGTGNGSNGNGTASTDIKMIGEGGYNIQDKLVKTDFEKEYDGCVDKLKNYYPYFSQIADERPIAMSVFSPSAKYIASASWSGISKLWNENGEHMVTYTGHSQRVSSVAFHPQSGVTASMSSVNMATSSADSTIKLWNLESNLPIGSLDGHLDVVNRVAFHPSGQYLLSTSTDKSWRLWDLETNTTLLDQEGHAESVMGLAIQCDGSLVATGGLDSLVRIWDLRSGRPIHYFKGHNKQVISIDWSPNGYQLASASEDNTVMVWDMRKKENIFQILAHSSIVSCVKYSKTNVGFLTSCSFDAKIKTWSPIDWKPISTLEGHSSKVTSIDISTDNRIISSSFDKTWKLWAHDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS48</accession>
    <name>A0A8J4PS48_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000272">
        <property type="protein sequence ID" value="KAF2072522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS48"/>
    <dbReference type="OrthoDB" id="20803at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="109"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="177" mass="19908" checksum="0F29418590B97446" modified="2022-05-25" version="1">MRIHIPIRRRTNTLSSPTNRLNNSNNSINNSTTSSGEEEEEEAVSQYTENNNDLMTVEEFILGLNKEEEESGEGHTQNVENENCLMTVEEFISSCKGPNQQRGGNLVQSESASNTITNSTTTTTNSEEEEEDLLTVEEFIFTSEKRDNSNDLLTIGEFMAQSSPPGRLYNRSKLLIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTG3</accession>
    <name>A0A8J4PTG3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005489</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000220">
        <property type="protein sequence ID" value="KAF2073207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTG3"/>
    <dbReference type="OrthoDB" id="21263at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="431"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="235"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="448"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="482"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="520" mass="60395" checksum="246B5764BB16B75E" modified="2022-05-25" version="1">MMTSTINNTIVHSNGNGSKSKLYDKSRLESLFIYQNFDRVILDITTILFESYHKQYSTIESFLNNLDISSINSDKTVALVSQCDHQCQSNSNNSCDCIWMMQLLIQSLYEKGLYTLALKFINSFYRDGISNSPFDVLILSIHLLVHYKNYEESKAIILEVITKRYIMVQKNNNQSHQNNILNNQQYEQLVELLIFHIMFRLGESEQCFQYLESDHYLSSWKKEGFIQALNEMIHIKEIEEKSHKELQQKQQKQLERELQDQMNQLQQNNQQQQLENQQKLIETQKLLINQQIPNNGNSNNKKSNLIVEIKQHLFEIVHEILTIRDFESLKTVLTKTVYGNIRKLLHAIITKLKSIIIYLKSNQSKQFIKKAIPGIISTALLFYAVYLLANQRKSLAPAVKKQKLLQQQQQQQLLSPTSSNVANNTVSNVNSTLQQQSQQPRRSFGLKNVSQQQQPQPLRSYSAGSNNPIPPSRYTPQYQQYSSSNRPSMSNNNNTNNTNSYWSSFKQLVSNSFTLNNRVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTW1</accession>
    <name>A0A8J4PTW1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006224</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000277">
        <property type="protein sequence ID" value="KAF2072469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTW1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="578" mass="63590" checksum="5322EE46DF8A7943" modified="2022-05-25" version="1">MTDTLNLESLSIDNESKEINVSLSYLNANHERLALLKNVTITYIAQSKGDYQDFVNSPHHHLVNSLIINSEHIDFDQVKFPATVTKLFLQQIVKLNFNSLGSSITTLLLNIDDTTFSYSNSIPESVTELLVSVPEYKDVPAVSTLLSTGLPSQLTILKLENIALKSDVPVPVTVKDIVLTDCSNPEKLVVKTVEPMPSTVAKIKTLEQLTWAKEQRWLTKLNIQATLELTPGLIGSHVTMVGMFNYKGAVAKGIFPEGLVDLSLGTQSLIEADSLPKSLKIFKLYQYNHVFVAGNLPSGIEDLDLGNYNLPLPVGVLPESLKKLALFRYEHQINVDVLPKGLEYLFMCNSNVAILPNTLPSSLKTLILPSYNKPLEKSTLPASLTDLQLFAFNQELVPGTVVGTEDILPANLERLVLQSFDKPLPIKLNKLVFLDIGALTQDIASVLSNVSKVQINFQSIRDNVELPSSITDLTMERVGSQSVEGVVLQKGFFPASVKTLSLKLYGIQFGADLIPNTCVSLETDQINFTTGSLPQSLNVFRIKHRGISGPNDFVKQYSNTTGIIPTKTVSYNGTLFTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTX2</accession>
    <name>A0A8J4PTX2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_005344</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000211">
        <property type="protein sequence ID" value="KAF2073326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTX2"/>
    <dbReference type="OrthoDB" id="19919at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16148:SF14">
        <property type="entry name" value="MYND-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16148">
        <property type="entry name" value="NF-KAPPA-B-REPRESSING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="504"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="106"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="532"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="52"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="115"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="141"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2073326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="594" mass="67727" checksum="1F9FFDB5FD46A1E7" modified="2022-05-25" version="1">MNSNSHEEIDIEGCPCCYDKEHCSDCCIEQQQQQQHDDQDNHCIECCNKEQQQQQQQQQPEVQEEIKQVEKELVEQKEEIVEKISSSTVTFDENIINNTINAVVQQQQQQHQQQEDYSTPPYSTPPIQCSTPPPHIPIVTPPAQQQQQQQQQQQQQQQQQQQQQQQKPRQQHNIQKPTVNHIIKDKNTLYSNQDSVFDIVLQWGSLAVSKLVFYPIEFPIHALTTFIQSNPAPQPISDTSVTTDIIMNVFGFKPTASRLKTLLSSFTPLISTPQPHPVSTRFIPISQSTSLLSLLKQKGTHIYDGGLVYLLHNMITIFINFIQRSFNNIQNSPKNKNPLHKNARVAIDFSLTLLKYGITLPLDVLMTRLIVHRFYRSDNRFYMSDCTFSGICKNEGIVGLYAGWEVVVLKYLLEFFYTKLDNIIQNKRKSNQTNNKNNNSNNNTNGNDKSPLQIILIGVKILLDYMLTVVKVRLNIRNIGLLKTLKNILTREGMNGFLSGLNTYFMLFPLWMITILVANFIFLKVKSVLEKSEIPPPSPTNNSHIDGDGNIATFDNDHKDSGLGVAMNDLNSLSQDNDNDNDNDAETQSSSSPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUF8</accession>
    <name>A0A8J4PUF8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Translation elongation factor EF1B beta/delta subunit guanine nucleotide exchange domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_004541</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the EF-1-beta/EF-1-delta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000163">
        <property type="protein sequence ID" value="KAF2074138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUF8"/>
    <dbReference type="OrthoDB" id="331763at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005853">
        <property type="term" value="C:eukaryotic translation elongation factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00292">
        <property type="entry name" value="EF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.60:FF:000001">
        <property type="entry name" value="Elongation factor 1-beta 1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036219">
        <property type="entry name" value="eEF-1beta-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049720">
        <property type="entry name" value="EF1B_bsu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014038">
        <property type="entry name" value="EF1B_bsu/dsu_GNE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribsomal_bS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001326">
        <property type="entry name" value="Transl_elong_EF1B_B/D_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11595">
        <property type="entry name" value="EF-HAND AND COILED-COIL DOMAIN-CONTAINING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11595:SF21">
        <property type="entry name" value="ELONGATION FACTOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00736">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00888">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54984">
        <property type="entry name" value="eEF-1beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00824">
        <property type="entry name" value="EF1BD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00825">
        <property type="entry name" value="EF1BD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation elongation factor EF1B beta/delta subunit guanine nucleotide exchange" evidence="5">
        <location>
            <begin position="139"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2074138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="225" mass="24622" checksum="69E5355EF8679B0B" modified="2022-05-25" version="1">MNTSNRFTMPSFTDLKTEAGLAELNTYLADKTYIVGFVPSSADVEALALVGTCPCNTKFVNVCRWFRTISDYTAEEKAAFPKTEAVTVKAAAAPAAAKDEDDVDLFGSDEDDEEYEKQLAERAAAAKAKKAAKPAVIAKSSILLDVKPWDDTTPMDELEKSVRSIEMEGLIWGASKLIAVGYGIRKLNINVVVEDDKVSMDDLEEQIVAFEDYVQSVDVVAFNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUZ5</accession>
    <name>A0A8J4PUZ5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000189">
        <property type="protein sequence ID" value="KAF2073685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUZ5"/>
    <dbReference type="OrthoDB" id="284858at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="58"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="289" mass="32415" checksum="3B76138AF1FD9C91" modified="2022-05-25" version="1">MSAIKISTSEELEKKWDEFSTSFARYYEPNTLPICFLLISNLGLTTNFDGRPTPKAILEVACGGGAGTEMCLNLKKPETSFDACDISSEMVALTKRRLGMKQEENTIVDKKFTISQCDAERLPYKDNSFDGYFANFCLHLVAKPENMLAEAYRVLEKDGVGAWSVWGRPENSNQFTILRTVAANMGLELKPSPVRSAFHLHDTAKLRQMALDAGFSEATAGYTYCNLMIRNGDEFVQSFFSTPEMKITEKEIGSEKYKEWKVKTAEYVDGLLKSGTFIGLESAYLVVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PV92</accession>
    <name>A0A8J4PV92_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004476</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000158">
        <property type="protein sequence ID" value="KAF2074230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PV92"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="111" mass="13221" checksum="79B63D3024F9A958" modified="2022-05-25" version="1">MIKPTYQPDQIRNFGDEEFNIAVQNFHDYSVNSYKHKHYQGFVESMAETAVLCLEKMNKPTSKFTIPKDYQQLRAAVRPVYEEYMALHSIPHVFAMTQILLEREQRQQQQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVR6</accession>
    <name>A0A8J4PVR6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DUF647 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_004211</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RUS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000145">
        <property type="protein sequence ID" value="KAF2074466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVR6"/>
    <dbReference type="OrthoDB" id="19606at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006968">
        <property type="entry name" value="RUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055412">
        <property type="entry name" value="UVB_sens_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054549">
        <property type="entry name" value="UVB_sens_RUS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12770:SF31">
        <property type="entry name" value="RUS FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12770">
        <property type="entry name" value="RUS1 FAMILY PROTEIN C16ORF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24160">
        <property type="entry name" value="UVB_sens_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04884">
        <property type="entry name" value="UVB_sens_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein root UVB sensitive/RUS" evidence="6">
        <location>
            <begin position="106"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Root UVB sensitive protein C-terminal" evidence="7">
        <location>
            <begin position="348"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2074466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="500" mass="56051" checksum="85ADB5948A65C08F" modified="2022-05-25" version="1">MFISKIPKYRYNIHRLVNTTAPQFQRCLSISILNNKYYSTSSTNNDNNSNNVVSIKEIKNGIIHHRYHLHTKDNMLVKDMNHSDGQQIQNQEEQEQHDSIIKKSIYYFQRLFLPSGYPRTVSSDYINYQKWLFFQNTLGSTTYVLSTHALLSSVWGMSVATSLPFAAAISWVLKDGLGASALVLFASKYSTLFDYDIKKYKFRGDLLHNTGVLMEMCTPFVPGYFLPMASVSNLCKGLAGLVYGSTRASLNRSFSLKENIGDITAKYQSQSMAAYLSGMAIGSSVGMVLSLYPMSTVSSLGMVFGLSLVHVLCGYKSLKSIKLKTLNEQRTCLLMDHFIQNGCTIDSVLSVDIVNDQEKFIQSSDYQAFSSRVKLGASVGESLLTCADLASVATQKHKYLIKQSTTDNTLYVVFLDDGRKEEPVSSTSPISISNYTILKSYFHCFVLNNNNNASMPRNDIDQFVDTHFPIFMQGLSKQGWAHEPNQVLMFAVSDIKLAIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW87</accession>
    <name>A0A8J4PW87_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004403</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000155">
        <property type="protein sequence ID" value="KAF2074270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW87"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38085">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38085:SF1">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035310531" evidence="1">
        <location>
            <begin position="21"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="364" mass="40096" checksum="3775482F8A802094" modified="2022-05-25" version="1">MKQISTIILLLTLSISTCYSSYLPTTIPGDVTRTNTTTTDTYSRSFNVTLKSTIGQIVGNTAAGETFDQLNFYFNASNLNVYYDYAIYKSSSPSRYYIGGLYNFNYRPSCIVEYLETNGKPGYQYLQDQVVGWLRLDVGYKFSVLRQSVGSVGVFNYTISPNSQYPANVFSMNMYLSSSAIDVNSVPMASAQSMLSMTISNYYSLTNNRKSSSICDTPADCVSTGPSGYSQSRLAIVSVIYSSTNDFGTDLGKAKMMLTTSEKNLLIGGTWSKQASTQYNTIYSGDTVNVYTSANNTVNGNMFVFEKFGSLSQLLIHSFDTVRPSYLAWNGPIVGVVQDSHDNGSFKVVIPTFLIIVLILCNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWD2</accession>
    <name>A0A8J4PWD2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003719</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000120">
        <property type="protein sequence ID" value="KAF2074980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWD2"/>
    <dbReference type="OrthoDB" id="24203at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF178">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="559" mass="64527" checksum="400EDF48EB934349" modified="2022-05-25" version="1">MNQSIDNSLFFKIIRDKYIRFNIRNYLFNFKIEISLKDLFNNQTLNHLISFNIPLLLKVNQNEFLEYHKSFIKFINNKNNYINNLIQHIVFIDGFNEKIPIGFIGKQVKFVDLGDDFDQDLEIGTFPQDSELEKLVFSYSFNRPIKDDLLPSGLKVCYFSRDFNQCLGENTIPKSVTKLRLLEEMVEQKITNNMLPPNLTFLKFSGTVDRDFKFPSTLTSLSLVSFTSRITTNLFPSTLDTLRFLDVVWSSPLSKEILPSNLKHLYFENDYPYSLDILPDSLTSLDLGFHLNTEFQQPLPRNLKRLIFSLAYDKPLPYPLPSSLTYLEMQNFKTCTFKPGFIPASVKTLVYCQSPSAPLTPGVIPEGVTSLTFPYSFNSLLQHGSIPSSVKHIEFKSIYSFSIEKDVIPSSVESFIIRIKDQELDISLPPENTCSISFYFKANVPLYKPLLFGITHLDYISNKEINSEYIPSSVTSITFRDYNIELKKGDIPNGVKELSLGTSFRKPIDPNTLPDSLTKLSLNKSIYFKFNENIPKSVKYLSLYVYHDHLIKIERMKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWQ3</accession>
    <name>A0A8J4PWQ3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000434">
        <property type="protein sequence ID" value="KAF2070901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWQ3"/>
    <dbReference type="OrthoDB" id="15743at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029027">
        <property type="entry name" value="Single_a-helix_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81518">
        <property type="entry name" value="Subunit XI (6.4 kDa protein) of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="71" mass="8063" checksum="3E800D3F8970B2D7" modified="2022-05-25" version="1">MNRAIQQTNLFIKRSLSFAANWSTTATLWGVTAGVGALYAIQPRFIFKHLPLIGHNYLTAKDIEQMKKNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWR7</accession>
    <name>A0A8J4PWR7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_004410</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000155">
        <property type="protein sequence ID" value="KAF2074277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWR7"/>
    <dbReference type="OrthoDB" id="19923at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07307">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 68F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808:SF16">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN GACR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="4">
        <location>
            <begin position="320"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="264"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="521"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="655"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="264"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="291"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="583"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="600"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="613"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="629"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="680"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="698"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="749"/>
            <end position="769"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2074277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="769" mass="85583" checksum="A14BCECAA749EB95" modified="2022-05-25" version="1">MKKINRAYGEAKVSLSAMINKDVSPTEIPEYIAKVNSIQGIKYFSKDILNGLSKQSKSNQVLIEENAQLGETLIEYGSWYGNSEGGACDSLGNLIKVFGDLHVQMEVCRSKLDIAVANRLHNQMNEFMKNDIKDAFLAKNRYDKSRITFDAASETFKTLRKKPNVNQDKLSDAEQELDYATQQFSDVSTESLYRMEDIVEKYNEEMFDSVYDIIKQYKEYFDKGTEIINQILPEIDQHKKSMAKYKQSMNERKSKRANFVVFEQSNNSNNSSNNIQISSPPPIPTSKYSINSNNNNNNNNNNDNARGSRSSMVALKVFGVPLQVVVDRENRDTPMIVDKAIEYLEKNIKTEGLFRISPNQKTLLEVKQLANTGSIQSFDINYQYDDAHLVSSFLKAYLREMPVPLFTFELYKKITDCVLDDQKDKESMAKSLSEILKQLPYCNLILVKKLFALLYSISVESKINKMNTSNLAVVLAPNILYPKQLDIQAISNSNAAVEFMISQYILIFKPLEGIPAVERKSSELKKSNDTAVLPPRLPTRPASVMFKQSPLNNDKGVLKSQSSSSIAMSPPPLPPKSNRLSVSTSTLMSPPASSTPPPSSLSSSSSTITNPTTPSPPPLSPVSSPPSTPVINNSNSNNKPINNRVSVYLSNFNQQHQSNSPSTATPTPPPKPSRYSVMIPTTKNNINNNDLPPPLAPLPSSSNSSSTASPAPSPPPTFKKRPQPLKVEQGKSVVKDGVKISHSLLDDSVSYSPVTDNYDYTSSDSFYDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXE4</accession>
    <name>A0A8J4PXE4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_002906</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000086">
        <property type="protein sequence ID" value="KAF2075773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXE4"/>
    <dbReference type="OrthoDB" id="18990at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040458">
        <property type="entry name" value="Vid27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013863">
        <property type="entry name" value="VID27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040979">
        <property type="entry name" value="Vid27_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040768">
        <property type="entry name" value="Vid27_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31913">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31913:SF0">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08553">
        <property type="entry name" value="VID27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17748">
        <property type="entry name" value="VID27_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17747">
        <property type="entry name" value="VID27_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vid27 N-terminal" evidence="4">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Vid27 PH-like" evidence="3">
        <location>
            <begin position="253"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar import/degradation Vid27 C-terminal" evidence="2">
        <location>
            <begin position="611"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="421"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="424"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="475"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="492"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="529"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="576"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2075773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="961" mass="110437" checksum="F3603E50CA4D8961" modified="2022-05-25" version="1">MSLFKRLTNLFNSNQPSNLVECTGYFYHYHKNQWICLFNDCELVIHRAGGEFKFNFQIQVTRIVDEELLRQQEEQDGEEHEDEDEGDGDKGVDNYFEFPITTGLELYKETGPLGHRAYSWKGTNNRFMFEFDESESNDINKQNRLARVICECIYESKYHQPASTGSDNEIDNFCRETQTVVMSSLQTSVTTMRPDPIPMQTSSDRVNNSSAQEQQQQQQQQLYNLEELKGTSVPAVPVQPPIQVKGEQLYHQSVALYLETNDQFNLKDASVDVLITRTKSSNPIEYQYYILNQSGVPILSQLIDSNMYPQFFNEQLSFIWYDFSTNEEGLRWSIKFPASKEYENFRQQFTIYNYESANQTKFKGKKDEVEFMAQQFTEDVDDPNIVFDTDDGFNAKDLEDFDKLTLEDDELNDSKEFNKFYDQTQDEDEEEEEESGSGEEELESGEEEEEESGEEEEEEQGESGEEESEDEESQQEITPVKPVKKQPVVQSESEEEEEESEEEEEEEEEESEEEITPVKPPIKRQPVIEESESDEEEEESEQEESEEEEEEEEESEEEIKTPIKTPIKRKPIIESSSEEEYSEESDEDSSDLEEIQDPSLKQISKDFKGAKNSSLVVGYKDRSYVVRGSTIGVFNTGENDIKFNTAITNIKSKDKKNFSPKKIMLQQQDQSLLMLNPEKTSNIYKIDLNRPDIVEEWDLQYKGQPTAVKSIMHQQKNDETTVKPTFVGYSGNSMFLVDPRQKTKNVSVKFTGGSNPRSFYTCAATSDEGHLAIGTDKGEIKMFSKTQFDPTKRYTGSDDPLGPVARSRTTLPGIGDPVVGIDVTKDSKWIVATCKQYLMIIPAELKDGSNGFEDRLGARKPVPKRLVLKPIDIKRMGGVVNFTPAKFNTVLDNSNNSETFIVTSSGRFLITWNFRKCKQNVLDVYQIKEYKSDIVAEQFKCNRDNSIVVTFPDDVVISNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXF8</accession>
    <name>A0A8J4PXF8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">THO complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004866</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000180">
        <property type="protein sequence ID" value="KAF2073839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXF8"/>
    <dbReference type="OrthoDB" id="10257415at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021861">
        <property type="entry name" value="THO_THOC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13265:SF0">
        <property type="entry name" value="HPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13265">
        <property type="entry name" value="THO COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11957">
        <property type="entry name" value="efThoc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="523"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="630"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="523"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="638"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="674" mass="77231" checksum="CBF06BE17E13186E" modified="2022-05-25" version="1">MQSNLINLLKDVVKNNVSLLLLQSKEQNQQESITTDVEITDENNDNLSAPSTPPTSTTTKTTTAPTLSSSPQILEKPIIEQKINAIFPTFDSTKPNETNQVEIRSSFEINFRIFFNDLIKLESTTTEEIDISLQLSFYCTELNYLDSIVPFQLFEDLFDSKSISKCLTLFSLLESRADLISQDSSFSDKGRKKNLLLKICIELLKRFSRSSNPDSCGRILIFLAYVFPLSDPSGLNTKGEHNIHPEETLDLTDMMQYDQETSNGETTTTTETTTIDQNFYKQFWGLQKVFQNPQAVLFTTTTTSTTPTATTTLTLNKVKWDTFIESLEVVINTFSTHINLDELTTLPKKHYFTKYLTSSNLMKLQLKDSIFRRNILTQVLISFQNIEILLQKYPTTLSDAQKHCINDLTIKIYKILSQTQPNGAYFSNCLLSILKREKNWILWKRDHACKSFEKTSCEPIVVKKRKLKKPAKNRVNMGNAVLSRLWNLSSTMSNNNEYLKTQNKVALDTLLDPLKTEMVQEREAAQKEKEKLERRAIKEKARSEAKKKRREQYEIDKAENPSLEFDEDLEMQDIDSDDLDDLDDPKPLLRNDSVYKWKTIRLISRENFSLFKHKHFDELVESSCFPPVAVTPPSIEDNNNNTNNPQEIESTTATTEKESLSSTSPTTPTTAMVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXG2</accession>
    <name>A0A8J4PXG2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Prespore-specific protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007398</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000392">
        <property type="protein sequence ID" value="KAF2071291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXG2"/>
    <dbReference type="OrthoDB" id="21521at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="533"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="585"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="692"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="735"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="924"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1007"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="243"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="263"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="742"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="924"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="958"/>
            <end position="976"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1071" mass="121159" checksum="09B24D885F69F391" modified="2022-05-25" version="1">MSSHKEHSKVLGGTINYRNSTSIIPTSFVSTTTTTTNGSANSSKSSMSATFVSDTTSESGIDQQQKDNNTRPPVPTSQSLSHIESVSPSKKINHQHNNSSGIISSLFHHKDKTELEKLKEQEKARVKDEKKKQKEKEKEQEKQREEEKIRQKEKAKEEKLKTKQLEKEKEKESKERVKELERMLELEKQKQKELKKNRMSVEIDNNNDKDSHSGTVSKRSSLSIFGSGDKAKLQHANSVEQFPTTTISPSLPTSRSYSDKSNKPSSTKSESSAPNTTVTHAATTPIIISGSLDIPNQHLFPLGQHRGQSKLNLHSTKILSGFPDNDISDESDVSISSNNQSMIEHPSVLSDNPPTDFSSASSTARYLQKMQQNKSPNFHSSPPSPNIPASPTLYGADIESSPSLTCIHNTSPPLSSTTPPPHIFSSSPNLTSAPFISETTLKKSAIYKRKLEDFYSYIYECEKSNQRYNPLQDKRRIFQRKTTVQNLLEQSFKSKLNQNINNINNNSSNIIASNNINISNNNNNNIKGKRKYLKPETHRRSHSASPSTSPSDLELEKTISSVSPPPGVVSHFHNLSMGNLESTLLNTKDKDNNNNNNNNSSAITDKAKRKGLLKRTFNPNRLSLTLHHSGSRKHDYLDYNNNSTSNIDNNNNSNTKQPFNILPSFDGGRLKKIQTSVKGLFNDGIAMPTIPIPSLFDKKKKDNNNSNIKKLNKNGKTELTKNIDSVLEDILDDDYDKDDNSSDYSNSSHNNYDSPTSKATRKHSLLETYITRYNLQKKKLRVYDLNQMAARLESKIQLHSLELQEFKSRQQSFSNQLKSTTDQLVESASLSRDFIYTNQERQYKLQSRLDKAKPFLDSTVKSINTFYDKSKTLENTVQMLRRSRKQSNNTFSQFLYKLFMITWILSAFTFIVNRFSHLNNNLNNSNNSNSVGNNSISNSSDDDDDEDKFYDIKDDENNSNNNNNSSSNNNSSSNNNSLIQQQEISIQKRQTKGLNLDNHDSIFKYQQDSLQQQKQKQQQQQQQQQQSLDPIKNNSSNNSNNKNNLKLAKTNLEAMSKTIAMMREQEGEEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYL0</accession>
    <name>A0A8J4PYL0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002362</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sel-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000064">
        <property type="protein sequence ID" value="KAF2076356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYL0"/>
    <dbReference type="OrthoDB" id="442451at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050767">
        <property type="entry name" value="Sel1_AlgK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11102:SF162">
        <property type="entry name" value="HCP-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11102">
        <property type="entry name" value="SEL-1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="76"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="76"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="545" mass="62303" checksum="813326E54DBE234F" modified="2022-05-25" version="1">MLLGRRLTSLATRGALQHISHPSHTIASRCSFRLFNKAQYSTLNTKQYQQTLLYSDSNNSDSNIHSLIINNNNSDILKNNSNSSQTNQENDNDNDNDNNELPSKYLFNRILETLDEKSLSPDEIKKLNQYKEDAQMNESLVDFHIGTFYSQGIVLEKDQDKAIFYYKKAADKDLAEAQYAYGLTLIKGTSENLFTPIFPSLIDNSSTLSTTKSLLDDMILGKGWVSPSNNETDKDFDYYKWLRQERRKFKVYRDNKTKIEKSKRDNLKTGIRWLTLSAIQNYTHAQFSLGLLLLKGNYEVDVNETQGIYWLEKADQEGDVNATYELANYYYNAMANDIENSNLYLEKAIPYYLKGSNAGHAECSYWLGSNFIDNQDSSGINGLEFLLLAIEQQSKPAPTFLSILYRNGVIVEQDKQTFIKYLEMGVDRQDSTALLMMGELYFSGDEGFELNYKKAFQFFKSSSLLGNSNASLNLGVMHFNGFGTPIDYLQAFYCYQNAYHYDSTNISAISNLYTMHRDGLGVPKNVEMAEYYLGLLKKLENINNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYP2</accession>
    <name>A0A8J4PYP2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002846</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000083">
        <property type="protein sequence ID" value="KAF2075860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYP2"/>
    <dbReference type="OrthoDB" id="10617111at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00821">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="137"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="457"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="278"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="457"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="474"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="492"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="675" mass="77636" checksum="6C09D634A2C738BF" modified="2022-05-25" version="1">MTMTTTTTTTTTTTTGSSTATAAFVKDCTYLSSSFVQFCVYVNIKDKLKSFVLHLDFNIRVLLICRRGKKLISIPFSSIDIPRLSLENKKLFTFKTFEPKSYDFYSSSETMALEMVQLFTRISKDPDNYQSPEANILLYQGYIHKKGKVGWSQRWFKILHNKILCYKKRISQYPVNETSIRIQDQTGLRLRNNQRTYLFKTSNQQERDLIYNKIVSFGTERQTPFPSERKKSTQLQINRYFSPFSTLDPIDFKTLHQLFKKPNIDLKAIHLFNSSIESGNNSSDSSSNNTPISQKSTLSSSLSKNLFPSKSNTLTKSVNNNKELWDREEEEEQNNNDSYEENQPIYFRKSSTNRKSKRMISRHSVQELIKKYNSINLSSGNLLANGGVGTNSIYSKLIQRVDNLNQSCDEILEYIQENRDRIPKPLLINLMTIFNDSTFIETFANSGSYDSYDHHSVGGGGGGGGSSDIRLQEESNCQNQNQQCQSQQQDGDSNEEEEQEEEYNTNGNNEIDLKSSLSSLSLSESSLSSDEDNSNYYSDYDKCKKENYYKYEQQLIQQQMQNQVANIYQMKKQQEEPTIYESKNVITICENVPIEYCVDENNNKNSNKMMKQSQFEQEQSCQTKGFTLSPKHSIGLKMGEVNNTFPNSRDSSLMKPLSLDLRRYSRDLIPSKIST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYQ4</accession>
    <name>A0A8J4PYQ4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Clu domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003288</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000101">
        <property type="protein sequence ID" value="KAF2075409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="19516at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001329">
        <property type="entry name" value="Small GTP-binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025697">
        <property type="entry name" value="CLU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027523">
        <property type="entry name" value="CLU_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT EIF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601:SF27">
        <property type="entry name" value="RAB GTPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13236">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51823">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Clu" evidence="3">
        <location>
            <begin position="857"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="206"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="469"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="592"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="805"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1323"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="207"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="221"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="330"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="491"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="592"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="605"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="657"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="814"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1323"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1375"/>
            <end position="1407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1660" mass="183558" checksum="30D2AE99D03D9FFF" modified="2022-05-25" version="1">MTSEKPLKILIIGDNGVGKTSILLRFADNTFNVDPKVTLFIDSRIKILEVYGKPVKLQIWDTFGEEKVGQLTSSFYKGVSGIIIAYDSSSRESFEKVISKWSIEARRYAQPTTPIAIVGCKSDLVNQKQVTSEMLAQFCNREKYIGIECSSATGDNIDSLFVNLTQLIIYSPLTTSVSSPVTTKEKDKKRDSKSILNNFKIFNKNSNSKESKESAKDRDGLGSSSSGLSINSTSGGSGSGSSTITNITNGTSHTDGSILSNSGGSSGSGQTKESGSGLNTSSSGTTSTTLGIHMSTTLNASSTLDSSSSSNSSGSGKKKSVFKLKSSTSGDKDNKDKDTKDKDRDRDSSKGITSPKYAVQMSSGGSGISTIGAVETIQHRQASTTPDTSEHFKKKREKILSLPFPHQPPLKDANGRINYYQVCSKEVANILQTPFLLNDPNTKPTTTTTTTTTTTTTTTTTTNITISDRIYDDDNQDDNPYSVANIEDDYSSNNNNSNNNRNIPTPTITTTLSLSNNNNNNNNNHSSTPPPSSNIRVSSSSSSSNNNTPRTPETVAAYKEAYGYNNEEEATGTKSWHKSTLEILPTIATLKHSNQSSGSGNLRITNTDASASASASTPPSSQNPSPNPSPTPDHVQQNFSKAFPSSRDPINRINSSNFNNLPTKTTTTTTTASSSNSNSNNNIDEEEDSFNNRLKPRINNQQHQQHANRNIMRSEPSLVFPGSNIKAAMSLSSNAIFTAANNPTVTTDFSNQFETESIISGYERDDLSEAGIEFEDDKYNSNPILSNMKNAYTINHSNSNDSFVNYKTNHSIRNSNSNSNNSLLNNNNSNNNNSNVNLDDDNLSGELDPYDVQFLSQASQHAINNGSNNSNNNGNIKKKTEMEEKVDEESDLNERFQKVVLRFREFPSLDTNLYRLQEICTDLLHISQDFIHTVKTYGRIIIEERYLKEKTIKSKSIGGHLGGDKYIVRNVLFKFSGGSHVYDEWGGAKVGGQELKGCMATLNCPISGLCVPLMANVDFMGFRLIAMSVLPISSVGDKSTIVYGSSDMGKTVHSDDKALPIMKSLSQMLNLKAHYGGTQKPPVLLYSATDIEGHIGFDGRFYLIDFSRTFPPTYPDDNVQGGHLFQLFRPEFLKGHPKPLCSDAFSGFIKHDPDRSVHHNEVKEASHRLLIELIPRLSNRLKWMIKESLNNGTLLTGDVHIPEQFHRQGINMRWIGHMLLIIDDRDACFVLLIEAIARVLKNDLRRRLREAMKKYNQPLSAPYMDLTTKFLNMVFGSDSELGPEYNSEEFWNVTVQKELASKFYILNIPSVLTNRYNNSNNSSSNSINVDVNSNNNSNNSGTNNVNSSGIINNSGTSINTSGGGNKENRKSLYFNSSNNNSNNINSSNGNTTPPLSSSPPSQIPLQQSQQIVNTWKIRDILNEKLEGTGYPPVLGRHLLFIRFRDMTHLKFRKKTIEKVADTGNNLWNPYKPFDTSDLKKIGVRVKHTDLVTNAEGTFFYAKAMTERSVTTAVHLLHKARKRFELALISNPNNKETLQLCAQTWYKILEFSASQGKNMSNVRFSMSDRVVVNTDRYFLRAIDADPKGPLILFFYANFLARCERYEKAEEFFLRSLEADSNNYRCLTAYANFLTERGFPNESSQFLERAKQCKAILVGGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZF2</accession>
    <name>A0A8J4PZF2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000686</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000013">
        <property type="protein sequence ID" value="KAF2078048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZF2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035310534" evidence="2">
        <location>
            <begin position="22"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="87" mass="10094" checksum="289F90A99C2EF4CF" modified="2022-05-25" version="1">MINRTYLFLVLFLIALSCVAAAASPTSIAQEKIAELEKMREKSKMKRMNTEQFDKLKEKGVKANDRVSEEVIRNLKQKAEEARRNRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZH7</accession>
    <name>A0A8J4PZH7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutamine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glutaminyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_001783</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Gln) + L-glutamine + ATP = L-glutaminyl-tRNA(Gln) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20121"/>
            <dbReference type="Rhea" id="RHEA-COMP:9662"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.18" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000045">
        <property type="protein sequence ID" value="KAF2076881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZH7"/>
    <dbReference type="OrthoDB" id="10250478at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004819">
        <property type="term" value="F:glutamine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006425">
        <property type="term" value="P:glutaminyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1160.10:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.10:FF:000007">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.800.10:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2420:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1290:FF:000002">
        <property type="entry name" value="Glutamine--tRNA ligase cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.10:FF:000011">
        <property type="entry name" value="Glutamine--tRNA ligase cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000037">
        <property type="entry name" value="Glutamine--tRNA ligase cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1290">
        <property type="entry name" value="Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="entry name" value="Ribosomal Protein L25, Chain P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004514">
        <property type="entry name" value="Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007638">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007639">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042558">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_N_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042559">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050132">
        <property type="entry name" value="Gln/Glu-tRNA_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_bL25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_bL25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049437">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00440">
        <property type="entry name" value="glnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097:SF4">
        <property type="entry name" value="GLUTAMINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097">
        <property type="entry name" value="GLUTAMINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20974">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04558">
        <property type="entry name" value="tRNA_synt_1c_R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04557">
        <property type="entry name" value="tRNA_synt_1c_R2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="Ribosomal protein L25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaminyl-tRNA synthetase class Ib non-specific RNA-binding" evidence="14">
        <location>
            <begin position="8"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Glutaminyl-tRNA synthetase class Ib non-specific RNA-binding" evidence="13">
        <location>
            <begin position="168"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="11">
        <location>
            <begin position="243"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib anti-codon binding" evidence="12">
        <location>
            <begin position="551"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA synthetases class I (E and Q) anti-codon binding" evidence="15">
        <location>
            <begin position="661"/>
            <end position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF20974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2076881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="760" mass="86276" checksum="C2D3345E889EFAF6" modified="2022-05-25" version="1">MPPKVVSEELVTMFSNLGLDSAKAKEAAANANLSSQLKDIVVEAGVESGCDKAVGIILYTIATKYPQNALRHRATLCKYIVKKEVSNINLQACLDYLRRTANEELNESDFVSQCGVGVVITRDQVKSAVADYISKNKDEIVEKRYQFPIGSALTDVKNTLKWANTKDIKEEVDEAILALLGPKTDADKAPAPKVKAPAATTATAAVVEEKPKAKEEIRFPDPSDNIQNTPKLLKDHLEATGGKIVTRFPPEPNGYLHIGHAKAMHLDFGYAKKNGGKCYLRFDDTNPEKENQEYIDSIIDSVKWLGHEPCEITYSSQYFDQLYDLAIELIKRGFAYVCHQTGPEMSEGREKMTDSPYRNRSVEENLSLFDDMRKGKFEEGKAVLRMKGDMKHPNPCMRDLVAYRIKYHHHPVSGDKWCIYPSYDYTHCLVDSIENITHSLCTLEFEIRRLSYNWLVDVLGLYRPVVWEYARLNLTHTVLSKRKIITLVQKGFVKGWDDPRLSTLNAFRRKGYTPEAINSLCETIGVTRTNNTTIPYELLELCCRQDLDGKATRAMVVLDPIKVVITNYPQDQTEQITVPNIPANPALGTHQVDLSRELYIERTDFRMVDSKDYFGLAPNKEILLKYAYNIKCNEVITDDKGNVTELRVTYDKDNSNKKVKTIHWVSSVAGQKPTAVEVRLYDHLFKDSEIGDDWLEHINPDSLKVIENAVADKTVANSKEYDRFQFERTGYFCVDKDSTSDKLVFNRTVSLKENKDKKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZK4</accession>
    <name>A0A8J4PZK4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Splicing factor YJU2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_000522</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the spliceosome which catalyzes two sequential transesterification reactions, first the excision of the non-coding intron from pre-mRNA and then the ligation of the coding exons to form the mature mRNA. Plays a role in stabilizing the structure of the spliceosome catalytic core and docking of the branch helix into the active site, producing 5'-exon and lariat intron-3'-intermediates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the spliceosome. Present in the activated B complex, the catalytically activated B* complex which catalyzes the branching, the catalytic step 1 C complex catalyzing the exon ligation, and the postcatalytic P complex containing the ligated exons (mRNA) and the excised lariat intron.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CWC16 family. YJU2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000010">
        <property type="protein sequence ID" value="KAF2078138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZK4"/>
    <dbReference type="OrthoDB" id="674963at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000349">
        <property type="term" value="P:generation of catalytic spliceosome for first transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03226">
        <property type="entry name" value="YJU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007590">
        <property type="entry name" value="Saf4/Yju2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043701">
        <property type="entry name" value="Yju2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12111">
        <property type="entry name" value="SPLICING FACTOR YJU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12111:SF1">
        <property type="entry name" value="SPLICING FACTOR YJU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04502">
        <property type="entry name" value="Saf4_Yju2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="199"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="209"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="242"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2078138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="313" mass="35928" checksum="D10BA415740AB165" modified="2022-05-25" version="1">MSDRKVLTKYYPPEFDPSKITKIKVKKLPYTKVTTMLPMSIRCNTCGEYIGRGTKFNAKKETVAGESYLGLKIYRFYLRCKKCAAELTIRTDPKNSEYICEAGATKNYEPWKETQDEKDTRLHQEAEEEVDAMKALENRTLQSKREMEILDGLEEMKAHNSRLAEIADTDILLEYNLKKQQEIKDQFEKEDEDLIKQVFGKNKDHDSQDQDEDEDEEQDSEEDTKIKRIDDEQDDKFNSLFNSKSTNDNNSSVNNNSTKTTTTTASKPKTSILSNVKIVKSQEPPKPAPLAQPNTNLSSLLGGYDSDEEEEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZL1</accession>
    <name>A0A8J4PZL1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mediator complex subunit 27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_003494</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000110">
        <property type="protein sequence ID" value="KAF2075189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZL1"/>
    <dbReference type="OrthoDB" id="1868004at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021627">
        <property type="entry name" value="Mediator_Med27"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13130">
        <property type="entry name" value="34 KDA TRANSCRIPTIONAL CO-ACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13130:SF4">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11571">
        <property type="entry name" value="Med27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="424"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="17"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="427"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2075189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="456" mass="52691" checksum="41DEB5BBD8561845" modified="2022-05-25" version="1">MQAQPHQQTNVNTLTPQQQQQQLLQQQQAQQQQQQQQQQQQQQQQQQQQQQQQQQQQQLQQTQPANLLTPQQQKLLLEQYDEVISQVLYLRSNVSDFLNGLIEDVKNNNLTQPNKELMKKKLKSSMEGALNAVNVIEKLTQRISTYTHPIKSSFFLQEPLWAMSTGLYDNQNFKAQIQLRLENYWRYQTSVKSSYATNVLENKLLETFPEIMDEQEEMTLSKKRKQMTPTISPPILFKPPLPLSSISSSPYDYFSNIDRIIHSQRQESALDIQKIQKMQSNSYPKGLLVECSDVFKCLICFGVLNENKECFSIDRISFFGVKENFDSFWVSSKYNVFKRISANALEAISYYSVHSSGSVLKSILSWIWSFRGLFIEECKGCQNILLLDSTEYLYLPPSLRTFDGNFTPYHPICYQNTLHKDTHNHNHSSTTTNTTATSSTSNTNTTNASTPGMNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0M0</accession>
    <name>A0A8J4Q0M0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein transport protein SEC31</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Protein transport protein sec31</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">CYY_001127</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">COPII-coated vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat SEC31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000025">
        <property type="protein sequence ID" value="KAF2077586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0M0"/>
    <dbReference type="OrthoDB" id="542917at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007029">
        <property type="term" value="P:endoplasmic reticulum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000526">
        <property type="entry name" value="Protein transport protein SEC31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.940.10">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024298">
        <property type="entry name" value="Sec16_Sec23-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040251">
        <property type="entry name" value="SEC31-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13923">
        <property type="entry name" value="SEC31-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13923:SF11">
        <property type="entry name" value="SECRETORY 31, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12931">
        <property type="entry name" value="TPR_Sec16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="7 15" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="118"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="173"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="257"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Sec16 Sec23-binding" evidence="17">
        <location>
            <begin position="586"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="396"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="829"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="924"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1089"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="402"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="837"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="924"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="934"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="957"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1010"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="1039"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1089"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1117"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1146"/>
            <end position="1159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF12931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF2077586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1266" mass="138397" checksum="77BECC6CF3304BE9" modified="2022-05-25" version="1">MSKLKEIQRQATVSWSPIASIPGYLVAGTIAGTIGVDFDTSSTLEIYSLDITNESKQMTLKGQINVNTRFNKVAWGNASANYPQGIIAGAMDNGSINLWNPIKILEGASSEECLIGVGQKHSGPVLSLDFSWDNPNILASGGSDSEIFIWDLSNPSQPSAYSPGAKPQQLGDVTSVAWNKKVSHILASANNHNIIVWDLRSKRSLLSFTDRNRKCSYRSIVWHPDEPTIIAATSEDDEHPVIQTWDLRNTTSPAKVFEGHRRGVWGLSWCPNDSKLLLSTGKDNRTLCWNYDKEEILCEVESSTDPNAWNFEVQWSPRIPALISTSSFGDKINIYSIQDVNQRSASANTKTNALGIQEQQQQKQPISFDHTPNWLLRPVGAAFGFGGKLVVFGRPSKGTKATSPSTGNSPSNSVTAPVPPKRTVNIVKVTTEPELLKDSEELEKVVNSGAYEQFCEEKIQSSQSEEEKAIWGLIKAKFSQDDRLKILNYLGYDIEQIKKELSVFLGTLPELPIEAKEEDVVVEETTTTEETAAKSPEQQQNINGEDFFNQDFSEQLAKTTESSGASTIKRSSSPIQFPVDGNEQMITKALLIGDHENAVDCCLRLGRFSDALILAHSAGPELWKKTQQAYFAIVKSPFGKVVSCIVKRDFETLIKTADLADWKASLSILCTYATPSVFKQLSGVLGDRLDNEAKDIKSATLCYICSGDIDKTVNVWTRSQTQETHKDLQNLIEKVSIFRSACNNNNGSLNEVLSIKYAKYAEILASQGNLAASLRYLSFVNIPACQKSCGVLYDRVYRSTNNHQGIAPPPFPFQQVDVVPSSPMRANPTQTTATAGNPMQRQPQPQNNMGPMNPMNNVGPMNPMNNNNNRSPFSPQNNMGPMNPMNNVGPMNPMNNVGMMNNNNRQPFSPPQQSGPMNPNIPMGPGPMNPMGPGPMNNQMNRPMGPPPTGSGSMTSAPPPMGQPPMGNPMNPMGPPSMGQPPMNPMGPPPPSMGQPPMGNPMSPPPTGPMNPMNPMNPMNPMNPMNPMNPVNPMNPMNPMGPPPSMGQPPMGNPMSPPPTGPMNPMNPMNPMNPMNPVNPMNPMNPMNPMNPMNPISSPMPTAPPPMGPGSVEANPSPASFRSPNLASKDSKPADFTTPLPKPNVHNPSPANANASSEPLSEDSVQFIEKLNRSLENLKDKSINNRQLWEDANKRCQQLIQKVTNKLISNEALKALEIVINAIVENDFKKANEKYIVITSTPFWNEIGSQSMVGVKRLIDLGLKYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0N9</accession>
    <name>A0A8J4Q0N9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">T4 RNA ligase 1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000055">
        <property type="protein sequence ID" value="KAF2076609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0N9"/>
    <dbReference type="OrthoDB" id="16479at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019039">
        <property type="entry name" value="T4-Rnl1-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09511">
        <property type="entry name" value="RNA_lig_T4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T4 RNA ligase 1-like N-terminal" evidence="1">
        <location>
            <begin position="76"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="440" mass="50694" checksum="FE7F3C2371D9C627" modified="2022-05-25" version="1">MINNVESYEIRNSKMFALNITVQDCRDATKGVAGFRETCKNGIICFNYDFCHRDTFPDPELEKDAQKAFLLKVRRECRGILFEQATGKLLCRKLHKFFNVNEMDETHPSKIDLNEPFQLLEKLDGSLIAPFYYGDKVCWGSKSGPTDLSDKVERYIERMLTPADTTTAPIKFNEFSKHWLTRGFTPLYEWCSESNQIVLYYATDKLSLISLRNMSTGEYMDVPTMKQTCLEYNVPMVNTIDIESHPVFSSPYKNKNTQELLTCVQKMEGVEGYILKFESGKIYKLKSEWYFGLSHAGPSTRFTHEKDVWALILSGTLDDTIAKIASIGPIPERYKNIQSFSVTLYQRIEQVSIKAIESTIKAKRDGKQRRHITNDPTIDTQSKKILFLFYDRISELDSVQDHLSDVSSHIIDKIKVLCNNSSKLEEARNILGKDIKYKDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0W1</accession>
    <name>A0A8J4Q0W1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">2-(3-amino-3-carboxypropyl)histidine synthase subunit 1</fullName>
            <ecNumber evidence="4">2.5.1.108</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Diphthamide biosynthesis protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Diphtheria toxin resistance protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">S-adenosyl-L-methionine:L-histidine 3-amino-3-carboxypropyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_002646</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-histidyl-[translation elongation factor 2] + S-adenosyl-L-methionine = 2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36783"/>
            <dbReference type="Rhea" id="RHEA-COMP:9748"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="EC" id="2.5.1.108"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DPH1/DPH2 family. DPH1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.108" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000075">
        <property type="protein sequence ID" value="KAF2076032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0W1"/>
    <dbReference type="OrthoDB" id="1649088at2759"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11840:FF:000001">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11850:FF:000002">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11860:FF:000002">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11840">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11850">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042263">
        <property type="entry name" value="DPH1/DPH2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042264">
        <property type="entry name" value="DPH1/DPH2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762:SF1">
        <property type="entry name" value="2-(3-AMINO-3-CARBOXYPROPYL)HISTIDINE SYNTHASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01121">
        <property type="entry name" value="Diphthamide_biosynthesis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2076032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="461" mass="51769" checksum="4A4858F01819F1F4" modified="2022-05-25" version="1">MSNVEENNNTVDKVENNDDDSNTTTATPTKTATGSVTLKRRFVGKKKIAAAAASGATTESTSKDIVPSEKKTTTTNGVKVFGRGLAMAAQQVPEDILNDVELNNALKVLPSNYNFEIFKTIHRLRLNQSKRVALQFPEGLLMYSCLISDILEKFAGVETIIMGDVTYGACCVDDYTARSLGADFMVHYGHSCLVPIDVSEIKMLYVFVDIQFDLKHFIDTLKFNFAVNTRMVLVSTIQFSASLQSARSTLSEHFKYVSIPQEKPLSHGEILGCTSPKITTPQADQGDEASEEIIVYLGDGRFHLESIMISNPHIKAYRYDPYAKVFSIEKYDFDEMYRMRQEAIDQARDKKKYGIILGTLGRQGSPKILEHLEELLKIKGKDYTTVLLSEIFPAKLELFSDVEVWIQIACPRLSIDWGYAFKTPLLNPYEAEVCLGGIEWQSIYPMDFYSKTGGKWTNYSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1A2</accession>
    <name>A0A8J4Q1A2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="15">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_001784</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="15"/>
    <dbReference type="EMBL" id="AJWJ01000045">
        <property type="protein sequence ID" value="KAF2076882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1A2"/>
    <dbReference type="OrthoDB" id="3352408at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02081">
        <property type="entry name" value="P-type_ATPase_Ca_PMCA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000036">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000039">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000029">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000018">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006408">
        <property type="entry name" value="P-type_ATPase_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01517">
        <property type="entry name" value="ATPase-IIB_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF425">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 15" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 15" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="12 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="308"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="725"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="802"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="841"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="879"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="912"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="16">
        <location>
            <begin position="28"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2076882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1021" mass="114141" checksum="D4265D05EA4A6166" modified="2022-05-25" version="1">MLNIDNSVTVDDLSSIVANENLQLINERYGGVSNLVDRLGSNRENGLTNQEASNPARVEMFGRNKMDEIAQKSFLFFVWEAIHDKTLVILIVSAVVSIILGLTVEDPKTGWIDGTAILLAVLIVVMVTAGNDYNKEKKFRKLNSIRNERNVSTIRSGRVSPISVYDLVVGDIVKLETGDTIPADGIYLSGQGLSVDESSMTGESDSKRKSNTEPFLLSGCQVLEGSATMVVVAVGMHSQWGKLKLLLQSPNSDTPLTQKLEKLAEVIGKFGLVAAILVFAVLLLKYIIHFTTTHQKWQWSELGTIVGFIVTAITIIVVAVPEGLPLAVTISLAYSMIKMMKDNNLVRHLEACETMGGATNICSDKTGTLTQNRMTVIRKCIGKVNHDYSEEQKDNLLLESPDFLTLLSEAISLNSTAYIEKHQDRLNDHIGSKTECALLEWLDKIPNQSYDVVRNENKSRLVRTYPFSSEKKMSAALLKNGNKFRVYAKGAAEIVVSQCSSIITERDPIKMTKEEKLLLHKEIEIYASDGLRTLVLAYKDIEDQHIGNDLSEDIIYSDLTFICLVGIKDPIRKEVPKAVKKCQNAGIFVRMLTGDNILTAKNIARECNILREGGIAIEGPQFRQLTDNQLDSIIPHLQVIARCSPTDKHRLVHRLRELGEVVAVTGDGVNDAPQLKEADVGFSMGIAGTEVAKEASDIVLLDDNFNSIARAVLWGRNVYDSIRKFIQFQLTVNIVAVLIAFVGSITNGESPLRPVQLLWVNLIMDTLGALALATEPPTEELFNRLPYGRYDSLITYRMWRNIIGQAIYQISFLMAILYGAETLTTLFDLPALSAWTPQDKTTYHTIIFNTFVFCQFFNEINSRVLTNDLNVFRGIHKCYIFLGVLFFSIGVQILLVEFGGEFFGTRHLDYKQWFFCIIIGSGGLIWGFILRLIPIKDKQIQQRPPAKAELLDEIPGINYDIERQPLLGPKSNWRKAQNIFKKINVISAFRNQPRLKRTKALFSTKNSNNQTGLRRQSAPRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1D4</accession>
    <name>A0A8J4Q1D4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002638</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000074">
        <property type="protein sequence ID" value="KAF2076074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1D4"/>
    <dbReference type="OrthoDB" id="16880at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="139" mass="14950" checksum="9ADBAD2460F77DE8" modified="2022-05-25" version="1">MSDAEYSPPSVRRLTENFQGPSSTSPTNSPSVTKKTPTTTTPKQPGTFDNDTSSISKQNSAAKVERFLKEEVLPKGHAPGSAHRSALNKPAPNELDNKARDVNKFSGNSHPVYGLMLAGKQHDTSTTKKKIVIPPSSAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1M2</accession>
    <name>A0A8J4Q1M2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002432</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000067">
        <property type="protein sequence ID" value="KAF2076254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1M2"/>
    <dbReference type="OrthoDB" id="31075at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035253781" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="660" mass="74979" checksum="65496CD8F3461C9C" modified="2022-05-25" version="1">MKGYFLAFLFLVIIINITSVVSISTPIQHNVNTKISTSSIQNTINELYKEIQNYQNKKDKSYIPMVQWAEVKGLFQSDVRVNFVGNELKTEARRKFAIPDSNMFVTNFVVFTLLESHKLGTIDLDPLVLQDAITAILSFKDKNFINHDTAAVYTFWPQIFNSTLNVYSQYPMNLNHLILQYGSFQKFVDFFAEEFKLLNVLKDMQMIAEALDTVSMAFHIPSDADDSGCNLGLGQVLVNLPNSSQFASTIATWKQSNANITTLFDTYSKYAYRPFSPILDQNSIDPRSYYVLQDFLTSWKESNRDLQSLLLPTTWFESMSEQKNTFPDLQMPFNVNNVDFSVATNTLFGIASALYQLQLEAGNSDILDEIFTQDIQNLCLSTAYLIENVVTSQIVYNRSDLALLYYPSIYDFTWFVSRTVFFLQEFELAASNSNDIVFPNTLIQVLSILNPVVENLMTNQLVERLNVDAKSGGTYWDDFLGDGDTLFGKPEKYGEDRFFSTSLAINALIDTWTLPVANSVSARVWKSNTPDMIQTIIKSAVSYVNQFILDNPEQQYNAFFSGSVKGTDTLDFFYPANLCLFFNGTICNPHYDYENQFELANAVSGYIDSATYASMVQESWGNITTPTIFYGYNNASFPFWSSKPLTYSISILALSKYITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1Z1</accession>
    <name>A0A8J4Q1Z1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005880</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000246">
        <property type="protein sequence ID" value="KAF2072806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1Z1"/>
    <dbReference type="OrthoDB" id="2414723at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045068">
        <property type="entry name" value="BACURD1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING ADAPTER FOR CUL3-MEDIATED RHOA DEGRADATION PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145:SF8">
        <property type="entry name" value="RE57120P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="89"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="177"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="345" mass="38882" checksum="9BF63FB4910BDA6F" modified="2022-05-25" version="1">MNTTKVKYTVDCLERQYKADLDALIREIENDRDPKVLIHIATSFGAQFSSGLSSIRAEIYSLDSVIVREKIAGASRDTASSNSSNSGNDIIKLNIGGVLFETTRTTLNQSPFFNKVFSGEIKVQKSEGNSYFIDRDGTHFNYVLAYLRDGSNEIPESIKNQVKKEMVYYQIGFLDSQIGNLDTFKIINDWIDSTKQFKFELLYRASENEFEASTFHEKCDGQGATITLIKTTDGNIFGGYNSQSWTSNPNSNGDNKCFIFTLVNKHGIKPTKYLANPEKERFIYSNRQSGPGFGTDDISLLKQDTSYQQFPSSAYIDTTSQGEKTLTPSDSFILQEYEVFKVLEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q264</accession>
    <name>A0A8J4Q264_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001365</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000031">
        <property type="protein sequence ID" value="KAF2077362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q264"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044614">
        <property type="term" value="C:nuclear pore cytoplasmic filaments"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034398">
        <property type="term" value="P:telomere tethering at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037665">
        <property type="entry name" value="Nucleoporin_S59-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198:SF6">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP98-NUP96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198">
        <property type="entry name" value="NUCLEOPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21240">
        <property type="entry name" value="Nup98_GLEBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="164" mass="17945" checksum="65CAD6244898C238" modified="2022-05-25" version="1">MHQMLLSLVNQLQLDYLVQVGYLLPIYPHKVRPVGTGAPPYTATESEGCSIVTITAMDAYSTKSFEELRLEDVLYKKDKIYKTGATTTTSPFSTAFGGASTPLDGTSSPFGTSSTTSSPFGGASTTSSFSRASNGPNHINIIINKCTNFNWNIWLIINYTKHFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2B3</accession>
    <name>A0A8J4Q2B3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exocyst complex component 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_001142</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the exocyst complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the SEC5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000026">
        <property type="protein sequence ID" value="KAF2077524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2B3"/>
    <dbReference type="OrthoDB" id="26242at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029175">
        <property type="entry name" value="EXOC2/Sec5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039481">
        <property type="entry name" value="EXOC2/Sec5_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13043:SF1">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13043">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15469">
        <property type="entry name" value="Sec5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component EXOC2/Sec5 N-terminal" evidence="6">
        <location>
            <begin position="325"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="562"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="731"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="735"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="749"/>
            <end position="758"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU365069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF15469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2077524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="991" mass="113784" checksum="0705A2BB0EAB3EEA" modified="2022-05-25" version="1">MSSDSSSGSDSYSDYYDDEDDYLETEPTQWSRLKDGWQEFIEDESAFFVVQNESGQTTGDSGQIDDRDRFYDPLGILKMPPRKSQSIKKELLSLESEDFSPVVFLSEIHSQTKFTELSVGMSKLKEALSSKALEIKFLVKDNFEHFVKCKDTVDEVFDLVSNSKMLEDMNTSFTKIIDKSSLVYHPLLRGKQEADHIRRVLTLLNKFKVIFKLPGKIQENIRQGEFEKVVHNYRNAKMLITSNNQKKAFQKILYDIEKIIEDFRTQLFTILRDPQSKPDLLKKSIKVLMEIGNGKNEWSMIGDPCWYFLSNRNNAITTLIQQCSNDKTLPNYKIIRKLSILLLSNVPNLFKMGKSYIEGKFDDNNEYLKMKQQQSILQQQQQQQQQQQQQGDKKKELKTVSVIVYFIDESFSSYRLDQEKKVGDLVELCKKRFIETEEDQFCLYKLSEKKPKDGGVTTIKLVELALDESPFKLQKKWIDKSSSKTHKFMFKKKGEDYSTKITTLDSVHVVQKDLTSSYKSHKKQISAATSAINEENFKKLVVGLLELYSQKVEELFFDEDFNSPVPGYDEEEDDGTPDTSSNMVDNVNEVMKCQEMLHNLGMDEYYLRSINKLVESLTLHFISQICSEMVAEVSFLYLLEDWSINEFDDVISTNQQVGGMTTTRLLGEFVETIKSNLSKLSFLANKPMLLSPVESALCEAIESFGDCLHRMAFEQEQLDLSNIYNLDNHHHHHHHDGDNASDDGRSDVDGQTSSGKDKNTFTSNQKLLLILSNCSTVVSKTAVQLRSFYVSLFHQPFTSYIKKVIEKLGVLERLILDKYVHEKTKSLGEYITRGILHSGNIWMDKIHPHKVSDYILTILTKLVFVHNEVMKTINSIDVSSAVVSRVFEYLLVTIRHQFSFVEPTLISTEGKSQFLLDVLFVENILQSFGSEKTVKLSQLIRKDLSFTPATSSGGMSGAFNPAMVTKNLEQILQRNLLKTTLLFSCFTQSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3E1</accession>
    <name>A0A8J4Q3E1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000169</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000003">
        <property type="protein sequence ID" value="KAF2078545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3E1"/>
    <dbReference type="OrthoDB" id="408117at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="3"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="39"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="140" mass="15924" checksum="BD1163B0D03F1CB0" modified="2022-05-25" version="1">MAYVSKEITKFLRDCDKDGDNRVTQAEVLAYYNSKDPKSAQKLTNQLFKKYDKDRSGDLNIDEITKYAKGLLTREISKVLKQFDTNKDKKITYAELISNCEDGELVALMTFGDLNKNNDDVVTVDEIRAYLNSHGSGPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4E5</accession>
    <name>A0A8J4Q4E5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">DNA-directed DNA polymerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_000005</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000001">
        <property type="protein sequence ID" value="KAF2078634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="275278at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08640">
        <property type="entry name" value="DNA_pol_A_plastid_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06139">
        <property type="entry name" value="DNA_polA_I_Ecoli_like_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09898">
        <property type="entry name" value="H3TH_53EXO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09859">
        <property type="entry name" value="PIN_53EXO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000034">
        <property type="entry name" value="DNA polymerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000051">
        <property type="entry name" value="DNA polymerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.10">
        <property type="entry name" value="Taq DNA Polymerase, Chain T, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020046">
        <property type="entry name" value="5-3_exonucl_a-hlix_arch_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002421">
        <property type="entry name" value="5-3_exonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001098">
        <property type="entry name" value="DNA-dir_DNA_pol_A_palm_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020045">
        <property type="entry name" value="DNA_polI_H3TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002298">
        <property type="entry name" value="DNA_polymerase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10133">
        <property type="entry name" value="DNA POLYMERASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10133:SF27">
        <property type="entry name" value="DNA POLYMERASE NU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01367">
        <property type="entry name" value="5_3_exonuc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02739">
        <property type="entry name" value="5_3_exonuc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00476">
        <property type="entry name" value="DNA_pol_A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00868">
        <property type="entry name" value="DNAPOLI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00475">
        <property type="entry name" value="53EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00482">
        <property type="entry name" value="POLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="5'-3' exonuclease" evidence="12">
        <location>
            <begin position="257"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family A palm" evidence="13">
        <location>
            <begin position="1072"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="48"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="551"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="49"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="551"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2078634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1337" mass="151857" checksum="F1F2BF4AFC2D43F9" modified="2022-05-25" version="1">MIYVSTSHRLIGLGKQLITHNSIVVGGTINSIHHHQQQQQQRYSYLTISNNNNDNVDGNDSVDSTSNNTTTPTPTKSRVKIKIKSKDPFILLPNNKKVYLKSTKIQKTKLNIHNDLGGGVIDGAATAAQPILDTSLSRLQNNIVLNHPSHPSIHHHHHDNFYQHPHINVNIPTPSTTTTTTTTTTSPPKLKITEKRKVKKEKVDLNINKTTASLDSITKETVTTTTTTTTIAKETLIPIENLINNSNSNNNIDHVVKKGDSIVLIDGTLMFYKGYATTTKTMGTDVNGVYAFTQSLIKVIKELKPDYLVVCFDVFGGKGNFRREIYPQYKGNRPAPPPELVKQIPLLPQVTQALGIPFVQLDGYECDDLLASYAKQSVEKGLKVTIVSEDKDLFQLVRPNVNIYSVRNTEMYNEQDVIYKYGFPSSLFVTYQSLLGDKVDNIPGIPSIGKKAATDIVKSLGHLNNILENVDQIQVLKHAKLIEEYKDQLKMNYQLAKLNENVVLPVSIENSFFNPINRANFVKFLEKFEFKSILNNLHNLKLKFEKEEESSINNNNNNNNNNNADESATPSREYSKVEFNYMDENIILDPISNEEIDQLKPKNVTMVRNVEQAKRVVEKLLSLKNVFHACDTEVVDLDLKKESPIGHGKVICFSIYCGPDIDFGNGPRIWVDVMDDIQGKEILEVFRPYFEDEQILKVWHNYGFDRHILNNHDINVVGFGGDTIHMARLWDASRTTNGSGYSLEALSKELLENPKITIKDLFGSKKIKRDGEEGKAIVVPPLEFIQRDKKFIQPWIEYSSLDAELTWKLRENLHLKLLDMEWTPGTNMWDFYYLMWRPFGHLLTEMEKRGMKIDIPYLKTLEEKAQNDIEENRKRFYEWAKKHSPGAEYMNSDSDAQIQQLLFAPVVNKKTKEALPREKDFECDNVLNVIEPGKTKAKKKMKFFLHGIGLESKTVTASGWPSVDSQSLRELAGSNPEAGKYGSAFKFFVDKYPEDKERGESEGREACLAINSLLEIGSIGTLINSFILPLQKLADSNSRLHTSVNLNTETGRLSSRRPNLQNQPALEKDRYKIRAAFTCEPGNTLIVADYGQLELRLLAHITNCKSMINAFHVGGDFHSRTAMGMYPHVKEAIDKGEVLLEWDGDGEPPKPLLKNTYASERRKAKTLNFSIAYGKTPHGLSQDWGVTLKEAQETLNRWYEDRPEVLVWQRRTIETAHRHKWTRTLMGRYRHLPDIDNNAKGMKNHAERASINTPLQGGAADIVMKAMLIIEENTRLKELGFKMIMQIHDELILEGPEENADEARELVVKLMSNPLTTPLLIDLIVDCRYAKTWYDAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q507</accession>
    <name>A0A8J4Q507_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Srp40 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000299</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000005">
        <property type="protein sequence ID" value="KAF2078432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q507"/>
    <dbReference type="OrthoDB" id="31137at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007718">
        <property type="entry name" value="Srp40_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05022">
        <property type="entry name" value="SRP40_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Srp40 C-terminal" evidence="2">
        <location>
            <begin position="368"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="128"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="157"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="343"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="442" mass="49212" checksum="C8EA462A69B4787E" modified="2022-05-25" version="1">MAIKDNSKKVDLKRKKEDEEVEIEKSKKLKKEEESDSDSSSSSDSESSSSSSSSSSSDSDSSSSSSESEKEEKKVDSDSSDSDSSDSESEKEEKKKVESDSDSSSSSSSDSDSSDSESEKEEEKKKVVSSSDSDSDSDSSSSESESEKEDKKKVESDSSSSDSDSSSSSSDSSDSESEKEEKKVESDSDSSSSSSSDSESEKEEEKKVDSDSSDSDSSDSESEKEEKKKSDSDSSSSSSDSDSSDSESEKEEEKTKKSDDSSDSESEPETKKPVKKEQPKKKEEPKGKKQQEQQEEEEEIKEPVKQNGYNANYGDSYAMDTNEDYQEEENYNHYNKNNKKNNRFNNKQPQQQQQEQATDVNGKKQNAPFRRVIAENVVFDRHMKTAQDVYTDVWGERVNNSMKRVIGEGFRKAKNKMKRGFSHSTITEDISCYKIASDDDTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q866</accession>
    <name>A0A8J4Q866_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_002173</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probable methyltransferase.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000058">
        <property type="protein sequence ID" value="KAF2076496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q866"/>
    <dbReference type="OrthoDB" id="20143at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050869">
        <property type="entry name" value="H3K4_H4K5_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197:SF251">
        <property type="entry name" value="EG:BACR7C10.4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="6">
        <location>
            <begin position="43"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="MYND-type" evidence="7">
        <location>
            <begin position="87"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2076496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="512" mass="58880" checksum="6C7CE136E98C01CC" modified="2022-05-25" version="1">MLCVGSPLTELDEVEVKSSALDKRVACIQELTKECGFGDEGKWKLKVDKSAIHGVGVFACKDFKVGDEILLAEPFVHLADMSKEDRCDGCWKTSKSVRLTLCKRCKFVSYCSKACEAKMSALHKLECEKMLAYSRTILFQDGHFQRVVMIARMFFIEQMGKRENGCLELIKQLDFGDEPKSKDYVSNVKQMSYNMEYYMDDDVTYDWQKILPICDKFQSSSFLLEQDQSRMLTPLVNLFNHACDPNAYRKRLDGQKLSIIAIKDIKKGDEIRFSYVRGYDKKVTFKALKDGAFTFCGCADCSIIELTDSKDKYYRCKDTNCNGRVDVRLCSNLPVNKNYGTYHAICLKCSSIYDADKLKKKGDVIKLNLSEKFPFVARNVQELMLRLYFEHFHDLDSHLLMLFTTVDLLSVSDRNIIKRIELAALARYGFYSERVYSIYCSIFVRLITNPATVPEGMYFLRRLETIGTTLKCGNDDPSFSQMKTNVKKRYARSVSIDNILPSPDIYFDRSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UP77</accession>
    <name>A0A8J4UP77_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009871</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000837">
        <property type="protein sequence ID" value="KAF2068806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UP77"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="329"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="815" mass="94184" checksum="1810DB6BBFF07314" modified="2022-05-25" version="1" fragment="single">MVIYVHWISPSFDKGWKTEVNSRKHFTLKIVLDESGRVDKEKLNEIPEGTSSIRIEIIASLSLRSQRDHLHRYIDVDKGFIPSSVTRVEVTCDFEQENYHASDGWLPDSVTMLTVRSLPFPATLVPKNIRYLKVEQQEKAIEIDMVPSTTEYLWIGGLSKNGGINLHSIPNTVKELVVDFDVLNKIQIKPTFTSPIHISNPRMYHDGDIFADYEPNKTLCYLNIQTITSSKPIVAPLPTITSLVIEEDGYFNNNLSALHPLPPQIKYLSLPYTYNQPIYQQQYLPISLTHLELSVIKDQNNHFTNNWLCRKGYLPPSITHLKINIYFEEDDDEQDDNDNDGGNDNVKIEEEERNVDKELNNDKDDEIEIDSNSTHPLKKTKIDQNDGDQVLLVTKQDEYNGEEEGYDPLPSSLKILIPSSIQSVCFNIDHKSYRSHVTVLDKDDYYSTTPTPPKIRQGLKELVLPESFNNHIHNSTLPSTLQSLSINNPDYTNPLNSNNLPLSLTSFKCNSDKIKLDLSLLESIKELKVNADAITYYPPNVEALELRSPFLEKGYQFQFNKHLIKSLVYSCDIRNPSYGRSYLKELLELLPNVQFLSSEYLDFEVPASVKEFKCLDNLFRCTKARPISSRPFETIHLDYGYYLHFHSQRQDNHQRTEKIIYKHKANQQPMLNSFFKIWRHNYLKPLVLEALYDQTKSLLRFDSETGTLEHIDKFKQYKIFVDYHTQLSPALLKFDKADEVYIYNTTNTNLSNFISKSIKKLRCRFTKEIPDWITHLNLGDIRFSNHEVGNIPPSVTNLTIDTNDEKYIGKIPSTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQ50</accession>
    <name>A0A8J4UQ50_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_008491</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000528">
        <property type="protein sequence ID" value="KAF2070186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQ50"/>
    <dbReference type="OrthoDB" id="10253125at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000002">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000004">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_uS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_uS5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005711">
        <property type="entry name" value="Ribosomal_uS5_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_uS5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_uS5_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01020">
        <property type="entry name" value="uS5_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718:SF4">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="RIBOSOMAL S SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="S5 DRBM" evidence="9">
        <location>
            <begin position="151"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="62"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2070186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="327" mass="35697" checksum="A48AE0BF606218B8" modified="2022-05-25" version="1">MSFRCSTSRNDIFFSTNWTESLLLNSIQCAINEDIICELISFLCVREVPSYKTLIRRSMADTTAPVAQTADQKTGGRSFGGRPQQRGGPQRGGQRGAPQRGGRSGPSTQAWVPVTKLGRLVEAGKIKSIHEIYLFSLPIKEFQIIDKFLNLKDEVMKIVPVQKQTRAGQRTRFKAFVVVGDHNGHVGLGVKCAKEVATAISGAIVNAKLSVIPVRRGYWGNKIGAPHTVPTKVTGKCGSVAVRLVPAPRGTGIVAARVPKKLLQYAGIEDVYTSSRGKTRTMGNFVMATFFAITKSYAFLTPDLWKAVALTQTPYQAHSAFLAEKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4URA3</accession>
    <name>A0A8J4URA3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006815</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000331">
        <property type="protein sequence ID" value="KAF2071866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4URA3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="734" mass="86083" checksum="6B721F377587BE36" modified="2022-05-25" version="1">MQLFDIVNTKSIPLLKYKINRFKSIIDNNNNNNDNDNDKNKFQYRDWIDLNTIESRDDEKSIQNIDLSHLHQEIQDFSQYLLNYHTKENEALKKDIVQKVIRDFNNDLNLCTTLSPMLRLGLNISKNMSTDVFRLLVDNGDIQILPSQYWTLLSPQHVTFFTNNRDYFRKIYGWNDKDFLKTLLEWQESPLDVDLLKWILLVSDRFNFDLYKQRNPVSQKKSALLLGCRVVLKRKPIFSNKSLFCNSEVFKFLADFYPDWLLGQIKNSTHFLQDLATAQLVIPFLQRVNFRNCFFRTCSQDLNLFKYLDQNQFPYDGPNILSNNENVDYLIKKNMAIFSNSFIFSQNYGVNVDPYSVLKRLISEKNYQDYSRPNSFSNAIKSGNFDYIKFIMQGKHYAAFGNNTWDYFIYLSYLHNETIFQYLLSHSFLLDQTSTQQQEPAPATSFYPNTSFFELICVALSKYDCKYLVWFFKQLTKEKQVEAVQKNIVPSYSYLAFAKKSQKKAAIENVEFLKAKYLEFGSPIEGMFGNLSWEFLFIQDPVFFNQLNLGPTIQKLYRVDKILLLKGAIVARNLSMVKIILNHLSVNGELDSSINLKSILLEYFMAASKSNVGFKVAIYLFEKYPDQFTCNTLDQLVSIAIQKELYILLDILLHRYAIRLIKPESPQHEYEMLKRAYYNLTLSQFKAKQKYLLLYPFTLESLDNDESPPTIVNVIIPKNKTPVAIPFNTDVISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UST4</accession>
    <name>A0A8J4UST4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_004703</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000171">
        <property type="protein sequence ID" value="KAF2073996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UST4"/>
    <dbReference type="OrthoDB" id="8693905at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06627">
        <property type="entry name" value="STKc_Cdc7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000946">
        <property type="entry name" value="Probable serine/threonine-protein kinase DDB_G0284251"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF26">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DDB_G0283821-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="41"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2073996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="383" mass="42181" checksum="6456EB1C30A85B7F" modified="2022-05-25" version="1">MSELSKSADCITQSPKNLAGKKDHPTIPEPRRKLVVKIGDYNLGDKIGKGAFGQVYKGFNVKTGDFVAIKQIEKSRIDNNTLQSVKKEFDILLNLKHNNIVKVLGVVETSSKINFILEYVENGSLRDIIDKFGPLSEDLAISYLYQLLQGLVYLHANRVIHRDIKSSNILITKDGTIKLADFGVASQLSDQAQMTYSVVGTPYWMSPESIQMSGQTSASDIWSLACTMIELVSGSPPYFSLQPMAAMFRIVQDPHPPYPANLSEEFQSFLNTTFEKDPLKRPSAAQLLLHPIFKKHQQNNITIPTLSELTSTLKTLNTKQHHTRSRASLFSINWGDISSSNESTSSGVAATASAMNISAVPSKTTNGVPTSAISKIYSKINAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UT87</accession>
    <name>A0A8J4UT87_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004127</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000140">
        <property type="protein sequence ID" value="KAF2074581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UT87"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="385"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="392"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="644" mass="76099" checksum="200D41B86EAC9C72" modified="2022-05-25" version="1">MDGDDISSIISTLSKLITTYQLNIDDQIQTIINYTKELTNEYMEVVDKFLIISTLSRQITKYQLNNDQIQNIINYTKQLLMYNNKGRNDIIIIISTLPEQITEYQLNNDQIQNIINYTKQLINPFMSGDHILLIISTLSRQITKYQLNNDQIQNIINYTKQLINEKMNVLVISKIISTLSEQITEYQLNNDQIQNIINYTKQLINERMNRDDFRYNISTIISILSKQITSYQLNNDQIQNIINHTKQLINENMYSDQISSIISNLSEQISQDPSNFKECFKNNIILKTCYHLDFRYSYSNILDLYSNNPLAFEHSYISILSLNQIIELDQFLKFLEIMIACNSEERNYILAKFISEYGDKINAQTINLAQERIMSLKEKYNGCLKNRPNSLDENDTDDNDTDDNDTDDNDTDENDTYENDTYENDIDENDTGENDTDDIDTYEYDTDEYYTDENDTGENDTDENDTDDIDTYDYDTDENNTYENDTDTQVLFTKLNDVKEHTQDYDFKILSRQFSFHPILFKESIIKSFLKIKNSLGLDREQHDFQYLIFQSLYPYDDDQLELVCDIIEKTREHKQEYFCCNLHIANNIQRLYYYNYKFTIGNNNFLQVFYSQLKDIDNNSNLECMKTVLLNTLNPKIRFNANI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVP4</accession>
    <name>A0A8J4UVP4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">FZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_001897</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor Fz/Smo family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000049">
        <property type="protein sequence ID" value="KAF2076771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVP4"/>
    <dbReference type="OrthoDB" id="20427at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050949">
        <property type="entry name" value="GPCR_Fz/Smo-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31787:SF3">
        <property type="entry name" value="FRIZZLED AND SMOOTHENED-LIKE PROTEIN H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31787">
        <property type="entry name" value="G-PROTEIN-COUPLED RECEPTOR GPCR FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63501">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="267"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="FZ" evidence="11">
        <location>
            <begin position="29"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2076771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="343" mass="38697" checksum="95890CAAB17999FD" modified="2022-05-25" version="1" fragment="single">MDNNYSTTTICIRYFVLLVLFLNFSYAWDEKGRCETLSSTSVCNSILGTNQTNIYLKSDQNQTAIEASFSYFFGITSAAGPDCAPYLQKLLCSYNYPQCVIVNSTTPTIYLPSLLCQGDCKITEQICSAFVALFPPEYLCSNKSSDGLPSYPDSSTIYDLQAFGGPSNETIQCSHYSTQAQPATLQCPHPLLNVSYQQQKDQPAYFSLYEIDPESSCVVPCPMQISTKREVDAMYISKVVLYFLSFTGSVILFITFGLLTKKYSKKYEIILSFTFSTVIVDISFFLELSKPDLQFVCGDEPGRYITQTDVRCGFSGFLFHWGTMATLFWWAFLCYDFYLTSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVU6</accession>
    <name>A0A8J4UVU6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_009561</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000742">
        <property type="protein sequence ID" value="KAF2069118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVU6"/>
    <dbReference type="OrthoDB" id="1703270at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11380">
        <property type="entry name" value="Ribosomal_S8e_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.168.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.168.20">
        <property type="entry name" value="Ribosomal protein S8e, subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001047">
        <property type="entry name" value="Ribosomal_eS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042563">
        <property type="entry name" value="Ribosomal_protein_eS8_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022309">
        <property type="entry name" value="Ribosomal_Se8/biogenesis_NSA2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00307">
        <property type="entry name" value="eS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10394">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01201">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="8"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2069118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="211" mass="23859" checksum="F0B30D106CB62FA8" modified="2022-05-25" version="1">MGISRDSMHKHRHTGAARKPNYKKRAYELGRQAAKTKLAQQGEDSRVRPIRVRGGHYKFRALRLDTANVSWGSEKTTRKCRIINVVYNATSNDLVRTNTLVKGSIIQVDATPFKQWYETHYGVSIGKKKAVKTETAETPVAKKSASLLLKLAKRNKTRTLESSLESQMAEGRFYVHISSRPGQIGRVNGTVLEGKELDFYLKKIQKKKSAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UW15</accession>
    <name>A0A8J4UW15_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zinc finger PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_001638</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000040">
        <property type="protein sequence ID" value="KAF2077071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UW15"/>
    <dbReference type="OrthoDB" id="21264at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger PHD-type" evidence="5">
        <location>
            <begin position="651"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="209"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="463"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="531"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="611"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="751"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="837"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="946"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="463"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="538"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="611"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="756"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="782"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="840"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="956"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="985"/>
            <end position="997"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2077071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="997" mass="110291" checksum="FF6DE6131604B95E" modified="2022-05-25" version="1">MDNILFNPFEKDSSSILLQSASFPNVMVVPNPSGSSDDVMSCFSQTTCNNNHINNNSNSSNSLNIYTNSTPNNVDEFLSNSDNDLIDSNIGSTGSSVSSSVTSSPTSIICNNNSNNNILPNSHPELVFNNQSQFPIQNMPGVVYYPNSISTTTNTTNIYLTNNTNSNNTIVQPTITIPTSTTTIFPQTYFPPNTISPTPTPFLVQNNLVPNNNNNNNNNSQQVQQQQQRPPMITINNNNNDTLPEYERHIDSLIKTLPSNSNSLIDLFLHLSDQQENLYRSLLKDNIPKMASSPDQTKFLYDLYLQLMNCCNQAFNNSCKMLAFQKLIDHLSTTNAIRPNVFIVVYNTPFLLNIFKDYLSKKSFLFKVLENSQNISKEELKNNFILLTSRDNFNSLDLINSNHNREIFLNYDVSIVEFDSFWMNQQFPLIPSIPSIITASGFKTFRLLTLGTVEEWLFNKVNNSNSNNNGNNQHQQQQQHNLSTSTSSTPSSPSSPSLPPLSPNTISADQSKEIMVEMMKFCIVKLSNLKKSASPTPSSTPTPISSPPSMAPSSSPLLFSQFQQKSISLNSSSNSSSSINNNPASILNSKSISFSESPLWKSSTSPIGLSTSFDSNSSSGSNGPPTIHLKTSEDKKKSRSSKKLKSMVNRCCFSCKKEEDSKGRTSMVQCRSCPKIYHRSCAGLSHTPRSWKCTRHTCHQCKKTPNESGGSFFICKGCPSSYCITCLPNGVKILDKAEYTEYKPNTPEATAAYYINPNSNNNNNSTDSDDGLQTSPSPSRPVPIGSVSSSSSSPALLESSFMQNQPPLLQQLHKKQKVQRPTVFILCEVCIKTESSNPPTTTTGVIATSTTNNSSLPTTTTTTTIGNSNNNNNITLMTPPLQNNNIVPNTNTTSQPLPINPNTMVSQHPISYHYQQQPPPQQQYLHQQYHNPNMVYPHTIAYPIMNSNHHHHHHNNMPLNLESPSSSSDSSPSPQTQSLPNTPSFYLTDSPSEFMNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWH6</accession>
    <name>A0A8J4UWH6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cullin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009109</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cullin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000638">
        <property type="protein sequence ID" value="KAF2069568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWH6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cullin N-terminal" evidence="2">
        <location>
            <begin position="75"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="217" mass="25823" checksum="5A3EDAFBF77D58EA" modified="2022-05-25" version="1">MKQQLRPFFQRFRFHLDKFPDEETTNNWRLLKARMILSLTTTTTLQTPTTTTTQNNDDYLQEEMFKYVLLLNDLRYYHILYKKLEKLFNNHINSYILPFLLNNNDDDNDAHFLTKLNSSWQSYKVSMHLFKRILYNIELFTQASQKPITDLGLSLFNDLVLRSPPIKEKIFNSLVLLSMKIQQGDTIDLYSIKSIGEQMILNAVKKNQTIFYMNTTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWY4</accession>
    <name>A0A8J4UWY4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000517">
        <property type="protein sequence ID" value="KAF2070260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWY4"/>
    <dbReference type="OrthoDB" id="23204at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23524:SF1">
        <property type="entry name" value="MRH DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23524">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G04850)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035288708" evidence="1">
        <location>
            <begin position="22"/>
            <end position="1231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1231" mass="138146" checksum="FB48617F4F4A2833" modified="2022-05-25" version="1">MNLLLILFVSLLIYYYKGIVGAVCSHEFNYQNGLQCAETLEVTMDLWIGHSNNDGYSFTITPNTSWSEIDNQDYIYHLAAGANYTLEWKHATDSSCDGAEIFSVPNPHFVISQPKCRYSRGTVTFDNPDLIPFDADCQDEPCEVSIQDQDLDLNYHNSNIDCVFKPAFYELSDKYPNLRVQDSYYYKDSGSIQLYDLSKYSSWNLTFGDNPNQNLPESSAGLWIDLKPTTYSLNLQSDECDLQIIPIEIAPSWPSFYLEFGNNIECPRNSSMKLIIDDADFFAKGCQAYINSYSAGNGQSVYFNEYSPEKIEVKFLWNSEMMLKDMYRPDIMGDIQYSVDSCTGIVTLHYDEALYPDVYAYDGKKLPVTDKKFKLTDNAAYVESPCHFSKVYINIRSSILPAYEITKPQQFCGDSMDVFVANYYEFEELTLGEDDLEHDGHGNFKNIPASFHDTISLNYKANQCYETQSIDIPFRSYEPLEYEEIIEIVKYPACSLPGIVSYTIRDIKRNVYSQKQIQSFYTSSLVSLSTSFDNNCSQIFTTDWFSPDIQKYHNSFPNDLYSITKNATCKYSNDGSILFKLNPGFKVLNILINNIVYNSTTDLNGVNFNGLPYGEYEIIIKTDKCSPFYQTISISTTNLWEFPAVVTPVTGDCSVSNGKITYDTSIFTSTTPVSNFAALNSGYHQIDFALSNGSCSGSANIYVPTATGTVPNAKILAQPSCEYSDDGVVKVTIVNLGQESSPTSVQLKSFNTSTNTFTLKGGNYNFMVYNNSCSWPLNVVLKARDVQFKVNYIMDNNDECNQKTFVSVTPMDKNITINDISTDSEVTHYSNFIFSFNPIMFGKNRQFFINYNDHCLKTVTIQDTQDMFQKISWPSIQAKYVDCGGVSTQNTVADITNKKNMTIYQNDNSIRNMYLPTYSFYRSYFTAIDMPSKCSKEINFNNFNIGPISKSISKPICPGSNDGSILVNLDPKSNYYQLWDSDYSHLPVESSSKLNTYKNISNREYSLVKSLKSNLFCTVLEPINIEVNEPTVSLGLLGLCDASNSDIMRGVTNTLSISTTNVTYNINGVLSTNPVFKNLSPGNYSSTATIYNSVCKRVIKSNTVLVPKSPITLTIDTFTCMKAVVTLSQTDSLFKHTIKDSKDTVVHEIETKESFTYTPTTQGTFSIVVSDKGSCLFKSSFIVTGCGGSSSGNGNGSQSPNTSESETVPASSHKLLPSLFFISLSILYLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXD4</accession>
    <name>A0A8J4UXD4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008350</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000506">
        <property type="protein sequence ID" value="KAF2070333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXD4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="764" mass="88387" checksum="01E0E60E5821D302" modified="2022-05-25" version="1">MIIECYRSVFNNKYLAAKIFDTVHEIQGDRYALRYDDIVDVGWMLKHGHLALAREKIQNSKSNNIHNSNRYLYVSPEDLFSIVANTDTQMFMSLFEQNKYEALKYYELLEAGFAKLVNVDVFKYLFENGYGRDLDDKLLPQSMDIKVLAWLLENRWFRPTPTWLIMNKKDHADNSDDSSDYTLDIQTTNYTKEFVDLVARFTPSPIQHADIQAIVTYMLEESRPCYLIYDALSPLFVENPEIGIELGKGLLNYQQALEEIQAKRDCQKKIETNISSSSISELLVLANTPGYRIERKMGIYMWSTCISQGEHVFLRMWDALSKRIRVTEFSVYLGLTNDINDMPEDELEMDDDLVGISDIIVDTACEVGALKILDFLMAKGLTQPVYFALMDNNQFLDRLMDKTLSQEECNIVLRISGGPDEDGWLIDKSAALSSCCVRGNANNFNSLYPLFKQGLGTKMDYPVRNLYPACIQSGQYHMIKVLQDHSLFLSAHKQTLFLFCKRYPFRYLLQDIDRYIAKQQGYCGAFYNMFLEWAINQNDLVAVKHIFTQHKFDSIPSRALNKLPELQNLAIIDYINRNRSQCFTAAAAGPTNNVFGGLLEPSKLSSLNIPLLEYLINENCTGDVNVDHIIPFFARDSQIEPGHSFHQIVYLIDHRNYVSIDPFIPILVDSPDWSQCFEYIYRNQHRFQTKPSFQSIFDYIVANLTGEDIQESLQFDKLYALVHRYKCVLNDSHYQHLTLFGIVPNSNFLSNDYHQPKKQKKENE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXW8</accession>
    <name>A0A8J4UXW8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 2 profile 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_007636</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor 5 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor Fz/Smo family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000419">
        <property type="protein sequence ID" value="KAF2071038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXW8"/>
    <dbReference type="OrthoDB" id="17520at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000539">
        <property type="entry name" value="Frizzled/Smoothened_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022343">
        <property type="entry name" value="GCR1-cAMP_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022340">
        <property type="entry name" value="GPCR_GCR1_put"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112:SF8">
        <property type="entry name" value="CYCLIC AMP RECEPTOR-LIKE PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 157-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05462">
        <property type="entry name" value="Dicty_CAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02001">
        <property type="entry name" value="GCR1CAMPR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02000">
        <property type="entry name" value="GCR1PLANT"/>
    </dbReference>
    <dbReference type="SMART" id="SM01330">
        <property type="entry name" value="Frizzled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="155"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="12">
        <location>
            <begin position="90"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="35"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2071038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="388" mass="44495" checksum="3623D8A3F81E3E01" modified="2022-05-25" version="1">MKIKRFDRIIVISIIYYILLLNVLYITPTLSRESTLSNKNTTTTSGIGSSSSSVNNDNSSNKNNSMSSSGSIDIDDDDINSFTDEQIESLDSIVYFSSTVGSIGALFIIISFFLFRAARSFATKLIFFLSLSDLMAAIAYLPFGRKSKAMCDLQGMGLVFFLTSSYLWTMCISISLFMVFFTNRYELNHWFKYYHIICWGVPLFSTFISLAFDAYGLTGSWCFIANPLSFFRLFYYIPLIFVFFVNLCVFVAIRIKIQRHNNSMISRVNIIVSFYLIAFSLSQLPTLINSIQNFSNPNHPIYSLFAFQLFLQPLQGFLNCIVYGWNEGFINHYIEFFEKYICRCRKSKELREIEADKTSLLVDYDNSDEEDNANGIDKLIIDDYSTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V030</accession>
    <name>A0A8J4V030_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_010021</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000887">
        <property type="protein sequence ID" value="KAF2068657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V030"/>
    <dbReference type="OrthoDB" id="427769at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="483"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="4">
        <location>
            <begin position="4"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="3">
        <location>
            <begin position="246"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="182"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="523" mass="58008" checksum="E5636C93BA85B308" modified="2022-05-25" version="1" fragment="single">DTWVSYYTTIEVPYNFGIIDKRFGISIYGAADRLMNIQGMSFDQLKNQSYNSIIKVENNAKDLIISNVELQTIDPLSNAFIYGHNFGMDPASITIYFMNQDGVLLQLITGDNAKYFSDTLLLVQFINKYTTQVQVKKKVNAGLITDFYTLVNSTLVVPPVYCTGFPPDKEVVYPQPTPSPTPTTSSTPKPTEKPQTVCSSDCGAPKGYGFCNNGACNCIYPQSGIDCSSTQVSTTITPNVTDPTIKVNTNDASAKFESILSVVALRELDQQSNQVHSFDLVSNNWILLSSKSNDIYKQYEYVYQLPSTNITSIIQIFDKSHNITFGSQIINMQPSTVKFTFNITSYPFSSGLNSLQLVLMASFKSQESLGSTCSVTDFIEDQGTAEYLRIQIQDRSLYGRFIKYGLVEGRETAITNTLLQDYKQSIRKPDETQAYIGLNIPYYKNNVLLDPDFSLLIDTKSASDRDNSICTQAPKKGLNAAQIAGIAIGGAAFLVIVALVSIFVLRKKTNSTILIKLQKTLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V044</accession>
    <name>A0A8J4V044_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009956</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000865">
        <property type="protein sequence ID" value="KAF2068717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V044"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34078">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34078:SF9">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="14474" checksum="6631CC89E4470CBF" modified="2022-05-25" version="1">MSEHQPYQDQQYYQQQPPPQLSQEQPQQYYQQQQPPPPPPPQVKVYDEESGHLHHHRDEDDVPTDIGAIIFFVTGFCFPLLWLCGLCYISSRNPTSRIFAIISLVLFALAVICSLAIVVPVVVVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0A1</accession>
    <name>A0A8J4V0A1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009723</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000788">
        <property type="protein sequence ID" value="KAF2068957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0A1"/>
    <dbReference type="OrthoDB" id="10662944at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="361" mass="41146" checksum="1ED1D5746F97E734" modified="2022-05-25" version="1">MDYNQFTNLTHALGFNKGFGGGHNSMELCLPNSVLPIKQLQHQQQLHSTSLFPFVETVGHQQYSNFNIESTPIHGASSNSDISNYLNNDNNRIVNYLGLGNLDISNDNFYHSLFFFGILPLSAFIALTSIGPHILRSLSSTKDTFSKWEKEMPSFIPEFFQRVFKGFLCIYFSFSPIYNMINRNEVNLCYILLSIIFFTLADTLILYLYKSKDKSMYLHHMVSLFTLYAPIFFGKVCIPYLSLAIQSEILCCFSLVIGLLKLFNLTNSFPYFMASSAGLFCMLIMRLPKHCWIVYDLLVNQPTSASAITCAIGVCLIIPFDLLYTKIFLKMTLSSYKVLNQMKDKRSNDSLVLKNESIKAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0C2</accession>
    <name>A0A8J4V0C2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BEACH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_003246</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000099">
        <property type="protein sequence ID" value="KAF2075465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="26681at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06071">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1540.10">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036372">
        <property type="entry name" value="BEACH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46866">
        <property type="entry name" value="GH12955P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46866:SF1">
        <property type="entry name" value="GH12955P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02138">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01026">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81837">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BEACH" evidence="4">
        <location>
            <begin position="444"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="2">
        <location>
            <begin position="1704"/>
            <end position="1745"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="84"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="706"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="818"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="989"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="103"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="339"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="720"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="818"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="856"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="907"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="930"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="945"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="994"/>
            <end position="1011"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2075465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="2016" mass="227030" checksum="7D0322A401118498" modified="2022-05-25" version="1">MGQHLSKEDVKLEIEQYLRVCFLSMDQEDRQGSHIQSSFNLSTASAQKESVEAYLHYLSTSKETKPSIDDFIKSFSASIFLKREEEQDDQQQQPDTTSTADVNIKDEDHKDKDKDKDEKDKSSAEDTSDTPDKKEEQEEEEDPSERKKKKNIRYKKGSFEASCFDGDDISIQILKTHIGNSSTSDLNSSSNSTLPTAPAIQLPKLFERVLSQYHQQQFKPQPNEYLNSWEYFDELNCAPIFYDLNPLYNMLHSDQYCIQEKSLCIPSSTTSGDYVITQEFIKGVLKALSTYISPLPNSIHFNDTHIPASIDYTQNNVLRFHPNVLPILEVLKRNSKDTNSNSSNSNSNTEQQHNNTDDDDTFYIIYKKYQYSLDGLIRYSNQYLQKNLKTTNFILYQLVQLLSFLHDKEVVHGNLTPASIQLDNRMWVGVHGFTFPSSPLYHTPALSQSSLQDCAVQRWITGDLSNFDYLMILNKLAHRNIGDHMNHPVMPWIIDFTRNPLLPTTTTTTGTTGNNSTATDGWRDLSRTKYRLNKGDEQLDFQFHNVPEGTENPHHISDVLSELTYYSYLARRTPVPLLRRFVRTNYEPNEYPNSMERLYRWTPDECIPEFFTDASIFTSIHADMPDLLIPEWAASKEEFIQIHMKALESDQVSKQLHCWIDLTFGYLLSGEDAIKAKNMALMDTSVPRNSGIVQLFTKPHPKKILKSPHPMSASAAPLGGSNTSTPSSSLDPNSLQSNANTIALSNSSDHDFIIKFNDFGVDYYSNPISTNRSDSFGSNSSFSAANTNMPLSSIYHTPNSTTANPLSYSNPISSSPLNNNSSNVLNNININTNSGSNSTGTSNNSNTPYQPRVNPTISSNQTNANSNTSNSSGNTNTNSMKQRSSSSKKDDKPFSKYLPSIFQPTRSNSSGSSASAPIDQNINSLKDDSFSTSPSSVPMESMSSGGNQSANTPLSPPYGSSTKHQQPLIHSKSEESMIKKYGNGLFSSMGITKPSSSSPNVPTTNNNVGNNYDRHHHNEPTSPISPPSPISQSDNTMGKPVTSPHLMTAINNSPTTTADLPITQIMEPLPTDLEFGLLEYDLISGNQNHQPRSAASSYQHTGAPDAWDYSYVMAQNSSIFIDKLLNHENNSLFNQNFEKLYAIYQPLEYKYSSIEYPDILCWLSSKQAQKLDIMKSNDMFALGCIISELYQGFPLFTSQSLEQHFNSNRGSSGNNSNTYVPPDYVKNKPIQLYPITYNLPNPIREVVCRLIDPNPEDRITPKELLSSPIFPNYFKQMYHFLCHYHSLYTAEEKLVFTLANIGAVTSLPNEAMDLILPYILELFNHPNTMVSALIDLLDPLSQRLGPLLSQTYLLPCLINLYQRHEDHLLQCHLIQIPMIDMIVSRFGRDIYIHHILPFFLDSLKTNPRENPNHEMLTTGLIKISKILGIPLTIRYIMYPLLVALTKPHLQHLNEPLIAIASSLGESVIIKFYFPAIFILIQKHSSKATRSESIPCTLLSLLQELILLVKPGLVLRYLLKESTQLASLLLNPSNPALLLPLAETLLRISGRIGVNNTKDYILKYLQQFFSNYSDLYGYSGHNSYSLIDTSEASKQLRAIYSPEMTYFLYYKLARIIGFEIMRSEISDNSLIEHIMMIYIKENHIKSETPPSTIPPYVSDEGLYDDFEETLDHKISLTYKLDDYQDYDDLINDQSFSFQGNIISCYKEHNASIKSIAMSPSESIFASGSKDNLVKIWSLESTRSLATYNQHMHTVHSVKFISSLVASCDITSIQVWDPESKVKVNLFYEPNGSYTCFEPLTNKYIVASSSESTLNFYDLSLGIQTHEWALAYQTGSSIRCLSTSNDHHYTSSNASHSSYSNTTTIPTWVAAGSSSGMISILDTRTGTILEQWKTHDGPTIKLIGQGTRYLISCSEKSVIQWDLSNSPPSIKKMWKGFKDAITSMSLCQNDLIVSTGNKITSISLLDDTYSNSSNYNTFRVNEGIRLNVQKQTNIQSLSFFPLHHLLLAGTEDGHIYIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0N2</accession>
    <name>A0A8J4V0N2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF218 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004100</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000139">
        <property type="protein sequence ID" value="KAF2074593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0N2"/>
    <dbReference type="OrthoDB" id="17725at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06259">
        <property type="entry name" value="YdcF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051599">
        <property type="entry name" value="Cell_Envelope_Assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003848">
        <property type="entry name" value="DUF218"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30336:SF20">
        <property type="entry name" value="DUF218 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30336">
        <property type="entry name" value="INNER MEMBRANE PROTEIN, PROBABLE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02698">
        <property type="entry name" value="DUF218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="DUF218" evidence="2">
        <location>
            <begin position="86"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="518" mass="59197" checksum="87C046A351B92480" modified="2022-05-25" version="1">MHNFTNNHNNSNSLIQLVYKYKVRVLVLVVLSIYLLSLLSFKSNETVVVNKPTTTGGQGTKKSVSFQTTTSKTVANDVPLESHNVAITVLGFTLHSDGMPTKILNDRVLLAATHYRELKAGGANPLIFLAGKGKGSDSENVESEYKSEADAMKSIAKNNGIEDKDIIIDQTSTNTAENALNTLKYLESKKIDKLIIATSDFHVLRAQYIFQTIFPSYFELEFIQSTTPPTSLKSLSNKEKALWESSQKDLEKLDLLKIPGEKAFGYHPFRNVPRWKMIEREIENDINRNFRTIPAGATMVDYGSNQGYFSVHLAKKVPQALMVSLEGEAMKEYDDAAVEHEKKMVEFNVTNNFLCRTHVKTPMFTQLAKLKHVYEYQLCLSVFHWFGMKTKEHFEEVLFQHLVNARTTFIELPEAKKYLGNEGQHAWRESNIWFDGRTEVEVLADIKAKYNMKMSWKILGALKHDNKTIRKVIRVDVRSDFDSEDQSDHRRTLNLETLKSTYECGKLMYGKKIKNQEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0U0</accession>
    <name>A0A8J4V0U0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003859</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000127">
        <property type="protein sequence ID" value="KAF2074833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0U0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035168443" evidence="2">
        <location>
            <begin position="21"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="84" mass="8456" checksum="9819311B1E6285D6" modified="2022-05-25" version="1">MKFFALIAIILALLAVTTLAIDGQIPFTNSPHTSATSGADDSGSGTGTGGVDDGCHSGRADPENFCSGQAFFPSTTGNGNSKDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1C4</accession>
    <name>A0A8J4V1C4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001773</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000044">
        <property type="protein sequence ID" value="KAF2076935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1C4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32142:SF55">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32142">
        <property type="entry name" value="B BOX-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140860">
        <property type="entry name" value="Pseudo ankyrin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="928" mass="108251" checksum="2681076CD54A7814" modified="2022-05-25" version="1">MNSNNQHKQQLFLSIWGNKSIRREVLRAIKIINRERRDQLIHLVNSGTYTYYGGTGINPQSLLSTHKRAVTVNPSLYIQHYDNIHSVSWMLQHNHFGLLKEKLQQGQYLSFDYMSGYIITSKLGTPERIDLFRLLYSKYHGKDNFDITVFQSAMTNQKVEIISVLLEYNQLRELFGNNNNPNHRALDITLHTKDRQILDLVLQNGLGKNPMDISPSFIIKNCIGEFHDPSYDFLKYVLSLSESNTANNNGDLYRLPFKYSDLRIPNEKKQFETVTIADLFETIDFDLMVMLVSKKWIYFSTSYFHNCVSNSLAKRYSDCSCADMYNYMKKILYLTIYNLTLKKSDIIHFTNMSNQLERLYGSGSSENQLSVLVHYYINNFILYPNTPLPPYLIVQELFRYSMFIENTDELLKYNSKIGGNYGYFDQDFIISQLFDHSLCNASIYYLKSMSTLLKEGVPTKKISNISHDFESFKNCKLDFDKQKQYLEFLVRMAINFPTWDFQLNNIFLSLNYLAIDKQEKSQLILFLYDLFVQFNIYQHTTYSMATSETIVVGISKGWLEFIEFILDPERIDNFELPVDMYNLAISHHNQEMVELFLSKSIPISNNALDTAVEMGNLSIIKLLNNEGKTPIKALSYLKAADNLDLDVLEYLIDNRTERVPDLIAKCVHLDSSVLDLLSKKSFFFSTGRFGDPQIIESHRNAVGGKIETSFLADFDNLRSKQFYQIACHDVELDLSGESGEREIVHPNFIKFVEHISKIGDLYIINCIFKSAKPYYDAHPELKREHMKKILYRSLLRSNFHVIKYLREQGLIEPNLIDFVTLAGSHFKSMVLRRQDFLEYLIDMFEQDFKKTLTKMEIFSILKMSYSSGSHAIFVFLLKRFPQYDLLHDLKKETILCPNPNIDSFINNYHNHSPSSSLSKRKNIFKINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1M3</accession>
    <name>A0A8J4V1M3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cytochrome P450 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_001439</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000034">
        <property type="protein sequence ID" value="KAF2077250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1M3"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20617">
        <property type="entry name" value="CYP1_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000068">
        <property type="entry name" value="Probable cytochrome P450 508A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF417">
        <property type="entry name" value="CYTOCHROME P450 508B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12 13" id="KW-0349">Heme</keyword>
    <keyword evidence="9 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="433"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2077250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="487" mass="57003" checksum="69234ADD718CB8D3" modified="2022-05-25" version="1">MFILSTIVILFIFYIYINYFAKFKSYGKKSLKGPMPIPLLGNLHQFDWKGVRPNNPWIYFHSIYGKAYRIWMGDTYTVVINDIDLIKTLLIKNSEITLGHPDTPTFNYYSDGYVDLILSKSNDWKEKRDLISRSITKTKIKHIYKLLDHQIGNLLKCMEYHRVNEIPFTPRKNIQRYAMNTMLRIIMDKEIPYDHQGVGDGQMKDLLHELDILFLDMGAGKIGDYVDILKPFLFYYYKLFNTCLPRIKQIIKVEFDKHLSTFDPEQHISTPRDLFDIMINEYQGDKEKIPSILSMGFDVIFAGTDTTSGTIEWCLMYLVNYPEYQEKCYNELISVHGNDQITLDHRQSTIFLNAFIKESMRSRPIGPFGVPRSCSQDIELEHIFIPKGAQILINYCGISRNPQYFDNPYEFNPFRFINSNQDFTMFGWGKRNCPGQLLASDKIYLAIANILLNYKLKSVDGKPIDDTDYFGMTLHPSLFDIKLEKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1T5</accession>
    <name>A0A8J4V1T5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA mismatch repair proteins mutS family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_007904</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000447">
        <property type="protein sequence ID" value="KAF2070772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1T5"/>
    <dbReference type="OrthoDB" id="276261at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032301">
        <property type="term" value="C:MutSalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000870">
        <property type="entry name" value="MutS protein homolog 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011184">
        <property type="entry name" value="DNA_mismatch_repair_Msh2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF146">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEINS MUTS FAMILY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005813">
        <property type="entry name" value="MSH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="5">
        <location>
            <begin position="775"/>
            <end position="791"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2070772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="917" mass="105386" checksum="920779185CEBA5CE" modified="2022-05-25" version="1">MNDFTEEFDIYNYLDDSWLDTKNNKNNSINKNRINSQKENRELDNDELEFIELEDILKSKPDNQSLKEKNEIEDYNLDEFVYSPLTIENNNKKEIVNNNNNIISTPGINNFKFKNPNTPLPKKITNLKTPKSLDSIIKSPTKKNVLNTEIKDNTQTIDKVLKKTPISSTINNNNMFNIIALTENKSREIGYSCYNQVTGEIEIGQFCDSQTYVHLYTKLYIHTPLICILPPIEQDSQMVKMIEQKFPRTKFQPFHRKQFNENVGELMIKQYSVPEKYISIEKISFKKYLAYGSFHALVKYLEGFHDVNFVNQCVNISFSGCERTMIIDGETIKNLELIRNNYDGSRKYTLLDSINKTKTAQGTRLLISNIVQPPNDLVTIKLRQDAIKELLSDETIFNSLVPVLSKIGDIDKILISFSQTKKKITSIKKAESLIKQFIEIKKIFEILPKIVDLLKSKENALLKSINRNLSKPIINQLLKENHRFINEKTPTLKVISSTKIVYSIKDGIEPLLDVCKKTFFETNDDINALSKHYQEVYKLSSLKIQYSTTRGHYFSLPCKNKSLFHLPDIFIKSTFANQKCTFTSEDLISLSRRNKEVFEEIILLSVQSIEKLVYTYRLSISDLFNISESISLLDYLMSLANYVVLSSDSVVCPEIYSSGPIGIQKGYHPVRLTQSMINRKTTFIPNDTLINQTSNFQLVHGPNMSGKSTYIQQVSLLTIMAHIGSFIPAEFAIIPIVDRLISRIGTSDNMQSNASTFMTEMKEISYILEFATEHSLVIVDELGRGTANLDGSSIAWSISERMAEIGCYTLFVTHYQELLKLAILYPNIRIYHFMVSRDENSLNYKYLLNEGVSDITRYGIDIAELAEIDQRVIDAARIIRNSLDDINNNDNSNNRDEIDYHPSRIEYQISITSSKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1W4</accession>
    <name>A0A8J4V1W4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GOST seven transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_001135</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000025">
        <property type="protein sequence ID" value="KAF2077595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1W4"/>
    <dbReference type="OrthoDB" id="19932at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053937">
        <property type="entry name" value="GOST_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009637">
        <property type="entry name" value="GPR107/GPR108-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229:SF1">
        <property type="entry name" value="GH17801P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229">
        <property type="entry name" value="LUNG SEVEN TRANSMEMBRANE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06814">
        <property type="entry name" value="GOST_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035295950" description="GOST seven transmembrane domain-containing protein" evidence="7">
        <location>
            <begin position="22"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="282"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="328"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="359"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="405"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="438"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="GOST seven transmembrane" evidence="8">
        <location>
            <begin position="221"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2077595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="550" mass="61633" checksum="947B558F4E74E63C" modified="2022-05-25" version="1">MKRYLLILLILAITTISFVQCSRVAVDYSTNILQSQVQGAFIYSPQSSVPHSKPGQGFSYFNATLALSLLYPDQGINFETQQAAIDILIFRDGAPLGVTYDGVYSLCCSQTLFSLNICPMVGAVIVPYATKNASISGTNFKDFYSETADVASHHITVHSQLNVVDNVQVPVSFEITDTGIYYAFLLSCNQQLNVENNVYLSAKGDILFMNPFGYISGELFPLIYFDLILALAYIGLGILWVVLFLRYRSTALRVQYWISLVIVLGFLESITDYADLSAQNKTGYFSYAGLFFTAFLGTLKRGFARSLVLIVSMGYGTVVPTLPKNVTLKIVAITLAYIVFRGVYAYLSGLPRQSQSQAASGVLLQLPVAILDALFYFWTLMSLSEILKRLSERSQNVKIQMFRKFIWVLIYSISVTCVIIIIQLFCNWVKSTEFNIKYSWVLTAFWDTAYFAVLGAICWIWKPNENNQQFAYSELDTEDSVVMESTGGGVVTQRNSSQYDADGNKIDSPNESTNSFTIVESLDDEMDHTINGDDDEFDKKDLADRYFKSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3S6</accession>
    <name>A0A8J4V3S6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Armadillo repeat-containing protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_001739</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000043">
        <property type="protein sequence ID" value="KAF2076963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3S6"/>
    <dbReference type="OrthoDB" id="5559898at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034657">
        <property type="term" value="C:GID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001256">
        <property type="entry name" value="Armadillo repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038739">
        <property type="entry name" value="ARMC8/Vid28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000357">
        <property type="entry name" value="HEAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15651">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15651:SF7">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02985">
        <property type="entry name" value="HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="174"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="174"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2076963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="696" mass="78117" checksum="2762F2EA2C36A80B" modified="2022-05-25" version="1">MDEKAKQSLVILKQRDQYLNEPDIPIQAAKYIKNSIIGNKNKKRLFINLDAIPVFVDILSRDKNDVLLIQCASIIGSFACRIDEGVQSIFENNGVTPLINLLSHSNPKLVESSARSLKILSLYHHPPSSFIYEESGIKLLVSLFRHPLESVKEVAATILARGCESLSESTLIKNSTNGISSNNNNSNNNSMMIDYISTNNDNNNNNNNNNNNNNNNNTGGPNTSLLQYQNIVHVFGGLQAIVDILLSPKTKIHDTCLYAIGWLTRDNVSLSTFILEKDKRVIDTIIQLFKDDNPKTKLLAATCICNFHQSKVPSTWSKNLIKNILPIIIRLMSEEESVKDEIPIVIARLINDSEDLQRVASEAEAIPRLGSFLKDSSSSEKLKENTLSAIAVLCSLREDSRRQVVDAKIIPHIINCLNSNNYAIRAAACRCTKSLSRSIKQLRTTLFDCTIAIPLLKLLDDASLEVQISSSATLCNLTLDFSPMKQTILDNGGVKKLVSLTQCQDFQLRLNCVWALKNLLFMADASLKEQVIKELTYPRLLALIKDTEHPIAEQALSVLRNLAYKDTEELITDEKFDNQLIPILESKLTNDSPAEIIKQTLFVICNIVGKEKQRSSVMKSTIMHKLLNFTEHKNPEIRVAVVWCISNLLSPEDEGLPSRISKLKELGYHKKLEALREDQNIEVKERVKTALNIFKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3Z6</accession>
    <name>A0A8J4V3Z6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidase M20 dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_005622</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M20A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000229">
        <property type="protein sequence ID" value="KAF2073067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3Z6"/>
    <dbReference type="OrthoDB" id="3064516at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990845">
        <property type="term" value="P:adaptive thermogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043604">
        <property type="term" value="P:amide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043605">
        <property type="term" value="P:amide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05674">
        <property type="entry name" value="M20_yscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.900:FF:000003">
        <property type="entry name" value="N-fatty-acyl-amino acid synthase/hydrolase PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000027">
        <property type="entry name" value="N-fatty-acyl-amino acid synthase/hydrolase PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017141">
        <property type="entry name" value="Pept_M20_carboxypep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047177">
        <property type="entry name" value="Pept_M20A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962:SF2">
        <property type="entry name" value="CARBOXYPEPTIDASE S-LIKE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962">
        <property type="entry name" value="N-FATTY-ACYL-AMINO ACID SYNTHASE/HYDROLASE PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037217">
        <property type="entry name" value="Carboxypeptidase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="10">
        <location>
            <begin position="290"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="509"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR037217-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR037217-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2073067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="538" mass="60198" checksum="09575FC730F6C083" modified="2022-05-25" version="1">MISSNLSHRGGGNSNSSRSNDSNDIYNSKKEKLKKNGNSNGNSIFSIIKKLLKYGSLLLLAVMIFNTIMFTSKQPKVQTLDPDHIDSFASLTENELANRLSKAIQFKTISFGENKEFDHYEQEFLKFHSFLKQTFPKVYSNLEFNIINGYSLLYHWKGSDSSLNPVVLSGHIDVVPISNLDLWTHQPFDGTIADGYIWGRGAMDDKQSVMAILEAAEELLGQGFKPSRSLYFAFGHDEELGGAQGAQFIAQHLREKGVKKIEYLLDEGLPILLPPVFPGISRPIASIGVSEKGAMNVELSIDIVGGHSSMPGRETAIGLLARAVARLEQNPVKHSTKETSLLFDFVGREASLPFRFLFSNLWFFEPIISRVLAGKPSLDSLQRTTTAITMFNAGNKANVLPHSASALVNFRILPSDGIDNVVKHVIDTINDPRIKVNITSRTEPAPVSPVTAPSFTLLQSTLLQEFPDVIVAPTIMVANTDTRWYWEFTSNIYRFCPMLIQNSDLTRFHGIDERISTKNYHQLVDFYYHLIRNGDKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3Z9</accession>
    <name>A0A8J4V3Z9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_008372</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000510">
        <property type="protein sequence ID" value="KAF2070304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3Z9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31318">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035223448" description="C2H2-type domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="478"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="4">
        <location>
            <begin position="498"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="440"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="522"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="447"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="531"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2070304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="563" mass="61283" checksum="979423831AB8DB24" modified="2022-05-25" version="1">MRYLYTLSFFFILLNIIYGGELSLFVNPSAANNNKQCGSSIQSACDSIVSALASYSSQNSANYSTTLTLQLMDGNYTAPKNSFLKNIYINTININSFSNNSANVIFNGFNATYPFLNYLQTSTNIPLNLLVNNVTFINSTHILNSTSTLSVTFDGCVFRDVLGRTSIIYLSNSNVTSLPSVNFINSQFFNVSIQGKQIVVANKYLFVLSNMTMINCVGLYGFDLLGNGIIANVTIDSTRTFGSPLNLDIINSVTIRDSVFNNNTGSTGAIAFEDETSFSALIQNSNFTKNKSLYSGAIYLGGNRGSYRIVGSNFLSNQCVNNGGALSSIGNTVSVQTSTFVNNTALKGGALYATLSAYVYLNSVSLSNNSADEGGAVYTLNSFVSLNSAVLTNNSATYGNDVECTSSTLSITPSSSMIKSNYWCPYSDCTFRDQSNFQCPTDNPPVDSSSSHSKNNTHNSSSTNDDKDKKKGLDRGDIIAIAVCVSVVGVIIILILMCLGCRKHFTHSNNSSGHVRVNAHYKSHHESSPLVHHHHDSHHHNHHNHHHHNHGHHHGHHHHHGNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V545</accession>
    <name>A0A8J4V545_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0538 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000366">
        <property type="protein sequence ID" value="KAF2071519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V545"/>
    <dbReference type="OrthoDB" id="937at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018794">
        <property type="entry name" value="UPF0538"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18444">
        <property type="entry name" value="UPF0538 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18444:SF9">
        <property type="entry name" value="UPF0538 PROTEIN C2ORF76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10209">
        <property type="entry name" value="DUF2340"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="125" mass="14802" checksum="2E600910AF5EE956" modified="2022-05-25" version="1">MENENKILIVRVIKSMEYRTIKNLLLKDIDLNTNVAEFKQIVREKIQTTSGFLPYRNKEYDSMKIYFIPHGQKSSNLTINLDQDEGLVLDDKKTLAECGIVYETEISFYRNEEYIKFKANPEVKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5S6</accession>
    <name>A0A8J4V5S6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_006578</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000310">
        <property type="protein sequence ID" value="KAF2072104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5S6"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051938">
        <property type="entry name" value="Apopto_cytoskel_mod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44145">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY A MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44145:SF3">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY A MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="6">
        <location>
            <begin position="26"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2072104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="379" mass="43537" checksum="AD5FDCF546D502B1" modified="2022-05-25" version="1">MNPLHAPPRHEEITEIMSATFNGEANLYLIMEIADEEVSIEQIKKQYRDLARRYHPDKNPNATEQDRERMSMIVPAYKVLSNERLRYLYDTYKFSAKESRRTGANKRMVLYELFTLGLSVASFPFHNLDTICKARPRVKGGLSDSPWRVFRERGLYSLYRGTVFTTLTDFVHSLIEKRLQGTYLAPIGIGKVLGYPATLISNLIVMNPSLTLGALVPALLRGAKDKEFDFKNLWYGYSIYALTVGVDLGLNYIFNKIREKSFEKYIKNPQSKLWRYISYVSSYQLFTSGIPTLLLCPFDVVLYQYQYRLLGVVQSGATVASLGALSLKNTVLSIYQTQGLSKFFSGLVPSLVYRFIADYAFEEGERLDDEYDENPEEYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5S8</accession>
    <name>A0A8J4V5S8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PB1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_000255</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000005">
        <property type="protein sequence ID" value="KAF2078388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5S8"/>
    <dbReference type="OrthoDB" id="9450131at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05992">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051864">
        <property type="entry name" value="NCF2_NOXA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15175">
        <property type="entry name" value="NEUTROPHIL CYTOSOLIC FACTOR 2, NEUTROPHIL NADPH OXIDASE FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15175:SF0">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING PROTEIN C23A1.17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="35"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="PB1" evidence="5">
        <location>
            <begin position="304"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="181"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="388"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="190"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="222"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="241"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="280"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="396"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="433"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2078388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="457" mass="49617" checksum="1358862D02EBFBFA" modified="2022-05-25" version="1">MLKATLRKWNQSIDSYGRGQTNEAIGLLTSVDPTAKINYNIGVMYCKNANYAASIDFFTRSIDLDKWLAASYFMRGVANHLSGNLNHAIVDYDESIQRLRGHEYIDYRQLGLDTKLLLCEILFNKAIALGRAGGSVAAQALQCMVISEDPSFRQECKRLQDTGGSATFNIRMIPINLLFRPPKVSDKDGPSSSASSASSSSNSIPIPKSSGVSTASSGGSLPSPPVKKPSPPLPTPPKPPSVLSKSFSPTTTTTTTSYSSLSSSTGTRPAPSPSFASKNPPKPPPLPKKPLPSRPISMIVQEVSITIKVTYKDKRLIQITNPCNISTLRQKVDIKFGINSRDLYFKYPYSGDNEYYPIKSQKDLDLALSIPLTDLVISDYDDTDYNSNSSYSSSYEQPSHATTTTTSGISLEKSYNTIPTRPTQSRPAPPIPTASNTGTIGRSNSRFAPPIPARNPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5Y3</accession>
    <name>A0A8J4V5Y3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003416</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000106">
        <property type="protein sequence ID" value="KAF2075287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5Y3"/>
    <dbReference type="OrthoDB" id="5353095at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20556">
        <property type="entry name" value="CYCLIN_CABLES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012388">
        <property type="entry name" value="CABLES1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22896">
        <property type="entry name" value="CDK5 AND ABL1 ENZYME SUBSTRATE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22896:SF0">
        <property type="entry name" value="CYCLIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0195">Cyclin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="3">
        <location>
            <begin position="644"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="13"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="288"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="495"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="42"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="248"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="292"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="311"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="390"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="514"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="765" mass="84853" checksum="0D6750438F9E8C8E" modified="2022-05-25" version="1">MSKAAFNFLSNISLTSSSSGTNSGGSSGSGSSSSKKEEKKPPSSSSSSSSKTTTTTTTTTSSSSSAKSSTSSSSSSSKLKTSSQNTTFNHQHSHSWIFNLPYQSFKSLREIKLNSRVKLKIKLQQVSLKKGDRFYFSVSSPTTTTTSESKGDRIVCSSIVIIDPTEVQDENTTEVVVQFEKVFNDPIPRTVFASYPTLHDLAISKSTFTKISDSQLQLFSDVLLNYSSKVAKKEAVSFLSGISLGDINSSSKSSNKNESPSMSSNITTSRNDLGALLDNNVIDNNEKRDILTTSSSTSTANTRRDTDSDLIKTSNNELVNRTKSKDNIITTTAAVNSTPKEGFQPPSFNRPPLPPSLLTEINDLLNKNNHQHQPPPQQPNNNNINTSGGSNLLVASNTNSNSVTTSAESKSDFDDAKSHLTVLKEELYYDRLNNSNNSVTAGGDVYSSQQLVKKQKRSTIEEYNQQYNLAFQQKIFLVTPSGHPFYVFSMIRGESRKPKKGGKKGEKDPDMLGSRSNSNVLHSSGMNIDGEILHHHKEHKKNRSDEVSYAHLINSTHQQLQNLVGDEVDMNGIPLKYNPGFLDNSDLKTGKHRTVIYLPSYKISIFPFIKKGAIKEELNEQFRQKHQWITQPSMTLSKIRKMKRKLKKISILSGFEVSTLALSYVLLEKLIIKNMISKPNFRLYGAACLLLAAKFNDTKAFDRELSDFFEAVEKKFSISKKDLIHAEFLVFTHVSFGLFVDFHEVLPHLNRLKSESIDDDKGFIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V662</accession>
    <name>A0A8J4V662_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003204</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000097">
        <property type="protein sequence ID" value="KAF2075512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V662"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="241" mass="28741" checksum="B54F2423A40269CF" modified="2022-05-25" version="1" fragment="single">MNSATKKNKKKNQETNEVERLTKLFEQQLKEVRDQHNAEIRKKVEDIKMKDEKIKMNEEEIKMKDEEIKMNEEEIKMKDEKIKMKDEKIKMKDEEIKMKDEEIKMKDEEIKMNEEDRKIKDEIVNKIELLNETKKIKNLFVKFPTISKTNITLKSSSTHQDCECLEDKKFQLLPNFEELALKNGEKESNLIAILRSKFYRPIAENEQALYKFVEAVFVDALQILDYESEISIEIEKSLDGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6A0</accession>
    <name>A0A8J4V6A0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_006073</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000264">
        <property type="protein sequence ID" value="KAF2072614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6A0"/>
    <dbReference type="OrthoDB" id="1068471at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000375">
        <property type="term" value="P:RNA splicing, via transesterification reactions"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000229">
        <property type="entry name" value="Small nuclear ribonucleoprotein U5 subunit 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052234">
        <property type="entry name" value="U5_snRNP_Component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44006">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 40 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44006:SF1">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 40 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="68"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="112"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="196"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="239"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="300"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="331"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2072614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="366" mass="40590" checksum="F8ED6496CC51DF0A" modified="2022-05-25" version="1">MDNSKKKRKQNENNNGALIVTDNNSNKGTPKKSKKNNDLAIVTSQGGGKEIILGTERTSKLSSPIMHLTGHRGEVYTVKFDATGNLLASGGFDKEIFLWNVYGEECKNYSVLRGHKGAVLELHWSTDSLEMYSASTDKSIGVWDVENSKLIKRIREHKSFINSCCPARRGPPLIASASDDNTSRVFDTRMKASTHSFKHQFPVTSVCWTDASDAVITGGIDNVIRVWDLRNSDEPSMVLNRHHDTITSLSVSKDGSYLLSNSMDNSVSMWDLRPYAPANRCLKTFIGAQHSFEKNLIKCSWSPDGNRVTAGSADRLVYVWDTNTTNLLYRLPGHSGTVNEVVFHPTEPIIASCSSDKTIYLGEIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7V9</accession>
    <name>A0A8J4V7V9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Sm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_001461</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the snRNP Sm proteins family. SmF/LSm6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000035">
        <property type="protein sequence ID" value="KAF2077207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7V9"/>
    <dbReference type="OrthoDB" id="268799at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005732">
        <property type="term" value="C:sno(s)RNA-containing ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01726">
        <property type="entry name" value="LSm6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000037">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016487">
        <property type="entry name" value="Lsm6/sSmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F SNRNP-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021:SF1">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 13" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="10 13" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 13" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="9 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="8 13" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Sm" evidence="14">
        <location>
            <begin position="14"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR006609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2077207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="87" mass="9754" checksum="81DEC16224DD8449" modified="2022-05-25" version="1">MSTQTAPGASNSNAQFNFFKMCIGRPVVVKLNNGVEYRGILEVVDGNMNIVMEQTEEYLNGQLKTKYGDCFLRGNNVLYISAQPKRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V803</accession>
    <name>A0A8J4V803_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_001393</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000032">
        <property type="protein sequence ID" value="KAF2077327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="26687at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056651">
        <property type="entry name" value="GlfB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36127:SF3">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36127">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24929">
        <property type="entry name" value="GlfB_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="5">
        <location>
            <begin position="533"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="4">
        <location>
            <begin position="738"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="3">
        <location>
            <begin position="896"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="84"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="127"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="236"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1306"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="84"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="248"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="289"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="304"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="364"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="424"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="447"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="461"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1335"/>
            <end position="1346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2077327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1436" mass="160512" checksum="246053A6802F5816" modified="2022-05-25" version="1">MTDINAESFSALNFWKSKLNFPLLAPPPPPPPPPAAEEDFKSNKSEWDEQNVTGAFGFWKQKAIQLQQETERYNARRNSRATVDLTNILRKSTSNSDLSSPDQPKTPTSPHSPLDIIATTTIVKTTTTTTTTSQFENEQHHHHHHSNSKHPEVTVTSESEDEEYSQKTTTIETTNTLIANQLEIKVGGETIVVVDDHLESKKSPRDYRRSRSISCEIIPLVPNATSTTVITESITTTTIVEEDKQSDAESDSSVEDSDEDSDSNDDTESEEYEEQVDTSSIKHHHAANDENSSANNSSGDLSSMVHTPVTSVDDTMKPDQASLDSAGSNAVTGTSSTTANINGPKANQPIQQPGAVTLVHHESQSQSQSTQPQPQVPAVPDANNNNTKTPSSSTPSTPNATASVATNAANRPTNPVKKDHQKSKSIGATLTRTVTKTFIRDSKESNKMPSTGTTPTSTTPKTKEPKVKLSKQEKERIKQEKQAQKKKEKDEKKQQKKDKKPIGKSLSLTDVKKPSSSSSSTSSNFADTKIFKVRLTQLVLANEGSLPAILTSTITVLSNSENVDVKQIFVGAENDSEVKSIRVRSDSGEKIDFSLINDTRIVAGLLILFFAELPQPLFNSKFYNDLIEIHDITNPQVKLNDLRNLISSLSQLRRSLLQILVTFFLSKYINNNPTMGRSEALQVMCMVFGPIFFRDIDPKTKHLNVTVREETLKLIIDNYVFLFEKTDQPDLKYESVDGKLLISEASVDKLIDKATDYYYPYNEKYFSLTFFISHIYFISASDLVDKLSTLFKENHIDTKKKWKKQRRSKKANCISESVRLWIDYCYREMREDKKLSQKILKAFPHLEAHIGSRLTQSRGFGNILRVPKFHSRTRSASFSESFLTTTTGVGGKDQLSAVEIAEQCTLADYDLFTNVRLSDWVRLMHGSVDPSTAPSLSAALKKSTTWCQWAMGEILSAEDKSQRVAIIVLLVDIAIACRDLANFNTAISILNALNNHHIKRLTATWESVSKETVSKIQQLENSLSVWLKPDPNTTAFTNICSSINSACVPHFSILRTVLSQIDNKIPTKTADGNINVDKLRTVFGIVVELQRLQQQRNYTMKPTKLFIQLQDINTLSMDELADLSLKCEAPVSKAKKYNSPEQVVQQDWRDQIKKTFAKPLVQTSVGIDLPRLASSFTFRENAEDVSINNGNKIQDINNVLLSLASITSDSGLNEKIKEQFNQYIPASTDPDSDFKRDLIRFLDEVCESDNSKLVNILKCCNQAIIAPVVIELTLNIAKAVPFMDAGGWRILISKTNPSLISVIENANTNNNSGHESNDDSTTTAASATEEKQEINSNNNNSNNNNSNIYVRHYKKQRSRSSQTKDYFEFEWFLQIELDQTCKNILSFDLKVSSLIFSTDTQATIRDPLLESFKDYIVSQDCIQYINLKNNSNESKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8C6</accession>
    <name>A0A8J4V8C6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_003813</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="AJWJ01000124">
        <property type="protein sequence ID" value="KAF2074894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8C6"/>
    <dbReference type="OrthoDB" id="15313at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001127">
        <property type="entry name" value="AGC family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000527">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="175"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="433"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2074894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="624" mass="70254" checksum="26BA2FAE3BEA56C0" modified="2022-05-25" version="1">MLKDIYNSLLSKKQNEQWNSVMLENQAQLFFSLKSIKGLKFEEPIKIYCVTSLEKQSIQTSTLTISDHSNTIEWNEGYTFDVMSSNSVLVLSIWQSCIQNNCNHNHDQQMDITPNNIKGLNNNGKLIGKLSIPYSTFSRQYLDQWFQLSGGNNSTSVHLKLSFTNSNKKVTVNDFVFLRMIGKGAFGQVNLVKKIDTGRLYAMKIIKKDRCLDMNAVGHTFTERKLLKKYYHPFIVGLKYSFQTQDSLCMVLDYIGGGELFYHISERETFSEETARFYISQIMLAIGFLHEHGVIYRDLKLENLLMDLDGNICLVDLGLCKEDVQDGRPTYTMCGSPEYVAPEIITGTGYGRSVDWWALGTLFYEMIAGLPPFYSEDPHEMSQFILSAPLKFPPNISKNAASLITLLLNRDPAKRLGSGDSDVEEIKAHPFFRNLNWSKLLNKEIDPPFKPHLIGPLDLSYFDPVCTIHSQPANPNTINTNLSESDQMAFQGFSYSAPDIFIYNNTTGAGSANGMMLSNSPNPSRSTPPMSPVSPVSPRNYNNTGMSPINFSPVHSPLVNRNSSNNNGNGNGGWVHPQYPNLQQPIPQKLPLLSKLQESTFERRRSLSVNAQTQFTLALSPDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8R7</accession>
    <name>A0A8J4V8R7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chromatin target of PRMT1 protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_000628</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000012">
        <property type="protein sequence ID" value="KAF2078077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8R7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025715">
        <property type="entry name" value="FoP_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13865">
        <property type="entry name" value="FoP_duplication"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01218">
        <property type="entry name" value="FoP_duplication"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Chromatin target of PRMT1 protein C-terminal" evidence="3">
        <location>
            <begin position="70"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="74"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2078077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="144" mass="14792" checksum="572CAFA18BB33638" modified="2022-05-25" version="1">MSQKNSRRVIINGGAAGGSLNDRFTQMKSSPKPGMSSPTVKRTRDSSSRGGRGGSTTITISRDANKPLGTGSPSRGGRGGFRGGRGGASSRGRPTGGARGGRGGFSRGGRGGRGGRGGKREKTPSKEELDAELDQISENNQMKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V9D4</accession>
    <name>A0A8J4V9D4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RhoGEF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_000003</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000001">
        <property type="protein sequence ID" value="KAF2078632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V9D4"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.950.10">
        <property type="entry name" value="Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051092">
        <property type="entry name" value="FYVE_RhoGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003096">
        <property type="entry name" value="SM22_calponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003128">
        <property type="entry name" value="Villin_headpiece"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036886">
        <property type="entry name" value="Villin_headpiece_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673">
        <property type="entry name" value="FACIOGENITAL DYSPLASIA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673:SF269">
        <property type="entry name" value="RHOGEF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02209">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00888">
        <property type="entry name" value="SM22CALPONIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00153">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47050">
        <property type="entry name" value="VHP, Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51089">
        <property type="entry name" value="HP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="4">
        <location>
            <begin position="32"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="3">
        <location>
            <begin position="238"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="450"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="HP" evidence="5">
        <location>
            <begin position="881"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="592"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="598"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="641"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="670"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="704"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="736"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="752"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="769"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="793"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="815"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="835"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="847"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="868"/>
            <end position="880"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2078632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="942" mass="106209" checksum="5AC12C3564458DDF" modified="2022-05-25" version="1">MEHRPILRGTKSNFSLAYNIKNITDERVKQYLRTEIVVKEWIEKVLQIKLKDNLHESLQNGIVLCYLANAIEDNIVPTIRENTTVGFKLKENIEFFLMAIEEIGLSKNSRFQTNDLFEAENLVRVVESLAALARFVVAEKGFHIPLRPITNTINVEMPKGEGLKVLKILLSQIKEIPDKSTSGYKPKGAAILKAQMALAAGKSIDFGKCEKGFVAFQSLWRGFKVRQMVKKIRRDIAYREKVAQEIYKTEDDYVKSLSICISGYLEPLIKSNLITKDQQKLIFSDIQIIYAFGKRLLDLLKPRTEKWFIYQKLGDIFLIIADFLKVYTSYVQNYNSSLETLEELKKKEKFLNLLNECREAPAVKNFDITAFLIMPVQRVPRYYLLLQDLVKHTWIDHPDYEPLKKAADKMKDIAAYLNEKKREGENFIKFTEIQSTLVGKVPQLFTPSRRYIRQAHFINVQKKTEIVIYLFNDCIIYGKPAKGGIIGGNQKKVKYLGIVDLPNTTVHEDPLPNVAFHIRSTTNALLFTAYPKNEDERVEWVAEIRKQIHAIAESQKIKETKLNEQQAQLNNPTVKPDPNQTVEQQLQERKKTRKFNLDDSLSSSQSNSLNSSGTNHSTTTTTTTTTTTATANSEPQQPQQNNTLSSSPSTATTSPTSPNPKEHGGFISSLFGNSKSRKSTGGSITPVSSTPTKDKDGAIHQEQVPDSSQPLSAPNSPTRVDSDSVLSPPSSAEKGHRPKKNKKHGISTTAVSKDDSEIGIKKNADGTRARSQSRLSRLTGTLTFRRKKDKSLDLSGDGDSGSNNNSNDERDDHQNINSNTATTADESQSKDSLHTSDSVSTDLSVSANNQENTGGTSPYLQSSSPTHSNRYSSSSFNSDVDSNKGPLTFEEIQQQKGDLDILQLETYLAEDEFKKLFNMERFEFNKLPKWKKDQKKRDLKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJP3</accession>
    <name>A0A8J4PJP3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">rRNA-processing protein EBP2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_010582</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EBP2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001214">
        <property type="protein sequence ID" value="KAF2068092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJP3"/>
    <dbReference type="OrthoDB" id="443772at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008610">
        <property type="entry name" value="Ebp2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13028">
        <property type="entry name" value="RRNA PROCESSING PROTEIN EBNA1-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13028:SF0">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN EBP2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05890">
        <property type="entry name" value="Ebp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="28"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="208"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="381"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="98"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="224"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="390"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="412"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="465"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="492"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="511"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2068092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="530" mass="59929" checksum="3430085050CD6DB6" modified="2022-05-25" version="1">MSAKGITTKKAPVAAVKKTVVAAKKVVPAKPVAAITKSTPVKKSTTPPPAKKSKIVVQQPESESEDDEDEDDEEYDQEEEDDLKKQLAAMISAGGASFDDEDDEEDDEEDDDEDGEDDGVIDFDDDEEDDQEDDGEEDQDDEEDVEAEVDDLFEELEEDEEEYQLDSEESEEEQEPVAIPTKKLNKKVEIGSTAFQGFASVADIIKQEASKPKKPAPAIRELTAEDELEIEKSDLEEDDEEEEEEEEQEEKQTKPQTTNKYFVNDTEGLQLKLRELKYNTVGKAPWIHTLAVTSSNSLEVSDIHNDFEREIAFYKQSLESVTECERLCKENNLSVRRKPDFFAEMVKSDEQMLKIKSKLASEKKRVETAELIRKKREIKKFGKQVQVQKLQERQKQKSDAIESVKKWRKNREKGNTSDEYKIDLEHEPKDKKRKRDERDSLPTKSDKRKKKDTKYGFGGKKRYAKTNTSESTNDFSGFSVAKNNADRDYSNGKGGKMNKGGKGGKGGAKGGAKKSASRAGKSKRGNTKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJT2</accession>
    <name>A0A8J4PJT2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_009788</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Probable transcriptional regulator.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bZIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000806">
        <property type="protein sequence ID" value="KAF2068892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJT2"/>
    <dbReference type="OrthoDB" id="19883at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14686">
        <property type="entry name" value="bZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14312">
        <property type="entry name" value="CREB/ATF BZIP TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14312:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="9">
        <location>
            <begin position="416"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="22"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="107"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="160"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="204"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="252"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="824"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="441"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="107"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="122"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="204"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="229"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="258"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="324"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="358"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="374"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="396"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="824"/>
            <end position="836"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2068892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="863" mass="98881" checksum="AA5E2C98B1997C4C" modified="2022-05-25" version="1">MEEFESFFSQLIPAPDQDIWMLGAHPGKQHEGAASGGGVEGSGIPSGWSLNSSLQNLGTSIEDPQIRDQIQQIQQQQQMQQQQIQQQLLNYQQQQNQHFMNRKQQLEQVPNYQQQQNQQQQHPLSNIIDTSSFPSSGEEPIDVYQIQQQLQQLQQQFQFSSQQQQQPQQQPQQPQQQFAAPIQFQQQQQQQQQQQQQQQQQQQHLQQLQQQQPSQQQQANNNNNNNNNFYNPTNSGVSNINQQFYNINSQFNNVSNQSKSTPPTNNINSPSSSPYLAASNNNSNNNVQSIPPMTYQLQPPQQIEQPQIPKQKEQQQQTISSLINNNNNNNNNNNNNFNNNNNNNNNNNNDNKSTNKSNKTKQPKPPKQSKGKKTPSTPLSQPTTPSSNINTPVSMPTPSSTNSDSPPLDDDEDSEDKKKVVNRINQNLASRNYRQRKKEYIKEIENKMAALTFENHQLKKENESLKESGGVEVMRPEPELITMLMEGKQIIIQLSQTLKKNDERTLIYLLHLFHCAIAKRYAITEKEVEKIVHPYTQSKLAAMGYVPKSDNFLNCIAGPASEGWFTLFKQEAGVNEKQSEQLETLRDQYQKIDMGLQMEREQLDKEIKQFYYTKILILPNNPLEIGDIPSQPYDINPILNSPLEISQLLDFARKLESLKRNFILHRNLMLDTLASLSSILTPRQEALLLVRVHFYTSYDIAHMELLKDVWTNIVSQNKSFTGPMNIAEALRKMSESTNTPPSIENIVKPPQFHQYTPKSLPSMKKSQIQESIEPLNINVSNYDNNIINSIINSNNNNINNNNNNNNNNININNNNNNNSNIINSNNVNSNNNNNNSALPLPTAESIPYNTSSDKKYHWYNYEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKI8</accession>
    <name>A0A8J4PKI8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010526</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001172">
        <property type="protein sequence ID" value="KAF2068149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKI8"/>
    <dbReference type="OrthoDB" id="1746725at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051014">
        <property type="term" value="P:actin filament severing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008154">
        <property type="term" value="P:actin polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.950.10">
        <property type="entry name" value="Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007122">
        <property type="entry name" value="Villin/Gelsolin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003128">
        <property type="entry name" value="Villin_headpiece"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036886">
        <property type="entry name" value="Villin_headpiece_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977:SF51">
        <property type="entry name" value="PROTEIN FLIGHTLESS-1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977">
        <property type="entry name" value="VILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02209">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00153">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47050">
        <property type="entry name" value="VHP, Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51089">
        <property type="entry name" value="HP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HP" evidence="2">
        <location>
            <begin position="65"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="134" mass="16091" checksum="B84992F82A6122A5" modified="2022-05-25" version="1">MSMELATEYSATLTDGRKNVPVFVIYYGKEPYIFTCVFHGWDFSKRILPTISFDKDIISAKEILDLYTKRYSYDDIVNKPYPKGIDGSRLEEYLPDEEFMKIFRMTLSDFQRLPLWKEQTWKKELRLYNVLEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKL9</accession>
    <name>A0A8J4PKL9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_010148</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May act as a GTPase-activating protein for Rab family protein(s).</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000946">
        <property type="protein sequence ID" value="KAF2068525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKL9"/>
    <dbReference type="OrthoDB" id="27140at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000011">
        <property type="entry name" value="TBC1 domain family member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF337">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="5">
        <location>
            <begin position="202"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="605"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="778"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="54"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="637"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="778"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="796"/>
            <end position="857"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00043879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2068525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="936" mass="109342" checksum="A45BE0763821841E" modified="2022-05-25" version="1">MDIFDQPTPFDDVDQIDDTKYQQHTSGNSNVNIFIDDDNDVIDYDKQQREQYDIEQEQQEKIQQQENLERERLERERLERERFEMETLERERLEIERLERERLERERLERERFEIQERLRIEEELRLAEIERQRQIELERIREYEEMKSKTVLIQDQEIPLLEFNGRIKKWNDDFFEIVKKNDDNNESPFTFDNSKGSFGLNKDHTARGIYWRLCLGTLSGDSNTWVEKTNNQRKKYDNLMKSYIVNPRDSFTTDISDDPLSQSENSLWNQFFDNENQQKEISHDISRTYPGTPFFERDDIQNIMIRILFIFSKQYPKIKYLQGMNEILAPVLYSVYNDSHWFDNKEVFVKQSHDFNRRRGNSNNLKDISEYFKNRFMPKNAIPYSTLLSNPDATKVIQENTSIDPQGDGLESFLRDPKYFEHDSYFIFESLMNIIGKWFTSPPSSPQPPPRVQGKFKELYDIREKEASDQAINIVVVDQCLKMFDLVQLIEPQLYSYLKDLSIEPHLYSLRWIRIILAQIFPLGSLLLIWDATFKENVIDLLPFICVAMLVNIKDNILEKDYSECLQVLFHYPISNDIDQLLESSYSIRDRIYSIKKQNGLISKQQSTPSIPIRPTPRVYPTTFTQSVPPPEPIVKKSNNTSSFNNSNKSIENNNNNQQQQNKITQQQPPQKVQQVKVQQPHSQQLQQQQQQQQQDSFGGLFSSAVSSFKQIVSDLAVIDSSWDVEVLRENQTHVSNRLERLVYILETIKSGENYETIESVQDEITAIRGVLMGNSNSKKLNSPSIDNPSNLSFKSVTHKQQQQQQQTTPVLSSSSSSSSSSSFKSNKTPTTSPKPITNNSNNSSPTLTSSSPTFNGVKQQQTKDDFLSSSPDFFIDNRSTPSVQQLKNLSLPTVDLHEDDLVEVITKDTTANEFDDELFDQINFKEQFGSKMGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKR0</accession>
    <name>A0A8J4PKR0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_009747</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000794">
        <property type="protein sequence ID" value="KAF2068934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKR0"/>
    <dbReference type="OrthoDB" id="193499at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12347">
        <property type="entry name" value="RRM_PPIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034168">
        <property type="entry name" value="PPIE_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48037">
        <property type="entry name" value="ATPASE E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48037:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="14"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2068934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="126" mass="14267" checksum="EF67ED7FDFB11659" modified="2022-05-25" version="1">MTTNTETAGINKKKTLYVGGLDENVTTDILKAAFIPFGNIVEVLLPLDYKTQKHKGFCFVEFELPQDAADALDNMHESEIFGRVIKCSIAKPITINKNKALWSSDQYHWKEEDADMIIDQDEITTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKW0</accession>
    <name>A0A8J4PKW0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009915</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000851">
        <property type="protein sequence ID" value="KAF2068763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKW0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="203" mass="23928" checksum="494AE530956E17F8" modified="2022-05-25" version="1">MPGPIDDEINRIIQHDKNGNYLVQWEGYENRKQWIYKEDISPDALEEYHRERGDGIWLNSRIDKNKIIENDNSKNMSQDNDNNKLQVNSNNNSDNNSQLQIDRNSNTINKQQETRKNNNIGFNNKFININNNSNAISIIAPKPNYNQALENRDNNNISVRKVNKNYQNSYVPNYSSSHVPDYRKNKYRKKTRFSGKYKIDDSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKW7</accession>
    <name>A0A8J4PKW7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_010271</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001008">
        <property type="protein sequence ID" value="KAF2068404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKW7"/>
    <dbReference type="OrthoDB" id="7851174at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23792">
        <property type="entry name" value="UBCc_UBE2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000010">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2-16 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="7">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="5">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2068404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="147" mass="16401" checksum="3AB80C9398D1CA7F" modified="2022-05-25" version="1">MALKRISKELQDIARDPPSSCSAGPNGEDLFHWTATIMGPKDSPYEGGVFFLNIHFPTDYPFKPPKISFTTKVYHPNINASGSICLDILKEQWSPALTIPKVLLSISALLSDPNPDDPLVPEIANMFKSNRAQFDANAKEWTRKYAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLJ9</accession>
    <name>A0A8J4PLJ9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009868</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000836">
        <property type="protein sequence ID" value="KAF2068809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLJ9"/>
    <dbReference type="OrthoDB" id="24190at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="596"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="597"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="619" mass="71107" checksum="A72A24D6F388F94C" modified="2022-05-25" version="1">MDDNLFNLVWRNQSIFSTIKQHLFYNGLRYNGFRKFVKDVKYLKVLPKQVHVYVEGFYTDFIGDLEKNLISGVSINHSSQLEFVDHMPPVEKIVFLNLKELETLVYPIPDTVVLVRFVYFDGVHYKAAIEKLPASVKSITFDFVSREEFVIPFLPSFVTSLEIGKLSQSIEPGFLPPNLHTLVLNDFNLPLQNLPQTITHLSLLRFNQPIQPNTLPVGLKTLIFKFFFNQEIQEHSIPSGVTCLALGESFNKVIKPNTLPASLLTLDFGGSYNQALEIGSLPPLITNLNFKDSFNQPIGVSVLPPRLKSLDLGFNYWQRIETDALPTTLVSLKGMEPNSNYPFEKLVHLQTLSFQSFRKSRQIKLPDSLKALDITPFKRVEFEFYPNNLQELVFDYWGKYPLIPGSLPRVNKLTLTLRDSIPLTKGVVPNGVKTLILELEGSNCKIPRGSIPASVTKLKFKKLHYQLDFESIPNTVRYLTLPVGFKQSLQSRCCPIPDNIIKLKIHIDRVYNWNTDPPCDAEKWNIVYAGLNKFINRTDQQDKFDYAIVLTNFHCSYYGPRSDQQRYFDLINDYMKRPSASDDILKDLVLVQKEDSDQYDDSDVDQEEQYFTCSSSDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMC6</accession>
    <name>A0A8J4PMC6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008975</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000610">
        <property type="protein sequence ID" value="KAF2069708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMC6"/>
    <dbReference type="OrthoDB" id="410198at2759"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00529"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050680">
        <property type="entry name" value="YpeA/RimI_acetyltransf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43420">
        <property type="entry name" value="ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43420:SF42">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13673">
        <property type="entry name" value="Acetyltransf_10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="2"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="142"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2069708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="277" mass="32925" checksum="C08710FC3FA669F6" modified="2022-05-25" version="1">MKTITIKNSIPYSDILSLRQKVFLPNDTDLELVKIKEDPSAHHFGIYDDDNDLIACITLIIDEINIRFSYFSVRKDKQKLGYGTYIMNWLINDYSKSNNSKRIWCNSRTTSNQYYKRFGFRDTGKSFIRQSDSVEFIVMEKILYSPPTIPYDQVLLMRQKVMYPNKDLDFVKLQEDHLGKHFGIYHNQELVSVMSIFINDKELQFRKLATKLEYQSKGFASILMVWLIEYSKEKNINRIWCNSRKSASQFYKKFGYVETNQTYTKNDIEFIIMEKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMG1</accession>
    <name>A0A8J4PMG1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008880</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NmrA-type oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000593">
        <property type="protein sequence ID" value="KAF2069798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMG1"/>
    <dbReference type="OrthoDB" id="17353at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051164">
        <property type="entry name" value="NmrA-like_oxidored"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42748">
        <property type="entry name" value="NITROGEN METABOLITE REPRESSION PROTEIN NMRA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42748:SF7">
        <property type="entry name" value="NMRA LIKE REDOX SENSOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="3">
        <location>
            <begin position="18"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2069798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="325" mass="37001" checksum="95872E6D9E7AD622" modified="2022-05-25" version="1">MNNKTQPPIFVVPQVFSKQGKSIVNAILRDGGKYQIIGLTSRDVQKTAQADLLEKRGVKVVQVDLSDKDKLVEIYKGAYGVFIVKPVISPFDPEFKEKSFDLIKTQADACLEANVQQVIFSATDGELDSNGKTRDQRYIEGLSIPFVTTFFLGFFYSNVIEFYRPKVEYIDDSQSPTIVFSQPFDDTTAVPYVDSYTATGQVVSSILANPHQYNRHSLKVVSEYLTGPQFAEIFQQSTGIKSRYQTIDCQQYLIDNGFHASPEYQFIGTALYNTWLQSVINTHRFKDQTNEYHCTPLLNLNQFLLSTKWNGIDSFDNFKLNNQYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMI4</accession>
    <name>A0A8J4PMI4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DJ-1/PfpI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007991</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C56 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000458">
        <property type="protein sequence ID" value="KAF2070690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMI4"/>
    <dbReference type="OrthoDB" id="543156at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03169">
        <property type="entry name" value="GATase1_PfpI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006286">
        <property type="entry name" value="C56_PfpI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01382">
        <property type="entry name" value="PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42733">
        <property type="entry name" value="DJ-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42733:SF2">
        <property type="entry name" value="DJ-1_THIJ_PFPI FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51276">
        <property type="entry name" value="PEPTIDASE_C56_PFPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DJ-1/PfpI" evidence="2">
        <location>
            <begin position="4"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="196" mass="21491" checksum="39A6E22B0BFA518E" modified="2022-05-25" version="1">MAPKKILIITGDYTEDYETYVPIQVFQLVGHTVHIVSPGKKNGDYVISAVHDFLPGEQTYTELRGHRIAINYNFDEVNPSDYDGLFIPGGRAPEFLRLNARVIEIVKAIYERKAVIGTVCHGPQILVAAGILKGVCATAYPACEPELVLGGCSFKSIPVDQHVLDKEHKIVTGPAWPSHPAVLRSFLELLGTTITP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN19</accession>
    <name>A0A8J4PN19_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Arylamine N-acetyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arylamine N-acetyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000674">
        <property type="protein sequence ID" value="KAF2069381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN19"/>
    <dbReference type="OrthoDB" id="17550at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2140.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001447">
        <property type="entry name" value="Arylamine_N-AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053710">
        <property type="entry name" value="Arylamine_NAT_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786:SF0">
        <property type="entry name" value="ARYLAMINE N-ACETYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786">
        <property type="entry name" value="N-HYDROXYARYLAMINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00797">
        <property type="entry name" value="Acetyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="12329" checksum="855B7348BB4DC136" modified="2022-05-25" version="1" fragment="single">MKQDYISLFLDHIHLPLDFKVNNLNDLGLVMKATQVYVPFNNLEILNGTYGEVTRESIIQKVLIEKTGGLCYHLNLVIYYYMQEIGLDCFYVKVLPSDKNSHLNHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNL1</accession>
    <name>A0A8J4PNL1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Complex 1 LYR protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007967</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000455">
        <property type="protein sequence ID" value="KAF2070713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNL1"/>
    <dbReference type="OrthoDB" id="15893at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35763">
        <property type="entry name" value="COMPLEX 1 LYR-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35763:SF1">
        <property type="entry name" value="OS11G0133900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="2">
        <location>
            <begin position="9"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="126" mass="15258" checksum="147A4B020D0DEC97" modified="2022-05-25" version="1">MTTQSSMVLFRRLLREGHRYQEYHHNHWWRNQITATFRENRDVKDPNEIKRLQDIARAYRYNIKSSRDLSELLDSYNIGIASRARIEKSSQRVGLKVPEWPEDRDKRIKERKEKEAQDQNNNSNSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNS8</accession>
    <name>A0A8J4PNS8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Diaminopimelate epimerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008115</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000474">
        <property type="protein sequence ID" value="KAF2070568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNS8"/>
    <dbReference type="OrthoDB" id="4768at2759"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00025"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008837">
        <property type="term" value="F:diaminopimelate epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.10">
        <property type="entry name" value="Diaminopimelate Epimerase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00197">
        <property type="entry name" value="DAP_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018510">
        <property type="entry name" value="DAP_epimerase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001653">
        <property type="entry name" value="DAP_epimerase_DapF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00652">
        <property type="entry name" value="DapF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31689:SF8">
        <property type="entry name" value="DIAMINOPIMELATE EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31689">
        <property type="entry name" value="DIAMINOPIMELATE EPIMERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01678">
        <property type="entry name" value="DAP_epimerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54506">
        <property type="entry name" value="Diaminopimelate epimerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01326">
        <property type="entry name" value="DAP_EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="39115" checksum="D5586FF40B06CE9A" modified="2022-05-25" version="1">MEAGGSSSDSASVAGDISFNTSPKYKVKFTKMHGCGNDFVVFELDKVRQLSTNEYVRDWSVQQLSDLSKALANRKLGIGCDQVIIVNSKPNNTDTDYEMLIFNSDGEQATMCGNGVRCFSKYIIDNQIQSQTKNTSNEIQNIETLAGVIKTFPQLDHPLNTSNTTLIKVNMGNAMVLNTKQTVNAIARNEINLSTTDRSLYVYNIEIDHPTVNRLECVLVSMGNPHCVVFIERNQELGNISGGNYSGARLALMEIESIAMALQKHPLFADSCNVEFVTTASGKPNKVLSRVYERGSGETLACGTGACGVAVASILQGHCNADSEVFVEMPGGLLLISWKGHNQVFKTGPACTVFSSTIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP18</accession>
    <name>A0A8J4PP18_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_007161</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000366">
        <property type="protein sequence ID" value="KAF2071517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP18"/>
    <dbReference type="OrthoDB" id="449052at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02205">
        <property type="entry name" value="CBS_pair_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF25">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="17"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="106"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="182"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="257"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2071517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="312" mass="35233" checksum="0709816473753B8B" modified="2022-05-25" version="1">MELLRTLKASSPVFPNDDSKVLSVNKDDTISKAFQVLIDNNILSVPVYDKNEKKFCCFFSMLDIIQEILDVTNNFKLPDGDISSVLTILQEKQLFKMSKVGDIANISKRDPFIIVNSEMKLDDVARVMVRNNIRRVPVLDSRGELCNVITNSRLIECVSHLFGMDHELTKMGQQTIAQLNIINYDVISVRSDKGAQEAFKIIAEMGVSGVGIVDQDNKLIGSINDSDLKLIKSHAQYLSLLYLPISQYLQVLEELKMKSKQILTFKETDCFKDVLENIVATKSHRLFLVDDDFKLKGVVSLQDILEKIVISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP67</accession>
    <name>A0A8J4PP67_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">IPT/TIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_009177</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000650">
        <property type="protein sequence ID" value="KAF2069499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP67"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052014">
        <property type="entry name" value="Dictyostelium_Tiger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31341:SF10">
        <property type="entry name" value="IMMUNOGLOBULIN E-SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31341">
        <property type="entry name" value="IPT/TIG DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="117"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2069499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="156" mass="17373" checksum="EB25561CD38B771E" modified="2022-05-25" version="1" fragment="single">EKEIPNPNNLLFSFDAPKIESYISYLIGNGSIVTVFGMNYHNPVLVTIGGVECHFPNSTDSNTTTCFLPKFDSDFETPEDGNLTIHILVGGQTTESDIFVFNEAQRNDPPPASKMKWLIPAIVIPCFLALLCAVAVTIILVKRHKKMKALRKMFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP76</accession>
    <name>A0A8J4PP76_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000353">
        <property type="protein sequence ID" value="KAF2071650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP76"/>
    <dbReference type="OrthoDB" id="23586at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="502"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="532"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="560"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="603" mass="67423" checksum="E03925D759DEA19E" modified="2022-05-25" version="1">MIGGILDNVFDTLGGVLDLTTGVVESAPTDLVAFVRKLDPSLTLPQAIELVTTTLQKLTLNRLIDLTVLDTLNKQKDKVRERSDRIFLENIQYHLDTNVLETLKWKKPTLDQDRLAMISGTKVKEWYTKYASLWVVLLTDYELPGKVKSDLIQADMQQMAATKIFTSQMTKIYNYAYLEINPDMAFYMNTAAAWAPLLTDYYQSEEYLEFWIDKVMIQLQDDRLFQFYKDLLASYPIGVKFVDTLQNGNSSLDPICSDIRAKLDILDPTCRSSNKVIGYLQQVALTVHLLEGGLNEKFEENLEQNLSILFNSYLEIENPNPEQSEIIEFIKGLIAISGGQPAGAITTAADIIKSANKANKQNLIAGGYKPKSIKKYHMFEAEMMDSVINDHKLGGELTKLAKYRNMFCGIWRGCQAMVLLLGVVNYDTDAPIMTQVQFVGDSVSLLADFVTAFDSNNKYLTSKFSNFGKLIGRTFSKFTPARLGDFFGKNLTKIFTTSAADFISFRVCPILLLVACYNNILDAKTSIEEKNFPGLVCDLGGLGCNLVSAGILLLGVPYAALAALIIGAVAIGFFLVKIIFFPAKTPIQKYYESSLFPLKYKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ26</accession>
    <name>A0A8J4PQ26_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Reelin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007908</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000447">
        <property type="protein sequence ID" value="KAF2070776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ26"/>
    <dbReference type="OrthoDB" id="16296at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035169795" description="Reelin domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="279" mass="30008" checksum="C714534B6EADCB66" modified="2022-05-25" version="1">MKIQYLLGFIACLVMIEYVAANAGLAKPIPRVSCDLKNKKGCSIKSACAKRNPNDAVNPIYISESYDIAIFQKLSFHSSNSSNTYTLQYYPDKTGNVTETAANTGIQLITFNQNDMNANNSMYAVKLDPLATNEKLMAEISWDEEGYSYGYAQLTFDTRSGSGTSFYSCSNVRLQKTKPKASNSTATVQVDSTLTITDGEGNSLGNATISANGGTLSIPSMVDPDAKTKSSQEQEIGGNTQPAAGEEEEHSAGYSIQPPASPIFYLGACLTIVISFLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ64</accession>
    <name>A0A8J4PQ64_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_007097</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000360">
        <property type="protein sequence ID" value="KAF2071588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ64"/>
    <dbReference type="OrthoDB" id="1939479at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016929">
        <property type="term" value="F:deSUMOylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.395.10:FF:000001">
        <property type="entry name" value="Sentrin-specific protease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606:SF141">
        <property type="entry name" value="GH15225P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606">
        <property type="entry name" value="SENTRIN/SUMO-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="6">
        <location>
            <begin position="570"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="128"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="255"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="406"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="52"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="138"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="169"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="298"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="414"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="428"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2071588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="769" mass="88555" checksum="E457889E5182138F" modified="2022-05-25" version="1">MSSMIIETSPAPPTPTHTSTPQQTQQTRSSILFQEVGNIINTFKRKTFDLISPSSSQQQSEDINNNSSSRSLKRSKYYYHKQNQHSQQSPQQQQQQLSSNSIFSITVFSTKSFLGKLANSIPSALSSFSLGRSKDNNKSSQKNKSNNTTVAKDYSYYNRVDNKYTLRRSGNSNKNNSNSTTSNSYNQQDIINSVRLKKLLYRPKQQLPYQYQGDFNNIHLNFAPSPIKNQYLQQKEHQEQQQKQQQLFNNSHYIMDESNTDTDNNEIANGNHNNSNGDNGDNDASFTKHNSTPKKANRPTTPSSSNKLSFVDSVAIEKQNQKFIKSLDNVINKCQTSFYVTGQPADVDDDDGDNSNILSTINNNTSYNVSTIPQQQQRTSIFKQPISPYRSQQELQQQEQSFDLFNQDDDDDINNNNNNDLSLGDLNTSLSKTSPTTKYTSGASSLYSSRNSQYKNNLIASLRPNQSLNQDIVDRIEKQSQDPQYQFKQQVDVDLKKHRDSEIFSLLDKLDSLHKTNKWNILRQIDERIERDKNAKPILRPLSPKEDQIIDNIFKRGGDDEEVVSKVGANICTRSDIRLLGPGKWLNDEIINFYLIMLNTRQTTSTKYMKCHFFNTFFYQLLCNNNGTYNYDRVKKWTNSIDIFSLEKVILPIHLGNHWCCAVINFKEKLIQYYDSLLGDNPACLSKLRRYLQDEMKNRKKEGIINLDEFRDVTDKDIPTQNNGYDCGVFMCKFAEFSSRGAKLNFTQKDITQYRRRMALEILNKQTFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ80</accession>
    <name>A0A8J4PQ80_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphomevalonate kinase</fullName>
            <ecNumber evidence="3">2.7.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_007066</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GHMP kinase family. Mevalonate kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.2" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000357">
        <property type="protein sequence ID" value="KAF2071618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ80"/>
    <dbReference type="OrthoDB" id="10262935at2759"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00099"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004631">
        <property type="term" value="F:phosphomevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006694">
        <property type="term" value="P:steroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016005">
        <property type="entry name" value="Erg8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035102">
        <property type="entry name" value="Phosphomevalonate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31814">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31814:SF2">
        <property type="entry name" value="PHOSPHOMEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017288">
        <property type="entry name" value="PMK_GHMP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="12">
        <location>
            <begin position="178"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2071618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="499" mass="54623" checksum="4B590FFEAD12F42A" modified="2022-05-25" version="1">MSKSICCSAPGKVLVTGGYLVLDRLYDGIVFTIDSRFYTSIVSSPLLNNNNNKSNDDSVFICDIVVHSPQFKSSQSYHLFNNPNNENKLYQLIPFGNANYRENKYVENAIIYSLILASGYKDLQSSIGSLKLDITIQGDNGFYSQIPQLKERNLPISFESLKSLPKCLPVVGTLQELQKTGLGSSAALVSSLTGALLSFLGVIDLNNSNNNNLKDKTLLHNLAQVAHCVAQGKVGSGFDISSAIFGSQVYRRFSPEIISNILKLSDKQLYPSAKELIENVNFENSKWDNQHSDMALPPGLSLLLADVSIGSNTPSMVKSILEWRKNQHSTALDLWTKLNEKNTDVKKHFTQLHALYRQDAGKYNRELLACATVDPVQWSGMNSVGTVLHSVRESFVAIRGLMREMGGLADVPLEPVEQTRLADATMSIKGCVAAGVPGAGGFDALFAIIIDSNADSSVSQQVKQTWMSWKECQVLPLVLCENSSGILVEPAENAPSSKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQA9</accession>
    <name>A0A8J4PQA9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CN hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007011</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000351">
        <property type="protein sequence ID" value="KAF2071678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQA9"/>
    <dbReference type="OrthoDB" id="10250282at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07572">
        <property type="entry name" value="nit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045254">
        <property type="entry name" value="Nit1/2_C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088:SF27">
        <property type="entry name" value="DEAMINATED GLUTATHIONE AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088">
        <property type="entry name" value="NITRILASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="2">
        <location>
            <begin position="27"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="305" mass="34721" checksum="6625B44AC0D68343" modified="2022-05-25" version="1">MFRLNICKTTIAINRFMTTTTTTTNNKRIGLGQITSTNNKDENFKQCKRLIEKAVENKVQLFCLPECFAFISGGVHQFESRDNAEYLDQKGGIIERYQKLAQENKLWLSLGGFHEKIEGENEMIYNTHLIIDADGKIVSQYRKMHLFDVDIPSKGVKMNESKVVKGGNELVVCDSPVGKLGLSICYDLRFPELYLSLRRMNSDILLVPAAFMKSTGEAGHWKTLLQARAIENQCYVIAPAQTGNHHSKRTSFGHSLIIDPWGKVLSELDENTIDIAFTDIDHSYIETVRENIPVFNHKKLNNYKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR14</accession>
    <name>A0A8J4PR14_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UbiA prenyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_008014</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000461">
        <property type="protein sequence ID" value="KAF2070669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR14"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042371">
        <property type="term" value="P:vitamin K biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13962">
        <property type="entry name" value="PT_UbiA_UBIAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026046">
        <property type="entry name" value="UBIAD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929">
        <property type="entry name" value="1,4-DIHYDROXY-2-NAPHTHOATE OCTAPRENYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929:SF0">
        <property type="entry name" value="UBIA PRENYLTRANSFERASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="219"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2070669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="293" mass="33995" checksum="98F031CB51305DDD" modified="2022-05-25" version="1">MINFRKIQGYIHGSRFRLTMTLLSYPIVGMCLGKRDAGFVPFEYLYLLPVFMAHYSIANILNGLCDFEAKIDEKETSNDRTMFDYGLTSKDIWNFVYFDIAVMYGFSYLAFSKFSTLDLMYVFAYISFGIFLAIYYNLPPLRLKTKFFGAESTIFLIFVVQVNLSYFLITGDSSFSTCFIHFPGSILQIWDIMMNLYVDSEEDKRDGSRSIPIVMGDKFASSLIILTPILGCYLTYRYSVVYNNMYTCLPLLTIPLIYFVISLACKKQKRVKPYYFLTLFLYNTLFAIGLLMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRU0</accession>
    <name>A0A8J4PRU0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_006862</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000335">
        <property type="protein sequence ID" value="KAF2071825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRU0"/>
    <dbReference type="OrthoDB" id="7326421at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032797">
        <property type="term" value="C:SMN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024977">
        <property type="entry name" value="Apc4-like_WD40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056424">
        <property type="entry name" value="Beta-prop_GEMI5_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052640">
        <property type="entry name" value="Gemin-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056421">
        <property type="entry name" value="TPR_GEMI5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46362">
        <property type="entry name" value="GEM-ASSOCIATED PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46362:SF1">
        <property type="entry name" value="GEM-ASSOCIATED PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12894">
        <property type="entry name" value="ANAPC4_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23775">
        <property type="entry name" value="Beta-prop_RIG_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23774">
        <property type="entry name" value="TPR_GEMI5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="115"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphase-promoting complex subunit 4-like WD40" evidence="5">
        <location>
            <begin position="177"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Gem-associated protein 5 second beta-propeller" evidence="7">
        <location>
            <begin position="435"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="611"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="673"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="716"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="domain" description="Gem-associated protein 5 TPR" evidence="6">
        <location>
            <begin position="895"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="786"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="790"/>
            <end position="826"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2071825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1086" mass="121355" checksum="958D96C35096BEB6" modified="2022-05-25" version="1">MSLVPDQSYESTDNNEIEHKHEVIGDVNVNQSKRNVDDGDGDGSVSVNQAGQHESVIDQVKSKLKVSNHVEMLPCSSNWYSCMISDYNHNDIYIYASKSIIILFNIKTRKFIGELIGHSDRLTSLCFNKFNNQQCISGSCDKSVRLWDIDSCKCIAFHQELHRQPVTFVATSPLVLNLIASADKEGKIVLWRTLNNHTDIIAPIANTEVTFMCFSPNHADLVAIGYVNGVLVIYNLISKSIVTKISAHQQDVLSIVWFDNRVTKNMISGGSFDTLEYIATSSKDKTIKIWKQIDTNTIDKGFSNLAQLNPIKFSSMVNEKSANQQRSWVTLSWSSTHNPQYLVSSSATYDILLWNLQDLKAKPEKLKGGHSRTVFNITQANDPAPKHNNPKANNKYNNRLITISLDRNMIVWENCKAAFKIPGLGGFVYSLDSSPFNPNTLAIGCGDNSIHLWNPMENTDNPYECKSVWKGILSKVTALSICRHVIDGSLIAFGMDDGRVGVYNGNSNQVTMFPSGHKSEIYEVAWKPAKISQDRTKESKLYSVGNNEIYEWDLDNLDKGYLPLSHYVKSGFSGKQKSDIAWSEDGERVAISYSDGTIDIFNKHWEFVAKTKSHSKLVNRLKWNPHNSTLLASGSVDKKVIIYKLSPVAANQNNDGEKENVNSNFEIQVLSTFTGHRGSICGLCWSTSDKNLIASASGDGSVRVWNIETNECLAVIRGHDGRVFTVFWSLLDPTILFSGGEDQTVRMWDYTNPVFKPSIAESNTITPKMAPFNNIVKDSNDTIKPIELQSNTSPNSTSTPNSTPSSTSSSPPPSTSTTTTTINTPSQAPPKKKIIIPLTKDIIQKPDVKSQLLPLTKYYLGKLSSNSNDQYKDFIVDYGNTANNDNETIPSIESIFLDKCDVVKEQIEKESLELLKIDDIDNYLSIHMWNGNIKKALYYIIKHGKLTPNAVSLSMQAGKEIHETVCYLYSQQLVCVGDFHMAVSYLLLIGKVDEAIQVYRKSSHLHEAILLAKSRYPLDHPVVLDLLVEWSDLTSESNPLHSIKCLLSCLNKENKDKLFPEILHLLKSIKSSAKISEIQSLIENSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRU3</accession>
    <name>A0A8J4PRU3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000270">
        <property type="protein sequence ID" value="KAF2072548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRU3"/>
    <dbReference type="OrthoDB" id="10618873at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035295900" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="26935" checksum="4B59DF1A8E27619C" modified="2022-05-25" version="1">MAKVLNAFLLTLLINACLVGFINGKIVNPINGEEIKDYSMCCETETPVGETFLRSLQLLNTPLGSGENATSGSASAERVTELYFPHLIFSNGSNPYKFQSELCESNENMTEFTYPEFDLFSTADYEYECVIKNATLFDKFMMNQFILAINASCPDCNCVLKQSTDGYVKGFKVQIIGHPLVDDESVVTHYSILGCDITVETSNEPYKEGDIFTQTVQLVSSSNRMIANVSLLLISLFVMLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS99</accession>
    <name>A0A8J4PS99_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CBF1-interacting co-repressor CIR N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005767</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000239">
        <property type="protein sequence ID" value="KAF2072910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS99"/>
    <dbReference type="OrthoDB" id="2159131at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039875">
        <property type="entry name" value="LENG1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22093">
        <property type="entry name" value="LEUKOCYTE RECEPTOR CLUSTER LRC MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22093:SF0">
        <property type="entry name" value="LEUKOCYTE RECEPTOR CLUSTER MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="31108" checksum="AD935D0ABF64261F" modified="2022-05-25" version="1">MTHILKHKTWNPYSSKNKEKVERDEREHNEKLFKENQKKIRIESEKRYSQLKNNLKRKGDFDEDEVEDGHGDNIDQDERDTQSKDDKDNQQQHINLFQGITGDQIFSSKSKEKKLKQQIVSEGVRLGEGCIDNSPVWIKDNNKKSSSTGNKDDDNDIDERRKRKKEKREQWRLDREDPIHIVEKALGKENTKSSSSSSSSKTISTTSTSTVNKKKTIEELRKERLEREEKERKRANELKSTTSKTILGTGVPQSSVSFLGYSGGIKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSE4</accession>
    <name>A0A8J4PSE4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_005710</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000235">
        <property type="protein sequence ID" value="KAF2072970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSE4"/>
    <dbReference type="OrthoDB" id="2110130at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031288">
        <property type="term" value="P:sorocarp morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001135">
        <property type="entry name" value="ABC transporter F family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000104">
        <property type="entry name" value="ATP-binding cassette sub-family F member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF117">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="185"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="496"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="136"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2072970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="713" mass="80918" checksum="F69DF3DC383304E4" modified="2022-05-25" version="1">MVSQTNKQIKQVVLTTLGDKAKDIDETIIDYMTGVFEDEGVISDMDELVEILTPLLMDTCLIEDQEEVNQTINGLVDNLQKEKLITIKQKTSLSTLSQPVALQRLDDRVHAAVSWMKPEEAISILNKDSLEANEKRYNARKEARLQKEERKKLRQNAALAALNNLKEQQTMMASQIRGSNQSRDIHVEQFSLTYGKMDLIVNADLHLNYGRKYGLIGRNGMGKTTLLRHIASREIGIDNNLAILHVEQEVSGNEITVLECVLMADVERDNLLKEEARLNALPDNEKNHLSAKLQQIYERLNVIDAHTAESRAASILSGLGFTEEMQQSPTKQFSGGWRMRVSLARALFIQPDVLLLDEPTNHLDLFACLWLESYLVNWSKTLVIVSHQREFLNAVCTDIIHLNNKKLDYYKGNYAVFEKTRSDRLKSQQRAFEAQQSQKKHIQAFIDRFRYNAKRAKMAQSRIKQLERMDSISEVLDDPSVTLNFLEPEPISPPILQFQDVSFGYTPDKLLFKNLNLGIDMDSRVALVGANGAGKTTLLRLLCGELEETSGLVIRHGKLRFSRFSQHFVDQLDLSKSPLDNFLTKYPGTNPQVARAHLGKFGLSGDIALRTVNTLSGGQKSRVVLSQIAYTRPHILLLDEPSNHLDIDTVDALCQALNEFEGGILLVTHDERLISLVCDEIWYFDGEEGEPKEVKPFDGDWTDYKRQVWNLNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSL1</accession>
    <name>A0A8J4PSL1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005659</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000231">
        <property type="protein sequence ID" value="KAF2073043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSL1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="90" mass="10003" checksum="642464140B2A9E9E" modified="2022-05-25" version="1" fragment="single">MQSKKDENPPNANVGNTSGSNATKRTTFAKTVDNDNKDNNNNSNDINNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSX0</accession>
    <name>A0A8J4PSX0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">COMM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Scaffold protein in the commander complex that is essential for endosomal recycling of transmembrane cargos; the commander complex is composed of the CCC subcomplex and the retriever subcomplex.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000260">
        <property type="protein sequence ID" value="KAF2072651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSX0"/>
    <dbReference type="OrthoDB" id="10257479at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04750">
        <property type="entry name" value="Commd2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017920">
        <property type="entry name" value="COMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037354">
        <property type="entry name" value="Commd2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15857">
        <property type="entry name" value="COMM DOMAIN CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15857:SF0">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07258">
        <property type="entry name" value="COMM_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21672">
        <property type="entry name" value="COMM_HN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51269">
        <property type="entry name" value="COMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COMM" evidence="2">
        <location>
            <begin position="123"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00093300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="203" mass="23652" checksum="BD6D866AAF0529D1" modified="2022-05-25" version="1">MIIELDAKHKQDLLFLKTLSPEIVNEFCRIGLEFMKSGSNRKLYIGAAQKLNVSPDKIESVIDAITHLFSESSRYLLNENDFNDSLSPLKFEEELVNQLREVYFENRKDIRNVLQEFYPHLDHYSNLEWRLDTQVANRSLGQSINPIYMLKLSTKNGNVEGKGLKEFLLQTDPNNLKHITTELESALMEAKSYENRLIMRNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT24</accession>
    <name>A0A8J4PT24_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_005723</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000236">
        <property type="protein sequence ID" value="KAF2072957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT24"/>
    <dbReference type="OrthoDB" id="19740at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000087">
        <property type="entry name" value="DNA methyltransferase 1-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032563">
        <property type="entry name" value="DAMP1_SANT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027109">
        <property type="entry name" value="Swc4/Dmap1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12855:SF10">
        <property type="entry name" value="DNA METHYLTRANSFERASE 1-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12855">
        <property type="entry name" value="DNA METHYLTRANSFERASE 1-ASSOCIATED PROTEIN 1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16282">
        <property type="entry name" value="SANT_DAMP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="141"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="279"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="415"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="486"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="279"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="418"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="447"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="486"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="496"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="514"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="538"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="561"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="607"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="639"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2072957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="655" mass="74143" checksum="1361348CC9D1B328" modified="2022-05-25" version="1">MSDVRDILSGVIPGLASKSTTANSNTSGGAVASILSTPVKKQHKDTPKTIMEKLQQSFKNLEGETLTFSEPLTSLALKEKRKIKVNWKLRAFRNPVRTDQNLILYHWTKSNEKNNEYKYTKFNKKMDILMYNEEEYDIYLRDDSWTKQDTDLLIDLCKRFDTRFIIVADRFEGSVKRTVEELKDRYYKIQSKLIELRTKPEEDPYHNPLTTYSFNKVYETERKLQADKLFRLTELEVAEEEQLVEKYNNIEKHLLRHTKESKAVMKLSQVSLNNGPMKHYSQSDLLLSDPNGTRGANSAKKSKKRKLDEDGEEKTTITLNSRQSARIDSTLFDLHLGRPQHNIGKSLYNDLKEDILVLLDLQKYYMEKRYHCEILASQKDFLEKEIQDLNIPPEALAMQIDEENETINELIDINNNNSHHHHHHSSHSGHSHSSHGHHHISSHSSHHSSSSNTPTTTTTNTTTNTPISTPIIQATPFQMYTGQSNILEQPQTPNKTTTTTTTTTSSTPKKIAHENTSSISELINNNLTPNKKSATTGSSTLSHSSTKSTSKSSSSKKDSKSSSSSLLSPSTPSSSELSPVVPLPPTQSPVSPTKKERASKKDKKDTSAGTTTATTTAAPVTPAEPVLSKKEREKKKKEKEKEKEGKEKEKQRMLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTE2</accession>
    <name>A0A8J4PTE2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009597</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000753">
        <property type="protein sequence ID" value="KAF2069086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTE2"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="213" mass="24502" checksum="7CFEA7B54AC566FA" modified="2022-05-25" version="1" fragment="single">DIVKYTTIVKTRYPKFRNPQACQDDLNIILAEGTDEMRSIIQSCNKFIHVNNLSEDEDPDLKARKDSRTILATHLYNNCREIYKPKELDQLNDKIVNYMTEAQKSKARIDSLQQELKDTTNKNNATLAELQKIQNEIKARQESLKKAQEDAARTTAEIIRAREEAQRAREEAQRARDASNKAIDEANRARDEANRARQQAQNSGGGRRRCSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTF0</accession>
    <name>A0A8J4PTF0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000748">
        <property type="protein sequence ID" value="KAF2069101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTF0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
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        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF192">
        <property type="entry name" value="B BOX-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="404" mass="45804" checksum="BF6B765D2EEC312D" modified="2022-05-25" version="1" fragment="single">KLVAKKLKTLALELKFPRFKYQHLPRSLTSLSISVSEGQSSVPTLSLDLDTLFPNLVYLNLNQPYFQEVTGRGNLKYFCQKFTKINSIPNSLTQLKVLNLLPNDDKNINVIFPPNLTYLDYVPIRDLIKGLKVYPFSLKTLLIENDPISNTLLQDSCPPNLANYKNNHFKLTLDTPFIPPSATKASISINTEMIQLPQIPTTVTSLEMTTYYQGPFIKPIENGYFSNGITNLSLQYDLALTPGLIPSSVTNLKLNYSQPIVKDAIPTSVTKLALLGVALVQPVILPDSITCLSLLQQHNIPFITLPKNLCTLKISKNMNFQTFPYHLFPTIKTLSCNQVYNLTNFAQTFSISSGSNEYDLHSFPSNIKQVKFTYFNSTKCNFYFINQKVYKEINHERSNRFITF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTF3</accession>
    <name>A0A8J4PTF3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_005510</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000221">
        <property type="protein sequence ID" value="KAF2073192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTF3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057709">
        <property type="entry name" value="DUF7949"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25820">
        <property type="entry name" value="DUF7949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EGF-like" evidence="2 3">
        <location>
            <begin position="1054"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1001"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1001"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2073192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1104" mass="121904" checksum="DBB60DEB7D814437" modified="2022-05-25" version="1" fragment="single">MIIAPNILNFRQISMGYASASTGWNCEYTFQVTLDINYFDYIMTIPVLDFHNANPTVVNGSTLLNIGTSTSLGTYHIVGEFSKDSNTYPVTFGDDFQCIEPPPTLAYEEFPSDFNLEPQLTASRFTYFFRAKNYERRANNGAGCSLDTNLFVCHLIQPDYVSDPTLFTLVIYPLISQNIASPSTVSLTLTEPVSHSMTFTNLLEPYGSLASNVLVPPPAMVYKKDSDPNTYVLFSVQGDSTNLFLGVDQSGSGYMSYAPVKGDSQSQTYLIRYRNNPTTSSQSCNIVFYNGAAGTQHLTSIINGVPSLTIFQTYISSVQQLDPNGHTHIIQVIYIKSTYLDELFSYSLDNYFFEPSITFPLGYESGDLKEYYFSMSQMTSPFSQSQLTSISGTNSEFVTTNIGSYGVSPLAIDTLPPVINDVQTFRLNSSFVLLRINITDDVSGFSMLQFGGGLDYLVIDVLPHHLVKGDLNNGVYEIITDTLFKTPSTVAVADRASNRVMFTYDGVYNVNGKKLDLLQDILSPDTITLFYFEKPIVDVSQQGHNNTLYLNYIGAGPHSKVMFFPLFNTPDHTDVQQDPSNHYHWDPVLNLIKIDFYIPSGLVAGKLKYVISSKTGFNDEMVVQILGSNSTLTILSDSNANQLPPLVIGITAQPNSPIAITDPTNIGWVIKVETPRHRLKSALFTVTSDYDPVGFNFTFNPNNNLHINDLVINFQVLGSSRSQIYTISYAYLEDDEGLKSGFNALPWTNAFFQVEIPSITVACPILTSNPPILLSFKQISTNPINLIGANRKVVYEYEASSSRGLLARIKPVVYLTGSLNTKALLTYIRQSNFQCQTEIVSSNSTNTKYQSLCDIPFGYGYPEGLLVSVYGFVNGYMEIGGYSANSLRLKGFDYFINGSTSSFPSLPPVIDSSNNYTGSGTLELRGYRFGSSSQNLAMEIRTQGQLYTVYNPTYHHFNGFSLSFDYQSIEPFLIRFKNDNDPNYSNEYLVDPWGIGWDSSSSSATSSSSHQSSSSEIDQSSSTNITPTKEPPKCPGTPKCGGDDKGVCLSSGGCQCKYPYYGEQCTSVIVVIPTPKPNPNNPSTNITIDIPGTQDHVQLSTLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTF7</accession>
    <name>A0A8J4PTF7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SPX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_005956</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SYG1 (TC 2.A.94) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000252">
        <property type="protein sequence ID" value="KAF2072740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTF7"/>
    <dbReference type="OrthoDB" id="9970435at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004342">
        <property type="entry name" value="EXS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783:SF78">
        <property type="entry name" value="SPX AND EXS DOMAIN-CONTAINING PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783">
        <property type="entry name" value="XENOTROPIC AND POLYTROPIC RETROVIRUS RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03124">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51380">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="366"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="407"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="453"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="484"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="526"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="567"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="608"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="685"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="719"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="9">
        <location>
            <begin position="1"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="EXS" evidence="8">
        <location>
            <begin position="568"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="33"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="87"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="150"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="169"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2072740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="807" mass="93706" checksum="06E5A22C99996482" modified="2022-05-25" version="1">MKFRDHLTGNILDHWRDKYIEYEYLKSLMGEGSSTATSPLFSPPLRSTPSVVDADGDSIIPIPVGRTSDIDGDSNGGADTSAQDRLSTNSIHSSNSRNSINSSSSSSSSSSSNNINSSTEQMLSSEEKHADSSSSSSLTEDEGTPKPNRHNPISPKHNNNSNHHHHQSNKKDNPDTEIEMKVRKPKLKKSISIKIEKFLDNRKSKSNDKQCYEGETFQEGFLAQLDKVNHFFIDRFTKFKKKTVELCNMIQFLSSNSELRTNRNINYVRQQFIYNSQNLTILLNYRKINMEGFEKLLKKLETKNQILSIHLNTIYQNKSVVTDDCSSVKHADQVKQIFSRFFTGNNQKMANSQLNQNIDKEKDGKWLIFQIGLLMGLSIVLGSLVIYNYRFYYPHDSPPPDSALGWLLFRITLLPILLGTSFSIMSLFWERAGINYEFIFELKPDASRSSIKYFRGGMVFIFFWLLCLYFYIDTSSYIQKIPPISFPILFMLVSLFVGIMPFPIFEMSTRFWILKRIGKVISAPFVPVRFSDFFMSVQLLSLGEFLFNIQSMVCIFNKSGLDPSEAHFCTQSAFFAFPLLNALPYYWRVMQCFRRYYETRRFFPHMTSAIRSIISIIVLILSYIALQTSETNNWNIIKLIWFNFNILGSFYKWYADMAVDWGFLLNFKTNKAWPLREKLLYKKKIIYYIAMILDLFLRFLWLLVFAIRKGTLHRLDNPVFLFFFSMGEVIWASQFIYFRVESEHCLAADHYSTFYDIPIPFSEQYNLYIAEKQKQSLQKQKLTQKDSKASLEKQDSVELNDLLNIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTK6</accession>
    <name>A0A8J4PTK6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009491</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000722">
        <property type="protein sequence ID" value="KAF2069191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTK6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="80" mass="8713" checksum="E6D5BA79739896B9" modified="2022-05-25" version="1">MYDTLIKNKTLECFTAFEIFKCATPLVPLMSTNCGLYRGEHTKLETLTLSKTLSACSESTYTVPSGTGKSSTPISDDLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUG0</accession>
    <name>A0A8J4PUG0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Myb domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_004864</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000180">
        <property type="protein sequence ID" value="KAF2073837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUG0"/>
    <dbReference type="OrthoDB" id="118550at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SANT" evidence="2">
        <location>
            <begin position="726"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="731"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="619"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="777"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="820"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="155"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="214"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="254"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="619"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="658"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="669"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="687"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="716"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="820"/>
            <end position="841"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2073837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="860" mass="94677" checksum="E3822F500F465681" modified="2022-05-25" version="1">MQQSPPLLNNTGGIVSPPVTSVPPQQQQQQQPYGVVQQQQQEQGAGGILNNNNNNNSNNAGQSQPQIPQQQQGVALQSNVGGVPSVNSAGGVPTTLTNSQIIQKLKHNQILIKTILEKQNLGRLNECLSYQNLLHSNLLELVPYANRQEQFNQHMQKQMLAQQQQQQQQQPSTGIKSQQAPNVVSPQVNATSTPPPQPQQPTAGTSSLGSPKGNQPSTPSQPNTPQVSTSNDSQQQQTSTPQQQWQQPQPPQPNQQQQPQTSPNTNMYSPANPTPQQQQPQPSPVSYSSPQTQTTSPAQPNQMASSIQTNTPQNQQGQQFGTSQQPLSNLSPNTPSNTIQQQQQMLYQVYNNLRQTLAQYQNQGQTPPPEITQQLYSVTQQIQQIQAQQQQQQQQQQQNTPTQVTSPPTLQQPQQQDHSALQMQPNQQQSQPQPQPNQQMQPNQTPQQPNQVYQQQPQQPQMPNQTQQFNQSQTVDQNMQMQILNQRFESINTLFSQLMGISNSMVTLNQNLVNATDDQTKQGINQQIAIYRSQYSAIELSIVNSLRALPPNYQQMILQMQKQHQQQQQSNQMFQFKNQPQFVALSPQQQQSFLQSLQQQHQFQQMQLQQQHQSIVNSLQQLQQQTPQQQPTSNLQSMGVISPINPMGQMSGMDGALQPQQQQQQQPPSTTSIHATPSSPPAINNVPSTPTATSTTAPPTSSSSSSSSKKEKSSKSKSSSSSSSSSTTSHWSAEEHQKFLEAVAKYGIKDHHSISNHVQSRTPQQVRTHVITYQKKLKKEAGSGGGSTPSQPLPPPTPTTISNSTDNLSINTSGSISLAASTPSLNPADQNFSSPLNNTLTPSSPPISQSSPELGAHLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUT2</accession>
    <name>A0A8J4PUT2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_004312</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the XPO2/CSE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000150">
        <property type="protein sequence ID" value="KAF2074378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUT2"/>
    <dbReference type="OrthoDB" id="3268246at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000507">
        <property type="entry name" value="Exportin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005043">
        <property type="entry name" value="XPO2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013713">
        <property type="entry name" value="XPO2_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF8">
        <property type="entry name" value="EXPORTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03378">
        <property type="entry name" value="CAS_CSE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08506">
        <property type="entry name" value="Cse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="8">
        <location>
            <begin position="29"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2074378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="936" mass="107467" checksum="AF5621A3D1504660" modified="2022-05-25" version="1">MELTEQVLNKLVICFTNSNNPTQSIREEATNEIKNISKTPGFLMVLLKMLDIPSVDLVVKQMAAICFKNVAKESWLETDDTENPIPIQDKNSVKEVIVNVILAQTNNPLVQNQLLAAMTIIGDIDFPQNWPNLLPELVQVMSDPNVDFQKLPPILKIMHSILKKYRNSSNSQEVLKELQYILTILPEPFLNLFIKTGQQINVAAQNEKELFILFTSIHYLLEIFHSMSCVDLPEFFEDNLEKFTTEFHRYLQLNIQTPSIVQSKSEEEPSLLNNIHTVICDIINLYTQKYDEEFQNHLQSFVQDVWGLLSKTNSEPRNDHFIYSSVKFLSTVSMSIKHDLFNSEDILRQICSSIVLPNIQLREGDIELYEDNPVEYMRKDIEGSDSDTRRRASIDLVRGLRKYFESVITRLLMADIQKLLQQHQSNPAEYWMAKDSAIFLVTALAVRAENQSANQLVPIMDFYKSQIEPELLNPQCQPILRADCLKFISIFRTQIPAQEYTRLLQHILPCLESPDYIIHTYAATCIDRLLTVKDNGVARISPDFVSSHLSGLLLPLVNVFNFPCSKQNERAMRTIVRIVLMTLGKIDLVTTTQLLQKFVNILLEEAKNPLNQTFNHYCFEVVGSMLKSYANIPEVIHIVIPMIEYILQKDVVDFSPYNFQLLAILVENSPPEFMDPYRSMVPIICHPNLWKRHSNIPPLVRLFQAYFKKDAMSLVANKHLEPILGIFQNLLSSPANDHEGFYLLEAIVENLPLDTYKQYIVRVLQIILERISKQKTSKVVRCFIIFYSLFIIKVGVEQVCQITKTVRPDLWEVILKKLFIPTTTEIKGKIEKKIISIAITNLLSNAELFAAAKDVWVALCEAQLNIFKGKETESAHADDELYIDKNENVEGYTPTFSQLQFSKKQDSDPFPQIDPKSYFDSNSTIKSLAQQANVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PV28</accession>
    <name>A0A8J4PV28_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004947</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000185">
        <property type="protein sequence ID" value="KAF2073745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PV28"/>
    <dbReference type="OrthoDB" id="1918at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014848">
        <property type="entry name" value="Rgp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12507">
        <property type="entry name" value="REDUCED GROWTH PHENOTYPE 1 RGP1, YEAST -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08737">
        <property type="entry name" value="Rgp1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="467"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="98"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="468"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="479"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="567"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="589"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="609"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="682" mass="75172" checksum="76CCEDB7DFAA21D2" modified="2022-05-25" version="1">MTIVVECILESAVFFPGDTVFCRIYISNKSLLPYHHSHHNHHQQHSSSSGNSNKEREQNSISWITAQLYGNLMVESSYIQLSSNRGNKKKAKEKNKDNNNSNSSSNTPSKTSPNTNTSTHTINNNNNNNNSSSSSSSSSSSSSNITTKSLINANSLKKYTAGGVSSATSLPNMNEIGGENGKTIFASPTTFLASDLYIPPGQTRSYIYYVTLPSPLPPSFKGTSLKYSYFLSIAVQVYTNPAQILTVPIRVLTPASALGIIKFKSNMSFHFEEGSAETTQENQKSSLEVISKWPVSPFKKSQYFPVPYPQTLSQYDSNNTRKSVIDILNKQSTPMTTIISKGTDKLAIFSICKTAFNLGDSVYGTFDFSIAMIPCYKILVKLECEEVVDPKYLQHSRGSHSKPIRKLFGELHEYTTNIRQTHFTFHIPVEASQEFSTKYVSVKWSLRFEFVTPLKSSSSFLPYQAPVAPQPSLPPQQPPVTITTTTSSSSSSSSQPNTPPITPNKSVSVEDLTNLRHLSPRPSIPKNSSFSAEDQQQHQQHHHHHHHHSDDSDSSRSENANIDDETMSNSSSTFSTSTGRLHGSSSSSIQMVTATGISSSGNLPPPPNQQQLPQPQPQQQQTSLQTQPTRKRMSANPNIGILKDDGVSVPLVDTLHWSLPIRVLVSAPPPELLHTNKNELHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVC5</accession>
    <name>A0A8J4PVC5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_004472</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IFI6/IFI27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000158">
        <property type="protein sequence ID" value="KAF2074226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVC5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009311">
        <property type="entry name" value="IFI6/IFI27-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038213">
        <property type="entry name" value="IFI6/IFI27-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16932">
        <property type="entry name" value="INTERFERON ALPHA-INDUCIBLE PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16932:SF18">
        <property type="entry name" value="INTERFERON, ALPHA-INDUCIBLE PROTEIN 27-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06140">
        <property type="entry name" value="Ifi-6-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2074226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="146" mass="15390" checksum="E9C51ECB46C80375" modified="2022-05-25" version="1">MQEHETKSKSKLEDAKNLVKNVVDKCKQNPNYTGTATGLSASFGTIIVVPSLLQGIGFTTTGILSGSWAASAMAYLPVSLVATGQSIGALGTIFSAVSGTPIIISILAFGGAFGLATKLKIDRVNKKEQENKLNNQFSRKTIKSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVL2</accession>
    <name>A0A8J4PVL2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ras-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_004666</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000169">
        <property type="protein sequence ID" value="KAF2074015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVL2"/>
    <dbReference type="OrthoDB" id="1562946at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF151">
        <property type="entry name" value="NEUROFIBROMIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GAP" evidence="3">
        <location>
            <begin position="56"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="312"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="435"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="318"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="353"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2074015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="844" mass="95078" checksum="3C734561DAA1DAED" modified="2022-05-25" version="1">MSEISTNQNNLNDKHNLQWTKNAWLNSSHLYGELTSICIKDTVFTRSFANIIDNSEVDAFSKNIVNIFSAANKTLPLIKDLIYNEFNSKVSAEGEGSILRGNSIVNKIEGAYVRLIGANYLRSVLSDLVTKVVSDKDLKLEIDPRKLNTYDDEVVVGYDKKDTDTQIKENQAALYETSQMFLDRITDPAVIEEMPREIRAIADYTAESALKYAPDSLAPLVGGFIMLRFFSPAIVTPEYSKLLSSDIVPSKRGKRNLVLLAKVLQNASNGVMFGGKEDFMTCMNSFITANRSKMSNYFTLICKDPLHPDNKWSDLEGPSSPKSNSSTTTAPSTTWSNSTPSAKPASKWLATTPSSSLPVSSPSQSSSSSATNKKGSSLTVSTKTLEGYLKNIEMNDLFDIHRIFDLYKDKITLKLVPTSQSALRVIEILKELGPSPKTKTEKKKKETDASEEPSANGASTGSAFDECTHMLERARFLFQGPNDKNDRSVFYLIVNRVKPEVFDNINPLIAHIFKVMDVCANSSYTLVVDMSWAHISNELKKSIFTYLPNLAEIFSRKAVVYFMRAFTSRKLKRKIHEVYNWKELTSFIDSENIALPETSKDFITKSYRVVKVNSKGKRQERLIKFTSNSLLNIDPKTHRIQNEKRIDEIEEISSRLGSIEISMKLSSSADPCTFSKNTSLTGRIGFLSLKNNNEGSTSRKYVCNHELERDHILQDIFETGFKMGLRKSSKNLPTEYKVIKVNGAGKHQERIFKLTIDSLLNLDQQRIKSENSFAGIEEVVLDTEVEDVVWIKYKSERHKRKIICSKGEGKLLWEVLTDAIHKYQTSIDIQEGAFKLDAEEAAYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVQ1</accession>
    <name>A0A8J4PVQ1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of DNA damage checkpoint protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_002809</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000082">
        <property type="protein sequence ID" value="KAF2075873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVQ1"/>
    <dbReference type="OrthoDB" id="342264at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051579">
        <property type="entry name" value="DDR_Transcriptional_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23196">
        <property type="entry name" value="PAX TRANSCRIPTION ACTIVATION DOMAIN INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23196:SF1">
        <property type="entry name" value="PAX-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16770">
        <property type="entry name" value="RTT107_BRCT_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="FHA" evidence="13">
        <location>
            <begin position="41"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="14">
        <location>
            <begin position="553"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="14">
        <location>
            <begin position="637"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="127"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="127"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="156"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="217"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="253"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="455"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="480"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="505"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2075873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="733" mass="84228" checksum="8549A1A5FCC4F532" modified="2022-05-25" version="1">MNNTASDQINNKHKVMHPTLKAKLSHSTLPFDEFPIVKGVNGIGRASTNTIFFQREQTVSNYHAAIEYDGERVFLQDLNSLNKTQLLLRPGAEQIFLIPNKKYELVNGSKIIFGSVPSQIIIYNSPKPQSSPSLESHSNTTSSVDTATKVTPVDQNSSIDNNSNSNNNNSNSNSNSSNNNTHTSESTSNVNINSNNDDFVSEKEKQDEEKQEDNEQSLDKPKESHSDNEQKDIQQQQEDKESQQKQGSQNQFIDKEQLDIIEQQHREREEIERQEKERQEKELKVKEDQIKKQREEQEREVNERLQREEKEKQEKERQEKDKRDKEEQIKKQKEQEEEKRVAEQVRKEKEENEKRLEQEQIKKQKEEEEEAKRIEKEKVNQQLCLEKERIALEKKQQQEQKEKEEEAKREQEKKEESEREKQKQQEEKHQENDIESSPAPTTGKKATKNKKEEQQQVSQEQSDSSQSSNKTPRKKRQSALIEQTETLTPTSSKRKSIKLGLLDDTSSSGIVGSGSSQTSQSTPSPMNIDNNRSPSILFSLIPEEFIKQTTKDIVGLGGVMAESVRDCTHLICDELKRSKKVLESIALGKIIVTSQWIQDSKKAGHFVDETPFHLLDKKAEKEWSFNLEKSLSNARSHPAQLYKGTSFFITANSIPPKDFLEEMITHAGGKVLDSLTKVVVTPDTKVVVLCSPDKDKKNFKKWKDANYYLSKGDFILLSILSQTFDLDNNSSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVT8</accession>
    <name>A0A8J4PVT8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005019</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000190">
        <property type="protein sequence ID" value="KAF2073669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVT8"/>
    <dbReference type="OrthoDB" id="13613at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34003">
        <property type="entry name" value="BLL2395 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34003:SF2">
        <property type="entry name" value="SNOAL-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="116" mass="13390" checksum="0E0901ADAA3BBB0A" modified="2022-05-25" version="1">MSSNNIKTNLESIFEGIRQNKIVETFEKYYDEKVVMFEKGDSTNRVGKDQNRVAEQGFQSNATIHELKIIKILIDGNNSAYESEMTFTYGGHKVTKTQWAIQEWTPSGLIVKEEFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW13</accession>
    <name>A0A8J4PW13_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">sn-1-specific diacylglycerol lipase</fullName>
            <ecNumber evidence="14">3.1.1.116</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_003940</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1,2-diacyl-sn-glycerol + H2O = a 2-acylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33275"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17389"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.116"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:33276"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.116" evidence="14"/>
    <dbReference type="EMBL" id="AJWJ01000131">
        <property type="protein sequence ID" value="KAF2074752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW13"/>
    <dbReference type="OrthoDB" id="438440at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019369">
        <property type="term" value="P:arachidonate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046340">
        <property type="term" value="P:diacylglycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000321">
        <property type="entry name" value="Lipase domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052214">
        <property type="entry name" value="DAG_Lipase-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45792">
        <property type="entry name" value="DIACYLGLYCEROL LIPASE HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45792:SF10">
        <property type="entry name" value="FUNGAL LIPASE-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fungal lipase-type" evidence="16">
        <location>
            <begin position="502"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="63"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="181"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="66"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="181"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="198"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00026104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2074752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="803" mass="91628" checksum="4E692E35D311316D" modified="2022-05-25" version="1">MTNIKEKDETKSVGEKEECNQKNQQGELYQDTKHDIDFHIEESENEWKLLPKEEFHTLSTNSLVCSSNSSSNNNSSDSDSNNNIVSSNNNNNNNDNEKMTNKITTTTSHFKSGDIESVFVGNIETTSLDYKLMEVDPNQYKLNDKTQDSEKEKIKEGDFKILLDLSTDQHKDIQDSFEIYNTSNNLPIPNISSNTPDYIRDSRKEDSDNTKEKEKSKEEKEKEKEREDKQEKKDFNDMPLKFKGEEANLLFRSHESIDPPPQSPTPFPRKVSGLKKEEDFFLSSSSSSSDSEIADGSLEEQLQKYVVQEADDSLVACSLSEKQIIKKKFLKKKLKKEQFERFYLDDTAQIQPSPELVQQSKSLSELGKKSMTVITDLLDRLFLHKRYSKIDLFMGLALLNSYYKEIIIRNWTCEKDKGFLEEALRYQRFSTAAYGRKLYYGMMNSNIINFFKGLAGTDNTNLKVIIKHTGIKKKDVIVSKWFSSKYSPGHYFAVDHKTKSLVFVIRGTFNHFDIITDLVAKSYIYKDGMAHMGILLCAHMKMREMYPLIMKSLDVYKGYRLVVTGHSLGAGVASLFTILFNEVQPHIPIRCFSFGAPCVLSLELANHLKTKSLISTFCMNDDIIPRLSFNSLFYLREVLDSILSQSSTKIQKIFQIVSANGLNEKMTRRFSKLLKVAPTINLTNVQHSPSGELPMYPPGQLYRIVKLSKGIYVAEKTDMESYSKILVSATMFTDHMPQKYEKGLSSALENFNKPEYTEKRPEDVIEEQFLGMEGQASASSTSNVSVIDLNEVIPETLGASIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW15</accession>
    <name>A0A8J4PW15_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_008091</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000471">
        <property type="protein sequence ID" value="KAF2070586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057709">
        <property type="entry name" value="DUF7949"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25820">
        <property type="entry name" value="DUF7949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035307528" description="EGF-like domain-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1341"/>
            <end position="1364"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="1077"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1020"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1026"/>
            <end position="1050"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS00022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2070586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1384" mass="154127" checksum="9DAFAFE7C2E3A2AA" modified="2022-05-25" version="1">MIKLNLVLPLFLIVLLLVIENDAQNIAEIKKVSFSDNSYLNGDQICIMKSYIVLDSDTPDKITIKSSGVMVVYSLLYSITEKYDQNLYKTLYVGDFETQQGVNSISIFLEFANGSISDLLSLGTNTCWPIPSAHATPTYPNTLFPKRLNFYFLRIPSIVSTWYNGYILFTMNPGSPYVGNIRQSPYDTSFFIVQLYLDTLATPPVEANFTLQFSSSEPVNYSVSSSMRAWDDIIGEYNISMKLENVYTKAQPFCSFMIGVQGDPRQVYFEQPALSPSGSLFREYVKGNPMTSSLLVNTYVISINSMLPNIPLPISIIAYNGIAGPLELETVSVNHYAPSLITQLPIIDTELGALPGGTRYIVCKTSIQTDAMTQLEIKITNSKGTIADYPFGFKSGNLKNVDIVVSYVLPPYFSAYNPQFSVEYPILTSSAPTPFIPIQIDSTPPIIHSIDITPLDKGKYLLLADISDDLSGFHSIEFNGKVILQASNIIQGTMISGLYEAIIDQNDLLFINQIKVVDFGFSFSSFPDGYYNSKADRVQLIGSLFIPSNIKLFKFEYYNLDLSISGAWNTLYFNYEGAGPYSKIIFYPIIVVSEHSVIDNPDIYTDPQYQYEWDIESKSFKIRFYLPAGLYSKNINYMVGDLSGTRLYAFDDFYNLIGSDAVLNVSSIDANQMFPYVTEFEIIGTSVIYLNGPTQTANIGWRITIETLKHPLKYATFMVTSDYNPQGVNYTFVPSQSMNKSPFNGVYEILFQVDGDSISQEYQISFVLFEDKVGRKSGINNPKIGDAFLKFHNTSFYPIKVNCLPISDTTSPVLTGWAVSTQTFNSMSGVNVFSIDFETQDVDSPLSLDHIPVVYLTPPISQSKYQCKTRLLGSTPGNKTATYRAHCIPPYGFGFPLGFSFSIYGIVDKYMNVGGYSSSDLLNDNRQHYMNAPTPSFSTPVLIQGQSDGGNVTLTGHHFGVANESVQIFIYPDNADTIQISAPLFKYFSNLQFSFIFTSQTPFALSIQVKNIGSNTLRIIPKNKTDSSSTNSENTGSSMEPSSSSTSSHSIPKNIPCPGNPVCGGKDKGICLPNGGCKCLASYYGEDCTSVIIIIPKPDFKPNQPTTNITIDNNDIALESLISIVGLNELDQTGQIIKRMTFDQWIYTKINDDLHQYNTSIVGAIQTNISVWIEYFEEQQIIQFAGQNLTMYPSSIKYKIAITRYPFSSSLNMLQFVMAASFITPTNGECTYTDFNNTIEDSEYLRLQIDSHSLFGRFIKRGLLDDRPRVVTNSILENDGYTSFKNTSMGSSFIGMNLPFYLRTAIIDPDFSVLIDSNPASSKQGSTCATKKTNKLSGAKIAGIVIGCVAFVSIIVVAVSYHLYKKKKQLKFEKNVENKLKRFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW81</accession>
    <name>A0A8J4PW81_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Profilin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_005538</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the profilin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000223">
        <property type="protein sequence ID" value="KAF2073149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW81"/>
    <dbReference type="OrthoDB" id="421374at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003785">
        <property type="term" value="F:actin monomer binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048278">
        <property type="entry name" value="PFN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005455">
        <property type="entry name" value="PFN_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036140">
        <property type="entry name" value="PFN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11604">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11604:SF0">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00235">
        <property type="entry name" value="Profilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00392">
        <property type="entry name" value="PROF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55770">
        <property type="entry name" value="Profilin (actin-binding protein)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2073149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="124" mass="13215" checksum="1F46497D5814D397" modified="2022-05-25" version="1">MATNWQTHVDSLVGSGQVRGACILNVKGFLVAKSNNINLHDGEGDKLIKVFTEPKTKGVTIHGVTYAIFKTDARSIYGKKGTNGCIVLKTSKSIIVGVYDSTIPQEKATLAVEGLAARLIKDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXC2</accession>
    <name>A0A8J4PXC2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002094</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000055">
        <property type="protein sequence ID" value="KAF2076608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXC2"/>
    <dbReference type="OrthoDB" id="197432at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052724">
        <property type="entry name" value="GT117_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021280">
        <property type="entry name" value="TMEM260-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16214">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 260"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16214:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 260"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11028">
        <property type="entry name" value="TMEM260-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="177"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="232"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="270"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="393"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="457"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="491"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="807" mass="92433" checksum="429ECFC817596A8F" modified="2022-05-25" version="1">MKNKSSTNNNSNNNNTSTTSNNVNSSQTKRRNNSNVIQNENLSNSSSNRNNSQQQQQQQQPRITQNTNVNWAKDFMNWNDQQQQQLQSVGSTVVVHQKDKYFKVVKRSTVIISCLIFVITTLLYSFTQYPSVSGGDAGELIINAYQFGIAHPPGYPLFTALGYIFSHAFPSSQLSVAWKVSLMSSMIGAIGSVLIYLTVYLWVNDHWSGILASGLFTFSPLIWMYHIQGEVFSLNNTFTALLLFLSIWFTRVRIYENERYNASFWTSERIAYLSAFLCGLGLTNQHTLVLIVAPFAFWIMFIAGRDQLWSMKIVSNLSICALSGLSPYLFLFVAPKLNKVKYSWGNTSTISGFITHFLRSEYGTLQLYSGDEGNSISLLPKIFLYFENLVDQFTIIGVILALIGFLNIMFGYNLKTFKWKSIGTMIGFTFLFYITFFFNLCNLPIDKSLYKGVFLRFFMQPNVIVSICIGIGSKSLFRFANNSNNNILKKILLPLLVLGLVGYQVSTNYSLQDQSKNYSFHDFGYAILDSLPKNALLLAGGDLVTNVPQYLHLCEKARPDIDIISLEIMSWDWFTVTQGPLYSRVNFPGSVYHPYKTDGYSLKLFLDYNQHRPIFIVGDFKHGDNSFQSHYFTVNRGMAQQIFHVSQKQDLNLYQMIRQSFSKMPEFNNLPNDTVKYPADSWEHHMINDIVSNLEKSVENLLSHYLSLETVDSDKALELSIDILTKALELNPTRCWALKHMGVTYDHLRYRLMKPTSNSADSKHTADKAESYSKSLLTHWSKYIEHCFKERDQDWDTINNVVLYYNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXI4</accession>
    <name>A0A8J4PXI4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 11">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="1 11">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_004106</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="11">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="1 11"/>
    <dbReference type="EMBL" id="AJWJ01000139">
        <property type="protein sequence ID" value="KAF2074599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXI4"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07852">
        <property type="entry name" value="STKc_MAPK15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000238">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000166">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="15"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="350"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="363"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2074599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="374" mass="42410" checksum="322A3C209042DF9A" modified="2022-05-25" version="1">MTSSEDIDKHVLRKYEVSQKLGKGAYGIVWKSIDKKTKETVALKKIFDAFQNATDAQRTFREIMFLQELHGHENIIKLLNVLKADNDRDIYLVFEYMETDLHAVIRANILEDIHKQYTIYQLLKALKYMHSGNVLHRDIKPSNLLLNSECLVKVADFGLARSITSLESMTEANPVLTEYVATRWYRAPEILLGSTKYTKGVDMWSIGCILGELLGGKAMFPGNSTMNQLDLIIEVTGRPSAEDIEAIKSPFAGTMLESLPPSNPRSLSDMYPLASPEALDLLRKLLQFNPDKRITAEEALAHPFVAQFHNPNEEPACDRIIKIPIDDSQKLPITEYRNKLYNDIIKKKKEARRKTLGQKPESDSNNSTPNTESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXX9</accession>
    <name>A0A8J4PXX9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar import and degradation protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004542</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GID4/VID24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000163">
        <property type="protein sequence ID" value="KAF2074139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXX9"/>
    <dbReference type="OrthoDB" id="62at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034657">
        <property type="term" value="C:GID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045721">
        <property type="term" value="P:negative regulation of gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007039">
        <property type="term" value="P:protein catabolic process in the vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018618">
        <property type="entry name" value="GID4/10-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14534:SF3">
        <property type="entry name" value="GID COMPLEX SUBUNIT 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14534">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09783">
        <property type="entry name" value="Vac_ImportDeg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00061469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="230" mass="27123" checksum="934D8B2B9F77A13C" modified="2022-05-25" version="1">MKFRLVLKNIHIKLAVEQIVSEKNTCNNNNGKNNSEDLDIDKNKTTSIPKFALYPGSRFVGVQKSERTSYPVLVDIKNVDFKNSVLSGYLTISGLTDKYPVLTTFFEAEIIGDKYPFLTRKWDADENVDKEHWSQFPAFGAYHNKFNRDDFKVDFDSSDYLFMRWKEYFLVPDHRVKTIEGASYEGFYYISYQRSTGIIKGYYYHFSSEWYQSLELNHQPQSTFPVYEFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PY49</accession>
    <name>A0A8J4PY49_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein phosphatase 2A scaffold subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_003631</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphatase 2A regulatory subunit A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000116">
        <property type="protein sequence ID" value="KAF2075064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PY49"/>
    <dbReference type="OrthoDB" id="340346at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000011">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A regulatory subunit A alpha isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055231">
        <property type="entry name" value="2AA_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000357">
        <property type="entry name" value="HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054573">
        <property type="entry name" value="PP2A/SF3B1-like_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051023">
        <property type="entry name" value="PP2A_Regulatory_Subunit_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648:SF4">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2 (FORMERLY 2A), REGULATORY SUBUNIT A, BETA ISOFORM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A 65 KDA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02985">
        <property type="entry name" value="HEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22646">
        <property type="entry name" value="PPP2R1A-like_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22956">
        <property type="entry name" value="VPS15-like_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="12"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="90"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="167"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="206"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="245"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatase PP2A regulatory subunit A/Splicing factor 3B subunit 1-like HEAT repeat" evidence="4">
        <location>
            <begin position="277"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="284"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="323"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatase 2A Regulatory Subunit A helical" evidence="5">
        <location>
            <begin position="359"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="362"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="401"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="518"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2075064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="583" mass="65327" checksum="BFB15EBA7B31EB84" modified="2022-05-25" version="1">MASMNESDDCHPIVILIDELKNEDMQIRLNSIRKLQSIAKALGPDRTRTELIPYLQDSVLEDEDEVLVALSEELGKLTDFVGGPEHSVFLLPPLQILAGAEELVVRDKAVESLNKIAAEIPNSAFEESFLPLLFTLSKADWFTSRISACGLFTVSYPRSNPEMKKSLRKTFTGLCHDDTPMVRRAAATNLGNFSKQIEKESIKTEILPLFQSLSTDEQDSVRLLGVENCALLGSMLTLEENINLILPTIKSSSQDKSWRVRYMVARLFKELCDSMGPEITKTELVQAFVKLLKDTEAEVRTEATLRIADVCSLLPKEMNIKNILPCIKDLVTDSSQHVRAALAQVIMSLAPIFGKEDTLEHLLELFLQLLKDDFPDVRLNIISKLDQVSKVIGIEMLSQSLLPAIVELAEDHQWRVRLAIIDYIPLLASQLGVEFFDEKLGNLCMTWLGDPVFSIREAATNNLKKLTEVFGVDWAKNNIIPKVLSLHTHPNYLYRMTTLFAISVLSTVVGSDVIASSMVPLLAKLVTDKVPNIRFNVAKTFQTVIPLLDSTVVQSRVKPLLIKLQEDYDKDVKFYATQALQLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZX2</accession>
    <name>A0A8J4PZX2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial</fullName>
            <ecNumber evidence="11">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ATP-specific succinyl-CoA synthetase subunit beta</fullName>
            <shortName evidence="11">A-SCS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Succinyl-CoA synthetase beta-A chain</fullName>
            <shortName evidence="11">SCS-betaA</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_002601</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>succinate + ATP + CoA = succinyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterodimer of an alpha and a beta subunit. The beta subunit determines specificity for ATP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterodimer of an alpha and a beta subunit. The beta subunit determines specificity for GTP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the succinate/malate CoA ligase beta subunit family. ATP-specific subunit beta subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="11"/>
    <dbReference type="EMBL" id="AJWJ01000073">
        <property type="protein sequence ID" value="KAF2076087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZX2"/>
    <dbReference type="OrthoDB" id="1552at2759"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042709">
        <property type="term" value="C:succinate-CoA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006104">
        <property type="term" value="P:succinyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000002">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000001">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000004">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00558">
        <property type="entry name" value="Succ_CoA_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03220">
        <property type="entry name" value="Succ_CoA_betaA_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013650">
        <property type="entry name" value="ATP-grasp_succ-CoA_synth-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034723">
        <property type="entry name" value="Succ_CoA_betaA_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005809">
        <property type="entry name" value="Succ_CoA_ligase-like_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001913">
        <property type="entry name" value="PRK00696.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01016">
        <property type="entry name" value="sucCoAbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815:SF1">
        <property type="entry name" value="SUCCINATE--COA LIGASE [ADP-FORMING] SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815">
        <property type="entry name" value="SUCCINYL-COA SYNTHETASE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08442">
        <property type="entry name" value="ATP-grasp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001554">
        <property type="entry name" value="SucCS_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2 11" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="ATP-grasp" evidence="14">
        <location>
            <begin position="38"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="82"/>
            <end position="84"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="358"/>
            <end position="360"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="11">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="11">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2076087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="441" mass="48099" checksum="667009443F81747A" modified="2022-05-25" version="1">MFTNLVKAGSRLNKTNNNANASLVYTKRFLNIHEYQAQRMMKGFGIDVPKGNVAETPEEAEKIAEVLGGQDLVIKAQVLAGGRGKGIFTTSKLKGGVHLCSSPTQIKDLATKMLGQTLVTKQTGPEGKIVHQVYIAERHFLRKEMYFAILMDRKAGGPVMVASPEGGMDIEAVARDNPSAIYKEPIDIMTGIRPEQTKRLAEKLGFSPKNIAKAQDQMAKLYQLFLKNDCTLVEINPLAETATGEVMCMDAKLNFDDNAQFRHKEIFDLRDKSQEDAREVKASEYDLNYIGLDGNIGCLVNGAGLAMATMDIIKHYGGNPANFLDVGGGATQKQVTEAIKLISSDPKVKSILVNIFGGIMKCDIIALGIIAAVRELSIKTPLVVRLQGTNVEYAKKIMEESGLRIIAADNLDEAAQKSVRIAEIVALAEQAELDVSFKLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q023</accession>
    <name>A0A8J4Q023_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001321</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000030">
        <property type="protein sequence ID" value="KAF2077393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q023"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="507" mass="58279" checksum="53BF1A7E783F48EC" modified="2022-05-25" version="1">MSTVYIPLKQINDTTQPTHQDNLNIHHFILKSFMFYKKKNPFSNIILNFKKKAIHTFYYISNDEHKTVFKIEKLSTSGSLTGEEEEKLKNCLTKDISTLTAEEQSLDKIIEAIKVKGDYIIHYTDKANIKEDQGNESFLLIENLDHTKVSLSNIVKTKKEERDFEFTIEQEEAGYPHDLILRVRVTFKNKPKFFELKIIEGEYYLGYTKQLEKPTQEVEEFMVKLVVKNSDFTNPTDSIQVKIISDEFKDSFFFNLHTEYRFNDIIDSFGDCKAVLSIHGGPRTGKSTLGNNIINFMISENKTHLHVGGNMDASVTTEGEYVSCNKLAEIKKSTESFPYADLLLKMFLTVLDNPPITKSNTTANDGNFEKTREIIEANITLQIDGKYPNKKEIVFEANNENKVDVFIFLFPVQDFISCQDKVDFIRLFTIGIEVVTLKHKSFPIVALTQYENVNPQIMNQALSAIAFPVLKSNIFKIPKNPSKDTLFRLIRKVFEIKRAKTGKIISA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q065</accession>
    <name>A0A8J4Q065_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7743 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001426</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000033">
        <property type="protein sequence ID" value="KAF2077301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q065"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035226320" description="DUF7743 domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="2">
        <location>
            <begin position="446"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="536" mass="60152" checksum="DC126DE147212294" modified="2022-05-25" version="1" fragment="single">MLCYNQFVLFFVLVLGLLVGQSVGTIVLQDLSDVDFNRPYGTYDGADPTKVNCFKIFTYLITDPQATSEITLIDVSPNITPEPLLRNFTTLLFQVKFSYTNQEPSNFKFNVTSGSQNERYEFNSPCLGEPTTFEIELYNQTYPSIRYYLDLTKTDTTFDFTFKAKGFTNPQYSPNLDITCNNTCYYPSVTQPAPNIYTVYLTYMCRLSDIDPNDNILISVGVYNTQPFTNTFILNSYLKASKTPMASIGLNSVQLYPLGLDNSSFYDQRHCYSLIQNNGFDLPILTQLSNGGYLTSGNLVMRSGSTFVFLYPNWEQIGPNQLQLSAYAGGNSLVNDSISFSYFGPTNRPIPGVINPLLESEFEILSFTADSNCTWQCGYLYYVYGVNNGISTWLSGFWPYGATRGHSLSHNYTVDMFISPYIAKTVIVGLIGTSKSINKVLNSAPSDAVAPLVTSLKIISIPNSPFDIVRMTITDDLSGFSKLLGLGDYRNLVQGSVLNGEYDFLVQTNQYYPFNYGFKTIYDFASNYNQFALNSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q071</accession>
    <name>A0A8J4Q071_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_001992</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in pre-mRNA splicing and cell cycle progression. Required for the spliceosome assembly and initiation of the DNA replication.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the crooked-neck family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000051">
        <property type="protein sequence ID" value="KAF2076735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q071"/>
    <dbReference type="OrthoDB" id="541719at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000048">
        <property type="entry name" value="Cell cycle control protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000306">
        <property type="entry name" value="Cell cycle control protein cwf4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000796">
        <property type="entry name" value="Crooked neck pre-mRNA splicing factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059164">
        <property type="entry name" value="HAT_PRP39_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055433">
        <property type="entry name" value="HAT_Syf1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055430">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045075">
        <property type="entry name" value="Syf1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246:SF3">
        <property type="entry name" value="CROOKED NECK-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23241">
        <property type="entry name" value="HAT_PRP39_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23231">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23233">
        <property type="entry name" value="HAT_Syf1_CNRKL1_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1-like N-terminal HAT-repeats" evidence="10">
        <location>
            <begin position="60"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1/CRNKL1-like C-terminal HAT-repeats" evidence="9">
        <location>
            <begin position="217"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1-like N-terminal HAT-repeats" evidence="10">
        <location>
            <begin position="309"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2076735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="666" mass="79722" checksum="D5FC4A1C4FC2D834" modified="2022-05-25" version="1">MFNPPKVSKVKNKSAAPVQITAEQILRVALEQQQTLPKSAPKQTIADIDELEDYRTRKRQAFEVSLGRNRGVTSVWMKYAQWEESQKDLTRARSIFERAIDSNYRNEVFWIRYAEMEMKNKNINLARNVWDRAVSLLPRVPQLWYKFVFMEDMLGNYPATRAIFERWMAWKPEESSWNAYIKFETRLKLYDNARSIFERYILVHPFVKTWLKYAKYEEKIGFIDNARMIYQRAIEFLGEDGSEEELFISFAKFEERFKEIERARVIYKYAIDHVPKSKAKDLFETFTNFEKQHGDRIGIEDVIIGKKRFQYEEEVKKNPNSYDTWFDYTKLEEASGEIPKAREVYERSIANIPPTNEKKHWKRYIYLWINYALFEELVAKDIDRTRLVYSECIKLLPHNIFSFSKIWIMFAQFEIRQLNLEKARSIFGNAIGRYPKGKILDAYITLEIELGNFERVRKLYEKYIEIMPENCTAWEKYAQLESELGETDRARAIYEIAVQQPSLDRPELIWRCYIDFEIEQKEFEMARLLYRRLLERTNHVKVWISFAQFEFNASGSNSTEKARQLYNEAHRALLDSDKEERLVLLENWKQFEEENGNQETLSQVTKKIPKRLIKKRVIQLPDGSEGGVEEYFEYIFPEEQSSLPNLSLKILERAQAWKKQKKEQEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0L0</accession>
    <name>A0A8J4Q0L0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000057">
        <property type="protein sequence ID" value="KAF2076549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0L0"/>
    <dbReference type="OrthoDB" id="18561at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035217684" evidence="1">
        <location>
            <begin position="19"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="296" mass="32885" checksum="7D0719872C7AEDD1" modified="2022-05-25" version="1">MKALNVFLGFILLQACFSFINSQIAHGPGLDLGPIRTDLQNSETQIRNLKRYDYSFCKLYIRDNTTGFEAYTTFHPRVDEFTTLKIPMSNVSIMGNENVVIWMEIPGNKSTTSFYTYNATVDVPKSCTYENGTRLTVTECTDPAVATYNVTEYVMKQGNVTKYGNIVLPPKHVHDLGKNATVNYFTVVVQLANGAITDAVWDGDRINACKDCDQCIDGQCAAKYSDLGCDIGGGCQLKIFMAWAGLDRLKKPCISINKIPSNFAKYSSTSVSKLGLGLLSDFFYRINDNNNNPNRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q159</accession>
    <name>A0A8J4Q159_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">4a-hydroxytetrahydrobiopterin dehydratase</fullName>
            <ecNumber evidence="3">4.2.1.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">4-alpha-hydroxy-tetrahydropterin dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_001797</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin = (6R)-L-erythro-6,7-dihydrobiopterin + H2O</text>
            <dbReference type="Rhea" id="RHEA:11920"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15642"/>
            <dbReference type="ChEBI" id="CHEBI:43120"/>
            <dbReference type="EC" id="4.2.1.96"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pterin-4-alpha-carbinolamine dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.96" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000045">
        <property type="protein sequence ID" value="KAF2076894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q159"/>
    <dbReference type="OrthoDB" id="277398at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008124">
        <property type="term" value="F:4-alpha-hydroxytetrahydrobiopterin dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.20">
        <property type="entry name" value="Transcriptional coactivator/pterin dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036428">
        <property type="entry name" value="PCD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001533">
        <property type="entry name" value="Pterin_deHydtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599:SF0">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01329">
        <property type="entry name" value="Pterin_4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55248">
        <property type="entry name" value="PCD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2076894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="163" mass="18793" checksum="F5D9B8238B645AA7" modified="2022-05-25" version="1">MSVCKIFSNTITKSSSSLSFFPKCLNHTTKRHYSTTNDKVPLHEKKCIPCEGDIDPLDINEKIKLLKNLDKKWTMGNDNKSIFRVWRIPFPRAIKQLQYVMQIAEEEGHHPDVSITSYWNLKIEIFTHSINDLTENDFILASKIDGLQIPDAQPPTPKKSHPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1H3</accession>
    <name>A0A8J4Q1H3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002334</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000063">
        <property type="protein sequence ID" value="KAF2076377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1H3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="472" mass="55163" checksum="0E957664B990140D" modified="2022-05-25" version="1">MATEFDELKNFISVLCTTDRTGDLLEKFNAKKSIVKYVLHQLEPTQWYACVFATMIDTDHSMNHFSTLEILYVILKHQYHQSVGKEKQKSIIVESIHILNILLGKHTTTTTEITTRDNSFYYINSIFQKLVQINNNNNNNSDYNDNEDQIVNIIIQFHSRYYRDIKSAIISIFCRQNQEQLSNNSNNSNNIFSLFQIINKILKLYVLLLSTTRSKKSKDLVIQHIMNHFSQQEFLDTVMLNNDLIQYCKNQIISPNGTLFIDNSIGGEYILLCRLYQLFLIVINNSDGSDSNSCLSIDWIEMIENIGPLFIQQDDLLIESMNLLLNYTIHSKQVMASHALFKFFLEKVCFFDHLSIIEHIHTDHQRQGQQNTCLHFLNNYLSILEQDKERFFKIYIENNGDGDGDGDVDDDDDDDEYDGSENEQEYIYIEQLLFCLKELSQEVKNSSSRWAFMREILAISCAKMEEIFFNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1M0</accession>
    <name>A0A8J4Q1M0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 9">Adenosylhomocysteinase</fullName>
            <ecNumber evidence="6 9">3.13.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_000385</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine</text>
            <dbReference type="Rhea" id="RHEA:21708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="3.13.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8 9">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="8 9">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.13.2.1" evidence="6 9"/>
    <dbReference type="EMBL" id="AJWJ01000007">
        <property type="protein sequence ID" value="KAF2078293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1M0"/>
    <dbReference type="OrthoDB" id="10007170at2759"/>
    <dbReference type="UniPathway" id="UPA00314">
        <property type="reaction ID" value="UER00076"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004013">
        <property type="term" value="F:adenosylhomocysteinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033353">
        <property type="term" value="P:S-adenosylmethionine cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00401">
        <property type="entry name" value="SAHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1480:FF:000004">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000004">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1480">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00563">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042172">
        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015878">
        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00936">
        <property type="entry name" value="ahcY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004005">
        <property type="entry name" value="PRK05476.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420:SF0">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00670">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001109">
        <property type="entry name" value="Ad_hcy_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00738">
        <property type="entry name" value="ADOHCYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00739">
        <property type="entry name" value="ADOHCYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8 9" id="KW-0520">NAD</keyword>
    <keyword evidence="3 9" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S-adenosyl-L-homocysteine hydrolase NAD binding" evidence="11">
        <location>
            <begin position="191"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="157"/>
            <end position="159"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="222"/>
            <end position="227"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="299"/>
            <end position="301"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001109-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001109-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2078293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="431" mass="47150" checksum="897EC75D8D4730A2" modified="2022-05-25" version="1">MTKLAYKVKDISLADWGRKEIEMAENEMPGLMALRKKYGPQQILKGARIAGCLHMTIQTAVLIETLTALGAQVQWSSCNIFSTQDHAAAGIAATGVPVYAWKGETEEEYNWCIEQTIVFPDGQPLNMILDDGGDLTNLVHDTYPQYLAGIKGISEETTTGVHNLYKMFNAGKLKVPAINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMIAGKVAVVAGYGDVGKGSAQSLSKMGARVLITEIDPINALQACMDGYQVVTMEEAAPQANIFVTTTGCRDIIVGKHFEVMKEDAIVCNIGHFDIEIDVAWLNANATKVNVKPQVDRYTLKSGTHIILLAEGRLVNLGCATGHPSFVMSNSFSNQTLAQIALWTESDKYPLGVHFLPKKLDEEVARLHLEQLGAKLTTLSDTQSKYLGIDVAGPYKPDHYRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3G7</accession>
    <name>A0A8J4Q3G7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001307</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000030">
        <property type="protein sequence ID" value="KAF2077379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3G7"/>
    <dbReference type="OrthoDB" id="24485at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550:SF2">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="605" mass="70208" checksum="45E8FE5D58865773" modified="2022-05-25" version="1">MCKGGACRGRLGAYDRSENKNFYTGLESFFYHEDNDFSYKKSKPIVAGVVVGVEAPSFWPVFRNISIRRRIWSLLNEPLVPYLKYYNIHSVHWLLKHGYINLFIEKVKRGDYLNDILTLSQHLSVFENINDLEFFKIFSISFKRYLLSPHMESLRAIIESKLNPTITSTTTTTTTTTTTTTTTSASTTPNNDIKEKPTNVKKRKLDQVSNTSDNKDLKSPKVETPEPEMTYDQLFEKYTTNFTFRYFKRALESKHFSIAKYVLDNHSKYEVSSFGPILPGDREIINYLCKVNNGNHMNIKIKLKNIYKLPDFEVIVKQLEEAKFIRKDDMSFMDSFETRANFDYFLANGYNHSSRIYAQIARGNPEQLTALPILLTHFKDDLFQLMNGCRESFNEHAVYRIIFNLVPQDQFVERSIINLSFEKEMKKFELEYNPTSASFENVELKDLNLAHLFSFGNLDFAKLFLDKFYTTGQLDNSHILTIIKTLVGSAHLHILEMMFKDYPTIFNFHKTGPNPSNLISVKNLALMESCLSYESFEMLDFIIDKLQICDSTSRQLAELCAGDELLLSYLAHKKRPNMKTCIVLKKDIAASERKLRVKINLLDFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q420</accession>
    <name>A0A8J4Q420_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000219</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000004">
        <property type="protein sequence ID" value="KAF2078469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q420"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2078469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="104" mass="12077" checksum="59012DD43AA671FB" modified="2022-05-25" version="1">MLVTDLFYVLQLTWRSIQNHSQLPRDCLDQAWIGNLVIDQLEYTLKASHQFLNIEFNLTVSLKIAMASLCKDFLPRAVSHLFHLVEYGVTFPIFASLVCQQRYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q432</accession>
    <name>A0A8J4Q432_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_000279</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NOB1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000005">
        <property type="protein sequence ID" value="KAF2078412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q432"/>
    <dbReference type="OrthoDB" id="446759at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031981">
        <property type="term" value="C:nuclear lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09876">
        <property type="entry name" value="PIN_Nob1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000020">
        <property type="entry name" value="20S-pre-rRNA D-site endonuclease NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.210.10">
        <property type="entry name" value="Nin one binding (NOB1), Zn-ribbon-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039907">
        <property type="entry name" value="NOB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036283">
        <property type="entry name" value="NOB1_Zf-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014881">
        <property type="entry name" value="NOB1_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033411">
        <property type="entry name" value="Ribonuclease_PIN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814:SF2">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17146">
        <property type="entry name" value="PIN_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08772">
        <property type="entry name" value="Zn_ribbon_NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144206">
        <property type="entry name" value="NOB1 zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribonuclease PIN" evidence="7">
        <location>
            <begin position="85"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Nin one binding (NOB1) Zn-ribbon-like" evidence="6">
        <location>
            <begin position="404"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="179"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="195"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="211"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="241"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="273"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="285"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2078412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="551" mass="62220" checksum="2FD5F57E33372216" modified="2022-05-25" version="1">MSFAAAILNAMEAEKRKEEQQQQQQVTEQETEEEEDLTEEQLNELNNLSINHDDDPFGQRVFTSSTGDDQKTLAITNEKITATHLVVDTNAIISGVRLETLGKELWTIPEVLEEVIDSRSKEFLDHFPFEIKTRQPSPQSVAAVTEFSRLTGDYPSLSVVDIKIIALTYTLEAEKNGIDNIKTKPAPTPVYNNNKKKEFTPKEKKVEEKPTATTTTTTTTTTTDTTTTTEASTATPTDKPQKKKRRQKKKPANKAATTTTTTTTGSVEKDTCCDEEEKDEEEENTVNNNNTNTISKKKEKKNALKVDLSKLEESEPQVDPKEVLRQLKEKQREEAEERKKNRVYQEGDEGEWITPDNLAQTVSLQRCIDETIHYDVGCITKDFSMQNVILQMNLNLLSVDGLVIKQVKQFVLKCVACLNFTLNMDKIFCEHCGGKTLYKAMTFVDKHGNHRVSQGSGKQFNLRGTIYSIPKVKGGKKHNDIILTEDQYLHRLRVTGQLYKKKASKEVNLDDLEMGFGNKTIPDDIVIGYGNRNPNIARKSIGKKNKSISIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q448</accession>
    <name>A0A8J4Q448_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_000297</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="AJWJ01000005">
        <property type="protein sequence ID" value="KAF2078430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="341578at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004694">
        <property type="term" value="F:eukaryotic translation initiation factor 2alpha kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009893">
        <property type="term" value="P:positive regulation of metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23823">
        <property type="entry name" value="RWD_GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000050">
        <property type="entry name" value="eIF-2-alpha kinase GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000012">
        <property type="entry name" value="Histidine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024435">
        <property type="entry name" value="HisRS-related_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF188">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE IFKA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12745">
        <property type="entry name" value="HGTP_anticodon2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RWD" evidence="14">
        <location>
            <begin position="59"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="394"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="870"/>
            <end position="1389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="186"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="274"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="358"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="757"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="985"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1229"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1887"/>
            <end position="1979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="194"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="757"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="790"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="816"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="993"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1021"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1084"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1125"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1239"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1269"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1887"/>
            <end position="1970"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="900"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2078430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="2096" mass="234338" checksum="161CB721210EDDA7" modified="2022-05-25" version="1">MSSTTTNSTLNNSGGSGGGGSGGSTSSSSSSASRGRLNSNSSGNILGSLDPQNDTSQQEEIECLKSIYRDGFEEFPPEGLCKRFKITLIPHPTHQYQNYNSVKLVVKYTPNYPRTLPLVELEKVKGLSDDQIDELNILLSQKMIPGEIIIFELCQAIQDFLLLYNKETVSLHEEMIQRLKERNHNNKSDFSLDDQVKSSKNDKDRDPDIYDEYYRDDIQMGGVGGGGGSDKMGMGDRYLHHHLLHHHHRDYDDDNELGNINNGAGLSMLSRGNSLGSFNQRNRSSTFSNSGGLDSGDSFQPPQSSAFSKVSSLGQRTFSFDSLNGLEYDSIFEENTSSVLSSAAATLANKSSLLGGNNAGGDGYNDDDESEHTSNSNVLNTSGGFNRQFTKIRWKQGNLLDKGSFYSCYEATNLETNKPMVVKIIDILHPNASSSGSSNSSGGGSGISSTSISGDLSMDSSTSSYNSRETLLKIYAIQKEIEQMKYLNNPHLVKYIGTLIEVNTLYIFQELVTGRTLKKILLSNRLEEALIKKYTYQMLLGLLYLHSQHIPHRDIKLQNIMVDEESNRVLLTNYGGKSMKIFDHFERHSAKNFNFWISNKTYHASKYHQRREDLFNLGIVVLEMLSGTELTKKNPIYSVLNNQLQQYLSNNNIYSIGNSNNNNNSGSNQSNNTYNNEISKLLDSTVSLSNLAKDFLLLCFTFQNSNDSIKLEAGYLLKHPFISLSNRTTTTTTTATATPASPPIPLVPNINTTTTTTATATATTTTTTTTTTNTNTNTSTIPKHITQPPINDNNIQQQILTPPQSPQMGFIRNKRPASPQTTTSVTTPQTPGSPQTPIKQQQQQQQPILPSLLPSVDMYKYLSSRYRTDFEEIEMIGKGGFGVVVKSRNKLDGRYYAIKKIKTQGYSDRDTEPLTNKLLREVTTLSRLHHQYVVRYYQAWIEQTDQSLFTDDGEIEDLSGNLETDASEDWFMQSSINSRSLLSMDESTMTGVNDSSNTNTTTSSNLDTSSMSFKAPKPTKKIMTSSSVSTSTSTSTTSKVLSTVKKKPTTTTTAGKDKKKQSADNNFFASSRDLNREFVSGSESESDSDSQSESGDNDESESGDESVAIGDSGDESRIQSEDESIYSTSKFGPTQSRVLSNKKQPAIPKKDTHTLYIQMEYCSKKTLKTLIDSVEGLNEDEVWRLFRQIVEGLNHIHSQGIIHRDLKPANIFIDTDSNVKIGDFGLATSGGPSSKSDDNNNIDNNNNNTDNNNNNNDNNNNHNRINEMDQFNDSVGNSTHHTFHEGDHSMTGGVGTPFYCCPEILQKNIKHYGSKVDMYSLGIIFFEMCHPFQTQMERSNILRDLRNELKFPPGFEALKPDQAGIIKNLLSHDPNQRYSTKQLLETGLLPSRIEDDILKEAIKTITNPTISLFTYLMDKLFEVTSDEHINNRYLYTANTTIVFLHLACREKTFQRLANIFRKHGALRIDTPTFFPKDGFLSNHNNVAQFLDEGGTVVYLPYDLTVPWARHVAIHNITQSKRYSFSKVYRRSAPGFSPKELYECDFDIVGPPKSRFVSDAEILRTVVEIMDEFSKDFGTNNYVIRINHYSILDGVLNLCGVQKKDFPQVYLAISQLHWKWNWNQVVSSLKTLGLSINCINKLSLYFKQKGELLHSVSQLETLLGNRNEAMVGIQDLKALSRNLQMINIIPKFFLDLSLIHNYSYYDGIVFQAIIERPNPDNPNLQKIEIIMAGGRYDKLIRSLHPNPAQANNIGGIGVTIASEKIVQSVLMFEQKLCKFRGIILSPNIEVLICSLGAPMLTEKLQIACQLWSMNIKSDYSQTDYTNAEEIYAHCKQNGILWVVIIKERTFQSGTVKIRHIETKSETSVGRKDLVDFILKAKKYRVMETSSSTFSTGSSNTSGSTNNSNNQSNNNQNSGGSGSGSNSNINNNSNQNNQINTNNSDRGNNINNNNNNNSFSSNSSSGNNISTSVLLNTPNPSEHEDSFNVQIFFQGNEEIKNQIKRLESSIKESVAKLFRGFVQTKASTIRVVAVDLPINLLREVSITENHDNLSKFPRALKDKLQQLKTQILKWKNIPFFVIYSYKDDKAIVYNTSCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q453</accession>
    <name>A0A8J4Q453_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000806</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000017">
        <property type="protein sequence ID" value="KAF2077844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q453"/>
    <dbReference type="OrthoDB" id="10618613at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="72"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="348" mass="39603" checksum="ADB3FC5844E668AC" modified="2022-05-25" version="1">MVDSVLTDGGVAVFGTDTADNNNNNNNNNNNTNFIYINNIYNINGGINEMLSLSPPPLLANNTSTSASLNTTATTSSISAPTTSSSSTLNSDINNSQNNMVNDDDNDSSIENDIEEEQEEPLEFIIKFRYPKSTEQKVVITRLKRSLCFNNIYPYHLRDYIPKNVFIESIRKINSKSHRPFYFIIAVHCILLLFCLGLLLVGLLPRINKVSIVIFTALIVVSFFYFIYDLLVINSRYLQNIELELDNLNTTTFQSDSIEWKLVKRSFIDFHRKKVTKLWIEINLPENGPVKSIDASCLSFSDIAISFDPNEIPIVKDHNYYYSMDSSLDSNRNSFKYNNNHSSNTIYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q5F5</accession>
    <name>A0A8J4Q5F5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA-splicing endonuclease subunit Sen15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_004385</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEN15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000154">
        <property type="protein sequence ID" value="KAF2074291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q5F5"/>
    <dbReference type="OrthoDB" id="17951at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018593">
        <property type="entry name" value="tRNA-endonuc_su_Sen15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09631">
        <property type="entry name" value="Sen15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="tRNA-intron endonuclease catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA-splicing endonuclease subunit Sen15" evidence="3">
        <location>
            <begin position="43"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2074291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="159" mass="18300" checksum="014616372C334398" modified="2022-05-25" version="1">MMIINNDIINQGLEGQSKILESIKNHFKDDCTLDQIHLCFQLIIDLMILKKWYGVQVKSGRKTNDSNEKQEHQQQQSSTPFLYLKAFETSEDEKLKKCQYVVPTKSASLWSLCTIKKYFEIFGKDSDEDGGGKELRKFTTAIQDGDGSVTYYQLSKPLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q7T3</accession>
    <name>A0A8J4Q7T3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002519</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000070">
        <property type="protein sequence ID" value="KAF2076166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q7T3"/>
    <dbReference type="OrthoDB" id="10529782at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="204" mass="23266" checksum="6C2CF3373CAC8965" modified="2022-05-25" version="1">MSKCPFHHISSATTTSTTTTTSTSITTSNQAGTTTTEADFSTLWGCQVIRDGGLNFNIKTNQFLKCTYLSKVSKEEEEEENRVSVCPFLRNESASKSSSIHCNKNTIEEEEEEDDDDDNQDNYIMDKDGCLIMPMTNIPHFLTFEEIKYILESCKIQFKDQTKKWNHLHFYKGIDINQLSQLESNTMLGDIIPTLLESESIYIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQS9</accession>
    <name>A0A8J4UQS9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_007587</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CLN5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000413">
        <property type="protein sequence ID" value="KAF2071101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQS9"/>
    <dbReference type="OrthoDB" id="18220at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007040">
        <property type="term" value="P:lysosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018939">
        <property type="entry name" value="Autophagy-rel_prot_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026138">
        <property type="entry name" value="CLN5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15380">
        <property type="entry name" value="CEROID-LIPOFUSCINOSIS, NEURONAL 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15380:SF1">
        <property type="entry name" value="CLN5-LIKE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09451">
        <property type="entry name" value="ATG27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15014">
        <property type="entry name" value="CLN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035165456" evidence="9">
        <location>
            <begin position="21"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="307"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2071101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="383" mass="43332" checksum="052FEA28D7CE32C8" modified="2022-05-25" version="1">MKIYNIIISFIILFNGICNCFEYTDTFQVYYLEAPLMYAKYGDLLSHINAFHSGVGFRSIYNASEQFTVDFTAQPSIISSLFPASIVNESLRTEINWDSYATVSIQTLINDTYWSSQQHIMNCTGEQLAQYLCWSPTFNNTKYNLFDVRDAESNQVYLPSRICNDFAWSSFDALFNLGATVVSNLKPSRDYITLYVTQEPVKIEYEDEATWNEITKFYIDLEKLKEMNESMTSLVKTMDHLFNGTFFLYVDDSYYNLSLAVFKTPISFTYTPTPLPSGTPDKSSVGYNSSCYSPLNPNKPSSISGGWIFIIILSCGVSTYLILGVIVNKFLLKQSGLDLIPNRSFWSGFGVNISAGFYFIKSKITGSSSSQYSYISSSSSDNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UR68</accession>
    <name>A0A8J4UR68_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_006982</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000348">
        <property type="protein sequence ID" value="KAF2071701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UR68"/>
    <dbReference type="OrthoDB" id="21498at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043226">
        <property type="term" value="C:organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000072">
        <property type="entry name" value="Unconventional Myosin IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="306"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="56"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="144"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="189"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="229"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2071701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="364" mass="39954" checksum="6D2D30F34B3363D1" modified="2022-05-25" version="1">MTNKLVGNYLDVRNKKYNEEDDDDWDTDADFVNESNIDNSKQTTKPSANVRASIVGRDLLEKQAPIDKVPTSPISKKPAFQQTTYTPPGVVINKTAAKPAAPAPTPAASKPSSVGSVASKFNQPAAPAPAPVKSTPVVSKFAQPAATPAPAPVKSTPVVSKFAQPAAAPAPAPVKSTPVVSKFAQPAATPAPAPAPVKKPPPPPKRVPEPEPEPVYEPEPEPVYEETYDQQQYDQSGYDQQQYDDQQQYDQSGYDQSGYDQQQYDDQQQYDQSGYDQQQYDDQQQYDQSGYDQSGYDQSGYEEQQTQYTQAEAIYDYVAQQEGDLTFYTGDIIYVYDTSDESGWWQGEVNGAQGTFPSTFVKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4USA7</accession>
    <name>A0A8J4USA7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RhoGAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_005309</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000207">
        <property type="protein sequence ID" value="KAF2073396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4USA7"/>
    <dbReference type="OrthoDB" id="437889at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45876">
        <property type="entry name" value="FI04035P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45876:SF8">
        <property type="entry name" value="FI04035P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-BAR" evidence="5">
        <location>
            <begin position="62"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="4">
        <location>
            <begin position="493"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="390"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="455"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="697"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="785"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="397"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="698"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="724"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="746"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="791"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="806"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="823"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="848"/>
            <end position="861"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2073396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="877" mass="98931" checksum="D35181B37DEC3F8B" modified="2022-05-25" version="1">MINSNDSTSTAGGNSNNNTAPTLDALKEVQLLSPLRSGQQDINCDNINLIAPTDLTSTASILSFSNDLWDGYDILCKRTELGIEQCKDLLEFFKRRATIEDKYSKSVTEYFSRFKLKDENDSFQKGIQLITKVCEAESNVHKTFSSNLLNNLCHPFSALVKEMEQRRKKLIQEGQKVRNDLKDSIELLKKHHLRYEKQCKEMESVKVELINMIEKEPDANSKVQALERKVAKCESNAIIAEEEYKSQIKDTNEFISTSYLIRMTDNLNEFEQFESMRLQFIKSNIKNFIGLKGEVEGETISNTSIDVIDPELDIQSFIKNHLSNRKVIPPFQFEPYVEGKIISLNSSNNNVFNNSGFVHSNNSSSFNSTSTAGSTKDTLFNGSSNNSFLKQDSFKENSNNGGSFNSNINNNSNTSVSPPQTSTKSIKENIFGFFNKATNNLKSSSASIINSTFNDNNSNSNSNNNQSSTNNNNSNNNSLNKSLNNICLSVFGVELEEIMDHQKKTFPNLEIPWILIEFVKTLSKLNAFKTNGIFTISPSHQMVQQEKQRINEGGSLDSITDVHLLATLLKHWLRDLPNPLISYAIYDEIIESPEKAWSILETGIPLLHRKVLIYILEFLVTFIQPEFTYISKIDMHELATLIAPVLIRPSESKDPEKALVNSHKEIQLLESLLGDCNEKKKSNNQFLKKRQTVALSLETDDNSDDEYDENDKYDQDSLSSDNNNNDDDDYNDSVDDDDDSDDDNGNHNNKNNSNYNQSKSTNRDKIIQNYKQRNQHLVSKQSKFANHSDNDNSSSSGSSSNDNTPHHQYQFQITPNNFTPILSPNDFDPSSPASSSQIPPRSSSTLSFGNSDTFSVTQSDSDPVLVECEHISDGFDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4USW5</accession>
    <name>A0A8J4USW5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tafazzin family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_004615</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1'-[1,2-diacyl-sn-glycero-3-phospho],3'-[1-acyl-sn-glycero-3-phospho]-glycerol + a 1,2-diacyl-sn-glycero-3-phosphocholine = a cardiolipin + a 1-acyl-sn-glycero-3-phosphocholine</text>
            <dbReference type="Rhea" id="RHEA:33731"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="ChEBI" id="CHEBI:62237"/>
            <dbReference type="ChEBI" id="CHEBI:64743"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:33732"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:33733"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Mitochondrion outer membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
            <orientation evidence="10">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the taffazin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000166">
        <property type="protein sequence ID" value="KAF2074086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4USW5"/>
    <dbReference type="OrthoDB" id="193467at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047184">
        <property type="term" value="F:1-acylglycerophosphocholine O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035965">
        <property type="term" value="P:cardiolipin acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007007">
        <property type="term" value="P:inner mitochondrial membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07989">
        <property type="entry name" value="LPLAT_AGPAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000872">
        <property type="entry name" value="Tafazzin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12497:SF0">
        <property type="entry name" value="TAFAZZIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12497">
        <property type="entry name" value="TAZ PROTEIN TAFAZZIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00979">
        <property type="entry name" value="TAFAZZIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="13">
        <location>
            <begin position="69"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2074086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="312" mass="35640" checksum="B376CDA96335351D" modified="2022-05-25" version="1">MNTNSNSNNNERLTRPIYDVPEPKIVSRVLMASVGLLCKFWLGFNTVTVHNGDLLTQQVEKTHKERNPLITVCNHISNLDDPICWGILPVNLICNASSARWTLGASNILFSNQLRSKFFELGKCIKIVRGDGIYQEGMTEVIDRLTEGQWLNIFPEGRVNQSGSLLYFKWGVGRMVAECFRKTGITPTVLPIYHNGLQHSMPLGQKAIPRPFQKLDIFVGETIDCEPTIKQFLFDNNLDNLDCLQGNEAKQKELYKNITTHIQDRFQSLESNSKNKLTLRERLEQHQHKIAGKNESPTFTSLLNQDDWRLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVF2</accession>
    <name>A0A8J4UVF2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DDT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_010078</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the importin alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000914">
        <property type="protein sequence ID" value="KAF2068595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVF2"/>
    <dbReference type="OrthoDB" id="21522at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018501">
        <property type="entry name" value="DDT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50827">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DDT" evidence="6">
        <location>
            <begin position="306"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2068595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="430" mass="48968" checksum="34C838674D0EBBF9" modified="2022-05-25" version="1" fragment="single">AWCITNISSGGHDETSTVVACTNVLVNYLSSNNKTLQDQCAWALGNIALDCKEYRDIIISQGVIRPLLKMLSTSTVNNILTTVCFTLSSLFSYPVPNTVKKFPINEKDIFQFMFDINDHCKRLKESINSNDNNSNISSISQHSIVLDHILWTFYDILLYIPESILVSLKDNQLFITLTLLVCDLSDIINSNNSNIELNFTLPLIRIFGLIVQQQDILLHIVKELFNSNQCLAFIKSIFSLLNTSNSSNNSNTIGSTSIIKESFYFLSNLTCVLGRDQFRFVDKLLNEPVILILDYLISNQSFQNDLFNVFDLFTLYEFLILFSNSLAFSTLSTISTTTSTNYSKESVDIMALLYLDLLKAQYQDKDTDTDIDFDQDIDLQNLCLNFLSFYSNMFGIKNNDSVYQKCKEIKQMLSENKNTQLITFLEKVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UW41</accession>
    <name>A0A8J4UW41_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">H/ACA ribonucleoprotein complex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleolar protein family A member 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001546</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Common component of the spliceosome and rRNA processing machinery.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Required for ribosome biogenesis. Part of a complex which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ('psi') residues may serve to stabilize the conformation of rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000037">
        <property type="protein sequence ID" value="KAF2077161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UW41"/>
    <dbReference type="OrthoDB" id="5364946at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002415">
        <property type="entry name" value="H/ACA_rnp_Nhp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00883">
        <property type="entry name" value="NUCLEARHMG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="2">
        <location>
            <begin position="32"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="142" mass="15711" checksum="FF72456FD0507C5C" modified="2022-05-25" version="1">MSSDSESESTQKKLYLSPIAKPLAEPKLTLKLLKLVKKASKDKKVSRGVKEVVKHIKKTKDSKNKNRICIIAGDVTPIDVISHIPVMLEESGIKYIYVPSKESLGTASSTKRPTSIALVDLDSESEHKKLLKECLEKVPSEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWH9</accession>
    <name>A0A8J4UWH9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_009097</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the trehalose phosphatase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000636">
        <property type="protein sequence ID" value="KAF2069583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWH9"/>
    <dbReference type="OrthoDB" id="755951at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01627">
        <property type="entry name" value="HAD_TPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000010">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000052">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1020">
        <property type="entry name" value="Trehalose-6-phosphate phosphatase related protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011071">
        <property type="entry name" value="PRK14501.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00685">
        <property type="entry name" value="T6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF16">
        <property type="entry name" value="ALPHA,ALPHA-TREHALOSE-PHOSPHATE SYNTHASE [UDP-FORMING] B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2069583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="793" mass="90880" checksum="996998BBCA35B3FB" modified="2022-05-25" version="1">MTIQRSTSLNNIINEKLGRIIVASNTLPMSVKKVDESSCNSSLSNTPLSSSIDTGFTSNGISTPEQKSPIQIKISDEPFPVESALSSLLHKKEISDYLWVGWPHCDVENEEEKKLIEKAIKEHSSHLEPVFLDEKLVDNYYKGYCKNSLWSLLHYQLNFVKLDVEWWQAYIQVNQMFADRISKIYTPGDIIWIHDYHLMILPEMLRKVLPVDAPIGFFFHAPFPSYELFRILPNRIEMLKGILGSNLIGFQSFEYLRHFRSSCARLLDLEVHPKGLELVQGRRTHFTKLQVYPIGVDYSDFIVNLNQPIVTSRIKKLKEIFKGKRVIVARDRLDQIEGVPRKLEVIEHLFETHPEYLGKLVFIQIYEPTVEEEDETDEQKLLHKTVNEMVGRINGKYGKLNFNPIEYINRKVSYDELSALYKLADIALITPIRDGMNLTSHEYVVCQKDSYGVLILSEFTGAARCLGGAIIVNPFSKKEIQGAIIEALNMKIEDRKLKHQINYNYVLANTSGFWGKRFLCDLHETIQQDCNNNKKVPCADTEKIRENFKLSTKRLLLLDYDGTLTPLVKHPHLALPSKQLIDGLTALSKDPRNEVYVISGRDRPSLEKWLGSIPIGLSCEHGVFIRHPNDQKWYETPKSEIAWKDTVMNIMQDFEDRTPGSFVEIKSINLTWHYRNADPDFGQFQARELIAQLHSVANKYPLDILSGKKAIEVKPVGINKGEIVKILLARCDPEFVLCIGDDKTDEDMFKVLQDKADAFSIRVTPDNKEPTKAKSTLEETKDVVNLLTQLSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UX24</accession>
    <name>A0A8J4UX24_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MJ1316 RNA cyclic group end recognition domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008722</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000566">
        <property type="protein sequence ID" value="KAF2069958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UX24"/>
    <dbReference type="OrthoDB" id="19211at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053019">
        <property type="entry name" value="GATA_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040459">
        <property type="entry name" value="MJ1316"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23353:SF32">
        <property type="entry name" value="AAC-RICH MRNA CLONE AAC4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23353">
        <property type="entry name" value="RAB-GAP/TBC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04457">
        <property type="entry name" value="MJ1316"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MJ1316 RNA cyclic group end recognition" evidence="2">
        <location>
            <begin position="147"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="229"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="268" mass="30797" checksum="E0E484A2DDC55A66" modified="2022-05-25" version="1">MATNQQEQQQQQQQQYNQDYDYSSSEEEYDDEIMDLEEQLQGGGRYRSGSKSSTTSSATSVSSSKKQSTATRKNSRSTPVVNNNKPSSPTKDLPDSTATASDEETDLYGRKQQQLKEEKESNHLSASEVKEILDKEIECTKKKRLPSSEEVYNRIKWEAPAYGVDVDTFIIGYIDRFVDLMKVPFSAFEIGVIPLHRVMKIYYQDKVIWDRKERLFEFPSFGNDQDDSNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNDIKIDNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXD8</accession>
    <name>A0A8J4UXD8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000504">
        <property type="protein sequence ID" value="KAF2070348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXD8"/>
    <dbReference type="OrthoDB" id="417426at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF180">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="499" mass="57202" checksum="CF49E69C2C329F03" modified="2022-05-25" version="1">MSSEIINPSNRPKSSVSSFFILFNNQFIKWKIFSFLSHDLDVTIRADYAKNNELFSNFSNIKLRLVKGHSVRSLIMDNNSHLNIRSLDIVIENEATPFKYLPKSVETLCLVFQCEPFPLNKGFLGNSISTLSLSGRYDEVCLNLPPSIRKLMLNGRVALPPSLPKYLQHLELDGGFSEKITKEYQFPQTLEYIKFGSNYSHSIYHCTLSHIKTVVFVRKYINLALPTGEMTYFCPISYLPPPVSDLVCLKYDDRDTIVPLTHLPTPLPQNLRYLDLGDVFNDTLDDLHLPDNLTTLKLGNEFNQCINHLPAQLRHLTFGQCFNSTINQPLPRQLEKLEFGVDFDRPITSVLPDTLLELKFGRRFNQKLEIGDLPSQLQVLILDDKYCWPIEPRVLPHSLKRLKLGSKISEPFEFNVIPHGVISLSFGGIEGFYLLPGVLPCSLRYLQMDIGAIQTKTICLPNGIKKLSYQFPQGNLRFYAKDDRRLLKNRKDFNFFSSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXI8</accession>
    <name>A0A8J4UXI8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Band 7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_008133</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000477">
        <property type="protein sequence ID" value="KAF2070543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXI8"/>
    <dbReference type="OrthoDB" id="434619at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08829">
        <property type="entry name" value="SPFH_paraslipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000004">
        <property type="entry name" value="Putative membrane protease family, stomatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050710">
        <property type="entry name" value="Band7/mec-2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43327:SF18">
        <property type="entry name" value="BAND 7 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43327">
        <property type="entry name" value="STOMATIN-LIKE PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Band 7" evidence="4">
        <location>
            <begin position="53"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2070543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="360" mass="40182" checksum="C99E12DA9F4D4A5C" modified="2022-05-25" version="1">MPYVIDGSSSNEDSGSPNNSSNEPLEENHVALAIALYIIVFTFLILALIINKKVVKIVSRTQVMIIERFGKYHTTLNPGLHFLLPFIDSPKMIHWRHLDVPTGSQKPQVVLIDTDRIDMREHVITFGRQHVITKDTVQINIDALMYIQIADPKAAVYRVQNLPDSVELLAQTTLRNIIATLTLDDTFSSREFINAQLKEKTIKDAERWGVIITRVEVMSIRPPNDIKEAMEMQLTKEREKRSHILNAEGEKEGLIIKSKGMAAKIVLTSEAEKTVATQNAKGIAESKRLSAGADVEVIRLIRKAINNPDVSTTGYLVTSQYLDRLSSLPSKDTNLILVPESSLNFASNISTSIGSDKKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXK5</accession>
    <name>A0A8J4UXK5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">6-phosphofructo-2-kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_008079</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000469">
        <property type="protein sequence ID" value="KAF2070603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXK5"/>
    <dbReference type="OrthoDB" id="267323at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003873">
        <property type="term" value="F:6-phosphofructo-2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006003">
        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000006">
        <property type="entry name" value="6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000644">
        <property type="entry name" value="GpmB, Fructose-2,6-bisphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003094">
        <property type="entry name" value="6Pfruct_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013079">
        <property type="entry name" value="6Phosfructo_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606:SF44">
        <property type="entry name" value="6-PHOSPHOFRUCTO 2-KINASE_FRUCTOSE 2,6-BISPHOSPHATASE LONG FORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01591">
        <property type="entry name" value="6PF2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000709">
        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00991">
        <property type="entry name" value="6PFRUCTKNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="5">
        <location>
            <begin position="125"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="343"/>
            <end position="350"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="401"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2070603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="534" mass="61873" checksum="EB9D541E023437BD" modified="2022-05-25" version="1">MTTISITPNTSPKQQEQQQQQEQQTSNTTPTKRNSIVEKEIFFRVKDRVKKAKFKGTCIEDLKQLFLLKFPEFPKDVEHPFYIVEKGTGKEALICNIEDIQEYQEILVRHEYEQDTRQKSQGYTGLDKEKIVLVMVGLPARGKTYVARKICRMLNWMLVPAKVFNVGEYRRLRIGAGQPHDFFDPYNPEGIKARLHMAVAALDDMISWLHNGGRVGIYDATNSTNERRQLVEKRCQREKMNVIFVESICNDLDVVEANIKETKLLSPDYQGVDSNKAVQDFRERIAHYMKVYETVEGETTRYIKLYDVGKKIEVNNITGYIPSRVVFFLMNLHIHPRPIWLTRHGESEFNAQERIGGDSDLTERGDTYAHQLAVWISEWASKVRADGYDGEVVVWTSSLKRAIRTAQYISQPKVVMRALDEIDAGICDGMTYQEIQEKMPDERAARESDKLQYRYPRGESYEDVIQRLEPLLLELERTKLPVLIVSHQATLRCLYSYLHGKVKEECPFLNIPLHTLIQLTPKAYGCEETTFKLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXR3</accession>
    <name>A0A8J4UXR3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Alpha-type protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_007916</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000448">
        <property type="protein sequence ID" value="KAF2070768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXR3"/>
    <dbReference type="OrthoDB" id="301415at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005826">
        <property type="term" value="C:actomyosin contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016905">
        <property type="term" value="F:myosin heavy chain kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031037">
        <property type="term" value="P:myosin II filament disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903013">
        <property type="term" value="P:response to differentiation-inducing factor 1"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16968">
        <property type="entry name" value="Alpha_kinase_MHCK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.200.10:FF:000002">
        <property type="entry name" value="Eukaryotic elongation factor 2 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.200.10">
        <property type="entry name" value="MHCK/EF2 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004166">
        <property type="entry name" value="a-kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051852">
        <property type="entry name" value="Alpha-type_PK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45992">
        <property type="entry name" value="EUKARYOTIC ELONGATION FACTOR 2 KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45992:SF1">
        <property type="entry name" value="MYOSIN HEAVY CHAIN KINASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02816">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00811">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51158">
        <property type="entry name" value="ALPHA_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Alpha-type protein kinase" evidence="11">
        <location>
            <begin position="43"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="480"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="507"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="563"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="588"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="628"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="315"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="407"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="316"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="347"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="426"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2070768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="739" mass="81686" checksum="7D6EE41FCFC818E2" modified="2022-05-25" version="1">MEEKKPQNSAAASALALLNQKFPVADVIAGTKCERAIKWELSIGDDLTPKWTHSIVCVSIEKTPFAKGSCRTAHKLKDWSNPDKPLVGKFSTNKKTTRDSYFTDVLMQTFCSKWAEKFNEAKPPKPITFLPSYVYELIDHPPPYPVCGGEPFIEGDYKKHNNNSGYVSSDARNTPQAFSHFSYELSNNELLIVDIQGVNDFYTDPQIHTKNGEGFGEGNLGETGFHKFVQTHKCNPVCDFLKLKQMNNQTKKTLLRGTLPVVQLMEFHDAIGLNGGGPSKGYDMNYFRNGGGAQQPISLDDEEKMLQEQLDKIRLQQQQKASKPAPQMVKQPSNSNLKKTAPVSGVSPVAAKPTPKSPTAPLSPNSAVSTSSHTPSQQPSAAVPVKETPAVAQPVAAAAPVTTPVSIQVRTSPPPQQPINVQSQPTPQPTPQPPAQQPPAQQAPIQEVAPDRSAFEKWDLKNIRNSDTIRGLQSECITGDDNNIYSGSNDGQIGIWDVKESKHISNIKAHGKSVRAIIKDTTEGRLLTAGADSYVKLWDLATQTCIKEIKEANEVNTIFLQDNLLYTGCNDKTVKVWDMRNFECVKTLSGHTRAIKSVCAMGNLLFSGSNDQQIYVWNLSTASILTNFQGHEGWVKTLYAHNNYLYSGSHDETIKVWDLKTTRCVNTIKCKDRVESLHVTNQGIFAGSGDYLQVFDTTSYENLASVNTRSSILCLWRRQNQLFTGSLASNLKVWSWDVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYI4</accession>
    <name>A0A8J4UYI4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Aquaporin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_006830</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000332">
        <property type="protein sequence ID" value="KAF2071863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYI4"/>
    <dbReference type="OrthoDB" id="3222at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019755">
        <property type="term" value="P:one-carbon compound transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000069">
        <property type="entry name" value="Aquaporin-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45665">
        <property type="entry name" value="AQUAPORIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45665:SF9">
        <property type="entry name" value="AQUAPORIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2071863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="289" mass="31023" checksum="23434BCA44EE0519" modified="2022-05-25" version="1">MAEDDYNELDESTINMEKEQEEEKHMDPLEEQTKKRWVLIRAVLGELLCTFLFIYVVCSTSANFVRMKTVANPVVSAISTGFAAVALIYSFADVSGAHFNPAVTFATCVTRKTSITKGLMYVGAQLVGSVLATLILLATFPGDAYEGGSVAYAVAVAPAEDANIGNAFLTELILTFILVYVIFAVAFDTVDDNVKTVVRGGKKSGGNNLTIYTTSGATKAGFAPIAIGFTLGFLCFLGGSVSGGAFNPARVFGPALVGNKWTAHWMYWIADFLGAGMAGYTQRFFQKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZW5</accession>
    <name>A0A8J4UZW5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rap-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003787</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000123">
        <property type="protein sequence ID" value="KAF2074910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="19311at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11210:FF:000001">
        <property type="entry name" value="Ral GTPase-activating protein subunit alpha-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11210">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035974">
        <property type="entry name" value="Rap/Ran-GAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000331">
        <property type="entry name" value="Rap/Ran_GAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027107">
        <property type="entry name" value="Tuberin/Ral-act_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10063:SF11">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN CG5521-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10063">
        <property type="entry name" value="TUBERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02145">
        <property type="entry name" value="Rap_GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111347">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50085">
        <property type="entry name" value="RAPGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rap-GAP" evidence="3">
        <location>
            <begin position="1375"/>
            <end position="1585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="587"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1115"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1613"/>
            <end position="1637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1118"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1619"/>
            <end position="1637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2074910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1637" mass="185247" checksum="20056704D14FF255" modified="2022-05-25" version="1">MNSNGVNTSGGVTTTSSTNTTSPSLVSQVGPNIPISPNTALNNNVTPTSEKTKEKEKAKKKLAIFLDINLDSFKRFKGLSSFIANSDENEIDKVFKENSYQVYQTMLSTFSAYEIGTYKGKQHYAEKEVLKLMELLKRILIHLEGFVRKGWQVKSIINVLEKMLGIENIQSLRLSGFSVLMVFLEVMEKPDKYKLELFSSCIDFTPYIADYISKINLAKKVFASQELKRFIVIQSTEPPTKEDSTTLFEQIFVHMDKRLPNNFHFWFELLKVHYLSILYPNICKQNGILPPNDITGFVSHCPHELQVVIINYLSKWLTSSGPSNSSAIKDYFLYNIIPTNTAGSIPHSPSLQSVVDSAATTASSAQQQQQTQRISPSGSFEQFTNTPGRGNISILLEIFKQSCRLPLKYYEVIKKSISTFKHLFLENLNELDLGEDLNTYQTFIFNEMLHVFETESATYEKEREIIGTYIIDVYKYMIENYQSFKPNLKEIALNSMLQGTVALLRKSNPNKTVSLTLEQNIVSTTLYGWIKSKETNGKMWDSFHSSFEEIFYRPEVIKQIRSKLMQVTLVLKDLVYPLSQRKIQKKAKDIRNTSKGGEPKSLDHQDVPAEIPADPAIATAIKWDVESCNWIWHCILDLFKNLHRIKETNIHELATHILVDIVDLFIRTEVEVDFADSLDETRPAHLSLINIFGPRLFDTCLLDSKFIKSKVLALGCLCRLVCRHHPQYPISILSHFFGVINNAMISPPVNGVDLSWSIILNSSNIFNLSIPGANILIPTFLYKIKCILTLKNGELNPPSEVRKKCIIIVCSLIFYPNQFPNMDIYNQREVKGKLLGNDLTMAEMKKDIMEILHFTLKSDKQLENKLICTWGLSVFLMEEFNCNYNQDLINDIIDAITSNTLNIESSVARAALDSLSSIGLIFNKLNKPTINRILVSLCTSISKTLPEIFEGTSAVTEQTIAGHFHCLLDWISMDNSIFDDSIYSEFRTQMFYCIEEGLGLKGDYWSTTGVPVKQESVVTSTDSKDQLKSTTKNLTIKKNLYHKIKGSSSSSSNSTPAEEEPQDTTTKFLVIHDACETLLSHCFNFAHNFPSKEGAEFISSFIDEEDDQLFNEIEEGVNNSGSSNNNSNPNSNSIITNNQTHIHTLPDGKTLSSLPLFCVLNDNALISVVEIPKPNGTGTLARLFIRDATGKYVWNFDCDYDLEKQLNSNLQMYISNINDIIKDSNSSTIIFDQNQNAFKYINNVSQSPTIKPIEPDEKLNNLLLSLSKEHPECLPTSGVFLNNPLINLKSSLINEFTKNQGEINSFIERENTFNIDKYSLPPSGNSWSLNLPENFKPVVSQSSRLLMSYFGFLDFNISSNTLKQLESTNKLSRALTQLDITPGREILKIGVIYTAEGQDDQKDILRNDKGSDLYREFVDGLGWPVDLTTHQGYLGGLDRKKSTGITAPYYATPSIETIFHDITLMPTNPIDSQQIHKKRHVGNDIVNIVWSEHIRDYSPTTITSQFNDALVIVYPLSNGLFRIQIYRKESKVPMFGPLIHGMAVNKQLLSLLVRQTAINAYRYVRYNTPNYSKPFVLRKLRIKEIVDRYSSERNYVDYVHSIVSGSSNFQQAPPVTSLNTSNDNSAQTTTTTNTSTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0K0</accession>
    <name>A0A8J4V0K0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002893</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000085">
        <property type="protein sequence ID" value="KAF2075810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0K0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="580" mass="65313" checksum="8AF5EA22F524FD89" modified="2022-05-25" version="1">MIGFLLRDILFFQIWRDKYLYKTIISFNDYDLISNAFEKRHAEQRVDRYKEFNSAKIRFKLNQEGDENVKLDDIKIKNLVIEGDNDRYLKVDGKMVPESVRFLSILPFQDCSIEIKSLDTLVFVTHLTFGYFFNGVVSKSMLPPLLTHLSFGASFNQYIGEDILPKSLTHLSFGSAFDRFISPNTLPPHLRYLSFGSKFKQSLAHVPQSLTHLVVGLSFVWEKGFEFPQSLLYIEFGKGLNMEGSLSGFKLPPKLVSLSIGPWMNTSIPMGFIPSSLRFLKVGACATRFDLEKDSLPASLTSIEFAKDFKLHIDQDIIPPSVTNVKWGLKYSQPLTVGMFPLSLIKLSFGYDNVSLPIPKGFIPDSVTSLELHLNSTNQQHLGEIIPSGVTYLSLSTTYPIPVGTIPNSTKYLVLDNNHPLVPGLIPDSVTHLTLGPSYNQPIQANSLPSSLKSLSFGLFFNQELTNLPSSVVSLSFGESFNRSIDTLSTLTPNLKSLKMGLKFNQPINSLSPKLKQLRLNKTSFNSLISSFPSSLEHLSLPPLYSLNLPPYPSLTTLEYREYRMKNHPYPSTIERLKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0P7</accession>
    <name>A0A8J4V0P7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Gelsolin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_009170</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the villin/gelsolin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000648">
        <property type="protein sequence ID" value="KAF2069512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0P7"/>
    <dbReference type="OrthoDB" id="6375767at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051693">
        <property type="term" value="P:actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008154">
        <property type="term" value="P:actin polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11290">
        <property type="entry name" value="gelsolin_S1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11289">
        <property type="entry name" value="gelsolin_S2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11292">
        <property type="entry name" value="gelsolin_S3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000043">
        <property type="entry name" value="macrophage-capping protein-like isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007122">
        <property type="entry name" value="Villin/Gelsolin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977:SF130">
        <property type="entry name" value="SEVERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977">
        <property type="entry name" value="VILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00597">
        <property type="entry name" value="GELSOLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00262">
        <property type="entry name" value="GEL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0117">Actin capping</keyword>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Gelsolin-like" evidence="5">
        <location>
            <begin position="53"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="5">
        <location>
            <begin position="173"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="5">
        <location>
            <begin position="289"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2069512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="365" mass="40576" checksum="D7DC388BA96AB086" modified="2022-05-25" version="1">MIKTKKLDIGDSNISRMGSELDLKCRVDKAQSESQWRNAGKQGGLFIWRIENFQVAPIPNDQHGKFYEGDSYIVLHSIQQGASFKHAIHFWLGEFTTMDEAGTAAYKTVELDDYLGGGPVQYREIQGSESKAFLSLFPNNTIFILKGGIDSGFQKVKTEEYKSRLFHIYGDKHVRVKEVDLSAKSLNSGDVFILDTGKVIYQFNGLKSSGQERTKGATLCRAMDEERKGKATTVEVYTEGDADIPDAFWNALGGKSAIAPPIAQAASVKFDKVLFRLSDATGKMVFSQVAKGVISKKLLDTKDVFILDVGHQVYVWIGSQTSAEEKKQAFAYATDYLAPLKRPETSVIKILETGDTEEFKVQFDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1I7</accession>
    <name>A0A8J4V1I7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010501</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001155">
        <property type="protein sequence ID" value="KAF2068174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1I7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="193" mass="22678" checksum="D84E925F4584C684" modified="2022-05-25" version="1" fragment="single">FGNHIIASQANGTLTVNFYEIYDYVGTLLTMDIDLYNCLKKHSKNPYPFPLKTKLEDLLKRSSLTHAKLFLQAIKTHEPLAYQDIKDTIKDIVMEIRARNYPIKTLLFMFQEFQGLFPESILEEMVSAALSDKQFMLVDILLHKYKIRLIRPTSKEHEQQLYLKSYNKLMSSPEDIRETYLKRVPLSYIHTNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1P8</accession>
    <name>A0A8J4V1P8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_008012</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000461">
        <property type="protein sequence ID" value="KAF2070667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="26095at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1306"/>
            <end position="1329"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="4">
        <location>
            <begin position="419"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="3">
        <location>
            <begin position="796"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="2">
        <location>
            <begin position="1078"/>
            <end position="1279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2070667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1345" mass="148187" checksum="98EF6580A83C28BD" modified="2022-05-25" version="1">MMHNVADYDYNRFNDQLSLFYVFGYPSQRYYRGEVGDVGTCNYAFNVVMLNSYVISPQVSCVVNNIASASCVATPFATNSTYKATTDTFYVLTVSLNTQNTPIELDIKMNYQDSTFSNSVMVDYISLEKVKVACYDQPTTSFTGEIFETQLIQSGVGVIDNLNLKGYIKLPSSFTNVATYFPDAISPIDTTSFEHVYNNVYQVGMKLAISDPNILSELQFILGSSNSLFKFKNPWDGPVNQIIQYNRSKYDLSNGEIINEFDILFENGYVGPISHPITVLGGVANKAFSGFVGGTPPLSTYNGTNKVLDYFSITPSKTFTHNLLFTVQKYNGPVDPSQILHCTSPFELANTSFTFAFGLYNAPTEYTYPFGLVRVGQISTFSISSFFTPLGAPGRSITYIVNDNVVLTTISNSIGGYEVPPTLASMSVALLEEGYSSIKIKCTDSVSGVLYISLNMAGSEDIITLDSTHRIVGDPKNGVYEKLVKGHYLNAPYTVSIFDFANNALTVGRERLFYYYGVSTPSNTDIKLLEFINLLSVSNNDINVTNKGSSCRVNILADSSLPYQATNSFMLVLELNPLNPPVKAHSIFNSTTNMYQIDFTIPAGLMTGTIAYTLVYNSYLAKASHSMVNAKFGANSQIRVTSTPTFDHLFPVVEGVSGEQDMSLYPGKISLKWSISFSDTANFKSGSVTFASVYNKQGFTLPISANNPTFTVEFQLEIELNNCKEYDPTEFYYIQSIETEDVLGNKGKTVRWTNKTIHPFYKFDAVTDDYIYVKCLTTNDISLPQVNFVKSQFPVLDTTSSSSRNVEIDFNVFDDTSPISKTHLPVCYFLSLPSTFYSITAKIKEVVDTKNITWTCLGSLPYGFGSGYLIPVSIYGIYDTALNYIGYSAYDLATEEHLSYIDARVATAYEPFIQSTSTFYSTGGTLIIYGNSLGPQYRLVYLEQSTLSFICTSTTSTYIKCEGLPSTTVSFHISLNGESAVSNIFIVNPVLVAPDINTPSPTPTATSTPSCSSDCGEPLNYGKCINGACVCIPPHNGFDCKANIDNSTVIKPNPTQPTVNITIPGTSSGQTPQFTSFVSVVALHELDSAGTLLNNYQFNSDKWILVNEGSSSNDQVTTVQYKYVIDNSLNTTIVSTVQVFAKVTNITFGNQQLYMNPSTIKFTFNITSYPFTKSTNALQVIMTAALESSKKVACSYKEFIDDQSNSQYLRIQIEDRSLFGRFIKFGMVDGREQIITNTVLDSSYGGKDLSTSTSDQSYIGLNVPYYTKYALLDPDFSVLVETRSARDQANSVCTSESSKKLTNAQLAGIIVGGVVFVFIVCAIAIYLYTRKSTSPIAMKLRRIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1Z6</accession>
    <name>A0A8J4V1Z6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_000962</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000021">
        <property type="protein sequence ID" value="KAF2077715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1Z6"/>
    <dbReference type="OrthoDB" id="16045at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF194">
        <property type="entry name" value="INVERSIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="196"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="229"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="365"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="399"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="477"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="512"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="542"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="577"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="618"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="651"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="718"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="823"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="856"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="889"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="922"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="956"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="53"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="288"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="782"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="294"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="788"/>
            <end position="800"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2077715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1019" mass="110575" checksum="5C095BEC8CAB1D86" modified="2022-05-25" version="1">MDDHYSVNTASSGSCSEYLQESPSTFSEHLNYGSPIDIHSSLLNHHHHIGSPTESNFKMTSDDSCGSSNSNTKQTNKEIEKDKDTLTITTSVAVSPQSPSITSAIATTTNTSNSNGNTLKNNNSYSSFLESIKNDDKEFILEFCKGNKNINKLEFRDTDGRSPVHYCVEYDSFSCLETMLSIVTPLNEIVNLKDKDDWTPLHYAALFDRIECALHLIQNGANINCLTNESWTPLHVSAWRGHSDFVTMLIEKSARLSLKTKDGLTSHQLALNSSHFSIANQISEALLTKDDGGNNSNSSSSSSNNSNNTNVNSNSNSNNNNNNNNIIEETNSNEEILSLFKRASTDLDKLQLEQGVNYNNIKDSSGITPLHLCALNGLIDTIGLLVEKYNMNVNIQDNSGKTPLHYASYAGRVESMRKLISFGANVNICLLDLSPNILLQQQQQHQQQGSGEKSAGVLFVNNNNNSNNVNLKLKKEYGITPLHEAAYSGELAAVCLLIDHNANMNAKSFYGTALHYAASVGSNECIKYLLQHGADSRLRNEQGMTALHVAAFHGYPEALNTLILSNGGAEVNAKCKDGSTPLLKATMGASGSESKENSLSCISLLLDKGADANIPNDQGETPLHVSCYYGLTDIAQALIGRGSNLEAKDEWAETPLHKCTYQNHHQQVKLLLEMGARVNAENHEGETPLHISVRKNSGECAHILAAWKGVDLNLANKYGETPLHYACSYGLIELAMLFLEKGAIPHLQDSQGDIPLMVSLRKGFTEISLTLIRWGVEVGDNGRGAPRSNSSSSLSISADNRSSHITDVGESPFVSPMLRPNNYNEHALHAAAMAGYSECVLALLGVGADINQFECYGNTPLHAASYTGNSDLVDMMITMGAIVNITNKDHVSPLHVAALAGKHRVVETLISRGGNCNLCDKNGDTPLHGACLSGDPQSVTTILTHGNISIDVKNAKQWTPLHMACASGHIVIVKLLVQMGANPNIKNISGDLPYDTAISSGHFDISTFLKKPKKSYKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2A1</accession>
    <name>A0A8J4V2A1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007358</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000387">
        <property type="protein sequence ID" value="KAF2071327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2A1"/>
    <dbReference type="OrthoDB" id="10536162at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="63"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="124" mass="13932" checksum="762D5554F961A6CF" modified="2022-05-25" version="1">MEPTSSYGNSNYNSGFNNPNQPFWARQTSPVVTDSTPLLVSDTDLEDRRHHKKKKGGCTAETFCVRLIMLLFFLGSAALAIIGLYHYSYVKKKQVDLTAVLWWVAAGIVLFVLFIGIIARKCRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2C6</accession>
    <name>A0A8J4V2C6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytoplasmic tRNA 2-thiolation protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_000470</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with NCS6/CTU1 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CTU2/NCS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000009">
        <property type="protein sequence ID" value="KAF2078180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2C6"/>
    <dbReference type="OrthoDB" id="25129at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03054">
        <property type="entry name" value="CTU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019407">
        <property type="entry name" value="CTU2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20882">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20882:SF14">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10288">
        <property type="entry name" value="CTU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="106"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2078180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="579" mass="64822" checksum="589E31224A50E8F7" modified="2022-05-25" version="1">MSLLDSIQSIVFNSKSINRVSINSAQCTKIDCHLSHSDGGNKSSAIGLLYVWYMSTHEEELPSCGSNDNDVNNTVPQSTRRSQLNLQDNTKCAKCVFNIINNTTKQKEKEKKEKKQQQKKKGSADDTAAAAAVTAVEEQPQVSEIIESAKDAIGKPIVHFRSESLCWECYKDLMLKKFRTNIVKARESKREAEKLLIALSGGTCSMVVAELLRQCIDGLGKSKMFFDIQCVHVDESLLYPYYQYQETLEYLRELTASFGFPFHVVPLENIYGTDPSTAESRRSRLIDSFKNVSSNTSKEDMIVYYREHLLSQTAHTLGCNKVVLGTSSNRLASKLVASTSKGRGFTVPNETSVVIEQPTNNIKFYQPLRDLLLKEIYIYYRHLRLREAPVIHPFTETKPKNSINTLCDDFLHCLQDISNQTVHTLLRSVDKLISPSVNSEFICSMCLSPLTSTEINAIESKKNSNNSSASSNSNECCSTNNSKSDQCCSTTSTSTTNGGCCSSDNNNNNSNNNSNNNLVQTLCYSCRILYGDIKQKESLAPYVKENSKTLLTTNQLKNEIKDFLLEDDQDEDEDEGETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2H8</accession>
    <name>A0A8J4V2H8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_009918</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000852">
        <property type="protein sequence ID" value="KAF2068759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10255522at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032432">
        <property type="term" value="C:actin filament bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051639">
        <property type="term" value="P:actin filament network formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21219">
        <property type="entry name" value="CH_PLS_FIM_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000010">
        <property type="entry name" value="Plastin-3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039959">
        <property type="entry name" value="Fimbrin/Plastin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961:SF31">
        <property type="entry name" value="ACTIN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961">
        <property type="entry name" value="FIMBRIN/PLASTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="6">
        <location>
            <begin position="986"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="6">
        <location>
            <begin position="1258"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="6">
        <location>
            <begin position="1374"/>
            <end position="1482"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="159"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="428"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="585"/>
            <end position="925"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2068759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1484" mass="170990" checksum="4D43F5EB06B180DF" modified="2022-05-25" version="1">MSKKKENNLINQLNTSIGSGGNALGVNSTQSSGGSFVKSSSTSTMSPFSIVSLKNSPPGSPMSLHRFNNRTPPLENFNIKEKIQHLGWMGSPQSVSSNGRLRSNSMAIGSPGSTTFLQHQLDQIGVKLEEKEKDAQLAAEIGKMLLERNQELEDQILILTESEKKYIEALSQYDFLKNQNERLNENLKEATITNEMLVDQIKEEEEEKGKKFLSAHRSNSVSNVKLQKDIDDLIMELDQMRQQFTQKEKAFNKLRIEKEDLLEQISTFEKEYSQINEYNAQIETYKEKIKYLSIENKSLKSFSELNIKKSIIEEFNHLKDNFQISLDKLSQISNSMIKEYSSSNEKQLYVNTKQLLESIQCDQPIVFQVINRIQELDGGTVDRLNCSKEFTDDNNSSPTLETAPTNLIRSSILLIISLYLISKSVEFSDKLVKEKNSTKELLEDLNELKDKFHTCSKLNDKLELDKLENGNEIQQLKVQLTNLESTLTSLKDNHEKELQDLSSEKEKLQELLKSQSENTEQTDLIFSLKETISTLSNEVNTLLNEKEKILKDYMDSQSQIIEKEKIISNFTSSHSQTEESLSLCRKDLELKNNLLKEKSEELDTKVKELDQLSQNSFVEKDNLKNLLLQKNNEIEQLNGNNQALVLEIEKLKGQLQDVENKSKQESVLLNETMEKYKFESNQSSQELINQHQIKIQEWVDKYQNLYNEFVGLKDAKCQQCLDLKSIIEELKNQIEELKTQLLNLQQLKYESETKLENQLTNNLEKQQSVEKELLENHESTLKEMKDLLMAENSNVIDHIKNQHQLQINSYIQDIEKLKDDNQSLKDAQLKFDTEFNRFQQSSLETSSNCKFLEKENETLLSEISIFKDQLSKIDMERDLFREKENNISNQIQVLKSQVDEKNLQNSNLQQETHSLKLELESLKSLLSIKDNQIQSLMDLNSNEQEKIIKKEEIIAKPPIKEDKKEIEPTSSLSTIEPHNCQEKPLVDREKKLFRYINSLFEANIPLDCQICAFRCFNNGILFCNIINHFIPETIDNRALYKGESSLENQRNIRISLNCIKVLGGDSSFSTQKIIDGNGQEIINLTYHILFVGLLHKINLQNHPELVYLWKFNPEFENKKKAESWDSFIAYEPQIYLRKWINHYLKVEGKPIVKDLCPNTIFSDYQILACILKNIYPSDYQSGQFKLDEKNSIYQFILNKIFKSKQDEMIVFSDDIESNDFQKCLILLSQIFDTQSGLSLSKDIDCPIIEPDSPDLGPSSEEKACKSWLKTTIDINTSNLDDFQDGLLLLKALDKIAPGIVNWKSVNMNPTNTFSMTENCNYCVTLGKQLKFSLVGIAGKDFVDGIKKFLLSFVWQMMRYSVLRRVNLQGSNGIEMSESELISKMNKLVQKNSKKSSMKSFSDPSLRDGIFLLDLLDSIHHGSINYKEVKYGDDIESRKSNAKYIISVARRLGCTAIIFWEDIVEVKPNMIMTFLCDLMTAPLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2I1</accession>
    <name>A0A8J4V2I1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ATP-grasp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_007124</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + H(+) = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + CO2</text>
            <dbReference type="Rhea" id="RHEA:10792"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="EC" id="4.1.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the AIR carboxylase family. Class I subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the C-terminal section; belongs to the AIR carboxylase family. Class II subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">In the N-terminal section; belongs to the SAICAR synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000363">
        <property type="protein sequence ID" value="KAF2071552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2I1"/>
    <dbReference type="OrthoDB" id="15425at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00130"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004638">
        <property type="term" value="F:phosphoribosylaminoimidazole carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004639">
        <property type="term" value="F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01416">
        <property type="entry name" value="SAICAR_synt_Ade5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000015">
        <property type="entry name" value="N5-carboxyaminoimidazole ribonucleotide synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000037">
        <property type="entry name" value="Phosphoribosylaminoimidazole carboxylase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000183">
        <property type="entry name" value="Probable multifunctional protein ADE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000020">
        <property type="entry name" value="Probable multifunctional protein ADE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01929">
        <property type="entry name" value="PurE_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01928">
        <property type="entry name" value="PurK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00137">
        <property type="entry name" value="SAICAR_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003135">
        <property type="entry name" value="ATP-grasp_carboxylate-amine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033747">
        <property type="entry name" value="PurE_ClassI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005875">
        <property type="entry name" value="PurK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040686">
        <property type="entry name" value="PurK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054350">
        <property type="entry name" value="PurT/PurK_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028923">
        <property type="entry name" value="SAICAR_synt/ADE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018236">
        <property type="entry name" value="SAICAR_synthetase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004676">
        <property type="entry name" value="PRK06019.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004679">
        <property type="entry name" value="PRK06019.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01162">
        <property type="entry name" value="purE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01161">
        <property type="entry name" value="purK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11609:SF5">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11609">
        <property type="entry name" value="PURINE BIOSYNTHESIS PROTEIN 6/7, PUR6/7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02222">
        <property type="entry name" value="ATP-grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17769">
        <property type="entry name" value="PurK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22660">
        <property type="entry name" value="RS_preATP-grasp-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01259">
        <property type="entry name" value="SAICAR_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52255">
        <property type="entry name" value="N5-CAIR mutase (phosphoribosylaminoimidazole carboxylase, PurE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01057">
        <property type="entry name" value="SAICAR_SYNTHETASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="16">
        <location>
            <begin position="680"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="276"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="526"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="284"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="538"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2071552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="950" mass="103984" checksum="0F43A85C399E0061" modified="2022-05-25" version="1">MSNIDTSDKTFLASGKTKTIYQLNKDDQHVLIVSNSAITAGDGAKKDILPNKDIYSNTTTCNVFKVFQCAGIKTHFVKQEQNNSFIAKKCSMIPLEVIVRRLATGSYLKRNTQVKEGTRFNPCLIEFTFKDDVQHDPLVTEQDILEMNLKIGGVSITSKVLSQIRHIANQAFEALEKAWQSVDVVLVDFKVEFGVTSTGEIILADVVDNDSWRIWPHGDKNQMKDKQVYRNIPLTGSTEGGEQKVNTLSQEQVKKIEENYAWVANQSAKLAEYASTHSNNTPTSSRLNVNNNNNTINSPHSSQQNITTSATTTSQSTSPIVGIIMGSQSDWETMKLAANTLTTLAVPFETRIVSAHRTPDRLFEYAKSAKSRGLKIIIAGAGGAAHLPGMVAALTPLPVFGVPVQSRTLSGVDSLLSIVQMPAGIPVGTVAIGAAGATNAALLAAAALAPWYPTIETNLDIYRKKQTDAVAECPVDGGAPTHQPTVHDSVSTFSSLLSAANSNNNNNNNNTVVTPLPQHIHPIITQQQQQNNQLVSPIRSQSPLPSGNGSNISSDKTPMSSFILSTTRPSALVLPPNSVIGILGGGQLARMIAIAAAQLGYKTHIFCPENDPSASHVSTFYTKANYNNYSALDTFAKQVNVVTYEFENIMVEPVEYLTKKLAVFPDPKILKTCQDRVMEKSFIQSLDIPTAKFEPIDSIESLREAVQKIGYPSILKSNTMGYDGKGQIKLSDDIDLEKAWKKVTSEVNVSQAILEQYIEFESEASIIVARGLDNTEVCFPLVTNKHRNHILRQTIAPAQLPEYVHKDAKEIATKIAKSIGLVGVLAVELFVVRDTATDTYKLLVNELAPRPHNSGHWTIEGCVTSQFEQLVRCVCGLPLGSVDFMRSISEKEYLKQNLQPIVMTNLLGNEVDQWEKQLSTKGSHLHIYAKGDARDGRKMGHFTLIPSEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2L0</accession>
    <name>A0A8J4V2L0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_007032</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000353">
        <property type="protein sequence ID" value="KAF2071657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2L0"/>
    <dbReference type="OrthoDB" id="278280at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032378">
        <property type="entry name" value="ZC3H15/TMA46_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12681:SF0">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12681">
        <property type="entry name" value="ZINC FINGER-CONTAINING PROTEIN P48ZNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16543">
        <property type="entry name" value="DFRP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="96"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="173"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="96"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="173"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="334"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="338"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2071657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="382" mass="43758" checksum="7377FE7E5DFCBF77" modified="2022-05-25" version="1">MPPKPTASKKTVDKDKKKKIEDKTFGLKNKNKSKKVADYVKTVEQQVQNSSLQNKNNAKAAAAKKEKEAAEAAKKEMEAGLGKLTIVQPKLALGVDPKSVVCEFFRHGQCQKGNRCKFSHDLQVERKSVKIDIYTDRRKGETDDKKNDNMEDWDDEKLKTVVDNKRSAANKKNQTDIICKFFLDAIESKKYGWFWECPNGGEKCAYKHCLPEGYQLKKKKTKGEEEEEEIPIEELIEEERAKLTKHTPVTLETFLKWKEEKKLQKEKAEKAASDKRAADIKAGKTSMSGREMFEFNSDLFVDDESAIDVKSKEYERTEEEKQADLVLVENIQKSLFLDGEDDIPDMSDEDEDEDEDEEEENEDSDGGEVQPEDEEEDEASDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2T6</accession>
    <name>A0A8J4V2T6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002450</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000067">
        <property type="protein sequence ID" value="KAF2076273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2T6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140860">
        <property type="entry name" value="Pseudo ankyrin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="801" mass="94978" checksum="D039C6602E93796F" modified="2022-05-25" version="1">MNELFQKIYKNVFLKNKIFGYLGSQRKGYYLYSNSVIYHQYDDLQLHDIINTRNKVILLEKLNRYKSLFNNSNSNNSNSDNNRERSFTYYQDWLDYNSNSNKKKIDIEGLKGIFKDDNEIDSFLNDLYQLFLNENRNSLEYFPDLVFRTFGYTNKRRKIMGFNKTFSTHSKSGTLNTDLTRLFDTRYGALKNIQFRCYSLFIDDPFFLENKDRFRSLDFWNDNDFVYGLLKDYCTCCNSINSNGNNNNNNKLELFKWILSVSSFFNYDLYVQKYGSKGVSFTMMFGYKVVVYKDVEEMEQVPFIKSGFFLLEVFQYLSSFYPNLFNESVILELVMHQMIKIEDYQIAKAFVEYWPKQGGKFEHYPLESIFVISTHSSDMRLFELLRTHFPQYQVRNILSTRANIEHFLLHNPGIFEYYDLFSTTLPSDVDPFEIYKMIKPANTMSNNIILEAVKRGRIDFLEHCIDRIVTSLPLTGGKKTFMMCAYSYGHWNILDYLYKLWYSGANNGGNTLESDEMYKSLLYQSMSKYDLHNSIVFYNSIKDVNYQRQTTSALIVDVYMLLSSTTSRKEKDIVIEMVKFLKDACKQHNIHQLDSTNKPMVLQDIEYIRQQQFIVFRNTPSILDLFRESMVLGELNETKLALSLMTNDQHQRLTGSITSLIIAYLNQHRKPKLKVILYLFDRFQSTLAPHTFDLLAKICYIAMEFKNHHLIDILLHQTKIRLFKPVSPQHDHEMLCHNHHYLSVAPFKCDHFKNLVKRLHINTNKDAYQNKNTIVPDKDVILDFNITHQHNSFNKRQNYYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V370</accession>
    <name>A0A8J4V370_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000056">
        <property type="protein sequence ID" value="KAF2076573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V370"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="167" mass="19770" checksum="EF19482879D73023" modified="2022-05-25" version="1">MDVYEETGIEGNEKEEEHHQQNQLNQQNQQGQQGQQEVEEEEEEEVVTYTTYSEFSLAISTFFEQLLEIIVSRISDFPSIRDLIEILSILTPDDFINFSNSTKDENFCFVSFLVSRNIKMDPIFIDTILRLNFHNVWIFVEDGIVEYDVPIEFKSYGDKLLQYLRKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3D1</accession>
    <name>A0A8J4V3D1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006222</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000277">
        <property type="protein sequence ID" value="KAF2072467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3D1"/>
    <dbReference type="OrthoDB" id="16955at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008614">
        <property type="entry name" value="FIBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13223">
        <property type="entry name" value="ACIDIC FIBROBLAST GROWTH FACTOR INTRACELLULAR BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13223:SF2">
        <property type="entry name" value="ACIDIC FIBROBLAST GROWTH FACTOR INTRACELLULAR-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05427">
        <property type="entry name" value="FIBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="309"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="459" mass="53231" checksum="8C8CFBA92B4E5B0F" modified="2022-05-25" version="1">MMEMKSKVLFDVFVSDPLSVNKFIYRSWLDGYTEKEAANMREDTRLVGAPRNLILQETEDQYRNFTLLQKALEHPKTLSSHSMFQMDASSRRLLIEGYYDFDEVLMRELIGRKLTSGQRRDLDDASEKLKIRQSSCERQFDNLKRISRLVFSDLKTSALDIIMNEFSLPRELSKKYAWLLFLCFHRVDLSKKRIQFLNTFDLIRLSEIIMNHWGDPLKIYTIELDIKLKEDVRDLKTHLNSQKEISEFYMRLIKTRYQSVNSSPRILPSSSSSISSVGIVDYSSTTPNLQSNQPPISGFVPPMFSQQGTSSNINASNNTNNLQQQQQQQQQQQQQTPIFTSSSAIVVPPNTYPLSNEKLKALESKFRSILKGILAIGSNLSDSKEFRNLFEDIVEKVIDPLKRIDLSIGEADTFFIHLREVFNPEQLNLTLRHRDRVVHNWKSFLEGIRGVVLYIYSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4P2</accession>
    <name>A0A8J4V4P2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="8">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_004944</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">Belongs to the peptidase C12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="8"/>
    <dbReference type="EMBL" id="AJWJ01000185">
        <property type="protein sequence ID" value="KAF2073742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4P2"/>
    <dbReference type="OrthoDB" id="427186at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09616">
        <property type="entry name" value="Peptidase_C12_UCH_L1_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.532.10:FF:000006">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.532.10">
        <property type="entry name" value="Peptidase C12, ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001578">
        <property type="entry name" value="Peptidase_C12_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036959">
        <property type="entry name" value="Peptidase_C12_UCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589:SF17">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01088">
        <property type="entry name" value="Peptidase_C12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00707">
        <property type="entry name" value="UBCTHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52048">
        <property type="entry name" value="UCH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7 8" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 7 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UCH catalytic" evidence="10">
        <location>
            <begin position="11"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="233"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="248"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <feature type="site" description="Important for enzyme activity" evidence="7">
        <location>
            <position position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2073742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="257" mass="29223" checksum="73D4089AFD69AE8D" modified="2022-05-25" version="1">MDESLLETQKTWIPLEANPEVLTTFMQSLGVAKEWEFCDIYGIDQELLDMVQKPIAAVILLFPITDAYEQNRYKLEDEITEKGQILSDKVYFMRQYIGNACGTIGVIHSVLNNSDVISFEPNGFFSKFLATTTTLNGEERAISLMKNSDIEKSHEIAAVQGQSTVPNEDEPVILHFVSFVHVDGHLYELDGRKPFPINHGESSQETILEDTAEVLRKMIEVNPDEIRFTLMGLVKKSEEEEEEEEEKEKEKEGAKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5R7</accession>
    <name>A0A8J4V5R7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Peptidase M8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_003698</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000119">
        <property type="protein sequence ID" value="KAF2075002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5R7"/>
    <dbReference type="OrthoDB" id="527990at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.132.10:FF:000001">
        <property type="entry name" value="leishmanolysin-like peptidase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.132.10">
        <property type="entry name" value="Leishmanolysin , domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.55.10">
        <property type="entry name" value="Leishmanolysin domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001577">
        <property type="entry name" value="Peptidase_M8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10942">
        <property type="entry name" value="LEISHMANOLYSIN-LIKE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10942:SF46">
        <property type="entry name" value="LEISHMANOLYSIN-LIKE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01457">
        <property type="entry name" value="Peptidase_M8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035187291" description="Peptidase M8" evidence="9">
        <location>
            <begin position="21"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="314"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="403"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601577-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601577-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2075002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="661" mass="75519" checksum="54B7AD9A9ED9420A" modified="2022-05-25" version="1">MRYLFNLFICLFLFSSLCHGIKVNDIIDSYYKEFSTQIESTNQQQQLYHNGLQQNKRARAIYNPPTSKKSCIHDEMVLDKFIKKDVDNMGPKKSNKLNIPLAEEYVKDDVGTPIKFFANSTYIDNDELSCRKVGQKVIFSKSTETTTRDCVLDDYGVVTNSPCKYTCTENDILTPELQNLIKNFVVNATIDIFQSLLRVRNHKSNLKLIHEVGEKRPVQCSKGVFIPESLYIDGIDNVDHYLFITSRPTPDPNTIAYSIICDAPVLFYGEKDEDWVYGRPQVSVLNFNPSYFLDVIRKKSKWDFNKYLRVGIHEMVHSLGFSSFLYESFIDPETGAPYYFSGQSVVVDLKIPGMSPAGSRFLRQRSMITSPSVVEYSKKHFGCSDAIGFELEDSGGSGTKGSHWEKRTGAEEIMTGYISELLPVTNLTLSLLYDTGWYIPNFAFGEEIGWGKNMGCDWMNDCNPSSWNQLGYFCEKRGQLGCTANRDGKGVCQVKHYSASIPGIYQHFNSTTFGGSDKSYDYCPFYQVRPAINRHTSYCANFEFPSDESINEEYGEGSKCFEYEFKNYPTSDHYYDSDDSDDNNSYDQLNLACWVHRCNAKNRSLEIKINNFWYTCSGSEFIDVLVPKGTISIKCPYDMCPYEGFKDYNTDKSTNTPNPLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V614</accession>
    <name>A0A8J4V614_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pleckstrin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_006339</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000288">
        <property type="protein sequence ID" value="KAF2072344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V614"/>
    <dbReference type="OrthoDB" id="20783at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00139">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF8">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 5-PHOSPHATE 4-KINASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="261"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="PIPK" evidence="4">
        <location>
            <begin position="414"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="84"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="216"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="84"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="131"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="221"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2072344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="763" mass="85507" checksum="45E24ED28416ACA5" modified="2022-05-25" version="1">MSHDGDGENRLVIDIINTTTTDDVDNGNGNGADTTTEAAEIPLSESTTTSTSTTTTVIESSSTLETTFEEISLTSVTITTPSTMVEATTSSNSNNNIPKVSKWRESQPYKAQSQRQLIDSPRVISENGSITLSNSASSENLNSSTSSLSSNTTSTSLNNSSNSINLSTSSSSTTTTGVTTTTTIGTGTTNLSTSNSSTSSTTSSFWSSISGWGSKLRSKDNQNNSSNNQNSNISTYEINNNDQDDGEDTICNNSTIYPGSQIIREGFLNKQGHLRKNWLCRYFILTDTSLEYFKDKGQPLINRMPLSECTLSMADVELVKRPLCFKIVHTPSKRSFYIYSNEKSNSQEYEQDSYQWIGAIQEVIDTFNHRVLEQQSNKESAQVNTSASLVNEDHKNHELVSCIIHALINCIGRISATVPTDISLSDFQAYEVKESCTPATMTPASSTPTNSPPPNVIIHTPATSTPPKTTQYTYKFIDYAPKIFRKIRELSNVNSEDYMLSLTKNTLTEEPTQGRSNSLFYFTEDKKLLIKTITNTEFEQLRNVLTNYYFHLLQNPDSLVVRFYGLHYISPSKSKNTYFVVMENIFTDKSMDEIYDLKGSTLHRKKEQGKKVGMDLDFCQRILVSEDFKQKFLKQIESDCNFLEKNSCMDYSLLLGIKKISKDDVENYNREKENQQQQPVSVFKKDMGGIFATTCSGEIYDRVYYIAIIDILTVYNIKKQIEHTYKSVAYDTADSMSAVDPQTYNKRFKTFINKVTGYPKIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6M7</accession>
    <name>A0A8J4V6M7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pesticidal crystal protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005665</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000232">
        <property type="protein sequence ID" value="KAF2073019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6M7"/>
    <dbReference type="OrthoDB" id="23795at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.10">
        <property type="entry name" value="Pesticidal crystal protein, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036716">
        <property type="entry name" value="Pest_crys_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35598">
        <property type="entry name" value="ENDOTOXIN_N DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35598:SF2">
        <property type="entry name" value="PESTICIDAL CRYSTAL PROTEIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56849">
        <property type="entry name" value="delta-Endotoxin (insectocide), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="482" mass="55652" checksum="62E6500920E4E632" modified="2022-05-25" version="1">MSQPDLTVIEKFNPGFAIGRAVLGEEYKLDNDDYLELIKGTIGCIPVVGALATSVFSIFWKKAFPVEDPYLRKADFEKRMKKLITDMENKMDKKIDDEIVKFCQSKFESLCSAGFGFSEIEYIYDKQKREGKPTSDSVKMYMAANHVSFRNLLLECLIFFSREDKIDLLCKLYLQTVVMYIAFLRDTHFHGLDWGLPEEYVNGVDHIKSMEYKTHETIELAHSYYSKFFKKLNPPRDYTYEREVMIMEENGLTHMGYLPTKFKCDYRPLLAQMTYADLTRYNLNQIWLQPTTKEGKVREKKSGNGIFGGMDPQFFMDSESYHYRLPVSNRIYIADFSKMPSMRGSYKQTEDGKNSVNSVTGTVGMTSNTSNGIFYKCFYFTEPKNLIFRVLANIDSNAQIKMIIREIPEGEDAENPDRSSQVATKSHSENIAYKKLFESSSGTMAEFVSKQYKFEKPGSIYFQAEVVGGNTRNLYTLEIIDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6R2</accession>
    <name>A0A8J4V6R2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA mismatch repair proteins mutS family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_005565</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the post-replicative DNA mismatch repair system (MMR).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000225">
        <property type="protein sequence ID" value="KAF2073124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6R2"/>
    <dbReference type="OrthoDB" id="10252754at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043504">
        <property type="term" value="P:mitochondrial DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001238">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005748">
        <property type="entry name" value="DNA_mismatch_repair_MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01070">
        <property type="entry name" value="mutS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003810">
        <property type="entry name" value="PRK05399.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF34">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="DNA repair protein MutS, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="9">
        <location>
            <begin position="895"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="125"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="151"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2073124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1009" mass="113546" checksum="FBB07C9F54A4D31A" modified="2022-05-25" version="1">MLLVKRSIILNKNNNNISQCSSLISYFTNSYNNSRFYSTGDNNEQQQKKVVRIVSSKKSVSSVSSVPSFISLRNNSNSSNSSSTLKETTTTTTSVVTNKISKETIDHFVPLNNIDLSSSPSIFSTDVKKKTRKSKGKQRGAEQDESSVKINDQEEHREKEQQQKESSINTYYYNQDSERIDLIDKGKDTPIIKQYKEFKSKHPNSILLFRLGDFYEMLFQDAIVASKILGIALTCKGGRADTPMCGVPSHSVDVHLQKLIKSGQTIAICDQVEDAMTAKLRGGIITRQVTRVITPGTLFEDNLLNNSQNNFLCNFLPIDMDFSALDNAYTFKNERFSISWLDISTGHFFISETTFDSLPSELSRISPNEILLPRSLIVHPQFGKLLKQYHVTTDYNDSFYFANTQSKFTQTFGDDIDLLATFSQQQLNSSGSILNYIFKTQIGQIPHLYLPQLFNNQQSMFIDYSTMSSLEITKTYSGHRKGTLLDSIDKTLTSPGSRLLYSRIQSPSLNYNEIKSRLNMVEFFYNNTGLVYEIRKLLTKCLDLERCLQRVYIGKAGPKDLSAIGATLAFCCQISQSLSRVLRNEKSIDPVLSQCLAKLDESKYEELLDELSAALVDNPPFSTSDGGFIEKGYSKKLDQLLLFKDHSREMVQSITDKHRVHLNFPALKIKHNQTQGYYFEILSQQRDKIPSDFIHVQTLSSHLRYKSKELNEVQEKINIAAFESLQEEQSIFNDLCKLVLKFGEQIKEASQALAIIDVSSSLGLLSKERVYTRPTIVLDNVLHIVNGRHPTVELSMINGGTFIGNDCNVGMGKDKLWLITGPNMGGKSTFLRQNALIILMAQMGCFVPADYAEIGITDAIFSRVGSSDDLSNDKSTFMVEMIETAAILRKSTPKSFVIMDEVGRGTSTLDGLSIAQSVVEHLVQENKCRTLFATHYHELTNTFTGNESIKNYALAVQEQDDEILFTHKILPGVANKSYGIFCAKLAGLPDRVIQRSQSILSNFENNKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7S1</accession>
    <name>A0A8J4V7S1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription factor CBF/NF-Y/archaeal histone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004438</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the histone H2B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000156">
        <property type="protein sequence ID" value="KAF2074264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7S1"/>
    <dbReference type="OrthoDB" id="30550at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="2">
        <location>
            <begin position="25"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="206" mass="23123" checksum="7FB1FF94D32CB34A" modified="2022-05-25" version="1">MVRRGIQQYTHRGYHAKHPREGAFFKKLLKQISPNSIRLAVTSSHVLTSLLREFSKDIINTALEIESASKKRTLSAKSIQTAVRLCLNGELSRHAVASGVQAVSQAKTDKKPAIPISYVRNQIKSSLTILRISKSACFYLSAVLEYLLSEILELSINRATNDKKRLITPRFIFLSICCDEELNKTFNKPGTIFVQGGPYVFGNSYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7Y0</accession>
    <name>A0A8J4V7Y0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001456</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000034">
        <property type="protein sequence ID" value="KAF2077267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7Y0"/>
    <dbReference type="OrthoDB" id="6019893at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901981">
        <property type="term" value="F:phosphatidylinositol phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040032">
        <property type="entry name" value="DENND1A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005113">
        <property type="entry name" value="uDENN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13196">
        <property type="entry name" value="DENN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13196:SF14">
        <property type="entry name" value="UDENN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03456">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00800">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="2">
        <location>
            <begin position="110"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="333"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="661" mass="74593" checksum="F5D14016CD63EECA" modified="2022-05-25" version="1">MMSIPFFKKEHKRTPSGRRQLDITIDVETLTQDEKLSRSLHTGVRKRSVIDRPRLFEWFLVIGVPPIQSTVNSPLNSSVGSSPITSPPLQQPPTLSTKPTLLRSSSPTPQSPHSASPLLNGGTAAIAQPKVLYQYPPDKPLDNLSVEFFPFPQGVPITNVSQTASHTNLFQMLYPQRHLKNLEDSFVFMLTDEKKDVLYGVCTNKVSSIGSLLAGEKYEIDIDENPNQDDYIHVGEMVPTAPKCYCILSKYPFFPIHFNIINGVLSLQHIQEITRFQNIMSSSKKSKPNAIQTTSTSTPLSMSKSMTNSLERKIEPNGNGKDKDKDKGTPDSNNNNNNNIIPTIEITNGSSLQTSSTSNILHEEKPTPPRKYPILECIEFFYSQNIPMPSQTVQYDFPGLESIIKFKRGPCKTKDDFTSDYDDYMLEYGLFTTVQLLTHKTLIVILSAILLEKRVVFYSKTIRSLTSVIFSFISLIKPFNYQSVILPLLPSSVSSLSIQDLLSAPVPYIIGLTGDLPKDVDFSDIVLVDIDQNKIQSKVVQLLPRWQELANKLMHIVKELKPSMTKQLAFVATAEQEVILNRITIAVETHISSLFDNFSRHCIRNVTDKQKISIFVKESFLIAEYPDAEQSDWINEFMKTLIFSVFLDKKLRTDDNDLVFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V994</accession>
    <name>A0A8J4V994_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dynein light chain roadblock</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_000152</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
            <location evidence="1 10">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the GAMAD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000003">
        <property type="protein sequence ID" value="KAF2078527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V994"/>
    <dbReference type="OrthoDB" id="9985637at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.30:FF:000011">
        <property type="entry name" value="Dynein light chain roadblock"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016561">
        <property type="entry name" value="DYNLRB1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10779">
        <property type="entry name" value="DYNEIN LIGHT CHAIN ROADBLOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03259">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009998">
        <property type="entry name" value="DLC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00960">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103196">
        <property type="entry name" value="Roadblock/LC7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6 10" id="KW-0243">Dynein</keyword>
    <keyword evidence="5 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Roadblock/LAMTOR2" evidence="11">
        <location>
            <begin position="4"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR009998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2078527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="97" mass="11193" checksum="056A1022F569803B" modified="2022-05-25" version="1">MSEIEESFKRIQTHKGVVGVLIINRNGSVIKSTFDQETSVSYSKMVIDIFPKADQLLKINSDNDELSFFRVRSKDNDIMITPDKDFFLLVVNKIHKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VAL6</accession>
    <name>A0A8J4VAL6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_001404</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000033">
        <property type="protein sequence ID" value="KAF2077279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VAL6"/>
    <dbReference type="OrthoDB" id="18853at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="5">
        <location>
            <begin position="22"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="5">
        <location>
            <begin position="155"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="415"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="291"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="146"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="415"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2077279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="445" mass="50400" checksum="F89AE8DAE91CBD1E" modified="2022-05-25" version="1">MSTSDIGGSTSATALAAKKWEDAQEKAFTNWVNTLLSKKGEKVENIATDFADGVKLIHFLELISGKQCKRKYNSDPKDKINKIQNLHIALSFLYDDLQLKSRSAGAEDFYDGNRKMIFGFLWTLYRKFRISSIKGPSQNNNGNSSNEREKDPSEREAEDNLLKWCSNMTGMNVTQFKSGFRDGMAFLEMAKSFSDPQDSSLLDSFGGAGVDSIARLNAVFDFAEKNLNIPKILDAEDLVSGAVDERTVMLYTSMFYNAYEGRKKQSKSETEELVEKVMEVSDVQRETIEKIFRLEETLNMYNEELRLQRLERMALEKMVKQQENDSFLSNLLLLRDSLEDHQIKLCKLQQRVQEAATGKLAPLWGPSREVIDLSLTEYQQIEMLGELLEDETNRINRIVQVTEVKPIAFIKKPTSTTTTTTTTTTTTTTTTTNSSNVKDDGSDNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VBX2</accession>
    <name>A0A8J4VBX2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000129</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000002">
        <property type="protein sequence ID" value="KAF2078629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VBX2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2078629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="71" mass="8218" checksum="7A1A8C7A41920172" modified="2022-05-25" version="1" fragment="single">MPKKEHVDFAINNVWPEICKFLPQGETDAEKIEYYKEYNQKVAKAVIFSILPVYMHSDASKLKDAKEQKGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJY0</accession>
    <name>A0A8J4PJY0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_009640</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000764">
        <property type="protein sequence ID" value="KAF2069042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJY0"/>
    <dbReference type="OrthoDB" id="21578at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053370">
        <property type="entry name" value="QS_Complex_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35035">
        <property type="entry name" value="DISCOIDIN-INDUCING COMPLEX SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35035:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035303228" description="Transmembrane protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="902"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="838"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="844"/>
            <end position="870"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2069042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="956" mass="103661" checksum="7CA2CAE2967F241E" modified="2022-05-25" version="1">MKFLILLLFLFSFWGGFLNAGQVVWDGTTGNWETPTNWDTDTLPGAADIVFMIKNNSVITSNAAHQVDIVIVGYQVDYPVVKFIANKGLTCSEFQIKPNATAIFNLPLPTDTFSGDIFVEGTFETNLFFNSNSFITIKSGLNSKYQNFAKSKYYGIDNYQTFYSNNDITVTDVWQNRALMNILNGVIDIQPNSLYMNDSFTFFKGATVNSKSFVTKVYQTAGLTIRDSTYKINNINYDENDEDSIGVFRSQDSAQVSIENSKLLLNNTNYYNLNDSLSNFENSQITLNQAFLNEQRAVAQFSNTTLFTTHVFQLDDDTQVFLRKNSSLTIQDKHLGMYLNSFMELRDSNLTINSINTDLSILGLFNDSTLAIVENSKFSLDGHFLVCNTSTLIVSNSSYVSIRNILQGTEDSYIKVVNSSTIEIFGNVILFETSTFTAIDSNVIVHGYFHLWDSSWFVFRKAKVNIIGRLQYVNEEPLYYSQMDDSEVDVNGVLLNAGNLKINNTLLDVQLNFTTYGTFEAQNSRIIVRAGIFYTKGKLSSYGTNFTVHNGLASVGDDSQFFCEKCIIEINQGNLKQGRNSNVTLLSTVLKNNNGIVTSLSDYIVNPNSLISNNATFILTTNVLAVLDNNNKTDSFIENTGNFEISGGTTTEINIPFKNNGGILNVQQNSTFISLHQNNGTLGLNGGTVSSNSTIEISGGSVSGNGEIGGNVNNTGGSIGGSDTNHIKINGNYTQSDNGQIVITINDEQDLTTLNISGNANIENSTIVIRVNEMITVEKNATVVSSANIVGTFGSIVFKTFSPTSGQETTTKTCVHSSSTNNVGFSVLLNQKDKVKCTTSTGGSNTGGSNTGGSNTGGSNTGGSDTGGSISGISGNGSYQVDNGNSDVSIFSGSSSLSKGTVIGVVVGVVGAVAIVGGIIYFRNRIPTGQQLRNQLKNLSPSRRGSKGPSVKLGQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKX7</accession>
    <name>A0A8J4PKX7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008874</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000592">
        <property type="protein sequence ID" value="KAF2069805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKX7"/>
    <dbReference type="OrthoDB" id="22869at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38082">
        <property type="entry name" value="ENDOTOXIN_N DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38082:SF2">
        <property type="entry name" value="PESTICIDAL CRYSTAL PROTEIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="303" mass="34606" checksum="931F7F41F6334CF0" modified="2022-05-25" version="1">MQTILLQCVSTIQLCSFWYQIDLDKYYILGVKKEKEGDVEVKSIKQEYHDLLVECLKNITPAVHKNLVDEKPELNKYYNNNASKMFNTNPWIYPIPLVIAPLNVPRVATDFHLVSSTPTINIPLERKTPFIYRIDGANMLPEQRIEATSGYRLIPEIDPLTKCKGTLIHHYNSINISLPERNNVTIRIFGIYPKSISKLSFVKDKTLQEQECVEESMFCLPPSFKSNSKSFKACKFDKMLIGRFAEEFYLGPNGAISGFTYLKPYMNVNTISFNVCQVSKGPDHASKRGCIKFIELIISDVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL14</accession>
    <name>A0A8J4PL14_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thiamin pyrophosphokinase thiamin-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_008802</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000580">
        <property type="protein sequence ID" value="KAF2069880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL14"/>
    <dbReference type="OrthoDB" id="25149at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030975">
        <property type="term" value="F:thiamine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004788">
        <property type="term" value="F:thiamine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006772">
        <property type="term" value="P:thiamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07995">
        <property type="entry name" value="TPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.320:FF:000001">
        <property type="entry name" value="Thiamine pyrophosphokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10240">
        <property type="entry name" value="Thiamin pyrophosphokinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.320">
        <property type="entry name" value="Thiamin pyrophosphokinase, thiamin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006282">
        <property type="entry name" value="Thi_PPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007373">
        <property type="entry name" value="Thiamin_PyroPKinase_B1-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036371">
        <property type="entry name" value="TPK_B1-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007371">
        <property type="entry name" value="TPK_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036759">
        <property type="entry name" value="TPK_catalytic_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01378">
        <property type="entry name" value="thi_PPkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622:SF8">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04265">
        <property type="entry name" value="TPK_B1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04263">
        <property type="entry name" value="TPK_catalytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00983">
        <property type="entry name" value="TPK_B1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63999">
        <property type="entry name" value="Thiamin pyrophosphokinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63862">
        <property type="entry name" value="Thiamin pyrophosphokinase, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiamin pyrophosphokinase thiamin-binding" evidence="6">
        <location>
            <begin position="335"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="127"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="138"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="151"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2069880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="409" mass="45166" checksum="45B2E81ECFE543B8" modified="2022-05-25" version="1">MSETLSPSSSPATNTTSSPSNEEKPIKKWYCDEYGAYIYRDLEDTDEGSDVGDVDSSIELISLTTRDSSATTTQEYSDMLSGQMTSSSATVHKTLSSLTTITLESPESSHSTPLSYTPSIESPKKLEQLEQGESAQEQQHKDDSLEKDNQETTPATTTPSSETPKRSLGSSTLSLKRSQSLTDQNVLIIINNSISMLHIQYLWSKCSVKICADGGANKLFNMTQNRKDLEKWIPDYIKGDLDSLKDDVSEFYSKKGTTIIQDTSQDTTDLQKCMELVSELEENTGVRYNNIFIAGGLGGNTSHEMANLNTLFLYKDRKMILVSNTNVSWLLDSSQQHEIDCRVETKCSLLPLGQPVEKATTNGLKWNLVKQSLNFGGLVSTSNLSIDTKITVDASHPILFIVDTNDINN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL40</accession>
    <name>A0A8J4PL40_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010552</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001190">
        <property type="protein sequence ID" value="KAF2068121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL40"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="87" mass="10126" checksum="A0E96056E81BC0B9" modified="2022-05-25" version="1" fragment="single">KPLPKLILKPLYAKQPPQKPPQDPQQQKQQQNLQQQPQDSQQPQPPRNPPQQQPQPPQSPQQHHSKGRSKKLYDKLQGYFQNPVKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL44</accession>
    <name>A0A8J4PL44_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009766</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000800">
        <property type="protein sequence ID" value="KAF2068913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL44"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="97" mass="11436" checksum="C0707EA295EDC5FE" modified="2022-05-25" version="1">MSEPNQNKNLNLWQRWKLIEPRKRIVISFTVLVFSSLSLFFEPKEGESNIMNHSQRPGVTIKTIDYSKPADKRVSITRDGVDVTKEELEKNQNKKWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLB5</accession>
    <name>A0A8J4PLB5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_008617</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000549">
        <property type="protein sequence ID" value="KAF2070060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLB5"/>
    <dbReference type="OrthoDB" id="1716625at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09913">
        <property type="entry name" value="EHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000147">
        <property type="entry name" value="EH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040990">
        <property type="entry name" value="DUF5600"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031692">
        <property type="entry name" value="EHD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF31">
        <property type="entry name" value="AT21416P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18150">
        <property type="entry name" value="DUF5600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16880">
        <property type="entry name" value="EHD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="14">
        <location>
            <begin position="53"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="12">
        <location>
            <begin position="430"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="462"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="517"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2070060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="573" mass="64837" checksum="E88F3AD9C20A899C" modified="2022-05-25" version="1">MKKLNVSENKEQERIYTSSIDALKSLYSSKIKPLEQSTKYGDFFSPSLSDSDIEAKPMVLLLGQYSTGKTTFIQYLVERDFPGQNIGPEPTTDRFNALMYGNEDRIIPGNTACVQEDKPFKALSRFGTGFMNKFQCSQCNAPILEKLTFIDTPGVLSGEKQRIGRAYDFPAIVSWFAERSDMILLLFDAHKLDISDEFKSAIECLKGHDDKIKIVLNKADKISAQQLLRVYGALMWSLGKVVKTPEVMRVYLGSFWSGGPLQNPETESLLHSEMVDLMKELLVLPKNAAIRKVNDLVKRARSAKVHAIILSHLRNEMPSVFGKDSKQKELIDNLDKEFYKIQRMYNLPPSDFPDLQRYREQLKLYDFSKFPKINTRMLQTLDEVLSVDFPTLLQKFPLDGSHVPTQYELNPFAIDEVDENIRWTLFEYIDKNSLSAIFATLNPIDGKISGAAAKGPLQQSGLQNDILAKIWRLSDVDKDGKLDLDEFCLAIHLTQCRAKGIEIPDILPPTLIPHSKRGGMLESDFNPSKTIEGAKMKRPSSKNLLKKSGEKSFFKKNNTIDNLKSSTSSTDMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLE0</accession>
    <name>A0A8J4PLE0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Myb domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_008579</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000542">
        <property type="protein sequence ID" value="KAF2070105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLE0"/>
    <dbReference type="OrthoDB" id="118550at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44042">
        <property type="entry name" value="DUPLICATED HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44042:SF67">
        <property type="entry name" value="MYB-LIKE PROTEIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="106"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="6">
        <location>
            <begin position="109"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="192"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="313"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="515"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="580"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="68"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="168"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="193"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="212"/>
            <end position="224"/>
        </location>
    </feature>
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        <location>
            <begin position="313"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="326"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="526"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="539"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="589"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2070105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="607" mass="68986" checksum="B4F6E418654FB3E3" modified="2022-05-25" version="1">MNPNQIIRYPDPYDFGSPSSPSPPPMYSPLSYRHPMFPMHYPGRYIDSQHEMNNGDESPDKSYQSNTIIDLQRRVQFLEEEISRKRDTIEVLQLQVNKYEQEKQAEKKKQSRYWTPEEHARFLEALKKYGHKDVKAISLHVATRNATQVRTHAQKYFLRIDRERGRKLEHKDSDKNENDDWSQDDYVDDGSPLYSSCTNSPTTHPQNPFPSPTSSSVVPSISPSPKRKREAVTITASQAKSAMLYKDAVLSVLPCSWTQHDYDQFSKGLISHINEDDIRTLCKLIREHFLEQQPLDIIETAYYAFLKAVQQKSSKDSNGNSSPTSNNNNNNNLNNNNNNNNNNNNNNNNNSNNNQNNNQNNNNNNNNNNNNNNNNNNNNNNNNQQQNQQQIQQQQNQQQQYNQQQQQQNINRTSPTLKKRPAPVNVTRPEFEVTPLSTPPTPSPSYQSPHYPVQAYNGNQSPMGMIPNPNLLNHHPPSPNLSSPTTHPIRWPGPLVPFEHFRRLETHYGGQPAYVSLQPHGVANGYDQSPISPTEESNHINSPSSSSSSSSNSSWLPPPYQPVESPNSWAMNQTISAFSNTSLSMNNTPPNNLNSHLIPSSSNSSND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLT2</accession>
    <name>A0A8J4PLT2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010165</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000953">
        <property type="protein sequence ID" value="KAF2068511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLT2"/>
    <dbReference type="OrthoDB" id="23220at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035159577" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="27"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="17104" checksum="BCF69330A875690C" modified="2022-05-25" version="1">MKFLYFVLAFVAFVTIFNSMAGVADAHSAAGKGNITYCTMCNTSANCDPKSNHSCSELVLGVCQKINNKCQGTFSYGILTQTNQTISTTIYNDDACKSLNDTTKVPEKNCFTCYNDLQILCYESHEDSHGNSASNVLPGALFTLMASLLAILFKFIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLX5</accession>
    <name>A0A8J4PLX5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000494">
        <property type="protein sequence ID" value="KAF2070420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLX5"/>
    <dbReference type="OrthoDB" id="21545at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="338"/>
        </location>
    </feature>
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        <location>
            <begin position="377"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="543"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="935"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1174"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="129"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="152"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="199"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="261"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="296"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="400"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="424"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="455"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="949"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="964"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="992"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1025"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1040"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1060"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1104"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1129"/>
            <end position="1152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1237" mass="132424" checksum="CB405EA41F6BE5D7" modified="2022-05-25" version="1">MDHSNNNSGASSTTPNNSSNNNSGSSSTMSSSPSSTTSSPSSSSTSSPSSASAKPPPPPPQNKPAIATTPAATVVNSTSKPAATIIKTYPPPSSGPANNNGGPANTTPPPQPTIGTIAKVVVKSVPSATVPTTTTTNNTPTTSPTIGPRKIVTTNSSLATTSSPPTATTTTTSTVASAPGSVLKPIIITDSSPTPPPTTATYKAPATTTATTGSPTTSPTNTPIKTSSGSINVSIQPKPTIPPSLSTTTYVTKAPPPPSTTSPTAATTTTISTTAPTTTTTTTATTTPLKSNITMSTSKGTTTTTTTTTGPPGSKPAPSMLSKSSATIPFSKPGAPIPSGGKVIARVPCTGKCCEHLEVKPLVNIIKYPDGATTATTAVPGSTAIPPQRTTISNPGSRAPPSNSTPMKSMTTSSPNATPIKTMTSSSSPNTTTTTTTTTSSTSPVNSNYPPPSVSKPKPSTYKKYAPQTSTTTTSTTTTSSPLQQPQQQPMQQQNTYTTTALQQQQPTTSLKSQTIMKPIIKEQPIQQQPILQQQPIQQQQIQQPIQPIQQQQQQTTVISTPSTPSTPSTTTPSTPNTTSTTASVVDLIPAIPIPSLNEQQTQPQIEEQIQTQLMNQQFQLQLQQQRQQQLQQPYQPTKFYSSSNQQVAPSEFSAYVMKEDADRFIETQNRIFHRIPTSPMIMLKNRGAEIPHSLEYILDIPIMHDEPSSKNNPTWGRLILDALRRLVANKKTTRITERLKLDNMYKDMDSRSNQDKKDWKNGLVNEAQYNYLLSLLSNTIKFPDTPDKKKLSASDSQIYPNALNAYNQYQGSLAQSTSAIGTSIHSTNVNNSIVGNGYLSALANGTALNANITSAPISKPSAAEQEFNSMDIPIEINFDSFSSANFDNNEYNMYSNDSYATPNTNTSPETEMLDPTQMMLDNANGLVVGNHSLLNDKDVNSNNNSDIPLHHGGDGNDIKNDSHIGLGDNSSSSSSGGGKDDKHIDIDIDIDSQQQQQQSLKESKMVEVNSSTLLSNDVVKQEKADDDSNDKDSDDSDSDNDKDNSSDKDKQKLLDKSNVDINNNNNNNNNNNNNVDIECNNNNNNNNQNKNKNDLSNTLDIKNVNNNNNNNTNINSNDKFASPDNKNRNNNNNNNNQNNNNNNNNNVNRNNTLNQGFDSYGIIMDIINAPSSTSSLKRKRDDENPPTLPINSAPVSSSDFFNEFNSENYHSQLPFQTPFSPDIVVDDFQIKKRITS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMJ1</accession>
    <name>A0A8J4PMJ1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008813</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000582">
        <property type="protein sequence ID" value="KAF2069873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMJ1"/>
    <dbReference type="OrthoDB" id="361532at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="79" mass="8705" checksum="D26DAA36B0E949C0" modified="2022-05-25" version="1">MGTADHFMPSAIPTLEKNIMIICGILDFFLFGIGTIILGALDNCNVWCVLIGVLQLCLPFVGWLWSIIWGVIILLKGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMS7</accession>
    <name>A0A8J4PMS7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008837</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000586">
        <property type="protein sequence ID" value="KAF2069847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMS7"/>
    <dbReference type="OrthoDB" id="10626821at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="208" mass="22291" checksum="2237D5D0CD543C35" modified="2022-05-25" version="1">MHNQQTSPHCIYDLGKLLNDHVTFTNSLDQGGFDARTTGILVGNNGLPFDIMASFYNMDIYNPLQDYQFVEGRDWIDWTTKIPYKAVMEKKDPFKFPVFPHGVPYQNGMELEGVEFDKSCKREDASSQNTKLRKQGSGKVGLGTRIGAAFGAAAAGAGAGIAFVATQIGQGVEAGAVSAATAIGDGVVEGAKAWKDGADIIIQSLPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN85</accession>
    <name>A0A8J4PN85_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008610</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000547">
        <property type="protein sequence ID" value="KAF2070072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN85"/>
    <dbReference type="OrthoDB" id="20747at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032045">
        <property type="term" value="C:guanyl-nucleotide exchange factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.980.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31334:SF1">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE PROTEIN SMCR8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31334">
        <property type="entry name" value="SMITH-MAGENIS SYNDROME REGION GENE 8 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="62"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="271"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="336"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="232"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="274"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="352"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="366"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="427" mass="47817" checksum="0F1F4962761C59EE" modified="2022-05-25" version="1">MEVLSQNNYIFLPGNPTFQNLYKQEEDENSNVVFHSSPISHSKSMTPSSSISSPSSLLLSTPLTSSPSTSSTSLLSSGGLGPQSKSLPNSCSNTPVMAPVSWKKCCSLPRCLLCTNGTPRCLCMNHPSWTLILRVVFFSLSQLYPDKEFFNLKKEVYVYLNDHWDILSPNQNQSHNWRRQVQDALSHSRFFRSGRHTLRCNGYWQLKLLCNPWSYINSKQFETLKVDEQPSSLSSSSCSTSSSPSSSPINLPKEDHLSHISSPIEIAWRISSPNNNNNNSKKLNNINNSNNNNNNKNNNDSFNNINNTLNNKLMDIKQLLHNGTLHNNNNNLNNNINNLNINNNQQDEEYNDLSSPSSNYQSDDAPSSPFTPQSSSKSLHFSALNALSNFSLSQITLPPPPKMVQPSSTDQADDKIYQMKSILNCTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNB2</accession>
    <name>A0A8J4PNB2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007601</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000415">
        <property type="protein sequence ID" value="KAF2071080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNB2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="57"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="70" mass="6949" checksum="95DA5BCCBBF87A03" modified="2022-05-25" version="1">MTLLNHLSAIANPSKAISSSSVSMNGGNSFTSAMSDDMLANGKGGKGGRGPKGGKGNVVVDIDNNVNVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNC9</accession>
    <name>A0A8J4PNC9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Beta-hexosaminidase</fullName>
            <ecNumber evidence="9">3.2.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_008443</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.</text>
            <dbReference type="EC" id="3.2.1.52"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the glycosyl hydrolase 20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.52" evidence="9"/>
    <dbReference type="EMBL" id="AJWJ01000521">
        <property type="protein sequence ID" value="KAF2070235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNC9"/>
    <dbReference type="OrthoDB" id="428480at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004563">
        <property type="term" value="F:beta-N-acetylhexosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030203">
        <property type="term" value="P:glycosaminoglycan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06562">
        <property type="entry name" value="GH20_HexA_HexB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000063">
        <property type="entry name" value="Beta-hexosaminidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.379.10">
        <property type="entry name" value="Chitobiase/beta-hexosaminidase domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025705">
        <property type="entry name" value="Beta_hexosaminidase_sua/sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015883">
        <property type="entry name" value="Glyco_hydro_20_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029018">
        <property type="entry name" value="Hex-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029019">
        <property type="entry name" value="HEX_eukaryotic_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22600">
        <property type="entry name" value="BETA-HEXOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22600:SF54">
        <property type="entry name" value="BETA-HEXOSAMINIDASE SUBUNIT A1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00728">
        <property type="entry name" value="Glyco_hydro_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14845">
        <property type="entry name" value="Glycohydro_20b2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001093">
        <property type="entry name" value="B-hxosamndse_ab_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00738">
        <property type="entry name" value="GLHYDRLASE20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55545">
        <property type="entry name" value="beta-N-acetylhexosaminidase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0458">Lysosome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035188797" description="Beta-hexosaminidase" evidence="11">
        <location>
            <begin position="18"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-hexosaminidase eukaryotic type N-terminal" evidence="13">
        <location>
            <begin position="54"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 20 catalytic" evidence="12">
        <location>
            <begin position="153"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR001093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001093-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF14845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2070235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="531" mass="59330" checksum="6C2586A24688028C" modified="2022-05-25" version="1">MKYLLLALCVAVVAVVAQQPLSVVPYPQTVQMGSGSVTVCPSKFSVTTNSQSTVFAVALKRYQSLFFPFGNGSQSGCVGVTVSITVSSDDENLFLGVDESYTLLTNGQTNTITSNTIYGAMRAIETLKQIIQYNLNSQTYGLVQVKIYDEPRFPWRGFMVDTARHFLPKNAIFHIIDALGYNKFNTLHLHLVDAQSFPVESNTYPNLTNAAFGPGATFSHQDLQEIVAYAQTYGIRVVPEFDIPGHSASWGVGYPDLVANCPAYAANINNIALDVSNPNTYTFLQNFFNEMATIFPDQYFHTGGDEVVTGCWAQDPNMQSWMNKMHFSTIDALTYFEHQLSNMVTAMNKTKMIWNDPFDYGCTLSTDQLVQVWLTGSLPNVLNAGFKAIVSFAWYFDKQIPDGNTHYEWQDTWQDFYNADPYNGITNNSENILGGEGTMFAEQISHVNWDVRVWPRAIGLAERLWSEQSVNSVPLALPRIGAFSCDMSRRGILSGPLFTDYCPLPEDLTLSPLKPVMEIPKDIMQKLINRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ93</accession>
    <name>A0A8J4PQ93_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007799</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000436">
        <property type="protein sequence ID" value="KAF2070881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ93"/>
    <dbReference type="OrthoDB" id="3784at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00761">
        <property type="entry name" value="Glyco_tranf_GTA_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050834">
        <property type="entry name" value="Glycosyltransf_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43685">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43685:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 2-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="1">
        <location>
            <begin position="58"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="429" mass="50166" checksum="3AC53DB43F88C115" modified="2022-05-25" version="1">MLSSGSADRSTSYHLDHYTTIVNNSNKDEIPENEYLHTNKYHPTILSNVSPNDSFQFSIVIPHYNQLSFIQSTMDSVFNQKCDNVSFQVTIVDDGSTEWNTKEKLLSLFKHYEQYLNITVIFNRNSGLSFSRNIGIENSRAEWILPLDSDDMIDSTFLLKSWTLIKEQDELQNNIIIIPGLRYIDYKSTFAHLTQNVKSIELGEWEIPVWNANDIKYRNLLHCSGIYRKSLWKSIGGYNPTLWFGWEDWDFWLRADRATKGIKPLLIKGEPLFNYRLKNGMHSFCKENYQLCFSLFQTIHHTEYSIEDVIMAQKYIGIHGQSIKSSIMSKIKVFSDNSILHFWMALIYQYAQYNSETQKFSNISKANDYYTMALDYSPNGVTWQIILQKSLMNLGSVNNYLKSKKDLLQLYEKNKQLEELVLRIYDVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQB3</accession>
    <name>A0A8J4PQB3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mitochondrial cardiolipin hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_006915</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phospholipase D family. MitoPLD/Zucchini subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000340">
        <property type="protein sequence ID" value="KAF2071769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQB3"/>
    <dbReference type="OrthoDB" id="5205528at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016891">
        <property type="term" value="F:RNA endonuclease activity producing 5'-phosphomonoesters, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051406">
        <property type="entry name" value="PLD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43856">
        <property type="entry name" value="CARDIOLIPIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43856:SF2">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035240814" description="Mitochondrial cardiolipin hydrolase" evidence="3">
        <location>
            <begin position="19"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="4">
        <location>
            <begin position="154"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="4">
        <location>
            <begin position="346"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2071769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="430" mass="48569" checksum="CF5F29C00C9F9907" modified="2022-05-25" version="1">MMIKVLFVFVLICGVVLSQDASNDYQNTTYNAQFEPVSQSGSISVTAYFSPDNSVGLETELVENAKNTIDIGIPGFDSWSYCTDTYKGIYGCTVDKQRNQEAFPIFQALVNAINRGVQVRILTNYYSNTTYTPGYIDPLAFLKLQGAQVRYFTTLTFLHTKYMNIDGTTSSISSINYSQTSFMKNREAGVIIQNANQLTNFTNAVFEYDWDQSVDWPTISYSAADMKIIQDKSPLTVTIPPPNTFSGAYITKPTTVATKQTVEVFASPDFAFSNVAEDIKKSSSMKVYIYQITGTTWCDIMANYPGKLTILVSNDIFDQADWEAATQCYTQLVQKGITVRKTAYGMYTYSHQKYWILDDKIVYLSTGNMGDTDFPSGSNEFPAYDQDPSNWRKTNRDFNVKMTDSQLVEVYETLFEKDYALGYDYHPKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQJ6</accession>
    <name>A0A8J4PQJ6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007676</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000423">
        <property type="protein sequence ID" value="KAF2071000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQJ6"/>
    <dbReference type="OrthoDB" id="46159at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024395">
        <property type="entry name" value="CLASP_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567">
        <property type="entry name" value="CLASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567:SF9">
        <property type="entry name" value="CLIP-ASSOCIATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12348">
        <property type="entry name" value="CLASP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TOG" evidence="2">
        <location>
            <begin position="30"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="421"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="455"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="673"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="728"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="310"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="347"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="421"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="463"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="483"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="523"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="683"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="728"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="759"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="777" mass="84889" checksum="16B81767D9EBD2C7" modified="2022-05-25" version="1">MKTSKPTLTPASSIQSRVFSPAKQETSVLNFDDEKELQKKIEEIFFEFKNKDTRDWSFRYKSLIVLQRIVNGNAIEFKTWTSLLRYLSPQLIEQLTELRSTIVKEACTVVTLLAQRMGSKFEPFASQYMQALIKMVIVKTAIISDAAHVTIRDVLMCVQTKNLLTMFLNGSLDPKNEQLRKRCCEYILIVLQRAIEEPGMILTTSVPALEKSISHLLVDGGSDVRLTSRYCFWAYSELAQDAAIKMMYTFTPTTQKNLFTVVDSLPAAQQEVAEKIKQSLFEEEQSQRDVGDSNDLDFDINDFKKDDSSNDTTTTSTTSINRSKTPTSSGRMSGLKAPRPSLGGGTTTKSTTTTSATNSTTQIKRAGSSLGMKSTVVAEPERTIKKPVDSASIIRSKSSLGTIRKPVSTTATTAAISSALSSTLTKTTSTSPNLSSTQSGRYSSIGTRAVISSSSITSPTLSKQTSSSNLTRSLPPSSKSPISATLTKSTSPTTSSSNLLTKSASSASSPSTPSSKIPAKRMSTATSSPTTPVSSPSTLTKNKATTTTTTTSTSSPLSSTLTKKTPTSTSTTASPLLSKSKSLPSASLESNKKRLSTEVKDKVTLTATTDDSDTILQQSKRESLNLDSFERSLNQNNNLAFHEISESLAGSIRDAKKGLSLYELESSTNELTLDDLDTDRNKNSSSSNNNIFNHNGSSSANGRFQFSLDNIKRSKSIEEDFKWIEEESGLDDDNDLIENDPFDFNDDDDNNDDQDVILDQEQQQQQQSTIADDDEFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRB5</accession>
    <name>A0A8J4PRB5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 5B</fullName>
            <ecNumber evidence="3">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Translation initiation factor IF-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_006824</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000332">
        <property type="protein sequence ID" value="KAF2071857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRB5"/>
    <dbReference type="OrthoDB" id="4928at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03703">
        <property type="entry name" value="aeIF5B_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000013">
        <property type="entry name" value="eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10050:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000112">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000113">
        <property type="entry name" value="Translation initiation factor IF-2, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="entry name" value="Translation initiation factor IF- 2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029459">
        <property type="entry name" value="EFTU-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003078">
        <property type="entry name" value="PRK04004.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF4">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14578">
        <property type="entry name" value="GTP_EFTU_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="Initiation factor IF2/eIF5b, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="14">
        <location>
            <begin position="430"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="347"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="81"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="127"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="174"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="191"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="235"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="351"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="370"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2071857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1017" mass="113200" checksum="9EA50DC838A3A166" modified="2022-05-25" version="1">MGKQRPRKDNYWDSDQFEKDREDAEKIEKGEQLASSSSESEEEVKPTKGGKKAPAKPAPAKAKKQAAATGGKKKQQLSSESESESESESESEEEVPVKKGAAKNNNNNNNKKPAVNNKKKQVLSSESESESEESESESEEEVPAKPTKGGNKKAPAKPAPAQSKKQAAKKQESSESESESESEEEEEKKTTAQPSKPTAKPAKPTAKGGKKAAAQQNKKTTTEEEPKLSGFLLRQRQEEERIQKEKEERERKAKEDKERREKQAIIDEEKARIKAIEKAKRKSEKREKDAVENKEKERLEKLAKLGVTLPAAATEVGEKKKVVYAAKKKQPKQNTVAQVTEAMEKVAVEDENKEEQEVADNWDDDDWETIDEKEKKKEEEEKKKEEEEERKRKEEKAARKAAKAENKKPVITNAPSIATAGTSVVEEKVYRSPIICILGHVDTGKTSLLDKIRHTNVQGGEARGITQQIGASFIPVENIKEQTKAFAEKVKMEFKLPGLLLIDTPGHESFNNLRSRGSGLCDFAILVIDIMHGLEAQTIESINLLRMRKTPFIVALNKVDRIYDWKPCVNTEFKEAIKLQSRAAAQEFDSKVKETIAALAGQELNAELYWRNKDIKNYVSLVPTSAHTGEGISDLMLVVMQLMQKRMIEKVVFTEQLQCTLLEVKVIEGYGTTIDVILVNGSLNEGDKIVVSGFNGPIETTIRSLLTPPPLRESRIKSQYIQLKSIRAAMGVKIVAPGLEKAVPGTSLYVVNDDDDIEKIKADAKKEVDNVLNDIDTSGVGVSVQASTLGSLEAFLSFLKKIKVPVGNVAIGPVHKKNIMSAAIMKDKDPKYAILLAFDVKIEESAQQTANDMKITIMSEDTIYLFEQRLKDHFGAIKDKLREETAGTCVWPCLLEVVKVFHKADPIVVGVKVKEGSLRIGTPICVPESNNAFVGTVTSIKNNEKEVTIAKKDDTVSIGIDDSAAKTTFGRHYDEKKTWFSRISRESLDSLKEAWGEELTKSDIQLLKYMKTVFKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRN0</accession>
    <name>A0A8J4PRN0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_006602</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000312">
        <property type="protein sequence ID" value="KAF2072082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRN0"/>
    <dbReference type="OrthoDB" id="21582at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
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    <sequence length="987" mass="113536" checksum="18C1C6CC9CCDA5E0" modified="2022-05-25" version="1">MAQLSKLEGTAIIKNPYEDMNYNDCEILPPNLSILIDAAISSETADIKGDYSENNKIDILNISRGEIETHSNGYTTTTNTISYHHHHHHHSLDNDINNNNSYGYINNHHNSTGNNQNSCNNNHHDSIDMNNNNHNINLKNTFKNYEVSKLPLRKRHLVPDTVYYNFYQNTFVSPQSPFNSAPSPPSPKLTSSGSSFDQFDNVDGKQDMLEQRVPSPSHSNNNNNSNGNINNNNNNNNNSNENKKSPDYIRESYFVDNNSHQKLLVEEDDVDVEVVDDEENPLNRTKKLRKLKNSKVLLEPPLGDSIVSDNNNNNNSNSNSVNNSSNNSPLLESKLISSSPIIKEEKVESFNSNNNQNNNTNNNSSENIKPSTLKGSIMEDEKNDVIIDSPTKTDRRDSISANEDIEVDILVEEDETLKSKSLKLKNQEKENNFNDINNNMEISKKYHHREKEHSREHREHSREHREHSREHREHREHRDREHREHRDHKEHREHRDHNRENKEHRELRENKEFCDEIEMDLDKELLERNHRHHRHHHHKKRVHRSSHRRDKEHRIKKFKSSKSDNIDIFESDQSDESDSDEPNVKDIDDKLYCVCQKKYDPKQFMIACDKCDEWYHGACVNISEKDAKKIKSYLCVKCKKKEKVSPISLSQIVSNHHSTPSHSPRSSPTNAERELKKSLSSSPLLSPSSPSFNNNIINNNNNNSSNQLVNNNNNTVKQQPQGEIRKCAYSKCNEMANGTSKYCSRDCGYANAKDKVFKNHQHLLHKSSVSVQSVSITSSPNTPPSSSSNNSNNNNNNNNNVTLSSPLTSSTNNSLLNTSSPPQNSTDPTQKLLSSSNSEDFNRLKFISQKRQEVEEKLKSIEKKSSEFEKAILLSKLKLESSIESCNPKVYNSNNNETDNNNSNNNNNINEQLCIYPIKESTGGFCGEFQCSKHIGWEKKRRETFSKEQSFHTKTLRELSLEEKQIHQRISRRNKIAQQNTDPILDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRN8</accession>
    <name>A0A8J4PRN8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein transport protein sec16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_006236</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the SEC16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000279">
        <property type="protein sequence ID" value="KAF2072443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Sec16 central conserved" evidence="10">
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        </location>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2072443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1724" mass="192333" checksum="67502EB9B47F220D" modified="2022-05-25" version="1">MENQNFFGDDGSSFFDSLASQSDSNSNVESNVSDITAAEDIILDKTISEKPQTLLPEEHQQQHVEHSPSSQAVVANDVIAKEQEQVQQQDDQITQGEQQEQQQEQPPIEINQDAVIVDNSPTSDTVVVNQQEQEQIDSHNNNNNNDNSNNNNQVPENITDNSTTISDTNSIGSFETNNSGVPAASEKDISSFFNTSSSDSDFFNQQVEQPQQQQEQQDSVTSASDIQQQQEKGKEQELQQQQQQQQQQQQSINTITTDVKVDQGNDFFNNISTSGESNQQEQQQEQPIDDHLSNDAVSSTSAVASSTVAEEKDVSSLFSNSSVTEEKDVSSFFSNSSNQSSFFESSSIDTNNNNNNNSNSKQQQQQPQKDLSSMFSSSEREVSSLFGNMNLDENAMFFENLGRQQEIEAQQKLEQERLERERIEQEKIRQEQLLIQQQQERERQQQYQQQQQQQGYNAYNNGYNNYGGYNNNQQQQGYNAYNNNNNYGGYNNQQPYQQQQQPIMNPYQQQPPPQPYQQQQQPVMNPYQQPQPQPQYNNNYSSSPSRPPPMPIPIPSPTRDQQQVNGWGMPQQQQTNSPSSWNQQQPPPQQQQQVTPPPQQASAPIPMIKKPPPFSNHQRQDSSDQFTKMLNGQVSHGSPSQQYPPLQPQYGQQMNQTSPVNQQQFSPNQRPPLPVQQMGVSPPPPVVPQQQMGVSPPPPIAQQQVSQPPVVPQQQQIPPPVVPQQQVPLQAPLQISPPAPLQQQQQPPQSHSVTQPMPLSQPMPIAQQQIPLISSGGSVPFYNNSPSSSFSNSGMSQQPPQPIQQITSPPPPQQPPQMPSYQQPPPMFNNNNPMNIPIRNNSVPDLTHHYSSSPNQYPGSPSHAQMMMNQQQQYGYPPMGQSPSQLNPMMQQQQFHHARSTSWNGSDAHQRGLPFYPRPHSVVAFGFGGKVVVIKPKTDGQQQVPMTPNNNNGAVQSIPASKNTVSILLLKNLLSKSTFYNNASTFPGPLSSSSSKDSLLKWIGERTHDIASGNIDTFCSHDPEASHMLWELLKILCNNYGQVLNSNNNTGEGEDKNINKQIQALLLDNKSHMTSFSQVIDQQQQPHHTPTDTEMNVLLNELQKHLIQGNLQAAHQLALHNRLWPHCLILAHQFGPEVYFKTLSAFNATLPMGSPIRTLYSLYSNNTRELFSTEFKDQWKENIATLLFNGKANGCKVIGEIGDSIWANQKRVEAAHVCYLLADYQFGPLNPNSRIVLLGGDHKISKNYITSESLQRSEIYEYSKILGNSQYSLPIIFVYKFIYATYLCDLGLIDQSLKYLNHLKQLLKNHPTQNACFVYQLDTFAERLLSFTSNNKLSASGSWIPSIFTKGFKISFGGNKQPSSNNLTPVTTPTKADNKTPPSAVQQQPLVINNNNNNNSNSNNKKDDDDDDFYNSFGNKPKAPTPAFDPKLDEKETDANAIGTATTNASTPVGKGGKDEEEKEEKSGGWFFWKKNKPKQMILDENTNFYFNEQLGTWVEKGKEHEAKSQMAPPPPPPNDAVMMSSSPSIPPPGGMGMNSPPVNSTTPPPMMGGASSPLPSRAPTGFNSSSPTGGSDDGSGISLSNSGVNKYSLVSPGGPNTRRKPRYVDHLNQGAVHNGGSPMPPRVEQQQPSAPAPFIPFVPKFDPNDPQNVVEQPQPPMQPTPFQPQQQFTTDQQPPQMQPMQPIPFQPQQFTDQQQNVDQSTPFQPPPQQYPPQQYPPQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRX6</accession>
    <name>A0A8J4PRX6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide-like helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_006061</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000263">
        <property type="protein sequence ID" value="KAF2072623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="421075at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034044">
        <property type="term" value="C:exomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055087">
        <property type="term" value="C:Ski complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015374">
        <property type="entry name" value="ChAPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039226">
        <property type="entry name" value="Ski3/TTC37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15704">
        <property type="entry name" value="SUPERKILLER 3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15704:SF7">
        <property type="entry name" value="SUPERKILLER COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09295">
        <property type="entry name" value="ChAPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13174">
        <property type="entry name" value="TPR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="530"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2072623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1694" mass="192480" checksum="33C4FC4ED2720782" modified="2022-05-25" version="1">MDYIKQCLKDAKEAIEKKDFTKALEICTDVLRYDDKNFNVFLFIGLSNYNLDRYPASEKGYLSASKLNPSSPFPLRGLIDLYTKTSEYEKLVMTINDIIPLISDASKKKEMYIKLLQYYSNPNLVLTNVPNRYSDGINIINKFVLPEIKQEEVDQLWKLYDQLENFTYLDYSQRFKTATEEIMKSKNIPIINPAKAHVIGAPTHTPEQKIEKEKIDNQVDSELLQSLNLELYKDILFNHTGVQEYVDKNIHTNESIRIVLERMYSNYIQFLFKLRNCCSNTEEVGQEIYRCCIDMKSRCDNPCFALETLICLYEENGSLVDDKQLTEVSQVLHNKHNNKGLGWIGVGYCAVYLNKEYGNESKAMLEKGLNSTPESILGFKGLAKWYQESNNGGALDRVVTAVAKGLQVAQTKTSRMGDIYSDISLSLQMILVETYTSSCENEKATKLLLSLLEKSPNNPQILFELAKLYFDTKNYQQAKQHLSKLENIDDQAYKQLAHIYLGWIAVVDSDGDINSALSMIQTTMSSQETALGLYVLGLVYKKMDKKTEATQSFLKSAKLNPNYSSTFSQLASLYRGVDNERSKKCYQKAISLDILNQDAGLSLADIYVEAGQISLAVSLYKEITTHCLANRKQFPVNVVLCSWAFYRLALYQMDNSQLEDSANCFLNAIKGDSNNAAYWRGLGEAYRRQTKYIASLKALKKAEEIVCSSSAAPELHFQIASLDYTMGLYQDAVYEFEQVLKSIPHHVPTLKGLAQCQLDLAKDHHKAKSFNQAATCLMAAEKSIKMALDDQKSFDSCWKLLGDISTFYIYLPSTSPDYSFNALEKLVQGSEAYIQCVKIRSNAASLYDLSLNYYYQTLYLLNKDQQEQQDNASKLSIKCITQALNIEPNDARLWNAMGVVLMDRYPLQSQHAFIKSIQLDSSKPQPYNNLALLYLQHGFVDQANSALMIAKSNNPDSVVSWSIQGLIHEIKSLYGVEYLEMAKNDYHHIAIALESTPVGEALLGYGISCFMNQQYDIAYSILYKFIELYPTSIDGHNYLSLVYENQRSYPEAIQHLHRAIQLVSNQKDKKSICKQYTNQTSSSIGSLFVSNQTTANLTPSLGESNLIELSYQDKLKLLSINLSRCQYLNGQYKESLETIKPFGDVSTANVFGNSLLLEITGLNHYHLGNIDQAIDFIKKSISIMDNLSKQEEMNTDRKLDLSLLLAKLMYRKKAADIKSIDALIIPILLSKNKNNQEKLWFFLASIYIIAKDYNKAIDVLGKAEESGVLSSQLFFLKSSVYIIQGKQDVARRHLLKSVHMYPHDPLTWTKLAQTEMNFPSDPSPLPNSLMGHASIAKENSNDIINDNSIYETLARSQLSRVASPYLVSLNQSISSLKRIVHTNPTLYMPWYYLQNALYYKSIYSNDIQDYQATQSCYNIVMSKHLTDATKYPTTVQAFEFSQSLIHLKLSATLDQPNFIALFDKLLVQHKDCLENKVELLLIKAKHYQDVSQYKQAIQVYKEALACSQSTLSIYHNLSFIYEKMNNFEASLLCLNQCLSLITHNDQETGDDSQIGIYSTTIAKIARIHILQRKFRDASKLVDSALSKQSKQPNSTLLLIKAISILHQSKPTSNNVKQFTNDMVQCNQLLSDSISSNSKLTLAHYYKSITLLNLKQYKSVSQLLQEELLISPHLEKDTQNVFEKLKVSLSQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRY2</accession>
    <name>A0A8J4PRY2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006641</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000315">
        <property type="protein sequence ID" value="KAF2072051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRY2"/>
    <dbReference type="OrthoDB" id="442460at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10537">
        <property type="entry name" value="SET_SETD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040415">
        <property type="entry name" value="SETD9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33524">
        <property type="entry name" value="C5ORF35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33524:SF1">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2072051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="444" mass="51346" checksum="FED032540A4A9153" modified="2022-05-25" version="1">MIKKIFNSAVSKFGSKAKPSTTNKYFQNDQEYLDTLKVYLTTEVYRELYYNHQGDYNSFLKAEEFIDSLHDGAHKIPYLKQSNRIEEIINHQVLEIFESIHSYGGKNSYPPPLKESTFLYRSMEEKVKRCLLIKETHNHGEPIEHPRGFKLHIKPSMLDHKEAGYGLHVEGDIFPGTVIGFYPGHTYDGNILPAQVIMDNDYMIARYDGTVIDGRGWTRKAEELMLKNELHQVLGSSGGRHTDLLRFKNPFAIGNFINHPPAGVRPNVIAYNFNFKRNFPQHLKPYIPNHIATEDKTFKDDSLYLRSLVLVALAPIQDTELFLNYRFNPANKYPDWYNDPDIEESKRRWGERERLSLRMWEYSIEGKVDVGGIPALLFFSELPPSSIAAIPFIYIASINSNGFIDDINSFHNEYINTSMVKNSDRNNNNNRGRSEMALLDRYSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS21</accession>
    <name>A0A8J4PS21_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005245</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000204">
        <property type="protein sequence ID" value="KAF2073428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS21"/>
    <dbReference type="OrthoDB" id="29321at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="599" mass="68549" checksum="1512820599A64135" modified="2022-05-25" version="1">MQPQLFFSIFRNRYLKQKIFISSYDYYIIKNISHLEKQYDHLCLLNSNNIPVVYYIPNIDHYHIYCSHKHKSLITHIIVSSHFYEYHYHNLLQESQPLPILFGLTITDRERDPIILANKLPPSVQYYKYRIGGSAYDGYPEPIQKGLIPESVTYLHLGLDRLDADVLPNKVKTLVLTLQSIEDLPKKCFTLQSIEYSPKKCEFNGRYIPRSVNSFTLYDYHHKFNDLDINLLPTNLTYLNVNGNIKNHGFGSLKYLSMTCSDICPKINIPNSVTHLKLLNRQMDKKSIHLSPNLTFLHLGRTYQEYKKTIPIPEPLPRNLKTLRLEQYSNKAVTKSNCPANLLSYSNVNTNEDKYLPYIPPRATKVSICFSHRPKTKQQIPQIVPLTVTDLKMNFPYHDIPKRYFGNNIVKLDLTSIGDLTPDTIPPSVTALSMNLNPTTIEKLNSGLLSEIIPNGVIKLVLICNHSVVPKSMKFPDTVTSLSISSYIPNAITLVPTSGNLKTLKVSNLDFKTFSLTSLPPTLSKLCTSTLGSNAFQLTYGIVCPNYDNSKFPSNIKQVKFKNIHQYGFDHSFHFIDGGKVYAETHKPLNPFLSRINKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSU0</accession>
    <name>A0A8J4PSU0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006088</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000266">
        <property type="protein sequence ID" value="KAF2072591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSU0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2072591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="68" mass="7537" checksum="E5D1676C80FBF7DF" modified="2022-05-25" version="1">MLLTNLSQIVQPCKKISSSLSLTQSYISLTSSNSLACCYVPEPKVESKPESPKELYCKYCGCELIETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSV7</accession>
    <name>A0A8J4PSV7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_004762</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000174">
        <property type="protein sequence ID" value="KAF2073938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSV7"/>
    <dbReference type="OrthoDB" id="370281at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17326">
        <property type="entry name" value="MFS_MFSD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051068">
        <property type="entry name" value="MFS_Domain-Containing_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23510">
        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YAJR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23510:SF3">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="85"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="315"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="346"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="375"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="412"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="443"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="46"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="472"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2073938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="499" mass="55474" checksum="C5A5078D94C979D3" modified="2022-05-25" version="1">MSTKSYHQVDDDENEKVIVVTTADNSNNIDSSNSEQDEAKKRVAKSINIMVLTVFLQSIGFTVMMPSMYDYLEWIRPSFGDYNGWVVAVYSAGQFLSSPFFGYWSNKRDAREPLIVSIIISIVGNILYAIVYKFNGKAVIIMAIARFIVGIGAGNVSVCRAFASEVSDISSKTTTMAKMGAAQGAGFVLGPAIGYALAYCNFHIKDLVINEYTAPGYLSVLFAFANITAILIAFKDTRDIKSRLEKEKIDIETAKANSNSTGVKRPWYYQKNQKLLPIFVSIFLFAIVISIFAVFETILTELTKRYYNWEVKQNGYILGGTGILSIVVFVVISLPFIKKFDDRKMVIFGFANLTVALLFLANYGKPFNWETDLPLWQFIIGSVFVSIGYPIASSLIYAIFSKVLNPTSQGTKMGWLTAGGSLARMLGPIWAQSIWNKCNQEGMVLFLVTASISVVAIITLIVFFRKLDPHPDYKPPTPPPPKTTSINADEYDESKPLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUA1</accession>
    <name>A0A8J4PUA1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005409</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000215">
        <property type="protein sequence ID" value="KAF2073280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUA1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="241" mass="29827" checksum="3B257358C51968AE" modified="2022-05-25" version="1">MIIDRYRAVFTNIYLATKIYNTVHQIQIDRFPLKYHDIIDVGWMLKYGHVGLVREKIKRNEYLYNKYYALEYYDKGFDVVFEKLVNVDVVKYLFENGYARDLTILDYINKNNWQCFTKKALNKNYVHAWYIEIPFRMRIWESRRPSPINIPLVEYLIKEKYISFEKVKKIYRYLEPFFGGNDVNCILQEEFWFHEFVYLIYHNNYFSIDPFMNLLTNDSFDCWLNYFEYIYRNQHKFQTKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUL1</accession>
    <name>A0A8J4PUL1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">glutaryl-CoA dehydrogenase (ETF)</fullName>
            <ecNumber evidence="11">1.3.8.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_008819</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>glutaryl-CoA + oxidized [electron-transfer flavoprotein] + 2 H(+) = (2E)-butenoyl-CoA + reduced [electron-transfer flavoprotein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:13389"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57332"/>
            <dbReference type="ChEBI" id="CHEBI:57378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.3.8.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid metabolism; lysine degradation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Amino-acid metabolism; tryptophan metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.6" evidence="11"/>
    <dbReference type="EMBL" id="AJWJ01000583">
        <property type="protein sequence ID" value="KAF2069866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUL1"/>
    <dbReference type="OrthoDB" id="435240at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000062">
        <property type="term" value="F:fatty-acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004361">
        <property type="term" value="F:glutaryl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046949">
        <property type="term" value="P:fatty-acyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01151">
        <property type="entry name" value="GCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000003">
        <property type="entry name" value="glutaryl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000006">
        <property type="entry name" value="Glutaryl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000008">
        <property type="entry name" value="Glutaryl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052033">
        <property type="entry name" value="Glutaryl-CoA_DH_mitochondrial"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42807">
        <property type="entry name" value="GLUTARYL-COA DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42807:SF1">
        <property type="entry name" value="GLUTARYL-COA DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0274">FAD</keyword>
    <keyword evidence="4 13" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="16">
        <location>
            <begin position="46"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="15">
        <location>
            <begin position="159"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="14">
        <location>
            <begin position="265"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2069866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="416" mass="45617" checksum="AA49F2BBEA8C8EDD" modified="2022-05-25" version="1">MISSISKSFVTKVLKANTINYGVRSFASGVDKFSWEDPLCLETLLTDEEKMIREQARKFSQEKLFPRVQMAYRNENFDREIMREYGSIGMLGATIPEYGGVSHVSYGLMANEVEKVDSGYRSAMSVQSSLVMHPIYTFGSQAQKEKYLDGLASGDLVGCFGLTEPNAGSDPAGMQTRAKKQANGNYIINGTKTWITNSPIADVFVVWAKDDNGDIRGFVLEKGMKGLTAPKLEGKLSLRASITGMIVMEDVEVPATNMFPEVKGLKGPFSCLNKARYGIGWGAFGAAEFCYATARQYGMDRKQFGKPLAANQLYQKKLADMATEISMGLLACHQVGRLIDSGKATPERISFIKRNSCGKSLEIARNARDMLGGNGIADEYHIIRHAANLETVNTYEGTHDIHALIIGRAITGLAAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUR0</accession>
    <name>A0A8J4PUR0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004754</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000174">
        <property type="protein sequence ID" value="KAF2073930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUR0"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004767">
        <property type="term" value="F:sphingomyelin phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.270.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038772">
        <property type="entry name" value="Sph/SMPD2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320:SF19">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320">
        <property type="entry name" value="SPHINGOMYELINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="388"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="443"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="398"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="451"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1042" mass="118103" checksum="CA82E897E038BB9E" modified="2022-05-25" version="1">MNFNSHPSDWKRELKKASRSCLLNITNETDFQLQRISCKMQQGFLRFIPPEIIPPKSSIEFGSESNAFMSGTKGNVMYVVLPPSVAPLTNVGSIQSITHIAQMKLWEPFIYILIEWSTPYWSSYSCSIQCHPANNNSLSTRHTLQMKDINKTIHTEASMFISGDLNFKVYKNNISPSLSPISSPKLMNNSNGGLNSSGNSMSSSIGILDNGSNGNNNGGGDRNSLSSSLPIGFLNGINNSGGGHSMLFPLPWSTHNEDSKDIGWKALVKRGSRGQIISIRNNTNKHLIRRNQILNNGTWCEPPPEGIPPFSTIEFGSVSSGFTTGTDGTIYYYCQGSKGDFGFQFNNPLMGKATFSSHCPSNFQIEDKFTDGNISSVIFSITDLSSSLEKDKDKDGKQQNNQHQQQQQQSNPQTSFILDDSIRVFSFNLGMLGSKDTEQLINGSSKEMERSGSGNNLASLNNSSNSNNSNNNNNNNNNNNELQGIVNFEKRVELIAKSLINFSEKYDIIVLQEVFTDTSKDILVKNLKIFYPYIIDKIGENSGLFFASRFPVLWSEFRQFNNGIGSDVKHSKGIQGVKLDISTVREKSYLYVFNANLQSNPSNNSVAWQMVQDDKQKKAETVRTLQLQSIRDFISTELAIQSSSLSSSALLLLGDFNIQAEMEQIISDEGSITLSQIIPELAKKLNNKELLITFSYRLLEHLEDLNIPIRYLGILRSQLLNDRLKSLVLTEMITFVIKKDIIDHLTQRHNQHSSINDDEQIYREIVLNTLNNVFHYERKESNFFWKVHLKKRLKAEFSSSLSDLEQQEWVDLRCHVINLQLYSSLKYSLEITLNPKAVLQIMKGSRINLSESIPLPLIRDDDIEEIILPPLTSYWGTDNSDITYFSNIDYIKNNINNNRHQQQQQQQQQQQQQQLQNENIMDNNNNNNIQIYLKQSQEYQNMIKILGNPTDVFRACNPFSPGYTIHQAMNQMIPKVGAKERKDYIFNFNLSPNFGDQEGRNQLLRLDFSECNVLPMGPTPQTRLSNHFALECILKIKEFNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUV9</accession>
    <name>A0A8J4PUV9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Purple acid phosphatase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008628</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000551">
        <property type="protein sequence ID" value="KAF2070046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUV9"/>
    <dbReference type="OrthoDB" id="45007at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039331">
        <property type="entry name" value="PAPs-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015914">
        <property type="entry name" value="PAPs_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22953">
        <property type="entry name" value="ACID PHOSPHATASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22953:SF119">
        <property type="entry name" value="PURPLE ACID PHOSPHATASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16656">
        <property type="entry name" value="Pur_ac_phosph_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Purple acid phosphatase N-terminal" evidence="3">
        <location>
            <begin position="179"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="586" mass="67193" checksum="73DE2ACB235FCB25" modified="2022-05-25" version="1">MAAFKTYFYLVAEAVIIVLLLLTSLGVGIFGYNKFRKLKHPKNDSALHGLHYAFLTFWMGSPLMIWISTGFNGIPWIAAWILMALIWMAGFLTLFKKQPEYKNDKFKNHAVHYLLSLKEGASKMGPTVVFIVFLVFAGFAFFITFFSYNFCVGLHPFRVTSEISRRMHGHSCPRGQVCNFILTVPEDLSTSIIVNFHTNDQPDQLDSQFPTSFAFYDTVSRRTDQSDPIVPIKDFVNQYKYMSVATNFLMPNIKEEQRYVHWADVSGLSPDTTYFITAGYQLNNTLVFYQERKFRTAPADGTPFRFMVGGDMGATKKGLRLSETAAEQEPLFVMVGGDVAYDSGFACCYRIWDEWFERWDKIFITPNNYTIPIITTIGNHESTGWRTTKDDVQFYTRYLPFQLGLQNVDPQDRPQYHKHLIGNDTVIIALDSDVVESIESQIPFLEATLNSTAQRRFKLGLYHIALYPCTKISLDDDITASAREHWSGIFDRYNLTVGFENHYHLYKRTKQLRGGQQSETGTLYIGDGSWGVDTVLKVYDYDYLAKAGKIQHVMKGTISPFSSKISIESYNSDKIVFDSWNRTLLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUW6</accession>
    <name>A0A8J4PUW6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein phosphatase 1 regulatory subunit 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003324</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000103">
        <property type="protein sequence ID" value="KAF2075348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUW6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004865">
        <property type="term" value="F:protein serine/threonine phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011107">
        <property type="entry name" value="PPI_Ypi1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20835:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PPP1R11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20835">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PPP1R11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07491">
        <property type="entry name" value="PPI_Ypi1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="15779" checksum="C58469DF86C2CFA4" modified="2022-05-25" version="1">MHNTTTSNNTTTTTTTTTTTTTSPRSSSQESSPPTVRLTFKPKKQKKRVQWTENIVDNEHMEKRKSKKCCIFHKKKPFDESSDESGEEEILRKKRKEESENHSCECEHDGHEHHENENHHDDNDKENGNSNNNNVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUY3</accession>
    <name>A0A8J4PUY3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6748 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008586</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000544">
        <property type="protein sequence ID" value="KAF2070091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUY3"/>
    <dbReference type="OrthoDB" id="18804at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040405">
        <property type="entry name" value="DDB_G0275255-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046636">
        <property type="entry name" value="DUF6748"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34411:SF4">
        <property type="entry name" value="DUF6748 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34411">
        <property type="entry name" value="DUF6748 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20533">
        <property type="entry name" value="DUF6748"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035268474" description="DUF6748 domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6748" evidence="2">
        <location>
            <begin position="58"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="341" mass="38151" checksum="AB1780EA04A36F59" modified="2022-05-25" version="1">MKSFLFLILVISTILLVPTCVLGSDNDSTGQASDKLIANEGDAQSAKLMTSAFGTKIYYSVKADTRNCPAPACGGFFLKRINLDDSDEELYVSEIYVTNQNINISMITNLKYLEGGQDNKIIISGQLKPSRTNGASFECLHLTDILHQMELPESGFASPKFQFKPKKTFYAIKASPFICNKMVTNCPNIVSYKINTDEMSFISSYTEDYSLRVPFIHKEWLVSRIFNEDDNTSSIVKGIILEGKLIISKIYVSIVDPVAPCPEIQANCLSQYGLVPSFYRNTDRCPVFDQCVGRGPCHLGVPSCPNGYHLYSLPSRNKGCPHYWCDPDFLQNPSNHMIFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVK5</accession>
    <name>A0A8J4PVK5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004202</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000144">
        <property type="protein sequence ID" value="KAF2074497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVK5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="487"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="157"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="496"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="565" mass="69460" checksum="735F17DF343A8B05" modified="2022-05-25" version="1">MKQEILQDCFLPEKPIHKLTLLSFHSLWLYLDGNGKMPSEFKILSKKLVKMAKGDLLLSSIKIIDDVVQDFFQPPWVEVTDSVQMYMEAWTTINIRIGNLWIKQMLKVAKNLSASDERLESLIGSNHIQAVPISQSKEASKSPFKAPLVEVEDEDELSLIQKEQQKLQMRRKRLEELQNEQDNQKQLQLKEQQNRKEKQDFFQQQLVLKREQEEREKREKRMEDLLREQQKQEQDLQKRLKNLQQLVQQQEQELKDQEEKLQEQKRQQKLKEEQERLQKQILQRQQQEQFERETRIREEKEQQRLYLEIQRQQKLKQEQEQQRLKEIRIQQEEEMKRKEEQQRLLRIQEEERQLKLRIQKQKQEEEERQRIQRLQRQQEEEEKERIRRQRQQEEEDRLYQIRKQRQEEEEEERLYQIRKQRQEEENRERQRLQRQQEEEELQRYRRQMIQQNYYKQDQYEDNFRQQEKQCNHFYLFQMQPSRYESSYKVEPSPGFSQKKISSSESSNLTISDLKGTLKKGDRVEITSNKHSGRRGTFESWNGTNAYFNFNGEKKAIPLQVSVKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW88</accession>
    <name>A0A8J4PW88_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ras guanine nucleotide exchange factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_003927</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000130">
        <property type="protein sequence ID" value="KAF2074781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW88"/>
    <dbReference type="OrthoDB" id="10254377at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.840.10:FF:000009">
        <property type="entry name" value="rap guanine nucleotide exchange factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF333">
        <property type="entry name" value="RAS GUANINE NUCLEOTIDE EXCHANGE FACTOR A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="4">
        <location>
            <begin position="189"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="3">
        <location>
            <begin position="344"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2074781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="599" mass="70122" checksum="982DFA6A94C6AC01" modified="2022-05-25" version="1">MINHGHTNPIEAIYSNQKVDRSQWRKKVLDGNPQINNLCERIYPMDRSIQFALHFHPGKQEIKQKLDKTTITQLILQHLSTMGLKQTRQTLEKESKVSFPNTEALLNESRLVTYINNALRDTERIYDLSMEHTEYNNEEKQAKFAEREEILCQMGLLEEEHQDDGVNIWEEPSENIFVDNIRNEEDDIDEEVVKSGSLNKLVEHLTHDSKVDLQFLKTFLMTYQSFCTPEILMSKLIQRYNVPSSVSESSTRTIQIRVINVLKKWVDEYSSDFNEKLSLQLRGFIDQLSTKYPGPASGILKSLNKIFEESNSINENKQIFNERTPEPNVPKNIFSNRLEIYDIDEEEIARQLTLIEFNLYKKIKPPELLNQSWNKAKLKYRAQNVLKIIDRFNSVSMWVATLIIQTPKVKARARMMSRFMRIAEHLKNLNNYNSLMAIIAGLNFSSVYRLKYTREEVSAQTLRSYQELEKIMNSEGSFKIYRQRLQQGGGGPCLPYLGVHLTDLTFMDENPDMVPVQVSENRTADLINFTKRTLVFKVISLVQQYQQLAYNLQPVHQIQEFLLNIKSDFKAQGLEQYQQDLYRESLRREPKRAQRSDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW97</accession>
    <name>A0A8J4PW97_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4476 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005517</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000222">
        <property type="protein sequence ID" value="KAF2073164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW97"/>
    <dbReference type="OrthoDB" id="13998at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028011">
        <property type="entry name" value="DUF4476"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14771">
        <property type="entry name" value="DUF4476"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4476" evidence="1">
        <location>
            <begin position="5"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="97" mass="11108" checksum="643A45455C1F9262" modified="2022-05-25" version="1">MLDPITDKDLKNFEEQLRGVTQDKDRLRLIKTIANDNWSFASEQVKRLVIAAHYGEAKIQAALLLYPNVTDKQNFKALVLPLFFDDEKASICSKLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWD0</accession>
    <name>A0A8J4PWD0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase M48 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_004366</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000152">
        <property type="protein sequence ID" value="KAF2074345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWD0"/>
    <dbReference type="OrthoDB" id="7464992at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07331">
        <property type="entry name" value="M48C_Oma1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051156">
        <property type="entry name" value="Mito/Outer_Membr_Metalloprot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22726">
        <property type="entry name" value="METALLOENDOPEPTIDASE OMA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22726:SF1">
        <property type="entry name" value="METALLOENDOPEPTIDASE OMA1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M48" evidence="8">
        <location>
            <begin position="343"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="93"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2074345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="566" mass="64126" checksum="08E9DB45F7A8A7F0" modified="2022-05-25" version="1">MFLRSNPSSILNPQRGRIYISLFEKLKHHSCTLNRLQYTSINNNNSNNVRPSEATFICTGGGANGGFCWCKKTSIDTPPRTILNKNHLLNNLGSNSNNTYNNNNNTQDDNYQQQQQHQQQYQYYYNNKDYEFFSREECKARYRMNKHHHCKVRAMAHRLLPIYFSYNANERKNHFGDCLFYYSSSVPNKQNKSNKSKNTTTATPTTATQGSNTIDKVNQHFQNNLANQHNSSFTNGNRSFHSSSSNQFFYSKAARILLPIALTGFRTWIFKGTLLFRIGVICSLLASVTGGYVLLNQEPAPITGRSRVVTFSEAEEKELGKMGYDEIIETYSDFILPDNNNLQNSVKKIAKKLIDATNRQDLDWDCHVINSENVNAFVLPNGKIFVFSGLFQLCDTEDELAAVIGHEIGHAIARHAAERLSVSKVGYLFLTLTRGLVGDTITGNLTTMLSTSLLGLKYSRIQEMEADIIGLEFMTKANYDPNAAIKVQKRFAQMEVENGRSENSIVNLFATHPIGNERIENIEKWVSEYEKSNTSSVSLNHKPQMLVGTKNPLKEEDNYAQSAPNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWM8</accession>
    <name>A0A8J4PWM8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003495</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000110">
        <property type="protein sequence ID" value="KAF2075190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWM8"/>
    <dbReference type="OrthoDB" id="418595at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04584">
        <property type="entry name" value="CBS_pair_AcuB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051462">
        <property type="entry name" value="CBS_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48108">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48108:SF26">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN DDB_G0289609"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="12"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="87"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="148" mass="16315" checksum="E3D882648545724C" modified="2022-05-25" version="1">MNKMNFLVKNLMSKNVMSIKGDATLDIALKSLNTNSIHRLPVVDESGSITGIITDRDLRLAVDSPFLPETNDERIEKLKHHTVSSIMRPNPITIEDSAPIVDAVKLMRVSNIGGLPVVDNKGRLCGIVTRSDLLDLLIRILEPVPPQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX49</accession>
    <name>A0A8J4PX49_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_004374</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TFP11/STIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000153">
        <property type="protein sequence ID" value="KAF2074314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX49"/>
    <dbReference type="OrthoDB" id="4822at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071008">
        <property type="term" value="C:U2-type post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000390">
        <property type="term" value="P:spliceosomal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022783">
        <property type="entry name" value="GCFC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022159">
        <property type="entry name" value="STIP/TFIP11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024933">
        <property type="entry name" value="TFP11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045211">
        <property type="entry name" value="TFP11/STIP/Ntr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23329:SF1">
        <property type="entry name" value="TUFTELIN-INTERACTING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23329">
        <property type="entry name" value="TUFTELIN-INTERACTING PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07842">
        <property type="entry name" value="GCFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12457">
        <property type="entry name" value="TIP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017706">
        <property type="entry name" value="TFIP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="G-patch" evidence="9">
        <location>
            <begin position="124"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="63"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="166"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="675"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="69"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="92"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="107"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="175"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="675"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="692"/>
            <end position="708"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR017706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2074314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="777" mass="89217" checksum="8ED924DDF459B059" modified="2022-05-25" version="1">MNYDDQDIEFNEYIGGEKEPKKKRRYTKDDAIYGVFNEYYMDEDEDLSEEYLKQYKKGPVGFVSSGHYELNEDNQKKPGDDDEKEKEEKKKDKEKKKKKDKSKKSKGNSGASTATTNDASDKKYTGIGLSILAKMGYKSGGLGKEGTGMVEPLKVYVRPKNMALNETIDSKQESDDSGDDSGVDEGGDGVSSTAKRSLGWKKSKPKVKYDLDDHSIEKNEQQQQQPQIILDMRGPEARMYAGALDKLGLQADGSNVISSGPLSELKHNVGLLVNLKKIDINNTDNKLKHERDKVKTLTNGLERLRLTIDNDRQQITKVKSILETVSSCKTELSQGTLTTKTLYKAFKGLLDKYPREFQNFKLYNMDKELLVPLLKKELAQWNIGEKPLQPIKEFRRWKELYDSCTSVLGQELDNYFILIRDLILPPLKLYLRSQWQVKKPKDAVYLISVWYELLPEVISEALLEQSVLPKLKMEIEQWNPRTDPVPIHEWVLPWTNFLKSELETYYPSIRQKLISILVEWQPQDLSAHHILIHWKNTFEGNSMESLINRSIVPKLANAIKEFDINPTRQDTLDPLKWLFVWLDLINPRTILDILDKHFFPKWHMALISWLQQPSADFEEISNWYTGWKKEFPAEISSNDRIKSQFSIALSFMKKAMSGEDIRDITPQKIESIINSSLSSSSAPSQSVKPKSLNTNTTTNTQDKSQGSTKDMLEHWAATNGLLFMPSPRKTDTGQQIYMFSSTPIIIERDVVMHYTGNNKWVPANLQTLLEEAIQQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX76</accession>
    <name>A0A8J4PX76_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005044</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000191">
        <property type="protein sequence ID" value="KAF2073659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX76"/>
    <dbReference type="OrthoDB" id="16457at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="356" mass="41020" checksum="00378A105F679CF8" modified="2022-05-25" version="1">MELLPSLLSGIVGAGLSYALLKNQLPSAPKPRMPQDELNEWNAANDGFCWEKYCELVSPFDNIENFTIIFSTCIELKNIEKHYEALILFIKIKNTIKKLDKSKDSLGCKIQNYIDSDPNLCVIQREAVQLNDLMDAFFNNDGWTNDDVLTSDVQYSYRQEGRRLRSRKFIKVINKPMKNLVMRTAEIDQFSSWKWYQTGKTVDPCEGTNLCSIVSVLSRFVVLNRAAYCWKLNYFLPDGSCVVSYNGANNRQSDYNLPPLPKGFAYPDIFYHAWRFIPVSKNKTIVVSVMESDPKIWFMPTEGFVRTAKKFGCRELALLDQYSNHIRGTVYDEENIQEKSIYQTIDQTVTKISTQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXG4</accession>
    <name>A0A8J4PXG4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PNPLA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_004137</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000141">
        <property type="protein sequence ID" value="KAF2074554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXG4"/>
    <dbReference type="OrthoDB" id="17916at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047499">
        <property type="term" value="F:calcium-independent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019369">
        <property type="term" value="P:arachidonate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07199">
        <property type="entry name" value="Pat17_PNPLA8_PNPLA9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185:SF7">
        <property type="entry name" value="PATATIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="1 2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="3">
        <location>
            <begin position="11"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="2">
        <location>
            <begin position="15"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="2">
        <location>
            <begin position="47"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="2">
        <location>
            <begin position="187"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2074554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="333" mass="37012" checksum="39431BCE12639B5D" modified="2022-05-25" version="1">MIQPKKLKVVVCLDGGGTKGLMTLQMLSHLEKELGFDMTKTCDLIGGTSTGGIISFCKLINLSNEEIVDLYKKIGKEVLKFACTNITESQSIVNTKLYTDSLTKYFGDTHIKDFCAGRKCFLMTSVSEKLVPNYKPFMLSNYDNPGRAHVGPKETLKNLSVVNCVRATSGIPGLFALPTHESQIFMDGGYFHNNPIEATYHEALSVFGASEAEYIIFISFGTGECPNAINSFISNGVMNPLEKLSSFSPFKIDSPAQRIVNFMTKSSNETHLKFKAANPKVKYFRFDPILDKNIQVNDCSDETIAYMNKVTEAYLSSFEVKNSISQLKELLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXL2</accession>
    <name>A0A8J4PXL2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004746</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000173">
        <property type="protein sequence ID" value="KAF2073959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXL2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="76" mass="7941" checksum="DBD424B680BAC331" modified="2022-05-25" version="1">MLFNALSSISNPMKSISNKTVNNGSSASSSIGKTGNQCWYIIGIGGATVWVDDSANPHSYHMGYPPGTFSSSANII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXR7</accession>
    <name>A0A8J4PXR7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ERCC4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003925</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000130">
        <property type="protein sequence ID" value="KAF2074779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXR7"/>
    <dbReference type="OrthoDB" id="5963188at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC4" evidence="2">
        <location>
            <begin position="385"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="112"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="196"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="742" mass="83439" checksum="C7C9A2CDAF924BFC" modified="2022-05-25" version="1">MSIKYNQDTDSDTESISSSDTDKNRDSDMLVEFEVSLKRSDPIKTTDQDFYKKLKLIEQDNLQLMMSFPRDNLPVLPRPFYIREVSLPIPMRLDLMETSSPTQQSPNTPRLSIVPTTQSSSQQIQSQKSITSPPSPPSVGRNKNNSGGNSSNLSTTKQLPEPLGRDRLNSSFEQQFSTPTSSPPTPSSSQTAQEVPLQLSSPTLSPSKAATTTPTKTSTPNKITSSNFVLIPKTPTTPTDPPSKKTPAKPAQSPVKLTPVTGKPVKEYMPQYQSGPFSLLCGMYSIDEPDMTESRIQHAAQQFTGIDTRDEDPNMIFGTLTMLQDLAFKYKLSTMSRQYRKFKITDEGRRYGEICTSFFNIHTQFRELNNIVISTTQPMVTVVVDQCTKSASMERNLKEMGVNCITRKLSGAAGNALFVPTLATPYSDGLDEAHIYPYLIERITLDDFVLCMFEKQVQSMIDILKAFDQIQGRILIVEGSGKNLKKGQAISFDDLKQEMWRLEIEEGIIVIHTISLLESTKIMAYIARILMSEPGVVDNRPLVSFKQLKSAVMADLTSKKIASPATELARNKLYFTFTNDQFKEYILNPDYRKVVADILNSRKDTDKILLIKDINGIKKDIISKGNTNFKKYFLGARDIFTTPDILLVEMLDIEILNFYITQIQISFNISVLLEINTTVTRLLDKNYKLDEEMNALLLDECKEKYKLINAKPIPTQLLLDGPTTTTTTTTTYKDIQIVDLDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXV0</accession>
    <name>A0A8J4PXV0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gelsolin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002865</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000084">
        <property type="protein sequence ID" value="KAF2075831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXV0"/>
    <dbReference type="OrthoDB" id="6375767at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008154">
        <property type="term" value="P:actin polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11290">
        <property type="entry name" value="gelsolin_S1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11289">
        <property type="entry name" value="gelsolin_S2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11292">
        <property type="entry name" value="gelsolin_S3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007122">
        <property type="entry name" value="Villin/Gelsolin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977:SF130">
        <property type="entry name" value="SEVERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977">
        <property type="entry name" value="VILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00597">
        <property type="entry name" value="GELSOLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00262">
        <property type="entry name" value="GEL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gelsolin-like" evidence="1">
        <location>
            <begin position="64"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="1">
        <location>
            <begin position="174"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="1">
        <location>
            <begin position="288"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="367" mass="40771" checksum="FB77E0AE9DFC47FC" modified="2022-05-25" version="1">MNLATETPTLDIAQSNIAQLGSELDKKCRSDKAALEDQWKDCGKTEGVKIWRIENYKPVAWPTSEYGKFYEGDSYVVLRTYSLDGALMNDIHFWLGQKTTIDESATAGFKATELDDYLGGNPTVYREVQGYETENFNNIFPTITILSGGIDSSFKRVEPGKYRNRLLQVKGKKNIRVSEVPLTYRALNSGDVFILDAGLKLISWNGSKSASFERNKGSEVANAIKSDRLGKPEIKVTNEADQDEEFFKLLGGVGPISDAASGGSDLESDRRTSKTLFRLSDSSGKLEFKEVGKNKLNRGVLDTNDVFLLDSGLGILVWVGRKASIAERKKAFTFASEYISKSGLPPFTPVIRFIEGGDNRLFESFFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXW1</accession>
    <name>A0A8J4PXW1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_007205</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000371">
        <property type="protein sequence ID" value="KAF2071471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="3219396at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22117">
        <property type="entry name" value="F-box_FBXL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052301">
        <property type="entry name" value="SCF_F-box/WD-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14381">
        <property type="entry name" value="DACTYLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14381:SF1">
        <property type="entry name" value="F-BOX_WD REPEAT-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="START" evidence="4">
        <location>
            <begin position="120"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="784"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1223"/>
            <end position="1255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="858"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="981"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="8"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="37"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="984"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="999"/>
            <end position="1010"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2071471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1341" mass="153389" checksum="F9D80486E75F4008" modified="2022-05-25" version="1">MKDSKNINTDNNVDQSQSSNHNDSSPSTHTIPILNLSPIATTPSTSTTTTTTSNNKKITPPFNINNQHKNCIIPNSYKDEKLKNNPLVHKKEYTAQQLDELLEFYIGGLKLIGDFEKELNHPEWKFLSRDSGISVWMKPEENSSSNNLLSRNEMVGCLPVQATNYVATWTNRYKWDRYLESSEILYYFTTNDLEKSQQLNVKYSDFDILLIRMTTNNTQFVKSRDCMLFQITGKHPTRKDSYICVLKDFQFSAYPAGGDGYIRAYCSMLGWLFEPIQTSNGIPSCFHTHICNYEFGQDNGGTLTELLLIPMIRKISILNLLYKIKFIRDVLENIDSQMPIESTNTLPPVSAPPSDLFLIGNDIDSRLDWIEYFKNLDSKAEWNSFPSKQSNICIFYDRSSNNPLLIKTKYQLKYNSKDISCFIFEEGFRWDPFIYKVFRSDDYIMLKGGGPIISSQQRYEIPFFSIKSKMYYGATPSIYFVVEHRENGDIIMFTFEQSLFQKNESMFTSYFYVNPSNWAPFTNPSLPESSLAPIRQKSFIDLSMILRSITQSSKKRVQEMEQTSIKVIDLFSDIIDHINHSVFKEESLLDKIKILSIHENSLPPLDDYPIYSNLPPSTRLSPNIQPHTKLTKTEKDESVSSKSNVNVSSPILIDKTTTPNTKMESIRSPLASISQITDQSLNSSVVLPPILDEMDQSPDDVISINKKSLILNNINNNNSNIATTTTTTTTTTKYNTLLYDNSNNNSNNNSPRYSKLHYLLPSFIPEYTDKWYRNQTEQPTMESGANIFYLPHEMVISILSHLDYQSLCKASRTCKYMKEIAESDLLWYRLYLNFFGEIKFNQNLNNTFLDLSLSSNNNNNNNNNNNNNNNNNNSLNTNNNSNNNTPINSPIISGISLNNNNSSNSQQQYDSQLKYIWDSYVKTDFKFQTDEISFNNPSLTYFLSKETRYETITQQWKKNFFLKIRMEVLWKISKFYGPPKNRGPTLTNSSPNNNGMGHQNKTPNNNGNIKNNQISLMTIGSYRISPSLFALIPGEDEGRITSMFWNYERCFLGDSRGDCFMIDFSNLSKAKPIKFPLSPSTSSFSSLLTWNYNQFICSGLSNRVLVKYQENRSSSDCSLLKTEVPIFNSKIPKHWYASGMSSNYHGNRVAIHYNSNSDESEYEDFDTVLDLFDVNTGNLLLSIENENERITSSHSHRDRIIIGGARGSIRLVDCNTGQNVISFLGHQDEIYSAKVLPMEDIVISASKDGTLKFFDERAGPFYIKRIAEDPEFTVKSFDVEPKKILCGGGSRISYWDPRNLSTSIGEIWGQNGLDVIDVQLSQFNVGALYSNGISNIWKFNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PY75</accession>
    <name>A0A8J4PY75_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Autophagy-related protein 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_003586</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Preautophagosomal structure membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATG9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000114">
        <property type="protein sequence ID" value="KAF2075109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2020634at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007241">
        <property type="entry name" value="Autophagy-rel_prot_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13038">
        <property type="entry name" value="APG9 AUTOPHAGY 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13038:SF10">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13857">
        <property type="entry name" value="Ank_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04109">
        <property type="entry name" value="ATG9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="8" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1343"/>
            <end position="1363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1384"/>
            <end position="1401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1540"/>
            <end position="1564"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1625"/>
            <end position="1647"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1663"/>
            <end position="1680"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="13">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="394"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="528"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="560"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1228"/>
            <end position="1248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1837"/>
            <end position="1870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1229"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1848"/>
            <end position="1870"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2075109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1940" mass="220983" checksum="0396A37F8DF7DFED" modified="2022-05-25" version="1">METLPIETLILVLKNLNIKDLVSFSRVSSFYHTIASDDRLWKDLFFNQFIKSKQQSTAAAAAATPTATATTTISVSVAPIASTNDRPISPSTIQQISDTLIKDGIKSNGITPIKGWKHAYRKRIIMELSKKLVEYIQKGDVKMVDYLLSISLLTYNKTYSLASESSIKQKQPIILEKLLKFCSVDNYLDNLFNQSYSRLITLAVSSNSNQCARVLLDHGADITYSNTGVLLMGSKRRAFSLNGFFGSQHILFQMLCKDNIEMSYLFLDSMLAQITFFLCVQSGNELMLQDFLKNEKRGQTLVNKRTGIWDFGNEILKLSDHFSALSTHGGSYGSPRYTIRNQHLSGSGGSGGGGVDQIKIKEKYANMTRVRFTQYKMIEKTLLQFTSSWAPDWCSYTPLHWAAQGGHLGNIKTLLKLGANINTLTSSGKTLYDLAKEHGQTHCLPFLEKQMSRTIKISDFDPLLALIAQGNFFQVTRFINSFLFEKSVKGLDRAIVMRLIVHAIKHNQPRTLSSIFHALSVGHISDIDGNTPIHYAASAGHMECLQITVERGFVNKINKIRQSPLMLAIMNGHSATVQALLQQGASVETKDWLGNTALHYAPPYPDIVQLLLDHHADVSALNLQLRNPIHMASEVLCYESIDAKLESLEKMLAQITHSTMHQPTTQQQERMSQIINQPDIFGNTPLHLSQIHSRSSGSNNSNIITNTLLSFNASKDMVNLSQLSYKDMIVLNNEKQLVQLLKGDGSNTTPLKAPFAMEALIRLNFDVDYDTFVNLKRQNHLMLESYFKRSKSLTEMDKLMHFYSNDEASIQAILDTIKLYQLKSFFFDSLEKIIQGYIQVCRIDLALYILENLGAATNSPLYSQRFLAGVFDMLVHTPHIDLQTCARFVQLYVAVEKNPTIAELNKCLVLDNYNMFTAIVDALLASTEQLQQLFVKDKEEYFTLAVSYGSLDCLEYLLSLDHVYNIDQANEDDYSHNLVLLPKAINGLCFVKKRDQVLDILSRYDVCQIASLLKSLYTPSVDVYEPQQQIHSGASPVLVQSSLEDADIGDTQQDHSNNDTKIQCPDCRLFLLATDADDHKSVCTEGLYPCPNSIHCCSEKPMKKHRLLNHLQNHCLYLHCHQCDSMFSKSDFTQHLFTHEYYTWETCTLCTQMLSAFNTSYKDEIIHIKSSSSSSSNNNSNSKSNPNYSSEYKHLLTCKNHFVKCPSCRISFHITELKQHNCLIIENRSSNSSSNNNNNNSNSNNKEIMFRQSNDGDYEYRQNIQRGGGDYYPLNDNYFMNSTNINNDSGIEMMKDDDSTSLLHESPTSVPAIHNLDSFLTDVYNYFRGKGFLCIFLNSLFEIFSSIFVVAFFSFLVSFIDYDKVFAANSTGSLREVVDFHREVPLWLIVFLGIFSLYWVSKVFSLLVSFKTNWEISNFYHNTLRISEQDLQTIEWREVVSKIVLVPRLCIVKGNMNALDIANRILRKENYIIGLINLKIFNLSLPFPFFSKFKFITKTLEWSISYALFNYVFDQNGIIKKEFLDLNQRNRLSRGLSRRFITVGLLGLFSSPFIFFFLLINFFFEYAEEIKNRPSSLFSREWSPLARWKFREFNELPHYFQNRLNLSYSHANQYVSSFPSEVLSIMAKFISFLVGSFLAVLIVFGIFNDEFLMNFQIFDRSPIWYIGIFGTVVAVTRGLITDENQVFQPAKHMARTVQNTHYLPKGWIGKTHTYQVRDEFLELFEYRIVDFLREISSVLFTPFILMFSLPKSSFAILEFLGNSTVNMEGVGPICEFGAFTNVKKVGDNSYGSLSADNKISQTKQAKLEKSMINFKVFNPEWNTQDNELFKNIETFAKQQQDKKQQQQNPTSFSNPDILSNSNSNNHGSPIFKQNSVDEFHYIQDSHYIPPEIVRAVLGNENCHSGGTINQNVVNAVNDLHQSFYESQIFGKNHNNGHNSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYL9</accession>
    <name>A0A8J4PYL9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GB1/RHD3-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002328</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000063">
        <property type="protein sequence ID" value="KAF2076371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYL9"/>
    <dbReference type="OrthoDB" id="2135133at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01851">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036543">
        <property type="entry name" value="Guanylate-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015894">
        <property type="entry name" value="Guanylate-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10751">
        <property type="entry name" value="GUANYLATE BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02263">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48340">
        <property type="entry name" value="Interferon-induced guanylate-binding protein 1 (GBP1), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="775"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="domain" description="GB1/RHD3-type G" evidence="6">
        <location>
            <begin position="78"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2076371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="799" mass="92438" checksum="495203BC106687BB" modified="2022-05-25" version="1">MIKFFYNNNNILSICFTLYILLVSITTCYTASTTGDKLIENENFKELIQPIQLIYPDKDHQRLLINETSMNILNSLIDYDLSILGVVGTFHSGKSFLLNQLLDTTDRFTVGPTVHPETMGIWVWASKVRYAGKLHNLLLLDTEGFYSSNVSETYDAKIFAITTLLSSHLIYNSVKIIDQSALEYLELLSRRTQLFALKSQMKSESLQSQKKQQSTITDIKATDHIDSLLQILQFPSLTWVVQDFFQDIGGITATQWLHNLLKAHSRDSDSNLSIGEIFPSIECHTLFIPSGDRDTLRYLNRAKMSDLNPTYIRELNQLKHSLFNSLKPKLSGPAFGTLLRLLVDVANGNKFPTVPSIWSGFIKQQQQNALEDSITGYKEKISLFKTVDPPFNQDQFLELEKNSFNYANELYKQLLFGLEEAYNPGLITLHTLLKELFVLFSKENQNRIKTFNQKIYQDSFLDFQKKVEGIEIPISSKQLLSLLDSFKFESILQYKKGTDLFKLSESFDYFMGMLENDMVRVSASKQNQNTKELELLLTKAVAVVCEKYKSDMAQIKYPIPIAELKIKHKELFKESEQTFKKIASISSQENSYYAYHAMFEKNISELHDKVFGHNEMAIREKCKQETNRCVYDFKKSASQILLPVEEDYLQKNLEQYKAKSIQLFKNSLVIFDNSDAYKEEFINLQVKLEQQIDLKNKENVEQMKLIVMEYLNTVKVMMKSRYSSYWFQYSFENEAHQEADKRISQKIQSKSLRDKVIKQFIETDLKDEMNSLFKLSTLIITLSIGVLIILVISLYSRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ07</accession>
    <name>A0A8J4PZ07_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phosphoserine aminotransferase</fullName>
            <ecNumber evidence="12">2.6.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_003145</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>4-(phosphooxy)-L-threonine + 2-oxoglutarate = (R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:16573"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58452"/>
            <dbReference type="ChEBI" id="CHEBI:58538"/>
            <dbReference type="EC" id="2.6.1.52"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>O-phospho-L-serine + 2-oxoglutarate = 3-phosphooxypyruvate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:14329"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57524"/>
            <dbReference type="EC" id="2.6.1.52"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.52" evidence="12"/>
    <dbReference type="EMBL" id="AJWJ01000095">
        <property type="protein sequence ID" value="KAF2075559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ07"/>
    <dbReference type="OrthoDB" id="1703350at2759"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00197"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004648">
        <property type="term" value="F:O-phospho-L-serine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000010">
        <property type="entry name" value="Phosphoserine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000006">
        <property type="entry name" value="Phosphoserine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00160">
        <property type="entry name" value="SerC_aminotrans_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022278">
        <property type="entry name" value="Pser_aminoTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003764">
        <property type="entry name" value="PRK05355.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01364">
        <property type="entry name" value="serC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43247">
        <property type="entry name" value="PHOSPHOSERINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43247:SF1">
        <property type="entry name" value="PHOSPHOSERINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000525">
        <property type="entry name" value="SerC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 12" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0718">Serine biosynthesis</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="13">
        <location>
            <begin position="6"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2075559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="368" mass="41272" checksum="F63B3F23FD5221FA" modified="2022-05-25" version="1">MNRAHNFGAGPGSVPDEVLEQVQKELLDYKGTGKSIMEMSHRGKEFTQVIEEAKADLIKLLAIPDEYDVLFLQGGASALFGGIPMNLCNEQTDTVDFIVTGTWSKTACADSKYFCSPNKVVDMESQKFLTVIPPEQWKFSESPTYVHYCDNETVHGIEMPTDVYKHFPKSALVVCDMSSNFLSRPVDVSKFDLIFAGAQKNAGISGITIVIIKKSLLSKTKPSVPTVFNFLKKSQSNSLDNTPPTFNIYVSGLIFKWILKNGGVEGMEKLRVEKSSKLYDFIDQSNGFYKCFIDKDYRSKMNACFRILDGNQELEDKFAKQALEQKITDIKGHRSVGGLRVSLYNAIKVESVDVLISFMEKFMKENSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZC5</accession>
    <name>A0A8J4PZC5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome c oxidase subunit IV</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001749</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000043">
        <property type="protein sequence ID" value="KAF2076973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZC5"/>
    <dbReference type="OrthoDB" id="15032at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="88" mass="9782" checksum="020AA3206ABFC4AB" modified="2022-05-25" version="1">MTEHKLRYPKGFTAYPHSFHAVQGHGLPKGYVTLAGVGATLLVAGLYFGYAQATKRDFPTRSKEWKAATAAYQKEIQADPIFQLPKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZG9</accession>
    <name>A0A8J4PZG9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cytosolic regulator of adenylyl cyclase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_001833</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RICTOR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000046">
        <property type="protein sequence ID" value="KAF2076866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZG9"/>
    <dbReference type="OrthoDB" id="14744at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028268">
        <property type="entry name" value="Pianissimo_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028267">
        <property type="entry name" value="Pianissimo_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029453">
        <property type="entry name" value="Rictor_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029451">
        <property type="entry name" value="RICTOR_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029452">
        <property type="entry name" value="RICTOR_V"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13298">
        <property type="entry name" value="CYTOSOLIC REGULATOR PIANISSIMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13298:SF11">
        <property type="entry name" value="RAPAMYCIN-INSENSITIVE COMPANION OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14663">
        <property type="entry name" value="RasGEF_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14666">
        <property type="entry name" value="RICTOR_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14664">
        <property type="entry name" value="RICTOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14668">
        <property type="entry name" value="RICTOR_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01303">
        <property type="entry name" value="RasGEF_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01307">
        <property type="entry name" value="RICTOR_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01308">
        <property type="entry name" value="RICTOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01310">
        <property type="entry name" value="RICTOR_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rapamycin-insensitive companion of mTOR N-terminal" evidence="3">
        <location>
            <begin position="62"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Rapamycin-insensitive companion of mTOR middle" evidence="2">
        <location>
            <begin position="535"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="domain" description="Rapamycin-insensitive companion of mTOR" evidence="4">
        <location>
            <begin position="931"/>
            <end position="1003"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2076866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1139" mass="128977" checksum="93EF8382E251F5D7" modified="2022-05-25" version="1">MSTTTASNPIGSNNLNGVNVAPQQTASLATVVLSPDEAELKKQILNALQILDDKSLNFKIKLDYINKLVEYKKNISDLSKFGISNTTFLKSVRIFLSTPPKTMRTSALRVIRYYTTNSKFIQDVIACKIPYFITRSLERDKHSEAERVQALKLIRTIMEIDCSLVPHCVVKALVAISENQEDNFCRVCLETLCEISIRNPQISSHCGGIRTLFDAILDPFYQGIQESLLISILYILNEEKTRVYVRSKADLEIILSPLTNSFNLGVKLKGVSKEKEKEKEKDDEIAMKRWTASAKAVVTLIKSWVGIISLTSDDQGLKSIVDALRMPQLELQDKVLDSLFEIFRIAQPKNISESFRTQKAPQVNFGPDTLSDLPSRTRSLRHNLLNNYIAVLLISFIDNGLIEGLVYLGNFINREGTDPEKEQFKTISSKSTILLAELLHLSNSLLPPAQCARLQTLPSLVNSAITFRLDPRLRSRSSTMVTNLHAYSHIKASVGIFDSSMAIGLTGANKWRRIKGQDRRLDKIDDVKMKMDWHMDDVQFQQKIKDTQVLVTKDITKWNWELIFELLEGPLNNPQHLSNALKTKFIKRMLSFLRPNKKLFSTMAWTTENLKYVRVACVILEVLISHEIGFDYLKDNKTIIQISDMLRTELETYQNNKAGEKSNNNRLLNPEKVLKTMSREYFTMLGTLSSNLLGLEIMARNNIFDFIKPLAELPGRDDLSHLIMTSLDYNVNGASRTILQKILTSSSRVVRYLATKYMRFLLRSGVQDFSNWGIELLVQQLNDSDPKVSALSLNVLDEACDDYGCLEVLILLKPPLLKLGKAGKSLLLRFLSRPSGLSYLLHTNFVEQEEQLWMKEENANYVISVENAISESLTPSVWRQKEAPDQGNSGVYLPPHFFGELAKTGEGCKILKKSNNVETFVKIAKDPKANPLYRRASIFAIGQIGSSVAGFQFIEEYKVIPMLIDLAEQSSCLSIRGTCFYALGMISCIEKAQDIFNQYGWESPSDLNNRILVPKDLKKSTFLKIAPYEYKGSWAGNSLDSLPSNLFTDPIKNEIITFIGNLSSHITAEGASKNLKRLKLKHPEYFASGEMLAVVLTLLNTYKYRLGARRFIYDCFDTAIFSDDPFPYLEECNFMNNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q016</accession>
    <name>A0A8J4Q016_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002515</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000070">
        <property type="protein sequence ID" value="KAF2076162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q016"/>
    <dbReference type="OrthoDB" id="21246at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="377"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="526"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="579"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="62"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="383"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="528"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="584"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="628"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="640"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="674" mass="75466" checksum="3E361A8D2E304DA5" modified="2022-05-25" version="1">MIMDIEEEMMYSSEQEVHVPSIQPLCNNNKSSTTSHNIVNIKPTMSSSNCTSRSQSPTSQINNSPTLTPTTTTTTTTTTTTTTSTNNTNNSDDANTISPHIYRQMIDHLEQLVNQIDLSTPTVRSMVWKELYSQSLVSTYKNTKPKQIHQSLFYDKDCVSKFTPYYKSLFNTENINYHAIELLYHAFLETETKNPNYLKNISPQLSTDDNNNSTSNSNNHQNSNNNNSNSNMAVLSNPFLSSSLWGYSFSASMQQLALLAYWIPTLEKLLKPQKKKVWETCLSLQFFHQHINSKFQNVKTPHFSKIIHSWMENPLLHLNLVTIMFGSIGYLVETNVLKNEYCYPQSNISVFSSKPESLATDDEDELVDEVVAIKSPSKKQKKSSSNLSSSSTSICNSKSLKNSTGMDVPSLSPIQSPNRCSSAISSSHSSPLFVCTPESDYDLVGDNSGDEIESIHDDLSSSSTSISSFKYINLKSSVNNNNHNEEEKDTNNNTITSISSMIDNVMKKKSNISSYSIPDVFISHSKKNNSLNNNNNNNKNNNNNNNQIKNNNNNNNNNSISLLDALAATAVELSDRKDGIHEYEQDDEMEDVDIEKIDDDEESTITTQRSKIEVPAIDNNSNNNNHEDDIDQELIDDIELTSSENESTTKTSGSRKRKLNGTVKTPMSIVNLCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q091</accession>
    <name>A0A8J4Q091_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">IPT/TIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_000153</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000003">
        <property type="protein sequence ID" value="KAF2078528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q091"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052014">
        <property type="entry name" value="Dictyostelium_Tiger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31341">
        <property type="entry name" value="IPT/TIG DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035269946" description="IPT/TIG domain-containing protein" evidence="4">
        <location>
            <begin position="27"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="5">
        <location>
            <begin position="691"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="779"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="783"/>
            <end position="817"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2078528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="846" mass="92629" checksum="EC72C5925307A13E" modified="2022-05-25" version="1">MINNYLFIQCLVLFLVLISSIFNVDARSLNITKNIQITGVGANARDASISLIQGGGVPFLYVVYSTGYAYYSLDHIVNSTVGIVPMDGSESSGSVDFYRIVKYNTDDSFTFLTGKNGVYFCGPSIPSYPNIPFSEPGTTFDKMLTASTPGPNIFTLGLPYTLIQSNPILFYGTKTYTTNVPTDLTCDLSISSCFLVGSNFVDIYKVSSSFGLLTISTRTLSPYDKRINGIQNKAKNMFYYIAIGVGSFSLEGYDLDFFMRFKKDFYISTYGNFVKMAFDKSQGQLFIGTTTQIISLDWMGENLYSIETKVASNAVSMDVWSQTSIKNTGDLFITKSDNGVVVIKYDSYCPQNCNNNGYCGYSVCTCPNGGNSICNYEITGLSSRLLQDSLLVTLSGKFFEPPPSTIDIFVGSTLVPSVYVISIGYNSIKFNFDSYSESTLISFTTGGQNLTYQPNSFYPNANFNSVVQIDNILYVHGEFGSFYSIKATITSENNYESTLEISNSTTAFFTVPDLISNQLQVSFLSNDQPTSKTINLSPYFNSTLTSSDRGGDLNFVCNYYGSIPKAYLDGRIEVSVQHIEDNKYKVQIPAGVYTNITLGNGFYNISVPLEYKSLSITSVVQTDVQKLTITATDFGIDQDDVSITLSGEQLEIIDFDYTNGKIDVSLTDESIKGDLVIQARQIESISINLKPNITSITPINPSYNGDTITITGLYLSTNVEFKNKNSNSSLSCSELTSTSIHCSIPKGSDIFTIKSISEHPHQGGESLTSNVFISNYEEEPIDSSGSNETSHATSSSHESSLGSTTTTTTTTTSTAPSVEEVPEPNSAKYLQSQSVLVFLLIILLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q092</accession>
    <name>A0A8J4Q092_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002412</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000066">
        <property type="protein sequence ID" value="KAF2076297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q092"/>
    <dbReference type="OrthoDB" id="18792at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040405">
        <property type="entry name" value="DDB_G0275255-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34411">
        <property type="entry name" value="DUF6748 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035194622" evidence="1">
        <location>
            <begin position="24"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="293" mass="34024" checksum="48D2E8E21A51DFEB" modified="2022-05-25" version="1">MKISISIILFILLTSTTIIIVKGYDEQEFQFFYLEYEPKQCLTLFCPQYLATIANTGHSYNIVDIKVPSFLKKENYFPNTLNLAVYGKIVSITTESISYYNLYISDIFESLAQTETLSQVLEPLYSISFSGLDCKRSINDCPQFIISMINNNNFTNSTLINSFIEPYSSTINYFDREWYYDRLVRENDTQVLVQGEFSNDNRDFKITSSYILLENSKCQDVVSMCHESNPITVYHRDHNRCLKPQFCIDNVGPCLTKDIPNCPLGYKLSYHPSDMFGCPKYYCDPYFLPVIRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q093</accession>
    <name>A0A8J4Q093_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PWWP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006326</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000287">
        <property type="protein sequence ID" value="KAF2072356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q093"/>
    <dbReference type="OrthoDB" id="21425at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05162">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWWP" evidence="2">
        <location>
            <begin position="12"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="326"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="437"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="538"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="724"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="800"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1060"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1169"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="105"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="454"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="485"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="539"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="589"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="725"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="800"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="839"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1060"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1169"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1183"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1219"/>
            <end position="1243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1253" mass="139665" checksum="F9E4D5A7043CE072" modified="2022-05-25" version="1">MNKNKSSTIISLVWAKEPEIQYWWPAKISDEDDIDEDQESCIFDKKAPNSKLLYFFGIRSFSWVKDTHIKEFKSGANASFYEQDLPPNTIDFFKLALKEMNQLLNSNNSGGSNTNHNNNEDGDYSNKKVQHYDNQRHLLNKSDEDVVMTKEFNQDEQDGEEEDDNQYRHNQNIQYQQQQIFKLQQQRPKQQQQQNNRNTLPHYNDFEQDDEDQFEQEEQQDKIKTKKSIGTNEQYDEDDSSNLLTHLKRAREMAPSQFSLNSHKKHQENNHDMDDDQDDDEEDDDDDLKMDDQDDQSKKRKVDININNNDHLKKIDEQHQNNIIAEQSKTPSLSSTSKGEPNSPSMSAKRVLLIKPPRSPAIASRPNRPVIIGSSQNNNLSPSITQIANPINSPPTRSSQVLSSPPPLMVNKFMPTSPLLPSKLSQNVLSALMQKKNAPMPNGLPTQQPSPILQSASSPSMKSQPSPSMNPKAPITLNIPRFKKSNSPPMNNQQLNNNQQNSPSISSANNIVNNPSKMSPLTSSAIPFHIPQQFRKNVYSMSPSGSPSVGATNRMNGNNRVPNSPLLSVPMTPPLPPDAKLHTVDEDSSQPSSNNNNSVNQLQQGFPMFPFFSALPSFNSNSLVNMETSFTSPFNGKFAVKGSVETGFQIDCNIMGKSFQGFLTEMPSQPTASATGNTVPSPTSQQTTTTAANIQMNSFLQNQQAAAFFNKMGYPPNMVPPTMFNNNSNNSNLNNNNNSGKPMFNNNYRRNSVSTTEGLSFDNYQNMMGMNQWIESYRQWQDIVLKTQLLQQQQQFNQQLNNVNSPSNNNRTSPTGNGLLNVNNNVNSNSSNSNSNNSTQNTPMQTPKTIPSIQVNNKKEDTNTLIQQPISQHSDNPEFLSFKQNAFQKLEQYQKLQIINLLSKQEEAINSLLDTFEKEKAIATSNKDNNIDQDPDLVSKIQIKFKEDQEKLQQQHKDQRSKLDATHSYQATKFHREVEKQWLIQRNKGTSNGGAPLSPVGSPALTPFISPSPAPSPLLSSFTIGTSPIITPSVGPQLLKNSSISNNGIMLMKDTCLSTSSLSTPLSSSLNNNNNNNNNSVVNNSNNNSGNSTLNNNNNTNNNDSSNNNNTNNNSKNLTNSTSSQPPPLSLSSNSPPRSPNDKKLNSIINEKLQTSLSNNDKDKVLLTKSTLSSSKSSISSEEQQEDEEEQEDGEIILLNEKDDFDDDIIVSDKEFEKSSSSSSSSSKNNNKPSSSSTTKSSSANIGITLHVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q108</accession>
    <name>A0A8J4Q108_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_002835</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000083">
        <property type="protein sequence ID" value="KAF2075849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q108"/>
    <dbReference type="OrthoDB" id="1932312at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051350">
        <property type="entry name" value="WD_repeat-ST_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22838">
        <property type="entry name" value="WD REPEAT PROTEIN 26-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22838:SF4">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="449"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="84"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="92"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2075849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="487" mass="54544" checksum="A7269D47E8B8EFA5" modified="2022-05-25" version="1">MDTKIQNNIVGDILFVDENFISNRIACIERTTDWSSDAYLQCRKSLFDQKIKNKKFILRDIGDRRDGYIQKRFQLLENKYKKQQTLSSKKPTTTTTTTTTTTTTTTNNIKNSPNISNIQLTPSTLTPPIASSPSTPLATKQPKQVGLEDFSFTGMHHIFAEHKKAITRIKFANDSLDLLAFSSEDGTISIAKVMNKPPQIIATLKSNSNSGTTTTIPSPIIDFVWCNNNNSIVSISMDGIMNIWKENYQLFTKPIKDIGSCLCLVAHPTKTNLILLSESRGPLLKIFDLEKMEITCKIKTATPVVSMQFEAKGNYVFLGEAKGVLSIYKFEHTVPQHVLSMVSRTTISATPITSVSVHYWHQSNQQQMLSVLTNSQDSLMRLFMVKSLSTGTIVLFREYKIPIRTKLIKNTFAPISKQREGVYLMTGSENGVVYIYNANKKDKSCINELMGHASATIDLSWNSDESLLATADASGTVILWSRKYLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1E5</accession>
    <name>A0A8J4Q1E5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycosyl hydrolase family 13 catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000028">
        <property type="protein sequence ID" value="KAF2077465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1E5"/>
    <dbReference type="OrthoDB" id="1740265at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11347">
        <property type="entry name" value="AmyAc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47786">
        <property type="entry name" value="ALPHA-1,4-GLUCAN:MALTOSE-1-PHOSPHATE MALTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47786:SF2">
        <property type="entry name" value="GLYCOSYL HYDROLASE FAMILY 13 CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="1">
        <location>
            <begin position="20"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="498" mass="57166" checksum="D7AF29848060EF5F" modified="2022-05-25" version="1">MCTRAQSNIPNGGMMMYELSTRPWLYNLSQQYNMDIVTLNDIPMQEFQKIKDMGFDMVWMMGVWELGQYGLNYDQTNQGLLQHYAQVLPGYTQSDIIGSPYAVTNYTLNTQLGFEQDLINFRKKLNAMGLLLMLDFVPNHTAVDCEWTTTEMDYYIRAPKNIQPPYDPNTYLPSGIAYGSAGWGGGWPDTAQLNIWNPDTMAARINELLTVASFADGIRCDMAYLLLNSLFSQNWGTQLQSWGYTQPDQEWWEIAIDKVKSVYPNIIFLAEVYHPWEEQLQQVGFDYTYDKMLHDYLGGGNMDQVRDWISGHSINFAKHSAHFISNHDEPRGANFFGSWWRSDAAALITYTLPGLRFFWWGDFQGYQNQLDVHLRREESEPAVDTAMVFYQNLTSIISDPVFKFGTFQYLNVSNDDSGSLVAYKWSYQGEKRLCVLNFTGQDASGNIVLDDAEPLNGNDTIAVTDLLSSTTYYRSAEEMRTSGLFVIINSWYGQIFKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1U6</accession>
    <name>A0A8J4Q1U6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_000967</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000021">
        <property type="protein sequence ID" value="KAF2077720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1U6"/>
    <dbReference type="OrthoDB" id="1666796at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF111">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="338"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="409"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="442"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="495"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="528"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="567"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="600"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2077720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="636" mass="72442" checksum="60DB5C1E9FF08C28" modified="2022-05-25" version="1">MMMKRSTTSLKMLLSSLAVLFIVFIGCSDAVFYLPGMAAKDFAVGALIPLKVNKISSVHTQIPYKYYSLESVCKPERIVDDTENLGEILMGDRIENSAYEIPFLEDEGECKVLNYFAKDCNITVGERALKQLQERIEADYKVHWLVDGLPVREYEKSAIDTGFRLGFKEKSGGDSKSTLYYVYNHVDIILMYHQKQGSDTSYIVGFEVEPKSIAYDTSSYDVSNTYLCPKSKGVAQSVSSKSESILWTYSVSFKESKVLWNKRWDVYFETGDSSFHWFSIVNSLMIVFILTIMVAMIMMRALRADFRKYNAIDAEDSEETGWKMIHGDIFRPPARPMFLSVLIGSGVQVFFMTVVTMVFAVLGFLSPAAIGSLATALIVLFVFMAMFAGYFSTRAFMFFKGKNWKKNTLYTAFAFPGIVFGVFFIINMFLRGSGSSAAVGPGSFAAIIGMWFGISVPLVFWGSYFASKKPVPEDPVRTNQIPRQIPDQIWYMNPYLSVLMGGILPFGAVFIELYFILSSLWNNQFYYIFSFLFIVLIILIFTSAEISIVMCYFQLCAEDYHWWWRSFLVSGSSAIYVFFYSISFFGQLQITKFVSVLLYFSYSLIMSLAFFVLTGSIGFFSCYWFVRKIYSSIHIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2A5</accession>
    <name>A0A8J4Q2A5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001855</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000047">
        <property type="protein sequence ID" value="KAF2076827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2A5"/>
    <dbReference type="OrthoDB" id="6359816at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006516">
        <property type="term" value="P:glycoprotein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1020">
        <property type="entry name" value="Peptide N glycanase, PAW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038680">
        <property type="entry name" value="PAW_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006588">
        <property type="entry name" value="Peptide_N_glycanase_PAW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24413">
        <property type="entry name" value="SPECKLE-TYPE POZ PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04721">
        <property type="entry name" value="PAW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="409"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="245"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="560" mass="65045" checksum="220F77BA810FB3D2" modified="2022-05-25" version="1">MGTLENYFKLNSNNQIYTPYKLDFETKSSLHEKNSIQEIIQDRGLYSTRLVYDSIKNRYMLSQGRRNDLQLSSIHPGYYKQSGITYKNESKFKMSFLHTSQGSEKAFIHWRWNFDDSLSICRAMVVINYALFNRDTCIDWYISTERDIEFNLDNPSPNWKRIPLIKIYASKIFIEKQIDLSLIVSGSSHLNLVVCLSNSAACEKAQLFRKCSSPQANENNPNPMPMSKFPFPFMVDIDLKPRLDNNNNSNISNSSNEQQQQKDHSISSGDLFLEQEFEKLTFSSPVLNSHPMEWSTDRVCQWLFDNGLFTLCNYFHETKKRGSDIFSFNVSNQSNIVKSKWEVAMSNLYSYLGQSYSNPDREKYVEMCGNDPYRYNSILKIYQQDQTPHLLSITLPKASDFILNDQQTSDFQIHIDSGNDVGSRILYVHKSVLTAWSLYFTTLFSSTTFNENKTNKTIFKSNTMDYDSLVVVLNIMYTGAKQLVDQIKFESISSLFNLYIISQSLMITSLMDIFEKSLIWSVDELNCDDILNKSISETCHAVNLYKFCKFYHKLPKLNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2W5</accession>
    <name>A0A8J4Q2W5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005540</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000223">
        <property type="protein sequence ID" value="KAF2073151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2W5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="671"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="680"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1217" mass="139377" checksum="9A70EDE0C4F566BE" modified="2022-05-25" version="1">MKRKTTTTTTTTTTASITTSKVKEESGDAIEQPETVINGDLVWNTPSLYDELNVKDIFMYRVTTEKLVNNNHIQIPHSNISRLQIVVTNDRLLQQQYRNKGSPAPIIPSSISYLEIHLDIGMISRDYMSVINFIKSLEIPPTVTSLAITSSTWKESLEKIVYGILESDKLITKKRKTSKSSKEPIYDYFVPPTVKTLSIPWTTKDIKEFIPPTVLHLVLQDQPTSISNLNVPSTVEHVEFESLEIKFQPLSLLNLPDGVKTVTTGSFYSKHHVQHMHYRFNMLYDTSVLHTILMRYFSRASESLAKDNICIFSSYRTVTNENPKTVIFLTGTTSAFPPSTRSVYFPPFYNYPLTLPVGVEYVRLGNYITHFVLPPTLKYLALGEDFHDFLYGNRYIPTSTIYLEIYIGTSIQYKGYIPKSVKYLKINSIPGKTNKLKDSSFIFLPKSLIHVDIDPMYVDRIKMLEEDDYYQDNRKLFYTNYTNLKSLVLPDSFNHYIPMLPSNITQLTIGRGFDQPLDSNLIPPSVTSIVFNNKNLLLLKNLPSTVKQLEYSYPTSVDQVDQFKRVFLNIDIKQRLDIEPKLEGGLIITLGQVKIISDQPKPMVLDTLKIHFYYRAYSSTINNLLDPSRRGGDVGKKYIFSPREHINIDILPFVKDVTFDYEPNKGFVLKPRDENEKSNLENNNNNKNNNIRLTEPIKPGSDLFFYIWKNRYLRQIISERLISPNDELVQVERPIMGSNTLRIDKQLALSRVVVSPNISKIVMFTHVLKGLTKTIPKKIFNVLEYQYYFYKKDKPIPQGTKYVFWSLNQLIPIGCIPDSVVSIVFCNSYNQIVLENSIPDSVQHIEFGYNFAQSLEYVYLPPKLVYLGFERDIAIIPNKLPGTLKQVVFQNLGRLAYNSLHLLPPSVERLGVTIEKDYTHIPLSIKYLKIFHPRHRVYFNRGTLSIPPSVKYVTIPKGPIFTIEVAKASSAKPSQQQQHQQQQPKIPYLGDNIPQGVNIHLRCTEVLLRPNMLIGNIKTIILDGLYNSTLVPGSIPNTVETLYIKNDIYDQPILPNVLPSGLKKLVFGFRFNQKISPGILSSLSDLEELEFAESFDQTLDLQLLPPNLKKLSFKRGFYDQSLLDWIPSSVNNLTIGSLRPHIRNMNCFPIEKLPTTITHLSLGDNQNIESPNLIPPHIKHLRLGPCKNYDGCVPQSVEHLEISMFHNYPLHLLLPPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3P9</accession>
    <name>A0A8J4Q3P9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000381</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000007">
        <property type="protein sequence ID" value="KAF2078289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3P9"/>
    <dbReference type="OrthoDB" id="659430at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2078289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="69" mass="7712" checksum="56BE224F10E9BB34" modified="2022-05-25" version="1">MSNTIGHIKGAIPNGVEYLDFGDKFNQRLPTSLKVLKLGEDFNQDLTGVFPSSLIELVLIMRCKPIFKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3V3</accession>
    <name>A0A8J4Q3V3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000959</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000021">
        <property type="protein sequence ID" value="KAF2077712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3V3"/>
    <dbReference type="OrthoDB" id="407198at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="142" mass="16414" checksum="1AC6E19F9DCB1DC4" modified="2022-05-25" version="1">MNSITANIDGACKGNPGKGGWGYVVSDGFRKNGGKKYTTNGEMELTALYECLLYLSTKNYKHMTVHGDAKWIMDALNKRWICKWELNDWKKYNKKEVAHKHIWKNISNILYGFRNKSVVVFKWISRDKNKEADAEANKGVYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3X4</accession>
    <name>A0A8J4Q3X4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_000414</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Aromatic compound metabolism; naphthalene degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000007">
        <property type="protein sequence ID" value="KAF2078322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3X4"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07092">
        <property type="entry name" value="ALDH_ABALDH-YdcW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000010">
        <property type="entry name" value="Gamma-aminobutyraldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000007">
        <property type="entry name" value="NAD/NADP-dependent betaine aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015657">
        <property type="entry name" value="Aminobutyraldehyde_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010000">
        <property type="entry name" value="PRK13473.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0058">Aromatic hydrocarbons catabolism</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="7">
        <location>
            <begin position="15"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2078322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="476" mass="51218" checksum="5DA200C26BC6E404" modified="2022-05-25" version="1">MTLKVLQHFINGEYVSAKGDSTFDLVSPVDGKVYAKSPNATDAEIDAAYAAAREAFKIWGRSTPSTRQKALLKLADAVESNAQRLIDAQSTCTGQLKHMIASEEINGSVDQIRFFAGAARLLEGCSTGEYLDGLTSSIRREPLGVVGQVTPWNYPLMMAVWKIAPALAAGNTVVLKPSDTTPQSTLILAELAAPFFPKGAFNVVLGKAEVGSKLVSHKTPALVSITGSVRAGIAVATCAAANLTRSHLELGGKAPVLIFEDADIDKAVESITLAGFFNAGQDCTSATRVVVSEAVHDKFLSKLVDSAKKTRFGEPDDKDALYGPLNNANQMKHVKSFFEKLPPHAKVECGGKQANRPGFYFEPTVISGLKQQDDAIQNEIFGPVITVQTFKDEADAIEKANGVEFGLASSVWTKDHARATRLSRDLDFGTVWINTHIPITAEMPHGGFKHSGYGKDLSTYGLEDYTRIKHVMSSNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q542</accession>
    <name>A0A8J4Q542_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Histone deacetylase</fullName>
            <ecNumber evidence="2 10">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_004596</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the histone deacetylase family. HD Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="2 10"/>
    <dbReference type="EMBL" id="AJWJ01000165">
        <property type="protein sequence ID" value="KAF2074111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q542"/>
    <dbReference type="OrthoDB" id="1918432at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000001">
        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003084">
        <property type="entry name" value="HDAC_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF10">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037913">
        <property type="entry name" value="His_deacetylse_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01271">
        <property type="entry name" value="HISDACETLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="15">
        <location>
            <begin position="23"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="372"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="396"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="427"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2074111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="482" mass="55285" checksum="FD0A16F9825F92CB" modified="2022-05-25" version="1">MSRRKVAYFYDNEVGNHFYGANHPMKPHRMRMTHNLILNYGIYKKMQIFRPRKATDLELTNFHSDDYIKFLKLVTPDNMNDYAKEIAKFNVREDCPVFDGMYNFCQISSGGSIGCAVKINSGESDVAVNWAGGLHHAKKSEASGFCYTNDIVLAILELLKHHQRVLYIDIDIHHGDGVEEAFYTTDRVMTVSFHKFGDYFPGTGDVKDLGAGKGKNYSLNFPLKDGIDDESYQSIFKPIMQAVMDFYRPGAVVLQCGADSLTGDRLGCFNLTLRGHAQCVEFMKKFNVPLIVLGGGGYTIKNVARCWTYETSILVDSELTDELPYNDYLEYYGPEYRLHITPNNMENQNSKDYLEKLKINLLEILRNLNHAPSAPQTEIPPDAFNYSDDEEDEDPDIRVSEAERDKRISHPAELSDSEDEDGRKNESNGLEATPTQRRTSRNQVPITAYDKEKPIYDIKFQRRQQFDEDVEMKLKKKKKKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q5D5</accession>
    <name>A0A8J4Q5D5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_004435</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000156">
        <property type="protein sequence ID" value="KAF2074261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q5D5"/>
    <dbReference type="OrthoDB" id="10259130at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000039">
        <property type="entry name" value="Cysteine proteinase 2, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0458">Lysosome</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035209017" evidence="9">
        <location>
            <begin position="20"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="11">
        <location>
            <begin position="34"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="10">
        <location>
            <begin position="117"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2074261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="334" mass="37366" checksum="1B9B3D296C380657" modified="2022-05-25" version="1">MLKKLTLIALMVMVALVAAKHPHRYRESHYKEEFVKWMSHRNALYSSVDFHDKYENFKSNYDFIANWNDKQSSTILGLNDFADLSNKEYRETYLGTTYDVSDLEESTRLNQIFVGDVPNHLDWRVQGAVSHVKDQGRCGSCWSFSTVGAIEGSNFVKTGVMTTLSEQQLVDCSKTYGNHGCNGGLMEPSFQYVIDNGGLMTETNYPYTATDKAACKFDASKGPITTISAYNTTVRGSEDSLLQAVQLNPIAVAIDAGQRSFQLYKSGVYYDSNCSSYRLNHAVLLVGYGVEHTTNSPYWIVKNSWNTVWGNQGYIMMAKGSNHCGIASMASYSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q6R3</accession>
    <name>A0A8J4Q6R3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HEAT repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003496</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000110">
        <property type="protein sequence ID" value="KAF2075191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q6R3"/>
    <dbReference type="OrthoDB" id="16167at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2075191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="214" mass="24884" checksum="0DD1E9879D821DA0" modified="2022-05-25" version="1">MSNFETINNIIIESFIQIKDSISQDSLMYMAGVIEGSESEEELRDQIKIFCTDFDITFDNDSDMDNAVDHLISQLKKKGIIEFSLATKPKSYLVCNVSNELSLDDPNLTMEQYLQFTHSEDPKVRLSVLRTMCPCKVKADRDLLWDRIMQMSTDTDPKVRYQAMHNLCDGSPAWREESVIKTLESMHNDTDPKIRRRIHNILTHYKHTGKWNIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q8L6</accession>
    <name>A0A8J4Q8L6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCAAT-binding factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001888</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000048">
        <property type="protein sequence ID" value="KAF2076811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q8L6"/>
    <dbReference type="OrthoDB" id="28947at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040155">
        <property type="entry name" value="CEBPZ/Mak21-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12048">
        <property type="entry name" value="CCAAT-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12048:SF0">
        <property type="entry name" value="CCAAT_ENHANCER-BINDING PROTEIN ZETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCAAT-binding factor" evidence="3">
        <location>
            <begin position="461"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="681"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="733"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="115"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="743"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="775"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="792"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="828"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="863"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="897"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="949"/>
            <end position="958"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2076811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="989" mass="112793" checksum="D83798FF892185C3" modified="2022-05-25" version="1">MVETKKSNNKPTTANSNKKGGKPAATVNNNKQQPKKQQQPQQPQQQSEPVKKNTRATDTATKKYFGNSKKSEKPATTPVGADKSTTKNTTATTTNKNTKNNTTTKQSNEKKTKQPTKSTTTAPTTDSDNIKLNYGTNVENRKHPLYTKAEEAFKQKLDHFQTEFKKSSSRDDQWKEKIQSTGTIRDRISAITLLVQKAPMYRISSLDILLNLAAKKSEREREFAVNSLKDLFINSLLPDSKLKRFYERIPNINEKTVKQEELSQWYFEDMLKARYQAFIGLLEVLSKDTVARIRIIAAATIQYLLMKKPEQEEVLLTLLVNKLGDEDATNVSKITNFLVKLLEAHPGMKNVIIREIEMFLYRANNAAKAQYNALFFLNKIQVLAEKDSTSVTELNEMSNRLINLYFTFFTTKSQAGSSCISLILFGIRKAYANVKDTTKLEPKQLDALFKSLRKNSINKVIQTLCLLFEIKLHTPEISDEFYQNLYYTLNRIEHLSQFDQTSFLNLIFRAIKHDDDINRSKAMIKRLLQFSFYQKVSFSASCLILLSELIRFNNSFGEMISVAQVPSIEEEQEQEQENQEEKFNIKTYDCYHNNPKIVKAETTCLWEINLYKDHDHPTVSGFANDLLNARPIKFQGNPTGMFTLHAFLEKFVLARPRAKNAKGSQLAVTKSGIKSKYIDGEEDEDQDAQQDNVPEIDASNPSDKFLQTFGDISSAVPQKSKKKIEFEKDEDEIDKALGYNTEADQDEDFDMDEMEEYSYSDLEDEDFEDEEVEFETPKQKVDKKPTKKVQNDSDDDQDVDQDDEQDDGEDFNIDEQDLYNDDLLGSIYDEDDADVDDLEIDDDLGSDDEEEEEEEVENDIPMNKKSSGKQQQKQQAQSKNKKNQKQEEQETNIDTPDFGDEDDDDDQEDDDDEGFVPADSDDELAGDSDDDNGFMDADDFAALLDSSGKLPQSKNQFLSKKKPVAASKNTKQTFSKKAASGAKGKRKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4QBC5</accession>
    <name>A0A8J4QBC5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_000196</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000004">
        <property type="protein sequence ID" value="KAF2078446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="19174at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="1072"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="1143"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="1176"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="209"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="351"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="744"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="989"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1301"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="369"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="777"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="798"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="990"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1000"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1301"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1315"/>
            <end position="1334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2078446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1334" mass="148184" checksum="06762AEB99E11CB1" modified="2022-05-25" version="1">MSSKSALLEKTFIANVGELFKYLDSLTVPKVFSRSIEQANLPKSKSNSNLLKTTSNATTTAYFIYQDQMLPCLNALVHLKTIKQFSIAEIFRERMSKTKDLLTEIKIQHYIGLFQFYLKTLDVLYEKRKGLGEISNQVINHLSTYIVDPDSCVVGLIPSDDLMNKLSRDYDFLKLDEYNVNSNNSGGGSGSGSSAIGILGGVSNMVIGNNSNNNNNNNNNNNNSNSSSDDLRSTSDRDSVHLHSFLSTTISTSRQSLLINIINWSALDDVGQIAKSVRRYASILRGVDKIKKGDVVIVKFNQYNPKKNFIGFKFNPSTSGGGNTNTSGGGVSGVADNISISSSSASIISASTLNNNNSSHDNQRHSTTSSNSNNNNSNNNSNNNNNSLKTLDEYIEKRLYKPKAQFARYTGERDDYTIKVYFSSEETETVVVGIEDVLPLNRYTIESLRDLKLVEKMIQQPSERFFAQILEDSKQKTINYLESLGVDLPFLHNASQNCSDIFEVEKLRPMYKTLLSIYKNPEHQFYKDLEKYINEKIETYLVQLDHRLLFVPKYESTIQDLKCGHYISISKSKIRQYLGECEMAQDRLHMETKLLMNGVRKEEITEWWLKRIELRTNEFLSERGYNFQALPENYIETPPSSIEDIISNLMLVQRILDPREQVIEIITVDENGTATTNDDQDDISNISTTANTIIPTNNNSDNNNNNNSTTTTVNGKIITLDEDLDDSCISSASEYVASVVAAANNNNNNNNEDDDCNDSSSSNSAGLVRSPPKQVINNNSNNGSGSTTSSRSSPVSSFTLPQQSTSLPTNIDDLTKSDQISNSLLSNASLSHFKQSEEKDSTTTITTATTIIEQPTSASKLSSSTSKFINSPVQLIKELREEIYPKINEFMKKLHFVLCEQLDLPFSQFNTKKITQLLDHKKVHMDSQTVEWLEGILSDLDYLAEMQRLEDSQEGGSMHIVPIGGLVMNTFARLENELNTAVELWHVPSDSDSTSSGDENTNGATNAINTSNASNSLDGSTTLTMKVTKKPPPTLDQDQRTVLHLSIATDDPEQVEKVLETDSHLINDYDVNGWTPLHTACYSGNADICKLLLSIDEIDVTKRNKDGASVLHYLVRHPLTEKRKEVILSIVGKGLDISCGSRHGETPLHSTSFRGHNDVVHFLLEHGANPNAITAGKETSLHYAVTAGRANVVSTLLLYGAQWNISSKRRGTPLELAKNAKLQNIVSILENGPDVDNFSWRKKHKEKILNNQEVKLTYASPTSTTTTTTPVVSNGIGTLKRQCKISIGGSRRLTLHINSNSNDNDIFNNNNNNSESTATTTTPLKDCNNSNIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UNW0</accession>
    <name>A0A8J4UNW0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010229</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000982">
        <property type="protein sequence ID" value="KAF2068446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UNW0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="101" mass="12001" checksum="59EE534FD2D0781A" modified="2022-05-25" version="1">MNLSPNVESYRLKLVSQSAHEIDFSKINRYASKKLQSPRRLQVVFLKWVYTPNLEYLSTNRLETNVPASVTELDIRDLDDYDLEFCHHISKIFMLHCIHLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPR8</accession>
    <name>A0A8J4UPR8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">USP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_009082</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000633">
        <property type="protein sequence ID" value="KAF2069601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="289038at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056850">
        <property type="entry name" value="ARM_UBP34_24_USP9X_Y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF827">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25010">
        <property type="entry name" value="ARM_UBP24_USP9X-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="5">
        <location>
            <begin position="2005"/>
            <end position="2396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="162"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="454"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="977"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1193"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1315"/>
            <end position="1352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1514"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1689"/>
            <end position="1709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1824"/>
            <end position="1853"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="2275"/>
            <end position="2303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="2653"/>
            <end position="2679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="69"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="181"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="463"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="517"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="532"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="546"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1200"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1829"/>
            <end position="1845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="2281"/>
            <end position="2297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="2653"/>
            <end position="2678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2069601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="3209" mass="365842" checksum="8BEB3AFF357C1385" modified="2022-05-25" version="1">MIETKITTTDNSGDQVKQQEPQVIVTDNKENKENKEIKENGDVVVVNNSSSSIGCEGSNVKKSSSGAETNVNVSPTTTSVSSASDSSTNNNNNNNNPIKTNPSTPFTSPSTLDNNSSKKQIKDTIDFSKYYRDIESSLRNETSPISQLENLIQIMNNNNKFRKDSNSQENLLETPTSTPTTSPPSTPSSPPIIIDSDQMHSFLQLWLPKYIISVLSFKSLSTDIKPVIIEFLKTAIQTTVDYMSVSDYNDLWASLCRILYVSHPFYSPDRFPINKDNVVDMPNILKPKIEVPSLFYFQNVVVLVESNYLNKLLTLLSQKPPLFKLKLAIKSLYFIREYFTISFLNGYLIKLKESLFNYLLDLFYEDLPPIEKENYKEIYQSFQHLLAMIVSQPEIARISQEFDLNRALKCFQSVNLEKKLRGIRYIKKESAKCIKKESDRSKLTNFLKTKTKKLSSALPPPSITTTIVNNNNNTTTTTATSDNNSNAGNNSNSSNSSNQNNSNQTNDSNIKITVTQTDNNNTETISNSNNTKVNINHSPTNSATYSNVNNSNSNSNNNNNNNNTNNNNQNNNQNNPQLLYEWIIKNEIIENCCKLSDQPDIMRHCIPVMIFLTQYGQFNHGFIDLLWSSSLGKHESVKNMIFMAILEIVSHLCFESVEYLYNSICKTINILRVSEFDNEFLSFLFNFATLSIQHSENFSGSNKKLFGLEIFWRLSTQQCEDASERELYQSLCIRSRNQFISLLKLQECTSMRTYYLECAIENLKNNTAIVESLDLIINIIDPKKQKSYIEILEQKYKIFDLFFKEISNYKKVSLEKSKLNIGNSNSNNNNADQNHQQLDSDFNNTVFVGQFPHLTQIHVRIQFLEFILSQSNFMLNIQQVDILWDSLVTNALTIEERESCFLWLKNTKISGQRSTLSESVVKHLFLEKMSNMDWYHLGPSSFSVYERFFFFLNEKAGKLKKIQSSLFNDFSNQLNGNNSNVNNNNSNGNNNNNSNGNSNLSTSTKGSTKSSPMLSSSPSLGSTNSPSISPSSSPLLSSHQHHQQIQQEKQAAATTSNSNSNSNNTTSSAPSSSQPQNNNNNNSNFNNDFSPSDYFIASIDLMGIKYLWSIALDSISLELSDLAINTLINLYQSGEGQKSSKYKEEFIKICLENITKNLDTMSSPTPSIARDSAINRVCRALTMLKIFKNEPKKDPNTLINSNSNNSNNNNNNKAKIFSFQQPSNLPNDRPLTYLVKFDRGDAYTIGDIYGTDTLRMFKRKVANFYGNPKPSVKSLKIYVGDKEFKDDDKAMEDYKIFDSNGLQIYPLIVKRILNNNTTNSNSNGQDKKEKKSLTMTTSTNKDQKKKNDSISNEQIESLQLEEDDEEIDVLDDTPIASMVIESLQHQLGGFGLIGDLLLRKVQTGNPNSTKSSKMNNLKNKNEDLVANLLTKTLYFEKLFNLLNLDDPLIASEKAWDVLSDLPFSDRLLKEAKSLRDDLPLLFPSHSVYRTLHSLKLLEHLMIGNNNNNSLDYQNISNNNDKNSNNSKLNNQQPFTLGNNNNNIEISPEEFKNNFVNSKQGIEYILNIFTKTPISCQKGTIKLFGSILKLLSFLTLQSFNPTLEKPIKTFGMQKKNLKNQESMLKFYNVEFLLKLFNLISTVVIQKDNSNQNNNHLSEDIHILIHYGLELFTTIILTTIDSVKSQQQSDNTVSQSSSSSSLSSTTSFTPSCSSSIKILKILKWFDWEQWLRLVLLECSDRNIQIKTSNALLNIATNFKIYDPSIQIDLSLYFLSILLSFLPQINQYYSTAEQYFCLLSELLNIGYPSNSSIVAAAGHSPLPLLDNHKRTSSSTSSSSSSSPHSTSSLDLQEQHPKDKDESLDFVKHLLSKLIHELKNIQAREVLNVNLKDHILNGFLNVIRNLVKRRPSLKQLCQDKELVEFLFTKCLFQTPVANDHHGSLDQPPLCKVKESRISCFRLLTELCRNDHTIFYKVVDLITNEIDSIETVDDWNYSPLEKEKSVFGYVGLKNQGATCYMNSILQQLYYSPLCKGITSLSLPPLINPSVSQEFQISSNNSNSNSKDNTDKTMIEQQKRNEDIILLTQLQLLFVYLQESSKKYYDPYFFCRSIKSYNGAPINLSIQMDAYEFLFTLLEKLEDALKKLAPQDKDILKETFGGTLANQIISKECTHRSTREEPFFSVSVDIKNKKSIIDSFYAFIDEDNLVGDNKYKCESCDSRVDAIKRTLIDRLPNTLVVQLKRFEYDLDNMRNNKLNDYIEFPTLLDIEPFTLDFAEKKDKKNDNNNNNNDNSNNNGTDNNEIPKEKEKEKQNYKYILTGIILHQGTADYGHYISLIRDKNGYWFELNDTLVQPYDWNRIPIDCFGGFDEVRELDKDTQKYVNVKRPKTCNAYMLFYTKYQDYSCNTLVKSPSSSTTSTTSTISKEPLNIVWKENHTYLKEKFLFDLEFGSFVWDIVNLYHNPTFNPLTPYPIILPNDNSLEYKESIFASIKLSTKFLIDIYSHSKEKRLIDVWGYHIKRLLRESVDGAEWFLNFFTENKLLLKSLLVSCVFDNTKSVLVDIITYAVLIRKNIEPANREKCIPSILNLCNTLMTIVKCYKDHSRRAVQKLFQLLINIVQHAENEREYLLEKGIISKLIDTFIGTYILSTPGSYSSSSPFSSGMNSNSSSNNNGNGNQNNNINTLNQLPEQNKILDLISFLVRFSQVSNESVLQPSQLNNYPSEKDRSKPRIHIFRNNYLTDDTSIAAIIPSTSSTRTNVGFNQLPLTYSSNTIYTFSESTFISILSKAFLIKAIKENGWFDSIRSMIKHLCWENKMISSHFLNVIKETLLKSHAQLYINILSCLNCLLEMQDSIATWRIENFMSSLIKLPDNMIYNQEGHDVFLIYLGKITGTTNLSSSKFDVSHYHTINTTEFTMFVKKNKDAINVNDAEKECEELYNSFQARLDEKLQETSEVDRLRDKIESDAVTIRKLKASNEAVIKTAAAQITKLEYDITNLKDQNLTVVNDRLNKKEMELTDLATQSREEIRNIQERFNERGSEIKKLEIRNQEEVQALQTELETANSVNSGFDQHVREQIDELEMVNNEKLAYFKKNRELEAEILFLRSTHSGPTSPIRSNNNINLSEFINNNNNSSDNVNSNISNAPIVNPLSTSKLLSRNVSYSPSSLSKERINERGINHLKQILKEKGKMINPCSIAISLYEARSGNVSDSDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UR86</accession>
    <name>A0A8J4UR86_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006904</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000339">
        <property type="protein sequence ID" value="KAF2071776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UR86"/>
    <dbReference type="OrthoDB" id="10577945at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="172" mass="20040" checksum="80CF30C009724D9E" modified="2022-05-25" version="1">MKDSIAWLLERDKQKDKDIKWLQQKINDIAMGNGEYRKRVYNKHSTKSEQEKIFIALTCASAMSNYYLEVILMPLLQIKYGSVKKPSCFIWRKFRDIFDTDNEFKSYASTNHGIDLVEVRRLKKDVDYGEDDISIADILKAVEDYKESLPSHKAIVESLLAQVKADILEINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4URH7</accession>
    <name>A0A8J4URH7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006441</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000297">
        <property type="protein sequence ID" value="KAF2072241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4URH7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="626"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="650" mass="76301" checksum="49757347A6369E28" modified="2022-05-25" version="1">MIIDSYRLVFGNKFLASHIFNIVHQLQINKYPLKYNDIVDVGWMIKYGHVGLAKEKINDHSHLYVSHEDLFSIVANTDTKMFIYLFEKYKYYALQYIDNGRKSLKENQQVIEYLLNNGYVVYKKRTPCPSISSCSLDRDKAVESMVGITETDFIFTLESGFDMWSSCIDRSGAEFFKIWKAWSRRIRITPNTILFHLSRDIRDDDNDVDLYNDDDYVIDISRTITKAACVSSSLIILGFLIDKGLKAEVQYAIDEYEDFFGDRMAETPLSHEECHVVVKMVRSDVKWIQDVLLLCSFYGYAENFNSLYPSLKQLLLDPKKREQILFECVKYKNYHMIPVLASQGIFFSDYIDGDMLFDMCNHFSGAFSHLLEYIDRAITNTVAPEARLDTCTEFLKCAIDHDDFVGVKFIFNKYKFESLDENVIKTLSFLNNLAIIDYINKNRSLCFTETALNNNYMHTLFSNLFNQVLISPLNIPLIEYLVKENCINFKDFNCKLETQAFFNPDFSGYIQNTYHFHEFVYLIDHNNYSSIDPFIKLFINDTNWRKYLVYIYRNQHKFQMKPSFQSIFDHIVINLNQKNLQKSKIKLKALVRRYGCVLNDNHYQHLVQYTDGLTLFGIVPHSIFVSNNDQPPPQQYQNDTSSRPLKKKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4USC7</accession>
    <name>A0A8J4USC7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carbohydrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000202">
        <property type="protein sequence ID" value="KAF2073471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4USC7"/>
    <dbReference type="OrthoDB" id="19902at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040310">
        <property type="entry name" value="DDB_G0292248"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31648">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25544">
        <property type="entry name" value="Ependymin_amoebozoa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035246615" description="Carbohydrate binding domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="24218" checksum="E726E15272120BDC" modified="2022-05-25" version="1">MKFLTIALLVLLSVSASLAGNCFQTTGYHTSFTATGFNSILPSNNGVPLSFYEAGTINVDFAQQQMAVTFDILTTDSQVLGLLYAFAANQTMYVVNIDDNGSTCTKLPLNVPIPTSFPNVTADLGPINIGKFQTEVLEIVENGSQQKVLYDLQNCALVSSYMLNEPSQPGVTTVNFLNFESTPAPISLPSICTNPSMSIKSNRHNSKSSAKTPKSIQQLLRPFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4USM7</accession>
    <name>A0A8J4USM7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Spindle pole body component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_004895</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TUBGCP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000182">
        <property type="protein sequence ID" value="KAF2073801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4USM7"/>
    <dbReference type="OrthoDB" id="2192946at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF13">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="8">
        <location>
            <begin position="338"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="7">
        <location>
            <begin position="639"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="732"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1006"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1142"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="35"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="77"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="168"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="201"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="220"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1006"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1032"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1047"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1083"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1142"/>
            <end position="1157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2073801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1194" mass="135682" checksum="64DB263D032CDD6C" modified="2022-05-25" version="1">MQQQQNQQPKQSNTFSTKTINNNVTESSTVKRDTGSSSSGRPTTTTTTSSSRPNPTSATTTTSSRSNTMNQAPIRQTTGPSTTTATITTAPIGTNATPTRRSTPISSSSSSSSTATYNSNSNNTTTTRVKVSNNTTSSSSNSISYPDLKRESSPPITTTTGYIKPLPTTTTTTTTTSSSSSNIPQHQHQQNKTESPNITSDNEDENENDDVSLSPRTLKKLQDMQKKHSNLDDPLPSYLKPQTNSSPIISDNNNIHHNSGGNSNSKQTAKLSSIPQFSNWIKERPFLNNGYLSNTSHNYIKLEQNVKSKFVTLPGKYEKSKINLADQSNFEQENLLMEDLLSAMMGIEGEVIHLMTEAEINSNSEDIGGRKLIHFKVEGYVDTSNSLLIQRMLPICSYYSFINDFVNSRYRYEWGLINHSLCACINVLMKEYLTLITQLENQLRLKRLTLQRMWFYLQPTYDTLELLYKLCLESSKQNYHGTQVLNLLIKFSQEHAIDKKSQDLFQYLLQHSIQPLLEMLESWIHKGVIRDPFSEFMIEENTDLKRDNINRDFNDIYWDQRYRLRAEQVPVFMECVSSKILNTGKYLNVMRECTASIELKKFDPIHYNANELVYLERIEEAYDYASGILLDLLIREKNLIPRLKAIKYYFLLCKGDFFSHFMDITYDELKKRLSDINMVKMNSLLQLSLRTTSISEDDTFKDDLECEFIPYKLSDQLLNIININQYDNNRTRSATTTSSSSSSGGQTSDSSTSTTFSGKSTTTSVSITSNSVHDSLKTKELLGIESLAFNYHVGWPVSLIISRKSLIKYQIIFRHLFLCKHIEKLLNETWVDQQHSRKKQYSNRPGVTTLLAFSHLLRHRMIHFLQNLEYYMMLEVLEPNWAKMIEAIKKSKTVDDVIGIHNNFLETCLTECMLTDTKLVNILMKFMSLCIIFTNFTNQVTQESNTIEPDKVKRTIELFEIKFHTILKLLLDTLKSFSTIESNRHMVHLIHRLDYNNYYSNYFEKNPNLFEPRNQNNNQNNNNSNGQPDQNSTFSPDLNQSFVSGLNINNNSNQNNIRNSTTTTTTTNTAKSKTPVKISPPLNSFKNDYSNNNSSSNQFSSRNSSANIAPTQSGNSGAINNSNNSSNSFDDIKKRAAEILRQQNQTSSNSSTQTTNNLSPSFANLHINNTNGGDFSQSVQGFQHRLKNLIGNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UTT5</accession>
    <name>A0A8J4UTT5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_010473</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001135">
        <property type="protein sequence ID" value="KAF2068203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UTT5"/>
    <dbReference type="OrthoDB" id="269151at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01856">
        <property type="entry name" value="YlqF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45782">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45782:SF4">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="4">
        <location>
            <begin position="157"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="329"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="332"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="356" mass="40179" checksum="EB2099367DD045FC" modified="2022-05-25" version="1">MNQFRSSFQYNGRINWFPGHMIKTRKLLKDTLKFIDVVIEIRDSRAPLSTENPILNELLMTNNNNRKSRVIVFNKSDLSNLQLQKRITDYFEDTAAQDKRKGAVAPMIAFTQSHANPSSSHPRHPLQLLKKAAEMHLKRYPPPTSYIQSPLQKEINMLVVGLPNVGKSSFINSIRSSSHKSKAAKVGALPGVTRNIRGFRACDSPPAYLVDTPGIMIPGNLEDTEKALTLSLLGSISEKIVPLHVVADFLLYKLNQLNNTKYLTAFELDPKKPTDDINELLNHICIKKKMLLTNNEPDLDQAAKYFITQYRDAKFGCFTLDTIPTIDKQLKNDNNNNNNNSNKNTSDGNDSDNQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UU25</accession>
    <name>A0A8J4UU25_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ATPase AAA-type core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_003710</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000120">
        <property type="protein sequence ID" value="KAF2074971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="29773at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389:SF13">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="280"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="309"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase AAA-type core" evidence="4">
        <location>
            <begin position="946"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="386"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="430"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="606"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="824"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="386"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="453"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="488"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="538"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2074971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1441" mass="163137" checksum="A721FEAD94B5A847" modified="2022-05-25" version="1">MTIKQQLQQPTSGTSKFTDKFASVGMFFVLLFLISGVSNTLIVQLIYNNITFSPKTMFTNIFIFSGYAMLNFFKDTSISSSSSNNSNIKKKPSSKISFKKYLLLALFDCAASLLTTVGQIAVGSGLFQVLFSSKIIFSAILSRFYLKANWTTAKLISILIIFGGLCVCVYRPSSINSNTTLLKTDNSTTITTETTSTTSAQSQAQFIFGCGAVLLASFIFSASHIYTESIMSEYDISPFQFAAKYGTYSVLVCFIYIMTVTVYNINEWVMIPINRATHEQAIFVFVLFCILTITSVCRSSSIFTILAQYGSVSMGVLYALQSVIVFFYSAFFLCDPNHPLKSNQCISYQKVLGSIIYDYVLLLSTIMIDEYDSSTIITTTAETIDSNDNNENNQQQSIETINKDENLTPNTGTKRKTIIIRKLLSFNDSDDDESLQTPITIRPISTRRKKQKTTTTTTTTTTITPTPTPTASIKDNKQPNDKILSNDSQSSITTTSSSSSLSLSSTSHLYSPPLSPVSSSSETTADEKSLNKKKKTTTTKTSTSKRTIKSKSTSTPHNNNNSLIGFLVNNNNVQTTATTTTNIIHTTPAIPINNNNSDKGVHPFFKQEEEKRKKKKEQEINQQKLEEKLKNGSNAVADMFLSKKERDEKNLLKQQELLRLDIMQSIEDHKKVKNSYEQAGGTTKQLHPFFAQIQQQQSNNKQNISLHQGTDQFSPINVDDAITTTSTSISISTNQQQQQTPAKLYNLLEFPIGESHFGLDPIEVSTKILPPLLQIDSSILNRSRLIQDDSCLEFDLNSCIPNYSNILSKIINNLNNNNNSNDIIINLEDDNNNNNNNNNNNNNNNSEIKNNIINLIQELAEFKEKDNIFNYNNLKSIIDIYYNSNNNNSNNIYNYNDNNEIININNQIEQCLNQWINNWHEKLVLKKNDGNSKRKNSTPLTICNCILIVGPIGCGKTSLIFSKSKQHHFRILETNPSSKRSGKAIIEMLGEATQSKSLNLNSADNNLVLFEEIDILFQDDKGFLNSLNTLIESTKTPIILTCNELNSSISNLVHENDQIMTLHMNQSDTKSLAIYLCLILLSDNDVGAVDKYKFLLQGQFAFNNICQFIQFNNNDIRKCLLNLDFILSNNNSSNSDNSRSNNEQYVTDLVYYQFSNIINSNNGLSNLLFSFNENDDDHVDHLALILNFNSNINLYFNNYLNYIVKFQEKDHKGIKSIVNNTTTTGDHNDNDNCLLSSNQSNNINIDDIWKVAEDFSFNDIISNRNYPFNQYIIEGDEELYELVENSPTFIMVPESLHYKDGNNFKSNFKSNLDDDISTSISIYSIKNMCNNLSLLNNQQQQQQQLTKWNLNYLEIQSQYKIIETNVDQDYIYRDIYDSLIFKNNADISSTKLKIEYIPFLNIICTDELSRKINNPKRGNRFNHYLDYTDDQVNTLFKFKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVQ1</accession>
    <name>A0A8J4UVQ1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001912</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000049">
        <property type="protein sequence ID" value="KAF2076786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVQ1"/>
    <dbReference type="OrthoDB" id="10250320at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20540">
        <property type="entry name" value="CYCLIN_CCNY_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000302">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012399">
        <property type="entry name" value="Cyclin_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14248">
        <property type="entry name" value="CYCLIN Y, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028934">
        <property type="entry name" value="Cyclin_CG14939"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0195">Cyclin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="3">
        <location>
            <begin position="219"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2076786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="383" mass="43685" checksum="F8773581D2ADA1E8" modified="2022-05-25" version="1">MGNCCSSKRANGYDPEQIDDAQKILDELTISSNTATANNNFATSKKHVLSQHHDLKPTITENKRNSIKTKIIIDEREDLISATISDQKDPFEVPDHNLYPKVVAHSYPNTHKRQLSTEKVGNFGPQFKTPISSIPHHMRMEGFQRIKHNSTSSLYIKSSLSTPDNDEILRCMANALLYHIERGSQPPQKTIDIFSEEKYPITKGKLDLKTIPTVDTIYKFVRDIFKAEKLDSECAIMCLAYIERIIAFTGVTLNPTSWRRIVLSALILASKVWEDQSVWNVDFLPVFDNLTAADLNGLERQFLALLQYNVSLTASVYAKYYFELRTFSKLDSNHFPLKPLDKLGVRRLEDHSKASEYRVKPFKRSASVDQINPPEIHTRAVLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWN7</accession>
    <name>A0A8J4UWN7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008786</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000577">
        <property type="protein sequence ID" value="KAF2069900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWN7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550:SF10">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="633" mass="74232" checksum="057D9C8AB59ED14D" modified="2022-05-25" version="1">MTTYFNLVFRNKYIVTEIFKKVHEIQQNDNSLHYEEIKDVIWMIKYNHYGLLREKIRNNELLYLNNKSPNYIVTFTSIKYTDLFIILYERFKPILLSMDYFINQCKAANRVDLITVLGERGHLLLHGPSPTFVPENPDILKVLVANKNFNFGEIDTFRMGHTAESLAIVMQNVKLPLTQSDADKLWVLLIKSGRPNVFKEVAQLLKPFKCSFPFRKTNYVQIKDMDLFIHIWETSTHLDIHQKRSWVMEKIITDPNSVSLLSYLRNRKDPVIEYMEDCIDFVIENCIKEQSKVTLNYILDEWLPNHTLEELDLGLDMYNRSFESGFYSKRFVEKLLDRGLDLFKYWISCCVDHGAEDIFYMLWERYSDRIRVRGSKIYLKEKKFTEQDITSIILERCFFISNIQVMAFLFDKGLNLDHGTYSLAFSHLGLQNPKVSMEFIQFLEKSYPSIGPISCVHLVKSLFNLNQKFDKLFEYGIDMIIRNGDKTPENESDFLGKCILTRNLYRLKYLHSKGFVLRQEGELNLESLLAATSISILEFLVNRYDFIKSLLVKVSFISNVVLVNNYSLFKYSLSKGDGSFVFCPNIEVQIAQSNCVPLVDFIIKHIRIDSGTIQQLAKTNNNTLLLEYFQNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXQ6</accession>
    <name>A0A8J4UXQ6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_007432</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000396">
        <property type="protein sequence ID" value="KAF2071250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXQ6"/>
    <dbReference type="OrthoDB" id="1405469at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004694">
        <property type="term" value="F:eukaryotic translation initiation factor 2alpha kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13996">
        <property type="entry name" value="STKc_EIF2AK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF193">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DDB_G0268642-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="394"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="66"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="483"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="692"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="878"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="910"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="483"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="495"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="515"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="545"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="695"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="878"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="424"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2071250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="939" mass="106151" checksum="EF51825D179DF726" modified="2022-05-25" version="1">MENKVHLRSPYAHILDSNESDESIFETKKRTIINSNNSSGRYNTRYDLTHTSYSLPLTSSNTADDISIGDSSSSSNNSNSNSNSSNNGDDDIGEQDDSNSNWSNQSKEESDRLISEYKKLFKTQPTPSSSPISSNNNHLTNIRKSLTNIIFEEKSNESDGDDDDNDDQNSIYEDYGQESCKILDHNIDEAFGNNKKYGLQVTRKDIHYYDPLSSSKSNSSSTEDIIKKKPSSQVLSKDIEGVLQLIESCDDMFKQKKILLKMLLSEHFIESNRNEGQIINKKKLFSFMKNIVSVELSDISPTILKSNQFKTLYLDYYRKIFQHALDLTNIKYNNNNNNNDVVNNLNNNKLSLLTNNNNNNIQVPQEYFNPVSYFINQGPSKLINQDSSRYEREFEEIKKLGSGGFGSVYLTRNLLDHQLYAIKKVNFSISLSSNNAASLKIEKVLREVKALAKFDHLNILRYFNAWIEPESIAHNVYSSAEGSMNTGDQFSTLNTEDDDDDDDDDDDDDEDETQLNSTTNYDSTTNTLNNNHDSGSDSDSSNGDNDNDNDSDGDDSESDDEFSYSEGNSITLSTSKVLIPYRPPAKNKAIKSSSPPKSLVTVSPSTKIKYCIYIQTQFCESGSLRDWLDRDDFKNQSMKSKSLIFKQIIKGIHYIHGMGMIHRDIKPANIFLTHDNIVKIGDFGLVKDLGMNNNSSPSSSPSSSPTSSPTSSQQFPISPPTSNLHKSINIYKDPHSHLHNHLHHHSYHFHNHRNELNHSHTNFVGTATYSSPEQQTGGSVSYTNKVDVFSCGIILFELLYGPFSTQFERSDQIKNLKNSVLPHWFIEKYPKESELILLLVAKNPDQRPSTGDIISKHFPLILKEPIIIDDNITTSSNNNDNSVANNASNTNNDDQPNYQRLEHSNLVELLKEKDIYISKLLKEIQDLKQNNNNNKTDPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXQ9</accession>
    <name>A0A8J4UXQ9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">IlvB-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_007423</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the TPP enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000395">
        <property type="protein sequence ID" value="KAF2071265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXQ9"/>
    <dbReference type="OrthoDB" id="16262at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02004">
        <property type="entry name" value="TPP_BZL_OCoD_HPCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000399">
        <property type="entry name" value="TPP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968:SF166">
        <property type="entry name" value="2-HYDROXYACYL-COA LYASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="THIAMINE PYROPHOSPHATE ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00187">
        <property type="entry name" value="TPP_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="9">
        <location>
            <begin position="57"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="7">
        <location>
            <begin position="280"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="8">
        <location>
            <begin position="475"/>
            <end position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2071265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="639" mass="70108" checksum="6A8AC94B77780C41" modified="2022-05-25" version="1">MLSTLNTAIKKEPSILMGLVSGAAIAYAGYKFGCYVGENDLFTKLPSIDYPFTTESNGGELVAKVLNEQGVKFVFTLIGGHISPILIGCEKENIKVIDVRHESTTVFAADGVYRLTGVPGVAAVTAGPGLTNTITALKNAQLAQSAVILIGGATSDLLKGRGSLQDIDQFALVKPHVKWCSHISKVSDIVPTLEKAFKIAVEGTPGPVFIEFPIDTLYPQPITSSWYQKNKFQNQTLYTRIANAYTSYHLNRIFSALNTTIHIHTKRSLLHKTPSQSHVNTLIKKLKQAKRPVLVVGSQVTQLNDLYSIETLQRAIQNLEIPVFTSSMSRGLLGSSHPNLFRHCRSYALKNADLVILAGVTCDFRLGYGKSINSKAIVFGINKDSVDLYKNRSPTYAYDYDPGTFLVNLYSNIQSSKLPENWKEWADDLTGKDIQRNIEISNLARTPSKQGFMNPLRALQMFDESLPHKTIMVADGGDFVGTAAYIVRPRGPLSWLDPGVFGTLGCGAGFALAAKLVHPDHQVWCIFGDGAFGYSIPEYDTFVRHKLAIGAIIGNDACWMQIMRDQKDFLGSEVACNLAYTDYEQVVKAFGGEGFKATNETELKSALDSSELILQENVKPVVINCIIDRTDFRKGSISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYC8</accession>
    <name>A0A8J4UYC8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Spermatogenesis-associated protein 20-like TRX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006948</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000343">
        <property type="protein sequence ID" value="KAF2071743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYC8"/>
    <dbReference type="OrthoDB" id="1923667at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02955">
        <property type="entry name" value="SSP411"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024705">
        <property type="entry name" value="Ssp411"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004879">
        <property type="entry name" value="Ssp411-like_TRX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42899">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42899:SF1">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03190">
        <property type="entry name" value="Thioredox_DsbH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006402">
        <property type="entry name" value="UCP006402_thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spermatogenesis-associated protein 20-like TRX" evidence="2">
        <location>
            <begin position="48"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="766" mass="88026" checksum="9F3C8BBF628619CE" modified="2022-05-25" version="1">MSDKTTTTHTTSTSTTGTDNNNQKESTNTTTAKNIDNKEQPQQQHEYTNKLIHEKSPYLLQHAHNPVDWYPWGQEAFEKAKQEDKCIFLSIGYYSCHWCHVMAHECFEDPEIAKVMNELFINVKIDREERPDIDKIYMTYITEVTGSGGWPLSVWLTPDLEPITGGTYFAPTSKYGRPGFPDLCKKLESMWKNKREMVLERSNTFIEFLKEKRPAGNATSAISEKTIDLCYHKIMEGYDSELGGFTDAPKFPRTGIFNFLHQVHNQRPEGLKALHFTLTKMAFGGMYDQLGGGFHRYSVTPDWKVPHFEKMLYDQGQIASVYLDAYQITKNPLFAEIARGILDYVQRDLTHPDGGFFCAEDADSLDVESGHNGEGTFYVWLSSEIEEAINDQEKYNIFSFMYGILDNGNVDPSDDPHNEFIDKNIIMKIKTSQECANYFKMTVDEIDQHIAICKQRLFAAREKRPRPPLDDKIITSWNALMISAYAKAYQIFGEKSYLDSAVKAVDFIKKNLYMADKGLLIRNYRHGPSSIQGFCDDYSFLIQGLLDLYEASFNTDYLEWAVKLQEMQDTLFYDTQDKGYFSTSGQDKTILSRLKEDHDGAEPSCQSVSSSNLLRLYAITEDQDLYERKAKETFEASALYLDKAPIVLPQMVCSLNMYLKSMTKFIIATPSFENDDFKNLVTEIHSNYIPLKVLIVRVGTNGDDHAKNDNADRFFKEKTKNFTYEIAKPIYDKPTMYMCNSLGCQPPTYQIDDIKQRLEKLSKNIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYQ4</accession>
    <name>A0A8J4UYQ4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005293</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000207">
        <property type="protein sequence ID" value="KAF2073380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYQ4"/>
    <dbReference type="OrthoDB" id="18475at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="234"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="283" mass="32397" checksum="3C79760C1E847064" modified="2022-05-25" version="1">MNEKFQYPLTSPFRVIVRSKRRGFLKRHFNDDMPIELNPYVPQENYANSIKYLNQVAFYTILGQLVSILPVLVCGIVMFAVGEARRQSTNYTPIYWYIGILILGVSFLCLLVAIFTLRSRYIRNLSYAVQKIHTEYNNSFGIQWVMKYVEFENRKGKRRIKLWCELIIPNSPIAQQPLVAHPQAYPQHQQNQAQYVPMAYYYGNQKPGEPLPPPTAGPSQPFSGYGQQLGGGPSSSSQIPQPNGSAPYYSYPQNNYVNIPIDQEYPPTSSKPNVNLDKESDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZD7</accession>
    <name>A0A8J4UZD7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005830</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000243">
        <property type="protein sequence ID" value="KAF2072853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZD7"/>
    <dbReference type="OrthoDB" id="20889at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47824">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47824:SF2">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="410"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="421"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="667" mass="75253" checksum="1ACC3E2616F6A70D" modified="2022-05-25" version="1">MIILIKTPTVTFSLDVNVADKVEVLVKKICERTSYSEFQFQLSFAGETLDYSSEISSYGIKKENTIHLQLIKELTFDVEYKNAIINVTVPNTADLKIVNLKSHICSVLKTNEPGIYSLPLVLSQGGKQFTDNELVHKVVKRMETLVFEQKATSGANDSTSPDTQDLVPMINNEDILAHFAENSISNDVEIVFCFDTTTSMNAVIVEVRKKLLETCNRLMSEIPSIKIGIMAMGDFCEERPLYILDLTNDVKKIVSFINGVKSTSGGDEPECYEFALHKAKFLSWSSYTTKAFVMIGDSPPHPPYYTPHHLNWVREADDLEALGVKIYGIVAIHEKYRYFYQTISERTGGLCILFSRFDLITEMFLAICFREASSAKYEQYKKEVKERGGEGLDDIFKDLDKPNFELNDTAAAAPSEDSTIPETTTTTTTTEETTTTEEKKPEVVEKPKEPTKIAKKYGYINNYPDKSEEWCNFLDDDVKKNHSFALGLFGGKYKAVKKASSYTPPIIGMVIGSRHIGFHSFMKTLILKTLKIETRLYFNDYRLDTLKSIPFGSLFIATFSVASKQSFEEATSKIEFVQKEHPTVQVYLLGLQSDLRKTVKPVTPDTTSNEKESETAAAAEVTFVEKSDIDLLIRNKNLLGYSECSIHDPSSITSLEKKILKLYEQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0L2</accession>
    <name>A0A8J4V0L2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome recycling factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_009302</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000675">
        <property type="protein sequence ID" value="KAF2069377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0L2"/>
    <dbReference type="OrthoDB" id="407355at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.40:FF:000001">
        <property type="entry name" value="Ribosome-recycling factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.20">
        <property type="entry name" value="Ribosome-recycling factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002661">
        <property type="entry name" value="Ribosome_recyc_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023584">
        <property type="entry name" value="Ribosome_recyc_fac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036191">
        <property type="entry name" value="RRF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20982:SF3">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME RECYCLING FACTOR PSEUDO 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20982">
        <property type="entry name" value="RIBOSOME RECYCLING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01765">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55194">
        <property type="entry name" value="Ribosome recycling factor, RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosome recycling factor" evidence="4">
        <location>
            <begin position="102"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2069377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="260" mass="29287" checksum="4CE8A3CD05C7D7B4" modified="2022-05-25" version="1">MMMIRCINKLNKINNFVIGNTVKRSYGTSTTLISRRSLTSLCSLGEQSSSALSMTSTLQRAAKKGGKGDKKGAEETPYDKLSAVDLNPMKEQCERTMEFLFREFGNIRFGRAGPELLERINVETPSGPMQLSHISMITIKDPLTLHITLYDTELVKAVEKAIQTSNLEITPIVQGSLIRVSLPKPTQELRAKLVKQVNSIAEEAKVSIRRHRKDGNDLMKRYKLVKDDERTLEKNIQKVTDDYVAKITKSTDSKLKEINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0U8</accession>
    <name>A0A8J4V0U8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008987</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000613">
        <property type="protein sequence ID" value="KAF2069692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0U8"/>
    <dbReference type="OrthoDB" id="6130531at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7743" evidence="3">
        <location>
            <begin position="395"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="2">
        <location>
            <begin position="625"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="1">
        <location>
            <begin position="764"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2069692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1059" mass="118350" checksum="564065CA81828AF4" modified="2022-05-25" version="1" fragment="single">MELYALQSNQQSPYFSNATTPCLCTYYIQVVNPPNSSQAPSSCSFSTSLGCTVSSQFNDTNQLLRIELNNKLDTSGEVSIALTYLDGSSQPETKVYLKEQPTVPLRYSCSSFPSSEILNIGSTNNKIISRDTSYSIGGNMYGYIKLKTKGSSLIVPPGYFSTNQDVLEQWIYNENYWYRVQTKTLNLNTVELLKESSFAFDLTVGSQFSFYFPNPYSQREYSQKIDVFDLQVKNSGIGNVYSVLDLSLEKGIIPIMNQAMIPMEGKNTYFSAFFYQGVFSIGIYNGSFSIVLNSGVTNEVGDVLNLPTIKVKSSLEDYYVSNIEFQNAVPFSFTYITSTISVPYIYPFGLVGMNISTEGQIYRIPQLRDPLNSYFFIINTYPVATVSESSLVSLADNVIPIIKSVSIIQMPLVGKNQVKNILRINALDELSGVSHIAVTFSFETIYIDASDLVGGNKNDAIYEKDIGRYHRDSFFSLNVFDFNLNSHFYTPSELCFMFGVGLPLSHMTFSFEDIKEFYFQYPEMTKSGGKNTAYLSLKQNMDSLYLTIYSFKIVFMFNLQNTQVSYGVFNPASNQYEFDINVPANLMFGNIKYEIYANDAKTEISNESILGKNNTVKLAIVSQEFDQFFPTIYSLVPQTTVFQGDSFTVSWLVEIKDYSGLKDANFTMIGDYDQLGYTLNIKPQDMIGGNTQKSEYRVSYTIKEKCRNMKYYFSVIETMDQLGNYGKYVRKENITLSPYFRFDWKFNDAIEVVCPTQDTDLTIPELKNVKVLTPSLNTRSSNRIGTAVFSVEDLGSGVSQRHIPSCYFHTTPYKHLGVQAVITAQSNSLISYSCSIEFPYGWGADSFIILSIYGISDNNYNYVGYSAADLESKFFIDARSSNMDQPIISDISGYIQSDTRMIVYGYNFISTGMKIIVKNTTSTFETTPTFISNTAILMDASLMTTDFTIQVSKPSLGVVSNVFNVACVRCIKEDSSDQVTIPPVTPTCRSDCGVPQGYGKCVNGACVCNPPHSGIDCKAKTDNTTVITPDPVKPSVNLTIPGTNSGQTPEFISFVSVVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V175</accession>
    <name>A0A8J4V175_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_003456</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000108">
        <property type="protein sequence ID" value="KAF2075238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V175"/>
    <dbReference type="OrthoDB" id="20669at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22117">
        <property type="entry name" value="F-box_FBXL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19855:SF11">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN WDR12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19855">
        <property type="entry name" value="WD40 REPEAT PROTEIN 12, 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="5">
        <location>
            <begin position="707"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="850"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1025"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="515"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2075238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1147" mass="132363" checksum="6E4D7F1D4B50B0E7" modified="2022-05-25" version="1">MQYVNGYEIVKTISDEVRFIFTNPNLVWTEVAEYEGCKAFYLNNPRDDGLLFKCVADIDCSPEIPFNVVFHTELQSEWDDLCIKAKRLDVLDSFCSVDYFQFANLIGGYPKGFTLLRNFSIDYDMKACFVACHSVEYDLSSQEFHQPSTILNSLRPSGWYMQEIAPNKTRLTVMSQLPDIVMTGTTAKIDPEQKKKIYQNTSIKGLYIVPNLIKYIKEKYLLNNQPSPTISIKSSPPPTTAFQNSHRKLSLKYILDHIMTLPDHDRFLKNKDISEIKDQYIGPMDIFKSASEKIGEFENANWSLFRKINDFCFYYRDFDHESVDYVMSGSFHMKVDINTLLEYIITEESHVVDSWMYGAETVAQFDDISSVVNIRYHSVLDPANSVSSLILKQVNNIPIGGKFIGFRSLVEPDMPRDNFWFYPSGYYISPSETGGVFLRYFLSFRAYLPTELKKVTSKQNILLCISEKGLYTMTQLKNIQYKSATGGYIDMNPNFPHWTNLKDRPLKEVVDFFATMNVEDQQQSYERKKQKIQQEEEVEKLKIKQAAAELRRNETEIGVSYPDIKGVKPFTNLIKLAKEDKKRKLEDHGDIALSFPTSKFTFKNPDEVVLENSNSSNELNSNSNEEILLSPTTSIHSSISSGSLNSLDEKLASISALEKDFIYFKFTKDWDKDLVRKPFFLLMASEKSNYNPPRKSPRSNQYPNLTNSLFDFLPDEILFYILSFLDTKSLSRLSLTCKFFLTYTNNCELLWKELFNRNYKSSTQSLFRIDLMSSLNHMNTMAATNSENRTFNICKTVDRDKSKEEIKFLDQVDEAYYNYNTLNNNWRNVCVQKEIIKNNWKNSKPKISLMRGHKSAITCIQQSEENLFSGSRDRDFKIWNLSTKHSKITKDQSNASVVSFEKDCFTKLADELFPYCLYDPSMIRIGYSNGYIHNYNMYTKKSIEQRFVYVADGFLFKNNNALIWEGTNLQMWDQETSSREFECNLDTKINRCKLSGTMPTCLFLACSDKTLSVFDLKTNQIIRTFNGHTASVNCLDFMSEYQIVTGSSDKTIRIWDIRVESELYTLKSHKSKIKCLGICGSTIASGDSNSILVWDINNSNNSSGNIDQHIPRSTFKVNSSVECLTIDNETMVAGLFDGNISYFDFFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V177</accession>
    <name>A0A8J4V177_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MRH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008478</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000526">
        <property type="protein sequence ID" value="KAF2070202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="23204at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23524:SF1">
        <property type="entry name" value="MRH DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23524">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G04850)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035304831" description="MRH domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1254"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1211"/>
            <end position="1248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1213"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1232"/>
            <end position="1248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1299" mass="142960" checksum="E60A44774D0CBDA6" modified="2022-05-25" version="1">MKISLYIFPFLILFVYQVCGQACPTLTYTVQKKCPGNSSPYVVDTFVQIPTSQPQGATYSFSQELQPQIATYAVNKGPVYFLPHGGEYTLSWSFSNRPECANSTTIVPIITSFVVNHPKCRFSQGNFNFINPSSDFTYTQYPSYLYTQVAGLGCERNVVYYELSDAYPNLKIENTQCHKSTGSVQVLNDGAYSVMKLLLPDGAPIPTPSPGLWTQLSHHQQYTLALESDQCGDQFIPIDVFPNIPKFELVFHKNESCPTNSSVSVAFEDPTVFDLLDSITINGVAYTGEPYYFDFTSDINLALSLNGCSYARQMGKPYLIPDVHYTIEQCKNYNSTVNLIYDENVITDLQVSAGTNNSFSPVNVANKQFELNENQDQFYYSSQCYSSAIVQISSPVLPIVVIENPPQQAGDLFDIFVSNYMDFFELYLENTSLAYTKADQGWMRNIVATDTYNLKYRVDGCARYSLPISYFSGYLDSSFQDIITVISPGTCNRRGLVSFKSYYGIYPSEETISLFAPGELHTLTTTFPQQFPWGSSRSKLWTAPPDPWSFEQPNFNITVSKNCSCKYSNDGEIKITYMSDFVILAIEIDGIGYYPAGDGYSYIFSGLPCGELRVEVFEDLCSYRYYMIQMYSNSDFEIEYTTFPADPGEYNGGLKFNASQFSTLVGPNGVLLQYSGEFFNQQQNIYKFIFVTADLSCSGSFYALIPTTVPATVEVIPYTRPFCKKSLSGAFGVSLFDDNSVPIPISSIVNMKNGKIVNIATPPVVPAREYNYAIRSPSVVRLLNYDLIEQDIEFSYNILSEMGSPNSCQVWVFVELIPSVDNFVVDGVFANHYPLSKATNLFKYESRGESSFTVEIYYGTGCSKSLVIERNLQDKYILKNDWPNLSVTQNNCSDPDSINNIVEVHKGSGRVQSSLSTMSADLNGKFVQTSLGGSSADEITFRDPYSFCTITKTIPQTYSTSTVTVKKGICPTSNDGTIDLPIDTNTQQALVKEVISEQTSTLPVASSTSLNVFTGVRNSTFTILKTFKTNSFCSILESVQVTVDEPRVESVASVGACHIDEISLVNGGQIINTLSINTSSVTYSAGGISSIDDPVIKGLASGEYSSTVTINNKICKRVLLSNTVTVAQLPTVSTTIDVSQCAKATITPNQMQIQHKYIIKDSSNQTVNERNGIIGSFSVDFTKAGTYSLESSDMADTCSFKASFQVTACPINPTTTPHPSSSHESSQSHSPSHSESKSDNPENIQSKDDGGVNLGLAIGLPIGLVGGAAAAAAAGFFLYKKKFSASNKSVGTKMRGLPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1G2</accession>
    <name>A0A8J4V1G2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carbohydrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003229</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000099">
        <property type="protein sequence ID" value="KAF2075448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1G2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035306263" description="Carbohydrate binding domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="3">
        <location>
            <begin position="440"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="2">
        <location>
            <begin position="660"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="852" mass="96167" checksum="3552610FB209BF1B" modified="2022-05-25" version="1" fragment="single">MKFKYLLSLFLFISLVASIQGVILKNYAKYADSTTSNGICTNYLYLVFENAEVIEAGGVHLRIGDNEPDFIIKKITTSVDSSIQTFTLSFMVLDYFLNTNSALGPFSLEVIDQDQQSSFFPLTHYYPECTLPTPFQILSFYPVKYDFSSQMIKIEFAVVNDIHMEKPNSICSSTWGPCQVENLKTFLYVIKIQPKLSIFSNPSIDVELSAGVNELPFSFNSPFNFNNLQGMYQSLALISPNPGSFSETFYPSIKGVYVKYIAKKTEPFILYTYNLINDTAYSFAPLLSDGLSTTMITLATMREPNLGNNQIQLSVVGKDSANLPSVQTVQTLLFGYQGFNLISFTGTTAFNRVQSVLGLFQLSLDATNTHNEPKWTNFMGDVVQMPYPYGFTKTSSAQIPSYKLEQLFPALKKSYSTKISDYFHRESTFSGVDITISEVTTVPFIESQTISRFNSYISVLTIKARGPLPGIYRFISHYPSKVFLAADVLASGTVYDGTFDLVFDATEFIDYDQNLFYVEVESFAKTKLSSKDFPGLDLEQPKLFENPIILFDSFSFLHKSLDKNNVGKKNIVLFSINIIDIPILRIKTNNYFEPAKYESYIGSYSSQLNMYVIEFELPSQSLPGELDYSIFIAGNEFTSAEMQRLFADRAKLTVDTTTYDFMPPMITSFTQFVDSENDPNQVEFGWYFQIKDQLTGFDHGVIHITANHDTEPRIFNITEKDRAWGDQYDGSYKVTFKVPREQCRELLYYISYASLSDKNGITSKNFDPYALNPVTELMNNHAQVHICTTPYPNVQLATLTAFTLSTSNTGMTTASITIRDIGGLGLSTRHHPYIFVQGIVNYIKIETVLSGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1I9</accession>
    <name>A0A8J4V1I9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003200</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000097">
        <property type="protein sequence ID" value="KAF2075508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1I9"/>
    <dbReference type="OrthoDB" id="1716625at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051092">
        <property type="entry name" value="FYVE_RhoGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673">
        <property type="entry name" value="FACIOGENITAL DYSPLASIA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673:SF159">
        <property type="entry name" value="LD03170P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="2">
        <location>
            <begin position="409"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="383"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="385"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="841" mass="94175" checksum="9193EDE685580AF6" modified="2022-05-25" version="1">MNDNSNSNVPSKRNSMHIRSSSEYNLRSIGNGGHSGNDGANSTNIASPHGQLSRSFVVNSPTHKRVTSIGSKTVSSPIGNPKRLSGELEKSKQPIKSFIADRVKFFSDKEAIQKEKQIMSPGTPPKGVVLNHISNSKSPSQLDLQQQEPSPSVNNSHPKTLISERIKSFSNLNLNDHFQNNINHHQQQPQQPQQPQSKPKAPVRINSMLNISQIKNNHQYQPPAPIRQPTKNVTPRELPLPPTPENKTPRENKTTPKENKTTPREIKTTPRESIPIISLPPPPTIIEPTLPPEPLQQPKINNIIESSTSIYRSPPPPPKNYMDKINISKNDIQISLENPSPVLRGSSVLSQDQTLVDLNFDEASSIADTESVMVAIQNLSKYEQSSSSSPPSSSPSSSPNPNTMAKLEQQCKVLTELYSTEQDYLRDLDFIINEMIKTLSAKGFDKTIVMGVFSNIEIIRNLSKTIVDDLKSCKDGITLHNVVTVFKKMSAFFKMYSQYCTHHSKSMDMVVELSKNNEKFNNFKTEIMNLPESRGLKIQDYLIKPIQRICKYPLLFNELMKTTSNEDPDFKDLEAVHSKLVEVAAFVNEFHHITEGNERLLELQNIIEGAPFNIIESTRKILKEATVKMKEFEKDDYHTRFIFIFNDTIVFTKYSSFTKKYHFSFNLPYHSSRVTPSIKDRSFHLSGTNKEGVAINLELQCEDEQEKDTISNLLIDLMATGKKHFETMRVRGNNKLSPTCITKGTATLSVGGGRDALSRKSSIHDGLAGSSTLPISGSNSINKYSSSIADPLSPTYLFNKSSTLSTTSNEANQALFNLSKLMKDSGQDIKKKRETLNITKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V296</accession>
    <name>A0A8J4V296_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_007374</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000389">
        <property type="protein sequence ID" value="KAF2071312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V296"/>
    <dbReference type="OrthoDB" id="67700at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035329">
        <property type="term" value="P:hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051583">
        <property type="entry name" value="YAP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17616:SF8">
        <property type="entry name" value="TRANSCRIPTIONAL COACTIVATOR YORKIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17616">
        <property type="entry name" value="YES-ASSOCIATED PROTEIN YAP1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="6">
        <location>
            <begin position="307"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="6">
        <location>
            <begin position="443"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="344"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="358"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2071312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="501" mass="56654" checksum="84D6CCFE4D4A0824" modified="2022-05-25" version="1">MDIFNISIHSLSISHSRKKRSLYVVGDFDAYKQFKTELKKSSGEGECGWGEFKLNFEYQTKFIHKLDHKHFKLYLYKKKSLAADSLVGQFQVDLYTLATGPISHDVIFTKGNLGVGRFQFKLEMSHVNKVRISIPSLYISNLISKSSNDLVHHMEYSLNKNNVLKMPQIEGSTCPSWFNQVPIEQQLSLKDLVDSNICFTLKEQRSRDNNSGELLLPVKSIFSFIEGDMKCVKSFLVNVKGEKVCDITVKVQFNNIPQLSQMRGGIQTESGIRNAEPFFSGVPLPKLVGEISSEQQVQNPNQQQQAIKLPAGWEARYDNFGRVFYIDHNTKQTTWISPLVSQPVENKKRAEVSASRLPGSNGSGNESGPSTPVKSRPAPSKQDKDEAAKLIQKTFRKHKKEHYNKTVRNPKEFVTPVQQHTSIQQMYPSIYNSPSRQTFDINDPLPNGWEIRMDRFNRVYYVDHVNKTTTWSSPNIKPRAHSVMVGDGGGGSGGGGYAGYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2C0</accession>
    <name>A0A8J4V2C0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Coiled-coil domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_002545</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC124 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000071">
        <property type="protein sequence ID" value="KAF2076138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2C0"/>
    <dbReference type="OrthoDB" id="76412at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010422">
        <property type="entry name" value="Ccdc124/Oxs1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054414">
        <property type="entry name" value="Ccdc124/Oxs1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21680">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21680:SF0">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06244">
        <property type="entry name" value="Ccdc124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Coiled-coil" evidence="4">
        <location>
            <begin position="167"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="11"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="128"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2076138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="255" mass="28996" checksum="1CD26A4032AF4C8D" modified="2022-05-25" version="1">MGGKKFGANSKAEEAKAKKQEVKKTENERKTREKEDALWAENDPKILAKEKKAKEAAEKKEAEAKKKAEAKALLEKEDKEIKSKYAKPQDKVTRFEIQKQKEKEEKEREKLEATQDKKNSRVVVNHISNSSGSVPTTTTTTTTTTTTSSNSNDNDSDEDESFGLQRNMNHVLREQREAEGEDTIDARDINEALNALSVGGQDKHPEKRMKAAYAAYEEENLPILRKENPSLKLTQVKQVLWKQWLKAPENPMNQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2E7</accession>
    <name>A0A8J4V2E7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_007239</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000374">
        <property type="protein sequence ID" value="KAF2071447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2E7"/>
    <dbReference type="OrthoDB" id="423607at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050648">
        <property type="entry name" value="F-box_LRR-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13382">
        <property type="entry name" value="MITOCHONDRIAL ATP SYNTHASE COUPLING FACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="40"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="221"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="584"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="584"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2071447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="603" mass="66852" checksum="BBFB9700B7818179" modified="2022-05-25" version="1">MKTISTLVFDNKSGGDSLGKRLSTSTTSPLATLFNHNGVCMNILLLPLELLEKIFKLLNQKTLYNTCLVSKQWNKAVYMVWHTIDLSKSHNHFTHLTSRIKTSISNIKSQKIKYLEAICNNLISNNGFLIPPLITSKKDGSSQQLSIFLKNYNSSSSSGNSSNSSNSINSNSNSSILYSSNSESDKQQQLYIKQQQDVSLNNSSLENNNLISDQTNNSIENTDLISNNSNLNNNSNSSNDSNNNNNSKKQKQQSKQMSVQMKLEKEIEYRIERKMFSFFVGLCNRYERIEVLDLSGCTDLRMDSLSMLVKHFSQTLRKLITRRCKSPTITDASLADLFKQQYLPKLNTLSIGEMNSVGDLTIASVCLYCPELTALGLARCSKITDQSIINLSQSPIASRLSIIGLNHLSHLQDESILALGMSSPNLSIFSVSNLKNLTSDTLSKIFLNCNLLGSIDLSGCVNIDDTVLDSISMRCHQLFQLNISRSTKVTSLAVKRVVQNCKKLSILFLCKTTVDDECILSIIENLNLLEILYASSCCNLTDTSVGAILNLGTERLGKMIVSFSFCPNISKSLLSILHSFTKTSTSPSLSSSCSDSLEDKEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V350</accession>
    <name>A0A8J4V350_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF6314 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009278</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000669">
        <property type="protein sequence ID" value="KAF2069404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V350"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045632">
        <property type="entry name" value="DUF6314"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19834">
        <property type="entry name" value="DUF6314"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6314" evidence="1">
        <location>
            <begin position="8"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="173" mass="19697" checksum="1537668181D5D1B5" modified="2022-05-25" version="1">MFKIFQSFAGRWRIQRDIVHRTTTSIGTNSGFFSSSDSSNKCMTVNGSATFSPLIGENNTTVQDAYQYSEEGVLRQPDGQSFNVSQKYIYKLKDDIISVYFDEKPERILHTLEFKDSSLSSQVATGHHLCGCDTYDATFHFTSPQEFKIIYTVNGPKKAYTMTTKYFKLEQPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3U7</accession>
    <name>A0A8J4V3U7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008511</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000531">
        <property type="protein sequence ID" value="KAF2070169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3U7"/>
    <dbReference type="OrthoDB" id="550575at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:002875">
        <property type="entry name" value="F-box/leucine rich repeat protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="31"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="490"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="490"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="563" mass="64102" checksum="3FDC4B57CBDD467B" modified="2022-05-25" version="1">MDAFSCFGRNKKKAVSLKQLQAEEKEAQQNPNFFNLLSTELILYILKYMTIPELCNIARTSRRFKECCYDSTLWRTLAMTNNMQKKKINSALKNMESTSRLSSVRMINLNNEHIKQQTIDYIVTHTPQLKEIRLHNLRLTEKTSKLLVAKCPNLEQVYMDGGRTSDECLELLANGAVKLKSINLHKVENITTTGICHIIKNTNLSFLNFNGISGWDIRTLAPYCAHFTSMDLGSSNNLSDDDLKALTRQCKKLKFISLKSCKLITDHGVLELIHDCPQLMDLNLASCSKVTRTSVQHVLQQLHSLTTLNLSCFKNIHPIIFPKNPYRLLNTLTTIDLSYTDVNDDDITQLTEYAQNLKNLRLCACVEVTDTSMILIATHCKKLVCVDLSRDQQTMGTGSSHNKGPMKITLETVENLFLNCPDLQKVALSYSSKNNITKRSVMEVHDRRTSYGWKNINFTLNEEIVLHHSNTNNNNNNIINNINNLNIPLNNNNNNNNNNNNLNNNNNNLNNNTNNNNNRDSFVPNLDRLVDPIQHNGSNNNNNNIPYINPNIIPQNNIPIQNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5V3</accession>
    <name>A0A8J4V5V3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006553</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000308">
        <property type="protein sequence ID" value="KAF2072134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5V3"/>
    <dbReference type="OrthoDB" id="18744at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2072134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="107" mass="12135" checksum="9877256AFADDE5EA" modified="2022-05-25" version="1">MESKVNNLKINTSLVNNNNNNNDIHIGTIENKNISPLSSPKSAREPISPRYANLLKNKDIHHKLRHKRYFDSADWVLNGGNIPTTEEDETLLNAAFYPNKMMTEAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5Z4</accession>
    <name>A0A8J4V5Z4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003394</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000105">
        <property type="protein sequence ID" value="KAF2075317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5Z4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035298961" evidence="1">
        <location>
            <begin position="20"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="307" mass="34007" checksum="BF3A3504C8B99C83" modified="2022-05-25" version="1">MKSITICLLLALCAYYINARTILGYGDLAANNIYTIDNVNNIKYSKAYDPQPQFMLAFGGLATNASYSFNAVYTNGDQTMTWGLVNFMKGTITDLSTIHNAYPVNGLSGLSLYVGNGTLYYLGSQKTSGGNQIDFIQSNFHTKDQTTLPITISAYPAQGIYDFTSGADYFVVTITNSNQYECFWVDVITNQVIQNVVLPATSFVDSKFQIFAYQRNLYIVKSDMAGKYVEVYIVDWTNQALNLYARVQVPNQPISLQVSVIANYLAIFSTGRNDQTPTIPLINLSSSLVTDFLTGLSVSNADTYFIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V777</accession>
    <name>A0A8J4V777_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000056">
        <property type="protein sequence ID" value="KAF2076577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V777"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="68"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="130" mass="14787" checksum="E490F12E90D05F93" modified="2022-05-25" version="1">MGNNSSRSINFNSNIDEEIASLQSIIFNPGKDEIESEKKYNEFLRAKNGSFFVPTSQFNINFNLNENPNSINNTNNHINNNNNNNNNNNNNNNNNNNNNNNNNNNNSNNNSFPRTQNKKKNPPLTLIVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7T2</accession>
    <name>A0A8J4V7T2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Rho GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_004388</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000154">
        <property type="protein sequence ID" value="KAF2074294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7T2"/>
    <dbReference type="OrthoDB" id="13641at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00157">
        <property type="entry name" value="Rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000983">
        <property type="entry name" value="Rho family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2074294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="201" mass="22059" checksum="79939A0EA1ACC623" modified="2022-05-25" version="1">MVKDIKVMVVGDMSVGKTCLLISYTTNSFPGEYVPTVFDNYNANAIVNNTPINLGLWDTAGSEEYNSFRPLSYPGTDVFIICFSLISLSSFENVINKWHPEIQSNMETIPPIILVGTKLDLRNKQKSSGSEGIVTGEMGEQMKGAIGAYKYCECSALTQDGLTTVFEEAGRVVLFPPSKEELAAATKKQKDPKDPKNCIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V858</accession>
    <name>A0A8J4V858_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001191</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000027">
        <property type="protein sequence ID" value="KAF2077492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V858"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="556" mass="64323" checksum="9921239FFA51E298" modified="2022-05-25" version="1">MISDHLILQKIIQKSINQYETKQFIHFSHFHYTLSKLMVISKSWLLNILPKIKYPLLLVNSEDEWKKVVNFIVNHKLPLRLSFSYQGIQFINSLAQPSLFQRSKQLIQRFMQLFSSSPDSIMTIDRFINTIGPHFDTVLLNEHLQYLYRPDPLELYFKSPELLSNLRVYKGEFTFHSKDSLLLKIPSQYRDSITDMHLRGYGDYDIEKYKGIIPTMKNLKNISIEDYRINPSLARIGSLIEDIVQNEMIETVSIDIHSFSPTFSALTKPTTLVLGESLSIQQFKTIIDQCLSLVSLYVSLVSVQDNQTSCFKISHKSLTTLSVNNLVVWKLCTFSVPSLTNFNMRNNKKKRCVFTSSGDLNGLLIQFPQLDKLSLSNCHETSKGNSNRSSFQYIVSKNISNQFFPRTINQLSFYSFSFTMSQVTVLFRQLSKYKQINDLEISHCNQRTQGQTHTTMEFNEMLEALAQNTQLAKLFLNDNNFTNQDENSLILFKVLECLPLTHLAFIEPTSCLHTDEGLDKVKLYIRNNTDLLRLNLRAAPHLSSFFNSRGIVYLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8P9</accession>
    <name>A0A8J4V8P9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]</fullName>
            <ecNumber evidence="9">1.3.1.88</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_003413</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>5,6-dihydrouridine(16) in tRNA + NAD(+) = uridine(16) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53380"/>
            <dbReference type="Rhea" id="RHEA-COMP:13543"/>
            <dbReference type="Rhea" id="RHEA-COMP:13544"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:53382"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>5,6-dihydrouridine(16) in tRNA + NADP(+) = uridine(16) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53376"/>
            <dbReference type="Rhea" id="RHEA-COMP:13543"/>
            <dbReference type="Rhea" id="RHEA-COMP:13544"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:53378"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5,6-dihydrouridine(17) in tRNA + NAD(+) = uridine(17) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53372"/>
            <dbReference type="Rhea" id="RHEA-COMP:13541"/>
            <dbReference type="Rhea" id="RHEA-COMP:13542"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="10">
            <dbReference type="Rhea" id="RHEA:53374"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>5,6-dihydrouridine(17) in tRNA + NADP(+) = uridine(17) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53368"/>
            <dbReference type="Rhea" id="RHEA-COMP:13541"/>
            <dbReference type="Rhea" id="RHEA-COMP:13542"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:53370"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Dus family. Dus1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.88" evidence="9"/>
    <dbReference type="EMBL" id="AJWJ01000106">
        <property type="protein sequence ID" value="KAF2075284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8P9"/>
    <dbReference type="OrthoDB" id="272303at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017150">
        <property type="term" value="F:tRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082">
        <property type="entry name" value="TRNA-DIHYDROURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082:SF5">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(16_17) SYNTHASE [NAD(P)(+)]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="15">
        <location>
            <begin position="53"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="355"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="356"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="377"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2075284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="408" mass="46451" checksum="B0027C274395E3CA" modified="2022-05-25" version="1">MESINTTVDQQQQQTEEQEQQQIVSIPEPMSPITKLGGWEFYKTVLKSPKKVIAPMVDHTYLAFRMLCRKYGADLVYTPMFHSKNFATCKVYRKESFSTCPEDRPLVVQFCGNEAEWVVKAAKFVEDECDAIDLNLGCPQQIAKRGNYGAFLLEKPEIFLPIVRELHKNIKVPIFCKIRLLPDINDTIKLALSLQEAGCQLLTIHGRTKEQKGQHAGIANWEAIKIIREKLSIPVFANGSVVLYKDIEPCLEATGAQGVMSAEGILANPSMFSGLDLPITQIAKEYIDMCLVYHTETYICRSHLFKMLRSLFDYHVDLREKMGKIHTFEGFRDLITELEVRIESGIQPEKMLTNKQKRELKEKEREDELKRQKMDTTEIITTTTTDDKPNTTVTETPTTATTTATVST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8X3</accession>
    <name>A0A8J4V8X3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Signal recognition particle 54 kDa protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_000418</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The M domain binds the 7SL RNA in presence of SRP19 and binds the signal sequence of presecretory proteins.</text>
    </comment>
    <comment type="domain">
        <text evidence="13">The NG domain, also named G domain, is a special guanosine triphosphatase (GTPase) domain, which binds GTP and forms a guanosine 5'-triphosphate (GTP)-dependent complex with a homologous NG domain in the SRP receptor subunit SRPRA. The two NG domains undergo cooperative rearrangements upon their assembly, which culminate in the reciprocal activation of the GTPase activity of one another. SRP receptor compaction upon binding with cargo-loaded SRP and GTPase rearrangement drive SRP-mediated cotranslational protein translocation into the ER.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000008">
        <property type="protein sequence ID" value="KAF2078227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8X3"/>
    <dbReference type="OrthoDB" id="10250817at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030942">
        <property type="term" value="F:endoplasmic reticulum signal peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006616">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17875">
        <property type="entry name" value="SRP54_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000022">
        <property type="entry name" value="Signal recognition particle 54 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="entry name" value="Signal recognition particle, SRP54 subunit, M-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006325">
        <property type="entry name" value="SRP54_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01425">
        <property type="entry name" value="SRP54_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 54K PROTEIN SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564:SF5">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="Domain of the SRP/SRP receptor G-proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="Signal peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10 13" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="14">
        <location>
            <begin position="269"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2078227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="507" mass="54546" checksum="ECB6282E7CA70A51" modified="2022-05-25" version="1">MVLGDLGNQISSALAAMSNTTIINEETVNNLLKEVGNALSKADVSMNLIIQMRKNIKDKIKLDQMAAGLNKKKIIREVVFEELIKLLDPGVPAWKPVKGKPNVVMFVGLQGAGKTTSVTKLAFFYKSKGWNTAMVCADTFRAGAFDQLRHNATKAGIPYYGSEEVKDPVMVAKKGVEIFKKEGAEIIIVDTSGRHKQDNELFEEMKQIETSIKPDNVIFVMDSSIGQAAYEQATAFKTSVKVGSIIITKMDGNSKGGGAISGVAATKSPIIFIGTGEHIPNLELFNAQTFVSKLLGYGDLSGMFNKIQEVIPEDSQSLKEIASGKFTLRSMQAQFQQILKLGPIDQFIGMIPGMNNMPQLQGNEGNLKLKAYINIMDSMSEKELDGKKPINQKRMLSLAQGSGRHPREIYELLEQYKAFEKMIGTKGPGGFSNLANMASKGGVPNAKNLQQLANMVPPGLMKQMASGGGMANILNSLKGMKGMGDLSKMMGGAGGGGGGFPSGFGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V9J8</accession>
    <name>A0A8J4V9J8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein bL21m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_002484</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000069">
        <property type="protein sequence ID" value="KAF2076184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V9J8"/>
    <dbReference type="OrthoDB" id="5994at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01363">
        <property type="entry name" value="Ribosomal_bL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028909">
        <property type="entry name" value="bL21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036164">
        <property type="entry name" value="bL21-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001787">
        <property type="entry name" value="Ribosomal_bL21"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00061">
        <property type="entry name" value="L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21349">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21349:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL21M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00829">
        <property type="entry name" value="Ribosomal_L21p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141091">
        <property type="entry name" value="L21p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2076184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="168" mass="18975" checksum="19DB907C42862CF4" modified="2022-05-25" version="1">MMMNRFSNLFMRSSSLMNRSYTTTHKSSTTTTTMATASAPTTVKLIDESEYNQKHSFTAIENSFAVVHVGGKQYKVIKGDLIMTDRLPTVEVGEHIVLDKVLLVGTQDSTTIGTPLAEEYKVHAYIEEQSKSQHVTIFKHKRRKNYKRTTGFTPLATTLRIGEIIKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VAU3</accession>
    <name>A0A8J4VAU3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000026">
        <property type="protein sequence ID" value="KAF2077519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VAU3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="610" mass="69699" checksum="137B90DBE323DC5E" modified="2022-05-25" version="1">MDNLFFSIWRNNVTRDSIRTMVLDGKIITINLPYLFENYRYLSKVDRSQTHIKLIIYPHHFTKFNDQFVHYKHLVTYIKFSKDFNLPLPLDFFPLDLKILDFGLGFDQELQVSQLPSNNLHTLYLSHYFNRKLKGKVLPDSLRNLYFGDIYSHPLEVNILPKQLEFLSLGLFSPPISNVQVLPKSLTKLCVSNSKNQILSPKVFYETSLLDLTVGGVYQDPIVEPLFPKNLESLVLKKFASPIKPFLFPNTLTTLNINNFYPFGQPIFKNSFPSTLTTLFLNVQDSELNEQDGFLPPTLTNLHFFFKSKSVSSKLLPRSIKYLKIGINGLHQPLPDEFFPENLLDLELLDFELPFTTRKMIPNKVVNLTLGCSFIQPLEIGDLPESIKKLQFTSLDQPIEKGLLPSLLTHLVFPNSHSFMIRSGSIPNSVKYLILPLSCGNPPNRLKQVVPLSVKRLTIIGNYSHLPLEKDSIPPVKEIVISCLNCKLLPGSIPSTAKRLVFRNYLSIIDKGIIPDSIETLEFHDPFDQPITSDLIPRNLKKLVISNCPTNPKIPSEILPPSITSLFIPWMNIKGRIPPSIKKLYMFTKQSSLVEIPDSIIMFNEAFDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJQ0</accession>
    <name>A0A8J4PJQ0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MACPF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009759</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000798">
        <property type="protein sequence ID" value="KAF2068920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="A0A8J4PJQ0"/>
    <dbReference type="OrthoDB" id="22524at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00457">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="MACPF" evidence="2">
        <location>
            <begin position="128"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1815" mass="207071" checksum="EE946CB2B9D333C3" modified="2022-05-25" version="1" fragment="single">MGFKLIVDSSLVINSYFQQPEFSTIRGIKLQGGMHFIKLVFFSTASDSRNISLEWRPSNQENFNLVDGVYSLNNCNDGILDKDEIDIKSKFYCKADLSSINPNAEPVCGDKICNEPTENCIADCYHLITPLCPGQAPPSPIFFEKKDTVGELLDNQYLYTLPGIHHLRHGVDAIIGAQKEGFVYTLPKGVFGTISPTCKFDAKTEFYESSSKMSSDMAESSGLSTSASASGGSLFIKVSLSASYSQDESVKEAKELESEHSGTIAQTTVGCEISKVHVTQHDFSQEFLRELGYPTTVDQMEKVIKRFGNTYYQSATLGGSLTQITLIKDSYTKGKSQTETKKHIDYSLSASVSSPVFSVSGQYSRSDDVDFTSESLNEFREGTESSRTITKGGPPGAFSPSSSSYQSWAQAVDLLPVPIKYKLGYISDILPSNWLVQGSETGLTIKQLWIAAEDKILNHISKLPVVLSRDKTILSVSQILFVSDPLNGVNIDTTLQSPLEFYDYAYSRFYQLRKNNNVIGGINSYLKPNAIYVRLHLLSFLENFKPFGHILHIKLDSITPNKTIYYSLADTPYFRGQKQNIEIQLSSYIGPLVSIEFNYWSINDEYNRFISYIRDELNSTNDLVIKDSNSSELLQILSVMNEINNITTPIIDHQQQQQKQQFNLVLLLNINYNDQYDDNIISIITELKDYIQSISIQMYSDNKDKETLEDNIKRRKTETKDDTNSDKNRGILLKQIKKTIHLTTTMIKIDQLQSYIYIDSFFLHNYIYNDMKNFQLSNKPKFQGLRQVAPPKTIPTGVIGVHFIDVDPKLPFTPIPNSVIFLKIDEFNKPLSEINFPPYLKFLSLGRFSQFIYRNLDIPSSVTHLEIDQHGNTIDNHMDHYAYHWLFCKGTLPRNVTHLKIIDPPITEKISTPILIPSSVKYLDFNNNINYYNIQIIDENEYYKEPITKDSFNNLKTLKSVKFPKIAIWVKKEGHPYDYESYPETQIRLGIQPIITTNLFPKQLESLDILEHINQFDTSLLPSSLTSLTCFSPSNVPNHCNLHYLSVSMIELDEKMIFKSPDNKPITQDQIPQSFNPTNGQFTPEPQIPLDALYLSTDYICYRSALVPKNNIIIKKLVCFRQDLPLPDSVEYYRAYVIPHSFPSSLKTIHITSFLGDSGIPPSVGFKRDPNWLHSLPPTVKKIVPLLDYSEAPMFPEIVDELLKDKYKLNHENNSINSINSIVSNDNFLKVWRNEWLKNHIYSFIQPKLIQFVQNQDNNNNNIVFNFTESFCSNHIKLRTRILCIPPNGVTYYSVISIQKKEDLFVQLPSSIKKLRIDYQGLSKVGIPPSLKHLILKSQDSELILKEQITIPSNIEILEFGNTCFGIQDLKKLITPNIKQIILSQKCLRGTSVNTEILTFLYERNLLKNHRYQIYEPNVPISSKTSVLIWRLDRVIPIGIIPFGVRRIIFGDNFNQTILENSIPTSVVEINFGTDFNQFLSNIYLPISLKYLSYSSRSWSPNPMVSSSLPPNCTHVSIKWPKYKCTGYSPYCKSLAQTPKFVKFINVGAYKFKQDYNNDSKKFKIQSFYSNEEKCCEISKYIISEANKNIDIYHQETIEPKQLNTSIKSNHSLEIKLKIKRNIPLLPSLLDGSNVTSIKFGPYFNQAIVNGSLPTTVSRITFGKYSSFSQQMLVLPPSITYLHIRHLTRFHEFIESFVFPPSLTELYLPDNFNESIPNGVLPNNLKNLYFGECFSQKINIGTLPLGLKVLSFGKNYNQIIDKTVIPNTVEELYLYQRHESTTLDFIPTSVTKLQIESHNLEQILKTIPPTVNHLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJS5</accession>
    <name>A0A8J4PJS5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">IPT/TIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_010492</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001149">
        <property type="protein sequence ID" value="KAF2068182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJS5"/>
    <dbReference type="OrthoDB" id="24520at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052014">
        <property type="entry name" value="Dictyostelium_Tiger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31341:SF15">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31341">
        <property type="entry name" value="IPT/TIG DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035274227" description="IPT/TIG domain-containing protein" evidence="3">
        <location>
            <begin position="23"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2068182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="746" mass="80922" checksum="21C80E4B65903E0C" modified="2022-05-25" version="1" fragment="single">MIKTTPFIFFYIIASFVLCVNAVPPTITSLSRVWGKINADLTITGSNFGSSTPPVWVNNVQGTSTASSTSAVFTMFGTSPDKTYNIQVSNPANEKSNTLTYYYADIVDFYQNNKIFYMLGDFEMIPDKTILRMKPNSGTTFYPITYINNTHLSLPMVEELNGDIIWVNFVNYYGNDPYGGGAVSFSPVVSAYYFTVDGLAITGFKYIPNTVIQMEDSVNCELLSQTIYGMQCAPPPLSLANPVTQFIVQYPTGKKTSIPISFTPLTGFNNIADNKTVLSVTNFDTSALSIKINGLSATPITSSLTKSNEITFTYPINAQCGYTFVSSGVSRITNNLLVCPNPVFDSISKPFESSLQLIVRGYFMNPIIYNIGVGGVSYNYQYDDGVEVPSDCIQNMIFDPSDSSYTFTCTLPDLRPFNFIAKTLTGYTNVFYFGYYPIITSATSTNYLFQGVVTITGSRFTNFDLSTSIGGAPCTSPAVVANTGSTKITCVFESNVLVSNFKQPLPVTVSTRNGFESTAAVFRYNRPDPIISSVTSANYGTPSGITISGLYLFSDVLYISIGGVECELLTVNAEGTKILCNFPSTASPNGYTAPLQVHVSVEHTYNTTAPLFYYLRPIPVVTGSTSTTYGIAGVITVFGNYFTPPPPTSLQVIIGGTQCASPTVVSETMIACVFSSNVVVSNFKTPLPVTVTIDSRYTTSNSVFYYIRPNPVVSSATPTKYLSAGQVTITGKYFSYPDSLSVTIGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKB0</accession>
    <name>A0A8J4PKB0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_009262</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000666">
        <property type="protein sequence ID" value="KAF2069415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKB0"/>
    <dbReference type="OrthoDB" id="118154at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03572">
        <property type="entry name" value="ENTH_like_Tepsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035802">
        <property type="entry name" value="ENTH/VHS_tepsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039273">
        <property type="entry name" value="TEPSIN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21514">
        <property type="entry name" value="AP-4 COMPLEX ACCESSORY SUBUNIT TEPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21514:SF0">
        <property type="entry name" value="AP-4 COMPLEX ACCESSORY SUBUNIT TEPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="6">
        <location>
            <begin position="7"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="407"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="496"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="562"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="407"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="503"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="562"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="594"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="621"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2069415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="692" mass="77266" checksum="6AD08E50CF8F4810" modified="2022-05-25" version="1">MNNLITNISNNIQSVISIVGKATPDNTEPTPGYLFVDLQNETFKSIEKCTDILDQLLIRLKKDSPHVKLKTLKIIKHLVVKGNIIFKQEISTKSELLHQCTYFRGPANQDETTSYSLLVRKEAQDILNLLFQFSSDNRAMVPVDSSQQDDYSRGMVLYDSNGERTGNRIIGFQNNGSATNAPVQTPTKIDEIKQKITSLPSKIISMESRKVTLSTAPDIIDPASYAMVPIKDGNHLMSYTATGGSKETFIFTQEELKNLSKTRHYDTIVYQRLSTIIIGADSVSALQPNLSNESVSSFIDYFLSLGASDQHSVFEILDDWLIYYKKWQLKQKTLILVEQLLLFNQSLAIEYYGTNHTNVGALLVSIHQSLREKSKSILNQLNVDLSIYKKEKEKELIHPPTTDLSQLIQQPQYQQQPQQPTTKHPNQQQYSNLFNFDDSSSPSSSTVKKNQDSNIFGGLSMKQSTSQSNLLDLDFNSTGSTANNINDNNKSTSGSIDMDFLLSNNTSNSNNSNNSNNSSKNNNCNNTSKSSIMDDFDLSSISFTSNTNSDTLVASPAIKTMTNNNNIDKTNTNNNNSFSFDTHDISTSLKPISNNNNNNNNNNNNNNNNNNNNNNNNNNNKPTTASTINSTPKPSPNYNFTFETLSPTNISPLPSYSNQQKLNPQNQTKPTTTVIDPFQNLTETYIAKNKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKB4</accession>
    <name>A0A8J4PKB4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009341</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000683">
        <property type="protein sequence ID" value="KAF2069342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKB4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="581" mass="68568" checksum="80F73E3F4F132DD7" modified="2022-05-25" version="1">MVKDHLTVDCLKLALQANAELKVIQFLVTNFPTPNAQYVFEIWKSCLIGGSVENFIYIKRTIHQREYVYIYSQFMEEAIDMERWEMVELLLRQDSNPRSQYDATRLPLHVIKNEIERHREQCFSTQALNKSLAGSFQSPDPKPKLVVPRLPPKYLDTVPLLLSSQLPFTEFLELDFRKGAKLGYVPLLRFMAILSKPSRNDWDLSKNRETIDFFLTFFPEIDWDFNGLAVNNGDLELVRYVFLEKNICNINSEIYNSNHQVLDFLRDQQVLYGIEFKDSLITNSFHGDYRILEIVYQTCNQPFISQQLFDKCVKDNRVDLVRYMLLNPNMLISIENTNYSFSKIDLKTSSEMIQLLFDFSNKQQLKQVFDSFKNSQIVLLIMGGHYQIVQLLFTLDPQNLGVYLRGINSPIPLKMCKFLLGFPQQEIYQYIEYAVNRSYYKLLQFIYSTYSDLNYWMLDNPIFNQRSLLEIINSSTKIKDLINHWETYGSLLSLKYYISTQLYEIDQQIALLNYIFRQLSLKKIVKLFPEISSFNVPIFKEFQLFIIKQNLISNFELKSIKKSDILVLKPTIDIFIKEYNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKD2</accession>
    <name>A0A8J4PKD2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010315</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001034">
        <property type="protein sequence ID" value="KAF2068360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKD2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="242"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="259" mass="29562" checksum="AAD0DDAD2EF23524" modified="2022-05-25" version="1">MPLHYTTISYFADYCDLCANKRTKRLAENAINLLKQKSAIDGLSHLHPPRQHSTAARALMKEKLNNWLQEFNFFDYDTLYGPMHHVLDISFSISSLGCDQDDDHSLFQISTTSTSVGEQRSLVDHDQDSSNNGPSLFSSQILNTFMDEDQDDVDNRYEVLSIGDSSVSNFDDVDFILDNPSDDYVSLIDKETVLYNYNNEQYLVVHSDEEYEDGESISSSFFNSFSSSSSCLPIDTHFENGIAQYSVLVFMFIIWIFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKJ7</accession>
    <name>A0A8J4PKJ7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">AMP deaminase</fullName>
            <ecNumber evidence="4 9">3.5.4.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_010196</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>AMP + H2O + H(+) = IMP + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:14777"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.5.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via salvage pathway; IMP from AMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.6" evidence="4 9"/>
    <dbReference type="EMBL" id="AJWJ01000967">
        <property type="protein sequence ID" value="KAF2068480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKJ7"/>
    <dbReference type="OrthoDB" id="1723809at2759"/>
    <dbReference type="UniPathway" id="UPA00591">
        <property type="reaction ID" value="UER00663"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003876">
        <property type="term" value="F:AMP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046033">
        <property type="term" value="P:AMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01319">
        <property type="entry name" value="AMPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.800.20:FF:000001">
        <property type="entry name" value="AMP deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000035">
        <property type="entry name" value="Probable amp deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006650">
        <property type="entry name" value="A/AMP_deam_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006329">
        <property type="entry name" value="AMPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01429">
        <property type="entry name" value="AMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359">
        <property type="entry name" value="AMP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359:SF0">
        <property type="entry name" value="AMP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19326">
        <property type="entry name" value="AMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001251">
        <property type="entry name" value="AMP_deaminase_met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00485">
        <property type="entry name" value="A_DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="686"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="709"/>
            <end position="736"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR001251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2068480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="763" mass="89051" checksum="F930EC63C203B3B1" modified="2022-05-25" version="1">MSTPLRGSSPQVSFYDQEYFEEEANEDSSQFTYREYMEEDKINSFTYNMAKKDSTNVFQRIILTNEHEKDIEEFAEVAEQLLDAINLRNKYIYTPKIWKEDATPSEKPPYNPFEPEFTAATEHTYKVVNGVYYVFRNEKEMKDDNPLYSVPHTLSSYYKDINNLMKLCSYGPAKTFCFKRLRLLESKYNMHILLNDSKELAHQKNAPHRDFYNVRKVDTHVHHSSSMNQKHLLKFIKRKLKENPQEIVIFRDDKYLTLAEVFKSLNLDVYELSVDTLDVHADNNTFHRFDKFNLKYNPCGQSRLREIFLKTDNLIKGKYLAEITKEVFCDLEKSKYQSAEYRLSIYGRKMNEWDTLAGWICDNGLFSTKARWLIQIPRLYDVYRETSTTTFQDFLNHIFHPLFEVTKDPSSHPKLHKFLQQVVGLDCVDDESKFEKKFTEKFPVPSDWTSEHNPPYTYYLYYIYANLVTLNKFREEKGLNTLTLRPHSGEAGEVDHMAAAFYLSHGINHGINLRKTPVLQYLYYLTQIGIAMSPLSNNSLFLTYNRNPFPVFFARGLNVSISTDDPLQFHYTKEPLMEEYSIATQVWRLSPCDICEIARNSVLQSGFEHNVKCHWLGPDYVNPNGNDIKKTNISDIRVAFRNETLTEELHIILKSLQTLSNFKELNISFLTEKLPAEIVKDTFGHHHNQHSQQHKEKTPTKSTPINIYNNSNNSNSNSSTSSANSSNNNSKNSSPNYHMQALKHKAKTSMPPPLSLNVSKENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-06-18" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL24</accession>
    <name>A0A8J4PL24_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_009691</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000779">
        <property type="protein sequence ID" value="KAF2068992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="14374at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050680">
        <property type="entry name" value="YpeA/RimI_acetyltransf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43420">
        <property type="entry name" value="ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="4">
        <location>
            <begin position="639"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1443"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1459"/>
            <end position="1483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1509"/>
            <end position="1526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1565" mass="178669" checksum="008670309F399FA4" modified="2022-05-25" version="1">MASVSTPPYSSANSGNENNEKTTPKLSPESDDIIDEEEWGFVDLWETYDYHLLERFWNELVMTSFNDKDMQPLNDWNKAMSPEAHNNPDIPDLHVLLAFKASDLESIKCSTDAYMENLSASSPSSFTTTSSSVISPPLNNKQIAPLNSNSQITAGIIFEYYSNINCGLFLHMIIQKKFRNMGLERLLVKKAIEILDKNAHSHGHLAGCNAIFFETKSAINVPTDTIHNSSQIDPRLQHNILHKIGFRLVDFDYTKLPLKRLGKANNLLLTVYLSPHIPSQSSLDGDKFFLPSVLLKNFIYDLWETSFLTEKLNMKPDEDPEYGRALEQIELREKIPLLDLPWGAGKPWTLVDLWEDYDEELLERCYRELMVPSVKHSEISSLGNVLRAMSPEGDDSSFLPDVHVLLAVRWPTSSDVGKVDQLSLPIIDGLCVFEYYTEQNCGILTHLILQKKGRAADGLDNLLVESTVDILDANARERGNLAGCNAIFIHIKNASSTVGTEVADLCQHHMVLYKMGFKLLDFDYYIPPLDKNSAPKKLMLGLFITPLIPRSNEKGDKPFIPFNLLKLFVELQWNNAFGSEQICKSPDTYLEYQRMIEQIEIRERIPLLDLPWDSGKPWTLVDLWEDYDEELLTRLYKDIMIPNFPNKSELEPLENWKQSLSSECRDDPNISDLHILLALRWVDNSNQPIIEGVIIFEYFSANNCGLLTYLIVQKTFKGKGLDRILVESAIDILDQNAKSQGFLPGCNAIFLEVYSGEKVTIKQDVVDPRMRHAIYHQIGFRLIDFEYIAPPLSITFPKLKHFLLTVYLTEHIPKIPLEEGKYYLPNSVLKNFITVLWKNAFATKKIREPPQNDSDFVRMIDQFEYREKIPLLDLPWGASKPWTLVDLRDDYDQDLMDRFYNELMLPNFPMKNELEPLSNFVTALERETYNPNLSEVHVLLALRWPNESSDLQPTIGAGIIFEYYPNTNCGLLSYLVVHRSSRGHGLARVLVERAVELLDKHAKTRGQLAGCNVILLETNSAEKVTVQQDVMDPRMRHTIYHKMGFRMIDFEYIMPPLFQGYEKLRGVLLLTAYLTPHIPYQDVNGYKQYYLPNVLLKNFISAQWENAYRNGRLSLLPKQDTDFVSSLDQLEIREKIPLLDLPWGDGKDWMIVDLWEDYDQELLDAFYNKYMMTQFGTSDELEPLENWHKALSTEGRDDPNICDLHVLLALGLPGEHKGKGHKYIIGGLVFEYYPETNTALVTYLVVNQRWSGKGIGTDLILRTLGILDVNAKQKGHIAGCNAIFLEVMFSPHELTGGPDSSLSHVFLFNKGFRLLDFEYYQPPTSLKNPKSNNVCLTVLLTPRIPKHGEGSEIQPYVPSSLLGSFITTLWEDECGLIGYNYSNDRCYLRMMEQLEKNEYFHCLDLPWVKYRIDNGIVGSQPSAIQLNREHHFTSSFNNMNINYSSPNPVHLDHRSRLRNSTSNDDWDNNNNGSSSSITTTNSDHNPKINVIRESGSPDDIDNNDKDSNNNDSSLKNSPLNSPVLSPQSINSIRLPPDSTPLSPALYPTLSNSTLIQKSSFISLDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLE9</accession>
    <name>A0A8J4PLE9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008560</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000539">
        <property type="protein sequence ID" value="KAF2070120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLE9"/>
    <dbReference type="OrthoDB" id="22074at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="725" mass="84673" checksum="99660E407CCAB844" modified="2022-05-25" version="1">MDILYRKVFYNSVIYNHIFQYIRDFALQDIKNIDWVIQNNRWKILEYIIDNNYYLQIKDLDLLIGIVDKVDLSLFKKVFNRFKDRFVAGFSDILCHCVNNPEATLFLLELGFKHSGKCMIRAWESKNYEKVKYFLNTLGDKHIDSNINEALKVGNTEILQICLRYYKELYGDQTINIGDLYGPTNIKQYFNKACESGSVENFKLLRKTFPNEPFASNWFRLTTKNQDLLIYFFENYLNRIRNFKEYELDSIFRDYVRCAYENLRLIEKVTSLGFFFSSLDLLNISTSIGVGAFYNQDMVTFNKYQPNPHRILFSSIAISKVQDGFSSVERVMELKEKGCVFSKLCFSKAVAGDRVDIFEKFATDSLINDIEPSILLLYRCKNILAALIKRGVKLRGYGAFEASWNFEDHFDFLKELWTFNPPTIIPLTSFLMISIQQNSTKVFRYLYETFLKEKSETIDRLNLTLYNHALNQPNSDIIECMVQMKIEKSFLITLGFASSTKQVQILFPKPFTPTNTDILVNSYKNSLPNLDVFEFILSKRFINVSNEVIGKSILVAINADQVAHFIFLLDNYSVFFEHWDIEIPVLKSGNLEIFKYYITKYPSTNLDQNVLRFKQAIKFQSFDIINFMLEAIDPTLGNTLLAKDFELEESQFFFGNPGFGRPTISIDWRIKKAPLFKCLSKYMDKKSLVSLLNSLKGTLAPPEAQNRQFLESFIHYLVTNNNNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLG3</accession>
    <name>A0A8J4PLG3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">COPI associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_009518</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000730">
        <property type="protein sequence ID" value="KAF2069158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLG3"/>
    <dbReference type="OrthoDB" id="423534at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013714">
        <property type="entry name" value="Golgi_TVP15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28128">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28128:SF1">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08507">
        <property type="entry name" value="COPI_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2069158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="146" mass="16319" checksum="79F24282DFB48FAB" modified="2022-05-25" version="1">MARTNVSLIFSILGVLGGLFVLAVGIYLFVTGLWHSIIRFVLGCYYLLFGVCIVLFEFIFPSIMVHLFGFYTYYFGKGTLFITMGLLILENNGFFLAAGIIVIAIGIVFCIAHFVFGRPSPLVDRSNGDVHHNRHPNEQDPKHQTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLH7</accession>
    <name>A0A8J4PLH7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008522</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000532">
        <property type="protein sequence ID" value="KAF2070165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLH7"/>
    <dbReference type="OrthoDB" id="206700at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1310:FF:000005">
        <property type="entry name" value="GTPase-activating protein gyp10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000100">
        <property type="entry name" value="TBC1 domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045913">
        <property type="entry name" value="TBC20/Gyp8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20913:SF7">
        <property type="entry name" value="RE60063P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20913">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 20/GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="328"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="40"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="398" mass="46337" checksum="A131135E108F23CA" modified="2022-05-25" version="1">MFTNKERLEAKKVREIQQCLEQTPIDIEGLRRCSISNFGFVNNGLRRRVWPILLNIDTEHIINHNSKIIDHKDTDQVLKDVERSMWRFTKGESQLRNQKKPELMRIVNAVLSIHPSLYYFQGYHEIASVLLLITDESLAFAMLERLSLNHFNDCMLPNFSEVLKVLNLLFPLISLMDQNVYNFLLKSNVQPLFSISWILTWFSHNVEELDHAARLYDFFLSSHPLACLYFSAALIEHLKTPLLRLECAYDTVHGFFSSNLPQDLPLDKIIQQTQYLLDKFPPTKLQMKTNQYLTKSSPMNMYPYSWMQSSIRAKGKGKKNNSDPTQQIYKWSFIILFGSISIFSILVFLSVKYPNLNLSPIVKNMFSKSTLFSWFSTFGNSNSNQLNNSFPSSLLFDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM54</accession>
    <name>A0A8J4PM54_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_008130</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Phagosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000476">
        <property type="protein sequence ID" value="KAF2070555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM54"/>
    <dbReference type="OrthoDB" id="7862313at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030670">
        <property type="term" value="C:phagocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004445">
        <property type="term" value="F:inositol-polyphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034485">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09093">
        <property type="entry name" value="INPP5c_INPP5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04380">
        <property type="entry name" value="RhoGAP_OCRL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000132">
        <property type="entry name" value="Inositol polyphosphate 5-phosphatase OCRL-1 isoform b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000040">
        <property type="entry name" value="Inositol polyphosphate 5-phosphatase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000012">
        <property type="entry name" value="Putative inositol polyphosphate 5-phosphatase OCRL-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048869">
        <property type="entry name" value="OCRL-1_2_ASH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037793">
        <property type="entry name" value="OCRL1/INPP5B_INPP5c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047078">
        <property type="entry name" value="RhoGAP_OCRL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF300">
        <property type="entry name" value="TYPE II INOSITOL 1,4,5-TRISPHOSPHATE 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21310">
        <property type="entry name" value="OCRL-like_ASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="10">
        <location>
            <begin position="527"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2070555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="725" mass="82700" checksum="2ABF122802F54323" modified="2022-05-25" version="1">MSDTTTNNNSNSSVTTPVTPLVLEDENRGVSDEAIEASLDGLKHNTLKTSYTVLNTNNPHIDVNTQWIHNKLKEKEPEYTEKKNMSIFLGTWNVNGKKPSEPLDLWLKDPQMTIQPDIYAIGFQELDLTAEALLLGDTTRSLPWEQHILSSLQGDYVKLLSRQLVGILLCVYVKREHKPHITNAMSDIAAVGIMGMMGNKGGVAIRFSFYNTTICIINSHLNAHLDNVLRRNQDMRDIAKNIKFITDDGKTINIYEHDQLFWIGDLNYRIPLPDQEVKDRIKKKDFYNLFLQDQLYTQMKSGSAFEGFQEPPISFAPTYKYDAGTDEYDSSEKKRTPAWCDRVLWKTAKTAENVGILSYRRHELMTSDHRPVSASFIIKIKIVIPEAKSRVYQDIVKELDKKENDSMPDASISSNMVDFEEIKFMTPVTRQIVIENIGQVIARFQFIPKLDETSLCKPWLKISPIAGMMIPKEKIMLELTIYVDNLTSQAFNVNGESMDDILILHLENGKDYFISISGVFQKTCFGNTLENLVRYPHPIRNNIPIPSDQKKLSIPKELWRIVDYIYYNGLKEEGLFIESGVTKEMELIRDCLDSGEPFTNITFSIHSMAETLIRFLESLVEPVIPFNMYQQALDASSSPVSCKTLVSHLPAVNYNVFFYLISFLIEALSHNQENKLSPDQLAIIFSTVFLRPSPLSPQSQYPPEAAIVKKRADLILHFLISKDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMB4</accession>
    <name>A0A8J4PMB4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SWAP/Surp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_009024</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000619">
        <property type="protein sequence ID" value="KAF2069663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMB4"/>
    <dbReference type="OrthoDB" id="20674at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.790">
        <property type="entry name" value="Surp module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051485">
        <property type="entry name" value="SR-CTD_assoc_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000061">
        <property type="entry name" value="Surp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035967">
        <property type="entry name" value="SWAP/Surp_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23140">
        <property type="entry name" value="RNA PROCESSING PROTEIN LD23810P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23140:SF0">
        <property type="entry name" value="U2 SNRNP-ASSOCIATED SURP MOTIF-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01805">
        <property type="entry name" value="Surp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00648">
        <property type="entry name" value="SWAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109905">
        <property type="entry name" value="Surp module (SWAP domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50128">
        <property type="entry name" value="SURP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="392"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="SURP motif" evidence="5">
        <location>
            <begin position="532"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="CID" evidence="6">
        <location>
            <begin position="633"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="473"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="607"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="781"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="930"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="94"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="130"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="161"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="306"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="331"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="478"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="788"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="821"/>
            <end position="833"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2069663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="951" mass="110818" checksum="FD0C780295A60A2D" modified="2022-05-25" version="1">MSSKRDDRDKDDGGDEEEKKIMPWGIKEKELAPSTMSKVSQEKIKELTGGIAGNKTQLMQKRKQAEMDSKKKKDESEAAKVYKDFLQEFSEEPNNSGSSRSSNRSAPKFVKGESFVLNEDNQDTEIVQDNSRDEKIKDKDNSRYDRERNDNRDTKNSNSRDYNRNDRDRDRDRDRDYDRNDRNDRDSRDNRDNRDNRDRSDRNDRSDRNDRSDRNNRYSDRDYRNDRDDRDKRDNRNDRDGRNDRDSRDNRDSRDNRDSRDDRDSRNDKDRNDRNDRDSRNDRNDRNDRGDRDHRDNRSDQKRDISSSTNKTTSSNSNRNRFIQDDDDQDTNNINNNNNNTNTPTNKKGKRLIDELKEELKQSQLEKDKEKRIKRDTKGFDNDEGEKRFDYTTLFLGTLSAEANEDVIRSLFSEYGKITTIKIIVPKSEEDRRRGINYCALVTFSSTKDANNAMMNMEGKQFKGREIRMSFAKANPKSSSNNSNSSGITSSSSNKSYNSTSTSSSSNDNNSHQAKNDRIYVKIPTNLSIKTIIDNMAYFVAKEGFPFEKIVMENESSNANFAFLYSAQLEESHYYSWKVFSLLNGGTLTSWKMTPIQMISNGPTYYPPQCDSKESHNSTTQQQPLLQPQQKDISIPTRIKLEDLINHITVSREKIKEAMVFSIDNSDVSCDIVDIIVLSILNNSNHPKLKIAKLFLISDILQNCTVSVQNVSSYRGLFENKLPTVLSNYNQFYKSITGRVTAQHFKDRIIQVLNSWNQSSIYPKNFILGLKFTFLNNNNFNNNSSSSSDDKDKEQDPKTTTEQVEVDDDIDGVPLNRDTNNRNSSNNQQQQQQQIDLYCKQNGLVKSINVQDTLLKIEKFKEFMKQEESSETLTSIDSVDQEKINEEVEEYKKRLMFSSKSMTKEAIQENVKQYRDSLITNQLRFKSLKRELDHGHYNRSSTRSDKRNKFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMK7</accession>
    <name>A0A8J4PMK7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008758</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000573">
        <property type="protein sequence ID" value="KAF2069918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="22524at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1353" mass="154144" checksum="6DF158E758598225" modified="2022-05-25" version="1">MSSSYLIKDDSLFITQNHAHQFEWSVLKTDNCNNNSNNNNNSHNDIKQLNILVTEQYDHDLYTNHILNIVQHIPDTITTVVITYSHNSNNCNTDKNSDIPPISVETKRIIKLNKSLLDLQYRIQRLLYTLDQIKYLDVPSFTNRRVELKRDTLNPETRLLNWSVDQEIKHYMGKSSIVQITDTTSPEYLVSLFKRYDDYEHKDIHLELHKYYDSQYDQDILDIFKLLVNQIRSVKITVYQIKDEAEQPQETLDASVQRRESLVERVKEMMPAFVKVVEMKGMDCKKTYYNNSTRGVFHINDPKHGAIPATTNHLLWNAFEAPKNIPSGVKRVTLIYVYQTFLGSIFPQTVVSLFLESYNKEIITTALPETITHLGIGKLGVALSMVKLPACLKYLSIGKLTIQFSFEKVRVPATLSHLEINDPDQFTHHWLFSKGALPASVTHLRIGEPKINNKLSTPLLIPSTVQVMDLPRHPENFINYKIVDRQDYYQTTSGVPFTDIASIKHIHIDKPFLSALPSLPSTLESLDLTDNLARFDKNSLPRSLTSLKCVSYRDVPDHARLNYLETKILELDIIKVVFKVSDRSITLSQIPTTKDQFLMNCVLPMEHLSFGSSDLYTLSDILSPDVVIKRLTIASKHKLEIPNSVQYYEAYNLPVNYPASLKRIHLCSRYSFINSGAETAIYPVPSSVKVLIKNQGNPAKYLQFSDKYSKLCLEASKKAGHNNLVSVDSFLVIWRNKFLQAKINDSQRQPRYVTFKAEIVDGVRRLVNKTPIDSSGLLKVKVKSISPIPFGVTFFSIGEHEVKKAVLLALPSSVTKLKVSNYFDIKHDMIPPSVKHLVTTVLQKEIIPPHLEILEIRSRYSKSTLEELKECLPLTLKRVILSVKCMLNEDFLAQDKLDYLMERKLIAPGHCYYQGKTPISPSTTTLIWGLNEIIPVGVIPYGVKKILFCNRFNQMIGQDCIPESVTEIILGAYFTQCLSHLYLPVSLKYLSLGGCNHPIIPHSLPPNIVQIRIRRSFYHYKSLIHLPKSIKYVKFNSYRLKQDHNSNLCSTKVMFKSIYSNPQSEELKVIQDINESIGLYDQFVSTTTTTPTPTLCPPLDVDFHLDVNENLYLVPGLLDHFRVDSMLLDNEFNQCLAKGSLPQTLTKLVFTRPYQFIQETLNLPPNLRYLDFGKVSCSIESLVLPPTLTTVFLPSFVKPVSIPLGFLPHGLKSLHLRADYKNTIEVGVIPDTVEEVSMCQHADMDNLDFIPPSVTKLSLGVYKANILDCVSSSVTDLHIHPVSHNSYSIPANLIPTTITKLSVDQSIKMLNMDEIPNRVKELHLSDCIFTGIIPSTVQHIEIKSYNRELVWGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMQ4</accession>
    <name>A0A8J4PMQ4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">IPT/TIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_008884</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000593">
        <property type="protein sequence ID" value="KAF2069802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMQ4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052014">
        <property type="entry name" value="Dictyostelium_Tiger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31341">
        <property type="entry name" value="IPT/TIG DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31341:SF4">
        <property type="entry name" value="IPT_TIG DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035278629" description="IPT/TIG domain-containing protein" evidence="4">
        <location>
            <begin position="24"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="567"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="5">
        <location>
            <begin position="201"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="5">
        <location>
            <begin position="440"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2069802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="603" mass="67696" checksum="5E4BF0381AF69B7C" modified="2022-05-25" version="1">MYSFLKVNVYILIFIYCVLYSESCPLTKTSPNYMSGNNNEDKREMSIFSGKTDFKYNTGLIIQGKIYARPNSFGIIEYRITNFQDPINNQLNLTFTFPNFSCTYMMQYIYVDSVVQRANIGYLFGRNFNSVKGTWGISVGNRDGGGIQVGEFINDTCASFPISPLIVNYPEIVVYDLDRIINITNITAPVWKPAITDVMFGESTVTIIGFRFLLPTIMVGEYNCTNGFVNDSMIVCSPPRDIYFSNKNQFAVTMTSSKGNGSGSVALGSLLSFIARPYYVNLSGLILNPLRSLLLYGNAAQPSNIQSYNSSQVDFRFSLDAQCGYSYLVNNTFRQSSSLLVCPLPQLLSWTPSALITGGEINVTGHFMHTKMYNSTETGASFYTRYANGTTHPCDTKFYMDPVQSVYYFKCNILPGNDSFTFVGKTITNQTIEQVIRYNPFIESISNTTYNQTGQVTIIGTSFLDSNTTITIGNSSCTDIKVLSNSTRIICTFKSDIDNDYNDHPLPVFISIEDKYNTTSNIFYYNHRERPTMRPIETDTPETPQEILPTTSPASLKENLQKWKTPVIAVCSIVGVAMIIGTGFIIKRYHDNQKVDLKLHLTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNA0</accession>
    <name>A0A8J4PNA0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008576</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000542">
        <property type="protein sequence ID" value="KAF2070102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNA0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35885:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35885">
        <property type="entry name" value="CARBOHYDRATE BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035294469" evidence="1">
        <location>
            <begin position="20"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="321" mass="35601" checksum="EF33E57494D3E811" modified="2022-05-25" version="1">MIKALTIFALLVCLSFTFAQDTTGVVIWNDNNNDTVIGTLDYTNVVQNANAAAKITLSGYTYAGNAHTQMSAYNYNTKQISFVAFDVKNNQEVILTVSTNTWTRVGTILTIQPGNTWAGFQYDQTSNPNNLITTGWNNQGTLFVARLNTITGTYVMMDTIQGDFKGCSFDTTLQQYNLAFVNISGLFVQSYNMAGAIENSVAYKPVNIASRFSFFDGPFSMIYHPKYNINMVTVSLQDSYQPRPTVNVLAYLYSKSGGMSITSMAFKPNYGVTSVVADQNGNGWVYSISNIADQYYFYTFSPYNNAVWKVNSISTPILALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNC1</accession>
    <name>A0A8J4PNC1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007581</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000413">
        <property type="protein sequence ID" value="KAF2071095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNC1"/>
    <dbReference type="OrthoDB" id="20385at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16148:SF14">
        <property type="entry name" value="MYND-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16148">
        <property type="entry name" value="NF-KAPPA-B-REPRESSING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="79"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="273"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="356"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="423" mass="47731" checksum="AA421454E2BCB233" modified="2022-05-25" version="1">MEASEPFVPFLPFTPAPAASDSSLSSSSQSQSSHSSPFNTYTVPSSESSSQHPRDYSGSGSSTENGVCFDWITVHGYTIMDPVLIPLCMWALYIGISRFIKFRKQSNTYFYSITFLTYGIMMTFAMMSDSLSANSNSFWSIAVNYMDVDLTSTVATLFFYCGLADIGKLDMKSIGARLLVCGSSLLIASLWFYSFAITRNDSIWPRILYQGVVGVSCGFYLVFQLYYLSKKLGSFRPIVWLFIAGVTGGIGLVITFESPLWCHYFGSHFNPSYIWFLLSDITMLLISRYIKKSRPQPIVNQLISYYEEDDEHDDPTKTLLSINDIDGGSGGTSRFADLHSQFKYNTFKDDDKDNLKNNNNNNNNNNNQYNYRFMNTAPAPQPYVTVQPQTPLPPIGYVFVSNQNPTRQYFIPTINNNNNNYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP77</accession>
    <name>A0A8J4PP77_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_007753</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000431">
        <property type="protein sequence ID" value="KAF2070929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP77"/>
    <dbReference type="OrthoDB" id="73612at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000004">
        <property type="entry name" value="Transmembrane protein 115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013861">
        <property type="entry name" value="TMEM115/Pdh1/Rbl19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13377">
        <property type="entry name" value="PLACENTAL PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13377:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08551">
        <property type="entry name" value="DUF1751"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01160">
        <property type="entry name" value="DUF1751"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="159"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="290"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2070929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="35854" checksum="C02AE4D8D5954578" modified="2022-05-25" version="1">MESNTSYGGFRIPNFTRLSKVIAGILGFFLFIGLVFPSSAEYLTINAAYIVPPYFFLWTFFTAGLFDTKIISGIINIIILLFSGQYLEPIWGSQEFLKFIAVVNFFSGLFTFIFYLFGYILIGNENFLYRTYVCGFSGIILAFSVALKQLIPEQEFQFFFITLRIKYLASLLVLLNILFSLLGIQSRSLPFVLFGFLISWVYLRFYQKKGGVRGDLNESFSFATFFPDPIQQPVKVLSNIVYQILLKIRLCPVGDSSILPTTTSNSNNNLSYNSADIDRRRALAMKALEQRMQQNQQTTQQPKNDDLPIDDTNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPA0</accession>
    <name>A0A8J4PPA0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 14">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_007757</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 14">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 14">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000432">
        <property type="protein sequence ID" value="KAF2070913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPA0"/>
    <dbReference type="OrthoDB" id="1844at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000032">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000004">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 14" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="15">
        <location>
            <begin position="57"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="16">
        <location>
            <begin position="258"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2070913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="459" mass="50926" checksum="8583EFDEA2C90C56" modified="2022-05-25" version="1">MSSNREIISIHIGQAGVQLGNSCWELYCLEHGIGRDGSIPEQRKQSELNIGGESKDLHTFFNESSNGKKVVPRAIFLDLEPTVIDEIKTGDYRGLFHPEQLITGKEDAANNYARGHYTVGKEQIDICVDKIRKLADQCSGLQGFLVFHSVGGGTGSGFGSLLLQRLAQDYGGKKSKLDFCVYPSPQVSTSVVEPYNSVLSTHSLLEHTDVSFMLDNEAIYNICRKSLDIDKPTYTNLNRLVAQVISSLTSSLRFPGPLNLDINDIQTNLVPFPRLHFVLCSYAPVISKEKAEHETISVDAITNSVFSENNIMAKCDPVLGKYMACCLMYRGNIVPKEAQKAVSHIRTEKSKSVVFVDWSPTGFKCGINSNPPTVVPGGDLSRVEKSVCMLSNTTAIGQVFSRINHKFDLMFLKRAFVHWYVGEGMEEGEFAEAREDLLALEKDYQSVATDEEEPEEAEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPH7</accession>
    <name>A0A8J4PPH7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_009079</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000632">
        <property type="protein sequence ID" value="KAF2069604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPH7"/>
    <dbReference type="OrthoDB" id="1898560at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06466">
        <property type="entry name" value="p23_CS_SGT1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1130.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007051">
        <property type="entry name" value="CHORD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039790">
        <property type="entry name" value="CHRD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46983:SF3">
        <property type="entry name" value="CHPADIPLOID STATE MAINTENANCE PROTEIN CHPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46983">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04968">
        <property type="entry name" value="CHORD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51401">
        <property type="entry name" value="CHORD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CHORD" evidence="6">
        <location>
            <begin position="17"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="CHORD" evidence="6">
        <location>
            <begin position="151"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="CS" evidence="5">
        <location>
            <begin position="222"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="76"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2069604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="323" mass="36536" checksum="3F9C27DE166BF9E0" modified="2022-05-25" version="1">MSTTTDTNNTTATTRKCGNNGCGKEFTEETVDQCTYHPGNPLFRDGLKYWSCCPSKKVIDFEDFLAIAGCSAGRHQVKEVTEKKTAPVSTKDDDKFVPKPIVSKPSADGSNTTKPKSSGPVGSAKHEPIQLPEYVEENDPEDAVITAGTQCTRNGCKAVYKDESSKTEQCVYHYREPVFHEASKWWACCPKYKHAIFEEFLKVKGCKTGRHRFVPSKKADNFVECRHDWYQSFDAIIFTIYAKGVDKDQSKIEFLDNMNISVDLKLANDKVFKKVYQLAGTLNIADSKIDIMKTKVEMKLVKDPQEPWSKLESSELDLYRYQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPT9</accession>
    <name>A0A8J4PPT9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_007368</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000388">
        <property type="protein sequence ID" value="KAF2071318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPT9"/>
    <dbReference type="OrthoDB" id="21537at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057709">
        <property type="entry name" value="DUF7949"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25820">
        <property type="entry name" value="DUF7949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="5">
        <location>
            <begin position="416"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="4">
        <location>
            <begin position="641"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="3">
        <location>
            <begin position="794"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7949" evidence="6">
        <location>
            <begin position="1058"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1011"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1017"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2071318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1116" mass="123516" checksum="B48C4D1372E3D579" modified="2022-05-25" version="1" fragment="single">MRLNTQYLYVFTYILVVFITISYGLTLTNLSSEDYVDMYAKLNNGVWECNTQFILLAEDPTGITSMMPDVSMPVVITSEEMGTSSTKMLMKFGMNLPTTSTTFPLVVKTGDGNTQTTNIDYICEMPPAVLDFEWLHDDSFQYYPAFTQLTPYALLKVKNNNKKIGPPVDITTGLTYSGAVYYLGNDTYYLGVTMQGANAQDYFVDTPITISTYQTPVPYTLKSPFLKYMNTEISKNVNIYPTGTGSIIYYPEAPVIAEYVVAQEVAITTIFWVSYLDNLVFAQGNLTHPTMFKKKYLPWVGGSTANVFNIIDPSTTSTVTFQYQNQFAAPQISGQTNTLTPSNIAIKYTTNSKFQTDVITCMSGQAMAPYPFGFTQGTSTSYLRAFDLNIDYQFASSVIFSDGVYSFNSPTGSLSVDTQPPKPQTIVSYTQIPNTPKTVVRMRITDDLSGFKRTDFGDSSKIISGDLYDGVYEFVVTQSMSLLQVSRGKMNILDQVNNLKSIDVCTDNYVNVDLQRYPFKLLNFSDVTNIRFEKNDINVTNQSVDNNMFIYTNTIDRDLYPGFFVLPFGNVLSFDGLLTYSGSWNDACGCYKIPFTIPKNYPTGLFEYLMVSSQYSQIWETTYFASLLGQDSVLRVYSEQGDLLGPVVTSIDIQNPSVQVPEGQETTITWRLTIEDKYNGFQSGVVSFVSSLDLVRYNFTFGPEQAISGDNKKGVYEFSIKVNGMCKSQPFYIQYAFLQDSAGKFSEYKEGGSTDLTVATSAQFLNPFYTFLDVTTSSAINIDCQNSLTDNVFPVLSSFTFTPEFIDPMYAGPFLNNSRQVVFDFETFDDNGLLLQSLPRVYLIDQNLNIIYQNSKLLSNTSKTATYRCVFDVPYGFGLLSKSGIKVSLYGIVDNQSNFNGYSSTQLADATFKNTIAVEQRLTLNVSIISTNQVFGHGGEITITGKNFEALDQLLINFNNGTISTLPNSIFSSNSIIIFNNSNPVWVSNVTLTIGRNDGSAFSNSLLVQVQNPTPAPQESSSSSTNPQESSSSSHSSSSSPQQSSSDEPPVTNKPQICTNNCGGIDHGECTPSGCVCKSPWVGSDCTSEVIIVKPTINPTSPSTNITVPTTTKDNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ98</accession>
    <name>A0A8J4PQ98_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Carbohydrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008543</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000535">
        <property type="protein sequence ID" value="KAF2070144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ98"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001919">
        <property type="entry name" value="CBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012291">
        <property type="entry name" value="CBM2_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019028">
        <property type="entry name" value="CBM_49"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052879">
        <property type="entry name" value="Dd_Spore_Germination_Stalk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33239:SF19">
        <property type="entry name" value="CARBOHYDRATE BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33239">
        <property type="entry name" value="CELLULOSE-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09478">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00637">
        <property type="entry name" value="CBD_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01063">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="Carbohydrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM2" evidence="1">
        <location>
            <begin position="22"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate binding" evidence="2">
        <location>
            <begin position="22"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="12604" checksum="21100996A311E257" modified="2022-05-25" version="1" fragment="single">GNSYLVTPIVVYVPPDGNNCGLLVQQTIVAQWVNSEIFPFTQVAVTYQNTGTKTITGVSFAMDKVDQYWNVDKVNNRYQLPSWNPTIAAGQYYSFGYIIQSNTVGTLTPTVTCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQE3</accession>
    <name>A0A8J4PQE3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006846</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000334">
        <property type="protein sequence ID" value="KAF2071829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQE3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="215"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="257" mass="28450" checksum="3699463F27571103" modified="2022-05-25" version="1" fragment="single">SSSSSSESEIEKESEGESESEEKKSSSSTSSSDESEKEESKDIVENENNDTSSSDNDDDEENNQDEADSESGSGSENDDNSDDDSESSDSDDSDDDNGKKEKKKNLRGAPSLAHVTKSPSSKKGDIKVNQTWRSLFIKQQQQQQPTTAPVDQQEDNSSSFNLFAIIKANNQKEQTSKKQQTPTATTTTTTTAPKSLFSFNFNSADQSQTEKRKTTTPTTSLLSKSTTQNNNQDEDDEEFWGNKNNKKRKVYNNNKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQK2</accession>
    <name>A0A8J4PQK2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TCP-1-eta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_006890</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000338">
        <property type="protein sequence ID" value="KAF2071783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQK2"/>
    <dbReference type="OrthoDB" id="20459at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2071783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="551" mass="61285" checksum="C8246154C55F3305" modified="2022-05-25" version="1">MKENYEGLDIFKKISSLLESSYGVNGKSKLISLVLDYNQDSSNNDSNNNNVNNDSSNNKVLLSTQKVLLTNDGLTIIRSLSNDSPIYNILLQQCTLIKNQFGVGVSQFVIILNGILQRSMKLMKQYNIHSSLIIDMLRRCKTTLLSSGFDNLTSIKQIDIKNDNILESIIKSNLSGNVLGHHSNTVLKLIKQLMTIIPSLQSQRYSILSIIENDEITLDSSNSSNLNNTSLDSSFVLDGIMYPKTFTFAGSETLSKSISNPKILLLNNQIELKNQNEYSKIEIDQVDQFVEFINSEKKIVLDQLANVYDNDIQMVLNKRMIGDLAIQEFSKHIHKTKRPIQCSGNIPDAQMKSLQIALGGAVQSTLNDLSKESRFYGTCRQYQQILIGKKKYECFSGCGSRYATIVLRGSSSRFLEEAKTGIQDCIKAIQTIVTDKHQVAIGGGAFEIQVSKYLQSSIATHSGKEKIILNNIICESIEYLVQLIGDNSGHNGLHLLKQLQLDPEPKSINIHNGSTQSLDSSNIFEPLSSKLSILNSSLEIASLLLQIDLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQX9</accession>
    <name>A0A8J4PQX9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">RING-type E3 ubiquitin transferase (cysteine targeting)</fullName>
            <ecNumber evidence="17">2.3.2.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Peroxin-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">CYY_006207</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-S-ubiquitinyl-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.36"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pex2/pex10/pex12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.36" evidence="17"/>
    <dbReference type="EMBL" id="AJWJ01000276">
        <property type="protein sequence ID" value="KAF2072475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQX9"/>
    <dbReference type="OrthoDB" id="1701437at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16526">
        <property type="entry name" value="RING-HC_PEX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025654">
        <property type="entry name" value="PEX2/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006845">
        <property type="entry name" value="Pex_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045859">
        <property type="entry name" value="RING-HC_PEX2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48178">
        <property type="entry name" value="PEROXISOME BIOGENESIS FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48178:SF1">
        <property type="entry name" value="PEROXISOME BIOGENESIS FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04757">
        <property type="entry name" value="Pex2_Pex12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="20">
        <location>
            <begin position="384"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="46"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="55"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAF2072475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="447" mass="51196" checksum="242AC4607BF6443A" modified="2022-05-25" version="1">MINSDTNNNSRSNNIYSSNDLMLSSNVSASEINSNNNNVQKIIDNSKVGDNSKQSSTSSPSPSPLQTTTTNTTTTTTTAATSTTPINRNNVPLSSYENSEWNKEYNLEKDKISELQEIIDHSRRPSTKIVRVSQLDSAKLDDEILDLLKSQFMKIFSFFKPHFIERFQPEINLVLKSAIFKLSIFNLGTTYGNQLQNLTYRNEAAFDPHKGSDQLTKLTMRQKWLSGIINVGGEWAWSRINKLSISQGWGERPPNDWRKRLWNILNFLESSYKVLSILNFLAFLYDGKYVTLANRVLCMRLVYAHPALSRRISFEYMNRQLVWHGFTEFVLFIMPLINIDKIKGFIYRAFITRASSNHNQIASSTPQMKQQLYTQQQQMALQKCPICVLEPINIPYSADCGHLFCYYCIKTSIMIDSSFMCPKCNSLVSNIKRFSPLDFTAESIETQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR23</accession>
    <name>A0A8J4PR23_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_006619</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000314">
        <property type="protein sequence ID" value="KAF2072053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR23"/>
    <dbReference type="OrthoDB" id="8119704at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46118">
        <property type="entry name" value="PROTEIN ABHD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46118:SF4">
        <property type="entry name" value="PROTEIN ABHD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="68"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2072053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="322" mass="36781" checksum="D244C4FEFA2913B6" modified="2022-05-25" version="1">MIFRNCSNYNILKSNYTLSGRLAHTIINNNNNGNKLRFYSNSSPIDLVYSVIEPKVVVDKPSHGIKDVIILHGLFGSGSNWRSVSPKIADQTHCNIIQVDQRNHGTSPHSDTFNYPIMVKDLYQLIEKHHKKEDGLCLIGHSMGGRVAMLYALLHPETIKKLVIVDISPSQLKSNTIDEFKSYLERMKSMNLNEIKNRREAEQWLEPVVPDKGVRQFLLTNLVMGDNGKYEWRIYIDGLLDNIHEITFFPAPQNAHFDKATLFIGGGKSKFIRDHDKELIQKYFPNSELKIVPNAGHWVHAEDPHTFVQMVSSFINDNGSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR52</accession>
    <name>A0A8J4PR52_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CMP/dCMP-type deaminase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_006012</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000258">
        <property type="protein sequence ID" value="KAF2072678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR52"/>
    <dbReference type="OrthoDB" id="408702at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01285">
        <property type="entry name" value="nucleoside_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000137">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019028">
        <property type="entry name" value="CBM_49"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052879">
        <property type="entry name" value="Dd_Spore_Germination_Stalk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33239">
        <property type="entry name" value="CELLULOSE-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33239:SF4">
        <property type="entry name" value="CMP_DCMP-TYPE DEAMINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09478">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01063">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="Carbohydrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035147757" description="CMP/dCMP-type deaminase domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="4">
        <location>
            <begin position="62"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2072678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="328" mass="36048" checksum="178F1032D9DDDA9A" modified="2022-05-25" version="1">MNKLGLFLTFLCLFGIALACESDYNPNLVTIGECKANDVAHWRETDSLPVVNPADLPAADRTVHEERMAYILSLARAQNKKFVASIYSPAGELLCVGINTGSPNIISHGEIVAINNCTTLHGIKSFTGHTLYTTGEPCAMCASALLWADFKTIVWGTFNSDLLCKICMSNIPMDSSYIFSRYYGLRPTAPVLIGGVLRADADAWFGTYCNRPTSIYYIKPQCACQNTSSPLNVTQTLVNTWIDGNQVQYSQFNAVIRNNANNVTVTNPTFKSLPSGVNPTQIWGLQKTSVADQWVLSWNPMLTPNQTFSFGYIIQGATELTFNAEAQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRG0</accession>
    <name>A0A8J4PRG0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">IPT/TIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_006741</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000324">
        <property type="protein sequence ID" value="KAF2071947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="20492at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052014">
        <property type="entry name" value="Dictyostelium_Tiger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31341">
        <property type="entry name" value="IPT/TIG DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035191717" description="IPT/TIG domain-containing protein" evidence="4">
        <location>
            <begin position="25"/>
            <end position="1885"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1844"/>
            <end position="1866"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="5">
        <location>
            <begin position="258"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="5">
        <location>
            <begin position="567"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="5">
        <location>
            <begin position="648"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="5">
        <location>
            <begin position="896"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="5">
        <location>
            <begin position="973"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="5">
        <location>
            <begin position="1059"/>
            <end position="1141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2071947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1885" mass="204812" checksum="54E0908A9355F25A" modified="2022-05-25" version="1">MNTLNFYFLIVTLLFLNTLTSTLADPIYFELNGHYYERVIETVDAVNATLNCEKKVWNGKKGYLATYSFEEEWAFSAANNIYTQSAAWITGSDAVKTGAWTYSSGPEKGQPMYNLFFDKCYSFCLFGGVEPNLIPNEHWVHTSGTVATPYWNNNNYLAVIPAYICEYGGLEDPTIPTITTLGGDITITNIVGMDLPSLVITFTNRDTPSTTFKCSNIKVLNSTSVSCTIPTGTGRYRAVFTDSKGSAAKNLLWQYELPFIQSIYPVFKKNEVLTIAGNNFGVSASLVKVLVGTTQTIVCGNVVMLQNHEAITCVLPQDLTEKLLPISVTVNNLRHISYKAGIFYPQNNLYYSGFLTSANWEISTKNYSLELRIDGQNGHLGTMDSPGLWTFLNNSLPLPFNNGIWSMWQGVKWNPSTSKFYYINGPKTGQIANLQYTSTNTDPSTWNDQTRFTQNMYTGLLSSVSATSASGTFTEFGGEDPAFTQVKTHYLDTNGGVADIQIDNYGTVFSKIAVDFRSLSLTYVRDFINSELDVTIPVGYGGPYPISVKVDDKTTAPNTQFIDYNAPTITSLTKVSTRGGETTITGTSFFNDPAVTSVKIGSLICTPVYIVAHTQVKCTLPVGTGILPTIVTVGTRPSGSREFYYIAPTVTSVSTSTPLGSKITIVGDNYGIDKSVISVQVGDFACTGVEITTAHTTITCTVPAGQGSHPVTVTVNYQDSNSDKNLNYNSPTFTNIVQIQDTIEITGTNFGIDLNQLQLLIGTVDITSVCSSSDTKVTCTNLPTTVISGAITTVGAVVNPSQNFLLTPYIISIDPLVIPTSGGSVSIKGRFFETSSMGVSNSLSVVFDGKTDTNNGYSSNQLLVYTVAQNTGVNKVLYIKSSTRTSNSKTISFSPPTITSFSQSNEVITLDGTNFGNDVDQISLSVGSLSISPITLISHSKLSFTAPLSTLNGDIDIIVNTQSFSFSRFTLIPIITNIPLVKVIGESITIQGKYLSNVDQDSQSTNIQFNFSSLVSTCTFISSQSYECQIPSGTGTKAVAYVINDNIRSDDFIYSYQPPTVETITNTIYNVPQQVIITGTNFANQGLGVKIGDQDCNSPLASDDTTITCQFPSTATPPTSNEKLKVQVTVDGQSSSADIFSYASFSVGSVVQVEDFIEISGFNFGDVSKLNIKLGEVVLDSCTGNNTFITCNPLSLSVESGYFDITNGPEPITPIKVLLRPYLYDINPTKIITDGQDITIKGRFFEETSITETTNTILVKTSKNSAGIGYKLVDNTTIIAQETQGSGSLDSISVSVNNRQSNSLDFGYFAPTISSVSHSTTDKRLVILGENLGTVDVVSVYFDSLVLTPTIVESTRLMVSTPETIKNNDVYIKVSDQTSNTLKVRLTPGITTVGPSPPTNGSTISLSGRFWNTPSITFYYSLLQESSPSYKPLTCKPISFESIVHTTECTLPSGYGNFSVQAIADTIVSNEFALDYQSPIVISATSLLYKSPGNVTINAQNIAHPSTVTIGGKECTNAKLLDSEHIQCFYDGSVAANDKQDPLKITVTSYHLSGSNDVFLYLAEKGCPGTPECSGNGVCNRETGVCKCFNETVTTNDCSVVDENVLPPITDDNGETTIPGLKFNFTIAITHLREINLQEQSVKVLKVSTIKWNDRYKLSDTTSYYKGGFDNDPAVLELKVTYFDSLSNIDFAGDILSMPANSIKYEISVSKWSFDNSFNYLQVIYNSKAEKTTIYNCIEVPTNITKLNVDSLSWFQVNAGGSTLNAKFSDRIIVDKRITKSSVRLLDNTDPLYQDVSADPTKFNVLTALSTPYFTEKVVIDPNFSALLRSETTTACSTKQKWKIPVIVALCSAGGVAIATGAAIFYKKKISMKKLEKSISMQSRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRG1</accession>
    <name>A0A8J4PRG1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Serine hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006987</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000348">
        <property type="protein sequence ID" value="KAF2071706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRG1"/>
    <dbReference type="OrthoDB" id="414698at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005645">
        <property type="entry name" value="FSH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050593">
        <property type="entry name" value="LovG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48070">
        <property type="entry name" value="ESTERASE OVCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48070:SF6">
        <property type="entry name" value="ESTERASE OVCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03959">
        <property type="entry name" value="FSH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine hydrolase" evidence="2">
        <location>
            <begin position="14"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="266" mass="30087" checksum="48D9F898F6831607" modified="2022-05-25" version="1">MDNNHSSESTTPTKKLRILCLHGYKQNGTIFKSKTAVLRKSMKDIADFVYIDAPHIVDESKGTASWWRASKDGKEYRGWETTIDYLRKVFINQGPFDGILGFSQGAVLSGLMCSISSLINDSDHHQNNNYHNHHHHNNNNNNNNMPLNFDECFSLKFGLLFSGFQSRATIHQSLYPASSNESSGSCESSCSSNPNYPLAKITTPTLHVWGKADELVSATNCECLSHHFSSPNCYVHEHGHLIPTSKIDIQNYRDFLQRFLRDCELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRH5</accession>
    <name>A0A8J4PRH5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RWP-RK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_006034</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000260">
        <property type="protein sequence ID" value="KAF2072655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRH5"/>
    <dbReference type="OrthoDB" id="30748at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003035">
        <property type="entry name" value="RWP-RK_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51519">
        <property type="entry name" value="RWP_RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RWP-RK" evidence="6">
        <location>
            <begin position="264"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="28"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="163"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="45"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="178"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2072655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="375" mass="41179" checksum="603DBC930EB2C314" modified="2022-05-25" version="1">MIYGHIPSAEFFRPIPMSSQSHILNNIIHTDPVGSGESHGNMQDNSNNNNNSGLSNLNSINNMNNGLNTLSSLSHLGSPPNNNNNNMGNNNNNIGNNNNNNNNTPNLPSSNTNPHYSSPYQKPLSLTKDRIPVHLQHYMNSSSSMGGQNFERDNIEEIREVTPTNITLSRNIGSGPASPTSSSPSSSSPSSLPNPTSTSNNNNNNNNNNNNQNSRKNNNSNNSNNSHNNSNNNNSNNNNNNQNDSSDYEGGNDNQSDDGDRQPNMSNAIVPAEDRSIVKTNQYIEITEYLNFPQSEAAKRLGMPTSTLSKRWKEAARGRKWPFRKVSKIDKEIMTLLHNIPPGGEQASLPTAVDSALVALLKKRQKKLRPVFIRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRH7</accession>
    <name>A0A8J4PRH7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_005729</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000236">
        <property type="protein sequence ID" value="KAF2072963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRH7"/>
    <dbReference type="OrthoDB" id="19656at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001401">
        <property type="term" value="C:SAM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051654">
        <property type="term" value="P:establishment of mitochondrion localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070096">
        <property type="term" value="P:mitochondrial outer membrane translocase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="entry name" value="Porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027539">
        <property type="entry name" value="Mdm10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28035">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28035:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12519">
        <property type="entry name" value="MDM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1134">Transmembrane beta strand</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2072963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="292" mass="32246" checksum="F77F12CEED8E8290" modified="2022-05-25" version="1">MDELSHYPGSYFDIDYEANDRIDFQDDGDQGLNVTIDKSYKDNHINIHTSPFAVNKFSISNTAVEGQVFEASISRDQNENVLELSHVGELLKGVTSHVFVSVPQKELKQTDGVATLTLRNHRTNFELKGSTQNNGLLGFSLLHGVKSNLAVGFELYYKLGQNSGGASVATRYRGNLINKNHQYQLTSTYNIMGDLSASISLGIIPQCLSLSTRYNLNTSSMTSTLQIGTQILATPKIMDKAIPICLKLKTDTELNHAVRLEFEAPLFSVSLGSYSNKQSKNFLSNYGIGIHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSG4</accession>
    <name>A0A8J4PSG4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA damage-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_006089</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000266">
        <property type="protein sequence ID" value="KAF2072592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="20774at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018846">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10433">
        <property type="entry name" value="Beta-prop_RSE1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 first beta-propeller" evidence="5">
        <location>
            <begin position="56"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="4">
        <location>
            <begin position="1121"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="128"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="11"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2072592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1284" mass="145605" checksum="860296505D05C60D" modified="2022-05-25" version="1">MLSSQNTHQANLLPPSVVNNSNNINNLNNNNNNNNNNNQLNKGLLYAKTLLPPSDITKVVKGNFRNNSRSNDSVQVVYIKDRYIELVEFFYDSESFESIDQSPLFSTVKDIKVLSPIYYNNLSTPAATNTTTTTTSHSKYSNLENNNNNNNNNKSLATSTPIPISSFDLDFRKKDLLAITSDSGYLSILSWSNIKQQFLAVQYISISPPGYSHKHLGEKISISPCNRFILVSAIRNKISIFPIINDLNDPLNVVSTNKVDIEIKGIVESCGFLYNEKDVNLNSNEIYITVAFRNENSANTNLDQHSGSLQGVIDIYKYDIKSQLITLTEQKKIPFSGQKIITFPSPKINNNSFNINSYFLVLLNSTQLWLMDKHLNTLQEYPLILDKTFTLQEKSTKQDFSLITSTCWMWNSTIEEQETSMTENEHNLILSDDRGFLYRVLIQLKDRKLVLVHSISDQINSKRILPVSKTLSLGKGFIGLFRNLSDCELFKIVENSNNNNHIKTTIISRSGITDFLMIPTQFSNQIYCCCLGSNTMGSLKLIESSLPISISVKDDALLTTGVWCFSTFTRPDIKYSVVASVSSTMVIKFKDNVGTLFTDQVGFISNQETLYSSCTNQGSLVQVTRKSIIVSFELNSNNNNNNVIEYQRMIEWNCPENSIIVSCCSRDNILMVSLSQPNTLLYFMINNETKKLDLVQSQLIEKEISCVCIPSMDHINNNPLNSIESICLIGTHEKEVLVLNFKTMNIIYTFGSIPSIPHSIILNSLGNNQSLKLLVGCRKGELIKWDFNLQFLNNNNSNNNNNNNNNNNNNNNILTNQYTKKISNSQLLLISNLDQGALILTDSAYYVTSIKSHLNILKISSPERISSASTFNSLHSKTDFIIITPTSINIITLDFSQLFSVTDLTKKLNEKDNSNNNNNRQNPKKIIYLKTFDLIGILYDNGFTLYEPSKGIDPPKSILPIDLPKKQWHGFKYWDERESIIVYSDRCEKDGVIYIFNIKYDPDGNDIRVLDKKQFQFHRPILSVSFYGKNQLYVFTTNEKKIIYLGDHQKESEDLSSLINNNNPIILSKFNNDRLILGFQSKGIEIYQKNPANNNRLEKKRSQNIVHLEDSIILNGHGGGDQIASIDVYGNFSIVDFNDIQLDSNGEEPTFKSLDPIVQFSLKEACEKLLELPDGSILTAGNLGSIFLLIKVPSNRHFNILHSLQTSMLKYYYTRPLLNNNHSVFRSEVGPQKGVLDGDVISQFLFLDENEQLELLSTLNLDYKIEPNEIISLIEYLNDPKYIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT18</accession>
    <name>A0A8J4PT18_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">CYY_005944</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Nucleus</location>
            <location evidence="16">Nuclear body</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000251">
        <property type="protein sequence ID" value="KAF2072756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT18"/>
    <dbReference type="OrthoDB" id="5600418at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037381">
        <property type="entry name" value="RFWD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056527">
        <property type="entry name" value="WD40_RFWD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16047:SF7">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RFWD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16047">
        <property type="entry name" value="RFWD3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23419">
        <property type="entry name" value="WD40_RFWD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0853">WD repeat</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="20">
        <location>
            <begin position="264"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="179"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="340"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="21">
        <location>
            <position position="807"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAF2072756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="807" mass="89810" checksum="B512413FEABCA2C2" modified="2022-05-25" version="1" fragment="single">HNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNDNDGNRSNDNNVQDNPINQGMNRMNYYPNPFSINNSVFDEDEDDSDCSCYEDDNYDLIDERMVSIQISRKILRNQLIIKKKEDEIKRDIEMAKSQSIPLDLDALKNTQHANESDMKSHNIFDRVQIFNSISTLEKPAIPFSPNKPTTTTSTTTTAAPTFSRPTLGNNFPSNKFTEHYNSSKNNEQPTNATTATTNNTNSTCEFCDPSKPLKRIDIAEEGNGCPVCLDVFHSTGAQRPVSLKCGHIFCKECADKFISELKKCAICFTKAKRNDPLPIFLPNGATTVDPNADALRCRAVIKSRDREIESLKQKVLNLKARLSQSQTLNAQQIQSSISRPFATTTYAAYTNNKKNNNNIVDAVPTVTQNQSKPLVLPLSRSAKAALERATTTTTTTTTTTTATTNPQASMFKNNAEIPDNSTNFQPLSLYKLNASKTFDFSCESGIIVASYSKNQRDYGLIKLSSLDPSNADFIPNLHQGIIRNVKFSNDSRIACTVSSDRQVKLVNINSKSVICTYNLQTPGYSCQFDHNNQFLFYASEIGKIHTFDIRNTTKPSTTIIVEGKNSESPIFNIAYEKAKSQIQCSPLSGESVGHGGLFASFNDLGYYEKNTSQPEKGQVYATRYQVPLFGQCISVANDPVSGYCLASFRSSPTYPKNSHVVFKHLGRGAIEEIRTIYTNIVQTVPCPSILFSSYYNSNCYVASSFNSETLGKTGDDGVNIWNSNTGQLSQKINLKSPLSDLLHIKYQAEDYLLSLSASELNIFKNNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT86</accession>
    <name>A0A8J4PT86_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="8">PPIase</shortName>
            <ecNumber evidence="8">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_009700</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the cyclophilin-type PPIase family. PPIL4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="8"/>
    <dbReference type="EMBL" id="AJWJ01000782">
        <property type="protein sequence ID" value="KAF2068981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT86"/>
    <dbReference type="OrthoDB" id="2083at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01921">
        <property type="entry name" value="cyclophilin_RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12235">
        <property type="entry name" value="RRM_PPIL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000015">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035542">
        <property type="entry name" value="CRIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035538">
        <property type="entry name" value="Cyclophilin_PPIL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45843">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45843:SF1">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="10">
        <location>
            <begin position="6"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="240"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="339"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="339"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="376"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="456"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="487"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2068981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="499" mass="58562" checksum="D3A89C3F4819968D" modified="2022-05-25" version="1">MSVLIETTLGDIVIDLFTKECPSTTKNFLKLCKVKYYNGCLFYNVEKDFLVETGDPTNTGKSGQSIYGLLYGEEANHFQDEIKPSLRHTRIGTVAMANKGKNLNDSRFYITTKENIDYLNDKHTVFGQVEEGLDVLEKINSFFVDSSNKPLQNIRILHTIILDDPFPDPQGLEIPNQSPRFKKSEQDTLFEADEDLDKENKNKSKEEITEMIDEKAARSRSELLEMLGVLPTADIKPPENVLFVCKLNPATESEDLEIYFSQCGKVKSCEVIRDAQTNESLCYAFIEYSTKEECERAYLKMDNILFDERRIHVDFCQSVAKVKGRGDHFKNFLNRGKSNLRSTSTQHIPSGTKGYDFVFDDDHNRDHSSSSSSKKIKYDNEKKSDRDRRDDRDHRDYKKDDRYSNRDYKRDDRDRRDDRDDKHYSSRRNDDRDDRNYSSSNRDRRDDRNYSSNSSRDYRDKRDDRDDRNYSSSRRDDKNYNSSSSSRDYRDKRDDRDKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTL5</accession>
    <name>A0A8J4PTL5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ATP synthase subunit beta</fullName>
            <ecNumber evidence="12">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_005252</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="12"/>
    <dbReference type="EMBL" id="AJWJ01000204">
        <property type="protein sequence ID" value="KAF2073435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTL5"/>
    <dbReference type="OrthoDB" id="14523at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042776">
        <property type="term" value="P:proton motive force-driven mitochondrial ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18110">
        <property type="entry name" value="ATP-synt_F1_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18115">
        <property type="entry name" value="ATP-synt_F1_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01133">
        <property type="entry name" value="F1-ATPase_beta_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1140.10:FF:000001">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000026">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="entry name" value="Bovine Mitochondrial F1-atpase, Atp Synthase Beta Chain, Chain D, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01347">
        <property type="entry name" value="ATP_synth_beta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005722">
        <property type="entry name" value="ATP_synth_F1_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050053">
        <property type="entry name" value="ATPase_alpha/beta_chains"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01039">
        <property type="entry name" value="atpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184">
        <property type="entry name" value="ATP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184:SF71">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 12" id="KW-0139">CF(1)</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="247"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="621"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2073435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="651" mass="70983" checksum="1A2B0E5E4D051C66" modified="2022-05-25" version="1">MFVSRRLGSKVAQIRSVSKVAAAVAPKRAFSTQSQEKGKQTQTKSANPNINEFFDKNVDRLDPTKNGIAEKKVDLAENQGLVHQVIGAVVDVQFPHGKLPHINDALIVEDFQTENVEKVIEDLNNPELQGKIDFTQVPISKIKLVLEVAQHLGEGVVRCVALDITDGLQRGAKVINTGAPVMVPVGNGTLGRIINVVGDPIDQRGPIDTKEFRPIWREPPRFDELAPSASILETGIKVIDLLAPYSRGGKIGLFGGAGVGKTVLIQELINNVAKAHGGFSVFTGVGERTREGNDLYHEMVDAGVIKLDGTGSKVALVFGQMNEPPGARARVTLTGLTLAEYFRDAQGQDVLLFIDNIFRFTQAGSEMSALLGRIPSAVGYQPTLATDMGSMQERIATTKNGSITSVQAVYVPADDLTDPAPATTFAHLDATTVLSRAISELGIYPAVDPLDSTSLMLDPNIIGEEHYNVATSVQKILQEYKSLQDIIAILGMDDLSEDQKSTVFRARKIQRFLSQPFEVAEAFTNMQGKFVKLSDSIQAFKGILEGKYDHLPEAAFYMVGGIEDVEKKAASLLESTTETKKTTTNAAGETKTESLREKVKRLVDEAYFAKIEKLKKYGVSTAELEQMRAEYEKNFEKEISEIETLMAKGDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTN7</accession>
    <name>A0A8J4PTN7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005529</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000222">
        <property type="protein sequence ID" value="KAF2073176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTN7"/>
    <dbReference type="OrthoDB" id="17025at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040283">
        <property type="entry name" value="DDB_G0292058-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31414">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN DDB_G0292058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31414:SF18">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035325198" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="515"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="553" mass="60799" checksum="D816D85E0F084F14" modified="2022-05-25" version="1">MMKYILLVSFCLFVLLSSTSSNSFVNGLDADNEKKALDILDAWKDIPRYTYFTSGRSLTKYPAGFCGGTNVEDCDWAKYAEAVVLLSGVTFIIALVTLVFGIIFWLLRSCVFGGCRPTHGCFCPGPKYDPDIGEGYTTFKVWILKIVALIFVIGYVVMFIIALTGNSKSTNGVSDLSDAVFDKVTVTLDKLYEINATLEDPKYRDFEQFQDQIESELNSIIVEGENFRDNGTNVADQVKKYSNMRQSIVLAGLIVALVICAVLVFAGLCNVPVFSLIAALALVIMIPYMWIVFSAHYPINSVLSDVCLSFNSTGFNQLNNFSNPIISEVFDGCEQDPNSTMTVFTDLENLVNSLIDQGYNVTCGKLEQACDLEYPYFANPNLPPVNRTVLSGCPSSCTRDNINYYFNNVTVTDFKYGCRINATYSTCGGDISGCAQQNLITCDYKEVPSVNSCITQCTNQELVTVSNVSITYYNTLTELINQVWIGLVTPLIKCSYLLPFVDEVQGIICVDEVNALTLLIGPTGAAACLLTGLGIMAVLGTKRFNKKAHVKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTY4</accession>
    <name>A0A8J4PTY4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nuclear pore protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_005625</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the nucleoporin interacting component (NIC) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000229">
        <property type="protein sequence ID" value="KAF2073070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTY4"/>
    <dbReference type="OrthoDB" id="1918363at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007231">
        <property type="entry name" value="Nucleoporin_int_Nup93/Nic96"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11225:SF4">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11225">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP93 NUCLEOPORIN NUP93 DEAD EYE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04097">
        <property type="entry name" value="Nic96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="4" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2073070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="900" mass="103417" checksum="C5658DDA30DBBCEB" modified="2022-05-25" version="1">MNGRENIDFNDLTNKSNQIINRISSFSSPIGLSSFNIERSLQEIGETSKHLSDVTSSKAPYSTDTSVDNYPKAIKKYGLDPKLLSKNLSNIDTSSFTDIETYPTDIDGYLKIEFENMILNTINDIQKKTCKEFQSSYENSLVRDWNKDKKYIEEILGQKFIKTASASTGMMGTASRYGSNTSAADFATSKMMNRSVAPTNFGQSQFGRSSNIFNQSNRMQSMDLHNQQQQQYQVGGQDRTKLSNKMRKYAMVIEEFDTHRADVSRVNRFPIVHRLMQANLSETTVNHNVVNDVWNLVIRLTGEKDVVNVSDQNDLAVPQASVKPSGAELITRSIKFLEEQFNQVIKHRLPKISNISSPLDEMVAYISANIPTPPIDHPDEEYKGCSVWSIIYFLLRAGHYQVANDFIVKNSTAAGRYKDLLSNAIQMRCRNERIKKEIKDKLIIEYNRIKSESRDSFKMGVFNFFSNSESNSTKMLFSSIQDFIWWKLNFIKESATAIEINQNSLDTLQTEINNTLSKNNFEVFDVFQILLVTQQFENAIVYLHQTNPEDALHLAISLDYYKLLNIMSSSTFNYQASTSSLSNSRSNPTIAASENLISSSLEIYNPSTKSIHLVAMIRQYTKHFSSETDNKEALLYYLLIENENLKTHLISDLVVTSTNGFEFAKQLQYYTTFINKQQWIRIIEQSAIELENKNDYQGAIGFWLLIEEYARVLEIYNNRISVLLTGSSKEREQLFKFGIQLFNERQMQLKTTRTERTSYLAFEQLLQLCQFFDYFNQEKYQEALLEIEKLNILPLSEHDIDAKVDIYRFLSSHITKNFAAILQAYIDIITKQYQLVYNNSNINTNANNANNNYLYAGRLQTINDLQIRAQAITLFAGKIDFPMSGNILTRLMNFVISIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU53</accession>
    <name>A0A8J4PU53_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GrpB family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003841</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000126">
        <property type="protein sequence ID" value="KAF2074853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU53"/>
    <dbReference type="OrthoDB" id="630895at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007344">
        <property type="entry name" value="GrpB/CoaE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34822">
        <property type="entry name" value="GRPB DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G01530)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34822:SF1">
        <property type="entry name" value="GRPB FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04229">
        <property type="entry name" value="GrpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="170" mass="20073" checksum="9A92DE555181940F" modified="2022-05-25" version="1">MKITIVDYDPNWVHLFEKESKVIKQCVGQDNFINCIHIGSTSVPRLKAKPIIDMVLIVNDIYELEKSNEEFIKLGYEPMGENGLIGRRFFRKAFDTHIHAYQFDNVKEIQRHVSFKDYLIDHPHVCAEYADLKQRLAREDRDMAGYCEGKDAFIKHHEAEALRYLWRSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU73</accession>
    <name>A0A8J4PU73_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">O-methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_005045</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000191">
        <property type="protein sequence ID" value="KAF2073660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU73"/>
    <dbReference type="OrthoDB" id="1606438at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712:SF2">
        <property type="entry name" value="O-METHYLTRANSFERASE CICE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_4G14580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005739">
        <property type="entry name" value="O-mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="6">
        <location>
            <begin position="39"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="5">
        <location>
            <begin position="148"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR005739-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2073660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="395" mass="45602" checksum="024F15AD1647F717" modified="2022-05-25" version="1">MSTKESLPPIIGVYIMRCIYSITYYWEKIVPQIKFHRITHDFIVFQCLSVVFTLKVIDHLKESPKNVKELAVLTNSSEKNLFRVMRALSSEGFFSFDENSGKFSINSMSTLLTNNQITKENPTGINSDEESLSCLFSMIQYPAFIEAWNSLKDCVQTGVPGFHIKHGMSFFQYIDEKDTYLKKIFDSAMKQKNFMTKIHTQISQNYNFSDYSVICDIGGGVGYLGYEIISRHPKCQIHILEIEETVKNGLELCKGDSKKMLAIENEKMIFKVGDMFQPKTIPRANVYILMQVIHDWNNQDALRILSSITAVMRREKAITHQRPKLLIIDYIIEDNFNQDSYKKISISDIIMMAIVGGEERTRSQWNFLFKETGLSLLSVKKFNRPPFLSLMELTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUG4</accession>
    <name>A0A8J4PUG4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004556</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000163">
        <property type="protein sequence ID" value="KAF2074153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUG4"/>
    <dbReference type="OrthoDB" id="5779169at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1310.20">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="1">
        <location>
            <begin position="36"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="227" mass="25165" checksum="93D761F0879B4A7C" modified="2022-05-25" version="1">MKTITKILTTTKRIFKDRESAGNELATYIGNELKGQPLDNAIVLGLPRGGVPTAFCVAEKLSCPLDIVVPRKIGAPGHEEYAIGAIDENGQGYLNQSVIEMMGISQRYLHQQIELEKKESQRRRSVYRSGKSPIELKDKLVILVDDGIATGSTMKAAIESVYSKSPREIIVAVPVAPDDTLQDIQEMKKVDKIFCLCVPSNFQAVGCFYKVFDQTSDETVIRLMNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUM6</accession>
    <name>A0A8J4PUM6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mediator of RNA polymerase II transcription subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_005223</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the Mediator complex subunit 7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000202">
        <property type="protein sequence ID" value="KAF2073475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUM6"/>
    <dbReference type="OrthoDB" id="10253553at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037212">
        <property type="entry name" value="Med7/Med21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009244">
        <property type="entry name" value="Mediatior_Med7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044888">
        <property type="entry name" value="Mediatior_Med7_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21428">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21428:SF11">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05983">
        <property type="entry name" value="Med7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140718">
        <property type="entry name" value="Mediator hinge subcomplex-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="204"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="216"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2073475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="258" mass="29294" checksum="117CF2DB524E7A8A" modified="2022-05-25" version="1">MNAAASPQQIENVSTFPPPPPFYKLYLNYDSDIDSTATTSATTDPSTSSTLSEQQSIPNIILPLQPPIPPKQGSNYNQFGQTYSTVDVLPTLDELDMVQLYPKGDIEPISELKKLNRSILFNYLQLLETLIENPSDFKTKVDDISLLFINFHHLLNSYRPHQARETLLSIMTEQIKNRTQSNQLLKKSLETCKESLIQTFNNLNNETEEKEKLSPTLATTTKEQPSTPQTINKNEELVKPTNWLDQLVNELSKIEKPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUW3</accession>
    <name>A0A8J4PUW3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein archease-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_004256</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000147">
        <property type="protein sequence ID" value="KAF2074438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUW3"/>
    <dbReference type="OrthoDB" id="2190767at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072669">
        <property type="term" value="C:tRNA-splicing ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.55.10.10:FF:000002">
        <property type="entry name" value="Archease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.10.10">
        <property type="entry name" value="Archease domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002804">
        <property type="entry name" value="Archease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023572">
        <property type="entry name" value="Archease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036820">
        <property type="entry name" value="Archease_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12682">
        <property type="entry name" value="ARCHEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12682:SF11">
        <property type="entry name" value="PROTEIN ARCHEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01951">
        <property type="entry name" value="Archease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69819">
        <property type="entry name" value="MTH1598-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Archease" evidence="6">
        <location>
            <begin position="10"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00071898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2074438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="148" mass="17063" checksum="B61FF0D2843E0F7D" modified="2022-05-25" version="1">MGNLGQLCNFEYLDHTADIMFVWGKDLKESFEQMALAMMHYMVELDTVEMDDSAVTHEIKVSGHDMDSLLFAYMDECLFIFNTEFSIFKEIEILSIDRESFTIEARAKGIELDKSKHTTGTEIKAITYSCMEIKEAQDKSEVYLIVDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVD8</accession>
    <name>A0A8J4PVD8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005862</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000245">
        <property type="protein sequence ID" value="KAF2072819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVD8"/>
    <dbReference type="OrthoDB" id="16464at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:001523">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050177">
        <property type="entry name" value="Lipid_A_modif_metabolic_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245">
        <property type="entry name" value="BIFUNCTIONAL POLYMYXIN RESISTANCE PROTEIN ARNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245:SF11">
        <property type="entry name" value="LD23561P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="367" mass="40725" checksum="15CC3A5089ACED24" modified="2022-05-25" version="1">MSKPNVLILGGVGFIGRNLVQYLVEQQVCNKIRVVDKVLPPTAYLGAKHQEAFNNPSVEFMQGNLTSPASIAKVFALEGDKFNIVFNLAGETKYGQTEAVYNEKVFDVSVKCATEAAKVGVDKFIEVSTAQVYESNKKPSKETDKTTSSKAWTLIAVHKLKGEEKLKEIAGLNLIIVRPSVVYGPGDILGISPRIITGAVYQFIDEKMKFLWTGDLKYNTVHVSDVCKALWHLAKVGKNGDLYNLSDKGDTDAEKISKILEKIFHIKTSFVGSLLSNVAQLNLQSICNEANDKHLKPWSDLCKQKDIVNTPLTPYIDQELLSNNHLSVDGSAIESTGFKYDHPELTEALVRDQIQYFIDQKLFPALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVQ0</accession>
    <name>A0A8J4PVQ0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000483">
        <property type="protein sequence ID" value="KAF2070496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVQ0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="451" mass="53354" checksum="28E505C42966AAE9" modified="2022-05-25" version="1" fragment="single">MKKSITTFINDKIKRILLLHNHDLYHDINQTLKDLFSLKRYIIRNNENNNNNSIWSMDDEYRHHLYSSDEFLNNRNEFIFKDTLTFDFGNILTVINEIQTLATKTTKIINNDDKDFYQQQQQLFNLVLILNVNYNNQFDNCLISMIEELKDLIHSIKFQIYQKLTIETLIYNTKLSINSLVIDSINNKCLKNLLKRIENIIFATTTTTTTTTTTTIKEDKCQIYDYKDKIDFDKYNFQLSNIPIPNPIRGIIPKGVIGVHYQRIENNFPIRPIPESVIYIKFDEFNKPLSEIKFPSNLKFLSLGRFSQFIYRNIDIPSSVTHLENDQHGNTIDNHMDHYAYHWLFCKGTLPRNVTHLKIIDPPMTHTISTPILIPSSVKYLDFNNNNNIGYYNFQIIDQSEYYKESITKDSFNNLNTLKYVKFPRLELMPDGKTRIPFPERKIRSEIRPTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVV8</accession>
    <name>A0A8J4PVV8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002734</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000078">
        <property type="protein sequence ID" value="KAF2075978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVV8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043007">
        <property type="term" value="P:maintenance of rDNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000042">
        <property type="term" value="P:negative regulation of double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033045">
        <property type="term" value="P:regulation of sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032245">
        <property type="entry name" value="RMI2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33962:SF1">
        <property type="entry name" value="RECQ-MEDIATED GENOME INSTABILITY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33962">
        <property type="entry name" value="RECQ-MEDIATED GENOME INSTABILITY PROTEIN 2 RMI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="273" mass="31551" checksum="13F99B82FF5B292E" modified="2022-05-25" version="1">MSQYVKLFLSDLCDIQIKPCFKWGTLDKIESIHVEGVVVDCNNKHITLDDGTSAYSFNRSTVYEKDISLNQYILLGLELDAIGSEIIFIQKLFINLSNQVNRKEIWNLEVLHARRYLYNMSLPLNNNSSKSKATTTTTTTTTTSTFTARKSTIPSKTNFNLTTSDLFKKNDPIEFTDVFNNSNNSSHSLTGSEKDEMQTFDYDFSGISDFNNNDDSNNIMLGKDIYNQEEEEENIPVYEEIEEEEEQEEQEEEQMNVSNNKLNFDDEWDEDFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW24</accession>
    <name>A0A8J4PW24_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_004898</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tyrosyl-DNA phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000182">
        <property type="protein sequence ID" value="KAF2073804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW24"/>
    <dbReference type="OrthoDB" id="20291at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017005">
        <property type="term" value="F:3'-tyrosyl-DNA phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09122">
        <property type="entry name" value="PLDc_Tdp1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09123">
        <property type="entry name" value="PLDc_Tdp1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010347">
        <property type="entry name" value="Tdp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12415">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12415:SF0">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06087">
        <property type="entry name" value="Tyr-DNA_phospho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02809">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="21"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="112"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="136"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="313"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="520"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="522"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="11">
        <location>
            <position position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR610347-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR610347-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR610347-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2073804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="613" mass="69524" checksum="6B5BA14AD7D17C84" modified="2022-05-25" version="1">MSSVNDDDRLTPSARAALKRFNTFQEQNSGQQRVDDKIEGVQNQQPAKKLKSVSDDNAYVNQKQQTQIQNDIIVLDDEDTEPEDNTSQKIISIITNDDEDTEPEDNSKKSNKSNNTNSTNSNTLKPTTSVVKKLKLPPLTLKKPTSNSTSTHNQSNSGSSNSNSNSNNKKNIDPIDIDLDDEDEDLRYAIKLSLEQSNNSKQTTSTSTTTTNETLSSSTFYLNTIKNQPRIVPTKTLSIQELFANKGMKSAIITSYVFDVNWILENLYKSQGKRIPITFIKHYDSKVDKKGIFKQSETITVVHPPLMPFGCFHAKLLLLTYDDYIRVALSSANAWEHDYAVLGQTIWYQDFPKSSSSTTADPKSFGSTLKRFIKSTGLPIDFLNQFDFSDAKAVLVTSVPGYYKMTDYEGILQIKHHVTQMNAPATAPTTKDKDIYFQASSIGHNNSNWRGVFTDTLTPKTTGSRNLHIMFPTHQWIHSDATRYQNSGCMFLTEKNYQNNKSNFDVLVPRYLYRKELVLHSKIMVATSKTNDNHLRYDWVYTGSHNFSPSAWGAFQKNNTQLYISNYEIGVLILNDKNQNDKSTPEWVQHIPFQIPTTRYSAKDEPYIQSKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWI1</accession>
    <name>A0A8J4PWI1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Aminomethyltransferase</fullName>
            <ecNumber evidence="4 12">2.1.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 12">Glycine cleavage system T protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_004232</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">The glycine cleavage system catalyzes the degradation of glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[protein] + (6S)-5,6,7,8-tetrahydrofolate = N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16945"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:10492"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="2.1.2.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 12">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the GcvT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.10" evidence="4 12"/>
    <dbReference type="EMBL" id="AJWJ01000146">
        <property type="protein sequence ID" value="KAF2074450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWI1"/>
    <dbReference type="OrthoDB" id="10263536at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004047">
        <property type="term" value="F:aminomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006546">
        <property type="term" value="P:glycine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1400:FF:000001">
        <property type="entry name" value="Aminomethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1250.10:FF:000002">
        <property type="entry name" value="Aminomethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.110">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1400">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1250.10">
        <property type="entry name" value="Aminomethyltransferase fragment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006223">
        <property type="entry name" value="GcvT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028896">
        <property type="entry name" value="GcvT/YgfZ/DmdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029043">
        <property type="entry name" value="GcvT/YgfZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013977">
        <property type="entry name" value="GcvT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006222">
        <property type="entry name" value="GCVT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00528">
        <property type="entry name" value="gcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001567">
        <property type="entry name" value="PRK00389.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757">
        <property type="entry name" value="AMINOMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757:SF2">
        <property type="entry name" value="AMINOMETHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01571">
        <property type="entry name" value="GCV_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08669">
        <property type="entry name" value="GCV_T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006487">
        <property type="entry name" value="GcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101790">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103025">
        <property type="entry name" value="Folate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="GCVT N-terminal" evidence="13">
        <location>
            <begin position="35"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Aminomethyltransferase C-terminal" evidence="14">
        <location>
            <begin position="318"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR006487-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2074450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="403" mass="43829" checksum="B4EC55E023E6DD45" modified="2022-05-25" version="1">MNSPKLLIKYLQKAQGGSRCCFTTTTEVLKKTSLNQLHKDLGGKMVPYCGWEMPVMYPMGVLKEHIHCRTQSALFDVSHMGQLRIHGKDRVKFFESIVVGDLQSLAVNHSKLSVFTNDKGGIIDDTMITNAGDSLYVVVNAGCADKDIAHITQKMKEFGGDVSMELLGDWSLIAVQGPATEQLLLSMVKSNVDLTKMEFMTQHPMAINGIDCIVTRCGYTGEDGFEIAVKSSHAVKLAESLLATGANSADKGIKPAGLGARDSLRLEAGLCLYGHDLNEDITPIEASLNWLISKRRREEGGFPGADIIQKQLKEGVTKKRVGIVSTGAPAREFTDILDPNTKAKIGSVTSGTVSPMTKQSISMAYVSTPLSKNNAPVILSIRNKEVQATVSKMPFVPHNYKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWM7</accession>
    <name>A0A8J4PWM7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005413</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000215">
        <property type="protein sequence ID" value="KAF2073284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWM7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="701" mass="82428" checksum="64836E92BCC86133" modified="2022-05-25" version="1">MNKEYYTAVFDNKYLSKKIFSIIHEIQKDRYSLNYDDIVDIGWMFRHGHIGLAREKIKNNNSNNTDRQQLYIDPKDLFTIVANTDTELFIHLFEQHKYSAVLYYELILSSKKHIKNVEVVKYLYEHGFARDVTKSFSLAEIDYEVLKYYLENGWLKPTLTMFITYAFYLNNGSKSARSDLKEIIALMIKYVDDKNELILRGTSRQAISLLLLSPIPGLIESLAPYLDQTLKDKTFNEGLTFQQALDTIKEREDFLVSANVDPNSPASQTIYHFKAKMAKLHLLPLNLLVGKRGDEFFTAWNALSGRIRVINNRIYYHTQKNFAKKMKDQNVPSCRDVEDITIHIADIAAINGCLRIIKFLYYDVGAPQPMHMTMEFNVYAHILYPALVKYTSKEDRAFILKLSSINILSQQTLLLACCAEDQYSFKDLCQDVDLLMDQIELDIHKIGFTFDLIQCAITHNDFVGFKHMMSKYIKLPAVYHLFYIQLAASNNLVFIDYIFKNRDKCFPRRIGNKKVNQFFIKMFENAYRCRNIALLEYLIQENCFDIDGMSPKQLVLFNSFIDPPLKSTLNDKHISFHMFVYLVDHNCFDNIKPFYKLLCRQPHYCEYLYRNQQNFANNQLSFQPLFDKIICKYIKTYDKWYYFRDMLGQLAKRYECKLNDSHYHHLVKFAQGLTLNDHVPNSLFLSPNYKIPPKPSRSFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXH3</accession>
    <name>A0A8J4PXH3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH:ubiquinone oxidoreductase intermediate-associated protein 30 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_002867</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CIA30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000084">
        <property type="protein sequence ID" value="KAF2075833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXH3"/>
    <dbReference type="OrthoDB" id="42561at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013857">
        <property type="entry name" value="NADH-UbQ_OxRdtase-assoc_prot30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039131">
        <property type="entry name" value="NDUFAF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13194">
        <property type="entry name" value="COMPLEX I INTERMEDIATE-ASSOCIATED PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13194:SF18">
        <property type="entry name" value="COMPLEX I INTERMEDIATE-ASSOCIATED PROTEIN 30, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08547">
        <property type="entry name" value="CIA30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase intermediate-associated protein 30" evidence="5">
        <location>
            <begin position="23"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2075833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="216" mass="24899" checksum="0D4E230009BF06D5" modified="2022-05-25" version="1">MIKSFTKLFKGSSIRREGEEILFSFREKFDPSKWRLISDKEVGGHSKASFEWDPQEYAVFKGNISKKLPENNPRITSSGYAGVYSPTIDISYLDMERYNTLVLRVNTDGKTYAVGLLKSDERQVMYKSIFTTSKANQWETIEIPLDDFFKIQKGTVVLDLEQMKRENISNIGFIQSERSEGPFELKIDYMKLSTSDLKPTFFTDSRTTSTTGRPLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXL5</accession>
    <name>A0A8J4PXL5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ABC transporter G family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003024</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000090">
        <property type="protein sequence ID" value="KAF2075651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXL5"/>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Pleiotropic ABC efflux transporter N-terminal" evidence="3">
        <location>
            <begin position="51"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="2">
        <location>
            <begin position="130"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="308" mass="34466" checksum="606701ECAB7E4B1D" modified="2022-05-25" version="1" fragment="single">MEYELKDISIPANNDISVSQGREQYDALSNELELESHQYNVSPSDLEANNHWETDEDFKLRKYFENSQRMAMENGGKPKKMGVSIRNLTVKGKGADASVIPDVSSPIYWFASLFKPSSWKKSNGTTFDILHDVNAYAQDGKMLLVLGRPGAGCSTFLRVISNQRGSYVSVNGEVTYGGISAKEWKRYRGEAIYTPEEDTHHPTLTLKETLLFALKCKTPGNRLPDEKKRSFRDKIYNLLVNMFGIVRQADTLVGNEYIRGLSGGEKKRLTITEAMVSSASITCWDCSTRGLDAASALDYAKSLRIMSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXP7</accession>
    <name>A0A8J4PXP7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002969</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000088">
        <property type="protein sequence ID" value="KAF2075726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXP7"/>
    <dbReference type="OrthoDB" id="17470at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045068">
        <property type="entry name" value="BACURD1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING ADAPTER FOR CUL3-MEDIATED RHOA DEGRADATION PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145:SF8">
        <property type="entry name" value="RE57120P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="60"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="168"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="330" mass="37490" checksum="120B72EC77AC8151" modified="2022-05-25" version="1">MTITEPTQIKINEINDMIKELSTLLDEEINLKNEEIKEIEKTRGDILQIKGKIEASLVVNPINLNVGGVKFQTTKTTLTKKSGTLFDLMLSGKGDIKPMIDKPNTYYIDRNGTHFRYVLNYLRDGENVDIPNEIIEDVKKELMYYQITDTLPTTKTNLVKTSNYPHSKILDSYSFKTINDWIDTKTLKMNLLYRASENNFVVQSFHSECDHQGPTITLIETTDGCVFGGYNSQSWNSGGKWYGDDRCFIFTLVNKHGIKPTKYIPNKDGCYVYSSSNCGPTFGVGFDIHISSQPTQLFPSSYQDTTGYGKSTLTPSQNFTIKDYEVYQVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PY08</accession>
    <name>A0A8J4PY08_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003713</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000120">
        <property type="protein sequence ID" value="KAF2074974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PY08"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="91" mass="10498" checksum="1DC7A08314D26CCC" modified="2022-05-25" version="1">MQQQTEEDKLLINTTILSKLIQAQQLHYKNRTECGAVMTAQEEMQMRETFSVFDKSLNLLAKMNAESINQKYSNRSGSNSSLDDSNKFKFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZU8</accession>
    <name>A0A8J4PZU8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Leucine-rich repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_001447</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000034">
        <property type="protein sequence ID" value="KAF2077258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZU8"/>
    <dbReference type="OrthoDB" id="26095at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051716">
        <property type="entry name" value="Plant_RL_S/T_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053:SF71">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035200345" description="Leucine-rich repeat-containing protein" evidence="8">
        <location>
            <begin position="25"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="688"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="728"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="768"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="814"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="868"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="945"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="974"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1009"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1133"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1167"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1133"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1145"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1168"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1191"/>
            <end position="1202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2077258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1202" mass="137117" checksum="2CE089A8D8A02ACA" modified="2022-05-25" version="1">MNKIQYFLILFIVASLLFAGLCDGVKSRNNKRIRHHKNSINNDDPFYRESIKRKEILESFQRSFSQRKPLSIDELTASTGLDSQEKIILESLYQQWDGKDWSNNTNWLQGDPCLNQWHGVTCREFDGVLHVTDLHLPDNGLVGPFPEIFAGLGYMVTFDLQVNHLYGSIPSNVFKDMVNLTDLVIATNDFTGDLQWLRNLPDNIITLWVAMNDFKDTVPDFLSKYKKLDLLFLEENELTGSIPEDICKITSLRLLDFGFNQLTGSLPSNMGELTKLDSFWLYDNTVSGTLPASMKDMQSLKLLDITRNDITGQVPDDLFTQIPNITFIRFTGNEFYGPLQWICPLQNVRELHLDANLFDEFPDCKAIWPLTIENFVASKNHLSGSIPESFGNMVSLNYIKISYNQITGKVPLSFSNLVNLNRLDISENQFNCTLSEIIDPIKYHKHLTILTASGNNITGEFMENMLWDDELEIELLTKMFIINLARNQLSGIIPPFLSWMPTLSDLDLSNNAFVGVIPNELNYLSTLYLENNLIHSPDGALPAFMTNGSDKINLENQAFSCPTIIGKFSNIRISLDSSYYNHSLCSCNIGHRGFNGSCVLCPDNSQCPGGSNAILIPQGYYPLPSAEEPQYLLKCGVSNFGFTPCNPDNEWTYSCKEGYEDRLCSKCSSGYFHRGMECSKCPSGPQSIVIFIIVVIVFILLFMFFVMTDPKRSLPSSTRKTIMYYYQVFNLLLSKLSPWPSFFSAFYSSSSWLNFSFGFLCIGSLSHWPNLFIVMLVLPIAFMVISLVFLGLMQMIQYIRTKGTCGFNKKWMYSGIRVNLLTLNFLYLPLCIYIFQNYTCQKDPFTGTSFMSFFPWVECGNNATYNRIFTLTVLFTIIYVIGIPLLFATLLLYNRKRLDNPTVLLIVGTIYIDYRKSVYWYELINLGKRFLMAVSLALIDPKSSFSVFVVLIVIGSSIIAQIKFKPYVYKISNYAETIGNSVLLFSYVCVLILASLRTTNLYDSRGIEVILSVVVVLYTAFLGLLFLFSLKYFLPKKWQIVIDIKIIKLLDDLKGFSQKYNTQTLSKELSEEEDNYELTKEPIFEMERPSNMMTHSQSIELRRRVSDANILSQSAIHNTNYANDSVRNIKPYTSTPNLSKELSSNNNTNTQPTTMEPVSVTLTKSNTDENINDLNNNNNKIMTNTDDDNYEREQQENKEKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q041</accession>
    <name>A0A8J4Q041_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_002507</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="AJWJ01000069">
        <property type="protein sequence ID" value="KAF2076207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q041"/>
    <dbReference type="OrthoDB" id="10069349at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23804">
        <property type="entry name" value="UBCc_UBE2S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000031">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="9">
        <location>
            <begin position="8"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="168"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2076207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="203" mass="22365" checksum="6F04FC62DC9DB69E" modified="2022-05-25" version="1">MSTELPTDVIKRVLKELKEISTSSLEGITLMPCDQDISNIDACIHGPSGTPYEGGYFKARLTLCSNFPRVPPKANFITKIFHPNVSKKGEICVNTLKKDWSEDLGIKHILLTIKCLLIVPNAESSLNEDASRLLLENYEDYCKHAKLFTSIHASKPIVLSENTNVCESSENVNTSNTTTTTTSNVAAKKVEAKAAPKKSLKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0D7</accession>
    <name>A0A8J4Q0D7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_002271</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000061">
        <property type="protein sequence ID" value="KAF2076414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0D7"/>
    <dbReference type="OrthoDB" id="9974421at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000021">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006693">
        <property type="entry name" value="AB_hydrolase_lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025483">
        <property type="entry name" value="Lipase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04083">
        <property type="entry name" value="Abhydro_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000862">
        <property type="entry name" value="Steryl_ester_lip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035242238" description="Lipase" evidence="9">
        <location>
            <begin position="19"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="Partial AB-hydrolase lipase" evidence="10">
        <location>
            <begin position="44"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="11">
        <location>
            <begin position="111"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="354"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000862-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2076414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="415" mass="46005" checksum="A61C57E3486E656A" modified="2022-05-25" version="1">MAKPILLLLCVLLSCCLAFHQNNNGFYNFDLDSSSGSSDLTRNISQLIQARGYPVEDHTAITPDGFILSIQRIPAGRYQPNPNPYGASGKPVVVLQHGVEDIGTSWVIQENVYQSLGFILADAGFDVWINNVRGTTYSSSNINLNPNDRAFWAWSFDEMAEYDIPTFIDYVLETTQQETVGYVGHSQGTTVAFISFNQNPELASKINLFVALAPVVRVTHCESTLLNILAEFNIDVLFEVLGGKAFLADTPFLQKYLPIICKNQPTICQSSLALIMGWDTTNINNTRLPVYMAHEPGGTSTQNVVHWAQATKSGYQKFDYGAIGNLQKYGQLTPPKYNLTDFSTKTMIFSGGQDFLADPTDVAWLLPQIASSTVYVKNLPTYSHLDFVWGETAYLDVYADATKYLLQYASPSSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0N2</accession>
    <name>A0A8J4Q0N2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001709</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000042">
        <property type="protein sequence ID" value="KAF2077000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0N2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69304">
        <property type="entry name" value="Tricorn protease N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035156596" evidence="1">
        <location>
            <begin position="20"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="312" mass="34543" checksum="677D27FE27E6B8BC" modified="2022-05-25" version="1">MNFKLSLLILSTLLSCIFGQSVYTLTDSTLSSMQWNSTTSQKYNLNQKLFKAHQISSYNNGALQALVSNSNDYLSVVAIDTTSGKLKQLQGMASPSEYQTSEFFDAFYDSSLNLANLLFIGSNTSDPNGPTNVALLSYNFETSQVQTTWLGPKNGTYRGTYDQGEQLYFVVNVVQAGYFQITVLNATTGHVVEKNDIYASVDPTTEFALFARNGGVFVAINLGSKIQMYFLNVNVGTKVSIATVNTPFTITGFDIYLESLNIVDALVYLSVYTNTQSSIYKFTFESMVFDTVYTKTAVLLNSSQLVYIDYNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1K0</accession>
    <name>A0A8J4Q1K0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Trafficking protein particle complex subunit 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001173</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000026">
        <property type="protein sequence ID" value="KAF2077555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6278596at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021773">
        <property type="entry name" value="TPC11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14374">
        <property type="entry name" value="FOIE GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14374:SF0">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11817">
        <property type="entry name" value="Foie-gras_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trafficking protein particle complex subunit 11" evidence="2">
        <location>
            <begin position="395"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="851"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="851"/>
            <end position="868"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1338" mass="150107" checksum="A1CE0F920BBE2930" modified="2022-05-25" version="1">MNELPDDFVNLPTPLVGLIGVPSLHFYIEKYISTKSSIDFERIHPQSPAITSSQQHLGSNLSSSPYPNNSSSPSIDALLPVGIQQSFNNSPLPTSLLAATTLNHSNLDSLGHSPAMVSQQMEDLSLNSNTSNSNNNNPPLSPLPTIVTTGASMSPTSTSHTGITIGGGAMSPNGGWRVNHLNVPVLKVTTIEVGGLSNKKEKKLGQSSESYEPQGILKANWIHKNTSSIPSVVTLFIQWPEDKSPKSHEIILSQIDTVKSNIKNRNIKLMIVIVTSATIDTYDEKVTIMRRKADIDPKYFLFVTKTEIKMFTKKWEKLALELSDLYYKEECHTTKSQITKTTHPYLNVRYQFKIAYYSEIRSDFNTSLKYYTFAYNSLMNSLKDLKPTNDPKGIRFAEFRTVASFLNFKISKLSMWLNNITEALQHFDKHIKSFKIYLGPAEKEFTHATWISREYQIFAELLELCPGNTTKTINYNNNPGFYYQVSARYQSIRRDLFKSLSDNYKSSSHASKFKGDLKPRYDLALCTFIGQPPPDVAHPLEGSSGNIMTNDDEVDHDFYRSIAIELHINYTSLIIELYSKAYEQSSLGGNFRILSYIESLIAYEYYISKRYDLSLKYYSKNAFAYRREKWWTLLTHSLSMILKSVHQLNMPTNYVGYAMDYLSPDLSNSYIERSNIQQSLLYILTDSSKLSPPLSLSSPLEVNMEHTHPLLNCRVQFPQSFTFTHSKTEFYVVFESHFPNPIRFSSLKVKFNEKSYNKQISDPIPIESVISPSIQRDAQRDDLVFLPNESRLFTFTLTTKEKMELECQTVTLELGNGNSINFIWNIHEWAIKHDEISEDINSDASLLLSPSLSNNNSSNNSKSNNKNNSSKKKETSKPNPYKKFLERSTIRILDHESLIQIKCNHNSPAIVNEYYEVEIELINNDKDIKKGTVLFEINQQQNHTTIIEKGIYTEANKKSTPLSSLELPAIAEKASFKKKFFIYCSGPDENKLTINVSYETKNGEISHSSKIFNIPVLVGLSPQFHFYNDNFQLCEMYAGGNGETDANGPNVGGTPGSGASVVVGANVGGGVAVGNNTTGSSNSSNTTNTGSIVQKEPLLLMSEIRSTLPYSIVIEKVTLQIASIAESVSLPLSPPPIGQSSDDPNLSMPVAQLLSNSNANISSVDLSKDTNYSVWFNIIPLITGESISLGTLLIEWRRKSGSIVSKLPIPLPHTRITTNPFHTDVVVPSHGVVGEPLSHIIKISNNTNFLQEFDLVVINPPSFGSSDIPFYFSGDRSSSFSVHPESTYEIKHILLPLVAGKHPLPHFKITSKRFNKELAKTQNANFLFIKPNLEWVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q259</accession>
    <name>A0A8J4Q259_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ADF-H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000186</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000003">
        <property type="protein sequence ID" value="KAF2078563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q259"/>
    <dbReference type="OrthoDB" id="20462at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829:SF54">
        <property type="entry name" value="ADF-H DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829">
        <property type="entry name" value="CORTACTIN AND DREBRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="2">
        <location>
            <begin position="411"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="362"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="591" mass="66816" checksum="068521D5376410D1" modified="2022-05-25" version="1">MQSPTPNNSLVINQFQRVVSKYKWTIFNNDLSIVKSGNSVTELSSFFKLKNCLQILIRNDTVLKKNNNNNSKNYSDAMHDIYAYVYSYWIGPSTSSQDKISFQESFLQNQKSLPKIISIVNVFQFCEKYIDEQLFINSMKINMYQPLFINVMDNINNIPINDAPETNISRHIQKMKIHQWISLQSNKRNQVEFQIGNTQENLEKLLGIGCTNITIFKKSFKELLYVVWEGKKSKPIQKIKFDQFHHFIYMMLREFHGSLKVLKFSSKDEFNLLMEIVNNEHAAKNTPGSAASKFVTNVSSGSSCPPSKPSTPLYRKPRNEGSVPLTGQRRNVVPHCSPVSVKSRYTPISSVCNSIASSPATHRSIPSTPAVGDEDDTLSQCSASSLSPASSNIQLALIPDEKKNKTVVLYGVTYQDNDELMNTIDQFNKKTINWFLLSYDTKRNLVVYGKGQKGVNEMKSHLKEERSLFGLVSINYIESNPEQLSCSSESNKSATLKKSNGGNGHSKTLFVQWLGKKSSALEKARRNSHLSGVSNLFKQHTLVHGELEADEVEDLNNSSILQKLTSFRNDISNTHVLYDSCEALLPNLNVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2P2</accession>
    <name>A0A8J4Q2P2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 2">G protein gamma domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001083</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000024">
        <property type="protein sequence ID" value="KAF2077620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2P2"/>
    <dbReference type="OrthoDB" id="19232at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015898">
        <property type="entry name" value="G-protein_gamma-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036284">
        <property type="entry name" value="GGL_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00631">
        <property type="entry name" value="G-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01224">
        <property type="entry name" value="G_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00224">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48670">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G protein gamma" evidence="2">
        <location>
            <begin position="2"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="G protein gamma" evidence="1">
        <location>
            <begin position="6"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="69" mass="7731" checksum="93756601C60254F0" modified="2022-05-25" version="1">MSESQLKKVLKENEALKAQLERSTTILKVSEACESLQEYCTKTADPFIPGWTGENEWTKPLKGNVCSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3H7</accession>
    <name>A0A8J4Q3H7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_000606</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000012">
        <property type="protein sequence ID" value="KAF2078055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3H7"/>
    <dbReference type="OrthoDB" id="341482at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037700">
        <property type="entry name" value="NUP88/NUP82"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13257:SF0">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13257">
        <property type="entry name" value="NUCLEOPORIN NUP84-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="635"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="820"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="546"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2078055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="873" mass="99988" checksum="33DC32D0405668CA" modified="2022-05-25" version="1">MKIREIITLLSSNPLFQNDQSYFTKLVNSSNNSNNKRSDGDDSNNPHLKSPKNFISINNQSSEIYYFSVSTSKLFIIKVESTLDNTIGAIKFQELKINVNFEVYKIELNENSRYLAIVGQVNCMIVDLSPSITNQYFSGKVIFDEPESKTLGYDTLLTRSFILDKFFQLSQMSCIIKQVEWHPQSNIHLLILYSNNLLKMYNIQNENDHCEQNINLESLHTWSSLNLIKTNNVKQQKHDKERFVSFCFGANINFWTRFTVFLITDNGDVYNLCPIIPNGCLVESSFYKQFQKHIIRQKEQQQQHSGGNEYSKSYFDYQFKWLMAVTINNVIVEIENSDWIKFSLPLQSSAFSPPLLQGPVFQTSNNLINSSSAAGLASTTSKTLALTSSNNNSSSTSDKNQPIRIQCISPMTSAAEQYKQQNNNSSSSNLPLTPFILIILLKSGECIVCVSCQDTIPMWSTIENNSGIYFEQDTSFPRFLDLIHYETLDLELTSTSTTTKSKPNYLLYSYPSMKFDQFTNSLLFFHNSGVQIAKFPWISQLFQQINPNLIQKKQKEQQQEEEEEQQDLEFENTNLISIVQQPPNNSIIPIVGIDVFSNIKIGTYLIIVPNTFNIIIFDMDGLCLTTDSFDDSVYNNNSSNNNNDNNNDSTNDNNNESTLLPLQQIISPLKNYISNPPPTIPRITLPHGNLKAIDFLKTLVEVKSKVFNAVSYSSQLEFDLQARIKGLELLRKEQLDTLTKVQLYINNIKHNQTLLNNKLTYVWDKQEVSSEQMNSLYQIENEGTPLSINEIDLKKSLKQMNSQISIYSEKINQISKDLELEDDNNNNNNQGSTEDIPMENQKEIKNLLDYQDTRIDDILNKVEKLIKSVQITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3P0</accession>
    <name>A0A8J4Q3P0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Xrn1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_000544</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000010">
        <property type="protein sequence ID" value="KAF2078160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3P0"/>
    <dbReference type="OrthoDB" id="21527at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027073">
        <property type="entry name" value="5_3_exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004859">
        <property type="entry name" value="Xrn1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341">
        <property type="entry name" value="5'-&gt;3' EXORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341:SF70">
        <property type="entry name" value="XRN1 N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03159">
        <property type="entry name" value="XRN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xrn1 N-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="372"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="679"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="753"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="117"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="679"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="702"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="753"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="774"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="811"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="835"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="862"/>
            <end position="875"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2078160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="875" mass="100986" checksum="77CBFE3AD9B31BE1" modified="2022-05-25" version="1">MGISDITRFINKTYPQIIKNTVQGGGGGGTKYEHVYFDLNNLLYQFTVGKKKGNAQSVLKGLRFSLRKLILQNPPRKSLFLCLDGPGPRSKMIEQKKRRLAKSENAKVIFLAKKLYLEKLRKESNKEEIEKVEKEIQQDEENQDDNDDEDDEEIDFDFEKILRTEGEGKILNQGYPDIDIPNSILQPEDFSSLNFTPGTHFMVDLKNDLINFLKTSDIRTLSKLNDIYISGADRLGEGEWKIIQHIKKMNHPVDSPIGIISNDSDFILYGLINNFNITFVSPTFTIHIPTLKQSIQDSVPLSKDPEQVIVDFVFLSLLTIGSDYLPGVRDWGFDAIWEQYRKTPLAIFDLQSNTINHKTLLQLIETNSNSSPYSNRYGGGSWNNKNKNNDTESESTFYSNKLSQLITFKNNSYPKVNGGVIKEIAKVVPGPRGGFSLTINDVEIDSHKDKISLYKKFITKQHPYWEQHLPVIQQSPKYVYKVDLIFDEVKYYEKTTTTDRTPLATNANSYVYGLLWQMKYFQGVCTDFNYFYAFNSLKLQEILSCTAIFNDIKPPGPVKSSPLIPLYFSFALLDYSSKYLVPSIYHPIYKLHPYYNLKRNIALKSFTKQDHLDHLVNLLDIHTKKGPLDEKFKSQLTFNPTVLFKVKGDKVEEYKELNCHQAIDTHQYELVNTHKDFSFQTNPNASYRPYNNQHQPKSFHQNNNNYNNNNNNNNQSTRSLRYSLNNLSVGSQKQQQQHQPIVNINQPMNHFKQQQHQPHVNFNHPNHFRPYPQPQQQQPQQQQPQQQQPQQPIQPQAPKRYIRKTKEPKDTTTTTTTTSTAPAAAPLKKEKKTKTKESNIEEKRPSTIQQKSSDKPLKRIKTSNNTTTITTTSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4Y2</accession>
    <name>A0A8J4Q4Y2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Argonaut-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_004672</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the argonaute family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000169">
        <property type="protein sequence ID" value="KAF2074021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4Y2"/>
    <dbReference type="OrthoDB" id="445936at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02845">
        <property type="entry name" value="PAZ_piwi_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04658">
        <property type="entry name" value="Piwi_piwi-like_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000014">
        <property type="entry name" value="Piwi-like RNA-mediated gene silencing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23278">
        <property type="entry name" value="Piwi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00949">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAZ" evidence="3">
        <location>
            <begin position="405"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="4">
        <location>
            <begin position="648"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="46"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="125"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2074021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="964" mass="109565" checksum="DDA8658FB4822576" modified="2022-05-25" version="1">MSHHGKGKGGKGKQEPEPPQPLRIAKRPPQPASQEEEEGFPALGAKQPSPSKQQQPKQQQQQQQQQPKQQQQQQPKQQQPKQQPPKQQQQQQQPPKQQQQQQQQPPKQQQPPPGFAPREYENPYQQQQSRPGTTPQPSSSSAPSWGTSGSAPSWGSSNAPSAAELLKQTQTQQAPTPKPATTTTTTTTTTTTTSSGAAQQQPSTSTTGQSVIPSNVPRGRLRPEVRDIDVVGLAPQTGQVGLHRSSRRVNIYTNFFPMIFKNRAIYQYQVDFTPEQETRDRRTYLVSQLNFPVFRYDGSAQLYLPIRVEDKGVTIDGIRVQIKMVKPISSQEKEAVQFFNILTKKFFFLMGYSMIGRNYYTNVNAYFMKEYNLQIWKGFTSSVSFIEEGAALLCDMSSKVVRSIDVLQKFNELNFARSPCENVVENAIVLTRYNNKTYRITGVAWNVKLTDKMDANLPSYIEYYRQHYPKYPIKDLKQPLLETQIRFQGKPKKAYLVPELCHITGLGEEISSNFHVMAEVAKFSNVEPPARIKELNDFIGRLPANDKVAKELDDWGVSYNPSLKVEGVCLPPPQLHVFRNTLRDTDWVFLVSKDDYAKCKDTVSILTKETGLPDPIVVHPTIVRRGRDIDPRGYINELQYYAQKDIKLFIIIIPQNAKDIYNVIKQRCLMEFKVLTQCIYLRTFLKGRPVAQKLNQQMVAKMGEAPWILKNPAHPNIPKKTMVIGIDVGHNSDTNGRSVVGFVASLDNDYCAFYSKAVVQSKSGKEIIDDSLYGCTLEALKRYHKKNNTLPEVVIVYRDGVGDGMLKLVKDFEVEAMNRAFIDTPSIYGTKPKLVYIIVKKNTNVRFFQGDSLQNYNQFRNPEPGTLIETKVTHPGWYDFFLVSQVAFKGSVNPTHYNVIRDDAQIPRDALQQFTFAMCHLYFNFDKSVRVPAPCQYAHRLAFLIGRNVHHNAADELAHNLYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q5R2</accession>
    <name>A0A8J4Q5R2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome b-c1 complex subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_004259</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UQCRB/QCR7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000147">
        <property type="protein sequence ID" value="KAF2074441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q5R2"/>
    <dbReference type="OrthoDB" id="425749at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1090.10">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003197">
        <property type="entry name" value="QCR7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036544">
        <property type="entry name" value="QCR7_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12022:SF0">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12022">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX 14 KD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02271">
        <property type="entry name" value="UCR_14kD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81524">
        <property type="entry name" value="14 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2074441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="12850" checksum="43F4AEB67BFE2FBA" modified="2022-05-25" version="1">MNIFKLLPESFVTKLQKSSWMEHRKLGLFYADLYNSTPVIEEVYSRLPDNVLAARDRRLRIAIDLSVKKQTLPEPEWSDLVADAQYTKMIESYAESVQKEHDLRKSFRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q7V6</accession>
    <name>A0A8J4Q7V6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002467</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000068">
        <property type="protein sequence ID" value="KAF2076226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q7V6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="182" mass="20797" checksum="AC33C5E13143A9B1" modified="2022-05-25" version="1">MDDEPNLKEFRKTLEELLNANETNKTCLDFIKNKNVLYDKLFRFTLFEFSQCVQDLTVRINSGDISILQMRQTRYTEKDIRRLVLKIAKAQFNISPADSNSWLENFLDDITFMHFAREFLACIIEYHNEDVLTTSIGGDSFSTANGLSTNSFSTSSFSFSNSITSNNTSSSSTHNTSFSKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q8F6</accession>
    <name>A0A8J4Q8F6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">4-nitrophenylphosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_002004</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="4">Divalent metal ions. Mg(2+) is the most effective.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000052">
        <property type="protein sequence ID" value="KAF2076691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q8F6"/>
    <dbReference type="OrthoDB" id="413953at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000280">
        <property type="entry name" value="HAD-superfamily hydrolase, subfamily IIA containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006349">
        <property type="entry name" value="PGP_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01452">
        <property type="entry name" value="PGP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF46">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000915">
        <property type="entry name" value="PGP-type_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="29"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="31"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2076691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="300" mass="33629" checksum="1FA0CBCED6545FEC" modified="2022-05-25" version="1">MEKTTHVTSELNINNIKSFLEDIESFIFDCDGVLWRADEAVENAAQTIAFLREQGKKVIFLTNNSTKTRNDFLHKIKSFGIDCNIDEVFSSSYGAQVYLSKINFQQKLFMIGEKGLEEELRPLGLEIIKNFEKTNDGIDNVQHIEPDHSIGAVIIGMDTSYTYQKGVYAHKLLAENPNILFIATNTDPSYPVKNGKSLPGAGSIVTMIATSTGRKPNAIMGKPEQLLLDLILESHSLNRAKTCVVGDRMDTDILFGVRGNTKTLLVYTGVTKKEELPSFEYQPQYDTRTIGDLFTLFQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPA0</accession>
    <name>A0A8J4UPA0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">alpha-mannosidase</fullName>
            <ecNumber evidence="4">3.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_009755</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.</text>
            <dbReference type="EC" id="3.2.1.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 38 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.24" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000796">
        <property type="protein sequence ID" value="KAF2068926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2016903at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000001">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.110.10:FF:000012">
        <property type="entry name" value="Alpha-mannosidase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.50">
        <property type="entry name" value="Glycoside hydrolase family 38, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007246">
        <property type="entry name" value="Gaa1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041147">
        <property type="entry name" value="GH38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011682">
        <property type="entry name" value="Glyco_hydro_38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015341">
        <property type="entry name" value="Glyco_hydro_38_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037094">
        <property type="entry name" value="Glyco_hydro_38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028995">
        <property type="entry name" value="Glyco_hydro_57/38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050843">
        <property type="entry name" value="Glycosyl_Hydrlase_38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607">
        <property type="entry name" value="ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607:SF19">
        <property type="entry name" value="ALPHA-MANNOSIDASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09261">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04114">
        <property type="entry name" value="Gaa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17677">
        <property type="entry name" value="Glyco_hydro38C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07748">
        <property type="entry name" value="Glyco_hydro_38C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00872">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88688">
        <property type="entry name" value="Families 57/38 glycoside transferase middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="300"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="404"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="445"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="472"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="502"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="542"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1015"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 38 central" evidence="13">
        <location>
            <begin position="838"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2068926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1457" mass="166996" checksum="F735DB66FB1172CC" modified="2022-05-25" version="1" fragment="single">KVGVNVIGINQAINSLGTESFVFTTSFDQWHSAGSVGFLLGFSKFIKQTRWQARDIIYVFSTEGGEDNGGTSMDISGISVWLHDYHSSNNINIQNLNYMTTIKQKGWTENNQRIMRAGQIYGAIGFDRIGRNSNNNINNNNNNNNNTDSNQQQKVVVYPEGLEGSLSNLDIINVITTTSFLNDIPAGINTHLQNEPVEGSLYGLLVFMWNSALSMPRSNHAIFTRYGINSVGISTDSSHNVWDFDFLNTPFHHKFKNRLDNNNSLSSFNNQTFYNLGKIMETVIHHLHNADEQLHQSYRWYMMAGAVFFIDLGQALLPVIVFNVGIFILLISLGLSTNDIAIKILLSLPWFIATLIYCLLQLCLPKLLSSNFISILPNRDLNPSNLLSFDWIQGIFKNNIENQIIGFSILYLVLCFIIYLVILLPISRYFTNSLLKQFPQYKNDWFGLQTILLLYYSLLGLMGMMLNNQFTCLFIVLSLPTLHFTCYLLVRNTKSIILKSIWMLVCLISHPIFLVFEFWSIWSIGWKKLFIGILLAYDNWGYLFYPFFIFGLQPIFFVRQLVNNKQLYFIGGGWAQNDEAAAHYEAIINQMTLGHQFLLSEFGVTPSVGWQIDPFGPSTLTATAFSLMSFKYHVIDRLDDRLKHIYTNTPDIKNSGSMISNQDFEFIWYPSNNYGRELSIFTHILDNMYTSPSLCVYPNASDPNFSICTGFDFEGDPDINPPINESNINERASILVEIIKQRSSYYRHNNLLLPFGSDFRFQDATVEFINMDKLIKYINTNTSFGVNIQYSTLDDYFDTVFQEVVNVNETFPEIQGQDYYTYTMCLTTDYQLYNTCVNYWSGYFTSYPLLKQTARDSDSLLRNAEILYSLASAYGNGFDFDFTPSYESLSYHRNVSGILTHHDAITGTAKEYVRDDYFQMLYQAQNQTLQNAIPSLVEFLLANKSIPFNYTTDSSILLDIKNDDVYAVSFTNSLAWDRQEFVCLEISNQYIAVYNDKLESIESQIVNRIDKGGKFYLYFMVNTPALGINTFFILGLSSTTNSNQFIFENSNIISKPILPFYSNNINIKSSSSISIGNSLFNLNFKFNTKNNNILRLESFDDLARNIKSIPLVQELIEYESKNDDAYKFRPKDYPTTLLAETPQFYYTQGPIIQLITIVYSNNCSQSFSVYNSTFNNGSFLNSNQYFEIENIVSVGWNKEISMRFNTSINNGQTFYTNNGVEVLQRNFIEKFNDSSPSSLISGNFYPVINTGYIVDQHNQLTVLSKQTNGASSQDNGVFEYLLIRRSNFTQWSVGEPMNDISNPSLKFRILFGTPNNVESIRTPHSIIFENPLMPVFTSSVQQVGLLKWINTFNTRFEPLTHSFPYNLHLVTLTKQWIDSSTNIIRLMNIYEINQNQQYSQPLNVNINSLFNLNFNISAVYETNLSANQKISNLEYDILTTTLNPIQLKTFVFELNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQ47</accession>
    <name>A0A8J4UQ47_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_008513</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. ARD1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000531">
        <property type="protein sequence ID" value="KAF2070171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQ47"/>
    <dbReference type="OrthoDB" id="25586at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990189">
        <property type="term" value="F:protein N-terminal-serine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990190">
        <property type="term" value="F:protein-N-terminal-glutamate acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000037">
        <property type="entry name" value="N-alpha-acetyltransferase daf-31-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045047">
        <property type="entry name" value="Ard1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091:SF4">
        <property type="entry name" value="N-TERMINAL AMINO-ACID N(ALPHA)-ACETYLTRANSFERASE NATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="5">
        <location>
            <begin position="2"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="151"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="151"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="172"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2070171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="206" mass="23406" checksum="C9A323D95FCC7177" modified="2022-05-25" version="1">MVSIRPCQIDDLMSMQNANLTCLPENYQMKYYLYHFLTWPQLSFVAEDDKENVVGYVLAKIDENDVKRGHITSLAVLRSHRKLGIATKLMKQAETALLEVFDADCVSLHVRKSNRAAFSLYHEILKFKIEEIEKEYYGDKEDAYSMILHLKPEKSKKDNKKEDPKKEQTPASSTSKTQTSNTTSTTKGNGPQQPTKAGAKGKKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4URC4</accession>
    <name>A0A8J4URC4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006708</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000321">
        <property type="protein sequence ID" value="KAF2071971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4URC4"/>
    <dbReference type="OrthoDB" id="19802at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05379">
        <property type="entry name" value="CAP_bacterial"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31157">
        <property type="entry name" value="SCP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31157:SF1">
        <property type="entry name" value="SCP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035309086" description="SCP domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="2">
        <location>
            <begin position="28"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="2">
        <location>
            <begin position="154"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="283" mass="30842" checksum="F5F250ABCD94B759" modified="2022-05-25" version="1">MKIISRLFVLLLVLGVCFAQTNPSYHIQLVNNERNKLGLAPYFLSPCLVDAAQSHANYQASINNLTNDSPYGGLFARVNVYGAYGNSIAESVAYGYETDDQMITDVMKNPGLKGNLLSSKYNHFGVATALGSDGRYYWTQLFLVVGQTNPAIHLQLINKERAKVAGLSPLVLSTCLNNAAKTQSNYQAALNQLTTNSSIRGATLFDTIKQYGESGVEVAINVAYYYTTDADVIASLMNSSSKANILSNKFNHLGVATSLSSDKTVYWTQFFVKSGVCTPPKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4URP9</accession>
    <name>A0A8J4URP9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000270">
        <property type="protein sequence ID" value="KAF2072541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4URP9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035310583" evidence="1">
        <location>
            <begin position="25"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="659" mass="73024" checksum="985E704B8957F292" modified="2022-05-25" version="1" fragment="single">MKGYCYFFILFALVFLSSIQVSESVTVDSIRYKPYPIYTTDTTCSYVYEVAFQDPKGGIQYVSTPFVSTFSTLAISGTLLKAQIVFKPTLATSGDLVVSLKNSEGTTFSVKVDAYQCLAVPEFEYDKTSAPVITGYGLFYYDLKITTPMTQYLNMDQLKHTSSPYSSYVKIMGLQQYRIYYALLGAPPTTSHISFSIIVNLKTTPNTDIFITIPSPFTPSAWVGTSQYSTSPINPDYSFTTLSYENNLNGNLIASNVTGGYYSISRPTEGNTKKGIYLIHMNRKGLAASTVSIKEDFNTITANAPLNLGKTDKTLPAQTSIAPQSYALSFIDALNRPATTFFNAIYNSKAKVFDSTQSYLIKRGQVEAKKYLTYPYSYSLGNIVDGYKVSFSYPTWNFAGTSLLVYLQLNGQTQQWITKSNPTIADITITSSCNFIAQVGRKRIYRLNYSSASPFDSVVSPPQFGAVVIATPANIVSGNEYNGAIEFNIENPTFDIVRVVNDAGNVIQQVLPNPIPSIFTTANIKGISFSTNNIDTTLLTKSVPIRATLKLDVPDLVFRFLLYSTDFFPTNTYSTEGYDVFYNPTTQEYIVDFEIHPRRPVYYVFSNTQLASITSLTIDTMFPQDTLKVTYENVDEMPPMVTSVSIVEGNTLAVDSSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4URX4</accession>
    <name>A0A8J4URX4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005819</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000242">
        <property type="protein sequence ID" value="KAF2072871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4URX4"/>
    <dbReference type="OrthoDB" id="364348at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF345">
        <property type="entry name" value="T-SNARE COILED-COIL HOMOLOGY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="2">
        <location>
            <begin position="21"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="119" mass="13671" checksum="7D3AD983473BAFDE" modified="2022-05-25" version="1">MSYVPLREPLLSQDSNYEYEDTLIYERNMQTRELVDDLYNLKGAMDDLKILTDEQGVMLREADDHVMHADLDVEEGVEQLEQAYEYKSRSRKKIIIILVVVGLIIGVAVLIVLNTLGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4USG7</accession>
    <name>A0A8J4USG7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000194">
        <property type="protein sequence ID" value="KAF2073606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4USG7"/>
    <dbReference type="OrthoDB" id="21679at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="342"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="409"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="480"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="575"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="132"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="411"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="480"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="577"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="686" mass="75496" checksum="00D66144A0E10A80" modified="2022-05-25" version="1">MEALMELSNQEVLSLSTDKKQNPLVLACQDQQNDLKEEDTEEDEKHMSSSKKSPSSSGKKKINVDEACEMVIDDVVVIEDSNQSSLVSGSSTSTPRASSISPGKSTSSKVAKSSTAAGKHKKSNSKSGDIGGSDSTPSTPTTPSGTLGKKDKDKDLENDRKRQSFMLHWRFKPSGNSPTSSPQLGPSNSGTVPNIPNLQPLNPTTAPIEAPTTPPPMSPGGGENYNRNRRNSAPTIQNYVSHWKYDPKAHQKQQILVQHQQQLYVQHLQQQQQQQQLQQQQQINQINQQQQQQQPIFRPISPEELNEQQQQDLMNGKPSLFHPYTRGVSPLNLKSVQQFCCSPNSSPRYQSPDQSPIYSSPKPERRFSLPNVPSYTTAYQMNQQQLLQQQQQQQQQPIHIYSSNPPCTGSCCPQQQQQQSLQPPPPQQQQQCSSPSMGPIVNRNNLGSIPEVPNGYHVEYSASPSNNNLFIGLVSHSPPFSPSLSSSSSHSPPMVPSVVPNSNNNNTNNTMVPVLYHSSPKSSPLLVSNTNNNSTPRIISKPQISLSSEMSSSPRLISQQQPIVPQNGCSSSFISLDNNTNSNTNANNDSKDDLDQKLPSMRDLLSHLYIKDTNPYVRPNNNQQPQNIMVSNQSMNISNNSNSNNIHIINNNSNNNNNNNNVVVSQSPPGENATTANPMCIINLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UU04</accession>
    <name>A0A8J4UU04_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010246</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000993">
        <property type="protein sequence ID" value="KAF2068428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UU04"/>
    <dbReference type="OrthoDB" id="417426at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="993"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="993"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1021"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1034"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1034" mass="118841" checksum="F4981D7612A6A94F" modified="2022-05-25" version="1" fragment="single">RAYKRKVSNYERNFIKECYIVNDDNIEDQIVLRTHIAWRSWKLPKVGQIPDGVKKITFEFINEPIGKGIIPESVISLVFINLNICLSTISFPTNLRHIDLGPRFKQFVFCNQDLPMTLRYMEFSIDKHDSSNHWLSYNGTLPFGVKYLKIKCKGLDGDSQLFKLPFWVEAVQLQTKDLFEALDSFEIIKQNVYYDQAGSRELIPFHPALKSITISYYFTENFSLNTFPSTLEYLDIRKNFDYSHHQIPSISFKYLPASLTSLKCREILGEFPEHLKLNYLKYSIKDCGIKKIIFSVSDRSITKYNTPTSFDKNTGLFNPQPTTIIESLCWDGSTLYPTKNAQVKLLTNSNQYYSGKVQLESTQALVYNILPSNYPESLEKVTLRRPINNNQFYSIPPTVKVLQLTENNINNLLLFPNNYAKESITAPISNNLSQINVDNHDLFFKIIRNQYLKKLVFDSISPRIIDFINYTEPAKRKKAKGSLKSSSSTLEIKSKSVDMLNDLKDKIPTTLKRVSISDKLFMGSKIEATPAFLYQNNLLDHVYQVYKKEIPISSTTTVLIWEENVPITLGIIPFGIKKIIFGNKFNQTIIKDSLPCSINELSFGSGFTQCLSNICLPESVRYVSFNKYYQNLNPSIFPAFVTHLSFLSYTNTKDLVKFFSKIPISITHLYFKFKTSGGKQKHITKIKYPTPQYLLYLLTTQDKEENDDGDYDNFMSIDNSNDKVVVPSFEIHLNYESDSSIPVGSLDHLNVKSIKFNSFNQALPKGCLPNSITELEFQGFNQFVFVPPHSLKSLSLSKEFNQELNSSMLPPSLERLSLDTDKEIQIGVIPHGLKYLDLGSNYKHEIKQGVIPNTVEELIITIYDNNLNTTSIPNSVLKLQLKCNVDLKQGIIPSSVTKLCLFECLTNISDIIPKSVEDLEINNVEDESFECKWIPCSVTQLSIKTNILVNLHELPVSIKTLTFESKNYFGQIPSTIENLQVLNYSRHIRIYNEDYEEDDDKDEEDDDKDKNDRDDEEKEKDDDKDIKKDKEANE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UUP6</accession>
    <name>A0A8J4UUP6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002979</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000088">
        <property type="protein sequence ID" value="KAF2075736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UUP6"/>
    <dbReference type="OrthoDB" id="1893551at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF122">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN SKYWALKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="211"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="8"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="74"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="142"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="377" mass="43566" checksum="E8A3C29C6B54539B" modified="2022-05-25" version="1">MQIPSNYIDGIINDNARLQRENTELQREILKYKNAGCSNKDHISLLSEIENKDKKLEKRDRIINNRMGLIVTLYSGKIDQIDELKQEINKLLMERMDTLVEENNRLKSDLDKKNQIMNENLQSLYCSLLEEHSKLKSKLETITSFDEELKSLKSKNKKMKKKKESLETKYALLISNHDEKEKEIQEIKEKLSVYEKAESKTPFKNIVLESKIIDYISFKTINDWIDNSKNYSFKIIYRASNNRFSTQSFHSACDGKGSTITLIEATDGCVFGGYNSQFWNSNGKWYGDNKCFIFTLVNKHGIKPTKYLPDPTKKCYVYCGSSTGPSFGFEDINILGQKGNQSFPCTYIDTTGKGISTLTPSKEFIIKDYEVYQCLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVG1</accession>
    <name>A0A8J4UVG1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_010052</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000903">
        <property type="protein sequence ID" value="KAF2068625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVG1"/>
    <dbReference type="OrthoDB" id="15567at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031941">
        <property type="term" value="C:filamentous actin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051371">
        <property type="term" value="F:muscle alpha-actinin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09338">
        <property type="entry name" value="LIM3_Paxillin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09334">
        <property type="entry name" value="LIM4_PINCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000008">
        <property type="entry name" value="Paxillin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000009">
        <property type="entry name" value="Paxillin isoform 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050604">
        <property type="entry name" value="PDZ-LIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214:SF62">
        <property type="entry name" value="LEUPAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0440">LIM domain</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="11">
        <location>
            <begin position="302"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="11">
        <location>
            <begin position="361"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="11">
        <location>
            <begin position="479"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="120"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="167"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="244"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="171"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="244"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2068625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="537" mass="58995" checksum="8F8A1BF39386244A" modified="2022-05-25" version="1">MDDLDILLADLGRPKSGVKTNPTPSSNGKAFETNDIHNEIQSIIEELDAQPQQVVTTTTTVTPIKANFSVTVETPAPAPVVNHSHQHSHNNNTNTATNIEGADDLDELMESLNSSITTALKAVPSHPEESKQPSYSKPADNNSPTQTRVTSTATMVKKPVVVSKAVLETTAPKQSNPVTTPTPVSHNKPTNSPSTDDLDELLKNLSPSQPATIHVPAPVSVNSKPSPTKPYQPEVVNHTTTVHFNHNQNNNHNHNHNVSNNNNNNNNNNKGKVLQGDDLDNLLNNLTSQVKDIDSSGPSSRGTCFGCKRPIFGEVIQASSRFYHPEHFVCNNCQNPLGTRNFYEQDNLAHCEKCYQELFCARCAHCDDPISDRCITALGKKWHVHHFVCTQCLKPFEGGNFFERDGRPYCEADFYNTFASRCGGCNGAIRGECINALGTQWHPEHFVCQYCQKSFTNGSFFEFGGKPYCDIHYHQQAGSVCSGCGKTVTGRCVDALDKKWHPEHFVCAFCMNPLAGGSYTANNGKPYCKNCHTKLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UW08</accession>
    <name>A0A8J4UW08_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2'-phosphotransferase</fullName>
            <ecNumber evidence="3">2.7.1.160</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_001623</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2'-phospho-[ligated tRNA] + NAD(+) = mature tRNA + ADP-alpha-D-ribose 1'',2''-cyclic phosphate + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:23324"/>
            <dbReference type="Rhea" id="RHEA-COMP:11106"/>
            <dbReference type="Rhea" id="RHEA-COMP:11107"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:76596"/>
            <dbReference type="ChEBI" id="CHEBI:82883"/>
            <dbReference type="ChEBI" id="CHEBI:85027"/>
            <dbReference type="EC" id="2.7.1.160"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KptA/TPT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.160" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000040">
        <property type="protein sequence ID" value="KAF2077056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UW08"/>
    <dbReference type="OrthoDB" id="419694at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000215">
        <property type="term" value="F:tRNA 2'-phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.170.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.970">
        <property type="entry name" value="RNA 2'-phosphotransferase, Tpt1/KptA family, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002745">
        <property type="entry name" value="Ptrans_KptA/Tpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042081">
        <property type="entry name" value="RNA_2'-PTrans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042080">
        <property type="entry name" value="RNA_2'-PTrans_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12684">
        <property type="entry name" value="PUTATIVE PHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12684:SF2">
        <property type="entry name" value="TRNA 2'-PHOSPHOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01885">
        <property type="entry name" value="PTS_2-RNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2077056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="218" mass="24970" checksum="4F689E6F6851264C" modified="2022-05-25" version="1">MASVPNKGINKVSVSKSMSYILRHGAEKEKINIDKEGFVLLDDMLRHALFKNVTVDTIIDIVKTNDKQRFFLEKRPQDNRYYIRANQGHTLKQVDELELRKLEDPGEVPMVIHGTFKKHFDSILEKGLCKMERNMIHFAIGISNDVVSGIRTNSNMIIYIDLARAMKDGVEFFISTNNVVLTPGVDNSGVLPSKYFKKITDRNNVAMWMRDVVMPQPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWF6</accession>
    <name>A0A8J4UWF6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PLD phosphodiesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_009039</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phospholipase D family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000623">
        <property type="protein sequence ID" value="KAF2069645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWF6"/>
    <dbReference type="OrthoDB" id="1923775at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09106">
        <property type="entry name" value="PLDc_vPLD3_4_5_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09107">
        <property type="entry name" value="PLDc_vPLD3_4_5_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050874">
        <property type="entry name" value="Diverse_PLD-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032803">
        <property type="entry name" value="PLDc_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10185:SF17">
        <property type="entry name" value="GM01519P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10185">
        <property type="entry name" value="PHOSPHOLIPASE D - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13918">
        <property type="entry name" value="PLDc_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035285909" description="PLD phosphodiesterase domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="3">
        <location>
            <begin position="154"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="3">
        <location>
            <begin position="364"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2069645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="429" mass="48127" checksum="E55C91503255DC88" modified="2022-05-25" version="1">MKSTILTIALMGILLISASNGINVSGSTHKIKLQDGSCDNGSISIAESIPLFLNFSTPLSTHQAWLNLINNAVESIDMGIFYMTLTDGSSLPSEYGGQLGMDIFKALIEAHSRGVKIRIVQNQPSASMPANDTNALANMGAAEVRSINWAKLVGGGILHTKVIVVDQMNVYLGSANCDWRSLAQVKELGVVIENCKDIVEDTQRAFEQYWDAAAMTTLPKEWPMKDDAKYNSKDMAKLILNGDQVYNMFLAVSPPKFMSRHRTGDVDALVAAINAANKTVCISVMDYAPTSFYNRPNFYWPLIDDAIRSAAFNRNVKVRMLVSHWNHTNSKIIPQFLHSLNEVKNVQVRWFVVPDLPFNPQVPYTRVNHAKYMVTEQQSYVGTSNWSWDYFTNTGGLSYNIFNANFTSQLQSIFDRDWNSEYTYPINRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWM1</accession>
    <name>A0A8J4UWM1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000987</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000022">
        <property type="protein sequence ID" value="KAF2077671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWM1"/>
    <dbReference type="OrthoDB" id="70250at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007720">
        <property type="entry name" value="PigQ/GPI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21329:SF3">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21329">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT Q-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05024">
        <property type="entry name" value="Gpi1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="442"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="523"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="556"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="600"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="637"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="315"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="711"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="218"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="256"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="329"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="719"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="736"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="746"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="777"/>
            <end position="786"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="870" mass="97347" checksum="186F5D2415D09D24" modified="2022-05-25" version="1">MDGSGNGSGGMASPASNTANAQAQPQSQNTGNPSFKPYNKIFWPNRCIDQKSGYLVGLNTRSFVACVVDIVTDVPLEELDIILNKMSFDRRMLDCPLGHSPRILGEWIGADKASLPLPPNLHELDMWISMERTESGDPSLRKIYFNEYKIQTSSQIVLYDDTPPYYYTSIPIRFESMLSIVSNSLKTKSSLSQSASKLTKTNNSVFSPTTVNNTIKPSSSSSSSGSSDLETTFKQINCSHDIKTILRETITKHNSIKQQRTQQKIKQLASKASGSSSSNNSSINTDSSDTDSEPSPSFFSKTLFTLKFRNKRKDINSTASNSPSITTTSASSNKLNSSSHSNNRKPKRIIDYILSPIYLIVTIVQFVFDLIEKILNIPTPFGPQLKHLTTFGTHLDNRVFQIKQLSKQWNHLKEKKYWVSNNDDRSSFIEFHNSAWLIFNDILLGIGACILLNVFSHFVLLNFSRLNHLLNNNMLKSLILWLMGWPGGFKLNENLDNFFGRLVLYYIDKWNLITTTISPHGEIFIKVICLCGVLGLSFLISVGIDLFNIFTLHISVFYSVAARFYLLQLLLLKSLWNLFRGVKYNPLRKRTDHCDFDIYQLLLGTLLFTLIFFLFPTTAIYYLFFALFKSGVSMVKAIFFLLLHFLNTFPLFNLLVYFIDKKYLPGGVVFSLKEKKDIITVNSSSSNSGNNSSNDLSPKLGKLAKTPSIEVTPIPSPPTLSSLPIHTLPSTSTPTNIQHNRNQSVPLLPSLGNILSSGGNGSQESKLPLSAKDSLHTFTPTSTTTPNTPPIGSQHRRNQSLSSAASPSIPRMPATQQLEISYFILQIKSASPINFFTPTIHMIQQVVQMYHPARLIKSFFMGVEIGKINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWP1</accession>
    <name>A0A8J4UWP1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MACPF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000978</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000021">
        <property type="protein sequence ID" value="KAF2077731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="21110at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF5">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MACPF" evidence="2">
        <location>
            <begin position="1512"/>
            <end position="1852"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1607"/>
            <end position="1631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1748"/>
            <end position="1770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1615"/>
            <end position="1628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="2278" mass="255784" checksum="77B08DC50958B56E" modified="2022-05-25" version="1">MNKTFIYLGFFIYLFVGAQLIFGAPQLKSYKYIDEDIIYNTDSCDNLVYHLSIYDNNVLLPNNIVLSTPSSVQSITVYTIKSEAPNYIFAVTAQALFSPDIGILTLSATNEIGENSIMSLDNTPVVCQHPNTTLDQSMFSFSKPNFKNNIISFDVKVDKTKTRYSPTSSSVSTNITSDYSTSISVYSTLLTGDKYVYRVSVIPSITKLMASQTFTVKFTFHAFPSTLEIELENPFNLRELSQTAPPTFDVSYPPLAPIQFSETDYDLNSVVIMVTNHASSNRFLAYTSKNRNTILAYPDRPIEFIPTFSGYDPILKDNTFLTAIIDLDDNITEEKKIIDVYYLLEKDGQISLENGSLHFNFTGRNKLAWLPSVSTVSDSTLNMGLVKMMLGDVPMNRNPIIQDFSGKSAPYPFPYGYSGSGTDGLVYSVSQIAPISSSRRYYGISSDRQHSYYSGSYMSPANTINDISAPVVKSYNIKKYTSNIIVLTLEVTDQGSGVREFYCSTPSVTYNAIDTLVSGTLNNGIFELVFDLSKDLVFYPNLEIQIYDFFNNFNTLPYLSFDVNESKQLVGLITVSDFTYLKFNHESIDTTFGRKKSSLFFRVNDLPLDYVPQLAIIESSLEFTNSEQQYQIFQGKYHQGLEMFVIEFEVPSKLYDRNVDYMLFAPTKFDGQTLETKFGSDARLYLTSEYFDNYPPIIRDFTFTASHQVEIDVDQEIEIGWDFRIIDQSNGFSYGTFEITSDKDGSPFVFNITSLDRIKGNDFDGIYPVTFKVYGNTVDQTFSISAVSLYDKNGRVSTYPSWEYPTRISPLYLLSKDHSIKVVCKNVVSVNKLAPSLKPVDGFSHSFNEADRVITFTLKVIDNGGKGFSPFSKHNPIVYIDGIYEHIKVQTEVCSSCANTASLVTYTASVQLSYNLIISGCSFSVFGIVDRHLNFYGANSIELKQLGFLSTFKTSNVAIFPVISSLSSISDMGGDITIYGQRFPAAAKLYTQSDAGAFVERQIKFISSTMIIATMPSSKTDIQIKVLYSANTYTNTLTVSPTKSSKKKFVYVSPTHECVSGCGTHTNPYKTIKSALLNSDPFSETIILLPGIYSGLENNEILISQYREIFSLAGPETTIIDCEGYTYAIHAQNAKRLVLSGITFRNCVNDQGGALHLYKTPTKLVNVDFVNNNASNGGAIFVYGQELAITDSIFYKNKVINHGAAIYSDLAIVKINGDLTYFAPIVESSNTDMDGRPKDILCKNSSIKVDTSVSMYKVDFECLDGCDSAYSNKPLCLNTKSNKKGKSICDTESCLTRPDECSCLFSGLVLETYAQDCDILNVTKCKSLNKEPSQQVELTNHLGGFDVVVNRFYGYISVSESKYLTFLFTGSNYGFIFKINAVQQDSMYQNTRFNQTKTIYLTHKHVNFIEIIVFSTVSQGSQRSFRMTPLLSINDDMFYSNLICGDKVKDKLETIQFYENPIINDDDEDDNIPNIFYCAADLPYPSLGSEPECGDGICNDVKPCFKDCFNVLTKSCPTRTVPDGHIAPGFFFSSDTLGDLISNQFMWKLPGSDHLTFGVDIVSGEEASAPIFQFDYCQHIAQTVMEDIYRGNVYLLPDQINARPLPECSYSSSTEMHESSSEISKSMEESSSQSYEASASGSYMGSGASLSAAFSLEKSSKESNRIALHPNLLKSLSEVEDVEGVVELVKMYGTHYYISTYLGGKLSQLTIAKSSDATSEKESSYSSSAAASLSASVNSPSFSVSASVSGSLDSSNDENEAKSNSESSTTSRILTYGGVPAAFSPAQDGESSPTYSDWSSTIDLLPVPIDYRLAPIRDLISDTWFSKNNNETNLQQLWLSAEDIYYAMNNFDPGFSTDSFSIIFELDVIDGSTSFTTNSFPVLDVKYWINQKVGNVTKKVEKNFFVDLDFVHIDNNGSQFYHLYSNNLNEAMGTCGEYTAFDRDYSNLWSTKKKSCDYGSIANMKYPIRLEFEAPDFFLSVDRPEIKLTFNDPRVKIKNDNPIKIISWKRNQAILVDSNGVIDNYGKPESEQEYYATTAFESRWAWHTSKHLAKSAFSNGRNTGIDCSLIPDSCSDLVRFKFDEPTQYSNSDTRPVPKDRTYTNGFFRDFNQNSPIINWYSFKGPIDEKFKKGILQVDHDSKIGTLTRINWVKAYYPNSENTWFFDIKGYHIKDFQEPKYHSKEFIEVNFDKYTPQDRFSPFKWYQLYPLNDAATKESIKTRQLYFYHNYNFNIKGVYSYQLDGNYDANKAPTYDYVLLQGIENSVFMDNGNSYSFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UX11</accession>
    <name>A0A8J4UX11_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008337</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000504">
        <property type="protein sequence ID" value="KAF2070350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UX11"/>
    <dbReference type="OrthoDB" id="48988at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19101">
        <property type="entry name" value="AKR_unchar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43147:SF2">
        <property type="entry name" value="NADP-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43147">
        <property type="entry name" value="PROTEIN TAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="1">
        <location>
            <begin position="16"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="338" mass="38892" checksum="CC8229812D597BB5" modified="2022-05-25" version="1">MEYTTLKSDPSFTFPKIINGLWSMSGGHGKINIKHSFNDMIEYVNNGLYTFDMADHYGPAEDIYGELVVQQQHQQSTSQGTLVNRPVQGFTKWFPRPGHMTLENVRTGIQKSLNRMKLERIDLLQFHWWDYDDHRYIDALDSLKILKDEGKINHIGLTNFDTNTMQKIVEHGIEIVTNQVSFSIIDRRAEKKMLSFCKDKDIYILAYGVCLGGLLSEKFLGVPEPSQTSLNTWSLTKYKDFVNRWGGWNLFQQLLEVLKQIGIKHSVSLTLVAIKYVAQKEMVGAVVIGCRYGIHQHFQENKQLFSFTLDSEDLDSIDKICLKGDCMLGWNDCGDEFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UX16</accession>
    <name>A0A8J4UX16_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_008757</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000572">
        <property type="protein sequence ID" value="KAF2069928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UX16"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF140">
        <property type="entry name" value="INACTIVE PROTEIN TYROSINE KINASE PTKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="162"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="67"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="92"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2069928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="458" mass="52562" checksum="BDAA8E11B157EC44" modified="2022-05-25" version="1">MNHFEKRLILLKGDLKVQFMQLIKSISSKKDQILVQDLIDSRLRNLQNMYIKNQSLTLNQLLDFIRDDDDDDDDDDDDDDDDDQDKDSQEKEGDDEKENEDEQEKEEKEKDNRVVIKSIPNKPIVEKEIESIGNQIESMEVQEQQPKAPILPIDREEFDLMFSASNKIGEGGHCVLYKYDKDRVIKVLKSQTRFTVELENEIDILESLDHCNIVKIEKGSKEERCFVMEYIDGDSMEKYPQQQQNDINSSPSSSLSSFGNPHPLKLIESIQDIVSAMIYLHNKSIIHLDLKPSNIIKSSKGELKLCDFGISRILSSSSAAVGKSSTSAPQQQINCSFRYSSPEIFNSDAVIDKSSDVFSFGILLWELLCWRLPYGILGRDEIISISSDEQREKYLSLKGLPQGIQDLIRLCTRQNPQIRPSFIDIQKRLTDILLSEIPCQSGKDFWLKICSTINVCYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UY25</accession>
    <name>A0A8J4UY25_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_007354</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="AJWJ01000387">
        <property type="protein sequence ID" value="KAF2071323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UY25"/>
    <dbReference type="OrthoDB" id="17594at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050940">
        <property type="entry name" value="Actin_reg-Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46485:SF5">
        <property type="entry name" value="CENTER DIVIDER, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46485">
        <property type="entry name" value="LIM DOMAIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="18"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="12">
        <location>
            <begin position="594"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="11">
        <location>
            <begin position="852"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="171"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="353"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="683"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="973"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1059"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="366"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="683"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="980"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1059"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1090"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1132"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2071323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1142" mass="129239" checksum="18D231DBE7B768F8" modified="2022-05-25" version="1">MVGGCPNSSLWNVEYEELVFKEVIGKGNFGCVYRGTYLGVEVAIKQIPTFDDPDYCKYTEREVRALRYIRHPFVVHFFGVCKHSSGFYLVTEFIEGLDLRRFVKISSLPSATPPTWESRVMIALDIAKTLLFLHSKGILHRDLKSKNVLLDIPRNVTKICDFGFARMGSQYSNGDDSSSSEDEDESDHHASAGGADQVYKRNGNGKPASYRLRRMSICGTPSFMAPEILLQQKYDWSVDVFSFGVVLAELLTLKRPGKDFWIRNQNNGFDISLDELKANITGGTSHPCPQPFLELCIRCCAYLGINRPKFSDIIQSLEDLKLSMNPNPMNQRPHFLNTLSRTNSLLLLSPPAKYQQSPRQLDDKNNQPTNNNNNNANNNNQHQKNNIQTPINSSINSTSKNLRFLDPYLEPICIFKEKKRQSVKHSPANLSIPLGSPTSLDSYAIDGVLKLLNPNQFPNGWKDFGINSHTTNIWLLFETKDSLNKKSTPSIEMTCVKLIKIKKQCLYIYSTDPNLYDGIGIVNIKPYQIEYPHLPSISISSGGRGLDPIQDYDVKDITKIQSVVRGWLVRRRFKVFQNNWSLSSTSTLMSNKRTWMLMFNQLIDNEVVYKNQLDNVIRSYLLPVQSKFRINKPLINYKEIASIFSNIEAIFEISNEFLKLITLVSNSPYFILNVGTNLNLDSPLNNNNNNSNANDDDHNPSSPSDNMPTEIQSIAQFIIKNINQIKNQYGIYAYNFKYAMNILNWCRLNPEFGKFLETVRMSLNHNTSRLDEENDLQSLLSLPINKVQKYLLVFEKLVQITPITHTEYSDIKNAYTLIKDTSNAIQTQLEMSFEHSHLMSVDIMLLRKDNVPIAQNGRWFVRQSAFEDIPSSRQYYLFLLSDVCLVTKPVKSKSKDSTNNKKFYYQLRRVVNLKDDNVSMRPNNDQPNSVIFTTPSKVYKLAATSEEEAMDWINDFERTTLLVYRNNVLDGSNDEGNGVGKINNNNEEINPNIPQPTLSSSLEHKGFIKRFRQSITSGSTPDRKSTSLNILTSNQSAINPSTGNSSISLSHSGSQLKSVLSSPTINYSPTSTTTTTTTASSSTGNVGSSLNQSSTENFVIPTTPSEKKKVKTSFTDKLKRLSHTFTSSPVGESKKSKEISKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYU0</accession>
    <name>A0A8J4UYU0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006676</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000318">
        <property type="protein sequence ID" value="KAF2072013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYU0"/>
    <dbReference type="OrthoDB" id="15710at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="424"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="544" mass="62681" checksum="C0C624A19A511789" modified="2022-05-25" version="1">MDSSIDNNNNNNSSNSKYDNDIEKEDHDNIIDHKPFLSSLLKYFKPQIQLQIHPKEKNTLQDINNFKDVNNNDDQDNEEDDDDDNDDDDDNDDNDDDDNDDQENENDQGETLDHDNRTRNNNSGGSSSMHHDDDDHQVVHSGLLKDMFDYFKKKSMSSTTLHQQQQQQEKDQKDDNSSSNNDNIELTPTAVLLSSGYFKFISSWFENESKDFEEKLIEINKITEDETIQDDTKAFEKIVEVMGKKRFYEILFLLGSASIASFALRTLYFGKTIRAVEIPDGNLTTLVLRFNNLNRLKKQLLQNQPALFPGVELTSDDIKVNTFIDPHFTEPMLTNKHVRMLDDGGYIVWTKGDFEIPRTAIALKAMVKDRDTFMRYSANAELDKIIRLISLLGPPLSICPITTKVYNINNYNNVSNNIFNFTNNNNNNNNNNNDHSNSSTNNNSNNNNNNNIIKIISSKYKIIVQSIKTKLLEYGILTPTIQDIIAHDPNPPLSMKERDDLIFLLLSDKVQNEKIVHLYHKHDVLEFKELVRKLVVVEHKPLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYU3</accession>
    <name>A0A8J4UYU3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Signal recognition particle 19 kDa subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_006662</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000317">
        <property type="protein sequence ID" value="KAF2072028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYU3"/>
    <dbReference type="OrthoDB" id="2190947at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006617">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.30">
        <property type="entry name" value="Signal recognition particle, SRP19-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002778">
        <property type="entry name" value="Signal_recog_particle_SRP19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036521">
        <property type="entry name" value="SRP19-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17453">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 19 KD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17453:SF0">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 19 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01922">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69695">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0733">Signal recognition particle</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="131"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="162"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="176"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2072028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="20449" checksum="A624BC9966761710" modified="2022-05-25" version="1">MSKKGGNAPPPQQPQAPGAGVKVVNTAQIPGFKKWIIVYPRHLNSEFSREDGRKLSKENAVKNPSLEELAKASSELGLQVIIETSKGYPKDFFQRGRVRINMLRENGTPYVNDIDNKTKLLIKMAAHIKNTQPNRPEPFNPLSLLPAVQATSARKEEKKQATTTTTTTTSSSSSKGGKKKKNVNVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYY1</accession>
    <name>A0A8J4UYY1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">mRNA guanylyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_004924</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 5'-end diphospho-ribonucleoside in mRNA + GTP + H(+) = a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:67012"/>
            <dbReference type="Rhea" id="RHEA-COMP:17165"/>
            <dbReference type="Rhea" id="RHEA-COMP:17166"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:167616"/>
            <dbReference type="ChEBI" id="CHEBI:167617"/>
            <dbReference type="EC" id="2.7.7.50"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67013"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 5'-end triphospho-ribonucleoside in mRNA + H2O = a 5'-end diphospho-ribonucleoside in mRNA + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67004"/>
            <dbReference type="Rhea" id="RHEA-COMP:17164"/>
            <dbReference type="Rhea" id="RHEA-COMP:17165"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:167616"/>
            <dbReference type="ChEBI" id="CHEBI:167618"/>
            <dbReference type="EC" id="3.6.1.74"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:67005"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000184">
        <property type="protein sequence ID" value="KAF2073755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYY1"/>
    <dbReference type="OrthoDB" id="200924at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140818">
        <property type="term" value="F:mRNA 5'-triphosphate monophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004484">
        <property type="term" value="F:mRNA guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004651">
        <property type="term" value="F:polynucleotide 5'-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07895">
        <property type="entry name" value="Adenylation_mRNA_capping"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07470">
        <property type="entry name" value="CYTH-like_mRNA_RTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.100.10">
        <property type="entry name" value="mRNA triphosphatase Cet1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033469">
        <property type="entry name" value="CYTH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001339">
        <property type="entry name" value="mRNA_cap_enzyme_adenylation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013846">
        <property type="entry name" value="mRNA_cap_enzyme_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051029">
        <property type="entry name" value="mRNA_Capping_Enz/RNA_Phosphat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004206">
        <property type="entry name" value="mRNA_triPase_Cet1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037009">
        <property type="entry name" value="mRNA_triPase_Cet1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367">
        <property type="entry name" value="MRNA-CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367:SF17">
        <property type="entry name" value="MRNA-CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03919">
        <property type="entry name" value="mRNA_cap_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01331">
        <property type="entry name" value="mRNA_cap_enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02940">
        <property type="entry name" value="mRNA_triPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55154">
        <property type="entry name" value="CYTH-like phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="mRNA triphosphatase Cet1-like" evidence="14">
        <location>
            <begin position="132"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="mRNA capping enzyme adenylation" evidence="13">
        <location>
            <begin position="306"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="mRNA capping enzyme C-terminal" evidence="15">
        <location>
            <begin position="497"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2073755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="630" mass="73809" checksum="6CCA1F36D8BB787B" modified="2022-05-25" version="1">MNSNIAVKRTSNHVDGGAVQKKSKPDNHDPLDTVLGEMNSYQHGKTNHHQQSKSHSFSTENLLNRILDMTKVDSSNVDNLEVEGRIGLFRATEGGNFKPGMIKEDFDTLLATLSQKRSPKFLKETDYIFSDYRITYDEENKRCLRKESKTDRTTFDQSTNLVYDMRVSLSIEDSMPPPTHLPLDYSMKRDKSRFTFHERDWKVDLTHVVVYPKGGLPEELFEVEIELYTSSIKQNTDKQSLTNLLNEFIQYVRFLITSIQKGGLSFADISLEKVENVQEIYELRERLYSYLPKTEKKREMFPGAMPVNFSKKNFLEVQSNRYFASEKTDGIRYMILITNNRSFLIDRHFDFYSIKDYDVLHTLFGRGTLIDGEMVRNLNTHKPVYLIFDVLAVNNEAFYHVNLSQRLGVIGQIISQLRQEIEHISTPFEILGKVFHPKEKIHEVFNCIKDRGNGERTFIDKRRNHFTDGVILTPDVPYQTFTNSNLFKWKYLDKWTIDFKINFRQNRFFLSCTGTQNTEIDCREANFSPEDMEKLRIELKRARDPTIVIAECSFQPKIGKWKFHQTRPDKKKGNFITIVMDTMESIAENLSHEELKYRIPLKPENDNWDDEMSRLKSSMFSSMKPTQSYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZR0</accession>
    <name>A0A8J4UZR0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Choline transporter-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_005271</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Choline transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the CTL (choline transporter-like) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000205">
        <property type="protein sequence ID" value="KAF2073423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZR0"/>
    <dbReference type="OrthoDB" id="420519at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007603">
        <property type="entry name" value="Choline_transptr-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE (SLC FAMILY 44)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385:SF101">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04515">
        <property type="entry name" value="Choline_transpo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="275"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="320"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="375"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="427"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="490"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="527"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="555"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2073423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="624" mass="70706" checksum="F47D38F963EFE430" modified="2022-05-25" version="1">MTSKEDDDHNEISKEVIKNKGVYHKRKCQDCFFLLLFIAFWGGMIAIASIGVRTGAPERLVSGTDSYGNICGVNNIAEGKLRANQSRDLSDEKYVYFIYYIQLNSYRVICVNECPTETYINPSNETELICDYDQTPSMENLFPNGTCAGKIASSPFANRCLPSLLLNGTEKVVDKVYQFINNQITSDTSVKVLQDIVLSWRYLIYGALIAMGLGLVWIFLLRFFAGLITWATVLAALACLGLLTAQVYFQWKEAERTYDAIPPSQRLNMQYDNVLALKVIFIILCVISGIFALILLALFDRIRIAIRIIKESSKAIGIMPSIFFFPIFIFIILCAFILYWVYIGVYLATSGDPTYDDDGNFQGYQDSTTMRNMQIYHFFGLLWTIAFILAVNQCTIAGAISSWYWVQSKKDVPFFPVWASLWRVFRYHLGSLALGSLILAVVQFIRWILRFLEKKFKGKEAFLARFVVKCLNCLFGCFERFIKFIDKNAYIMISIYGYSFCAGAKRGFTLILSNILRVAAVNMVGSFLMFLGRVFITAATVGISLYMLQKNTDLTFYVIPAILIGFIAFAISTGFMSVYDMAIDTILLCFCEDSERNDGSPERPYYMSKSLRKFADGKGSSKCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0H8</accession>
    <name>A0A8J4V0H8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000089">
        <property type="protein sequence ID" value="KAF2075690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0H8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2075690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="101" mass="11257" checksum="51E1A49002BF9960" modified="2022-05-25" version="1">MIYFYNQVTANRAFYVSVNKWGDAGETTPYKVNRNTSETWDRSDTRGFIVNIGNTSDVDDVSNKAFLVFDGSTVFTKNNGIFLTSGGITQKLMDLPPVSTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0R7</accession>
    <name>A0A8J4V0R7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_002547</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000071">
        <property type="protein sequence ID" value="KAF2076140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0R7"/>
    <dbReference type="OrthoDB" id="290445at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31318">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31318:SF1">
        <property type="entry name" value="POLYMORPHIC MEMBRANE PROTEIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035246621" description="Transmembrane protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="904"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="468"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="936"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="479"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="936"/>
            <end position="948"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2076140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="955" mass="103955" checksum="8F46A1BE584C2B9E" modified="2022-05-25" version="1">MYKTILFLLLALLNLYGIYSSSDHVPILYVNPQSKNTKIDCGKSITSSCNSITSAVNSFINNSTVYNQDEILPPLIIELMPGEYYPSNTTFLQCLSIPLYKFNITIQGYKNQEIKISGKHLIGEGPFFWFEPSSFDKFKKIPTLVNVRNIEFQDFPLPIVNSNLFAPLAISFMNVNFTNSFINVPYPDQLFIIFSSSKNQSVLQFHNCMFSNNNNILLSQGAYVQFINTTITNTKSLYPILSFPYSYPSIPNTLVFENCLFQNNQATNHGLIYSIQGKIILENSILKNNVADSIINCFNSIISSSSTSFISNIAIKDNIINIKNNSLLEVNKCIFSLNQGSLGTIGSIDSDVFIGESTFKNDFCKQSIISQSGGVLKIQESSFFFNLIDVANLYHFENVFQLLENTRVLMNNTSVVIKEHHESSVVSVIDCKSSFIKFTNTTITSKTPLPSLYCQTCDIENQDNNDGRSIYSCQKPNQSSSDASNSNSRNDNHSHSSHSGHSSNNHPKKSSTKHMAFAANTVLYVSPAGKTAAQCGATIATACPTIKDAVSVFAGGKGPLIVKLINGQTYGATDLSGIQLYGLDITIQPNDENSTITITGDQSTTSFFNIANNGADATSVTVDSIVFDKFSNAILTATNIKSQLIASFNDCVFSGATSKSDLIQVSSSAPQGVVVSVSLSKVQITNNDASVATISSAALNILDSNIESNTAVSADLITLTSSQATLINSNVSSNVANVANLISSSITTNNTYFIANIGGSISTVFNSFESTVTINQCSLLNNVGIVINSQNSSTLVINNSVFDYDYSASLSNAPNDMLNLTNSYAQIDNSSFSITGQKISDLITVINCETKSDIVFNHMPVAIDLGYEVPALCISRTCNMTNTNHTADPFYCVVAMDPKVRNKAISFIIGLSIMGAFSLVIVGLICARREILNRKDAKEDQDEQERQGLLKGVNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V139</accession>
    <name>A0A8J4V139_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ras-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008613</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000548">
        <property type="protein sequence ID" value="KAF2070067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V139"/>
    <dbReference type="OrthoDB" id="14138at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07307">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF60">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN RASKOL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GAP" evidence="3">
        <location>
            <begin position="345"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="631" mass="70838" checksum="3A3A4ECF253133B7" modified="2022-05-25" version="1">MSGLSVSGSGPAPTNTSPNTTLSSSPGTAHSSGTLSRKSMSPYKVQAAPKNVEWGLLGRTVGVAKTSVLQKMGKQETTQADPQLVIDNVKVKVLKVLYEETIRLSKKMVNKSQKYNNSVPPLKEAYQEIANYYTTEQFTGKAIEAISNLHVEISSLREKQQTSINNRFILPAETFQTGTINVAREAKDRFRKSRLEYDSALNKLKIVQQANNIDLKQLYICQSHYAKYRLRYLHRQYEAQTKLSDTVQRHGFEFLVQSINLLATLHDLYSNVCTLLNNNEPFINEMREYSANQKRIFAEQKQDNQLRLYEISREKEDTKYNELVEIFSSPDLAVVNAICLSAGSEQDIILEDLVRILDAHKQTLPIIKLGISKEVSSTSSAATLFRGNSTATKLMTAFTRMTGRPYLISTLKPIIDKIVKDPSGYELDPEKTSDTSGNVERLTQICQDFLDAIYSSISNCPLPFREMANHLQKEVVKVFPESKHTSVGGFFFLRFFCPCILSPDANGVTDSASLTMEARRALILVSKTLQNVANGIVFGTKETYMKDMNSFVEKNIDTCKEFLDKIAEMPQATDYQPLSSIAEVKSNQLPSIHKLLIKNLEKIFKTLTQYNQQSCTFQLVSALGKLGDVEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1F9</accession>
    <name>A0A8J4V1F9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">L-type lectin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_010575</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001208">
        <property type="protein sequence ID" value="KAF2068099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1F9"/>
    <dbReference type="OrthoDB" id="10265193at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005537">
        <property type="term" value="F:D-mannose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051136">
        <property type="entry name" value="Intracellular_Lectin-GPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005052">
        <property type="entry name" value="Lectin_leg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223:SF28">
        <property type="entry name" value="LECTIN, MANNOSE BINDING 1 LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223">
        <property type="entry name" value="VESICULAR MANNOSE-BINDING LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03388">
        <property type="entry name" value="Lectin_leg-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51328">
        <property type="entry name" value="L_LECTIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035233513" description="L-type lectin-like domain-containing protein" evidence="8">
        <location>
            <begin position="28"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="536"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="L-type lectin-like" evidence="9">
        <location>
            <begin position="40"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="266"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="279"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="345"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2068099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="568" mass="64949" checksum="77E417A1FA54C253" modified="2022-05-25" version="1">MKSSNIFNIIYISLLILLTINAFVVSAYPSYGGGLSSGTVRREDRHSFRSPYLFTGSGIQYWDIFGNTIVTEEYIRLTSDTKSISGSIWNNEPMEFPWWEVVLEFRIGGNGKVGADGLAFWLVDKKHNENSEDLSVYGSKNLWKGLAVIFDTFDNDGNGDHPLISVFYNDGNQFYETAKDGANMKLGSCTSRYRNDKKNAKARIKYLHGQLSVQIDPTSSGTFENCVSDIRLDIPIRYHLGLSAATGGLTDNHDVYSLDTFSLDAGNNPYQRHHNPSVTQAPQQQQYNNNEQQQQGQQQGQQQQQQGQQPRQTQYEQQQQTAPPQEPKRYDEDEEIFKNLGQYGNDKNTNNNADNNNNNNNNNNNNNNNNNKYEDQALNGLGESEKFTYLANKVESLKDTIHQLHIVLDDPDYKKSFDGLRDQVNEISKGIAALANLVVSKRDFDNFKTQYDSKQTNFIKDFYEFKQTMQDLKATLNSRFTNQDNDISRNIESLNQNVNTLRKSLDMTTRDTSKIASLLQGNANEIASTIEKHSSFGFWTYFLFVQAIFIFGFIFWRKYKDENNKKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1R9</accession>
    <name>A0A8J4V1R9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002976</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000088">
        <property type="protein sequence ID" value="KAF2075733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1R9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="227" mass="27256" checksum="F94B1043F7EC6D0A" modified="2022-05-25" version="1">MLFKSREKELEESLREKDVLIRDLTNTLTCKAKRQRLKKEIIELKSLLATQETQVQQWQKESKRKNKENKSAVKEIRAKENNRILQLQSQLLDANKEKEEIRVKENNRVLQLQSQLDKLNKDKQDIRVKDTIILELESQLEKSNRENRDIRSILNSRDTTIQDQLNQIAKLEKQCRETGKEKDQIDSNYRQSKIREQEGKKNKRKRNWKEYGMKSKITLKHIFLVQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3U2</accession>
    <name>A0A8J4V3U2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008526</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000533">
        <property type="protein sequence ID" value="KAF2070154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="19017at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00065">
        <property type="entry name" value="FYVE_like_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057555">
        <property type="entry name" value="SAC9_GBDL_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057553">
        <property type="entry name" value="SAC9_GBDL_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46817">
        <property type="entry name" value="PHOSPHOINOSITIDE PHOSPHATASE SAC9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46817:SF1">
        <property type="entry name" value="SAC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24790">
        <property type="entry name" value="SAC9_GBDL_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24789">
        <property type="entry name" value="SAC9_GBDL_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC" evidence="2">
        <location>
            <begin position="279"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="506"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="640"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1118"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="509"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="531"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1122"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1135"/>
            <end position="1144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1744" mass="199151" checksum="4273A09291283A3B" modified="2022-05-25" version="1">MDTHPVGSNSNHHHNNHNNYNNHNNHHNHHHHNSHGSNQQQQQQQQQQQQQQPTPNKFTFHDIYETSHGDVFIISSLSNRKDTQVIRIEGRCGMMLFEGLPGVDLFPNHRQAATYLQKKYILKSKMRVFAILGYIISEKSSHLLVATKIRNEGDLLGIHTINTIIETAWIKIELSYLSSFQSNEAQQMDEAWVTTLVDEKSIDSSFSSSSTPINNNSSGNNNNSSSSSSNSNSGSNSNLGSCYINTEEVLPTISYPHQTGTGQKATTNESTAKTIIKDLEMFPLEGTHFYCETFDLTNYFPSNQTTPYQYCSEFTWNEWLRKPFENSSLSHLCVVLLQGYTLIKPIPTLNISIAYIMKRSKLNPGTHFYAPGINENSSPANEYECETIMWKVIKDVGRGANKGSKYFHHHRYHHDEDYDKEHDHHGDEDYIDEFHHPDDVYVKWTNHIIRRGTVPLWWRAFSKNQGNEIELFLKDKGTENPNPFEKTEQYYQNLLNRYKPFYQYNKVKNNSNNNNNNNNNNGGSNNTNSSVHQNLHSSNTNLDNIGVPEIQLINLLKQDNDKQEYYLSKLYQQSCEYVMSMLNVDLSIHQFDWTNTLKNNNNSLGKTVSMLWDNTKQSLESSNYSSGLIKISNSDDSSKIPLNNNINMNNNNNNNNNNSNSNNIDKQSNNNNNNNKKLIFLKYNPQKEIIRYSCLDSLERVNLAIFFNSFQIITTMGQHLGISQLSELIGFGQIPMDYFANTFKNLNLLSPLTEIFISSADISSILYQNNTSQQSTAMKEFLVNNSGGASLVSHHHLLSSQRKYQSTHNDEYRSYQYLLLLGNCPTGSVGDCQRLFPFNSSIGKDISPYWISSAPSSCIKYLPTLLKNTSTPSLNPSILLRDIDDDFCWIAPKEDHSAEVTIFLGQPSIVGEICLTMAHGTNTDTYPDTMDVLIGNYLNETTMVLQDIYIPRCTTGTKLSFVLPKGPWESFMNTPLSNIVNSGGVSLDQYFTRIVKIVFSGKHTPLTIGKIEVFGYNSNSFFHNSHLQININPTYRNSFERTNANIGNGGSANYSSITYKNDIIHLMEKQKDLYQQRNLCNENRVVNTFIDYQVDNILNILTSNKDKKNSFSIFEKFKNNNDGDDSDDDQDQNDDNSNQYNNNQDNNIIQQQKHYQQQQNNLSKSPSLVGSPEIISTSDEILLINDYESKLDLDQSIETIQMNNNKNQNDCSPLKQYETLVKRLYSLNNQGKNTLFLEALELEWKRIQLKITPMERDLLLKQLAIPSFIFNPSRFIFERDEKLESSIRKNSKFKSSICQHPQCGKPLGSLTFLDKLRTQQCSYCYKKFCSSCISSTPMKIFEFSTAKPFSICKICAVLIQKQESLFQKISTWNRSFYLEKTKFSLSFYQKIMADQYFSHNHVNPTLYNISSICHTYDSLNQALIPLCEYPKAAILNSIETHKSSPPIEIVLLPPTTLPLNMYWYAPDNVNSVELDIVLAYDSLVYSISMITDSLGYYSFDLPSVEIKVCKAFSDEYQNIGPWVLHPNGWSSNPNLIIQPQSCVSFILPEPIQARTISLKFTLPALPTFLKYDNGHRPFLHLGRIAVHGIFSDHLTDIPTFNSIQMDSTDKDLYENTLYSNSNIALKRNPIKTNITVKSPKVVHINLSNPNSIRGFSLIINHDDFEGIKSQVKSITISTISVNQKNELLSCQFLTQVLIPKCRQNSTLYFDFPCPLPPLTQFIVCEFLSNYGGKITSLPKQIALY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4L1</accession>
    <name>A0A8J4V4L1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000191">
        <property type="protein sequence ID" value="KAF2073652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4L1"/>
    <dbReference type="OrthoDB" id="7600006at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="493" mass="55745" checksum="8F2AA1255F05464E" modified="2022-05-25" version="1">MQTVNIDILFFKVFRDVYLESLIKNQLYGVKCNKKIHVKSIKDAEANYQYLLKVPSFIQIVLEIDNSGDESDRYETFLKSKQRHVVNYLVLHYKITNKHNASLLDYENDNHMLLNEINCLKVSSAIYCINYLPRYTNISKLVLDIIDLPLEAGTIPNTVTDLTINSDYSYPLHIGVIPSSVINLSLRLFNQPLLPGQIPFGVESLRFLEFNEPIKQGDLPESLTHLDLGYCFNRNITPNSLPSSLTHLEFKYTFNQPLAPHSLPPNLKILNLGSSFEQKINTHSIPKHVVKVVFGSFDQEILTPGIIPDSVEEVIFSSFNRVIVPGTIPKSTRVLKFGNYFSRPLEVDCLPPNIKLLEFGEGFNEPLKPNILPRYLRTLIFESCLTHDLMPNVLPLFLKTLSIGLAGDIAINVLPPTLTTLILKTFKGMIHPFSLPDSLETLKFSSSNCNFKSLENQNVINSGLKHLSLPKSILSLNICICIPPSGKTKVFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4W9</accession>
    <name>A0A8J4V4W9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_004704</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000171">
        <property type="protein sequence ID" value="KAF2073997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4W9"/>
    <dbReference type="OrthoDB" id="8061355at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031288">
        <property type="term" value="P:sorocarp morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000665">
        <property type="entry name" value="ABC transporter A family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF272">
        <property type="entry name" value="ABC TRANSPORTER A FAMILY MEMBER 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="144"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="231"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="300"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="369"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="471"/>
            <end position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2073997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="792" mass="89397" checksum="9C41CAF2E42AD04D" modified="2022-05-25" version="1">MPTKAINQTFDPYFETILPFYESHQGINTFIGQLNANGTNNQGFLGSIPQIPIIYNGTKGAKIVFTPSSQSFESNAAMTDTILGVKTQLVSQHRKSFKIKNLDQLPSFAVNFTQLDLPSLSLAYEISAEGQRMFQYKGYQVPGYIIYGMNLLLTGLLNSGLGLNIAVLSGAWELPYQSASPAIDIGSLLGGLFYPFALSFLLPLFVYSIVLEKQDKLRDLCLMMGLRMRNYWIVTYIFNFMLYLCAVIVVVGISAGFGFSVFTKGSAFAMVMFLFGWGNAQITFSFFLSTLFKRTRTASIFCYFLVIISVIVNLVLSAQLYLTTAPPVPYYFYPLFAFYRGFAIISTLCGIEECPTWSTYTWDFEPSKIILWLYIDTIFFLLVSLYLDQVLPREFGVPKHPLFFLQPIKNLFCSPSATDGEIIQQQDKKDETRLLIDSEEQQLLEEEIDEDVEFEKQKIINQQYNPNNTSIVINNLTKHYSGRPKPALDSLYLTIEKGETVALLGPNGAGKTTTISLLTGLYTPTSGNATVAGMSIKSNMDYIHRVVGVCNQFDILWEDLDCVETLLFYTRLKGIPKDQEMQVVEKTLKDVNLFEVRHRLAKELSGGMKRRLSIAISITGNSQIVFLDEPTTGLDIKTRRDLWVTINALKKDRCMILTSHSMEEVEVLSNRIGIMSQGKLKCLGDQQRLKTKFGDGYGLKLNIDPSYVEDLKTKSNPSNPLEFVQEFSADAKFVESYSNSFTYRLGKEIKLSDLFEFMLLNKDKHHVLEWGISQTSLEDVFLKIAANDNTVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V503</accession>
    <name>A0A8J4V503_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH La-type RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_004559</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000164">
        <property type="protein sequence ID" value="KAF2074117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V503"/>
    <dbReference type="OrthoDB" id="340227at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000339">
        <property type="term" value="F:RNA cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048255">
        <property type="term" value="P:mRNA stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07323">
        <property type="entry name" value="LAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006607">
        <property type="entry name" value="DM15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045180">
        <property type="entry name" value="La_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792:SF161">
        <property type="entry name" value="HTH LA-TYPE RNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
        <property type="entry name" value="LUPUS LA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21071">
        <property type="entry name" value="LARP1_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00684">
        <property type="entry name" value="DM15"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00715">
        <property type="entry name" value="LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="4">
        <location>
            <begin position="38"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="147"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="380"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="619"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="223"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="240"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="380"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="406"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="424"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="619"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="668"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2074117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="693" mass="80017" checksum="04DD90F0BC42540C" modified="2022-05-25" version="1">MVAETTSSDSLINSKKVYVGKNPHKVEHPPKPFVESTFVDIHDIKKSILNQIHYYFSVQNLCRDVFLRCNMDNDGWILISFISNFNRMRSFDINTIIESIKSSNIIELDSNLEKIRLISYEDRKLWILPQDTKNAFLESHQQQIEQQQLEQQKQAHNLSKSNEGWVTVTSKTQPKSILSPSINNGSNKKSSPSSSPPSNEEMNHKTEESVFDLSGDDSDEQDFFLKERRNFDERELPQSSEDEDTVFDDDQEHHHIDEQQEYQYEEYYSDHSEQDIIDEYEEQDDEEDDISSKLIIVTQSPYKKNKLNGKKYMTSEIASMINDGLYFYEQDLKRKKQIKPQNFNTVIVNDSSKLSNTAASKSVQDNPTRLYSPMIKKSIPIGSSSSSSSNKPNTLPPIGWVLGPKSESNTPSGSPQSNSGKNINTTPSTTTTTTKTSTNNNTTQPSTSMDEKLPYFQHPSYELLEENGFVQHKYNKYRSKCLKDRKRLGIGKSMEMNTLFRFWSHFLRTHFNAKIYQEFKDIAIEDSQTNFRYGLECLYRFYSYGLEKKYRQDIFEDFQQLVLDEYKSSKQTYGLEKFWAYLCYRKDSTKLEINPEMTVILDQFKSIQDFKRLEIKTNYSSNNSSKTPSNSYNNRSSSSYQKSSSSSSASTSFGGRRKSFGSSMAWDSRNTNNSKNTFGTSVGSQPSSRIHHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5E8</accession>
    <name>A0A8J4V5E8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">2-oxoisovalerate dehydrogenase subunit alpha</fullName>
            <ecNumber evidence="9">1.2.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_006841</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 3-methyl-2-oxobutanoate + H(+) = N(6)-[(R)-S(8)-2-methylpropanoyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:13457"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10497"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83142"/>
            <dbReference type="EC" id="1.2.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the BCKDHA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.4" evidence="9"/>
    <dbReference type="EMBL" id="AJWJ01000333">
        <property type="protein sequence ID" value="KAF2071849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5E8"/>
    <dbReference type="OrthoDB" id="3845at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003863">
        <property type="term" value="F:branched-chain 2-oxo acid dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009083">
        <property type="term" value="P:branched-chain amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000015">
        <property type="entry name" value="2-oxoisovalerate dehydrogenase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050771">
        <property type="entry name" value="Alpha-ketoacid_DH_E1_comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43380">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43380:SF1">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="10">
        <location>
            <begin position="104"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2071849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="444" mass="50555" checksum="CF17A38864295B4C" modified="2022-05-25" version="1">MNQSIKVLSNLVKSSNTSRSLINAGSRRSVLFKTLNTNNTVRNFSTKQSKIDKDDEKTFDYTDKLVVQKLETYIPCYTIMNQDGIVSKPEQDPNFSQEVVTKMYKTMVLLNTMDSILYDVQRQGRISFYMTSFGEEAIHIGSASALDMGDTIFAQYRETGVFLWRGFTVDDIVNQCCTNDLDDGKGRQMPMHFGSKKINLQTISSPLTTQLPQAVGSSYAQKLAGEKNCTIVYFGEGAASEGDFHAAMNFAATLSTPTIFFCRNNKWAISTPSKEQYKGDGIAGRGPNGYGIRTIRVDGNDIWAVHNVTKLARKIAVEEQVPVLIEAMTYRVGHHSTSDDSSRYRTVEEINYWKETKNPINRLKNYMVRKNWWTEDQEKAIVAESRQLVRESLMKAEKRKKPALSEIFNDVYSELTPNLIEQQKELEEHLKLYPDEYPIDQYAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5G4</accession>
    <name>A0A8J4V5G4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome production factor 2 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Ribosome biogenesis protein RPF2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_004197</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
            <location evidence="1 4">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the RPF2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000144">
        <property type="protein sequence ID" value="KAF2074492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5G4"/>
    <dbReference type="OrthoDB" id="407658at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039770">
        <property type="entry name" value="Rpf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12728">
        <property type="entry name" value="BRIX DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12728:SF0">
        <property type="entry name" value="RIBOSOME PRODUCTION FACTOR 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Brix" evidence="5">
        <location>
            <begin position="35"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2074492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="302" mass="34274" checksum="53CE8CA5D07ABE03" modified="2022-05-25" version="1">MATNQPTFVKRAPAKSIAAKKFLRDKEAKEIEFIKSAMFIRGQKTSQIIQDLQKDLYMLKKPDAVQYNKKNDIHPMESAESIEFFGEKSNSSLFTFGSHSKKRPHNLVMGRLFNNTVYDMIELGIENYKSILDLGGGGCGVGNKPAFIFNGSDFENKDDVKKIGNLFLDFFRGRLVEYINLTGLDHVIVLTSVEDKILFRHYNIVFRPSGTKIPKVELKDIGPSFDMNIRRTKVASADVEKLALKIHKSVQPDRVKNVETNVLGEKYGTVHKGRQDFSQIALRKNKALKKSRRPEMVGINSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5Z6</accession>
    <name>A0A8J4V5Z6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 14">Adenosine kinase</fullName>
            <shortName evidence="14">AK</shortName>
            <ecNumber evidence="4 14">2.7.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Adenosine 5'-phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_000098</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 14">
            <text>adenosine + ATP = AMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20824"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.20" evidence="4 14"/>
    <dbReference type="EMBL" id="AJWJ01000002">
        <property type="protein sequence ID" value="KAF2078598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5Z6"/>
    <dbReference type="OrthoDB" id="432447at2759"/>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00659"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004001">
        <property type="term" value="F:adenosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01168">
        <property type="entry name" value="adenosine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000076">
        <property type="entry name" value="Adenosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1110.10:FF:000001">
        <property type="entry name" value="Adenosine kinase a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001805">
        <property type="entry name" value="Adenokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45769">
        <property type="entry name" value="ADENOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45769:SF3">
        <property type="entry name" value="ADENOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00989">
        <property type="entry name" value="ADENOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 14" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="15">
        <location>
            <begin position="25"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601805-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU368116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2078598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="341" mass="37407" checksum="AD02536A9E7E29A8" modified="2022-05-25" version="1">MSSPKIFCIGNPLLDISAHVTTDFLTKYDLKLGNAILAEEKHMPIYEDLVKSFKVDYIPGGAAQNTARVAQWMLKDKQTVVYSGCVGKDENGKILREATESNGVLVKYLENSEVPTGACAVLINDKERSMCTNLGAANKFHISHLETEEMKAIINSVENFYMAGYFLTVSPDSAVLLGKHAAENNKPFLFGLAAPFIIDFFFTQVEQLLPYVDVVFANESEAATFGRKMNWGEDLAVIAEKLSVWNKVNTKRSRAVVFTQGPNATLVFQDGKLTQYKPIAVPSEEIVDLNAAGDSFCGGFLAAFSLGKDMAKCCEAGHYGAWEIIRQNGATLPTTPSKFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7C1</accession>
    <name>A0A8J4V7C1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pleckstrin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_002010</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000052">
        <property type="protein sequence ID" value="KAF2076697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7C1"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051092">
        <property type="entry name" value="FYVE_RhoGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673:SF87">
        <property type="entry name" value="DH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673">
        <property type="entry name" value="FACIOGENITAL DYSPLASIA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="3">
        <location>
            <begin position="94"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="376"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="444"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="572"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="640"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="492"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="573"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2076697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="714" mass="80985" checksum="E2A124D0C8CCF2F0" modified="2022-05-25" version="1">MGNSFSLELDESSSEQVFHPTIAPINNHIIPASAGVKNEKKEFEDVRKYFKELESRSNLTKFESDKLKRHKSAVLIQKIWKGYTQRRKWTLFLTKSQVLRELILTERDYVQFLRTILNVYFTPLKDLSTKVPEKGGTIMYSSIGSAAGNNINSLLQSGNGNNMTTSTSTLNLNLDQSPMTSDEVKIVFSQIEVLLLYNSMLLDKLSSRVLNSGWGENQQKIGDIFLEMIDFLKVPYSHYIMNFPACLKVLEKASKRPSYVLFIQECKSIPEVGKRDLQSFVSMPIQRIPRYVLLLRELIKFTSTCDPDHTSITKALTRMEAIADLINKQMKEDETSKEVIAISNKLKPPIPNLIEPHRRLIREGEVKIYKADGNNHDVSKGTIRPNSSVWKIHNPRFRYFILFNDCLLITKKTKSCYKVENNVSLEYSTVIDYNSSSIKIIEDTSRPVEKDDDDDDDTSSIGGGARSRSNTTENRDQQWTSSEVPSSKDRSSSFSSASSSSINIGSQKKPSPAKEGDRYYFKLHTLEYTAVICLPTLEEKNQWKNDFINVINQITEKSISFNPKWSRNNLIYSTSPSSPSSSNNNNNNNNSSGNNNKNFKDVFNSQTQYSFNSPQKGSLFTADSLLIKQSIQDLLSIPEQDKNNIQPPPETTATTTTDKKKTVIINNSNNNANTSPSQATEKLELNECVNDTVPTTQSRSTFDRIMNMIKNYNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7T9</accession>
    <name>A0A8J4V7T9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001532</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000037">
        <property type="protein sequence ID" value="KAF2077147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7T9"/>
    <dbReference type="OrthoDB" id="15710at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035327940" evidence="2">
        <location>
            <begin position="20"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="273" mass="30999" checksum="7C0A541A43CB04B8" modified="2022-05-25" version="1">MTGSWVFFALGALSAKSLAVVEGSWRYLGGASIVFHGIKTYVFGKLVRTIEYPDGEMVSIIIRRNNLEKLKSEIIRHQPALFPGVELSASDVKFLSRWTPPHFTEPCLKNKDLKLIADGGVVFWSRFDPIPKRGVTLDIKDKDHIKLDQAINFISKLGKYGTVEEALRLTENQENALIDLLLEKDEAILALHRNFSPYPHIFRYHALRYLQNKKGIPLDPFKPQQQQTTNSKQQQQQQPNSKTDKEKEKEKENQTDTKTATKAPSPTDLNAAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7X4</accession>
    <name>A0A8J4V7X4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">U3 snoRNP protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_001441</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in nucleolar processing of pre-18S ribosomal RNA. Has a role in the nuclear export of 40S pre-ribosomal subunit to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000034">
        <property type="protein sequence ID" value="KAF2077252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7X4"/>
    <dbReference type="OrthoDB" id="441771at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030692">
        <property type="term" value="C:Noc4p-Nop14p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007276">
        <property type="entry name" value="Nop14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23183">
        <property type="entry name" value="NOP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23183:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04147">
        <property type="entry name" value="Nop14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="121"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="317"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="914"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="150"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="162"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="317"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="328"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="388"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2077252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="933" mass="107209" checksum="D17B8C2C1D2EE0F5" modified="2022-05-25" version="1">MAKASKSDLVNQKKDKSVSKTSAFGKTTTAAGGSGVKVVNPFDRKVNKVKHEVLGKKIVGGEGNRGQSRYNAFEKRKKTLGIELKLQGKTNKFVDQRYGESDETMTEEERMLVRFQKEKMRNKNQYNLDDDAEELTHMGQSLGDKLIEDYQPDSDDQGSDDEMGKEFLDERTRFGGGDDDDENRKRSTKDIYQEIIEKSKAGKAERAREKLIKERLTNELDADFEAIRGELVLSSKVQATPAQEDDEFLKFQAEQQKANAVNSSASGANGVEKEKHDDFDELLVSLAGEAKARATDRLKTADEIFKEEKEKLEKLEQERLKRMKGDDVDFDDDQEIEPDSNRPDRLKKRKRENDNRPKILSADSLDECDEILKANGVEPAMYNPSLSSDEEGDDDEEGDEDEDDEEEEGDEDEDEDDDDEDEDEDLEDDEENDSDESEEEKEKVIKKKSTTTQEIDLEIPYTFEIPSSIQELNEWLENRNNEQKELILTRIRVCNHVSIKPQNKEKMIKYLPILYQRFIETAQGEQTQESVVTIDWKEIDILNRHIFEVSQDVPLASGSSAKEILDRAFKRITKKLEVSSKVNYWPTVDELLFFKLLGNVFPTSDFQHTVLTPATESLNLFLSSCPLRNSVDIVSSLFVSNILFFYLSSAKRYSPEITSLISSLLNTFVNHNPTLVSQPVNKKSKSTPTTTTSQWASIQSNFLPTILLPNDLLQIQSKDKKSPIKKMTPSEQLDFSILFKKPEDTYLISNQFKIDLLNYLLNFISKYIGFYSESEFKESLPTVFKHFVDILGRLNIDNDVIKTKVKDLVELINAKITEITSERVPLTLLTNRPAPLKQFNPRFNQVYTPYDKDPDQLRAEAKKMKGLVKKEMRGAMREVIKDNYFIQSEKFNKAQMERKENEAATKRVMSQLQNEQAEYKQYKKTKDRLDGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8A7</accession>
    <name>A0A8J4V8A7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000127">
        <property type="protein sequence ID" value="KAF2074834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8A7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35885:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35885">
        <property type="entry name" value="CARBOHYDRATE BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035261215" evidence="1">
        <location>
            <begin position="23"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="332" mass="36746" checksum="AC2912DFA6AB4816" modified="2022-05-25" version="1">MNKVSIALIFVLFCVTIYTSSGISVHEQSGLEGLDAAEYYGVGVWNNHSNINVGLVGLINSKEAIQMFVIPRHFVPYNYFQPTAYNYLTKTLTVLGADNMSDVLSIFTIDCQSWTLIKTVPVSQYSVLGGLSSLQSDDNSLMGFYTMIPAFYAGYVYKMLLSAEGTAVTVGGTQATYRGSVFDSDSQVFYFAFQNTSGLFIQSYDTSYTMQSEKEFTFVNTNKLASPTNGPLDMYYDSNSKSIWVGVYMASYLDYNCGGFAIMDWDNGNLVVQPNLVTPPLLIVVALTTIPSNTGVSVLTDLGGFYYIYTWNTVTNEPLSQRPYKTPLMAIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJS8</accession>
    <name>A0A8J4PJS8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010475</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001137">
        <property type="protein sequence ID" value="KAF2068198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJS8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="78" mass="8781" checksum="F6336B21AEF04D49" modified="2022-05-25" version="1">MINNDFVGFKFILTDYIKVVQVDIIDEAAMMTAQSSNLAIIDLAINNTLPSYESRPPLPFVKKLYQSSADQDNNIIGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PK87</accession>
    <name>A0A8J4PK87_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TRAF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_010347</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probable adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001054">
        <property type="protein sequence ID" value="KAF2068328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PK87"/>
    <dbReference type="OrthoDB" id="1630758at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001293">
        <property type="entry name" value="Znf_TRAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131">
        <property type="entry name" value="TNF RECEPTOR ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131:SF94">
        <property type="entry name" value="TNF RECEPTOR-ASSOCIATED FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02176">
        <property type="entry name" value="zf-TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50145">
        <property type="entry name" value="ZF_TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TRAF-type" evidence="9">
        <location>
            <begin position="47"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TRAF-type" evidence="8">
        <location>
            <begin position="47"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2068328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="254" mass="30021" checksum="62CBEE3F6F87CEB7" modified="2022-05-25" version="1">MESPKNIINLDYEDDYHVINRSPSTLFFKCKYCNDNYPRSREGERNHYNACREYPILCGDCKMVVTRKDLLIHLKTSCENAIIPCPYVKYGCQDQFKRGEIKDHIVKYNYHIQFLLETIDPLNKRIEHLGSFLLSEDEEKREKYYDGHKNNNTTTDKNLANSMVTLYSHLITLDWFNKDIRWYALNSDGVFGCKFNLSASHPDFKSINISSIAYNHQMEDLKRCVYNITLHDITHKEFACESKYATQILKCIFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKR4</accession>
    <name>A0A8J4PKR4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000907">
        <property type="protein sequence ID" value="KAF2068615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKR4"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="61" mass="6659" checksum="6D5DFD1FBD3BEE6F" modified="2022-05-25" version="1" fragment="single">MEAMDTTSAPTTERVVLCIGKTGNGKSTLCNVLSETMDFKESSNSVSETKEHKKQEYTIDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKZ4</accession>
    <name>A0A8J4PKZ4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010211</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000975">
        <property type="protein sequence ID" value="KAF2068464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKZ4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035262627" evidence="1">
        <location>
            <begin position="22"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="132" mass="14918" checksum="DAC7873FB28C119A" modified="2022-05-25" version="1" fragment="single">MNTKLLCIALLCAILSQGIIAEPRIKNEWLCTFLAYAQCTWYNGRCHKIMIPTQVTSSHSFLKADGAPVEQVKPIEKVKLDFPDFPADVPSDFPTPHIPSGWVRKEIDFPDFPSDFPTPNFPKPSFPPGWMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMC3</accession>
    <name>A0A8J4PMC3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_008988</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000613">
        <property type="protein sequence ID" value="KAF2069693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035319243" description="EGF-like domain-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1301"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="6">
        <location>
            <begin position="637"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="5">
        <location>
            <begin position="790"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="4">
        <location>
            <begin position="1073"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="975"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="977"/>
            <end position="992"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2069693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1340" mass="149486" checksum="00727BBA67A343A2" modified="2022-05-25" version="1">MRVNYLFIFLFVVLFYLTGGSRGIDVTQPILPYGVEPYSTSGTCEFNFYLLLDNDFSATFDGIRAMACSLSATATNPMHDSINCNNVTPIYVNSTHVYLIVYSTVEVDTYLDIYLSLSISKGMRVVELRDINKEKIQYTCQAPPTYNNWFVSGTETRLVNTSFQLSTNRFQNYITIDKVFTRPLPEGYFYSSEASIKLKFTYITPQVYLMDYQFDITSTDKIPTTLNIMATSGTFTTIENPLARLAYTSLDVDAIQIFNFDNLPYMGTVLYYRSQTKMFPSLNVDFTPYSLTDTAILYFKSKDQTQIKIQVNNKFDSYGPETKQASPTGPSLDVGYMATERFALTSIATRARISFYSDYLAEPFYWYYPYGVVLSGGNRGTNISFPIVLPDSPSAVFVHYGKPEPYTTPLVISTQTETNLPYLKGYTLSVLDETTSVLQLEMGDDSGVYYASVNTSTSVFYVKMSHLVLGSSKMGKYEIYVPFKTATAFSVGIMDFQLNLKVYNYHLMNSEFGTPLLDQNIRSRIQSIEFSPNVGVNVESNGVDLQLFIGLNHTDVERDIVFVRVYLNPNIPTQTIYGVYDEKTNIYQFSPIRLPQNLMSAFLNYSVFINEQEISSDILMGMFGKSAELYVENKGNFDMMFPHVISFTPPQTNVVANGETELGWSFDFSDSFGIDTMIVGIAGDFDIRGQNFTCNGKGDKSYTCKVTYNISQEMCRSQTYFINYIYTNDTLGNQGEYVRESATSLHPLFIYENSLSTYDIVLVCPTSADETAPTIEELVMTQIGFEPYPIIQAKFSARDETPGINDQYTPVCFLTAINNQIISAPAKQTTITGFVRYQCEFANVPYGFGPYAVLSIFGLADKHYNFAKVIPVFTAHGNYTNEIMITQPTNPYIKSASSLEISSKKLILYGHNLLDTVVHIISKHPDSETLVIPDISTGSSLVLLDITPAYEYIIQVNHNTNKIVSNTIVLKGPLPLPSSSDDSSGSSDSMSPSPSPTPTTTPVVKKCGSDCGAPQGYGKCVNGGCVCNSPHSGIDCKSVIDTTPVITPDPVKPFVNVTIPGTNSGQTPKFISFVYVVALRELDNTDTLINNYVFNSDKWILVEGGSSKNEQVTTVQYKYVIDNLFNTTIVSTVQVFEKETNVTFGNQQLFMNPSTIKFTFNITSYPFSKSTNSLQLVINAALQSTEKVACSYKEFVDDQGNSQYLKLQIEDRSLFGRFIKFGMIDGREQVVSNSLLDNIYGGKELSKSTSDQSYIGLNIPYYNKYALLDPDFSVLVEQNTARDQANSICTSESKKLTNAQLAGIIVGGAVFIFIVVGLVIYLYTRNSSSTIAMKLRKIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMG4</accession>
    <name>A0A8J4PMG4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Histidine ammonia-lyase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PAL/histidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000460">
        <property type="protein sequence ID" value="KAF2070675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMG4"/>
    <dbReference type="OrthoDB" id="10051290at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001106">
        <property type="entry name" value="Aromatic_Lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10362">
        <property type="entry name" value="HISTIDINE AMMONIA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00221">
        <property type="entry name" value="Lyase_aromatic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="54"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="201"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="64"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="201"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="578" mass="63171" checksum="CF9E828238391A21" modified="2022-05-25" version="1">MDHEIILSSNNLTIKQVSLFASKKIKFTISEKAIDKIKEKRNIIIERLGENQSWPSTKAEQANEKSNINNNNSSSNSNSSTSSKSGSNSGEKESTSTDVIMNQQGTLIPILHEQLLINNSSVIGNQYLSDKVTRAALLIQLNLFCSGNSGVALDVVIWLAKRLNEDNLPLAIDGCSLGVSDQVALAQMSLPLIGKTIPLPNGNQPPIESENINKVPSLFPPPSSSPSSDQGSSSGSTNNNNTTTTTTNIKERSQTSIQLSMKESLSLINSNSITLAQGAILLNDINTLMGGLDVSACFALEAFRGNLNSINSIVAKVHNQTGQVQCNNNLQNIMNFSKLWDHSESRFHQDPLSFRCISQIHGAAYSAYDWCHNIYEKEINLSVDNPLISSEDYNLYSNGNIDTSLLALSMDTLRSALCKCLKISGERLIKLPWKPFGNIPPSTNANHFNNHSLIVNLCKLAASSIGKCCQLASPTLNLYGGYQLDEGFEDTAPSTPLSIENTKLLLEEGWKIITIEIIISIFCIKQREISSSSLGFGLRSLYNNIIPLIPLNQSVDNPIVFDTRKIESLIKTHISDNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMG6</accession>
    <name>A0A8J4PMG6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_008864</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000590">
        <property type="protein sequence ID" value="KAF2069813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMG6"/>
    <dbReference type="OrthoDB" id="10064108at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039739">
        <property type="entry name" value="MAG2/RNF10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12983:SF9">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12983">
        <property type="entry name" value="RING FINGER 10 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="285"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="176"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="490"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="713"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="50"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="84"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="183"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="203"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="214"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="511"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2069813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="752" mass="88125" checksum="BBDFB78CE49AEC09" modified="2022-05-25" version="1">MSNNTNYSEGTNKAYTQQPPTPKMNDPTTTTATSNNSGHSNHNNKKHYNKNNTQKNNSNSNTSRDSNNRDNNSRKNNYRRNKQYNNQQQPQQQQPQQQPYQEEFDEELQLAFELSKLEFEKEKQNHTSSDFISNNDNNRHSTFEQEHVHIDKMKTKKKNTIDMTHLIQFKPRNPVNYQQQGYRNDRNERNDRNDRYNNHHYHNNNNNSHYSHNQQHHNYHKQKQKQPIKKKDGDVDRRSTFLQANYLFKINPIGNYTSALKSADHFVAWNKVEQVVYPTETEFLCPICFDPPFAPKITKCGHVTCYSCILRNLQHTDKCPLCLVPILKEDLKSVQFKSIRKYRVGDKITFQLLCYNQETNVPFLSTQTISNEISFPTNNEPNAPFSRFSIINNIDDIIEKELEQIYRAQAIADSEHEVETIVSLSEAEKDILERQKNFTKLMESKLHNDEKNYITVSSPQQQQKQHEFDSYQCSNNFEIRVALENYYNSVDSPSSSEDDSLDYNSSSGQLTPPNNINTTPTTTTTTTTTRTNSNSNNNTTQQQLEPTTIISNLTYENQPFESIYYLYQSNDGQDIYLHPLCMKILDREFKQGKNLPASFESNIVELETFEVTKKSRHLFKVLNMVTLTSSITFAEIDLTNLVSKSTLSEFASQLKQRKDIRLKKKRDEEKKLKIKEKERLTDIKRSRELSQIYQKEKEAEAEKEKQRLEEYRLEEEERLKNQPAQSSKKLGFLEAIQSHKNIDSIKEFPSLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMI1</accession>
    <name>A0A8J4PMI1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
            <ecNumber evidence="6">4.2.1.93</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ATP-dependent NAD(P)HX dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_008753</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(6S)-NADHX + ATP = ADP + phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19017"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(6S)-NADPHX + ATP = ADP + phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32231"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.93" evidence="6"/>
    <dbReference type="EMBL" id="AJWJ01000572">
        <property type="protein sequence ID" value="KAF2069924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMI1"/>
    <dbReference type="OrthoDB" id="8110916at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047453">
        <property type="term" value="F:ATP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110051">
        <property type="term" value="P:metabolite repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592:SF0">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 6" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YjeF C-terminal" evidence="7">
        <location>
            <begin position="4"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="157"/>
            <end position="163"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="197"/>
            <end position="201"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="216"/>
            <end position="225"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2069924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="308" mass="33626" checksum="EDCCF70DBC4270F4" modified="2022-05-25" version="1">MTHILDLFKPMIPNLLANLHKGQAGRVAVLGGSKEYTGAPYFAGISSLRIGGDICHIFAPTEYGTATALKTLSPELIVHPIEKSDPSDIIPWLLSLHVLIVGPGLGRSSSAWGCASEVIKAARNINLPMVLDGDALRLVCDNLDIIKGYDKVILTPNFGEYKSLSDSVKKMIGDESNSLLKPEQIALELGNITIVQKGKEDIITDGQQVLSVDEEGMPRRCGGQGDILAGAIGTMYAWSNLYYKYNFSEDAKECQYPIAMLSSYAACALVRRSSYLAYQKTKRSTITHDVINHLSQSFEQLFPEDVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMT4</accession>
    <name>A0A8J4PMT4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">palmitoyl-protein hydrolase</fullName>
            <ecNumber evidence="4">3.1.2.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Palmitoyl-protein hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_008882</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Hydrolyzes fatty acids from S-acylated cysteine residues in proteins with a strong preference for palmitoylated G-alpha proteins over other acyl substrates. Mediates the deacylation of G-alpha proteins such as GPA1 in vivo, but has weak or no activity toward palmitoylated Ras proteins. Has weak lysophospholipase activity in vitro; however such activity may not exist in vivo.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>S-hexadecanoyl-L-cysteinyl-[protein] + H2O = L-cysteinyl-[protein] + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="3.1.2.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AB hydrolase superfamily. AB hydrolase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.22" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000593">
        <property type="protein sequence ID" value="KAF2069800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMT4"/>
    <dbReference type="OrthoDB" id="2418081at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000276">
        <property type="entry name" value="Acyl-protein thioesterase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050565">
        <property type="entry name" value="LYPA1-2/EST-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003140">
        <property type="entry name" value="PLipase/COase/thioEstase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655:SF17">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02230">
        <property type="entry name" value="Abhydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0719">Serine esterase</keyword>
    <feature type="domain" description="Phospholipase/carboxylesterase/thioesterase" evidence="14">
        <location>
            <begin position="11"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2069800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="232" mass="26024" checksum="C9D3B590C69AFC6B" modified="2022-05-25" version="1">MIKNISAILKNSVIQKEIGKHTATIIFQHGLGDTGDGWADVMEMIQERGNDHIRFILPNAPIQPVTINGGLSMNSWYDIKSLTDRGDENKEEVDDSKSIIEAIIKNEIDNGIPSERIMIGGFSQGCALSLYTFYQTPYKLAGCMGLSGYLPLLPKFKQLMGPNSVNKDTPLIMFHGEDDQVVRLSWGKKSYDTLKENGINGEFITFPYMGHHASEEEINLMNEFVKKRLPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN47</accession>
    <name>A0A8J4PN47_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Anhydro-N-acetylmuramic acid kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008669</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000557">
        <property type="protein sequence ID" value="KAF2070012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN47"/>
    <dbReference type="OrthoDB" id="5427593at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006040">
        <property type="term" value="P:amino sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009254">
        <property type="term" value="P:peptidoglycan turnover"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005338">
        <property type="entry name" value="Anhydro_N_Ac-Mur_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30605">
        <property type="entry name" value="ANHYDRO-N-ACETYLMURAMIC ACID KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30605:SF0">
        <property type="entry name" value="ANHYDRO-N-ACETYLMURAMIC ACID KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03702">
        <property type="entry name" value="AnmK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="454" mass="50292" checksum="6B1D8CF6F85EF8D5" modified="2022-05-25" version="1">MKFNIVGLMSGTSVDAIDVSVFSVRVDKHKPIKDNNADQFTYKIKERAFQKVPWSESTRLLIFKLFDPEKSNVVDLCEANFILGKEFANAVNKVLIDNQINSNEIDLIGSHGQTIWHNIVRENNDEKVKVTSTLQIGCISVIANITGITTVGDFRVADVINGGQGAPFASIFDYLMCRNIIGDGGHDGDGIGIQNIGGIGNITILPNNTSKYEPLSFDNGPGNVLIDWISTKISNGLLTYDKDGEMGQKGRVNQQLLDMMIKETPYLGFSPPKSTGRELFSFQYAQQWYDIGKQQCGIQDDTDFVSTFTELTAVAIANSIREFTCIKVKHLYIGGGGSLNPYLMSRIQSNVGEHTLVHKHNQLGIDSESKEAIIFGLLGLLNTLNLGVDLSPFTGSKKPSSSNNNINYTPLVVLGKSSPGKNHLELIKKMYQFISFYWPVHQDKQYHQQLGRTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNE5</accession>
    <name>A0A8J4PNE5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008504</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000530">
        <property type="protein sequence ID" value="KAF2070177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNE5"/>
    <dbReference type="OrthoDB" id="186625at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061891">
        <property type="term" value="F:calcium ion sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042798">
        <property type="entry name" value="EFCAB9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47065">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47065:SF1">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="274"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="447"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="684"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="28"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="167"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="347"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="447"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="464"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="480"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="507"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="555"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="699"/>
            <end position="716"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="742" mass="82838" checksum="1846FF9E35CDB428" modified="2022-05-25" version="1">MKINIEFLKIIILGDNYSDEDDQDDDYYNNTTTSSSKNIKDQNINSNNNNNNNKSNSISLSESLSSSKLNKSPTLNNSNNTFINNQQQQQYTPNGVGGVQVFMSYQIVPQSLSPSPYSSVLSCSPSYDFSPPLPQTLNPTMSMDFEEILNPNIISPTSSTPRSYQYNSNNNNINNSPLNRTTSSSSSTSSNNNNKSTVNNNNNNNKYSSQNQQQQQQQQQSQREKRKPKTLFQKLYTPKGFDTTKNWYEKNTFNTDFILRESQFVVLLRYLTDLNNIQILDLFDTLDKDDLGFIGFEEFFLLISFFSSRESAQTTRFLYRHSRDMFRLISGSTASISLDNVNTTTNNNDSSFRSMNSPNSNSNNNSSTNNNSSKDNKDNNGNEDSVNGFINFDKFCKLGNMIGLSNDKILSEMERFNTVIFDRINYDTFLLFYFVILDRWDRNSSSFSSLSNNNSIGHSSSTTPHKRKSHKSHRTKSKSSPPPAPPPTPVPTPIQTTVNSPLISPTNLSNSMNSLNISSPTTTTTTTTTTTSSKSTKANIPKIAVSANQFHSITPPTPSPTTPTSPFKPPQSPPTNHQLLALQEWIDKLDLDSSHKKYVEDQFREHKITSLEYIHSSHIDWDHVLKKSTPRKAVVRSLYHPSIRTISAGNNYNRYSTTSIGSGSGSSNSINSGISSSSSINALVTPRAGKSPPPPSFDEVTSPTLNPRSHSTNSSVPTPPTRSQPPLKKTLSIISNIRKFVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNF9</accession>
    <name>A0A8J4PNF9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mediator of RNA polymerase II transcription subunit 25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_008084</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mediator complex subunit 25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000470">
        <property type="protein sequence ID" value="KAF2070593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="7690434at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021419">
        <property type="entry name" value="Mediator_Med25_VWA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12433">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12433:SF11">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11265">
        <property type="entry name" value="Med25_VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mediator of RNA polymerase II transcription subunit 25 von Willebrand factor type A" evidence="4">
        <location>
            <begin position="129"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="79"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="298"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="357"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="499"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="690"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="982"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1084"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1269"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1381"/>
            <end position="1456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1516"/>
            <end position="1594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="87"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="357"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="523"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="552"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="574"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="592"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="617"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="693"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="719"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="741"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="771"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1002"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1084"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1125"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1136"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1381"/>
            <end position="1424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1425"/>
            <end position="1451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF11265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2070593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1594" mass="175952" checksum="491744ABB256DE1A" modified="2022-05-25" version="1">MSTTTLYQTNGYQLQQQTNSGSSVGNNTMIHHGGVNNNVNNNNNNGVNSNNININGNGIPSTVTQHGISMVSPEGMSFKSSLADQFPSPNHSPQNNNSSNSPLSSLTSTSVSSTATTATNTTTTTPATKKTKQIIFILDTTAMMSHYFTYIKNTFLDPLAKSLQEESNSFNLDYSLVLFRDYPPYSDYIVNSSAITKDLKSLFVCMSSTKFTGGGFSIGNAVSEGICTALKVSSRKSADEKCFILITNSPPRLSPCRLCKRGNCFEHLTECINQKVKFSIICPQKNNSLIKDIYEKSRLNSQQQQQQQQQDDNDQQVFDGTDPQLFVKLIGLPQLPNTILASKNTLANNNISNGLVNNNNSNNNSNNTSPSTPIDNTTATPTTLVNNNPNNQAKPTIQNNNNNNNNNLPSPQIVNILQPSPNNINANTTNINGNNHSMVPPTTTNNTVNNNINNNINTTTNNNNNINNNNIPSNTIINHHINKFIGGHLPSPSINVNSPSITSPGTPGHVLSNNTPGFDVMDPSTPSTFNNNNNNNSNNNNNGNTFMPNSNLVNNFSTSGNSPSNPINLSQSTNLPNNGNNLNINPVQQNLDSALTSPNPTHQSHSPNHITSPSYIQPQQPNNINNNFVQNNSNNNNNKPQTPIMSIGSPSGSIQTPSSSSSIVPPPPSSTAKPVANQYVTPALPANRQIARSSSQSNFNSQPPTPPPNHTTQPIASPVSPSIHLNNSGSFANQNPPTPQTPNVINNNNNINNNNNNNNANISNQPATPIQPHTPQQQNTPVQPLTPQQKFPPQNFGANMLNYNKKGNTPGINEKIWSGFLGWRDGGTPFASIAAYCHNKIPLESPCANWPETLGITSIYNDNEMLQNHIKSAFQVVFNQQPSNLVDNGDFNFLLKKLGEEKNVAFVELPGKSMVLMIREHKLMGAILPEFVLAPKKDTQDNLVTIPTTNLNTTPIQNNTNNNNNNMVPSSQNTPIINNLGNLQNTNPSSPMPIHSPLQPQNTNNNNNSINNTVVNNNPTLNNNNNSNNNVNMNNRAQMPNQPQGIIQPNNFNQQPSQPQNNNAYKFPAQPNGVQQPITNNIGQQNNGNMAQQQNNNIGQQNNGNMVQQQQQQQNNMSHPIPNNGMIQNNMTQPTNNNNNNNNNNSNNNPQFVRQASNMNMNLNMNVAAINQLNNNLNNQQKLPNQQPIVVNNGVPMNSTNPNMINNNMAHKPAPQFINQPIANTPQAANMVNPIINTQNLTPQQQQQLKNLTPQQLATFQQQQQQKFLNFQKQQQQQQLNNNNNNPNVVNTNQNNNNPNNTNQNNNNNNSNSNNNSNNNNSNNNTQVQGQPQQNQSYQMQNQSMNQNQRPVVYNQANQPQPQPLTPTSNQNQFNIFQQQPLQQQQPNQPIQQPNQPLQQQQQLNQSPQNPMNQQQQQFNNSQQFMYPNNMMNQTGMMSQMGNLNPTQNPMGQIGMMNNVGAMNQINQMGNVLQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQRPMMFQGAPQQIQTQPMGVMSPQNPNPMMYSNVVTPTAIPSPTPQTVSSPSVEDEPSSKRLKRSSSSSKTTKVKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP85</accession>
    <name>A0A8J4PP85_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SD-repeat containing protein B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_007735</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000429">
        <property type="protein sequence ID" value="KAF2070944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP85"/>
    <dbReference type="OrthoDB" id="18774at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033764">
        <property type="entry name" value="Sdr_B"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17210">
        <property type="entry name" value="SdrD_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117074">
        <property type="entry name" value="Hypothetical protein PA1324"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035224818" description="SD-repeat containing protein B domain-containing protein" evidence="4">
        <location>
            <begin position="24"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="SD-repeat containing protein B" evidence="5">
        <location>
            <begin position="259"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2070944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="392" mass="43288" checksum="4304E860963E8116" modified="2022-05-25" version="1">MVKLLTLISLTVVLLSFYNSVHCRRLDSTNPVYYWGFRSPADFNKIFSQANNEGFIVWNGTNTKINIGQPIDQNITTQILGTNVPTNTSYFNHVDLLNFFGNSSYATMVAHFNCKDSDQTCQEFRFNESMPDFCLMFGSVDGYDQVSIDTHDKTLGYQTPSVSWTDIKNGSFSNLTQQCHPIEKSETSVGTTTLGCFDKTCDNPNFIILRPNDTLSYLKICFNSFSESEVIFYSFARCDLLGGIVTRRPLFAAFTVLGSVFLDIDGDGIFDPSKDVPIPGILVYLTTSSGGPVRDSTGVIPPVETDSDGDFALENIPRGAYRIRFDIDNTAYSATGKSNDITTEGTSKVNPNFSTDILILANSGRGIRDSTEEDNVNTEKVLPNINAGLVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP89</accession>
    <name>A0A8J4PP89_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007801</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000436">
        <property type="protein sequence ID" value="KAF2070883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP89"/>
    <dbReference type="OrthoDB" id="20956at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040291">
        <property type="entry name" value="DDB_G0287341-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35202:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35202">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="24593" checksum="01C7091D4D5F6B6C" modified="2022-05-25" version="1">MGVSNFYKNKFTTLLIVLIFASMALLIAGLATRWYKIVVYQQTVSLTQEFSFHATKIVRHIIDTDVYPHSGWSVYDLKKTHVFFNAGMAMGAIAFIVGIISLIFVTLKQLGVVTNNIVWHITRGLMVVTFISILCSVSVLSGITNAFKKDYESSQRVTDRFPYCYQACDNKWKGTETNELGDKITFGPGMGWILTVISGVPALFAIVVAFVFKHSDDPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPM0</accession>
    <name>A0A8J4PPM0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Carboxypeptidase</fullName>
            <ecNumber evidence="7">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_008201</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="7"/>
    <dbReference type="EMBL" id="AJWJ01000486">
        <property type="protein sequence ID" value="KAF2070476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPM0"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF3">
        <property type="entry name" value="RETINOID-INDUCIBLE SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035341627" description="Carboxypeptidase" evidence="7">
        <location>
            <begin position="21"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="516"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2070476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="554" mass="61647" checksum="6922B1B42C0A8FB6" modified="2022-05-25" version="1">MNKFIILCITLCVVGTLVESKLNFGSRSETDFKKPNPKPVFGSAEYYDSNYLKPLEAITETPNDYLVASLPGLKDIFPMYAGLITVNETSGGNIFFWFFPANVTTPTDAPLLVWINGGPGCSSLDGVFLENGPLRVNQDMSISINPYSWHQSANVLYIDQPVGTGLSYTLDDSGLATNDLELEQNFYQFIQYFLTIFSNYKSNPFFMSGESYAGHYIPHYANYIVTMNKNSNNNTFPINIQGLAIGNGWTHPIVQYESYGPFAYAAGIISIEQANAYTNLVTACQSQINAGNFMSDECDNVMGTLQSDSGSNTTSMVNVYDIRLYDPTGGVNWPEPGITYESDYLNRADVRQAIHASSVPHNWTECNNTVYQYLSNQDESSLFLFPSLLEQMRILVYNGQFDLICNHVGTTEYLDVLEWSGAEEWKNSSRYIWTSYAEDGSGDLVTGGYSRSVQNLTYLLVLGGSHMVPYDMPVSTQDMITRFLKNKDFKDIIQSQGAQIQPTPTPTPQTKNPLPIGAWIGITVGGCVFGILVGTVITYVFMRSKMRNPYTSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ10</accession>
    <name>A0A8J4PQ10_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">LMBR1-like conserved region-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_008686</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LIMR family. LMBRD1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000560">
        <property type="protein sequence ID" value="KAF2069994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ10"/>
    <dbReference type="OrthoDB" id="73273at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072665">
        <property type="term" value="P:protein localization to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050854">
        <property type="entry name" value="LMBD1_LysCbl_Transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006876">
        <property type="entry name" value="LMBR1-like_membr_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16130:SF2">
        <property type="entry name" value="LYSOSOMAL COBALAMIN TRANSPORT ESCORT PROTEIN LMBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16130">
        <property type="entry name" value="LYSOSOMAL COBALAMIN TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04791">
        <property type="entry name" value="LMBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="9" id="KW-0170">Cobalt</keyword>
    <keyword evidence="8" id="KW-0458">Lysosome</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="97"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="195"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="305"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="369"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="412"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="487"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2069994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="526" mass="59296" checksum="4A4F9ED462C3DB7C" modified="2022-05-25" version="1">MLNIGLGWAAFGIVILVLIVLNYIIVKYYSDKHESEAITTIIAVIGLTLTLLCVMIIPVDILNVSTMSNQQGNWTIDPTDISSRNFNVKMLYYDSNIYLSIYSLLLVLYGAVAVVSLILIPFAYFFYEEYDENETIGSRIYAGCKYTIFFIVFTIVLLVVGAFVRPGSSKPVDNQNVQDWIKQEVLNQNAIESSLLFTFACLSFLGFCVWATYTAYGLSAFPIGLIKGKRKISDDKSHINEDLYKTREKSKFYSSKFASGKSLSAKEESTLSLLKHKERALERRSSRLDSADKGIKKIFVIFRPFAFIFGFIFLLFSLLIVISIVLSIIDKISASVCGSACGFLTTYPKLKNPFDILLIKLAPYFPADYFILGFVIFYIFASTLSGIVRIGIRFLWVHMYDFQYRKTFPQGLLVACVLLMLSNLCLNMQIINLAPRYVMYGTQVFLNTTSLEVLPCSIEAPPDLCIMSQIGVLTSRIQLGTSFFGIIYYYATWAFVGTFLIGLVVSIIRKPQTNISSAYDDEDDYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQI3</accession>
    <name>A0A8J4PQI3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006756</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000326">
        <property type="protein sequence ID" value="KAF2071919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQI3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="29" mass="3303" checksum="1557B7160BCE95CA" modified="2022-05-25" version="1" fragment="single">MPSVSSKVLTRNNQVKPVTKVKLPQPRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRH0</accession>
    <name>A0A8J4PRH0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Non-structural maintenance of chromosomes element 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_007063</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the SMC5-SMC6 complex, that promotes sister chromatid alignment after DNA damage and facilitates double-stranded DNA breaks (DSBs) repair via homologous recombination between sister chromatids.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the SMC5-SMC6 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NSE4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000357">
        <property type="protein sequence ID" value="KAF2071615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRH0"/>
    <dbReference type="OrthoDB" id="361242at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027786">
        <property type="entry name" value="Nse4/EID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014854">
        <property type="entry name" value="Nse4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029225">
        <property type="entry name" value="Nse4_Nse3-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16140">
        <property type="entry name" value="NON-STRUCTURAL MAINTENANCE OF CHROMOSOMES ELEMENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16140:SF0">
        <property type="entry name" value="NON-STRUCTURAL MAINTENANCE OF CHROMOSOMES ELEMENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15412">
        <property type="entry name" value="Nse4-Nse3_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08743">
        <property type="entry name" value="Nse4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4 7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nse4/EID protein Nse3/MAGE-binding" evidence="10">
        <location>
            <begin position="64"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Non-structural maintenance of chromosome element 4 C-terminal" evidence="9">
        <location>
            <begin position="199"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="149"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="282"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="173"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="299"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF15412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2071615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="321" mass="37045" checksum="22423381C5C94DBC" modified="2022-05-25" version="1">MSQRLPEKTETERRKIRKDYRTLIQRVQGNKDDLISESDSLLSVIHASDKIYTAVDTPREAALDSELLNLTSQYSLEKAQKLKVSLNTFDTLGFINRVKVKLGEAEEEDGDNNTDAWTKLGRELSKLYKTNPQFDFMYGPLELEKKVRQRKKAEETKDDEPIGKTVQAEKLESTATQESETTSTRVQVMRSYINKKKKVDFLDTVVDKNSYAHTIENVFYLSFLLKDGHVALEPDENGTLTVPASRPPEEKDFADGRANIHHSVIKLDYNMWKQLSENIDQEFNMESEMDEMRKTRAASQPQPSQQEQQTSTQQDTKKRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRI2</accession>
    <name>A0A8J4PRI2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010428</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001105">
        <property type="protein sequence ID" value="KAF2068246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRI2"/>
    <dbReference type="OrthoDB" id="23586at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="347" mass="39814" checksum="047EF10504950FD2" modified="2022-05-25" version="1" fragment="single">MAEVEEKITNLDDIFYDKYVAKREQAIASDWPSILQQMIASNKKLAEEEAKSELQRQIDSMSIAEFESFSVFSDNKEKDQARVKADQYFLEHVQYHIDPKILEILEWKKPELNAERLKILSSANIKTWYTNYAKLWVVLVADDELKGKIKSKVIKEDLQKMTSSKIYAKQMMLTYNYAYCEIVPRMKLYIRSAVASKNWAKKAKDYYVSEEYRKIWMAKLREQQKNEDAINKLKDILKRNSQAAKLIDLIPKDINSLDYVTSNIRAKIDMLDPSGKTSDSVVGYLQNVSLANQILGSGISDEEFNNHLEANVTTIFKGYQELQAPTGAQKPITEFVKGFVGLAGGIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRJ9</accession>
    <name>A0A8J4PRJ9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000351">
        <property type="protein sequence ID" value="KAF2071675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRJ9"/>
    <dbReference type="OrthoDB" id="1062969at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="426" mass="47224" checksum="480488B2B0F02C7B" modified="2022-05-25" version="1">MQSISQDLNNPDHLSNFFPGAKLIGNAVDDIPSFKELHANFDANGVAGEILPLGMPMIQTVSGVREAYQNLDYDLIKQMENVASQDLNYVFQNMHSQGMNAFRRIELNGKPYYATWTNSGMVWSCTESVTPLLDPNLKYSAVVQFGTYSANTKIAGISRYNLKLPDMLVESVIASAFVKLFSGFVVEGLGFVASAFAARLGSILATWGIELTITMPEIVLPIIAACIVFTIVFVGLSYLWNWMNKRYTFRTQVFNWDQNNIWGASGQYCDNAVMPGQDGNSLGFSLPKMLPPGTVITPPGFGPNTTLDTVVSYAVLIYCNDSTFLRGNAMSVKFKLENGDTVGEDGFMWVFNNPRFNDNQMVILSGVQDPQAAFTQVYENNLWDPNPIRVKKPVSQNNNNLTYGVDYLSGAPDDLYNIIININPPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRR4</accession>
    <name>A0A8J4PRR4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_007524</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SHQ1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000407">
        <property type="protein sequence ID" value="KAF2071149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRR4"/>
    <dbReference type="OrthoDB" id="73639at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000493">
        <property type="term" value="P:box H/ACA snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039742">
        <property type="entry name" value="Shq1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048696">
        <property type="entry name" value="SHQ1-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007009">
        <property type="entry name" value="Shq1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12967">
        <property type="entry name" value="PROTEIN SHQ1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12967:SF0">
        <property type="entry name" value="PROTEIN SHQ1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04925">
        <property type="entry name" value="SHQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21413">
        <property type="entry name" value="SHQ1-like_CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="3">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2071149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="504" mass="58780" checksum="78AF778818D1E7C6" modified="2022-05-25" version="1">MIVPRFTVDQNEEFIIVVARTPYIRASEADFYLLGNQFKFYCKPYFLRLTFSHNIIENGKEKASYNVNTLEFTFYLPKENVGEQFNDLDLITKLLEKKSTNNNTPKIQLIDENDGTNLSETIDQQLDDEDNQDEDGDDENEQEEWEFDQHIEPELSLDELKNKVFYGFNDSYCNFFNGLQEEIQDIIDIPMIDSISKSERTLVRKEFENMNFDADRYMEIPVPITSTQFNPSNLEDYDDDEEVLNSETQIDKNETDLPDDIISQINSTLAKKVQIVNESDESTKPSTITTNTKPKLIVFTDDERDIMKNLPNKSYMISNENSLLLGLVDIIFSYAFNYRINLGDSNTESAWNICKVSTTLSCLETFTSLSSVIESSFKRAITYPLYRSWSLCEKVLEDTKTIFQLGKRCLLKCLLDIKKILEGHDFKHYLNRLYIDDYCVWLQHASSKKLKSLKSSLSAFKMDKSSIEWPLEAYEKLVEEEGMVWGEDYEEEGEEEEEQERSMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS46</accession>
    <name>A0A8J4PS46_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ABC transporter G family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_010171</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000956">
        <property type="protein sequence ID" value="KAF2068501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS46"/>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Pleiotropic ABC efflux transporter N-terminal" evidence="3">
        <location>
            <begin position="68"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="2">
        <location>
            <begin position="149"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2068501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="312" mass="34999" checksum="DB0ABCB920F6A5BC" modified="2022-05-25" version="1" fragment="single">MEFELKEFKRRSRNGLQDDLAIPETNDQVLDVTRGVGEYDAISNELELESHQFIHPANGNSERDLEANVPMETEEDFKLRKYFENSQRMAMENGGKPKKMGVSIRNLTVKGKGADVSVIPDMSAPFYWFASLFNPSSWKKSNGTTFDILHDVNAYAQDGKMLLVLGRPGAGCSTFLRVISNQRDSYVSVNGEVTYGGISAKEWKRYRGEAIYTPEEDIHYPTLTLRDTLDFALKCKTPGNRLPDEKKATFRTKIFDLLVNMFGIAKQADTVVGNEFMRGLSGGERKRLTIAEAMVSSASITCWDCSTRGLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSV9</accession>
    <name>A0A8J4PSV9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Golgi apparatus membrane protein TVP23 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_006298</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TVP23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000285">
        <property type="protein sequence ID" value="KAF2072380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSV9"/>
    <dbReference type="OrthoDB" id="2151161at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008564">
        <property type="entry name" value="TVP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019:SF25">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05832">
        <property type="entry name" value="DUF846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2072380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="206" mass="22955" checksum="079BCD158303B783" modified="2022-05-25" version="1">MKYSPFADEPVADTSIPVSPAQGGINGDQQQQPIEGGGKRHPVALVFHFLFKSIAIGFYILGGIFNIGFVLSFIIITLCSAFDFWTVKNVTGRLLVGLRWWNEIKDDGSNQWFFESVQNKSQINQRESFLFWVATLVTPGLWALFCLFSVFRLKLVWLIVNVVCIALSGANLVGYIKCAKDARKKVKGMAQSYIVGTIVNQAINKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT90</accession>
    <name>A0A8J4PT90_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006083</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000265">
        <property type="protein sequence ID" value="KAF2072605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="19599at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031248">
        <property type="entry name" value="RNF213"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22605">
        <property type="entry name" value="RZ-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22605:SF1">
        <property type="entry name" value="RZ-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="1933"/>
            <end position="2187"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="2375"/>
            <end position="2600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="4508"/>
            <end position="4533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="5139"/>
            <end position="5158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="5172"/>
            <end position="5200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="117"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="162"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="4511"/>
            <end position="4529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="5175"/>
            <end position="5195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="5298" mass="612696" checksum="393B81C69DE23E4D" modified="2022-05-25" version="1">[encoded data, 5298 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTA9</accession>
    <name>A0A8J4PTA9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009657</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
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            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000768">
        <property type="protein sequence ID" value="KAF2069026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="68" mass="7725" checksum="A53459BBEB6089AA" modified="2022-05-25" version="1">MSIIFNLSNFVSTRVNKANINYQTSVNFSNTGSNKFASHYIVSLGGPSYHINDNQNHVTYFGYPYGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTW7</accession>
    <name>A0A8J4PTW7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005201</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000201">
        <property type="protein sequence ID" value="KAF2073492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="10250320at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Cyclin_PHO80-like"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF26">
        <property type="entry name" value="CYCLIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="326"/>
        </location>
    </feature>
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        </location>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="296"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF2073492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="391" mass="45523" checksum="5294585A4D57C417" modified="2022-05-25" version="1">MEPTAQSKSSSSSSSSQQEIEQQQQQQIVNNNTNTQQVQEPPKSRGYQRMFKRLLHNRNSQPKAMVQERIIASYKVYGWLIHYDPAYESTAFRKEVLVAIDEIFEIVLAILDMMKCSNNLLIPIIIYADKFVYRSGIKHNQLFNLLLTSTVVSLKFWSESTQVNNAIVAEIFDFSLKDMNLMERRFLTGVDYNLFLNSNQVTNYLLQLYDNQQTFFSYFKHPIENPPQIVRKPFDLVQYQQAMIQQQQIAQLQMMQKQQLEQIELKHIIEIQKAQSFSNNYNNNNNNNNITTSNHSPSSANSTPCSSTTPSPPSYHPYGNNSTNHYKLNKKHITNQRYQTQQQQIQQQKQQIQQQQQQQQKPTTDESIYHQQNGSNSYHVVTVGQMVSPMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTY0</accession>
    <name>A0A8J4PTY0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006186</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000274">
        <property type="protein sequence ID" value="KAF2072499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTY0"/>
    <dbReference type="OrthoDB" id="21191at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2072499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="301" mass="35672" checksum="410986729B4511D8" modified="2022-05-25" version="1">MNHSIENIEYQDIFTYEIISTSCDSWHQFKSFYKDLVIFTRNLCSLLGLSSINYYHSVTLLQKYLFIISQKVKSKQFDFKNGIHYYYLIAMSCTLVSIKVNYSPMLPLKVRDVLNVGYHLLSNENLLLNDDYYTIKENVIKVEQHLLRILEFDVGLPKKMNQLPHPYLLNFLYFLESNQVESQVSYNILNDSILNLSIYCRKQEDTRFGYEEIAIFSLLYAFEMCEITIPIEINDLYLLLGFENTKNTNTKYNDFKFHMDLFYQTYDPDKEVTFRPEISIEICKPISLVEDKDIKDSQSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUH2</accession>
    <name>A0A8J4PUH2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_004513</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000161">
        <property type="protein sequence ID" value="KAF2074168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035311948" description="EGF-like domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1321"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="6">
        <location>
            <begin position="420"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="5">
        <location>
            <begin position="778"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="4">
        <location>
            <begin position="1071"/>
            <end position="1293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="974"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="981"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1003"/>
            <end position="1019"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2074168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1361" mass="150155" checksum="F0BC155C3231322D" modified="2022-05-25" version="1">MRDYIIRLFIICIFIYIGGAQQQAVVTPLGALFRQTESYASSGSCPYSFVAVGPIDLSGGGGINIFCSILASPTAPTCIPTDVFYNTTHFSIRGAISFTSGVVYSDIQLKVVKSVGSVPHTLPLVNQDGSAFIFNCQDAPSISSPIFDSLLDPIGHMELNTVDMSQSNSFNLYIKLKSDFVRPIPLQQFTLESADYIFFPYYIDMGTVGFSFSFQIDYQNVDLGHIELGWTGNSYSFFTSSPLDFPIGPLTLNSFIEFKVGSGWGYYLDTQTNSLNDIEPSNIAMKPLYGNSTNLKYFVKGDSIYPRKIVNYQNVPFIKQFPAPEPSSVIVSSSTTDFQDRFIDINFDTNSVFFTTLGPLLSFSFPNYDTSYPYGLTSYNGYTRHYRFPVMYAPSTQAFQFNVEFSSRVSFTDNLVPTAPDTSPPTLDSLLVVLLNETHSILRLGASDSGSGICLIKVLNLVQFINKNNLVSGNSLTGIYEMIIPHKKSLTTEITVTDCSGVSQFYSNQQLNFMFGIELYQTPSFRLETFTHISFSRNQVDVSGDPIDLVLYANISQTTQQLKESTSISVSIFFENPNNIPIVQGVYNPSKALYEFPFMVPPRLRPGLVRYTFNLNMDNTIDHMKLIGKFGYQATLTIINSETIDSTFPIVTKADSVNGQSDPLVWDLEFYDISGVKKVMVGITSEYDMQGKIFTLDGKNQTTFQETLVYKLDPATCRPMKYWISYIYTEDIYGNIGESFRYSNRDFHPFFKYDGSNFDFISLTADYCTVNILDNNPPIIQSITITPTTNSSIQQEQAQVLFRVSDDTLVSLVHFPVCYFTGMGNEVVSAVATVVSNSGGIVNYACDFIFPFTFGPQALLSIYGLSDIYFNYIGFSPSDLKALLSSFSPVYSIPSATTLIAIESTSSLEKSPTVLYIYGRGFKNDSKVEIKTDTNLWILVPNITTGSVLVLYDIEPSLFYNVTVLNLSGEKSLPVTLKGQSPVSSSSTPSADDSSVDSSSSPTPTPVTPTPVTPTPTPTSPICKTDCGASLGYGECKGTACVCNSPHSGLDCLSTIANTTIEPNPHIPSVNLTLDGENGGTTNTKSKFSSLIQLISLKELENGGNDVVSKHVFNNDQWINVPSESNDPDVKTSKYKYIINNSLNTTIYSTVQIFSQAKSITFGKQQLQMNPSTVKFTFNITSYPFSKSTNTLQLVMGASVETNEESGCSYQEFIQDDNESQYLKIQIDQVSLFGRFIGYGIIDGREESITNSVVNQADEIATQQTSNSQQSYIGLNIRYFQQTALLDPDFSVLIETKSASDQSNSICTSDIKSKKLSAAQIAGIVVGGVVFLVIVAMVAIYVLGKKSYNPIVLKLRRITKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUK1</accession>
    <name>A0A8J4PUK1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004474</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000158">
        <property type="protein sequence ID" value="KAF2074228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUK1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="179" mass="21074" checksum="7783EABECE5162BA" modified="2022-05-25" version="1">MNSKDTNLGGTIIQTRGGNRDLNIENLMKADQFIRNFFFNYYLETILYPWSNANSGEIINYRTTYYDSYGHGYGYGRGDLKMTEDLTFPIENFVTFKSSFADDQLFCDFIFYKFKIDLTLLLKQTDNNQIDSILYAFEVQTPENPADEKIIKSFKKILKQIRLSNPKLMKTKEERTPFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PV13</accession>
    <name>A0A8J4PV13_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003267</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000100">
        <property type="protein sequence ID" value="KAF2075438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PV13"/>
    <dbReference type="OrthoDB" id="20965at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18316">
        <property type="entry name" value="BTB_POZ_KCTD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045068">
        <property type="entry name" value="BACURD1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING ADAPTER FOR CUL3-MEDIATED RHOA DEGRADATION PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145:SF8">
        <property type="entry name" value="RE57120P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="610" mass="68878" checksum="B28B3E897A5172B6" modified="2022-05-25" version="1">MIKLNIGGKKFCTTKETLLSSQSTFFVGLLGGKFGNYVDEKGYYFIDRDGELFAPILGFMRTGELIIPDSMPMERIIKEIDYYGIESLLSFVNQRTTDTPNMGETYFISRIKSTLTKNSDGTSCKIDRVLVKANILILYFSNNTIDIWIYKGITFHWIKLLSFSSPLESDLKGMICSISKHSINNQTYKIILSIYSNNTVVSYKIYLDYMHLRTNQFEEIIKKLEHNIDFIHFFLNTFYLACISKDGFITIFDVSNFESKALNISNNQKITQIGQDIDHSLFFGTDRGIVYGVKQSPQRGWGWIIEEIYKLNDPEFFLSSFGYSSSSGGNGGGGSSSPSMASKFENILQLNNFNDGQGDQEYITFITGCSVKGTTPKLLMAIGTHDGHIHFAHKSLSGNDKFQFAAKFCVGINDPITKIILTEGVLVTGQTEKGVVKTYQYLLKNKLSGPATQCVSARHSHHFTNCTKSIYYSDILLFKIYTDLHFASQDYQALLGNIKSTVEGKSDKVGGRKKLQYNQMSVMVDTNNTLFLFPNSQKSLKADDMSPIYNYQAPIAKINLIDLTPVTNIVCVTFGGCEPNLNDQSYQLLTIHESLEIYCWDLKEIDKRFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVJ2</accession>
    <name>A0A8J4PVJ2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005012</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000190">
        <property type="protein sequence ID" value="KAF2073662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVJ2"/>
    <dbReference type="OrthoDB" id="442921at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16116">
        <property type="entry name" value="Ubl_Smt3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000202">
        <property type="entry name" value="Small ubiquitin-related modifier I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022617">
        <property type="entry name" value="Rad60/SUMO-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10562">
        <property type="entry name" value="SMALL UBIQUITIN-RELATED MODIFIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11976">
        <property type="entry name" value="Rad60-SLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="24"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="104" mass="11729" checksum="F14D4F5F340AD507" modified="2022-05-25" version="1">MSDNNNNNNNNNNEEVKKEGGDQEQINIKVLDSNGGEVFFKIKKTTPLKKLMDAYSSKKGVNPSSLRFLYEGNRIDYENTPKQLNMENDDIIDVTIMQVGGSNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW38</accession>
    <name>A0A8J4PW38_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004524</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000161">
        <property type="protein sequence ID" value="KAF2074180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW38"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="478" mass="53739" checksum="FF028B109E3FF7DE" modified="2022-05-25" version="1">MSSNENISFNSTIDGHEVTPSYSSDFHVTADAVVSLFEGSIDFDQIVAERALRTVANHLAPYQDYIDALVHITRPISTFQEFKTKVFNASFDYVLPDKFSHLHHGLVVLTSSLVQIEKYLSIFFNLLRYDSNVIKGILKNYIDGNPQESAPAVAPAVAPAVAPAEIYTHEPGPTYILQHMVPTVKNNNTPYGRRVAKTTSKNGISLTIPKCREFKRLFPARFYRASNTNSPDLDLNNPFSGIISSQILCLFNTIYQPFPWEISTKRILKPNPALFVANHNRYTKLSFKVRIVSTDPNGLDSEAAFTVTFNYKHSGNGGYSFEIAVARIDANHNSCKFIFELYNNVEVIGPDGRAFIREELLDQIESPSVFFRNTTRDFGNPTNVGFQRDLTIRNKYHAHSNHFKEGRLTGTGKSLFALIKSLGDIRADVIIPQYQIIFQSTFWISFICDLEPGEYSITLAYTNGQIPSSPQIPYFAIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW39</accession>
    <name>A0A8J4PW39_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003900</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000129">
        <property type="protein sequence ID" value="KAF2074797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW39"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="558"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="126"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="579" mass="67912" checksum="2414A2A2B979F4EA" modified="2022-05-25" version="1">MISNVLQKLIIHYYFNELKEEIESIPSFYGVREEIIKDVYQDKKRLSLVSKYWFQQISSFINFEMIYQYNRDEAQYSPSIYIHLNDTINNPFNLLKFSSNLSLDLNLHIQSLVSKHKQEKIDDSNDGNSSNNNNNNKNSKEIMFYKDYYSNVRRLSLDLGSLWKSNEIVQDLFRAVIEVSQNSPKLEYFQVLVPMWAIDSYAREKEFNQALLELGITDLHINFYNPSYHPFDQQQIESLKYFIKFHSRTIRSLTVQENIEDFQLFSDILVLLKDSLREINYSSIVNFGNASMIEDDSVFVTTLQSLSLDNFYIKLLTEDQYSMLCVKNNIGTSECSPISIIDTTRSKRFKVNIKDIISTIKYSPCIKSLEIKETNYNGSIYTRCNSNALSREITEMILKNMFQHLLDFLYIRVPLDIDCLTEYVLANAKAKHVLLDYKFKGTPSFYQALSTNTTIETIQINNQQLDMTDKQLLVESVLANNTLRYIRLYSKLNDDMLSKLIYKYDILDQNSVLLKRKSLEQQQQFLLQKEKEKEKERETDLIYYNSSKLLNHISDKSSFVLISSVFASLVAIAVFYYKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW68</accession>
    <name>A0A8J4PW68_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_002526</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000070">
        <property type="protein sequence ID" value="KAF2076173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW68"/>
    <dbReference type="OrthoDB" id="1742084at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04369">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15398">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15398:SF4">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN 8 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bromo" evidence="4">
        <location>
            <begin position="209"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="136"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="304"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="331"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="343"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2076173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="421" mass="47611" checksum="F77591F46FF77989" modified="2022-05-25" version="1">MDEQDNYLRKANSSIGTLLLLHVISKYEIDKPNFDWSNVQEKFEKIFENFVSQELLTKNVADNIINDLFSNQEVTKNYYNTLQNVFAKNHINLEIFLRNHRKEELNKEILDLDLAIKRKISLLEQLKLEQQQQQQLKKSGEISNTGGKKKSTTTASSSNNNSNLNTTTTPPITPVSPALVDLEDAKKKEEANNKRVLIGAMSKVWKSLNSHRFAYIFRYPITHEDAPDYDQVIKKRMDLTTLKKNLDDGLYHNVTEFKNEIILIFNNAMQYNAEHSEIFNMANSMKKYAEKELEIVFTTEELLQNNTNSGGGSTRSGRTSLPSTPNTLNRGRGKQPTPKRKNSSSNLEDYGSPISNPNTPSLDSNLESSDQETAPISAPTPASTEKTPTRRGRAAATTTPSKTEPPVTTPANEKKNKRQKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWG1</accession>
    <name>A0A8J4PWG1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000117">
        <property type="protein sequence ID" value="KAF2075040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWG1"/>
    <dbReference type="OrthoDB" id="19240at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013887">
        <property type="entry name" value="UPF0592"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35397:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35397">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08578">
        <property type="entry name" value="DUF1765"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="429"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="748"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="150"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="189"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="240"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="751"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="809"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="836"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="848"/>
            <end position="871"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="965" mass="107464" checksum="4A04F5C5E7E6C89C" modified="2022-05-25" version="1">MNEFTTKMNYLISKFIDPNLSISGGSNSNNGSGNNSGASSPLTTAGGSGSNSPNLNISTEKPKLKYKHWCTTVDKKIHTINGLMPLLEASFSTSEEKKKEKVLLSYQKFNTVLQELVALLEVTTYKPNITHKSLEWNSHTNSTPTANGESDSPPSSFFLSTSPGSPVVSPYSVSPTSTPIIVPDEINVHSLNNNNSNSSNSNSNGHHHHHHNNNSTTNGLLATINNYKDMVNNHKDKESSTNNNNSNTAGSSLTSAKATKVILITCREALLQVWGSLQSFIEKYFQLDKDFSLIYQCMLLICKRKEFDSSVSIIDPITQKPWSLSPNDILAKYRYRLYKTFFFVSSTLSSRSFYSIPTIHFCSKYLSLSYFRIPKVARMILESINPPENEIQDIISHFPCPSHLKHLNIVGDRPDKSPFHTSLMQMSSASNISNSNSSSNNSNNIPLTSSLNMQNNNSNNESLTYEELFDQKSHGWVDNLARKETVYFIFFKEWFLNCKYILGENVDMKSVPGFFTIAYSLLHEVKTRDQAQPPLSKPTPCLDAQFTLISSCRLDNMIEVMIKILFLKTSVYDVASVSITLSLSEAWLKEHGILSDSFDINFFCEGIEGIIKSDHHQLLIRAFQMLYNSSESFQGAFRKKLFGDLLLTKYFYQLFLHWDQPVRNAYHHLLLYKMVRIKRANLYESGFSIGEFSKLTHVNTPITKSLSPAKFIEGSKDESHNYGSSSRLVSNSAYSSMIPKPIYSSAPPPSILSSSSSSSPSSPSSPSSSSNPGSPSTSQIKKVAPPVPPRPRVFKSNRNPMPMPEGGISNNNNSNSNNNNSLESEDSFVFVENPNDHPPLPIPPPKPSHLSSSSLSSTPSPISSPAEPSSSKVAIPSSLKTEKFSKEYSLDIELFLEIEIFVKKVQDQMRDPELKYHDPILSHYVASSISEFKTYIAHANQIDAVPPKIIPLMFANSAAIANYRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWH9</accession>
    <name>A0A8J4PWH9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_004665</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000169">
        <property type="protein sequence ID" value="KAF2074014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWH9"/>
    <dbReference type="OrthoDB" id="541276at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF30">
        <property type="entry name" value="PHOSPHORYLASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="8"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="410"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="81"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="313"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2074014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="831" mass="94681" checksum="7157D0C09743B84B" modified="2022-05-25" version="1">MDEDNFECAICLDEITEFAVPFSSNQILGQKQCKHNFCFTCLKNYVENLIGKGQELSCPVCRELFQGFINNRSSNDIAKVAKQHSSTAKFLKVENQNLNNQLNDIKNQMDSQKQLLTEKLADVENQKSVTVKEINLLKNKMGDLEKELSEKHQERLKEIEVNQSKLYGQQQEILALNQTKSELEESYQNLYQQLNQHNERSNSLLSKLENYKQRNTSLEEHISQLEQERRQIQSKLESIESQTEKRIQEQLLLTKQQDTKVQQLHSIIEPQRLEIQALKSKLNESKKALDSTAGDSQKLLDIESAMSQLHVELARSHTVIETLNSKKKSLEKELEEFKRIKFNSSPSSLDMSSTANSILAATTKLSNKSLNLFNSGINYLYGNNNNPYQMNNINNNNNNSNLFIKSYKNFKIYEKRGNNKNSQIFRATLNNFNMVLKLIPFFKPMSTISSLYLSLTTGNTITQEQDISVFREAMILYTLNHSNILKLESITKDENTGKIYSALSPFVPFDLEYLIAENCRSSNSNGGNLLNSSGGSKKNSVLTTTLTFLNIKQIIYQLINTMAYIHSQELVHRDIKPTSILLFEDFQIKLCSFGQSCSVFTSFQNNIQIPNYSQSNFAYLAPEVILAILSNSNNTADINWKAVDMWSIGSIFLELLLKKNVFLNSIQKPSSLNNSGNGTPTFSLPKTAATNNRKTTMKASFGDDVGDDELSIDDFEQLRVPGNTKRQDLTCILDFMKMNSIQKILKENLNTSMPEQQQIKDAFDLLLALLDPRPDRRITAQNASLHRFFSNESYFNAHDQPSLATSDVSSSLTDTKIKDFLQEKCFDIVSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWL7</accession>
    <name>A0A8J4PWL7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_004152</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000142">
        <property type="protein sequence ID" value="KAF2074525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWL7"/>
    <dbReference type="OrthoDB" id="16692at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016314">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005547">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14497">
        <property type="entry name" value="PTP_PTEN-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000286">
        <property type="entry name" value="PH-protein kinase domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051281">
        <property type="entry name" value="Dual-spec_lipid-protein_phosph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014020">
        <property type="entry name" value="Tensin_C2-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029023">
        <property type="entry name" value="Tensin_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305">
        <property type="entry name" value="PHOSPHATASE WITH HOMOLOGY TO TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305:SF94">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3,4,5-TRISPHOSPHATE 3-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10409">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22785">
        <property type="entry name" value="Tc-R-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01326">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51182">
        <property type="entry name" value="C2_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51181">
        <property type="entry name" value="PPASE_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatase tensin-type" evidence="7">
        <location>
            <begin position="18"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="103"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="C2 tensin-type" evidence="8">
        <location>
            <begin position="194"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="409"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="658"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="738"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="878"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="658"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="672"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="699"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="763"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="881"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="893"/>
            <end position="904"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2074525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="904" mass="101829" checksum="237E4426604C7252" modified="2022-05-25" version="1">MYNSIVKAVRKTISADKTRFENETYDLDLTYICERVIAMSFPADGVESAYRNSIYDVSQMLNENHKDHYLVFNLSERKYEYGLFNDKVYDWCGFPDHNAPPLGLLFKIVKTIHKWLEADPLNVVIVHCLAGKGRTGTVIACLLLYGGLFNNASDAMKYFAVKRSNNNYGITGPSQIRYTQYFSDIYLNGREVNPSPVFLKSITMNTIPKFFLGPLKQGVCPVLNIYSATQKGVRIFTSAPIEGDTKETRTYLSGNATLIFEVRKIVRGDVLVVFSHVTPFYRVEQICRANFHTGMFSMPTLVLHKSELDGADGDKRFSSDFFLKVDFQEVVSNVGTQESVMLEHQKEIKKEVKWILHDIRDSIHNNSSNGSTSANNSNRNGSIFFLPKNNSEKIKQAKDIVAANKQGSFGVHSGFLLKKGQNFKSWRRRWFVLKENVLSYYKSPKDTTPAGCILVTDIVNLEIDIESSQREGYDYCFQIQTSKATYLISAENERDLEDWTEILKSAVRMVQVCGRLFLEILQVRYEFNNPTISKTPFDIISYLTVTLGKKRDTTSQQSQLFTPQYQYAGQFEVYDKDPLDLVISFWIRSSVLAVKDTLVADLVIPANQLQQKLNTEWRPFTMMKSNFLSNLSIQFGMVYRSENDINLYESALGLLQSPNANPNNSSSNNHSGLPTASPSTSFDQRRGGTFNFNQLNLNSSSSPSTSPIITTHTTGSSLGSAAGSGNIYLAKARENSVSGGNNNNIIINNSQPSLRNSKEEKSTTTTTTTNTNTNSNSSLNNSKESIDELYHIKSNSSSSIKEDNETSDKSFLEDLENQLNHIDEIDISIDPLLGTKFINNLSESNTNNNNIKVNINSNSNNSDHNEYNNGFLNNFNHTDFKNSNNSTNSENDLMNEFEPLDNSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWS3</accession>
    <name>A0A8J4PWS3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004069</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000137">
        <property type="protein sequence ID" value="KAF2074630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWS3"/>
    <dbReference type="OrthoDB" id="24201at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052050">
        <property type="entry name" value="SecEffector_AnkRepeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586:SF7">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="656" mass="75422" checksum="D98D0CFCBC5BA2F2" modified="2022-05-25" version="1">MNKSDLFFKIVRDKPLFRYLTSFLTISVNGFSFMNCPFSKAIIWRIFDEKVDLFFNVFQQDPTKHYYMDPTNNDLITLCASSAIPFDKFVRLFQQFETLYRDNAIHPIIASIATQQIEKVQYLLSKYRHLECSHGRLVEGIRFALATGPINAKVLQLIFDYVNAQSSLNLFDRGKKVFFDSLVESMLQSPHISQDVALVLYNSGLSDIFHSHFGIREVCNHGYLEVARLMARDRRSAMCHSITRITTYSLEVIKLFLNIRYLDKNFVHRMSFDSLDLLREYLAYFGWNSTEWLNFNIVDLLDESSLDILDFHCVSGQSIDPAALGGNLFLVKYLHHSKNGVASVGATNSCFEIVQFLIENRSEGFTDVHLTNCYFGDIKTVRYLHEKSLDPSNSVPILFSFRSIDRCASHGYYDIIKFLLENRKEGFSRDAINNAVSGGYLNIVKLLLSYNSTFTLFENSIQTAVNKGAYDVLEYLQTTVYANQVTPLMLAQKPKKEIRKAPAPLPLLDDDQLYLAHSSHLAHLVARGCYEQDDFDPLKFVKKFITNKKPPGHQILFLIHTIIHHTNFSINYRLDFLFRVLTVSITMGRLYVVDYFSSLKCKLNIINNDTDQSSSSVTLSTFFDESPKLKKSIYSTDFSNLSSISKDYLNHYGFNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX41</accession>
    <name>A0A8J4PX41_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_005101</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000195">
        <property type="protein sequence ID" value="KAF2073584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX41"/>
    <dbReference type="OrthoDB" id="77989at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010945">
        <property type="term" value="F:coenzyme A diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03426">
        <property type="entry name" value="NUDIX_CoAse_Nudt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045121">
        <property type="entry name" value="CoAse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992">
        <property type="entry name" value="NUDIX HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992:SF44">
        <property type="entry name" value="NUDIX HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Nudix hydrolase" evidence="3">
        <location>
            <begin position="88"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2073584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="319" mass="36239" checksum="5CAB8D9D66F97765" modified="2022-05-25" version="1">MNYLPNLIKNSRIINTNTSAFKKRSVASVALILRPSKNISDIISPFQCVCKSTSNIHKDNCIHSYIDLSKLSVSNHLSSQSQTPSSLINNSNGNKSLVDGDVGLEVLFCKRNRSGEVCFPGGGLERNELPKDAAERETMEEINVDISNGDNFALLGQLEDKYTFDKMKVHSFVYLQLCKETPDIQLAKDELVDCGWVDINHFILNSSSFQITPISQISQSKRLKSNPFLKFICDRVNVPFIKLPISSKEYTYKLWGLTLDMTSDLLKRMTGSSPIPQPRAINNNLIYNFYRIYRQFPQYILFFSLLVFSISFFTLQSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX92</accession>
    <name>A0A8J4PX92_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytosolic Fe-S cluster assembly factor NUBP1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_003041</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="10">Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of NUBP1 and two labile, bridging clusters between subunits of the NUBP1-NUBP2 heterotetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterotetramer of 2 NUBP1 and 2 NUBP2 chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000090">
        <property type="protein sequence ID" value="KAF2075668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX92"/>
    <dbReference type="OrthoDB" id="1741334at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000427">
        <property type="entry name" value="Cytosolic Fe-S cluster assembly factor NUBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03038">
        <property type="entry name" value="NUBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000808">
        <property type="entry name" value="Mrp-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028601">
        <property type="entry name" value="NUBP1/Nbp35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264:SF35">
        <property type="entry name" value="CYTOSOLIC FE-S CLUSTER ASSEMBLY FACTOR NUBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264">
        <property type="entry name" value="NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01215">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0408">Iron</keyword>
    <keyword evidence="9 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="69"/>
            <end position="76"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>ligand shared with heterodimeric partner</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>ligand shared with heterodimeric partner</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2075668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="318" mass="34566" checksum="AA4464F7153F8744" modified="2022-05-25" version="1">MSTDAKQLVQPPPEHCPGTESETAGKSAACAGCPNQQICSTAPKGPDPDIVEIEQRMKTVKHKILVLSGKGGVGKSTFSSQLSFALAMNQDTQVGLLDIDICGPSIPKIMGLEGEQIHISGQGWDPVYVQDNLAVMSVGFLLEKEEDAVIWRGPKKNGLIKQFLKDVFWSDLDYLIVDTPPGTSDEHLSIVQYLKTSHIDGAVIVTSPQDVALIDVRKEINFCKKVGIPIIGVVENMSGFVCPKCTKESQIFLPTSGGAEQMSKDMNVPFLGKIPIDPLIARSCDEGKSYLITFPESEATKQYKLVFDKILNSVDTKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXG9</accession>
    <name>A0A8J4PXG9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001970</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000051">
        <property type="protein sequence ID" value="KAF2076713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXG9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="67" mass="7678" checksum="1D39BDE74FCCA00A" modified="2022-05-25" version="1">MLFDSLTKLNKLSYSSRNESVNNSYISSIKQGQSSFETAADQYYTTKKSIFFGQPWWIATQKVNNSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXL9</accession>
    <name>A0A8J4PXL9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_003505</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000110">
        <property type="protein sequence ID" value="KAF2075200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXL9"/>
    <dbReference type="OrthoDB" id="8693905at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031152">
        <property type="term" value="P:aggregation involved in sorocarp development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06612">
        <property type="entry name" value="STKc_MST1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000605">
        <property type="entry name" value="serine/threonine-protein kinase 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024205">
        <property type="entry name" value="Mst1_2_SARAH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011524">
        <property type="entry name" value="SARAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF2">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11629">
        <property type="entry name" value="Mst1_SARAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50951">
        <property type="entry name" value="SARAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="22"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="SARAH" evidence="16">
        <location>
            <begin position="411"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="299"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="309"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2075200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="462" mass="52397" checksum="E5662DCB3175B179" modified="2022-05-25" version="1">MDTTQLVVPKDTMSKKDPEKFFTIVEKLGEGSYGSVYKAINIESGIVVAIKKVSVDNDLEDMEKEIFFMKQCKSPYIVTYHASFRKENEVWIVMEYCGAGSVCDAMKITNKTLSEEQIAVVSRDVLQGLAYLHSVRKIHRDIKAGNILMNHKGESKLADFGVSGQLSDTMAKRQTVIGTPFWMAPEVIQEVGYDYKADIWSFGITCIEMAESKPPLFNVHPMRVIFMIPNPSRPPPKLSEPEKWSADFNDFLAKCLIRKPELRPSAEELLKHPFITKSKSHSLLVPLIDEQDMIITQKGREAALGIEPRDEEEEDSDSDSDSDESEGSVDMGTVRPIKPRSIANSGEEEEEYDSGTMVITNNQSSYDTVVFNNDDEDTGTMKLKSTINNNNNKKKFVPAYMNQFNKDDASSTKYQNHTLDELKDLLEKLDLEREKEIQSIKEKYSINRQALLSVIDEKRLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXM3</accession>
    <name>A0A8J4PXM3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NELF-A N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003039</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000090">
        <property type="protein sequence ID" value="KAF2075666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXM3"/>
    <dbReference type="OrthoDB" id="2135488at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056557">
        <property type="entry name" value="NELF-A_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23553">
        <property type="entry name" value="NELF-A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NELF-A N-terminal" evidence="2">
        <location>
            <begin position="10"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="500"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="577"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="347"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="378"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="409"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="426"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="445"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="461"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="507"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="577"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="693" mass="78336" checksum="0FB0E5F6B5A4A43E" modified="2022-05-25" version="1">MNESRDIEGWLTSKLEEPWSSEKVSSLLTKEILQFFLSKFTKIDTIVRIKVLFSFLFLKKKQFGELESNINMLLAIADEEEDEWLKVLGQLLMNIGQEKISTDIDLESFKSNIKIINDELEKRTTLNFYPLENMYLNRNLVPTPTESLIVHNHFKLKKPCSELPVFTKKSVNLTPSLSGSKSNLLNTPPSSSISDEHQQDESSNNNNVGEYDMYKNGDHLHPLTQQYNHLHQSSDSSPNLKSDKLMMKNSASGTPTLRKSNLFIPKSHHIDISGSSGGLSPSLSGSSSGLYNSHGHHHQRNDLNSSSSAEKRGIQKKSKMHFIDLDQVRESQTSLQQKKRRQSIEPSNSDQNLSSSISNITTIPETSPSNNGNEEDQQQQQQSSSSSLTTTTTTTTTTSSTTPNKRKKSSDDSTTVEKKEKKPKKDKAEKDKDKDKDKEKEKKVKQTRGPRSKKAAKVADSITPGSTTSPTGTFSGGDSPILSSPILNKSMAEDLSGLSTLLNAVDQQEQNQQPPQQHQQHQQSQQQQQQQQHQQYPPMDYQKPLEPQYLLHRTTSPTINSHQGPPQPMINLPYHLQHQQQPIQQQQQHYQQPIQQQPPPQQQQQQQPNSTITLESFFESANQLTPLSRCIIEQFIIGNTNNPTPHEGNVKQIPLSIDIRSNADGEPVEETVIFEANYETGVWKKIKRKRKIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PY38</accession>
    <name>A0A8J4PY38_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007133</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000363">
        <property type="protein sequence ID" value="KAF2071561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PY38"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="579" mass="66825" checksum="98B0F5019FA6C8D3" modified="2022-05-25" version="1">MQSNNKTPQISIKDSGVDLFYSIFRNSFLKRVLINKVLENSIIVITNDDIISNSNNSKYKYLSNISAIEKLENNISIRFLINDLQQFKQYLNSIFKYVINDLKFGDGFDVSVTSTVNQEPGQQLIQFDFNLLHQDLHRLSFSIMDGAKFGDDIDNDDSCIITLPPSLTELNMQESYRYRFYEGIYRSHSYDRFLSNLPQSIKKLTLPYKYVIGLDKLVLPNSLCYFNYQPRDINNLKKLVMPTRKNLPLSCLCFDNIFIKRTQDLEWIRNHSWFTTLNFAVEDNDNEGVIKANVIPPNIEHLNANKYLIEQGSLPQTLVSLSLNGDHPLSNGIFPSGLHVLHIHHLHQELIKHLLPESLEILTIQRCSKPIPPNTLPSNLLNLDLRIYEFNFTVGLFPSSLTQLSVTKYNNSLDPFVLPQCLKKLKIIQFNNQLVANSLPPSLTKLKIPEYDRSFDLLSPLPNLKSIEVYKLNESIVELISNNIQDLKISFNRMDYNVSLYHTSINRLFLKSNSSLKSNLYARLLPQSLVKLKIIHIIIEPNMNSILPNSCVYLETDFINLNLDLLPKSIKYLNKKFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYD6</accession>
    <name>A0A8J4PYD6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003905</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000129">
        <property type="protein sequence ID" value="KAF2074802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYD6"/>
    <dbReference type="OrthoDB" id="5425247at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="192" mass="21584" checksum="42C1572B6ED20B4D" modified="2022-05-25" version="1">MVNHMVAPATPEAITQPPQLITTKSVADAFRNNDWNTIYLLQAMADLDLQPIYKRFEEDPDKSWVLAHDEATGRMYYVSYVNTGSYAWTFSDNVDFDPEPGKNVVYWLGLAKFTSEATISACLSFFVNKLLRLDALFTIGSLVLPIFVPCAVFVIIFIGISYLLRWLNKKSLSLPIYVIGIQDMIGTLGSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYK8</accession>
    <name>A0A8J4PYK8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phosphatidylinositol 3-kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_003755</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000122">
        <property type="protein sequence ID" value="KAF2074922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYK8"/>
    <dbReference type="OrthoDB" id="67688at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016303">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF66">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="8">
        <location>
            <begin position="357"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="PIK helical" evidence="10">
        <location>
            <begin position="447"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="9">
        <location>
            <begin position="701"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="90"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="257"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="257"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="288"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2074922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1029" mass="119803" checksum="EAFA7AE0A6FBEF7D" modified="2022-05-25" version="1">MSTTPTLPTTTPIVSEIPSSIFIVSNGVTKKFPPNYVKFSKMVQLKVLEAPKGHPRLNIYLPLNQVELEFVLLFLESLHKIIRNAITNSTTNNIHNSSNNNSSNNINSNNNNKSKNNNTNVAHYSNEKYRWLNSYKLDKVTKGELYKSISLRGQGLLIPRLIMAADSFDIIDNYLVRAIRNNLESYLSNCQSQIDASSHFGFISQEFQFTDLELEKIEREKHFGMYPLRDIATEQSFLYNSYADRKSFEKAMAKLKVNNNNTNSSKSVNKNKNQKKQKKPKENKQKEDQDEEELKEEEEEEQEENEHVEEEEEEEEEEAEEKDDDFESTIYDIVDEVLPRRPEVPMHDSKRCQNSSCHVDFTTLNRKHHCRNCGKIYCSKCSSLYVENSELPKLMYYNHYDWFGKERRVCKSCFETIRLPTDCHDTIKALKLLALRFDMLRRFSCVCPLWRKSSILYLSEVRFIQYKFPTHSFSDFERKVLWRNRESFTGHSLWLLKLLISIDYNEISLEKCKKVLSCIEPDSKKNCACYLLMCSGGCRPTISPLNVIPLLDKNVKSLEIRAYAVDILKQLDSILFVLPQLVFLLRYEPLDHQLLLEFLISKALSSKELAYSFLWELKNNQKSIGYANRYEQIRFKLISALSCNDSEIIESFSNGFELVHILQQHPVSNTPNMEHLVKLKSLEKSKLIFPVNPVYRILRFDIDTAEIIDSATKPIKLKCLCEHSEQENHKVFTLMLKKDDLRNDQVIMNIVGIMDEMVSQKMGEGFPKAVTYRVQPTGEDFGFIEIVENSKTLATIYKDHPCGVMSSFSDPTGRDRHTFINKVDLFNTTLATWIVFTHLLGVGDRHHGNVMITEDGKLFHIDYGFILGKEPKPTPNFIRNDKNFEHLFFNSEANKKHFRDLCVELFLHLRRESNLFLYHLLFLTRFEPNLNNRFNEKYIEEEVASRFVPGLSDAEAAEHFLYIIEQNTFRPIIDFFNKNRNMLQNLTDKISSSCGYLQDYLTKLPSLWGPAQTPSDLQDPSYHNHGDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYS5</accession>
    <name>A0A8J4PYS5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000139">
        <property type="protein sequence ID" value="KAF2074604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYS5"/>
    <dbReference type="OrthoDB" id="14955at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="94" mass="10709" checksum="4B13E3558D748405" modified="2022-05-25" version="1">MNKWIYQTKAHEKTLAPVWCEEFETTVTCPAEIVVELWDKDTLKDDYIGQATVTLTGSTLWAHTLVMTDKKGQRSGEVKLTFQEGSSRGYMTSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ58</accession>
    <name>A0A8J4PZ58_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">4-hydroxyphenylpyruvate dioxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_001835</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="10">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 3/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the 4HPPD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000046">
        <property type="protein sequence ID" value="KAF2076868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ58"/>
    <dbReference type="OrthoDB" id="414569at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003868">
        <property type="term" value="F:4-hydroxyphenylpyruvate dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006572">
        <property type="term" value="P:L-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07250">
        <property type="entry name" value="HPPD_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08342">
        <property type="entry name" value="HPPD_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.180.10:FF:000001">
        <property type="entry name" value="4-hydroxyphenylpyruvate dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005956">
        <property type="entry name" value="4OHPhenylPyrv_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041735">
        <property type="entry name" value="4OHPhenylPyrv_dOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041736">
        <property type="entry name" value="4OHPhenylPyrv_dOase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01263">
        <property type="entry name" value="4HPPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11959">
        <property type="entry name" value="4-HYDROXYPHENYLPYRUVATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11959:SF1">
        <property type="entry name" value="4-HYDROXYPHENYLPYRUVATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14696">
        <property type="entry name" value="Glyoxalase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009283">
        <property type="entry name" value="HPP_dOase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0408">Iron</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0828">Tyrosine catabolism</keyword>
    <feature type="domain" description="VOC" evidence="11">
        <location>
            <begin position="10"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="VOC" evidence="11">
        <location>
            <begin position="172"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR009283-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2076868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="373" mass="42493" checksum="951B7EF9A8E346D4" modified="2022-05-25" version="1">MDKNIGEYFGFDHITFWVGNALQAASWYITRFGFENVAYSGLETGNRQYATHVVKQNDIIYAFCSPLTADNETFATFVKRHGDGVKDIAFKVTDVKSIYDQAIAAGAKSVTAPHEIKDEFGVVTMATIEAFDDTTHTFVDRSQYKGAFLPNYSYKVVEDPLSKITGPVGLQFVDHIVSNHADQQMTPIVQWYEKVMKFHRFWSVDDKTIHTEFSSLRSIVVTDVNERVKMPINEPANGKRKSQIQEYVDFYNGAGVQHVALNTNDIIDSVSKLKQRGVQFLSVPKSYYVALREKLAHAPITVKEDLDKLEDLHILIDYDEKGYLLQIFTKNAEDKPTVFYEIIQRNNHQGFGAGNFRALFEAIEREQEKRGNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZL2</accession>
    <name>A0A8J4PZL2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mediator of RNA polymerase II transcription subunit 21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002767</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the Mediator complex subunit 21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000080">
        <property type="protein sequence ID" value="KAF2075919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZL2"/>
    <dbReference type="OrthoDB" id="526653at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.10">
        <property type="entry name" value="Mediator complex, subunit Med21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037212">
        <property type="entry name" value="Med7/Med21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021384">
        <property type="entry name" value="Mediator_Med21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13381:SF0">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13381">
        <property type="entry name" value="RNA POLYMERASE II HOLOENZYME COMPONENT SRB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11221">
        <property type="entry name" value="Med21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140718">
        <property type="entry name" value="Mediator hinge subcomplex-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0010">Activator</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="131" mass="15071" checksum="18E63CE85E21C69B" modified="2022-05-25" version="1">MDRITQLQDKLDDMFYIFGTCIGVLQRDAPPVSFKEQNELSFSQEWGHQIGDMATQVIDTSKLIESYIDSLPGLKKTETEQYENLKSLNSESNDTLKELKLTKSEAIEMLKLVKESIRYISEESKLIEDQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZN7</accession>
    <name>A0A8J4PZN7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">rRNA-processing protein FYV7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000057">
        <property type="protein sequence ID" value="KAF2076555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZN7"/>
    <dbReference type="OrthoDB" id="21300at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013730">
        <property type="entry name" value="Fyv7/TAP26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15657">
        <property type="entry name" value="THYROID TRANSCRIPTION FACTOR 1-ASSOCIATED PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15657:SF1">
        <property type="entry name" value="THYROID TRANSCRIPTION FACTOR 1-ASSOCIATED PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08524">
        <property type="entry name" value="rRNA_processing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="233" mass="28004" checksum="A270220CC344B93E" modified="2022-05-25" version="1">MSNREKKKLFNARGKISKTRDNKETLSLERFVKSKNSVYIPQKKIELQQKENAIKSKYNKYRENKVTSQPNDFYDKYMSADNDPMARFAIGDDSKYNKLDTRKKNNKKNDKKQETEAEQQEEQQEEKEETQENFVDKKDNNKNNEKEKKEKKEKETKQQSVEQQFKLAKQQYLQEKKEKQELFKQIAEKKEATKLKLQEKRADSMKYLKKTKRGQPIMLDKINRLLSKIEESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZW2</accession>
    <name>A0A8J4PZW2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_002613</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000073">
        <property type="protein sequence ID" value="KAF2076099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZW2"/>
    <dbReference type="OrthoDB" id="19165at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013714">
        <property type="entry name" value="Golgi_TVP15"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08507">
        <property type="entry name" value="COPI_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2076099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="18343" checksum="5F16159D8EFA9186" modified="2022-05-25" version="1">MQASRGLFSMSNGMNIVHGALSILLFVASIIVFTSKVIHFNGFTILLLSFWMVFTLLHGGKFYDQYYFMYTYGGRGVFYFFVGLLVWGSNESNFFSGVVSLLFMLFGIAGIGLGLFSNYEAPRPIIGPIDFSPYGQKSVALASQADFMDDSPSSSHQNTNNPAFTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q028</accession>
    <name>A0A8J4Q028_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Peptidase M8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_003225</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000098">
        <property type="protein sequence ID" value="KAF2075489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q028"/>
    <dbReference type="OrthoDB" id="527990at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.132.10:FF:000001">
        <property type="entry name" value="leishmanolysin-like peptidase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.132.10">
        <property type="entry name" value="Leishmanolysin , domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.55.10">
        <property type="entry name" value="Leishmanolysin domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001577">
        <property type="entry name" value="Peptidase_M8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10942:SF11">
        <property type="entry name" value="GP63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10942">
        <property type="entry name" value="LEISHMANOLYSIN-LIKE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01457">
        <property type="entry name" value="Peptidase_M8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="650"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="326"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="412"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601577-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601577-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2075489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="668" mass="74183" checksum="E9965EE98CDC127E" modified="2022-05-25" version="1">MKIKSLVLVFFISTILFYNVGYGFKVKQHTSLHNLNTAEDARIQQIIDKGYNRLVEDLKNKNIYHGNEKVIKTPSQAGPDGHLSYHCQHDVILDRKIDNIQKAFDKVKDKMSKGQKSDFARKRDQQTHHILQDPSPIRINFNTELITGTNDEFACYTEGALVSVGAAGGKTMCNDDSSGACLHKCTKKDIQTAGLQNLISQHVLTTIQDVLNDKIFVRDPKVKTVFDQDVADEFGGECDYGIKIPESFYSDGVEDTDIYVWVTLRPTSNNNTIAYAFSCNFPITQDGNLGRPIGATINFNPIYFVDFIGSESGFYFNEYIRVGIHEMTHALGFSRTFYGSFVNYQTGDGYDGGTFRDFNYVSTTPSGDRFNYTRTGIVTPNVLSFARDHYDCSTQQMQELEDYGGSGTAGSHWEKRTAGEEYMVGFVSPVEPITNLTLSLLLDTGWYSIDPTSADPFVWGQGLGCSFLDSCTPDSWNYQGYFCTVDGATSCTGTRVGKGVCRLGRARGSVPKQYQHFTDPQLVGLDGAADYCPFYDVFDDDANTYYCTNAALQSEKANKDIGETFCENCRCFEYQNSETGIEQACWEQRCQGTSLQLYILGSWVDCPDSTTVVHQGVTVVCPKGYTQCGGKAASVPVGGENSSSILSSLAIHYTLLIVAIVTFIAYYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q049</accession>
    <name>A0A8J4Q049_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Myotubularin phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_001998</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000052">
        <property type="protein sequence ID" value="KAF2076685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q049"/>
    <dbReference type="OrthoDB" id="271628at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14507">
        <property type="entry name" value="PTP-MTM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF83">
        <property type="entry name" value="MYOTUBULARIN PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="86"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="119"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="156"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="190"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Myotubularin phosphatase" evidence="5">
        <location>
            <begin position="662"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="500"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1077"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1149"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="346"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="500"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1089"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1186"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="1">
        <location>
            <position position="884"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="819"/>
            <end position="820"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="884"/>
            <end position="890"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2076685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1198" mass="134521" checksum="6D3F0AF188CE9E0B" modified="2022-05-25" version="1">MSVTSLSYPGLNDSTLDSISKSNDKFLLKDFLKRYPNLIDARNAHQQTLLHLMAMNGNLYGIKFLLKKKFLGFGDKKIYIDPQDKDGYTPLHCSVNQGFFEVAHKLLSKGADPNYRTISGSTPLHLLAKFCSHPKSIKLATSLIENGAYINHKDSKFETPLHRATLHGDNIDFVKLLLVNGANPNITNKRGRTCQHMAIQEGRLDILEQFLLYGAEFHKTTSIGSPIEMGLSSPFPAIQTFFETRVSSTGENIMMVEKSVFLGKYVQNSFQATGDSFNANQTMQLFSGRLFITNYRVVFKLDSTNKLPTNNSHLSKQWPFSEDEEMCVPLTAIDDLSTETNHDSNDPNSSNHPEINISSTCNCNSNNGISSNSTGDLQQYVNSSSSNINNSINSLNLNQSNGNYASSDIYSSGSSFSSSFGGNNSGRHSISSLYNSASGASANNNNNNSMNQSGQLSCSICNNDITMSVPTHNMYYSSTLNSSVLGASLSSSTSSFVNSNNNNNNSNSNSSSTTTTTTIPTTSTTNSDESPNLVNNILNGGTQILSMIINNHQITTNYHLISLDSKKDFKVYKLYFLSNLLRDKVFDLINFHYKTKIQKNIKLNIANINIQKKIVEENIHMEDESRIEHIDRYESYGIFEESVPYLFAFVYKVSNVDIGTDGWLVYSPKREYARFNIYEDTSGLNSNLFWKVSTINRDYKYCSSYPNWFVTPRVILDRDLEKIFSFRSKGRVPVLSWRDNNGNSSISRCSQPLVGIERTRCAEDESLCENIAAITATKHLYVIDARPKLNAIANTANGAGFENISNYNNCKLVFMNIPNIHVIRKSLEKLVSAINPSSSVESSDSRWWSLIEESGWLTHIKSILQAAMFCADLVSKGNSILIHCSDGWDRTPQLTSLTQLLIDPFYRTIYGFEVLIEKEWLSFGHKFSHRLGHTHRSDECSPIFQQFLDCVYQLVNQFPVLFEFTPNLLLFISEHLHSCKYGTFLNNCEKDRVSDQVKSKTVSIWTDINRCLPYFINPFFHNKQDNTIIRPSLHTRCLEIWKSCYMRSETKNFWLHNSWMTQTHQSSLSALKILSSPPSSPSLNHHNMKTTPPSVSCNSKSSEIKLHRSRSFSGDSGNISKPNLKLCDIPTPNNNNSQQQNTITSFPFLKTTSGHPHPHATSAPLLGTHRPISKSASYEDNDCGGNNNINNNNNNINE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q053</accession>
    <name>A0A8J4Q053_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000096">
        <property type="protein sequence ID" value="KAF2075519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q053"/>
    <dbReference type="OrthoDB" id="10290201at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="538" mass="61434" checksum="86A2062387978005" modified="2022-05-25" version="1">MNTDSFYLIWRNRYLRDFIRNRVFENIEIEINSFEYLDDNHRFLAVFTEQHKLDYNIFIKLILCEQDDLIKYVNSPYKYLINDIYIANGMDVSLLAKTKRQKGNSLILRHDFSLLHHGLHKLLFFIDEYTINGGAATLPHSLTDLEICLSFSSYGPTNIQSRFVDFILLNLPPGLKSLTLPNNYNISPHIQCVLPESINNFVYVSTHENYKRFVVPPNKLFDKTLLLAKSSEDLQWLGNAPWIQFVSLDLNINETKDTIPPHVRRLKVFHNNIPIGPNITQLDSYSSHPQNYTLPSMGSNLKELYLYSHDDKLQKTNLPATLTYLHIYHYNRPLDSGVLPPNLKTLKLELFNQPLEIGNLPVGLTDIRLGSFNQPLKPFVLPPAVQTLFLDHFDQTALECNSLPTSLTRLKINLFKGSFTQRLDNLRVLELGTLNQSAATLLSNVKSISISPRSIAAGTNLKDTPIQKLSLGPCLNGITRLHPGFLPNNVKYLSLEGYSIDFVNVIPQGCVQLRTEDIHNLNPTFIPPSVKHLIDISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0B4</accession>
    <name>A0A8J4Q0B4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">LNR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_001176</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the stealth family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000026">
        <property type="protein sequence ID" value="KAF2077558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0B4"/>
    <dbReference type="OrthoDB" id="263283at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003976">
        <property type="term" value="F:UDP-N-acetylglucosamine-lysosomal-enzyme N-acetylglucosaminephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046835">
        <property type="term" value="P:carbohydrate phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016256">
        <property type="term" value="P:N-glycan processing to lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.470.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000800">
        <property type="entry name" value="Notch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047141">
        <property type="entry name" value="Stealth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031358">
        <property type="entry name" value="Stealth_CR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021520">
        <property type="entry name" value="Stealth_CR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031357">
        <property type="entry name" value="Stealth_CR3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031356">
        <property type="entry name" value="Stealth_CR4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24045">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24045:SF0">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-1-PHOSPHOTRANSFERASE SUBUNITS ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00066">
        <property type="entry name" value="Notch"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17101">
        <property type="entry name" value="Stealth_CR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11380">
        <property type="entry name" value="Stealth_CR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17102">
        <property type="entry name" value="Stealth_CR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17103">
        <property type="entry name" value="Stealth_CR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50258">
        <property type="entry name" value="LNR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1049"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="domain" description="LNR" evidence="8">
        <location>
            <begin position="251"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="296"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="555"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="649"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1076"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="310"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="656"/>
            <end position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2077558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1096" mass="125403" checksum="EEC472A65966BCB6" modified="2022-05-25" version="1">MERNSGFMKLLQFKFYSFLSSRWGMALCIVGAFVTFNTVFQLLTFMANQYDYNYTHEFNKQVAWNNNVGGVSYQNILCSQPIDIVYTWVNGSDPILLKAVSDLKRSKRDPTIPECVDKQVPEKDNCFKDDNTASRFVDNQELRYSLRSIESFAPWVRHVYIITNGQVPNWLDMSNPKVSIITHEDIFVNKSHLPTFSSPSIETHIHRIPGLSKKFIYLNDDVMFGREIFPDDFYTQHGGQKVFLSWPVPNCNDGCPNNWIGDGFCDQACNVTMCEFDAGDCNNSTGQMKTRWWNKNRNTNTNTANGGLTSFGTTTASNTNTNANNGGGVGFGTDKSKTFCSRGCPDSWVGDRYCDKMCRNVDCGFDAGDCGIEVMYSDMVGHDIKLNTSLIHLPNNTYSVYFNLTHVVGNGSITDGSHDNSNLVRTATISQKYKIMTLTFHKNQPYQTVAISISYDKMVMENNTSVSKSFDKSFNMTLSTQTETIPPAVVPSAVLTSLNKTKDSNTTANDEKEEQELALGVGIVSNENESDNDNNDGPMVVDEQEVDKNPPFIVTEQEDSTFYKSTPSPPINLKKVEGEEQQEGNVKEIGLLGFSTAGVRQPLMFEQDTDEQEEEQEQVLKKFKEIENNNNSNDDEADVIIQLNYKPSGSQETIADNNGNNNNNNNNNDDDDDKYQGSNQLAKHYQGMTFDQILQEKQSELLFNDILYTDDLKEVRNYRINRETERLLRDEGEVYPWETIQEQQHKSQNNRRKLLDMFGDSLKFVNRLYTKEFGSSPRKVPAHMPHMIDVDVMNEIQEKWSEQWDETSSHSLRNSKDMQYAFSFFYYIIHKKVPYNITQVWGQLDGNRDGYLSENELRTFAINLYGVPLKEPFWNQIKAHLYQVCMDHHYQTLFDAGDLDALSMIHQNHSLVEEAERIDCKITLEVIKSDNKTMEDMQKAYSKKPFYRTTIDGTDEVAFLMIENNATITQGKLDGIRQKRQKFICLNDNMNHTDPHSVKVVKVLHDFYESLFPRPSSFELPPGKLNPFTYIQDAKETVQEVKTRSHTSYYFIVCISMCVLLIIWKCRVSTSFHKSKKRSSSGARDNYKSKSNPFVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0N0</accession>
    <name>A0A8J4Q0N0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GST C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_006216</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000277">
        <property type="protein sequence ID" value="KAF2072461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0N0"/>
    <dbReference type="OrthoDB" id="14734at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03190">
        <property type="entry name" value="GST_C_Omega_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047047">
        <property type="entry name" value="GST_Omega-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016639">
        <property type="entry name" value="GST_Omega/GSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419:SF6">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE OMEGA-LIKE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419">
        <property type="entry name" value="GLUTATHIONYL-HYDROQUINONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015753">
        <property type="entry name" value="GST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01206">
        <property type="entry name" value="Xi.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01148">
        <property type="entry name" value="Xi_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST C-terminal" evidence="4">
        <location>
            <begin position="166"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="123"/>
            <end position="126"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="141"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="3">
        <location>
            <position position="247"/>
        </location>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="3">
        <location>
            <position position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2072461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="318" mass="36855" checksum="A7EA0160403E229E" modified="2022-05-25" version="1">MSTTTTRDTTILNLDNSIDKKGAFVRKDSSFRNLITKDDPVFKPELDRYHLFISLACPWAHRTLIARNLKGLQHVIGLTVVDWSLGGDGWKFSERDGCTLDPFFGFAGIKEYYFKADPQYSGRFTVPVLWDKKTNTIVNNESSIIIRIFNSAFNDFAKNPELNLYPDHLAKEIDEINDYTYEYINNGVYRTGFAKSQEAYEEGYYKLFEGLDKIEERLSKSRYLVGSTFTEADIRLFTTLIRFDAAYHGNFKCNRNQLKEFPNLSNYTRELYQMDIIKQTVDFKHIKSGYYSARHVNPNGIIPVGPNLSYLDIPHTRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0P7</accession>
    <name>A0A8J4Q0P7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DJ-1/PfpI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001647</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000041">
        <property type="protein sequence ID" value="KAF2077015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0P7"/>
    <dbReference type="OrthoDB" id="543156at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03135">
        <property type="entry name" value="GATase1_DJ-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006287">
        <property type="entry name" value="DJ-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050325">
        <property type="entry name" value="Prot/Nucl_acid_deglycase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01383">
        <property type="entry name" value="not_thiJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48094:SF12">
        <property type="entry name" value="PARKINSON DISEASE PROTEIN 7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48094">
        <property type="entry name" value="PROTEIN/NUCLEIC ACID DEGLYCASE DJ-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DJ-1/PfpI" evidence="1">
        <location>
            <begin position="3"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="199" mass="21497" checksum="BED947BCC18F5640" modified="2022-05-25" version="1">MVKLAILLSEGFEEIEAVTTIDILRRASIQIDSILVDNKQDRQVKGSHDITLVADKLLQDFSSAGNQLLDIYDGIIYPGGPAVKNLLKNDEIVALAKQFNEKGKMVAAICAAPQILAKAGLLKNKRITHYPGCNQYMEESGAFVELEQAAIIDGDIVTGSSAGNCVLFALSIIEYFKGIESKADMIKQLVISPSTGTII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1F1</accession>
    <name>A0A8J4Q1F1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">dihydropyrimidine dehydrogenase (NADP(+))</fullName>
            <ecNumber evidence="6">1.3.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Dihydrothymine dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Dihydrouracil dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">CYY_002353</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; beta-alanine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the dihydropyrimidine dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.2" evidence="6"/>
    <dbReference type="EMBL" id="AJWJ01000064">
        <property type="protein sequence ID" value="KAF2076347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1F1"/>
    <dbReference type="OrthoDB" id="4327079at2759"/>
    <dbReference type="UniPathway" id="UPA00131"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017113">
        <property type="term" value="F:dihydropyrimidine dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002058">
        <property type="term" value="F:uracil binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019483">
        <property type="term" value="P:beta-alanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006210">
        <property type="term" value="P:thymine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006212">
        <property type="term" value="P:uracil catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02940">
        <property type="entry name" value="DHPD_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1060.10:FF:000007">
        <property type="entry name" value="Dihydropyrimidine dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000027">
        <property type="entry name" value="Dihydropyrimidine dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.20:FF:000023">
        <property type="entry name" value="Dihydropyrimidine dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000056">
        <property type="entry name" value="Dihydropyrimidine dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000061">
        <property type="entry name" value="Dihydropyrimidine dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028261">
        <property type="entry name" value="DPD_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43073">
        <property type="entry name" value="DIHYDROPYRIMIDINE DEHYDROGENASE [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43073:SF2">
        <property type="entry name" value="DIHYDROPYRIMIDINE DEHYDROGENASE [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14691">
        <property type="entry name" value="Fer4_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14697">
        <property type="entry name" value="Fer4_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="13" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0288">FMN</keyword>
    <keyword evidence="16" id="KW-0408">Iron</keyword>
    <keyword evidence="17" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0521">NADP</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="20">
        <location>
            <begin position="929"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="20">
        <location>
            <begin position="962"/>
            <end position="992"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00030119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00032722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAF2076347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1011" mass="110254" checksum="02A6B8B328908D63" modified="2022-05-25" version="1">MTTESHDVSAVHDIEILGLNPTVNKHATFKPTAVSKKDKENWARLDNCKKGCELKNDFDDVKPTTLTERGALFESARCLKCADAPCQKGCPTQLDIKTFISSISNKNYYGAAKAIFSDNPLGLTCGMVCPVSTLCQLGCNLAASEEGAINIGGLQQFATETFKKMNIPQIRDPSLTPLDQLPDTYRVKIALVGCGPTSISCATFLARLGYTDVTIFEKEQFLGGLSSAEIPQYRLNYEVVEFEIKLMKDLGVKIEYGKRLGENGFSVESLKQQGYTAIYLGIGMPEPKIDDVFKGLTSAQGFYTSKEFLPQVSKASKAGMCGCKSKLPELNGKVIVLGAGDTAFDCATSAFRCGAARVTVCFRRGFSDMRAVPEEVDIAKDERCEFLPYVLPKQVIMREGKLVAVEFYKTEKGPDGNYAIDEDQFFRVKCDYIISAFGSQIGSVADSCKPLTFNKWGTADIDPMTMQAKTCEWLFCGGDLVGNGTTVEAVNDGKTASWNIHKYLQGLEGIPIPDTPQLPNFYTPIDLVDISVEMAGLRFPNPFGLASATPCTSAAMIRRSFEQGWGFAVTKTFSLDKDLVTNVSPRIVRGTTSGNHYGPGQGAFLNIELISEKTCHYWCKAITELKRDFPDRIVIASIMCGFNKQDWTDLAKMAQASGADAIELNLSCPHGMGEKGMGLACGQDTELVLHICKWLKEAVTIPFFAKLTPNVTEVKDIAKAAYDGGATGVTAINTVSGLMGLKGDSNAWPAIGQEKKTTYGGVSGNAVRPMALRAVSSIRRELPEFPIMATGGADSADATIQFLHCGASVVQICSSVQNQDFTVVEDYIIGLKTYLYMQSRQDLLAWDGQSPPAVHQENSKFKGLPKFGKYQKERIKIQVEENKTKDLLKVTNPLPPPAVAGKVPSVQDQINRAIPRIGRHDDLRRDQQVVALIDEDMCINCGKCYMTCNDSGYQAITFDKHTHFAHVTDLCTGCTLCASVCPIIDCITMVPRDSVYIPDRGLVPSATSAQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1T1</accession>
    <name>A0A8J4Q1T1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">POU-specific domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_005927</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TLS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000250">
        <property type="protein sequence ID" value="KAF2072761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1T1"/>
    <dbReference type="OrthoDB" id="5627at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000327">
        <property type="entry name" value="POU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010756">
        <property type="entry name" value="Tls1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13486:SF2">
        <property type="entry name" value="SPLICING FACTOR C9ORF78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13486">
        <property type="entry name" value="TELOMERE LENGTH AND SILENCING PROTEIN 1 TLS1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07052">
        <property type="entry name" value="Hep_59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51179">
        <property type="entry name" value="POU_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POU-specific" evidence="5">
        <location>
            <begin position="294"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="164"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="286"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="293"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2072761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="311" mass="35618" checksum="F2961083ED4DA17F" modified="2022-05-25" version="1">MSDEINKSVGEKDTNNKDDDDEDKSTTSTVIDKPVLKFNKKPSNKRYRKKTDDDESLTAESDSAIAIKTGDGKEETIDLQTLIEITKEKQKMRGKDKGISVGVLAESSPHVKQSFREIESKLDDSFIVHQEKQEVNVHLEKYLSEKMKKPTLAEMGKIGLQSISSISNDQKKDDDNDINNNKNQDNNDAVEGQVDGGHYSEKEKLKNSLYDIPDHLKPTNNTVSPDEQKTNWVTGIAEVPLPNKFKVKNIIETEKARQVLENNNLPKDFGKKNDHNFNQNYNRFLQNKKHKNPEKATDEETMENFKKRFRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q260</accession>
    <name>A0A8J4Q260_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000778</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000016">
        <property type="protein sequence ID" value="KAF2077900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q260"/>
    <dbReference type="OrthoDB" id="24318at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="99"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="110"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="511" mass="58505" checksum="B737E240B214C88A" modified="2022-05-25" version="1">MAKGDSTAVDTVFYSVFRNIYIKRRIRNCRFRDSVLRVTLDYLNSNNNHVYLSRFVNSDKVDNNIRIKVVIQNTKQFRQYLRSPHREIIDDLCIESYVSTTPPPPSLNGEIEEEEKEKEKEEEIEQEQEQESEEMFQHEDQELNPMSYDLDLFHHGLRRLELAYQRNVTHWSGALPSTLLELEITHPGYRQFKSEFIDHLVSHLPPNLTRLTLPPMYNISVECRAPESLIDLDYISQVNTLCHFVLPPNRVYQNLTLRASNRQHCEWLAAHPLITSVFMYGPDFEPNIIPNHILSITMGSQRSSDNLILHKKCGIFHNALQTLDINSSCESILGIGVLPPNLTFLHLFHYNQPLQAGVLPQGLKTLVLSSFDNTIEPQSVPSSLTRLELRSYCGSFESVGPLNCLKKLNIYEIEPSASILLANVYSIVLIFMEIDPLATLFDTSIQEIECESIIDNTLPIGFLPLSIRKLFLRNLFVTEQGSIPDGCIVVDTDLPIDKSLLPPSVTIFHNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2A2</accession>
    <name>A0A8J4Q2A2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000251</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000004">
        <property type="protein sequence ID" value="KAF2078501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2A2"/>
    <dbReference type="OrthoDB" id="10290201at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="508" mass="57891" checksum="BF4738456CB8365F" modified="2022-05-25" version="1">MNKLFYLVWRNQYIRRFIRNNQCRDLIINVDENYLNDNHQYLEVFTAQDKLDYNISIRFHKGNYVNNQYKHLVTDLNYDHTFITQSDFGLIDDGVNRLSLIVKDGQEGTGKLPSSLTYLELSSWNHRETGPVAEIVHHVLSNIPPNLKTLILPHGYLFTSNYVIPESITYLGCESRYDNFKRLVVSPNKVYRNCILDVYSVEALQWLHENTWINNIVIDGRVNITDTPIPPHVATIRVRDGSIQEYALPKMLEVLTCTFETPFSHLAHLKILCLLHYPSKLEKGLLPLSLEEFNVTYNSSLDIDVLPRLLRTLRLYRFNQPLGVGSLPNSLTHLYIGSFNQPLGASVLPQSLKVLKMHNFNQTWLPSLPPSLVELNIEKFTGSFEASSQPLDKLNTLNIGSLDASLPRILVNVKSINMSVHSIIDGSGTCLYHTSLKRLNLHLIRKTPLYPNSFPPTIEYLSLINADIQSMGVIPEGITLKTVFGTIDPKYIPPSVKSNKLPIKTIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3K3</accession>
    <name>A0A8J4Q3K3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001227</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000028">
        <property type="protein sequence ID" value="KAF2077454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3K3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008455">
        <property type="entry name" value="HssA/B-related"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05710">
        <property type="entry name" value="Coiled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="69" mass="7546" checksum="1E3D97C35EF71490" modified="2022-05-25" version="1">MIFNTISQIANPSQSIMKSSIKSGSLGGLSGSNQSTRRGCCGGRRNDIDIDIDIDIDIDIDSNRRKNWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3M9</accession>
    <name>A0A8J4Q3M9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF5898 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000449</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000008">
        <property type="protein sequence ID" value="KAF2078259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3M9"/>
    <dbReference type="OrthoDB" id="20660at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045417">
        <property type="entry name" value="DUF5898"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34871">
        <property type="entry name" value="DUF5898 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34871:SF1">
        <property type="entry name" value="DUF5898 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19250">
        <property type="entry name" value="DUF5898"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5898" evidence="1">
        <location>
            <begin position="368"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="510" mass="58392" checksum="FA28258FB073F201" modified="2022-05-25" version="1">MSKTTILTQPQIQKIFLDLNIDEDVCNQIASLLNSNYINTKEKLYIAFFDDMELRKKYNIESTTKKTIFTDLLPLVGEHYTLSASYVGNNQVATLTESQDFEFVDGLNLESESSLIWRMSNDGKLSELTNHSEAQIIFYIKLVFFEVFQALGLCGVLDLDHDYSLTYKNPTSTCMIGLQHQHGHPIGVVEVVKQGNDNILNSAHLDGKMLDSMLRLYNVYGQKNVFGIATTYQEWRIYWLPECGQYAKSKVVDAIFKESIVHDHFIPLYNTDLSIRSSVLPQTHQPRLICATPIISYTNRLLPQYLCSVVSKMYHRSFSNTPLYSPEKLFIQLTEKSWCWTLAPETILDLVYDIGAFGKIDSILLLQDFRGATHGRVWLACSSRGNVFVVKFPTDDSFMQMELTIWEKVWGLPTLKGKWNGAKGFLMPFVLPASQTDWEDPTFIPLVAQAVRSLVDALYHHNDLHRRHVGLYKKGGQKVVVFFDLSSMLTISPKEKEFYYLDMLNNLDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3X9</accession>
    <name>A0A8J4Q3X9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Isovaleryl-CoA dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="5">1.3.8.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">CYY_000276</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>3-methylbutanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = 3-methylbut-2-enoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:12276"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57344"/>
            <dbReference type="ChEBI" id="CHEBI:57345"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.3.8.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15 16">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation; L-leucine degradation; (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.4" evidence="5"/>
    <dbReference type="EMBL" id="AJWJ01000005">
        <property type="protein sequence ID" value="KAF2078409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3X9"/>
    <dbReference type="OrthoDB" id="9988775at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008470">
        <property type="term" value="F:3-methylbutanoyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006552">
        <property type="term" value="P:L-leucine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01156">
        <property type="entry name" value="IVD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000007">
        <property type="entry name" value="Isovaleryl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000003">
        <property type="entry name" value="isovaleryl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000004">
        <property type="entry name" value="Isovaleryl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034183">
        <property type="entry name" value="IVD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016578">
        <property type="entry name" value="HsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15 16" id="KW-0274">FAD</keyword>
    <keyword evidence="7 16" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="19">
        <location>
            <begin position="44"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="18">
        <location>
            <begin position="160"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="17">
        <location>
            <begin position="267"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="280"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="194"/>
            <end position="196"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="216"/>
            <end position="217"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="278"/>
            <end position="281"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="374"/>
            <end position="378"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="401"/>
            <end position="402"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="403"/>
            <end position="405"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR634183-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR634183-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR634183-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAF2078409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="420" mass="45858" checksum="008B1E8DE583135D" modified="2022-05-25" version="1">MFKTLLTFTTKGPIKQSLLTGKQFIGSKYFVRNLYTTPDIVGLSEDLLELQDSVRKFAQNELAPIAEQVDRKNEFPMHMWKKMGDLGLLGITAPAKYGGLEMGYTAHCIAMEELSRASASVALSYGAHSNLCVNQITRNGNEKQKEKYLPKLISGDFVGALAMSEPNAGSDVVSMKTNAKKVDGGWVLNGNKMWITNGPDADVLVVYAKNDVNAGSKGITAFLIEKGMKGFSTGQKLDKLGMRGSNTCELIFEDCFVPDENVLGQVGKGVQVLMSGLDYERLVLSAGPLGIMAACMDSVVPYLHQREQFGKKIGEFQLMQGKVADMYTLLNASRSYVYSVAKSVDNGFTSRKDCAAVILYSAENATQMALQAIQTLGGNGYINDFPTGRLLRDAKLYEIGAGTSEIRRMLIGRELFSETD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q496</accession>
    <name>A0A8J4Q496_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NmrA-like family domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_000130</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NmrA-type oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000003">
        <property type="protein sequence ID" value="KAF2078505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q496"/>
    <dbReference type="OrthoDB" id="3358371at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd05251">
        <property type="entry name" value="NmrA_like_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000181">
        <property type="entry name" value="NmrA-like family domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051164">
        <property type="entry name" value="NmrA-like_oxidored"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42748">
        <property type="entry name" value="NITROGEN METABOLITE REPRESSION PROTEIN NMRA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42748:SF4">
        <property type="entry name" value="NMRA-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="8">
        <location>
            <begin position="2"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2078505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="301" mass="32615" checksum="C652305309619912" modified="2022-05-25" version="1">MSKIVSVFGATGSQGGAVVEALLKDGKFKVRALTRNVDSPKAQALKAKGAEVVKADLDDSVDQLAKALEGSYGVFLVTDFWTVFQKELEQGKKAADAALKAHVKHLVFSTLSHCIKLSHGKLSVPHFDLKAEIEQHIRDLSAKNPEFVSSFVLAPFYAQNLSTYFVPRKGEDGSYSLSLPLDPSQGPMEIADINDIGPIVSGIFNDPKKFSGVAVPFQGSVVTGEQIAEIISKTSGKKVSFNFVPPKVFATFFPGAEEMATMFQFYSEFGSFPGKDVKIAQSLATQSTLEQFLTKNPIKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q516</accession>
    <name>A0A8J4Q516_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_000197</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000004">
        <property type="protein sequence ID" value="KAF2078447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q516"/>
    <dbReference type="OrthoDB" id="10262475at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006886">
        <property type="entry name" value="RNA_pol_III_Rpc5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12069:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12069">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES III 80 KDA POLYPEPTIDE RNA POLYMERASE III SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04801">
        <property type="entry name" value="RPC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="106"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="254"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="394"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="534"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="563"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2078447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="965" mass="110518" checksum="07F38AECAC20E4F8" modified="2022-05-25" version="1">MNSIDLSFGNILLAKEMSSEPLELMLPPTDAISSVNFCPSSNNLLVTSWDKSVRCYDVNTNTIKWQYNHIVPVLDGCFPEKTKAFSSDIHGLIKYYDPVAGVERDLGSHEGGAKSIVYNTSTQQLFSGGWDSKLKVWDTRSKEPEISCSDLESKIFTIDCAVASHTLVIGTADKHVTIYDIRVMDTPVQKRESSIKYQTRCIRTYIDGKGYALASVEGRIAMEYFDTAPEVQAKKYAFKCHRATENNVETVYPVNSIAFNPVYGTFATGGCDGNVYFWDGQNRKKLYFLKHYPTSVSSMSFNSDGNILAVASSYTFEEGEKDHPPDQIYIHNINEIYLNRIATMTTKQVDVEINDNMDVDSQKSNKTTTTTTTTTKTTLDDDFVNNILLDDPLEGLENKKSNGNGVGSGGGGGDDDDDVLLQEIPVFLSQTLSNNLYLFQYPLRQPWRPYDMSKLEEFRIKPKQQKIEMDLSLDEDTFNPDSPLANNKFTLSSSTVLHRTNYCAGLMKYGELHLTPLQSVVQIRPQFDFYNQKAAEDKDKKKKDKDEEEEEQEQEKPAPVSFRKQNGRFNSNQPPNFIKMFEDNEQWIKVNYVHDKDEYNDSLFQTYQKLINTEKHDRIDFEMTEAEYFDMLCPKVSEEPGAKTNHLLDTVPLDTIHQMTWKDQIKNILINAQVVTFSKIVHLLRTKEYIEDYQIIEELENIAILIRGRWVVKSELVSKDGSIIAARDYLLLLFSKSEIVSRKELCDETNLTPEEARELFVKVSDINHKDRAWYLKKEPDEDFVSRYPLVVNKFERYFSDREQLIKKKVKDYKTPATKIVYPENYRAGKNAKEQLHYLLHNLFNKYGVCSMQLIKQHLVQELESKEANLLGNLNLDKELPTELSNIALVLHNSYVLKSSGDPNIDKYRNVVIDLFKNKLGLKKEEIQGAVKSVVGSEIPRNIFSVILSEFTTKGKNIYILKVAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q6K9</accession>
    <name>A0A8J4Q6K9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003638</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000116">
        <property type="protein sequence ID" value="KAF2075071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q6K9"/>
    <dbReference type="OrthoDB" id="30292at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF162">
        <property type="entry name" value="ARRESTIN DOMAIN-CONTAINING PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="605" mass="69010" checksum="A0B16BF48ABC082C" modified="2022-05-25" version="1">MMDIRTLTLNIIEGKELIGADKGGKSSDPYVKFHVDGQTYKTEVKKETLSPIWNQIFQLPNINCDSTVFNFEVFDWDRIGSHDSLSGVTVPCSLFKQNALNGVKDQWLNLDKKGFIRVGFEFYPPYQTLNGAAPVPVNPIPTFNNVSISNNMGILPPPVHFPPLYLSTIPQDTKAEFILPNEVYFKKTYITGEPVDASLILYVSQPCVFVRSLYVSFRGKVTFNGKKTKDLVQDYRNLLHCVQTAPNQKTRLDRGKHIFPFQFFIPKECYSTYSMKGYDVSYKFVFNADIVNLPDIEIAKAINVVNIEDTIHRVTKSPINQDASKSPLTGGNISIKVKSPKNTFYPGEDIELDVFVENNSNKKIKKIDFTVHRTISEFGPTEYENSVQTTKQFFPKIKQKTSHSQNMVIELPSHLVRSIYQPKMIKIEYQIHAVLDIPKCVDLSIKIPINIVLPDPKIEPVTSALDEIASIPRYIKDWSIRNFNSWLLFKVKCPECVHNPIFYQYNLSGEELMTMDRNFLASNVLVGSGPRNQEIFTALCEEMDKILAVRNFLKDNQLAQYIPTFESETITFDLVKDLTLYDLGSLGLNIGDTKRFELLIKKLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q931</accession>
    <name>A0A8J4Q931_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA methyltransferase 112 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001590</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRM112 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000039">
        <property type="protein sequence ID" value="KAF2077081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q931"/>
    <dbReference type="OrthoDB" id="2187549at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070476">
        <property type="term" value="P:rRNA (guanine-N7)-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039127">
        <property type="entry name" value="Trm112"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005651">
        <property type="entry name" value="Trm112-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12773:SF0">
        <property type="entry name" value="MULTIFUNCTIONAL METHYLTRANSFERASE SUBUNIT TRM112-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12773">
        <property type="entry name" value="UPF0315 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03966">
        <property type="entry name" value="Trm112p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158997">
        <property type="entry name" value="Trm112p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="127" mass="14653" checksum="9650DC41FEEB0716" modified="2022-05-25" version="1">MKVLTHNMMACTKKQCMGKGFPLGVECTESITLEQEFNYEFVKNIIPKLDWKGIQLIGEKLAITIPPFTEESLLDQEFLKSLYHLLCNVKIITGSLICPNCQRVYPIEKGIPNMLLREDEIYQDVRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q9T6</accession>
    <name>A0A8J4Q9T6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_001084</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000024">
        <property type="protein sequence ID" value="KAF2077621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q9T6"/>
    <dbReference type="OrthoDB" id="266020at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009967">
        <property type="term" value="P:positive regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000383">
        <property type="entry name" value="Sex lethal, isoform D"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002343">
        <property type="entry name" value="Hud_Sxl_RNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050886">
        <property type="entry name" value="RNA-binding_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48024">
        <property type="entry name" value="GEO13361P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48024:SF56">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN A0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00961">
        <property type="entry name" value="HUDSXLRNA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="48"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="136"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="559"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="376"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="408"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="228"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="379"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="419"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="429"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="439"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="483"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="501"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2077621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="643" mass="72526" checksum="A04E4CE1F01ECE8D" modified="2022-05-25" version="1">MEISNFQQLSINDDNNNNDESDQNQNYDLNNNNNNNDESFYSFEKDQTNIFVKYLPNEFNDENLGALFSQFGKVLSSKVMIDPKGHSYGYGFVRFSRSEDSAAAIQAMDGYQFMNKKLLCRLSNLYTNLNIKNPSNNLFVKPLPPEITDEQLKILFLPFGEITECKVMVDHNGQSKLAGFVRFLNEADATKAIQAMNGVKVHNNSNPLVVKYADSEQQKSIRKQRKFLNYYQQALEQQQQQQHQHQQQQQQQQQQQTQGYYGYMDPYIYHVPYPHQYYNSSIPPTNNTSPHPPNIYQQPPPTYPIYPGNNNSYYLNSSIGSPHQHIQPTDHLNLSTGSLSGSGGMLPLIQAPPPSIPSPLLMESKHHQQPIIKLPYDPNNPTNNNNSNINNSYITPTPINNMVFTTTTTTTTTSTNSRPPKRNSKKSRNTQQIPQHLENNNNSHQPQPSSTTSTPLNSSSSSIEPLSPTSSTSTSSFGMSPKRNISSPLPHQNPKGNKTINNNNNNNNNNNNNNNNNNNNNNNNNNNSNSNSNNTTSTTPNTTTTNTAGTAKKSKSDSSNLFVFHLPPFVDNNILYQLFSQYGPLQSVRVIMDKETGENKGYGFVKFTSREDAVKSLKDMNGFQIGQKFLKVRFKTSQDKMIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UNL7</accession>
    <name>A0A8J4UNL7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010529</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001174">
        <property type="protein sequence ID" value="KAF2068146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UNL7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="506" mass="58025" checksum="1928FC1A02F791BC" modified="2022-05-25" version="1" fragment="single">MDDFVSLFNSSSSSLPYTVNSPLGCNSSKMVIAHRNDSVWNSDYYINCGNSILLRDFRNHSFVQKVYPDDTNFYKNSSLQLIAGNCYYHAWFESNPLRMVIRYNYDNLFIKSNFTIPSNPTNLVCESYTGCIATSPGFIHHYSFNYWPSNDRIDLYLSKTNDIFIRKHGIMDTKLYKIYFCRDTDDGFIIEAWEMYGYNVNDFLYSKEFTLAGSCKSMGYDYELGIVFVLTPKFLLAMDHQFDGLSKVTFDGIENVVSLNNIFNTGYRQKIDNTYSTANFNYLSITQSFGGSTSLRLIQYTSHYDQCKNGVYHDRCQNTTSILLNDTLYISLHGYFEKNSSMLSIEMGGIKHLIINYNSSLIQFVKTIFTPPRILFSINKIKYDFIVDNLYPMIIIGESVQQDGYIFIHGNKMASWYQYIPTIGNKTIPTNFINSTTIKLSPESTIDPNSFITLKINYQQELIDLSLSPFVKQTNPSTLSDKGQVITISGSFFGSAIVYLDVSELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQH6</accession>
    <name>A0A8J4UQH6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007997</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000458">
        <property type="protein sequence ID" value="KAF2070696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQH6"/>
    <dbReference type="OrthoDB" id="3881at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039732">
        <property type="entry name" value="Hub1/Ubl5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13042">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="2"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="73" mass="8392" checksum="A27DE2A7340063A8" modified="2022-05-25" version="1">MIEVVCNDRLGIKVRVKVNGDDTIGDLKKVLAAQIGIKPEKIRIQKSYIIYKDHITLDDCKYQTKNQLNSILY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4URG3</accession>
    <name>A0A8J4URG3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006509</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000304">
        <property type="protein sequence ID" value="KAF2072166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4URG3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2072166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1093" mass="128943" checksum="3231CB9FF486A018" modified="2022-05-25" version="1" fragment="single">NNNNNNNNNNNNNNKVTTTRQDRMNILLLNRLFKSKENSKLLDLFILESFKFNPIFLYQYLKFIKNVKNVESFQVEFMFKLIIHSFQQHFPLELIHTKTNDIIMDLFKYLIKFKTNCFQKFKNIIQNQPKAALYIINLLYKYLNNNSKEIDFQFFQEINFSIYSLSTCFFIENNNLSKDLFTILINQLLNNNNNNNNNNNNNNNNNNNNITYSIKSSISVLLDLSIAFPNQFSNTLVKNNFSNNLITILNVKLKSNESSQDRIFIIHSISFIINSFLSKENSNNDSKEILNLFQIINQCLESKSTVSRINSLICLSYLFKNEYFGDSLHLYIFQNIVLKQQQKEKQEQYISIDEKFHLLDFLNNSIEKISIESLESIYEHYLLPMVIESEIFKQESFNFQVINQHWLYNKLLIKCFILVSLKVSSNFPRPDYIQKILNNLDSLKINQPTLINNGGESKEEELEKKIFYYYKLIRFGCFLVNYSNTNIILPLMSYLFELNQKFNRITEQHFQISKSTNFQYPSLSLAFTSISFLTLIACIRSYGSYIKSKEIARDLFLFYSNILCEKLSCSQNLNIGSFFEQESLSDQILSISIESIDSGVICCLENNFLKWLTFFIFQEIFPLFGKEKKSYLLNILAIGFQIIRIFQSNVKINQWVLGDIVFGITCNKEDLEIKLDQKSGRPTKELNQVLFRVVIKDLVLLFKEKTFDRYIAFYLLDIIKSLVKSCSFKTDISFPDLYYELLVKLYKSNNLNYTKSLYIPIENGLFQEKLSNSSMNHCDLTTNIFFSFVQSIYDQGDTKESILAILKIFQIAFTSFVKSCLSPNQKSTKKRKRSQREDPQFLKSSLLEYKEDKDYRFINTDNLFLIVQIFIRIDLLLITEFEFIDLFKLNSNDSNTLNYSKQIILLYHLVENTFKLLKTIPCIEKYNQDLFPFTLKKIVNKFIDKLDQNIEILFELFEKSQWIFSIQDHSNNYLLLFDKIQKDCQLLFIKINSLLNFVQQNSLSSNRISIDKIILNCRNISKYFTNVEMEVKTNDLEHDIKKQGEEIVGKLKNNIKITSSYQIHDLNNEDIDENFCISQFKKLDRSELSYLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UT19</accession>
    <name>A0A8J4UT19_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000154">
        <property type="protein sequence ID" value="KAF2074296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UT19"/>
    <dbReference type="OrthoDB" id="21639at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051647">
        <property type="entry name" value="Mediator_comp_sub12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007:SF8">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="649"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="748"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="906"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="962"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1132"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="111"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="180"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="269"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="288"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="317"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="384"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="748"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="809"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="859"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="880"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="906"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="966"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="987"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1015"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1143"/>
            <end position="1168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1175" mass="130762" checksum="137D02F0A4A9F186" modified="2022-05-25" version="1">MDSKSHSSSSRNEMISLPPPPTPSPPLANTTAVLYQTDESEIMKSHKKQQIAYTLSPIPPQSTYPTTSQFGLHRNLHFQAPLQQPQPQLQQQKQSQNLEQDFGLEMKSYKKSSSSLSNNSINNINHSSNSSGSNSNNIYNNSHSEESITRGNTNILKYKSSSGKNQSVPISMLQSLSSKSPTLTTSFSSPALSSVTPLSPTRSIETIQSHHKSSLQLPDIKNDEKDLDAPIIGLPKIPFSSGGPSNHGAMGSSQDEGNIRKYHPYNKGSSSKSSSTLSSPPPSPSPSPQSSQQQASNSYNDNIPVSPSVSKSKSKSKSQQSQQSSQQLSQQYSQQSQSQQTQSQQQQSQQSQQSQQSLQSQSQQSQYQQSQQSSQESIQISSSPPQLTRSDTTSILHSKHRPARTWSMSDMAKYGADHFDFLNYNLDKDISPDNAHNKDETNFLFYRKVLAVLIGIKPKSILFTNQMESFFSGYFVLLDDKYQPIYEEPSSGGSSSSSSKIRVGEEEGVPLRYSKSKEFEGDKQVKRLGDFKDFRLGFRIIKQDTSKIYKVPLFQLHINQIILPCHLENNGRNNTNNANSFMFHNDPPTTCTNIQNQKWFWHFMLESVKILMLSCECNVTHSRFLKERHLFTPTDGEIAEHYYKNFERQNTAPNLTTPVSRSNSKSINPPTTQNTSSTQLLEYHNAFLADYQKSQGQMQKTLDSHSQSIDYLKDECKNIKSSISDLTNTMMNEFQGIKQLLNHLASSSNNNNNNSNSSASSSIGSGISSKNSNINGVSIGSRSSNNSNNSNNGKGESDSSDNDSGSSKYLNANNNGSSITTNLNNYDRRKEQKSSSALNTTLPHKEMQGVVSIRPSLTAPATDTQQKELSNSDSMDFENSQISTQQSTPTSSFTKSLSLQSSFSSSSLSSQSFSSSPPTPLIYKSPTNRHHQIDSLSNENFSEDQLSLSSAVFKRTPLSINHKDQNITSPTLGSYNTNQQKQYTTPSSSLSSCTNSILDSQSSTPSLSPSPTPTPTLTLLTPPISNTPFPTTNTSTITTHQSSDDESELLWRQNQLRDKHRKKVLNLKDSVLKTLNPTKFLKKREEYVNAVQKFNEIYANSPKTKMLKINDDFPQTTHDVFDYFEKHSLSTKKLTQTKRYKNRNNNNNNNNNNNNNNNNNNNNNNDNNGTDPKMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UU07</accession>
    <name>A0A8J4UU07_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MRH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010231</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000983">
        <property type="protein sequence ID" value="KAF2068443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UU07"/>
    <dbReference type="OrthoDB" id="23204at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23524:SF1">
        <property type="entry name" value="MRH DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23524">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G04850)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035197571" description="MRH domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1186" mass="132167" checksum="EE41963F8DCF98E3" modified="2022-05-25" version="1" fragment="single">MKQQQLFVVLFLLLCISGTAISALSCSQPPFRYELGRSCDMKNYGLIGTLYISSPQRYTFTLSPNADYTVDTDGYYYTLSPNINYTIEWKSYDNSLCNGKNYAFTPNPHFAISQPKCRFSQGTVTFVKPEGLDFLDVSCENAPCNVTFANKASYEYSYEDLEVECDFTPAIYELTDSYPNLKVQDSYYYNKTGSIQLIDKSKYTNWILTYDEDDTPIPESTPSSATWVGLQTAYYFLTLESSTCGIQRIPIKIDYTWPNYYIEYGTNATCPRNASVKINFDDPNIWDSPAVQILIDGHLITKGKSFYLGDFQDSQSSLQFNYHSDTFYNPISHPSMIGDIQYTVDPCTRLVTLYYNETLYSDIYAMDDDGNQLVITDKTFTIPSIYSYPTIESPCFFTSKTIRISQPVRPIYKIVKPQQFCGDTMDVVVLNYYEFESLEMGDVQHDGQGLFKNVIPNNSVLNFKNNGCYEFDTFSIPTLAYTFDQVEEIIEIISYPSCNNNGNVSYTVKDVKNNILSEIRYFAFTPSQSLALSTQFSENCSQSFSTQWLAPAVRKYVNTFPGTISVIKNTTCKYSNDAQIEFETNFLSVTSIVINGVIYEPSFSDAVVRFNGIPYGTYQITISSSDCVDYYETISIGTDNDWEFPAIVSPVTGDCSIPNGGISFDSSILAPIDALQSSLIANNLPSGYQTISFQLKNGTCSGSVNIFVPTASDSYATSKIYSQPSCSENHDGYVKFSVNDKNGNEFAPTGVELNSNSFTGNIFNLGEGSYNFIVKNSSCAWPVSVSMKAIEPEFTFKKIVDTFEKCEQKTFVSITPSSHNIYVDAITSGYSPLLQYSNNVYAYYPQTFSNTRQIFITYNQVCVKALRVEETTDLSGTIEWPTVTVPSVDCTDPSVQNSLAQVINPTNMMLYAGGFSVDHMYVPKYNFMSLFGFTANDKKSGCQKTFNLNTYNQGIVAKTITKSTCPGSIDGKIQIDVDPNSNMYNVYQVMDYGFDFLPVTSGSKLNSFSNITNIEYNLVRSFRNNPFCSVVENVNIVVDEPFVSLSSTGLCDASDTPIGDVGVVTNTLSITTTNVTYNLNGHFSTNPVFKGLSAGDYSSTVTIYNSVCRRVIQSNSVSVAKLPSVSVNVDVSTCMKAVINPTTNNIQHKYTIRDSTDKVVLESTSIGSFTFTPTSQDTYSVIASDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UU82</accession>
    <name>A0A8J4UU82_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000934">
        <property type="protein sequence ID" value="KAF2068548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UU82"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="214" mass="24115" checksum="C4E46BA9958F78DE" modified="2022-05-25" version="1" fragment="single">TLVQFNQQLEPGSLPPSLVSLKLVNYLKKLKPGIIPDTVQTLYFNHEKRKSPLKDLIPPSVTRLYSFYRGAIRVPDSVTELDIFFHKGTKIPDSVTTVKVFAYKTGVSMLTPGFIPPSVTTLVLQNIFKTKPSSIPPTVKLLKFIHYLPEVVDIPDHTTHVEIVHFDEYDSPFARLPPNITRLKLPNRHCLNPSAIPTSLRSLQIQGVYHLVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UUH4</accession>
    <name>A0A8J4UUH4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Dilute domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003217</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000098">
        <property type="protein sequence ID" value="KAF2075481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UUH4"/>
    <dbReference type="OrthoDB" id="47059at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048682">
        <property type="entry name" value="COG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048684">
        <property type="entry name" value="COG4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013167">
        <property type="entry name" value="COG4_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002710">
        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24016">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24016:SF20">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20662">
        <property type="entry name" value="COG4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08318">
        <property type="entry name" value="COG4_m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01132">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dilute" evidence="2">
        <location>
            <begin position="649"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="428"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="765" mass="89479" checksum="8A97C01AED589DCB" modified="2022-05-25" version="1">MMGGNDSNQQRQQQQQQQQQKQTSPQQKSQQQQPTDKKEDEFAEFDSFLKSMKVGNTTSSDMDILVLKVKDRVGKNEFSEALKILKQFVYISIEPITKTTIGSMSDSNTYNSLYEKYHIPSLNKIEELYQLTRSKTEETIRTNNQESLINVSMILSIVQQMKQRIIFRQQQQQQQQGNTSSMMLEEDEGLALYIQFQRKIILQESGPLIKEMIKVNQSLDGKDIKTKVKIFDNCLITLKKVLSSIVDKVSDALDVGEDQWKWNNVLESRFQTKLNEWNPSLLSLPLHNQSAIYLDQIYNIFLEKIKDTDLLTKMGDVNFQLEELITNLEYSQPIIDNVQSLLSVDEILNKISTMSEISHRYIGFIQENSGNQNIAELNNMKSRIQEIMTNYIGLESQYFQLSFSYAIYMDDLRKIVYNLIKQQINKGNQQQQQQSQQQHQHQNQLQNSQHNEKFNIQEITTSSMVDDIFFVFRKVIGRSISSLSSPTTCAILNLSTSNFDILYIPFLEKLLEFTYIDDFDKRRDYYLVILNDLYYTKEFMKQMQDQLKETTSHKFKNQDDVKMITLCIVGDGFVSLEKRINQMLKESIEKIVAILGSSLNQSIWPFRNINYEITDVEFENYEINDPFAMDFMNSLFNVLEPYKRKLAPQNFDLLVHLISVQIANILEGHIKQKKFNQLGGIQLSKDVRKIIEFLCNLTSEQNVRHKFTRLSQITHLLTLDKVTDVLEYWGQEGYIWKLDMLEIKLVLGRRSDFMMSNAIHNLKFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVW7</accession>
    <name>A0A8J4UVW7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">leucine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Leucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_009563</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000742">
        <property type="protein sequence ID" value="KAF2069120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVW7"/>
    <dbReference type="OrthoDB" id="15954at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07958">
        <property type="entry name" value="Anticodon_Ia_Leu_BEm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00812">
        <property type="entry name" value="LeuRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000003">
        <property type="entry name" value="Leucine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000011">
        <property type="entry name" value="Leucine--tRNA ligase chloroplastic/mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000100">
        <property type="entry name" value="probable leucine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002302">
        <property type="entry name" value="Leu-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025709">
        <property type="entry name" value="Leu_tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00396">
        <property type="entry name" value="leuS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740:SF2">
        <property type="entry name" value="LEUCINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740">
        <property type="entry name" value="LEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13603">
        <property type="entry name" value="tRNA-synt_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00985">
        <property type="entry name" value="TRNASYNTHLEU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methionyl/Leucyl tRNA synthetase" evidence="14">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Leucyl-tRNA synthetase editing" evidence="15">
        <location>
            <begin position="165"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="12">
        <location>
            <begin position="554"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="13">
        <location>
            <begin position="642"/>
            <end position="782"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF09334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF13603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2069120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="827" mass="95368" checksum="B50060781C47553D" modified="2022-05-25" version="1">MSDCISRLKRMQGYDVVHPMGWDAFGLPAENAAIDKQVSPSSWTLSNISSMKSQLKQLDFNFDWDRELSTCDKDYYRWTQDIFLRLYENGLAYRKSATVNWDPIDLTVLANEQVDAQGRSWRSNAIVEKKQMNQWFFKITSMADRLHDDLDTLPGWPEEIKNMQKEWIGRSHGHVMEFTFDDLSILSTIKVFTTRAETLYGVTFVSVSPKHPVIDQIKEIISPEKRQELEEYLKKVESTLQSNDPELMTFNTGLLLVPPSNDRSNHIKLVISNLVHADYGTGAVMGVPAHNRADYQVAQKENLPIISVLEPSNTDHGECYDETTGIMINSKDFNKQTIKQVIEEMEKKKWAIKTTKFRIHDWLISRQRYWGAPIPIIHCDKCGEVPVPRDQLPVVLPTEIEFTGKGNILNQLQDWKNVKCPCCNGNAVRETDTMDTFVDSSWYFLRFLDNKNYKEAFSREKVNKYMPVDIYIGGIEHAILHLLYSRFFTKFLKDQNMIDHSEPFKVLLAQGLVKSPTYRDEITNKPIHPKDIDFTNPKVPISNLTRNKINITIEKMSKSKLNGIDPSEMIEKYGSDTLKLYVLFKAPPQNSLEWDTEGIEGCKRWIKRVEILVEQFISNNSAKDILWFDRADPKMKKEILSILYESHSTIDRVTESIDKYTFNTGISALMTLSNTLSKLSELSKSTLEYYHALRILILLLSPFAPNTSQKLWNDLVSVENIKNREFLESDNVQSSGNSKLNDLYQQSWPIASKRALTRESCTLVVQFEGKTKGVIDMPSNLTQQNDILEFVKQHEKFSKKFTPTTMIDKIFVATTKTGYSFNIIFVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UW64</accession>
    <name>A0A8J4UW64_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ABC transporter C family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_009396</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000698">
        <property type="protein sequence ID" value="KAF2069283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UW64"/>
    <dbReference type="OrthoDB" id="29192at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031154">
        <property type="term" value="P:culmination involved in sorocarp development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18579">
        <property type="entry name" value="ABC_6TM_ABCC_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18580">
        <property type="entry name" value="ABC_6TM_ABCC_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000080">
        <property type="entry name" value="ABC transporter C family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002822">
        <property type="entry name" value="ABC transporter C family member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000010">
        <property type="entry name" value="Multidrug resistance-associated ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044746">
        <property type="entry name" value="ABCC_6TM_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044726">
        <property type="entry name" value="ABCC_6TM_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF172">
        <property type="entry name" value="ABC TRANSPORTER C FAMILY MEMBER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="93"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="178"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="302"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="742"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="783"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="863"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="63"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="432"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="747"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="674"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2069283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1143" mass="129492" checksum="32D5A01DA3C77FE5" modified="2022-05-25" version="1" fragment="single">MLWKGYFRGPLELSDISDLPSKLKVENSSRYLNSVQFNSKYSLVKHTYTHYCSRHLIVPTIKFISVVFSIITPLVLKYFIYYIQQENKSMAEGWGLCFALFLCCFIMNISQQYGYWYGMKVSLEIRGALISFCFKKMLLLSNTAKRKYNAGRILNLISIDVGAFQDFYWNIFVDILLFPFQILCLLVILCIIVGPAGLFGFVVMGLSIPLTTFLSRKMSNFLSISLRYGDERVQLTSELINGIRFLKQYAWEKVFLDKINQQRDLQMNYLYKRILCWIVNQAIIQSTSAFVLLTTFLVYSLLGHQLTASVAFTSLTIFVNLRRPMEMLPQNIQKFLQLLISSERLEEFFQSTELQTASFNIFDSPISPILKSAKPNTKDCDGQVEIVNGYFDWNDGAGGDNNTDNGTEIENSSIVVEYLPKIDLGEPADQNIELEPIVYDNSNSTTSSGLSTPPLKSTYGTFRNVNFAAPKGKLTIICGQVGSGKTSLVSALIGEIYKVNGTVTRPKTISYTTQQAFLVSASLRENILFGKPYDKERYAKVIYACSLNTDLLQLPGKDLTEIGERGVNLSGGQKQRISLARALYSDSECFILDEPLSAVDPEVGKHLFDHCIQGMMQGKTRILVTHQLQFIPSADHIVVIQDGNLIQGTYSELQQLGIDFESIMKTKKLNVEQQEEEKKLSKSSSKIPMKKVDDINPNDSIISDENDQSIIEKSKLLVKEDRNKGSIGVQTYKDYISAGGSLSFFIAIVLGLYFISQVIFQGSDYWLSVWTQRRVEPDPGDKFYLLIYFAFIMGFMCLLGIRYFLLSRYTYFASCNLHKQLLNNVTFASCQFFDQNPSGRIMNRFSKDIADIDTIVLEGFSDVMYCGSSVIVSIGMMIFLNPLICIPFIFLSIIYFFIQKVYVASSRELKRMESIARSPIYSHISENFSGLATIRAFDQNVRFIADMNTKINLNLRLFFYAFAVHRWVGTRLELISAITVLFSSLFSIYNTSMSPGTSGLAVTTALSITGLLNWSVRQYTELEVRMNSVERLKKYIDTPREGKRFTLEEDDNDTPLLDYRWPQHGGIEFKNVEIRYRKTADPTLKDISFTIKPNEKIGIVGRTGAGKSTIGVSLFRMVEASKGVISIDGVDISKIGLHDLRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWT2</accession>
    <name>A0A8J4UWT2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Clu domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_008611</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000548">
        <property type="protein sequence ID" value="KAF2070065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="626167at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15466">
        <property type="entry name" value="CLU-central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033646">
        <property type="entry name" value="CLU-central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025697">
        <property type="entry name" value="CLU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027523">
        <property type="entry name" value="CLU_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601:SF16">
        <property type="entry name" value="CLU DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT EIF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13236">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12807">
        <property type="entry name" value="eIF3_p135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51823">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Clu" evidence="4">
        <location>
            <begin position="649"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="1250"/>
            <end position="1283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="466"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="550"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1365"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="8"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="485"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="581"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1365"/>
            <end position="1457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1466"/>
            <end position="1487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1497"/>
            <end position="1521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2070065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1536" mass="173464" checksum="595B7A7C15D73A72" modified="2022-05-25" version="1">MISIFSVGSSTGGTNTPLSTSSSSSSNSNSQAQQQHQYQYCNSINDCIIQILNQFIEYFDYIFSRIYRYGGHPNSFFEHVDESRGQGFSPVHIYYGTKSSLMVLMFLLLAWYVIRAERRNPRVKTYIRNRWKKPPLLNNLLRKNRLRNSKSSMPVYEMDDYDQFDSSVPYDYNYIESAPRKRKGIRSRAIGIASNGGGSGASTPNRQQLLLKDSTSSIKTTYTAADEDSYFDEKQLDTDTLSLHSFSSTLNNGNSSNNSSNKLMESFDTNLSRSMDEETMGEEDDQMDLNVSIEHERRKKLNASIKEQANRKKRNQILSEPLLEDVELNEKQNLPPPFKKITPKYSKPLFNYSYTSSALFNNNNNIFNSHSNNNNNNINSNLLVPPPTFFSAKVDTANYIPTRTFGYNYKDTGGGNIHVDIVENPILSTTRDIFNSSSSITSPSAATISLDDYLKKKEKENSIINNTTILSPGSPSPMLRSSMRGVNNQQHPGQQQQAPVAPNRSKLLESNYKEDDLSNISLRFASSNISVNREGGDSDSVVDDADEDYYNDQVDDEYDHNVETIDNTDDADDEKGFPANEIKKMNKKENDKDNELIDQLYKGEKKKQDKQQQQQDKQENESDESVPKVQVTNIAPQSVDSILTSIYSTEDEEDYCGSDIDGEDFSSVNIDYNERYQEIMEALRNIDCNSLQSDKIKVNSQFIALTQDFVYASQTYGKVIISERYLPNHEKTIPPIDIGGLAGGDKYIVHGILFKFAIDKENFYGSDYAAMCVGGHELKGLINVFNCSQTHISLPLMALVDYRGFRVIAMSILPVDKDSIYYGSNDYGVNIHNKDVYLSEKVKSISSILNLKSHHCGEQKTFLHSPADLEGHRGFDGRYYLLDFARLCPPAAPSKDIKMGHLFRLLRPEFVRNYKTPLCSDSFSRFIRGHNENEHNTEALEATQYLLNTTIPELGEELNDVEINIANIYTFPITERLHRSGVNVRYLGYIRQHCQSLDMKSLIFIEMCARVVKQQLRQKLRFKMKQAKIPLEAPYRRLIVAYLNRLLGISDKSDLFWNTKMKRHLLRKFDRGLFEDEIMGNSLILGLSSFSDHNIDGKYLLFKRIQKMTGLKFTNRINTEFESQPNCWLQRGEHPLDINDLDEIGIRVKYVPFMNVAMGYLFKVKGEMLFASDPIAATRFFHMALDKLDIALETDPNNTDTLCTIGEVNSLLGMGDQQGLSEVQLSIKNPYISKAVDYFQRSIFVNPNHTMSLFRFAQLLERCGEYDDAETYYLASLATNPNNIACLQEYGNFLQTARNDFTTAELFFMRGSQNHYYLHTQRERQDNPINIRGSSGLLRLKQTQDSNSSSSTFSFINNNSNNSSMINHPLHSIPSSSSSSSSHSSSENYNSSNSNSNSNSNSNSNSNSNSNSNSNSNSNSNSNSNSNSNSNSTSISGSNNTNTNANNSISSGSGNSIQHHHHHHQISSSGKSNSSSNLINNSNESASTIFNNHRRSGSNGSIDTISPIKMNSSKDSNNSPPTKIPDSPFQPRKFMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UX20</accession>
    <name>A0A8J4UX20_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Adenylate kinase active site lid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_008740</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the adenylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000569">
        <property type="protein sequence ID" value="KAF2069943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UX20"/>
    <dbReference type="OrthoDB" id="439792at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000106">
        <property type="entry name" value="Adenylate kinase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006259">
        <property type="entry name" value="Adenyl_kin_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007862">
        <property type="entry name" value="Adenylate_kinase_lid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036193">
        <property type="entry name" value="ADK_active_lid_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01351">
        <property type="entry name" value="adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05191">
        <property type="entry name" value="ADK_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57774">
        <property type="entry name" value="Microbial and mitochondrial ADK, insert 'zinc finger' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Adenylate kinase active site lid" evidence="6">
        <location>
            <begin position="154"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2069943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="246" mass="27884" checksum="178A6AF5EEDAEE74" modified="2022-05-25" version="1">MLKCIHKIAICNSNKITYTSSRYYSTQSSSLRMTIIGAPGSGKGTQSTKLKRDYGVVPISTGQILREAAEEDTPFGKEIAKKLKTGDLISNDIMAEIIQKVLNNQNNWLLDGYPRNPEQAEHLDKVLKEKDIPLTVALYLDVPENILQERIQDRWVHPKSGRVYNSVFSPPKVKGIDDVTGEPLVKRSDDNDEKVFQNRIKTFKESTLPLLKYYEKQGILYTISSPTSDEGYIKIKKVLDQVVHKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXA0</accession>
    <name>A0A8J4UXA0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008028</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000463">
        <property type="protein sequence ID" value="KAF2070650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXA0"/>
    <dbReference type="OrthoDB" id="16817at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="418"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="434"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="456"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="475" mass="53336" checksum="D4050CD6D2284BE4" modified="2022-05-25" version="1">MVASADLNISIEKTSHGTQQIPSHLFNINMEDFMLLSNSQISYLYRSIKGSKTTMVYAYDGTRRSWLINENNSQFGLPSKDKTVLIDYAEYSKTAIHNLLYQLVMMFQHGVDTIVYPMWFCTLENRGPEYLPKFIQYLWGLKALLDNPELIEMYNANGIRVIFYGEFRELLQRGNDPELLRVFEKIMDQTKHNTQKTVLLGTNIQEPSDVIIKETFDFYSKHGRKPSKNDLVKQHYGVDVSDVNFYLGFDRFSTDGRPILISDKGAEDLYYSVSPHSFLSTTQFRKVLYDHLFLRSVSNAKEYTLTAMDIEMMKIFYQNNSNSIMGVGAVNPHGRYWYPTPQVMVSGGSSNSGGSSSSSVPSLLIHLMGQKAMNNSNTGNDSQMTSPPESPCNISRKNSFNSNSSASQFFNNNSNSASPHVNCHGHSCHSPTKSVSLPGSPRGSPSLGGLSNSPPTNKNLSPSLLNRMQPFLQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYI0</accession>
    <name>A0A8J4UYI0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Seipin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_005896</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000247">
        <property type="protein sequence ID" value="KAF2072795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYI0"/>
    <dbReference type="OrthoDB" id="3990054at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140042">
        <property type="term" value="P:lipid droplet formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23995">
        <property type="entry name" value="Seipin_BSCL2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009617">
        <property type="entry name" value="Seipin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21212">
        <property type="entry name" value="BERNARDINELLI-SEIP CONGENITAL LIPODYSTROPHY 2 HOMOLOG BSCL2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21212:SF0">
        <property type="entry name" value="SEIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06775">
        <property type="entry name" value="Seipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="251"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="295"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="371"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="419"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="371"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="422"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="443"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="460"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="478"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="511"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="563"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="581"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="605"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2072795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="621" mass="70796" checksum="9AC31997787A9744" modified="2022-05-25" version="1">MESFISRSIKWGLELTYSIVSFILRIWLVPINSVLEWSMDRIKPIFEPLIQFVKDHKSKMFNAIIYSIVGLITISFIFFLSAISYYFLYSYTVPKVTTIQPLYFDLSNKKMTANTEVAVEFQRKKLYNIFLELEVPESPKNEDIGMFMACMDLDNQDKWSPQKIHHSCRPAILKYSSPFLKGIKSFIMTIPYLFGAYEEKQYISIPMVENLLSTRFYQSISATVNILKPEIQFYKAQLVFMAQLSGLDYYLYYYPIFSAVIGISIIFSFYMSCTVFFVVSLYLFRYFRTQPELEVVSSEEEDEEEEEEEEEGEEQVEGLVGDELFGGSPKRPSWEKESNILPHSSSDDEEIGSSSSSSRLIRKRIFNNNNNNLDDINSTSATKSSSPSTSFSKYRWDEDEEESSLKEIIAFEQKKYEKQLASSLNSTPSSLPSSSKKDTGDNTTTTTTTTTTSLLHSHTPTISPLSSVYSTPKKQKSQFDSSSSLVASPLSSSVTSTTTTTATTSTLVLETKQEKEQEKNIQDSQEVPRYESQENLVEDEPCSNVDSTATTTTTKEEEKEQEQKEEEVEDEEEEERDNIFGSDQESGSGTPQGNSGFVADEWVKISTNEQSLSDSNLRKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYZ8</accession>
    <name>A0A8J4UYZ8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Guanylyl cyclase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006429</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000296">
        <property type="protein sequence ID" value="KAF2072253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYZ8"/>
    <dbReference type="OrthoDB" id="206796at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018616">
        <property type="entry name" value="GUCD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31400">
        <property type="entry name" value="GUANYLYL CYCLASE DOMAIN CONTAINING PROTEIN 1 GUCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31400:SF1">
        <property type="entry name" value="PROTEIN GUCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09778">
        <property type="entry name" value="Guanylate_cyc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2072253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="27613" checksum="431A6EF045D250D9" modified="2022-05-25" version="1">MFIINDIPHFKQNLDWECGLTCLKSIIIWSNSSKGEANNSNNGNDIDDTELSGMLNTQSIWSIDLSHVLKCYDIKHTFVTNSPFARDSHAQLSFYKDDWDEDSARVENLFRETMMNNPDNLSIISSPLPITAIIEHLRSLQPIILLVDSCCLCCSSCVKPSRSTFHYQSTNTTTNDNDDSIFMGHFIILIGFDDILDQIIYIDPSSNNDRCVMDVKDIDRARLKPGTDMDTIFIYKNDHQQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZS6</accession>
    <name>A0A8J4UZS6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010421</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001102">
        <property type="protein sequence ID" value="KAF2068252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZS6"/>
    <dbReference type="OrthoDB" id="18358at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="149" mass="16556" checksum="AABD81C088906DEF" modified="2022-05-25" version="1" fragment="single">ALESGNTTTSNSDYIKLQVDDHSLYGRFIKRGVIDGRIVSVTNNLLPNYNSESNQFNNVQSYIGIGIQYYHELVQIDPDFSVLVDQRPAVDSVNSVCSSKSKSKISKAQLAGIIIGSVAFAAIIVTMTAYVLYQRKERVTFENKLKTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZS7</accession>
    <name>A0A8J4UZS7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">adenosine deaminase</fullName>
            <ecNumber evidence="3">3.5.4.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_005192</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.4" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000201">
        <property type="protein sequence ID" value="KAF2073483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZS7"/>
    <dbReference type="OrthoDB" id="272271at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004000">
        <property type="term" value="F:adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006154">
        <property type="term" value="P:adenosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043103">
        <property type="term" value="P:hypoxanthine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046103">
        <property type="term" value="P:inosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000091">
        <property type="entry name" value="Probable adenosine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001365">
        <property type="entry name" value="A_deaminase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006330">
        <property type="entry name" value="Ado/ade_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01430">
        <property type="entry name" value="aden_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409">
        <property type="entry name" value="ADENOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409:SF43">
        <property type="entry name" value="ADENOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00962">
        <property type="entry name" value="A_deaminase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Adenosine deaminase" evidence="7">
        <location>
            <begin position="32"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Adenosine deaminase" evidence="7">
        <location>
            <begin position="491"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2073483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="778" mass="88090" checksum="09618916CA405310" modified="2022-05-25" version="1">MIEIENNEPVNLTATTASKGSFLPLDILRKLPKAELHQHLDGSINVSTIIELAKEQNIELPSYDPEVLSTYVLKDKDCNGLVHFLEAFSITLKVMQKPYAITRVFYEVCENLVKDGVSYVEIRFSPILHVEQGLSLSEVMNAVCDGLALAEFKLPIKARIIVCGLRHLSPNVTKDLAEIAWRYRHKGVAGFDLAGPEDGFSSKYHKQAFDIVRDKALNCTIHSGEDSNYMSVFDSIICGAHRIGHGIAVQQNQDLLNHMINRRIPIECCLTSNLQIKALKHYKDHPIRKYFDQNAIVTLCCDNPTMSNITLSGEFSVAIENFDFNIEEVLRLIDYSFASAFIEAPMKSTLRKEAVIKSIRIFKEHGYDLDPIVKNKNYYFHETGIDIIHELSVIEKINSQFTLGKCLSPIHSPVTMEFLKAMPKADLQSRFDGSVSLSTCWNELQLIKDDKDFKKLFPINIDTFTSFDKFRETIQNPNHSTGSIALSKDIMNALLQNEGQLERGFDDIIKTAIDDNVKYIEIAFRPTSHTKINLTKEQVLNIIIEKKNEWENTGKIKIGLILFSSSVSDDPIECLSNAKVAIQNKSNGIVGFGIFGTETISPSEIKHFSQTFDLLKRHHFNLVQNSGTSDIGSLVSTIHQAGAARLSGAFQLHKYPRLMSYVGNYRIPVEISLTKKLKSFTKDLSFTTPIRHLLDNNVPVIICSFRSSLYSFGRTEMLYEIVKNAQLDLEHVVRLFKNPFSFNFQNLQERTKLVNLFNETSIQVLQNHKITSQNLIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZZ2</accession>
    <name>A0A8J4UZZ2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">VPS9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_010167</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000954">
        <property type="protein sequence ID" value="KAF2068507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZZ2"/>
    <dbReference type="OrthoDB" id="1163311at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.80">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037191">
        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24121">
        <property type="entry name" value="NO MECHANORECEPTOR POTENTIAL C, ISOFORM D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24121:SF23">
        <property type="entry name" value="NO MECHANORECEPTOR POTENTIAL C, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00167">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109993">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="290"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="VPS9" evidence="3">
        <location>
            <begin position="515"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="766"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="99"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="769"/>
            <end position="814"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2068507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="886" mass="99865" checksum="74DFAEBB529A5B1D" modified="2022-05-25" version="1">MENKGSPEISGIGGSHPALSSFLMDETVSDTSSNSSDYVQQQSQQAPTTQTLMLPPASIPPTTTAATTNSINMKGSLGGIGVIGGSLSVSQGSIINSKSSSSGNLMGGSSSYKDKDTGEKKPRTKLETMITSLEAGNMIFFGNSLKTKPIIPLTQILAQKNTFLHIACLSLRMVSVNFLLEKTTTQLVNCQNDKLKTPLYNCCEKGFFKAALSLLDYGASPLIANYQKWTPLHRLCSLEMKKNLYPYTPDSLIGYSPEAQLELAQKMFTVLEKSKVENSISNFIALKTNKGQTPLHISILSNNVDLSKYLLTHSTRESLKIKDDNGNTVLHLCSYRNNPKMINIAETILLTLSESLDGEAMQDYIDDINNLMATALQVSMKMANTTLNRLIVKYRAQAESKVLEEYFKSIESIDSSELLKISLETLAYNDDFKSTAFGKQFRRIVILIKQHYAINMSHQNSFVLLKRAKQTIRRYFDWLDSKIDHDHYEYLTVYREMLFTQIYDTIIHLYHETNKSKDLSFQQRVNMFKEISYKELDISEESWDQNVNILPKAMEILNSLPTKKTPAEKIETLNNATMQIQRNDANDLTPMFMYLLIKSQVNEIQSEFQFMDDFKDTPEQEQYLLLLQGSLDYLETLNYTLRNGHNQIMSLNSIRETTIDNILSLIDEFQYQASGADQVEDIDEGIQMTDQIILLQMLFTKLSNRTTNDPVYLPMKWSNPVMEAHHFKAVIERLGLKLDLKESSLIKNNSDNNSLQQSTTPSLECIQENNNCNSSPTTSPLLGGDSSPSLLSPSINNNHNNNNNLIPQCNINNNTSNEAPLVTRDSTNQSSSVATKEYPAGYICQKRGEVCVILEHPYPQMVYQIIEEKMRESVLPQMKNNKGKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0J5</accession>
    <name>A0A8J4V0J5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histone RNA hairpin-binding protein RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_009383</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SLBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000694">
        <property type="protein sequence ID" value="KAF2069302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0J5"/>
    <dbReference type="OrthoDB" id="265795at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071204">
        <property type="term" value="C:histone pre-mRNA 3'end processing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071207">
        <property type="term" value="F:histone pre-mRNA stem-loop binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006398">
        <property type="term" value="P:mRNA 3'-end processing by stem-loop binding and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1120:FF:000001">
        <property type="entry name" value="Histone RNA hairpin-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1120">
        <property type="entry name" value="Histone RNA hairpin-binding protein RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026502">
        <property type="entry name" value="SLBP1/SLBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029344">
        <property type="entry name" value="SLBP_RNA_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038294">
        <property type="entry name" value="SLBP_RNA_bind_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17408">
        <property type="entry name" value="HISTONE RNA HAIRPIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17408:SF9">
        <property type="entry name" value="HISTONE RNA HAIRPIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15247">
        <property type="entry name" value="SLBP_RNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Histone RNA hairpin-binding protein RNA-binding" evidence="4">
        <location>
            <begin position="106"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="27"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="122"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="65"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="181"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF15247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2069302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="278" mass="32119" checksum="BB8C21927B996503" modified="2022-05-25" version="1">MTENKTTTSTSTLGIYKDVGITNEYQSYRNSNNSNNIKSSGGNVYRNNTGYSRDRYSKPYDRNSHNNNHNNNNNNNKSNHSSSHENKRECTLKSLPNILKIQNESDPVKLKARQKQIDYGKNTIGYDNYTSQVPKHTRAKEHPKTPDRNQKCSRRSWLGQIKKWRRELHKYDPNGGKDDENFSDQDDDDDDDDERDRKHKKSTSSMNNNDDDKSVNNLEISIESKVIGNSNVEEINQKQQEQEEIEEIEEFDPEENQDLLNLLLQNSSTSSTVSTPPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1L7</accession>
    <name>A0A8J4V1L7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003111</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000094">
        <property type="protein sequence ID" value="KAF2075568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1L7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="112" mass="12022" checksum="73F913BEE046942F" modified="2022-05-25" version="1">MPSSEIKVASSKPQSSGFKVLNLKECKTKKMQVANLAFIIGYGIVCPPSALIVGGLVLRNHMRNKKSKKRGHLLMDNNIDNQDEEELLSTPSSDSGSDSGDSESSCSTSLHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1P9</accession>
    <name>A0A8J4V1P9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_001343</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DOCK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000031">
        <property type="protein sequence ID" value="KAF2077340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="18896at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08679">
        <property type="entry name" value="C2_DOCK180_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11684">
        <property type="entry name" value="DHR2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000487">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.350">
        <property type="entry name" value="Dedicator of cytokinesis N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027007">
        <property type="entry name" value="C2_DOCK-type_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043161">
        <property type="entry name" value="DOCK_C_lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043162">
        <property type="entry name" value="DOCK_C_lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032376">
        <property type="entry name" value="DOCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042455">
        <property type="entry name" value="DOCK_N_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027357">
        <property type="entry name" value="DOCKER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046770">
        <property type="entry name" value="DOCKER_Lobe_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046773">
        <property type="entry name" value="DOCKER_Lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056372">
        <property type="entry name" value="TPR_DOCK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653:SF2">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20422">
        <property type="entry name" value="DHR-2_Lobe_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20421">
        <property type="entry name" value="DHR-2_Lobe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14429">
        <property type="entry name" value="DOCK-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16172">
        <property type="entry name" value="DOCK_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23554">
        <property type="entry name" value="TPR_DOCK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51650">
        <property type="entry name" value="C2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51651">
        <property type="entry name" value="DOCKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="8">
        <location>
            <begin position="25"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="C2 DOCK-type" evidence="9">
        <location>
            <begin position="808"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="domain" description="DOCKER" evidence="10">
        <location>
            <begin position="1703"/>
            <end position="2129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="262"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="431"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="530"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="670"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1457"/>
            <end position="1476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="262"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="274"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="335"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="368"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="392"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="432"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="452"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="504"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="548"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="670"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2077340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2129" mass="237925" checksum="7977614F82FA026E" modified="2022-05-25" version="1">MSTPTTSSTSTQSSRLSRKLDEWINISKTGIVLYPFNATHKFQLPLSVGDFVHIHAQTSNEQTGWYSGASVTTQESGIFPQSYVVILPDQANVDDQSTLPNHIPICSKLSLSNEEMSNSSLVNEITMTIVEWATLLKTYSQNKQYNDFKILSNRITTLVQFRNQLLAFSSPKEITFEFRDKILNVIDDGRRETGMTIVLRKEISNSYSSTQSSSLSSTPTGGRGSVILLPTVATDLILADAENTGVFTLCTMYQEQKMLESQRQEYSKSRDSTNYNNDLNILNNDLETSSTGSGSNININNSSNSNSNNNSTSNGNTPSSINSGGVSPMSNVTLSSNQNSPASGSSSPNQSQSSSPSSSNININSPQTNGNNSLASMSSKDINQFSSSPGGSSFLVNNSNNSNSTSSSPSQGKLSKLPSSMNFLSSVRLKNKQPSSLSMSGGMQQPPLTPTSSGSSSNLNSNLSNVQNNSNNNSSNSNSNSSNGSLNSPSSSMVSQSYIAVSQSPSGNGNGSNLNLSSLNLNSPMILSNSSSGSGIKNKQAPLKKGTAGSTSSRSFLNHSSPSIGGANGAGSGAYGTDQVAVRQMSQILVSLNNFLYSSGEFIDIVLSIYNKNENKFISEPYCGVVPREGIFVEPENPQERIRTIFRDLDSKDMNSELYLVVKLYRKTNSVSSKDPNSSPTLTKSNVGGPGLTQQSLQQQLSANSKLFRKYVGCGVKKLDLSNEGLSEVLITLYTTTNENGFSNLHESLINEQSSLYEPITRAKGVVFSFQLFHGDYQQFISEHTDYRSITTCLKVHLPEVILPGSERNDMYIQLEEGDFSDKNIEVTMQIKTETGLLLHDAIKWANGAPEQSEFKTPLISSTLCKWSEIVKVWMAAKTFEKAHILFLVRQCSEKKDKERTLVAFGYLKFLSDDGAVIKDGSHTVVLHKLGSGSEEIPVSSYINGVVDPSQSGKSSSKKQDTIKVRTSFVSTCLTQNPLVVNLSRWQSFPGDLSSLIKDITFLGQHDLIRNLQEIFYNFLAILDQQLNDSPLSMEIFRSFVFIIGVLVDSRTTNYRPALDLYVGKYFGVPLSSSGSGNNHVGISAHTHLLRSVVKNLENFQDAANASRISSSLKALEYIFKMVVASKTKFTNKETVGNESYQTNLKNVVDILCEIMVSNAGSLIGAQTIALKNFESMFSDLKRFFTVEEMGIIALKFMKSIQHLEKNKTFNLLKLKLLSSFINGPMMLNKDMRKQLHPIVFQLLQFHFGKSIEETDMCLTILGLIVDIMITKSELRGKDKDPWLMEMLTFFVPILELLLLINSNNTNNNNSTSMINVNSINNGSPLVNNVINQAKRRSSSSVSLNGASTSISLASMVSSMTEDNINDLRIKIYAFILGSSRLSGVQHWEMINKSVTPTNKTFFLDLYESLQLLFETPKFPSDFWTFTVFQLKTILRMTRILEDVIFYQPTLVGSNDSNGGSANTGGGVSQQQQQQLQQTGGKSSLPQFEFELWKSFFLMTSSYLNCKDLQLESVNPAKAVFLKNRCGDVRIEMARVFERVWSAVPVKERSSFIPHLIHQIIKLSISDTLDAKRVATKIFYDMLESEIIQTEAYSELFYRTIESLLEIGAERSIDKTWPLVTRSHGVGLEGKFPFTLRSFSTFFNPQCMSLVLNKSTDQIKKKSEVFIQEINHFLTLITTFMGDIKNRDEEEIFASTTKLLLYLQEHKRFTHFVRFVHMCSKRHYSNGNYIEAAVTLMLHASLYQWDHNKVVNSIQNEFVSFPEQKESERKEILYKEILACYNKGKAWDRAIPLLKELIHHFTMNICDMNSAATYLRQQGTFYQKINESSDPIFEEYFRVGYYGKKFPLSIQNKEFIYKGNEFERLSDFIQKTLERWPKAELLKATDIPAKTIQDADAQYLLITSVNVSNINEIQKRSSVANAAASADSFSNKKRVPHRVQQFNARNRVNVFLYSKPFKKNTNANTPKSQNEFEDLWIMNLYFVCENSFPCTERRSCIVERKQVELSPIENALNSIVQKNDELQSRIDKYQANPQETIHPLTMLLNGIIDASVNGGVSRYDQFLSNEYLKQYPEYKNFAELLKSAMDQQLVVVEQGLKLHATLRPPEMAAMQDKLENFFNQMKIARQKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V206</accession>
    <name>A0A8J4V206_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial glycoprotein family member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000932</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000020">
        <property type="protein sequence ID" value="KAF2077745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V206"/>
    <dbReference type="OrthoDB" id="278212at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.280.10">
        <property type="entry name" value="Mitochondrial glycoprotein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003428">
        <property type="entry name" value="MAM33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036561">
        <property type="entry name" value="MAM33_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826">
        <property type="entry name" value="COMPLEMENT COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826:SF1">
        <property type="entry name" value="COMPLEMENT COMPONENT 1 Q SUBCOMPONENT-BINDING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02330">
        <property type="entry name" value="MAM33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54529">
        <property type="entry name" value="Mitochondrial glycoprotein MAM33-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="319" mass="36035" checksum="960D3E328E602213" modified="2022-05-25" version="1">MSALGRNIGNFVLTFSAKRTSFATANGLKSGLLSCCGSTYTPLPKDNHHHHRAHIQVNSSKSGLKTDLFNANNNTFDYLSVRNYGTNNLQVAPTPKSLLGSICESEINDFKTIIPEGNDIEAFLEESGFELTKEKETAFLKKKFADGTQITVKFDLMEQNDPFQEDDEFGDMDKEDQDEDQEEDQEEEEQEEQDEEQEQDEDEDAENEDGEDGEQPHEHSFEVQLSKANGENKLTFGCFAAQDGNYTISGFYKGGFEDIVNPVDIGGTSEEFQTNILLLLEQYGVNERLSFFIHDYIHSKKLNDYIDSFQALKDFASEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4M6</accession>
    <name>A0A8J4V4M6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004985</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000188">
        <property type="protein sequence ID" value="KAF2073697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4M6"/>
    <dbReference type="OrthoDB" id="10545677at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="210" mass="23576" checksum="5CD4FA2FFF8E3CA5" modified="2022-05-25" version="1">MSYNFLQSDLYQHSSCPSLGVLVGSPPNTPNNIYKEKKINRICIGSQKKTDQNNNQNNNYNGTKNSKNHSLWRFSLPSNYKNQTWVMGSINDKKSIKAPLLPSSSTFTSSESNKNNNIYSNGATVINLDDILEPRPPAKPTKIEEMVKGKFKVKLGKLLNNEEMLKQGKAIGKGASIYEDNLIKTQKRVRKHINSLPSHKINSNTIIIVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5C3</accession>
    <name>A0A8J4V5C3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000340">
        <property type="protein sequence ID" value="KAF2071774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5C3"/>
    <dbReference type="OrthoDB" id="17995at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="563"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="563"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="673" mass="75634" checksum="39CCB7827781289A" modified="2022-05-25" version="1">MTDLNSAQIEIPFKVYQEVKQGLEEISTHAFISNSYYKGVCEESFVRLSCEYTIRIYDDQSNAPSNNNNNNKKKNIVKKYSVQLMSQKATIEKSVILFSNNSNKNEDMVDASTKGACLLTNNGFYNLYTSAAGRYLVKLELLVPYLNNKNTGMELTIPQSTNNSVVFQVNQPNSNIKVFNSFPDVDSHQEWLSKHEKESSSTSLVFVKLPTDTHLKIQWTAIEESKKTLESSKTQTVSIKPNIVVQQHTLCSLGEGLLQLKTSFIYKIIAGIISQFNIEIENNISIVNVEGKAIKKWDISNENGGKRILKIDLDYGVENSYELKVFAEYSMGDTTGEVFVSPMICKGEEISRQRGYLGVEARTNIEISDIGNDGLSVADVTEIPKDLQSMAGHPILLSYKFLEPRYLLNLRVKKNSDCSVLLSICEEAHFITTISFSGKNIHQMILSIKNTQQQFLRVHVPFQYEVWSTMMGSTPIKPSLGEGFLMVPLLKPGNLNTDAPVKIEIVLLEKDCAPLSTKKGKLEFIIPKLDLPLRAAFYTLYLPESFSASNFLGNLKNVRHFSATPPSASSPTDDNNNFSNNNDNMIMPQQRKRSSYDYDDDSDGGYESINSSYFSKVAKSGNSMKGKRRAGVIPVKINMPTTSNQLKFEQILVDKDLSVSFDYKVKEVKPRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5Y7</accession>
    <name>A0A8J4V5Y7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Histone-lysine N-methyltransferase, H3 lysine-79 specific</fullName>
            <ecNumber evidence="2 11">2.1.1.360</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 11">Histone H3-K79 methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_000191</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Histone methyltransferase that specifically trimethylates histone H3 to form H3K79me3. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-lysyl(79)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(79)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60328"/>
            <dbReference type="Rhea" id="RHEA-COMP:15549"/>
            <dbReference type="Rhea" id="RHEA-COMP:15552"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.360"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="11">In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.360" evidence="2 11"/>
    <dbReference type="EMBL" id="AJWJ01000003">
        <property type="protein sequence ID" value="KAF2078568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5Y7"/>
    <dbReference type="OrthoDB" id="443402at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140956">
        <property type="term" value="F:histone H3K79 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025789">
        <property type="entry name" value="DOT1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030445">
        <property type="entry name" value="H3-K79_meTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21451">
        <property type="entry name" value="HISTONE H3 METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21451:SF0">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE, H3 LYSINE-79 SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08123">
        <property type="entry name" value="DOT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51569">
        <property type="entry name" value="DOT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DOT1" evidence="13">
        <location>
            <begin position="538"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="83"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="431"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="132"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="151"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="185"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="205"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="254"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="268"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="287"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="304"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="335"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="365"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="440"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU271113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2078568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="882" mass="102115" checksum="BB2ACA2C10502244" modified="2022-05-25" version="1">MTSPYRLNDKDKFHEQKDLEGPDDDLNQVYVISDDEGGVVVLIDSDSDEDIIITPSRKYDLNKEIKKLAQIFPSKEKDGSIILVDNSHSSPFKTKTKSTTTTTTTTFTHNNNESNGGSSNGIAKKKIDSVDSDSSSNSNNNNNEKYDNNHSKNNNNKNKNNLNKDDDSDPDLENENSFGLKNAISKRDHFNETSKHKLSSIESSETTNKKTNGTENRIELKLVNEQQDKEQDEKRNKEQDEEQDEEKLKAQRKIDKKNRRKNKKKEKKRLEREGKEKDLVTKDNDSDVNNSSKSNLNSHNNNSIRKEKEKEKDKSENGKDKIVNSTDEKMTDINEKEEEEEEEDNGIRVNKKRKEKGFLKNSWLDSDESSSEDEESKRPAKKHILIKRSSPLSSKKILIEKNPLLIEQKQDKSLIKQTITDENSIIIKNKEIQDFNGSKDDEEEQSEESEMDDDDEELIERQRYLDIKDPLFKKLAKELKKSNSLFKTIGEIVGDCGIKISGGNIEIITNERSKVRFKRGNQILMQSVEFRKTILDNSHVDKTTGVHRSPAEMMEHIESSSLTWGNIEFECQAFWKSCAKCNPRLDYERALSKAGGFAMERTSRVVLNDRGIRTEFETLYTIQQQRQMVKSRKLRCYPNGPRVMNYFEFLELNILLREEIPHERILKNYQMGKEAYGEVMPKLVNQWIMLGLIKPTDIFCDIGCGIGNILFQVAAMVGCRVIGVELRPDLFAISQRMLAKYKRISKWKQLDSCTQNIEIYNVDIASSTLDFNLYDPTLFFMHNTCFPTELEVKIMELMKQNSQPEAKVITINTLCPRYKPSDRGAKPWGIFKTPFERYEMEEDSLSWKPSKDCHFWSFTLDEELVIRKKNQENQESQENDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5Z0</accession>
    <name>A0A8J4V5Z0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006423</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000295">
        <property type="protein sequence ID" value="KAF2072269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="21646at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053019">
        <property type="entry name" value="GATA_zinc_finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23353:SF23">
        <property type="entry name" value="PROTEIN HAIRLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23353">
        <property type="entry name" value="RAB-GAP/TBC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="252"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="373"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="474"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="573"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="666"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="866"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="926"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="972"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="100"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="9"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="263"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="283"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="373"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="407"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="475"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="503"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="666"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="866"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="931"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1014"/>
            <end position="1037"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1409" mass="160676" checksum="7976F33CC7434C38" modified="2022-05-25" version="1">MASTTAATSGVDNGSSHSNSNSSYNNDSIKINLETLKSKIIATNSLVNKYKEIQNQLALTIEENNRLKQQLNNSSNGKSTTTTGTATTAAAAGGDYKVLYVNLKKENVNLKNQLEKEINNSLPLRNTILSYEAEISELKSTLQKKESQLSVDSLNQSQLKAKMVDYQMMQENLEKANKNVKKLKEFRVKYLDTKKQNERLSLEIEGLLKTRNALLSGKMTADYSTLAGGGAKCANCLEKAEVQDLNDELFNSLGQEDKNSSSRNKRNTKSNKKSSNNDDIEDFSNSNNSNNSSNESDSNKNDNSNGNINNNGISDFTDDDEDKYAEHPIHSPSPPREDEIELDKQLTSSIDNDNKQDLDLAAIEEMVEIALQPPIQTTQPPLIQTTLPPSLNKPRPTTISPPLFEKTPTPTLAKTTTPPPIVVQQNDNNNKIVNNSNNSNNDSSAKEFEWDLTSPPSSPSLKENVEMDLEEDIDNMNNESNDNNDSLEDEMEFVPPQPIAQNSEKERDIDIDISDKEHIENQRLESERLEKDRLEKDRLEKEKLDKERLEKEKLERERLEKERLEKERLEKERVEKERLERERLEEKDWKEKERERSERERKRKEEEEKKRIEIEKLEKERLEKERIEKIEAEKREKERLQKEKMEKERLEQERIERERLEKIQAEKKEKERVEREAKEQVEGLEKERLEKERLKKEKHEREQMEKEESLEKEKSEREKREKERRERAERLEKSKQDRKPQNYLKSTLLDSATTATSTLSSSIASTSTPTIIATAAEASSTHISESASNSSSNLLSSNLGATENVVIDTKDNTQNKKPLIRPKKSTIVKSTITTLAAHTSVLAAPETATTTAAVITTPPAVVVTPIPHPSPPTSPLPPSPEQSSDLSTLSFLAQTPDHNHISNDMFFLESPLHSPLSPGIFFETNKTPDHNNSQDSINNSSIDEPINKKPTSTPIITTPIITTPIITTPIAITTKPTTTENNSLKRKSPPKDLFGISNNDLNLENPIKKSKIPNNNNNNNNNNNNNNNNNNNNNNNNNNNSFVNIILKLNELNVNSIQECFESIIEWISLDENKNTEKLCFGILSEFLLFFESNHLFDIKHYFPIICLICNISKNNLKFQELIFKHLISTIIYKQIFSMKNNSIATVSEIYSIHFCQILSTICHLLKNLSPVKILLYNLFQCSNKLVNIDYIKAIHSSVPYLFFIKDDLFMKTIQVIYTNNNTIILNDKKHKKFLQSIGWNKITFSLEQYSVSLVKQVEILIEKNELDSLYSVLKCLELSCQHLGWVWTYNRIIATVFWEHLGSFDSNLKTQLIESIGIMSSTIKYYTDNTNNSDDDDEQKQSTISIVSEGLMFILNDDSSPFELKRTTALALLKICKNPTDLQPLKDWLLSLDQTSKNLLSNSILGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V691</accession>
    <name>A0A8J4V691_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histone deacetylase</fullName>
            <ecNumber evidence="3">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_006107</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone deacetylase family. HD type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000267">
        <property type="protein sequence ID" value="KAF2072584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V691"/>
    <dbReference type="OrthoDB" id="424012at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11599">
        <property type="entry name" value="HDAC_classII_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF5">
        <property type="entry name" value="HISTONE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="12">
        <location>
            <begin position="709"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="105"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="774"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="548"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="239"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="265"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="281"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="304"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="775"/>
            <end position="793"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2072584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1050" mass="116648" checksum="B7B22991DA90512E" modified="2022-05-25" version="1">MMSTLHQIAEASRPGGEKPPCCIGAPRGHCTVHCPRIEDVLYSQRKGKTNKGAQRWRCKACGTKWTSKGIIQPPVVPDTIVNSIGFGNGPEEMSMDMRGVTRPYKKTKTDSIIPTSPFPSMYGNSNTSPNQSPIPSPPSPLSSFFSSRNGQLGISNNYVPILPTSTPSPSNSNGSKSLITTKPVLVGVTSPSNGNNLPSKVPVSSNSFNQNQNQQPPQQQPYLQQQQPPQHHQQHHHQQQLQQNIINPINRPSLSSRLIQPKPSSPGQQAPLSPNSTRLYQSSSQQPPIMPPSKSSSNGLYTRSSFNSSSSNNVTRSISPSKVLLFNNRSPSPIDQNNSNNNNNYVSLRHQDIVNPYSNSNNINNENQQHIYTPSSNIVECLKKLLHSVSVFSNEYNVEHRLKLSNLLDLVSMPSMESQFSSSICNSMLGSIDSHIFNIFKHYQSLYQSISRHHSMMEDNFVPLTDVYFDDNEINRFLLFSEHHKYIEDNFELVLQELQNFKESLHSKKENIEMEIIDPSSVLTFSTSSNSGNLEQTKLETSKLLDSLAPLESTLNSMESNLKQVQKEINIAHKVFSFVELIYSVHINYHMAIIEKHQKLMVNYMLDVIQQNLFNCDVLSSAYNKLKISLASGNFSQMSGNSLSSSKRSRDDEMNLIDTSNPFIHKLNDLLTKYETIKTTSQQNQAPKERKVLAIYHSLCLDHSVPDDHPESPKRLGAVIRAIAEFSRQSDRIVIKNDPEEISDKWITTVHSPEYLRQLEELTDKMDSTEIRPLNVNNDGASTGINQFNSNSSRDSDCEDGDTFISKLSLKAARRAAGATITAIDQVMKGLVTSAFVAARPPGHHAGRDGLTSGTTSQGFCLLNHVCIGAKYAQLKYNVEKIAIVDFDVHHGNGSEEILANDNGFYFLSIHMFEEGFYPGSGGSGSMGVVNHNDYSENGGESGGNIVNIPLDPKSSANSFLKAFSIIIDKLNEYQPELLMISCGFDAHIDDHLASLCLSEDDYVGITRQLRRVADRWCKGRLVSILEGGYNINALKQCTIAHLSALTQDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6S9</accession>
    <name>A0A8J4V6S9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Right handed beta helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_002549</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000071">
        <property type="protein sequence ID" value="KAF2076142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6S9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012332">
        <property type="entry name" value="Autotransporter_pectin_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31318">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31318:SF2">
        <property type="entry name" value="PECTIN LYASE-LIKE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035196099" description="Right handed beta helix domain-containing protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="484"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Right handed beta helix" evidence="4">
        <location>
            <begin position="189"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="451"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="462"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2076142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="518" mass="55644" checksum="B5E5D6B14D629919" modified="2022-05-25" version="1">MKTIIYFTILLLVALICGTSSEVTTTTLIVDPASTSTSVQCGLSAADACPRIIEAYYSFLNSTVAKTNPADSQFPPLVIELVPGDYSKNTYTGNINFYGFDVTIQPYQNQKVVYSGVNTGNSATMFSFSQSGKVLPSLVNIYNITFDKLNCALMNGQMYTPLTVNFENVTISNSQYTSSQLIFFTSYSTNQTVVTFTNTAFDGCSGGLILVQGVYLGFYRSAITNTNGGQPISAYSYATLNITDSLFFKNNVPSANAIIFLNSGNLHLVGTTIQDNNGVAIWGTESVTVTIQTSYILRNTGSNIGAIRVDQSSSLDIQNSTINQNSGNYGGAIYSSASPVTMANCIVYNNLASQGMAIYISGSDLSVTNTSIMYSNDGSSQYTLQNMIYAYNAQVTFTNSSLNLGSQGVVNYFTLIDCTQSTIISYSTNVTTMGHDAMSCSSCTTTNGAENTQDQQVSCPPKSDSSSNGHKNSSSEKKPKKINYAAIFAPIGAVAGVAFIIVIVVIVKRRNRRNYHSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7N1</accession>
    <name>A0A8J4V7N1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004571</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000164">
        <property type="protein sequence ID" value="KAF2074129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7N1"/>
    <dbReference type="OrthoDB" id="16604at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="328"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="397"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="420"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="498"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="538"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="577"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="613" mass="72067" checksum="251837F225926BB4" modified="2022-05-25" version="1">MVKLFSLRSFIVYFNFLVIPVILCCAWLLLPTHTYVAFSGTSMAYFVFVFFTWISFYDLNLPGVSKIQILVFFALFIGLSIGWLHIVIDLDKESNRLAYSFIDAIVFVFCAICYLIHIIVYKMKKILVFRDEPILDADGRRRRKQRSRPWTKRISRIFCYYRVIDMDDADTIRQFTFGAATLPKKGKNSSLTSSVDPNNNSNDLTSSQSSINIDGPKRDLTSSIEIADDITHNEAGDGLQMSTFSSNQPIVHLDNQSNVNNISIVAYDGNNNNNNGNGNEYLKNSDFKQNFTQSEGKKKPDYKVRTQTMVIENPIDLMPKYEISYRNYFSVVFYQLFVIGTWFWLHSFTTLIRENELFSTKIWALISICIFQASRVVLMGISNALNRLLKPKELKYFTLFQFPLMFFLYYRSLFLNTNDWSIVVLVSFVIFFIDVVYYPLHMTKRFWYFRHQVLLPYVDKKRTTSRIFAFVRILLAEENPSYDRHVMNLSIEYYYDKMAEYISIITMVAFLSIIRALKWKREYFKYIAQIEDNTFYQLLYRYLYLLGFEFSYDLAVRFASRRWLKIDISNRGRNETISHYGTRFIFALFVLYDLLDVYNVQLFPPSATSTSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7Y5</accession>
    <name>A0A8J4V7Y5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ras guanine nucleotide exchange factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_004241</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000146">
        <property type="protein sequence ID" value="KAF2074459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10254377at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF369">
        <property type="entry name" value="RAS GUANINE NUCLEOTIDE EXCHANGE FACTOR K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="6">
        <location>
            <begin position="838"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="5">
        <location>
            <begin position="1060"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="115"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="245"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="707"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="779"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="970"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="67"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="89"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="128"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="163"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="252"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="342"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="707"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="972"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="992"/>
            <end position="1005"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2074459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1300" mass="145986" checksum="0F20C05711EDA911" modified="2022-05-25" version="1">MVSTDYIEDEVVDNNNSTNHNSNIDDVVNSPTRSTPPSKKPPPVPLRKASSEKFSVKLFKTKKSASDLKLSSSSSSPTSSPSQPCNINSPSSVSSFSLSSIPEQNKDGCAIAASSASNHHHHNHHTSKSSPVQSSKSFSIPKLLFSPTFKKNSNNNGNHHHNGNGASTPSPISTPASNQQAQQQQQHYDEDSSNSISDGGLTPESDCKLSEIASRTPPTPRLSHESKEIPSIINLNILYPNNNNNNSPRSPVLQSTPLSSISTGDLSNNTINNNYKPELLSISEPNISTPSKHNNKNLYKEKVLSFSPCSTIEEALSIIIPNLMTFNGLKNARQTSNNEWETVVDNSDQSTSQSHIGSESDTNDIHNSFALFKWTEPNVPLHKEKTMLELGLNDRESLILKTVVLNIVFVKILIPHFSEGQLPAQATLRLTQFTSIRSIIRKLYLKFHNNIDISQHGIFLIDPTSRQEVLLDEDELFSTFNINANSQLLFRTLNRDTENEIISLKSLKLKILVSTSFSRYNYLTLNFDPQCTVSKAIKITGLRTGLLDSLNKCGFYLTPSEDDDDGFWMDEDYTLDSYRLKSLTLLEFKERCRKYTVIIKPLDRKCTFKFDQFVKVSTLFNIIINNEMISNAKDYHLVLKNGPTLENDRFLWSYDIKVEIEFRELPNKLLVFGQNGEKNLVYVDFNDPVQDICERLSTNFGYLVGNTGNNESNQDSDSFNNPDSPVTTRERSFTFRKLGHLVNNIDVRKSLKSQGVQPNDALVLEVIEKHHKEQQEYLSNQNGADISDSGSTTPPTKNKSTLSIKNDGTSLNEERQINIWEEPSDSDENIIYGGTGQQTQSIEAGTLNKLIIHLTSKNTHDLMFMKTLLMTYSSYTTAATLLKKLFERFQVPEGIIDEKEKLSIQLRVGNVIKYWVEHHYEDFCYESTKLMVDFVDTHMMIAFPILGNQIRGCILKRTCGIKSILSRTRSNGTLTSPRGSSLLGSSGGSGSGSMNNSPTSPTQFGLSSSFSAIQAPSSAMTSPSSSSLSLSQSFISSPSRKIPESKLKGLINPRSLFDFDDEEIARQLTLFEFNLYSSIKPTEFLNQAWNKPNIASRKSPTILQMISRFNNVGLWVVSLILEPERVKTRAKRMERIIRIAEKLRELKNYNTLMSFIGGLNNSAILRLKYTRGLVAKRYLDSLDSLEKEMSCEGAYKNYRDLLHNTDPPCVPYIGVYLTDLTFTEEGNPNIIGNNLINFAKYTLLYKVISEVQQYQWSEYNLKVVPIIQTFIKDLTPQNASDLYKISLQKEPKGAQKSEIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VAF7</accession>
    <name>A0A8J4VAF7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001593</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000039">
        <property type="protein sequence ID" value="KAF2077084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VAF7"/>
    <dbReference type="OrthoDB" id="10290201at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="556" mass="63374" checksum="FF8DC636F9B81CBB" modified="2022-05-25" version="1">MSFYLIWRDKYLRGLIKNHVLKDLTIKANLGYLAQNQHDLSLLDLNNFNIFINFDTTTIDSFRQYMKFPYRYLINQLDLSFLVTHHFPQDQILPETIKKLVINFPNDNDYVLDFDLEHTIPKSVTALELFNYRGSLAKQDLPSNLKSLVITNLKGQIERDILPESLTFLDLNHYQHSFLPNSLPSNLTTLNLSPDYALPIEQGVLPLSIKSMNLINNIQVLSNINVPVDKIDKVLLKIENHQELELAKNLKWLTKLEILNELKVDLTENLLPESIKHLTLREYSGPLFVGSLPANLKTLIAPQFNHLLEPNVLPRGLKELWIRSYKKPFNYNVLPESLTLLYTGEYNVSQETNASLHNIKHLTVVENNQSCPFPPSLNTLYLIIHNPTINITKDQIPTSVTSLSLYYQSIGVVELPQNSIKTLILLNPRAPQLGNNLVIPPSVTNLQIRSPFKANFIPNTIRNLKMHYFSTLEAGFIPEGVVNVTISGLTMPGLQINVLPKSAKTVEFLNSKYFEFGSIPENISWLKSDSSIIKSSSFPKTLNTISICGIFYNNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VAH5</accession>
    <name>A0A8J4VAH5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001529</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000037">
        <property type="protein sequence ID" value="KAF2077144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VAH5"/>
    <dbReference type="OrthoDB" id="410267at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17353">
        <property type="entry name" value="MFS_OFA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF317">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="345"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="370"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="406"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="449"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="3">
        <location>
            <begin position="37"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="556" mass="61756" checksum="B08BC32913A5EB44" modified="2022-05-25" version="1">MAVEKKNLFTRVVEYHYNRPKPIVQLSDKQKEREKYIFGVLPFNRWLLFPSAVLVQFCIGSFYAWSIYNKPIDKAIYGNENQGMAPITFYITVGVFGISAALMGPWIERHGPRLGVFIGAILFTLGHYLTAVGIHFQSIWVVYIGYGVFGGFGIAITYISPVSTLQKWFPNHRGLSGGFAVCGFGAGSVAFGQIPLPIIKSVGLPLNFVIIGSMFFVILTLQCFILRVAPDSMSSSTTSGNISSESVDQEMGSPVIEEENYIPSVNDKTITQSLLSKEFRLLYIVFLANSMFGLIALSRLSNMIQDIFGKSKETAAMVVTVNGIFNLTGRLGFALLSDKIGRKPCYIITLSAQAIIVGILPSLVENRSSSAFIALIWLLTSAYGGGFGIIPGFLTDLFGPKKVSTCHGIILTAWSVSGIFGGVIFTEIMNALKHSGYTVHDPIIYTTNFHWIICIILIGWICIWFVQTNERDILTPPIKGQTFKIRLTSSILLRISTTKGFERVSQEQQDLEWKQYCEDYKQKKLQELNDNTNNDTEQQIHPPTINDNASDLMAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJM3</accession>
    <name>A0A8J4PJM3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MYND-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_009832</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000823">
        <property type="protein sequence ID" value="KAF2068845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJM3"/>
    <dbReference type="OrthoDB" id="443682at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007320">
        <property type="entry name" value="PDCD2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12298">
        <property type="entry name" value="PCDC2 PROGRAMMED CELL DEATH PROTEIN 2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12298:SF4">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04194">
        <property type="entry name" value="PDCD2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="6">
        <location>
            <begin position="128"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="200"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="217"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2068845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="409" mass="47594" checksum="F58BB2DFC0134039" modified="2022-05-25" version="1">MKVSIGYAEEPYDQNELTSDFFPNKVGGKPAWLDQSSIPKREELKCSKCSKQLSFLLQINAPIDGKDHCYHRTIHLFCCLSNECKNFVAIRTQLPQVNEIYPVDADERKYEQDYKIEKQYYEKRQKQCEYCGLFAAAHCAGCNKVSYCCKEHQTIDWDLGHREQCKILKQQEDGAQVELPAKRKSEFHFKEYEILNEDSDVVAKPTNTVDPNYHNENDDDDVEDDDDEEDQDSEANQKILEELMLKEDTGAPAAGSSNEVSTTSDATEQLNEFVEESGLDGELIDDNKFMNFKDQEFLYFKRVIAQDKNQILRYSRDNEYPILWVSEENKVTDEEIGKCPCCNGDRRFEFQVLPQLLYFILDESTSTEVNNNELDFGILNIYTCENSCEYKSNQLNYVKEFIFKQDFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJQ2</accession>
    <name>A0A8J4PJQ2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_009889</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the THOC3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000844">
        <property type="protein sequence ID" value="KAF2068787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJQ2"/>
    <dbReference type="OrthoDB" id="340259at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040132">
        <property type="entry name" value="Tex1/THOC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22839:SF0">
        <property type="entry name" value="THO COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22839">
        <property type="entry name" value="THO COMPLEX SUBUNIT 3 THO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25174">
        <property type="entry name" value="Beta-prop_THOC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="144"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="187"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="316"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="68"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="76"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00046343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2068787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="441" mass="49215" checksum="CDF0EE9894FDEDC2" modified="2022-05-25" version="1">MGSRLNPQSNIYLPMGNPNMPRGYFMPNMVNPNMQQHHMYNIYHQSFQQQQQQQHQQQQHISSQQILNPSLSVPPSSTLASNATTNSSSNATASSSSSSSTSSSSTSTTTSSANVSKTNTPYTLFDKNLELATRYFTECTTKDYAGHKKKVNSVHWSLDGKKLASGGVDNTVRVWGIDTTKGKELEIKGHTDTVEQLAWSPINSDILATASSDKTIKIWDTRSGKCTTTINTGGENINLAWYIDGQYMASGTRDDQVSLIDLQIPKIVKNYKYNEEINEISWDKKGELFFMTTGSGTIEIYKYQSKTPDLKSIKTLYAHPTNIYCMDFDPSGKYFAVGSADSLISLWDYEEMFCIRTFGKIGFPARTISFSFDSQFIAYSSEESFVEIAHVESGQSIFQINCDLGLNCVSWHPTQPLLAMAFDEKETTKDSGTIRVFGFHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJS9</accession>
    <name>A0A8J4PJS9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Actin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_010281</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001014">
        <property type="protein sequence ID" value="KAF2068394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJS9"/>
    <dbReference type="OrthoDB" id="5132116at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000148">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2068394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="53" mass="5463" checksum="557B68BBFFD256B2" modified="2022-05-25" version="1" fragment="single">MEGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJU7</accession>
    <name>A0A8J4PJU7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009627</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000760">
        <property type="protein sequence ID" value="KAF2069055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJU7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="144" mass="15574" checksum="2C78216A77D24836" modified="2022-05-25" version="1">MEFDCSVIVDDRTSFHINNGQGASCTGVLPGSVSNVSIIYDYENRDNRSDLVETMIKQLGSSVVSLDLPNQYKFTRKDLSWLGRITCYAVEPMTLTAGLLGPQINYIHIANLVGPLAAGVLPSALETLVDYNKPLAANVLPASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PK06</accession>
    <name>A0A8J4PK06_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_009574</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000747">
        <property type="protein sequence ID" value="KAF2069102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PK06"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11042">
        <property type="entry name" value="CYP51-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050529">
        <property type="entry name" value="CYP450_sterol_14alpha_dmase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304:SF2">
        <property type="entry name" value="24-HYDROXYCHOLESTEROL 7-ALPHA-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035314866" description="Cytochrome P450 family protein" evidence="7">
        <location>
            <begin position="25"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="413"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2069102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="465" mass="52435" checksum="9D12715E764DBEE0" modified="2022-05-25" version="1">MIGVIVIVIAVLAFLTLFKKQPAGLPPVVGTIPFIGCFPQFAKDSLGLVKRSYEKYGDCFTLSLMGFKMTYLVGPEAQAVFFRGTDEELSPKEAYKFVIPVFGKGVVYDSETEVMYEQLRFVKNGLVLSQLKKAVGIIAEEAEKYFEEKWGDAGEIDLLNEMNKLTILTASRCLMGKSINRALGKADQLADLYHELEEGLNPISFFFPNLPLPSFKRRDAAREKVAAIFHSIIQERRKSTDDTVDDVLYTLMNSKYKDGSELLDEQIVGIMIGLLFAGQHTSSITLTYTIFYLLNNAQYLDPVMSDIKEFVQEESNGEINFDNLKKMTRLETVIREVLRLHPPLIFLMRKAMEPMQYKDITIPAGHLVAVSPQVGMRLESVYKNANEFRPERFDQEEKTPFSFIAFGGGKHGCPGENFGILQIKTIWTIISQKYNLELGPVPKTDFTSLVAGPKGPCMVKYTRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PK23</accession>
    <name>A0A8J4PK23_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">IPT/TIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010569</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001204">
        <property type="protein sequence ID" value="KAF2068105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PK23"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035144818" description="IPT/TIG domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="544" mass="61253" checksum="E0397585A89B6532" modified="2022-05-25" version="1" fragment="single">MFKNLYIFILLLCLFGFSRGWIRPTVLIQPKPGYGDRNRLHGLRIPTTTYFDSPYGGCNDRTPCYCRINGDTRYNGCSFKSLDSITINLNINNEKTFPPVDENKQFKILCRSWDYSNTEDSNIVFYDLISYTSSYQRNLDFYIVGDWEKISDKGNIGLLGSKEGGPNSVYQRLTYVNSTHLTIRITPELNAIRNSLVLYDLNTYTSVCDIGPANDFKPWIDQYYFSGDSEFHVFGAVLTDRSIEIGEYPNNVSCPLIAGPTYTKIGCKPPSYLLSAKSFIFSIYGKSRDPTSTVTYTQTFPHYFSSFKNLQDGKTCELPISFPSISHSQSLTIHGLSAAIKPISYSPIKFEYPPEAKCGTAFIMLNGRRTSNNLDMCPTPNITDIITRPDEFNGNLLTFKGDFLYNSFLYGDGSKKSINFVILYRDGSNSSCIHKMNGLSDWNSQDRTYSISCFISKIYTFKIRTETILGQIGIFDGVAYPPTITRWSKTFDYLVPSKITITGTQFGQGMLAKVADAPCSNPVVNNEGTELVCDFSNNVSDPND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PK76</accession>
    <name>A0A8J4PK76_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009411</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000702">
        <property type="protein sequence ID" value="KAF2069267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PK76"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="97" mass="10766" checksum="64D04706BB63448F" modified="2022-05-25" version="1" fragment="single">MINIDSNISHKSGQIQDSETKFKQFAKELNTTFISTIKTTSTYNDSAFHTVNNSVTFVKKSTDLYSPKNIKNKTIMFMGPSGAGKSTLMNYICGVPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKJ1</accession>
    <name>A0A8J4PKJ1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010136</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000940">
        <property type="protein sequence ID" value="KAF2068538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKJ1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1147" mass="131836" checksum="DEDD5FE66AC2C364" modified="2022-05-25" version="1" fragment="single">MRTAVFVTKALVPQPCTSHTIDNNRVVLVVGPDIDKFSWADVVLVNQQKTIDTLVIQFSRQYRPNQDDVIIKKVILNLPPEITTIKMILESGIVLYQRIKEIISVGSPFINRNVLCDNVDNNRFVHWKMSDEYNSFYNITIQDDNTEYVLEYLKTIQSYKKKSNNNNHSIDNNSNNNNIIRKDLVFNVYNGNSSNILPIVDMLRNHIQSMKIFKSEEIKEVKEKQKEYKESEKDQEESGLVEHFSWRKSKLPKAGIIPNGIKKITFFNCQDPIPVGVIPDSVVYIKFKRLSICLSQISFPPNLKFLSIDKFNQFLIGQVLPPTLSHLRVTLYDNTYAHNYLSFKGTLPRNLSHLKIKKEFSHIYFDPPLLVPSNIKNLEIQENGNSDIQFVDPDEYHCNPITKDSLECLHSLKSITLNGDFATPNFFPNDLQSLDISVLPTFDINLIPTTLTSLICGSQDQIPSHVKLNYLKYRITGKKTMVFKVNDRSITRDNIPLHLDRNTNQFVPKPTIPIDELEWNEDSEDSKDEFKNIIPDSIIRKVKSNLPVYIPSSVEIYESNCLPINYPNSLETIILTGIGTKDIHFDAIPPTVKNLLFETDNEFLNKYSNENGLLKDKNSLYQIDYNYKNSIKESINSNNNFIGNDLYFYIWRNKYLHTKINQLLQPKLIEFTIEREGTSQNPRYHFKNQDNQQVHNLKFKSEDIASIPNGVSYYSVRNRAYGAKLLESLPSSVTKLRLSASNIDSITLPNIKHFIVDKSLNFKEFLMPSLETLEIKRPLDLRDLLKKSTVKRVILKPGFFENGQKSKGFYREILKYLNENNLLTDGRYEIYKAGGPIPPSTIVLVWNKNQMIPHNIIPHGVKRIIFGRTFNQAILFDTIPSSVTEINFGQKFNQLSNIFLPQSVKYISLYFKSCSPLVHTNYKQVTWPPNLIHLKIKDYEGSLYFNPIAFLPKSIKYLWINQHRFKQDMDNKTSFFKIKSIYSNGNINNTAEKMISSINNYIDDKLKDAPMVDVFTSTSSRDSSKPISFPNIKSITFKNNFNQAIPEGLISSSCPAISKLIFPKASKFNQQLFVVPPTVQHLELGSEFNQELKSFMIPASLTKLYLSKSFNQPIPIGILPNGLKVLDLGSSFDQFISIGTIPDTVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKK8</accession>
    <name>A0A8J4PKK8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">malate dehydrogenase</fullName>
            <ecNumber evidence="4">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_009967</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible oxidation of malate to oxaloacetate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LDH/MDH superfamily. MDH type 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000868">
        <property type="protein sequence ID" value="KAF2068707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKK8"/>
    <dbReference type="OrthoDB" id="4069699at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01338">
        <property type="entry name" value="MDH_chloroplast-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000010">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.110.10:FF:000014">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01517">
        <property type="entry name" value="Malate_dehydrog_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010945">
        <property type="entry name" value="Malate_DH_type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01759">
        <property type="entry name" value="MalateDH-SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003916">
        <property type="entry name" value="PRK05442.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23382">
        <property type="entry name" value="MALATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <keyword evidence="9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="11">
        <location>
            <begin position="31"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="12">
        <location>
            <begin position="183"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="397"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="580"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="667"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="597"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="618"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="682"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2068707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="705" mass="76973" checksum="C5C62D1B7DD733DF" modified="2022-05-25" version="1" fragment="single">MFKGLTKVMNGARHYSTGSGFFATGQGKKPVRVAVTGASGQIGYQLLFRIASGDMLGKDQPIILQCLELPQAMNSLKGVAMELDDCAFPLLKGIVQSDNPNEAFAGADYALLVGARPRSKGMERGDLLKANAEIFSVQGKALNDNANADTLRVLVVGNPANTNALIAARNAPKIDPKRFSAMTRLDHNRGLAQLSSKVGCAVTEIDKFCIWGNHSATQYPDINFGSIKGKKITDLVDAAWTKDTFIPCVQQRGAAIIAARGLSSAASAASAAIDHMRDWTYGTNGEWTSMAIYSEGEYGADKGLYFSYPVIVSPSGKYEIVKDLKLNEFSKERFEKTNKELCEEHANPSKIIFSPKSESIRDVQFNPFQPYQFASAFDNGTVQIWDTRKQTSAVEKITAHQGLVLSLDWHPEEKSVIATGGRDRAIRIWDFTPSQVGGVMGKALHNLSTISSVYKVGRGHKWQLASYSSIVDFQTIDVPTGLLWRNPNTLVSCSKDGYLLINSINDACKPYQHIRTTGISWNVNNDIASINDKINRNQNNESTSLSNQFPSFFASFNVPAIPIQAPISYFKNLGIDEQQQEKKLKRTSSAHLHIDDNSAVNNNGNSKANNRVNDNIEDMSSSSSGNVTKNNDMAAKEPPSTSVYMESKNDQLLSNLLSAEAVTPLAPLPSRAPSIEIEKSKSSSPILYNDNSNNINNSSSIDDQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKQ1</accession>
    <name>A0A8J4PKQ1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MRH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009883</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000841">
        <property type="protein sequence ID" value="KAF2068798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKQ1"/>
    <dbReference type="OrthoDB" id="10372792at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035210467" description="MRH domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="20692" checksum="959A7132B95E41F6" modified="2022-05-25" version="1">MYKTCFKILLLLTVFVTISTCIKIDTPIDIGSGDTQKCIFTDDGGKTIDLTPLQNIEYSYFDQYSLSEYHYGICSPSTHCQDILNTEVEVESCGIFETNGAPNVTYAIGLFAGGTAQVFTEVTNLFYYQNVANNVCPGGKMIMQLELNCNYFYDFVVHAVLQPRTCHTIISIGTIHACEKNSLVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL04</accession>
    <name>A0A8J4PL04_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010195</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000967">
        <property type="protein sequence ID" value="KAF2068479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL04"/>
    <dbReference type="OrthoDB" id="2307943at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="187" mass="22152" checksum="F4C08F5BF0216A6D" modified="2022-05-25" version="1">MILENIKSNVVSYQLLKTWVFMLSFMIFVGLKDYNPRNLVPVGMRPQLEEWSRAKFYQTIVKALKRKGYQRTQYSMRGKYCSESQAMEDAYTLHQEPNMEWLPFCIQKLHLVKLVTLENIYVPFDPIDDDTDSDVDIPDDSDIDDSDKDDDDQIGVIINWVKWQNRFQNLIKSQFQDENSLSHCFFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL57</accession>
    <name>A0A8J4PL57_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_009807</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000814">
        <property type="protein sequence ID" value="KAF2068870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="26679at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035859">
        <property type="term" value="C:Seh1-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049567">
        <property type="entry name" value="WDR59-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46170">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46170:SF1">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="161"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="249"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="LysM" evidence="5">
        <location>
            <begin position="731"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="domain" description="TLDc" evidence="6">
        <location>
            <begin position="1290"/>
            <end position="1450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="387"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="582"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1222"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="10"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="387"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="586"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="609"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="635"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="677"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1226"/>
            <end position="1238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2068870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1450" mass="161187" checksum="BD2D17029570027F" modified="2022-05-25" version="1">MEVFDPPSTPAAAAAPTTPTNNNNSMNNQQQQQQQQQQHNYYHCARVKQYQPAHMINITVNRKNKYKFHTVAADCTGVNVAVSSDKALWLYNINDMIQTNDDDQYSTRVNHKITSTKNISSDLLVWSPNRENSHLLMSTCPTDQTLYLWDLKEGKSLMQYQKSHSRHLTSLSWNKVDSNIVCSASLDSFIKLWDIRNGKKDAMIDSIQLKSNLSQICWNPLSSTMLASSNAGEINIWDIRKFGYPLTSFTAHIGSISSIDWSLEEKNKILTCGSDKLVKVWDISPPNKNLKVYQTGSSCIKAKFVPNSKSVASISQKGYNEVCFWSGDNRSETSLSLEGHKNQLISFDFTIINNENTGQDSYHVVSIGKDEYLNVWKLNDDMFDKLNNNGNSSGSNNQLSNSNNSSSDTSELDSSGDNNVSEIIPNSPIIISSNNNNNSNNSNNNNDTPRLFVPVDLQQELGKVKLDRFKYLTLEKVSYSERICMVSVDSRVFVVIILFPSLYPSAPPSFDIVFGDSSSEITAFQVKLKKGLDKLSHELLKTKKPFLLQILQYLSDYYSQNNSAEFLRTLTRVDDTLVQKKIPKSVANQPSKRHSITPTSPNVLNMLINNSGSSGGSSNNSPTMNGNVAEWDPLTSSAYSSSSSMSTTNSGFSLQSSPSVTSTPTPPPAINIITSPLSGSTTRNRSYSASSNLNQGGEARSRSISFGGGHSIAPLPPNISSISSNQALLFEMYVVKPTDTLAGIALQYSMPRDVLVQANRLHSDKLMPGTILWVYKKKNILDQLTSPSLQPLDGSSSVGSVNMSSSFNPSSLSNLTNTTPSSSLTDFATLSISESNDNLSNIDSSMGSLPLSPTSSWIPPTSPTPYGSLNPSFIENIQTMNQIKANSLLQQMRAKVKTTSPTVNINSLFKPLKEEVEMAFQKSQDGAAHAHMSASLSSANLTGAPTTTTTATNSYTASWNSLNTNRKFTQIPKESIVKENLVCFTKDKKVYGTLTLTPYQMFFQSIHSSTNSKPTVLFADYSQIVSCKYFPNKTEWIAHLSKDWNKEIKYVDQKNSKVSKEDQDRYNKFIQSEISKLNEMEDESTTIKDFDPTYNDQDKKVLVFPCIYVLLQMDKFIQTLFFRGFQVDSVHHCFSYLKKLIVDSKLASPPTTSPAMTGANSAGNSIGIIPTYQPSSLDVVKEDSSNDIAQVSTSIPIQIPRSIGLGNSSLFGDSIVLGDDYKRTSDSSSSAKPTATSTQPINTSTTSNMFRIPSYNNIKSLSASSGSFDEYVHSPKLLKDQIRYHHQQEIIMNPEIFKKLRHYLPIRTQGSDIELVYNSTNDGVSFTTFYRRMSQVDQSILLIKDNKGYIFGAFLSEQVKPKKDVFYGNGETFLFKLYPEFAVYKWTKENDLFIYSSHDYISIGGGSMFGLWMNNDFLNGYSGPCETFNNSTLSFENDFRPIIVECWGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLF3</accession>
    <name>A0A8J4PLF3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009535</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000734">
        <property type="protein sequence ID" value="KAF2069143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLF3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="6"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="103" mass="12253" checksum="AA45C6482A866F2A" modified="2022-05-25" version="1">MIQIFLSNMSNSREDLEKDNARLQKENWELKNYFKVGCSNTDHNSFYVQLETNNNESDRLIQENKAIKQQLEKLSEENDSYYNKMDMLLNKIESLANITIVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLF5</accession>
    <name>A0A8J4PLF5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sphingolipid delta4-desaturase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_009646</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000766">
        <property type="protein sequence ID" value="KAF2069035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLF5"/>
    <dbReference type="OrthoDB" id="200948at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042284">
        <property type="term" value="F:sphingolipid delta-4 desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013866">
        <property type="entry name" value="Sphingolipid_d4-desaturase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12879">
        <property type="entry name" value="SPHINGOLIPID DELTA 4 DESATURASE/C-4 HYDROXYLASE PROTEIN DES2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12879:SF8">
        <property type="entry name" value="SPHINGOLIPID DELTA(4)-DESATURASE DES1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01269">
        <property type="entry name" value="Lipid_DES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="236"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Sphingolipid delta4-desaturase N-terminal" evidence="3">
        <location>
            <begin position="32"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2069035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="352" mass="40659" checksum="5E89AEF4DCE10C76" modified="2022-05-25" version="1">MIPIDEKPTVRKSKTGLPATIGHGPMKQEEYNPKDEYIRVESVQQHWIRRKIISEKYPEVLNFPATYLPSAFYVLGLVSSLVLTAKLLQNSPVWLILVVAYVFGATASHALWVLIHELTHDLVFEGSFFNSVFLLIADLPHIVPAGVSFRHFHRLHHSFLNETYTDPDVPVPFENQFFGHTVVGKAVWFCFYSIVIALRSVFYPLKNQSPFSMWLVANYICNITFTYLVYQWVGVYGMTFLVLSSLMSVGFLLHPLGARWIAEHFAVTQSEQETYSTYGIINTLGFNIGYHNEHHDFVRVPYVYLPKITSIAAEYYTKGLKYHTSYWRVLYEFLNQDGFTFASRVVRDPKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNT1</accession>
    <name>A0A8J4PNT1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 12 13">Diphosphomevalonate decarboxylase</fullName>
            <ecNumber evidence="2 12 13">4.1.1.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_008301</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the ATP dependent decarboxylation of (R)-5-diphosphomevalonate to form isopentenyl diphosphate (IPP). Functions in the mevalonate (MVA) pathway leading to isopentenyl diphosphate (IPP), a key precursor for the biosynthesis of isoprenoids and sterol synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>(R)-5-diphosphomevalonate + ATP = isopentenyl diphosphate + ADP + phosphate + CO2</text>
            <dbReference type="Rhea" id="RHEA:23732"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57557"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.1.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="13">Steroid biosynthesis; cholesterol biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 12 13">Belongs to the diphosphomevalonate decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.33" evidence="2 12 13"/>
    <dbReference type="EMBL" id="AJWJ01000500">
        <property type="protein sequence ID" value="KAF2070372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNT1"/>
    <dbReference type="OrthoDB" id="10253702at2759"/>
    <dbReference type="UniPathway" id="UPA00063"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004163">
        <property type="term" value="F:diphosphomevalonate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006695">
        <property type="term" value="P:cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000018">
        <property type="entry name" value="Diphosphomevalonate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.890:FF:000005">
        <property type="entry name" value="Diphosphomevalonate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005935">
        <property type="entry name" value="Mev_decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029765">
        <property type="entry name" value="Mev_diP_decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053859">
        <property type="entry name" value="MVD-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041431">
        <property type="entry name" value="Mvd1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01240">
        <property type="entry name" value="mevDPdecarb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10977">
        <property type="entry name" value="DIPHOSPHOMEVALONATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10977:SF3">
        <property type="entry name" value="DIPHOSPHOMEVALONATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18376">
        <property type="entry name" value="MDD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22700">
        <property type="entry name" value="MVD-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015950">
        <property type="entry name" value="Mev_P_decrbx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0152">Cholesterol biosynthesis</keyword>
    <keyword evidence="13" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="3 13" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 12 13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 12 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="10 13" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="7 13" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="9 13" id="KW-1207">Sterol metabolism</keyword>
    <feature type="domain" description="Diphosphomevalonate decarboxylase-like N-terminal" evidence="15">
        <location>
            <begin position="10"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Mvd1 C-terminal" evidence="14">
        <location>
            <begin position="183"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR015950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF18376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF22700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2070372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="388" mass="42816" checksum="39B3EBB7E705B958" modified="2022-05-25" version="1">MVLASVTCSAPVNIAVIKYWGKRDEKLILPLNSSLSGTLHQDDLKTTTTIVASESFTEDALWLNGKKEDINAVRYQNVLNAIRSRATKLTDKKHFVHIASINNFPTAAGLASSASGYCCLVFTLAQLYGVDGDISAIARMGSGSACRSMFGGFVKWEMGQKEDGSDSVAIQVQPEQHWPEMNIIVLVVNDKKKETSSTDGMQKSAATSVMMKERCAVTVPQRMKDIEEAIKNKDFETFGDITMKDSDDFHEVCATTTPPIYYLNDTSRYIINLINRYNKASGSIKCAYTFDAGPNACVYLPKDNVVEVLSLFLKHFPGQDISTYYRGPKENIAAIESYQPPKNLQSLFSPETTFDSLKYILHTRVGPGPIVLNESDSLIDNTTGLPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNY0</accession>
    <name>A0A8J4PNY0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007930</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000450">
        <property type="protein sequence ID" value="KAF2070749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNY0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20916">
        <property type="entry name" value="CYSTEINE AND GLYCINE-RICH PROTEIN 2 BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20916:SF26">
        <property type="entry name" value="CYSTEINE-RICH PROTEIN 2-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="522" mass="61510" checksum="C89C6D79BC5E45F7" modified="2022-05-25" version="1">MNKFNTRNSNNNNSSSRRSGDSFLVIFRNVVLRNQIIGYIVPNKEWATTFKNGQYPIHLYDMRFRIDNNNNYVIKDQLLRFSRLINNNIDYSTLDSSRSDGDGDNVKKSRDHTFYQDWIDFPQFYSNINYSLRDIVDKELQDCNNTIARNGSNNKRNHPSCYEFYGSRELGIFNELDEPDQILSDFDDGSNGFRDFSIKSNRLELSEIRPMFVNAQGEVQNLQQLQLKIMQAYRLLAASVSHRKEHYAHNLLVLLSVYPNLQLFDYDNRVGLFENIFQIVFLFNPENYVNGNNINNKNNSNNNNNNNNKNSNYSLLKFHFLFDSLREGKLAFVRLVLDHLNEMESRYIQQNIQRLLLEVLYDSHYIYLKPILYLFSRYSEFIQESILDSIVSIALMNNNHCLLDILLHKTMVRLCKPDSKEHDQKILARINQCIELSNYKDRTSYFDNQLEQHQHQSYFLPPPLHPFIRQEMKELCVPSQPLPVSTAALTKAPQQEGVFRDYERNINENCLSDHEILIYINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP88</accession>
    <name>A0A8J4PP88_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007440</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000397">
        <property type="protein sequence ID" value="KAF2071238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP88"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="574" mass="66546" checksum="FABAB78CD8452D61" modified="2022-05-25" version="1">MALTDKILIKKILDESISYFRDTHRIKTITVDEIAPTILNLLLISKDIKREVIHLNFPILSISSAKEFQYVSRCHIKFKLSLQFSLIKTLFNPSIQQQLYQKFKNFFPKTTTLSTDEAFIKIQENIDCIDQVSDLMFLNDLCDRLKKDNEETLLSKVTSMKISVEHVAFLEIVAKHFGNQLRKLDSCSTQVQLNYDYLNQFPKLEECTFNIGFVVNLFRYLKHRPNNLIHTVSLNSFESLVHRNIAQNISDNPTLTRLSLRGRQTTREELFKILNTCTNIKQLAIYDPNHMEIQTDDSVNTIEITNNTLEKLKLYKPGTFIYNWIGQSILSTLCVDHFTLDMVRTHVNLKKLTLKIDPHSFLDKYILEIWTSHDRLESLEKLKIEFNTIAPSMDNNFYWNVFIKGLQTNYCKLKSLSLIKTNIPLALIGDLMDILPASLTKLDIRIFKILKIKSRDNSIRAATSDPQEIPFYLSIISILKEKLQQLQHLNLKLDMGLDLLSECILVVLECHPFLKSFTFTSFIGGSFNEKQLTRFENALSKSMVQSLNIDPLLWNSSNRNSLSKYLHSKGILMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPB4</accession>
    <name>A0A8J4PPB4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Histone deacetylase</fullName>
            <ecNumber evidence="2 10">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_008140</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the histone deacetylase family. HD Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="2 10"/>
    <dbReference type="EMBL" id="AJWJ01000477">
        <property type="protein sequence ID" value="KAF2070550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPB4"/>
    <dbReference type="OrthoDB" id="1918432at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000001">
        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003084">
        <property type="entry name" value="HDAC_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF36">
        <property type="entry name" value="HISTONE DEACETYLASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037913">
        <property type="entry name" value="His_deacetylse_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01271">
        <property type="entry name" value="HISDACETLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="15">
        <location>
            <begin position="35"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="408"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2070550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="433" mass="49969" checksum="EA15E76F692D3DD4" modified="2022-05-25" version="1">MDNTEFDTILNSTSKTRVCYFFDQQVGSYFYGPYHPMKPHRLCLTNNLVLNYGLHKKMDLYKARRADEEDMLKFHSSDYVDFLQRVTPDSVNEWKDFLKRFHIGEDCPVFPGLWDYCSIYSAGSIEGAMKLNHRMYDIAINWSGGLHHARKDEASGFCYVNDIVLAILELLKYHPRVLYIDIDVHHGDGVQEAFYSTNRVMTVSFHKYDGEFFPGTGNLDDIGIHNGKLYSVNVPLRDGIDDKSYLSIFKPVIQGVMDYYRPSAIVLQCGADSLRFDRLGCFNLTIKGHAECVRFVKSFNVPTMVLGGGGYTVRNVARCWTYETAVCVDTEVSNELPYNDYIQFYSPDFQLIPDHSHIPYKFENNNPKHYLENLKIKILENLRILQWAPAVQIQEVPPDIMSLDIDENLDSKDNMDKRKKKHPDFASLSDQSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPL0</accession>
    <name>A0A8J4PPL0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007909</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000447">
        <property type="protein sequence ID" value="KAF2070777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPL0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035278631" evidence="2">
        <location>
            <begin position="24"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="320"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="653" mass="76212" checksum="A1DDEA2595E4EDB1" modified="2022-05-25" version="1">MMGHTYTPTVFREFFLLIIFIFSKKINDPSTSVTIDPSSGEVAIDGITLSDYTQDHYAETEVIKDDQSGLVIYPEFSAIGVQFARNGHTEVLLNSHHNDVQFMKHFFGDQASTPITVVDNHDVIMLQWIDSLIRSFIIPLPDRKYFPQGYIEAKMTFFRLLVNFLTNLSWENISILKYFINHMVNYNHFLESIELYDRVSLPPYYSYLEPNLKFMMKILASAKLNHLESLENLFWSFKFDSNFLCYFKYLGEIELIEQQINQNQLSLKERQHREKNHVQEILEKTHLGEQQAQQQKYFQDKQKLFEKQKEHQWIIHQLYNNNNNNNNNNNNNNPKTTTTTTSSSSSSSKLIVDELQHKRYLVNNFIKQMQSLEDVYRGNQINNNHQKEYTFPTTNIDTVFDRKGRSTLEKDLSLPVDYYQFRGIFGDEIKNPSNQKNNSKALLGDGFLKLLNFIYQPFVTELVLERILSPNILLYVENHDLYKSVSFVMTVCSRDGSYRPHKFEYRHIPNSNGYYILKVSIEDENKEPKKKKKNPASVTLCFTAYSHDGLPIGEVESPPVIVRTTTNEFDDPSSLLIRNDPQGTPKKVYIAANFFKQGKLNSNHGLFIKFKSNPNVIIKEDEIKFESSIHISFTLPDQVVNLPHDDYIVGYQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPR1</accession>
    <name>A0A8J4PPR1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007379</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000390">
        <property type="protein sequence ID" value="KAF2071304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPR1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="169" mass="19602" checksum="AC0FC64247990AA8" modified="2022-05-25" version="1">MDLITVNSLTDENITIIIDYLLTTKIYGSNTTFLEHSERGMVCNLVPHSNGFHHICLNRFASLIGGVMMGKQCIEALYWRFRYKGQKLEPGFTLSHLDVDKRIIECVQESITNKEFRKYCHHFHWYRQINNEDTIRCPHKEHPCTGPPIDLESSYDQVVHVQTFASSAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPR9</accession>
    <name>A0A8J4PPR9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Terpene synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008113</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000474">
        <property type="protein sequence ID" value="KAF2070566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPR9"/>
    <dbReference type="OrthoDB" id="2861623at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19086">
        <property type="entry name" value="Terpene_syn_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="313" mass="36907" checksum="2551C8521C8CD205" modified="2022-05-25" version="1">MNFEWKLSLVKNQNTKTPSLKCNYPYSFNPYIEEIQCDRKEFLKSINIFPNEVMLNKYLCGRCDVLLAGWAPNANKEEFLKMVLLGDLLIILDDVYIETGKYTEDQIKNIFNSKDSSNPLSKEINDILLFFRQKKSDPFIIDLFKKELANVFVNILRNDSSHKFTGDLDFNQYQQLRFMDFGTKNFFLAPLLLDDYNINKEFYESDLLNQFFSIGSIPISLLNDIYSYKKESQCSDFKNYVKVVAHQNGYDLQLAFTKCTQLYYEYLSKFDDICNQILEKYKGNESVNHLIKTSQLMQATILYFHETSPRYQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPV2</accession>
    <name>A0A8J4PPV2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006957</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000344">
        <property type="protein sequence ID" value="KAF2071733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPV2"/>
    <dbReference type="OrthoDB" id="17475at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="686"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="749"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="23"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="247"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="830" mass="91731" checksum="73023D92D3902995" modified="2022-05-25" version="1">MNKFNKIFRKSVQISPSDLNNLQQQQQQQQQPYGSGLNNSTGNLGSTSGASASKPPHPVTLSSSNSSPNLIHNNSNNNNNNSPRGGGSYINHSASSSSSIAGSVSNSQSNLYGQHQSLPLIDFDVIFGSFVKDGDTVKNYFLVHSVLSRKGPTSTSSGNLLSPNQQQQQPHMKSMLPFYKKNSFACFLVICKRSLLVIDATTYPFSVSKRIPYGDIKEILFDTSEVGFFSIELNSPSSSQQQKNDRSNSNGSNRSNSNGSQQQLQKIDTNNIYYFTTGCEKKEILDQVSLTLDEAMRDNNNLVVKKSDIFGSVDQSYKKDLKQKVNELLDDISLYGFTDQDLWEGNADLKYWVSKIKDNVILNNDERFVIDFILPQLALETIGSLKKNFRVPNNISIREITHFLCSKLQIPDHTLYTLATIKGGELNQDDILADYGVGSFFDNWQLCLVEIGKSRKAGLFNVEVHFPETHEFQGRYHRILELDGYLCASKLMLYIGQQMDIKKAHLYAIKLEILSNSSGMLSVTSSKILEDTDYLTTHGLGSKFLKCRLKLMPKKFPKASKDKQKNMSITLSIIDDIVDNAWGIYNERKAVKSQVLCEAIVNSIIDNVYLECKRASTLTQRIASLSAVGRAAMYKLLTQKEQEDINFFKVDGQKKTANEAKDLINSLTPLTGPSMDLTLEEEKTGLQVRVPPPPPPPILGFKNFKPKKETTSSSSSSHSSSGGGMVRASMAPGASIDMNQILGMRGKLRATTKKEDGDQSTAAATGGEKDKKIIHFTLRNTKDIKPASSLNSSASNAAAAKETNELMLKLQKRHAAVNAIMTDAEDLGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPZ3</accession>
    <name>A0A8J4PPZ3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin-like 1-activating enzyme E1A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_007970</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ubiquitin-activating E1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000455">
        <property type="protein sequence ID" value="KAF2070716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPZ3"/>
    <dbReference type="OrthoDB" id="1708823at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031510">
        <property type="term" value="C:SUMO activating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019948">
        <property type="term" value="F:SUMO activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000011">
        <property type="entry name" value="UBQ/SUMO-activ_enz_E1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF162">
        <property type="entry name" value="SUMO-ACTIVATING ENZYME SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01849">
        <property type="entry name" value="UBIQUITINACT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="7">
        <location>
            <begin position="23"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2070716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="335" mass="37881" checksum="8148D0E73DD62C51" modified="2022-05-25" version="1">MTNDSLGAKEPVNQLTDYEAALYDRGIRLWGVDAQNRLRQAKVLFIGINSLMSEIIKNTVLAGIDSITLIDNHIVTIQDLSCHLFLTHDSIGKKRADESVQSITELNPLVKVHVVDKEIESIDKEFLQAYTIVCLNAFDLNLVSKFDTFCREINIPLIATHSFGLYGFFFSDLSDFEYIKKTEKKEKDQEEPVIEYVNHKVSFPSFETAMNIQWSKTNPRRTSPLLYALTVLYQFEQSTKSIPSIENPSHLSKILEIIDGKLSASQMPSSEQEKYLHITNLLIKHQNHEIAPTCAVLGGIVGAEIIKIISKNNHVLNNIFFYDNTKGTGVVETMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQC0</accession>
    <name>A0A8J4PQC0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carbohydrate esterase 2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007798</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000436">
        <property type="protein sequence ID" value="KAF2070880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQC0"/>
    <dbReference type="OrthoDB" id="30833at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01831">
        <property type="entry name" value="Endoglucanase_E_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040794">
        <property type="entry name" value="CE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037461">
        <property type="entry name" value="CtCE2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052762">
        <property type="entry name" value="PCW_deacetylase/CE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37834:SF2">
        <property type="entry name" value="ESTERASE, SGNH HYDROLASE-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37834">
        <property type="entry name" value="GDSL-LIKE LIPASE/ACYLHYDROLASE DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_2G00620)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17996">
        <property type="entry name" value="CE2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035264208" description="Carbohydrate esterase 2 N-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate esterase 2 N-terminal" evidence="2">
        <location>
            <begin position="33"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="353" mass="38697" checksum="45BC6EFD1C247977" modified="2022-05-25" version="1">MKIYIFISILVCLLVCSCASGLLIAPSDVNVYYVGRVDKSNGSQYVLAWSGVQFSVLVTGTTTFSAVLDSVGETYYSVYIGTAMYVLNISSSTPQPYSISETVSLDPTQTYNITFIKRSEAEIGISTFYGFLVDNGASLLPYPKARGRAIEFIGDSITCGYGMIGVPPCSFQPDQEDITLTYGGIIAQELNAQFYVEAWSGKGIVKNYGSTTIPSNETFPELYPLTIPTQSTDYWDFNDFLPDAVVINLGSNDYEQLPHPSQHLFESTFIEFINYISTKYSDKPTIFLICGPMIGNPCCQYIQNIAVAISAVYIDMQDILKPNDYGCAGHPGVEGHAKMAAIALPIIQKNMGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQF6</accession>
    <name>A0A8J4PQF6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006826</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000332">
        <property type="protein sequence ID" value="KAF2071859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQF6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035147756" evidence="1">
        <location>
            <begin position="21"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="326" mass="36030" checksum="90BD342A477748FC" modified="2022-05-25" version="1">MIKYTLLLFVIISLATSSLAQSAQPIVYATDGQTVDTYQSIDDGNGPVIYFNSVSIENPPKAVFGIVDSNFEQKYIDVLVSRDNVTTVSLSRFILTGYSYDIDQFYLSPFTTSSIIPASFGYDASLGRVFYLTTTPTSIGWPAISLYGAYLGKSKYGTFLNKFSAKSEVALGTYDSSTRTYFAITNTNSTNTYGLQAISYNVVNNQRVVFNVSSSLLLDNNSQVIAVNGRFFIVQATGIIVSVFQADFNTKQVIFVTQSYMGWDPISSFKLTANGKYLSISATQQRYATTDVNMYNLDISPNPYGQFFLNQGTQINTTQSIFVEFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQG5</accession>
    <name>A0A8J4PQG5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008398</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000514">
        <property type="protein sequence ID" value="KAF2070279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQG5"/>
    <dbReference type="OrthoDB" id="1923159at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="610"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="610"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="633"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="649"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="660" mass="75004" checksum="D266D62B93C0AB66" modified="2022-05-25" version="1">MSATTTTTTSTSNLSGGGSGTTTDLVKIYYFISRFLDNDSPNVTSNFKEFLAKEGFMEMLITFITLSYPKTVQDEIDRQKAQGNNSVQSFDPNQSIGSLATAFTSSLSLSTGSLSNSLSASSSTAMTNSLLNNGTPIEVIKSYRVMDLFLHPSTPFISLSKDKLSVIVSSLFEIFKNLDSNYEPKGNLYHFARVLQCLFFQFPFELLQQLSEIQTSSQKSYFQLLSENIYFTPIQDFFLGILKRASVSVSEYQTSNPIDTKKIQKVLQKSKVFNMISNFFLQPETLDIKKEKMEIVLEGFSDFIILVIEELDLHGPDTGIPIFCFQNKEFVDGLIRIMSENRKHSFEHRTFALRILSSFSKQSLEYTNNNNFQLEKDSIFSPSGPSSWISYLSTYHQNTICKIMIDIGNDSSLKKKLSTYRMDTLDLLVEMIKSKQTFKRKTPNQPYLPVELWYFLVDWFFIFNNIFHSKFFDLIKELFLQGHVPSMKHFCNFHLKRFLLFYKNPEEPTDCKGIILLICNFIRISATRFPASHFLPTFLHSNELWNSTLPLIISDTLKNYIGPDAKDDTTVIAYGSDFAKSIGFEDENSKKSTNNKALLIDEFAGGNNNATSSNVGSSSNGNGASFNNNTPEDIARIEKELLMEEEASKKKKKNQNKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQI4</accession>
    <name>A0A8J4PQI4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008361</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000508">
        <property type="protein sequence ID" value="KAF2070324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQI4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="494" mass="56285" checksum="24512EF71FF610E4" modified="2022-05-25" version="1">MDTFYLIWRNQYIRRLIRNIQCRDLLISVKDEYLNDNHQHLSLFTAQNKLDFNISIGYTDTFERYIANQHRNLVNVLSILEGDLDLEQLEGGHVQKLSLKINNDTKICGRLPQSLTYLNIYLKYGQNPPVGQHLLDNLPTSLKTLHLPFHYIPTSKWIVPDTLTNLDYESSFQSLQWLVVPDNRVFKNCVLEIDDSPQEAFEWLHKNKWVNNIKIRCEFTASTIPSHVKTISIKDNCEANLLPSTLQSLSHLALKSLDLLSCPIELKHNVLPKSLERLSIDDYGLPLQPGVLPPNLHTLYLSGFNHPLAIGTLPNSLTKLRIGSYNQPLLPFVLPNKLKKLRMFSYKQPSIGPDTLPSSLVLLDIASFTGTLESFCQPLPLLHKLIINSYNQSLALLLTKTKRLVLWLKTIPNPQKDGTCLNLINTSIESLHLSCSMKSILYPKTLPSTLKYLTLYNVNIKSKGVLPTSCIYLKSVNQTINPDLIPNSVTYIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQT3</accession>
    <name>A0A8J4PQT3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">m7GpppN-mRNA hydrolase NUDT17</fullName>
            <ecNumber evidence="8">3.6.1.62</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Nucleoside diphosphate-linked moiety X motif 17</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_007541</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Acts as a decapping enzyme capable of hydrolyzing monomethylated capped RNAs (in vitro). Hydrolyzes monomethylated capped RNA after alpha and beta phosphates to form N(7)-methyl-GDP. Shows low activity towards unmethylated capped RNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + H2O = N(7)-methyl-GDP + a 5'-end phospho-ribonucleoside in mRNA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:67484"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:17167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:63714"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="EC" id="3.6.1.62"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.62" evidence="8"/>
    <dbReference type="EMBL" id="AJWJ01000409">
        <property type="protein sequence ID" value="KAF2071135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQT3"/>
    <dbReference type="OrthoDB" id="20284at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140933">
        <property type="term" value="F:5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035529">
        <property type="term" value="F:NADH pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019677">
        <property type="term" value="P:NAD+ catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006742">
        <property type="term" value="P:NADP+ catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04694">
        <property type="entry name" value="NUDIX_Nudt17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050241">
        <property type="entry name" value="NAD-cap_RNA_hydrolase_NudC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033716">
        <property type="entry name" value="Nudt17_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42904:SF1">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE-LINKED MOIETY X MOTIF 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42904">
        <property type="entry name" value="NUDIX HYDROLASE, NUDC SUBFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="95"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Nudix hydrolase" evidence="15">
        <location>
            <begin position="252"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="398"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="11"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="32"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="399"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00093205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00093415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00093621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00093663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2071135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="475" mass="53694" checksum="7EBC51473115309F" modified="2022-05-25" version="1">MLRQTHHQQRSNNHNNNNNSNSINSSQDNSLYEYSSSGNHIPQQQHNTSTSIEQSGLPTTTTTTKSTSIIIDTNINFESNSENNSNSNNNKRDKIYVVFLLVLLVFLNLVLLKKINLNYNESSDNINYNMDLSNTNTDSTKARVYWPSVHMIEYNSNTNDNDQQQASLGQWNKCVLKKLGITPITHSKGHTLLELDIESGRLILSPTTDNNNSNGNRKTYPIGHSKECPFHQLERSESIVHKDLQIEKKYPPIKVGVAVLLEDSFNRILLTKRSPLLRIFPNAWVLPGGHMEINESIFETGLRELYEETGVDLIKNRESMDSIDICGVFESAYPVLLPKDKLPVDHHIVVFVNIKLKNDIDPNSIKLQETEVSMGAWIPKSTLSLLLNNTFTEYDDNDSNINNNNNNNNSISSSSSSSSSIEKEFDIISTFQDATKTPDPIPSVDQLKQGFFNQDEKIATGTIFILKQYLNTNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR67</accession>
    <name>A0A8J4PR67_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HssA/2C/7E family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000269">
        <property type="protein sequence ID" value="KAF2072550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR67"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008455">
        <property type="entry name" value="HssA/B-related"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05710">
        <property type="entry name" value="Coiled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="88" mass="7896" checksum="40B86E4499CF69D3" modified="2022-05-25" version="1">MTLISAMSNLSMGSKSISKSAVSGSLITSSQGSNSVACGGCGCNNGGLLGGVVGGVVGIVGGVVGVVGGVVGGVVGGILGGNNGCGCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRF1</accession>
    <name>A0A8J4PRF1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010474</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001136">
        <property type="protein sequence ID" value="KAF2068201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRF1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="90"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="281"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="332"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="347"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="379"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="417" mass="44296" checksum="C77BAD9F360DAD09" modified="2022-05-25" version="1" fragment="single">MMDISVNDNSGKQQHEDDDTSSKRINKANGSSSNLKSILNDKDSNNSSNGNSNSQSNSINNAPSTSSSNLKRNTITFSDNNSLEDLFRYNSNNNNANSGSGNGNGNSTTTGVNSNSSNSNSLNSLANSNRTTSSLHRIERGKSKSSLEDLLRATTDSLNDPIQWRSSTPNRITSSNSNSLDDLWRSFSYSPYKNTSQTTGSSISHLSSFFENDGHTENHHPQSSTTTIPASSSSSSSSSNPSNILLPSLSSSNLHLGSSTTVISSTPNASTLTIPASGHANNNNNNNSSNSSISSNSSTSSSSNNQEEEKQQIVNNKEKEMNKSSLNEKTHNNNNISPPDSPASSPIVNSNTHNLHTSPKSDLESNESNDAYSDASDCSTTNTSPVSPSSSNTDNHHWKQNIASKLPSSSTPNGAMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRF4</accession>
    <name>A0A8J4PRF4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_006991</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000349">
        <property type="protein sequence ID" value="KAF2071691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRF4"/>
    <dbReference type="OrthoDB" id="18127at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06141">
        <property type="entry name" value="WRN_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051132">
        <property type="entry name" value="3-5_Exonuclease_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620">
        <property type="entry name" value="3-5 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF104">
        <property type="entry name" value="EXONUCLEASE 3'-5' DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="4">
        <location>
            <begin position="87"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="566"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="330"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="583"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2071691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="674" mass="76928" checksum="382207FCEBD7540F" modified="2022-05-25" version="1">MKVLENKSNLKNISWSTKIKNTFSKQHEFHNVYNQILNIKTISTSTTTTSNNNSSNITTTNTNTTTTTVLKKEDHRPFFKLPDHLNIFVIKDSTECNQILNELYTSLLNKMGINQDNSNQDENSNNNELMLFPKCNENNNHGLDFILGLDAEWGHTKDGVTTVALLQLSTLTNVYLIQLSQMQYKMPTALQLILQDPRLIKVGVAIHQDALNIFNTFTTITKSCVDLVPLAKLTFFTGNGLATIADSVMNVRLDKNYHIRCSEWGEENLSQEQIHYAAADAWVGLEIFRICFNTYRSRSLDPIKGQDTDCYSFIHPFLNTTFKVKNNPNKKKDSNSNSGSKKENVESLPGYINLKNLIPDRRVLYDNCLMLSPDNVLLCNISKKKVQWYVSRNLADIVNQEPLTIKLRFSPKGNGHADDEFYLANKENRCVVCASDYKLLRHSIVSHCYRTYFPVESKSHSSHDVVLLCFNCHMSVDKRLQIMKMIISAQYQVPIENSTAYIQNHTAFQVSKKALLLKNQLYPDDNTLALSLSNRQKAHAAKIPSDKLYLIKKEVYNYINNINNNINSNNNDKDKDDEDQDDSNSNSSGGDDTNANTSTDMDIDINLTLEQLESLISIATKTKDENFVSFEKKVMDKVLADGEEGLKRFIILWRRNFVETMQPKFLSPHWSVDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS12</accession>
    <name>A0A8J4PS12_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mitotic spindle assembly checkpoint protein MAD1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_006350</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000289">
        <property type="protein sequence ID" value="KAF2072337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS12"/>
    <dbReference type="OrthoDB" id="331602at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.457.60:FF:000002">
        <property type="entry name" value="Mitotic spindle assembly checkpoint protein MAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.457.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008672">
        <property type="entry name" value="Mad1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23168:SF0">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23168">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD1 MITOTIC ARREST DEFICIENT-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05557">
        <property type="entry name" value="MAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75704">
        <property type="entry name" value="Mitotic arrest deficient-like 1, Mad1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="79"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="184"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="307"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="459"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="530"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2072337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="753" mass="86770" checksum="4AF16965763CA225" modified="2022-05-25" version="1">MDDSSDNNSSNNNNLTQQFLKTMNTSSGGGGSMKRYSTSNNNNSFVSRNSGINSKQLIAIQRAIDDYLDTDTGDAGTIIFEANETLARQQEYIKQLEHQIKADNSTENELSTKSVRLTNTEKELQELKSKYYELEKSSQFFKTRASESMIRVQDIEQEMERKIGLKNKEIDDITSKLRYITQSEEEAKKRSLELELQYSELERQTSLKLKAMEKEKLQWRSEVVDLKHKVAGSNEEAQIKSKDSKIDSLNILNKQLQQEVSDIKSRLNSTLELLSDKDRQLESFLVSKEDPILNSLVNPSLSNDEQIKKLQTRLIDSETLNQSIKNELNYYKDVNEKNLKQLSNLKSQLENLGNIEIIKQQNESLQDKLARYQEIIENFNKLEIKHNLLVQEKKDLEKKYGEFDKFQGGPEQFLQRIAILENENQTYVGKIGELTSNLKLLESRNSDIESTILEEKSSLSSKNNRIDEFIEKIKRLETQNYIYKKEIEGLNKILDLFDLDSVVNSNAKEGTDGGDTTAATSDSSVRIERVKELQRALGEKNALVEQYESQLSNKSKAITDHVMDYKSECEKLNKEIDRLLEENALLESRLGKGEYDPTKTKVLHMTMNPSTAADNNKSLENNVGDQKLIEENHRLLMQVNDSEKKLDRLKLVFKQKINEFREGVYALLGYKIDVDQQNRYKLQSMYAEKESDYLMFQYTANKGGRVGNMELLETAFTDSLDREIKAYLFTCKSIPSFLSQVTIELFSRQTFHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS61</accession>
    <name>A0A8J4PS61_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CENP-V/GFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_005168</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfa family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000199">
        <property type="protein sequence ID" value="KAF2073518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS61"/>
    <dbReference type="OrthoDB" id="2993351at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052355">
        <property type="entry name" value="CENP-V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006913">
        <property type="entry name" value="CENP-V/GFA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28620:SF1">
        <property type="entry name" value="CENP-V_GFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28620">
        <property type="entry name" value="CENTROMERE PROTEIN V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04828">
        <property type="entry name" value="GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51891">
        <property type="entry name" value="CENP_V_GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CENP-V/GFA" evidence="4">
        <location>
            <begin position="9"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2073518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="142" mass="16474" checksum="C532D4437AB715DD" modified="2022-05-25" version="1">MTSNKQQTLNGYCHCRTVHFQVLVDLTKPLETIKCNCTICAKSRWWEFYVHPEEFTLISGEDQLGLYQFGDLQTSHYFCKRCGVKPYIKGTSGNWGGKFVGINVNCIDEFTQEQLESFTPNISYLNGISNDWNHCPKHFKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSD1</accession>
    <name>A0A8J4PSD1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4286 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006160</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000272">
        <property type="protein sequence ID" value="KAF2072517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSD1"/>
    <dbReference type="OrthoDB" id="17786at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025563">
        <property type="entry name" value="DUF4286"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14114">
        <property type="entry name" value="DUF4286"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2072517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="132" mass="15132" checksum="55968743455FC6B2" modified="2022-05-25" version="1">MDGPKASQLIYEVSVQVDKKIVVEWEKWIVEHINQIVSLDNGTLFSKGHLCKLDQSFDSANETDCSHIGFVIHYYSNSKQCLNTYFDKHAPLLRKDGLDRFGDQFKATRRVFVIEKEITAPPPQSSSFGYVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSI8</accession>
    <name>A0A8J4PSI8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_005977</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000255">
        <property type="protein sequence ID" value="KAF2072717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSI8"/>
    <dbReference type="OrthoDB" id="2129491at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051165">
        <property type="entry name" value="Multifunctional_ANK_Repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123:SF33">
        <property type="entry name" value="PROTEIN HOS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13405">
        <property type="entry name" value="EF-hand_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="234"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="267"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="342"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="375"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="9">
        <location>
            <begin position="481"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="622"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="658"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="129"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="535"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="744"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="443"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="131"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="536"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="552"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="746"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="783"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2072717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="783" mass="88145" checksum="55579F99A3FE8660" modified="2022-05-25" version="1" fragment="single">MTICSPIAAGNWLFWNSLENFKNKVFILQAYSGPLWWTDKFRILLLIDSPNTSNGYLIASSSSMEEIITNWDIIQTIYQEKVTKTGLSQFSERFLLDLFKKLSKKMNRAIQTAETIELNANNNNCGASNQTSFSSMKSSTSGFGTSPSTKNHHSNSINSSSSGSNSNYNNNNNDLYISPFFLSKDSVTIQLGMQRKVALLFKALYHTEPEQIIEKTFARVFPNNEWIEWVDEKSGMRPIHVAVERMDINLIKYLIEKRADVNIKDLQGWSPLHFSSFAGNLDICQLLIEQGNAAVMSISKDGTLPIHYLARHSFDPILLPKFTMVLESLVSKGTPIDAKTLSGESALHRASYSGSLQVVNFLINHNANLNIQNNRGETPLYFAVIGKHRQIIKTLVENGADCSLGGATGNALTAAKRMDQTELLCFLAGFAKSYFSDESSGYDQQDIEEIQQAEKERKQKEQEDSAKQQLYQKQLNTPFFPHVFVSIDFRTNNWCSYCGYFLWGIRKQGFSCEICGYCVHPKCKKKATLTESCLGNSSSSSNHNHSSLTSSKRSLTESGSSRSVDDYLSQSRDLQYQPIHKQNINRKRLESLYNHFNTLDKDQKGIIYKKEFSESLGSILSDTNSFSSALFLAFDSNKDGKMDLRDFLNGVSILQNSSLDQQISFAFKMLDKDKKGFITVQELVTILESICESLSNLKILSVNPIDFVSTLFPHFLQDSSTSSPLFSPSPPSKKPVYSLFQSISLNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNYMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT77</accession>
    <name>A0A8J4PT77_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_005838</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000243">
        <property type="protein sequence ID" value="KAF2072861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT77"/>
    <dbReference type="OrthoDB" id="432299at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004128">
        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000001">
        <property type="entry name" value="Cytochrome b5 reductase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051872">
        <property type="entry name" value="Cytochrome_b5/Flavoprotein_Rdt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46237:SF1">
        <property type="entry name" value="CYTOCHROME B5 REDUCTASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46237">
        <property type="entry name" value="CYTOCHROME B5 REDUCTASE 4 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="5">
        <location>
            <begin position="64"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2072861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="145" mass="16760" checksum="78DBA8F5732589A0" modified="2022-05-25" version="1">MDAKTHQKLVELYKSVELLPTDQEVEDKKNGKRKRKVPLQHGHSQLDWVKKHNAIQSNTLYQGHGKIPLSEFKKHNTVQDAWTVYKGAVYNITEYFTYHPGGDTELARAAGHDCTKIFEFTHSWVNFESMMAKNLIGYLIPDDMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUW2</accession>
    <name>A0A8J4PUW2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBL3-like ubiquitin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005932</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000251">
        <property type="protein sequence ID" value="KAF2072744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUW2"/>
    <dbReference type="OrthoDB" id="20687at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039540">
        <property type="entry name" value="UBL3-like_ubiquitin_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13881">
        <property type="entry name" value="Rad60-SLD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBL3-like ubiquitin" evidence="2">
        <location>
            <begin position="9"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="346"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="347"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="365" mass="42134" checksum="C52FB2471C5EFDB1" modified="2022-05-25" version="1">MSNTVDRNKVSVRFIIDKSHQYVVLLDRSTTFKQIEHQLLETKPNDLFSPTIASNLDDYQLKFIYSGKIFNYQSTVADLEKTQSKYPLNVQLIHLKKSNNNNNSSNSSNSSNSNSSQEEETFHAHGCFFDIDEFQQIEQIFNSKENHDNHTVDLSFLNNFLHSYWTFLISRHPTLLENSPSFNSNRNVPSSSSSSTTNTPTETVNTNTNTNTNTNNNNSSRPFPTEKLSKILRTVLKVNSLDAHTRINKEQFRIIFYLFTTESDNHACPSGSIESVKRMIQQYHVTLDTDHPFDNEMFNTIYSTVENLQTRNENQQQQPLTCQNIELLFYLYTAHVFIQRDKHLETNQDQQHNQHQPSSSGTPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVC8</accession>
    <name>A0A8J4PVC8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MINDY deubiquitinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004399</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000154">
        <property type="protein sequence ID" value="KAF2074305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVC8"/>
    <dbReference type="OrthoDB" id="10261212at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016807">
        <property type="term" value="F:cysteine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990380">
        <property type="term" value="F:K48-linked deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071108">
        <property type="term" value="P:protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007518">
        <property type="entry name" value="MINDY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033979">
        <property type="entry name" value="MINDY_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18063:SF9">
        <property type="entry name" value="DUF544 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18063">
        <property type="entry name" value="NF-E2 INDUCIBLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04424">
        <property type="entry name" value="MINDY_DUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MINDY deubiquitinase" evidence="1">
        <location>
            <begin position="100"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="402" mass="45827" checksum="19D60AF4F58989C7" modified="2022-05-25" version="1">MTSKVPPKESFLYKAYNGSTLEFDIAGDTCQKFGFFHGCRVQTPKGLASVIGVRDGNLWFHVDGDPGASYWDNGKDYEDLVFKLQIQLIDDFPLEVIENKYRVKRINYLNNEVSIILQNENGPCPLISIANVLLLQRKVSLDPDTQSVTIKKMGDLIMKHAKTIYKNDPDVLAILEDYDKNVLPTLESGLIVNILFNSIFGFDKTAPCQIFDYLHIKLVHGWIVDPEKKELFKAIGNQNYNDLTPKIVTFDQSFPDSPKELEQEIKDFANSNQLTDYGLSLIQQNLKEDELCVFFRNNHFATMTKHDGFLHILVSDLGYEREKNIIWDRIMTKEGESLFLSGKFLSRKDESIIEVKSTLVLFGFSTPQVDEAIVHISAIDKLDVDLLDEATKYLTSKGYIPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVC9</accession>
    <name>A0A8J4PVC9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000196">
        <property type="protein sequence ID" value="KAF2073572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVC9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="426"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="382"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="487" mass="56626" checksum="8A1B3F1425ABA4BB" modified="2022-05-25" version="1">MDTTRDSGDDFDSYININNKALLTAQFPELFDKENFFLDEKYKPQVPENDSEYVDHDMALLFVILWAINHRDGCTIDNQVTTIDLDKDYKTVVATYPSWKITKDRFAEICSEVNPDASESPPIHFFNHSKFNNFFIPCIRENGVNSYCYHAANDTYARLVISVCHTKTNNKHQPRQTLGTYENDFNTLNLRLNPYGQNLKKFINGCKRCSDPAHKSMDSSQSSFLKDHNYVQQTQESTLSEMIEPESNSMEDQEINNNNKSVVDKVIQEEKNSGGQPSIFTIPSPTNNLFQSSLPKIANDTQSESMMVSQSFLYQQQQQLVQQQQFQLFQQQQSKINQLEYIQQQHQLEINNLLNREKERSEKKKLKNKERRQRKKQKVLQEKNNNISSNSNHPIIETNNNNKEMNNENGDIVFESEASWGAEISLNDLSTTTSTNHQQQAKRKRESMSSLHTTTFAEYPHNSFFSHYPERVVVKKEKQSWRDCAIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVJ0</accession>
    <name>A0A8J4PVJ0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002935</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000087">
        <property type="protein sequence ID" value="KAF2075753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVJ0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2075753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="252" mass="28697" checksum="ECAA2D7FEC3BCCD4" modified="2022-05-25" version="1">MGNRSSTSSVDLKRKGKERGSSFIQNSTYQSVAIFTKDGVFPISRVRKNIHPPDKLMKSNKNNLCAEKIGRLYLCQKENGLSINTKATHHFSLVLDVGGHSDGNNLRGICMHIAFSPKDKNMIYIQQPIDALKDYDFNSVTDTGGLVYPHDNIEHLKRLSTGHPEFSEMDMSADKNDLWYYYIVFQMDDWLSEFVETYGRTFSANCNCQSFAKFLHKKLSGVEFPNTPIFGQSSFDKFTYSAYSTITQTFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW76</accession>
    <name>A0A8J4PW76_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_004803</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000177">
        <property type="protein sequence ID" value="KAF2073879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW76"/>
    <dbReference type="OrthoDB" id="18487at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF5">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035265627" description="EGF-like domain-containing protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="990"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="733"/>
            <end position="744"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2073879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1034" mass="114565" checksum="F2C22A97B8CCE0BE" modified="2022-05-25" version="1">MMRNYKHCIVIFPFLFLYLHVTLGVAPIIEGFKPSFGTVGTSDRITILGSNFATLSAVYSNGIKQSVITSIDSTTRYFTNTAPSSLQYNIQAENQAGEKSNIATYYIAQVDVANSYQKNTDYYIVGTFDTIPDHSNLKVMAILNYPLQYLNGTHLTFKLNLELLSANSIGLYDDSTSVSVESFKFEFAPIVHSANFNMDLLYIQGIFLETNNLEISILTRGVVYPCVPSAASTSDMSCTATKRSLTNPITSFQILNKRNSKVTYYSVVFQTAIQSHSNFANGTAIFYGQFSIDLNNKLDGLTNLNINIDDIKPTQFTIIYPANAKCGYAYVGTAGLRQTNNYYVCPTPKLENVITLPSTSNGLVLTFKSYFTSMVVYGTNDNAIVFILDYQDGQDGYMCAIADSKYASGSYESTCVVPKENSFFIVAVTYSGEISNRLLVEYKPTISNCSSTDYMVPGKVTIYGTNFINQNIKISIETANCINPIPRNATQIVCNFPSNIVVQDFFKPLQVTISVGTYQISENIFLYNRPNPLIQSATSILYGSPGLVTIVGNYFGDPDIVVRIGGSDCRVTILTDTEMVCSFNSDVPSDYTTPLDVFVSIRSKYNSISRVFYYTRPTPIIKSATSTKYLVPAKVTIIGSILASDQIFVSIGGSECSQALSSLDGTSITCQFKSDVFIDDFRKPLDVFISIEQKFNSTNQVFLYINPAKNCPNGKNGELCSGHGQCNQQQFVCDCKDGWASFDCSINDGGTIIPDPNVNNDTSSTIITPSGTEFDVGIILINEMGQDDNILNSYNVGNITWNNITKQDNQYSYMTTLPNTNSTLLVKLSINPLDKRVFYNFAGDIIPILPKSIKYQVELENYDFKSTLNSLNFIFKSGVTKGESNCIYQENTDTQVLLDSLRSIQMTLNGETLVGTFSDRIILDNRPSYNKVEKLNQTEISKLSLNDQITYISISTSYFKSNVVVDPNFGVLVSNAPNNGCMEKKKFESWKIGVIVTAGVIAVSMAVAILYVLKKRKQRILFNAKLQKLNNNKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWX3</accession>
    <name>A0A8J4PWX3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003506</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000110">
        <property type="protein sequence ID" value="KAF2075201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWX3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38742">
        <property type="entry name" value="PROTEIN GP17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38742:SF5">
        <property type="entry name" value="SECRETED PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035196065" evidence="1">
        <location>
            <begin position="27"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="197" mass="21663" checksum="0A1C7E729A80D215" modified="2022-05-25" version="1">MTNTKTIVSIVIVLVLILNFSSFIEAKKQKTKDVGKLSPDNAIDFLYGAFKGIEAGVNPISDNCFNYTDPLVITLKSIASSIIGTQELSPSEINAAILDLISAIQDIIPNIDSCVVPQILDRITEILTFIKDKGIRSFVAKETSVLIINKNKVVENFRNFFVHWNQGKFGDAGRNIGDVLGVFLQSPYGKKTKKISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX00</accession>
    <name>A0A8J4PX00_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Leucine-rich repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003259</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000100">
        <property type="protein sequence ID" value="KAF2075430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX00"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="245"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="252"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="332" mass="36170" checksum="E8B21516D3D1DAA9" modified="2022-05-25" version="1">MGAKLAKSLDKAKENGVTELDLVDKGIDDLPTNIGTVETLKKLNLSKNKLKKLPSTIGNLRALNVLNLFNNQLKELPHEITLLTNLESLNLSVNKLRSLPRGFGSFNHLYFLDISYNSITEISTQIGLISTLKELHCSFNELTELPPDLSRCTDLEVINASHNRITELPADFHKLPKLKLINVTNNKIQWIPPAYGKLEKIEKIDFSKNPVNKTLDEKAIRGPEVLIKYLSSDEYGSLYLAETKETKEKETAAAAASTTTTTTTTTTTTTTTPPVASSPPKETSTPPKSGEESPAVSESPPTPTQVSESLPPPPADTPTELPTEIPGPSAGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX53</accession>
    <name>A0A8J4PX53_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">adenylate cyclase</fullName>
            <ecNumber evidence="4">4.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">CYY_002213</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP = 3',5'-cyclic AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="EC" id="4.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.1" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000059">
        <property type="protein sequence ID" value="KAF2076473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX53"/>
    <dbReference type="OrthoDB" id="6147412at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
        <property type="term" value="P:cAMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF12">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0115">cAMP biosynthesis</keyword>
    <keyword evidence="13 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="581"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="605"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="636"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="687"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="720"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="17">
        <location>
            <begin position="216"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="17">
        <location>
            <begin position="800"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="429"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="441"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF2076473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1007" mass="112931" checksum="152B46C089BE18E4" modified="2022-05-25" version="1">MSNKHSYLKGCGSPNKVSALTLTNSCYGTSYSHNSPLHATNNTNTSAAATFNSSQVSSTLSKSNTIDFSDMHMDCRNRSESVANTLNTHIDIENPQVWNDNPMILVNKDDTYSKKNKNLFQKYTLKLPNKLENEYLDHHFNSQIMLLRAANLLGLLSSFVDEKRLQKKAGCLKEEKEKSEQLLSNILPDFVIGMMNKEWIMHRSSQPISKDFPECTILYCDIVEFTSLASGLDPESLVKLLNEVFTEFDKVVEKNGCEKIKTDGDAYVCAGGLTIKNNSHYSAVIDTAIDIIALPVLKSNYLQKEIKLRFGVAQGSVIGGVIGVQKFQFDVWGEAIAMAHTLEQTGSPGRIHIVEAGIEKLSTSKYSLEENVDSHGHFPFPTYFITYKKPDEDKGVDENDIDINNPKTALIDKSLASLKEDSIPLNQSIDGNITRFPKSPSLMSSPQSLKETHSPSTDSCITEEGATLNTSDSPLSLNVSVNSKEKTKLRDKKQLSSPTVSGANNISIDMKPLRKKRNKFLRLLFGIPKETENVANEISEMDKDFHEYTKLRKLIVQFKNLFVEREFHKYVIRNTIIETKFFLIVGAVLHMVFFIDDWVMKSSSLYNTSAIYSCMGALFGLYLLGSFSRFVKKPLVYQITVFLLLITFGICTILELMRYDNPLARSSLTRVCAILFYVNVFHNLNFLVVLFINLFIFSFFFACATALSPNLLNHIYATDYLGIAIVLFIQVSSSFGMKLAMRKAWVIKSKVKFKSLVLAKEREKSAKLLENILPQSISEKLMSEGKANGMLIAQNYNQVSIMFINIAGFEKLPESLEASAMISKMNTIFSTFDELVMARSDIEKIKTIGTTYMVVGGLNGSLPRNYLYNMAEMALTAKSLVHHFSKEHLNVQIGISVGPIVAGCIGIKRAKFDVWGDTANIASRMQTSAPPGKIQVTKEASKILRKGFYVEERGTIKVKGKGDMQTYFLTARKGSATDFEEENLPAHWKQKLLQFSGSYIKEDEFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX59</accession>
    <name>A0A8J4PX59_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase C39-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004192</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000144">
        <property type="protein sequence ID" value="KAF2074487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX59"/>
    <dbReference type="OrthoDB" id="293230at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039564">
        <property type="entry name" value="Peptidase_C39-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40524:SF1">
        <property type="entry name" value="PEPTIDASE C39-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40524">
        <property type="entry name" value="PEPTIDASE_C39_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13529">
        <property type="entry name" value="Peptidase_C39_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035229263" description="Peptidase C39-like domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C39-like" evidence="2">
        <location>
            <begin position="26"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="181" mass="20299" checksum="1BDBE093D559976B" modified="2022-05-25" version="1">MLYKSILLLALFVGTIYCNIVLPYPYPLYKQCDSRWANNVIMNETICDVGCLMSSISMSIAGRELSINGQLSTPATLNAWLKSNNGYTDGDLLIENAIPNISPVIQWVGSYEHSDFTIQDIQDMLDQQIVVILNVNNGGHFVLAIGYDETNDTIYVNDPGFNKQSYTYEDAVGYRIFKIMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX75</accession>
    <name>A0A8J4PX75_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ADF-H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_002196</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the actin-binding proteins ADF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000058">
        <property type="protein sequence ID" value="KAF2076518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX75"/>
    <dbReference type="OrthoDB" id="25672at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11286">
        <property type="entry name" value="ADF_cofilin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017904">
        <property type="entry name" value="ADF/Cofilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11913">
        <property type="entry name" value="COFILIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="5">
        <location>
            <begin position="2"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2076518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="136" mass="15717" checksum="532890277DA0CA1D" modified="2022-05-25" version="1">MSSGVTVNPQTIEVFNQLKLGKQCNCIFYKLNDDDSEIVIEKVLPNSTTFEQVVNELPPNDCRYVVYDYDYEIENEGKRNKIFFINWCPYSTPIKNKMIFTSSKESIRRPLVGIHAEIQSTDYSELSKDILNAKCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYG1</accession>
    <name>A0A8J4PYG1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exocyst component Exo84 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_004313</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EXO84 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000150">
        <property type="protein sequence ID" value="KAF2074379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYG1"/>
    <dbReference type="OrthoDB" id="642193at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1220">
        <property type="entry name" value="Exo84p, C-terminal helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1210">
        <property type="entry name" value="Exo84p, N-terminal helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033961">
        <property type="entry name" value="Exo84"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032403">
        <property type="entry name" value="Exo84_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042561">
        <property type="entry name" value="Exo84_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042560">
        <property type="entry name" value="Exo84_C_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21426">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21426:SF12">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16528">
        <property type="entry name" value="Exo84_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst component Exo84 C-terminal" evidence="5">
        <location>
            <begin position="220"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2074379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="783" mass="89708" checksum="7DAB76AE9320E4DB" modified="2022-05-25" version="1">MKKGVTYALKIDNQLSTAGSSVVYQDIECLDIRANGIYVVKFMFREWVGPLNQLTNPKSSIKLAINPKEFLVTCNHSDKKKTVYRWDTKPSEVKSAEDNPIEYTTFSTGSFNVDKYTTDLFTHKTDTQAEVHLNYLNERKLGCIDHLKKDVYKNHLIFIGASKEIANSEVDMLDFRNLVTDYGNCITSLQNLSINWDHYKVKKNNKNEFESLSSTTEPIQWLTTAPNELRVAIQQREFETAVGFVEKINQIYESNPKVDITMQTHPLKESIENNIKQLTDKLMDELRSPLLKPNQIKETISLLVRLSQNDKAKSIFLESRSHSISQAIKKVPVSGDLNRYIGELVRIIFNSIIATCNDFTNSFPSHMNSGLIGWVIDELVLISDIFQRQVFILDNFYAIAQTMRIVESHCEMMDTTGLSINFYWKLLLQPHLEQVITGYEQKIRNQTRHHLSEEKFIGISKWDYEIQITSPNLNKDATPSKNTPYLSPAQKAVLEAQQQAQQAQLENDDSKLKLTQSTIFLNSATQRFANDICQIVSIDSIPVVSVSLSKIFKEYMTHLLAQMEKGTLNDNQCLSIISNSIFIVDDLAHRIANKFEDALGEKLNNLKNLSEELLYLHESIRDEYAIAKARDIMSDHVMNWDEEDYRIEEEIEAFPKKFIKLSDHLDTLANTIQMNVNSETVLPIISRIISEVVVLISQRLQANPVVFGYGGLQHFVLEMKYLTTFAGKYPVEDFTFGLISDLIDKNVDLFASTNQFDSSTVLKPDEYFTTIIDNLVYQKAVYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYL2</accession>
    <name>A0A8J4PYL2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Myb domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_001141</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000026">
        <property type="protein sequence ID" value="KAF2077523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYL2"/>
    <dbReference type="OrthoDB" id="270417at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02335">
        <property type="entry name" value="ZZ_ADA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000110">
        <property type="entry name" value="Transcriptional adapter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000087">
        <property type="entry name" value="Transcriptional adapter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.90:FF:000008">
        <property type="entry name" value="Transcriptional adapter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041983">
        <property type="entry name" value="ADA2-like_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055141">
        <property type="entry name" value="TADA2A_B-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374:SF20">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 2-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTOR 2 ADA2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22941">
        <property type="entry name" value="TADA2A-like_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25299">
        <property type="entry name" value="ZZ_ADA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="11">
        <location>
            <begin position="380"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="13">
        <location>
            <begin position="438"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="14">
        <location>
            <begin position="442"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="10">
        <location>
            <begin position="442"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="SWIRM" evidence="12">
        <location>
            <begin position="746"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="507"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="708"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="16"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="106"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="149"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="174"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="210"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="223"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="259"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="290"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="307"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2077523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="835" mass="93828" checksum="81BF027B193A33C3" modified="2022-05-25" version="1">MKGDKTVSMDKDDDLNNNSSSSSNSSNSNVENKTETTPTPTLEDLPPTQPMGEDEEDEEEDLMINKKRNRESTITTDEEMSESSLQTSGKGKSKRRASSSNGGGTMTATTTTNSNNSNSNNSIQSEEDEIVSTRNNRRRARKKQPQLLSDEDDDDDDEVDDDTIDDVHGGGLGGEEDDDDETNEDTSAVDEEDGDEEDDDDTSSVLSKSSSRKGFNRRKQSAKKQQFGGSARPTRSNSASLDDFDEDMVDTNSARGKEDSDESYAESEDDDDDVDDESEEESDDEDGSSSKKRKSKLIKKKEVVKRSSPSTPISSTATASPPQSKTTSTTTTTTTTTTSTSTPNLSDTTIKESTTSPPKTPKKKSPPITSPVPEEPKPNTVLYHCDYCQKDISGVVRIRCSVCQDFDLCLECFSVGVEITPHKNDHDYHVIENMHFPMFTDSWGADEELLLLEAIELYGLGNWNDVSENVGTKSAPECKTHYFTYYLNTTTSPLPDTSNVLTSSENVQFKRAKPTTPPLTSDRINKDRNKSKIGGKSVNEEGPSGPVTDAVGFMKNRGHFEVEYDNDAEIVVKDLTFDAEDTLEDREIKLQVLESYSQRLDERIRRRNFIIDKGLLDYKKVERKRYKDDKEIVNSLKVFLQTIDKDEYETLLDGLITEKNLKNQIAQLQKYREAGIKSMTEGDIYEEEKRKRELERNTKKSKSDLSYLSSVSSIDKPTSTNPNHFKSPKQYTKEKEDTFLGYERKSMKLRKNAQLEMEGLPNSEVLSTKEKLLCTAIRILPQQYLVIKETLLAESLKTQGIIKQSVANKLIKLHQTKVIKLLEFFEQSGWLKRQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYL4</accession>
    <name>A0A8J4PYL4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Rab GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_003325</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000103">
        <property type="protein sequence ID" value="KAF2075349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYL4"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000067">
        <property type="entry name" value="ras-related protein RABA1f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2075349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="215" mass="24148" checksum="4A6A212515C635DE" modified="2022-05-25" version="1">MSKSGSQEEYDYLYKIVLIGDSGVGKSNLLSRFTRNEFSLETKSTIGVEFATRTIETQGKVIKAQVWDTAGQERYRAITSAYYRGAVGALLVYDIAKQATYKSVERWLTELRENADRNIEIMLVGNKSDLRHLREVSTDEAKEFSEKHKLSFIETSALDSSNVELAFQNILTQIYQLMSRPSHGPIVTDDPTPTGVSVDWNNNNETATKPSNCSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ88</accession>
    <name>A0A8J4PZ88_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_000744</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000015">
        <property type="protein sequence ID" value="KAF2077943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ88"/>
    <dbReference type="OrthoDB" id="534912at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097272">
        <property type="term" value="P:ammonium homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3430.10:FF:000008">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730:SF6">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="210"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="301"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="331"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="375"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="9">
        <location>
            <begin position="21"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2077943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="434" mass="46568" checksum="603F99B418DA3762" modified="2022-05-25" version="1">MEYLELNSTKEGLASAVDDMWVLNATYLVFYMQAGFCMLEAGVVRAKNAKSIIMKSIIDTAIGSLLFWALGFGLAFGNANKASNSFIGTSHFLLIGYENLSFFAFQWAFCATSITIVSGSVAERVHVNSCLVYTIVMSALIYPFSAHWVWSYNGWLRNIGYNGIIDFSGGIVVHIVGGCVGLVGAYLVGPRIGRFDSDSGKPKPLPGHSITLSTLGAFIIWYGFYGFNTGSTLGVSGGRINIASRASVTMTISACASCATTLLAIKIKSGKYDVIKSVNSLVGGLVSSAACCAMVEPWAAFIIGCVASFVYLGGSNLLLKLRIDDPLDSSAIHLGCGIWGALSVGIFATERELSLILRKETTVYGLFLGGGFEQLGIQVLGITVVILWCLISSCILFLIMKKFNLLRIDPTKELMGIDMDHHGGPAYPNNIYVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZD3</accession>
    <name>A0A8J4PZD3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000062">
        <property type="protein sequence ID" value="KAF2076405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZD3"/>
    <dbReference type="OrthoDB" id="66510at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043291">
        <property type="term" value="C:RAVE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028241">
        <property type="entry name" value="RAVE2/Rogdi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13618">
        <property type="entry name" value="LEUCINE ZIPPER CONTAINING TRANSCRIPTION FACTOR LZF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13618:SF1">
        <property type="entry name" value="PROTEIN ROGDI HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="369"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="210"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="369"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="456"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="477"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="487"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="572" mass="63523" checksum="8A2551D5C6626C1C" modified="2022-05-25" version="1">MDMEDQDIDHDMIAYELLQMQNESYWLIKTEVPTVCKRVKRILNKCIDFLKPLEPVTEQEEDKDKDAEQQDKGGVSSPNITSTTTKKKEEEIEDFEVLTGVEIQEDTNEVLKDSSKRFETLEGSRGIVNLNGWMVDSQEFVIKFLKQPHHKSNQPIPTYKTSIQKENPWRIQQIQNSYNHLGFIIAELDNIISFSENFVPPSIYNLPTNGNVDNNENSNSSNNSNSSKINDSMTGMITSTTDDNASTTSTTSTTNLNQSNEYINAAVAPPLSSSQTFIPTPNNIKAIQNSSVVRSPELISLVENIKSISEWLSMAKEELLLPSRNIFPSTLYLPNVLQPPLPFEINVDISLSNCDLLISIHELQISSVESTPSSSSRFSPNNDNSPSKFLRRSSTTITSNNSTALSSSSSSLPSSQSNTPSKSSPIHSSAHHNNQSSQNLSNGKGDQNNSGMGQRNSSNPNLLSLSTGNISVNGNGSLSPTSSSLALHHHHHHQHHNHLYHGDNGETLYTPDYMKKPFQTNKGGSLQWINVINHTEARIPMRQLSDSFALLTSAYDKLADLSEKFMVISNIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZD4</accession>
    <name>A0A8J4PZD4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">pantothenate kinase</fullName>
            <ecNumber evidence="4">2.7.1.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_001697</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-pantothenate + ATP = (R)-4'-phosphopantothenate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16373"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29032"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 1/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the type II pantothenate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.33" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000042">
        <property type="protein sequence ID" value="KAF2076988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZD4"/>
    <dbReference type="OrthoDB" id="498611at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004594">
        <property type="term" value="F:pantothenate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd24123">
        <property type="entry name" value="ASKHA_NBD_PanK-II_Pank4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000025">
        <property type="entry name" value="pantothenate kinase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004567">
        <property type="entry name" value="Type_II_PanK"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00555">
        <property type="entry name" value="panK_eukar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280:SF20">
        <property type="entry name" value="4'-PHOSPHOPANTETHEINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280">
        <property type="entry name" value="PANTOTHENATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03630">
        <property type="entry name" value="Fumble"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="519"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="12"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="94"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="520"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="547"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2076988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="587" mass="64612" checksum="DC7601232E3C82C6" modified="2022-05-25" version="1">MINLRGACMINDDNTPPLQSVNQIKPRQISTSSEDNRSRSISGSEQGVPIMIERSNSHVENLSSSSSSSSSSSPLSTSPTTQSSSDKQPINIDNLSDSLLKTNLNSSSSSSTSTTTTTVAPTTNNNININTNTNTNTPSSSPPKYSPKDRPISLPNQTSPIKHFALDIGGTLAKLVYFVQNKHSKNRHNEMGGRLHFIKFQTANIEDCFNFILENNLHIDEGKPKVVRVTGGGAYKYADLFEKRLGCKLVKEDEMQCLIWGTNFLLNNIQKESFTYSPLPNCPSPKEFVDKVENPYPYLLVQIGSGVSIIKVDSETSYQRVDGTSLGGGTFWGLCSLLTGVTDYDEMLELTKHGQSNAVDLVVGDIYGTDYTKVGLSSDTIASSFGKIIYKNASEKQPYRREDIAQSLLKMVANNIGQIAYLNSQRYGLNKIYFGGFFIRDHPNTMQRISFAIDFWSKGKTKAMFLVHDGYLGSLGAFLAPDEIVGSANSNSGNSNSSGGVDNSTNWWKRTTRELSKIFNNNNNNNLNSNSNNNSENNSNNNHNSNHHQQQYHHSIDNSSNNDLDCECDCGCEFCEGLVCKVDLKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZI9</accession>
    <name>A0A8J4PZI9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tubulin folding cofactor B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002220</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TBCB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000059">
        <property type="protein sequence ID" value="KAF2076480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZI9"/>
    <dbReference type="OrthoDB" id="2130750at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043014">
        <property type="term" value="F:alpha-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007023">
        <property type="term" value="P:post-chaperonin tubulin folding pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01789">
        <property type="entry name" value="Ubl_TBCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.190:FF:000013">
        <property type="entry name" value="Tubulin-folding cofactor B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045172">
        <property type="entry name" value="TBCB_Ubl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916">
        <property type="entry name" value="DYNACTIN 1-RELATED MICROTUBULE-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916:SF85">
        <property type="entry name" value="TUBULIN-FOLDING COFACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14560">
        <property type="entry name" value="Ubiquitin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="5">
        <location>
            <begin position="31"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="CAP-Gly" evidence="6">
        <location>
            <begin position="202"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2076480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="29170" checksum="C782EBDF2D19DBEF" modified="2022-05-25" version="1">MGSDYEVLRGYVIGSGSDSNQQKAPEGQVRLEITHSVLSDDTLGANNYKNFHLNLTVKEFKEKLYRFVGTEPKYMTLVIREQNKITEIKKLDDEEKTLEQYQIQDGLNVHIIDNDPNNYISELQDTSKVEKATMTDEEYNKREGTYKKWIEEQQKSNTNTSTATTATTSTPEVTIKVGDRCQVTSDDPNNYDIRLGTVSYSGTTEFSSGHWIGVTLDLPLGKNDGSVKGKRYFECSPKYGCFVKPSNVIVGDFPEEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZN6</accession>
    <name>A0A8J4PZN6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Esterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001538</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000037">
        <property type="protein sequence ID" value="KAF2077153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZN6"/>
    <dbReference type="OrthoDB" id="30833at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01831">
        <property type="entry name" value="Endoglucanase_E_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040794">
        <property type="entry name" value="CE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037461">
        <property type="entry name" value="CtCE2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052762">
        <property type="entry name" value="PCW_deacetylase/CE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37834:SF2">
        <property type="entry name" value="ESTERASE, SGNH HYDROLASE-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37834">
        <property type="entry name" value="GDSL-LIKE LIPASE/ACYLHYDROLASE DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_2G00620)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17996">
        <property type="entry name" value="CE2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035316309" description="Esterase" evidence="1">
        <location>
            <begin position="22"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate esterase 2 N-terminal" evidence="3">
        <location>
            <begin position="33"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="SGNH hydrolase-type esterase" evidence="2">
        <location>
            <begin position="153"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="354" mass="39224" checksum="FB9669B3CBC8902B" modified="2022-05-25" version="1">MMTITATLFFILGVLTLCTSGLLIPPTNENIYYMGRIDKSDNTQYTFAWSGAEISILLTGTTSVSPVLDSTGQNYFNVIIDFTMFPMLNVSSSSPQPYNITETLSLDPTETYNIILTKRTEASIGLINFYGFEVDDTAQLLPYPDIPRRSIEFIGDSITCGYGDLGVPPCEFEARTEDNFMTYGALIARELEAELYIEAWSGKGVVRNYQAPNITSNGTFPTFYPLTIPTESTNYWDFSDFQPDAVVINLGTNDFSSEPIPPQDIFQNGYISFVNFIKSKYTNNPTFFLACGPMIGNPCCQYVQNVATTVNAIYIDMQGILNTNDYGCNGHPNVSGHSKMASIASPIIQKNMGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0C6</accession>
    <name>A0A8J4Q0C6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Saposin B-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_000103</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000002">
        <property type="protein sequence ID" value="KAF2078603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0C6"/>
    <dbReference type="OrthoDB" id="18161at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.225.10">
        <property type="entry name" value="Saposin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011001">
        <property type="entry name" value="Saposin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008139">
        <property type="entry name" value="SaposinB_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47862">
        <property type="entry name" value="Saposin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50015">
        <property type="entry name" value="SAP_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035278634" description="Saposin B-type domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="3">
        <location>
            <begin position="22"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2078603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="107" mass="11685" checksum="05B56D5F2B3D5259" modified="2022-05-25" version="1">MKFTSIFLIVLIVLSCAISSFATPQCEDCKNALKTADGKLPAKRTRQVVENFLQQNCQTATGLVPIEVPVPLPVKLCDAMAAHKSDFVNGLIERKEYTLVCKKAGLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0H1</accession>
    <name>A0A8J4Q0H1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">U6 snRNA-associated Sm-like protein LSm2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_002214</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Binds specifically to the 3'-terminal U-tract of U6 snRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000059">
        <property type="protein sequence ID" value="KAF2076474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0H1"/>
    <dbReference type="OrthoDB" id="10256176at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01725">
        <property type="entry name" value="LSm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000009">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016654">
        <property type="entry name" value="U6_snRNA_Lsm2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13829">
        <property type="entry name" value="SNRNP CORE PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13829:SF2">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016394">
        <property type="entry name" value="U6_snRNA_Lsm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="11">
        <location>
            <begin position="2"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="96"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR016394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2076474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="110" mass="12774" checksum="666F47EDCCB951D6" modified="2022-05-25" version="1">MLFFSFYKTLVGKEITVELKNDLSITGLLQSVDQFLNIKLKDIKVNEEEKYPYMISVQNCFIRGSVVRYVHLPAGDVDVETLQDHSRLEAREQKKVQQQAQQQAQQAQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0P8</accession>
    <name>A0A8J4Q0P8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Transcription elongation factor S-II</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_006208</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000276">
        <property type="protein sequence ID" value="KAF2072476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0P8"/>
    <dbReference type="OrthoDB" id="44867at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13749">
        <property type="entry name" value="Zn-ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.30">
        <property type="entry name" value="Transcription elongation factor S-II, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035100">
        <property type="entry name" value="TF_IIS-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003617">
        <property type="entry name" value="TFIIS/CRSP70_N_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003618">
        <property type="entry name" value="TFIIS_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036575">
        <property type="entry name" value="TFIIS_cen_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477:SF0">
        <property type="entry name" value="IP08861P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477">
        <property type="entry name" value="TRANSCRIPTION FACTOR S-II ZINC FINGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07500">
        <property type="entry name" value="TFIIS_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006704">
        <property type="entry name" value="TF_IIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00510">
        <property type="entry name" value="TFS2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00509">
        <property type="entry name" value="TFS2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46942">
        <property type="entry name" value="Elongation factor TFIIS domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51321">
        <property type="entry name" value="TFIIS_CENTRAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00466">
        <property type="entry name" value="ZF_TFIIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="10">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS central" evidence="11">
        <location>
            <begin position="173"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS-type" evidence="9">
        <location>
            <begin position="291"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="105"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="110"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2072476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="36946" checksum="F31FEBAE1B0A332D" modified="2022-05-25" version="1">MESELVEVKKQLDEAILQSDFDSIISCIKSASSFDMTKDLLKKTAIGVSIGKLRTHKDSLISKESKLLVDKWKAIVEKTNSETTTTKTTTTITKVEINVNSKPVKAVAASSTTTTSTTTTIKSENSSPSLSSSTSSTPPTTSSGTPKRKLESSDSSIKKIKVNKPITGPLADLRNKTIALIAESIVEGKDEDVATESTPEEIATEIESELFNIYQGVTKDYKAKIRSIKFNLKSNDQLKLSLLNRELDIEKFCSMDVMEMANKELQKEREKMAKYTTEAAISNTNNEATTDQFKCGKCKQRKCTYRQLQTRSADEPATTFVTCINCNHRWKFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q173</accession>
    <name>A0A8J4Q173_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PA14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000794</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000016">
        <property type="protein sequence ID" value="KAF2077916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q173"/>
    <dbReference type="OrthoDB" id="23090at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051154">
        <property type="entry name" value="Prespore-cell_inducing_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31137:SF4">
        <property type="entry name" value="PA14 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31137">
        <property type="entry name" value="PROTEIN PSIB-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035230640" description="PA14 domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="422"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="422"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="539"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="651" mass="71089" checksum="E6AC2E41154A2465" modified="2022-05-25" version="1">MKRLKLSFLLFLILVIGNVISQYPADLVLFDTTPDRNPDFALPFTWSDDYWSTSCLQDYLFYSQFPGDYPAYAHGDQSCLDENGNLQVLIINETTWNDRWGVDDQNAVVKEYVPFEFYQYTPLEYYYSNDRFNIFNGLGFDNKALYPLEPVQQNNHHWCMRLNFRVQPVRFEPSFINVRASDDIWVFLDGILQYPGFTGVFREKEIFVDLNRIPPYAIGHVSIFHCNRVVRDGSDGGEDFNLFEIKIRGEINCDYIDMCGVCNGKDDTCCLDGITCFPEEYRCVNRFCIGQDIFDCIEQPYDCNNTMGAQDQCHYPQCIDGAGCQQVPIVCEDGDPCTNDGCDPNFGCSYIPIDNCVGCGVDGCVTTDLCFPRGCDVNNPTVCIDLPEINCTVDDPCVQGYCHLGICEVYYIPNCVPPASSSSSESSASSSQSSSQSSSQSSSQSSSQSSSQSSSESSDSSSQSSSSSQSSSSSQSASSSESSSSSSSPSESSTSESSSETPTTSVTTTPVTTTGVTTTGATTSPATSTTIGGSGSGGTRPPITTTSSTTGNQTSTTTSTTIGQISTTGEVTGSTTSFYHPCDFHRCERGTKCIEFEKQAICVDKSCLDCYSIACPGMGLKCAMIEAKPKSQNNKKSKDDEICCDYIPTCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1Y8</accession>
    <name>A0A8J4Q1Y8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation initiation factor 3 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001431</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000034">
        <property type="protein sequence ID" value="KAF2077242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1Y8"/>
    <dbReference type="OrthoDB" id="21573at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.10">
        <property type="entry name" value="Translation initiation factor 3 (IF-3), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.80">
        <property type="entry name" value="Translation initiation factor 3 (IF-3), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036788">
        <property type="entry name" value="T_IF-3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036787">
        <property type="entry name" value="T_IF-3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019814">
        <property type="entry name" value="Translation_initiation_fac_3_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05198">
        <property type="entry name" value="IF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55200">
        <property type="entry name" value="Translation initiation factor IF3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54364">
        <property type="entry name" value="Translation initiation factor IF3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation initiation factor 3 N-terminal" evidence="1">
        <location>
            <begin position="277"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="497" mass="57667" checksum="B5E23DCB32AB21D6" modified="2022-05-25" version="1">MFKLLSLSRGCKPLLVLNNSHSGLFQLQSSILTTANRFYSKKTSNRDTSAGTDTKRRIPGKNKYDIVEGWDDPINEILDQNFHISQKEPPKSKRMVNNQKKKRVSAFEVFLEEKDKSSPKRVGISQHEKEVEEQDDSIIDENTPQGKLKKMMRDKVAAASKGKSFEPMVPESYYERDLLEEFDEDDYYDNEIGNDMDDDELHQDFMRKTELMEERDIIARLSKERQRDDVRASNQVEYITNQIFSTPTNLPELHSRLSDIDEYKGESTAFNDDALDLDEIRIIDQKGKQMGIYDGKTAFKIAKENNIDLILNVPKNQNAMVIGRLQSLEDYCEIQELKIQRELDVPEMKVAKLKKYILSSNIGQHDKIVKFNAIKKALDTFHPVEVIITFKTEDFDLSEGEKMLEECKGYVRHVGRSKSSETSSRMLSCLFNPLTVQEKEAYEKKMEADRQRELKKQEKLAKKEQLMATVQLEAFEHLDGEEFIENDKKSKKNKTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2M6</accession>
    <name>A0A8J4Q2M6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Follistatin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001802</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000045">
        <property type="protein sequence ID" value="KAF2076899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="7250310at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031160">
        <property type="term" value="C:spore wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007643">
        <property type="entry name" value="Dict_spore_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003645">
        <property type="entry name" value="Fol_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04562">
        <property type="entry name" value="Dicty_spore_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00274">
        <property type="entry name" value="FOLN"/>
        <property type="match status" value="19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035261153" description="Follistatin-like domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="1367"/>
        </location>
    </feature>
    <feature type="domain" description="Follistatin-like" evidence="3">
        <location>
            <begin position="152"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Follistatin-like" evidence="3">
        <location>
            <begin position="867"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="domain" description="Follistatin-like" evidence="3">
        <location>
            <begin position="903"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="domain" description="Follistatin-like" evidence="3">
        <location>
            <begin position="931"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="domain" description="Follistatin-like" evidence="3">
        <location>
            <begin position="958"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="domain" description="Follistatin-like" evidence="3">
        <location>
            <begin position="986"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="domain" description="Follistatin-like" evidence="3">
        <location>
            <begin position="1013"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="domain" description="Follistatin-like" evidence="3">
        <location>
            <begin position="1039"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="domain" description="Follistatin-like" evidence="3">
        <location>
            <begin position="1066"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="domain" description="Follistatin-like" evidence="3">
        <location>
            <begin position="1092"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="domain" description="Follistatin-like" evidence="3">
        <location>
            <begin position="1118"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="domain" description="Follistatin-like" evidence="3">
        <location>
            <begin position="1145"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="domain" description="Follistatin-like" evidence="3">
        <location>
            <begin position="1172"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="domain" description="Follistatin-like" evidence="3">
        <location>
            <begin position="1198"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="domain" description="Follistatin-like" evidence="3">
        <location>
            <begin position="1227"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="domain" description="Follistatin-like" evidence="3">
        <location>
            <begin position="1258"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="domain" description="Follistatin-like" evidence="3">
        <location>
            <begin position="1286"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="domain" description="Follistatin-like" evidence="3">
        <location>
            <begin position="1318"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="domain" description="Follistatin-like" evidence="3">
        <location>
            <begin position="1346"/>
            <end position="1367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="234"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="306"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="329"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="493"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="548"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="604"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="656"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="672"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="707"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="766"/>
            <end position="789"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2076899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1367" mass="144722" checksum="C5B7F7CD535E6189" modified="2022-05-25" version="1">MKYQSIIVLLLCCMVLCSTLADAKKHPNDKDQDKKNKNHHDSKNKHDNNNKHDNKNDKHHKDKNHLNIDLKNCSSFTTHSECDKHSNRCFSSKATACCDKDTFFCLPIPKQVCNNLPPSEWCVAKKDTMMFFNSSCLPEDKKFQFFKPNQLKCKYVHCPHGFTCVDKPARQDLSKYCKSDNPCCHFKSQCVPKSSMLKEFITFEAPKLKKEKKEESHTKIPVIVTPEPTPTPTQAPTQAPTQTPTQAPIVIRDPPMLSTTPISKVVEIENQQEKHHKNKHSKEPKGSKKPKEKDTPEPSIEPTPTPEIIIPKPTTIEPTKTPTEPPTEAPTELPTEAPTEAPTDVPTEVPTEAPTEAPTEAPTESPTEVLTEAPTEAPTENPTDEPISKDENDPFSINFSPSSKFLLEDDSFKSSGPRFTIEPSTFTSQPSKLQQFGNPEDDITTTGQEISSKEEQESTTGQSETSPAFLIDQDILTIEPKVSTTGPDVTIEPSTFSSTTGKSTTTGKATTTKSTTTGKATTTSKPTTTGKATSTTGPDVTIEPSTFSSTTGKSTTTTTGKATTTTGKATTGKATTTTGKATTGKSTAGKPTSTTGPDVTIEPSTLSSTTGKSTSTGKATATTGKATTTTGKATTSPKVTTKPTTTTGKATPKPTIKPESKIRSLDSEEETSTTGKPITTTKPKTTTGKATTGKPRSTTGEDVTIEPSTPTTTRKATTSKPSTSSTTGKATTTKSPTTTGKATTTTGKSTTGKATSTTGEDVTIEPSTSTTGKPTITKKPLPGKTTTTGREVTIEPSTFSASPVNAKDKPIATTSSTTGGSAPKPAPAKGKSFTARATGPIRTLLEVDQEQEEQEEAGFLPLTVGIKCGNLFCNPGETCKVEYNNGYRECSCIPPQTNPPVLSCATVLCPPGKVCIEGFAGPTCVFKPVTTCATVRCAAGYKCVDDQLNGAQCVPIQTCATVRCKAGYKCIDDANGNGPQCVPIQTCATVRCKAGYKCVNDETNGPQCVSMQTCATVRCKAGYKCVDGVNGPQCNLLPTCSLVDCRSGFKCVDDPIDGAKCIAIPTCATVRCKSGFKCVDDINGPQCVPVPTCSTVRCKEGFKCINDPNGAQCIPQASCATTLCKNGYTCIDNNDQGPQCVPILTCSTVECRANFTCVDNSKTGAECVPVPTCATTLCKNGYICIDSDTGSNCVPVPTCAATSCPVGYSCVEDAVEGAKCYIPQVTTCLTARCAPGYVCIDDPVNGASCVKDAPLVTTCLTVKCSAGYVCKDDPIRGASCVKDIPTCASILCPFGNYCVDDAIKGPKCVKTTYNPSNLCATILCPKNTNCVQKGHEVFCEKTNQITCSNIRCASGSCTETINGPICA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3I9</accession>
    <name>A0A8J4Q3I9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000006</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000001">
        <property type="protein sequence ID" value="KAF2078635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3I9"/>
    <dbReference type="OrthoDB" id="20864at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="181" mass="20798" checksum="CBD3F62BFC1D94C6" modified="2022-05-25" version="1">MSKKDKSFGGGTLQFTKVVPKFLQNLKKTTEVDINEKFKDYMNQEDNDEITQDQLEKEAIERALKEEQQLESVGQEKEKLEQEEKEKQLQEQKQEILRQDAIEEEKARLEGRERKIVFQKPSSLSITKQPNAAKNSKDSLNDTIKSIKKTNSKSPKSIEKSNSAKKKQTGGSSMLSFDIDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q8E9</accession>
    <name>A0A8J4Q8E9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002046</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000053">
        <property type="protein sequence ID" value="KAF2076676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="99"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="109"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1369" mass="155687" checksum="D52AD9E4C5164012" modified="2022-05-25" version="1">MDKNNKLLKWSFPALDRDIDSKYTAFYRKGILYPSILPTPCFIKALAIYIDVHKHEKNEEGFYKLPSLQILHQIDSLEIHFLQKSTTKSYCNKKRKRYDDYSNYGSNNDSDDSGDSDDNDDDDSDDDSDDSSDSDDSDDSDDDSDDSDESDDDSDDDSSDESDYNKSTNNGYSLEREYIPPNVKNLYIYIHNSRGDEVYLKGDYIPSTITHLSIQKLSSLNQRFIPKSIKSLEIRYLDRKVCDWKKELKETIQELYIKETSVQVDLKGLDKQKYLELSTETIPTKHSSAKALKLGYRINSDEIYSYKQGVLEAASKDGLYVYQQSSHRIPSSTKHLFFYSKHSFPTIPLGVTTLCTGFNKPLPQGLIPDSVTVIKFTQLNICLSHIHLPRALKYLSLSNYNQAIYGNKHIPQSTTHLELSIKSTNIETNRISMLGFIPPSIRFLKIYFQSTPPDKILVPSSVDYLDMHYIHYDRLVIVDQQVYYSGVPEFKTQTTMVNKLVHLDLGRLYNQPIQPNSLPNTLKKLCFGKGYGQLIGANVIPSSVEELYLGQQSIELDSGAIPTTITSLQISDQSCLASLAQLTKLEKLTLDSECDYSKLSPTIKELVIRNFGSTKFAAPLPMLEKLELGIKDTTFKQVSPDLVKLKELRLLGKDNIIDSKTCIPKSVERLSVPFNDIIFSSLRKKNEPPVNADTEQNDRLFSSSKITSNSDLFFIVWRNRYLKKQIMDIVVDYKRSIIDFSDQENEISQYIDSDLILSNLTIRVSSLESIQYESKIVDQRYRLELLDEMAIDGICILEKNSDLSSLLSMDYITQLACHFNPKYKIPNWIQHLNLLSWRGIKKGDIPSSVTHLKLIGNTKMNCDYSFITSQVIPPSVTRLEIRVNEEFGHLITPNVEVVKIHGEIPSTLPSCFPPTLKRIVLDDITALARNYKFDRDILPFLCGNYETYSKMLSSSSSGTQNISHLIMDKNISISKGTVPNGVRVLVLPDLFNKQIEHLPSSVTEIYFGNHFNQSLSAVYLPPNLLHLHLGYYFSQPISIDDIPYGLTHLSFVVLSKKTSQTLYSGLPSSVCHLTIDETDSECRPKFPEFVKHIKLINHSPIRVPEKSTSVTCYSDSIFLESLFHDEDYEYDQVVDHKIELNQSIKLDLVSNLFNQFIKSNSLMGNIRSITLNYMFKQPLFPGSIPNTVEFLSLEHSEAIGKDAFVGLESLETLILKNVIKLELDPDTLFPESLENLHLDRGFTHDLTKYTFPSKLKNLVLSAGFYHDQLFQWIPKSVSELELGTPLENYLTSHYIDRSIVPVERVPCNITSLILNETVTIQSPSLVPATIRKLKLCGCISYDSFLPTTIESLTLGSSFNSPLNTILQFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4QAX4</accession>
    <name>A0A8J4QAX4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000441</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000008">
        <property type="protein sequence ID" value="KAF2078251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4QAX4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052464">
        <property type="entry name" value="Synovial_Prolif_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23424">
        <property type="entry name" value="SERUM AMYLOID A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23424:SF29">
        <property type="entry name" value="SERUM AMYLOID A PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2078251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="368" mass="43368" checksum="8505D9FA56BFCCB1" modified="2022-05-25" version="1">MKYYSFHKSTIIQFIQKFINGDKQVVSEAYTSGELIWDLSSNESYLECLLENNIIDICLFLIKREIDNNNNNNNNSKDERIIEISFGILSNIFSISKSLCIEYHSNQDLVESILHQIYESKSSTSLSECFRLLHTIVYRKECVSFWLSISLDNDNQLLKRSLYLLDISLFDNLILNLIKYINIISFYSDEILKKDLIKYEITRIITDQLTRNDNYQDKFECILEIIVNLIDQEWCFNLKLYEAIYTVFQNYEHNTTILSLVISILSLSLNNDSINLFFKDNYNFKIINENTTMNNLNDSLDDILQILEYYLNNSNGGLESNKSEITLFLNDQLIKVKEKKEIEKDNDNDDQDKEIKIKYLLNKLNDID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPI2</accession>
    <name>A0A8J4UPI2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nuclear transcription factor Y subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_009392</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NFYA/HAP2 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000697">
        <property type="protein sequence ID" value="KAF2069286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UPI2"/>
    <dbReference type="OrthoDB" id="1097733at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001289">
        <property type="entry name" value="NFYA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12632">
        <property type="entry name" value="TRANSCRIPTION FACTOR NF-Y ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02045">
        <property type="entry name" value="CBFB_NFYA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00616">
        <property type="entry name" value="CCAATSUBUNTB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00521">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51152">
        <property type="entry name" value="NFYA_HAP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="121"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="170"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="297"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="226"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="170"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2069286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="323" mass="37000" checksum="C2E5CFEA9D08576F" modified="2022-05-25" version="1">MASQFSPVILTPDRMNNIGEHPNFGPMVLNPHPYTTVQHHNNNSHHHNHNSSSPDMLINEYPIAYDDRRINDVYRVHNLTHHTINPTQNPNFIFFQKSIEEDIPLYVNAKQYQRIIKRRESRAKFESENRLPKSRKPYQHESRHQHAIKRLRGSGGRFLTTKEKNELENAAAATAANSPNSSANSSANSSTNNSTNNSPSTTTLNTPKAKKNDKLPIQQHINQQHINQQQQNIAQHQQQNINQQNNNTILQQQNINQQMLQQQSPMNQYYQQQKENEQMNGGDASNHIKNLMNNSPNIIGGQTTSHHQLPPLNSSAPFKLSNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UR35</accession>
    <name>A0A8J4UR35_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007123</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000363">
        <property type="protein sequence ID" value="KAF2071551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UR35"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="105" mass="11989" checksum="8502C718EAC26F64" modified="2022-05-25" version="1">MESNEYSFSMNQRNIDEYFQLYQENVARAIPANQSKPKKKKESTLRFFFNQVLRGIFSASISAFFNAFIQSFQPMIGGTQPAETQYLGNDIANDINSAIPNSFDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UR75</accession>
    <name>A0A8J4UR75_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006955</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000344">
        <property type="protein sequence ID" value="KAF2071731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UR75"/>
    <dbReference type="OrthoDB" id="20441at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="75"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="134"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="134"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="264" mass="29839" checksum="27F65EB70739A7B2" modified="2022-05-25" version="1">MESPIRTSYNLPPLPSSSSSSDASELRAIIEKLEKRNAFLEKELDYVKSELQSTRRILTERDELLLHLQFDEEDDGVDLNGQKKKKSRDRVQTEERHKIQRLRSQMKIMENAIEEKDGNILKLSRELIDSKSQFQQHLEMSTNRPNAPIREMPQRSKTSWGFKSSPSASPMSYKSSSSASPVQSEPRFNGAAPSPLNSPVPHSPNFASSSNYASSSSTSMMEFDSPKKMQKKPSIFFKFKGGSKQNSPVIQETNWASQSQTPAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4USN2</accession>
    <name>A0A8J4USN2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_004874</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the histone H2A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000181">
        <property type="protein sequence ID" value="KAF2073816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4USN2"/>
    <dbReference type="OrthoDB" id="9421954at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000093">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="4">
        <location>
            <begin position="40"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="5">
        <location>
            <begin position="126"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="28"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="36"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2073816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="170" mass="18640" checksum="6A48CAFF6C3725E4" modified="2022-05-25" version="1">MSESAAKIESDKKARAAATLAKKEEKAAINKAAAQKRAEAKKNSDKKPESRSARAHITFPVSRVDRLLRDGRYAPRVESTAPVYLAAVLEYLVFEILELAHNICSQNKKTRITPQHINWAVANDLELNQLFQHVTIASGGVLPINTASTEKKASKKTKKPASESAASQTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UT27</accession>
    <name>A0A8J4UT27_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_004347</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000152">
        <property type="protein sequence ID" value="KAF2074326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UT27"/>
    <dbReference type="OrthoDB" id="5970at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="19"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="107"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="183"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="194"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="281"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="298"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="364"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="376"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2074326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="446" mass="51730" checksum="56C245A56F9815A9" modified="2022-05-25" version="1">MDSNNNNNNNNSNSQHGGKTIFVGNIPFNTNYSEIQDIFLPFCKVEKIEIKSGYAFIFLAKGEEEGGQKAIDSLHKSDFKSRNITVQFAKGENKNIQKNPKEIEPCSSIFIVNYNVSKTTLHNIEQLCEPFGTIKRIQMKKNFCFVQYENTSQSKLALETLNGKNIEGHVITVEYVASNLNEANDSNDDRLKYRNNNNHGNNRNSNNNYNNNNNNNNNNRMNNHNNNNRRANGNGNRFNNNNNSNNNYQNHHNGSNSIDRRRNRYDNRSRSRSRSRDRNRNNNNHSNNNNNNNGSNHHEKRNRSSSRSRSRSRDRNREKRNYKRKERSRERERSLSNHSSGDDSSCSDKSRSRSNSRSNDKKQYKGGHHRHHHSNNNKTNNMKYKNNNRSPSRSSNSSNDDDNDNGSRSRSRSRSKNNTKQFNDKYSSRKKSKIISDGADQINISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UT75</accession>
    <name>A0A8J4UT75_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TRAF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_004163</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probable adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000142">
        <property type="protein sequence ID" value="KAF2074536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UT75"/>
    <dbReference type="OrthoDB" id="9049620at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001293">
        <property type="entry name" value="Znf_TRAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131">
        <property type="entry name" value="TNF RECEPTOR ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131:SF94">
        <property type="entry name" value="TNF RECEPTOR-ASSOCIATED FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02176">
        <property type="entry name" value="zf-TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50145">
        <property type="entry name" value="ZF_TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TRAF-type" evidence="9">
        <location>
            <begin position="203"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TRAF-type" evidence="8">
        <location>
            <begin position="203"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2074536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="402" mass="46388" checksum="CF86FAF39148A941" modified="2022-05-25" version="1">MEYVDIDISNFKIESSFVGDISCSNRIENVRTYYNKDPTSLEQDLKLDNIILDEDFECVICKMVVTKQYSCHNGHNHCFDCWSKSLDIDGVCPQCKQPVKKDSLVRNRFYEASLFKLHVNCPLSLQYSIDGGFSHAIKECDKMTLSELMDKHIYKCQYRKVKCVCDQEIYFINLEQHYLQCPKTIFICEHCKSEYNREDNDEHLSSCPDMKVPCSNGCNVDVKRSELLYHLSQDCNKTMISCPLKDVGCQDSFQRDELCSHLKLYNSHLGFILKVAPKVHLLSNDSALYNELRQVQSDYDNIVFATDIMLSEHGAIDIVCHQIKANFIYHAKMCCLTLSLQSKTLGPDVNPSDLECLISFYDKDHQLKEETALPLNTQSQTFSKRYQFSFINIKVLKISSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UTS2</accession>
    <name>A0A8J4UTS2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010515</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001165">
        <property type="protein sequence ID" value="KAF2068158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UTS2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="92" mass="10371" checksum="A0C6C6E9BF2CB67E" modified="2022-05-25" version="1" fragment="single">MDGRSKDMLCKNSFTKVDTSVSMYKVDFEYLDGCDSAYSNKPLCLNTKSNKKGSSIFDTESYLTRPDQCSCLFSGLVLETYVQDCDIMDVTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UU77</accession>
    <name>A0A8J4UU77_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Maltose/galactoside acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003556</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000113">
        <property type="protein sequence ID" value="KAF2075121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UU77"/>
    <dbReference type="OrthoDB" id="27035at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03357">
        <property type="entry name" value="LbH_MAT_GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.10.10:FF:000008">
        <property type="entry name" value="Maltose O-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051159">
        <property type="entry name" value="Hexapeptide_acetyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024688">
        <property type="entry name" value="Mac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23416:SF23">
        <property type="entry name" value="ACETYLTRANSFERASE C18B11.09C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23416">
        <property type="entry name" value="SIALIC ACID SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12464">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01266">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Maltose/galactoside acetyltransferase" evidence="3">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="191" mass="20616" checksum="2F69902C58B2924B" modified="2022-05-25" version="1">MEPINKTEREKMVAGELYLAADPELVAGRKRAKLELSKVNGIVDDDTRNAGYKALLGTHGKQVHIESDFKFDYGYNIHLGENFYANYDCTFLDICPITIGENCMCAPGVQIYTAGHPIDPVERMSGLEFGKPVTIGNTCWIGGSVVICPGVTLGNNVVVGAGSVVTKSFPDNVVIAGNPAKIIKTIEPKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UV82</accession>
    <name>A0A8J4UV82_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_002342</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000064">
        <property type="protein sequence ID" value="KAF2076336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UV82"/>
    <dbReference type="OrthoDB" id="1933281at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034657">
        <property type="term" value="C:GID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16652">
        <property type="entry name" value="dRING_Rmd5p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000143">
        <property type="entry name" value="Regulator of gluconeogenesis Rmd5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013144">
        <property type="entry name" value="CRA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045098">
        <property type="entry name" value="Fyv10_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037683">
        <property type="entry name" value="Rmd5_dRing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044063">
        <property type="entry name" value="ZF_RING_GID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12170:SF3">
        <property type="entry name" value="GH10162P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12170">
        <property type="entry name" value="MACROPHAGE ERYTHROBLAST ATTACHER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00757">
        <property type="entry name" value="CRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51867">
        <property type="entry name" value="ZF_RING_GID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CTLH" evidence="9">
        <location>
            <begin position="173"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="RING-Gid-type" evidence="10">
        <location>
            <begin position="348"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="348"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="RING-Gid-type" evidence="7">
        <location>
            <begin position="348"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2076336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="405" mass="45785" checksum="120D0455749FE2DB" modified="2022-05-25" version="1">MESSSGTTSISSSSSKIDFDSLDEGLTRIAKKQKTIDTKAISDINNILVQLNTCKKTISLHSNDMETDDLIPKAITKLNQTLIESQLCTKVTNDHRETHAPISKFGKLLEKSFRSDIEKSTKDLEFDKPTLNEIIINHLYRAGRFDLGDIFVEESGFDAEEAKRTKDLFIDQQRIVGSIESYNLDSAIKWSKENRKGLKDINSSLEFKLHRMQFLYLLSMGKQTEALQYARHNFSEFSSTNQKDIQHLMGAFIYANRLETSPYASIFQMDSIENAWREVKSIFTKDSSTLMGVAQESPLSITITVGVRALPTFLKLSSFAMLKGANDDSLTVEISVDENYKFHSIFACPVSREQSTKINPPVMLQCGHLLCKNSMQRLLKSASTRFKCPYCPTEQSLNNVKTVNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UW24</accession>
    <name>A0A8J4UW24_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Myb domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_009414</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CEF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000702">
        <property type="protein sequence ID" value="KAF2069270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UW24"/>
    <dbReference type="OrthoDB" id="1410009at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11659">
        <property type="entry name" value="SANT_CDC5_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000021">
        <property type="entry name" value="CDC5 cell division cycle 5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047242">
        <property type="entry name" value="CDC5L/Cef1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021786">
        <property type="entry name" value="Cdc5p/Cef1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047240">
        <property type="entry name" value="SANT_CDC5L_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45885">
        <property type="entry name" value="CELL DIVISION CYCLE 5-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45885:SF1">
        <property type="entry name" value="CELL DIVISION CYCLE 5-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11831">
        <property type="entry name" value="Myb_Cef"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Myb-like" evidence="10">
        <location>
            <begin position="2"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="11">
        <location>
            <begin position="2"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="11">
        <location>
            <begin position="54"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="10">
        <location>
            <begin position="54"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="108"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="376"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="737"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="121"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="386"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="445"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2069270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="769" mass="87547" checksum="582CD807A2E11633" modified="2022-05-25" version="1">MRPNVKGGVWRNTEDEILKLAVMKYGPHQWARISSLLTRKSPAQCKARWFEWLDPSIKKTEWSKEEEEKLLHLAKIFPSQWKTIAPLVGRTAAQCLEHYNRLLDEVQRQQSGSGADAAGGEDPRRLRFGEVEPIPETKPAKPDPIDMDEEEKETLSEAKARLSNTMGKKEKRKFREKQLEEARRLAALQKKRELKAAGIHYNPKTKNAKDFRDFDISKEIPFFQKPKAGFYEVPEEELRDDPNRDLAFIGKRLDQIENPNYLQRQDKLNKIEDIKRKKKEMMSLPDSIFDLSKKNDVDQTLKRSKLSLPSPQLTEQDIQEIAEYEKANDVTGGQQGVTNALVGKFPVPANRTVARTPLREDVIMMEAQNLLNLSNAETPLKGGNNPSVTPLPVRSSVPTSSSQSSASATGATPNPLAVHLTPARKQQQQQLDEYSREDKFKKQQNKNQLLSKLKTLPSPSQSYKLALPDEPMDDMDASASDATELDQSEHHIREQQQLKHKEQFRLRTRSTVLKRNLPRSQNYLHIAQGSDSIHIDETSSVPIQTQIQNILASEINRIILHDNLTFPLALDARAQDDASYQHFSNTELEHADHLLRLEMAQMASEQPLDAHALLSQMDAMRAQYIYLPSQAKHLAISSLTPTQLIDALAHQHTDLIHQIKSSSSKSVHLEKKLNIYNGGYHNRCSALLKDIDSLYSAYEKANIELECFDQLAATESVHMDKRLADIQNQVYDQCEIESRLQAKYAALKNELSSLKSTNNNHNHNNHHNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWQ5</accession>
    <name>A0A8J4UWQ5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">H15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_000897</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the histone H1/H5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000019">
        <property type="protein sequence ID" value="KAF2077776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWQ5"/>
    <dbReference type="OrthoDB" id="1110759at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031492">
        <property type="term" value="F:nucleosomal DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045910">
        <property type="term" value="P:negative regulation of DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00073">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005819">
        <property type="entry name" value="H1/H5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005818">
        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11467:SF36">
        <property type="entry name" value="HISTONE 24-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11467">
        <property type="entry name" value="HISTONE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
        <property type="entry name" value="Linker_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00624">
        <property type="entry name" value="HISTONEH5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00526">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51504">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="H15" evidence="8">
        <location>
            <begin position="21"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="96"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="123"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="148"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2077776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="185" mass="19645" checksum="19641EA1DD3683AE" modified="2022-05-25" version="1">MAPPKKSTTKKTTAAAKTKGSHPTYQVMISAAIVALRERTGSSLVAICKYMESNYTLQPEFKEEVKKALKRLLTKGTLERVLASYKLTEAGRAEYKKLIGSSSSTTTAKKPAAKKAPAAAESDSSEEEEEEEAAPEPPKKKTAPPKKPAAAPAKKKPAAAPKKPVAKSAKKNAAKKPAAKKTGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXM8</accession>
    <name>A0A8J4UXM8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007559</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000410">
        <property type="protein sequence ID" value="KAF2071130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="21610at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="235"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="296"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="336"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="362"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="378"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="390"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="413"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="444"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="563"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="596"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="658"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="678"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="690"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="714"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="809"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="878"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="954"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="983"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="999"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1010"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1046"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1056"/>
            <end position="1109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1462" mass="156845" checksum="A18236415911C58D" modified="2022-05-25" version="1">MISNLANVVHCVKSIDSISKDSTMIHIDLFLENLDGQNTSKMVDKLYNDIVMIDNQVKSLELKSLKYPVNVPTDFENPQLQQQHKLIGENDIHQHIRPRHFKSDINFQDDWVDWNSEDDIDFEKELSPKSRKNTHQRKLQALEPENDIDDLYNQNFDNLDTNFFDFEDDDLISKKNILNFDQDSFISDHSSSNEKDETNESKEFSSSNNQQSSSTESNKKQSSFSSGDKSLENQSSSNTKSSEASSNEKSPTSNEKSPTSNEKSSSEKESSSNEKSSNEKQSSSNDKSSNEKESSTKSSSEKQSSSSDMSSNDKSSSTIENSSSEKSSREKETSSSEKQSSSSEKQSSSNEKSSSEKEFSSKESSSSEKQSSSTIEKSSSEKSSSEKETSSSSEKQSSSSEKKSSSHNESSIDRSSSSEKQSSSNEKSSSEKQSSLNENRKSSSSDQVSPKSSSSYSSSDKSSDIESSSKSSSVEIQSSSSNAKSSSDSGSLSKPSSSESQPSSPSPISHSSSNEKSSGENSSSNEPSNSKSSGEQQSSNGSKEKSSSNNSSSSDKSTESDDGTLSSSSGKSSMENSSSDQKSSSSDIEASGHKQSSSSNGEASSNVKSSSDSGSLSKSSSSESQPSSPSPITQSSDSNEKVSSSNDKSSSDNDKKSSSSSTKASGSNENSSTGNNQSKEKSSSEKESSEQSSSYYSSQSSFWSSSSSEKESSEQSSFDSKQPSTENSSNDNQSLSSQNENSSHQKQSSSSHQQFSSSENQSSSNINENSSIEIASSGDQASNESSNDMSSSSRDNKSSTSEASNVPISSTSTQSSESSTQAQSSSSESPIVTPTSPVSSISSSSEAPTQSSSETPTSAQSSSSSEAPRMAPRMAPKTTPTQSSSEAPTATPTATPTATPTSAAAQSSSEAPTSTQTSSEASVPSSSTESPKEIPSSTPTQSSSSESPKATPTMTQSSSSESPKVTPTSAQSSSESPKVTPTSAQSSSHEVSSTSSESPKMTPTNAPAQSSSESPKEIPSSTPTQSSPSESPKATPTSTPSESPKETPPSTPTPPISSSSESSSSETQQSSSFETTSVSSSSSESSSSEIISISSSESFETPTPSETPTQPIKSPYKCGIEVKQEVIRQITSSIAIWCVTIYNHEPFVHNEIAVLRKEFSLSGGIEFHITNIWETFTPIDEGKAFSLLYIGKLTPDEFIIICDDEYKDTLPPTPTKPVPTPTPTKPKPIPIKPCTVSYSQKRIGYVRVGTLVQYIWWVEIANDGTNNINHLLLKFSDSVQHVNGLDPVQFSNDKTTYYEIPVNSLPLRPHSFYSFTYFSVGTSQVSLDFPEIKECSRSNRPPNSFITNIENPCSSSVEQESIDTDTDQDGNVITKWAVRIQNTSPSNNLYDIAFELKDAVQSTKGVEYGTLNENTPIFHLPTQLRSLGIQKSQTYVWYYIKKGTSPAHISVATRLCTPEYYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXR1</accession>
    <name>A0A8J4UXR1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Potassium channel tetramerisation-type BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007384</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000391">
        <property type="protein sequence ID" value="KAF2071295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXR1"/>
    <dbReference type="OrthoDB" id="17814at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Potassium channel tetramerisation-type BTB" evidence="1">
        <location>
            <begin position="63"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="111" mass="12562" checksum="92AD1E36A675451C" modified="2022-05-25" version="1">MFNLQLFKTSRNPTLGMKTTLLIILINGHFGSLQQDITELLKRKDVLLDVTKVIDVSVIPDPITLNIGGFKHQTTKATLTKIPNSFFDLMLSGEIDIKPMTNKPNTYFIER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UY16</accession>
    <name>A0A8J4UY16_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA repair protein XRCC4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_007421</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000395">
        <property type="protein sequence ID" value="KAF2071263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UY16"/>
    <dbReference type="OrthoDB" id="21361at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032807">
        <property type="term" value="C:DNA ligase IV complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005958">
        <property type="term" value="C:DNA-dependent protein kinase-DNA ligase 4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010165">
        <property type="term" value="P:response to X-ray"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.210.10">
        <property type="entry name" value="DNA double-strand break repair and VJ recombination XRCC4, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010585">
        <property type="entry name" value="DNA_repair_prot_XRCC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038051">
        <property type="entry name" value="XRCC4-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053962">
        <property type="entry name" value="XRCC4_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053961">
        <property type="entry name" value="XRCC4_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28559">
        <property type="entry name" value="DNA REPAIR PROTEIN XRCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28559:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN XRCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06632">
        <property type="entry name" value="XRCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21924">
        <property type="entry name" value="XRCC4_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58022">
        <property type="entry name" value="XRCC4, C-terminal oligomerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XRCC4 N-terminal" evidence="6">
        <location>
            <begin position="28"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="XRCC4 coiled-coil" evidence="7">
        <location>
            <begin position="153"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="249"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="317"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="249"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="330"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF21924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2071263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="444" mass="50735" checksum="ACF4E2854D49E6F4" modified="2022-05-25" version="1">MNEVSLTKFENVPLGNNVVSLSTINCLLVKCIWQPTSFSIHITDFNSVWYSNVNTKYIETVLKPPGMSIDQYMKLIKESLIKQDIEKKQFNYKIEKSKSASIDIEFQIFIKLQEYDQINIKSPIQLIRSNSNDNSNVSGNTKGIATTQQFFEWVFDRYKYLEDQNLQLKTQNIDKQTQFDQLLDQNHLFIKEKDNLENELFNKFVIILNEKKTKIKEYKSKYLNLLTSVESKKNNDSVNDENKSCCKCNQNNSSIDNSSQNYEGSQEYMTPKKTRKRITPTPSSANENKMTFDEHSMTLGYIDDVDDYFKVPTTVRKKYKPNSDSSNNGSTTPPISTATATKTTTTTTTNTTTKNPTLPIHGKKKSPLKATPHSNHQYHQSPKKQLISNLISKTASTNHNYFPSMKPATPLKPKHKGLFGGYNDDDDDQDVEEDIGASALLNNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UY18</accession>
    <name>A0A8J4UY18_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ATP synthase subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">CYY_007401</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000393">
        <property type="protein sequence ID" value="KAF2071278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UY18"/>
    <dbReference type="OrthoDB" id="30023at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18113">
        <property type="entry name" value="ATP-synt_F1_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18116">
        <property type="entry name" value="ATP-synt_F1_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01132">
        <property type="entry name" value="F1-ATPase_alpha_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000002">
        <property type="entry name" value="ATP synthase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.150.20">
        <property type="entry name" value="ATP synthase alpha/beta chain, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01346">
        <property type="entry name" value="ATP_synth_alpha_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000793">
        <property type="entry name" value="ATP_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038376">
        <property type="entry name" value="ATP_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033732">
        <property type="entry name" value="ATP_synth_F1_a_nt-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005294">
        <property type="entry name" value="ATP_synth_F1_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00962">
        <property type="entry name" value="atpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009884">
        <property type="entry name" value="PRK13343.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48082">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48082:SF2">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00306">
        <property type="entry name" value="ATP-synt_ab_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 15" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 15" id="KW-0139">CF(1)</keyword>
    <keyword evidence="6 14" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="9 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit N-terminal" evidence="18">
        <location>
            <begin position="82"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="16">
        <location>
            <begin position="209"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="ATP synthase alpha subunit C-terminal" evidence="17">
        <location>
            <begin position="432"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF2071278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="562" mass="62601" checksum="06588C5808B06038" modified="2022-05-25" version="1">MNVAPKTYLTSYWETLKTTIKELNNTTNVALNADVKKNEKFLYESVMKFTCHQEEIYNYFVLNNFMEGLKKKYEILNTSPNYGKVLSIKDGVVKVSGLREVKIGEKVEFIGKKLTGMALNLEADYVGIVIFGQDTVLMEGDIVKRCEENFAITIDASMLGRAVNVLGEPIDGNGELKFDFKKTTRIMSVERKAPGIVTRKSVHESMLTGVKMVDALLPIGRGQRELIIGDRQTGKSSIAIDAIINQKVTKDIVCIYVAVGQKKSTVRRLVEVLKTKGALEYSIIVVSTAADPAPLQFLAPYAGCSIGEYFRDQGKHALIIYDDLSKHAVAYRQMSLLLRRPPGREAYPGDVFYIHSRLLERAAKLNAKYGSGSLTAFPIVETQAGDVSAYIPTNIISITDGQIFLEKELFNKGIRPAINVGLSVSRVGSAAQSSVMKRLAGALKLELAQYRELARFEQFSSNNDAVTTKALKKGKLTIEMLKQVNYNPMSLGFEALMIFAMGNTYFQNLNLLNIKDEEKNLIAFIKNKVLFNLYCASIDSINTFNVKDNLFSVMCTSYVNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYH8</accession>
    <name>A0A8J4UYH8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF676 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_005902</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM135 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000248">
        <property type="protein sequence ID" value="KAF2072780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYH8"/>
    <dbReference type="OrthoDB" id="273452at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000407">
        <property type="entry name" value="Protein FAM135A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022122">
        <property type="entry name" value="DUF3657"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF5">
        <property type="entry name" value="DUF676 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12394">
        <property type="entry name" value="DUF3657"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF676" evidence="3">
        <location>
            <begin position="701"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="218"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="268"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="365"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="429"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="659"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="272"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="367"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="447"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="473"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2072780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="977" mass="110934" checksum="13CE94D3654D4BA5" modified="2022-05-25" version="1">MLFGVINVLVYIESLRVIDLLKKGVYNIKIKCYRENDNTVLATPYILRAGTGGTNTRLGETNHNIKDHEILSALNTYSSKKFFVQYVNEFILFGNYFIFQIEIPLHNFYSNNIIIDFDLVFYTTEKEDTVEEKVVSQQKLKIGKPGYGVHQYYPITFSDWYFCLLKTTISTCMIDIVFKHHSIDSNPTIGIQPNSNLYSLSSSTSSLAMLSSSGGSITTSSSLSSSPNGSSNTNSSSMSHPILPTYHSSPNSPILKHRVVSRSSLMSLPIKQQQQQQQPSSPSQHTSLNYSYNDNRVLLDGFFDEFFEKKYPEVISFFVESYYRIGKKYKVLLSLLKQTQLTQLGDIVNIKPLNIPPLDHLMADAAQDSNNNNSKQQQQSPSSLNGSPAISSWKMATTQSGNEGSVLSMKSKRKSFLRSSLDLSSISISSSLSDQQQIPSPHSSSTTREELTEEINHSDKQHKDKDTIDVDASNNSPSSFSSSSSSMFSLSSFSTTSTNTTTTTNNSNSTNNINNHNHQTNGSNFWHSPTKPAAISGKWRKILENFQSLGDQVTRLWSHFAVVYQLVSRESCFKSLISFEKKCIQKWSFRTQKIIHYIQNTEDLLLIDTSPPSSTFLQSNSFFQYKPFKIQTIPIKLDTVYDSQPIFFEHTLKFNNNQNNNYSQFNNKNSDNNSVNNNNVNSNNNSNTKRFMMSITNYPGKHLFVFVHGLSGNSYDLRQFKNYFALHFPHFLYLTCSSIQENTLEDIQEMGEKIAAEVQEYLLDNAMAQITKISFLGHSLGGIVVRSALTSDRLQPYLGKLHTYISLSSPHLGTKFYGGSSLVTPAMWVWQKFTSSTCLKQLMMQDSSTLTDTFIYKLSKTKGLEYFQYVFLISSEQDGYVPYHSARIEIPKELVKDGSIKQNSLTKQMVLNLLEPIQSSENLFRDPSFDRFIRLNVIFEAHKGVDGVIGRSAHIRMLDQTWFMQMFVQLYKSYWEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYT7</accession>
    <name>A0A8J4UYT7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005124</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000196">
        <property type="protein sequence ID" value="KAF2073575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYT7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="136" mass="15538" checksum="14B93F118D7202D8" modified="2022-05-25" version="1">MGDYSEFVFTSNNGTVCLLTSVSKWSIMTSSTLYVGLTPSYEPPVISTIKEKHMILPNHSSVVYTIVQDSPKLELRKPLFCKGSNYNHTSYQHQPFQYIFSYYPYTQYTHNVKLVQLSNSQSFEIQLSYVGPEIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYV9</accession>
    <name>A0A8J4UYV9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PLD phosphodiesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006577</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000310">
        <property type="protein sequence ID" value="KAF2072103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYV9"/>
    <dbReference type="OrthoDB" id="14911at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030572">
        <property type="term" value="F:phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09110">
        <property type="entry name" value="PLDc_CLS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09159">
        <property type="entry name" value="PLDc_ybhO_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21248">
        <property type="entry name" value="CARDIOLIPIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21248:SF22">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="2">
        <location>
            <begin position="154"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="2">
        <location>
            <begin position="517"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="305"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="615" mass="71616" checksum="C1E18D4C5CC4D815" modified="2022-05-25" version="1">MMMKKFNYGSIKEMFKSNQVKLRQNLKDNPVWKKVYTSWQKTTQRLTGLNPYYSRGNTVSILFTGEHAFGSIWETIDSAKESIQIETYTIEADEVGRYTLDLLTKAQSRGIQVKFMTDAIGSMSLSDFETYPLRQKGGEIYSFNPVNITRMEPFSMRNHRKIMVADKKIGFCGGMNLSSKYAGSKITGGVDYFRDTHAKVVGPIVKDFSRVFYDSISRSLKLQETNQEFERLKSEKISSALAAASLEELDDDVFPFDRPIETILAPSRNFDRFNKKHPSYNTSRIQEIDIEDDDDLQQDKERTVNSSNNNNNNNNNNNNRLQKTEEEKEASILEYFEEQINRKKVMRNNDRDVKMDGPLLDKDIYFAEEDEDDQDFIIDESELDTRFDMEEEDNIDEEILDDLHDVEQKKQDEEDGKPAVINNVFVQIQQSNLLSNRREIQKAIVLTLRNAKNHCYFTTPYFLPPIRLRNEIIHTANRGIDVRIITAGLSDVPWFRYASRHIYHKFLSRGVKIYEMQTLTLHSKTMTADGIYSSVGSYNLDTWSQKNLEVNLSFVDPSIAEQMEKQFDKDLLICREITLEEIQNRGWMEKLISYVCYQMSRVLRPSRSKAIFINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZ29</accession>
    <name>A0A8J4UZ29_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cellulase</fullName>
            <ecNumber evidence="3">3.2.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_004710</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 9 (cellulase E) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000172">
        <property type="protein sequence ID" value="KAF2073965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZ29"/>
    <dbReference type="OrthoDB" id="14118at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001701">
        <property type="entry name" value="Glyco_hydro_9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22298">
        <property type="entry name" value="ENDO-1,4-BETA-GLUCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00759">
        <property type="entry name" value="Glyco_hydro_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035261207" description="cellulase" evidence="10">
        <location>
            <begin position="24"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 9" evidence="11">
        <location>
            <begin position="50"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2073965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="488" mass="53774" checksum="FB60703441110196" modified="2022-05-25" version="1">MYIRKSKFILTLLVLVLSCSCNSFTIKHNTKNNDGGDTDGINQFMADTDFCDVLNKALMFYKYNRAGRLPDDDVPWRGNAALGDSSPGSSPDANGDGNLSGGYFDAGDHVKFGLPMSASMTMLGWGYLENKDAIEKCGATELYVQDLMYGWDWLMAAHTGPNEFAAQVGNGEADHAYWGPPEMMTMARPTYMINEQAPGTEVAMESAAALSICFMILNETNPDYANNCMDHAKQLYSFGDNYRAVYSDSVPDAQNFYKSWSGYDDEIVWATLWMHRATGDSSYLTRAQSDYDSLTIGKMAQANSHDWDLKAPGVCLLMTQAFPGTQQYVTDFEGFMNWWLPDGGVPYTPGGLAWIREWAPARYASNAAFLSSVYAKSGADTKYQTFTQSQISYILGNNPNSQSFVVGIGPNHPINPHHRASHHSLTDNIMSPVNNTYLLLGALVGGPSQDDSYKDDRTDYVRNEVACDYQACFVGNLAYLASLASGNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZF5</accession>
    <name>A0A8J4UZF5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005736</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000237">
        <property type="protein sequence ID" value="KAF2072943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZF5"/>
    <dbReference type="OrthoDB" id="10027416at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="569" mass="65762" checksum="1CE586E2692248E7" modified="2022-05-25" version="1">MNNNDQLFFSIFKNKYLMQKIYCFYYDYTVLRNITDLDKKHNHISSLHHQIPIVYHLQTLDHYVIYNSHKHKHLITHFILSTRFYLNHLNVLQQQNNTTTTPIIFGISINDRNDPILFAHQLPPSIKYYRFKLTETVTKNHIPPSVTSIYLESLPIDVGVIPNNVKTLALYISNPSDDSYQINPKWFPQSLTSLTYVDVFAESGVDMSELPKSLTYLNIKNSLTGIGHDSIKYLLNRSYESFTVPSGVTHFHLCGGWDSFEDVVLPPSVTHFEIVKETIDQFIPKIPNFLPLTLKSLSLETCSNSSINIDNCPPNLLYYSNYNERCEKVVPYIPPLAKKVILSTDENTPNLPSIPKSVTDLSIRCSVYDYPFKGTIPNHYFTNNLVKLVFHYFGQLEPGVIPSNITDLELGTHNPIIQGVIPCSVTKLSLLCDHVVQPIIIPQSVTTLSFWDEKQVYSNTLIPNSVKTLKFSFIDFTTLKINYLPKSISTLCIDDTDSYQGSEFEERFQIQFPNYNIDLFPSNIKLVKFTQKGDTIKRCYHFINQKVYMEIDFEKDRFMYNFKKINTII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZU1</accession>
    <name>A0A8J4UZU1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005147</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000198">
        <property type="protein sequence ID" value="KAF2073528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZU1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="206" mass="24586" checksum="E560F763C0998483" modified="2022-05-25" version="1" fragment="single">MDNSSNNNNSNNNRNTATLFRSIFNNVYLRKCIFKYFTPPTDGYLMCYNGKFYQNRDDYNLKQLLESNLDESFILDKLKRFKDLLSDNSNNNINTTTHTYYQDWLDTPEEGVSMDQNMFTTTNKQEFNDLVDELFSRDHLFKKMVAHPDTDLDFNSIEYDKSMGDQELFEKIQNNIRCFKYEMLIRDQSIDDIIKFSERVFRIADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V010</accession>
    <name>A0A8J4V010_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_004917</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="AJWJ01000183">
        <property type="protein sequence ID" value="KAF2073783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V010"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="179"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="11">
        <location>
            <begin position="435"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="20"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="93"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="48"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2073783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="503" mass="55928" checksum="C6F910B605A375C5" modified="2022-05-25" version="1">MLSNNHTVFSSNFINAAADDRDEGGGITIISSNNNSNKSTPSNGSPTKNIPITPTKGIQFTKTPVKKNNTLVSSSSSIQLCLLNHELKVNGQQQQNEEGDLDHNLHFGNGDNQHNSDNEVSDDEDLESFNSESSDTGISSSGGEGNGEKESNDDFTPILGSSLPSGSIKISNNIDLNSFTVTQMVGKGGFGKVHQVIHNSTKKVYALKTIKKNHIIRKKSVSNTLAEKDILKKIDHPFIVKLHYAFQSEKKLYLVMDFVNGGQLFFHLQREAIFSEAQVRFYIAELVLALEHLHEANIVHRDLKPENILLDSQGHCILTDFGLAKEEVIDDSHNSFCGTLEYQAPEMIQGKSYGKAVDWWSVGILMYDMLIGKPPFECKNRATMQEKIANEKPKFPQFVSSSARSLINSLLHKDPKQRLGSKNGAKDIKSHTFFKSLQWRKLEQKEIPPPFVPTTKGVEDISNFDLLSLKECLRDSVHGSSPNLSASQQKYFDGFSYVRSPLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0E4</accession>
    <name>A0A8J4V0E4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000096">
        <property type="protein sequence ID" value="KAF2075540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0E4"/>
    <dbReference type="OrthoDB" id="28875at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="1">
        <location>
            <begin position="204"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="287" mass="32825" checksum="49A53E69CC7C38C1" modified="2022-05-25" version="1">MATRDRTPAFKLIDLEYNNKTKNHSIADPKDRDHGNDIPLGEKEKDSVQLKLVNFVPAWLQRVNDIDSNICKIKLKIERLEQLQENKVLPTFDDDGESIVKMEIKTLKIIRLIDATNQLVKTLGEGFDLSIEDTRMKKDIQRSKIATMQTVSLLFKQNQNNYLTKISLEMALPTWNEIQDIKAGDVPPSISLTNQQMNLVNGIVCDVHARDREIKKVLSSIQDITQLIHDLGLLVGQQDSVLDQIEYNLDHAEGSTIGGDTMYMYYICSFYRNHNDNVNHINKTNES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0J2</accession>
    <name>A0A8J4V0J2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009393</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000697">
        <property type="protein sequence ID" value="KAF2069287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0J2"/>
    <dbReference type="OrthoDB" id="20254at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="144" mass="16055" checksum="9B4DA568B932DC2A" modified="2022-05-25" version="1">MERPKKKTTLRFSEEKEDIIIPEKNEISPDLDAKDEEPETASTTATATATPTTNTKEAMIASIPQDVYDKVKDWKVKFAFITKGTNLMSTSKQHKFKQPAHVDSAIRFLAESGVKDSTLVFENDSSLIFSAGSYDGLDIFDDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V252</accession>
    <name>A0A8J4V252_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002642</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000074">
        <property type="protein sequence ID" value="KAF2076078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V252"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="825" mass="98139" checksum="ECFBE7D285F2C02A" modified="2022-05-25" version="1">MGNNLFLLYIYRNTYIRNNIFKNFNRPNDLFLNLKVKSTYDDMNFKQLVLQNNSFLFKDKIQRFKDLFSTTGNSNSNSDGDKEYYKDWLDFNSNFDNPHDMIKTIIKSLSNEIVDQYFKDIVYFLFKSRVEISQLFQEKIVWTRDRIDFIDQLLSSDPTMYSFCTKELHHKYDSIDTIRWLVDQKRMKVNKYEFIKDPLFLDNKDFYRSITGWSDYAFARAAYYATYKDHSFVHLLKWILLVSPYFNYDLYIQSNPSTMGIDHPLLKKEVEHYPLYGVNVVYRKNDFDIFNIVTDEPFYPESLELFNYLVLVFPSDLRKYLIYYPRIRINDDALADSFLQFNLNKMLIVYTEREDLHFIEKFEGYKLRGDNLFNRKETVEFLAANIPWVLRDSSVLNGGVVDNPLELLTEVFNRDENDGCFISYLFSAPTTLNHLIASQREEDIEQIKGLLKIYYIDQLEFVLDVFSAGHQTLFQYVFDCFWQGKVFCKEDSDVASLDDTDCLDTESRQEIEMEKQKRLQENRVKLVQSLFYRCILLHRDHHFTRLFFNMLPSDEDKKRLLASNVFIVYNSLVTVQNERDRLVALKNIEFVKEMLGLYPIETIAHSDVEIMTQLFDAPLYKTLPKDTHYNCMDLVKFAFTQESVSLARALMSTLTPVDRMIVSESINEMVTSYIYDQGLLKVPLYFIHEFSESFDETFLEKMVNVALCTKNRNLFDILLHHTPIRLVAPRSRDHEQTMLVTTLMNMLKSKYYIPSLKPLITEPLPNIDPDILAKHEFTKTLSDLTACRFKHYPFRNDDYKFYKGLIQKHFNVLKKNKYCDYNFVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2C7</accession>
    <name>A0A8J4V2C7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Alanine racemase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_002560</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000071">
        <property type="protein sequence ID" value="KAF2076153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2C7"/>
    <dbReference type="OrthoDB" id="186866at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008784">
        <property type="term" value="F:alanine racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030632">
        <property type="term" value="P:D-alanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00430">
        <property type="entry name" value="PLPDE_III_AR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.37.10:FF:000006">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.10:FF:000002">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="entry name" value="Lyase, Ornithine Decarboxylase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01201">
        <property type="entry name" value="Ala_racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000821">
        <property type="entry name" value="Ala_racemase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011079">
        <property type="entry name" value="Ala_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020622">
        <property type="entry name" value="Ala_racemase_pyridoxalP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00492">
        <property type="entry name" value="alr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30511">
        <property type="entry name" value="ALANINE RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30511:SF0">
        <property type="entry name" value="ALANINE RACEMASE, CATABOLIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00842">
        <property type="entry name" value="Ala_racemase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00992">
        <property type="entry name" value="ALARACEMASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01005">
        <property type="entry name" value="Ala_racemase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="Alanine racemase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00395">
        <property type="entry name" value="ALANINE_RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alanine racemase C-terminal" evidence="7">
        <location>
            <begin position="336"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600821-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR600821-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2076153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="462" mass="50827" checksum="16CC2BB4345F613B" modified="2022-05-25" version="1">MTIVQNQIKPNVGNKMLKSSSADIILTTSFVKNSSGGTGITSPSNSKSTSPISSPILKHSTSGNNISAGNNTTFLNNNNIFRKTIAWIDREAISSNVRNQLSRMNGTTKLFAVVKANGYGHGAIEVAKAAKRGGATGFCVAIIEEALELRNHDSFKDDPLLVMGLSHPNEAPIMAKKKISITVTNLEWLQDALLFMSQQQKNSQNGASTQPLLPLNVHVAVDTGMGRIGLTSVEDLAKIQSFFNQHSAYFKFEGIFTHFATADSTDSTLYAIQFEKFHRMLSSLAKKPTYVHISNSASALYHNPPIWCNMIRFGIAMYGLNPGGKSIPSPFPLKQALSLVTELVHVKRVKEGDTIGYGATYKAKEGDWIGTLPIGYADGWRRDLQGQTVLIQGERCEIIGRVCMDQCMIRMPREYPVGTQVVLVGKSEKDEITLQELSDKLNTIHYELACCFTNRVPRLYFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V311</accession>
    <name>A0A8J4V311_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Beta-lactamase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009367</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000690">
        <property type="protein sequence ID" value="KAF2069314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V311"/>
    <dbReference type="OrthoDB" id="5946976at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019216">
        <property type="term" value="P:regulation of lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052794">
        <property type="entry name" value="Mito_Ser_Protease_LACTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46520">
        <property type="entry name" value="SERINE BETA-LACTAMASE-LIKE PROTEIN LACTB, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46520:SF1">
        <property type="entry name" value="SERINE BETA-LACTAMASE-LIKE PROTEIN LACTB, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase-related" evidence="1">
        <location>
            <begin position="81"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="441" mass="49679" checksum="D8C94F58880A28AB" modified="2022-05-25" version="1">MNILNTIKTYRGLLTTASFLTLSTGSFLAINQKDKVLCEENKVPTPLNNQQQQYFNASRSKLDPITRSDIAKEIISWKEKCTVPGISVCVMSKNQTVFREAYGYSDIENGVVMNTSSKLRIASISKTLTSVAIGLLMEDNLLKLDDHINKFVPNFPKHPNFPEVEITVGHLASHLSGIRHYLKGEKSEFYSVKQYKSAKEFNPFNHSDPLEIFISNPWIEKISTTKPGALFHYSTFGYTLLGAIIENISGTDYKSFMRTRVFNRCGMYNIFADEHNVIIPNRSKQYTLRPLDKTNIDRMELMNTDFTNSSYKWAGGGWISTAEDICRFGSTLISGKLLKPETINTLFTPLKTTEGVETNYGFGWMNLKNNLIDNDNDNPNILDQDNKIIFHTGHAMGGATVLVLLPKEQIVVCVLANQEKIVGIQDLGFKIARLVSKSEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V324</accession>
    <name>A0A8J4V324_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_009335</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000682">
        <property type="protein sequence ID" value="KAF2069344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6161812at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008782">
        <property type="term" value="F:adenosylhomocysteine nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008930">
        <property type="term" value="F:methylthioadenosine nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019284">
        <property type="term" value="P:L-methionine salvage from S-adenosylmethionine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056681">
        <property type="entry name" value="DUF7779"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46832">
        <property type="entry name" value="5'-METHYLTHIOADENOSINE/S-ADENOSYLHOMOCYSTEINE NUCLEOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46832:SF1">
        <property type="entry name" value="5'-METHYLTHIOADENOSINE_S-ADENOSYLHOMOCYSTEINE NUCLEOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25000">
        <property type="entry name" value="DUF7779"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NB-ARC" evidence="2">
        <location>
            <begin position="427"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7779" evidence="3">
        <location>
            <begin position="645"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="762"/>
            <end position="782"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2069344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2268" mass="263235" checksum="4281886B7B035679" modified="2022-05-25" version="1">MNQHCVCILHSGHTEVFSTISIFKTNSVIKFSDVKQSSNFAYREGTLEIKNNNESDNTVSHSVRVVLSFPSNQTSGEIVLNTSHVLDTFKPSLAILVGTCSGYKDNETSISRGDIIVGQSVLNYQTGINTNDGLQFTLNDCIQANKSLLSALKLTDHSKSDCINYPSIEVSTIYKREWLLRAFFEYQDHKHNLGMSVWLREQGFDIKKTPARNTVVQEMFGKQYNNEKDELVKDGLLEEDENGIPNISDKGFEKMERLINRYGDYPYNLFNEIKPKIHFGQFGCGSALEQQYEFVNKKRISLAFKQCREKSNGIIAIDRDTHSFYLTTSNYKEISFISIKSVLDHADGDTDQNNNYIDYCHQISSSFALHLIRSHSFQSNEVQLICNFIPPPPLKSINRLGSDNIGYIQKISHGFKAKSTSVTISSQICGMGGVGKSTLAKQFAHHCIDNQLYKYIFWIHGETQETLLESYKKVLQHLKEEIKNQDNIVTAFASTIQQLQFKGNVLLIYDNVEDMSLIREKPQGNHINIIITTRSQVRNDRSSTLITLDLLNPNECKSLFKLWRPKISDTDVAELSRLLQRLPLAISHCMAYIDLEGIKEEEYIKEFEMFNDIQVIGDDSFTNPYERLIGKTISMAIGKMENKQDIESRATTLLHIMAFLNPDQIHSKVLEIILKGKDNRINAVVTILKNFSLISTDQEPNTNQNYNISIHRVVQRSIIVLLLQKYKNNNNSINLIYDNLSHINNQISTYFYSNNTNTNNSRNHINIRNSNSNSNSNSNSNSNSNSILLDNFSNLNNISIHLTNIQTIIQKMDSLPHKQKLELTNKLLLTQIIMECNHLRYLILNSTVNRKVLEALFLESNIDKNNISELVDSTFEFIHQNQQLTRGIDIKVALNIFSKFMSGIGISKDRCNIILMFIFLSLGKIGHFLDKDIQFFKQISTKRISKINDLLSYLPGQVLNFSQLQNMLTINFLASDHDYSNLIENLVHLNDKVKGLGPSSTEIIFNSFKSFGSDSTIIQYTTNLANENTNDNHVSKIFDILIKFKLTFGDDQTQIVINYIKELLENGILILEDYVNKLLEVLSKIKGFEKFDNDQIQKLFNYIKLLINEKFNDHQISKTISTISEQITKYKLHIDQIKKVINYTKQLNNNENMNSNHISSIISTLSEQIRIYRLNDDQIQTIINYTKQFTNEKMNRYYISKIILVISDQIVIYKLNNDQIQTNINYTKQLVNETMDGDHISSIISTLSNEIRIYRLNNDQILTVINYTKQLINEKMNGDHISLIISASSKQITKYQFNNYQIQNIINCTKQFTNGYNTYKIISVLSEKIRIYQPNNDQTQTFIYYTKQLINEKMDGDHISKIISTIFKQITNYQLHNDQIQMNINHTKQLINEKMNGDQILSIISTLSQQIGIHRLNNDQIQAIINYTKQLINENMKGDRISSIISILSRQIRIHRLKIDQIQNIINYTKQFTNENMNRLYIYKIISSIYKLNNDQIQAIINYTKQLINEKMNGDHISKIISTISEQIRIHRFNDDQIQAIINYTKQLINEEMDGDHISKIISTISEQIRIYRFNDDQIQTFIDYTKQLINEKMEGYHVSSIISTLSQQIRIHRLNDDQIQAIINYTKQLINEKVNRYHISKQIGIYRFNNDQTIINNTKQFTNGSKLSKIISIVSKNLSTIQFDERPQNGINLKKLRRSKRRTEYRSKFVSKYQITIVKVDLKQLINANIENDTTSTLSEQIVDYKLGNDQIQNKKGDDISEIISAPIPKIQLNNDQIQSIINHTKQFINENMDDRDISSIKSTLSKQISNYQLNNDQIQNIFNITKQLMNENMKGYHVSSIILTLSDQITKYKFNNDQIQTIIHYTKQFINNRKMNGYDISLITSTLSKHITKNQSNNNQIQTIFISTIQLINNRDINGYNISLIISTFSYQIAKYQLNIDQIQNIINYTKQLMNQNKDIYHISEIISALSEQISQDPSNFKECFKNNIILKVCSHLGFEYNFSKILQLYSNNPLAFEQSYISILSLNQNIESNQFLNFLEIMISCNSEERNYILAKFISEYRDKINAQTINLAQDKIMSMKEKDSCCLKNRLNSLDEYENDTNENDTETQVLFTKLIDVREHIQDHDFQILCRQLSFHPILLKESIIKSYIKIKKSLGLVLEHHDFQYLIFQSLYPYDDDQLELVCAIIEKTREHKQEYFCCNFHIANNIQRLYYYNYKFTIGNNNFLQVFYSQLKDIDNKNNLECMKTVLLNTLNPKIRFNANI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V439</accession>
    <name>A0A8J4V439_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Beta-1,4-mannosyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">GDP-mannose-dolichol diphosphochitobiose mannosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_005504</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>an N,N'-diacetylchitobiosyl-diphospho-di-trans,poly-cis-dolichol + GDP-alpha-D-mannose = a beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13865"/>
            <dbReference type="Rhea" id="RHEA-COMP:19510"/>
            <dbReference type="Rhea" id="RHEA-COMP:19511"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57269"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58472"/>
            <dbReference type="EC" id="2.4.1.142"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:13866"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000221">
        <property type="protein sequence ID" value="KAF2073187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V439"/>
    <dbReference type="OrthoDB" id="614844at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000030">
        <property type="term" value="F:mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026051">
        <property type="entry name" value="ALG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13036">
        <property type="entry name" value="BETA1,4 MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13036:SF0">
        <property type="entry name" value="CHITOBIOSYLDIPHOSPHODOLICHOL BETA-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="14">
        <location>
            <begin position="265"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="458"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="458"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2073187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="477" mass="54193" checksum="91287E0A007FB718" modified="2022-05-25" version="1">MNRVAVVVLGDIGRSPRMQYHALSIAEIPNTKVSLVGYRESEPHPLIVDNENIDISPLVPFPISMSRSGSKRGLLSLLVLWPLLAVSKVLFQCLQLFYTLWTIPSLSTILVQSPPAIPTVFILQVVCWMRNIKLIIDWHNLGYTLLALSLGKSDRHPIIVFAKFIERFFGRSAYAHLFVTQEMRDTLVRDWKLKGKTYVFHDKASPIFKNMEPNEQVAYLKEFIEKYHIKDQDKEFIENIIESKSASLMSSKVKHKPDPSVHTSLIISSTSWTPDEDFSILLDAIVKYETDNLQHKQKLLYIITGKGPQKEYYLNRISQLKLKKSRVITAWLDSQDYPKLLACADVGVSLHKSSSGLDLPMKVVDMFGCCVPVLAINFACIGELVKQDYNGLIFQDSQQLFVQLRSLFGSTSSYADIAGTDPQTQQKQSLTQMKQNLIKDRKTDTWASNWASIKHLFSKSSSSPSSYPTKKDSKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4Q6</accession>
    <name>A0A8J4V4Q6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_004921</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000183">
        <property type="protein sequence ID" value="KAF2073787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4Q6"/>
    <dbReference type="OrthoDB" id="73788at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3970.10">
        <property type="entry name" value="BSD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051494">
        <property type="entry name" value="BSD_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16019:SF5">
        <property type="entry name" value="BSD DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16019">
        <property type="entry name" value="SYNAPSE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BSD" evidence="3">
        <location>
            <begin position="210"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="64"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="301"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="20"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="275"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2073787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="458" mass="52553" checksum="3BEFDC19277FC162" modified="2022-05-25" version="1">MEFHYESIYQKQGEEKEQQSNSSVDSTTSSSPAATDTFDLLSKSIPTISSWGWGSFSSIVDTVKQKSEDIINTYKEEIQNQQVQAQQESLNNVNVNLNQEDGIIIEEDVSDDDGGDDSLLTSPIIQNLSVLDIKNKISFGFSKFLSEFPSAINNDNGNNSLEKNRDNFTLNNSSSNSKLTTTTTSLTTDIDEKDIYFYSNDPENSLYKQFRESFKLLDYQSKITQLLMNNSRVSKSYLYLVPSKVLEFDFWSRHFFKEHLITLTEKALQSKDTLGDEDEITWDNDDDDDENQNKDEEIIQQEKVEAEIENTGNDIKEEEEEIKEANQVKAEEEITEVKEDIVEPVIKEEEKEEKVVLENENDTSLIDIIDQPNDHLIQYDPLTSFSNVNNNNVNIQQQQQQQQQEETLLQPITRPTPPVTPSTIPNNTIPKKLSMVELNDWEVLSDKKSDEEDWGAWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V605</accession>
    <name>A0A8J4V605_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MutL DNA mismatch repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_006369</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000291">
        <property type="protein sequence ID" value="KAF2072314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="20909at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032300">
        <property type="term" value="C:mismatch repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.20">
        <property type="entry name" value="MutL, C-terminal domain, dimerisation subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.100">
        <property type="entry name" value="MutL, C-terminal domain, regulatory subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013507">
        <property type="entry name" value="DNA_mismatch_S5_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014790">
        <property type="entry name" value="MutL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042120">
        <property type="entry name" value="MutL_C_dimsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042121">
        <property type="entry name" value="MutL_C_regsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037198">
        <property type="entry name" value="MutL_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH, PMS, MUTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073:SF47">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08676">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01340">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00853">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118116">
        <property type="entry name" value="DNA mismatch repair protein MutL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair protein S5" evidence="5">
        <location>
            <begin position="247"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="MutL C-terminal dimerisation" evidence="4">
        <location>
            <begin position="1184"/>
            <end position="1329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="403"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="613"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="708"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="905"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="994"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="404"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="431"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="447"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="501"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="613"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="710"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="727"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="925"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1036"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1051"/>
            <end position="1076"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2072314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1375" mass="154919" checksum="DCE888E32D8DF72A" modified="2022-05-25" version="1">MDTSSNNPTMSNNNEDDQVSIISKLDDDLILKIRSSVVIVSFEQAIEEVIFNSIDANATSISISINFNQLTFKVQDNGCGITFENLVAIGERYCTSKIQSYEDLKTINSLGYRGESLASIADVAQLEIITSPKNSNNNDSSNNNNNSGQQLKVYTKLIEFGKCEYIRPRLGDSGLNVHGTIVKVKDLFKYYPVRRQSINQQNLRIICKKRIESIALSCPHIKITIYDETKSIEILKTPPDRNLLSYFTYFFGEDKAKILEYVCSAPNNGGAYREYSIQGYLSSPIQKGHPNRSLQYIYLNNRIVLNTKLHRYVNQLYQKYRSFNATRKAHGNASKLEKRENVDIYPVFVLFIKCPQLNYERSYDPSSKTFLEFQDWKGPFGEIENVVTKFLTKHRGYDHISCLSDSNNNNNNSSSNNSQRDDDSFIGGGYDDNEDDDYNDQDEYLDDNSNNINSSQSDNILDILPDSLFLPSPTKKKVTTTTTTTTTSANNSPKRSKATQSKPAKSKNSSNSSKSKVSSNPSIFPISQSSQLSSQSSQSSQSSQSSQSSQSSQSSQSSQQIKNIVSSQSTSQSSNSSIPTNSTSEASSSDGDMDTYHDLTLTSKNNIRDDYKYQPTDIHDISSSKQKDTATEKDKEKKDDFMDEDDDYENGAMVALPDSPITSGPASPDDVNESEPSLKLPPPQPKEDVNKNGTIVEKNDRYLIIKSEEGTTPPSTQSNPMNHLKDNNNNTSDNSSNNNRIENIIQQHQQQHAINPITSNQETKSIQKQEIIDLDNLDVTTITTTTTTTVKTVQTVVQKRSLLPFTPVFVKEEYPILIKKEDFHQEEIDIDNIEKENIKNSGSGNLFNLSVLESPDIILERTNTIEFNPTTTTTTTTINNNQCENCKHHNNNNNQIVVKNENGKQDTDMADNFGQLSDDEDDGQSSTPLSPSPSKKLKSSKFFSNPIKSEPVDYNLEKFKSLEEYSKIHPIDLDNEENNPPVTSKFFSFKLETPVKTEKEQEQPSTPPQQQQSILDKSDLFSPIPLSTKSPKKVSFNDSFNQTITFRKPDETDEDETEEEDNESEDEEDESDEIEKDQDMKDENNNNSTATTATTATTITNTLIPFNFKNHNVINTNTSIIPFNITTTNSNGDVLSPYFDTSLEKPPPEAPSPKVDVGAYIKQILSEQNQTETITKDRLSKFRFITQWDKKFLLCEAEGIVIILDQHAVSERIKLEKLESKYFGKDKSFDICPMPERTRWPLTKHEIELVELYSQNLLDWGFKWKSNATSINILQVPMFCLVGLGVNDLREFLYLLENNKGTSATKPPAAHRILASKACRTAIKFGQKLSQEVCQGLLKDLNHCQLPFQCAHGRPSIIPLLNYKKLYNFTKDLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6C3</accession>
    <name>A0A8J4V6C3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rab GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_003005</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000089">
        <property type="protein sequence ID" value="KAF2075692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6C3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001447">
        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="179"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2075692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="23747" checksum="7F786BB454A71919" modified="2022-05-25" version="1">MDPVLLRFIFFGQWGSGKTSASTRYCEDHYSDHYSVHVGIDFRIKRFNNLDPNMILQVWDKKTHRGSRTDIYPLILFRDANAIFVFFDVTNRESFDFVEQEIQQIPPLHDIELYIVGTKIDLETKRIISREEAIEFAQKYTAKYFEISAKENINVHQSFDNIVKSIFFKKINEFSESQPSIATSTTPTNTSTPTPITKSDCIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6X1</accession>
    <name>A0A8J4V6X1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002440</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000067">
        <property type="protein sequence ID" value="KAF2076262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="20820at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057709">
        <property type="entry name" value="DUF7949"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF29">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25820">
        <property type="entry name" value="DUF7949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035310588" description="EGF-like domain-containing protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1291"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="5">
        <location>
            <begin position="649"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="4">
        <location>
            <begin position="787"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7949" evidence="6">
        <location>
            <begin position="1018"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="3">
        <location>
            <begin position="1056"/>
            <end position="1262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2076262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1331" mass="148990" checksum="51285BA2C9AB9D0D" modified="2022-05-25" version="1">MKFIIIFIIFLSIVYSLEIKNYQYNEAKNNYYPYNGVCTKTLYIMVNSTGDTGNTTLGLSATVTSNILVSSFDRVFFYKTTVQVPSLNGIGYIDLSYFPPNGITQTFFNFTSYICPNQLPPLQIEHIPKFYPQLDLFGKSILAGKFKYDHFMQLNFQNYGLELVEPSFTLFPSDIAYNILKGPSLSIALIDSYGQAFPYNINTFVNSSYNLESITNLVAPSSCINVPYYHSYLTFRVDSVNNLYDLRADNGQVSGAKILLPIYGNPSNATYLMIGHNPDLSTPQTFYLYSFKSSTMAWENINSMTCTYKGFSVLPGLVSTTLSLKNVNIVRITVKATIFEGTYYLKREYSVDKTSVINAQYPFGVMGSDPLLMDFAFLFPTSNDLNAPYIVSFKKQYTNIQAQSNLNIGTVVSEPLPEMNSFEIQEGASEYIFTISAQQQSPNGYISMISITFDINRINLEILPRHLVAGTLSKGIFRRSFSKRELNNAIGNMVVSIYNHRLQVVNFESYQLFNKYGGVIPTNPNTMPAWPSFISDIYFKINDVNVTLGPVENSIYISLNSLKHDYVLSFTPNFNLHFKKYNNGPYLDEKTYFSVWDKIADCYRIDFTIPAKRFTGVIGYKIDPLNVDNVRLYNGFFSKSELRVVSENADMLTPVVTGVVVLPSQNVDVSTNTQISFLITIYDPSGFKEGLFTIKSNMDLQGYNFTITYANATAGDEQSSSYLLVIPIKPTDVAQKFYIAYARLEDKIGNVGIYPSYDDVSPLMDFDITTLPTIQTVALTPTDKTAPVVSLLKFTPDIINETTTSITFEFTVNEEDNGSGISLRHLPNIYLTSMHFEVLGNVCSIVTTHQDLNGINTLVTYKCVINIPGGYGAPLPVLISIYNIYDNVMNKRGYTAKELSDISLPYKIDSVINTYPILLSLTPITTSNQKVTIQGQRFGIDSSSTSIKVIQSNGNSYLINEFVLFTWDMIITDKINSFDGNNVGIQLFVDGVASDIYQLTVNIPPEIDPTSNENTMQCSLDSQCGGSTQGTCKNGYCVCISPYYGIDCTLKLVNNSTTSVDPGKPNVVIEYSTLSSLVSIYALREVDNNGQIVYETKFQNWNYSNSDNVFIYSTIVQTTEIKVTVEKYDQITNVTFAGTSIVMQPNSIKYTIDIGPYQFKSQLNILELVMKAEIESNDKDSCSINDSASDQEVDYCRVQINDVSLYGRFIRRGILDSRIRSISNKISSDTSIQTTNSTNSFIAINIPFYQRFVRIDPDFSVLIDSRSANTKQGSICAPETLKSNGLTAAQIVGIAIGSAVFLLILIAAVIFVIQKRSIKLRVFYFKAKRHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V840</accession>
    <name>A0A8J4V840_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_004063</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000137">
        <property type="protein sequence ID" value="KAF2074624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V840"/>
    <dbReference type="OrthoDB" id="16596at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861:SF1">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="3">
        <location>
            <begin position="54"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2074624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="256" mass="29472" checksum="6007637E35FD5B40" modified="2022-05-25" version="1">MEGNNIKNNEFSAQNIYDKDNFVEIYLETFKKPKVDRWGIFVSHLPKSLQGMNVLELGCGSGNSASFLVEEGANHVTGYDISQKMIELAKKSNEKNKNTTFEVKDLETLQLPEQEYDLVLSLYTFHYIFNYEGLIQTIAKSLKPGGKLVFFVEHPVFTASIEQPTWIERPTSGEKAWTVSNYFNEGKRVTNWISFDGVIKCHRTVSSYMQTPINHGLTLTHFEEFCPSAQLIEKDPKYIENLNRPQYLNLSYTKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8K0</accession>
    <name>A0A8J4V8K0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">THH1/TOM1/TOM3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003569</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000113">
        <property type="protein sequence ID" value="KAF2075134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8K0"/>
    <dbReference type="OrthoDB" id="15928at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="313" mass="35653" checksum="736E62F679637B35" modified="2022-05-25" version="1">MKYQEDHVKKCKQQFTNLKRLKTIPFLVFTSCAIGVVFRIATGFLAFPWDVSSVHYNRWVLALYAWAVQFYCFEFYFIALLWIKLAVVYFNGKDLPLNRIKMIDGIILGNIIVSLIFHTGWTVVDLVIFDKADMDAIWRIYFLASVLLLSVGLGYFGLKLLYLLSKTSKGIDGVLMAKIKYILCLTTFLGVYIWTLNLIFLIHAKNEPLEDWINLLIFIAELVNCFMTQFILGRSSVCIRLRILMGCPEMIDDTSTNASEMNASGPASTIEISLDASSISTNRNNSPFPSTIEFDRVGFSVDNNNNNNPDYQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V9F2</accession>
    <name>A0A8J4V9F2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VPS9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000063</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000001">
        <property type="protein sequence ID" value="KAF2078692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V9F2"/>
    <dbReference type="OrthoDB" id="21085at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.80">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037191">
        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109993">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VPS9" evidence="2">
        <location>
            <begin position="813"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="343"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="240"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1032" mass="116247" checksum="1CEDBFF6FF0599C7" modified="2022-05-25" version="1">MIHHVPKTPEKNKQASDLKIDLFDTIDQILDFSSTTTYKVLDSLIDLSFEKNEDLNSNSNNSNNSNSNINSNNNNNNNNNSNEIVQENKDIQSLISDIDSLVFDLNDIEDDLRKSCTRLYRLSRQTPSQEQQQDKEKVIESNSQEPSLPSPPSTPKPAEQSSCQLGQSFDISEVFSYDHSNSGPSSPSINDSASPISSPPLSPKILNIRTTSLNQIKTTTSNEISIKTTSAPSSPKWIHGNVSNKSSLSSSASSIKSLGSGGDQDLSSSVSTSISSSIGGSPLTLSGKLKNHIWRKSNQVGPVPNTVTKSPSSSSVHHPRSITDPSKFSFGRESIINLDSVNSNQNNCNNSNNNNNGLNNSGSFKHRSTFPGSIPNEDYKKNPTQILSNDIPVKSLVPPSSSLTNPVALSDSISSLPSPPLQSTLPCIIFDEKGFHNSDLKEELSKNPTKKRIDLENPTKVKKLKELLKNYRNRIISLHNRFSQEVEDLNERKYQQHQKYADKVRFFTLDHQYPIINLLRSYYSSKISCTIANIELDKKRNEFLKRRLKDISFLIKFLDENFKDSQSSNGGSPITSGGNSLNNSTNLLPQLNLNSCNNNIVKKNIIVYSSFFLQMEDLLDHISFSKFTKRLKVYNDKFISEKLNYKLYHLNHYKKQNEENREKIIKSIISQAKCHPDLPLYYPWDQNQEIQKSFSQIIRDSRFMEGKQIQQLLHLISDDCTNGKKIKAIDIEVFIEKFCERMLTSFHLLDGLSNSSAYYNNNTNNLNHNEEMEKEKFQKIFFLLNQLTKRSVYPEIYPLLKVILIKEVGGQSIKYDYDLCEKVSQFKTKSPRELNLLPTNFPEDYEWPKEIHQNPFHHAISTLNELAFFMVPIDLIYIIQQTINSIYSTCTNILNSLTFGENYQKILEKSSLLTANTRHKINVSHPSEVSCTSELSSSSSSESSFLKGGDSNISVCSASSNSNLSNIVAHSLSADDLIPIFIYVLMNSDIVGSSNMISHLIQLFSSELENSGEFGWCSVSFQAAISYLQQLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VAL0</accession>
    <name>A0A8J4VAL0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001453</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000034">
        <property type="protein sequence ID" value="KAF2077264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VAL0"/>
    <dbReference type="OrthoDB" id="22869at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38082">
        <property type="entry name" value="ENDOTOXIN_N DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38082:SF2">
        <property type="entry name" value="PESTICIDAL CRYSTAL PROTEIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="479" mass="54156" checksum="184EA2982443B6C9" modified="2022-05-25" version="1">MATTNSSGSCNVKKDPIMLYKNIDQLCKSINCVGKCLSQIINLYISTPFPSISSAELQETLNRFENETSQSIIDKKIEFDDKKTWSACSSDFFSRIQEMKSILNMSTTDVNVKQQNLNNYLLSLCKSSSNLSNYCKRQPLVNEILDIYIQTIVIQGICITALNAFHTTLDNQVNIESLKEEYHHVMIDCLKTAITATTTTTTKVNMDNAVGALLQSNCILYPAPLVAAPLNIPSAELKFNRTYSTPTHWVPDIQGEFIYRIDGANMLPDQYQEDLFSTRPMPNIDPFTKCYGTCIHLTNAINIRLTRKRNIALRIFGIYPKAIGNLTIGSDIKLKRLDCVQRVFPCIPIAFKSADATSFPEYCESTGARSGYTFTHTVKNVDTLLLEIGQEVKQQLQQQQQQDKGNIGVVKFIELIITDVSKQDVDMYNDRKSNYKRLLEQVDSIYDKLNRGIEINPEETKLLQNHTQLLSTLEDDALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJ56</accession>
    <name>A0A8J4PJ56_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010518</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001167">
        <property type="protein sequence ID" value="KAF2068155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJ56"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="201" mass="22335" checksum="044B47B864DA7DB9" modified="2022-05-25" version="1">MNLVSGPLAFYPQYYSKNICTAFMTISSIQLNTPTIVNFNPSISQNVNIALNTPYTKDPFLGSIDTIASVGTSSFNFIMNYKDNVNMNPFFLLLRQRPTLQGNQVDGTFLFFLNYKGSNYYDSNVDSDYQFSVFENNNQVVKATVHGTSVKGSQVVFLTDTSGVAAVPSDPNAAYLKMVYTMKSVSMILETILQLFLPILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJG4</accession>
    <name>A0A8J4PJG4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_010187</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000963">
        <property type="protein sequence ID" value="KAF2068487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJG4"/>
    <dbReference type="OrthoDB" id="5348404at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005178">
        <property type="entry name" value="Ostalpha/TMEM184C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23423">
        <property type="entry name" value="ORGANIC SOLUTE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03619">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01417">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="247"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="406"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="416"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2068487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="496" mass="56790" checksum="4D44F1BA177A9A2C" modified="2022-05-25" version="1">MIEDYFLKSDSFASTSPTATTTTDNTNNTHIGRSIIEGEPIFYSLFLIFGAFFTISCIIITGVLILCHFIHYNKPNHQKYIVRILLIAPIYAIDSWLSLYFKRDYWALFIDVARDCYEAYVLYNFFKLLTCYLGGEEAIYALLTRKDKQRLTFPLSCFSFYPKKNFYLICLQNIIQYAIIKPTLAIVAVILYKFRKYEDSNFSPSQGYLYITIINNISVLLALYFLVMFYEVFQVELNPHRPVLKFLVIKGVIFFTFWQSVLIYILIWFNILPSSKQYDSEKMGYFINDFLVCVEMFITAIAHAFAFSHYDYILPQYNDKLISSKHVGNSSGKSNGEVSLDGADLKNVSSSTASSKTSSFIQSFKSNFNRYSSNIKDGVGDTIGTDIVMDTIKSVKPLTIPKKKRSNKYQTFVDTGGDEEDFNNSSDDDDNDTQGQKFPNYFKIDMSHSNNNDENCNNNNGVNNNNNSTILTNSNLLSKSVIDANSYLIFDNSSNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKK0</accession>
    <name>A0A8J4PKK0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_009985</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCF family. Translational throttle EttA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000874">
        <property type="protein sequence ID" value="KAF2068692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKK0"/>
    <dbReference type="OrthoDB" id="15006at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045900">
        <property type="term" value="P:negative regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000183">
        <property type="entry name" value="ABC transporter ATP-binding protein yjjK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000011">
        <property type="entry name" value="Putative ABC transporter ATP-binding component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022374">
        <property type="entry name" value="EttA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008775">
        <property type="entry name" value="PRK11819.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43858:SF1">
        <property type="entry name" value="ABC TRANSPORTER-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43858">
        <property type="entry name" value="ENERGY-DEPENDENT TRANSLATIONAL THROTTLE PROTEIN ETTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="9" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="95"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="414"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="183"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2068692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="644" mass="72580" checksum="28F1B123060E0DFA" modified="2022-05-25" version="1">MLFVSKIGKSGNCKQLLRSYVHNVVPSITRQQQQYTTIINKNIGQINNNNNISSSTLLSSTKLTNINDSKFYSSNNNNNKNKKGGPQAPAEKTVISLVDVQKKLDEGRMLLKETSLSFFYGSKIGLLGSNGSGKSSLMRIIAQEDTEHDGEVLYAKDISVGYLHQEPELDEEKDVEGNIFDGIADKKEILDEYEELEQEIEELTGSELKKAQKRLKELKDQIDREKLWDLKRKIAIAIDALNCPPGHQSVVNLSGGERRRVALARLLISAPDILLLDEPTNHLDAESVLWLERFLKDYKGTVIAVTHDRYFLDNVANWILEIDRGTLIPFKGNYSAWLRNKEQRLSLESRKEEGRKKAINRELEYLSGGVKAQTKKNKARIEKYNELVAAAPERYREPGRICIPPCPRLGKDVFDCTNLSIEFDGRKLFENLNIRIEPGSIVGIIGPNGVGKSTLFRIMTGDLKPITGNVRIGETVKLGFVAQSRASLDPDRTIYEEISDGTDVVNMGAYQIHVREYISQFSFKGAEQDKLIGTLSGGERNRVHIAKMIKRGCNVLLLDEPTNDLDVDVLRNLEDALETFPGVAIIISHDRYFLDRLCTHIISFEGEGKVIVHEGNFASYEEERGKKNKRADPTKIKYKKLQTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL27</accession>
    <name>A0A8J4PL27_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009793</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000809">
        <property type="protein sequence ID" value="KAF2068883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL27"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="101" mass="11211" checksum="FBCC046A1052443F" modified="2022-05-25" version="1" fragment="single">ALSSMKSTNKRNTSELSIDALETPKAADKKMELFDEESETQNSTPRESISKEKIAPTQSSISFPAELDIPRVEEVKYVMKPVNSKLYLFPSGIRYPRSGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL82</accession>
    <name>A0A8J4PL82_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010061</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000907">
        <property type="protein sequence ID" value="KAF2068614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL82"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="156" mass="18060" checksum="97F7EE92BF7B8163" modified="2022-05-25" version="1">MHKLFNNNTNGEVYLRFKKPYYKLPKEGCLNPIPLSGKLESRSQGIQPLSLSNCFGTILYLPIRYFQRIVDKIEIDAIGYSFSTNNNNNNNQNSKKRFAIRLEAREGRINFMNIKGNIHLIGDVKFIKDTDHTLIITSKYSRNANNNQLIFKSKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLA7</accession>
    <name>A0A8J4PLA7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010448</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001120">
        <property type="protein sequence ID" value="KAF2068226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLA7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="830" mass="90148" checksum="88BC5B6F40EC51BE" modified="2022-05-25" version="1" fragment="single">LYPKDPSTAPSAVGNGSGSTTFIPNLYSPTTLDGFQDTTFADCPGDFDTNGPLFEILNENAKMKICNASENLKVLLVVSQSSLGSAGSYGVIFKQTAEKLEKLLGDKAENIFMVVTHASRNPHIMDQVTSDLDNILQQPTTSGIDDFKPILQQIIANKRFTCFCNPSEEAEDGSDYSPPSFSSVDLSRDALLQSIRGLAFKNVKGTTFSVSSSPNIQQMIDGYVNGTQVLLGAVSIVNQKVIQSLNAYIDLVNHQRVQVDISKLNDLLGKLTDICNTNDQKVSLFDYSKLLISKINACGYLPLINESEMNEILNCLSFFSLFSSNSIQINPNSSWKTSILLTNLYSSFINDIQSLMKSNLNTQDNKIFYHGYDIKLSDISSSVKSGVSDIQVVALNSIIIDCDITCPSANLSIYSPQWFIPSTAKFDLSSSTNTTKVEPSQGYKAQGNPGLAGYGGGNFYGVGEQFDKPLNLKIISQGGQGGQGGDGTQGKPAISGQRISINTENLSVDYSDPDTVRAAIESMHNNSEITSSFLLAFFNKHWFTNNSPLSFVLRSSEEGNKSQSDGCSPFDYINFHVDVTRINYGEDGGIGGQGGIGGLAGQIVILDLSINSNISSQFSCETSQGAQGVNGKHGRGGNQSHSLANVTVCFSHNRGWIYTDDGLPTATIGVNSLSDCSTHGSADPKNSLFNNNNQPQPIPHPNNAISSKENYISFIFQQNASFKQIDILSSQFVKNEINNNASGPNLDEIISRLFAFENNFSQKNNQDAIQCFYLQLQKDLISVAGSLTSEDPNIQTIKYLYQLLCTGISRLKTFNDSCVIDMKVFLQSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLE8</accession>
    <name>A0A8J4PLE8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carrier domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_010388</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001078">
        <property type="protein sequence ID" value="KAF2068286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLE8"/>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013968">
        <property type="entry name" value="PKS_KR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050444">
        <property type="entry name" value="Polyketide_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45681:SF6">
        <property type="entry name" value="POLYKETIDE SYNTHASE 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45681">
        <property type="entry name" value="POLYKETIDE SYNTHASE 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23297">
        <property type="entry name" value="ACP_SdgA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08659">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carrier" evidence="4">
        <location>
            <begin position="359"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="856" mass="97382" checksum="DBCD4E3EC3E37003" modified="2022-05-25" version="1" fragment="single">HNIGYITTDVSLYPPIYQRTALHLMFEAIANKELDLLPIKVYPVGQLNDALTFIGERKHIGKLTIDYDQDIVTPQLIKQNQNKIIKENNDINSNIKCLLITGQQGMVLETLKWILENSNTITDVIILTKSSMKWDIQRTINQHKSKVNFYFKSVDIGNSKEVHQTINQLYNENTKLPPVDSILHFAYLHTESEPLEITYGELSSVHDSKTMGLINLHEISLLCKWNIKHFIISSSVLVFSPTYNQCAYISANMVYDSFSKYRRSLGLECTNLIFGALQGAGYVSRKKSVESSLSHMGILPTNISKIIGTIGLIITNNKDIGTNLIISKFNFETLKKIGEKLIQLDYFMNMFESNNNTIKSQDSIRDKIISTISDLLSISASKLNYSSMKLKDYGVDSLFMVQLKNWIEKEFQKPNIISISQLQNSTIDTIIQIVQKSLKNNDTNNQKEVEKIQVNNNVEKMKDLFIETHDNEFWNNEIKLDNTVRPIESCVLKENYGRVLLTGATGYLGSHLLIDLLKQPKCKYVYCLVRSKSREHALSRIIQTLSKYQLIGLITQNDIENRIIPIVSDLDQPLLGLSHEEFNTLSQSIDVIIHSAATVRFNILYSQTHDVGNLKELLKLVSNNKERLIRFLLVSSISVFSIDNQSDGLDGNNIDKIPFLESINSHIGYGQSKIIQENIMVEAHNRGIPSVIVRPSFIFANPMTMIPNQNDTLQIFFNSCKSLKKCPQFEESDFISTISISSLSESIIRMALLDDHYWINENNTTIPLINLFGEPIMSKDLFESVSTHTSFPLIDFKEWVKHCLESTDPVCQRLGPLIPAVNPLLFKQLLNGVKSSDKDNKQTVTTDMILKLFPHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLI3</accession>
    <name>A0A8J4PLI3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Guanine deaminase</fullName>
            <shortName evidence="7">Guanase</shortName>
            <ecNumber evidence="3 7">3.5.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Guanine aminohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_009895</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>guanine + H2O + H(+) = xanthine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:14665"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:17712"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Purine metabolism; guanine degradation; xanthine from guanine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the metallo-dependent hydrolases superfamily. ATZ/TRZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.3" evidence="3 7"/>
    <dbReference type="EMBL" id="AJWJ01000846">
        <property type="protein sequence ID" value="KAF2068779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLI3"/>
    <dbReference type="OrthoDB" id="194468at2759"/>
    <dbReference type="UniPathway" id="UPA00603">
        <property type="reaction ID" value="UER00660"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008892">
        <property type="term" value="F:guanine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006147">
        <property type="term" value="P:guanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014311">
        <property type="entry name" value="Guanine_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051607">
        <property type="entry name" value="Metallo-dep_hydrolases"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02967">
        <property type="entry name" value="guan_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11271">
        <property type="entry name" value="GUANINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11271:SF6">
        <property type="entry name" value="GUANINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="8">
        <location>
            <begin position="75"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2068779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="463" mass="51987" checksum="9FD822AB7A58B927" modified="2022-05-25" version="1">MQDLYEIVIKGTSISCGVDINDNVKIKVDHLFCISRQGIIIDIIDPIGVDQQRYQEVLKEYREKGKCKELSGDQVILPGFIDLHIHAPQFAQLGTALDIPLYDWLNKYTFPLESKFSDENFARQVYQDLVSQLISNGTTTALYFATIHKSTSCLLAEICEKKGQRALVGKVVMDDVNQNPEYYRDSSTDQAINDTEEFIQKLLAIGSKSHQGIYPVITPRFIPSCTDEALSRLGQLAKKYNLYIQSHCSESDWEHQFVLDRFKISDTQALDSFGLLSNKSIMAHCGHLSDGDMDLFHKRGAAVSHCPLSNSFFGNAVTPIRKLIEKQVNIGLGTDISGGYSSSIYDNIKQSVISSRMLEDGTNKDLLPNVRGVPNSRITLSQAFYLATAGGGESLSLPIGRLEINYAFDCQIIDTTLNYLSQSSYFGKEESNYENLFQRIMYLSKPNNISEVWIQGKLVFNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLJ3</accession>
    <name>A0A8J4PLJ3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009586</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000750">
        <property type="protein sequence ID" value="KAF2069095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLJ3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="42"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="93"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="433"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="280"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="42"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="94"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="433"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="451"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="591" mass="69812" checksum="69D15B78B44C10E2" modified="2022-05-25" version="1">MTSHDPFKTPKFNKYRPPQLYPLDTFADDEEQQISNYEFNTRNIGLKANTPTINNQSEQEEKQEIPEYKPQHQYRMTKQIKEQLSQIAPHLLSQQQQQYRQRQQQRYIDSSEEEDVDDDGTGYMQQEEQEEQEQDDDQEPQHEEQEEEEGLTKSVFSDSEKAMYIKQIGDLRSKINDLAQVTVIFEEKEKNLQSELDQTKQFLSTQDEFIQAQKENIDALSIQIRDQNQNIIYLHAQVDGQYEEILSLEKKVQEQKHQIDHLISSTEKAQQLSKELVLTKSDLEKEVIKLRSENHELRNKSLNHSNNYDLQQQLKESNSFIENLSREKEQLLAQIEIELKEKQDLKYQLEELNSQLDESNVTRNELIQENMKSEQNNRELQDLLQQMDSILRQMPQNQNEQIEKMEIKQKELSDQVIEYRDKYSALQEKFTNQQFNNNNNNNNNNNNNNQNDRDQAKTNDDRQLEMAKKESTFISREFLQLKQLLQRIDSLMIVSSTPISKVMDPDDPLYFNSMYKDSMINGNSIFGNSTAARVSHPPDRMTSTQSTLLNSLKSIREEIITLKKYITKRFKEVFGDMDEVFESEPKSCIVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLN1</accession>
    <name>A0A8J4PLN1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutamate decarboxylase</fullName>
            <ecNumber evidence="3">4.1.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_009433</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Converts glutamate to gamma-aminobutyrate (GABA), consuming one intracellular proton in the reaction. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed to extremely acidic conditions. The ability to survive transit through the acidic conditions of the stomach is essential for successful colonization of the mammalian host by commensal and pathogenic bacteria.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-glutamate + H(+) = 4-aminobutanoate + CO2</text>
            <dbReference type="Rhea" id="RHEA:17785"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:59888"/>
            <dbReference type="EC" id="4.1.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the group II decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.15" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000707">
        <property type="protein sequence ID" value="KAF2069248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLN1"/>
    <dbReference type="OrthoDB" id="5152799at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004351">
        <property type="term" value="F:glutamate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010107">
        <property type="entry name" value="Glutamate_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021115">
        <property type="entry name" value="Pyridoxal-P_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43321">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43321:SF3">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00392">
        <property type="entry name" value="DDC_GAD_HDC_YDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 8 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="8">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2069248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="162" mass="18317" checksum="06F5A27C0F9ED9FA" modified="2022-05-25" version="1">MEEGCLLQSRGNTQAHIQYEDRQAISDALDQYEKETGIDIPIHVDAASGGFVAPFLQPDFVWDFRLQRVKSINASGYKYGLAPLGCGWIVWREKKDLPPDLFFIVNCLGGNMPTFSLNFSRPGGQIIAQYYNFLSHGLTSYRAIHQVLLKPYIDHFAIHPIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLS2</accession>
    <name>A0A8J4PLS2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_009311</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HscB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000677">
        <property type="protein sequence ID" value="KAF2069367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLS2"/>
    <dbReference type="OrthoDB" id="448954at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051259">
        <property type="term" value="P:protein complex oligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.20">
        <property type="entry name" value="HscB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004640">
        <property type="entry name" value="HscB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036386">
        <property type="entry name" value="HscB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009073">
        <property type="entry name" value="HscB_oligo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00714">
        <property type="entry name" value="hscB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14021">
        <property type="entry name" value="IRON-SULFUR CLUSTER CO-CHAPERONE PROTEIN HSCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14021:SF15">
        <property type="entry name" value="IRON-SULFUR CLUSTER CO-CHAPERONE PROTEIN HSCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07743">
        <property type="entry name" value="HSCB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47144">
        <property type="entry name" value="HSC20 (HSCB), C-terminal oligomerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="115"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2069367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="276" mass="32322" checksum="04DC23D577D057A7" modified="2022-05-25" version="1">MINNTKIISLRLIRDVTKPSLSSSSSLSLSSSSILNYSNRSFINASVANKDTKNTLFYQQAQQYRYYSNTNDKKIENWKKAIKDCWSCNKNVVETKHFFCPMCNVVQPPPQDNVDIFYLFDIEPSFHIDYKDLSHRFKNLQKQLHPDLFQQNSDKEQALSKNLSTSLNTAYNILKSPFLRAEYLLNQKGYDLYNVGDVDPEVLMEVLEVREAIDDGSEDEIKQIGHENRERINQCCKELESLFNNNQYQDALKKVVYLRYLTRIQEEVHKRLDVHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM03</accession>
    <name>A0A8J4PM03_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008217</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000488">
        <property type="protein sequence ID" value="KAF2070465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM03"/>
    <dbReference type="OrthoDB" id="439743at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="186"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="186"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="199"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1241" mass="143421" checksum="CBBCB4D543DA8832" modified="2022-05-25" version="1">MTIINGHLRWITPSVFKDNVLDHLFVYELTSDRFNQYEISNDIKYIKVIITESDMNNKDLKLPKIISSNLTDLELYFDFKYSKSKDYLSLKLKFKDFVIPENIKSLTISNNYKYDDGIHNLLLPHSDKFPSSIKKLVIPWLTKDQISFIPPSIKHLVLVNQVKPFSTLPTTIDHIEFVDKFSKVQKEDKYDEQDKNDQDNDEEEEENDDDEDDDDEEEEDDDDGEDEDEEDDDQDEYLHIKSIKNAKFPQNVKVTFDFLIDMKTPQKNFHYHIENPDEGVEDLFKSYLDVQTIPFTSCLKDKIYFHFSGYRRRKSLPNNIKYIVWTNDYFSDIDTIPRGFIKPSVKSIRFWSNIYDPVNWLPNTIEHLIIDSGWESFCNVQTLPESLKYLNIGNSDLGFDNIVVPEGLRHLEIYTGDWITLYNMIPKQITHLKINVIDGSKKRDHININLPSSLVHVDIDPKYIDNIKIIDEKEYYNPITTKSTLKKLHVSNSFNQMIPPNSLPPTLEDLSIGKSFNKKISKGVIPESITRLTDNTFNGLKIKDLFPNVTYYNGATLKRIELENAKEIEINSSVKELIKSKSLKSLTIIPHQIYFSLETLSTLYPNLEKLKILTTYHKIGPLPNIKEFSFKCSSTQIKFPDSIQILKASAYKTFTLEELPKSLKELRLGYKTNKSILSLNENQFNIDIYKNNNNKKIKTVESTSVAQTKPINNNNSNNNNQFLQIWRNIFLKKKIIDSLLNPSGQLIQDFCERDKSVQYISYTNVEIKPDTKKIYLKKDLLHKLYGSLPNHMTRLLSDKYHCYEKGTEIPIDTDILIWTLDEIIPQGIIPFGVRSIYFSSGFNQIILPDTFPDSVMEIAFGFCFNQNLKFVRFPKNLISLYFGEKFNQLLLPELLPETLKYIGFEDAPNNKVKESLPLLGPNVDHLLLHSVRYANNLNKFISPHIKYIKVGRKKEEKYIQVPPTVKCVTSYYTNIKVADVSLETTKTSSKSKKTIENNEISGSKLDQSIALDILIVEDIFIPPHHFDLLKVKSIAFDSYFDQNIFPGCIPNGVESVKFGENFNKPISANSFIKYSNGHSLEIFPSTIKYLKFGKAFNQPIVKGSLPSSVSVVSFGEKFVQVLEKDSLPTSITKLTFESGFYHPDLLEWIPSSVTELQIGKIDENREKEKTWNNIFPIEKLGNTSITKLILGDKEIIESPKLIPSNIKEIRLCKCNTIEHIPNTIEALILGNEHNYPLNQYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN50</accession>
    <name>A0A8J4PN50_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">HRDC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_008379</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the exosome component 10/RRP6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000511">
        <property type="protein sequence ID" value="KAF2070299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2250022at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071044">
        <property type="term" value="P:histone mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071040">
        <property type="term" value="P:nuclear polyadenylation-dependent antisense transcript catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071039">
        <property type="term" value="P:nuclear polyadenylation-dependent CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071036">
        <property type="term" value="P:nuclear polyadenylation-dependent snoRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071037">
        <property type="term" value="P:nuclear polyadenylation-dependent snRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06147">
        <property type="entry name" value="Rrp6p_like_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000059">
        <property type="entry name" value="Exosome complex exonuclease Rrp6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.80:FF:000001">
        <property type="entry name" value="Putative exosome component 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.80">
        <property type="entry name" value="HRDC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012588">
        <property type="entry name" value="Exosome-assoc_fac_Rrp6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021067">
        <property type="entry name" value="Glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002121">
        <property type="entry name" value="HRDC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044876">
        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045092">
        <property type="entry name" value="Rrp6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049559">
        <property type="entry name" value="Rrp6p-like_exo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12124:SF47">
        <property type="entry name" value="EXOSOME COMPONENT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12124">
        <property type="entry name" value="POLYMYOSITIS/SCLERODERMA AUTOANTIGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11397">
        <property type="entry name" value="GlcNAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00570">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08066">
        <property type="entry name" value="PMC2NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00341">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50967">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5" id="KW-0271">Exosome</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="HRDC" evidence="10">
        <location>
            <begin position="468"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="635"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="703"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="786"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="956"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="703"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="716"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="786"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="797"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="830"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="858"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="876"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="889"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="956"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="975"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1003"/>
            <end position="1019"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00043957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2070299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1388" mass="157810" checksum="319980CF87DAB1E1" modified="2022-05-25" version="1">MFNNNNNNNSNSDQSNQDNEKLEQFSKNLFSNLITATKISNNLPSEEDYSYYETFPQFRVKMNDIGKRLLNLTQNFVQSEDPKTNLSFVDTNDTEELSDRFNGIVDLVDGMIDKVDKTIDPKYSQNQSNIYISSTVLASQNNNNKSKTNEINMFYGSNISRPQSKFSDPVDNSNFPFLPKIVNKPNSMVDLDPIFEKSKIKEVSFGKIVRTKEQETIVFPHPYEYEIQHFNYTKKQTQPCKEILSRGLEETPYTWIENIRQLEELTEKLNHVEEIAIDLEHHNYRSYQGFVCLMQISTRTEDFIIDTLALRAHIQMLNIPFTNPNIVKVLHGSDSDIKWLQRDFGVYIVNMFDTGQASRVLEYPSASLAFLLKYFCGVDANKKYQLADWRIRPIPEEMIRYAREDTHYLLYIYDRLRNELFNKGKNQPSYLNEVLRRSRDLCLFKHEREILDATTHIVFAKKANLSYNAAQLNVLKAVYHWRENLAREEDESIRYVLPNQMMLAIVEKAPTTVQELLMLCNPVPPYIKLHAHAIIQEILKAKLLNANGGANDSGIKQPILNTGAFIPQLQSILSSQSNTYYQNSIKQMGSNQINQINLNNMNLNNTSIGNNNNSNNNSIVSPNLTSIDMNNISTSSINTSGRQSPVHIPLHANSSSPVLSTDQLYKTAKWLSNDNSFTSLDVNNLQPAFQSLINNNNNNINVLNNNTNNSSMNPSVRKSTSIAPLNQNNHSNTPISNTSLFDSSGEESHDDHERKKSRKIASMVASSFEPTTLAPTFKPIASLKSLDKQNDQHENNKDDDEDEDFDEEEEEEEEDDEEEEEEEEEEDVNMVTSQDNNTATKQSKKAKGMESDGEDGDLSNQVPKSMQEIYQLSNMNRRRNKEKKKLKENPNTSVGSTTTTTTTTTSGSSSGSSSLQTSPTLGNSSVESKASSKKDGENASVTDTFSFLKEIGWVDNEQTPDSFKPSNLNVPNHPSQSNQSGANNNNQNKVPLTKQSKQKQQHHNPNQQQQNQQQNQQQQQFVSFDYSNTNTMNTQSKNPNQQQNFFNLEYSGAKKKTQSSGAVRAKSECQWTIKNAYETADDPSSIFIGLVWQYDMNGNSDEKCFEVKFDDTISSKIKTIKLDYKEAKGPCYARSLAQSLYGGEKYCLQIDSHMRFIKGWDRILKQELDQCGQEDGKAILTCYPMGYTLPNKIPTYRYPILVVASHFGENDGMLRIRGRVVNAKLSKPVGSLFWVSGFSFSLGTVIKDVPYDPSLCHLFFGEETSMSLRLYTNGYRFYTPTQSIVFHLWSREHRPSFRENKSPETVAVEKKSLEKVVQLLTGECGSLDQYGLGSVNTLQDYTEYCGVDFKRQTITPKALYGGHEGDRDTFFMNDILEMVIKSSTGIND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP12</accession>
    <name>A0A8J4PP12_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007641</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000419">
        <property type="protein sequence ID" value="KAF2071043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP12"/>
    <dbReference type="OrthoDB" id="428342at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21581">
        <property type="entry name" value="D-ALANYL-D-ALANINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21581:SF6">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="106"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="609" mass="66841" checksum="02BDAEDE8557C40F" modified="2022-05-25" version="1">MTSQGKKIDIGANRNTPTNHPLGSTLSAPATPMVNNNNNNNSNSNNATNEFVQTQPQQGFSQSTGSMPRSQSYNFLAQNAASNVNPIPQQQQAATGFGTLEPSRRNPSLQHLNSSLNSSTGDMNSFINTSLNQSTSSSTSSYYSPTTAPYTNPATLTGDEATLKRLMKVGNWNNVFKVADLCIANTNNPLSILEFKLCRIISHVKMRSYKIAYDEIQGIGDNRDLVNCFETYPHLFPGRKGTMVPFSMRIIKAELACHLGLEKFQLDALYALLSLTKREIQNLDDLLKNSKANSKQNSSSSNNSNNSNSNSSIDTTTTQDKSQIYLSQLEATTAASLLTPNTASPKLPPQSNTTPNATITSPIAAAAPLLLSPLNTNSNNINEDVFESIGNDNSNESLLAIWKARERRITFSIVTFIIQKNRDFLLAIKILEDFMTRQGPDFYTLSAIGRLHLQMGNLKGGERVLLVADKLMNNSNNNAAECNLVSLMNKGFLSIANDQYPKAIEYFDKIIEIENSGGNINITAINNKCISMLYTCDLGGAITQLESLLQTEKEKSIDEILIFNICSLYELATDRSNEKKKAILADIAKRAPDNFDFKVFKISNTPSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP21</accession>
    <name>A0A8J4PP21_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Acyltransferase 3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008142</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000478">
        <property type="protein sequence ID" value="KAF2070537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP21"/>
    <dbReference type="OrthoDB" id="18003at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002656">
        <property type="entry name" value="Acyl_transf_3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052728">
        <property type="entry name" value="O2_lipid_transport_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161">
        <property type="entry name" value="O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161:SF0">
        <property type="entry name" value="O-ACYLTRANSFERASE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01757">
        <property type="entry name" value="Acyl_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035258178" description="Acyltransferase 3 domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="322"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="370"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="427"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="453"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="503"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="536"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="577"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="612"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="642"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="domain" description="Acyltransferase 3" evidence="3">
        <location>
            <begin position="267"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="678" mass="78484" checksum="F679BF54B24527D8" modified="2022-05-25" version="1">MNNQLIIFFFTLCITLSIATSSSGSYEVTQPCQDAVGGIFSLSYPNATMYLTYSGKAMNDLGNFDECLDMYNNTAQYCVYENTLLAEGDYVYYMGVCYPSVCTTEDVLELAPQYSLLPRNGTGTPSIHCYTDKHRDSKDYTRGSYAMIAISAFIGLLVLLGTTVDYVFFERIYKYQKKQHVTPNPNTQFNDYKPLLETFILNNNDHIEKREDQDCDDDDDKPLFYNRLENVINFESPNFFIKFLLCFSLIKNYSSFANSSSPKKYFDSLDGVRTLSSCWVIMGHTLLFNGSLGYDNVMYIISTARMGFAFQVIPAGEYAVDIFFFLSGFLVTFSVMNQLDKFKAKSCRSFGNLKFWSLYIVHRIIRLSPLFIYVLFFFMYIGSIIGTGPMYYLYDTVVNPMCHNNWWTNILYINNLTSVMSQECLSWTWYLANDMQFYLVAPFVIVAYRRNKIIGWAMIGFLLSFCFSITTWVSIKNQLAPFFVFSNRVLDSESFITDIYQKPWNRIGPYLIGIGIAFLYKEDKVKRIYKSQKIRYIFYLIALGLTFFFSYIPYSYYSTKGWNNIESSLFSAFGHTLFTVGFAIFILGTFYGYGGFLKWILELSIWKPFSKLTYSVYLIHPVIIMVRVLSSSQMFHYSWTEFAYSYIGNLAVSFGAAFVIHLCVEKSFVNLERLIFPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP27</accession>
    <name>A0A8J4PP27_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007144</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000364">
        <property type="protein sequence ID" value="KAF2071545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP27"/>
    <dbReference type="OrthoDB" id="2364174at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="188" mass="21773" checksum="0A33C2EC118288D5" modified="2022-05-25" version="1">MQIDSYSIQIYRSFSADWVYRFTNGTLVLLFRSICNTSDIKYFKLENVIPLPAVYTLPARLNLKKNQDKFTQSFEKIKAATGVEWSLDDASLESVFPHVGTYQNQVGDIFAEVIGYVAQNIEKRLSDEMVKEAFLELTPKAKLVFKFAEKLSTSNYWEYKFEDQSLTVYFKAIANTSDARDFDFEKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPA4</accession>
    <name>A0A8J4PPA4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cns1/TTC4 wheel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_008413</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TTC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000516">
        <property type="protein sequence ID" value="KAF2070266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPA4"/>
    <dbReference type="OrthoDB" id="420195at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21377">
        <property type="entry name" value="CTWD_Cns1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044059">
        <property type="entry name" value="Csn1/TTC4_wheel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46035">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46035:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18972">
        <property type="entry name" value="Wheel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="161"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Cns1/TTC4 wheel" evidence="6">
        <location>
            <begin position="266"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="875"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="879"/>
            <end position="894"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2070266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1024" mass="118050" checksum="C7DFAB9BCE16C498" modified="2022-05-25" version="1">MDPHQLTEAEEDEIWEASVERWKNYKETKRLEREEALKQGVELPEEDDDEWKQLPMFMEALPNEPTSNVHLTALQSISDECTPEERADAFKEVGNDFFKAGKDRYKDAIYYYTKALNVKCKDMRLNSAILSNRAACNLELGNYRKVIQDCTIAIEFNKENIKAYHRAARAYFALNKDKEALDVCEKALTVDPQHKDIKTLSEKIQKRIQDRERREREKIEKENAKRQEIELLATKLYQRGIKLGSPLFDMAQYTHSSDRKIAFDSDNIIHFPVVFLYPEYSMSDFIIDFEQDHTFGDHLSMMFPPDNPEYAGWDKSRSYTIDKIEVYFETNWTKPILPNVPFKETKRWIRIKHTTTIEAVLKHPDYIIPGIPIFYIVARVLSLSFNKVSSQTCMSDALLTSTGVSKEVDWWFIIKIRGFSDVYYYYDSDMDKKNEANFKIGYFLRSKFSAFGATLLPYANLDGNGERITTLGTNFIAYNDHFYTIKSKDKSDRTPDNNFYNRGAHEKGLIGWSTNVAGKGLIIQHSLPNFPYGYERGMFQPVMNDNIKALNGEPGYKGQEIKMYFTMYGWKTNIFGPNLMPYHIPGRKFYVTPEEAKPNVNDHGTRLQTGALQRPNVIGYTTTSQYELFSLLFTTTVKPSQQIFCSSLVDEPMSTSKPGTKVKEMVQFMSDLSDVGLYAGKFNEIEESFIEKGPGGKVTQHVSYPGKLKFKTRPSSNKPVFSEKEIKVGEYNYYMRINHQSDEANSGQGKVDDIWKSLIDSRKEPNLAITDQDTSQLNNLAISTWAYSNKDSWWKGKIVSPVFKINAHDNTFRWKGNSNQEHSKIAFRTKEIDSDQWNVCSSGGNLYYTGKDKIKSSLLICFKAKGLRQAFQNIMTNDGGKEDDKDTEISKDDGEHDEDEVDVQKPDKFTQKQSFIESLKIGKFKYGLSANVAHAKQISQEMDALKTTTRHFFNYNPSKQPDYTNVDINPDGSIPRRSPRLLGIDAEIVYLFHQPSRTDLLQDKIDLTVPSDIFYTMNPRKKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPS7</accession>
    <name>A0A8J4PPS7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007327</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MEMO1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000384">
        <property type="protein sequence ID" value="KAF2071349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPS7"/>
    <dbReference type="OrthoDB" id="417112at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07361">
        <property type="entry name" value="MEMO_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.830.10">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00055">
        <property type="entry name" value="MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002737">
        <property type="entry name" value="MEMO1_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04336">
        <property type="entry name" value="AmmeMemoSam_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11060">
        <property type="entry name" value="PROTEIN MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11060:SF0">
        <property type="entry name" value="PROTEIN MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01875">
        <property type="entry name" value="Memo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="290" mass="32858" checksum="999B77755ED79217" modified="2022-05-25" version="1">MSIRNCTHAGSWYLPNAQKLGTQLSGWLGNCKRINENVKAIIGPHAGYTYSGRAAAYGYVQLAPEKYKRIFILGPSHHVYMKNCGLTSLDVFQTPIGNLKVDKQVIQDLYNTGKFCWTTKKTDEDEHSIEMHLPYIAKVAENILDNITIVPIMVGNLNTELEENYGTLLAPYFDNPENFFVISSDFCHWGERFGYTHYNKTCGPIYKSIEDLDKTAMRIIEGGDPVAFSKYLKETKNTICGRHPIAVMLWTAKLAKQNYDIESLYYEQSSQVTDCHDSSVSYGVLSFTQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPU6</accession>
    <name>A0A8J4PPU6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Solute carrier family 40 member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_008050</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">May be involved in iron transport and iron homeostasis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ferroportin (FP) (TC 2.A.100) family. SLC40A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000466">
        <property type="protein sequence ID" value="KAF2070626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPU6"/>
    <dbReference type="OrthoDB" id="648861at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005381">
        <property type="term" value="F:iron ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009716">
        <property type="entry name" value="Ferroportin-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11660">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 40 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11660:SF57">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 40 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06963">
        <property type="entry name" value="FPN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2070626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="193" mass="21720" checksum="12B78794FCFC8054" modified="2022-05-25" version="1" fragment="single">MEVILNNQSSVEHNNNDNDIEQDNIALISKEEVEEDTFKNEYVDEAADEAHGIILSNIDLNESIGHNNNDDDDDEFVENALPKDIERHLSYYVLISHLITRSGDRAWSFIVPLILIYISPKSLIPTSLYGLSNTIARILLGPTIGDLTDRYKKLFVIRIGVLGQATAIGLSCALLKLIMDIESTELQRNIFYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPY7</accession>
    <name>A0A8J4PPY7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">S60 ribosomal protein L10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_007730</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000428">
        <property type="protein sequence ID" value="KAF2070957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPY7"/>
    <dbReference type="OrthoDB" id="10258869at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1170.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.300:FF:000003">
        <property type="entry name" value="60S ribosomal protein L10, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="entry name" value="Ribosomal protein L10e/L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047873">
        <property type="entry name" value="Ribosomal_uL16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018255">
        <property type="entry name" value="Ribosomal_uL16_CS_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_uL16_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001197">
        <property type="entry name" value="Ribosomal_uL16_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_uL16_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003239">
        <property type="entry name" value="PRK04199.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00279">
        <property type="entry name" value="uL16_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11726">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005590">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="Ribosomal protein L16p/L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01257">
        <property type="entry name" value="RIBOSOMAL_L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="217" mass="24778" checksum="121084DB2F8359F8" modified="2022-05-25" version="1">MGRRPARCYRYCKNKPYIKSRYCRGVPDAKIRIFDLGRKKASTDEFPYCAHLISLEKEQISSEAIEAGRISCNKYISKMGGKDSFHMRVRVHPWHVLRINKMLSCAGADRLQTGMRGAFGKPMGTVARVDIGQIIFSIRCKDSVSNHVLEALRRSSYKFPGRQKIVVSKKWGFTSFDRETYQRLKSEGRLMNDGANVKVITEHGTLAQYAKDIEAAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ21</accession>
    <name>A0A8J4PQ21_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">sphinganine-1-phosphate aldolase</fullName>
            <ecNumber evidence="14">4.1.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Sphingosine-1-phosphate aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">CYY_008666</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 16 17">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.27" evidence="14"/>
    <dbReference type="EMBL" id="AJWJ01000557">
        <property type="protein sequence ID" value="KAF2070009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ21"/>
    <dbReference type="OrthoDB" id="10254570at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008117">
        <property type="term" value="F:sphinganine-1-phosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000020">
        <property type="entry name" value="sphingosine-1-phosphate lyase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050477">
        <property type="entry name" value="GrpII_AminoAcid_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42735">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42735:SF9">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 17" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16 17" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="16">
        <location>
            <position position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF2070009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="539" mass="60800" checksum="E9D8946BCB7330B6" modified="2022-05-25" version="1">MIGVGNIKGVDLKTLNAFKGAPIHKGTIYILSGIFIYQLFLKNIITVSFNLPKINKSFLFQIIKKYIPSRYQQIQQEIDKQVDEIIKENFPDQPGIPSFNEIPLQGQSSDDILKILQDLSNNDIDPHQGKLFAYCYPTKKEHEELVIKANNMFIQLNALNPMAFQSLRKMECEVIRMSISMLKGDENTCGTMTTGGTESILLAIKSYRDRAKYLYNITEPEVVLPITAHPAFHKAGKYFSVKMVYVKVLDQYNCQLDLKDYKSKINRNTILLVGSAPQYPHGLLDPIQDIARLADKYKLPLHVDACIGGFFLSWMEMAGYPLPGLFDFRIPQVTSISADIHKYGYATKGSSVLLFKDQSIRKYQFLAYTEWPGGLFVSPSILGTRSGGNIAAAWASLVYLGQDGFKDYVGKIMKTSQAIQKAIRENHYVRIIGNPVMSIISLNSEIINVHALADVMESKFGWKLERQDRPKSIHMTLTPAHINIESVFIQNLDSSIEHLLDNPDLNNKGSAAMYSGINNIPITDIADEFLLEFLSKTYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ46</accession>
    <name>A0A8J4PQ46_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kish</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_006560</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Involved in the early part of the secretory pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the KISH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000309">
        <property type="protein sequence ID" value="KAF2072115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ46"/>
    <dbReference type="OrthoDB" id="10034655at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051523">
        <property type="entry name" value="KISH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009653">
        <property type="entry name" value="Ksh1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13229">
        <property type="entry name" value="PROTEIN KISH-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06842">
        <property type="entry name" value="DUF1242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU910717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2072115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="73" mass="8074" checksum="47D343ABAC0C29E0" modified="2022-05-25" version="1">MSAIFNFQSLLITILLFICTCTYIGATYPKLLEVREKHSISGLPRKAAIIGERLSPWVSASCIIMGCVLLWGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQA8</accession>
    <name>A0A8J4PQA8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator complex subunit 30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008516</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000532">
        <property type="protein sequence ID" value="KAF2070159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQA8"/>
    <dbReference type="OrthoDB" id="20514at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="231" mass="26678" checksum="B998EB8E318614A1" modified="2022-05-25" version="1">MEVMNLEENVCALANKGEKSVRTLLYSVLQIVKQLKQLQSDPVLLHPYLQSTAPVSTLFSEEELNINGIQVSSNINNTSTTSFVNDNDRIKELNDKCITQITKLKSIINEIISLESKILLEDDNNNNNNNSYQNNLHNIDNNYNNIDDQYNINMSDNNIDNSNFGYRNSNSNDFNYNNNRFNETPEQIQNDLDRLKQEAFEKNCIIKKLIDNMRLLQFSINSMNRTTVNNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQN5</accession>
    <name>A0A8J4PQN5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TIP41-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006658</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIP41 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000317">
        <property type="protein sequence ID" value="KAF2072024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQN5"/>
    <dbReference type="OrthoDB" id="10253878at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051330">
        <property type="entry name" value="Phosphatase_reg/MetRdx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007303">
        <property type="entry name" value="TIP41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021">
        <property type="entry name" value="GAF/PUTATIVE CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021:SF16">
        <property type="entry name" value="TIP41-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04176">
        <property type="entry name" value="TIP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="267" mass="30808" checksum="60C488BD09CAA0D9" modified="2022-05-25" version="1">MNTTAATHTKVPGGVKIRGWTVTTNKGSILKSTEKDEWEHQLALNELPEMVYGNNFVRLTHNETDLNIVFNAFDALSMCEKKADDSIKVSSSKKWQEINNSFGGAIEKSFDWTYSTPYCGSIIKNSQNLTNMKSDIIEETTEQIDIEKLKRPDPILFFDDVFLFEDELADNGTSMLSIKIRVMNDSVFLLQRFFLRVDDVIIKSFDTRIYHEFGQQYLLREFMVKESNYDIIKPYLEKDPTLATDVNFIYSKLPVKSVVTEKIIIHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQV6</accession>
    <name>A0A8J4PQV6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Follistatin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_006229</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000278">
        <property type="protein sequence ID" value="KAF2072453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="18761at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031160">
        <property type="term" value="C:spore wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003645">
        <property type="entry name" value="Fol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051417">
        <property type="entry name" value="SDr/BOS_complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033764">
        <property type="entry name" value="Sdr_B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23303">
        <property type="entry name" value="CARBOXYPEPTIDASE REGULATORY REGION-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23303:SF15">
        <property type="entry name" value="COLOSSIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17210">
        <property type="entry name" value="SdrD_B"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00274">
        <property type="entry name" value="FOLN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117074">
        <property type="entry name" value="Hypothetical protein PA1324"/>
        <property type="match status" value="16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Follistatin-like" evidence="5">
        <location>
            <begin position="440"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="Follistatin-like" evidence="5">
        <location>
            <begin position="496"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="Follistatin-like" evidence="5">
        <location>
            <begin position="526"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="Follistatin-like" evidence="5">
        <location>
            <begin position="555"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="domain" description="Follistatin-like" evidence="5">
        <location>
            <begin position="584"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="Follistatin-like" evidence="5">
        <location>
            <begin position="613"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="domain" description="Follistatin-like" evidence="5">
        <location>
            <begin position="642"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="domain" description="Follistatin-like" evidence="5">
        <location>
            <begin position="670"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="domain" description="Follistatin-like" evidence="5">
        <location>
            <begin position="725"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="934"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1321"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1693"/>
            <end position="1725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="934"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1693"/>
            <end position="1706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2072453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="3517" mass="387420" checksum="7019AA6C60EEA52C" modified="2022-05-25" version="1">MKLNYIKIINILWIFGIFGIIFFPQINAIDNNNNDNSLDSEIIIYNDYLESGFKDHSSVSFTSSNALFDLENKFPTYNNSKNSIHFKSLHLLDGFEFITNQFTFNSSFFSYLSFKIYTNNTQPPQLIIQFTNDGKEYGPTKSLLSTINLKSLPINEWINAKLPLPLIGNYNGFRIYHPDFQDIQQGSDIFIDLIKIHYSESYLLYKESIKHQILKLNNRRNLLQVLDDSSQALTTLIKNYHVYSAQADRSLGGDSGGHSFWTPEQNNLLNNQLSWDFDGDSGILSEYSNGKLILTGTIHSIQNPSDCSSFWKIYFEFEPVENYISNRKELGEFAYVPMGFVDPSTWKYYRPILSNSKWIGNGCNTEQSVLFNGLQYDMLMQMGVGANGKNGNLGISVWLLYSDKKLIDINIDLVNRPIEKIQQQIDQPLSQPINYNEPLGCENVHCPLDYYCQVKNDGKRYCLSNPIKPTPQPISYSTPQPIVLIQNQNQNQNENICSRLDCPKGFYCTVSNGIGYCNETSSSLSSCNEFNCPKGYQCKTHYKHKQAFKSCEPLHCSQVSCPRGYECFICPIQGIPKCKKITLTCDDVYCPKGYECHVCHQTGQPKCKRDFQTCDDVYCPKGYECHICKESGLPKCRPITPPCHNVKCARGYICSVTDDFDTVCIPDVPTCEDVLCPRGYDCIHNKKQQLKCRRRPDCGTSCLPGYQCVVMGDGEECLLIPPPRNCFHYPCDEGYVCKYQNDTITPICVPQTNPCLEYSNIYDISGYLWFDNSADGVKNVFESTFIQNAYASLLNPDLSNARDLYGNIIPASKTNNVGSFSFKNIVPGQFIIRFGNVPQGYTWSPSMGFGDSTSWVLSNGMTPTFTLFDNLPPPSINKSLDTIPLGSNRVVKSISIIDPFTPDRISGKCTVHFSINSIVGGLFAPTPFISTTSSTFGTTTSSTTGASTTTISMTTGPVTTRSSISTAPPTPTPGTTSSTFTTGTPTPIPTNRVLAIGKRVFIDSNGNGIFDQGDLPVPGVNIYLYNDKNQLVASTNTDFKGMYYFDKVPSGYYYVQLSSLPFGYVLSSLNKYPTDQTNTTGIFGVLVGSPGMVNNNNNYPVTASLIRPDVDFGLIEKSQTYAVGDQVWIDPDRNGVLDAGERGVNGIKVTLLPQNGGSPQTTFTDHNGNYYFDNLPPGKYKIEFGVPPGYIITNGINSASKTTAVFELSPTSPKVHITLPTESLEAKYFNDKEDLGLYYTPTYAIGRFVWNDLNGDGVYQSNEPFINGVQVSIMTSSGGPVTDIFGETVKPVKTDDSGRYLFDFLKNEEYIVTFTPPPGFNPSPVPSSGPSRVNSYADPTTHRTPPISLASFAEPTTVSDRSTGAKASLIFPNANAGFFKPDTYSVGDLIWYDLNQNGFVDADEPGVPNVKAILYDFDNQIAKDVDGKQIAPIYTDANGRFMFSNLKAGFYHIGFSELPQGYKWTEQRASGNFDLSLFDSRPNEATGITENFILRKGFQETRFSLPSDDVPDPYINDYQDGGLIPGTIEIPEIYAVGRYVWYDDNDNAIRDQSEVGTQFVTIKLLTPTLGVPTYLDGSPILPTKTDRNGKYIFDNIPPGDYIVEFSNIPNGYTFIDGPWPYPNRFTGRTTPFRLSKARSVPNDPSSLHYNKLIRKLLELYASDSSLEIPNPFGSASDVSGSDLAIPNPFGSASDVSSSSSIDSTSSLANEGAPLSDAGPPGTSLKDINPGGAVTYETFVENLPGFNLASLIATRLDPTRNAGIIKSLSYGFGNLVFYKNEIDEVVGVKDVTITLTDDQGNPVVDLSGAVVAPVLTKSDGTYSFDNLRPGQYIATFSTIPPGFSFFNPPLGKLNFTLDVSSPDVRTSVPSDNAPRSKYFNPNVDQELSVPNVYGIGDFVWYDTDQDGIYNANGSEKPVQGLTVSLQDVDGNSVYNRYGLIVQPIKTDIDGKYFFDNLPSGQYVVKFDKLDGYIFTLQRSHEPLPGLFSVSSSPDSKGLTGVIDLSPSSPYLMFPPIPGMVATVGDFNIDAGLVPSSTVIVPYAIGNYAWKDLNNNGIKDPGEPPVPNVTVTLIKSDGSIPKDINGNVIPPTKTDANGKYFFDNVPSGTYRVQFTDYPNDLMFSDQTKPIPSTGRTPSFGLYPTYPNVREATPTDGVQATYVDFTENAAFVDLKTVAVGRYVWTDLNSNGIREANEPPAVGITLYLYDENNNKSLVSTRITNDQGFYYFDELKPGVYTISLASFLQTWTVTKPNAGNGTNDSKAINNEISKFRLSPFSSKVKPTDPSKDPVKAPFIDRDENIGILKPFIYSVGRRVWYDDNQDSIMDSNEKGVEGVTVSLKNIIDASPSNYSYDLYHNPLVATTNKTGDYWIDCIPPGRYIAQFSNVPKDYKFTKPLADSVPNDLGNYFPVELLTNGYHITPFDPKIDIGAKNPDYVNHFINAGIIKSTKPLSLFAISGFAFYDPNENDIMEAGETGVAGISVQLLDSLGNNAYNSKGVSVPVATTDENGQYRFDSLLSGNYSIQFIGSPYKYEYTIPNSQLDVHSANITGDLLFESNPKVRPSIPSDGVVANFTLPHQDKGIKLTPTYAVGDQVILDYRDTTLSNGPFKGQGITVSLSQDGMPAVDFDSNLIDDVVTDANGYYLFDNVPSGNYTVTFSNIPDDWFFSEMRNSSDPYSRTFSIDFFLPNAQTVLSSTIPKYSGVVARFVDPTEDVLLVPLLLAIGDLTFIDLNLSGTQGNPIQKISNITVELLNTSLKPVLDAYGNTVKPIQSDSDGLYLFDDLYPGKYVVRFGTAPGYSFTDQYTSKDETFDSNPNPSGLTSTIDLRMNGPNVILFAPDNRTSSLAWNPTIDAGYVENNPTGYISGLSFIDYNADGIFNKLDTVFPNITVNLVDRGVVIQSTKTDLGGLYAFSGLDIGKQYQVFFSDIPQGFYPTVPVAKYAGSQIQYPTATQTDVNVGLLNPDQYCQDNPKIFVACFTKPMNASEPVLISIDYNAYSTPDNSGPSFPKTMMADNVQQQSVYGVAYDKVEDYIYSATYKKAGAAVNLRSTIYRVKQSKPMVSEVFLRLDDIYGNGWALRESAETLSYMTQDQGDIEIVGDYLYVAVLNKTTVAKIPLRVKPTINNIILIKMTHNCGPDNYRIFGLGWDGVNMFVGGICDGMNTKTIPTGLVTRIESDDKTLTDVLSFSFGYPRGYIQKRTYRKVIISGIILADWKYWVSNTTITGGLWPHPHISDITFIGNQGNMILSIIDREADAYQNTPAGDMLYACKDATGVYQLELGGTCGALTGSTTFKQPNMLGQGPGNGEFFNDNFLSSATSDVVWQHDETHWSSSVYIPGSSEVVGNFYDLYDINQAILKHHSVSNGSALGGVLIFTLIPTTNFGKLNGLGDMALNCAIPPTYIGNFVWDDLNKDGVQDSYEEGLAGVKVDLYFSNGTLIDSTTTSDKGTYSFLISKGSYRLHFNPPPGYVVSPIATSSGISPVPVVLVNNQANDNGDIIFTVDNYGVSNMNYDAGFYKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQY2</accession>
    <name>A0A8J4PQY2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nuclear nucleic acid-binding protein C1D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_007459</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a role in the recruitment of the exosome to pre-rRNA to mediate the 3'-5' end processing of the 5.8S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer and homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the C1D family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000399">
        <property type="protein sequence ID" value="KAF2071225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQY2"/>
    <dbReference type="OrthoDB" id="1421013at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011082">
        <property type="entry name" value="Exosome-assoc_fac/DNA_repair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007146">
        <property type="entry name" value="Sas10/Utp3/C1D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15341:SF3">
        <property type="entry name" value="NUCLEAR NUCLEIC ACID-BINDING PROTEIN C1D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15341">
        <property type="entry name" value="SUN-COR STEROID HORMONE RECEPTOR CO-REPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04000">
        <property type="entry name" value="Sas10_Utp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 6" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="129"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2071225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="145" mass="16631" checksum="5B94A5E88F330F71" modified="2022-05-25" version="1">MTDQVLPSDIQQDITNFENILDKLEGQLDPFFKVSLKEHQEKLTPVESAKLNITVAYALNSLFFMYLQTQGVSPHDHQVKNELERIKPYILKLKSMTNKTEEKPKMQIDSDAAKRMIDNTLAMNKKIDSTVSSDNSSSSQKKRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR04</accession>
    <name>A0A8J4PR04_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA repair protein RAD50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">CYY_007407</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SMC family. RAD50 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000394">
        <property type="protein sequence ID" value="KAF2071270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="18797at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030870">
        <property type="term" value="C:Mre11 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003691">
        <property type="term" value="F:double-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051880">
        <property type="term" value="F:G-quadruplex DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043047">
        <property type="term" value="F:single-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070192">
        <property type="term" value="P:chromosome organization involved in meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000593">
        <property type="entry name" value="DNA repair protein RAD50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004584">
        <property type="entry name" value="Rad50_eukaryotes"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00606">
        <property type="entry name" value="rad50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18867:SF12">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18867">
        <property type="entry name" value="RAD50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="14 19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0469">Meiosis</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Rad50/SbcC-type AAA" evidence="20">
        <location>
            <begin position="6"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="189"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="315"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="424"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="620"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="810"/>
            <end position="861"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAF2071270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1316" mass="152369" checksum="8F196428B280B082" modified="2022-05-25" version="1">MSSVEKLLVQGIRSFNPKDSSVVDFYTPLTLIVGSNGAGKTTIIECLRYATTGETPPNCSNGQAFIHDTKIAGETEVKAQIKLKFKSPNGKPIVAVRSLSLIQKLHKQEYKQIDSSLQSYNSEGQKVSKSFRCSDLDKEIPELMGISKAILKHVIFCHQEESNWPLSESSKLKVKFDEIFAAVKYTKALKAIKDKRKSLTALVKEYKLKLETIESNREHYIRNKNEKVKMENQLVEVAKKLEQMKIEMIDKQNLMESAKKVEDKISSLRNEVSVLEARKVEMEKVKNQLFQSLPEVLNETDEELLFMSNTFGDELATMLKLEKELSDNLMRLNQEKEEINRKLSDNNTEFGRLQAMVDQKNEIIENRDKQISEFITRYKMQDLSQITPPYQNEDVDRFLSLVSEKLKVLTASLTKIERQSKENLSKIQSEISEHRIEHQKISERISNQNNQIVGFEKKIKDLDNEVSQHTHSPDKLLFLEKQIKQEQQLLDEMEKELEEAQLEVSIRNLNNEKIQIEKDLENCRQVLKLLNLQSSSMARLNVKKENIESKSKIINQQLTEHTPMLNKLFGKELTHDELQNSNQLLTNIKQLIQSTKVNIESQKNICSLSKEHYHRTKSEKKLLLDQLAKKEDSLKEVNKRLDSNSIIFGVDYADKDINQEIEKKEHLLHQLEKSFILLESEDILYKEYALKAQEDHRCSLCKTDINDSELDLFVNNLKSHSDDIPSKIKAIKSQMQEIQNILSKITVIKPDFLLKKELLLAIPDISVSIKQVSEGLEISESTDVQESNVLSLLQETLKSSESLLLSIQSIHNIREEIKNIGQEILQEEIQILKNTNDPRSLEEIEKEIDFQSNSLKLVNTELERISNSFSKQNSNILEKKSNLINLKNQLTNIKSSSGIIERILNTKQEMLENIENSQKFIQENELLVQEKYKIIEDLKQNLANEEGSFEKKQTILQKEKNLFSTKIESIKFDQSKLSDASSCKERLFSMDQETNQLKSKLETIQNDYNIGDQTVQDIRKDLSQHNNIKRAIQDNIAYRQQNSNIDTINKQILRKKEHISQISTQENLPNTAELVREMNSLKTKYDTMMGQTSILTNQIENYAKELKRDYYNNIDEVYRDLRLKVESTEIIGSDLDKYYKALDKSLMKYHNLKMDEINKTIKELWLSTYRGNDIDTIEIRSEEGTAANKSINYRVVMIKGNVELDMRGRCSAGQKVLACLVIRMALAENFCINCGILALDEPTSNLDRANIESFANALINIIEVRKNQKSFQLIIITHDEEFVQYLSRGNFTDYYWRVTKNDKQHSHIERKEISDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR15</accession>
    <name>A0A8J4PR15_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cysteine protease</fullName>
            <ecNumber evidence="11">3.4.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_006649</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[protein]-C-terminal L-amino acid-glycyl-phosphatidylethanolamide + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine</text>
            <dbReference type="Rhea" id="RHEA:67548"/>
            <dbReference type="Rhea" id="RHEA-COMP:17323"/>
            <dbReference type="Rhea" id="RHEA-COMP:17324"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:172940"/>
            <dbReference type="ChEBI" id="CHEBI:172941"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67549"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase C54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.-" evidence="11"/>
    <dbReference type="EMBL" id="AJWJ01000316">
        <property type="protein sequence ID" value="KAF2072038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR15"/>
    <dbReference type="OrthoDB" id="2960936at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019786">
        <property type="term" value="F:protein-phosphatidylethanolamide deconjugating activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035973">
        <property type="term" value="P:aggrephagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005078">
        <property type="entry name" value="Peptidase_C54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046792">
        <property type="entry name" value="Peptidase_C54_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624:SF58">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03416">
        <property type="entry name" value="Peptidase_C54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 11" id="KW-0645">Protease</keyword>
    <keyword evidence="8 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase C54 catalytic" evidence="13">
        <location>
            <begin position="198"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="93"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="360"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="702"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="93"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="360"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="379"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="718"/>
            <end position="729"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2072038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="729" mass="85789" checksum="5E770FB452D1817C" modified="2022-05-25" version="1">MFSRYNHQQEVGSTSIHNSNNDIPHTIKTRAKLQKQKEKIQERNEKKSSIFNNPSEPCTFSKNPIWLMGKCYNNSLQCSYNAYQQQNQQIPPQYYQQQQAQPYPQIPPQYQQAQPQQEVQPRKSTGLFQNLLNRNKDDHHHQQQLQQQQQQYYQQQYYQQQYYQQQQQQQQQQQQIIQQPQLNLNNRNFINNVNSYHEMFLKDFKSLIWFSYRKDFPPIQKTNITTDIGWGCMVRTGQMILSRGFVRHFYENEDVYKMEQQRGDQKYRMILSWFSDIPSQEHNYSIHDIVYSNKLIERKNSKDFIETLDNVEEWFAPTKICNVLRYLVKSQKFDDLEIYVPLDGVIYKDYIKNLCQINNNNTDNSNNSNNNVPVQQEQKAQEEKNEECGEKEQVEQKEQPQPQKQLFIPRPSSDNLNSNPLLVPVEVEHLSPSSPIEPSPLNYHKVVNSNNNSQDSVPPTVNQWKSLIILIPIRLGVDKINPVYIEKLKQMLKLSQSIGMIGGKPKQSFYFVGFQDNQIIYLDPHFVNDTVDPNSVHFPDTYQGCIPQKMSFTELDPSLAIGFYCKDQLEFNDLCFKLLEIESEGFPIISIGDHCPDYQVENDDPFKLNHSMDSDQSYQDEQTFDDIYNDDQQVASKNNEDIDYYHQHSNNNNCNQQQPQQPQHSFSEFEHHDQYDNVDLEQLEQGTCLNVLSPSIHKVSLQSSFRQDPNVETPDYSSDSDLDDFTMVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRL5</accession>
    <name>A0A8J4PRL5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Golgi apparatus membrane protein TVP23 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_006642</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TVP23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000315">
        <property type="protein sequence ID" value="KAF2072052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRL5"/>
    <dbReference type="OrthoDB" id="2151161at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008564">
        <property type="entry name" value="TVP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019:SF7">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05832">
        <property type="entry name" value="DUF846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2072052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="216" mass="24476" checksum="B858BB49B9E7123E" modified="2022-05-25" version="1">MTSVNEFGFTDSSIDEGHVGIGMKSSFLSNENNNNSYNNNKRSPLSGLDHPIATLFHILFKVTALILYITKSFGSNFVMTFILCVLLLSFDFWTVKNITGRLMVGLRWWNQVNDDGTNSWYFESASPDQRPNPTESFIFWMALYFTPLVWLLFLILSLLSFSFNWSIITIIALSLSIANVYGYFKCSQDSSTSYATSFATKYIGQSLLQKAASKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRY3</accession>
    <name>A0A8J4PRY3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_006040</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000261">
        <property type="protein sequence ID" value="KAF2072638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRY3"/>
    <dbReference type="OrthoDB" id="16820at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051104">
        <property type="entry name" value="FAD_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46720:SF3">
        <property type="entry name" value="FAD-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46720">
        <property type="entry name" value="HYDROXYLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G01460)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2072638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="446" mass="50526" checksum="84593A063A79E773" modified="2022-05-25" version="1">MIHTKKVLIQFQRGYCTVSATPVHLRDKKILINGSDIAGLSLGLFLKKKGISCDIVDTNSTITTTTTQMVQNSIRKTTSEVKPNVGGVVVYNHVYDLLKKLGVYDQLLEKSLQLEGSCFTERDGAAYGIFNFIKLKQEKNPLSISEADMKDILLSECLKFKPSEGIVSIKKSTKIKSIQQSPNSNNNNNNNNKVMITFSNSPVKVSYDALIGSANSIENVDTVRQYLFDKLDKEPSFYKDTHYFSTIVKKCNQPDIPMINTQFAYEKKLVTFPISSTQVAISGSFFKPRLPPHNHKHLIFHEFYDMEDVNSHNIISFQDSATENIALGKVDEQMLKSYSLDNICLIGDAADKLPYDNLLITSIAIEDANQLADSIGQSTSSNLSESISKFDRQRQTRMTNKYLPIIKAEQSIIFRPDWLVYLFGKYYFKYFCSGKKQLKRLKSLFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS30</accession>
    <name>A0A8J4PS30_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Actin binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_006313</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000286">
        <property type="protein sequence ID" value="KAF2072367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10017054at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032432">
        <property type="term" value="C:actin filament bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051639">
        <property type="term" value="P:actin filament network formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21217">
        <property type="entry name" value="CH_PLS_FIM_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21218">
        <property type="entry name" value="CH_PLS_FIM_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039959">
        <property type="entry name" value="Fimbrin/Plastin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961:SF76">
        <property type="entry name" value="CALPONIN-HOMOLOGY (CH) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961">
        <property type="entry name" value="FIMBRIN/PLASTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143885">
        <property type="entry name" value="RGC domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="6">
        <location>
            <begin position="86"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="6">
        <location>
            <begin position="249"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GAP" evidence="5">
        <location>
            <begin position="675"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="32"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="350"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="236"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2072367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1304" mass="153498" checksum="C48F15F75BE126EE" modified="2022-05-25" version="1">MSSSPHRKSYRNSYQSNSSNINQLHHDIFEHDQKQQQQNHPDYNSYRNSMEPGNSSQQDINLVAPKIAKKDNVLHNAGAEEGSVHTFSEEEKVAYSKFITERLSDVKELAEKYIPIDTTSDQLFTACSDGVLLNKLMESLFPTQVDLKGLVIKNKINPFEVIQNQNIVIKNATKVGCIIVNIGAQDLVNATPYLVLGVIWQIIKSGLLSMVNQNAGEILEILFSDNEDQEEDQQEEHDQHDDKKDKDEHSAEEILMRWVNYHLERAGYDRKISNFSEDIQDSMVYAHLFHQLVPIEFHYLVSKVQEEPNLFARAEYITLACDKIGVKCFLAPSDIALGHPKLNLALVASLFNSEAAIENLRREREEKHRAELERVEREKERIQREREEMERAERERIERERIEREREEQDRLERERMERERMEREKEEQERLERERVERERVEREEYERVECERIERERIERERIEHERIEHERIERIRIEREKEEYERVEKERVERERVESLEREERLLRERMEKEAAERDRLAKEKLEFSKEYNICIRMQKRMVDDPSVTEDLVKLVGDITELKRNLVGEYRNTRALQKDLGKITRHIDRVLESKEEGKKFKKQASKKKIKPVKGVPSGLDPQKMKNYQSLFYYLQTKPHVMGKLLFLLKPNQVDKTFDNLLLSMFPYEISPREEDLFLRALSVAMEYQIKDSQNFTQLLTQESLPMNLLFEYFKKKGLKYLREVITPIILRILDHKDLNLNFDPFWIQKELDRELEIKDGKKSDKSSSNLTFEQLLQNPEIRNLYDTRSGQVIEYANELFVKILKSLDDIPVQLRYLCRVISEKSLDVFAASSPNERVEYVSFFLIIYRFINTIVATPDLLNELVLLQHPYISKQSRFNLAQVSRLVQSAFTKKPWTLSFPTASINNWIKEKSPQAEKFLMKVADVPDLDDLIHYTEYNEIMEENNIRFCTVINLSEIIWLHESILDFYYDLEDIGGLQEVNEEIVKKLVKSSSMTISEKRRYQTNLQRTKQQNTNAMWWLPSCLDALDSPIQMPEQGDRSIQLTFVNRQDTHDRSPRDSFKEAKIWMIEILSKVPHPKKDLNTIAILKDAKTFLRSGNSDSETIQLIGKADKLIEALKNFDQDLVTSKPDVYDVFLYEVEKELVSILRKNALMKSELTRLYSLLKQLRIDTSPIQNLVLFQAEQLEKAVAKKELKKSTQFHKHSLDKSEKFLLSELVKKGMIVRTDLPEATHNKTKVYIKNSSPGIYDIEAKFGPVSKQVTMELDQLLDYSKNHIDTYQLEGITLDVNMTLHVLMKTFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS47</accession>
    <name>A0A8J4PS47_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_005827</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the E2F/DP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000243">
        <property type="protein sequence ID" value="KAF2072850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS47"/>
    <dbReference type="OrthoDB" id="552115at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14458">
        <property type="entry name" value="DP_DD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000360">
        <property type="entry name" value="Transcription factor Dp-1, a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.80">
        <property type="entry name" value="Transcription factor DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037241">
        <property type="entry name" value="E2F-DP_heterodim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003316">
        <property type="entry name" value="E2F_WHTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038168">
        <property type="entry name" value="TF_DP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014889">
        <property type="entry name" value="Transc_factor_DP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015648">
        <property type="entry name" value="Transcrpt_fac_DP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548:SF9">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08781">
        <property type="entry name" value="DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02319">
        <property type="entry name" value="WHD_E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01138">
        <property type="entry name" value="DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01372">
        <property type="entry name" value="E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144074">
        <property type="entry name" value="E2F-DP heterodimerization region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="E2F/DP family winged-helix DNA-binding" evidence="11">
        <location>
            <begin position="119"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor DP C-terminal" evidence="10">
        <location>
            <begin position="271"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="52"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="212"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="391"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="273"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="218"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="391"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2072850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="443" mass="50540" checksum="E58EEC8188E0713B" modified="2022-05-25" version="1">MEAYEGEQSNEETNNQQVQQQYQQQYQQQQGNISVDLNNSFVINDLLTGLRNRPQQQPQQPQHNQQQERNEIPDNNFNNINRRVNMDNHPNHFNRHNENNNTIQNIPEYVEEEGGKKDEKSKGLRYLSYRVCQKVQAKKTTSYIEVSNELLSEYVEENKRNHPDADPSTFKTNTVKRRIYDVLNVFQAMNIIKKEKHKISWVGLPAGFPSNLVDDEPSSSPSNTQSSSQSSQQLQHSSSTLSTDNFSQETEISSSQSTPNPTTTASDRNQAYYQSLERQKAEILERIANKKQMLRDMEAQEKAYHALVSRNSSYEQKKDPVVANKIQLPFIVVNTKNTTVINCDVSDQRTKYFFNFTQPFEIHDDNELLKKLNFSPSPEISLPSTYSNVVPAAPVTAPNPTANSRPTTTTTTTTTTTTSTAAAPTNPQPKKKRKKNSGHHEDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS74</accession>
    <name>A0A8J4PS74_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RabGAP/TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_005813</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000242">
        <property type="protein sequence ID" value="KAF2072865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS74"/>
    <dbReference type="OrthoDB" id="294251at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000026">
        <property type="entry name" value="TBC (Tre-2/Bub2/Cdc16) domain family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF20">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="182"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="598"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="634"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="64"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="791"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="841"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="33"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="104"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="845"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="856"/>
            <end position="866"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2072865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="909" mass="103864" checksum="758EED3EA8A7A0A4" modified="2022-05-25" version="1">MSSIDNHSSNNDDSTIKSTETIIISNSGGNCHIINQESPSGSPTPHISISSFIKSSKSKLLVTNNNHNNNSLSIGKRGTLKSSTGDDSDGGSGSTTTTTSVIMKQDHQEQVDSKNEHIIEVKWRDQYGFVVEHFIPELKKFEAQENEKIQEKLLKWTEFIDQNKNVGLSSNPKQLVKLLSRGIPHQSRESLWKIYSGSFEKQRKEELELYKQQKEEKKSRFGTIRGLNPPTIRKSYFKHLLHIIKISNHKSGRFQSIPEIDKDISRTFPGHPYFDSDEGKRKLSRVLQAYSIRNRKVGYCQSMNIVAGFLLYVCNKNEEDTFWLLSTIVEDYCTNYYSTNLMGSQVDMAVFDELVKTYLPAIYQHFNNYDVSLPLLSAKWFMCLFVGVLPTEIVFRIWDHMFFECGIYGYNKPSSEDQWLVPNLITTTTKCHGLASYNLLVTGLSILSYFEQVILNCHSTPHLLVILSNHVKGLFNSKEFFKKYYQFRSIVDQDVFIRLKDRCEIEFITELEKMRKLRDLTNSAKVTRFSKTDLEKIWNGFRSLEFTNRYSMGKSMILDFKMFEKVFQYILIPNNNSSNNNNNNNINNNSNDNSNNNNRDLIIKQLFKVFDKNKDGTLDFTELMSGLCVLAKGTSEERLKLYFSFFDENNSGFVSKNEMKLMLQNVYDKIDMDPLAVTQYTVDQYVDMVFENYSIENDKLSFDMFRQAIIAHPKIFRCFMIEEETFSIEDFELSDLDTNSNGSSGIVGANNSSFRKKSTSRIKFQEALTRENELINEENFINNSICSSNNNNNNNNNNNNNNNNNNNNNNSNIEKVENITSSKTSTLTSNSLIEAIEMAKKDKTISPTSPSSNGCTTSLSSTATTSNEGSSTTVFVSATNNNKVGCATNNNINNNQEFQGNPLSCCKFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSS2</accession>
    <name>A0A8J4PSS2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RNA-binding S4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_005865</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase RsuA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000245">
        <property type="protein sequence ID" value="KAF2072822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSS2"/>
    <dbReference type="OrthoDB" id="440619at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000003">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1560">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042092">
        <property type="entry name" value="PsdUridine_s_RsuA/RluB/E/F_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000748">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluB/E/F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018496">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050343">
        <property type="entry name" value="RsuA_PseudoU_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00093">
        <property type="entry name" value="pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47683">
        <property type="entry name" value="PSEUDOURIDINE SYNTHASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47683:SF2">
        <property type="entry name" value="RNA-BINDING S4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01149">
        <property type="entry name" value="PSI_RSU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RNA-binding S4" evidence="4">
        <location>
            <begin position="60"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2072822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="324" mass="37269" checksum="67328CF2A0B7C80C" modified="2022-05-25" version="1">MIKSFIKCINTIFCVNPILNRGLYSRCNIGSRYITTSNIDNDNSKITSSLDNDNSIDKKERVQKYLARCGVASRRLSEEMIKSGRVTINDKVAKIGESFLIGAGDIVKVDNKIIRPSIPKLWIHHKPRNTLVSTKDSFGRDCLVEILQKVMNKPHIIPVGRLDYYSEGLILLTNDGELSRYLEHPKTEVTRIYKVRIFGQLTERMKLLLEKGIVIDDVQYKPIKAEIESETESNSWIILTLTEGKNREIRKILEYFNIKVLRLVRVKYGPYLLPSTLKPGEVIEAKLTGDLQKFVKKWSKDLRATEKLIKDKDFSLNQNKILNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT68</accession>
    <name>A0A8J4PT68_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005857</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000244">
        <property type="protein sequence ID" value="KAF2072846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT68"/>
    <dbReference type="OrthoDB" id="10553069at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="330" mass="37676" checksum="514B0B34B615A280" modified="2022-05-25" version="1">MTEGDNNNSDNSSQDSIQQIIGYNSYFNDDGFSPPPSYSQHIDKKPMISKSQRASRKKQKFKFITIIVAIFGLITMGCFVVYILFSTDKLGFKFLEAQCKLQSTEIKSTNITSPYDSSSGNINNNSVIGSDESLNTYDGVIGEGLCSPLNVGQWWYVYDKEYYKYTFFQSNSTMADNSTSNNIKQVEIIDSMNNKTITCYQGIYNVEYMANDKHNVTGIVYGLWTQHRDRVVDYLDQIKVDKSFSCYYNKYNESQIIIIKPPSSLINVLVICLFGLILVSIITTFFICLRRAYATSIKEKQQLLNENKLYFRYSSSDSIGYSSSNNNNNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT69</accession>
    <name>A0A8J4PT69_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zinc/iron permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_005399</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000214">
        <property type="protein sequence ID" value="KAF2073300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT69"/>
    <dbReference type="OrthoDB" id="448280at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF140">
        <property type="entry name" value="ZRT (ZRT), IRT- (IRT-) LIKE PROTEIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="89"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="97"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2073300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="363" mass="39114" checksum="68A138B8F60A5118" modified="2022-05-25" version="1">MVNPLQLKIISTVTLFVMSMAGGIAPFFMKNLKNSKRYLSWSNSFSGGVFLGAGMLHLFADSSEDMEYYTEKYNYPLSAFIIKGSPIGHGHSHGGHTHTPTPTHKSTPKLSPPKRKVIVDNEKQKLLDSQQQHQYKNYNNTTVGEDCSIDTTGSSSSSGSHSSCNSIIDDCEDQFSASHQQDISIPCGAISPHSSINYEKIEEDIVSPSPIENPVLPFLLVIALSIHSIFEGLALGIQNTESHVIDIMIAIFAHKILAAFALGVAIVTNTSRPSIVKLSLLILVFSLASPIGSIIGMIIVNSGGTETQLVSAILQGIASGTFLYVSVVEVIPKELNHTSQDIFLKSILLLIGFGGMALAAIWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTG5</accession>
    <name>A0A8J4PTG5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_005943</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the cytochrome b5 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000251">
        <property type="protein sequence ID" value="KAF2072755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTG5"/>
    <dbReference type="OrthoDB" id="260519at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000007">
        <property type="entry name" value="Sulfite oxidase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050668">
        <property type="entry name" value="Cytochrome_b5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359">
        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359:SF149">
        <property type="entry name" value="CYTOCHROME B5 HEME-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="13">
        <location>
            <begin position="52"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2072755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="128" mass="14888" checksum="8472547EEEF8D81A" modified="2022-05-25" version="1">MDQESITYIILFVLVALVIKKLFLSNDKPVVKPKQSTEKQKKKKRVVDRENPTFYTAEEVSVHDKEDDLWLIIDDKVYDVTEYVDKHMGGLAIMKNAGRDSTEGFNGEQHPIKVRQILDEYYIGELKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTH2</accession>
    <name>A0A8J4PTH2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Trafficking protein particle complex subunit 2-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009547</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAPP small subunits family. Sedlin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000738">
        <property type="protein sequence ID" value="KAF2069131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTH2"/>
    <dbReference type="OrthoDB" id="10258445at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14854">
        <property type="entry name" value="TRAPPC2L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006722">
        <property type="entry name" value="Sedlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044760">
        <property type="entry name" value="TRAPPC2L"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12403">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04628">
        <property type="entry name" value="Sedlin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="141" mass="16243" checksum="1438C0402A2070E8" modified="2022-05-25" version="1">MKKIVCVAIVGKNNNPLFIQDFSSGITEENKLKLHYIVHCSLDIIEDKPGPVKKNVPSDMYLGLLYPTEDYKVYGYMTNTKIKFIIVVLDTSDIKDSDIKIFFKRLQSLFINTTSNPFYKRNSKIESKKFLQEVTNLIPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU00</accession>
    <name>A0A8J4PU00_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006146</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000271">
        <property type="protein sequence ID" value="KAF2072529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU00"/>
    <dbReference type="OrthoDB" id="24459at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="59334" checksum="B3FE644AB613BDB4" modified="2022-05-25" version="1">MSSSSKEASTTLITNKKSDTMLFYYNNPSQPFRFELIPSHFDNIDLQLVNCSVASDVVLSKVVRVTLKNSKLNNGQPFSSGFIPLGVKSVILDNVQVSVDVLPDSVVELETYNLGQKINIPVSVTSFKAINITPENVGPLPNSVVDVTLSFDPAPGFLPSSVTKFVSTKPINVAALPTSVNDLRVNFGSHQTATIEHPSSTKNLEIWVDNGPFPKIRKSIESLRLVSILGSELTNELPSNLESLHIENLRNSPGKLPSNLKKLRLVFSEEATFDLPTGFLPTQLEELKISSNYKSSKVIPNFYPSTLTKLSLSDLKEDYGAEERILPAGTIPSSVIELELNLYCELVSGSIPSSVKYLKLGKNVKKFNFALLPDSVERLILHDNFYASEIARFPANLTHLSIYIDGSKPATTLPEGLTHLVLQRSVEDQPIPSTVSFLQIDFNPKSQIPDHIRQLRLVISDYYTNFTDLQSNYFPSSLVAVNFQSNKDNSPVFNVPISVTSISKRINPNLSNFGVEFNYIPFFNSFDCFRNDFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU17</accession>
    <name>A0A8J4PU17_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sister chromatid cohesion protein DCC1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009189</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DCC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000652">
        <property type="protein sequence ID" value="KAF2069491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU17"/>
    <dbReference type="OrthoDB" id="5199543at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034088">
        <property type="term" value="P:maintenance of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019128">
        <property type="entry name" value="Dcc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13395:SF6">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN DCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13395">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN DCC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09724">
        <property type="entry name" value="Dcc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="368" mass="42210" checksum="AE4E39E19D4F9B36" modified="2022-05-25" version="1">MDTTINVNSIEFSNDYPNQSYYLLEVTNEIVDEIKKENKSNLVIKGLPNEDAVLCTKDKTFIIKGAQTSNAILLVSKNNDKVSTLFQQHLELIETKPRLNSLHTLVESKSLKSNLEYEEQLESNSLGLSMNEILDNTQASENETLSYLSKINSFTLSNGNIIKLSDEYKMRIIDLIIAEITINSWSLEKVPIKDCIENLKSQVPKEILLQCINIYSLKADQSTTTEKEQEEDYFKFNTNLLCIDRAKQLLLLSSKGDWQYETFMENWKDSLSFDLKPTFDILRGLAIINVNKGSTQRFVKYIDQSTLSLLPKNRFKELFQLSSRWTIDTIEPYINTIIPPKTSLEQFILTYSRAISSPNGEKTIVPRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUC8</accession>
    <name>A0A8J4PUC8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation of fatty acids protein</fullName>
            <ecNumber evidence="10">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_004607</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>an acyl-CoA + malonyl-CoA + H(+) = a 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:50252"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:50253"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ELO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="10"/>
    <dbReference type="EMBL" id="AJWJ01000166">
        <property type="protein sequence ID" value="KAF2074078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUC8"/>
    <dbReference type="OrthoDB" id="434092at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF106">
        <property type="entry name" value="ELONGATION OF FATTY ACIDS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="237"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="270"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2074078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="316" mass="36872" checksum="E7BC42B511FB579B" modified="2022-05-25" version="1">MATVDYFSLVPTDYLKSLLPTEYDVSSFFKVDYSKWDWSQLIPSENFQYIEGVTPFSNKNFIFMAIFSYLFCVYFLKFLLSGSKVKGLSLQGFSALHNLILCIWSLGMFVGICYDLFLLFTQTEHGVNALFCSPKANPFSGRVFYWHYMYFVSKFYEFIDTFIIVLKKKPLIFLHIWHHSIVVLIVWTWLPEGVAYGSVGLFANTLVHIFMYYYFFRTSLNPSVRIWWKSYLTSGQLFQFTMSFILSIPFLLQDISISSVTGIIQHSCKGWGPFFFTMINNLIFLILFINFYIKTYLTKGGKSSPRSSRASKAKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUR5</accession>
    <name>A0A8J4PUR5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_004847</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000179">
        <property type="protein sequence ID" value="KAF2073851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUR5"/>
    <dbReference type="OrthoDB" id="20463at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16461">
        <property type="entry name" value="RING-H2_EL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045191">
        <property type="entry name" value="MBR1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22937:SF65">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ARK2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22937">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF165"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="434"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="243"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="290"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="317"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2073851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="478" mass="53217" checksum="3F92A397778D95FD" modified="2022-05-25" version="1">MYNNNDSNSSSGGGRKDNRGFSLGRGTSTRRAPSYKHHHTTTTSTTTTTSITNNGTVYSVSGGGYSGTKTSTTATSSPSINGIKTTSPFNTSPIKYNDNNAPKQFQPRISLLNTTNTTNNNIITNMINNNIKSTINKNNSNINNSNEFSLLSPPIQAPIPTLSTTTTTTTTTNLMNSSPFKSTNSLNSNISIIDELYQSDYLDDDKLAILEAALSSPPTKSPTKSPKLFSQNTLNETISPIKNTTTTTTTTTSPPMKNNNNNNDHTYKNPFTQTPTTTTTKTSTPPAYTNPFSTTFTNETLATRTPDIVSPKPSPKIEPSTPKTTTPIIPAFDDTDTASHELILQLLQQDIQDLGLDIDVRKDPYQFEYYPDEEEEEYYDENNDMSYEALSQLEPVRVGITEEQKKTIKEYRYNQYYQSRVSKSLQNEEDSKRCCICLDDYQDASLVKELRCKHFFHSDCIDTWLYGHNKCPACRDEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUX7</accession>
    <name>A0A8J4PUX7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004743</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000173">
        <property type="protein sequence ID" value="KAF2073956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUX7"/>
    <dbReference type="OrthoDB" id="185175at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044785">
        <property type="entry name" value="RopGAP1-5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23177">
        <property type="entry name" value="MKIAA1688 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23177:SF35">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN GACA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="2">
        <location>
            <begin position="146"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="335" mass="37222" checksum="46C725C5AA790ECE" modified="2022-05-25" version="1">MGSSSSKGTSPVESIDYKAKPTMNDLSHNNSNGGVEISKNVLDFNLGEKPCPLKCVVQDQFTIVNMNKAKLKFHLEPLQPKEFQLSFSPSSGSIDKGKSKTVKVKLIVNQKLNTNHKVVLKIEGGASHFLTVKIRCETGVFGVDPLSLECVDDDGYRVPQILVLMKRSLIDGGGLQQEGIFRLAGEQTEIKRLKESMNKNEFTSSTDINTIASLIKIWYRELPTPILNSIPTEKIFYSTDVDECVQAVNMLPEPQKGLLHWLMNLLLQVSEYSDVNKMTAQNLAIVVAPNLYDVSSSNPMEGLVMSQKCVQFLHNVLNHKVSVSRRESRSIALEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PV58</accession>
    <name>A0A8J4PV58_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">IPT/TIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_004515</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000161">
        <property type="protein sequence ID" value="KAF2074170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PV58"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1024"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="5">
        <location>
            <begin position="156"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="4">
        <location>
            <begin position="516"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="3">
        <location>
            <begin position="774"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="717"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="724"/>
            <end position="737"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2074170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1064" mass="118298" checksum="9CDEA1EF9BFD796F" modified="2022-05-25" version="1">MTIKSPLDHTIGNLTLNSFTEFRVPPYWGYFLDTTLTPTGQFPPINLNLVPLYGASVSSLKFLVKDASISPRQITYNKETRPFLRDIPPSYTISINNRLFFFTRTTTLLYRANIFNICTTKKYRFPIVFDASTKDLSYLVLTPAQTPIFASRVVTIIDDNPPTLDSLEIMVLNQTHSVVRLGASDDVSGVFMIDINNLLSETPVTKYHLVSGTKLNGVYEIIVPWKKSWPAQIQVYDYAGLSKFYANEQLNLMFDLDLQQSKSYHIGDITSLYLSKNQVDVTLSGVDVVLYANLSQTQQQRDLYSSIALTVALTKSTTLVLGVYNERSQLYEFPFKVPAKTLPAQVRYFLTINMQPFSVESDLLIGKFGAQARLNVINTGVIDTTFPIVTKVDQVPGQDQPLVWDLEFYDVSGVKKVIIGISSEYDFKGKNITLDGGGQTTFRYNLTYELDPSTCRAMNYWISYVYTEDLLGNIGESVRYSNIDFHPFYKFDDSTFDIISVGSSYCSLNIAEISSPVIKSLAYTRTTNSSIQNEQVQIFFKVTDDTKISLDHLPVCFFTDSNNDLVSSVARIVNNGNSGAIVDYICDFIFPFAYGPRALLSIYGLSDIYFNYIGFPSNDLRTLTFNPVYSIPDTSSLVVIESTSSLETSSDVLYIYGRGFKIGSTIVTIQADLEKFIEVPTITTGSVLVLNNLKPAIEYKIRVTEETSRESSLVVTLKGQLPTTPSPTPVTPTPTPTSPSCKTDCGASLGYGEYCSSTIANTTIEPNPHIPSVNLTLDGENGGTTNTKSKFSSLIQLISLKELENGGNDVVSKHVFNNNQWINVPSESNDPNVKTSKYKYIINNSLNTTIYSTVQVFPQAKSITFGKQQLQMNPSTVKFTFNITSYPFSKSTNTLQFIMAASILSSEQIGCSYQEFIQDDNNSQYLKIQIDQVSLFGRFIGYGIIDGREESITNSVVNQADEIATQQTSNSQQSYIGLNIRYFQQTALLDPDFSVLIETKSASDQDNSICSSARSSKKLSVAQIAGIVIGGVVFLFILCAVIVYVLSKKSSNPIALKLRRLAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVD5</accession>
    <name>A0A8J4PVD5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BLOC-2 complex member HPS3 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004451</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000157">
        <property type="protein sequence ID" value="KAF2074241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10255480at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017216">
        <property type="entry name" value="HPS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029438">
        <property type="entry name" value="HPS3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28633:SF1">
        <property type="entry name" value="BLOC-2 COMPLEX MEMBER HPS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28633">
        <property type="entry name" value="HERMANSKY-PUDLAK SYNDROME 3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14763">
        <property type="entry name" value="HPS3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BLOC-2 complex member HPS3 C-terminal" evidence="2">
        <location>
            <begin position="666"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="345"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="265"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1307" mass="147193" checksum="D3BFA2ACF28717CE" modified="2022-05-25" version="1">MVKFMSFDLYDRQNLLPPQSLEDDEPMDVISALNDDFLLSTKNGEIIHYVIGATNECSVLNRFNTQYNQILSLSYIATNDTILTLERNGEMGVIRVYQNWRQNQSYYHHLQPHLDGSCSPPTDVSSNNSPVIDQFNSSPTSSPVLGPTPTPTPIAPISIIQIPVPSSVIAANSCSITGRVIVSTKNTISVWAPVYNSSTLVSYFDKIFDIQAKDAIHVAIYENYIAYSTKIEAKVLYLNLYYKDPSLASENIMIAGLPNSSKSMNNINSNSNSNNNNQSVNNGPSEIVEDEHYFEITFDLNGNPTGRHQIPMLDNYNTLVESNSISSGLNKLSLASLSSTNINNSSNSSGIGGLTSTNSSSNSLSSSSLLGSETLGPINDIDHGINVNSESGYSVLSSTLLLLKKFPKDDEIHTFFFLPDSSTKDNLKDESSLVASAAGVNHHNSTPPMTSHSRNRSTSNLNIHLLKFNNNKKPNSLIGNMPSSIGNSNKGSRMIISNNNNNNTTGNSKTNNNSKLDKNHLLRCVISTAGKGFIYNLSKPSLLASYEYANDTILCTASQSFLYTINSEGLATWTIRSCEGGADDGDSPQPWGFNLTNFLYPSKVAVVGDHLLLMSKFQEESFSTPSVMMRGTASPNPKKADMSKGKKPSVSLWGLYILHHTPVQSLYDQILECAIKNKERDDEVYHQLLLEGHFLLQSKLANQHLFNLKVNDMASILNYGVERKNYQSLLKKSSSYLGEFFFKQEDYMRAALWYSSSDSDIETIFNLLVKNTNSHQSLIYYLENVLYDPQTYELLLNKEELCNRILEHYHLNSPHRLPMLILESSISCYSQVLAIDLLKIISEQCDFDLDELNKIYFALGLLYLDQNNIDDTITCFNKIPTNSLISLCLKNPKLLAQPGESSPTALCKILRQSTPWGLLELTTQLVKSREITPEFGLNILLSSTNTLSDGILNPVSYHHNDYDLDSLLIKIYLEWFLNIYYREYNSNNNNGNNSNNNNNGQNTLMYTSTSSISSSISMIALNEELLPLFIKYLIHLYVQDIHRFRGSDEKLFQSLNLDVSNIIKNNSGNNNNINNNSSNITSEFINQWIIFHLNTFFIVPQWLSKLPPFTFDIDNVSAKDETKYLLSSLYYKKLQSVLSINKIKDFEFMETIELIFSQDDEENLMLQSLKLACLPLLDRIKEAIDLVVNSNVSILLDYCLHFCKSLSDWSIVLDSVLRKYQDYYSTNNDNESITSQEQERETIIIEYEKILDHLSSTLDPESFLHLLPSNGRMDYFLSFIEKSFSNHQAKLLRSNLFNYLSEFENDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVE8</accession>
    <name>A0A8J4PVE8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF6748 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_004764</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000174">
        <property type="protein sequence ID" value="KAF2073940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVE8"/>
    <dbReference type="OrthoDB" id="18792at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040405">
        <property type="entry name" value="DDB_G0275255-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046636">
        <property type="entry name" value="DUF6748"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34411:SF2">
        <property type="entry name" value="DUF6748 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34411">
        <property type="entry name" value="DUF6748 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20533">
        <property type="entry name" value="DUF6748"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035256665" description="DUF6748 domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6748" evidence="3">
        <location>
            <begin position="233"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2073940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="434" mass="47091" checksum="29FC345C8689736D" modified="2022-05-25" version="1">MKVIKNLLILLLVACVFVVQADQDGFNGGRTFVPRHFGGRQGARPKGGRSIAGGNSDSDYDRNHKGGVGGGSGKGKDKPKPKPSGGKSIGGGHGKGDSSSDSDKDHHHLPTKKPTKPTTKPTTKPTTKPTFPPTYPPTDAPTIAPTKTPAPTQTPTSASPTFPPNQSPTPEPNPVFGQIQYYYFYYAPIQCFAPPCPQYYVVSCNSLDKKKYPILSAFFPDGVNTTDFLNQPDNMLVLYGALNECDEFEGNYEIHIQRAYKGLPYVGAVKPGLELYAIGDNGIRCIKAPCPSISARKVNCHFSANVNDYTEPYSKGVPFFDKSWYNGRFFTQDALLAQGTVVNGVFNVAHSFVMVPDPYNDCNDCEVPVCGQNTTAVYTRDFNRCLHSDGCTQVGACALFIPTCAPGYKLVSFPSRPFGCSAWICDPLFVEDTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVI1</accession>
    <name>A0A8J4PVI1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002981</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000088">
        <property type="protein sequence ID" value="KAF2075738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVI1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="38"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="129"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="174" mass="20834" checksum="9E6B70B9595F0545" modified="2022-05-25" version="1">MEINNNIIDDNTRLQQENWEFKKAGCSNKDHISLILQIEKKDKKLEKRNRIIDQLKNEKKNLIDSILKLNNGKTNEIDQFKQEIFKLFNDKNNQTMNDLQSLYFSFLEEHIKFKSKLETSLNQDLKWLELNNKKEMESLETKLKSLETNQALLKSNNDEKEKDIQRLKEKLIVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVW3</accession>
    <name>A0A8J4PVW3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA polymerase delta subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_003689</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000119">
        <property type="protein sequence ID" value="KAF2074993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVW3"/>
    <dbReference type="OrthoDB" id="514823at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904161">
        <property type="term" value="P:DNA synthesis involved in UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1030.20">
        <property type="entry name" value="DNA polymerase delta, p66 (Cdc27) subunit, wHTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019038">
        <property type="entry name" value="POLD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041913">
        <property type="entry name" value="POLD3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17598">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17598:SF13">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="220"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="220"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="261"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="336"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="365"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="378"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="443"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="473"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="490"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="538"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="562"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="572"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="588"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2074993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="601" mass="68559" checksum="F2097DA6AAA0DA6C" modified="2022-05-25" version="1">MTAQEKVKPILEKLRTILFDDSTPVTYKWIADEYSIHPNIAKEILLYFYRKNQDTLSPLFVVSGKLKEQNKVSYVLVNQDSLENSKKEFETLYGEHIYSLSLGNTGPKDPSCINTLSITSLKKKYETMVALGDDYFNKSVPIKSCLFEKRQLKRPLPISVVPVSYRHESQTKPVVAPAKPAAPKVSKPAGFSFFVKKEESEGNGNKMDLFSMFEKEKKEEMAKPVIKKETSPSKPTKKTTSTTKKSTTTAKATEKNSLLKSMEKKESTPTVKKEKKEKKRKSRDNSSDNEDDSDNGSGGSDNDPFADASDESDHMPSFNRKRKTISSGGASNSNLDDSDDDLNLNNDDLDQFTPPSQPMQKSFDDSENDDQEEEEIKEQEKKKVKLDKDKQEQERKAKEEKERKEKEEKEKERKAKEEKERKEKEEKERKAQEKAAEEEEKKKKAAAAKKKKIPLAVVQNDGEDEEQEEERKQKVKKSPAKKKVSKKVVEEEDEEEEPQEPIVVKKSSSKKKVIQEESDDDQQDIEPVVTPTKKKIQVKKEKEEEKETTTTTTTTTKKKTPVKKEKEESPKKPAKASSKKKAAAQEKGTQTNIQSFFSKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW08</accession>
    <name>A0A8J4PW08_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004908</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000183">
        <property type="protein sequence ID" value="KAF2073774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW08"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="82" mass="8435" checksum="1E7E6C760276F49A" modified="2022-05-25" version="1">MKIGSKSWLAIIGGIFLLFTPCVVYAASSSKELKRKVSEMPPPFESSVSSSSPKVSSSSAGCPSSSSSSDPSNDKQSLSTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWE3</accession>
    <name>A0A8J4PWE3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003826</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000125">
        <property type="protein sequence ID" value="KAF2074871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWE3"/>
    <dbReference type="OrthoDB" id="60283at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052050">
        <property type="entry name" value="SecEffector_AnkRepeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586:SF4">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="723" mass="84469" checksum="78B92525DED5118C" modified="2022-05-25" version="1">MKDRHALLREVFNHKFIKTLIFSFIERDNAQIKAYKYDDVVSLDWMVQNKQWALLRYKMNRLEYIHFDMGKSRELIFGIRDLDLFVKVYNRFKEFFVAFGRGVEYAARANNTRIVKYLVEKMPDNYERALEQCTLNKNLDLVRYIMDKSEKKKILKMPGIITTNLRTAIENKDVVMIKYLSQFNEAKQTEEYKVILLKIATATGSTEIFELVYRLYKAGVSEQWLLPSVPHYDLFVYLVVNFYKYIGEDLFFKLARATVQTRSYKSLLFLKQSNHLTPQIYKFVSNLMAVEAFREQDIDAYNMFANGVPCKMNTLSISSIKDALSNPARVKEFLKMGTSVTSYCFQKASSDPSTWETFMYLWECSKKEKMPLTTFISRKSTSITNRHIFLLEFLIEQDYNLQNEHWFWSVLAHNDENFAFINKFLSVYTISFPSKKDVLVGLSNAARYGAVANFKLIYNHLHQAGFINSNDIIQAYTNAIFYSHYDIVQFLVNSNAPHLNTMLGKCRTVGMAKLLLESGHSFTSYTIRDAVIGANISVLEFLLSALLDIPASASTYPMLEALKVSVQQNRVNHFKLLLAHYNGIKVGELVQEIGEHGNVDILKEFHNKCPSDLRSFASECLSKCIQNGHLFMMKYLISHFNRSLLNALDFESVLHYKYYHILEYFREPLMDYHSNISKLFNQAYLKEKDSIKIDGKFSHFIQKFIYNSQKKNNSPFFKKHMFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWH0</accession>
    <name>A0A8J4PWH0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exocyst complex component Sec8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_003647</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SEC8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000117">
        <property type="protein sequence ID" value="KAF2075037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWH0"/>
    <dbReference type="OrthoDB" id="272977at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090522">
        <property type="term" value="P:vesicle tethering involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039682">
        <property type="entry name" value="Sec8/EXOC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007191">
        <property type="entry name" value="Sec8_exocyst_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048630">
        <property type="entry name" value="Sec8_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14146">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14146:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20652">
        <property type="entry name" value="Sec8_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04048">
        <property type="entry name" value="Sec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="3 4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec8 N-terminal" evidence="6">
        <location>
            <begin position="47"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex component Sec8 middle helical bundle" evidence="7">
        <location>
            <begin position="296"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="237"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="464"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="572"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="801"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="914"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="245"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="362"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="464"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="490"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="580"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="801"/>
            <end position="815"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2075037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1154" mass="130732" checksum="14275E9B88262EB0" modified="2022-05-25" version="1">MEDEDKKIDENKITKVLSHIPEQYHKTSFQARRTALDYMRSTNRRELLNQIENWLDEVNDVTDDIVNVYFQGFNKSIHNYSRILEYMGESHSNAISMSKEVEEINKLIYFNNLGIERLWRRNLEYHYMIEILEKMEELKTVPELLDKYIKGNHFVHASNLLVNSINTLNDKDLINVNALMDLRQSLNERKEQFKDMIIDKLNDHIYLKTALSLKMVEEEGDQDNFNNFQSNLKKELLSPNSNSINSDQTQHQQQHSNNQYPSLKGTGKKEENKNNQMLKLEEPSKQSNSLEDLNINPESNGKLFLTLLVESLNVLNHLEAGVGLILGKISIELKAIINQCSGQVTNIYQTEGRVVPRMPGESLNSSTGSSPSSSSSNILPSSSLAAIGSNGIAGANNDDIAFTFLTDTFNRNDLLSNFTLNIPLVDLLKMLFTKVNMVFKNHLLASKVFNAAIKATQVKAKHYNFDKEDDHPDGHNDESDDGGGAEQERGLSSSVKSNGADQDIPDVYDSGLVWEIIQKELREMLRIHLHDTSSLLLSTNPSTSAANTSSDIGKASRLFSFSNSIVTDSWNGSTSPSLMSSQNNGSSTNTNSQSLSSPVMSSSNPSGMFKSSQYNVTPVYPMIVKFTDKIEKVIKDKDKAASGVTPSFVVSPKLVNRDIGVEKGLLRLYIDDFVHRNFLQHIKNDYKDRVTAAIEGTEAFKPMDRYKLVLRLKETKPILNSTLHIFQFIAELFSDIVAMSHYVVEFGAIIQTSLLRYYEKCLSKVSQEVDHTLTGTLMNTDLFKFILTSFEAHQYSLQAQQQQSQKEQQQQQQQQTAPHSSKVNKQFDMSKFQDTKEEEYELKSESELFSNAEKPVQRSQLILDIEKLTMLANMSYSLEWLADKVSQLFSQDHHLGGNTPDIVGQKGKHLKTPMKNQLGNIGGGGQKSGLASSRSGTPNVNNPNVVLSPEAIESLKSMEFTIKDICARFKELSRKCLVSLRIEYRVHCFYFLEGFKKTSYICEEERTDPDGFIVELNKDLSSNEEVMSMYLGSDKCHFLFGGIAKLIGKLLITRLQCIKQINDNGVAKLCKNVFTLQQNLSNIIVKREIYFDRVRQFYQSLASEDETLNYILEKLYVPFFSVEEGKVILDFLTSTKRVSSNALQTIEAKYKNMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWI9</accession>
    <name>A0A8J4PWI9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004643</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000168">
        <property type="protein sequence ID" value="KAF2074029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWI9"/>
    <dbReference type="OrthoDB" id="10659250at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="83"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="179"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="266"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="312" mass="35022" checksum="B6C15583858CB4BE" modified="2022-05-25" version="1">MMRVLVIFIPKSLPNRCIHFKKLGLWIIFQTQTKDDQQENNNNINNENIETQEINHDHYTEEEIRNNPSSFYDSPSTSNSSFSSTSSISSFSSSTSSNSNLNTPPIEFTPTATSASTSSTTNNTTASSSTNKSFKKRSWRIRTPSPRPKVVNTNYVNSSELNIQLPPTPTYPRKPSYITSSSTSSSCSSLNGTSSNSSTSSLSSNKSFRSRYNQSPIGSTMITNTKDNYICNEQSNNGQQQLNFTERINTSSILSKPCPIKPNPNPQQSQQPKHNSNWSFSPKKLFRSNSQQICRDMPEQSCYQTFVDNEIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWS4</accession>
    <name>A0A8J4PWS4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Abscisic acid G-protein coupled receptor-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_003478</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000109">
        <property type="protein sequence ID" value="KAF2075217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWS4"/>
    <dbReference type="OrthoDB" id="264392at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025969">
        <property type="entry name" value="ABA_GPCR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022535">
        <property type="entry name" value="Golgi_pH-regulator_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015672">
        <property type="entry name" value="GPHR/GTG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15948">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 89-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15948:SF0">
        <property type="entry name" value="GOLGI PH REGULATOR A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12430">
        <property type="entry name" value="ABA_GPCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12537">
        <property type="entry name" value="GPHR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="278"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Golgi pH regulator conserved" evidence="8">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Abscisic acid G-protein coupled receptor-like" evidence="7">
        <location>
            <begin position="130"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="90"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2075217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="310" mass="36533" checksum="9A4159D1299E6690" modified="2022-05-25" version="1">MAILSGYGAVNVPYMYISYFLRPVKDSSIQKLERQFLHTLEKIFSKKKRLLLVKKQLLSSRPDYSRRGNHYYYSSGFFSWIKNLWDKRSRTALSEDISRIEQEIKELEDLNRDHFCQIHELKLEKERIKFSTTLQGKFYNWLGYFFSIYCIYKVIMSALNILFDRKSGLDPVSRGLDLLLRYLHIEVDVPFWSQHISFLLIGLMTASSVRGFLNQILKVFHEYSSSLSSNNMILALAQVMGMYFISSVLMMRTSMPNEYRVIITEILNDIEFNFYHRWFDFIFIPSSLITTFALIAMSIKGSNALHDINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ28</accession>
    <name>A0A8J4PZ28_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Leucine-rich repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001851</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000047">
        <property type="protein sequence ID" value="KAF2076823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ28"/>
    <dbReference type="OrthoDB" id="18598at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034315">
        <property type="term" value="P:regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051279">
        <property type="entry name" value="PP1-Reg/Actin-Interact_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24112">
        <property type="entry name" value="LEUCINE-RICH REPEAT, ISOFORM F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24112:SF28">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="794"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="811"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="832"/>
            <end position="841"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="847" mass="93777" checksum="0F5D22A88D447D8E" modified="2022-05-25" version="1">MSSSSSPEEALEILDQDQAYINEQFSPLGGLFGEEITCQCPVYVTRDDKNLQLVPRILVIGKYKLVIIKKAKFKKTVKKSIHLYDLVEICEPSQDKLDALQQHSIDTIEIKYKDTSNNTFSSFLVKSPAKTIETIIIKTIGITTYAISRSFPKSALLNINLPSSRFQPMQFDHDIGVDGKGIGELYIAHSHYFNRKATLDYLRHLETLYLTRYPVLNFSDIPGIDATCSLGFNLFTCIISLRHNPFIRSLKISGVPHINVISSIGEMMQTNTSITELEVTNLLTEQSFAPLGHALMINDRASALQILNLSNNLMSYESIISLCEGLANFNHSLTSLNLSKCNIPPRGIKLLFEAFEKNFAMSLTLEELNLSENRFQDSGTSAFSSWLSKSRGHNSLLKLYLSQTSLNMSIISPPLRILEKLCILDLSGNNINISSARLLAVETLECIPKLVFLNLSSCGLVGNSLEMILLALRRNSNIKNLNLNLSNNSFAAKTCQVLVKLLPDSHFIAGLDLSFNSFVTRQLIEILGSTSQLKSLVSLDIGNNPFSSNDEYLIPKVVEFMTQHPNIVKFGIGSADRQLGSTLHPLIQCLNFNKSIQVLNISGNGMNDHGACLLADCLRNNKSLKKIFIYGNQFTSVGWQSLASPFIFFRNTSVEHIDLPVFDQPINVSDSNMVPLSPIKRAQLQDTFAQIQMQLNLNKIKLSASQRFGFLPQDDPPIYVKPPATIPDHLSGIVNSTSAIQSAGVPSGSSTIIGKMEKITLSNTTKVNNVISSIFSKPINTLVNISNDIASLKSPTLHNHGNTNGGGNPELEQQPSRPRNKTPTFWNEDEWSNSNSTSNVKEGNDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZF8</accession>
    <name>A0A8J4PZF8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001818</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000046">
        <property type="protein sequence ID" value="KAF2076851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZF8"/>
    <dbReference type="OrthoDB" id="24429at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550:SF10">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="776" mass="91131" checksum="B77C12389019C4D8" modified="2022-05-25" version="1">MNKSKNNSTSNTDSSNNNNNSNNKNLSKQLFDLVFHNKFLSKKVFGYNKGDYRYKDIFSVDWIIDRGYFGLLKDKVKLNEKLHLKDPYCLFKIKDLQLFKLVFSRYSFYFVIEFPIQLYTAVQYQHYDIFKYLISLGYRGEFKKCYCVAWRDGQYQLVNYLLEIDHDIDRSLVLSNIIYSIKYKQKDTLSRLLDKYLLFNSINRNSSDDSNDGDDKGNRHGRHKTTSRSDEFDSIFDNSNNIDDERFYHYIYREVIGCGDIEIFKILNLYIPIRFDLDNWFRLGKENQNLVIYLLETYKGMISTQLIIDQLKTGSQHSYHRIKLVFYMLENSMISLKDYNLLRVEIVKSALVVHDWETVNQFRVKDEKMDFKSKILSDIDNWNSSLGRVKELHDLGVSIPETSVHQAIRDDNLEIYKYLCNKTVYRGGVNQHRKDVQRHRIAIVNRSAKILQFQMAQDNLNFDCDEIARSWSVADMNILALFALDQYPKIQAKYHELQCKLSIRQHNAACFKWNYSMVETRFPDKINMVSIEFYKEAIRESCVEILMFLIQEKKLPSLVENGVLVLAKTTQVVDILLEAGHVIKSSVYDALSTKRRLPNFNILEYVLSKSPNTTLNNIDTLLNISLCDKLPHHFHLLLRHHLLHTPPKLVTPSLITMISKEATWKHIEYFNQLTPNLDFSNSLFLACMESGNLDALEYLVRVNPSIPISVSKHSICNLFTVKPMVFSVLRNGMDPESFNEFIDRFTKANEFIEYVQLDKYLASLRYTPMLKKLKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q048</accession>
    <name>A0A8J4Q048_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">serine C-palmitoyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.50</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_001395</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-serine + hexadecanoyl-CoA + H(+) = 3-oxosphinganine + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:14761"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:58299"/>
            <dbReference type="EC" id="2.3.1.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.50" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000033">
        <property type="protein sequence ID" value="KAF2077271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q048"/>
    <dbReference type="OrthoDB" id="65434at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017059">
        <property type="term" value="C:serine palmitoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004758">
        <property type="term" value="F:serine C-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06454">
        <property type="entry name" value="KBL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF3">
        <property type="entry name" value="LD36009P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="9">
        <location>
            <begin position="109"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2077271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="493" mass="55066" checksum="29A636D6DE1BA8B3" modified="2022-05-25" version="1">MEEKEVDSSEDVGIIPVIFLYFAYAYVILWGKIVELISKIKGERYFTAPEGYAPLFVEFEYFYQRHLFGRIKDAWDRPICSIAGAWIDVMNRSNNHYQERLQLTGGSRKSLNLGSYNYLGFAQNHGPVTDKVLESIKNYGVYSCSNSAEVGYTGLQKQLEDLTAKFLSKEACMVFEMGFATNSGTLPALIGKGGLIISDSLNHASLATGCKNTGCKVKVFRHNDHQHLEEVIRESIIQGQPKSHRPWTMILIIVEGIYSMEGEMCCLPEIVAIKNKYKCYLYVDEAHSIGALGKTGRGVIDHFGIPSSEIDILMGTYTKSFGGIGGYVCSSKALIDHLRQSSFSCLYANSMAPGCLVQAYEALRCIMGEDGTTIGKQKIKQLHDNSNYFRTRLNEMGFVTLGSKDSPVIPLMLFNPGKMSSFSRLCLARHIAVVVVGYPATPLSSPRTRFCISSSHTIDDLEWALQHIQEVGDLIGVRYFKNKHQNQKHIKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q184</accession>
    <name>A0A8J4Q184_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002691</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000077">
        <property type="protein sequence ID" value="KAF2075984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q184"/>
    <dbReference type="OrthoDB" id="20491at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF173">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="729" mass="80412" checksum="ADDB9E9DA2F775DA" modified="2022-05-25" version="1">MNKLFEITRKDSGSHKGGVVDDSLQIQRFTFQTKPSSSSFDLQNCINSSKYNSRSNCKQGDNDKLFFMVWRNKYLNRKIFGYLNSDNNCISIIYSKNKPLKKKSIPTLFENVHLILKNNRTISYIPPCVSKLTICSEFRCSLNDCIFPNTIKKLSIESCIDRPLQAGILPNSISHLSILCKYGYSTQVLLPSGAIPDSVSHLTISTTSTSNNPLNPVLEVGSIPSSVTNLKFGCYFIQDLVENLIPNSVNTLIMGQNFNQQLAPGILPKSITNLSFGFFFNKSLEPGTIPSSVETLVLGDCFNKALLPGSIPSSVTHLTLGSCFNQPLTGCIPQSVKVLILGRDFNQTLQKGDIPPSVKYLSLGIEFNKYIAKGVLPNSITKLVMSYKYNQVLSVGSVPNSVIHLTLGACFNRALLIGSVPRSVRNLELGFHFNQPMDTHILPKHLKSIKFGFCFNRAIKVGTIPQGVREVEFGFCFNQPIEKGSLPNSITSLKLGCCFEQPIYTGMIPNSVRFLSIGDPCVGSMGKCLISKNSIPESVTNLCLGQIPKNPWERGIIPSSVTHLAIKCYNKSIGEPFIPNSVTNIVWYGDSPMKGIIPDSVKHLVIDCFGSKILKGTIPESVEYLKLCEGFNQTISKGLLPSSLNYLHLNEYFSKRIEKGSIPQSVIKLSLGNASICRSNTIPKSLLSLKLGKKYSSGEKHIKKRWIPQSTQSLSIPPTPTYPTSVFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1T8</accession>
    <name>A0A8J4Q1T8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C-module-binding factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_002108</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000056">
        <property type="protein sequence ID" value="KAF2076557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1T8"/>
    <dbReference type="OrthoDB" id="16945at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018866">
        <property type="entry name" value="Znf-4CXXC_R1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10497">
        <property type="entry name" value="zf-4CXXC_R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="JmjC" evidence="7">
        <location>
            <begin position="140"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="PWWP" evidence="6">
        <location>
            <begin position="867"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="583"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="648"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="726"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="583"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="648"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="670"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="687"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="726"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="803"/>
            <end position="816"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2076557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="992" mass="114008" checksum="DB40528B53DFC9A3" modified="2022-05-25" version="1">MQVDTARYLEFPMFVPSVEDLLNFDIDHLINAPNSNFIEAASIPYTAATLETVQDFTINKGIPVVLTNTTNSWRTDIFSLNFLQDNYSSLPLVNSPRNNETHSDLEGWTLGEYINYLQVSPEERNPKYLYAKDIQIPKEWTSFLESNLFNFLTYKSQHDLISNLPGYLQPEGQMIYIGSNGTYTPGHLDLCGSNGNNLMVSSDPDSYSYWFIVPRQYKDVAINFWVEEGGDIYNESRFIRASDLLKAPFPIHVIKQKPGDFVMVPPESVHQVINVGGTSIKVAWNSISLKSIPISYYSSLPDSRRVSKIELFRIKATVYYTLKNYIHQIESGQLDSSTDIKHQIEMISPLLEIFHHTLQTESILIPKPNYPYCYGETIPFLQPFKYFNTSTIQDRRCDHCNCDIFNRCYHCPHCKTDSGGKDYCFDCVSTGIGCEYHFKDMTLKEFISHSKLKKDLAQYYQVFKNLLTQSGLRDREIEDIVRKRTDKVQEECGFLTTATVAYHVVYYSSQKKIKCHRCEKRYKKFSIVFCTNCSSRYCEQCVIHFGHNFPLLTRRSEWNCYACQGVCDCSTCLNNINNSNQIQNNSNQNNQNTFYNNNQNNQNNNNNNNNQNNSGSPQVLTISSVTTLPPILTSSTEKITYLDEKNNLNNLMKNNNNNNNNSSRDERHSNNNNNSLNNNQRKDSHYNNDNNNNNNNNHNHNNNNNQNLNSHEETCFEFLTTKLLNNPNNPNNLVNSHSHNNNNNNNNNNNNTTNNNNNNNNNNPNYFRTSTLTSLSTSLSSSAPSSTQKPMDIHSKKRPIVLDNDKPKGRPPKNLKEWTSTHKFIISLIEIFRNKNSLSSIPNNPNYKNNGQISHIVNMYLKNRDCFGGLLWAKTKKFPLLPCVWVKDLSVIPPATKLLPSLIQGKKIVVLFFGGLEDEEVAFVGKKSIFSFDEVNQTLLLKCNESPLRELEDLFHLTEPEIATKKEIQTFNYKNQIEEKEEGLFVKSEVYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2G4</accession>
    <name>A0A8J4Q2G4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001214</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000027">
        <property type="protein sequence ID" value="KAF2077515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2G4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008455">
        <property type="entry name" value="HssA/B-related"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05710">
        <property type="entry name" value="Coiled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="69" mass="7369" checksum="A4E716C3C260D299" modified="2022-05-25" version="1">MTIFGSITKLGSVKSNYKSNQITSSASSNQLAIAGNETAKEAHLFRLEVVFHALLGLLLGIHANAEILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2M7</accession>
    <name>A0A8J4Q2M7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001034</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NmrA-type oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000023">
        <property type="protein sequence ID" value="KAF2077647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2M7"/>
    <dbReference type="OrthoDB" id="3358371at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05251">
        <property type="entry name" value="NmrA_like_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051164">
        <property type="entry name" value="NmrA-like_oxidored"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42748">
        <property type="entry name" value="NITROGEN METABOLITE REPRESSION PROTEIN NMRA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42748:SF7">
        <property type="entry name" value="NMRA LIKE REDOX SENSOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="3">
        <location>
            <begin position="3"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="302" mass="33718" checksum="6DF4AC331D206237" modified="2022-05-25" version="1">MSKKLISVFGATGHQGGSVVNALLEKRDDFSIRIFTRNTNSDKSKQLKNRGVDVVELDLEKVNTMAITKALEGSYGVFIVTDFWSLGNKEIQITKNIVDAAFNAHVKHLVYSSLAHVSKITNGRICSPEYDNKATVEEYVRERYNNAKNTAMAVSFVQCPFYAQNFHSDAFKPLRDNNGVYRLRMPLDPALKPLEIGDISNIGYIVLGIFSNPLKYNLVVVPFAGSTLHGKEIAEIMSKTSGKKVEYQFVPTSEYDPPSIADMFRFCSEYGAFNGLDTSLATDITRLTTLEEYLTNNPLDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2S6</accession>
    <name>A0A8J4Q2S6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_000969</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000021">
        <property type="protein sequence ID" value="KAF2077722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2S6"/>
    <dbReference type="OrthoDB" id="2573941at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12411">
        <property type="entry name" value="RRM_ist3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051847">
        <property type="entry name" value="RNA_proc/Spliceosome_comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045844">
        <property type="entry name" value="RRM_Ist3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45880">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN, X-LINKED 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45880:SF1">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN, X-LINKED 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="33"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="111"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="174"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="237"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2077722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="338" mass="41012" checksum="D5D654A852400CF2" modified="2022-05-25" version="1">MNVVREIDRINQRELSANIGEKGSWHADYAHSPYIFVGGLSDELNEGDVMAIFSQYGEISECNLVRDKESGKSKGFAFIGYDDQKSTILAVDNFNGIKVLNRTLRVDHVKDYKKPKKNSDDEDTDSDHSSNTDQSSSEDDEQKRKRKERKAEKKKQRENETPEQRLERKEKKKEEKRKRKEEKKLKRLEKKRIREEKYKDDDDDYEKKKKPSLHDIDDRKDEYIKDRDKSYSGSNSSREKDYDRYDHRSKRDDDRYKRDDRYSSSRSNTRNEFKDERYSNSSSRYDRDRDDERYNSRYDRNRDRDRDDYGRYNRDNRDRYDNRDKERNNDKRSSSYRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q399</accession>
    <name>A0A8J4Q399_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_000762</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000015">
        <property type="protein sequence ID" value="KAF2077962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q399"/>
    <dbReference type="OrthoDB" id="16717at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000237">
        <property type="entry name" value="Flowering time control protein FY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001116">
        <property type="entry name" value="GM10830"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045245">
        <property type="entry name" value="Pfs2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22836:SF0">
        <property type="entry name" value="PRE-MRNA 3' END PROCESSING PROTEIN WDR33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22836">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="155"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="190"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="231"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="273"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="315"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="404"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="441"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="441"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="476"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="488"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="504"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="523"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="568"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2077962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="598" mass="66695" checksum="14E3CA808F29BB1F" modified="2022-05-25" version="1">MSHNYNRDHDGGEGGGGGGGRGGHHHGGGGRGGHHHGEGGDGGGGGGRGGHHHGGGERGGYHNHYYSNYHASKIVYDGKRMRKAVVRKTVDFNSTLIKYNQTKVFQRDYRDFSAVQPDSLYIREILPPLALDSNPITSICSRFIHTSANKVKYPINCVTWTPEGRRLITGSSSGEFTLWNGLTFNFETILQAHDTAVRAMVWSHNEDWMVSGDDGGLIKYWQPNMNNVKIFQAHKESVRGISFSPTDFKIASCSDDVTIKIWDFARCKEEKVITGHGWDVKCVSWHPQKSIIASGSKDNLIKMWDAKSGENITTLHGHKNTVLQVQWNQNGNWLVSASRDSLLKLFDIRTMKELQTFKAHKEVVKEMTSVAWHPQHEDLFVSGGFDGSILYWVVGQDNPQGEIYSAHEASVWSLSWHPIGHILASGSNDHTTKFWCRNRPADTMKDKYNNPHASKDIENEEDAPEVPDFILPGLSQPVTNTATSGGHSNTNTPPTANSPTLNAQQSSPTLASVNLYQPPQPHAQPQYSPPNNNNFNQFPQQSPPLNNLNQNFHPYKQPNNNINMNPMPQQPPQQLPPTFTPPPQMQKKNRKQQSNFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3J2</accession>
    <name>A0A8J4Q3J2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000489</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000009">
        <property type="protein sequence ID" value="KAF2078199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3J2"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000559">
        <property type="entry name" value="Calcium-binding EF-hand domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="3"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="39"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="73" mass="8327" checksum="FDF7A6CE510F489C" modified="2022-05-25" version="1">MADEKSQLIEAFYNFDGDYDGFVSIEEFRGILRDGLPMSEAEMKEFFETADPNNTGFIDYKAFAGMLYSVDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4B1</accession>
    <name>A0A8J4Q4B1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">General transcription factor IIH subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_000650</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the GTF2H2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000013">
        <property type="protein sequence ID" value="KAF2078012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4B1"/>
    <dbReference type="OrthoDB" id="284275at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01453">
        <property type="entry name" value="vWA_transcription_factor_IIH_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000015">
        <property type="entry name" value="General transcription factor IIH subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007198">
        <property type="entry name" value="Ssl1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004595">
        <property type="entry name" value="TFIIH_C1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012170">
        <property type="entry name" value="TFIIH_SSL1/p44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00622">
        <property type="entry name" value="ssl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12695">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12695:SF2">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07975">
        <property type="entry name" value="C1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04056">
        <property type="entry name" value="Ssl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015919">
        <property type="entry name" value="TFIIH_SSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01047">
        <property type="entry name" value="C1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="VWFA" evidence="16">
        <location>
            <begin position="86"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="371"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="12">
        <location>
            <begin position="303"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="51"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="409"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR015919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR015919-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2078012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="430" mass="48687" checksum="05990BC2AF928660" modified="2022-05-25" version="1">MNRKQNKSQNQSLYDDEDGPVHVLQTNDGDGTNKYKWENRFERPWDMIEEDEDGGLRPSIDEERNNRNRRLRKGENKRVRRGMQRHLCLVLDLSKTLANQDLKPSRYFVMLQSCELFIKEYFDQNPISQISLIITKNSKAEKITELSGNPSRHIQAMKDAIAMEGEPSIQNALDVAISNLSFVPKYGSREILFLFSSLTSCDPRDLAATIKTLKAENIRVSFIHLAAELYICKYISEQTNGTSKVILNEEHLQESLLENCQPPPTIGKVEASLVEMGFPQKQTLPFPSPCICHEKMRFSGYSCPKCSSKSCELPTECQICNLSLVSSPHLARSYHHLFPIPMFQEINWRTLSGDFSCYGCLIPPTSSSLYFQCPRCTQSFCLDCDLVIHESLHNCPGCENNLDNPPAVVDNSAGDTTTTTNTTNPIEINT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4V4</accession>
    <name>A0A8J4Q4V4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SNF7 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000386</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000007">
        <property type="protein sequence ID" value="KAF2078294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4V4"/>
    <dbReference type="OrthoDB" id="10266568at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10476">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2078294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="21804" checksum="367EAF9CDFCDEE8C" modified="2022-05-25" version="1">MENQLFQLKFTSKQLEKQSKKSESNEKVQKTKLKKAIEQGNMDGAKIYAQNAIREKNQSLNYLRLASRIDAVASRVETAIRMKAVTGSMANIVKSMEKSMRNMDLEKITQVMDQFERQFEDLDVQSVYVENAMNQTTTLSTPADQVDLLISQVADEHGLSLGMQMGAVPNEKVQQVETDELAERLNRLKQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q513</accession>
    <name>A0A8J4Q513_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000266</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000005">
        <property type="protein sequence ID" value="KAF2078399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q513"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040291">
        <property type="entry name" value="DDB_G0287341-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35202:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35202">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035162485" evidence="2">
        <location>
            <begin position="16"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="200" mass="23371" checksum="CBF8CD5A356B090D" modified="2022-05-25" version="1">MFFLLSISLIFASFGLPWYELDSVTKWYFRESSEKIEFKVFSVSNVYEIDKKYENYYGLNINQKNTILGSFICGLLSIIVLIFLIICYGVYNSVPNKTIFKHLIRFIPWLAFALTLISCLSFLRFQQAIYDDCEILKSDKYRFCRNPFGTWIKTEYYSNQVTNWGRKSGWSIVVAATVLNFVSSIIAFIVYIPSKNDNTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q529</accession>
    <name>A0A8J4Q529_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cysteine proteinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_000296</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000005">
        <property type="protein sequence ID" value="KAF2078429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q529"/>
    <dbReference type="OrthoDB" id="10259130at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.170:FF:000001">
        <property type="entry name" value="Cathepsin L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.170">
        <property type="entry name" value="Cysteine proteinases. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0458">Lysosome</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035230642" description="Cysteine proteinase" evidence="10">
        <location>
            <begin position="21"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="12">
        <location>
            <begin position="35"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="11">
        <location>
            <begin position="124"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2078429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="371" mass="41358" checksum="2E10C5BD16BA0A91" modified="2022-05-25" version="1">MLKICTIFLLVLLCTALASATYKSPFSEQEYRQAFTEWSLKHERTYDSGEFNQRYAIFKMNMDFIHDWNSQGSQTVLGLNKFADLTNLEYRKFYLGTKINTYDYGYDGRSTDPIFSSSSSEGKENNIVDWRKKGAVTSIKDQGQCGSCWSFSTTGSVEGAHQIKTGNLVALSEQNLMDCSRAEGNMGCNGGLMSFAFNYIIKNKGIDTEESYPYKGVDEKKCLYNVSNDGATISSFGNVTQGSELSLQTAVKNHGPVSVAIDASHNSFQLYTSGIYYESKCSSTQLDHGVLVVGYGTMSKTSKIRVNNRLSKVVPHQETQESSSSDDTTNNQYWIVKNSWGTTWGEQGYILMSKNRRNNCGIASQASYPII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q541</accession>
    <name>A0A8J4Q541_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000242</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000004">
        <property type="protein sequence ID" value="KAF2078492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q541"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1016" mass="114607" checksum="1E3DA96A74ED231B" modified="2022-05-25" version="1">MIDISFYLIWRNNFIRGLIRGKVLECIYIKVDLEYLNSNHQYLSLLSPLLKLNHRISVCLDVFNREQLVQYQNNKHKHLINYLCLDIGIHQRQRLGPIGSSDYDNDGDSGDGDVIDFDSLGRDIHTLVVYVDKSLRGVGKLPDSIYRLTFNIRRGYESVRCQFIDIILTDLPVSLKQLSLPNNYTVHVPVAIPDTLVKLYYCSTLGNLKQFVVSPCKTFDDCRLQVLSIDDLQWVNNQTWITSIEISGLDNQVFRPNTIAPHICWIQAKGQGHIFNVGSLPSGLKYLYLNCTDQLAKGTLPSSLKDLEIKRYNNPLELDILPPSLVNLSFDHYIHPIAPSVLPNSLAVLRLNTFNLPLPEGLLPNSLSELWMEIYSHPLTKGVLPPNLKVLALFTFNSTIAHGSLPNSLTNVQLPCYSAPLDSFGVLSNIRYLSIRYLNQSTLPVISSPNTKILVLSYRHRSNVSLASTFIQHLVLVNYGPKDYIDPSFLPPTLKSITTNGTDVSDINRNVSFMIDTTFYSIWRNVYIRGLVRVNVCREARIKVDLEYLNNNQQHLSLLSNNDKLENNISVILEITTIDQLREYLDNKYNSLVNDLYISIDGEKEQSGGLDLFIDCDRFPHGITSIEINVDRNARGRGRLPDSVTKVLLGRTDIDRFFTYPFIDHVLSSLPNKLEYLGLPLHIKINAPIHLPNTIQNVSYLSSQAYLQHFVVPPNKVFNDCTLEAKTTNDLQWISNNPWVTRINIPRIEGDVVGQNLIPKHLRQLTIYSDTEFEKGCLPPSLEFLSVGSTSELVDVLPPNLTQLTLGGYQGELTRGLLPSSLKLLEIFDFDNVIHLGAMPSRLVVLSLESFNQPLQVGILPNSLVHLRLNGYNHPLVPGILPSLLNTLSIAGFDSTIAPNSFPLSLTRLDLLSYTGSLDSIGVLDQLTDLSIVNFDRSLHRVISPKTKTLRLAYQNRSSDVDLLNTLIQHLVLVNDGQYNDDYKERSFLPPTLLTLKAYRIQITNIPNGCKASVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q5D6</accession>
    <name>A0A8J4Q5D6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carbohydrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000002">
        <property type="protein sequence ID" value="KAF2078624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q5D6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035301831" description="Carbohydrate binding domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="158" mass="18241" checksum="2366863392DFE7BB" modified="2022-05-25" version="1">MKLKLYAIVLLFLVVSPSFAQFLLFVDNHSNINVDIRVSRYEGKNGDGDDGVDTWWRGIPPGQSYPIIRAFPERGYIITVRTASYTDSWMVYSPNPPEYSLDEQGHLHIWNEHIQFRGAGINYTFKFNRNSHYFHTFTNSNHAPVCFGGETYGQIVWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q820</accession>
    <name>A0A8J4Q820_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">T-complex protein 1 subunit eta</fullName>
            <shortName evidence="9">TCP-1-eta</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">CCT-eta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_002296</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterooligomeric complex that forms two stacked rings.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000062">
        <property type="protein sequence ID" value="KAF2076391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q820"/>
    <dbReference type="OrthoDB" id="1935484at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03340">
        <property type="entry name" value="TCP1_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000022">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000006">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012720">
        <property type="entry name" value="Chap_CCT_eta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02345">
        <property type="entry name" value="chap_CCT_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="525"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2076391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="554" mass="60805" checksum="93DBC6A82765705F" modified="2022-05-25" version="1">MSQMIRPPIVLLKEGTDTSQGLPQLISNINACCAIVDTVRTTLGPRGMDKLVYQSEKNVTISNDGATVMKLLDIVHPAARTLVDIAKSQDSEVGDGTTSVVVMTGEFLKAAKPFLEEGIHPQIIIRAFRSACALAKQKIKELSIDIKPENMREFLEKCASTAMNSKLIASHKEFFSKMVVDAVQLLDDNIDLDMVGIKKESGGGMEDTQFIAGAAFKRTFFYAGFEQQPKKIVNPKVLCLNVELELKAEKDNAEIRITDPSKYQSLVNAEWKLFFDKLEAIHASGANVILSRLAIGDLATQFFADKKIFCAGRVPEDDLRRVCRATGAAIQNTITNIIPSVIGTCDLFEENQVGSSRYNLFTGCTKTQTATIILRGGGEQFIDEAERSLHDSIMIVRRARKHRSVVAGGGATEMEVSKFLRDFSLSIDGKKQLLINSYAKALECIPRQIADNAGFDSTDILNQLRQKHAQGEKWFGVDILNEGICDTYQSAVWEPSLVKLNSIVAATEATCLILSVDETVQNKAAEAPHAGPQMTQQSRQALARGRGVQAMRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q8H2</accession>
    <name>A0A8J4Q8H2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="7">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_001978</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="7">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="7"/>
    <dbReference type="EMBL" id="AJWJ01000051">
        <property type="protein sequence ID" value="KAF2076721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q8H2"/>
    <dbReference type="OrthoDB" id="422663at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17949">
        <property type="entry name" value="DEADc_DDX31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 7" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="12">
        <location>
            <begin position="174"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="206"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="418"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="507"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="799"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="480"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="6">
        <location>
            <begin position="174"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="24"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="123"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="512"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2076721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="843" mass="95887" checksum="517D1D5E57BA154A" modified="2022-05-25" version="1">MADDGLQLNIVYLNNAPKPPPRPVNNNNNNRGGNNRFNPRDKQDSNNRGGYDRNNSNSNNRFNKPQQQEKKKPLTYLEKRKIQEEKQKQLKQNKPAQHHRYQKPEDNADDGNDSSGTRQTQQQEKEREEENKTEIIKEKYREAEQVVEKDKFDRPVLPTFESKKLESSEVFSSMAFGSLSLSETLVKNLQGHMKLEKPTHIQEASIPPIMNGHDAMVKAETGSGKTLSYLIPIVQKLTEQRVNRADGCYSIVISPTRELCTQIFEVLQKLLKPFYWIVPGIIMGGEKRDSEKARLRKGINLLVATPGRLLDHLQHTKSFPTHNIRWLVLDEADRLLDLGFEKDVTSIINLLDEKKLRSSVRQNILVSATLNEGISRLASLSLHNPVYIGLDRAALDPASNPFNAATHAEMEMLHAPKQLEQYFVEVESKERLTSLVSFIKWKTAKTDQNKKEQASSKMIVFFSSCDSVDFHHYLFSTLKIDKERANKKRAKTNKKKLQQQKIQERLKALSEGNDDDNSDDDSGDEIFHSEDDSKTGLFNVPIFKLHGELDQQTRTKTFLEFKKSPNGILLTTDVSARGLDLPQVSWIIQYDACSDTKDYVHRIGRTARLGSSGCSLMFLLPSERDYVKHLNSFNLDIKEMKVTSILQSLFHTSEGQLKKTSKSSQLESLVHDLQIIFERFIIYDAKAKDMARAAYQSSLRAYATHKSDVKHIFHISHLHLGHVSKSFALRETPTELNKMSSGIKGAKAAKKGDEATLTKQTIEHKMKNYKSTSEFSDGLAGGVVKDKFAGTLLNIFKKPFKPREEGDESNGNNNSSNSKKRSFDNNKRSSSNSFDKNKKFKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q9M9</accession>
    <name>A0A8J4Q9M9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BOS complex subunit TMEM147</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Transmembrane protein 147</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_001244</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TMEM147 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000028">
        <property type="protein sequence ID" value="KAF2077471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q9M9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019164">
        <property type="entry name" value="TMEM147"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12869:SF0">
        <property type="entry name" value="BOS COMPLEX SUBUNIT TMEM147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12869">
        <property type="entry name" value="SMALL SEVEN TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09767">
        <property type="entry name" value="DUF2053"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="77"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="153"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2077471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="256" mass="29160" checksum="F715B4DF3C1CFD4B" modified="2022-05-25" version="1">MALLYLTSCLLLTFGPYHVIYYSSDLNKYHSKPIITYGFLGNSITQLFKTILYSTFSFLSSLQIPILIDILFLNKEFITILSLIIDIAGVWITLERAFKISNNRGPIHIICVGLGWSLFSSMTYRLKPIVFGTYGQSFNWSFALQGIEANLDIFKYIVIALFLYLMALLAEKNHKTQSNHKTRIIKSYDKNRIIVYLCLVSYILFPALLYDCSSCSGGNRDTPSSLSSVVLNIIHSVSIATLIKRNQYFNTFFKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4QAM7</accession>
    <name>A0A8J4QAM7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquinone biosynthesis O-methyltransferase, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">3-demethylubiquinol 3-O-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.64</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">3-demethylubiquinone 3-O-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Polyprenyldihydroxybenzoate methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.114</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">coq3</name>
        <name evidence="7" type="ORF">CYY_000607</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">O-methyltransferase required for two non-consecutive steps during ubiquinone biosynthesis. Catalyzes the 2 O-methylation of 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoic acid into 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoic acid. Also catalyzes the last step of ubiquinone biosynthesis by mediating methylation of 3-demethylubiquinone into ubiquinone. Also able to mediate the methylation of 3-demethylubiquinol into ubiquinol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoate + S-adenosyl-L-methionine = a 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoate + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44452"/>
            <dbReference type="Rhea" id="RHEA-COMP:10930"/>
            <dbReference type="Rhea" id="RHEA-COMP:10931"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64694"/>
            <dbReference type="ChEBI" id="CHEBI:84443"/>
            <dbReference type="EC" id="2.1.1.114"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3-demethylubiquinol + S-adenosyl-L-methionine = a ubiquinol + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44380"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="Rhea" id="RHEA-COMP:10914"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:84422"/>
            <dbReference type="EC" id="2.1.1.64"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3-demethylubiquinone + S-adenosyl-L-methionine = a ubiquinone + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:81215"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:19654"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:231825"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of a multi-subunit COQ enzyme complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Mitochondrion inner membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. UbiG/COQ3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.64" evidence="5"/>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="EC" id="2.1.1.114" evidence="5"/>
    <dbReference type="EMBL" id="AJWJ01000012">
        <property type="protein sequence ID" value="KAF2078056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4QAM7"/>
    <dbReference type="OrthoDB" id="3265906at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061542">
        <property type="term" value="F:3-demethylubiquinol 3-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010420">
        <property type="term" value="F:polyprenyldihydroxybenzoate methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00472">
        <property type="entry name" value="UbiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010233">
        <property type="entry name" value="UbiG_MeTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01983">
        <property type="entry name" value="UbiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43464">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43464:SF19">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS O-METHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 5" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="6">
        <location>
            <begin position="122"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2078056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="312" mass="35002" checksum="F8FBD8BB1C602CDA" modified="2022-05-25" version="1">MQQSIKALSKLHRFTATTTAASSIKCWSHSQPLCRSFSSTNTTPNDNININNINSNNNTLKNEEIQFFNNLSKDWWNPEGTMKPLHRMNPLRVQYIVDSIKSSTSLISDNPIQYPLKGLKMIDIGCGAGLLSESLSRLGADKVIGLDAAKNNVLMAKSHASADIDLKENIETNKLEYVESTIERFIQDESMVESFDVVCSLEVIEHVDNPQEFLKLCTRLLKPGGHIFISTINKTPTSFLATILGAEYIFRMVPIGTHHWSQFIKPADIHSALAQNNCAVKDTQGLFYNPYDSSWSLIKDLSVNYILYAIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQS0</accession>
    <name>A0A8J4UQS0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab3-GAP regulatory subunit N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007632</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000418">
        <property type="protein sequence ID" value="KAF2071056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQS0"/>
    <dbReference type="OrthoDB" id="360390at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026059">
        <property type="entry name" value="Rab3GAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032839">
        <property type="entry name" value="RAB3GAP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12472:SF0">
        <property type="entry name" value="RAB3 GTPASE-ACTIVATING PROTEIN NON-CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12472">
        <property type="entry name" value="RAB3-GAP REGULATORY DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14655">
        <property type="entry name" value="RAB3GAP2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab3-GAP regulatory subunit N-terminal" evidence="2">
        <location>
            <begin position="110"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="479"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="91"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="480"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="608" mass="67866" checksum="4227FA0F60241763" modified="2022-05-25" version="1">MDDFVFKLASIGSLDIESIRLVLNHNETTTTATTEDIDNSDDNDHDNNDDENDDNDDDDDEYQSASEDEDHDKSSRKQQQQQQQIISPTLSNTSNTSSSSNSTAVNDELWLNKMLVDITSDGQYMIIGGGGNGSIGTGLAIILQKRQQQQQQQQQQQQQQQRSNSNSWRTVVTLDHQTQDGVDRITQVAFVPIGSPQIHYVAAVGYTSGYLRIFSMQGNLLLSQLFYNKPILKIKVKNTPTTPLEGNDQQRFHIQELTILYPDNIVANISGVNLVTIVQVCYNQVLRGDSGGSGNNSLSYRKWTLADSVEKVNDITFCGPFLGSPFRALPYSTTEQAMIRLVGVGKDPMISFYYPSEDNGVSFSVMISSITSRLTTAVYSFAKNWWSGGSSSSSNSTASQQSQQTIEKPVPLTLRWCITDQKREITNIVLDPSGRYAAATDNLGRVLLVDLVNSLIVKIWKGYRDSQCGFINVLEEDDNQSNTSLNNNDSNNNHNNNNDNDEDIEDFIPKKLLKKPSSFQSRTYLAIYVGRRGLLEVWGLKHKSREYFKMVGKGCKLISSNIPSSINNNLNNHNNNNNSSNGTNYQAGQSHAFILYPNGNIQQIQYQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UR38</accession>
    <name>A0A8J4UR38_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_007118</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Rho GTPase-activating protein involved in the signal transduction pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000362">
        <property type="protein sequence ID" value="KAF2071566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UR38"/>
    <dbReference type="OrthoDB" id="19923at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050729">
        <property type="entry name" value="Rho-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176:SF12">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN GACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176">
        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="7">
        <location>
            <begin position="86"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="322"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="597"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="649"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="888"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="682"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="764"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="331"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="352"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="391"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="597"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="618"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="652"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="663"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2071566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="911" mass="104439" checksum="8248EFF8ACCB58FA" modified="2022-05-25" version="1">MKNIFRRSVQIFNKKDKDEKGGVPGSPSTGKKDKKKDKNGVGDSSSNDSPTLSRVESVSSYNSDSISSATGPVFIYNPNAKKVFGVPLTQVPCRADSNVPIIVEKLVDHLEKTALTTEGVFRIPGRDVTINSFVKLFDNGEDVDVSPIEPYTAAGLLKRFFRDLPVFVPQQINKRVASLFVSEDGKKKPIDMEILGKLRYLVHQLPSVHFEVMQSLTGLLSKLMSKSDENKMTISNIAICLVPTLNCVPAIVTYPIQYYDFFFNEIFPEHHLYHMRPYQTNLISTAIEEKLEHLSTQPMLIPNHDMQKRYSRPASITISSLVPNVAPNNQSPSSSTITSTTFTSPHHDYPSTPITFTVTTTFTPSLEEQQQQQQQQQLQQQQQQQQQHYPTDTKSERRGYKFDPNDLQFMDTSSDNNSQYLSNKNYLSTNNTGTMNSISSLSSLDRSNYRRSVAYNGTYNSNEDTKAAIQQIKEKIDRYSKEKPATRTREDKEREKLLRYSVDFERFKERYIKEKSDRSSRDINKEIEREIEKRLSPRERLNLLLQSSGGGTSASSTITSRDSKNYQRYSMNQQLQQQLLQLQQQQLQHQLHQPNLSASLSNLNPPPTTTTTTSTTSANKRYSSNNYRYSGRQDYMDEEFYSNFNNSLDMNQQQQQQQPQQQSMRDTLSRQRSQSCFEPENLLLQQQLYQHQQQQLQQQQQQFHHPSYNPQYSAAVIEQKLDKIRDTINNMNMDKDNRLSRDYTYYLREVEDLRSSLAKESVNAEFFVNKNQELEDRLRREEEKNQRLMEEIQLLETYFILKEKAKLSKRISTTKDILTRSRSPTLPTSMNPMMGGTGSLNSSTSSMFLSPPMNSNFSTSPSSSKEAIVNQMLRGSTRSLVNLMPTTTTTTTTTNTMPQQTSNTTPTTTTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UTT9</accession>
    <name>A0A8J4UTT9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010458</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001126">
        <property type="protein sequence ID" value="KAF2068218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UTT9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="175" mass="20171" checksum="D492145F62BBB60B" modified="2022-05-25" version="1">MILVYNDNMGISSMKFTLNTGTFCSKLSIYNLFSSRRYEMELVGNQYKIIKSPPNNLAFTSWCNANVFLQVGIVGSIKRHSFFMEANTWTKSLDDVPYLGDIKAIRVRAFGAQTQCNKFRITQTCPEGQSKCLPKNSPYRPLQGYRKTFDSKLENMNIGQQYLYIPFYPFGQVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UUF5</accession>
    <name>A0A8J4UUF5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidoglycan binding-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_003250</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000100">
        <property type="protein sequence ID" value="KAF2075421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UUF5"/>
    <dbReference type="OrthoDB" id="14788at2759"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16913">
        <property type="entry name" value="YkuD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.440.10">
        <property type="entry name" value="L,D-transpeptidase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005490">
        <property type="entry name" value="LD_TPept_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038063">
        <property type="entry name" value="Transpep_catalytic_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141523">
        <property type="entry name" value="L,D-transpeptidase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0133">Cell shape</keyword>
    <keyword evidence="5" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="4" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035283012" description="Peptidoglycan binding-like domain-containing protein" evidence="6">
        <location>
            <begin position="24"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="32602" checksum="BA7513B6C59B08FA" modified="2022-05-25" version="1">MLKLNQLTLLFVCFAALCAVIVADSSSSDEAYPCPFYRPLTVTNPPLSGSDVYILQNFLTRNPLFLDLQLTSQFDEATSAALAKFQTMNSIVTASPGDLDLNTANQLLEICSDDGYVDNGTIPPGFKYKVFVPVYRNRSIETTATLYDSNMNTLLQFTVRTHGQNVNETGEAQNQFCNNGYTPTGLMTFDLNSPEPDPVSFGPYPVNRAVQGIAGNAFIVISNIRDGILMHTGEWPGWNPSLPMPNSHGCIHGHPTDIDTVQQILTTKLGVGIRENMYTAVPYLHQPQGILSIQLMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXG6</accession>
    <name>A0A8J4UXG6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pyruvate dehydrogenase E1 component subunit beta</fullName>
            <ecNumber evidence="5">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_007771</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + pyruvate + H(+) = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="5"/>
    <dbReference type="EMBL" id="AJWJ01000433">
        <property type="protein sequence ID" value="KAF2070905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXG6"/>
    <dbReference type="OrthoDB" id="10266385at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07036">
        <property type="entry name" value="TPP_PYR_E1-PDHc-beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000001">
        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000001">
        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027110">
        <property type="entry name" value="PDHB_mito-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006667">
        <property type="entry name" value="PRK09212.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008854">
        <property type="entry name" value="PRK11892.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11624">
        <property type="entry name" value="DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11624:SF96">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 5" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="6">
        <location>
            <begin position="32"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2070905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="359" mass="39552" checksum="E27FDA44E8B45BD7" modified="2022-05-25" version="1">MFTLLLKKLNQPALNSTSKWVARSFSTAPKEVTVREAINSALDEELARDQRVYVMGEEVAQYNGAYKITKGLFEKYGADRIIDTPITEAGFAGIGVGSAMAGLRPVVEFMTWNFAMQAIDHIVNSSAKTHYMSGGKVFNPIVWRGPNGPPTSVGAQHSQCFAAWYGNVPGLKVIVPWSAEDHRGLLKSAIRDDNPVVCLESELLYNYKFTLSPESQDKDFLIPIGKAKVERQGTDITIVSFSRIVSNCLEAAEILAKEGISAEVINLRSIRPLDVETIVKSVQKTNRLVTAEEGWPQHGVGSEIAAQVVENAFDYLDAPIERITGADVPMPYAKNLEDAAMVQVQNIVNACRRVCYRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXT5</accession>
    <name>A0A8J4UXT5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_007237</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000374">
        <property type="protein sequence ID" value="KAF2071445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXT5"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03753">
        <property type="entry name" value="proteasome_alpha_type_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000015">
        <property type="entry name" value="Proteasome subunit alpha type-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033812">
        <property type="entry name" value="Proteasome_alpha_type_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 5 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="7">
        <location>
            <begin position="8"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2071445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="242" mass="26752" checksum="A101F84778C09E06" modified="2022-05-25" version="1">MFLTRSEYDRHVNTFSPEGRLFQVEYALEAIKLGSTTIGIQCAEGVVLAVEKRLTSPLLEPSSIQKVVEIDSHLICALSGLTADSRTIIDHARVETQNHRFNYDEPMTVESTVQSICDLALRFGENRKGEEKMSRPFGVALLIAGVDEKGAQLYYSDPSGTYTQFHAKAIGAGSEGAQQTLQEKYSKSMTLDECQALALTTLKQVMEEAVTTSNVELAKITTKDGKYKIYSKEELEVIVSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UY26</accession>
    <name>A0A8J4UY26_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006755</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000325">
        <property type="protein sequence ID" value="KAF2071940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UY26"/>
    <dbReference type="OrthoDB" id="155976at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016790">
        <property type="term" value="F:thiolester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247:SF45">
        <property type="entry name" value="PALMITOYL-PROTEIN THIOESTERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247">
        <property type="entry name" value="PALMITOYL-PROTEIN THIOESTERASE/DOLICHYLDIPHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02089">
        <property type="entry name" value="Palm_thioest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035291614" evidence="2">
        <location>
            <begin position="22"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="281" mass="32218" checksum="5CC8DEE48E99A63A" modified="2022-05-25" version="1">MNYRFANIVLSLCLFIVGTHSLKPVLLMHGFALSTTAGTYHDWNHYVSWLNQSNPDQIIYDVNQLIQGIIANNDNFSQGFHIVAHSQGALLMRSVIEMYGLNVDTFVSLAGVHNGIYGLGPLDQYPWLENLTDFELTEIFYTPTMQQGFSVANWWNCAVSRDRYLENNVFLPMINQEIENNTIANYKTNFLASIKGSLHAFGSPQDGIVTPWNSELFGFFDDQLFMKSMNETYSYINDSYGLRTLDSQGKLFLYQIDNVKHAEWLSRQDLFEQYVLPLISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UY79</accession>
    <name>A0A8J4UY79_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006424</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000295">
        <property type="protein sequence ID" value="KAF2072270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UY79"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="493"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="500"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="516" mass="59878" checksum="BA15F06D79E658E2" modified="2022-05-25" version="1">MIFNRINKIQKCIISVNKFNIYSTTARSLSTSTANCGCSNRSISTSTSLPTTTIQKRYFGSYTRNEDPSTGRNSNIVTKDAYFDTRRKKPLGTVQSYTFFEIDENLPTQEKEKKQIEMGWVEPSKLEGTYSQSHQQLAELYKEGKYDMVVHTFLRMEPTYMTHECIEMALDSYTKLYESFQSKAPVSAAPLVINKLIVKEILNTASVKQYPVEKFVDFCKALHIKPTADHIEKIFLTSFVNQILWVEKFLSFFPEEKKELIPMGSDAHSKVLYSLATNNQQAEAIRLFKELPHDVKCSQSMLRIMVNVFIDTDRKEDAKELLLRHFTTKTVDDAYVTTAINLCLLLGEIKLAAYFLIYMNKKNVFTHHELIQNVAMELGRRHEYALLDQLAKVIEVHHSDSLPSADYLLLMVNAYKGHPVTQQYYIKRLKAAKKTMVKNHQMVARGKYQLFVGLEHEFDLVQFSHDKDNVQSFSEHELEPQMEKTTKYFNKLSNNYGSGGHDREYIKHRNRDRYRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYC5</accession>
    <name>A0A8J4UYC5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006952</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000344">
        <property type="protein sequence ID" value="KAF2071728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYC5"/>
    <dbReference type="OrthoDB" id="10633491at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="182" mass="21826" checksum="2612B3B27177168B" modified="2022-05-25" version="1">MSYYSYNVNNKFASPIPLPNNIFNAIQNGHQISHPFFHRDCNLNNNNNNNNNSFSNHNYYNTPSPYYNNDQYSNYYNNNYDPYSNQYYSYPYYQQQTYNQGNEQWEEEEEEEEQEDEQEEEQEHYDQSSHSLYTLSDEVLEMFAKSEKKRREKQSAKKESKKLVVQDIFTPGESLPNNSSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYR4</accession>
    <name>A0A8J4UYR4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005218</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000202">
        <property type="protein sequence ID" value="KAF2073470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYR4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="733" mass="87251" checksum="75D1FEADA36ACA7D" modified="2022-05-25" version="1">MDSDCQQQCTFITLYRNTFLKSKIFSYLLASRKGYYVFNDSKLSLNYHQLNTLDLIRLNNISLLKEKYNRFGLVLNNNNNSNSNSFTCYQDWLDIDQSELYSTFQDNYDEDEIDEISLTIMNLIKRQDEKAAFVKSLLDRFGLTRNRLQIFNKYIPDKVYHPSDLELFGELVAENRIEIDYYSSLVEDQFFRENKDLFRGISDWTDEAFLMNLFSSGFDYEIHLPLLKWILTVSPLWNYDRYMQEKEKEKGDDLETTLVEYVYLYDIKIVYDRDDPFYHVLRCKNYDYAIASTEILQYTIDNYPEDFKKSLYEFDYGRMIDDPNTIRLLLEFNNFVALPFSIYTKSQDFDIIKFCVQHQQEIDGPNLMDTLEKLQYVVEHLRHIFDSHFEDCLILSIGQNHLEYMKYCLDHAPDLDGDHYFGNIFQVASKADQLTSDTFKYFCQWLLTTNNSRGESFDDVEFNLCLQLIKSNRTTFDQFKECYENTRIPKSLIPDLLFAAYGYLKESKSNKKQDAIKILEYLKSQHQLHLVPSSQECYSAQYFNAFIQDKEYVQYLSSPSTDLCWYIKGAFKIGSVALLERIVESMDKSQYLNFITRVEDILLAYFYTYPHLMPTQFKVPLYCFERFADQFSQSTLTTLSQFALIRDNHTLIDILLHRTKVRLIRPQSPEHDKQMLELNYMNLIKSQYFSRTNYFKLYPFTLDIDEKRLKTHKVRPFGKPKEGFDNLYLNIAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0P0</accession>
    <name>A0A8J4V0P0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_004062</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000137">
        <property type="protein sequence ID" value="KAF2074623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0P0"/>
    <dbReference type="OrthoDB" id="16596at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861:SF1">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="3">
        <location>
            <begin position="51"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2074623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="252" mass="28965" checksum="A47320CDE02E1727" modified="2022-05-25" version="1">MDTSEFVSQNIYDRENFLTHYFEKFERSKEGDDCWEIMLNHLPKSVKGMDILEIGCGMGFVANYLAEEGAASVTGYDISESMIELAAKGNAHNKNVKVGVQDMETIELPEQQYDLVISLYAVHYVLNYKRLIEIVSKSLKPGGKFVLFVEHPVFTSSIEQPIWIERPSGNKAWTVSNYFTEGKRFTKWCSFENVQKVHRTVSTYIQTVLEQGLTLTHLEEFCPTPQQMLKKPEHVNNFNSPLNLNMTFTKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1X9</accession>
    <name>A0A8J4V1X9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_010260</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001002">
        <property type="protein sequence ID" value="KAF2068414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1X9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057709">
        <property type="entry name" value="DUF7949"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25820">
        <property type="entry name" value="DUF7949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035304834" description="EGF-like domain-containing protein" evidence="3">
        <location>
            <begin position="23"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="1019"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="995"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="1043"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1065"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1065" mass="118875" checksum="778B4850F90C8E5F" modified="2022-05-25" version="1" fragment="single">MNTKIKLLYVLLILLSWNLVQGKLDLLSCELGKEQLTQVGCSLDIFISILSDEVISIDSSSSISPLSISIQERYGPYIAKLFAVRGSYSFGPKNLSVNAKYANGTLVEVLLGKEYDCLPLTAPSLSYYATPLFPTQKNWLFFYLLNIQRSLQLSVVWISPGYSSLIYQNPYYLSSFSLLIFASPNVSNSSSAQFNLMIGNYQIGPTMSIPITFSNYGELVSSSYSGVDGFYNPKRPFLYILAGLESLDPRNLWYQVSDVDVFRDYLKGSPMASSSFLISVPYLTQTSTLVRLSVYNGETPANIMNITGNNVPLNPVVELVLTQFINSQIDCKFIRVLFDLAYDRQDISLTGVYYRDKGNANIPFPFGIVKGTFKEPKYDISFSMSPYYSNANLFISVNEIFQPVNVVSSLSDIVPPIIHNLTLTHVSGPNYLLKATITDDMSGFNFLEINNRVVLTSADIVQGSLTNGTFEALVDQNYLLIADDISVYDNTLSFNRFPSGYFNSKGMKLNRIDSNLGVLILGKISSFAFKYFDLDLSTTGKWNTLNFNYTGAGPYSRIIFYPIISVSEYSSLDPDMKVNPKYQYEWDLNLKMFKIDFYLPRGLYTRNITYFIGDLSSDTLISSYEIFNVYGLHALLSVYSQEANQMFPYITTFDIKGSRIRNLELASDIVTIGWTFSIETLLHPLKYIILTVTSDYDPQGKNYTLTPSDSLNNDANLGDYELLFQVSGESVSQTYEISYALLEDESSRQSGYNQLYLQNAFFKFINTSSYNIQVKSPVDVIPDTTSPYLSFWSVPIESSYFYALEFDRAFEIDFVTKDADSKISAAHIPIVYLSPVISNARYACNTTLLALQDNGQTGIYKAFCNPEYGFGLPYGFSLSIYGIVDSNMNVRGYSGSQLSLESMPNYVKVSPVITFSPILEKITFNGTGALVLNGKKFGNSSLIYILEEKSSVWTLLNIQSFNYYTSSEMSFNYQTLSYFKIRIATLEGGNSNILAYGPSSGSNEEDSSSQGPPTPKPTTKPSCRNNCGGASRGTCLETGGCKCIGNYYGEDCTSEIIIIPTPSID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V264</accession>
    <name>A0A8J4V264_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000949">
        <property type="protein sequence ID" value="KAF2068519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V264"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="444" mass="50491" checksum="395187E2242A220D" modified="2022-05-25" version="1">MSNFMLNNPDYMPRAIRGARIIGTATDAVKKFKEHHSRKGYNYNPYDDNTVNIEPTSAPMFMTTNWSKVNYQACNYSYIKLMEEQASADLNPIYDNIKKNGDAAWRQVSQDGQDYWMSWGPSGFVWTYTDPSSMNKIYTSNSDSDPKVQMVVQFGTYSQSAQIAGIHSYNLTTKTLLGESVIALIVAKCLSGFIADGLDFLVTAFASRLAAAALEIGLDLTFALPEFVLPLVATCIVFTVVFIGLSYLFDWLDRKYTIRLQMFNWDDKYEWDCVNQYMSNALIPGHDDGILRFNLPKMVQPGTVIVPPGFDITTLDSICYYAMVIWNNDFTFMQGCSFSVNMSRNYGKYDPYHVDDGFTWAFNCPRWSNNQQALQDGSMDPEQYLNRVKKNNEWDPYPLKNSKVVTNDNTLVNFSVDYLEGAKDNLYNVLININAPRPNGNPNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2B0</accession>
    <name>A0A8J4V2B0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000519</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000010">
        <property type="protein sequence ID" value="KAF2078135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2B0"/>
    <dbReference type="OrthoDB" id="19393at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13671">
        <property type="entry name" value="AAA_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2078135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="289" mass="33353" checksum="3D773A6FA03974AD" modified="2022-05-25" version="1">MISCLTNTGINLISKPFSKCLPISTTTSYRYYTKREHDDVEKQWFKPKESLSSELERSNKESKPLVVFLEGPSGSGKRQLLERLSNTGYHCIIKPFIDVAKDNAKDILQPSSSFDIKNQSKLYDQLIKDNLNQSLLNSSNKSKDQAAFKNNLLFVHRSPISSIIYDKGNNQIESINKFIQQQKQQEKDYNVVYVYCKTPVELIQQRLGGRYHFNEDQDNQLVSKLNITDLQEFSEELYSTMEKESVFDEVLDTTSIKQACPSLLAMFNIYFNNDSLQQLIKDRLNGNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2I4</accession>
    <name>A0A8J4V2I4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_000344</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000006">
        <property type="protein sequence ID" value="KAF2078360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2I4"/>
    <dbReference type="OrthoDB" id="411372at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16464">
        <property type="entry name" value="RING-H2_Pirh2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039512">
        <property type="entry name" value="RCHY1_zinc-ribbon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008913">
        <property type="entry name" value="Znf_CHY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037274">
        <property type="entry name" value="Znf_CHY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017921">
        <property type="entry name" value="Znf_CTCHY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037275">
        <property type="entry name" value="Znf_CTCHY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21319:SF0">
        <property type="entry name" value="AND RING FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G08900)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21319">
        <property type="entry name" value="RING FINGER AND CHY ZINC FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05495">
        <property type="entry name" value="zf-CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14599">
        <property type="entry name" value="zinc_ribbon_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161219">
        <property type="entry name" value="CHY zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161245">
        <property type="entry name" value="Zinc hairpin stack"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51266">
        <property type="entry name" value="ZF_CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51270">
        <property type="entry name" value="ZF_CTCHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHY-type" evidence="7">
        <location>
            <begin position="18"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="CTCHY-type" evidence="8">
        <location>
            <begin position="90"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="155"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="281"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="287"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="317"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2078360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="354" mass="40618" checksum="72C2BFBA91A80B23" modified="2022-05-25" version="1">MDISNISEEELKETYFDVDKDILGCKHYPRACKIKAACCGKFFTCRFCHNEKENKHDIDRHATKFILCMKCKIPQLVSNQCINPRCETIFARYYCFVCKFYDDTPNKKIFHCQDCGICRVGSRDDFKHCSRCNMCLGVNTFDSHKCMEGTYLNNCPVCMEDLFSSRDSTVPLRCGHPLHFECYNQFIKANHYQCPVCKKSIANLANAWKRYDRLIESSPMPAMYKGSTASITCNDCHVKTMDMPLHFIGLKCSGCGGYNTTVMSQNVQIDQTLANDPNQSIFRVNEGDDVDQVSDDEEDDEDDDDENQDGVYGVGEYEGDEDDDEELDYDDDDDDDNEDEEFDSAEADEHNENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2K8</accession>
    <name>A0A8J4V2K8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009813</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000816">
        <property type="protein sequence ID" value="KAF2068864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2K8"/>
    <dbReference type="OrthoDB" id="21484at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="417"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="559"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="672"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="721"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="124"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="195"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="435"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="445"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="459"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="494"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="560"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="617"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="672"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="685"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="725"/>
            <end position="751"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="907" mass="98874" checksum="A1B88EDB36C152D3" modified="2022-05-25" version="1">MNNNNNSSNSNTSIGSNNNNSSSSSKSNINNTTFNNNSNSNNTLNNSSSNSNNSLHHIIFAATNSSFDLSPSPQKNTISSLTKPSSSSSSTTTTTAEKSNDLSQGFDQLKKRLLEERFDLPSQFSNKSPNSSTTSLSPPSFSIPNTMNNSSSSNGSGTVISSISSNITNQLNNLRANNNSFNTSSVNILGKINQNNSNNILNNSNGFTTTTTTTTTNNNSKSSTSSLSNSSSSSSNSLPKIHSSIESLPHAPQQKSELAISNELIDAISSAVPMEEIRSNPLKSNQTIVNNHTNTFDFNHNHNSCNNNNNSATNENPCCPKCDTPATRRHDKRRWWCKKCTKSFTPFRREKRQYGMNGELLGNTAPQCGRCFQQATRKHDKRRWWCKFCKKPFTPTQFNLYDSSHLIYVMGSHKFNENDDQNNTSNNNSPVSMDLSPNSTLSSPTGTTTTTTTTTTTTASMKMNSLLSVNNSHTLKKLKTNNGSPTNYDHSSYEDDEDDEDDDHHSDDEDDDGDHIHELQSEISALNHVNNISKLTTNNNNSSSIKNYDSATLIIDEPETIIPNTSKNPTTTTNGNSSKPNSPNCPIRSLSLTPPPSTTSSSTSTPTASPVTNYFSNKNNNNSNNNNNNNNNNNNNNNNNNNNQPLTNCYDIINTDNVKGILNKKNNNISLSTISSPQSNNNGLNPTSPSTPATTNTTNNNGVIYNILNKTTNLFEPQNIEKKPQQQTQQELQQQKSKSASQILLSSPTSVTVATTTTTSSSITTPTSKQPLPLVLPSPLITSTSPIITSTNTVSLVLPSTPPLINTTPTNLCLSGLTQLGNIGGSVNNNNNIDNDQCGLDQKSKKVRSQTPSEIILENSIETLIQLNGVDKTPSVILSWFRKSDHYKIFDKMVKASQMMDQENKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2P3</accession>
    <name>A0A8J4V2P3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_000070</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ZNF277 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000002">
        <property type="protein sequence ID" value="KAF2078570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2P3"/>
    <dbReference type="OrthoDB" id="278606at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041661">
        <property type="entry name" value="ZN622/Rei1/Reh1_Znf-C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040048">
        <property type="entry name" value="ZNF277"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13267">
        <property type="entry name" value="ZINC FINGER PROTEIN 277"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13267:SF3">
        <property type="entry name" value="ZINC FINGER PROTEIN 277"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12756">
        <property type="entry name" value="zf-C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="220"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="378"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2078570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="473" mass="55803" checksum="41534B4779BCABD4" modified="2022-05-25" version="1">MEQEDFSHWPTISPLSEPLSFTFKKTTITPTTTTTTSTGTCTTDSESESENTPIVKFNCLLCNHFETESRFILEHLFHQHQFVIADVNHVANLSSYLDHWKKQMNGKDLKEYTTVIQTQPTQSQPVTDYYLLSDILPEDQDLRRKLQIERLNFVLNQQHLERINPDFSSVCPMCPVVFKGDRSAVSAHLLDQHNFNIGLSDNLVNINELLDIIREKTIENQCLFCEKTFKSPSVLKLHMKKKKHTRLNPLNTVYDRFYLLNYLEPGKSWEDLKKEDEEDNDNQLNNVELMQQAQNELNGQHHITHWDDWTENSDEDAYMDESAVCLFCTEQFENSDIAFDHMNVIHSFNFDQIRKEWKLDYYDSMKVLNFIRRQIYQLVCCYCGHQFQDANSLYAHLAADTQHCGVKKNNPKWADPQYLFPTYENDSILRNFEGFDEEENNQYLNEEKEYQSQLMDEMIQTRAELMQRITNQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2S4</accession>
    <name>A0A8J4V2S4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein-coupled receptor family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_000061</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000001">
        <property type="protein sequence ID" value="KAF2078690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2S4"/>
    <dbReference type="OrthoDB" id="18453at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022343">
        <property type="entry name" value="GCR1-cAMP_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112:SF9">
        <property type="entry name" value="CYCLIC AMP RECEPTOR-LIKE PROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 157-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02001">
        <property type="entry name" value="GCR1CAMPR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="83"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="236"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="265"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="10">
        <location>
            <begin position="4"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="18"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2078690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="317" mass="36537" checksum="4E1D9DA9642964D4" modified="2022-05-25" version="1">MKDIILIYLICAPLSIIASAFIIISWGVFAKLKHSGSNFIFFQSISDFIFTLKYVATIIIYYTSDINQFDDGEVSNSALCFSIGVWGQFFGQATVMWSFMMTVKVFLQMMRQNQKQYQQQLDKHKPQQVNNTKGTAAKEISTIKYYHLFVWGFCAVNAAIIGAFKQYGPSSNGCWIIGGTNPFRLFELVPLYITISTSIVIMIIILARMKRNRPTSLLPTESVRYKQQEAEFRNQLLKFIFVFVLFWTPPTILRTLEFFEIEAKPFIILDAISVSLQACANSLIWGTSKNFVKLLKRKVRRSKTQNAYLEREYLINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3T2</accession>
    <name>A0A8J4V3T2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008554</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000538">
        <property type="protein sequence ID" value="KAF2070124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3T2"/>
    <dbReference type="OrthoDB" id="24334at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="509" mass="59246" checksum="1175704CD04B5982" modified="2022-05-25" version="1" fragment="single">HHTTPILFGISITDKIKDPIILANDLPQSIKYYKHTNCDKINKDFIPKSVTSVYLDTTSIDIGAIPNSVKIMVLNKPREYEYVDSDVDDGSDGSDGSDDSDDSDYEYYQDDKEELKEEFDIKCLPQSLTQLNFNDEYGIAYINLDEMPPNLLYLNLNECKCVINGNGNGSIKYLLFGIESKELSENFFNIPESVTQLKFGSFYSLEKFNLPSNLTELSYEYWGNAYTLINLPLLPRNLKTLNLNCFLTCESKHNFPIGLINYTNKNQQTNQEKVLIIPDSVRKLQVYNQPTFSELPVIPSTVTDLEIAEYWNLLTMQIKQLTNIIKLNICFDQYDDDELPDELLIGLIPPTVTDLQLNYDCLKPIEKGVIPNSVTKLHLFSRKTVPIYIPNGVTTLTFSYDNQIYPEIEIPSSVRTLGVYRVNFEEFPFHLFPNVTTFWFSESDYYLEFEQKLEMNFPNYNTNILPSNTRLVKFDNRNRYRINYHFIEQKVYKEHYHLANNLLDFKHYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V414</accession>
    <name>A0A8J4V414_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_001575</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000038">
        <property type="protein sequence ID" value="KAF2077128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V414"/>
    <dbReference type="OrthoDB" id="10263345at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990745">
        <property type="term" value="C:EARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019514">
        <property type="entry name" value="Syndetin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040047">
        <property type="entry name" value="VPS50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019515">
        <property type="entry name" value="VPS54_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13258:SF3">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13258">
        <property type="entry name" value="SYNDETIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10474">
        <property type="entry name" value="Syndetin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10475">
        <property type="entry name" value="Vps54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 54 N-terminal" evidence="6">
        <location>
            <begin position="87"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Syndetin C-terminal" evidence="5">
        <location>
            <begin position="844"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="545"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="779"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="546"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="606"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="619"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2077128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1099" mass="126679" checksum="D673036904431CF9" modified="2022-05-25" version="1">MTQSSTNTTLPSLSSLTSLPSLPFKFDVSRLKAKIDEVEAVTKKTFTAAADALGVGVTTTKKPKKTQYYMELIDSTKPSLTEEEFLKITPEGFFQENFDTVQFILDMLPPTNSSSEFSVFLDKNVNQFSRSMDYVNSKLYNRVRKNYTEFVQGMSQIHEIGVELQRSTVMCSNGRRTLAQTKRNLTTTAFVIMSKYSKRNLLQHMYVDLSQIKDIVTLERLLKQTLSDGDYPATIKLYLECRNTITQHMHYRCVPELDSNLKQIYNVVQERIDKDFFNSCREFNSTTYQRVFQAYKLLGRADRILEKLQDYFVDPIEPETRNIVYAHVLLSEENAINPEKFKGLSFKDLCGELKDDHFVNCLLAVFEYLCDVMTSLYLMNQFHLDNDIQEESKIFADINSALTRFKNTIWDTMQRQVSQLLEPRKLSNFKIDDFLLVLNSVTKISEIGEEFSGTPSHNLRKSIDNQSKSYFETLHRKRIEDLRTMLENEPWNNIPVGPDFDVRAELRIQNKGKAKIERLHGDQLFYSIKDKGNPFSELISYKKNKLPSNQSSPNLSSINNSSTSNSNSNSGKSKSKIKSDTEDSDEENEELKQEFIHEDEDDMEAHKKKLKAKQQRIQKQQEEEQKQQEDNMILVSSTTISFVRYIGKYLEIMEMLPHISVDIFNSICQMVEYYMYTIYSFSGYFDPQGFSLEALTSKMEKTISEKSFEDLSFTKPQVSKFIDKMKERVGLPPSTLSSFSTANTVMSGITNISPISSITNQFSQFNLTIKNIASNINSPSLSGQSSQSSQQQLQQQQQQQQYQQQIHNNNNINSTNNNVENGGVKWIPSKINYTGLQLGDPKQFFNLPVRMVAIESLAFISQALIVCKSVFDAILPANQTDNINDFYKNVIDIIPDLQKHLIKCVISAFFTYQAGPQFFSYTISNLKWDFKTMATTKSSYVEAFSKEFQQFIKKLDECIQKSTQVYVTPVINRPLRNKIVSLSFEYFIQQLVDGYSKIKKCTNEGRATMIQDLMSIQNSLEKISGGIKIPNVSYAENFIKAFYQLTAFSEPDILEWAKEHDEYPIRYVIGLLQLAKINKPQFFQQLEDMDRRRRGTRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V527</accession>
    <name>A0A8J4V527_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BolA family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007212</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BolA/IbaG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000372">
        <property type="protein sequence ID" value="KAF2071459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V527"/>
    <dbReference type="OrthoDB" id="4983at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.90">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045115">
        <property type="entry name" value="BOL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12735:SF27">
        <property type="entry name" value="BOLA-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12735">
        <property type="entry name" value="BOLA-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003113">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="88" mass="10083" checksum="5C5EFB60AE09C774" modified="2022-05-25" version="1">MVITTQILESRLKETFKDEKDKITIEDLSGGCGAKFQIIIGSDQFTGLGLLERHRLVNNLISDFMNDIHAVQLKTWTLSQYDEKIKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6Q7</accession>
    <name>A0A8J4V6Q7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_005578</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RAD52 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000226">
        <property type="protein sequence ID" value="KAF2073109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6Q7"/>
    <dbReference type="OrthoDB" id="206565at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045002">
        <property type="term" value="P:double-strand break repair via single-strand annealing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.80">
        <property type="entry name" value="DNA repair protein Rad52/59/22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041247">
        <property type="entry name" value="Rad52_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007232">
        <property type="entry name" value="Rad52_Rad59_Rad22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042525">
        <property type="entry name" value="Rad52_Rad59_Rad22_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12132">
        <property type="entry name" value="DNA REPAIR AND RECOMBINATION PROTEIN RAD52, RAD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12132:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD52 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04098">
        <property type="entry name" value="Rad52_Rad22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="182"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="248"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="182"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="220"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="255"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="289"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2073109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="331" mass="35768" checksum="18E1D477CD13FDBF" modified="2022-05-25" version="1">MISNNIKKEHGDETMANLFGKQQFTEEETNRIQALLGQRPSALNKRTITGDVQSYYLTHKQISDEANAIFGASNWSFRVVSISNDIIDRREEDAEFFIFISAHGRVSLKDGSFRDNLGTGSARLDKTPFHNTIPKVIASAKKKAVTDCFKRCVSMFGPALGNGVKNIPVADSVNNANKRIATNNNQVNSNSNDYVVVNHTNGNSSNSNGNGNHYNHHDTTANSGTMNKSPNKRLADNSAALEATVPLKVENVSSSPPPTSTPTPPSTSSPPKLLNRSPLQLKPTLKIHSTTSPTTTTATSTVTTNTAVVVPPTITDNEDSFSFGMDGDVYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7E8</accession>
    <name>A0A8J4V7E8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001898</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000049">
        <property type="protein sequence ID" value="KAF2076772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7E8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="279"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="309" mass="35099" checksum="9AA75B082DA81E9D" modified="2022-05-25" version="1">MTPKLPSAILMDIPYKPSDRYNNRIDGLIVQCSLDPMKSNLGKLRSAVYISNTSFLLASLITVYSAIEGKIHLLTLTQYSTAINYIFGFIAVASLMLIFLNWISMIQNSLKINNGNLLTKKTKIIFGAYLVPNFIITVICLGMVFHQDFLPDSPNNDSFEKATKVYDYFLLASTVIIVGVLWIYLIWQFKKMNDNTRKRTRKFFITAFSFSFTCLTIPIFMFILESEFRGVFNGWFSFSIQMIYELELVFIVYPLNHHKIIKFFNPNYTLPPDTYSGGTTASSKSNGSLSPPIKGTSFASSKDTINIQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7W4</accession>
    <name>A0A8J4V7W4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UbiA prenyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_001476</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000035">
        <property type="protein sequence ID" value="KAF2077222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7W4"/>
    <dbReference type="OrthoDB" id="203513at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042371">
        <property type="term" value="P:vitamin K biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13962">
        <property type="entry name" value="PT_UbiA_UBIAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026046">
        <property type="entry name" value="UBIAD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929">
        <property type="entry name" value="1,4-DIHYDROXY-2-NAPHTHOATE OCTAPRENYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929:SF0">
        <property type="entry name" value="UBIA PRENYLTRANSFERASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="285"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2077222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="303" mass="34241" checksum="5B64EE777AC010D8" modified="2022-05-25" version="1">MNIVQKYLIANRVSIFMIFVGLTSMTVAVLYKEGKPIRLANTFLVFVSSFLIMAFSCVINTLCDFYSGVDTKDNPNCDRTLFDYGMTPSLLVKFGFFNVIATYTLVLPIVFSPLSSGLGMFKFLAIVTGLLLVSYFYTAPPFQLKYRALGWVGIASGVFFVPHLLYYANTFTFGFKESDLLLSLSTAGPLFCMFLYNGQTLRDIDSDEKAGILTHCVLLGKTVSYYFWFSFYIITMLYITYISIIKNNIFLNLPWMSGIVVYKIYNLTKHNCDFNSKVKKLVPKVPFLCMMLLSVGVLVGTKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V9C5</accession>
    <name>A0A8J4V9C5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_002730</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000078">
        <property type="protein sequence ID" value="KAF2075974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V9C5"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052462">
        <property type="entry name" value="SLIRP/GR-RBP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48027">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN 87F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="3"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="95" mass="10638" checksum="5A879B78A7F3A305" modified="2022-05-25" version="1">MSSRIFIARLPWSVCKIALRNHFSKFGPIQDGYVVLDRVTRRSKGYGFVTFSNQEAAQNAVAANHELEGRQLIVNIAFDKHEQPAGSNNTTTKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VB24</accession>
    <name>A0A8J4VB24_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Parathion hydrolase-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_000946</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="3">Binds 2 divalent metal cations per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the metallo-dependent hydrolases superfamily. Phosphotriesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000020">
        <property type="protein sequence ID" value="KAF2077759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VB24"/>
    <dbReference type="OrthoDB" id="9998343at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001559">
        <property type="entry name" value="Phosphotriesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10819">
        <property type="entry name" value="PHOSPHOTRIESTERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10819:SF3">
        <property type="entry name" value="PHOSPHOTRIESTERASE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02126">
        <property type="entry name" value="PTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016839">
        <property type="entry name" value="PhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51347">
        <property type="entry name" value="PHOSPHOTRIESTERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR601559-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2077759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="365" mass="41030" checksum="8B21F87F408D99DB" modified="2022-05-25" version="1">MEEKRKGKVQTVLGLIEPDQLGITHMHEHIFINYLDFFLKPTDKEQSCCCVGHEDSDHKHVPLEDQKINIKNMHWVQYNYNKNLHNLQLDEEDVAISELELYKKCGGKTILDVTTEGIGRDPERCKRVSQKLNLNIVVGAGYYLEKTIGKLVSNTPVEDMEEKIVKQVLEGIDNTGIKAGIIGEVGCSYPLSANETKSLIASARAQKRTGASITIHPGRSTKSCSEILNVLKDNGADLTRVVMGHIDRTVHDLNIMLDIAKTGCVLEFDLFGMEISYYPFGGDVMGMASDNQRIDWIKSLIDAGYGKNIVISQDIYTKHRLVTYGGHGYHHILFNIVPRMLKHGITQENIDDMLINTPKRILTIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VBB6</accession>
    <name>A0A8J4VBB6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_000667</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. BUD32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000013">
        <property type="protein sequence ID" value="KAF2078029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VBB6"/>
    <dbReference type="OrthoDB" id="3399at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070525">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000201">
        <property type="entry name" value="TP53-regulating kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000323">
        <property type="entry name" value="TP53-regulating kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022495">
        <property type="entry name" value="Bud32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03724">
        <property type="entry name" value="arch_bud32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209:SF0">
        <property type="entry name" value="EKC_KEOPS COMPLEX SUBUNIT TP53RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209">
        <property type="entry name" value="NON-SPECIFIC SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06293">
        <property type="entry name" value="Kdo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="21"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2078029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="254" mass="28768" checksum="D08F55AEE544EB98" modified="2022-05-25" version="1">MTTLATTNNNEIKSIENITSLPNSVLLSQGAEAKTYKIDLYGIKCIVKERFVKAYRHPTLDQKISGKRILQEVRSLNKCKKNGIDVPALLLVDVPSNRIYMEYIDGITVKQYLYNCFDQSKETSTTTTQTQTEAHQQQIDICAEIGKQIARIHDLNIVHGDLTTSNMLLRNNSKSNIVFIDFGLSYTSTMTEDKAVDMYVLERAFISTHPNSELLFKEILDSYQKNSPSPKVAKAILDKLDQVRLRGRKKLAFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJB1</accession>
    <name>A0A8J4PJB1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010266</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001005">
        <property type="protein sequence ID" value="KAF2068410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJB1"/>
    <dbReference type="OrthoDB" id="659430at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="12973" checksum="58B9246C6474ECF5" modified="2022-05-25" version="1" fragment="single">SKPSITSSSQFPPNLKKLALPCYDGVLDIIPTTINHLEFNRNISKQKYVTFPIELVPPHITTLVLNDSMRIQSYDLIPASITSITLCNSITPYTKIPCTVESVVLPSSFNQPLDTII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJU3</accession>
    <name>A0A8J4PJU3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010432</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001108">
        <property type="protein sequence ID" value="KAF2068242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJU3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="102" mass="11557" checksum="85F1F24258C34813" modified="2022-05-25" version="1">MSSKDDLELVMGLGSLVSRIQFGTSVIQALMNFSITVQPSLSLTTSSLLLKACNHSFFLYSTTWVEDDQDTEEEIDKHPTIDKLITACEYYSERECCVFYQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PK00</accession>
    <name>A0A8J4PK00_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009591</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000752">
        <property type="protein sequence ID" value="KAF2069087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PK00"/>
    <dbReference type="OrthoDB" id="429520at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20916">
        <property type="entry name" value="CYSTEINE AND GLYCINE-RICH PROTEIN 2 BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20916:SF18">
        <property type="entry name" value="IPT_TIG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAP" evidence="2">
        <location>
            <begin position="159"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="319"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="430"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="271"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="332"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="432"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="548" mass="62163" checksum="FCF984301939F0AA" modified="2022-05-25" version="1">MISHQFQQQCAFDKCSICLGDISFTPSKDITSFEEDNKKLMFEYFEKSTIIGRVCQSCINQFLRYKINLNKKSIEFDNTLPPLPLSQIESNNGFFQSTSPSSFDRFSPISPPTVLNNSNNFNNINSNNNNNNTTTQSNICPPTDDSKSKKLIDICVDYLKTLKTIELHDLLKKIKASKKGSKSKLIERLIRHMFAFKKDNPIPPLNTSTMTEKDLQNTLKKYGATFVRGSRSELIPRLLVYHYLLGGYETCAISTPTTSGNRVSKTPSPRNSIHNSFNNLNNNNNNNTNSKTTTTSNHPTSVQIPIVNNYFRLNQTLESPLSDSVSPRYDYPTPESSPTTSFPSSPNFQIPSSHCFSSPMSSPVSFCNINNNNNNNNNNQLSFLPKLSSSYQNNNNFTLTSNTLPSHVLDNNGSNSSPTHLISNIQTLYNTSSPSSPVQSSPLIKSYQQQQQQIEQPQQLQQQPQQQSVNPQYNNDYFKEYNNVNYYPQLYFDCAPQNSYNDYNLYNNNYYYNSNANNFNNNDNNNNNNYFYDNGASYSIPTISTTTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKL8</accession>
    <name>A0A8J4PKL8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000919">
        <property type="protein sequence ID" value="KAF2068583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKL8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="329"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="378"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="470" mass="53153" checksum="CC4354B2476AF3DC" modified="2022-05-25" version="1" fragment="single">MSSSFSKLLSLPELGQVLDQVFIAKVEQVLKRLKSSHNTGACDAEEKKVKKGREKTKGSKGDNGESRYVEFPKGYIPLSVRTLSIEPSNSEIRPKVVYKGEWIPSSVTKLAIPSLPFPLSLVPEHITYLYLFGQEEPIEIDMVPPTIQYLWLTQSNLSHDLPNTIKELALTTKGPCDDIYIKPTFTSPIQLFYKCSEGSSDIQVVSNSKLNIQTIIFFKRPLRLNAITLPSTLTSLTLGPDTFNHSLAALDPLPPHLKYLSLPDTCGPIYQNQHLPITLTHLRLTIRTDKKTNIFSNDWLCRKGYLPPSITHLQLSILIKLDKDQEYKEDDEMATKEQEDVDEMATEEQEVDDDNQIDADHQLKKRKLNDGSHGTTEQEQEEEEEEEVTLPSALKILLPSSIQSICLEPDDDLRRSRITVIDRDDYYSTTPTPPKIREGLKELVLPESFNQFIPNNTLPSTLESLSILNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL42</accession>
    <name>A0A8J4PL42_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Carboxylesterase type B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008807</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000582">
        <property type="protein sequence ID" value="KAF2069867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL42"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxylesterase type B" evidence="1">
        <location>
            <begin position="13"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="119" mass="13487" checksum="79C5A35B770A6882" modified="2022-05-25" version="1" fragment="single">SGSPVTDECEGLTCHGDELSMIFNTYFSALGNNLDDHEILMAQDLSNYWVNFIRNNDPNIGITTETMPHWTRINSVNDSLVVHDSLESKDIISLDERCSMFDDISYRGYTKPTAEICPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL54</accession>
    <name>A0A8J4PL54_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000925">
        <property type="protein sequence ID" value="KAF2068569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL54"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="134"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="210" mass="24300" checksum="7E42702948ABDCDA" modified="2022-05-25" version="1">MGIYVILGEDGLMPEYIGSTSQLTEKGYSSRIKNISHDDRRDLYKSFCKFDLKNNPPRYYAWLIEQIMLSSFVYKHNSKVNYDFNTVDESSDQFFLYVEYLMYNYDNYLKEMHSEMKKLSFDSDPNEQFTESQPETPVEPETPVVPETPPRNKNINNDNDSDADENVSIDKKPESPTKGHNPLTISPKKNGGASKKNTPKSKKILLRKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLY0</accession>
    <name>A0A8J4PLY0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Succinyl-CoA:3-ketoacid-coenzyme A transferase</fullName>
            <ecNumber evidence="7">2.8.3.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_009221</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 3-oxo acid + succinyl-CoA = a 3-oxoacyl-CoA + succinate</text>
            <dbReference type="Rhea" id="RHEA:24564"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:35973"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="2.8.3.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Ketone metabolism; succinyl-CoA degradation; acetoacetyl-CoA from succinyl-CoA: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the 3-oxoacid CoA-transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.3.5" evidence="7"/>
    <dbReference type="EMBL" id="AJWJ01000658">
        <property type="protein sequence ID" value="KAF2069457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLY0"/>
    <dbReference type="OrthoDB" id="1933379at2759"/>
    <dbReference type="UniPathway" id="UPA00929">
        <property type="reaction ID" value="UER00894"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008260">
        <property type="term" value="F:succinyl-CoA:3-oxo-acid CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046952">
        <property type="term" value="P:ketone body catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1080.10:FF:000001">
        <property type="entry name" value="Succinyl-coa:3-ketoacid-coenzyme a transferase subunit b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1080.10:FF:000002">
        <property type="entry name" value="Succinyl-CoA:3-ketoacid-coenzyme A transferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.10">
        <property type="entry name" value="Glutaconate Coenzyme A-transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012792">
        <property type="entry name" value="3-oxoacid_CoA-transf_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012791">
        <property type="entry name" value="3-oxoacid_CoA-transf_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014388">
        <property type="entry name" value="3-oxoacid_CoA-transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004165">
        <property type="entry name" value="CoA_trans_fam_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004164">
        <property type="entry name" value="CoA_transf_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02429">
        <property type="entry name" value="pcaI_scoA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02428">
        <property type="entry name" value="pcaJ_scoB_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13707">
        <property type="entry name" value="KETOACID-COENZYME A TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13707:SF23">
        <property type="entry name" value="SUCCINYL-COA:3-KETOACID-COENZYME A TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01144">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000858">
        <property type="entry name" value="SCOT-t"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00882">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01274">
        <property type="entry name" value="COA_TRANSF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="active site" description="5-glutamyl coenzyme A thioester intermediate" evidence="8">
        <location>
            <position position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000858-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2069457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="508" mass="54628" checksum="17000F70A027908E" modified="2022-05-25" version="1">MLKRNIISNIGLVARYYTTKSVGSNKIYNSADEAVADMPQGAKLLVGGFGLCGIPENLIEAVRKKGVDKLTIVSNNAGVDDFGLGVLLKNNQVKRMISSYVGENATFESQYLKGQLEVELTPQGNLAERIRAGGAGIPAFYTSTGVGTVLVEEGGFPIKYGKDGSVEIKSEPRETKMFNGKKYCLEESITGDYSLVKAWKADTRGNLIFRNTARNFNPPMATAGKITIAEVEEIVEPGQIKPDEIHVPGIYVQRLIKGPSYEKRIERLTVDKGQAAKDTNAKPKNDAALKRERIVRRAALEFKDGMYCNLGIGMPTLASNYIPKGIRIELQSENGLLGMGPFPKLGEHDADLINAGKETVTTIPGSSIFSSSDSFAMIRGGHVDLTILGGMQVSKNGDLANWVIPGQMVKGPGGAMDLTSSGSRVVVTMEHTSKDGKPKIMDSCSLPLTGKGCVNRIITELAVFDVEKGGLVLIEIADGTTVDQLKSLTGCSFTVSKDLKPMQQVSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM65</accession>
    <name>A0A8J4PM65_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_009058</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNase II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000627">
        <property type="protein sequence ID" value="KAF2069624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM65"/>
    <dbReference type="OrthoDB" id="24386at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004531">
        <property type="term" value="F:deoxyribonuclease II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004947">
        <property type="entry name" value="DNase_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10858">
        <property type="entry name" value="DEOXYRIBONUCLEASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10858:SF23">
        <property type="entry name" value="DEOXYRIBONUCLEASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03265">
        <property type="entry name" value="DNase_II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035249470" evidence="4">
        <location>
            <begin position="22"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="500"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="732"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="510"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="767"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="790"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="820"/>
            <end position="845"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2069624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="845" mass="96237" checksum="C8D23DA3433A5B3E" modified="2022-05-25" version="1">MNRCITIYLLVFCIFISLCYADKGTFENYCQVPQKTATGVSWKQVKWWMVLKDAGPISNKLKYALFYNDGSKTVIETSNSDHHGVGFKDFIETVSLKNCGADGKYYFQSWFHEGSHIKEKTDNFDKPKFSQGFSKAHAKYLISHDAQFNGFIMDHSLPIPHDDGVFGKSTSTNPSQHAFCFNFNGADFDMIVKLFQVSMPMIVKTNGDDKVCKKNSSPLWFNYNAKDNLPKPFPPERHLIYKFNQNCKIAMEVRAKTIVKQKGDVPKRLPFLEVMESDYPEDCYKTLTFLNGIKAYGFDHNCFTSSKSTFPITKTTTPAGNTVEESEDYYSWRNNFYNEKCTENVKQKDYNRKDTHLCSPVEFGIDSWLAVSQLEKKLFLVSTQNQNHKLPSFIKTSDKMEWGVVDINSFFDTTNRHEKVGVEYGQGTRVCIGDSNRVQGQLHHLGNIFCLDDKDLANALRTMIYTEKKTVKKRFNTINLYSTNPNFKFLDSDFTIMDQKTNPDPSPPISTATGSDASSSGTKKSSSSDSRYPSFFYPNAEVDPIDVQILSDEAKYPDCSLYKLNPTEVKNYLTESKISKELNVRTDYCKPLFETVVNEHFQKVTIKDIYSKLRGFKQNDEDPTILEEDEKTTASLKNKYKSKEEGGGERGGYNRYNPRFLRKYLNCWAETTYPDSFENAYDFKISEVEDVFLERIEQVMMNPIPDYYTESCYYTDIIDLTFGKAAITTKECDAQPPRCPPPVPPKVNNKKRGRTETTGENEGPQSKKPKGPSKNRRAHSPATTSQTHPSAEKTEFFDPSKPKVSFEEATTTKCREYRPLDSLTDPSTSSSAPSSSAASSSMVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PME1</accession>
    <name>A0A8J4PME1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RWD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008969</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000609">
        <property type="protein sequence ID" value="KAF2069713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PME1"/>
    <dbReference type="OrthoDB" id="277175at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009893">
        <property type="term" value="P:positive regulation of metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051246">
        <property type="term" value="P:regulation of protein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23823">
        <property type="entry name" value="RWD_GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000050">
        <property type="entry name" value="eIF-2-alpha kinase GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040213">
        <property type="entry name" value="GIR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12292">
        <property type="entry name" value="RWD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RWD" evidence="2">
        <location>
            <begin position="7"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="234" mass="27004" checksum="C0417725CF777F27" modified="2022-05-25" version="1">MTEERDMEIEALGAIYMDSFNLIDQESIQITLLPNPGQDEPNYVGVILDIKFTPDYPNEVPIIKLTPQLDLTNEQLEEIKELVTTQANENIGTSMIFILTSVIKEWLDNNNHEPKEESEEESEEEEEEEEEEEPVFEGTQCTMETFNAWRKKFNEEFRPVKKETKSTKLTGRQLFEYDATLSISDSKLMEEGEETSNISAASIKPKIEEVAQQVDWSLFADDDVPEDFDDEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMI3</accession>
    <name>A0A8J4PMI3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ribulose-phosphate 3-epimerase</fullName>
            <ecNumber evidence="7">5.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_009136</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-ribulose 5-phosphate = D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13677"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="EC" id="5.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ribulose-phosphate 3-epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.1" evidence="7"/>
    <dbReference type="EMBL" id="AJWJ01000643">
        <property type="protein sequence ID" value="KAF2069545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMI3"/>
    <dbReference type="OrthoDB" id="1927044at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004750">
        <property type="term" value="F:D-ribulose-phosphate 3-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006091">
        <property type="term" value="P:generation of precursor metabolites and energy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006163">
        <property type="term" value="P:purine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000191">
        <property type="entry name" value="ribulose-phosphate 3-epimerase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000056">
        <property type="entry name" value="Ribul_P_3_epim-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11749">
        <property type="entry name" value="RIBULOSE-5-PHOSPHATE-3-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00834">
        <property type="entry name" value="Ribul_P_3_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01086">
        <property type="entry name" value="RIBUL_P_3_EPIMER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00057323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2069545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="158" mass="17465" checksum="2F1D64138D3F16C7" modified="2022-05-25" version="1">MVSNPELMIDDFKNAGANSITFHIEVTEERGITQSVIDKIKAYGMQVAISVKPKTPIESIFPYVKDLDFVLIMTVEPGFGGQSFMSEMMTKVSTLRRKYPNLNIQVDGGLDIKTIEPAAAAGANVVVAGSALFRPDQNPKDTIQFFKDTINKHSPNWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMN1</accession>
    <name>A0A8J4PMN1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein-serine/threonine phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_008911</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000597">
        <property type="protein sequence ID" value="KAF2069772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMN1"/>
    <dbReference type="OrthoDB" id="285418at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000004">
        <property type="entry name" value="Protein phosphatase Slingshot homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF414">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="9">
        <location>
            <begin position="34"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="7">
        <location>
            <begin position="177"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="8">
        <location>
            <begin position="240"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="327"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="397"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="412"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2069772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="438" mass="50671" checksum="EA6FF6DEC029F400" modified="2022-05-25" version="1">MVFIENNLNFTVKEGDIGIYHINSMDFYNILQDISSNKLIIDIRDQDSFTQGHVRCAVNIHPPTHYSANELQKFNMAKYIGENVTAKHWNLIFNRIILYSDKPFFYNTDDLSHASMDTIPNVQPLSLLDWDESFLNHMVSRKKKINVIYVYQGGYDSFYKEYPFMCGKSKISFADLYPSEIIKNFLYLGGKENAEVLVQLKNLKITHIVNMAIELEDYYPHQFKYYRANLEDRYYANIHQHFLPIIDFIEDAKKVNGRVLVHCAMGISRSASVVIAYLMKTMQLTYVESLKFTKAKRSMINPNQGFVKQLKAFDLELLNLKKQKIIDDNNSNSKNNTQESLEKQNEKDQQEQKVDKVKDNTNNSNQDEKLLQDINNTNNLILDDSTSKEEKLLKDINNNNNINKDEKDANDANDANDANDANDANDANDANKHPISLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNQ3</accession>
    <name>A0A8J4PNQ3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4209 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008867</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000590">
        <property type="protein sequence ID" value="KAF2069816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNQ3"/>
    <dbReference type="OrthoDB" id="49386at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025209">
        <property type="entry name" value="DUF4209"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039635">
        <property type="entry name" value="ERMARD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31701">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MEMBRANE-ASSOCIATED RNA DEGRADATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31701:SF2">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MEMBRANE-ASSOCIATED RNA DEGRADATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13910">
        <property type="entry name" value="DUF4209"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4209" evidence="1">
        <location>
            <begin position="189"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="787" mass="92335" checksum="ADAFA3427BE296D9" modified="2022-05-25" version="1">MHIETLPSYLSTNVYDLLISSDKKIFNVDDKCAELLLLSDDNLTFKSSFHNIFHLKEKDESLDFYITSINDDQIEQQQSFSKLLEFFNVNSKYIRYISENNLKVILNYSDTQLNQYIDLWFSNEYLKSKSDNDIPLIPHYPLFIQCLESIKSHLFRLKDHSSDNNNNHNNNNNDILDLNYLYSIIFSIIERMIGDIIYCEMILDGKTPIITRMLRDLLDDKYLHNVLDIDIILLLKLIIGPPIGLNFRNIIWHGFISPYQLKIDMLLLSVHLIFTISKRYQLYLQSNSKKTITPRSLLNYEKKFNSLGQLELKFNNQSITGDILKLSSPTKIDDLINNIIDSSSFIIKGRNQQWKNAFKRYFNSLQQIDNNSNDRFISLVTLLPQLEHSLRVKFVESNQLSNQFLNADYTSYYTTLDLFLAPTLWLNNSDDTNVKHLQIPTLNKSYSKYENPKYSKFKSEESSPSKSPQNQELEIIEFENKLVKDIGYGCMGILLDLLMYMDGPRLRDRIAHGEIDHKSIPLVFSDLLMILSLDLCIKYSTKNSQQNETIQSISSFTDQYTPMFHTKSIIISKYQQMNQSINNFTQFYNQLNCSHVKSNENENEENQEDLISDYIGDKKYNPIDIKSDILDKIVEYYNTLQSKDININNNNINLFPEKLELDIFKVLGKSIQIVLETTNVFLEMGKNLYDKIFNLPTNVGKNKTQKNSLYKWIGVCESMILLLNITNIQIQYYIYDSNTHCLESIKLIEKIQFYFEKLLNKTKLFEWDNLIKLVYQYYSLVLLKNTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP52</accession>
    <name>A0A8J4PP52_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007101</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000360">
        <property type="protein sequence ID" value="KAF2071592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP52"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="151" mass="18144" checksum="62D5451835ED8E95" modified="2022-05-25" version="1">MSSSNNNNQQQDLLEALTIISKSKTSELKKEHCRKILKKFQFEAQFIIDANNRNCVDDYLESITEEYKYSLFEQQREQDQKQNLKKKQSFIRVYKDSKINDDEDHDVDNDNVIYPQQHINGEINLPFKIRLDFFIEKLNSFFKILHIKYQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP80</accession>
    <name>A0A8J4PP80_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase C1A papain C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008467</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000525">
        <property type="protein sequence ID" value="KAF2070206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP80"/>
    <dbReference type="OrthoDB" id="10259130at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="170" mass="19243" checksum="301E15B938564496" modified="2022-05-25" version="1" fragment="single">FSEQELLDCSSRYGNSGCSGGWMHNCFRYIQENRGISQEKDYPYVGRVEHCLAFGKIKHAPISRYDMIPQHNENNLADVVAFHGPVSVAYDASTREFMYYTKGIYFSESCDQNRVTHAVVAVGYGTENGVDYWILKNSWGADWGEKGFFRMKRNTGDKCGIATASSYPTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP92</accession>
    <name>A0A8J4PP92_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Lysosomal integral membrane protein II</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_008444</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD36 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000521">
        <property type="protein sequence ID" value="KAF2070236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP92"/>
    <dbReference type="OrthoDB" id="18585at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045335">
        <property type="term" value="C:phagocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005044">
        <property type="term" value="F:scavenger receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006911">
        <property type="term" value="P:phagocytosis, engulfment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002159">
        <property type="entry name" value="CD36_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923:SF102">
        <property type="entry name" value="LYSOSOME MEMBRANE PROTEIN 2-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923">
        <property type="entry name" value="SCAVENGER RECEPTOR CLASS B TYPE-1 SR-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01130">
        <property type="entry name" value="CD36"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01609">
        <property type="entry name" value="CD36FAMILY"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="726"/>
            <end position="749"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2070236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="758" mass="83265" checksum="0D6F0A09ECC274AD" modified="2022-05-25" version="1">MKNLGRLISLAIGLILVAVGIIIWVKVDDAIHKEIRNADIVLPENDKDEVVNPWTRFTGNENDPNNERIYVFYAYNLTNPVETANNEFPKYTELGPYYYKYVYERMNHTLLEDDTIVQFQFWKRYFPLTPDQVPQYPNARNPLTDEIYHFNLAYGAILSQTQGELGLALTLVSATATKLISSVNTLEFQDQVLAASSCSVLGQTFAGVKAAMGSDAAACAAWAGSATVSPSVPHFAVGSFSGVPSTISEAKCLSLFNPAKPYSFTNSSATAEGFGLWAKAATYPNPFAGLIKQGVMAAHGLTSEEFDIVFDWMNKFKNADVLAAVLSTTDICPSGTCAKADLGYLQWANKNGVLGGKSLADLDKTLPGKVEFGLVEGMSLNLASTKILFNTASPINLVTAQGLGAFLSLLKAQDYPKIIAATKMSQTEIPKLYEYYTYVQNLLWGAIKAKNGGPIVKHTLNSLIFDSVDPILATLYPNDESKWKSNPLSNITTREEAAATLPFDQSYTGKDDTDKAQSPKTFEGHPYVKYAEQIPVSGYHPEQLPPYLLGEHSNPAISVFSEEYARALTMERHIVGSLEGLPYYRYTVTPDNWAANPLYFTEIPYLLNLTSELGIPVFISRPRMKGLNVGYFSKSGLADMAYDTADLDVFADYEPRSGKAIRGRYSLQVNAYIPGDDEATSPLYGTLTKLRGDVVHPMIWGVNEISATQKQIDVIGQAYSIDAFRYALTTILMVVGGFLFLVAGGLFVLDIFEKKGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPZ7</accession>
    <name>A0A8J4PPZ7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Trimethylguanosine synthase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Cap-specific guanine-N(2) methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_007227</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78479"/>
            <dbReference type="Rhea" id="RHEA-COMP:19087"/>
            <dbReference type="Rhea" id="RHEA-COMP:19089"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:167623"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:78480"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78507"/>
            <dbReference type="Rhea" id="RHEA-COMP:19088"/>
            <dbReference type="Rhea" id="RHEA-COMP:19090"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:167623"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:78508"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78471"/>
            <dbReference type="Rhea" id="RHEA-COMP:19085"/>
            <dbReference type="Rhea" id="RHEA-COMP:19087"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:78472"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78475"/>
            <dbReference type="Rhea" id="RHEA-COMP:19086"/>
            <dbReference type="Rhea" id="RHEA-COMP:19088"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:78476"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. Trimethylguanosine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000373">
        <property type="protein sequence ID" value="KAF2071453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPZ7"/>
    <dbReference type="OrthoDB" id="194443at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071164">
        <property type="term" value="F:RNA cap trimethylguanosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019012">
        <property type="entry name" value="RNA_cap_Gua-N2-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741">
        <property type="entry name" value="S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741:SF32">
        <property type="entry name" value="TRIMETHYLGUANOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09445">
        <property type="entry name" value="Methyltransf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="22"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="41"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="70"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2071453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="367" mass="42493" checksum="7E497E1A11257216" modified="2022-05-25" version="1">MEEYFKKRIDILEKKTNDKSFDTLPKAARKRQRKKLRSLVIQRNQWLKKTSAAAHSNDSTNNFSNESQKNISTNISSSTTGKRQLKGKVVKSQTTATRKKVDESTDESESDEDEEEQEEEEEKEKEENGFIGFLPQTKENQKYYYQRYRLFSKFDEGIILDEESWYSVTPEKIANHIAERLRSNIVFDAFGGAGGNTIALANTCNIVIHNDIDPIKTHMAKHNSLVYSHTQENTNIEFINCDFMAFSESYFGTRSTLPPIPLDHHCDCIFLSPPWGGPEYLQKEHFYIGTMPIDGFDIFRQALKISPNVAYFLPRNTHIDDIRKLSKIYKDLGYYPYDCEVEENYLNESIKSITIYFGNLINRAESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ72</accession>
    <name>A0A8J4PQ72_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000358">
        <property type="protein sequence ID" value="KAF2071603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ72"/>
    <dbReference type="OrthoDB" id="60283at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="686"/>
            <end position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="720" mass="84407" checksum="D2F9E937DCB01082" modified="2022-05-25" version="1">MDSTKLLYTETYNQKTTINQLIHTFLKRGGNPEHITKGTATPLGHDEIFKVDWIIENQLFSLLKEKISKNKYLLFDKNSIESIFKIKDLDLFIQVFNKFEDYYSPRSEILQLAVKHDNFEISKYMFEQKGYLFNLDLSLEYAWRNENYEMVRYLLDSHYDRINFRNGASCINIEHSIQSGDLEMINYILGPKFCDGVKFSNASLIYFHKLAFYTGKYEIFKSIHEKFPLNQADLLALQDWFQVCSCKKGSHELLVYLKEKSFIDEFTYQSFRNIMGIEAFKHQDMDTFNKLNNGECEIQIQTLPISSVKDSFTSFQKVVELVNLKLQITKDCIKNAASKSWDLASLLGNNCVELVNGLVEYYQMDIEPISTNSNITNCSTPPTKNFFNQLLVKDENVDLLNEYLINFSHLLVASDYEEALKLSAHYGTVEIFKLLFTKIYNTTNNNNLMDQFNTIVPPMACSNAKKDLFYNINEIKKYRFSHLYNIAATNSNNGILRYLIEQSIPHDGTVLWKASSLETIDLLRSAGHSLESCLLEANMEFEFELPSHPLFLHLLQCHQLHRYNIDHLILASIKQQKIKQFKYLIDHANIHCHSHVVAIIDNLEMLQYYLEKYPPYIYNSIVDNGYKECFIRSMKLGRLDILKFLIQSFDPTIILLTSIKIKEFKLQLNQCDEYVRVFLQHQVDKNTNPSTMTKKKRKRDRQPHEKRDKEYILFSFLTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQE9</accession>
    <name>A0A8J4PQE9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosome biogenesis protein BOP1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_006536</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for maturation of ribosomal RNAs and formation of the large ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat BOP1/ERB1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000307">
        <property type="protein sequence ID" value="KAF2072138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQE9"/>
    <dbReference type="OrthoDB" id="5571054at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070545">
        <property type="term" value="C:PeBoW complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000061">
        <property type="entry name" value="Ribosome biogenesis protein BOP1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03027">
        <property type="entry name" value="BOP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028598">
        <property type="entry name" value="BOP1/Erb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012953">
        <property type="entry name" value="BOP1_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17605:SF0">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BOP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17605">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BOP1 BLOCK OF PROLIFERATION 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08145">
        <property type="entry name" value="BOP1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01035">
        <property type="entry name" value="BOP1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BOP1 N-terminal" evidence="9">
        <location>
            <begin position="228"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="498"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="9"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="35"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="87"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="102"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="146"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="160"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="172"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2072138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="836" mass="95665" checksum="9F4D7405D0C6E783" modified="2022-05-25" version="1">MAIDKKPTTKTTAAKKPAVAAAAAPAKKDEKVVAKKTPVATPAAAVKASTPVKVAPTKVAPVKPTTTAVVQQKQPQKQQPTKSTPQPKKEPVIENKRKTLEESEDEEEEEEDELDFGEIREDISEDEGDDEEDLDQEDQDESEQESESKVDLDSLVEYRSDDEEEEEEESIKSNTTSSKSGIDSSEDEDTTVIGKKWTSDPNQFYDDEDSSEDESLVNRVGNIPLEWYEDYDHIGYDVDGNKIMKTESNKDSIDKFLDQADPNFWRTVYDKVNDKNVLLSPEDMALLKNIQNRQFPPDYDPYEFWYDPGPNPDSIHPMHNAPRPKRSFFPHGTDEEREIRRLTKLIRAGWITFDKKDKKTHTYDIWGEGAKTDEVELNQDKHKAITRIPAPKQRLPGNVESFNPPEEYLLTDNEMKAWHLMDPRERPHNFIPQKYNFLRHIPTYNKLIKERFERCLDLYLCPRTKKVKQFTKDANQFLPKLPKPQDLRPFPSHEEIQFNGHTDRVRTISVSPNGQWLASGSNDNTVRIWEVSSTRCVYTLQVDNEVQCVAWNPNPLFNILAIAHNNKVVIITPPLYGPANLQTADTERILTKPDAHSSSKNVDWYAPSDSASVEKGVRLELHLAFKVVNITWHFKGDYFSTTSPEEGSKSVKIHHLSKRASQSPFRRSKTPNQVSKFHPTKPIFFVADQNIIRVYDLMKQELIKKLITGCRYISSIDIHPGGDNVIMSGYDKKVCWFDLDLSVKPYKVLNYHKLAVRKVIYHPTLPLFASCSDDLSIHIFHGMVYDDLLQNALIVPLKILKTHQSVNDLGVLDIAFHPKQPWIFSSGADSTIRLYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQW6</accession>
    <name>A0A8J4PQW6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000277">
        <property type="protein sequence ID" value="KAF2072460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQW6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="228" mass="26282" checksum="8F597C1C014F1AAF" modified="2022-05-25" version="1">MSSTGTGIRYELELGKGKGCCGVEVESYPGIFDGVLSKEEYDQVVNKYYKARRVIVPYIAPFVYAIVLTAVIVLNLKFYNKGLLIFLAITVSMMFLIVMGINISRKRTRMNETTFRMNMQYESRGIKFIYRAELNYDIITIEYNRPNMVEEVQQQQQQAQQLTNTLDTQNGYSHTMIEIDGCSQPMTYDHYPTPSAPLQLYTDTYQQQCQVDPHCSDSSYIVNLDKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQW8</accession>
    <name>A0A8J4PQW8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007481</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000402">
        <property type="protein sequence ID" value="KAF2071195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQW8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="121" mass="14711" checksum="A9DB5C440EC94D20" modified="2022-05-25" version="1">MKIILNQKCKDTSISIYRIFTRLSNKFPNHQRNRIKQNTKYAFQQNRFLLDLGSFNQSYIDDLNKKSIRLYKCLSYISDRPKIFNQLFPPSSQHYDIKQFDDLLKQQDHQFNDKENENRNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRR7</accession>
    <name>A0A8J4PRR7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Myosin-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_006533</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000306">
        <property type="protein sequence ID" value="KAF2072157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRR7"/>
    <dbReference type="OrthoDB" id="21151at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017022">
        <property type="term" value="F:myosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026859">
        <property type="entry name" value="Myosin-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12632">
        <property type="entry name" value="Vezatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin-binding" evidence="7">
        <location>
            <begin position="202"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="44"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="631"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="668"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2072157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="735" mass="82809" checksum="921493CEA67F49AC" modified="2022-05-25" version="1">MSTADVHELILENSPFDQYLKSLDIDQDQFQGDILSLDDFNDAVEESENDTKGDDHGTEKSGHDGVSRRDGDGDGGGTLNRVEQRLMEYYQSLTTSIVDSNILHPFESPFINQPNNSLHQTDQVLPPLYPLSLYNIQIDGNSGNDEGDGQQRQDDRMLVIEVLMGGVKRMKWRVWAYALGGMAVLVALLGWMYVCMGTAGIVMAMIAVVSVRWKEIKQQNVAIKRVLDDTLTITKTHIKSLAKAIHLVREVELISRGYRLSTPLTPIERLEVSSPLNGNKSEKSLILRAGISRSLRSSIALFRDCIQSLTPFLRPQERVYVRSQLSDLFNDTSNNDTNNDAVSIEEMDTQLPILTLKAMLQEYEIMVSNYFTIVVFVLSKVHLSSDTSMLLAGHHRSMSTLHNSLNQVYSRLLIENNSLKSLVSKEQWDNKIPKRVLLSKPKKSTIESFLFSTNAIAESLNSMSPLFSILHSKIAPLEKQEQEDNNNGDQELQSTIDLVDILAVDEVVNDFVKIKQELSQCMRNWENANRALIKIVRSIPIEAKDGSKQQWDQREAEERVKRLLDKLRGYDSANLDYIAEPTDPDQHAMTEDDNYTVLHNTDETIYEAESGFLDETIEKPLSIDTPVNHHPLSTANDGTVPATGLPPPPPPPPLLGMVRKAKPSKRTVFDPLFDPQTNQMKKKNSPTPNPTEASTDQPPVYNYRNLIPPEKKVINELLMVLDTHKNNNNNNNNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSC4</accession>
    <name>A0A8J4PSC4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_007121</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000362">
        <property type="protein sequence ID" value="KAF2071569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSC4"/>
    <dbReference type="OrthoDB" id="2162994at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052138">
        <property type="entry name" value="GATA_ZnFinger_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47255">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR 22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47255:SF4">
        <property type="entry name" value="GATA ZINC FINGER DOMAIN-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="6">
        <location>
            <begin position="609"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="99"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="223"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="454"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="533"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="701"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="99"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="188"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="223"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="248"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="265"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="296"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="313"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="338"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="469"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="486"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="501"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="537"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="701"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="765"/>
            <end position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2071569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="781" mass="85019" checksum="FCA3F2F1858651F7" modified="2022-05-25" version="1">MDNLNNNIIDTRINKPFFDSSSSSISSLSNSHSDSDLYGSFKSHFIQQNSSNSSSSSDLSHSMSSLTNYHQQHNVADNQISNVDSSKIQRWSQPTMMQQPVQYDNPTGNSENSTCNNSPVNQSTNYLPNPILSTNTTAPNSTNLVSTLPSNSPSVLSAPQKKRGREDDNTNATSYIDPFFLRGDQVCSDPSQNINDQDGLNSNSNDPKELNIKIKKDAQGSNIVSKTSNTSSTTVTSTSSSLKLPSIHHLEFEVERSPKKDDGASTNNQSQSGSPTNSSSNSNNANTQKKRKTKPSKYTITSLNTSGPTPLASTSPTSSSLNSNSTNNNNNNNVTNNFMGMNSGFSMNPSMQQQQPIQQTTFLNSSNGNLGTSGDYILSNNYQSPTSSSTATSSVASPLINQYPMHNQLYNSMPNDGYFQQQSSVLLSPFPKSTTTSQYSNLQQPQQQQIQIQHQQQQIQHNDPNDYMTNYYNNNNTTTTTTNNNGLTQPSYNDYQRSYDSSSSTTSTSSTPAPTSTSSSSVASSLALLSSLGDSKPSYSSYNSATPTPAVSSKSHSIKFENTTNPYNHHEPSQITQPHLYQNNFTYYDQSFPHAPLKKTHRRRPANIDKSTLYCHNCGTKNTPEWRRGPSGPATLCNACGLAYAKKQREEETNLHKLLLHSNSYSYHRGNMLESYSTPSSLLPLFNTAANVPYLHTNSGSSNSTLSYSNPYQSASSSSSSSSSSSSSLKPLSFSSIKSSSSSTGSSERNNNNNNNQNNNISNNKQTTYSFSNPNGGIGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT00</accession>
    <name>A0A8J4PT00_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MIP18 family-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_006231</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MIP18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000278">
        <property type="protein sequence ID" value="KAF2072455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT00"/>
    <dbReference type="OrthoDB" id="2746at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140535">
        <property type="term" value="C:intracellular protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990229">
        <property type="term" value="C:iron-sulfur cluster assembly complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.130:FF:000005">
        <property type="entry name" value="Mitotic spindle-associated mmxd complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039796">
        <property type="entry name" value="MIP18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12377:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR ASSEMBLY COMPONENT 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12377">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR ASSEMBLY COMPONENT 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIP18 family-like" evidence="3">
        <location>
            <begin position="45"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2072455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="163" mass="18448" checksum="D73E717121E0C387" modified="2022-05-25" version="1">MSDNPNPVVYIVDGEKEKQNQLNSLNNESRYTVDEDEIDPFDELEIFDLVRNITDPEHPLTLEQLNVVRIENITIDNEKGTIKLYFTPTVPHCSMANLIGLSIKEKLSRSLPKRFKVDVIVTPGSHSSESTVNKQLNDKERVSAALDKSSNILTIVNESIRIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTB0</accession>
    <name>A0A8J4PTB0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_006102</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000267">
        <property type="protein sequence ID" value="KAF2072579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTB0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="140"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="16"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2072579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="438" mass="51080" checksum="8D0C9B8A727E3FFF" modified="2022-05-25" version="1">MNHQQEEKDYQKDIKEEEEEEEEEESQYDGEDSDEEDGDQEEQEQGNEEQESILLVIKASDNGKKDLYDNLKVHRNASKEEVQLSYDFLFKEYGMDSLTNRDLDEPNHQREKDLLISYFIISHDRFRVIYDDLLDKQQHQQQLENQQHQHLQENNQHIQKQPNSQYKKVSARDRIMGALRFYYGPSFFSVIKHPMTHISVLCQSRPYITDTLKMAKHYLGNFQARNLVEILSLSLFKDSVKHLAILPLSNSVFGTYSPFVSHILTYPLSLVSNLYLMNLGIPLKTIVSNLSFQDLFYGLPLKYTIRFAEAFIAQNLDYLEMFSQMVYVNNPSRYTEFFNKVMASPITKTIVIATLTCPLNVALIQYQSTLLGHICARAGILTPDSAFVLMSSVGLFSFMKNVYNNNNGIRIFYNGFVPFAFSKYFEIKKKNRENKKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTU1</accession>
    <name>A0A8J4PTU1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005187</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000200">
        <property type="protein sequence ID" value="KAF2073510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTU1"/>
    <dbReference type="OrthoDB" id="20552at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053209">
        <property type="entry name" value="Gramillin-biosynth_MTr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47643">
        <property type="entry name" value="TPR DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G12710)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47643:SF2">
        <property type="entry name" value="TPR DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G12710)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="188"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="258"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="490" mass="55441" checksum="D85B017F8E64E3A6" modified="2022-05-25" version="1">MIPQSLKAGSSGEIDFIQGMMRGTRNPMNAPPRDELIRLFKNKRQEIESVCLNRFSTTTTTKSTLTKDLSRKKITELQLVAVRDLLVETIHHGKVLIGRIIAPPFKVNAIQLLLEDQVGGGQHVVQVSIYNYVLPGKKRLGLELDTAFPVGAIVARMWKLFKMLIIHITNEAWKQVLQSKLPKDIDGDDGWRVRGNKYYTDGDYERALDYYNRGLEIDPHHTILAQNKTAALVALKRYQECIKLSLSLISRSTAGVNEKVCARLAKSYYGVGLYQESLETYQQLLTLLPNSSEARTHITKCKERLVELEQGVYNFTSLKSEYKLTKQIDCSEYIGPVKVIHSEEKGRGLVVTQDVPVGTLMMVSHGIGVFIDKMEGKEQQQIIYNVNFRDKQAFDIVHTNIVSLTNSAIKNNPYLGNLVGKLYAGPNYSVSNQIEVDEFRVTKEILSKTPTVPSIEQIQNIVKYNAFKSEVNNSNIMDYIYYLRSSITLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU92</accession>
    <name>A0A8J4PU92_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000626">
        <property type="protein sequence ID" value="KAF2069626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU92"/>
    <dbReference type="OrthoDB" id="23586at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="32"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="223"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="349" mass="40225" checksum="B17607919AF04F62" modified="2022-05-25" version="1" fragment="single">MAEVEEKITTLGEIFYDKYVVHREKVIASDLIQILQDQVTKIDAQNKKLAEEQAKAELQRQIDSMSIAEFESFSVFSDNKEKDQARVKADQYFLEHVQYHIDPKILEILEWKKPELNAERLKIISSDNTKTWYTNYAKLWVVLVADDELKGKIKSKVIKEDLQKMTSSKTYAKQMMLTYNYAYCEIVPKMKLYIRSAEVSKNWAKKAKDYYVSEEYGKIWMTKLHEQQKNEDALNKLRDLLKKYTQTEKLADLIPKDINSLDYVTSNIRAKIDMLDPSGKTSDSVVGYLQNVSLANQILGSGISDEEFNKHLEANVTTIFKGYQELQAPTGTQQPIVKFVKDFAGLPGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUD9</accession>
    <name>A0A8J4PUD9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004968</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000186">
        <property type="protein sequence ID" value="KAF2073735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="22524at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1353" mass="154482" checksum="5CC430EEF27CC0B1" modified="2022-05-25" version="1">MILTDNCINIVPSSYEIKDGILFITQEHAHLFQWSAVLNRENGSDNSNSNGINQLNILVTEQYDHDKYVNHILNIVQQIPDTIATVEITYSHNRNNNNDNNSDINSVSVEAKRIIKLNRSLLDLQYRIQRLLYTLDQIKYLSVPSFTKRRVEFKRDTLNLETRLLNWSIDQEIKHQLVKSSIVQITDTTSSEYLVCLLKRYDDFEQKDIHLELHRYYDGQYDQDILDIFKLLVNQIESIRITVYQIKDEVEQLQETLDASVLKRESLVKRVKEMMPAFVKVVEMKGMDCKMTYFNSLTRGVFYVSSPKHGVIPTTTNHLVWNSFEAPKSIPCGVKRVTFMYVYQSFSGSLIPNTVVSLFLQTYDKEIITDTLPDSITHLGIAKLGVALFKLPAYLKYLSIGKLTIQFSFEKIKVPATLSHLEINDSDQFTHHWLFSKGALPASVTHLTIGEPKINRKLSTPLLIPSTVQVMDLPRHPENFINYKIIDRKDYFQTTSAIPCSDIASIKHIHIDKSFSGPFALSFPSTLESLDLTDYTAKFDKNSLPRSLTSLKCASFRDVPDHARLNYLETKIPNLEVTRVVFKVSDRSITLDQIPTTKNQFLMCCLPMEQLTIASSELKTLSDMLSPDVVIKRLTVISKHALEIPASVQEYEASELPLNYPRSLKRIHLSRRYSYYYLSMDYPETAIYPVPSAVKVLIKNQGNPAKYLQFSDKYSELSLEASKKASHNSLVSVDPFLIIWRNKFLQAKINDYQPQPRYVTFKAEMVNNVRHLVNYTPIDRTRQLKVKVKNLYPVPFGVTSLSIGEHEVKKKVLTAIPSSVTKLKASNYFDTTHDTIPPSVKHLVVTVLEKEIIPPHLETLEIRNRYAKNTLEELKQCLLPTLKRVILSVKCMLNDQFIAQDKLDYLMERKLISGHFYYQGKTPIPPSTITLIWGLNETIPVGVIPHGVERILFCNRFNQTIEKDCIPNSVTEINFGSNFGQCLSQLYLPVSLKYLSLGGYNHPIVPHSLPPNLIQIRIKSFDHFRSLIHLPKSIKYVKFNSMRFQQDHNNKLSSSKVMFKTLYSNPHPHELRKTQDINNSIKLYHQLVSTTTTPTLCPPLDVDLHLDINENLNLVPGLLDHFRVDSMVLGDDFNQCLPKHSLPQSLTKLVFNRLHHSVQETFNLPPNLKYLDLGKVSCSIESLVLPPTLTTVFLPLFGKPVSIPLGYLPHGLKSLHLRADYKNKIEVGVIPHTVEEVSVCQHVDMDNLDFIPPSVTKLSLGVYNANILDCVSSSVTDLHIHPISHNSYSIPANLIPSTITKLSVAQSLKMLNMDELPNQVKELHLSDCIFTGTIPSTVQHIEIKNYNKINQAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUY1</accession>
    <name>A0A8J4PUY1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcription factor IIIC-gamma subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004213</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000145">
        <property type="protein sequence ID" value="KAF2074468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUY1"/>
    <dbReference type="OrthoDB" id="9991317at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039340">
        <property type="entry name" value="Tfc4/TFIIIC-102/Sfc4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23082:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23082">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIIC TFIIIC , POLYPEPTIDE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="514"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="188"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="22"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="53"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="104"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="207"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="949" mass="109319" checksum="E561F05132AE1354" modified="2022-05-25" version="1">MAGKKEKNATKDDKMDVDDDINNNNSSKQKTTTRPITTRTRTKPPFIAKQQQDSDDEVSEDGTDDDYKEEDYLGDDDDDDDVSDDDSDYVDDDDDEDEDDDDYHDEKQVSQKEKEKREQKQKKHDEDYELIRESLKEAAAASKKKSGLYQFNIDSVPFLPSRRRSNKPLTAQEQLQKNISDEVKAMSGKGALDLGDDDDDEDESKKGKGRGPRKIAKHVKKIEAKGNEAYSKGDFKLAFDQFSEVVRLAPQYPVPYKLMARIMEENGDKNGALNFYMYAATFEKTDSNLWSRVGALAMDLKEDKIAIYAYGRAIRNNKADVDAMWNRSQLFIKNKELHKAMNLLRKIETLLGPEEKGNPQIIKAMEDIYAQIEKPEDAPYILEGIVANEMDNSKDIHDMPMDNFNLLLEVFNKTKRFSKATMLFEKLDQKCKASTTPGFNIPIDIIANACVSYFSLNNLKKGQELLDLFMETQNVSEVLDLYLYLGKSLYAIGQISIAIDLYKKMLNDPEGNLPPVWCKIAECYRALKDYRQATVYLHRAHEALPDDIPISFELSELYILAGEKEKSDEILNKTGINSDQKDDPSEKEMYDSTLLNLQERRNSNELKKETVRFQFQHAMAYFNVSKYSQFLGIASALLNGSEDKYSYNKKVVLGSKRGNKRKKDANEYYEEMYQHKVVNFPFAQLLNEEDYFKLIFDTSKVLSYYQRYSEASQYLRYALRNIKFVNPLNAHQLKFLLVGVAFNDKNYVLAFKYVKYVCFKKPYSTKIWNLFNKIILNSHKSTYSNHNRFLLSVSQKYPQSVPVMVLLGNQCKITDNTKGALYEYLRAYKCIPDEPLINLLIGVIVLSQVMGRQHPNRHKVANVSFAFFYKYAKIRGECEETNYNIGRAFHQLGISNMAIHYYELVLNSSEEIDPDHSLKQEAAFNLSLLYKSQGNIALANEILNKYVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PV31</accession>
    <name>A0A8J4PV31_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Kelch repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_004660</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000168">
        <property type="protein sequence ID" value="KAF2074046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PV31"/>
    <dbReference type="OrthoDB" id="10251809at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF18">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="70"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2074046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="442" mass="48736" checksum="E69DCDD592B0EAFE" modified="2022-05-25" version="1">MKWYQTNIEGSIPTVRTSHTSTILNKIDATSSHSCIVIYGGKTQKQTNLVYTDLAFLSVGSGVSVNEEIAKPQNQTSMGKLNKILGSGASSVPSVPVQPPPTQRQPITIGGDRNNSNTASSFTPPAIQKQSPSSSSSSSSASSSSSPSSSSQSRKTYSKLPVYSGSVIYNWENSTSEGDVPGNRFNHSLTALNSGSYKGKIILIGGVINNSNNSNNNLRNELDTINNDIFLLDCDTNYWVKIQAIGIYPEARHSHYVCQISNETILLFGGINDSNQVLNDTHLLNLSNQNTIRWTQPNSMNNAPDLKGKEYKVCFCSGAIYVIISNSNEMYKLDTSTFKWSQVCFVGTLPKFQMEWSSITSYLHYLVFLIENEIYFFDTIGLEWTELDVDGDRPTKRKGHSINVVGSILVVIGGQCEFPLSTIMARDIEVIDLTLFFYKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVJ6</accession>
    <name>A0A8J4PVJ6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004367</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000152">
        <property type="protein sequence ID" value="KAF2074346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVJ6"/>
    <dbReference type="OrthoDB" id="10001977at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="1">
        <location>
            <begin position="5"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="175" mass="20002" checksum="C173035A04370A7A" modified="2022-05-25" version="1">MDCSNLISNDIFTLFDSWVNQSINSYTLLYRATRDGFDSSSFHSTCDNRGPTITLIQSDDGNVFGGYNSQSWNSNDEFYGDNKCFIFTIKNKNNIGPTKYISNKNGDFVYGGVNDGPIFGFHDIRIMKNSNKCQTNYQSFPNTYQDTSGFKGRTFASTFTFKVEEIEIFKIEKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVT5</accession>
    <name>A0A8J4PVT5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003734</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000121">
        <property type="protein sequence ID" value="KAF2074948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVT5"/>
    <dbReference type="OrthoDB" id="418595at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04623">
        <property type="entry name" value="CBS_pair_bac_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044725">
        <property type="entry name" value="CBSX3_CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF10">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="104"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="171"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2074948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="246" mass="27516" checksum="39F4850C11DF6677" modified="2022-05-25" version="1">MLTIKNIFTKSTIRLPILVNQVGLCNLNNPMILGSTTNTTTYNNEHSNKMLMSMVEHKRNNITISPKSVEIIRVKSQIVDQLHPKFNKYKDINVGGIIDKKIASNHGKMVKITETSSVFDAIKLMNEKRVGACIVVDSTSRMSGIFSERDYLSKVDLCGLDPKQTLVKEVMSPKVITVSSDSGANKCISIMTKRNIRHLPVVENNRLIGMLSIGDLVKYVISEQEDELSNLRHDISTNRGNDKSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW67</accession>
    <name>A0A8J4PW67_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="8">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_005550</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="8"/>
    <dbReference type="EMBL" id="AJWJ01000224">
        <property type="protein sequence ID" value="KAF2073134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1712432at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005069">
        <property type="entry name" value="Nucl-diP-sugar_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF30">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03407">
        <property type="entry name" value="Nucleotid_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1 6" id="KW-0853">WD repeat</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBR-type" evidence="10">
        <location>
            <begin position="31"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="1809"/>
            <end position="1848"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="1872"/>
            <end position="1900"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="1901"/>
            <end position="1941"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="1942"/>
            <end position="1981"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="1982"/>
            <end position="2023"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="2073"/>
            <end position="2103"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="7">
        <location>
            <begin position="31"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="143"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="978"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="982"/>
            <end position="1021"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2073134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="2103" mass="244300" checksum="BFCF75662E2E798F" modified="2022-05-25" version="1">MSIKELDQLFVKHVGMNVDEFLTKYPSSNTIKCLYASGDEKNWIASCKNCGVNEEHCFCLSCFLAGDHIKDNHDYSIEKGSYGYSCDCGNTNIVQSGACKQHNNATEQARDNLFNNQLPKDIYDGLYSLFQVLFQYIRENSFIPINEEEEEEEEEEEEEEEEDDEQDSQDQDSNDGNGDNNFFYEENGDFEYDEGPHQSALQGTPEDLLNGAYTLTPEETIVFINQQLVKEKRTINPEIEPIMSWINSISCKSSYVIYIVCEVITGQRMNTYKQYIESYLSNQSIELQDSFLLFLFDNASLLVKLFERYILILLDHCDLMRSMLFVLTMKKNLFIGYDYPFAYSMVLSILSFYNGTHSIVQQHALSPCNHDKNFILSTLNNCLDFLTNIRDNKSSINRQKNRIIGMNFRILGELLKSNIKVVNIFLGNEEMVSKWIQLGMICHHFTDETPQLVYAIPLLIEIQLRSTKMSRYLIKQMPTSKDILLKHLIKSIKQQCLNEIKLMKEQQQQQLQDDNSALPTIQEYLCYFNGEKLKPFSEVQTLYTLLGSIVIQGNVDIKSIDLTDLELVIIFSNIAHLFMSPRRFEIIDSESNEAVSKFSTISTPLDLAVCQICIIKLGSEQFISIYKELLYPMSSQLLFENFLRLLVATLRSRPALVQNDETVRYHTIHGMLSGSMLKTEYLGLKNLYIGIKRAQVKKVYDELKSDKYTIDQLWQEYDPFYCYFSKGYFKGLFMHEKYVKYLQKLDGESSSTTTTTTGSTFHLKKPTIIQDIIPTFNQINQIYNDPDIFKLLLSYYINPKPNVSMVDIQYTLIMCLDMFKSTIYQQLEDATKTSIHQSLQDYFESNDKENYKGFDQYESLTIFSLIKYFDKSCFGLETEQGNPSSTKTSVLDLFISQWKSIQDHFDKAEFLLYFFNWLLEFDHAVFSKVLGANGVDLNKDMKESDTNELAQKKSIQQKQKELVEKMKQQQKLFLQNYGGIMDDSESENENEDEDEDEDDDDDDDDENSNQEDEGDEEDEEEKEKKDSATQFKYSPDTKEHDACVICHSNHTKNGILYKLASVHTWGYNEAMKLRLYTSLANDESIENEAYKTTLDKKKIFFFYNMGNFNRPSLETPLAEYPEFFAKDRAAYISSCHHNAHFNCYKGTQSFDQYSCPLCSKPSNYMIPLDLKPVHLESFYESFQTRGQSLDRSTRDKYLWKYFVHTLDLLELQSRKTSIYCDPNDANDKPYFAISQNTFNKDFATLDLLYNVFMVSNISTNSAGKDFMNYISYFKSDPFHVFSFKGYCFKEFSLESLKESVQHQLFIMFLDEYIKRNDCVNGKDSAPYHSIDMDLNSFKQYYQDLVSKPFPQRLVVDFENKFFPFLRKLAIFSSIVYKQAPMDLELLSDFPKLCQKVLLGMRPFIPTYDIESIFKTLFNPNLLDLYHKDSKAIGKLHMIIPTDYRIGASFIDLEPRLIDLFKKYVSRDMCHKCGDIKDYICLLCSQTHCRTSDHCNHSYDCISVCFTASQSIWEPKLDVIKMGDYQEDLMENHNIYLDQSDDKTFHFFNQYYKQKNIDPCLNLILFNRDNSKDSDDTNNNNDTISNEGIYGEEAESYGNIGFRAICNEKPLVVLDALLKGFNVLWTDIDIVWLNNPFEHIDSLLKQGSNDRLDLITQQDDDDLCAGFYYMKSNTKTIKFIKDVISFLNPVVDDQIGMRLFLKQHGYEVNVNKDKIENNLDKIIFIRLDRKLFPNGTAFFNWKLPQLFNIKPIIVHNNCIIGHRSKKQRFIDYGLWFIDDKDIDIIDKQLLQEKDEQQVLNITEIEPIKILKGHFDVVTSLNSFNNTIISTSMDKSIKLWDYNNSKILKSKYIHKRGAIWSTLVRQETNDDKENNNNTNIITSSHDKSIQIWDTSFSNSIQSFIGHTGLINQIIFIDNNLIASCSDDNTIRVYSLKDENFKRVFIGHCDFVSSICFHNDLLYSCSNDQTIRSWDFNTGRCTNLIRPNQGWIKKIIIDSESNKLFSAGVDGHIKVWELKTLNHLNTIYCFDDLSSISDVVVYDNVLYCSSINGSLKAFDYQGKQIAQYQGHPNSFINCLHISPSLSILLSGGFDKTIRSWKLVNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWN0</accession>
    <name>A0A8J4PWN0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003532</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000112">
        <property type="protein sequence ID" value="KAF2075142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23524:SF1">
        <property type="entry name" value="MRH DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23524">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G04850)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1256"/>
            <end position="1280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1316" mass="145529" checksum="712194ACD08587E7" modified="2022-05-25" version="1">MQYISKIYIIFFILFCTTSNVFSKCPLLVKNVRQGFCDPDLFLMPPVLEIQNTNGLIHSLTPFANVSTMAQPNQGNLMLYYTLEVGTTYTLTYNFTSEVGCVSTEEIVAQGPKLVFEQPKCRYSLGSVDYVGENVLPQPVSFSVDAVTQTLPYQFLAGQGHDFKIEYGDVSCGKTIILYEDTNKGYPLLTVKHDICNGGVGSVILVNHNEYISFALQVQNSPSNLTTINTGQWSSLTAGSYTLLLQSKSCGLQQVPIFIENGFPKFHFTMSPYKGCPSTPLVNIVFDDPTINPIDFAYVYVGTQMTLPFTAPSQYFDLEIRGAQCSTITTLYIPDIVLDVHYTIENASVDGYCGDALFTLVYNETLYTGLSVTDRNNNPVTLTNKQFVGPYNSQFTIRDSCQSNSAIQILAPIPVPIIKTVDKLEKCGDTTDLYIYNYLDFENVTLFLGESSEFLYSDPDGYIRNTTTVQYYVSYYLKGCRVPAYGQGIDQEIQPFNDQVEFLYTITKNPVCNRQGEITFYIHYPPLDLFSKNKTVSFTPDQFINMYHYIEIYGCLVQRGITIDPYRFEAGAPDVIVTQFNDIGCRYMSNASIYIESEFSISYILFKDETGQSPPKAYYPESTISNSTHYGFTYLPFGERDIVIVFGDCSDIVKIQHINLKASSSFELKYSITPITNCQVQDGSITVENPSQFTYLYIYPSRDPAINGVFNNLASDVYHVFIDVASETCYGNVSIHVPTAVQANIHFQTVTNPTCKSESNGAILVFPKSKDDSIFYSVSKLARDDGVTQYGNAFWDLKQGVHNFTVYSMSCSWRASTNVTAEVPSFKYRVLSQTEVGSCDYKTFIQFYSENPNVVISSISQSLNLQGDTLDNIIYGRPSGGTSFFTIFYASSDCSQEIMVENIDYSFDTPDQVPTVTPDCSSSTFGPSLVLDANYNVAFSASGVYLDDFQYDHIPSLENLVYTHFSTGCQANVNAREISTYQPNPVIVNETCYGAVDGTAAVAITGSQIFYQTISPNSNGYSLTFPIQDTSSSLITFSHFTTTGFGIMRTHKDNPYCVDTSLHNVDGFEPSVSLQSTGVCDASNTGSRTEGQIVNELDVEGLNQITYTLNGTSSQSPVFSDNKVGNYTSIVTIDDPRCKRIFKNDVQVAQLSPIGIQVDSSVCLSASFVPAITTVEYQYIITLADSQIESSIHSGKWSLQLPTNGTFSLSVADVTGTCKHFSDFIVNPCPVTPSPSSSSSLSSQSSTEEKKKGVNLGLAVGLPIGLAVLIAALVGAFILYKKRRVNPSKATQFQTEHELTQVSVFTGGKLQKLDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX09</accession>
    <name>A0A8J4PX09_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_003274</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000100">
        <property type="protein sequence ID" value="KAF2075445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX09"/>
    <dbReference type="OrthoDB" id="10264538at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00499">
        <property type="entry name" value="Ribosomal_eL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001380">
        <property type="entry name" value="Ribosomal_eL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018256">
        <property type="entry name" value="Ribosomal_eL13_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01294">
        <property type="entry name" value="Ribosomal_L13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01104">
        <property type="entry name" value="RIBOSOMAL_L13E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2075445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="208" mass="23308" checksum="0CACC58FC53162BC" modified="2022-05-25" version="1">MPIHNKVIQNTHLRKHWQTRVRTWFNQPARKIRRRNTRIAKAASVFPRPIETLKPVVHSASVRYNMKVRSGRGFTVEELKAAGLTPSYAKTIGIAVDFRRTNKSQQSLTANAQRLKDYKSKLVLFPKKVAAPKKGEATKEEVEKAVQNLVVLPVKAGIPVSESKPRKVTEEEKKFQAYATIKFAAAKVKNSGIRKKAAAKKAAAASEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX31</accession>
    <name>A0A8J4PX31_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002285</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the importin beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000061">
        <property type="protein sequence ID" value="KAF2076428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX31"/>
    <dbReference type="OrthoDB" id="2016913at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051345">
        <property type="entry name" value="Importin_beta-like_NTR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363:SF33">
        <property type="entry name" value="IMPORTIN-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363">
        <property type="entry name" value="TRANSPORTIN 3 AND IMPORTIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="6">
        <location>
            <begin position="56"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="648"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="648"/>
            <end position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2076428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1022" mass="116918" checksum="501054A3919F9640" modified="2022-05-25" version="1">MELPQSLKSFDQQQQQQQQQHKNTNDDIEPKPTIDNISQALCALYKSNDPTQINFAQKWLIQFQTLPIAWDIAPLLLCESNIFELQYFGASTIEHKLRNEWSKYNIETKAKILPIIVKIVQNSSNYQTCIVTRICVALSLTVMYTYPHVWENAILDIIHLATQDIYKLDLHNTNNNHYNLENLKLVLEFLSILPTELLKENDQYQNFKQVLKKVIDHVYIFLLSVIKSSNDLEKVKLAFKALASWLKYMLPVTSNLLLCTFQISFDTCLQQEQLLESLPTILEGSAVCLHQRVSADYTEAFNYAMERSLHSLGPIFNKVCQEQDDTKAIPLLNVFIQFLISDEQSIFAKETISRCLEMVLSFISIANREVCSMIFNLLDDYKSHPTLVALEPEKVKFFFLTLLDRFKDLSMYPLDYPSDPDNGDEETDDDLDEDIATFRANTTCCFIFAERNKVVEKTLFHHYLVNQLSQKIGQGVMVWQAYESILYYLYSLSESSNEAQSEFVPILLKLIPSIPVKSTPLIRSSIGLVGKYSFFLKDKSFYLEKVIFDLLPAFNCKPLVATAAESFSSICINNECATILFSHINRIIELCQPVLFGCKVQTNPVVVTIYKALFYIIQTAKSIKEFTPPFQQLVAPIIHNLTQLVNSNNNSNSNSNNSNNSNGNGHHYNNKLNVSNQDKPILLIQLEILASISQMIEFDEFENTTHSDAPYQYFQTIVPLLGKILATYNLDFEALNNIVIIYRWPILFTKNIVTDFLEEILYQVTQSYLQYPFSPLLQVISAMISLTNLPPTFEKKLEYSITSISTLTINQFPKVSNNDNNNNSNNNNIDIPLIDFSTQPDATKDYLFLIMHTLKSRPQCVDRSIVSTLCVSIIYYILDTKDINTTKNCCNFLYGVLSIPSNADLAKSRELVRPVVDNLLQIHGATLINNLIHGLFSGIVSSISLIQFFSDILYSLNNLNEKQFKLDITQIIMNTHTFLPTLHSSDERQNFVSELTTKVNMTEYRSKVRKLVLLANAAKITN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXL1</accession>
    <name>A0A8J4PXL1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003490</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000110">
        <property type="protein sequence ID" value="KAF2075185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXL1"/>
    <dbReference type="OrthoDB" id="19114at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2075185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="89" mass="10075" checksum="ED2870F21DCF590F" modified="2022-05-25" version="1">MSNQNEVDPFYTDSLLVLNGVTKSVKQDLYLDIMESLKAKVNELDSENWAFDINSDTKNNSNNNNSSSSLVSCYNTLYSSHHSTIRHNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXL4</accession>
    <name>A0A8J4PXL4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glucosylceramidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001880</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000048">
        <property type="protein sequence ID" value="KAF2076803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXL4"/>
    <dbReference type="OrthoDB" id="730489at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006775">
        <property type="entry name" value="GH116_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024462">
        <property type="entry name" value="GH116_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052566">
        <property type="entry name" value="Non-lysos_glucosylceramidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12654">
        <property type="entry name" value="BILE ACID BETA-GLUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12654:SF0">
        <property type="entry name" value="NON-LYSOSOMAL GLUCOSYLCERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04685">
        <property type="entry name" value="DUF608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12215">
        <property type="entry name" value="Glyco_hydr_116N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl-hydrolase family 116 N-terminal" evidence="3">
        <location>
            <begin position="415"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl-hydrolase family 116 catalytic region" evidence="2">
        <location>
            <begin position="811"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2076803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1203" mass="136138" checksum="A53B41AF9BB60E2B" modified="2022-05-25" version="1">MEDLFNLNGQDKDIEEYEEILIDAVSPRQSLQSNNMFGTPFSNNNNNNSNNSNYYDNKNTTTNTTATTTYNSNNDSYDDIFFGQTNKGDSKHFSPLSNRFNPRNASTEDINLTFNFDDYGEKTIPPPPLTGSHNNISTSTSSTSSPFLDDTDDQTFSAIDITSSNNNILNNNINISHSNNSINNNSNSNINSNSNSSTVLSPTIPTTTTTTTSTIANNNNSSNNMLSFNFFSSSSTQTTTTTSNTLLSPSIEEVEEKQDSFILPMTSHNNSSNAVQSPPSQNNINSPTTSSTTTTTSIFNLNTLKKAINTTADKLNFSTDRFWNDLGEDHVTADDHSNEFEKWLRLGEDIPHYAWSRRLDQNFADHKQFNVSIWQGLKLMGLGYRMWKYVKKELTAGRLPIMDPFNLPKPGPIMGVPIGGIGSGSINRGWKGDFVRWNLESGIVSNQVVDTDKFSVFINFHDAQYNNVGSITPSKKAVVLYPGKPKTNLNLDVWNWSLKGDNSCYFGLFPRAWTVYEEPHPDIRLICKQVSPVIPHNYKETSYPTGVYEWKIENHNASSDVDISLMLTWQNSIGTESDQAGGHYNKTFSYKEGGSSIKGITLIHKKTQKVDKQVYNDPFELSIAVREDPGLKITYNTRFETTSRLDAANLWYTFNKTGELENTDDSRPSAPKRPIGSAIAVKVTVPAGGSKTIVFSIAWDSPICRFQMGSGYYRRYTKFYGNAGNNSQKIAFDSIQNYKSWDTDISRWQNPILHDPTLPKFYKMALFNELYYFVDGGSVWTNGSVPEIAPSRYPIKHLLKPEDTSDPNSVGRFAYLESLEYLMYNTYDVHFYSSFALISLWPLLEVSLQYDISDSTLIDYNLNWECIHSGSQIPRKAKCAVPHDLGNPGEDPWKRVNAYNIQDVSRWKDLPCKFILQVYRDYLVVQDRNFLLQVYGVVEEVIRRSFEEFDKDKDGVIDNEGFPDQTYDTWSAIGCSAYSGGLWLASLKAASEMAKVLGFKEDQEVYDRIFENGSKSYNKKLWNGHYFNYDSSKSVHANSIMSDMLAGHWYLISCGLESYITFDQALSSLSIINEYNVKSYGKEICGAVNGMRPEGMVDTTCLQSAEVWIGTSFSLAATMLLHRMDKEAWDLVEGIVNSSYQKWGFQYQTPEAWDLNGQYRAAAYMRPLSIWSIQWAIEKRKDFDYYTEYSKTQPQNSRESLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYJ1</accession>
    <name>A0A8J4PYJ1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glycoside hydrolase family 28 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_003381</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000105">
        <property type="protein sequence ID" value="KAF2075304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYJ1"/>
    <dbReference type="OrthoDB" id="187139at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046576">
        <property type="term" value="F:rhamnogalacturonan alpha-L-rhamnopyranosyl-(1-&gt;4)-alpha-D-galactopyranosyluronide lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31736">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31736:SF19">
        <property type="entry name" value="PECTIN LYASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035239340" description="Glycoside hydrolase family 28 protein" evidence="7">
        <location>
            <begin position="20"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2075304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="415" mass="46266" checksum="7609F044B25B6354" modified="2022-05-25" version="1">MRKKILLIYILAFITCSLGDLIVYQGIPLDSSLETAWKNGGLLNETLNQLNSGDELVFPNTTIYLVGGIVAEGLEDIVFRFEGTLVFTDNTHTWPKNASGNVLECFQFSNFNNVTFTSSQGGILNGQGEAWWGLIGYVEYKENRPRMLSLGDSKQILIENLSFINSPYWTVWIFNVDGLEIRYSHITNRRNDFDGHDDYNMEAFNTDGFDVTGNNVWIHDCSVWNQDDCIAVKDGSSNMLIERVSASGVGLTIGSIGGSDVRNITFRDCYMHKTYKGLYLKFRGSGSITDVLYENIVIFEPQTYPIWIGPAQQADNVDICLAAPCSLCWPSIPFAQCNMPQNASYIDITLRNISIIDPVDQTGVIMGSPSNPMINITFDNVIFQNPKSNYYSCENVATGYATGSTTPTPPCFQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYK7</accession>
    <name>A0A8J4PYK7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dihydroxyacetone kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_002567</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the dihydroxyacetone kinase (DAK) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000072">
        <property type="protein sequence ID" value="KAF2076105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYK7"/>
    <dbReference type="OrthoDB" id="1724672at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004371">
        <property type="term" value="F:glycerone kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1180.20:FF:000001">
        <property type="entry name" value="Dihydroxyacetone kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10440:FF:000001">
        <property type="entry name" value="Dihydroxyacetone kinase, DhaK subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.340:FF:000002">
        <property type="entry name" value="Dihydroxyacetone kinase, L subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10440">
        <property type="entry name" value="Dihydroxyacetone kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1180.20">
        <property type="entry name" value="Dihydroxyacetone kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004006">
        <property type="entry name" value="DhaK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004007">
        <property type="entry name" value="DhaL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036117">
        <property type="entry name" value="DhaL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050861">
        <property type="entry name" value="Dihydroxyacetone_Kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011049">
        <property type="entry name" value="PRK14479.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28629">
        <property type="entry name" value="TRIOKINASE/FMN CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28629:SF4">
        <property type="entry name" value="TRIOKINASE_FMN CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02733">
        <property type="entry name" value="Dak1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02734">
        <property type="entry name" value="Dak2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01120">
        <property type="entry name" value="Dak2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82549">
        <property type="entry name" value="DAK1/DegV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101473">
        <property type="entry name" value="DhaL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51481">
        <property type="entry name" value="DHAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51480">
        <property type="entry name" value="DHAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DhaK" evidence="7">
        <location>
            <begin position="7"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="DhaL" evidence="6">
        <location>
            <begin position="396"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2076105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="623" mass="67866" checksum="7824C702EFC6CA8F" modified="2022-05-25" version="1">MKKLINNVDGIVDDMIDSLVLSNQHLKRVDQYHVVVRADCGEKVNDNKVKLISGGGSGHEPCHAGFVGRGMLCAAVLGDVFVSPSPKHIVEAIKAVANHSQQARDTGCLLIVKNYTGDRLNFGIAKEIAVNEYGIAVDMVVVDDDVSVLMNDSVVNKDTKRRGIAGTPLVHKVLGALAERGNSLHDIVQYYHKYLCPTASNNLATLGVALSSCTIPAIGNPTFEIKEQEIEFGLGIHGEPGAYTTKMTTSKEIVKNILDHLVKYIDNSNDKDIVVLVNNLGSTSTMEQMIVVKDTIEYLHGLDYKIHRLLSGHFVTSLEMSGISISILSIKDKDTLTLLDSNTTATAWPINGLLCPIAKGESVNLKYNNQSTTSSTSTDDKYSKLKSIKVSKQHGELIKGLVHRCCKDLIDSNQLLTDLDSKVGDGDLGTTMERASNGVLKEIDSIPFEHPCFAFRVLSNILSDTLGGSTGPFYSIFFMKLANTMYQRCTSNGTNEPQPMDWGLALVEGQNAIQELGKSKKGDCTMLDALVPAIESIESSVSKQQHDTITLVQIFEEAYKASEQGAQSTIDMISKMGRSSYLGDRSKNVMDPGACCISIIFKSLFNSIKEYNNTNKVNSELLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYM4</accession>
    <name>A0A8J4PYM4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_003738</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000121">
        <property type="protein sequence ID" value="KAF2074952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYM4"/>
    <dbReference type="OrthoDB" id="18054at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030587">
        <property type="term" value="P:sorocarp development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000335">
        <property type="entry name" value="ATP binding cassette subfamily A member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="255"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="299"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="339"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="397"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="431"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="524"/>
            <end position="759"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2074952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="842" mass="96686" checksum="819E4FBFD926E901" modified="2022-05-25" version="1">MIDKRNYIFIQFKAILKKNFLLQFKQVKSNVIQLVFIICVMVLLIYTSRDIVGGYDRYEYNRYIDFDYYITNSAQEYPYNQILSKYWAVDPPQSSSKVTAKDILGNLPQYVDKETNITVPIVEYFPYADYNQMDSKMITFWNDTFSYWKNIAFKNVTSDLSKVPPYYTIYFNTVEDSNGGKKKLNYTIENLYQELQSVYYQSLFLESRKFNEAYTLISFINNAYLKYLTPKEDNFIWAVKQQYIDRFTSERQNTFSFVLCIVLLPPVVSMVFTTFVHSLVTERAELHMSLMNLMGLKTIVYMASNAVYFFCVFMIFVVIVMLLGTIGLVPLFTGEFFRLLVLFISYGLSLSSLSFSVSAFFSSTKMSSVLSYILILITPLVGSLLDLYFYSGHLLSAIYLVFPPFTFQHGMYLLCVHLADHYEKFPINSQFTAVIFALLFQSIAFFLLGIYLTNVLPKKFGYSRSPLYPIKGLIHYINNRFLKRQNQYSNFDSDKEIEIEITDEDYDCKEERRRAYDQDNNYILRSVNLSKTYKTGKINKQALSNFCLTGQRGEILALLGPNGSGKTTFLNLLCGVHRPTSGDAFINGLRISTEMESIYSIVSICFQKDILYEELTVLQHMEFFFGIKKTSIGATSTSDLIKKILEGVLLYDELNAKAKNLSGGQRRRLSIAISLIGDSKILLLDEPTTGVAPDSKRTCWDVIQSIKSDKLIILTTHSLEEANELADKLAIISSGQLQCIGTSTHLKHRFGSGFSLDIILYAPEYKLQLIDSVLSQVPSAQPKETIIPDEATFIIPNQTDIIKLFTILNEKKEEYGIKSWSVSHSSLDDVFDQIVNQREEIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYQ0</accession>
    <name>A0A8J4PYQ0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_004171</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the copine family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000142">
        <property type="protein sequence ID" value="KAF2074544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYQ0"/>
    <dbReference type="OrthoDB" id="5855668at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04048">
        <property type="entry name" value="C2A_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04047">
        <property type="entry name" value="C2B_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000219">
        <property type="entry name" value="Copine-E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037768">
        <property type="entry name" value="C2B_Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF106">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="4">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="4">
        <location>
            <begin position="122"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2074544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="526" mass="58246" checksum="CD3DBDE3CA530686" modified="2022-05-25" version="1">MTTTHIPTSRIELRFKCTKLANLDVLSKSDPQIYVSEKRGNRPLTYIGKTEKIKNNLNPIFATPILIDYYFEESQDLRISVVDVDGEISSADHDDPIGDLSITLGNIVASQSRRVVKDIKNKHGKVTGSIEITFEEIAKTGHNLLLRLGGLNLDKKDLFGKSDPYYKIFKDTANGFVEVYKSEYIKNTLNPVWKEMVIPLENLNGGDMKRPIQIQVFDYDSIGDHDLIGIFSTTMDEVLLGKKEFPLINPKKTTKSNYKNSGFVSFYDARLVKDFSFLDFLYGGCEISLIVGIDCTGSNGEPTSSTSLHYKHPTKPNEYAQAIVSVGSVLTPYDTDGLIGVYGFGASLPNSYATDHCFPMTLTQNPEVLGVPGVLDTYYNNITKVSFSGPTYFAPLIDKAAKIAAKGQSQEQQKYTILMIITDGEITDMDNTIEAIVKASVLPLSIVIVGVGLDSFSSMVTLDGDDGPLVSKGVRAERDIVQFVPLRNYASNPQELASETLKEIPGQLLSYMKKMNYHPNTPKMPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYT1</accession>
    <name>A0A8J4PYT1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA mismatch repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_002216</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the post-replicative DNA mismatch repair system (MMR).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutS family. MSH3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000059">
        <property type="protein sequence ID" value="KAF2076476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYT1"/>
    <dbReference type="OrthoDB" id="10252754at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032301">
        <property type="term" value="C:MutSalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.10:FF:000004">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1420.10:FF:000004">
        <property type="entry name" value="DNA mismatch repair protein Msh3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003810">
        <property type="entry name" value="PRK05399.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF153">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="DNA repair protein MutS, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="9">
        <location>
            <begin position="1096"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="298"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="53"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="99"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="119"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="184"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="198"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="323"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR037677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2076476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1267" mass="143821" checksum="F80A0BB3EE15C94E" modified="2022-05-25" version="1">MPRLKLPKKLEEELKAQQEQEQQEQREEDQDEIKKTPAKRGRAAAKPAAPVASTTGPKQTSISSFFTKIPDTKSSTVDKDGDDDKDDEKHVTAPISKSTTTTTTTTTTTTTTRKATPKIQDKGTDTSISKESKSKATTTSRKQKEQENKAAQLDESPTTRRARTNVKLAFLESDEEEEEEMEKKKKKPMNRKRKKDESDYESESEVDDSDSDYMSQDDVSDSDTVSERDDNDNDDLDILNIDKFDKMDESKDSFFDDSAPQIVLYDGKVVLPKGTPDFQSNPKAGQKLLKAHDTLQKMKEDQTNKAPEGARDDDDEEEEEKPKKKTTAAKGKSKGPSYTPLELQFIEIKKKHPDTVLMVECGYKYRFFGEDAEIANKTLNIYSYVAKNFLNASIPCQRLYFHLRRLVHAGYKVGVVEQTETAALKAISTSKSQPFERKLTRVYTSSTFIDDTVDDTLSASDSSPNYMVSFYESYSNEDDPQISFIAVSIRTGEIIYDTFKDNLMRGQLETILTHLKPSEILIPPSLEKVVTQQQQQQQAKDVGADFKFSKLTSKVLKNYCKTHHVRTQVMSADLFNHDKSLLALMQFYESCGRESTLTSLQSFLKPNQIICLGVLLVYLKQFVEFCNILQMEDRFKPFRLNNHLVLPHSTIENLEVLYNESDHTERGSLFWILNHTQTFSGRRLFSNWLCKPLNNLELIIERQEAVSELVESIKGIRKQSQASVEKITQLLKSHIPDLQKNISRVYYKSQCTPKDFVNTMESFKKIVSVFSQISSHEPPVFGAKLLNSIFTMEKDQQISDRVRYFLENINIDKAKENFIVGADKSNLWITVDKYPKIKEAQDQIHTINNELNKYLKSIKVELGKPTLEYVHMPKLNAEFLIELPISFKNIPKNWFKVNSTQKLVRYHTPIIVEQIKLLSQNREKLKLSAQEAWISFLNEFNNDYILFSNFITKLSNLDCLMSLAKVSSFDGYVKPVFKTDKGVNGIEIIDGRHPVVEVLRDYVPNSICLSSDKETSMIITGPNMGGKSSFIRMTSLLVIMSQIGCYVPAKSCVLGGVFDAIYTRMGARDHIEKGQSTFFVEIQETSDILKLATPNSLVILDELGRGTSTHDGVAIAYATLKYIVEKVKCFCLFVTHYPLLAQLENQYPKQIGNYHMGFIEEKDVVVDGNQDTSIPKVIFLYKVEKGAAQNSYGLNVANLADIPKEILIEASKKSNEMKESIVSKAKLDIDTSATINDKTKQLVQSVKSVMKSKVDIIGKLKQLQINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYZ6</accession>
    <name>A0A8J4PYZ6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">fructokinase</fullName>
            <ecNumber evidence="11">2.7.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_006793</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ROK (NagC/XylR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.4" evidence="11"/>
    <dbReference type="EMBL" id="AJWJ01000329">
        <property type="protein sequence ID" value="KAF2071891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYZ6"/>
    <dbReference type="OrthoDB" id="10260668at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008865">
        <property type="term" value="F:fructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd24067">
        <property type="entry name" value="ASKHA_NBD_ROK_BsFRK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000136">
        <property type="entry name" value="Putative fructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000153">
        <property type="entry name" value="Putative fructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051804">
        <property type="entry name" value="Carb_Metab_Reg_Kinase/Isom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049874">
        <property type="entry name" value="ROK_cs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42742:SF3">
        <property type="entry name" value="FRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42742">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR MPRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01125">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2071891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="304" mass="33013" checksum="389BE0E4EC67F6EC" modified="2022-05-25" version="1">MQNNLLFAGIEAGGTGFSLAIASGQPSNIIEKTHINTTTPEETRENVLNWLRGKEFNAIGLASFGPVDLDKDSATYGYITTTPKPKWGNTDILGWFKEFDVPKGFDTDVNGAAISETFHGLHKRGPITSCAYITVGTGVGVGVVVNETPIHGLVHPEGGHTFARILPNDKSEGTCPFHKNCIEGLVSTGAISKRLNLTNDQLKNVGDEDPVWTTIGHYLAQLCANITLLISPQVIVLGGGVFNRKILYDIVRKETINILNGYIKSKYLTIEYIDQYIVQSPFDNNAGLVGSLELARRAILNQSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ29</accession>
    <name>A0A8J4PZ29_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Rab GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_002070</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000054">
        <property type="protein sequence ID" value="KAF2076641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ29"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000274">
        <property type="entry name" value="ras-related protein RABA5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="196"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="201"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2076641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="225" mass="25117" checksum="D1CE6957A5C00B8A" modified="2022-05-25" version="1">MPEEESEYIFKIVIIGDSAVGKSNLLNRFTRNEFTDKTKATIGVDFGTKSIEIDGAVVTAQCWDTAGQERFRAVTSGYYRGAVGAMIVYDITSKISFKNVTRWLNELREMAEQDILIMMVGNKSDLEMSREVPTKEAQAFAESNKISFLETSALNSTNVNQAFERLLTDIYHQLSLSRKPVVADDVQNWLVPNQGKKLTPITDQPTSTNLASTTSTHQEKKGCCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ32</accession>
    <name>A0A8J4PZ32_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase S54 rhomboid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_003870</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000127">
        <property type="protein sequence ID" value="KAF2074844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ32"/>
    <dbReference type="OrthoDB" id="418595at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050925">
        <property type="entry name" value="Rhomboid_protease_S54"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731:SF32">
        <property type="entry name" value="PEPTIDASE S54 RHOMBOID DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731">
        <property type="entry name" value="RHOMBOID PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="7">
        <location>
            <begin position="171"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="90"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2074844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="329" mass="37327" checksum="F61BA94624B9FEDD" modified="2022-05-25" version="1">MNTRFVSNLLRNHFKKINNNSGAFFKINGHSSNIAFQPSSIFNSKPNVSMVLNDCNKNNHTIYSCSFSSSSSDSNKSKFKLTLKKLQNSKRDYNKKKQEQDQWYDEQETQNNSFEQDREYYSSAKTSNSLIYGLIAINVGTFLFINSDDSYQFQRMIMDNFTLSTETFAKQPLTLLTSFFAHKDIYHLLFNMLGLYTIGTSLLYSIGTGPFLTLYLGAGIAGGLGYLALQKYYNTNNNRTLYRKRPVGALGASGAIYGVFSTYALLYPTSTLMIYGIVPVPALLLLGGYMAWDFYNEYYHKNTGIAHSGHLCGGLFGGLYYLTKLRRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZG7</accession>
    <name>A0A8J4PZG7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Rab GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002866</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000084">
        <property type="protein sequence ID" value="KAF2075832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZG7"/>
    <dbReference type="OrthoDB" id="63533at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000808">
        <property type="entry name" value="Small GTP-binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="178"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="22950" checksum="68F935F06C115BD2" modified="2022-05-25" version="1">MSYLRNYPQVIVPVKIVIIGTANAGKTSLALRLVKDTFQNESLPTVGAMFLNKFFAFDNYTIKFEIWDTAGQEKFHSLANMYFRGSKIAVVVFDLSDTDSFNKAKIWTREFKTNSNPEEPAIMALVGNKCDLNKATPQSEIDAYVEQEGMLYFEASAKENTNVNNIFRTLADKIPAPQLKQQQQQQQDTIDINKNNKKKKCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0Z0</accession>
    <name>A0A8J4Q0Z0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006164</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000272">
        <property type="protein sequence ID" value="KAF2072521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0Z0"/>
    <dbReference type="OrthoDB" id="16995at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="97" mass="10576" checksum="A68AD3FB7569633F" modified="2022-05-25" version="1">MSSLIDLDFETTTESPSPKVLPLGTESLISYESPKPTTKSKQNNNSNNNNSNNSNDNDLFSDLGIKDHTIQETSNAKPLSDSDIMKKFSSPPTKATI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q120</accession>
    <name>A0A8J4Q120_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_001862</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000047">
        <property type="protein sequence ID" value="KAF2076834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q120"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03750">
        <property type="entry name" value="proteasome_alpha_type_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000012">
        <property type="entry name" value="Proteasome subunit alpha type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 4 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="6">
        <location>
            <begin position="5"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2076834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="232" mass="25680" checksum="1E208C452E360B03" modified="2022-05-25" version="1">MDSDYSFSLTTFSPSGKLVQIEHALQAAASGGSAIGIKAKNGVVLVTEKKLHSLVDVSSVQKIAMITENIGLVYAGMGPDSRVLIKKARKESEKYYKQYHEKIPVLQLVRELASIMQEYTQSGGVRPFGVSLLVSGYDEKGPHLYQVDPSGSYFAWKATAIGKNMVNSKTFLEKRYSDDLEIEDAIQTALNTLKEGFETQMTETNMELAIIGKNQKFEILSPSQIKDYLLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q138</accession>
    <name>A0A8J4Q138_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001918</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000049">
        <property type="protein sequence ID" value="KAF2076792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="29720at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051014">
        <property type="term" value="P:actin filament severing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008154">
        <property type="term" value="P:actin polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.950.10">
        <property type="entry name" value="Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007122">
        <property type="entry name" value="Villin/Gelsolin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003128">
        <property type="entry name" value="Villin_headpiece"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036886">
        <property type="entry name" value="Villin_headpiece_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977:SF51">
        <property type="entry name" value="PROTEIN FLIGHTLESS-1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977">
        <property type="entry name" value="VILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02209">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00597">
        <property type="entry name" value="GELSOLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00262">
        <property type="entry name" value="GEL"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00153">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47050">
        <property type="entry name" value="VHP, Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51089">
        <property type="entry name" value="HP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HP" evidence="3">
        <location>
            <begin position="1479"/>
            <end position="1539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="133"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="276"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="678"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="731"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="755"/>
            <end position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2076792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1539" mass="177371" checksum="E7E1125AA2013F33" modified="2022-05-25" version="1">MDNVDEENEKRRKRLEELKQQSLDIEREAQEREKKNEERKKQREEQLRQLQEQEKKDEEERIKRREERKRLREEEEKRFKEEMERLDAEKANRANRLNNGADSPLSSDPTSPVNGSSEPISSSKSSSFTLDDPELRKQQEEREKRIEERRKQREETERQLALEEKRIAEEREKRKEERERERLERERASENEKLQKAASSESERLEKQRLEVERLEKERLEKEKAAQEAKDKAAESERLEQQRLEVERLEKEKLEKEKAAQEAKDKAAAAAAAAAEAERIEKEKLEKEKADKEKAEQEAKEKERLEKEKADKEKADKEKAEQERIAKEKADKEEKERLDKEKAEQERIAKEKSDKEKADKEKAEQERVAKEKADKEKAEQERIAKEKADKEKADKEKAEQERIAKEKADKEKADKEKAEQDRIAKEKADKEKADKEKAEQERIAKEKADKEKAEQDRVAKEKADKEKADKEKAEQDRIAKEKADKEKAEQDRVAKEKADKEKADKEKVEQERIAKEKADKEKAEQERIAKEKADKEKADKEKAEQERVAKEKADKEKADKEKAEQDRIAKEKADKEKADKEKAEQERIAKEKADKEKADKERLDKIAKDKADKEKAEQERIAKEKSDKEKADKERIAKEKADKERIEKEKQQQKSETSEEKKSLDTSKITDVFKSSIEKQLQSQLEKKNTSLVSTEPTSLSSPLDHNMALDKARIKGRKPPTRGVGHIHASSGGSSPSSSTSNSNDNSPSLLSQALENKPSTPESTKKKLVLPPGAQGGLLGGMAALAMEAQKKKLEKDNNKLLQAVKDEAPSFVRGRSQSVSVSSTLKQEPGKFLSHKKTLDTYSGLRTRLIHCKGKKRICTKEVEVSINSLNKCDAFVLDCGIEGSNVGGESVDSNSHSTIYTWYGSKASANKKAKAVAIAEIIKSHERGGHATITKLDEGDDDPLFYKKLGGTVKSNINPEGGDDLEAETHWAQSFTLLKYNSDTDSLVHVDTKSLSMEVLHSDGFFVLDTGTEFYEWSGKNSDQNLKENFHKKAQERLKKDTTHRQAWVEAVITSEGGENVLFREKFFDWPDLSHEVSLSRMGFGKKKAFDVAIPYEKRTPTKMNNFEVREMVEIERAEEELKSTGNGEYEIWYVDNMKIHPLPKEEYGHFYSGNCYLLKYTYTKWNALKYIIFYWQGIDSIRQDIGASSLLSKDMYIETSAKGDCVQECERMGRESTHFLSIFNGRMVVHRGTRTDFDMKSKRLYHVFGRKHNTIVAIQSTRLSSTALNSRDVFIATDSVNTFVWESKGHSKALKEESSKLASLIGGKVQNIKESSEPEAFWKLIGGNGKYANYDYIHNHLKPKDFQNEIKLFAIINTGTIIRAEEIYKYSQYDLTPSKVFILDNKKNVFVWVGSKSQEKEKKRGTEIAIDYLKYLNDSRTESDVLFINEKDEPLSFTCFFHAWDSLRFHSPNGADLLEDITSPKVEAATSVLKKYYQVLPFEQLIQKNTPPEIDRAVLEMYLSDEDFEKHLGMNRSEWDALPAWKRTEKEKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q162</accession>
    <name>A0A8J4Q162_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Short-chain dehydrogenase/reductase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_002497</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000069">
        <property type="protein sequence ID" value="KAF2076197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q162"/>
    <dbReference type="OrthoDB" id="13950at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05374">
        <property type="entry name" value="17beta-HSD-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051911">
        <property type="entry name" value="SDR_oxidoreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43976">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43976:SF16">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2076197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="298" mass="32656" checksum="172BD3EEEC2488CB" modified="2022-05-25" version="1">MSTTISSTNTSNENNNIVWYVTGASKGLGLELVKKLVQDGFKVVATTRSKQLLIDNLGQHYNAEQVLPLVVDLLNEESIKQSISDSLAKFAKIDIVVNNAGYALMGAFESITDQEARSQFDVNVFGVFNVIRNVLPHLRNNKTTEHGPHIFNISSIAGFSGAFPNGSVYGATKFALDGVTQHLQTELKPFGINVTSVLPGVFRTEILDPKSMVVAKQNPDYSSSQEFVNQFSQLLNSDKVGDPSKLAALLIKVSKEKNPPLTLPIGPDSFGLFEKRIQDIQKDLIDYKDESTSTNFSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1V4</accession>
    <name>A0A8J4Q1V4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">IPT/TIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_001458</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000034">
        <property type="protein sequence ID" value="KAF2077269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1V4"/>
    <dbReference type="OrthoDB" id="5951731at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052014">
        <property type="entry name" value="Dictyostelium_Tiger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31341">
        <property type="entry name" value="IPT/TIG DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035171305" description="IPT/TIG domain-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="4">
        <location>
            <begin position="265"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="4">
        <location>
            <begin position="389"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2077269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="551" mass="60066" checksum="D10FC869B9DFD19E" modified="2022-05-25" version="1" fragment="single">MFKTSVVYLIIGLVLSLLCRVDAAGVPIIQTISIKESIVIVSVNTIDPVTKITLDTFDCEITSNINNIVQCTPPKEFFRLGSFEVKVENQNGMTTLDALYQNLINSYASDSSKQGIFKGSFEISNTVKIYGIDSTPISIYSWTDETLSFKYPDTIKCGYAFLMDSGDRQSNDILFCPNPFIASFSVTNNLLRLVAQYLQPTPLDTFLLFVLYPGDSTEYPCTSAPNGFGQLNVDCSLQQSKRVYSFNAYYVNTGRSLTFPLYNNPSISSVVPTAYGHPGRVTVFGQNLTPKIGDITVQIGSGDCSIISVATDKIVCLFQSDIPDPSPNSLTVSVIIDTLYVAHRDIFEYSCPRDDNGLYCSSHGYCQLSTLTCGCDKYWDSFDCSIIYPQLTSISSTHYGEPSFVTIKGDSFYNSKLNITIGGSPCSSPVVVDPTTIVCMFQSNVQQNAQLDPLVVFLSIETKNTVEKPLFSYSCRVGSNGKICSGHGLCKDNMACVCDKGWGSTVDCSTISPNIISSTSTKFGSPSNVTISGDNFAPIDLKVKIGGLFCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1X4</accession>
    <name>A0A8J4Q1X4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">DOMON domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_001424</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000033">
        <property type="protein sequence ID" value="KAF2077299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1X4"/>
    <dbReference type="OrthoDB" id="19261at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140575">
        <property type="term" value="F:transmembrane monodehydroascorbate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08760">
        <property type="entry name" value="Cyt_b561_FRRS1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09631">
        <property type="entry name" value="DOMON_DOH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045150">
        <property type="entry name" value="CYB561D1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006593">
        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045266">
        <property type="entry name" value="DOH_DOMON"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005018">
        <property type="entry name" value="DOMON_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15422:SF24">
        <property type="entry name" value="DOMON RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15422">
        <property type="entry name" value="OS05G0565100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03188">
        <property type="entry name" value="Cytochrom_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03351">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00665">
        <property type="entry name" value="B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00664">
        <property type="entry name" value="DoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50939">
        <property type="entry name" value="CYTOCHROME_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50836">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035290204" description="DOMON domain-containing protein" evidence="12">
        <location>
            <begin position="22"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="242"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="276"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="320"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="344"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="DOMON" evidence="13">
        <location>
            <begin position="33"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b561" evidence="14">
        <location>
            <begin position="173"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2077299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="375" mass="42530" checksum="AF93781A96C338D5" modified="2022-05-25" version="1">MMLKIYIFILSIFYLIHYSQSVLSPGVILDPINNFTLKWDIINNDTIVFQVEVNVKTWIGIGWHPSNRESDGPMINSDYAVGMFDNVTGLLKVVDMVSGAKAPGKPVPDSSVNATNDFMTFSGYQQDNYTFMVFARKLITNDTVGDRDIYLDSPLDLIWAYGTSNNFGYHKTNAGRVKVSLWNTNPLANQKETQALVMVVSSDSKNYKTWHGALMMISFGVLMPLAIFVARYLKEYSWWFPLHIFLQTGAFICIIVSFALIIVQKTRLDLSIAHNIIGFIVILNLFIAMALGVCSHFMWNRDRKKTPIFPDIVHHYHGRFVFLLSVAAMITGILKYSPSPLSNPFVVCYSMIIGLYLAVFLFIEVYKKFISSTPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q200</accession>
    <name>A0A8J4Q200_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000058">
        <property type="protein sequence ID" value="KAF2076494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q200"/>
    <dbReference type="OrthoDB" id="19902at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040310">
        <property type="entry name" value="DDB_G0292248"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31648">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25544">
        <property type="entry name" value="Ependymin_amoebozoa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035301828" evidence="1">
        <location>
            <begin position="20"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="221" mass="24113" checksum="267CCA0441837CB5" modified="2022-05-25" version="1">MKFISIALLLTLSVCFSFAALCSLNNGYSSQYSVNSYNTYFPTESGYEASTFFESGMVTVDYDNQMMNVISEVLSNGKKIVSQIYAFNGNKTLYVISGTSSGGMVCNTFPLKYPVPTSAPKVLNDLGGDYIGDIPVELLQVQSNNGPNVVERTLFDFFTCSPTVSFITNTVKANPGIATMVFTNYGNYSIPIELDSSCYNAKPGDESMVHLPKAILSHPYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2R8</accession>
    <name>A0A8J4Q2R8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_001735</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000043">
        <property type="protein sequence ID" value="KAF2076959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="23837at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035158091" description="EGF-like domain-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1343"/>
            <end position="1365"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="7">
        <location>
            <begin position="432"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="6">
        <location>
            <begin position="670"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="5">
        <location>
            <begin position="809"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="4">
        <location>
            <begin position="1101"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1025"/>
            <end position="1046"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2076959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1384" mass="156278" checksum="9388B0F2A3D00A32" modified="2022-05-25" version="1">MNRYRSISIVLLSLFLLYHHTIGILGLDLEVEDVTSRFLVGSNDEYSEIAGQPYCSVQYYFKIKNNVPESKYVDISTNSFSSTKASFGSKTLVTVFYHITRGTSGSIDVIVNTNISSFTFPALVSYNCLDTPFPVDPVYNADLYPHQSIDGTYSLLSKYKNYVKYSPVLTTVDQNVPLIAQMLYLNYPLFKINLNINPILSFSTKDINMQNTTISINNFLNSNLGIDSLSTQNNNYYPYNCENFNYFHCYQMFDIVTNSPNYFLGVYSDMADLSTRYLLPIFGNPLNATYFGFLTNDKQFTIVKWNGAQADFDILFPVMSFYRDDQPILNWPNNGLPQIGTLPTPSYHFQLLSPYLSTWVRVTLYTSDFQGQVHRAPAPYGFYSGNLTQGILYNFDVPIYDGQSEIFFTLVNTDDFYYNKRDGSLVATPLIPDTIYPVLKYARFVDLGNGMVSLQIHATDIGSGVSCFVFRDQDILMDTRHLKEGTIFDGVFEIILPKSYWTQQPLQLSLFVLDFAANREDYDSDKILNENMESFPRNPYAFITTYRLIDNIIDIAFEKNDVDLSQSAVNNSLVVKLNPPLPHTTLSLIPCFKLHFTNDFLTGGVPYLDKDTEFNSYFDIETQSHIINFTLPSRLFTGVFSYKLLPFDLDNTILYQLNPKAELRYFSENADVFPPIVTSIVQYPSPIATIGQDTTIGFEITIEDKINGFKSGYAIVTSELDILGYNFTLTSSIDNPNNVFQLYFPLDVKSRPQTFTISKMYLEDNNGHFSFFPSIQDINPLMNFLGDTETSTLSTQFIDPLTLNDTTVPVLNTFTIISNTTDESQEFFIRTLIFEYEIYDDDSGVSTIHLPKLFLTNAQFEFAESNSTIVKSRSPDGKTIVFQTTINYSLYFAFNHNPLISLHGIYDNHMNLVGFSSKDLNSKSFVYCVERITSIPLPVINEIQVENNALILNGFRLGASNSLNTKLDILDPVSNVKVSSTATFIHQNSNQIKIDLNLIPINSTMNLQLSILNILSNRIYFDNTPQDSSSDSSPTSTTSSSSDETNSNSFEIKKVCTTDKDCNGIDNGKCINNACICKYPAFGVNCFDKVVPDSKNDFNTTQPSTSITVNNDQLNLKALVSIVKIIEYDFKKQSVFEFIFNKWEYNQTTKSNGNQLHHYKTNITKDTRDSLISVDIESFVQGENITFAKQELEMVPNSNKYSIHLANYPFSKSTNYLQLIMSVSLGIDVQDSCSHKESGDIVTNSYSEYFKIQVNDNSLYGRFIKRGIIDGKVTVVSNEITNNADSQDTSNLVGSFIGMNIPFFQKTAILDPDFSLLISTPVTNNQENSICSPKKNKLSAAKIAGIVIGSVGFATIVVVSTIYYFQKKKQSLQLQKSLSLRNIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3E9</accession>
    <name>A0A8J4Q3E9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pmp22 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_005304</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000207">
        <property type="protein sequence ID" value="KAF2073391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3E9"/>
    <dbReference type="OrthoDB" id="16786at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF17">
        <property type="entry name" value="PROTEIN MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2073391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="226" mass="25862" checksum="ED68B8AE3100E213" modified="2022-05-25" version="1">MKVFFNAWRLYNNALQTHPVKTKAITGGFVFAAGDFLAQRLENKGEYKIERTIMMSTVGMFVIVPQIHFWFKFLDKIVPSKSIKATCGKVIIDQVFGCPWFVFCNMTSVQILSQRNNFDVEQWKTKISENMLPVLRQAWTIWPITNFILFKYVQQDYRLILSNIVSVGWNCLLSTVANKNINDSNNSIENQNIESSLINSNNSNSNNSNINSLDNSSVENKTNILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3F0</accession>
    <name>A0A8J4Q3F0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HTTM-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_001352</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000031">
        <property type="protein sequence ID" value="KAF2077349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3F0"/>
    <dbReference type="OrthoDB" id="15875at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011020">
        <property type="entry name" value="HTTM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052964">
        <property type="entry name" value="Sporulation_signal_mat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39535:SF3">
        <property type="entry name" value="HTTM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39535">
        <property type="entry name" value="SPORULATION-DELAYING PROTEIN SDPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00752">
        <property type="entry name" value="HTTM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="323"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="351"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="593"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="HTTM-like" evidence="6">
        <location>
            <begin position="33"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2077349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="774" mass="89094" checksum="3A606C746707B395" modified="2022-05-25" version="1">MGKFKIKKPQDLSLKSIFSRDTMQSFSNKVTAMFGMDRRSIAAFRVVMALCVIGDVIERSTDLRVMYTEEGVMPRSLIVSKYSSNFFMPVHLINTSFGFQAFLFAMHIVFAFCMLIGYRTKTFSFLTWFMTISLQAYNGIVGHGGDVFFRMMLFINMFLPTTEFFNIDNASFYDANYIKSKNDYRRINGSGAVGNDESQQQILVDQNQQQQSNNNIVDVSSDPMDKKDILVGDTNIEMGENNSSVSLSSSSSSSAKSHPNQYRYLSFATFCVLLQMGLMYSTSYFHKSGEEWKNGEATFYALTLDYFATDFAKFIINFRTPLRLLTIAVAKWELAGIFFMFSPIFTDHCRLFGAIGFAFMHLGFVLCLRLGLFFWVTAGAQIINVPPFLWEMIYGYCEKKILKGQRPVRIFYNTTSPMSQKIALAYKTFFMLSSTASFAPINHLKDEITISTMSPTSATPFESESDGEYDDADEVQRSVHKRHAAGISSNNNYTLSGGSSSMFGKKDILGDDWLVVIDSNGIRKRNVTALNYITSKSPLLFPFAMAFANVPQKVVDYFAKLMDSLHSRSQQNQILTGKRSPYQKKKIPRQYSVYYSIVNNIWMAFICWFLLAYNLNVFHINVGYKHSYHQLAYLLRLDQGWNMFSPQPPKSHWWHVIHGQLDDGTQVELFKNEGLFNFEINTEVNFEKPDPFYPSYGNHRWFKYWENGFNSNNADALRLELGRYICREFNSRHYGPEMLYKFSVYFVHEYQNLDGTVSASSHQSLWNHICYEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4L6</accession>
    <name>A0A8J4Q4L6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_004837</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000179">
        <property type="protein sequence ID" value="KAF2073841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4L6"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="G" evidence="4">
        <location>
            <begin position="181"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="377"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="98"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2073841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="507" mass="56943" checksum="4A9442E2526F1C50" modified="2022-05-25" version="1">MNSKGESNSSGTQIDALVFGENQQEYIIRENTDHEPDTEAENEDVVLSDGTVNGGSGNDQDSKSQNTDHKPDTKDNQAENGDVAHSVNGGSSNYQHKSKSENGKGDEKDNNEPKSEKDKTDVIVKKGNTGSQSANNRGTDENIFLKIGAGILLVALILSFLFNFIPYQEPDPISNQDPIAVLLAGNPGSTKSTICSTLADSPGKCRSGIAQNDVGGLTKEIQIVPMDGYVIIDTPGLYDPISLKNNCQEILKALTYSTKKKLIFAVLSTDGRIDGSESGMVNLILKSLPYKVPYGIIYSKVGRRSQETLKADQFHTFLEWAPFHYIILDKLEELVDKDDMISSNKEYNEKVKTFVDKTPYFNATKDKIKPIECFDIQKEIDQRNAQYKAEIEKERRLIQEQLEKNENLKSCKIEFKVEEQPFEETSVDQQQPHTRIYKWKPVKVIKTTTEIGILKTTYVRMVCETKSGDVITNQDWQETGKSRIVTFKDHNQTRRDVEVKDLLKMSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q571</accession>
    <name>A0A8J4Q571_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000254</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000004">
        <property type="protein sequence ID" value="KAF2078504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q571"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="513" mass="58290" checksum="E2F3E7410FA22072" modified="2022-05-25" version="1">MGESFYLIWRNQYIRRMIRNIVCQGVVINVDKEYMCDNQQYLSLFTNKEKLDYNISIRFKGDTIDYLDINNNRDMINVVDLKIKADFNFNEIHDGVHTLLLKLANGKRTAGMGQLPDSITKLTFFAMPPRTGAPPIVQHLISNLPCKLQELSFTYIDDCIHSRCVLPQSLTDLHYAGLCDSMKWLVVPPNKLYKRCDLELDSYESFQWLLENKFIGKIRIGPGAIPVLKSHPLPSHVTDVNLEYGVPEPDLLLPHTVERLYCSNSGTPFSHITQLKLLNIYDEYSIKLEKGVLPRSLESLFIRNNQPLEADVLPPTLTSLYMYRFNQPLCINVLPLSLTDLYLGAFNQPLNAFVLPSKLKKLNLRGFEQPTFFANSLPVSLTRLYLDAFKGSFDQCQPLDNLKRLRIDSLVPSVAGIVTNVKKLDLEVDTEISDPSGTCLANTSIESLYLSVKLKSTLYPNSLPPTIKYLSLVNVVIESDNVIPSSCRYLKSRNSKIDPISIPKSARYLKSIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q7A8</accession>
    <name>A0A8J4Q7A8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">WD40 repeat-containing protein SMU1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_002999</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the WD repeat SMU1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000089">
        <property type="protein sequence ID" value="KAF2075686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q7A8"/>
    <dbReference type="OrthoDB" id="538223at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045184">
        <property type="entry name" value="SMU1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054532">
        <property type="entry name" value="TPL_SMU1_LisH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17814">
        <property type="entry name" value="LisH_TPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CTLH" evidence="10">
        <location>
            <begin position="50"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="235"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="273"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="316"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="358"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="491"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2075686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="524" mass="59736" checksum="F11B39BE38D6920B" modified="2022-05-25" version="1">MQNNNNNVQPSNIEIQSQDVIKLILQFLKENSLGNTLSTLQEETGLYMNIIDNQESFLNDIRNGNWDKVLTVLQNLKLSLPTMITLYEQIIVELLHINELELVKHLLRKTEPMNQLKQTQIERYMKLENYLANKSINLVDFYPYGITTEKRRKHISELVREELTKIPSSRLLGLLSDALQWNRHIGKIPNDCTEFDLLSGKVPEKKVITEDDEPIKTLHKTIKFNDNNKPEVCKFSPDSKYLVTGSADGFIEAWDVETAKLSKNLPYQAADEFMMHDDAILCLAFSDDGEYLASGSLDGQIKIWQFKTGKLLRKFEPAHLQGVTCLQFSKNSTQILSGSYDSSLKIFGLKSGKALKMFKGHKSFVNGCYFTPDEDRVLSCASDGKIKIWDAKTAECIQTLSPIPTVSIKTITIKSLSFLNRHSELTIVCTAPSISIYNLKTQNIVKEFKSDNNQLFICCTTSTQQNYLYAVTEDHILYCFDIEKGTTLEKFKISDKDIYGLAFHPNYNLLASYGLDPILKIWKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q7B7</accession>
    <name>A0A8J4Q7B7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002959</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000088">
        <property type="protein sequence ID" value="KAF2075716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q7B7"/>
    <dbReference type="OrthoDB" id="9984961at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="206"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="41"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="374" mass="43093" checksum="EBAD4692C9E0A6F1" modified="2022-05-25" version="1">MIANQKLEDFERDNSRLQLENWEYKRVGCANTDHISLFTQLETSNNENDLLKQERNTLNHNVNLINENVEKLLLENKSNNNKIDLFLNKIESLVKVNDSLNQKIENLYEENKKSIDNYNNEIGLLSDKIKSLERVNQSIHQQQCTLKEQQHKSMEKIESLTKANESLHQQLKNQIYSLESKLSEQNLEIDDKESKTCKDIETCEQDLLFLKRHSFFKIIKFWINDKTIDFELLYKASNADFSTSSFHSTCDGKGPTITLIETTDGDVFGGYNSQSWSSDCKWYGDDKCFIFTLVNKHGIKPTKYSPGIQNTHYVGSGRNYGPIFGSGYDIGVGLEGGSRQRFPWTYCDTTGKGESTLTPTKYFAIKTVEIYKCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q808</accession>
    <name>A0A8J4Q808_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">GPI transamidase subunit PIG-U family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_002318</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGU family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000063">
        <property type="protein sequence ID" value="KAF2076361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q808"/>
    <dbReference type="OrthoDB" id="549017at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009600">
        <property type="entry name" value="PIG-U"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13121">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13121:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN ANCHOR BIOSYNTHESIS CLASS U PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06728">
        <property type="entry name" value="PIG-U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="167"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="197"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="264"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="329"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="393"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="426"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2076361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="472" mass="54508" checksum="DA043F981037E652" modified="2022-05-25" version="1">MNSTQKKQTSSSTTTTATATNKCDSTFKTFIFISLSALLLRFVLFYQGFHFHFSNRNEITTPLTNFKRLTEGLHLWDLGLSPYSGSAYHQSPLVLLLFYPFHSNNLSTSLTGLPPQVVFLVVDILIAYLLREITKLIPQVLPKEMKPLEPNSNLPNITAALYLFNPFSIFTCIGMSTIVINNAAIILSLYYSLKGNLFQSVFFVALSSYFSIYPIILLFPVSFILKRHYFPEQTLNTATRQQLSPQQQSHLEKQLQHSQTTMSILFYSRIVLFYLLSFSSLIYLSYTLLGSWEFLSKTYGFTLFVEDLTPNIGLFWYYFIEVFDHFRNFFLFIFQYHVFIYCIPLAIRLKNHPLFYFWALCAIIATFKSYPALGDTALHVSLLPLLNEPLKGVRYSFIVIVVAIYVTVLAPILWQMWIYQGTGNANFYYTINLVFTLAQVLLIVDSLSVLLKLDYVNKINHLEENQEKKKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q9K4</accession>
    <name>A0A8J4Q9K4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitochondrial substrate carrier family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_001260</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000029">
        <property type="protein sequence ID" value="KAF2077411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q9K4"/>
    <dbReference type="OrthoDB" id="428293at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006862">
        <property type="term" value="P:nucleotide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044712">
        <property type="entry name" value="SLC25A32-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45683">
        <property type="entry name" value="MITOCHONDRIAL NICOTINAMIDE ADENINE DINUCLEOTIDE TRANSPORTER 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="94"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="206"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="304"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2077411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="392" mass="43525" checksum="BE8CE10F8E58C3AB" modified="2022-05-25" version="1">MKIKKSSSFLLISKNRNNKGSHSNSNPTYNRFGLFISLSKTTTFFFITFLFIIWMDKAHAREEYEFAATKQRFEDHIPTIISDDIISHHKPKEFNLGIEMLAGTMAGVSTCILFYPLELIEAKIQVASSTTALNKGGSGVIKRSPIGPFSIAKSIFKTSGIKGFYQGLTPTALGNSVNWGVYFSVYRYTNHWWNHSSGGTSSANQPAWVGHSFSAITAGLITTAIVNPFWVLKIRLATSRKYNGMSDAFQSIIRNEGVKGLWKGVGISFFGVSEGLVQFVTYEYLLEQMKLMNGGSTSHLSMSNYLIAGGTSRLVAGLCTYPYLLIRSSLQSETCPYKSMSDACRQIYKHQGLSGFYKGIAPNLARSIPPAAFMLYIVEFFRNTLTNTFIAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4QBW9</accession>
    <name>A0A8J4QBW9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000057</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000001">
        <property type="protein sequence ID" value="KAF2078686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4QBW9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="101"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="209"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="452" mass="52881" checksum="B17D0151A0E343C3" modified="2022-05-25" version="1">MSNFFQFLSPIKNFQDFTLPPDSENDSPEENHFSRLSKLYSTSINDIHPLDLDFSHIPSPLQTAINTTHNFDPRLNEISPLPHHTCLEQNSSANVKNGKLVQKLQNDLVSAKDEISTLEQKYHNIKAENKKYKESNQKTEKLKKEIMYLKKNLHELNQRNANFIDQINYDQGVSRRLKLENDNLHTQVYQLNNENLKLKQVVSQQISLLNQKNQIIDNLSNQIKKEKDESAEIVCNMGILNKYKDIQLNNNLPSSLDLNLELEKFINSIEEYYTNTKLMELNDHDLVPLISVIFKICFSKCTDFFKFPHDILLKYKKPSDKQTYYLKCLLYSLNDSHLNAAESCIEDVKNLILKFIDSFTELELTKKFVNDCCKFIWTVLLTHPEAAFDEYKEDQNEEIDFSFDKHKSFGKIENGKQMVVHTTIIPSILRVDNGAMETKSYVYLREKFRPIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPC4</accession>
    <name>A0A8J4UPC4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_009652</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000767">
        <property type="protein sequence ID" value="KAF2069031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UPC4"/>
    <dbReference type="OrthoDB" id="655030at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47178:SF5">
        <property type="entry name" value="FAD-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47178">
        <property type="entry name" value="MONOOXYGENASE, FAD-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="5">
        <location>
            <begin position="169"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2069031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="409" mass="45707" checksum="7673B9FFE7267E61" modified="2022-05-25" version="1">MSPTDNQNNNKILIIGGGIGGLVVAQGLKKLNIPFILFERDESSSFRQQGYRLRINPEGSQALKQVLSDDIWELFTSSCAVFNSGQTQIDAITGDISSRSSANRIHFPERDNMVFSADRTLLRCVLESGLKQHLEYGKKFERYEMIKSADGQDLIEAHFSDGSKYIGTLLIGADGLNSKVRKQFIPELKYLDTCGRAIYGKTPITNSLLTKLPASVMEWTTIISDSRPLTLFMEPIKFRPPTPGKETQLPIVQDYIYWVLLSQAQVFGKSDKELYAMSESELKQHQLEMIKDWNEPIKNIIIHSDESKSSYLSITSSKPDVYQWETNKNITLLGDSCHAMTPTGGAGANTAIIDAFHLVEAIKNGAAVNDLNQYECKMRQYAKVSIEGSLGGAKRMFGFKSIDDCKFID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPF8</accession>
    <name>A0A8J4UPF8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_009496</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat G protein beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000724">
        <property type="protein sequence ID" value="KAF2069181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UPF8"/>
    <dbReference type="OrthoDB" id="10255630at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000020">
        <property type="entry name" value="Guanine nucleotide-binding protein beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016346">
        <property type="entry name" value="G-protein_beta_1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001632">
        <property type="entry name" value="WD40_G-protein_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19850">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN BETA G PROTEIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25391">
        <property type="entry name" value="WD40_Gbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002394">
        <property type="entry name" value="GN-bd_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00319">
        <property type="entry name" value="GPROTEINB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="58"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="146"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="187"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="229"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="280"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="315"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="2"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2069181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="347" mass="38573" checksum="E2D24FE083D38D3C" modified="2022-05-25" version="1">MSSDISEKIQQARRDAEAMKEQIRANRDVMNDTTLKTYTRDLPNLPKLDGKIKVRRNLKGHLAKIYAMHWAEDNVHLVSASQDGKLLVWDGLTTNKVHAIPLRSSWVMTCAYSPTANFVACGGLDNICSIYNLRSREVPIRVCRELNSHTGYLSCCRFLNDRQIVTSSGDMTCILWDIENGVKITEFSDHNGDVMSVSISPDKNYFISGACDATAKLWDLRSGKCVQTFIGHEADINAVQYFPNGLSFGTGSDDASCRLFDIRADRELMQYTHDNILCGITSVGFSSSGRFLFAGYDDFTCNVWDTLKGERVLSLTGHGNRVSCLGVSSDGMALCTGSWDSLLKIWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPS8</accession>
    <name>A0A8J4UPS8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_009022</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000619">
        <property type="protein sequence ID" value="KAF2069661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UPS8"/>
    <dbReference type="OrthoDB" id="20768at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="5">
        <location>
            <begin position="214"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="74"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="223"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="97"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="223"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="241"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="255"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="281"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="331"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="341"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2069661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="44393" checksum="1513EEC1F43144E9" modified="2022-05-25" version="1">MKIFIKGISERTSKSDIEDYFKKIGPVIGVQTGTNDFAFVDMQYESDAIEALGLEEIIIDGHKLKIERAKTSDEGRLSYGGGNNNNNNRNGNGNGNGRDHRDNRDNRDRDRGDRYDDRNRDRDHHRDHRDRYDDRDRRDRYDDRDRGDRYSSRDRDRYDDRYNNRDRRDRYDDRDRRDRYDDRDRRDRYDDRRGGGGRDMRGGGAGGMANGQTCFNCKKPGHFARDCQEKPRNPAYADTRGTLKDERRDRYHPYGGNGGGSGGSPRRGYSPRRRDDDDDRRDDNRRDDRRDDRRDDNRRDYDDNESKGGDSPIRQRERDDKSPDYSSNNNRENRDNRDNNGHNNNSSNNSPPSPGNNRNDRDNRDSGNNNNQDDRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQ90</accession>
    <name>A0A8J4UQ90_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008322</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000502">
        <property type="protein sequence ID" value="KAF2070366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQ90"/>
    <dbReference type="OrthoDB" id="21056at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="106"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="226"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="276" mass="31221" checksum="5C64CA89060BDBD1" modified="2022-05-25" version="1">MSRFKERNNFYIRCAKQYVVPLLLILHDYKQFTEIQFQELVLVLQGILQQHLTSLKGNYHSTILTVEQSSSSKPCSLSDFSFTFKFTPTYPKYSIFINKLRNSSTKATTTATNTNPTSKKRKNDIDDDTFEEIKTKFDNDDHEAPIDVDINDPIDVDDQKKDNKKQKEQEKEDEEEEDYKGGVYDGLTVTGHTLVIETFPKANNSIAPNDLFAEEFVSPNKKSKKTTTTTTTTTSSSSTSSTTSTSPSTGPKTNRKSKSSISETNSNNLISTYFCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UR42</accession>
    <name>A0A8J4UR42_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_007090</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000360">
        <property type="protein sequence ID" value="KAF2071581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UR42"/>
    <dbReference type="OrthoDB" id="7851174at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000002">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="5">
        <location>
            <begin position="5"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="3">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2071581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="151" mass="16762" checksum="612255911A9E93E9" modified="2022-05-25" version="1">MANTTSTKRIQAELANIITEPPPNCSAGPVGDNLYEWMGTIMGPEGSPYEGGVFFLNISFPLEYPFKPPKVTFVTKIYHCNINANGEICLDILKTMWSPALTVSRVLLSISSLLTDPNPKDPLMFAIAESLLNNRAEHDKTAKEWTERFAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4URT5</accession>
    <name>A0A8J4URT5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Leucine-rich repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005986</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000256">
        <property type="protein sequence ID" value="KAF2072706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4URT5"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052394">
        <property type="entry name" value="LRR-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="114"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="225"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="793" mass="89592" checksum="DC4818402BB45006" modified="2022-05-25" version="1">MKDKDKELEKIYKKEKEKEKEKKKSDSIQIESKSRSISSPPNIIVDKIDNSNSNSNNNNNSINNNISTLSNSFDNNLNVQPKERRPSSPLAFLSSLSPSTRKKKKKEGFKDLNDLSLNTLSTSPPKNDHHHHHHHHQHRKSSIDSDDQQQNENYLNRPWLEPTKFIKGESININQLPHTVLLKILHYLIQHKIDTEKSEIRFEQRSPTEEGGQNLNLSGQGIETSSPLSSSLSSTNHLGGKKISPNYELESISLVCKLWGMELAPQIFKFFIVKSPKHLKALIRLTSQGLLEGGRRFEFLYIAMILDKSSTFQKFMNIVKHTMPDKVPEKFLKATTKPILSNTFSKHLFTSFFDTCKTMKYFRFYQKWVSPENFTAIGNALRTNHSVTHISFRHNNLEDPFVEDIINALYENNTIEALDFRGNKLGNQSAIKLAGALLRNHTLKIIDLFYNNIESEGGVALCNALKINKSLKKLYLRWNHIDSQTAVVLSDALKQNTTLDSFHLDRILDHGGAALFDAIRQNHSVTEINLSDCSLKDGSAIAISKAFIENSTLKDINFKSNLLGSSINLISKALVSNKVLTRLNLSDNRIGDSLGRDLGEAFLTNESIKSLSLSLNHLGNLFSERMAKSLLINNTLEMLDISGNQIDFEGAKHWAETIATNKTLKLLNLSQNSLSPKFGIVIADALKSNKTLIHLELAYSNLGDEGAIQIANVLASNSTHIRRLNMSENHIKDSGGIAFAQAISTNEFLTDLDLSYNNFNYKTREIFDKNTKSNSYILNISYSQVPLRWKFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4US99</accession>
    <name>A0A8J4US99_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005311</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000208">
        <property type="protein sequence ID" value="KAF2073366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4US99"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="201" mass="23048" checksum="D6CDCA61533A469B" modified="2022-05-25" version="1">MQNSKFKINTWETKTKSDDQTFKVPLPVNKSKRINNYNNNKKTCTTNKMIRVSKPITPFYFPRVMVQEFDLSKFKPQQQENKCKESVDIQLDITELKRLSETCLEILANSNNNFRPISPCAVVPSCTLVLPTPIEQSSSRVPSFSMDNIQPSIEIENPNSNVPSQLSTSQYCNIYLPSFSDYNSQRVPSSDLELPPLRDLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UV37</accession>
    <name>A0A8J4UV37_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002457</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000068">
        <property type="protein sequence ID" value="KAF2076216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UV37"/>
    <dbReference type="OrthoDB" id="783096at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07383">
        <property type="entry name" value="MPP_Dcr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000172">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011230">
        <property type="entry name" value="PAP14/16/28/29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32440:SF3">
        <property type="entry name" value="CALCINEURIN-LIKE PHOSPHOESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32440">
        <property type="entry name" value="PHOSPHATASE DCR2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF030250">
        <property type="entry name" value="Ptase_At2g46880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035185888" description="Calcineurin-like phosphoesterase domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="2">
        <location>
            <begin position="49"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="388" mass="43712" checksum="3A6B344967648B6B" modified="2022-05-25" version="1">MTSLYLLGSLFLLFILCPNYEAINVSKNNIRVANENVDKDLVFNSNGQFKIVQFTDLHYGETVYYDAFSANAQNQVLIAEPDADLVVLTGDCVSGYAWNGSAGWLASKWDHMVSPMLANNKRWAMTLGNHDDEADLDRQQIVEYDSSFNLSLTQQGPNDLHGATNYWLPINDQNGEPQTILYFFDSNDDNCQNITGWGCVYPDQIEWYRGVSLSLQAKYNRIIPAIAFMHIPIPEYMDMWNFYPVNGSLYDTGVCCFSVNTGLYAAFKEMGDVVSMHVGHDHDNDFIGNYNGVQLGYGRKSGYGGYGPPPSWKHGARVLEITANPFSISTYLRYEDGSSETQAPYHLPQAKEQYNTCCDTYGFVNVTGNPISNCKLYEQNFKSIHSKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVH0</accession>
    <name>A0A8J4UVH0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002127</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000056">
        <property type="protein sequence ID" value="KAF2076576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVH0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="192" mass="22445" checksum="03A9E3208D658A08" modified="2022-05-25" version="1">MSDVYVDQILNYVQLSEDVDPDTLQLLYNTYPHARLPIYCLVQPILKGDKELLEVFFRNQNFADMVKKVNFPFLVNKILKQRDPALFNMVMEHFNPPLYEFIVQNAPTVQPYLFFLSLKQNQFSYPQQCFGNMGLGQSFDMNSLGNYSKVITGVEVHMGIEKEDIRLLIEDRGSFILILKDWNIDKINAMEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UW83</accession>
    <name>A0A8J4UW83_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pulmonary surfactant-associated protein B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Pulmonary surfactant-associated proteolipid SPL(Phe)</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_009247</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000663">
        <property type="protein sequence ID" value="KAF2069435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UW83"/>
    <dbReference type="OrthoDB" id="19956at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.225.10:FF:000008">
        <property type="entry name" value="Pulmonary surfactant-associated protein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.225.10">
        <property type="entry name" value="Saposin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007856">
        <property type="entry name" value="SapB_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008138">
        <property type="entry name" value="SapB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008373">
        <property type="entry name" value="Saposin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011001">
        <property type="entry name" value="Saposin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008139">
        <property type="entry name" value="SaposinB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051428">
        <property type="entry name" value="Sphingo_Act-Surfact_Prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11480">
        <property type="entry name" value="SAPOSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05184">
        <property type="entry name" value="SapB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03489">
        <property type="entry name" value="SapB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01797">
        <property type="entry name" value="SAPOSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00741">
        <property type="entry name" value="SapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47862">
        <property type="entry name" value="Saposin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50015">
        <property type="entry name" value="SAP_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Saposin B-type" evidence="10">
        <location>
            <begin position="23"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2069435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="131" mass="14331" checksum="C2EC15A59C89EEDC" modified="2022-05-25" version="1">MLSILGGCEAKGFGVDVDVAVGTVEECKLCTYFVGEIEGYLENNSTQEEIITYLNNYCDDFFGGVASTCEALVQQYVPLLFTMLANDEDSSVVCKQVHLCDGSSSSSNNSNDETIEKIIKSFVKLAQQREN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWT0</accession>
    <name>A0A8J4UWT0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_000813</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRT-like transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000017">
        <property type="protein sequence ID" value="KAF2077851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWT0"/>
    <dbReference type="OrthoDB" id="416555at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013936">
        <property type="entry name" value="CRT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017258">
        <property type="entry name" value="Transprt_Chloroquine"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31326">
        <property type="entry name" value="PROTEIN CLT2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31326:SF1">
        <property type="entry name" value="PROTEIN CLT2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08627">
        <property type="entry name" value="CRT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037671">
        <property type="entry name" value="Transprt_Chloroquine_res"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="84"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="144"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="238"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="306"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2077851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="454" mass="49929" checksum="0DE04E62FFFFA94E" modified="2022-05-25" version="1">MGQDEKKSLLANQDGGQDDVEISSGEGKKKSLRDYIPKLSKESITILIYVVLYITCGVINSVLLKQVMNTFSNYGFFLNQLTNFGYIPIFGVAVAYKMFFTNDIPKETRQFPLYKFLIMGALDAVTGYFVVIGGISTSGPLQQLLNQAIIPITMLVSFFILKERYSWIQLVGAVVIVGGVVTSLIPSLTGGGNSGNKPFWNFFYLISIIPYAFSNVYKDIGFKAVDDMDVWYLQYWDCVFQSIIGTILFPINAALPPPATIPFNSIGPSLKNGSLCLAGHNNILATDSTCGPLTTDPMPCDNCHNAWIIILVYMTINVIYNVFILLVIKYAGATVFSIANTLRLPLTNIVFSLKFIMGKQVVPFSGLSIAGLIIILGGLTTYRVGSTIKAKKAGATEQVRFIPGMGPAGVEGIVPIRKKFIEPKSQEHLRKQFFGKLGINIPEDRYNKAHINDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWZ2</accession>
    <name>A0A8J4UWZ2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000684</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000013">
        <property type="protein sequence ID" value="KAF2078046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWZ2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="72" mass="7540" checksum="7415B31942474EFD" modified="2022-05-25" version="1">MLIKNITKLGKGPSMSKSSSTVSGASGDINFGDNQTAFRSGSLANVDFVLNLSNFTIDFILQLLGLTLSGNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UX15</accession>
    <name>A0A8J4UX15_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">VPS9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008321</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000502">
        <property type="protein sequence ID" value="KAF2070365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UX15"/>
    <dbReference type="OrthoDB" id="300289at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.80">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045046">
        <property type="entry name" value="Vps9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037191">
        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101">
        <property type="entry name" value="RAB GDP/GTP EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101:SF52">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN 9 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00167">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109993">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VPS9" evidence="3">
        <location>
            <begin position="704"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="30"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="122"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="205"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="286"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="360"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="444"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="505"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="556"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="852"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="962"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1035"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1058"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="124"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="166"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="289"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="306"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="364"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="450"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="505"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="524"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="570"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="862"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="968"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="982"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1006"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1038"/>
            <end position="1058"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1156" mass="127814" checksum="0121E81C6567F315" modified="2022-05-25" version="1">MEPSIKVNSNVPLVDLDGCDIDKLSLLNSNNNSNSNSDSNNNDSSNSSTIDSNSNSNRIDQQQPLSKGSGFSRSKSFDSHDNIDFDSYYGGSDDDSSSSLNNKETNLKNNLNSIAENDLLVDLSDNSNNNNNNNSGDGDININNTNTNTSENSNNDDEQVKEQEQQDVNVEQQQQKQENEIDIKKSFSFEMNDLFLSPTLVNNSNNNNINNSNGSSNSTGSNSNNDNDNFENNIDVFEDSIETFLNGISKKKEKQKQQHQQHLDEALFNYEPKRLSEMKNSNSIEDFASNSSLSSSSLPPHYRPPTTTTTTTTTTTTTASNSSNSTTSSPGSSYSHHKSLSSSSYVATPTIAILPDFKSDDIKSPLSLSSSPSTSNKDIVNSIINTSSNSSNSSPNKESTSTTTSSSSTTTTTNNNNNNTNNNNNPKTGFFTRMFRRDSSKTLLKDEKVNGNSSNGNSNGNSSNPIPIANNNNNNNSNNGSNGSHNSTEENNFILNTISNLSQSLSQNLPNLPSKSSSAPSTPVNQGTKAHAKTNSTTNHSGAKFDNLLLDDDYEIDTPELNGSGGNNLSLPTSPSSSGAAGTLTSTSTPTSRGLTKKKSRDFNKLLEQNEIYSYEHFLEKLENASHIKASIDSFIRTLKNRKVANDDLREALLDFVDNMQNTLTSDLQWTHCSETELGYASLHLQQYVLEKIYDYVFRSTEDEDKDNVLSELINKLSFVTIDHLEIPHYDFNEEMWQKAGAYLLRINFTKSCRHKVMYITKTCKTILKYLSTFLGEVSGADTLLPNLIYVVLKTNPPFLHSNISFISKFSETNDSEAVYYMTQLVSVIYFIENITHSSLKIDKKEYYQRMGTTPSPVLPRNNSSSSLSSSSTTTTNSTTSPKSSNGSINITNNNNNSYNNAMDQSMANDNALATLVEMFPDCDTQYLLDCIKYIETKHNDISLNDLVIRVTNLIAEHQGNLYIPSPKNQSNNNNKNNKMNTSGSISISKDYSDNTSGGNSFSITDDGSGNNDDNNDSFNIIESIMNQSEPIIVHSRNNSSSSSNNKNNNNNNNNNNNSKYQLIIEQKEKEIARLLEKTRQDQIEILELKKNLQGKTQEINSISKTIKNIKVPNASFVNSKIDDFKVTEVPMLLSEYKQIYNGFKNTTDALKKFNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXG8</accession>
    <name>A0A8J4UXG8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL30m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_008209</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000487">
        <property type="protein sequence ID" value="KAF2070468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXG8"/>
    <dbReference type="OrthoDB" id="509901at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01658">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01371_B">
        <property type="entry name" value="Ribosomal_uL30_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005996">
        <property type="entry name" value="Ribosomal_uL30_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018038">
        <property type="entry name" value="Ribosomal_uL30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01308">
        <property type="entry name" value="rpmD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15892:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL30M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15892">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00634">
        <property type="entry name" value="RIBOSOMAL_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL30-like ferredoxin-like fold" evidence="6">
        <location>
            <begin position="54"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2070468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="113" mass="12877" checksum="BA40558C9E7ED207" modified="2022-05-25" version="1">MSTGKITSTMITQLKRKVFQDVPHNILTRFPDFVVKSLPEEFIKQLPKAPAGVIKVTLHKSPSHAIPEIKGTIKALGLEKRFHSIYHKNTPEVRGMIHKANRYVRVEEIPINN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXY2</accession>
    <name>A0A8J4UXY2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_007578</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Endoplasmic reticulum membrane</location>
            <topology evidence="2 10">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the OST1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000412">
        <property type="protein sequence ID" value="KAF2071113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXY2"/>
    <dbReference type="OrthoDB" id="310030at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007676">
        <property type="entry name" value="Ribophorin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21049:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21049">
        <property type="entry name" value="RIBOPHORIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04597">
        <property type="entry name" value="Ribophorin_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035341022" description="Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1" evidence="10">
        <location>
            <begin position="23"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="412"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2071113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="448" mass="49899" checksum="EE58CA3714CFAF02" modified="2022-05-25" version="1">MKGQVLFFSALALILLISGSMATVASSWVNQDVVRSIDLTSQLSKSTIQIKARALKESSVYQLAIDSSYHSSIQVFDEDSRLPLLVKFDSISHKKVKSEGVLNLKVIVTLMGQMKPYPEHITQTQGQLVKYTDNHYFSTPYKTESQKTTVKLATAKVESASELSPSSVKGNTVSYGPYKNIEAFTVSAMSIHFENNAPFLVITSLIKEYEVSHWGNLAVETHYDVQHKGAALKGAFSRLDFQRQPSPAHIAEIQEIVPKSAADFYYRDSIGNISTSSYTHHPNHLTLKIVPRFPLMGGWKNSFYAGFNLPVGQFLSVDSDTGSYVLNVTFGVGIDNIYVESHTVKFILPEGATDIKVTAPFDVKMSQENRKTYLDTVGRPVLVLHLDHTAAENYQYIQVSYNLSSMAMFHEPLLLIGAVFALCVLIMIYVRFELSISKNNIVVDKKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYD4</accession>
    <name>A0A8J4UYD4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_006130</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000269">
        <property type="protein sequence ID" value="KAF2072555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYD4"/>
    <dbReference type="OrthoDB" id="515666at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052435">
        <property type="entry name" value="YY1-Transcr_Regul"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16088:SF3">
        <property type="entry name" value="GON-4-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16088">
        <property type="entry name" value="YY1 ASSOCIATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21227">
        <property type="entry name" value="Myb_DNA-binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="861"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="102"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="229"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="309"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="408"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="811"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="102"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="118"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="234"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="331"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="408"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="442"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="811"/>
            <end position="846"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2072555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="915" mass="105503" checksum="F053FFCD3E0CA737" modified="2022-05-25" version="1">MMESNIYNITTSVANGGGGGGGGGDILENQQQQILYNQYNHFHQQQQQQHHHASLNNNIWFDDQMFPMEHSPTKCTENSTPISIFPQSPGCPLRFSASPTSPLHISFSPTNKQTSQRTSLNNSTNSNGSLTSTPITTTTTTTTTTTSTPTTTTTIDLDEIIKTNDGRISKRTRSHMPLDNIDIDLLDQYFDEKQLVPLDNTFESEINSLTTDINSSYYKNFLATLQSGTTNTIQDDEDDDEDEDYIPDEEDEEETFDLGDKNDPIVSESISNEMEALVSDFIENEVKDYDFCKLLDIDFNNQIVDNINSNSNQENNNNNNNNNNNNKNEQVDYSNRSEQQQQIEQTTLYPLEPVQDIDISIFQETLKNCNLNFVNTQDIYSSILNNTPLFNNIFIPIENSNIESTHQNLSSLNNNNSNSNNNNSSDDFSIHRSKKRVKFSQPSTSTPTTTPSLQITNINNIDTPTKSMKPIVYFSQEDLIELKKQISQHYQLLLQSYLLLRVQSNPDNFKTTKYKKRKIKSTEANINNNNNLQEIYVDSNDEYTKNLVFMDNETLMEKYKNVVRPTSNIPLMESVNNMLERVKFMLEDLFTQHQKYQEKQVLSRIIFHQLESPSKVLTRSAKKALAPHIPSFFDCELFKLHPYLKQMVQEDAKPTLQTMEIILSSFSDYLSCELTYTNCKSNQLKFSPGEDLLLELGIKKYGIHRWSDIVVDFLPSKTVDQVFHRYKNRVSNSSADNLIKDYYHTQKFTKEDEEILMQGVKTFDMDWDKISANLLPKKEALTLKKFYEKNQQITQLDSPINNNNIININVHSNNNPINNNNNNNNNNNNNNNNNNNNNNNNNNNNNTRFDNIEYVNGVLIKWTREEDRTILLTIREKSVCKEVWNELSQKIEEKTSEQIENRYKHLLSLMQKNKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYL3</accession>
    <name>A0A8J4UYL3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA-directed RNA polymerase III subunit RPC3</fullName>
            <shortName evidence="5">RNA polymerase III subunit C3</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_005620</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the eukaryotic RPC3/POLR3C RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000229">
        <property type="protein sequence ID" value="KAF2073065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYL3"/>
    <dbReference type="OrthoDB" id="272392at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000420">
        <property type="entry name" value="RNA polymerase III subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055207">
        <property type="entry name" value="POLR3C_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013197">
        <property type="entry name" value="RNA_pol_III_RPC82-rel_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039748">
        <property type="entry name" value="RPC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12949:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12949">
        <property type="entry name" value="RNA POLYMERASE III DNA DIRECTED -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08221">
        <property type="entry name" value="HTH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22536">
        <property type="entry name" value="WHD_POLR3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase III subunit RPC82-related helix-turn-helix" evidence="7">
        <location>
            <begin position="7"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase III subunit RPC3 winged-helix" evidence="8">
        <location>
            <begin position="487"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="241"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="243"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="269"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2073065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="644" mass="73286" checksum="73228634128B1FF2" modified="2022-05-25" version="1">MYEQKVAIDVVKESFGDDVAKVYEFLVLRGKSSLKIIVQSVEGLKLKRIKECLIILIQHNLVSYEEFLIQLTKDERAKLEPNDPLPTDSIYEANLKNAIHRIRFTKFIHFIKASRGDDYSIVIEELIENGRLTMDQIINQSCAFYNSQYPERFENDLSKKFEEIFTHLIMDQFIMRAPKPKPQSDTEVTTALNKEVKNNSKNNQKQKEIDPFSLPAAFSSTGPLTDVNIIPNPAISISVETASRGSTPGSSSPTTPSKPGNHAKKTSAKKAAALLAEKNAAAAAATTTTTTTGKKRKNQESTTPEDSFPDVLMLAEGGSVDINNSEFQPTQKKRNGLEYTISSHEDDQQKLINDEKKVLWMVNYEQFITEFKLKACYDYVVEKNNVQSGQLFMAMIKLCKKSIRSNSDTLTSCVYGENILGQYNEHLEENTRMDKLQFEKYLTLMQASRPSIVTKMQTKSKQSTSTDLGAYQVNIGNIIGVVKQKLVESIIKQRFGDNGLRVFKLLLIKNLLEPKQIADMAMIPIKECKTLLFNMMQKSIIRLQEVPRSSDHFANRTFYLFFVDMNTIIRNSIDDIFKSIHNTRQRLIYELAPHKDILSKMESLDEETMNPDQSKIVKKVTRVNEILTTVILNLDNDLLQLYSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZS1</accession>
    <name>A0A8J4UZS1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BP74 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010438</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001112">
        <property type="protein sequence ID" value="KAF2068237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZS1"/>
    <dbReference type="OrthoDB" id="2232403at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056422">
        <property type="entry name" value="BP74_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053344">
        <property type="entry name" value="cAMP-inducible_BP74-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35883:SF1">
        <property type="entry name" value="CALMODULIN-BINDING PROTEIN CAM-BP15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35883">
        <property type="entry name" value="CYCLIC AMP-INDUCIBLE PROTEIN BP74-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23621">
        <property type="entry name" value="BP74_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035287371" description="BP74 N-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="BP74 N-terminal" evidence="2">
        <location>
            <begin position="41"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="106" mass="12142" checksum="61A185BF682683B0" modified="2022-05-25" version="1">MNKLLIGTLIVFATFLAIASCDEAYFGFKTHANEHEFEESKSQKDYNPRYSFHLDPENISFFQMAIEVCDASLQYTEDHLDEACGAFLPGCVYCPWSSKLTREIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V095</accession>
    <name>A0A8J4V095_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AP-2 complex subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_009737</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <location evidence="1">Coated pit</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000792">
        <property type="protein sequence ID" value="KAF2068942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V095"/>
    <dbReference type="OrthoDB" id="28053at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030122">
        <property type="term" value="C:AP-2 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000020">
        <property type="entry name" value="AP-2 complex subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050840">
        <property type="entry name" value="Adaptor_Complx_Large_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017104">
        <property type="entry name" value="AP2_complex_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003164">
        <property type="entry name" value="Clathrin_a-adaptin_app_sub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008152">
        <property type="entry name" value="Clathrin_a/b/g-adaptin_app_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009028">
        <property type="entry name" value="Coatomer/calthrin_app_sub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22780">
        <property type="entry name" value="ADAPTIN, ALPHA/GAMMA/EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02296">
        <property type="entry name" value="Alpha_adaptin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02883">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037091">
        <property type="entry name" value="AP2_complex_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00809">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55711">
        <property type="entry name" value="Subdomain of clathrin and coatomer appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0168">Coated pit</keyword>
    <keyword evidence="3 7" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin adaptor alpha/beta/gamma-adaptin appendage Ig-like subdomain" evidence="10">
        <location>
            <begin position="743"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="604"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="609"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="622"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="639"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="712"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="14"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="60"/>
            <end position="64"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR037091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR037091-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2068942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="975" mass="109004" checksum="D07860DADD6DC47F" modified="2022-05-25" version="1">MSTPNPNIAKTSMRGLTNFISDLRNAPSKENEEKRVTKEMAHIRKEFKENKNIDGYARRKYVCKLIYMYMLGYELDFGHMEAVTLLSSTKFSEKQIGYISIAILLHEQHEMLPLIINSFKEDLLARNDYFQSLALAAICNIGGKEVAEFLSPLIQKLLIANTSSPMVKKRAALAILRMNRKNIGFVTPDQWVERLVSVLDDSDFGVLNSLMSLLIELASENPIGWESAIPKVIQLLKKIAINKEFPKEYTYYHIACPWLQVKLLKFLRYFPPPEDSQGGQVLNEILNAIFSQSESAKAGTVNHKNSLNAVLFEAINLIIHLDSDPTLLKQTSSLLGRFITVKETNIRYLGLATMSHFANLSNETSIMIKKYQDTVLLSLKDADISIRRRALDLLYGMCDKNTCKHIVAELLSYLQTADYAIREELVIKIANLAEKFASNYSWYVDVILQLITTAGDFVSDDIWFRVVKIVTNHEDIQAYAASTVFTALQSRNCHETLIKVGGYILGEFGHLIADSPQSSPLIQFNILHSKYGTCSPSTKALLLSTYAKFVNLFPELTQPTQEVFKQHSNYIDAEIQQRACEYLNLTSLNEDLMQTVLDVIPAFSEKDNSNNNNINSSNGNSQDPRVSSGSGYHTPPPQQQYQQPVQQQQSNNNLQDLLDPFGLGLGGSPQPAQQQVAQYQQPQQQIVMQPQQIQQQQSPQEVQSPFGSGTHNFSPSSPNGPSSPINSGGAIDPLQLKILISFKRLCLASEGVLYEDNMLQVGLKSEYQGSQGRLMLYYGNSSAFPLTNFNVTFNSVSGVQLQPQSIAPVIQPKAQIQQPVTFSCTSEFTETPTVVINFLTPGKPITIHLKLPIIMSKFFDPLKLNSGDFFAKWKTISGKPLEIQEVFKPTKPVDINQFTKVLSDGLNITVLKGVDPNPNNIVAACLYPFGSNGQPIQCYIRIESNPQANMCRVTIRTQSAVLTNTLKSLLIAHLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0L1</accession>
    <name>A0A8J4V0L1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">arginine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Arginyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_002806</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Arg) + L-arginine + ATP = L-arginyl-tRNA(Arg) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000082">
        <property type="protein sequence ID" value="KAF2075870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0L1"/>
    <dbReference type="OrthoDB" id="68056at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07956">
        <property type="entry name" value="Anticodon_Ia_Arg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00671">
        <property type="entry name" value="ArgRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000006">
        <property type="entry name" value="Arginyl-tRNA synthetase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000058">
        <property type="entry name" value="Mitochondrial arginyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="entry name" value="Arginyl tRNA synthetase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00123">
        <property type="entry name" value="Arg_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005148">
        <property type="entry name" value="Arg-tRNA-synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956:SF11">
        <property type="entry name" value="ARGININE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="ARGINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03485">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM01016">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55190">
        <property type="entry name" value="Arginyl-tRNA synthetase (ArgRS), N-terminal 'additional' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arginyl tRNA synthetase N-terminal" evidence="12">
        <location>
            <begin position="2"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="DALR anticodon binding" evidence="11">
        <location>
            <begin position="449"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2075870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="564" mass="64011" checksum="1EF974CEBB9D13AA" modified="2022-05-25" version="1">MFQEEISKQLAGITEIDASKILECVESTKNKEIADFAVPVPKLNKFKKLVGKPDELAAQFASKIQLNDLIKDVSVSGTYLNFKINRMVQVQNILKEVIEKKEKYGFTNQGQGKKIIVEFSSPNIAKPFHAGHLRSTIIGNFMVQLFTNNGYQVVSMNYLGDWGKQYGLLAIGFEKHGNEQSLLEDPIKHLFEVYVKINEEAEADPTIHDKARAYFKKMEDGDEEALGVWKRFRDLSIEKYKGIYKRLNVSFDIYSGESLVSDGMIVEYDKLEKMGLIEDSEGAKVIDLSKQKLGKVLVKKSDGSTLYITRDIAAAFERKVNIGFDHMYYVVASQQDHHFRQLFAILEKMNYDWKKDCTHINYGLVKGMSTRKGTVVFLEDILNETKRKMLKTMQKNPEKFAEIENPEEVADIVGLSAVVIQDFNAKRNKDYDFNWKRMLTSEGDTGPYLQYAHARLSSIERKSGVEFNPNADLSLLVEPQAFDLAIAVGRFPEVIQTTLNNLEPSTLTTYLFELCRAFSSAYAELTVKGQEKNVAEARLALFVAAKQVLNTSLKILGLVPLERM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0U1</accession>
    <name>A0A8J4V0U1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002438</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000067">
        <property type="protein sequence ID" value="KAF2076260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0U1"/>
    <dbReference type="OrthoDB" id="2114940at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="12371" checksum="8DC64C8913C7676F" modified="2022-05-25" version="1">MNFLFGEKTCFVVHGYPSCPYYQKAQKLGQAIEKKNSRIQVDYIEVDREEWKDYIEKERMELKEHRAHYHYTCPLVVEGCDDEAKLFVGGYAEFLAQSRKRKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V129</accession>
    <name>A0A8J4V129_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000055">
        <property type="protein sequence ID" value="KAF2076620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V129"/>
    <dbReference type="OrthoDB" id="10518212at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="139" mass="16454" checksum="F05F3D68C2805F33" modified="2022-05-25" version="1">MGRYSIELLVYTTSEKIKIHQEMAKKKILKLILKSKITEPVLFNYIKQIQEDDSDDREWNTKLNEEFKQTSYTANKIIAIRKDLCLAITNELEIFKETMILQADNDKSSYLEMNINTSPIFAPLYCRLLQLEQFLFHLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V233</accession>
    <name>A0A8J4V233_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010186</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000962">
        <property type="protein sequence ID" value="KAF2068489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V233"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="174" mass="20788" checksum="681806BE3EB01340" modified="2022-05-25" version="1">MYSKNENILESKNVFLYHINQIYTLIKSLPIDNDVENFEILKDIFINLLNQIIVQVEEIYQPTDLNQHLFKEIYRLLPSLLVSIENRKTFIKLLKYSTQFLEYISHLNTVNSNSMNHFKSNVKILKHSYFDLYLGQKDNELVFITLFLKIFNIPTFSTSGYNVHFINFYFTLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V303</accession>
    <name>A0A8J4V303_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_006581</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000310">
        <property type="protein sequence ID" value="KAF2072107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V303"/>
    <dbReference type="OrthoDB" id="18465at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035249520" description="Ig-like domain-containing protein" evidence="3">
        <location>
            <begin position="19"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="728"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="775"/>
            <end position="806"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2072107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="839" mass="93893" checksum="370E515504AF8B0F" modified="2022-05-25" version="1">MIKFIIYLILISSILVSSQIVKEINLKTPLVGIVSSSDTPFAIGKFQSPTSQTVCPALSSFCYLTGSDKDEDAFVTSRSSASGNCVAMIGKKDTASILSIYDTDKNQYVDITSSKKLNFTNLVIDDTYIWVTYSNISGTYLQPFIHSKCSKIELVANKTFQIDESQPETIYSTLSIGTSRIIYIYDKEGAIISKYEVTPDLNNLIKDETQTLNIGDACYFTLFQGSYWLVSSPSGFRVYDSVNEKKVSDFYLNDASNTFCGPFISKGLEIYSIPSPGDSRLFKVVPALEVISNRPCFNQSNGEIELTGTMKGYTYQWLDNGKTDLKRKNLKEGIYKLKITMSATNISITQKIDLRAPADPMEFDLLPTCLGSSTGGLNFTSKRVPGLNYTYTVSDGTLNGTESTNNPLFNSLLGNKFFYVNVTTVDLNGHVCEYLSNATIPNGVIPEFDVEYLNVSCSNMSDGYIAIKNYDPKNYTYEIFPPVKISSQGILKNLQSMTYTIFMRLNKSKKKVYGCPNIVNIDIMEPREFKFPSYNITTPKCFGLNGSLSVEYIPWMEYLLEDQHGDIHFPYIVNINRTSKWRNLPTGNYSMTCTLKSLRDHGICKEVVKYIYIASPDQIQLNDTIVTSSDCENENLSNMLAYAHGGMPFNGTRYTFAGSYANGTNAHLNVSYWIDGVYNLTIADSFGCKVKYTNFTIKRPDGCILSHNPHEAEIVEAKAQEKKHSKRLGLILGLVLGLALPIVLAIIAVAVFLAKKYGFRKGRIPFHHRNIGAANSIPMKQNDSGTKKEKGTLSNDGDSDTDNQKRQQISYDEDLEGIVEEEACKCHQIMQVYHWNFYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3J0</accession>
    <name>A0A8J4V3J0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_006076</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000264">
        <property type="protein sequence ID" value="KAF2072617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3J0"/>
    <dbReference type="OrthoDB" id="361494at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031464">
        <property type="term" value="C:Cul4A-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000109">
        <property type="term" value="C:nucleotide-excision repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042238">
        <property type="entry name" value="Rad28/ERCC8/Ckn1/ATCSA-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46202">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46202:SF1">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="137"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="217"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="338"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="426"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="482"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="496"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2072617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="549" mass="61699" checksum="B0C6A310C778D90A" modified="2022-05-25" version="1">MNSYLYNRELGIINERLEILNYSQRRVRAINLSLEKEIISYHNGPVNSLDIDVAENRYLISAGADGLISIYDLFENLLMLNKEDNDMIKGGVIENPARPHQYNINQQSSRIEYQPIMKVSRVGGGVNNTGGTISKGISSIQWYPIDTGLFFTGAIDGTVEVWDTNEGQVSMKFDMDYCVNSISFSPTSTQHSLLAVATSDQKARLCDIRIRTSAQCLTGHRESIFSIKWSPTTSNLLATGSKDKTIRIWDIRKSDTFLFACDQYNDGTGNMMNNNNNSIGNQETTTTEPNDRAIPKRYSQKFHAPKKIVAGRGGQAINMFRNENQGGSPNASIKRDVPTAHNGTITSIVWTPDGNYLASTGNDNKIHLWDIGKAGCNTMIDYIGSHNPNKTPNQMSISSDGQYLFHPNGKTIHMYHLPTGNLVKKLLGHFEKVSCCTFYDYDQSLISGSNDKLLLYWDHSGEDKIEELEFIKQEIELQTHSNNNNFINNDQDDENNNNNNNNSYSSLNNNNNNNNNNNNNNNNNNNNNNNNNNNLTGVLPDEDRWSDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3R3</accession>
    <name>A0A8J4V3R3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NUDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_008621</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nudE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000549">
        <property type="protein sequence ID" value="KAF2070064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3R3"/>
    <dbReference type="OrthoDB" id="5877028at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051642">
        <property type="term" value="P:centrosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007100">
        <property type="term" value="P:mitotic centrosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047496">
        <property type="term" value="P:vesicle transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033494">
        <property type="entry name" value="NUDE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006964">
        <property type="entry name" value="NUDE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10921:SF1">
        <property type="entry name" value="NUCLEAR DISTRIBUTION PROTEIN NUDE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10921">
        <property type="entry name" value="NUCLEAR DISTRIBUTION PROTEIN NUDE HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04880">
        <property type="entry name" value="NUDE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NUDE" evidence="9">
        <location>
            <begin position="135"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="189"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="261"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="16"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="154"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="192"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="270"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2070064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="367" mass="41541" checksum="69B2CA1654576599" modified="2022-05-25" version="1">MATVEIPSFSTPQEEIQYWKKRLEEKQQEMDELETSFNEYQDFSKQLEEEMESELKAYEKKYNEIQSQHVRLKNDYEKTLDKLNEHSRESSRLITSLQDEVTKLQTLKQSLMVDKRKLEQDNDYLERRERASDASVHDLSDKLNKVMEENVWMQSELEESKQIADETIQRLRDDIRDLRNELTVRERKPSILNRSANSASSYTSEKSKRENPMSNNIPRPMKSQRQKAEDTASSSARAASLVVVSDLINLVSDLEAKIANYRKPNGGYGDNNSGGSNSSMNSINSFASSPTKLPSPTSPVLKHSNSFSNIKDIPLTVNFFDASNNHNSSSSSSSNNGGTGLFNNTSTGNNNIINSTTSIIDDANVPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V512</accession>
    <name>A0A8J4V512_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_004550</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDPK1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000163">
        <property type="protein sequence ID" value="KAF2074147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V512"/>
    <dbReference type="OrthoDB" id="347657at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05581">
        <property type="entry name" value="STKc_PDK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000191">
        <property type="entry name" value="3-phosphoinositide-dependent protein kinase 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000534">
        <property type="entry name" value="Serine/threonine-protein kinase PKH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033931">
        <property type="entry name" value="PDK1-typ_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039046">
        <property type="entry name" value="PDPK1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF232">
        <property type="entry name" value="3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14593">
        <property type="entry name" value="PH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="96"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="362"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="47"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="364"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="397"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="412"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2074147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="618" mass="69802" checksum="13A2ECEF1FA32772" modified="2022-05-25" version="1">MIDTGNDSNEAIDSGTNKDQEHQSSVDELNEMVTVTTITTTTTTTTKKNDEPKQQEQHKEEDNDDNSVKTQTQQNTNTTPSSATNTPTIKKNRSDFDFIKTIGKGSFGKVKLVIEKETGKGYAAKILNKKLIIKEKKAKYVNTEKTILDSLDHPNIVKLYYTFQDENDLYFILEYCPNGDLLDQIKDAGCFEIKVAQFYAAEIIVAIEYLHSKGIAHRDLKPENILLGKNMHLKISDFGSAKVLGNDPRTRSGSFCGTAEYVCPELLTEKSAGKQADIWSFGCIMYQLISGKLPFKGFNEYQTFQLIIKRELIFPQNFDPVAQDLINKLICLDPFERLSIADIKNHAFFEGIEWDQLSKQDPPVIIPIEPPSPCSSPNSTRKKRSMSAHTIGSPLLIQQQQEQQKQTIIDQKSQQNVCSPRSAVNNGNPATTTTTTSSNQNPIRPLLQRERSSTLQHTPTTVKPIMFDRTSSTSSTPPLSPGLQSPRVYLKPNNISTLDKEKRKQLKDQQMLELSVWNRFLLPTDEIILACVLTEKRTGLISKKRQLIITDTPRIFYVDPIKLIQKGEIGVDLSLSAQYKSNKHFIITSKGRSRHFYDNGCQSKQWVDMINDLKTLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V528</accession>
    <name>A0A8J4V528_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000022">
        <property type="protein sequence ID" value="KAF2077698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V528"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="545" mass="63358" checksum="18C9FADDFE6F1D8D" modified="2022-05-25" version="1">MRDTQLSPSVHLPLLLIQKILNIYIRQYILLIDKNSDQEKEEAKSKGKKQQPTSFCNFNTIYNILIHFERVSKFWNKKLLIKLEFPNIIVLSNLDEINRFARFSSIREYQFYSNHQFKVKVNSLNISSPSLQSIALALIASKYGINIKSNTSLSDMEFKIICSVFYSFNGIGFQYKDVFDALELSYRLFGTTNPIKKIIVGMFDDVDSISKIQPILDKYNHIEKLYIFHDSNLLRGSIKSQNIKKIKIQQAMDHTCFGNILKGCHNLEKLELIEPFPEIFQVESFIYHPCLTNVSVQRESFKLMDLANYLTRNTNLVKLSLLKSKQLGVFIQDTVDGNYIDIYNTSLKYLTLENFHQFGDFLLKSWASQSGIEYLYISSHILSYDSFRMHNGIESLHISSLKNELGLCMLTTFELLPWSKYEFGYPFSVSKIFQFPMSDTVNFLVLEYNLNDKSCLEHLKCLPENIKYIKINAQTIPFTSLKASIIKTIIESNPHLVYFGLGNKIKFDIPIQELAICILNSKLKYFKVYQDHLSNELNSFYKLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V532</accession>
    <name>A0A8J4V532_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004493</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000159">
        <property type="protein sequence ID" value="KAF2074207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V532"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="6"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="353" mass="41037" checksum="25511D68278AB962" modified="2022-05-25" version="1">MDDSLILHYIFKNLPNDVFCLSLVSKHWNQQFENNDKLWKDLTFSLHPSLSDLPHNYDLTNVYGKKIRSNRLCLDGKNQYLLGKKATTIWKELFQQLFIKDNLLKNHLYELNKATRKMGNYNQVIACFRKYRMLNAYQSLNRNEYLNKFKGNSQLLMILDSVLYKTISSTIKHSYDGCDDGMGRNDLISLQGILFSTSGKPIDFNFKHQCQIQGGHSDGYSMNSKFKLGRATFESQNSSYYPGEIDTDVLDFVKSCRDELTHNNNKHLKLSHVIDLIVSIFPKEFLENTALNHPFCSLKNCKGVYQLGGEFDDEQDEEYGDDEQDEEYGDEDEGEERKSKLTDKRSLKKTKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5C7</accession>
    <name>A0A8J4V5C7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">15-cis-phytoene synthase</fullName>
            <ecNumber evidence="2">2.5.1.32</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_004306</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 (2E,6E,10E)-geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:34475"/>
            <dbReference type="ChEBI" id="CHEBI:27787"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="EC" id="2.5.1.32"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.32" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000150">
        <property type="protein sequence ID" value="KAF2074372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5C7"/>
    <dbReference type="OrthoDB" id="270318at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016117">
        <property type="term" value="P:carotenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002060">
        <property type="entry name" value="Squ/phyt_synthse"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31480">
        <property type="entry name" value="BIFUNCTIONAL LYCOPENE CYCLASE/PHYTOENE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00494">
        <property type="entry name" value="SQS_PSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0125">Carotenoid biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2074372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="366" mass="41883" checksum="C5D20B13E1960F43" modified="2022-05-25" version="1">MINRLSLNVLGSCKKIGSNRLLVSSTSNIYNKNVLNFSTSTKTTNNNNKDKLTTKSIQDKVENITAKENVASPSTKSISIDEIATPQDYNYVFERAKLSDKENYRCSLLINDPSARRASYALRTFNIETVSTDFGVKTEKMAKLRLAFWKDAINNIYNGKVYDQPLTRVLAQIIKEKQLTKTWFIKLLNRREKDLQSVQLRDMDELEAYSDDIYSSLILLTLEAMGVKGNHDIEHCASHLGKAVGIMVLIRGTPFHVSRRKLYIPVELTTKYGIKPEQLFRGEVDVPALKNAIYDMASLAKLHLEKARSFKAPPPTNQAFLIAQMVEDFLRRIQQVDFNVFKYEEDTTPDALLLIKLYKNKFFDKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5M3</accession>
    <name>A0A8J4V5M3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">gluconokinase</fullName>
            <ecNumber evidence="3">2.7.1.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Gluconate kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_006677</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-gluconate + ATP = 6-phospho-D-gluconate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19433"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18391"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate acid metabolism; D-gluconate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the gluconokinase GntK/GntV family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.12" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000318">
        <property type="protein sequence ID" value="KAF2072014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5M3"/>
    <dbReference type="OrthoDB" id="275177at2759"/>
    <dbReference type="UniPathway" id="UPA00792"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046316">
        <property type="term" value="F:gluconokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02021">
        <property type="entry name" value="GntK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031322">
        <property type="entry name" value="Shikimate/glucono_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006001">
        <property type="entry name" value="Therm_gnt_kin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43442">
        <property type="entry name" value="GLUCONOKINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43442:SF3">
        <property type="entry name" value="GLUCONOKINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01202">
        <property type="entry name" value="SKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2072014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="188" mass="21462" checksum="43A255241AB72CE7" modified="2022-05-25" version="1">MIYIIIGPSGCGKTSIGVELSNRLDCGFNDADEFHSIENITKMKNGIPLNDDDRKPWLQSINKRILDIIDYQKNNSNSNSNGNEELFLKSKKDHVFTCSALKQIYRDMLTQGINGLDTLTIVYLKGSQELIQKRLESRTNHFFNPSLLTSQFDILEEPNQSLTTNYRIQIIDISDTIDNIVNKIISFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5V1</accession>
    <name>A0A8J4V5V1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003530</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000111">
        <property type="protein sequence ID" value="KAF2075182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5V1"/>
    <dbReference type="OrthoDB" id="444265at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050923">
        <property type="entry name" value="Cell_Proc_Reg/RNA_Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23308">
        <property type="entry name" value="NUCLEAR INHIBITOR OF PROTEIN PHOSPHATASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="114"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="366" mass="41688" checksum="64417F4CBCFC20EC" modified="2022-05-25" version="1">MLSIANLINPAMSQSSSISFFDQSTLEILDITVEYESHLLKNDDHQQLQQQQQQQQLQQQKPQQKQKLNNYTSFQCPIWVCPIMKPERNDHLLFFQNGVKNTSFVVDLEEFKYIILGRSTSVCNYVLSHPTVSRKHALIAHDIEGRLQIMDLNSLHGTYVQEKRISPSMPHTLYGGEKIQLGEDTSFFLVSKRFNQNKHTATLLLEKKQNTNSDVDVVNEEILKFFSHINKTDALGDNDIDDIKSTIIQLNPKLTLGKRKQMDNCKAKRSVVINEIVEKFIFSSQDEISNQTTSTKTPINYVLNNNSISNFPISSPTKPKKSNINRSTINLTSNKRQCLFTSSNSIILPYQTNPSSTTSTTTANEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5X0</accession>
    <name>A0A8J4V5X0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_006491</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the BMP lipoprotein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000302">
        <property type="protein sequence ID" value="KAF2072194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5X0"/>
    <dbReference type="OrthoDB" id="18051at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15047">
        <property type="entry name" value="7tmC_GABA-B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051530">
        <property type="entry name" value="mGluR/GABA-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003760">
        <property type="entry name" value="PnrA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46924:SF2">
        <property type="entry name" value="METABOTROPIC GLUTAMATE RECEPTOR-LIKE PROTEIN A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46924">
        <property type="entry name" value="METABOTROPIC GLUTAMATE RECEPTOR-LIKE PROTEIN C-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02608">
        <property type="entry name" value="Bmp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035246622" description="G-protein coupled receptors family 3 profile domain-containing protein" evidence="14">
        <location>
            <begin position="25"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="382"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="417"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="449"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="489"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="541"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="582"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="606"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="15">
        <location>
            <begin position="380"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="644"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="644"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="658"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2072194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="705" mass="79435" checksum="05D99C14D9928BCF" modified="2022-05-25" version="1">MMKYFSFIIVLLSLVAYNVRFGDAIPCQIALFLSGDFNDMGYNYMMNEGRVRTEKNLKLTHTMYYSNLEGEDEKTGEAIEEAIREGSNLMIISSQVHATIGIQYARKYLDANIFWLIRSKSRPTPDNLPNVAILNYNSAQLQYLLGYFAGLTTKTDKVGFVSPGYWVQKLLTVNSFYVGAKRANPNIKFYSVYTGSWFNPDVAARASLELISQGVDLLGMSQDDMTVQTTVIENNGTFGLGATGYPERLIYGEAIGMSYITNWTNVFTHYAETIINNTWIPYDVYNANIKDNTLQMDSFSYRVSQTVQDRFNAEYNLFKLDNTYRPFMCDPGYDVEYTLDSKGCVKTEAEFYGNSLLLPEIEDFGLYTVPLEFVPYPDSLKYGLSITAGICIFCTLASMGVVLYYNNAKVIRSASPTFCIAILIGCLFVFIGVILWAQNPTDNLCRSKIWMISLGYTIMLGNLLVKNWRIWLLFDNPKLKRRVLTNFKLFPWVGGIMLLDILILALWNGLGDITATKKPGVDGLGKYEYLDVCTFNQKGDIMLYILLIFHGIMLLVGCFVSFKIKVVDIDEFNESKPISTTLYSIAFCLFIVIPLMVSPQSNANQSIIICACAIFTTMSSILILFGSKFHKMIVEGPAINETFRSSSNTKNSETFSGNDHEMEKKEDKEETKTVATTAPKSITISSAIDDSNNQIKSDIEEDINI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6P9</accession>
    <name>A0A8J4V6P9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002616</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000074">
        <property type="protein sequence ID" value="KAF2076052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6P9"/>
    <dbReference type="OrthoDB" id="3219396at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="4"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="177" mass="20890" checksum="114019EC053394E0" modified="2022-05-25" version="1">MNNSLNILDLPNDILEKIFINLNINDWYSFSQTCQEIKSIGNSNLIWRKYCKKEIEFNQTKYNELSLSSYKTFFFKYLVELHFTPNILIFNTSFHLKIKLPLNKPMEELLKIVKKNIGLCRHNSNHGLCDKHCKNLSWPKYILSNSKGMIYDRSKTPKEIGLNDNDTMILSSRLMVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V853</accession>
    <name>A0A8J4V853_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000028">
        <property type="protein sequence ID" value="KAF2077477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V853"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="149" mass="16943" checksum="D4163208A37AA8C9" modified="2022-05-25" version="1">MESDNNKIKNNNNITNSGYIITNSNNSVNDQNHYDNKNQQTIGSVECKPYPYSTKYKHSLVYGGPNKSGFKQQEYMSTFKYKTIKKNKLKVESSLANLFPSQILISPKISKSLSFERSLSKNKKSFVIDSLIKVVMEDDQAEKNSGIDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8P0</accession>
    <name>A0A8J4V8P0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_000726</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000014">
        <property type="protein sequence ID" value="KAF2078002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8P0"/>
    <dbReference type="OrthoDB" id="4703at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052416">
        <property type="entry name" value="GTF3C_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15052:SF2">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15052">
        <property type="entry name" value="RNA POLYMERASE III TRANSCRIPTION INITIATION FACTOR COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="37"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="71"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="91"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="163"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="193"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="240"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="274"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="330"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="345"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="391"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="433"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="445"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="503"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2078002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1098" mass="122540" checksum="CEBE6A8812083248" modified="2022-05-25" version="1">MGPKKEDKSNNNTEGEKEAVVDTPSKPGKPRAKKTSARPSLQTNSIKNYFSVLPTPTTSATKDTDIEMKDNTQQSTTTAIMTRSRTPTKKSNTDTEKEEKEKEKEKEKSDEKSKKEREHLYQRRSKESDYSGSDYSDRDTPKKSKSSSRSSKKKSTSRKSSKSSKDKDSKSSKDSKRSKSSSSSSSKKKYDSSDYSDYSDYSDYSDYSDYSDYSDYSDYSDYSDNDDKYDKKKKGKYYDDYSDDYSDDYSDDKKSKSKSKDKTTPSKKKDSSVKKTPSKSPTKPTPATAKAKTPAKSPSKPSSTPRKTAASAAKPVGSLDSILTKTDTTTGDKEASDDKDKKETTAATTDDTTETTTTTTNGTTPKPTPTSKKTSAKKAATVATPSRKKQKVEDEAQGDKVDDNNDTTPAPPPQTPTTRSSKSGFKPIPIEFSEGDEEVSDSDVDFQSQSSDDSVYSTPPQDEKYSESENEQDDIEPAFTPGGKRKKPTKAAPKQNGTTPTKRPPKPKAAKVPKKKPAASSLEMVSGGGDTRLAVATKPLSQVDYIKNPIKKGSESELENCKIDALPRTPVLTSPVFLPTNYTVLSQEEYEHYLPQEQTSIACKGIGSGDAAQTLPLFGNVTIGKDKDFMIYCGGTVTAMDWCPQVVDEEYQYIAIATHKNTSVTHLKRQIYQGKNIIQVWRVWMKPKLTDEEIAKEVFSKDKDKEKKKQGTSLNTEPKLIMAIAHQGDCCWDLKWIPSGGYSREHNRLGILAAALGDGSAHIWSVPHPDSIVSNNSNSKSKSKEASPSPVVLNIVPFFNYIVNLNRQPNQELLSTPIVQKEFYPTHLSWTQSPNHIKSPRILIGCSDGTVLMFDISKIDNNSNNNNNNNNNNIQQQYPIFINRENDQVSGVSFSPIDDNVFSISYKRRIMVHDLRLNAPIFSFSLHPFSSKFTSFWPRTVAPVIVFSEQNSTKLIDLTTSLTSSVCCHENEINSIDLSRDLNTFASVSTDGLARIYPFFSDRRKYSDNNVIELENLKVENNQLELLKKEKRTTFIIPKITQNPSKTLLSFKALESIYFPDYKLSLQKIAWNKDVRFEKWLCYGGTLGFVRFKTTSNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8P3</accession>
    <name>A0A8J4V8P3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PIPK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003445</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000107">
        <property type="protein sequence ID" value="KAF2075269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8P3"/>
    <dbReference type="OrthoDB" id="2129491at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00139">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF30">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="3">
        <location>
            <begin position="252"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="142"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="85"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="102"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="143"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="626" mass="70718" checksum="3AE6EBC6CBA81B51" modified="2022-05-25" version="1" fragment="single">NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNYMSVKRSSRAISKSSSQIGNHRGSSKSNQKTYSPPSTPTNKLSILDEDEDEQQDKKEKQEQQPVDKEKMLTNSKSLSLPDLSMTNWNNTTPSYSLSPSTSPGSPCLEGVAFSPSCSTSPSISESSSFSSLPPFTPTSPSTLYNHDQQQQQQQQSQQQVKQLDLNGEIYFQDYKDALLNNLLYVKSLGLIGGIGEDGITTLTNSNSLTGSTRNRYQEELTKKWMNSEGREITLGHEHWDTMQYIMIGIRRAIGEAIALPNRPLKQKDFDVTAEFRYNDWVFKDCSPLPFKKIRDIMGVDTKYFMFSLGPEKLFGNLLLGSLSVLCEVVSSGKSGSMFFRSVEGRYLLKTIPSNEEQVLRAMLPSYTSHFEKYPNSFLTKVFGCYNLVNKSKETKMIVMNNLFFTPLPIHEKYDLKGSTIGRHVEVPTGDQDSKIAVIALKDLDFKRKLKIGPAFKAPLLEQIEHDTKFLESHNICDYSLLVGVHRIDENSPIALASDTTASSAFDLIEESFFKKSKSSTGSKTVPLFSKNYGGILSQDQKEVYFIAIIDLFTTWDFRKKYENMFKSLVHDSTKLSAIEPLSYRKRFQQFVSTIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJA1</accession>
    <name>A0A8J4PJA1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010324</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001039">
        <property type="protein sequence ID" value="KAF2068350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJA1"/>
    <dbReference type="OrthoDB" id="24009at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF173">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="582" mass="65487" checksum="958C509C3F148591" modified="2022-05-25" version="1" fragment="single">SSLRFNIETGTLDYMDRFPNYNIFVDDEDQLIPALLQFDKAQQMTISCLSTNLTENIPRSIQRLRCYYDKELIIPSWITDLDILECADLPAVKVPPSVTRLTLSSTLGQSLCEIPATVKYLSCDRLDGPQSIPPSVTHLTLLDQAINTLEIETPSTVQKIEFKKPIPSNATHLFWADDDHFIDNDEVNIPPSVHTLVLGTHFQAPIVSLPPTITQMVFSGELEQPLDTINFPPSLKYLYFNDSFLHDIYEDDLPNGLTHLVFGNFGGQIESLPQSCHHLEFDYQGSSDLDDCNVIPPQIKHLKFTSDYPIKIPPTVKSLMTFSHDQITFIDTLSPPLSQSWSNVSIKEPSPFFLTTRIHFRSSQYSNFNTFIMPNRLGNNIRSIAFGDMFNQGIVKGTFPNSIEHLDFGNKFNQKLKRSKLPTYLLTLVLGSAFNQDLNDVFPSTLTEISLNMLSKPQIKSSSQFPPNLKKLTIPCYDGVLDIVPTSTIHHLQFNNNIVQNATDSLIFPVELTPPNIKTLILNDNLYIESYSIIPPTIKNIKLCLSMVSDTKIPCTIESVVLPALFNQSLDTILQTADGNDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJY9</accession>
    <name>A0A8J4PJY9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Exocyst complex component Sec6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_010313</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001033">
        <property type="protein sequence ID" value="KAF2068362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJY9"/>
    <dbReference type="OrthoDB" id="190098at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051601">
        <property type="term" value="P:exocyst localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.70">
        <property type="entry name" value="Exocyst complex component Sec6, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010326">
        <property type="entry name" value="EXOC3/Sec6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042532">
        <property type="entry name" value="EXOC3/Sec6_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292:SF1">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06046">
        <property type="entry name" value="Sec6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2068362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="741" mass="85588" checksum="4CEDE24032C926BC" modified="2022-05-25" version="1">METSLIPLEGLDDTISQTVAIKKIEAHFANIDSLASVTNHKLSLLQQKKTIEAQIKNEVQSELERSKKGLEILHKSNSRISKMDQSFGVTVTLCQQTASLIGYYPLIKKVNTVRVNLMSILKEVDRLLTIPEKAAEIETLLENDINILNVHKKVRELEILHQKALKQFESNFEELEAIKEMFSSVPELVYRFENKIWDLVSNSVGVSQKQPAVLVKVAQIIEREKLYEQKQREKKKSSSAVLIASDGMEQEEQDRNKTNYGDRFIEVIHQSIASRFEPMFLTCHTDLVATLREVNKMVDELNVVMDQVQECYPSSYDLFNFYVNQYHTKFYSLFESFSHLVESGVNNNEYLAKQIPSAHILMLIEWVVKDYSRQLSRLGIMDLHPPLLDSLDPLIKIYKLHIKGLMKEWCDNIINNDNQNRPDVADGQYFTLAPVHLFESVNSQLDIANATKCQKLVVGVMQEVVSALLYFQFSSMTLLEERNHEIKLENVIAYVNNNSKSYDHTQTIVDKVSNILDTEHMTFLDFDPVLEGFLSVSKVATTAIGSVIFRDLEDTIKKFYTPTWYQEDLMQPIINTFDDYFTNDIQKYILENYMKRLALLCLDNLIEQVLVQLITGKNKFNETTYKIMSNDCDKLLDFFKKYLRLSVVTARVQILEDFKQIATSNADMVPIYFRSIINFHKDVNERVVECILYQRTDVTKAEINEALAQIKTIVETVNTDPNNPPQGIFSRMPIYNRGWFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKG9</accession>
    <name>A0A8J4PKG9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_010182</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000959">
        <property type="protein sequence ID" value="KAF2068493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKG9"/>
    <dbReference type="OrthoDB" id="10046500at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF10">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="7">
        <location>
            <begin position="66"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="15"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="24"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2068493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="144" mass="16226" checksum="7482C10BA2857A94" modified="2022-05-25" version="1">MPSVLLPLLPQPLMPPPNIPLGDATSTARDSPSSATELDSDESFELDLYENSNLSEFQQYVLLVNCKVSPLKWWAVNATEYPILAKMARHFFGATPTEVSLERDFSFGSHLMNPRKAALLPETIRAHFLLRSYLNFIQDFNKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKJ5</accession>
    <name>A0A8J4PKJ5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SUN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_009818</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000818">
        <property type="protein sequence ID" value="KAF2068859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKJ5"/>
    <dbReference type="OrthoDB" id="342281at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045119">
        <property type="entry name" value="SUN1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012919">
        <property type="entry name" value="SUN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12911:SF8">
        <property type="entry name" value="KLAROID PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12911">
        <property type="entry name" value="SAD1/UNC-84-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07738">
        <property type="entry name" value="Sad1_UNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51469">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="231"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="SUN" evidence="7">
        <location>
            <begin position="538"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="69"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2068859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="758" mass="86300" checksum="D05FF43C462EB0C1" modified="2022-05-25" version="1">MNTNYQPQYGATSPGRRRVDVQSNGSPSIYRYGLTNPSTTTTHTVTTTRNSGGQTRATNMLQNHLYHNHQQHHISSMSDDDDENDVQFDSDHSNHQHTRYLQQQQQQQQQQKINTITTANQQQYTTEFRKNVLNESRNPRKLKESLYLSRCSTGSDDSYNNSFENTNQFNNKSYLKDKDKQQQQQQHSNQLVDNSKNNISTTITTTSSANKSATTNPKYKNIASNIFGNNVLVMVTAIIIFFAFLGFLDLSFYYIGGRFLFFSRYLNTTSITSSSTASLSPPTSISPLAIQEQLPIISSFIENSKWSSNIQNSLLDQHNLIKELTNSQNIIKQEIVGHQQLVQDLKREITSMKTTIERVSTNRDIDIDSLKTEIQSFVQQAIGELETRVGNHKDKVTEYQVQSIRSLETKYQSIVDDLDKLSTKQLSVTTQEYSKAQQHWEHHLEKLKVQLESLGKELTHSLVSNIQQEYQQEHQQANQKLNSLFLSIQNKLDRVQVFINENPGITSLSNSISSLEDLLEIYSSDKIAKPDYALYSAGASIEYNSIFKISKTYPSSTGTSLFSMPTSWFIPTPKPNPPETILDPKVITGSCWGFEGGNGTVVIKLSERIRITEVTLEHISPNIAHHINSSPKEFRVIGLKNISDHGTDLGVFTYDTSKNRHLQTFEVLNNVEEQTFSHVVLQVLSNYGYKYTCVYRFRVHGYPIPISIQNEITTTTTDDNNIDNDVSDDNSNINESEHLTQQIVETTFQEQDQQQLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKK9</accession>
    <name>A0A8J4PKK9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">RING zinc finger-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_010106</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000926">
        <property type="protein sequence ID" value="KAF2068568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKK9"/>
    <dbReference type="OrthoDB" id="1923159at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16618">
        <property type="entry name" value="mRING-HC-C4C4_CNOT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12438">
        <property type="entry name" value="RRM_CNOT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000006">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000416">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 4, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034261">
        <property type="entry name" value="CNOT4_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039780">
        <property type="entry name" value="Mot2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039515">
        <property type="entry name" value="NOT4_mRING-HC-C4C4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12603">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12603:SF0">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14570">
        <property type="entry name" value="zf-RING_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="9"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="118"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="62"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="234"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="697"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="741"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="803"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1086"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1195"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="87"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="240"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="270"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="315"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="331"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="388"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="400"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="446"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="558"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="570"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="705"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="750"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="767"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="820"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="834"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="855"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="895"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="907"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="921"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1158"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1198"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1211"/>
            <end position="1220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2068568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1256" mass="142177" checksum="6F5AB23B839F53B1" modified="2022-05-25" version="1">MSDDGDNSCPLCLDELSKADRKFRPCPCGYQICVFCFERIRESEQDRCPACRKNYDSANYSYLEDGSSEEEESEEEYDRRKEKSNKKYNNSNNSLSNSLENAKRGDKPLNTVRVIQKNLVYVTNLALSIAKPELLKKNEYFGQYGKILKIVINKNNIYNVNSPHGACVSAYITYQRKEDALVAIQTIDGATIEGRVLRASFGTTKYCSYFLRKLPCNNPDCMYLHELGQEDESYSKEEITSPISSSTNSTTSTYKPKPNWKESNEKIPISLSNPSTPTTNYTSNSNTSNSSLSHSSSSTPTSSSNQPFKTWNIVAPTSPQTSLGLNPMEWPSTTDSLTMAPANNSPPLPLSKSQERVLPATASWGSKPKTYSSGGDYDEDPPLMKATFEIKTKKKDSLKTSQNSSPSTLSPSINTSSSSLNTPSLSVNTSASSPISSSGTGITVKVDPKIKKEEEKKEKERLEKERLEKERLEKERLEKERIEKEQRLEKERLEKERLEKERLEKERLEKERLEKERLEKERLEKERLEKERLEKERLEKERLEKERIEKEKLEKQQKKQQQKQQKKSNNKNTSTSSTPTLSSTTNTNTTNNTTPTVTSPITTSSNTVATVSPTTTNSNTSASNNQQQQTDLNINVNDIEPIASWEGLISDEQVGDFDWYQPKTLKVDPIKSEILDFIIPEAPTTVVGKSEGRSRFGFTKEKDSQQQSTSSTTSPLITPDMFLGLKSEPYQYPIVSSLHHPNGVDHLHQQQHQTISTPPSFDPNHMLNNNNNINNNNLQQQNTDEDSSQQFFRSLFPSVNIRFSFPNSTSPPPPGIITDNSNGNGSSKGFNDYLDQSNGRNSSYSNDQKDNVWRNSNNSINNSFYNNNNNNNNNNNNLRRSISSSVSPPPGFISFDSSNVQQSSNKPLPPPGFAPSPPPGFQNLQQQQQVQAPPGFHNNLSNSSSGINSNSISSPTTANSFNNSNNNHQQQQFNIQQPQPQTTPINLANYMMDASLLSELEESDLLEKNEKEMKMKNKKVYSSDEIEKQFQQQLNQHPPPQQPQQQNNNHINNLHHQLLQQQQQQQQQQQQQQFLQFQQAFQQQQQQQNVGAKRNDDLFSPLGMPQFMPPPNSNMLFNSFLPPMHPSQQPKQQAPPPFPPFNFNHPPNGAPRVGGGFPSSNQPQPQSNQQQSNQQKTNNFFDFLTQNGITYFRNEDLNNNNNNNNSNINSMYQPQQSTDSLEQRVANVNKEMKELEEKLQKATLNNNNNNSIPYYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKM5</accession>
    <name>A0A8J4PKM5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyldiphosphate specific]</fullName>
            <ecNumber evidence="5">2.5.1.87</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_009923</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>n isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate = a di-trans,poly-cis-polyprenyl diphosphate + n diphosphate</text>
            <dbReference type="Rhea" id="RHEA:53008"/>
            <dbReference type="Rhea" id="RHEA-COMP:19494"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:136960"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.87"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the UPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.87" evidence="5"/>
    <dbReference type="EMBL" id="AJWJ01000855">
        <property type="protein sequence ID" value="KAF2068753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKM5"/>
    <dbReference type="OrthoDB" id="19639at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904423">
        <property type="term" value="C:dehydrodolichyl diphosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038887">
        <property type="entry name" value="Nus1/NgBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21528">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT NUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21528:SF0">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT NUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="34"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2068753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="364" mass="41755" checksum="2F8E73BBF2BC133A" modified="2022-05-25" version="1">MVIVSTTKTSKTVLIHKNESTNDQKLTIKKLQIHQPTQQQQNSNIDSNSNSNGSSNSSNSNNIKDTKRQQQYNHQQQQQRKEKSLYSFITFIIYHSIFYIGVGVRNLYEYYSIFTLKIKALIFQILLSFQVKTPEDYVNYIKYRLNSDSLVVPKHLAIILNHHDQVQSTQSQLDNKIFYNKLTEILVWSIIVGVHRVTIFDNHGELKKNISYLQQLLLKRIGPFNNNKKSSSSSSSNGTTSTGKYIFNWLNHDSTNHSNNSNHDNSKLNSLLDISVVAIEDGKQELINITKKFVKEQLDQQVLNNNNGDNSNNKIGVINEAYINQNLSRPIGGSLFEPEAALDFTGQYFFAGFLPWHIKLTEFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKY2</accession>
    <name>A0A8J4PKY2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000842">
        <property type="protein sequence ID" value="KAF2068793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKY2"/>
    <dbReference type="OrthoDB" id="193045at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40535">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_9, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40535:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_9, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035285885" evidence="1">
        <location>
            <begin position="22"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="315" mass="36205" checksum="B6988CB2E93E501D" modified="2022-05-25" version="1">MNRNILLICITFIVLARLGLSIENYQLSSSVEEFLCTIDKYLNIDYNLLENSDIPYYSQEQKEKLENIYRDALKRIKDRVLHINSNNNNADNIDQYEFDPPIYNSTVYDAFASKTLLNPQETLDYNPYDINDFNTTLSNVSVCGVLYPNENDRSTYYLVNYTSAQEAQEAGAYVTHLHSCGYCSTTQDLAAYMKYMDLTSPIRDCAIVSFISKEISLDCIQKVAQFSHSCSIIWLYDSLNTRKYCLDICLYDYIHHVPNNVPTNSTILNDCLACDEEKSGPVFKVVAGRTRRDSGLKSAINRPPDSIYNITHYYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKZ7</accession>
    <name>A0A8J4PKZ7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptide chain release factor N(5)-glutamine methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_008914</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000598">
        <property type="protein sequence ID" value="KAF2069762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKZ7"/>
    <dbReference type="OrthoDB" id="269872at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004556">
        <property type="entry name" value="HemK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050320">
        <property type="entry name" value="N5-glutamine_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040758">
        <property type="entry name" value="PrmC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019874">
        <property type="entry name" value="RF_methyltr_PrmC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00536">
        <property type="entry name" value="hemK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03534">
        <property type="entry name" value="RF_mod_PrmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18895">
        <property type="entry name" value="HEMK METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18895:SF74">
        <property type="entry name" value="MTRF1L RELEASE FACTOR GLUTAMINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17827">
        <property type="entry name" value="PrmC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Release factor glutamine methyltransferase N-terminal" evidence="5">
        <location>
            <begin position="39"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Methyltransferase" evidence="4">
        <location>
            <begin position="152"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2069762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="322" mass="36897" checksum="E2E8C7E24AD153A4" modified="2022-05-25" version="1">MFFSKNQRVKKSFIKILVNHFTSSSSSINNKSLKIKDVLKETRDKLNTCSNKSIVDSSDLDSRLLVSDLLGFQDSKSILLLNRKQEIDSQQYEKLQSFINKRLQSEPISYILGYKYFWKHKFNCNSSTLIPRPDSEILIETILELKNEIDIKRVLDLGTGTGCLLLSVLDEIKDSQGFGIDQSNDALDVAKQNSIDLQLSTRSTFINANWNDINQFPVLEPFDIIISNPPYISFDEYNTLNDTVKKFEPKSALIAKENGLQDYRIIGSLIRSRKLLNPNGFIVFEIGKDQHLDIENIMKDFGFTLYLSKKDLSGIIRCLIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL47</accession>
    <name>A0A8J4PL47_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008737</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000569">
        <property type="protein sequence ID" value="KAF2069940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL47"/>
    <dbReference type="OrthoDB" id="1607513at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20916">
        <property type="entry name" value="CYSTEINE AND GLYCINE-RICH PROTEIN 2 BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20916:SF26">
        <property type="entry name" value="CYSTEINE-RICH PROTEIN 2-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="344"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="695" mass="79893" checksum="8779E78E301CEB67" modified="2022-05-25" version="1">MNVNSKRPNDVDNSNNSNGGYSNSYPQQQLAKRLKVNNEYLQLFPTAEERNTAKDLFCKDENSAYNYFDFDSGLCKLCAEPLAIIKKKDKKILGRLSNFKTHLEGVHSISHEKSSKISNKNIITTTPITVHNLVENDTNQQRSLQQYNYEEDFDNNNNDSNDIYDDIENIPIHVGPSKSLDNNNNNNNNNSKQSTIINDILPEKILIKMDLKKMAYMSLTHDIWTNRGGFNQSFFSISAHGLVEENNLLIRKTFTLDTYDLFFERQTSKQHLASLIKQTMDKWELDPTKVYFCIGDNDKIALEFVNEANLKPFICLPYSLQLALNPLIENNTDSPDQCEKEENSNNNNNINLNQNTTLEDLENISIADLKMYYNNNSSAITTAVKKRVALNKDEIISQLLQFIKTAPFKQILIRQLKKEIKASQDKEPFVVARGLIKKFLRLKEFMNQSSKNLNLSDLLPSLDLETTFMTKWRNTLQGLDVFYKTFDFIKTNYPQQGEEISAILTKDSNNQLLDIIDILKLFNNAIERFSKEDQVELSLVYIEILKLTSNLDNSSKKVYQSGLGVDIAMELSNHIHRESCQFLGSPQAQIATFLNPKYFQDLARLAGKEKFKEIRDLVKKEANIGNDEIMDDDGATEIHAYIVLSRQQGITDPLEFWLQYKKSLPQLYDLVLKYMSIPSVINSPIPLERIFSNNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLK3</accession>
    <name>A0A8J4PLK3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ComC supersandwich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009568</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000745">
        <property type="protein sequence ID" value="KAF2069110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLK3"/>
    <dbReference type="OrthoDB" id="21152at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF5">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="2">
        <location>
            <begin position="4"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="255" mass="28469" checksum="0FF2A7A620AF51FB" modified="2022-05-25" version="1" fragment="single">IVLINEVDPNNNVIQSYNISSISWNNITKQDSIYSYTTTLPNTKSTLNVQLTFNPLNEHMYVDFAGDVIPILPKSIKYQVELQNYTFSSSLNTMEFVFKSGIIDKGDDCVYDESTTTKKSLDSIRSIQMTLNGETLIGTFSDRIVLDDRPSYNKVNQLTDDQIIKYQLNQESLYVSIATTYFKSSVVVDPNFGVLVSSNPDSNACKKGFASWKIGVIVGCSVIGVGAVAATTMLIKKKRVQAKFDNQMKNMNKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLQ0</accession>
    <name>A0A8J4PLQ0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">2-oxoglutarate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="5">1.2.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">2-oxoglutarate dehydrogenase complex component E1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_009435</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 2-oxoglutarate + H(+) = N(6)-[(R)-S(8)-succinyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:12188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:20092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83120"/>
            <dbReference type="EC" id="1.2.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the alpha-ketoglutarate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.2" evidence="5"/>
    <dbReference type="EMBL" id="AJWJ01000708">
        <property type="protein sequence ID" value="KAF2069243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLQ0"/>
    <dbReference type="OrthoDB" id="413077at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004591">
        <property type="term" value="F:oxoglutarate dehydrogenase (succinyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02016">
        <property type="entry name" value="TPP_E1_OGDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12470:FF:000003">
        <property type="entry name" value="2-oxoglutarate dehydrogenase E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000002">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11610">
        <property type="entry name" value="Multifunctional 2-oxoglutarate metabolism enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1150">
        <property type="entry name" value="TPP helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032106">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011603">
        <property type="entry name" value="2oxoglutarate_DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042179">
        <property type="entry name" value="KGD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031717">
        <property type="entry name" value="ODO-1/KGD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00239">
        <property type="entry name" value="2oxo_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006914">
        <property type="entry name" value="PRK09404.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008907">
        <property type="entry name" value="PRK12270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152:SF4">
        <property type="entry name" value="2-OXOADIPATE DEHYDROGENASE COMPLEX COMPONENT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152">
        <property type="entry name" value="2-OXOGLUTARATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16078">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16870">
        <property type="entry name" value="OxoGdeHyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000157">
        <property type="entry name" value="Oxoglu_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="16">
        <location>
            <begin position="635"/>
            <end position="851"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00040267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2069243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1008" mass="112947" checksum="E951AB3EFDEACC0A" modified="2022-05-25" version="1">MQSLTKHTLKQALKLSHRSPVAIRSFSTVNTPKSKKELSESFLDGTSSVYIEDMYNSWKKDPTSVHKSWASFFSSTDGGAAVGEAYMSPPTLGSSTATKAAPSSGASPSAVNLQSISDSMKLLLLIRAYQVRGHAIATLDPLNLEVRPEPAELKPSKYGFTEADMDRPIYVGEGLISGFLTNNAPQTTLRQVLNRLKEVYCGDIGIEYMHIQDREMCDWIRENFETFTHFTLPKEEKIKILERLSWADQFEGFLGLKYKTQKRFGLDGCESLIPGMKAMIDTAAGNGVNQIVLGMPHRGRLNVLANVVRKPLPAIFNEFNGGVISIEGEYSGTGDVKYHLGTSYDRVTADGNKVHLSLVANPSHLEAVNPVVEGKVRAKQHYANDKQHEHSLAIQMHGDASIAGQGVVYETLHLSQLENYTTGGTIHVVVNNQIGFTTNPNCSRSSMYCTDVGKTIDVPIFHVNGDNVEAVVHVCKLAAQWRQKFQRDVIIDIVCYRRHGHNETDQPKFTQPIMYDKIAKQTPVIEKYSNQLIGENVISQEHYLQMKNIIHEAYEKGYQEGMKYSPKATDWLESKWEGIKSPIEMGTTFKSGISEELIQSIGKVLYTEPKDFELHSTIKRLLKEKKEMFVKGSGFDWATAEALAFGSLLLEGNHVRLSGQDVQRGTFSHRHAVLHDQKTDATYTPLLNLSKEFNKDAAEFVASNSSLSEFAVLGFELGYSLESPKSLVLWEAQFGDFSNGAQVIIDQFISAGEAKWMRQSGLTMLLPHGYDGAGPEHSSCRLERYLQMCDSDPNKVYQAGQENITKQAQHSNWQVLNISTPANYFHALRRQVHRDFRKPLVITTPKYLLRLQQSFSNIKDFTTEGFQRLLPEATPEEINAPEKVQRLVFCSGQVYYLLAGARAQAKIKDTAIVRIEQLHPFPFDLVAEQMKLYPNAKVIWCQEEPMNMGPWNFLFPYFHSTFKSIDRPFNITYAGRPASASPAVASHSLHKLQLETFLSEALGTKISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLZ2</accession>
    <name>A0A8J4PLZ2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010057</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000906">
        <property type="protein sequence ID" value="KAF2068616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLZ2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="42" mass="4922" checksum="E2D2592418F49BD9" modified="2022-05-25" version="1" fragment="single">MMEISVPSMTQPGRAQAYGKTERTNRTIKEQIIKEANNRKKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMM9</accession>
    <name>A0A8J4PMM9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PRA1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_009013</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PRA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000618">
        <property type="protein sequence ID" value="KAF2069665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMM9"/>
    <dbReference type="OrthoDB" id="18213at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004895">
        <property type="entry name" value="Prenylated_rab_accept_PRA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19317:SF93">
        <property type="entry name" value="PRA1 FAMILY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19317">
        <property type="entry name" value="PRENYLATED RAB ACCEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03208">
        <property type="entry name" value="PRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2069665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="154" mass="17068" checksum="ED794E5A7C93F7FC" modified="2022-05-25" version="1">MEKLNIQPWSGFGDWNSFSVPGNQLKDRVENNIQFYSGNYAIIALVVLLLTLFTNFNLLIAVVILGGIFYVTCIQDTGNLRVGNFVLTLIMRMAIIGVVSIVTIYKISGMTLIYTSAISASAILVHAVFRKRTLKGKINATANKANDFVDRNFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMQ8</accession>
    <name>A0A8J4PMQ8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PhoPQ-activated pathogenicity-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008600</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000546">
        <property type="protein sequence ID" value="KAF2070074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMQ8"/>
    <dbReference type="OrthoDB" id="2020799at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009199">
        <property type="entry name" value="PhoPQ-act_pathogen-rel_PqaA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31497">
        <property type="entry name" value="AUTOCRINE PROLIFERATION REPRESSOR PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31497:SF0">
        <property type="entry name" value="AUTOCRINE PROLIFERATION REPRESSOR PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10142">
        <property type="entry name" value="PhoPQ_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF014728">
        <property type="entry name" value="PqaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035168377" description="PhoPQ-activated pathogenicity-related protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="481" mass="55022" checksum="3C3219B0FDCE52F5" modified="2022-05-25" version="1">MKNLVTLFVILSVTLSLTWALNSPLENFVEKPDQGWSYTLNKTLVFPGYSIHILNFTSINWLTSSESNHPRWWHWLSICVPTEVYSKDGFLYVDGGKYDTNVDAPHAVLVKLCSTSNSIVSHLHNNPNQPLVLDNDGISRTEDDIIAYTWKKYIDNRNLTDWIGQFAETKATVRAFDAIQAFVKATTPYRVKNFVVAGESKRGWASWLAAAVDTRVKAVIPIVMPILNIIENTNAHFKAYGGWSFTYDDYFEQGIMTHLNKEPFEALAKLVDPIRFVEQLTVPKYVVNAAGDQYFLPDSATFFWKDLLGQKHLRIHPNGDHYIIKSRKAQLVNEIDTYYNNIINKGKMPNFEWSIIPDPTTNTTNMFVNIDNKHNELKNIVVYFADTVSKTQRDWRWTTCGTSACFQGIYWNPLPVIKLDDNLYKYTITPPVAGGWRGAFFELTFESPYGDLVYTSDFGYAPYILPFQSCGQDCQNQPFPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN22</accession>
    <name>A0A8J4PN22_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing factor 18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_008520</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRP18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000532">
        <property type="protein sequence ID" value="KAF2070163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN22"/>
    <dbReference type="OrthoDB" id="10261918at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071021">
        <property type="term" value="C:U2-type post-spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000350">
        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.940.10:FF:000004">
        <property type="entry name" value="Pre-mRNA-splicing factor 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.940.10">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.110">
        <property type="entry name" value="Pre-mRNA processing factor 4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004098">
        <property type="entry name" value="Prp18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014906">
        <property type="entry name" value="PRP4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036285">
        <property type="entry name" value="PRP4-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039979">
        <property type="entry name" value="PRPF18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13007">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13007:SF19">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02840">
        <property type="entry name" value="Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08799">
        <property type="entry name" value="PRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00500">
        <property type="entry name" value="SFM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47938">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158230">
        <property type="entry name" value="PRP4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA processing factor 4 (PRP4)-like" evidence="9">
        <location>
            <begin position="104"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="15"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="165"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="76"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="172"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="183"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2070163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="367" mass="42911" checksum="4674D9EA3B974E6A" modified="2022-05-25" version="1">MKSLKEQLQIEKKRKEALLNKDESSINSNTSTADQQPTKKYKTRGEIEKEQREKKEEEKRLKQKLKDSNNNNNNNDIDKDKVKQLDQQEKEKEEEKKKLREPFLPKHIVIQRLRERKQPITYFGEDDYTRAERLRHLEEDDPIEYTKGENEFANILKQIESNQKFKKDDQLDSNSSSNSNNNKTDKDSNGKKDDNNYLNMDLSALFEESKKSKEAGILFFLTGLMREWQENLDNRAEEEKKSRQGKLTEATFVQCLAHIQPLFNQLTAKTLPDDILQHIHGIVESCKAREYVKANDEYLQMAIGNAPWPMGVTMVGIHERSSREKIFSNQVAHVLNDETQRKYIQAVKRLITFCQTQYPALPSKCVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN40</accession>
    <name>A0A8J4PN40_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Histidine kinase/HSP90-like ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_008624</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000550">
        <property type="protein sequence ID" value="KAF2070055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN40"/>
    <dbReference type="OrthoDB" id="28737at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16927">
        <property type="entry name" value="HATPase_Hsp90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.790:FF:000001">
        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.80:FF:000001">
        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11260:FF:000001">
        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000001">
        <property type="entry name" value="Heat shock protein HSP 90-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.790">
        <property type="entry name" value="Heat shock protein 90, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00505">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019805">
        <property type="entry name" value="Heat_shock_protein_90_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037196">
        <property type="entry name" value="HSP90_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001404">
        <property type="entry name" value="Hsp90_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020575">
        <property type="entry name" value="Hsp90_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003555">
        <property type="entry name" value="PRK05218.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11528">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00183">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002583">
        <property type="entry name" value="Hsp90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00775">
        <property type="entry name" value="HEATSHOCK90"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110942">
        <property type="entry name" value="HSP90 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00298">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="10">
        <location>
            <begin position="29"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="669"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="512"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="102"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="123"/>
            <end position="128"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR002583-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2070055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="697" mass="79806" checksum="FF46CED5D37FDEDA" modified="2022-05-25" version="1">MSGQEVERFTFQAEINQLMSLIINTFYSNKEVFLRELISNASDALDKIRFQSLTDPSVLESKPELEIKIIPDKVNKTLTLIDSGIGMTKSDMVKNLGTIARSGTKNFMEQLQSGSADISMIGQFGVGFYSAYLVADTVIVHSKNNDDEQYIWESSAGGEFTVALDHGEPLGRGTKIVLHMKEDQLDYLDEAKIKGLIKKHSEFIQYPISLLTIKEKEVEEDKEEKVAEESTDAKIEEVEDEPKEKTKKKVEEKEWELLNKTKPIWTRNPSEVSKEDYNAFYKSISNDWEEPLAVKHFSVEGQLEFKAVIFVPKRAPFDLFESKKKHNNIKLYVKRVFIMDNCQDLIPEYLNFVRGIVDSEDLPLNISRETLQQNKILTVIRKNLVKRCLELFGEIAENAEDFKKFYEAFSKNLKLGIHEDTQNREKLADLLRYQTSKSGDELSSFKEYVARMKENQKDIYFITGESKKAVENSPFVEGIKKKGYEVIYMCDPIDEYAVQQLKEYDGHKLVSITKEGLTLDETEEEKKKAEQDKTANEALLKEVKEVLGDKVEKVTLSNRLLNSPCVLVTSEFGWSANMERIMKAQALRDSSMSTYMSSKKTFELNPDHPIVQELRKRCTEKSKTFKDFVYLLYETALLSSGFTLDEPSSFASRIHRMIKLGLSIDDNNTSAAEETTASDDVPPLQEDNETSQMEQVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN60</accession>
    <name>A0A8J4PN60_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">V-type proton ATPase proteolipid subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_008584</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Vacuole membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000543">
        <property type="protein sequence ID" value="KAF2070100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN60"/>
    <dbReference type="OrthoDB" id="1744869at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18175">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18176">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.610:FF:000001">
        <property type="entry name" value="V-type proton ATPase proteolipid subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000245">
        <property type="entry name" value="ATPase_proteolipid_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011555">
        <property type="entry name" value="ATPase_proteolipid_su_C_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01100">
        <property type="entry name" value="V_ATP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10263">
        <property type="entry name" value="V-TYPE PROTON ATPASE PROTEOLIPID SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00122">
        <property type="entry name" value="VACATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="65"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="10">
        <location>
            <begin position="26"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="10">
        <location>
            <begin position="104"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2070100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="187" mass="19197" checksum="2ED2974A6F50D1C6" modified="2022-05-25" version="1">MFQLFALLARVITDACPIYAPFFGAMGVTSALVFTVMGAAYGTAKASVGISNMGVMKPDLVIKAFIPVIFAGVIAIYGLIICVIIVGGIKPTESYSLLKAFTHLGAGLTVGLCGLAAGMAIGIVGDSGVRAFGQQPKLYVIMMLILIFSEALGLYGLIIGILLSGAASSDDYCPGIPLPNQPTNSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNA3</accession>
    <name>A0A8J4PNA3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine hydroxymethyltransferase</fullName>
            <ecNumber evidence="9">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_008303</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the SHMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="9"/>
    <dbReference type="EMBL" id="AJWJ01000500">
        <property type="protein sequence ID" value="KAF2070374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNA3"/>
    <dbReference type="OrthoDB" id="10265628at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000050">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF18">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7 8 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="10">
        <location>
            <begin position="40"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="8">
        <location>
            <position position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2070374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="488" mass="53822" checksum="CB8C97EBFB99FF3B" modified="2022-05-25" version="1">MISRLSKITSFSSVQRLASLSSVRSFSTAAKPIAGNKSVEESDPEIFKLIKDEAERQYTGLELIASENFTSRAVLESIGSIFTNKYAEGLPGARYYGGNEVVDKLENLCIKRALDTFGLKETEWGVNVQPYSGSTANFAAFTGLLKPHDRIMGLDLPSGGHLTHGYQTDKKKISATSIFFESMPYQVNSTGYIDYDKMEANAALFRPKLIIAGASAYPREWDYERMRKIADKHGAYLLCDMSHISGLVAGKQAISPFQFCDVVTTTTHKTLRGPRAGLIFFRKTKRTDNKGNIIDDDLENRLNFAVFPSCQGGPHENTIAGVAVALKEASSTEFQEYIKNVRENSAIMAEELKKRGYSVVTNGTDNHLVLWDLRPQGITGSKIEKACDEAHITVNKNAVYGDTNAIAPGGIRLGTPALTSRGLVGQDFVKVVDFLDRVVKLSIDIQNKVGKKMPDFQKAIAGNQDFKELRKEVVEFSKQFQMPGQPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNL5</accession>
    <name>A0A8J4PNL5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Zeta-coat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_008943</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. The zeta subunit may be involved in regulating the coat assembly and, hence, the rate of biosynthetic protein transport due to its association-dissociation properties with the coatomer complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">COPI-coated vesicle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000603">
        <property type="protein sequence ID" value="KAF2069741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNL5"/>
    <dbReference type="OrthoDB" id="10249988at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000013">
        <property type="entry name" value="Coatomer subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039652">
        <property type="entry name" value="Coatomer_zeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11043:SF3">
        <property type="entry name" value="COATOMER SUBUNIT ZETA-A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11043">
        <property type="entry name" value="ZETA-COAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="14">
        <location>
            <begin position="8"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00075766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2069741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="171" mass="19519" checksum="F853600A74E55817" modified="2022-05-25" version="1">MSSFLYTVNSFFILDNKGERVIAKYYSNDFENVTKQKAFEQKVFDKTSKVHFGGEITLLDNYIIVYKAFSNIVIYMIGDSDQNEVALLYVLNSFIDTLQNLFENQINKRTILDGINYTLLTLDEIIDGGIVLESDTSVIVDRVGIKLPDNDEDELGKVISSAKEQFINFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNX4</accession>
    <name>A0A8J4PNX4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000462">
        <property type="protein sequence ID" value="KAF2070658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNX4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="729" mass="85750" checksum="E425D3A4BC41E0D6" modified="2022-05-25" version="1">MLYEVYKQVFGNIFLSTQIFKHVHQLQFYQYGLEYDDIVDIGWMYKHNHKGLLKEKLNNSNNYLYMDGEILFTKIANDDTELFITLFERFKHHVLGYYYRKNDNDWSKYLNNFDVVKYLFNRGYGKRIWRINLNNIDINILKYYLENQWLKPTMDLFSYYQSELMKDNRTSRIDLQEKIEMIVKYIEPTKIGENIIRHPAQLLGSLQDVPIPSLIKSISHLLGNKDDVIMMNDYTFQNQQSITIQQAVENVQQAEDHLVKLVQSLGGVSNENSNIKLNHFKFSRVQTDQAPQRPFWPLWVEKTGQEFITSWKESSKRIRVTAETLCFSNLVNGADHQKLKDEHSSFRDIGPFLVAKFMVNVNVQGLIFIYQQGYHAINMSGIGLHMFYQDLVKLTNKQDREAIVGLSNSPFVLGPHLNPNNIPDQGPNVLSKLEILKRCCDGGYYQNLDYYLGKFKDNLELDQQQINYLFARVRLNRYAIGVLYSHGFIYTDKIYDGVEFYIHFWFDKFDKERMKTFLRRLDKPTKRMLLTQLIECIVINNDYVSFKHLMNNFKNNKLTIDPNRSFLSHLAPATNINIIDYIKKNKSTCLRSNVVFKFFFQNLFEMATEDTDFNIPLIQYLLTNNCVDPDSSPSIPAVLESFRSKTRVINLNHFIYIAHRYKYMDQDANTPLTLVEFISRDQQKCNGKLNFKSIYANYGVDKNREKGLAYNNIGSLLVDLTNTYDTTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP95</accession>
    <name>A0A8J4PP95_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006995</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000349">
        <property type="protein sequence ID" value="KAF2071695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP95"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="5"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="667" mass="78023" checksum="29BFBC6219130641" modified="2022-05-25" version="1">MQDNIDLLEQEIRNNNQIITRLRELKLYIKNRLDNNYLQSLEKELSILENRLLSSFNQLTEKKHNTINYLDNFNSKKSATQLLVQVLDWFKEKNRLFSYNIYSLTKKEYGIIDQLLKSYYKSQHSLTPPPQTDQRQQQFRVKLNECIDIKELIDTRPLLCYPIINSYKFFDLPKHPIVDPINNSQLDLFINSKTTPRSVSENDNNDNSIDNVFVIRYKVYHFTRLFYYISKSKFQFNYLFFVKTLGDYKNILIKLDKNFSHLVIVDDRQLIDLFNSNIRNLTSALIVYHSQFYDKYKYEIQYNYSNIKTIIITSFHSNFKANHPNVNIFGEYVLERLSTEGLELLFNNLTDSIVSKDDIFYKPLMYSITKIPLVFMIEDLTKHIKNKTKTPNEIIYDILSIEANTMAPSTIIPGFKSKNIIFKFPVGEMLWSLKVNYFESQTKQNNEWVPITIMQEPNECTDPYQLILTSEYAQIIINNTNGDIYNIKFDLFFNKACSRFGGECINLIPLSGTFKSPSHGIQSLSLSNCFGSILHRPYMSDERVPKIEIDAIGYSFSKNNNQTSEKRIAIRLEDGEKKINFINIEGNNHFIGDVKFLNDTDYTLIIISNYSRFANNNQLIFKSKDNQPFPDRGNFSGNLCGDIILKDATIISFDISAFLSMGKRNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPE0</accession>
    <name>A0A8J4PPE0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">sn-1-specific diacylglycerol lipase</fullName>
            <ecNumber evidence="14">3.1.1.116</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_008344</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1,2-diacyl-sn-glycerol + H2O = a 2-acylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33275"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17389"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.116"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:33276"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.116" evidence="14"/>
    <dbReference type="EMBL" id="AJWJ01000505">
        <property type="protein sequence ID" value="KAF2070341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPE0"/>
    <dbReference type="OrthoDB" id="438440at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019369">
        <property type="term" value="P:arachidonate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046340">
        <property type="term" value="P:diacylglycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000321">
        <property type="entry name" value="Lipase domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052214">
        <property type="entry name" value="DAG_Lipase-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45792">
        <property type="entry name" value="DIACYLGLYCEROL LIPASE HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45792:SF8">
        <property type="entry name" value="DIACYLGLYCEROL LIPASE-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fungal lipase-type" evidence="16">
        <location>
            <begin position="345"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="653"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="33"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00026104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2070341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="673" mass="75698" checksum="FF221CF403F22B3E" modified="2022-05-25" version="1">MISNSNNNSSNNTSFNKMDEIDEDNNTKFDTLSLNNNTSNDSNSSNSNNSSNSRNINGNHHHDQSDTHHDMLLLSPPPPPPPLESYTPNTHVLHTPTPELLQSPILSTIIDSLNDKMVIEATNSIISNNSINNSGDNNKSSTITTTTTKEIKINEKDGLIEEFQVIDEYNPLIDKDDSKRILKNDKSKPTNFFSNIYSKIKYSIISPETEEALGHINDLLTELFQDVSYSKLQIMCGLLLLDSYYSNSHIKTGSYVKDTEFLKTAQRYMKFANASFGWKYVNGYLYTKKAKGIVQGVVGGDDMNNKCLCEYTGIEKQDIILTKWTSTNFDPGHFLVFDHQNKSVVLSIRGTFNARDVLTDLVAKDTPFYDGHAHTGILKCAQKKFKELMPLILEAIERYPGYGLIVVGHSLGAGVASLFTIISKNRYPEIPVHCYAYAAPCVASSEISLSIEYRSLIDTFVFNDDIVPRLCYHSLVHLKQLVCAILGQNDSLPQLTFQIIAAGNSLGEQLTEKISSLLKVNREKIKYEENMITNKSMLPPGRVYRIYQPDNINYVMEESNPSFFKEIIISNTLISDHMPDKYEKAFVDCISNLNSINPYHYIEEDQDIVILNQDITNDDNKTTSTDIETTVVTTIDPKEETVTKEIKSIKTTTISSNNDSNDNNQISDNIIHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPU5</accession>
    <name>A0A8J4PPU5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Kynureninase</fullName>
            <ecNumber evidence="4 5">3.7.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">L-kynurenine hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">KYNU</name>
        <name evidence="6" type="ORF">CYY_007346</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>3-hydroxy-L-kynurenine + H2O = 3-hydroxyanthranilate + L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25143"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36559"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:58125"/>
            <dbReference type="EC" id="3.7.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>L-kynurenine + H2O = anthranilate + L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16813"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="3.7.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 5">Amino-acid degradation; L-kynurenine degradation; L-alanine and anthranilate from L-kynurenine: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 5">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the kynureninase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.7.1.3" evidence="4 5"/>
    <dbReference type="EMBL" id="AJWJ01000386">
        <property type="protein sequence ID" value="KAF2071333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPU5"/>
    <dbReference type="OrthoDB" id="5978656at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00329"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00334">
        <property type="reaction ID" value="UER00455"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030429">
        <property type="term" value="F:kynureninase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043420">
        <property type="term" value="P:anthranilate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097053">
        <property type="term" value="P:L-kynurenine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019805">
        <property type="term" value="P:quinolinate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000031">
        <property type="entry name" value="Kynureninase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01970">
        <property type="entry name" value="Kynureninase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010111">
        <property type="entry name" value="Kynureninase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01814">
        <property type="entry name" value="kynureninase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14084">
        <property type="entry name" value="KYNURENINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14084:SF0">
        <property type="entry name" value="KYNURENINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22580">
        <property type="entry name" value="KYNU_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038800">
        <property type="entry name" value="KYNU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4 5" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="3 4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="158"/>
            <end position="161"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR038800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2071333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="451" mass="50741" checksum="F9DD03D8ED65A3A1" modified="2022-05-25" version="1">MENFLNYIKQHDLDINDETLAEKLDALDDLSHMRKEFNFPQLGDIATEINDENRDKVDQEVIYLTGNSLGLQGKAVERELKVYLDDWKKYAVEGHMKGTHPFISIDEEIQSRLASIVGAKPSEVCSMNSLSVNLHILLSNFYKPTKDRYKIVIEYGAFPSDLYVTESQLRNHGFNPETDLIKIKPKENEYTLTTQEIIDTLKEHGDSIAVVLLSGVQYFTGQFFDIKTITKVGHECGAIVGWDLAHAAGNVQLQLHDWDVDFASWCSYKYLNSGPGCIAGVFVHSRITETFDLATDNRLMGWFGNKLSHRFHKEKPFVAETGALGFRCSNPSVADTTALRASLDIFEKAGGVSKLSAKSVILTGYLEYLLRNKLASTNIQMITPTDPSQRGAQISLLVREIKANEIKHKLLNSGVICDVREPDVIRVAPAPLYNSFTDCHRFIQLLNKAMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ16</accession>
    <name>A0A8J4PQ16_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">IPT/TIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_007944</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000451">
        <property type="protein sequence ID" value="KAF2070745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ16"/>
    <dbReference type="OrthoDB" id="20300at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052014">
        <property type="entry name" value="Dictyostelium_Tiger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057594">
        <property type="entry name" value="TgrO1-like_Ig"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31341">
        <property type="entry name" value="IPT/TIG DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24612">
        <property type="entry name" value="Ig_TgrO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035149271" description="IPT/TIG domain-containing protein" evidence="4">
        <location>
            <begin position="23"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="782"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="domain" description="TgrO1-like immunoglobulin-like" evidence="6">
        <location>
            <begin position="107"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="5">
        <location>
            <begin position="270"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="5">
        <location>
            <begin position="611"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="5">
        <location>
            <begin position="689"/>
            <end position="768"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2070745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="817" mass="87297" checksum="49B9B682BD7DABF1" modified="2022-05-25" version="1">MKKLSSILLFLILCTSISFIHSLDIKSQNVTRFTFVQDKMESIQFLYNSTSVTLTPESCSPDCIFEFKNYVKLWDAQTFIYKYTSAGKVLTKSSPFDKFGYIESLAPPKTSGSNQTFTAYFLQINPTLVKSFSAMINGQSYENEDLNLALVNAGDYTKYSITFPPGTGKIDITYDKKTMSINFAAPTIASHTMDADGKFSVVGDNFFNNASVIAITVDGKALVDVNGAIVSADFQKLVFSYPTNTTSSHKIEVTVDGIKSAAPYDLVIKPIVTGISSVPNKGGKVTVTGKYLNAVKEDGTPSVVKVTVGTTECAKPVNPQEGVFTHLVCDMPAGNDDNLDVFVEIDSIKSDAATVKFSYGFPTISSIKQIGNLFVVTGSCLGEVSTSEVIVNGAAGIKSSAISNDETQLNVTLPASTVNGKLQVKISATKTSIASIFKITPIIKSISKAATRGSTIVIVGEFLDPTKASLKDKDNQGFNCTGFTQTANATKGTSVSCYAPPGVGANHTVILSIDGMTAPGVFSYIPPKIDTVEQDFGYGIIKGSNFGRGVSDVKITFFNTQVVPATVDDQDYIKFHIPETANPGSISISVGSQPSNILNFPLKTVISGHDPISTAGGNLAIRGNFFNATGVTTIKVGEIECTEPTVSDDRTLLSCNLPAGSGKNSNIVVSNQGVEVSNPNQILFSYAPPSINSVSEINKDGGVLTIDGYSFNNPLSVSVGQFICENPAVENYNRITCNLAKITDEYENDTEYDLVVGVNGSSASSKVTVKGVPSDKDNRIRWLVPAIVIPCFFGLVTIVLVTLFLVKRHKNKQGGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQB7</accession>
    <name>A0A8J4PQB7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_006993</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the retinoblastoma protein (RB) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000349">
        <property type="protein sequence ID" value="KAF2071693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQB7"/>
    <dbReference type="OrthoDB" id="844594at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000134">
        <property type="term" value="P:negative regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002720">
        <property type="entry name" value="RB_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002719">
        <property type="entry name" value="RB_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028309">
        <property type="entry name" value="RB_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024599">
        <property type="entry name" value="RB_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13742:SF17">
        <property type="entry name" value="RE32990P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13742">
        <property type="entry name" value="RETINOBLASTOMA-ASSOCIATED PROTEIN RB -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11934">
        <property type="entry name" value="DUF3452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01858">
        <property type="entry name" value="RB_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01857">
        <property type="entry name" value="RB_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01367">
        <property type="entry name" value="DUF3452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01368">
        <property type="entry name" value="RB_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Retinoblastoma-associated protein N-terminal" evidence="9">
        <location>
            <begin position="169"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Retinoblastoma-associated protein A-box" evidence="10">
        <location>
            <begin position="481"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="133"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="375"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="969"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="160"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="375"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="971"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1008"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1043"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1053"/>
            <end position="1062"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2071693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1062" mass="120899" checksum="37580AC8AA0FC414" modified="2022-05-25" version="1">MDINNNNNNNNNNSNIHTPPNESIISTPTSTTPPPPQPPISKNSSALSVLYGTMEELVAAADSLSTRLLDLSELKTLFDYITSILNVDNDTLDQAWSLIVNLQLPNDTTELQQKYYIASALFLSGNSRFSNINNSKSNEVDNSNNSGSSKKTKQSKKKQSSNNNNNNSSKLEIKNNNIKPHTIILSQLLKKLNIDILPFFENLKSFLSLSRFNSTVEESVKNLENSFNINSLICRKYEFIFYNLFIKENNNNNNNQNINLTNEEYLLNIGWLLYLFCKSQLFGDVPDLIQSTHLILCILNLLYIKSSQDYRKIAIHQIIDTDSHSIFQYLSKQTNCNHDDLLSFNKDTFILLLDQLISKDLFKFKFNNNVDQNDFNNINNSNNNNIDQDDDNININNDNENENNRDDNNLQDIFTEQLLKFNYTSLNKIYQHNYYINGEIDERTLIGSTTTPTTNVNSNNNNNNKLLSNRKYQQLNTPGGTPISAALSMESWMNHIIKNEKDPIPSDNLELLLKNLVEQKSIKPETKSNIVNRIETLTLKVNNLYHQDELAFKNENDLNLKRKNIAIRLYFNLLEKILLKEELSASPSFETLLSNEDFHKSVLSSSFEMVSYIYKLDRFYFPYFVNIFQIHPFSYVRLVEWIIRVEESLPKPLVHHFNFIEEKILDKYTWIGDDSKVFMILSNHKQILSTNPQQSPKVIYTFYKKLNNYINIRCRKMCQALNLPMDVVYQSCLVMSKLLLENNLLLRNRTIEILLICSLYGVCKVNHITISFKTIIEKVHIPQKVYKDIVLFDDETNTGANEYGDIICFYNKIFLKKMDIIIHSISQNFKKQNNSSNTTPTTTTTTTTILSSTSNLNNFSTQSPIQNKQINKVLNHTPTKNVIPLSPQKNLNSNFSISISNNIISPMKPSTPKSNNNINFNNNRDITTITTGVNSPIFGTPSKNLLTFNIGKSPAKELQQMNQNMSIFSQTPDKSTTSSIKKTPSSLTTTTTTSGKGKRLLFEDDDSQSSQSSQSSSSSSSSSSPPSQLSSLSQSPNSQSPPTHTPSPPSSPSPTSSKRLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQJ1</accession>
    <name>A0A8J4PQJ1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006749</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000325">
        <property type="protein sequence ID" value="KAF2071934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQJ1"/>
    <dbReference type="OrthoDB" id="21179at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007175">
        <property type="entry name" value="Rpr2/Snm1/Rpp21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742:SF4">
        <property type="entry name" value="DDE TNP4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04032">
        <property type="entry name" value="Rpr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="233"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="332" mass="37453" checksum="452C9F2801F361A1" modified="2022-05-25" version="1">MSFDYEQHLKKTTKNKSSQIKNNNKNSNNSNNNNSTNSSLLGKRKQSYNAIQYKDEEDINETVKNHHNQREIQFANHTSYQDQESLEQEHNTKSQLNYLWSLSHYSQPGNPNLGRYFMYKFREMANVEHMRGIPPMVESKNCRSCGQFLIPSVNCRVRVVSKSDLSNKSKSMITTVSQEINSLQYKTKKAQNFKHVNAVVKTCLFCSNLTPIRAELKEKKLERKANVKKFKASLSNNTNTTNTTTTTTNNSTTSPTLASSPTTTSPTLQSSNKNNNPSNKNKSNNNSTSTSTSNSNNSSNNKKQVAKAGSKNIKTKDDSLKDFLSNMGSLMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQL0</accession>
    <name>A0A8J4PQL0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_006882</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000337">
        <property type="protein sequence ID" value="KAF2071798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQL0"/>
    <dbReference type="OrthoDB" id="19756at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008278">
        <property type="term" value="C:cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039662">
        <property type="entry name" value="Cohesin_Scc3/SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056396">
        <property type="entry name" value="HEAT_SCC3-SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020839">
        <property type="entry name" value="SCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013721">
        <property type="entry name" value="STAG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199:SF0">
        <property type="entry name" value="LD34181P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199">
        <property type="entry name" value="STROMAL ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24571">
        <property type="entry name" value="HEAT_SCC3-SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21581">
        <property type="entry name" value="SCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08514">
        <property type="entry name" value="STAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51425">
        <property type="entry name" value="SCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCD" evidence="3">
        <location>
            <begin position="230"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="505"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="867"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1138"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="195"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="513"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="902"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1141"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1161"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1175"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1189"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1216"/>
            <end position="1227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2071798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1252" mass="143316" checksum="D9C1AEFDD9EA4E9E" modified="2022-05-25" version="1">MPPRKKVTKTTGDTAISKKRQITRKKKVNTSDLFEKIKKKCNVTDLLDDWIRFYSEDPERALYDLLMMVFECSGMEGVIDYKSFVELGIHDGASEMLKLKDQPMFPLGTKQNKNILANYKQFWSVIIIKCAESILFDKYFLEMMEQWFKELKSAHKRGTRYATAVACIQITESLISVANDIKKRLGLANRQYTSETKNTSRSKQLKENLEQLETRVKIIEAHIGKMFGVFDTLFKDVLPELRSICLMPLAHCILAYPGTLFADKYLEPIRRALNDWMTEPRQTAVQALITLYSNENISRLDSFTSRAKHRIVEIAFCDKVPAICIQAIHLVALMNQHELLDDDDINKICRLYLVDQKDISQAAGQLIYQKYFQDTEELVADATAKKKAGDRFKHRETQFNTLIEFVETSPAPNIPYYAVQALWPHASAVLGDWEFYVKYFEDIDSKDLSDRQLALFCQILNAAVRLAVGDTYDTFSLENRQPLHLAVTASKKFGHKHVGKKVLNEKSTAITTDNEEERVETDEEAQEPEQQQDQPEEKVIDQKVAGIVTNHFISLLPALLVKYRSNAIACENLIEICKYFTLDTYLSSRHQTKYNELLEIISSIFLLHSDSSLIKTTACTLEALFHKPDIPSQLDAISKQSLLQLYNSVMDALKATLATHKLSSKKKGDDDIVSSIETSQQQQEKDQEAIVFSVISNLDKLNQLAKQFYFEQEEFEQMVFSFTQGQINDQDDIESSVSRDKVISLSLEAYTTTLMWCLFNANPADQLLHTTDSKILRMFYQFCTVALSLLRSDDLSHSLRHYIVRNLVEIRSMFSLAYRETLMHAYSISMPRNNDLLEKNIELLFSHEEAYALDSLKVEFLKSQKETQEKSTLQKQNRKKKAAEKKAKSASAATTTDDEAGKTTTAAESTTGASSSDMDTSDKETTNDEADKEAAEQIDQDFIDRFEFDPLYEDRVEELVLSTCFAVRRLIVSPKTVWRLIEMIPLAPNRSIMDIVKTFLSEITMPTVEEEATVVKSIVKKFHSICTSDSIASSSNFQLNMFSRLKYLIQWMSSDFIQHPKSTGLAINDILQEIKLHNSFAKPEELLEILNFVASKSNEQATLYLIKNRIQFDDDYEFIELIEKLYNTIELQTIAEDTKTMKKSSKGKGKAKGKAKVGDINNNNNNISKSTPSSQRKRKRRTKKKIESSSEEDEPSESSEYSDEQDEIESDDDDNNNNNNNQKQQQQSEEEIEENSENSSDFEPSQPKSRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRC8</accession>
    <name>A0A8J4PRC8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 2 profile 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_006448</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000297">
        <property type="protein sequence ID" value="KAF2072248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRC8"/>
    <dbReference type="OrthoDB" id="100006at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15040">
        <property type="entry name" value="7tmB2_Adhesion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022343">
        <property type="entry name" value="GCR1-cAMP_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022340">
        <property type="entry name" value="GPCR_GCR1_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053231">
        <property type="entry name" value="GPCR_LN-TM7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45902">
        <property type="entry name" value="LATROPHILIN RECEPTOR-LIKE PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45902:SF1">
        <property type="entry name" value="LATROPHILIN RECEPTOR-LIKE PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02001">
        <property type="entry name" value="GCR1CAMPR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02000">
        <property type="entry name" value="GCR1PLANT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="148"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="225"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="10">
        <location>
            <begin position="9"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="278"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="278"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2072248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="310" mass="35805" checksum="E7AEFDA44758A64C" modified="2022-05-25" version="1">MNQTVNDVLSYIVDVLLALSIVGSFCTVLTFMLFVKLRTYPIRLILYLCMAIFFAQFFFFISFKAYDTLFCLPSGLLLHYFFLADFIWTFCVAFNFYQMIVKRNRDAESFEKYYHMAAWGLPMIVIVFCAGFGKYQNRGGFCYLENELPVFLGFFVPGLLVVCANAVIFFFIAREIHDTLKNAPKTSDRKEKSKEFRVYFSIFVSIGLSWIFGFIMTFFNDDVFSTIFLVLFSVTTPLQGALIFISYCVNEKVFSHYIGLVSRVFPYCKRFETNTVGNTSTTQNSSTNTHTSSRSENLSSDSEISMKEYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRR5</accession>
    <name>A0A8J4PRR5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_006908</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000339">
        <property type="protein sequence ID" value="KAF2071780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRR5"/>
    <dbReference type="OrthoDB" id="765884at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45188:SF2">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45188">
        <property type="entry name" value="DNAJ PROTEIN P58IPK HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="2"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="73"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="189"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="273"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="307"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="6">
        <location>
            <begin position="361"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="498"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="333"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2071780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="521" mass="58333" checksum="CF00550DAA60A65D" modified="2022-05-25" version="1">MAEALKNQGNDYFKQQLYNDAINCYSKAIEMDSAVPSYYGNRAAAHLAIGTKSSLKEAIEDSSKAVELDKSFIKGYTRAAKAYVQLGQYDSAQRVLVQGLVQDPRNTELLTEKTNTEKTINMIKQLLDSPDLCTSNPQSSLNSIENLISTAKYDNRLQVLRCKILIELKQYAKASNFMTTLLQEDSRNPEYLYIRGLALYYQNSLTTALQHFQNSLTYDPDYSPSRIALKRLRLVENKKKEGNEAFSSKNYPLANQLFTEALEVDPKFDVLNSQLYSNRAATFVQMNKLSEAIQDCSKALELDPNYVKAYIRRAQCYLKTERFEDAVRDFEKAQTLDSENEDLARQLKEAKVALKKSLKKDYYKILGVSKEANDNEIKKAYRKLALQYHPDKNSTLPEEEKAHAEKMFKDIGEAYGVLSDEKKKRMYDNGQDENGMPFDASEMDFSNIFNMFNGGGMGGGFHGGHSHGGFPGGFGGMGGGGFGGMGGGFGGFGGMGGDFGGFQQQQQQGRSGRKKTEFRWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS05</accession>
    <name>A0A8J4PS05_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006017</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000258">
        <property type="protein sequence ID" value="KAF2072683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS05"/>
    <dbReference type="OrthoDB" id="19328at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2072683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="164" mass="18860" checksum="D51D1B64E32B5881" modified="2022-05-25" version="1">MMINNTFKLIKNTRCITNVLNSSTGCTLNNNRYYSTEGESTTGEEQPDVATIQDVNELINRRPLSAKKIKQFYSIKPEAGSIKPQDMDIINTIRTNKTKIVAMPFDFSLDAFYKNKRATKTVTRRKKHLNDFGAKPNIFPESMFRDYYMKPIPYSVVTQGNQDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS08</accession>
    <name>A0A8J4PS08_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Signal sequence receptor subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_005277</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000206">
        <property type="protein sequence ID" value="KAF2073398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS08"/>
    <dbReference type="OrthoDB" id="1926781at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005595">
        <property type="entry name" value="TRAP_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12924:SF0">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12924">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03896">
        <property type="entry name" value="TRAP_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035267105" description="Signal sequence receptor subunit alpha" evidence="9">
        <location>
            <begin position="21"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="191"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2073398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="26722" checksum="07B8ED029C5E53F2" modified="2022-05-25" version="1">MYKYIALLLSVLLILNSVYSEEVTVTEDLENTVGQEVPSDVSFSYIFPDYQDKHFPAGSVIEVLVGFSNNGQETKNITHIFASLNHPQDFSYYIQNYTRGEFGIAVKPGHHATLAYRFVPNELLDPRQFGLLISMDYQDSIQNYTHTFFNSTINITEKDSTFDLEQFFLIIFGLGIIGLVGFLVYNRMPKSKKSSRSSKGAQVVFNEDDQSEWLAGTSADSPKVTPKSPKTSKPKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSC8</accession>
    <name>A0A8J4PSC8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000193">
        <property type="protein sequence ID" value="KAF2073623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSC8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="215" mass="24576" checksum="E04ED7E6441E5867" modified="2022-05-25" version="1">MNYQPATTSINYKKVGYFKQRRGDIKNNSIATVCCIDTKSSLNNYYNNNNNKSSIRVLRHYTYSKRNNNTKPNNLLILLSKRKRVVLHKKIIKKNQQKKTNTPIAISQQALNFNTATVPNTTAEMKPESLNSPYNSPMFSKDISRDFNSTCPGYAPFNSTEYLTQNYYDILHLEDPQHFATAAVDQQLNNYGSMFDLIKDGNNNSLFPAQIVPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSU3</accession>
    <name>A0A8J4PSU3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_005551</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000224">
        <property type="protein sequence ID" value="KAF2073135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSU3"/>
    <dbReference type="OrthoDB" id="419616at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17330">
        <property type="entry name" value="MFS_SLC46_TetA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF4">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="415"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="468"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="500"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="533"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="571"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="610"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="92"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2073135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="667" mass="75231" checksum="0B292029D4D39522" modified="2022-05-25" version="1">MEINNKELNSSVDSTNEKDNSIILDSISSTIEEHVRDEIPLFSNTPSSLDSHIIDEINIHNSNININDTAEEIHIQDGPIEPIKKTPLPKMKLFSIFITLFCDAINSLSLFPYMNFMIEDFHLTNNEKELGYYVGVLASSYYIAQLFSSFFWGWFSNLKGRRPCLLLGLIGSMVCLLFFGFSKYYFLAIIFRFLCGLVNGNVAVAKTMLGEITDSTNQAKAFSFIGLSWSVGGIIAPLIGGLTSNVCTHYPNTFNDPNSFLCKHPYILPNFICILFSATGLVLNYFYLQESKTFTIKNKYSKIGSKSNLNLTQLESTNNSKLNQLKKKFNSITNRIFGTPDIYMIQLDDVDTKEKKSVHILEENNQDLYDYDGADVINIKKSTNSLKKTGELNIEEEDDEENNPRPLKDLYKDKIVIYACVSYAFLGLMFTMFDEVFPVWSPIPRIRDPETGQVSGGGFNFESRQIGILQSCAGAFALVIQLTVFPNVVAFLGLLKTYRFALLMVLPTWLLLPELSRFVILDPSEIHTHSYPAIFWVLLFPAYLCQSFASELTFIAIIVMISNSALPKDMALVNSIAQFLVSISRSIGPIMGSSILAFSVSHNLHYPLDFRLVFILMACLCISLFFASFVLPKSLDHTKDQEIKHQIKLSMSKSENLSQLETQPTLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSW6</accession>
    <name>A0A8J4PSW6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">TalinB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_006287</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000284">
        <property type="protein sequence ID" value="KAF2072395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10262320at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10569">
        <property type="entry name" value="FERM_C_Talin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17089">
        <property type="entry name" value="FERM_F0_TLN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17090">
        <property type="entry name" value="FERM_F1_TLN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000007">
        <property type="entry name" value="Talin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000028">
        <property type="entry name" value="Talin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.230">
        <property type="entry name" value="Alpha-catenin/vinculin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1410.10">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.10">
        <property type="entry name" value="Talin, central domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036723">
        <property type="entry name" value="Alpha-catenin/vinculin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032425">
        <property type="entry name" value="FERM_f0"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035964">
        <property type="entry name" value="I/LWEQ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002558">
        <property type="entry name" value="ILWEQ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049108">
        <property type="entry name" value="Talin_R4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054060">
        <property type="entry name" value="TLN1-like_RS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19981:SF1">
        <property type="entry name" value="RHEA, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19981">
        <property type="entry name" value="TALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16511">
        <property type="entry name" value="FERM_f0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01608">
        <property type="entry name" value="I_LWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21692">
        <property type="entry name" value="Talin_R4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21865">
        <property type="entry name" value="TLN1-like_RS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00307">
        <property type="entry name" value="ILWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01244">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47220">
        <property type="entry name" value="alpha-catenin/vinculin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109885">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00661">
        <property type="entry name" value="FERM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50945">
        <property type="entry name" value="I_LWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="7">
        <location>
            <begin position="85"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="I/LWEQ" evidence="8">
        <location>
            <begin position="2251"/>
            <end position="2491"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="2458"/>
            <end position="2487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2072395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2491" mass="268214" checksum="ABE56A4001101F13" modified="2022-05-25" version="1">MSIMLKINMVGMGTVKTLRFSPDMSIQEVCTHIFEKTNEGGADHGLYQPNIEGKQTAKWLAMGKTLAYYDINSDSPPLDYKKKHRPQKIKLLDETIKTQLVDESTSVSDIIAGIAKKMGIKNPEEYSFMNSKGDWLNNNQILSEQGVTEADIVVMKKKFFFNDANIDRNDPVQLHLLYVQCRDAIIEGKYPAQKEELLSLASLQCQVAMGDYSPAKHVSGYLNIKEYLPLQYVKTKNIEKDIYKEYKKLVNMSEVNAKYRYVQLCRSLKTYGMTSFEVKLRVPMKKKMVDHILGITREQMILIESETKEVIKSHPLKHIKRWAATEKSFTLDFGDHEEEYLILFTNKPDEISQLIGGYIEIIMKARRDTSKTITTNDESVGVEEVMAIKKGNVATSSTFMGYGGGNGGGHQLAPSQQIAITDLKSALRATDLLIGELNTFKGGNTATPQTFTRSFTTLTPQQFKHQLITHTNAIASAANMLFNDMSNPPAQGGIAAAQQSILKRAQIIMAELNTVGTSAKNAAYFPDLASFSDEIINCATKLSECMSKLLGTASTIQGDRVDEKGRLLAQNEIFNVNSLASMMIAVCDNMYVTESSSNLIIECAKNVSAALALMLSAGSEKINLIDDELLFSQIESTIKTTSLNNEEMLSTTETLASTSCHPESRKKITDITQAAVSNANSLLTAFKSGEIPEEDYAMLSSRVQDIMESVSLISYALDCAEQEQKISITSNGTEVGQGELLRGTNLSEEFSAVANDLTASLMTLRTNIKNPDDILESYRVIANNANRLISCTKAVASRADAQSQQRLFNSANAVFESVAVLSGHCRAYVKNPENESFQTSIMESAGHLQFLTQNMSADAGKIATIIALRDNSKDMISHVSSLMSVSRSSAQFLPDQSGISLTKASKDVTDALARLMIGIKKVVADPKSESTQMELLTSAQKQSLAPMNLVSTSKRVAPKITDPNQKQALMFASDAASQSVQRLMKSCEAYKKICGHIEIEEALEAFESTISDLETTEIAIQGGFLDTVPSQAREGCTELLMVSIKELNNAKEELLTEIKVNPGRLGELVKNTTSSAGQVALASKSLICATPGKQAQKKLAGITKQLMNDMEQLIRASRSVASNPDDPASELLLDAATVDVSTSVASLVGAASHIDCKELDEASTEITALIAVKLNSIDGIQVQPTEDLSYYSEELVSSTKALNAAILQVVAMARAKNLKGLGVSAKITSSTLTSLTNNAINAIALSENEATKQNILSSTAALGQQIIGVLDFAKARVANYKDPIYDQNLLQCSKSVEDHTNKVIRALGSTGSNQDCDEAIDRIIEVSRLLDQTFMPDVSGTQTNSALEMNHQQALLTMTESSKKLGSITSNLVSSKNNGEILGKNSMEAAESVAALVEAAKQVITCTISTSPPDILLPTKAILDSVPMIASNHNNLAEIIPYARTIATNTSALLNITRERTAGLREDDERQQLIKESVVKATQQLAYSTSNLAKAVKALTAQDPNSQSLISQALKDLQTSSTALLVSTSVPSIDRGISSADFEKLLATTRNVCTSSTNLITSASSSSSKPKDQELQSILSESAVEMTSAIKDILKVSSSMMPGVNFCEEAIEVAQRSIGDLSSMALSVAVGSFDVPSNNKENLSMVESQERLAEITKSIGAGINDLLKASRQSPEAIGVSAKSLSFIAPSLVSATKVALCTLSEQDAQNDLVTESKNLGNSILKLCNASLQASSNPSKETYQAIVTKCSEASEVMSKLVAQISSGVNLIKDLEECIEKIKKSIISTNAKENQDSKSYQEYKEDITADTKHLAIALKSIVSTDQSNLVQISTMSKDVANYISNVAQNVSSILFTTSDQKIRDSIVINARQVIQNTAEMVALLKSGAMDKNSSNQTQINDAFRSTHDGITRFLQSLKQGAIGEILSDAAVENIRKVITDLDAYSLFAAAGQLDEDSLSSSKLDQATQQANLKNLQKDIVYGSKLLIVSASQLVGSSKGTQEHLGSATTKVANTVTDLVKSAKEIASVLSDIGSQQDILSASKALSIASQQLVLAGKDAQRFKKDTTAFRSLGKAAEGVAEVVGNFLASVYHAISEAGRGIKELEKTFVLVTSYHEKPENIISNSKASAETFAQAARDVAKSSIDIVTSYQTSQDELVKSSQELSASVQSFISNSKGVLSLLGTTAEEEELKTKVNENVKNVTLTVLNHIKQVKDQDKGVTGVKVSASSRAVADNIQSILAISKSLPGGQNIVVDDDITEDLEQTAEDELLACAKSIEEATANLIAARPKTKITKGGMLDAEGIAATIVDASSAIAQAVSRLVHAAAVAQGKRRENAVGNFYSKDPTWSNGLISAAKAVAAATHRLVEAGIKAGTGKAEEEELIATARAVAAATALLVSASRAKSGDDSGSQAAHSALSAAAKKVAAATSDLVSAAKAATVFEEQQEEEQQQDFNFTGSKVKELEQQMKILRLEKELESERRKMLNSRKQNYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT83</accession>
    <name>A0A8J4PT83_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_006087</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000266">
        <property type="protein sequence ID" value="KAF2072590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT83"/>
    <dbReference type="OrthoDB" id="266020at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009967">
        <property type="term" value="P:positive regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000383">
        <property type="entry name" value="Sex lethal, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050886">
        <property type="entry name" value="RNA-binding_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48024">
        <property type="entry name" value="GEO13361P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48024:SF56">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN A0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="15"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="120"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="411"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="234"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="260"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2072590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="492" mass="54644" checksum="519F0FCE92196EDA" modified="2022-05-25" version="1">MEMEPIAQDQDVDICNVFVKYLPCDFTEKELHELFQPFGDIVNTKVMVNIKTGNSLGYGFVRFSQPTFATEAIKNMNRYQVGYKTLLCKLSKPSTSPPLQSNIQTTVTPAADEPEQTPSNTLYIRVLSPTITDPILKSTFSNFGDVIEAQVLIDNASGKSKRAGVVKFSNIESAMYALQCMSGSLTLGETPLVVRYATSSSSSSNSLSASNSPSLSSANSPKYIKSIPSPFILGQSQPPPLSLSTGSIKLSRSPSPPPINQLSQSQQLQQQSQPQQILIPQHYTYVYSNEQYQNYYPQDYNYSPTNYIHPQQQHYHNTYMSGSPSGSPPSNSQAPIYNGMPYHTTTNNNSWTQGHESFYVYPSTTYYYHHHHHPSSGGIVSNQQNPIVYIPTSPTGSPQISPRGSKSIDFSSILICTFKGNLEYSNLIQVFSKYGDLKSINININPNNGNKSKCFISFNNIENAILAQQNLDKSKIGNIFVRVKFLNKPIHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTR2</accession>
    <name>A0A8J4PTR2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004977</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000187">
        <property type="protein sequence ID" value="KAF2073718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTR2"/>
    <dbReference type="OrthoDB" id="10690484at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="438"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="965" mass="113414" checksum="0CF2F94A746E4E04" modified="2022-05-25" version="1">MNEVLNNFIQSIQEENTLALNDNIWLIVRNVKSIFLTSQVKKWKESGKFYNTIDPVLKYLLIGDDFFSKNRFLKELVFGYNEIVSIFGQSEQVKKFDILFQIWNLMTFKITLGVHDGQSTSLFTKDSFDIIFEFEKSSSSDKVYQSHLYLLGYPEVDCFDSVNQKYIYKAFLHMDLLKSLLSETAFQKDQIDFIISTLNIIEKLMNLSINTNSWKEFDGDEIKEIEIHFKINNLNDILSSSTSLIESFSLKEIFCCEIYHQLLLYLNQLINLNLQKDIPTDEKTVIISLFFINSLETEDGKNDFQEIWKEKSKFWALEKFHRFYNYTFTMKEPQCDILNEKLLCHQHNITLLNNPYKTKDEIMQEEEQVSFVEKEQKRKEKDKKKKDQDYDSPYKDYISSERKYEDTYKPTLQKEKKSKEKIEKFEKVKNTKGEKTLKVYERREEDHVSKTPRKEKKSKEKIKKIKKEDNSPIKHINLNEGSDVFSPIQLETNEKDKKDKKKYHGASYNPCQGRDEEFSPNKPYNPSEEINVPIFDIKLDSSNQSLSDPSINQKKIASQIASSDCTEEIFYEPPPYVEVECYSTSEPSIYQGQEIKIEMGTLSFRHSSFEDYSKCLNDSETLNFFRDNSILENDTMSKEIVYFMYYNRNDKLNISKSIIDQFLREFSQLTMTQFDPVHYNQILTCLETLYLKPLNCAKFNPLIEYLANSNLVSCIAQIFAFKFNLSNDESWEKVFLQYSQLIIHLVEYLSFKDNIKSHYTSIKILLPVIDLSIFIAPPNNQFYISLANFVINIFNPKFNSFNSKIIKSFKSSKIIESLLFHLYSSDNAESSVQVLNILIFIYKLDKKIRKVISEKAKSAVEQDLYFAGQLTAFLEKSISSVLDRNNIKSFVCNIINPVLQDSCSDSEEFTEDDGDYGGEVYDHEDLYVSEYGTENQDCFLEEADYDQYESEIYQDDHLYQNSETY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUA0</accession>
    <name>A0A8J4PUA0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_004669</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000169">
        <property type="protein sequence ID" value="KAF2074018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUA0"/>
    <dbReference type="OrthoDB" id="267397at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025390">
        <property type="entry name" value="Dsc3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13373">
        <property type="entry name" value="Dsc3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="3">
        <location>
            <begin position="42"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="125"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2074018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="272" mass="30805" checksum="1816B8978786E1BD" modified="2022-05-25" version="1">MKSTHYKKIDSEDTESPTYEKNNGSSINSNSNSNKEDDYNSNEVVVKIKNGEDPVFTLTMKQEDTVQKLKHSILAHKKLEFDYHVRMILNGRMLQDHLTVSQSKILNNSVIICMITDPLPTISKPTTAVIPPPTTTNTSTSIPPPSTDPDPESLNQQAILEILREEGREDLWQNSIQQQQQQQQQNEQQQNEHQFYNNTNNGFEMIPVAAARDSIELLVGMIFGFVFGPLALFWVAREYIPRNTKLGIFMGIVFGLLLSIIKLTRANNMILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUK3</accession>
    <name>A0A8J4PUK3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005322</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000208">
        <property type="protein sequence ID" value="KAF2073377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUK3"/>
    <dbReference type="OrthoDB" id="10261348at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013169">
        <property type="entry name" value="mRNA_splic_Cwf18-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31551:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31551">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWF18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08315">
        <property type="entry name" value="cwf18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="166"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="311" mass="36008" checksum="58F80878D20AFFC9" modified="2022-05-25" version="1">MTGIDWNAIESLKRNELQKLCKELKIEIKGTSKNADLIDALLAYKKENIDTSNQVTTDHNDNNNNDTNIKEKEEPVITNITKDSTTSTPKKSNKRKTKDVEMVEETTKEEVENKETIKEKDTIEKTNVNDKTTIIEKSSNEKQQKEKEETQQTPKSNVSIEDNDQTKTTELTTTTTTIDNETMDQDQDGNDDHHVEIQLEEEETLVGLKFRNYQPRDHKLNKYRVEKTKAPPIVDELLARLQLLETNQEIQISFAPKKINWDLKRDSLKKITKLDKLTEKAIYQLIKQKIESSNDEESKAYLLSKNIDKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUP8</accession>
    <name>A0A8J4PUP8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PABS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_004879</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000181">
        <property type="protein sequence ID" value="KAF2073821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUP8"/>
    <dbReference type="OrthoDB" id="38125at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.140.10:FF:000001">
        <property type="entry name" value="SPE3p Spermidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000013">
        <property type="entry name" value="Spermidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="entry name" value="Spermidine synthase, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030373">
        <property type="entry name" value="PABS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030668">
        <property type="entry name" value="Spermi_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037959">
        <property type="entry name" value="MFS_SpdSyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002010">
        <property type="entry name" value="PRK00811.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00417">
        <property type="entry name" value="speE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558:SF11">
        <property type="entry name" value="SPERMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558">
        <property type="entry name" value="SPERMIDINE/SPERMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000502">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01330">
        <property type="entry name" value="PABS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="5">
        <location>
            <begin position="6"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2073821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="284" mass="31594" checksum="6AF6D0DAA1DAE6CB" modified="2022-05-25" version="1">MDQIKNGWFSEISEFWPGNSFSLQVEQVLHHEKSEYQDILVFKSKTFGNVLVLDGVIQATERDEFAYQEMITHLPLYAHPNPKRVLVVGGGDGGVLREVVKHSAVESVTLCEIDKGVIEASRKYLPNMSVGFDHPKVTLFIGDGLEFMRKRKGEFDVIITDSSDPIGPAEGLFGRSYYELLLQALAPGGIVCSQAESMWLHLDTIATITKFCKDIFPVVEYAYTSIPSYPGGSIGFVICSTANSCKSPARAVPQDELEKMQYYNENIHKASFVLPQFAAKSLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUW1</accession>
    <name>A0A8J4PUW1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004606</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000166">
        <property type="protein sequence ID" value="KAF2074077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUW1"/>
    <dbReference type="OrthoDB" id="20747at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.980.20:FF:000015">
        <property type="entry name" value="Histidine kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.980.20">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="415"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="248"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="415"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="469" mass="51995" checksum="29A0F1BAB1755702" modified="2022-05-25" version="1">MKDSIIIPTVNLANLNKSPSTSNPCSPHTKFVSTPYEYDGCMLCVNGTPSCLQKRRATWTLKLRVALYCLSVLHPEKKYFNLKKDIYTYVDSHWDHLSINTAKSDRWRKQVQDSLSHCRHFESGWDAMGCNGHWRLIQPLNPWSLPPDKENSIYEDEELLCMTPESGLSPRTTKSPREDPLYRNFPKRRPMTMSGSNIATVSSLNDDEIENRKRKASDEERYTEEELVLLKYSPMIYSDTPSPKCRTPRSNNSSTTSPSILGNSPLLTSNGTHFSLANFNNNLTLNSPSSNNNNSLLNPNNSGNSIPTNITILEQNTNINNSPPQSISPSLQSTGNSSSPPPIPSNPFSAATWKTTTKQTTLFSTEECFKVSPLPNPFSSMAAKSNLPGFSISNNSTLKVPSELLKSSINNSIHLTNSNNNHSGNSSPVNIVNGNNVSSSQQDSQVGTPTQSSNLTKLQQQNLILNCTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUW7</accession>
    <name>A0A8J4PUW7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">40S ribosomal protein S23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_008618</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000549">
        <property type="protein sequence ID" value="KAF2070061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUW7"/>
    <dbReference type="OrthoDB" id="1713912at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03367">
        <property type="entry name" value="Ribosomal_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000007">
        <property type="entry name" value="40S ribosomal protein S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_uS12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005680">
        <property type="entry name" value="Ribosomal_uS12_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00982">
        <property type="entry name" value="uS12_E_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002133">
        <property type="entry name" value="Ribosomal_S12/S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2070061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="15617" checksum="D44EA5BCA89A0FC6" modified="2022-05-25" version="1">MGKPNGLKTARKLRNHRRTQRWNDKLYKKAHFGTALKANPFGGASHASGIVVNRLGIEAKQPNSAIRKCVRVQLKKNGKKITAFVPNDGCLNYIAENDKVLVAGFGRSGHAVGDIPGVRFKVTKVSSVSLIAIYRGIKDKPNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PV86</accession>
    <name>A0A8J4PV86_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">cAMP-dependent protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_004554</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000163">
        <property type="protein sequence ID" value="KAF2074151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PV86"/>
    <dbReference type="OrthoDB" id="20309at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05580">
        <property type="entry name" value="STKc_PKA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF87">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="589"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="9">
        <location>
            <begin position="845"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="99"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="306"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="397"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="8"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="502"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="99"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="127"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="159"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="306"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="340"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="404"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="479"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2074151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="909" mass="103732" checksum="EA134F075FDB361C" modified="2022-05-25" version="1">MKNDHFLALQQQQMNHQQQQQQQQLQQQIQEQHLQQQHQQSILQQQHQQQQQQQQQQQQQQMQQQNIQLQQLQIQQIQSALPHQQMYTKTTEQQQFQYSTDPNNNIHNIKSKASKKRSHEETLDKPNFQYNKNNNNNGSHNNSGDHSFLDSSSGGGSGSSSSSSSGSGSSGSSSNMNIETKKHITESNIINNNNINNESANNNNSTTGSSSSSSQPIVVNSLILRSIGMMTSKISKDFQELITFMQSSCPMLLQYSGIQNSHLIVDKLNSIINQSLEGSKQQKPSSSQPPISQKLVLPRQIQETLSSTQPPLQQPPSLTMNSTLQNPFSTPPCSSPPILNNINSNNNSPSHSNNTTASNNTTTTTTTTTGLINPFNQIESFYSNYYQQRNSLLQKSPQTYQPISTSSSNDDSVNNNNNSSNNNNNNNNSRNIYSSPNNYSTTSSSSSGSSSSNYNINNIISNHDDNLHHRHMNPTYHNQQQQQQHIYQQHPQLQHQYIYQQHLQLQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQLQLQQQLQQEGNNNHLARISAQQFIIQNITNQQVQSTPIDFSNSSISDFKLLDLLGSGSFAKVRLCQHLKSGRLFCMKILNQNKIIRLRQEIHVCNEKEVLLQVENSFIVKLYTTFKDNKYLYFLQEFIAGGELFDYIRTHGNLSAQVTQIYAAEIILALEYLHNLDIIYRDLKPENLLIDHFGHIKLTDFGFAKKIKENTKSMCGTPEYIAPEILSGHGHGKSADWWSLGILIYEMLVGVPPFVSEGSQNDIFRLIREGRIIVPPEVDPSAKDLIEKLVVVDVEKRLGNLEGGIEDIKNHSFFSGVDWIKIQNREIAPLKPRIKPLKNLCNPSNTLDESMEDEDDRISTNFIKPEILEKRKKEFFSNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVI7</accession>
    <name>A0A8J4PVI7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003877</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000128">
        <property type="protein sequence ID" value="KAF2074813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVI7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF5">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035306215" evidence="1">
        <location>
            <begin position="23"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="3">
        <location>
            <begin position="632"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="2">
        <location>
            <begin position="772"/>
            <end position="889"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2074813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1059" mass="118076" checksum="CB6AD3E33F9B82D2" modified="2022-05-25" version="1">MKNYCLLLLLPFLLLLVQPSASVTLSISSRTPYLNHYAFGTTCQYNYTVGLLDLKGVNIISPSTITIQSVTQTRTSIVAIISYTVPTGTSGNLVLKTTNNDTTPFDFTLDAYECLAAPFIEYETVSWSKNPSSFGIYIKVKTPLTKYLYGQLFPATITSTQYFLYYNMADYQIYGFNFLILSPVTGILNQFNLVVKVSATQTLTIQITPPFISLPYLGHHYTETSTENSYYSMSFLKYENNLNNNTFLLKTSNQGGYMNNIVEGNPTKGTLMTMYNRRGLFVAAAAVREECYSLSSTSVVVFDKIKNILAATKITPSTYSSTYTVSNNRPSIVHFNAIYSVPTTKIYDRTHSISLTKVNILANQYLTFPYGISTGSLSSGFRVSLSYPSWAYFNNTMSYTVGMSNPIQSLTRTSPNYNPPNPTPTVKSLTYIGQFNGRYSYRISINDTIIFNKITAFYTGVDSTDQILATSANLVSGDGYDGTYEFSHDISGLKNARVINDNGYYAMITFPTTTPVTTDYSAIKGISFSTKFIDTTHLVEPVKVRVSFDYAIPNINFKFIMYTPGLYITQTSDQVYYDMVFNPESQLYEVDIDVLPRRTFTYYISSYSFMVYSMALEVMFPESVLTVVYDNLDEMPPMVSSVTHSPSSSITIDSAVPGYTTANFIITIKDTYNGLKNGTIWIASDLDPVGFNFTIDPSMATSGDMYLGTYSLPIKINRNCVSQSYFIQDMKLFDNSNIISSVYAPYPNPFFNESINAPLAITCSGTRETVAPILTSFVINTAIPLDVSAEIRNVVIDFTVTDTDSGIDEFRTPTVYASEPGFRYVSSKSVFISTGVYRATVSLPYGFGAFGGVYFSIYGIIDNHLNFNGYSTADLQSLGFANSINTTLNFTPFITNASFSVEYNLLTVKGFSFGSDGIIEYTTNLNPDAVFYQNTQTQFRSSNLIAVRRSFNTEPFKIRINKNGVISNTIIVEPVLPWLPNVLTNEAVQRMLLILQLIEQPLIIQPNKLAHILQLVTSLETNADIPQLETILQILKSRPTPQLQAILQLRQIIIQNLGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWE2</accession>
    <name>A0A8J4PWE2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_007932</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000450">
        <property type="protein sequence ID" value="KAF2070751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWE2"/>
    <dbReference type="OrthoDB" id="5337378at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF190">
        <property type="entry name" value="MITOSIS INHIBITOR PROTEIN KINASE MIK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="2"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="303"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="317"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2070751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="340" mass="39064" checksum="922412D97C6D97AB" modified="2022-05-25" version="1">MAQGEPRIGGSAFDFIFENRIDQDQDYNGSVWRAVSIIDGNAYAIKVLDFTRDRFEYLTALKTFDHPNIVKIHDVWHQFHRIYVQMELCLRSLNHVVSYNFVNRILSSPQQIFKIMSDVSAGLQVLHNCGIYNVDLCNKHLLIAPDGTVKICGFGVSSTHHTPSPIIFDFKTVTPEIMNNSPIFPLVYNPATDVYDLGLALYELYTCQQFLSCDKTWYRLRHDKIDFAYPQAFKPLALLLKRMLTSKPEARISIAEIMQSEYYEKGEPNPVLLSVIPNLPALNVMIYTRHSLAHALPHTRHYTPEPSIIPDPLPRPNNNNNNNNDNNNNRRSARRTINFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWM4</accession>
    <name>A0A8J4PWM4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Methyltransferase small domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002408</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000066">
        <property type="protein sequence ID" value="KAF2076293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWM4"/>
    <dbReference type="OrthoDB" id="269872at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050210">
        <property type="entry name" value="tRNA_Adenine-N(6)_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47739">
        <property type="entry name" value="TRNA1(VAL) (ADENINE(37)-N6)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47739:SF1">
        <property type="entry name" value="TRNA1(VAL) (ADENINE(37)-N6)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase small" evidence="2">
        <location>
            <begin position="107"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="391" mass="44540" checksum="FC464BAE0D8C820C" modified="2022-05-25" version="1">MMEDNKSNNSKSDNIENTVLPPSPTPEKKKRNKGIFYFKHFPVYQDLAGIGQKVGTDALLVAGYTSIAKHKQSNQLEENRNSPNNKLNNLVWNETIPYDYQFGKSNQLINNDLPNDNWPNYILDIGTGTNVLSLMMGQRFKDATINSIDIDEMAIKQASFNIDHLYLEEKVQVSKNIYLFHTPIQDFVPGTTKTVELKKNVITNDNKSTGSTPTDGFYDLIISAPPYFPSDPNTDPVVSSMLTTRRIARHTHTLSMQDLVLAVKRLLRPNTGLFTTIVSVPDPANELEQMAQKHGLVCVEQVDVTDSPGTKLIRKMYTFKLAINTDDSNNNNNNNNNNNSSTQIETIKQNFTIYETTIKESTQRHHRKHSDQYKWLLNDFCNHFHKELDNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWX4</accession>
    <name>A0A8J4PWX4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF866 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003346</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0587 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000103">
        <property type="protein sequence ID" value="KAF2075370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWX4"/>
    <dbReference type="OrthoDB" id="10248838at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008584">
        <property type="entry name" value="CXXC_Zn-binding_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12857">
        <property type="entry name" value="CXXC MOTIF CONTAINING ZINC BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12857:SF0">
        <property type="entry name" value="CXXC MOTIF CONTAINING ZINC BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05907">
        <property type="entry name" value="CXXC_Zn-b_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141678">
        <property type="entry name" value="MAL13P1.257-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="156" mass="17891" checksum="0999A2E798952A48" modified="2022-05-25" version="1">MVKQSVFLKAELEGVSNIYPEPHKIWFVKLKCSNCGETPDNFIGIDPENIETVGKANVNLLMSCKMCKRENSVLIDPSIKYADRTIQSEEEFEVVRFDCRGVEIEEFDPRDEWFVVSESGKVYDSIDLGSEWSEYEESTSSSLTIMEIQSEIKKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXW8</accession>
    <name>A0A8J4PXW8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">MACPF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_003803</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000124">
        <property type="protein sequence ID" value="KAF2074884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXW8"/>
    <dbReference type="OrthoDB" id="21110at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742">
        <property type="entry name" value="COMPLEMENT COMPONENT C6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742:SF8">
        <property type="entry name" value="FLOCCULATION PROTEIN FLO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00457">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0204">Cytolysis</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035147759" description="MACPF domain-containing protein" evidence="5">
        <location>
            <begin position="22"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="domain" description="PA14" evidence="7">
        <location>
            <begin position="308"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="MACPF" evidence="6">
        <location>
            <begin position="509"/>
            <end position="860"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2074884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1145" mass="127976" checksum="D90CDF0600B4E029" modified="2022-05-25" version="1">MRYSILYSISYLLLFVWVSYSANPTFYLSSSSQCVGECGSEKQPFANFQDAITTLSTFKSGDLPTPILMVDRGDYYGDKNNEIIIDFDLEIRGLYEQDRTVIDCQSSTFAFKATGSTNKLTIKGIMIQNCIAPKGGAIYTDNRYTVLENVIFYSNNAMEGSSIYSQAEYLTIDSCMFVANQGLSSIVEIDKSTTNIMNSRFVNNSDGIMDLVCGDAADVYLEQGSVFKSTCNTDCRVYSIEQELCGATPSASDLEASLCNYDKKCDSLIENHINCAFDCVDAKIQCNNNGFCDAGETFKTCPSDCNSMLRPGWKLETYNYDITKPKHAYTNYTGVSSIEYLRAPSLLDFMGKTYPPLSAKLSSKISITETKDYYFQLRVSNLAAIVFLDGRVLFDSFFPQDNTGSMLIERKLLLSSERAHFIEVYFTVNNDFQRDLELMWKFDLNEQYTLIPSAFITLTETLTCGDGVCNEEPTTCIIDCHDQIEKDCSPTSPPPPLQEYYYPIQDTVGTLLNTQYLSTLPGLKYMSQGINLETGKSLPSAIFAYTYCDDYFSLAHFHYRDTVYTIPKGLHAQIAPRCTMDSTTSVFGTSDSKATNDAEEWGLDVNAAGGGSAPFVFSAKVSSAFKMSESTKKASDTQAKTNNILFNTKVACEVYKVNVDEPYRWSPRFIQDIGSAYEEQEGDTDQQIRKKTVANMVRVIEKYGAAYYKSATFGGQIDQSTIVNSEFASDLTSEELGKSVESTVAASLSVKVYPVKVNAKYSYSSTSKKENSKMASFEKNSKRTTATVLGGAPGSYGEEDANAFETWASTVDQLPYPISGKMAFLSDILPRSWYFKGSLSVRDAWLDAEELYYKGRVGIADLIYVTKEDIDALEPDQTFYLLEGKDCFIDFTSQMMIDISIANETGPESYQLVKYLSNGAWTLLRIKHNDDQGVAHIKFYNSNGAVYPCGNTMEATLTNLVTSRSYHLMKNTQIGGYSIASLVENRILIRFQSYFLTKDCYGCGVQITVIGSNGRASTIVDNRSLMTDYQHLQTSMEFEINAKNYIGQVIGLELSIATVAYSGEPDQSYYFSFDFDNIKVFQSCPNDQHSKCVPTNILQNTPFDRIGMTKAYDTRTYSFYGSPFISVKFSDAIRKVYFPLDLIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PY98</accession>
    <name>A0A8J4PY98_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Methylenetetrahydrofolate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001423</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000033">
        <property type="protein sequence ID" value="KAF2077298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PY98"/>
    <dbReference type="OrthoDB" id="41403at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004487">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:001447">
        <property type="entry name" value="Methylenetetrahydrofolate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF3">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE DEHYDROGENASE [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic" evidence="2">
        <location>
            <begin position="21"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding" evidence="3">
        <location>
            <begin position="155"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="332" mass="37647" checksum="F0603D66FF5FFA0F" modified="2022-05-25" version="1">MHSMITNEYKEIVENKKGKVDVTPIANFFRNEIKEQITKIGGKPRMVAFLTTDDQGALDYSKWTKIACNKDGIEFILKRIERVDLEDAIIEANQDDDVHGIMVYYPVFGGMLDSYLQDVISPEKDVEGLSTTNRFNLYHNIRYLDKEMTKKCVIPCTPLAIVKIIEQLGIYRENVELGQALVGKEITIINRSEIVGRPLAAMLANDGAIVYSIDINGIIMFEAGKRYGTIKMVETKITKEEAMLKSQVLILGVPSPNYKVNSDLIKEGTVVINFAGCLNVDEDIDSKTILVPTIGKVTIAMLERNLMRLYNNQRLKKTLPPQDQLNQYQYYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYA8</accession>
    <name>A0A8J4PYA8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carbohydrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_007044</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000355">
        <property type="protein sequence ID" value="KAF2071636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYA8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019028">
        <property type="entry name" value="CBM_49"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052879">
        <property type="entry name" value="Dd_Spore_Germination_Stalk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33239">
        <property type="entry name" value="CELLULOSE-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09478">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01063">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035304783" description="Carbohydrate binding domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate binding" evidence="3">
        <location>
            <begin position="197"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="178"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2071636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="291" mass="31976" checksum="82B798C20D8986C3" modified="2022-05-25" version="1">MFAKLALFICLLCCVVGLQGCDVFASLDPLFVEAGQYKCLTVSGVSVDSSTFEQKAYKGFIHFSRYPESMLRHTSPNLINIVSSNSVKCQNGLFQPFLNSNTKTNEFTIFKSNSTLRLDGVDSVLSCINPTTFLASYGSNTLLFNIKVNPIEVGCPVIQFGDQCTNDTTPTSSPTIAPVTTSPATTETPTQSSNNNLTIEIKQTYSWEQSGKTYYNYDVLVGNPNTFNVADFVIQPSSNFSPENIWNCKKNDDGTLSIAPGQSIGSNQKFTFGFTIKYTDFSFSVLSYTKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ36</accession>
    <name>A0A8J4PZ36_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_001866</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000047">
        <property type="protein sequence ID" value="KAF2076838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ36"/>
    <dbReference type="OrthoDB" id="26095at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053331">
        <property type="entry name" value="EGF-like_comC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24032:SF24">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24032">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035240821" description="EGF-like domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="957"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="3">
        <location>
            <begin position="636"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="4">
        <location>
            <begin position="732"/>
            <end position="938"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2076838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1013" mass="113110" checksum="3A1DF09180CE4E4B" modified="2022-05-25" version="1">MIKYISIIILLCCIFYINKVEGVNDSLPSNQRNNLATLISIYQLESVYSNSLNYCTNTTFIKCNTALTTVSEIKLKGNGATILSQNIFSPFNNLVTLQLSDTLLSDEFYNELPISINKLSIYNSNITTLPTSDSIRTITMVNVPIEDSLDAGQLHNIDSISITYTDYSLIRGVNLTLNNPNRPERYTFPRYFDIPINSFYNTSASNINSSTFFITIGTKWESQSLANLTYLNETMGSIKFSNFNQDLKPDIDLDKLSNITANQFSFFGVNTFISNELRNSFQFPKEPCQALEFKQITGVVDSNGVFDIRLNPNLQYLTILNSSLKYVPNLDHSYKVLDFSYNELSGPLPTSQNIIRSLKILLKANQLSGEVPQEYCFLYSDLSYNKLIGALPDCFVCALKSKMIKRYLFGNNFSNYQDTSTSFPLCSGITFTNHSKNELNAPIIINGTNLGWNYDDVIGSNYPFTILSKPELGFTVSIPNLQIISNTISEGQNSILNASKYFLELNFTIPNIQKSIQLTEPDLVIESIFAVRPYVNLGYTFIILVNGISTKQGQTVAKVGNLDCIITEISSNFIACIVYQQQMAEGDYLVTVSSNGKQDSIKYTFIRSPPFISAFHPPSKKGGEAIFWGDFGNDPTQTLYLLVGDENCTNIHLFNQSTLSCQLKPGDGLKQVIVNLNGTSWISSKTYFNYLEDEKTCTNCDHGQCNYGQCFCYEGYGGANCSNIIDAGVVERNENTVTISKDDYKFGFTIANITETDYNGVIQRSVPLTGWKITNDLGYKWTYATKFEKAIISYTIEMVNETKSVNFAGVEMQLTPGTVKLTANISNWQYLGSLNTIQMQVQSKVYSQENLSVPDKCGKPMSSLITADTDLSTTSLNYISIGIGNKVFYGKFIDRVLSDGKPTFARVTMNEKTNSSITVSINLPYCKECIIDPDFSVLVSVNQVDECGNELRASKKWVIPIAVVFSIVGAAIITALVIVYLRKKVYLSFSLKKGIVIVKKRKSTSKSEGSRMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZF7</accession>
    <name>A0A8J4PZF7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Acyltransferase 3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001650</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000041">
        <property type="protein sequence ID" value="KAF2077018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZF7"/>
    <dbReference type="OrthoDB" id="23568at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002656">
        <property type="entry name" value="Acyl_transf_3_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01757">
        <property type="entry name" value="Acyl_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="209"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Acyltransferase 3" evidence="3">
        <location>
            <begin position="73"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="420" mass="48559" checksum="F75146DBC80DF03C" modified="2022-05-25" version="1">MDPLNMSSASIENEQLLRDSIDTESNTAEEHELIEVESDKPSSSSSTQPIQVADTPTTHYKPLVSPQVTSYSKAVAICLVMYGHILVYRWWGLPVWLAPLFRVSTLSVGVFAFLSGYGLTKSIQKSGTDGFFSKRFTTIYIPFFVTTVCFTCLFRGSYQSLDNFTILLKYLSLYLDLASTIDGTMWFITFICSWYIIFYFVFRFIKNDHLRVIIITLIAIFAYYRFSQNTKYWRLWLVYSNQLFNIPLGVFFATYENNKYIQKFIKEKKINIPIMLVQTALIVILYWKVLYIGIQDYGTPHSNRFNTAQTVSNMLFGTWIPFLFTLYFNRSNVILDFLGGLSYEAYLLEGILIEMKYSKTRVEDGVLYFLITFACAYALKRVNQAAIQFTISTVSTISQKIAGILQRQSYHPLRFGTNRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZN3</accession>
    <name>A0A8J4PZN3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_002752</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000079">
        <property type="protein sequence ID" value="KAF2075949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZN3"/>
    <dbReference type="OrthoDB" id="10264753at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01927">
        <property type="entry name" value="cyclophilin_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000003">
        <property type="entry name" value="Peptidylprolyl isomerase domain and WD repeat-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF4">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE DOMAIN AND WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0697">Rotamase</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="110"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="9">
        <location>
            <begin position="485"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="24"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2075949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="645" mass="73468" checksum="4CF2351B93E8A4C7" modified="2022-05-25" version="1">MSSNDKKRSNQDGDESTDTTSQGHQDDSKRLKTTKSADHEKKKKNKELPFEQLYLDSLPNSFMYEKSLMHRDICTFVAVSKTEFIVTADILGYVKFWKKLPIGIEFVKTFRSHNGPITCISISFDGLWLCTVGFDKNVRIYDVCNFDLINSFKIPYLPLTCQWIYGPNSSKTLLAISSRESSDINIYDSTSESNSEKSLHCLKVHRKPVHLIKFNPTYHTIISADIGGGLEYWDHEEPFNEPNHLEFTMKLDTDLYDFMKNKTVPTSLEVSPNGKFLSSMGRDKKLRVFNYLNGKLHKMYDESYAMLTQIQKSEDPNYWIDSGDFGKRMAVEKDIDGMFDNFLENITTKQAPPPTNILFDESNNFLIYPTLIGIKIVNMITNKVSRVIGKIENSTRFLGLALYQGRNEGDVSLGTKKRDSEPDPTIFALAFKKQRFYLFSKREPEDTDNPELGRDIFNEKVLKEESLFQQSAAAARQIPRNAVIHTTLGDIHIMLYPDEAPKTVENFTTHAKNSYYDGVIFHRVIKGFMIQTGDPLGDGTGGTSIWDKDFEDEFNRNLRHDRPFTVSMANAGPNTNGSQFFITTVPVTRLDNKHTVFGRVYKGTDVVSAIEKAKTNSTDKPLDDISIVSITVTQDVPSEYSSRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZQ0</accession>
    <name>A0A8J4PZQ0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002248</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000060">
        <property type="protein sequence ID" value="KAF2076445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZQ0"/>
    <dbReference type="OrthoDB" id="73491at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="91" mass="10068" checksum="3B5C5C2C6CB3508F" modified="2022-05-25" version="1">MADKGSTPGIKGRDEKQVNDNVLMSPQPPRSRRVMGPSLTDTNDTEIMSPQPPPSRRVMGPSLNDTSDIMSPQPPPNESNGTFFNRSCRWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZU6</accession>
    <name>A0A8J4PZU6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_001628</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPL family. K-HECT subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000040">
        <property type="protein sequence ID" value="KAF2077061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="423283at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045322">
        <property type="entry name" value="HECTD1/TRIP12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057948">
        <property type="entry name" value="TPR_TRIP12_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HECTD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25579">
        <property type="entry name" value="TPR_TRIP12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="8">
        <location>
            <begin position="1435"/>
            <end position="1820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="735"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="796"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1046"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1112"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="11"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="141"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="172"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="194"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="739"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1046"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1074"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1112"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1123"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="1787"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2077061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1820" mass="200527" checksum="8FAA3164D61D2A6D" modified="2022-05-25" version="1">MDKSSRKRKTTSSSSSGGNLESTASLTTSSPSPSTKPTSTTTTTTTTTTSSPKTSNIRTRSQAAAELAAVTHPAPTTSTTTTATNKPKQPVKKQKTSTTTSTPINKGKKSKSTPTTPTTTTPTLASSTTPSTPITRRRANTATSTPTTTTTTTTTSSKRSSSKMSNKQSSSEDINSGSEQQTTDKSTPLSPPATTTTSTSNNNNTNSSGEEDEGHGFDNRALQGLMRNLSGSLLDELTPFRNGQNPAAGLINTLIEGRNRSKQLLVGLKSDDEGKQMESLAELCNLLSIATEETIAGFPYDSFAPALINLLNMDYNPDMMILAGRALSNMIEALPSSVSSVINHGAVPMLCSKLLSIEYIDLAEQSLQTLEKISIEQPTAVLRAGGLMAVLSYLDFFSTGVQRMAVSTAANICRQIPQDCFNLVKEAIPILTNLLQYSDQKVVELSCLCFSRLVDSFYDSPTNLEFITSFGLLPHLVRILSGMYSNTTTLTPTTYSQIIRVMANICHGCPNLTLILLQEGITLIIQTIMLPAIDSSSNTNAINRSNQQFYEILSLINELLPPLPTDFSNLLNTTSSASIRGSLSRKKKDSTESLVSPPEDPRVGIYREHPELLVTVGSNLFVILVEMFTSTVNPSVRYKCLGSIWKILYFSTSDMIKDLLKEFGFSSFLASLLGSRDMAIVAAALKIAYLMMEKLPDIFDRYFKREGVVYEIDRLSKLEPLPIDTGVIVIDESNEADKTTTPPATTPISIPTTPTRTIPPPLASSPFGNNAFFVGSPAIPNTPSASSSFMAAANTSSINNTPSNNNNTTTTTTTTTTTTANPQTPEEYKTFVCKEARKFKEKFFPKLYSNNNMLASAVDDNNCFSTKELSILIDLGKKLNSSENEQGIKVLQEIKDMLTNTEGISAFEFLHSDLIGSILSYLTRGNIKLHAECFSKVFLTPYQKFPSTSGMTPLKSSSPNPLVLSLLIEKLHDALNKVERFNICISEVSGTSTGLKYLTQPFKLKLTKEGDDDNLKDYSSNVVLIEPLATITAIEEFLTSKAISTPTATTPLSTSTTTTAAAAAKTDEKKPSDIMDEDEDDEDYHDYEEDELLNGDQNETETDTVHDVKLNELSSSGQNFVPSNSPSSIRKSSNSSVNNTTTTTTSTSSPPATTTSTTTPAATPVATTPTSNKIGVFIDGQRLSNSMTIFQAVQKIAKASSALINQNSNTSNSENDSNNYIPTSRLWENTHVLKYRFLKQSEIDQPNAQSGSTNSNEPPIKPMGSSKLIKFLKDEHTFSLTSNDTTYEIIALLRCLYMFSSNPLENENRMKLPLLNGSEFISQKLTAKIMRQLQDPLALCGGAFPEWCKQLLNSCSFLFPFDCKRLFFYSTSFGIARALSTLQQRQDLIRQTNTSSSNERGDVRVGRIQRQKVRIYRQKILESAIKVMELYGKSKSMLEVEYFGEVGTGLGPTLEFFTLVSREIQRKDLKMWYSNIESNSSNSNENDSNNNNNSDNQYIYSSGGLFPQPMNPKSELPKKLLEHFKFLGLFIAKAFFDQRLVDIPLSRAFYKYLVNQELDFNDLRQIAPEYANSLNNLYGLVVKIKEIKERSDLNEQQKNLEISKLRIEGAKIEDLCLDFVLPGFAGWELLPNGENIPVTLDNLEEYLNLILKNFLYSGVVQQFDAFKDGFNQILPIASLEPFSLNEIESLLCGALTGSDNDWCIESLMDSTKADHGYTISSTSVQNLFTIMSEFKPEERKQFLLFISGSPHLPLQGFKGLYPRLTIVKKHHYPPLTPDDYLLSVMSCTNYIKLPDYSSKEVMKEKLLYAMKEGQSSFHLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0A6</accession>
    <name>A0A8J4Q0A6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Oxysterol binding family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000003">
        <property type="protein sequence ID" value="KAF2078558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0A6"/>
    <dbReference type="OrthoDB" id="14833at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF152">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="402" mass="45463" checksum="385F30C0348C0361" modified="2022-05-25" version="1">MEADPTLMSYADLHGEWENREISIFGVVKSFISQLSIGQELTKVSMPSIFLMPYSILELAASRYLKYIHLLISAQHEADPVRRMSIIIQYFFSCVRDGNFTKKPYNALLGEVHKCFVNYPSIDGNSTSQARFIGQQVTHHPPLTAFNITTTEGISLDCNVQFSAKFHMNSVSVVTAGAISIKIPVIDANGQTNIENYIIDKGLPDALVKNVIFGTRSIYWTDAVDIMCKDTNTSATLHFDKNEYVKGEIYSYNEDDVPQHQASLKGYISDVVNIDYLESKSSEASSATKSSSKKSKKKKHHHKHHTSGPTDTILIDTKTLNPNQTLYPRQTDTISSLVVWKDVTKNIIAGDMAASDVVKKEIEDEQRKRLQKTKEEEKRERTYFKFNDQDELWEFKGPNPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0R2</accession>
    <name>A0A8J4Q0R2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001048</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000023">
        <property type="protein sequence ID" value="KAF2077661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0R2"/>
    <dbReference type="OrthoDB" id="14809at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="38"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="92" mass="9868" checksum="72637BDA6B75E798" modified="2022-05-25" version="1">MADLGIKLRDAAKSGDAETVKKIVSECSADVINFKDRSGFTPLHMASMFDHKECCTILLEAGADNTIQSADGETAAEVGKVTLGNYINNFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q161</accession>
    <name>A0A8J4Q161_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">THH1/TOM1/TOM3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001850</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000047">
        <property type="protein sequence ID" value="KAF2076822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q161"/>
    <dbReference type="OrthoDB" id="20557at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31494:SF4">
        <property type="entry name" value="THH1_TOM1_TOM3 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31494">
        <property type="entry name" value="THH1_TOM1_TOM3 DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="228" mass="25856" checksum="F63F9424ED7F0DDE" modified="2022-05-25" version="1">MQLFYTFFLSEEIRLKNAKFISNSVYVFVSIYFCWQVIITSLCLTGDYKDAENVGFIVFFIFLFVVTGSNGILLLRAFKTQRKKSKVNFELMIKKTKILISGMVICVVITVVHDSVIMFDSPYRPYNFPLSVLLTGVADMLQLIVVMYVLADGKFHLYFTFKRVKTHGNIHGSSNDESSANAHKLSSVNINISTDHHQTNQPKDISIDIDSCTPQPHNDDTSSSKLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1L2</accession>
    <name>A0A8J4Q1L2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000060">
        <property type="protein sequence ID" value="KAF2076437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1L2"/>
    <dbReference type="OrthoDB" id="10289904at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36197">
        <property type="entry name" value="LRAT DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36197:SF2">
        <property type="entry name" value="LRAT DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="190" mass="21818" checksum="A2C9739DFF42D9BD" modified="2022-05-25" version="1">MNRLKLQEINKEKVYDFYLIVYANTSDQIIGSRIYQAGALENTLGSTNARHFSMLIDLTGETPEGEYPKGMEVHEVIWEPEVSQETASMTQVRCTKSEKAIKTIKIGNVAEKLSKICGGNSRTSNKLQCLGHYYCDLFERFIEQECKGDYNWSKLNNCHKFTKYCVQDLSLEWPESIGSPLDDYPFLLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1L6</accession>
    <name>A0A8J4Q1L6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial import inner membrane translocase subunit TIM17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_001679</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tim17/Tim22/Tim23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000041">
        <property type="protein sequence ID" value="KAF2077047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1L6"/>
    <dbReference type="OrthoDB" id="2261329at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10485:SF0">
        <property type="entry name" value="AT05822P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10485">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM-17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2077047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="168" mass="18207" checksum="150D428A4EF4117C" modified="2022-05-25" version="1">MELPCPEKIYSDVGAAFGIGLGLSTMVNIFFGLKRAPKAQMINYTFALIRKKAPTSGGNFAIWGAGFSGFDCSLSYIRKKDDIINPIASGAITGGLLACRRGFKATVSAAAFGGIFIGLIEAGSYYMRKRMQAQQMEMIEAQAAQAAQDQAMERAKEDAKKHNKQHQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1P2</accession>
    <name>A0A8J4Q1P2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_000415</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000007">
        <property type="protein sequence ID" value="KAF2078323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7743" evidence="3">
        <location>
            <begin position="387"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="2">
        <location>
            <begin position="755"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="1">
        <location>
            <begin position="1051"/>
            <end position="1254"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2078323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1301" mass="144992" checksum="15794E828A7DE6E5" modified="2022-05-25" version="1">MSWKIGEEPYADPVSRACTLVFMLTVEKDISASSIDGCSLSTTPSSSGGFCTIMSSEYNNTHFFAMITASVPALGSVYHQLDIQLSFGGLASESYTLGTTVGSLLTYFCEETPLSTLLFTKASDPKVLYSSNGVNNMQFTSYLDINTNVFKRPLVGLKPFTNNIQTEISPISLSKVFVKVTFSDSSSTPFTSQINIGLTLSNSISIPNPFFDLDYPSVTFQEFSPTQFEIPPFYGYHIKVLSDSVHNVIPPVNFLLIPLEGQDIHNMTYYFKNQQTSFQPKVLTNNAISTFWNVLPQSPPSSLYLDSLQQMELFTFKFNTPAKFSLSQSQNVGDTFYPSYPFGLVSYGTNTRNYDLSFAIRAEQKILDLFISAPGGNVFRFNANAPLDTVPPQFSSFNVLVLNHTHSVLRVGATDDMSGVNYIYIKTFLRDIYLNRNNLVSGSETDGIYEIIVPFKKSFLVNQAYILDITGNKREVTPLSLSLNYGLAYQPFGSALKLDSFSFNKLVVDVSNGPVDVIAYANITQTFAEREQQNSIVLNLFFNPLLDNPVVLGTYNDSLQLYQFPFTVQAKLIPGAVQYSVTLNQGYQFLSDYLFGIFDTVAQLMIKNTNDVDMMFPLVTKVQPSSFVPAPLLNTSGTPILLVTLSWDFEFYDPNGVKKVVIGISSERDVQGRNFTHDCQGKTLCQKTIDYQLDSNYCVKMNYYVQYVYTEDMLGNKGETVRYSNIGIHPFLKFDKSTIDFQEPTCPPAREENPPTIKDVQITGSSPTNSYDNVDVVFTIQDDDAGVSLAHTPVCYFHAYENKFIESVANVTKINSEKSVTYTCYFIISEFSPVFYLSIYGISDNLFNFIGFSTNNLAVSGFLYSYSLKTQRDYFIYIESTSSLEISTDELYLYGNFIFSSFPKVEIQTDIEKLTLTPNIATGRVLLLHNIDIKPSLQYNITVTNNGQKSNTVTVKGKLPPVTNTPIITPPVTISPTQTPTATCSSDCGSSLGYGACSRGTCVCNPPRSGLDCKSLITSPIITPNVDTPSVNLTLPSDGGQSESKSPTFTSFIAVVSIGEFNLNNELVSNHLFNYDKWQHIKEGSSKTDQFETIQYKYTINNELNTTITSTMYVFSQACNITFGNQELFMNPNTIKFTFNITQYPFALSTNTLQLVMTATLESSQSVSCSYKEFIDDSSNSQYLQIQIQDRSLFGRFIKFGMIDGRESTISNTQLDDNYGGSSLSKSKSDQSFIGLNIPYYTKYALLDPDFSVLIDSVSANDRENSICTFESKKKLTTAQIYVKVGPKIEKTRSEINAKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2E8</accession>
    <name>A0A8J4Q2E8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000025">
        <property type="protein sequence ID" value="KAF2077584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2E8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035182984" evidence="1">
        <location>
            <begin position="22"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="135" mass="15141" checksum="C9BB0D6C6128FA8F" modified="2022-05-25" version="1">MAKNSLIVLLLVLVVADFALSDRIYGTILLSRINNDTGMEKGLVLNLNTGTLYNQKQERYVVSKENLDKIILLLQGLNTETGMHVDLLNPNEPHTYTYLMVHSLSGRSITWQDGGEGNYGTLTIIEPLFERFTAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2G8</accession>
    <name>A0A8J4Q2G8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">YEATS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_001920</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000049">
        <property type="protein sequence ID" value="KAF2076794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1741717at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="YEATS_YEATS2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1970">
        <property type="entry name" value="YEATS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038704">
        <property type="entry name" value="YEAST_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005033">
        <property type="entry name" value="YEATS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055127">
        <property type="entry name" value="YEATS2_3HBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055129">
        <property type="entry name" value="YEATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058706">
        <property type="entry name" value="zf-C2H2_AHC1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23195">
        <property type="entry name" value="YEATS DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22951">
        <property type="entry name" value="3HBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03366">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25909">
        <property type="entry name" value="zf-C2H2_AHC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51037">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YEATS" evidence="4">
        <location>
            <begin position="414"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
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            <begin position="262"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
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    </feature>
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            <begin position="762"/>
            <end position="785"/>
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    </feature>
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            <begin position="823"/>
            <end position="851"/>
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    </feature>
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            <begin position="960"/>
            <end position="991"/>
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            <begin position="1003"/>
            <end position="1031"/>
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            <begin position="1128"/>
            <end position="1151"/>
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    </feature>
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            <begin position="1187"/>
            <end position="1345"/>
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    </feature>
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            <begin position="823"/>
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        </location>
    </feature>
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            <begin position="962"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
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        </location>
    </feature>
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            <begin position="1004"/>
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    </feature>
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            <begin position="1189"/>
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        </location>
    </feature>
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        <location>
            <begin position="1218"/>
            <end position="1234"/>
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    </feature>
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        <location>
            <begin position="1240"/>
            <end position="1257"/>
        </location>
    </feature>
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        <location>
            <begin position="1260"/>
            <end position="1293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1300"/>
            <end position="1323"/>
        </location>
    </feature>
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        <location>
            <begin position="1336"/>
            <end position="1345"/>
        </location>
    </feature>
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            <begin position="1388"/>
            <end position="1404"/>
        </location>
    </feature>
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        <location>
            <begin position="1417"/>
            <end position="1440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2076794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1654" mass="189366" checksum="0BB66B3F19B0CD3F" modified="2022-05-25" version="1">MDDDDTPMNDVDSSITNNSNINNSNNNSNNSLKYSNRRETTTTTTTTTAGAINRNNSNNSNTNVNHSNGLDIKSIQNEIQESVINTLKSELYQQFGLTGKGVIRNSSNNENDNNNNDEISNHLNLISEQILSLHQKQIELLTNRYSSMVETKFQELEDNLRNNIQVKFDTEMAFNNSSNSNSNVNSTPFSLMNASLSKLKRKLVINNEFDQETLLKRLKTLIEKEFPQEIKNKEEDLVEIDTRILKVKEMLHNMSRFRSSKTSTLYNSGSNNNNNNSSNSNSNSSSTTTSSGDKVPTYLYYRLTDGKFVQLICQTCKRDKYLSAIGFVNHCRIAHSIKYTSTDHAAEVCGIHVDESKIPLDDPCRSLPLQIYKSSSSSSTTPTSKPTTTTTTTTSKSLQLDIPLTDPTEPILNPSSRFYIKKRIIVGNTSTQISPERRGGDKSTHKWMVYVRGPSMESDISYFVKKVWIYLHESFAPNDIIEISNPPFHLTKRGWGEFPLRIKLFFHDTKNKPIDIIHNLKLVQIPIQYGVTKLGAETVVDIDLDRYFFDKRERMQKMLENNKDEKSNNDSNHEKSERSEKDEKSTDNNNNENDNNNNNNNFDDDYDDRCEKIDPDDFDQPIGSSNKNDDEEQEKKEKSMDKENRDKDSLLNSPIKKDLVKPKASSLSTTTTTLSSNTTTTTTTTGNNGLLGKSLTNIQKELRLKKEKEALIVQEMKKEKERERENIKKKKLEEQEREKEQERKEREKELERIEKEKEKIEREKEHIEKEREKLEKEREALERERNEKDRLEKLLKSNTVTTILPPILRAPLVGGALSSPPLISPLTPSKSTSTTTTTTTPTTGSASKKKKTLKSESIHNLLLRLASKYPIISSTHTIKTLSYRIAPTKAVWLKWTLGKQKSTEYRRAKVLKRILKKKSQKEFSIKSIMKWCRENHFTPLTPSQEERYSIKDTFIDPPKVVNTPSDSLATPPTANDSNTSLATIATTPTTTDQTMSIASIEGNQIQQEQQQQQQPAQQDVQELIQQEHQQIQQQQQELPVINHHHPIIPSPPMDVNMVLHEDAHSLFSSSGGHHDDLESQFILQHQFDHESHHNNHHHDHLHSLMPMDSLHVGPSPMEHPDSLLSTFPSAIDDLSLPPPKPKKPSKYVKKADREIEANANKPPLDIIPILPISEESINSATVVPHIEPNTHQQPESTTNVESSQPFPNVEQEQNNNMDIDKNTDFPFHQHDEGSHLGGEQLSSQSILNQPPTPQSILNQPPTPQPIINQPPTPQPIHNQPPTPQPIINQPPTPQSILNQPPTPQPIINQPPTPQPIHNQPPTPQNTTDTPIDHHPSSSSTTNMDDNQVYDFPLIETSLLTPLTPEPQHNLMSPHHPQDHNHHHHLHHQQQNIQHQQHPQQHYQNQQIQPYNQQHNNYQQQQYNSYQQQQQQQQPIDPNQPKTISVPHIQMFSGWDLSLFKPPIVNPTLRPNLPPGKKTLGPYPLFYCKFCGTGHQPDQFDQKQKHCSQSFVQSSYTSLAKSYPNIRITYPYNFDPESKSPNPNAKSSVHFLQNNVYHYDRYQIEWISYIMKLINIPRICDTPPDYEAEEVLYYSTFLFMTELIVDTLEVYQNEREKEPKEECIIKMIVPPHLHTAIKNNNLFNFLSNVAFYRDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2P9</accession>
    <name>A0A8J4Q2P9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rap-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_000490</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000009">
        <property type="protein sequence ID" value="KAF2078200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Ral GTPase-activating protein subunit alpha-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RALGAPB"/>
    </dbReference>
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        <property type="entry name" value="Rap/Ran-GAP_sf"/>
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    <dbReference type="InterPro" id="IPR000331">
        <property type="entry name" value="Rap/Ran_GAP_dom"/>
    </dbReference>
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        <property type="entry name" value="RGPA/RALGAPB_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21344">
        <property type="entry name" value="RAL GTPASE-ACTIVATING PROTEIN SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21344:SF1">
        <property type="entry name" value="RAL GTPASE-ACTIVATING PROTEIN SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20412">
        <property type="entry name" value="RALGAPB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rap_GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111347">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAPGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rap-GAP" evidence="3">
        <location>
            <begin position="1172"/>
            <end position="1388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="142"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="830"/>
            <end position="885"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1018"/>
            <end position="1049"/>
        </location>
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            <begin position="1451"/>
            <end position="1548"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="143"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="201"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="217"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="834"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1500"/>
            <end position="1515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2078200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1548" mass="172189" checksum="A4064B5FFBDC0FB8" modified="2022-05-25" version="1">MFIDWAASLNLIDPEKNTGILKDFPLGVKKILVQQIVTYLLDNPNGNSYILSTRDHVLWVMEIIEQGFLLPIEDHVIISQCIDLYKKWLFDAKHRPLPIHEDQDEFFLKKILQHMTLVFQTRSYPQNGVALSLIDSLSREVANSSSNSSSNSNSNNNSNNLSTPTKERDSISSSASGGSFNNSISSAQINSSGPVSNGKEQQQQQQSLPASQSISSGLNNPNITNLINSSGPVSNGSTGSGGGSGSSLNISAASSTPIITTTNTAVPSSQGSTNTIFNILYPPQVIPLNTLVEYHTILCHKVLQLYLDIGRAQSKNFSCETFELWITLMLATTDSILCGNTSTDELLAKNLCPLLLKCLFEIWLHSKTRNANLWNSLSKYANGWFHHMPMVNHWNILCMAFTTPLVSNIYTNDNQSKDTATVRLEDIIFEFEKDYLYYCWHRILNLISNPNTIKNPNIFAAAIGGVFQLVSIFLSVTADGNSILHIFGSWLFETVKSIKPGFDEGISLATEILSHIFLSCSSKINFLPIYLSCFYTSIAEALWCDGKILVSTVIHTQNIFSSELPGSRILIPSYLRALNKILTVAPAGAQSEYLRKSGIKILGSILSLPNRFEKVTFFNFFPGRPIDPYPMLPNDVITKGNELFVPPSEINTFDDVKHHIAHLILSALNTEVSPNNLQILIWYILFYQLEFEHQNVSNLPDGKNSTTAFIQASINILLKKSASFSNQWPHEVICCSFQLLADLTQFYQQIPNFSEIATLVVRKLCKFITFKCKDQNMSQETEDVICLAVSVISDWVIVSPWVFEGNYLSDTSTLYMVFNALSVALGAKNPNEETSSNTSMSSSQQSGGSSSGSGSTNNVAPSPAKKPQDAKAIPTPAPPAKFGQNLPPKVKEIAQCSLKTILNRICYFPNPHHACPTNTSSKLIEFDILKQIKENASKYLSIDNYPSEQGLRFYSIDDQTIISIIDQPFSNNFNSGAANDAKVDPNSNNFVTIIIRDMSGKSIVNTQLAYLPFKPKELADDSKSSSVPSAADEAADEEIADSNNNGNTRANNIISARKNTIVTNEIAPFVSSFNENTEEFEDLSMYINKHLDENFTKLVNNQMTIEQDKLKSLEFSLNVKITINPPWVKSSYTGECKLQQARILLSHLGFLNYENKNKLIPLENSTQFFQSLHMLDQVAERAQAKIPILYVKKGDVSEDDYFNNLTVNTCSDYQDFINMLGWFVPVASHTGFLGDIDKKTFTHGHYTPYFSTHNKEMVFYVTTLMPNQENSTAQEHKKRLVNKSNACVVWYEGKIETYEKTLLDSIPNNIQIVITPLNNLLFRVKTLRKVTSSKVRTGPICEEVIVSKHVLASVVKLTIVNSNQSGLLGIDARLQHQFLQRKKLISDISESFKNDMAVNKFFSYHFEPLEQGQLYEKVELPGTAENKTGFRFVKSSSVQHRNRPCSVQVGSAPILSHLPPPPASPSVTGNSGWGEGKKVEPSALRTSSSKLDSFLNLGSGKEKESNKEKEKEGSGKEYSASNTGTLPRPSFLSRRPAMTLSNEEDSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q382</accession>
    <name>A0A8J4Q382_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BTB/POZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_001483</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000035">
        <property type="protein sequence ID" value="KAF2077229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q382"/>
    <dbReference type="OrthoDB" id="14616at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00121">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF3">
        <property type="entry name" value="KELCH-LIKE PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="66"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="MATH" evidence="4">
        <location>
            <begin position="348"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2077229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="575" mass="66233" checksum="A00847CE6160D50B" modified="2022-05-25" version="1">MDVFSMGPIGLELKNISSASSEQFRQWSSSELVSSVQLIEQDLNQQYKFVDESDCLQPSFEKNITHDLSFLLPTTDQLQVSFCPSSEDEDSEFKNRIYAHKSKVSAKSDYFRLLLTNGMKESSQQEIVIPNVSVDIFFEILIFLYTSELSVNQYNLNQIFLVCDQFLLEDGKQVCRDYIKQIEPETLEMLLLEDDSLSTELTELYIEYAIDHSNYFLSLERVSKLSKDTLIKILNNSDITLKEIEIFKSVLEWIKANDQQESKSCIAELFHLIRFPLMDYQDMIDIVEPCPLVPVSLIIEAYRHLSKPKMLPLSEKDLENTRLNPRKKVSLAPGVDVFSLKCSEHKNCQSVSWPIHNFSAIQGQKHVSNTFEMCGLTWKMWAYPAGEAKHMDSFSVYLEAVRVKERESYDFLRNTTFFFGLVNQKNKLLCRQYPSSPNVLFNNEKSVWGNGLVELKYLYDKSLGYLDNDCITVQLHILEFNKQMSKHWMGNPRRQLNQKEEMSAKIFFDSTDAISLKRAPSIEHSQSNSLNSVLNLHGQKTPNPFSFSIDLLDIGFSSSSSSNNSEKSIELNKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3B1</accession>
    <name>A0A8J4Q3B1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000015">
        <property type="protein sequence ID" value="KAF2077950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3B1"/>
    <dbReference type="OrthoDB" id="8119704at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46118:SF5">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46118">
        <property type="entry name" value="PROTEIN ABHD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="59"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="388" mass="44548" checksum="E8204BC1838F7290" modified="2022-05-25" version="1">MNKAFGLPLHFNKSSLSIKYNPSTLFTSSLYNPLLSAGKQQQPVLNRDVVHNLNKTKYSLIFLHGLFGSSENWRLSNKLLLDKFNKHQQLDNSSIGNAYQHNDNNTLDIDLYSLDLRNHGGSPHSNEMTFDNINDDLSLFIHSNIYNQSNSSSDIDHKIVLVGHSMGGKSSMLYSLFNPTYVDGLVCVDVAPSNYVGIHNHDSKFLSMFEASKYFAQHDIDKKKIEDIMESFNLDKGERLYLLNNLVENNNNNSLDKYSWKLNVQTLYEYQNDMLSFPNDIVNRVEPFHKKVLFIGGGDSHYLLPKYRGNIQYLFPNNEIHFIAGTGHFAHAQKPKTFVEYLYRYLFDLITNNNNHIGIQNSNNNHINLTNIDLSLMNNNNNNNNKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3J8</accession>
    <name>A0A8J4Q3J8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_001216</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000027">
        <property type="protein sequence ID" value="KAF2077517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3J8"/>
    <dbReference type="OrthoDB" id="1924287at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000004">
        <property type="entry name" value="pre-mRNA-splicing factor CWC22 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050781">
        <property type="entry name" value="CWC22_splicing_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003891">
        <property type="entry name" value="Initiation_fac_eIF4g_MI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034:SF3">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWC22 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02847">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00544">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51366">
        <property type="entry name" value="MI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MI" evidence="7">
        <location>
            <begin position="539"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="481"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="734"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="57"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="126"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="488"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="741"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="755"/>
            <end position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2077517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="781" mass="90523" checksum="DE7F063F00AC42B4" modified="2022-05-25" version="1">MKGEEGEIDSPKDIDHNSASSEKKRNRDDYDDDRKRTSRSISRSRSRSRSRSRSRSKSNDRYKRRDGSKERYDSRERETYKNDRKRKDSRERDTSLDDNKKKYSDTSRSSRRSRKHRSSSSSPSRSRSRSRSNDRYKRKSKRDHRSSRDSKKRSSKRSYDSSSGSDSEDERGKDKEKRQQSSRKDEQIKRDEKIQEKIQEATMKERSSGVYMPPFKLAMMKQQVEDKSSAEYQRMEWDALKKSINGLINKVSFSNVKNIAVELFGENIIRGKGLLCRSLMRAQQISQTFTNVYAALMAIINTKIPDNGELLLKRLIEQFKKSFRRNDKPVCIASCKFIAHLVNQQVCGILVPLEIITLLLDKPTDDSVDVAVDFFTECGQAIQDASPQGFNSIYSRFKAILHEGEIDKRTQYQIEELFKLIRKSFEGHASVIPELDIVEIEDQITHEHLSLEETYDVEDGLNFFKLDPDFVEHQEQYKEVKRSILGDDSSDEEGGDDDSSGSDDDSSDDDDDQQERSAHTTINTNTTVIEDQTDTNLITLKRTIYLAIMSSKDFEECAHKLMRLKIQGHELEVVNMLIQCCSQERTYLPFYGNLGQRFCMLNRIYKQHFDACFQEQYSTIHRLETNRFRNIAKMYAHMFYTDALPWSAFQYIHINETETTSAGRIFIKILFQEMAEFLGIQKLNQRLTDPDMQIFFTGIFPKDNPKNTRYAINFFTSISLGALTHELREFLKNAPKAPIKSDSSSSDSSDSSSDSSDDSSDDSSSDSSSSEDEKRKKRRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3Z8</accession>
    <name>A0A8J4Q3Z8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TRASH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_000306</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eL24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000005">
        <property type="protein sequence ID" value="KAF2078439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3Z8"/>
    <dbReference type="OrthoDB" id="10262490at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00472">
        <property type="entry name" value="Ribosomal_L24e_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.170.20:FF:000001">
        <property type="entry name" value="probable ribosome biogenesis protein RLP24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.20">
        <property type="entry name" value="Ribosomal protein L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00773">
        <property type="entry name" value="Ribosomal_eL24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038630">
        <property type="entry name" value="L24e/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056366">
        <property type="entry name" value="Ribosomal_eL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055345">
        <property type="entry name" value="Ribosomal_eL24-rel_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000988">
        <property type="entry name" value="Ribosomal_eL24-rel_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023442">
        <property type="entry name" value="Ribosomal_eL24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792:SF8">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN RLP24-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01246">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01073">
        <property type="entry name" value="RIBOSOMAL_L24E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="TRASH" evidence="5">
        <location>
            <begin position="6"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2078439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="163" mass="19322" checksum="77B1F07186C7E653" modified="2022-05-25" version="1">MRIEKCYFCGGSVYPGHGVMFVRNDCKIFRFCTSKCHKNFKLKRNPRKTRWTKAYRRVSGKEMTVDKSLDFEKKRNRPVKYDRDLVNLTLKAMKRIQQIKERRESAYHKNRMVGVKANENKMKRQEIVDRIDIIRAPSALAKMKERIEKTKSKLGQLNTAMSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4I0</accession>
    <name>A0A8J4Q4I0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004915</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000183">
        <property type="protein sequence ID" value="KAF2073781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4I0"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052201">
        <property type="entry name" value="LRR-containing_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24111:SF0">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24111">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="411"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="424"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="459" mass="51131" checksum="C7CB4AE167601F3B" modified="2022-05-25" version="1">MWAVGSQLDRISAQLKSNSITSLFLPSFISINSSNINQLLDALSANTSLEELYLVDHNINKDELKKVGEVIGNHKSLYKVSVGHSKIGECVEELSGLLEGVLKSKSIRVINFSKQSLSGDGLKTIKDVMMVNNGGELHEINLADNQLTKESIPHLIEIINANPQLHTLELGENSFNTVTDELVESLSKSNIKKLVLNNCPIGDHGAVAIAKLMSNNKSLQQILLNNCDIQELGSSHIGNSLVSWKGSVFSASYNSSMFHHLFNDWDQTKLSELHNQGVLSDIESISLKGCMIGDQGLIKIVKGIKHFPRLKVLDLANNKLTAKGIESLESILLGPDSTIKELNISFNHLGKEGQDIIHNWIKSGELVSLDSLYISSIDLEFLDLFEIAKSLGDCNNSIKFLEAMNTIKPKFDEEVEKEENEKEQNDEEDDEDEEETIEDQFFELENTLKEEKSIKIKWM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q9J7</accession>
    <name>A0A8J4Q9J7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Isocitrate dehydrogenase [NADP]</fullName>
            <ecNumber evidence="10">1.1.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_001324</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19629"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="10 13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="10 13">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.42" evidence="10"/>
    <dbReference type="EMBL" id="AJWJ01000030">
        <property type="protein sequence ID" value="KAF2077396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q9J7"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004450">
        <property type="term" value="F:isocitrate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006739">
        <property type="term" value="P:NADP+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000002">
        <property type="entry name" value="Isocitrate dehydrogenase [NADP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004790">
        <property type="entry name" value="Isocitrate_DH_NADP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00127">
        <property type="entry name" value="nadp_idh_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006156">
        <property type="entry name" value="PRK08299.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11822:SF21">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NADP], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11822">
        <property type="entry name" value="NADP-SPECIFIC ISOCITRATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000108">
        <property type="entry name" value="IDH_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="6 10 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 10 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10 14" id="KW-0521">NADP</keyword>
    <keyword evidence="8 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="15">
        <location>
            <begin position="34"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="100"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="119"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="332"/>
            <end position="337"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="11">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="11">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2077396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="431" mass="48597" checksum="5EE3DFF7006065A1" modified="2022-05-25" version="1">MFTTRSYKVSTQLFNHSSWILQTRGFKKIKVTGPVVEMDGDEMTRIIWASIKNKLIHPYLDLKTEYFDLGIEHRDKTNDQVTIDSAEATKKAKVAIKCATITPDEARVKEFNLKEMWKSPNGTIRNILGGTVFREPIVCKNVPLLVPGWKKPIVIGRHAHADQYKATDFVVDGPGTLEMVFTAKDGKTVKRTVFDYKGSGVAMGMYNTDKSITEFAKSCFDYALARGLPLYLSTKNTILKKYDGRFKDIFEEMYQTKYKKEFDEKKIWYEHRLIDDMVAFAIKSEGGYVWACKNYDGDVQSDIVAQGFGSLGLMTSVLMASDGVVEAEAAHGTVTRHYREHQKGKATSTNPIASIFAWSRGLAYRAKLDGNAELEKFCKDLEAACIETVESGFMTKDLAICIKGNNVSPKDYLVTEDYINKVAESLDKKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPS4</accession>
    <name>A0A8J4UPS4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_009048</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000625">
        <property type="protein sequence ID" value="KAF2069631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UPS4"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF298">
        <property type="entry name" value="MIXED LINEAGE KINASE DOMAIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="535"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="826"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="227"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="279"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="321"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="424"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="362"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="328"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="427"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="854"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2069631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1114" mass="126007" checksum="92E78AB6A5F7BBB9" modified="2022-05-25" version="1">MDSPNRQQKQQQQQQLHQYIFQDDDCNTINDGYTEGYSEDGYKMDQVTLLKTSNNSISNQMKSSLDLISTLMNQVLDNNSNNNNININNVCSKNNNNSNYEEEMNFISSSDDSEDYKEENLDELSIFNVDIESEREKKVNQKLSSPPPFDPQTILSLSQSMLYSSSDSNLSSSPTLFPLSPTSSLPSHPMSPNSVQKTINSTCTTSSNNCSLSNSLFNIPNLPLSLSSSSTNTTNTNISSSTASSNTITPILSSSTTSTMTSSISTCSNLSNLSNSNGSTYSISNNNQNNNNNNNNNNNNNTSISPRFNQLISALNLKSLKQSHGSEDSSNGSNSSGNSNHGQQQQQHGNRITLDEVTMDTLRNFRKELNKLLDKRELLKEKKTLSSEGYNDQEFSNEELDDQLERINIEIGQIEAQMYMILPTPHSPNNLSPRSPRSTSSLFSASSTTRSGSNSLRKFSQELKMELKPLDMRAALYVYSPRRQATAKGKEFWDTNIDALVEEELTRKKIDQYLFYKQFIASNNYSSVEVDPSDIEMVGQIGEGNTHVVSEIRVKGVRLALKSKKAHVDSSDLQMELKYFQTPNHPNLSLTIGHTFHNNNPSIIVEYIEGESLFDLLHKENVPIEMPLFIKIAKEIAQAIYMIHTKKMIHGNLTTKTIYIDRLKNVKVCGMKCIDIDDPAILPRYRAPEVTRSEEYGPKSDIYSFSLVLWEMLTNTIPFRKFNDVSVAAKVAYENLRPKIPTSCPLVIRKLINRCWDPNSSARPDFNEIIKLFNHLEGKLFFSSRGSLWTSDKDQEAEREKQRKEKYNEIVEFLKMKDHMIRFEEIAFSEKLGEGSFAKVWLASWNGFKLAVKKLKNENITIDEKFMREVSNLIKAHHPNVVLFMGASVDPPCIYTEYMSGGSLYDVLHVQQVKFDIHMIFKMIHDLALGMNHLHCLSPPMLHRDLTSKNILLDSFRNIKIADFGLATPLNDEMTLAGICNPRWRSPEITKGLNYTEKVDVYSFGLVCYEIYSGKVPFVGLDGSVAAAKAAYENIRPEIPDDCPSPLSDLINRCWSSDPNERPSFGDILQELESKKEYITRMDLFDDFMMNDERENNYCIDIDLNPSTNNSDGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQP9</accession>
    <name>A0A8J4UQP9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide-like helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_007697</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000425">
        <property type="protein sequence ID" value="KAF2070981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQP9"/>
    <dbReference type="OrthoDB" id="2942533at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF8">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13174">
        <property type="entry name" value="TPR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="99"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="133"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="201"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="258"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="495" mass="56588" checksum="3E32598E7351A5AD" modified="2022-05-25" version="1">MNILTRITRSLNKQTSSPSFLYSAQSKQPYRRQYLFRENNLTSIDKQCYRYFSNSNNRNSDTASLNQLIINSLNTDGEDESLKLLNNVIENSSGGNGSSLAYRARGNLYAKIGDIYNSMINYQKAVECDPKSTKALLATAKIFISINDWDSAKSVYQHILSIDPNYLAAYSWLGFYELERSNVEKAKELFNKVLEKQANDSEALMGLGTIAIHNENIDQSIDYFNRAVQDYGNDIKQEEDDIDTIIDKHGLPNNSPYQFCFSKLGSLYRYKGDLESSIRNYEKALEIEANLDELLIITDIYLEQNQPLQALKYIDQSIMMDPSNVKAIVNKAVALLALQRYQESLQEFMNFKSLVDQDDPTSLIKRLVIYPKMITCCILILNQTTPISFDTTDSTTTSTPLSAIINSPPKDIDQLLEKSVRLHDYLVEKRKNTQPPQPFIDSLIDLTVSLRDACQDTIKVSQSNVNSEFKTQFQDHTLFFNSYYLITNKMTKFDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4US25</accession>
    <name>A0A8J4US25_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_005606</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000228">
        <property type="protein sequence ID" value="KAF2073081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4US25"/>
    <dbReference type="OrthoDB" id="10030083at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03390">
        <property type="entry name" value="PAP2_containing_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF35">
        <property type="entry name" value="RE23632P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="210"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="7">
        <location>
            <begin position="97"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2073081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="270" mass="30674" checksum="0915131798082055" modified="2022-05-25" version="1">MIQKVGYYTKHKWFTKNHIYDWVSCLSIFVVEQILTNFVINPRIRYEPTNFTLVDYPLIPDIVPAWLLLIICILLPLAIFGGFYAYYRNSHDLHHAALGLVQTFTVTMLFTDFLKIIASRYRPDYAARVALEGVASRLARDGRLSFPSGHSSLSFSCMAFLSFYIAGKTKVFRKDGGSMWKVLLCLVPFMISSLIAVSRTVDYHHDFSDITAGAFIGLSCGVFIYFVNFNSLFSKECALPKSRVSPNYAKDHGLLYSEREYTSLAISSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UTW7</accession>
    <name>A0A8J4UTW7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Lipase maturation factor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_010365</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lipase maturation factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001065">
        <property type="protein sequence ID" value="KAF2068308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UTW7"/>
    <dbReference type="OrthoDB" id="434126at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009613">
        <property type="entry name" value="LMF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057433">
        <property type="entry name" value="LMF1/2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057434">
        <property type="entry name" value="LMF1/2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14463">
        <property type="entry name" value="LIPASE MATURATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14463:SF5">
        <property type="entry name" value="LIPASE MATURATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06762">
        <property type="entry name" value="LMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25179">
        <property type="entry name" value="LMF1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="142"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="230"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="325"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="385"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="Lipase maturation factor 1/2 N-terminal" evidence="11">
        <location>
            <begin position="185"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Lipase maturation factor 1/2 C-terminal" evidence="12">
        <location>
            <begin position="428"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="19"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF25179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2068308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="600" mass="69407" checksum="82CE1C9DCD9141E2" modified="2022-05-25" version="1">MICVDSNTNNHNEGGVDNSTSFNTTTTTTTTATTTGSSSPLGTSVESLTDYITKKNKKKSIKEHQRKKSYFLSTWLFLKILSTVTFIAFFSAVIQVKGLIGDDGILPVSSVLEAKVSSKEINSIWILFIHPTKGMGWSVNDVLHGLCCVGLIFSSLSLFMTTTATYIGIMWFCYYCIVDVGQVFYSYQWDQLLLETLFLAIFFSPFNHFKYDDKPSTPIRYLLKWLLFRLMFGSGLVKLTPIWAGLQAMIYHYETQCIPNVFSWYFHQLPPLYHQFETMMVLAIEILLPPLYFAPRLFRIFASLVTIVFQLGIALTGNYNFFNYLTMGLCVLLLDDAFLLGFFFPGKFKHKLLEIESKRMKSIQQDRQADQRQEFSLSNINSKNLLVPFVFILVGTSSLYNTTRYFQKQTTPLLITRIHSALSLYHLTGTYGLFSHVSVERFEIIIEGSYDGETWSEYEFYYKPGNLSRHPPFVFPGHKPRLDWQMWFAALSKDYRSQPWLIHFMARLLQGTPEVHSLIEYSPFPLDKPPLYIRAQKYKYYFTKWKGGNIDIGVDNDSQWWSRDLVGTFVNSFSLDSLFYKSFILQNNIIKEKEKNNMNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UUF9</accession>
    <name>A0A8J4UUF9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Gelsolin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003265</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000100">
        <property type="protein sequence ID" value="KAF2075436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6375767at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051014">
        <property type="term" value="P:actin filament severing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008154">
        <property type="term" value="P:actin polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007122">
        <property type="entry name" value="Villin/Gelsolin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977:SF112">
        <property type="entry name" value="GELSOLIN-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977">
        <property type="entry name" value="VILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00597">
        <property type="entry name" value="GELSOLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00262">
        <property type="entry name" value="GEL"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gelsolin-like" evidence="2">
        <location>
            <begin position="648"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1413"/>
            <end position="1480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="263"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="408"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="468"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="486"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1419"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1468"/>
            <end position="1480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1480" mass="170304" checksum="F72D42534B9DB816" modified="2022-05-25" version="1">MEPKRTISKSKEGGSKSSKPPQHPNSKQQHSNRKTNRTNKKKNNNSNNNNNNNNSKKKTFTIEENLLAGTEGKKEEEELSQNEKDLLNKNVLDIKKKKQETDHAHRKEKQKKQEKKKLITETLKKKDELAKLQRKEEEEKKKQIEKQRLDDEKEIAAKLSELNLTKTIGHIGNGSSTMKGTSNVNHSHSGGKFSTFKRNTSMTEENILNAFKKKGSMTPSDANSLRKVSSHNSLGIKSSSSLFRTISVPRLSKQQISVLDIFQQQQQQQQQNGSASSPTTPGSPSSPRSMSPLLGHFSNSSSSLGKSLAQSGAGSRGSINSDLSNLSESISRYDFLNRPASIHKKPAQESLQRQSLKQDDYYSTQNTSKRWDDYVKTLLPTDQEILKRRKEESEEDSESEEGSISLESQEESEEESEQEESEQESEPESEEVESVDQEDLESEQESVDDESEQESQDEESEDEPIKSPPTESSQQPTTTNHKVEQESSASSKSVIIQKDGSTTPVSPIQENKRLSMTKEKRLSLSGENKRLSINKEHKRLSITKDEKRLSGSGQLTPKKSKEEDEGTKIINALLNKMSTRTFNTNTGDSSYASATQKPPQMVNIFKYGILDIDFSDLFGEEEDLKGFIMWSVHSFGLEEREYDDYNILYNKDSYLVMNLSNDNKNDANRMYNIHIWHGKESPIDRKGTAVMMAIQLATHMNGKVNLYTEEQGKETDLFIKYFINEDMDGMRYKSHGSSDKDTDFNHFENNEVKTHLFRIDIPPLDVEECGTIAIRRMGLSKKLIKESSKEISWLLVNNKKIYIHLGPKASLENKILIRQLSKEYENYFHASIPISEIYEEKEFTKYVKEKKTKIDRDKDWNVDEDDDTIVNLYKTCIKPNGRLGLEQISEEYPLHYNQLTNTEVYILDCTTDIFVWAPKGISRKKVNAARECAKVFFQEYDRPQWAEIHYFIEGNELPLFKRQFKDWPTPCHLANVNYTRPPLPSNISTTKLIYDFEYINATIKDEVIYPLLDNHPTDVVDVYDITLPELKFYKLDQSEKCNFYQDDCYMVMITTKDRGEKNWYQDYECKTVIYWWEGVNADFKGYASFIHGLYPIIAQKFIDRGQPMPRVERVTQRKEPSHFLKAFDETIVIHKGSRFDDPDITNRIYQFINEGPITYIQELDQNSLVLNSYNIYLIKSVVEQCIYIWKGCNNVMPHDELESFAQRLDEEFKVMLPYAQGDEPAYFWEMLPGLKDNIVMDFDATNDRLFKFYLSSKSQLKILRIGRMVPTDLDTQECCMLDTPESIYLWVGSGCSDTLENICHIFVKDYCKYYSIPQESIKVIRQYEEPLDFKHHLRGWDRKDDYIDPLETRKNQVALSHTLAYRKQCQEEQQLLIEYIEYANSLDDEDDLEDFETWTLIKKGLIDQFVDSPVSPQLNGNNSNGNGNHSNNSNSPSVASNGASSSSGLPPLVGKKQMVAVNSPSSKTPKKKKLFGLFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVA0</accession>
    <name>A0A8J4UVA0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010228</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000982">
        <property type="protein sequence ID" value="KAF2068445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVA0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="69" mass="8016" checksum="CBC8A9DB30A758C8" modified="2022-05-25" version="1">MTLFDEWQTLAYPKIREGLKELVLPKSFNNDIQNNTLPATLESLSILNHHYNKQLNINNLPTTLTSLEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVF1</accession>
    <name>A0A8J4UVF1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phosphoethanolamine N-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.103</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_002194</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N,N-dimethylethanolamine phosphate + S-adenosyl-L-methionine = phosphocholine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25325"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58641"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
            <dbReference type="EC" id="2.1.1.103"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:25326"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N-methylethanolamine phosphate + S-adenosyl-L-methionine = N,N-dimethylethanolamine phosphate + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25321"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57781"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58641"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.1.1.103"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:25322"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Phospholipid metabolism; phosphatidylcholine biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.103" evidence="5"/>
    <dbReference type="EMBL" id="AJWJ01000058">
        <property type="protein sequence ID" value="KAF2076516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVF1"/>
    <dbReference type="OrthoDB" id="18809at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000234">
        <property type="term" value="F:phosphoethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44307">
        <property type="entry name" value="PHOSPHOETHANOLAMINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44307:SF2">
        <property type="entry name" value="PHOSPHOETHANOLAMINE METHYLTRANSFERASE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="8">
        <location>
            <begin position="50"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2076516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="280" mass="31688" checksum="A42EB2C3347CBDFC" modified="2022-05-25" version="1">MIASEQYSYKEQTANGWGDAIKSYTLFAQNFTSFYSTDAIDLTLGKDSRSSNIKVLDVACGTGQLTFKAYNKLAKGQNNYVLGTDFSNVMIDFMNHAIQENQLKNIDAKVMDGQNLKELVDNTFDYAYSIFGLTFFIDRSKGFKEMHRVLNTGGKICIGTWCHDAAYFKILKEVSAQFLAEPLPFEGSAFSLSDKDQLQSELEAAGFKDIEIHTTVHNLSLNNYEEVFDYFENNPIYLRVKESINEAQRTDFDNRIIQYIKTTYNSLHLPAGAFIAIGTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UX38</accession>
    <name>A0A8J4UX38_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BCAS3 WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008235</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000491">
        <property type="protein sequence ID" value="KAF2070440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UX38"/>
    <dbReference type="OrthoDB" id="25778at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045142">
        <property type="entry name" value="BCAS3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048382">
        <property type="entry name" value="BCAS3_WD40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13268:SF0">
        <property type="entry name" value="BCAS3 MICROTUBULE ASSOCIATED CELL MIGRATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13268">
        <property type="entry name" value="BREAST CARCINOMA AMPLIFIED SEQUENCE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21034">
        <property type="entry name" value="BCAS3_WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BCAS3 WD40" evidence="2">
        <location>
            <begin position="66"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="BCAS3 WD40" evidence="2">
        <location>
            <begin position="466"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="690"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="724"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="912"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="690"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="764"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="931"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1031"/>
            <end position="1046"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1064" mass="116779" checksum="81D99900E6FC7FA1" modified="2022-05-25" version="1">MYHNNNNNNQGSSYGGMKAEEKSSYLNQSVISGYIKGISSYIPSSIKSSIKKQILNSPVVEVTDKDTLVGTYFDECDIQGEKHKLLINCYTNGFQIWDLNHPEGIKEILSIRDGLIKFCKVLTNPLEPDEPSSPFYGKRPLLAVVSGEDNPKITRNMIRIFSLQTTELVNMYKFQSPIYNVLSNRDLILVILKERILGFNPLNMKKHISLHCYPSVNQFGAIALGSRWIAYTDNEANYHSSFNHHHHHHHQQQQQQQQRSNVPQNQTFGDTAVDVASDLAKEVATKLYYFSDYSRKKVNSYLYPESDSSNAMNNGQLTTISNNEPKQPEANTIIVVFDFIKGKTVSLIKPPHSHPISYLAFDPSGTLLFSNSTEGTKVNVYQIIPFTNSLNSLSSPTSYNINGNVSGISSGGSINNNNSNSSSGGSGGLILSNKDNGVVNSNSNSNSNANIGNNNMTNNNFENSYRQIYILKRGITNASIQGISVNETSKWVALTTSRGTTHIFAINSLGGDVNIHTHITKSPNSKKHFDYCSSVLNFTPSLLTISAIDRIKLGNNNDETISNTILAGNACFIDSNTANLEKLFVVSPTGQLILYELRPQPPPIHSDVEENTLCLSLTPVAEWDVCRKIKAPEFKSQYIPYYSSTSIDEYPKDSEARWLYNVELITHSQEIRAIWGVPQFTFRSASVVDSQSNQNNNNSNNNNSNSIESFFDQDYPVGEAIKFEKKKSSSPRSADFISSGPSQFSPNDGFESKSPSSYDTLPPNFINNNYNSNNNNNNINNNIINNNNNNNNNNNNNNINNIMDDDDDMEIIEKLNEAMKTPITKSAFPSTSTSPPTAIPMMGPGSGLPFGEDSPQFRSNNAFKPPNLYGQSPDGIYGGKPSFSFDNNASSNNIMNGFGDHNLIPNPKPISYAIPPTFDDEDDDLHLPSYPKFSPPSNSNIPSPLSNQFNDSPPIRSSGNINNNNTNNNNTTTTTTTTATTTTAASNNSNNISNSSSSSISTNSSQISPPTPTPPIPIAKKPAGKEKEIESSISSTSSTSSGASSSPKTILSSPPKPSANKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXB3</accession>
    <name>A0A8J4UXB3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PA14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_008429</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prespore-cell-inducing factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000518">
        <property type="protein sequence ID" value="KAF2070258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXB3"/>
    <dbReference type="OrthoDB" id="17079at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011874">
        <property type="entry name" value="Fibro_Slime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011658">
        <property type="entry name" value="PA14_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051154">
        <property type="entry name" value="Prespore-cell_inducing_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001673">
        <property type="entry name" value="S_mold_repeat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02148">
        <property type="entry name" value="Fibro_Slime"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31137">
        <property type="entry name" value="PROTEIN PSIB-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31137:SF5">
        <property type="entry name" value="PROTEIN PSIQ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00526">
        <property type="entry name" value="Dicty_CTDC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07691">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00758">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035144872" description="PA14 domain-containing protein" evidence="5">
        <location>
            <begin position="26"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="PA14" evidence="6">
        <location>
            <begin position="111"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="409"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="409"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="451"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2070258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="516" mass="55130" checksum="D39AB7F006380F35" modified="2022-05-25" version="1" fragment="single">MASKYKQALVLLLIVFFSNIQNASSQTTLDLSGVIRDTTPSRNPDFQYNVVTAQGIVLATLGADNKPTYCCGDNSYPPIMPVVHNQTTFFGFFNDDAGVNIAIPYTVTLTQDPMNPNLYRYTNNAFFPIDGQGFDNATNFPGETVYTDAAGNPHNFHFCFEVHTEFEYRGGEIFTFTGDDDVWVFINDQLVIDLGGIHRALTAAVNIDTLGLTIGQTYPFDFFYCERHTTESEIAITTTLPFVCPFYDACGVCQGDNSSCCLPSNCDDIPGLDVLLCITQTCTDNRTCTATPFNCNDDNICTTDACIPGAGCSFTAVVCNDNDPCTYDYCSTANGCVFVPIGGCVACENTTCQSEDLCIPLVCHPFNGSTCISNPINCDDGNPCTIDLCYLGVCNYTVVPDCPRITTTDTTTTTSTTSTSTTGTTAPATTGTTAPATTTTSSTTEGGGTGTVPTTTTTTTTTTTSSTTGPPAPPPCVCPPGYDCRLEYHPTAGWLILCTQRNTTTTTTSTSTTTRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXE1</accession>
    <name>A0A8J4UXE1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Calcium load-activated calcium channel</fullName>
            <shortName evidence="14">CLAC channel</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_008319</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Calcium-selective channel required to prevent calcium stores from overfilling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the TMCO1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000502">
        <property type="protein sequence ID" value="KAF2070363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXE1"/>
    <dbReference type="OrthoDB" id="342726at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032469">
        <property type="term" value="P:endoplasmic reticulum calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002809">
        <property type="entry name" value="EMC3/TMCO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008559">
        <property type="entry name" value="TMCO1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20917:SF0">
        <property type="entry name" value="CALCIUM LOAD-ACTIVATED CALCIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20917">
        <property type="entry name" value="PNAS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01956">
        <property type="entry name" value="EMC3_TMCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF023322">
        <property type="entry name" value="DUF841_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01415">
        <property type="entry name" value="DUF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 14" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="10 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="33"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR023322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2070363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="182" mass="20374" checksum="0AFE6AFCB174F520" modified="2022-05-25" version="1">MTSDILFIIFVSIASSLASEAFSWVLVYRTDSYKKSKQTIERLEAQIEKLKEAESSASILKKGKDKRLERAEEALKAANKDLSFSKMKSMFAVAVSMIALFSYLNKGFDGKIVAKLPFEPIGFIQGLSHRNIEGNDMTDCAMTFLYVLCSMGLRNNIQIILGTEPPKTKNANPFLESLEKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXT6</accession>
    <name>A0A8J4UXT6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Structural maintenance of chromosomes protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_007789</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMC family. SMC5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000435">
        <property type="protein sequence ID" value="KAF2070888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXT6"/>
    <dbReference type="OrthoDB" id="10254973at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45916">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45916:SF1">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad50/SbcC-type AAA" evidence="6">
        <location>
            <begin position="60"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="224"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="657"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="10"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2070888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1084" mass="125814" checksum="84B8C5C8F665E816" modified="2022-05-25" version="1">MSSRKRVVDDVDSGQEEEDQEEDKIQRKKRNIEIQKTQNISQPTPSPNKTDEYVNGSIYRIKLHNIVTYSDVEFFPGPRLNVIIGPNGSGKSSIVCAIALGLGGSPNLLGRAKQLSDFIKQGTERGFIEIELFNDNGENFVIRRDLKKDNSSDFRFNGKQMSKNDVLKKIKELNVQIDNLCQFLPQDKVVCFASMSPKELLIETEKALAVDGMYENHMKLIELKKSQNEETNSLEEQKRSLDDLMRKNQALEEEVGKFHERQRYLDTVELLKKKKLWVDFEECKDAAKRHKEKKAELEADLKKKEDDRAPTIQTIAKLKEGLATFQQKNKEISTQILQLENHIQKNNMQKEDITLKIDGANQQLDLLSQRAEQKKAEVDKLKREIHENNQKITQLMDENTVKAQLEEKNQELRVLNSKLNEAQIQQRSASDNLTQLDREIHSNVTRLGQINNKREAKLNALRQNNPEVFKAYNIINQNADKFEQRVFGPVSLEINVENDDHAKYLEFIVPYRVFMSFIVLTERDKETFHQLMDKFKFRANVFYNRNPGEQNIHREIPLDRVKQYGITHYLDQTFTCEAPVRDMILDTSHIYYILAGSKSTVGKEKEILKANPTLRNFFTSEEYWSRTKSKYGNQSELVRHSSLKPSDLLSGINLTEKVELETRIAELKSIASQKKETLSAMVAEQRTISGKMKSIQEERSRISQINDERRKLYAKITHTNRKIQDLSHEEDEEKIRARLKKDIEENHKRRVGILTAITNHLIKQCTLYLERDHIVIKLSAFEGRVQFEQDKLDNTASAVDRLRGELNIVTREYTKALGEAKSKKALCDREAPFTQELFSKFQEFEVTTSEEIINEINAYETKAKFIISKNPRVLEDYENRKKEIESLTAKLEDREQYITNRKAELERLKTAWLGPVNRFIQDINSRFTKYFEAIKCAGEIHLGFNPLNPDDFAEYRIDIKVKFREEDKELKVLDAHVQSGGERSVSTMLFLISLQDLTVCPFRVVDEINQGMDGKNERMIFDQIVQSAARPGLPQYFLVTPKLLHNLNYSENTTVLCVFTGPWHMTQKQFDNNLKQSIANKVNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYL8</accession>
    <name>A0A8J4UYL8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000227">
        <property type="protein sequence ID" value="KAF2073095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYL8"/>
    <dbReference type="OrthoDB" id="10607739at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008843">
        <property type="entry name" value="Spheroidin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05541">
        <property type="entry name" value="Spheroidin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="497" mass="56470" checksum="02888DE42BA0C331" modified="2022-05-25" version="1">MESMKTSISAIGQSVKGAMTGEHKVVNTKLIRFSGYQVDINNKTYNTMGKVGGNDVTDWYLDLNKGMEVDPKTMEIKECRIESNNDADPNLPTCDVGTSRYRPIGLRVGTQENKFNTVFLGNLGYGHGNYTTDDGETKKADEKGIIVGGGSSGNDRFHIFMNPEKAPSKSSNVRSVKFFEDLKKWKCCVNEKGGLLVGVVGCQDRSDCDFSEQELTDAHSSIQKLNDFIKQHCSPSNKKYHDFKKLILTLHTTFNFVDWRMEGHLSEYPLLVATLGRAYHDLNREISEQGFKDRLWYTLSIPSTLGGFNETFVKRWRDLGMTDVLKNDNIQRPWMVGYLVSDINETEWDRNIKDIHEKYVMAPYSNTDTFDILKQMFLVPTADDVKKRLFNLTLINHEEPMSSSTETPNRRPINRDFVGEVSKVLISQGFAGLILLSDRIDCHFNSVKKCPPANIEYDETLSAFYKNQEDVKEILDLVGFKSQEITNIERIAHSMSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYS5</accession>
    <name>A0A8J4UYS5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Condensin complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_006728</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CND2 (condensin subunit 2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000323">
        <property type="protein sequence ID" value="KAF2071953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYS5"/>
    <dbReference type="OrthoDB" id="21155at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022816">
        <property type="entry name" value="Condensin_barren_su2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13108">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13108:SF9">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05786">
        <property type="entry name" value="Cnd2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="254"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="358"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="419"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="464"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="546"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="808"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="159"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="255"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="266"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="360"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="420"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="517"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="573"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="813"/>
            <end position="832"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2071953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="893" mass="101276" checksum="2430AFC14B66C578" modified="2022-05-25" version="1">MSSTTSDSTSLRKRRLSVVTNPNKKLKDDNNNANNTNNNNIVIEDNIELNNKIQDNINNNNGNSNNNNIVKRRKSVLPQQQQKVTRFAPDVLDKENKKEEQNNNLEDNEDYYTDLENLFNTTTTTQQQQQLAAVPKLKRKSVLPADITLPPSIPSIVTNSKKQQQQQPSRQPLNNSQISEVYKNIIKLSTENKITIKNTWELPLIDYIDNVIESNNFQEASCTLDASVQIYATRVDSIHTDTYRYLGGLNRVEDNDNDDNDDDDNNHDPNSDQVKRREKRKKRVDVNTLENNPENITLKSIDLAFTVDPLFKKTSAAFDEGGVKGLLLNHLSTYGNCRLIFDSNDFINEKVVDTSTADDIISSNPTSSNQQQESTTTTTTTTTIDISEWSSLFKYNSREKICPTLNGFNNWTVKSIVVDGDNDDNNDNNNDNGDDDEFQQSDDDIQQFYDSNGKINLENTTDEFENSGIHPIDFDDMPAIGGGYDDRDDFQPFPENADDEDDHYGGDGGVAYDDDYNNNNQQQPHQQQQQNIIIDNQDWEEFEFFGPGVTENWTGPEHWKFKSKKKQTTANTMDDDDEDNEENEKPNKRKKTSKVVYLDFDAPPPSESLFEPGKNTTLTNAALKKAAESSTILPPDIHYDIKMLSRLFNKPKCTIPPMSKRDTVFRRRTTISSTTLNNDNTLESNEDGFGNNMTLISNDHDLPFSQTNEDTHVYGGGYDDDYDEDPEERYGRGSAIYDPTEDIERNNSQLEFTNNNNNTTLPTSSNFGDMLVAEPRKVEKLEAKYVKVPKKFDVKSFKQNVWSILEKDKDQQSTTTTSATTPTSTIPSSTSILSTVNEKPEFANLLKELEEKQPDKKEELSVGLAFMCLLDLANEKNLVLAQTGIDNLFISTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYY0</accession>
    <name>A0A8J4UYY0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_006490</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000302">
        <property type="protein sequence ID" value="KAF2072193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYY0"/>
    <dbReference type="OrthoDB" id="10251809at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1170.10:FF:000002">
        <property type="entry name" value="Baculoviral IAP repeat containing 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056737">
        <property type="entry name" value="Beta-prop_ATRN-MKLN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF18">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24981">
        <property type="entry name" value="Beta-prop_ATRN-LZTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="656"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="360"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="426"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="547"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="375"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2072193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="701" mass="78419" checksum="50D70A1FDA33A18D" modified="2022-05-25" version="1">MSDNCYKNIGDEQQQQQQNSRTILKANRITFSSGAKLGGGFFSIEARWGHATISLGKRVYVFGGQGEALFSNTCVYDSTTSVWSEVNTVGKGPSARYGHSATLIEEGNPKVMIFGGRTNKKYLNDLYSLDLRTMSWSTFHFEKHTPDSRAGHSCTYVPSFQNSGVRMVLFGGNHSNKYLNSLYILEFPKSQADTIKWTKPSTKGTPPCNRGAHSAHYLKESHSVLIFGGFDGKRSMNDVHIFNVQDLSWSQIKTNGIAPSPRNGHTSVLVGNRYFVVHGGCNEVNFLNDVHILDTETFTWISQPVIAGLTLFPRFHHSSNLLDTGEMLVYGGCSSGILYSDMCQIDLQVFLPKLLPPPQQQDDLSAPLTPNNNRTTTTTTTTTTTTNSQQPNTIGIEISLCDPSKQQSKDGKSYLEVQLANALSLLKIEQHQKSELSNELSKLKSDRAEELINSLDDSSKFQKIENALKKEQTQKAKAQEAITKLTYSIQEKDSIIQSCQAIIYQVQQNLKDSNMPLDKKEGMGKLLSNISFDNTESIKTTKDNILNKSLELQVNDLTKELLATKKQLQEEKIKNLYWERVKLNLAERFKKDSIVVENLLGEQLGSLTLQDLNILEEIHHTGIKRVGYAKQDLLQQQLNRLQKEKDASSHSNGNTCVVCVDNSINVVLLPCKHSCICNVCSTKLKLCPLCRSEIKDTIEYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZ94</accession>
    <name>A0A8J4UZ94_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_004389</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000154">
        <property type="protein sequence ID" value="KAF2074295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZ94"/>
    <dbReference type="OrthoDB" id="20388at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05471">
        <property type="entry name" value="pepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034164">
        <property type="entry name" value="Pepsin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47965:SF12">
        <property type="entry name" value="ASPARTIC PROTEINASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47965">
        <property type="entry name" value="ASPARTYL PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase A1" evidence="7">
        <location>
            <begin position="85"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2074295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="426" mass="48619" checksum="B2484F77AE5DD03B" modified="2022-05-25" version="1">MNLLSKLFVLVVLVIYIFGNVSSFVLVNKRLEFDIQKVDKTKDYGEHLIPKLPLVPIQYRDQEDLSKSLQLDIPSDSIPFSGSEFGIVVTIKLNNVTQTLLFDTGSTLVIVPEKRCKSCTEFKTQFYEKGVDTETLKCYSETCNNTKTLPILCGGIDKDICTFQITYSDGTKYICEMMNDYISILENKIPFVFGSIIADIGKQLPNSYGILGVGRPCPTCPPTISDNISKYFNIPNILGVRINENYQGKVTIGDPFINGNHEKLSYTPMDSKRSYGYYVNPTKIEITKISNRSLILDSQELALHYFLVDTGSSLTYFSPEIYDRTVRFIRYNCFIDFKCHNIYENCFLISVDRFQLFPTIRITFKDNVTLEIPPEVYFTKIINGVHCIGIMRGATPNKSIIGLSFMRNNYIIFDKENQRIGFVEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZV5</accession>
    <name>A0A8J4UZV5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_010317</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001035">
        <property type="protein sequence ID" value="KAF2068357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZV5"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="22"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="119"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="212"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2068357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="303" mass="34257" checksum="07E9F34410448D20" modified="2022-05-25" version="1">MKANNNNNSNNSNYNNDIKSKLFESKYLLSGFMAGVISRTVTAPLERIKILNQIDSYLVNGSRYQNVWPALKTIVAEEGPRGLFRGNLVNVIKAGPQSAIRFYTYEGLKKMTQDETGHLSVANRMWAGASAGVVSIIMTHPLDIVKTRLSLGHPNQTRILETIKDIYKLEGLRGYTSGLSAAVLNIAPFSALNFTFYEAIKENTKKYIINDPPVYFSSLYGALSGSITMTILYPLDVVKRRLMLQRYYKDPNQHKNFIHCIYQIVKKEGIRALYKGLKPAYLKVIPTVSINFFCYELTLTLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V039</accession>
    <name>A0A8J4V039_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_003582</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000114">
        <property type="protein sequence ID" value="KAF2075105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V039"/>
    <dbReference type="OrthoDB" id="28764at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12155">
        <property type="entry name" value="PGDH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43333">
        <property type="entry name" value="2-HACID_DH_C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43333:SF1">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE NAD-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="4">
        <location>
            <begin position="39"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="5">
        <location>
            <begin position="111"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2075105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="322" mass="35819" checksum="9F56F63F56EF196B" modified="2022-05-25" version="1">MATSKPIICLFKAIHQFQFDRIKELAPQYELINCVTSVPEDLDTDAIEIIIGYNRDIVNRILVSEKSRLKWIQAVTAGIDTYDLETINKRGILFSNASGIHKESTAEHVLAILLSEYRGIREAVQDQLNSHWRKITTFGELSGKKILIVGTGQIGERLAELCKAFEMEVYGVNTSGRQISGFVQCVSQKNITSILGQMDIVVNILPLTPDTHHFYNAALFASMKDGVFFVNVGRGPSVDINSLIDAVKQGKIGFAGLDVFESEPLSSDSPLWSLSNVLVTPHNAGKVRNYKNKLFQIVEPNLISFLKNGTLVKNQIDFNKGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0D8</accession>
    <name>A0A8J4V0D8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Deoxynucleoside kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000751">
        <property type="protein sequence ID" value="KAF2069092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0D8"/>
    <dbReference type="OrthoDB" id="15417at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13238">
        <property type="entry name" value="AAA_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="25912" checksum="A205E39ED3FC91F0" modified="2022-05-25" version="1">MNSILNSTKSMITVLCLEGCHGSGKTELCKYFQKNGFKVLDEAFLDMPTFGIPNQSLTMETVWVSNWFQRLLKMEKAESNNPNRQPSIYIADRSPYSAILYSKNKGHYLDPIISEQIKELKKYTDIDIKTVYLRIDKDLLWKRIQNRLLKEPERVKYNEDNYQWMEETVNFYEGREWDFIVDNSLSQDKVLHQIIQPLNIDISIIKQQHQSDNTNIVSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0H1</accession>
    <name>A0A8J4V0H1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_003033</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methylthiotransferase family. MiaB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000090">
        <property type="protein sequence ID" value="KAF2075660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0H1"/>
    <dbReference type="OrthoDB" id="190098at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035597">
        <property type="term" value="F:tRNA-2-methylthio-N(6)-dimethylallyladenosine(37) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12160:FF:000003">
        <property type="entry name" value="CDK5 regulatory subunit-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.30.20:FF:000003">
        <property type="entry name" value="CDK5 regulatory subunit-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="entry name" value="Methylthiotransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01864">
        <property type="entry name" value="tRNA_metthiotr_MiaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006463">
        <property type="entry name" value="MiaB_methiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00089">
        <property type="entry name" value="MiaB/RimO family radical SAM methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43020">
        <property type="entry name" value="CDK5 REGULATORY SUBUNIT-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43020:SF2">
        <property type="entry name" value="MITOCHONDRIAL TRNA METHYLTHIOTRANSFERASE CDK5RAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00273">
        <property type="entry name" value="(dimethylallyl)adenosine_tRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00413">
        <property type="entry name" value="CDK5RAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01061">
        <property type="entry name" value="methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="MTTase N-terminal" evidence="9">
        <location>
            <begin position="109"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="10">
        <location>
            <begin position="254"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="8">
        <location>
            <begin position="515"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2075660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="616" mass="70411" checksum="682B23FA93B9D53E" modified="2022-05-25" version="1">MISRLGSTSRLQNIIKQSLIHRSTTCLYNRRYTINSNNSITETPTTDNKDIKEIVKEKAKQVPSLNEFLVRTAKEQEVIEKQKLESLGGQYEIPPYLQSTLSNDTGEGRSVFIESYGCQMNFADTEVINSIMRSAGYTLASSYKEADIIFLNTCAIRDNAESKIWLRLSELRMHKRKNRKYNKLIVGVLGCMAERLKQKLLESDMKVDIVVGPDAYRSLPSLLAKISDGESQTAINVMLSADETYADITPVRTSDNQVSAYVSIMRGCNNMCSYCIVPFTRGRERSRPLESILNEVRQLSQQGFKEITLLGQNVNSYNYIPEEEEESAQQEQERLKPREGFNTIYKSPKKGITFTQLIDKVAEIDPEMRVRFTSPHPKDFPDDLIDIIKKRPNICKQLHMPAQSGNSNVLKNMRRGYTRESYIELIDQVKKQIPDIAISSDFISGFCGETEEEHLDTISLLEYVGYENAFMFMYSLREKTHAHRTMKDDVSVPDKTRRLTAVVDTFYRVAKEKNQKEIGRYHLVLVDRKSRRSDKDFVGRTDTNKKVIIADTPISNPSTGEQDQPIAIGDYIIVQIMEGSEITLKGKPIMKSSIQHFTTFNINDHSHLNKHQSLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0V8</accession>
    <name>A0A8J4V0V8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Thioredoxin reductase</fullName>
            <ecNumber evidence="7">1.8.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_003756</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[thioredoxin]-dithiol + NADP(+) = [thioredoxin]-disulfide + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="8">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.9" evidence="7"/>
    <dbReference type="EMBL" id="AJWJ01000122">
        <property type="protein sequence ID" value="KAF2074923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0V8"/>
    <dbReference type="OrthoDB" id="371245at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050097">
        <property type="entry name" value="Ferredoxin-NADP_redctase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008255">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_2_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005982">
        <property type="entry name" value="Thioredox_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01292">
        <property type="entry name" value="TRX_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48105">
        <property type="entry name" value="THIOREDOXIN REDUCTASE 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00573">
        <property type="entry name" value="PYRIDINE_REDOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 7" id="KW-0274">FAD</keyword>
    <keyword evidence="2 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="9">
        <location>
            <begin position="57"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2074923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="367" mass="39643" checksum="C656503488428873" modified="2022-05-25" version="1">MLSSLKSTLSNRNSLVSILKTSISSSNSNRTLLFSNNSNSNNIYIKRHKMTDQKVEKVVIIGSGPAGHTAGIYAGRARLNPLMYEGFMAGGVAAGGQLTTTTEIENFPGFPGDISGTQLMDNMREQSIKCGTRIETQTIEKVDFSARPFKLYPEGETQPILTQSVIIATGATAKKMGVPGEEKFWSKGVSACAVCDGALPIYRNKHLIVVGGGDTAAEEATFLTHFASKVTMLVRRNVMRASKAMQDKVKNNAKITVLWNTELIEIKGDDRVSAVTIKHNNQGDKLEDIDAQGLFYAIGHTPNTAFLDGQLTVDETGYIITEPRTTQTSVVGVFACGDVQDKIYRQAITAAGSGCMAALEAERFLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1W0</accession>
    <name>A0A8J4V1W0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_002872</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000084">
        <property type="protein sequence ID" value="KAF2075838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
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    </dbReference>
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        <property type="entry name" value="NUCLEOLAR PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13102:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22493">
        <property type="entry name" value="PUF_NOP9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="6"/>
    </dbReference>
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        <property type="match status" value="2"/>
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    </dbReference>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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    <feature type="domain" description="PUM-HD" evidence="5">
        <location>
            <begin position="224"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="3">
        <location>
            <begin position="516"/>
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    </feature>
    <feature type="repeat" description="Pumilio" evidence="3">
        <location>
            <begin position="690"/>
            <end position="725"/>
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        <location>
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            <begin position="175"/>
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    </evidence>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2V6</accession>
    <name>A0A8J4V2V6_9MYCE</name>
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        <recommendedName>
            <fullName evidence="2">SCP domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Polysphondylium violaceum</name>
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            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000321">
        <property type="protein sequence ID" value="KAF2071972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="match status" value="1"/>
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            <end position="23"/>
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</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3B7</accession>
    <name>A0A8J4V3B7_9MYCE</name>
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        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
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        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
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                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
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        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="VPS50"/>
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        <property type="entry name" value="VPS54_N"/>
    </dbReference>
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        <property type="entry name" value="SYNDETIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SYNDETIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Syndetin_C"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Vps54_N"/>
        <property type="match status" value="1"/>
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    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
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    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 54 N-terminal" evidence="6">
        <location>
            <begin position="118"/>
            <end position="410"/>
        </location>
    </feature>
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        <location>
            <begin position="821"/>
            <end position="1054"/>
        </location>
    </feature>
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            <begin position="1"/>
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        </location>
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        </location>
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        <location>
            <begin position="767"/>
            <end position="794"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="17"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
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        </location>
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            <end position="753"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2069584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1067" mass="122068" checksum="9C4FC5878C2CD07E" modified="2022-05-25" version="1">MSNNNNSNDHYGQYDDNASTSGNSNNNYNNSNNNNNQYNTYSLNSSNSSINTTHSNTSNSNMKSNTSEVDLFALTSKLNTNSDVDKLMLNQKAHQEQKTMSYLNTKTEQEIEKENTIIKNLNPIYFEQSSDIVQHILDQIPGDCEIDHLDGLTVKISGYHEAVDSRFNDLIRKSYSGFVKGLSQVYEIEKDLQHSATMCKSGKEYLNGVMKNLTSFGFILLEKHRRRELTKFMLGETQSFKDISQIARQVDSLLNENQFTEALIFLNKYNQEISTYSAKYNCIKDLASRFQSSVDAIIEKIDSNFVKVCHGWSEKEFENIVDGYSRLHQVVRFREKIHQNFILNIDDLSKKILKTQLLANFERPDEVNFKTMKFTDMCELLKEDQYVPALLSLLNLLSDLMWSHYMMKNWILVKSQSKEGEEKNFYIDIFKLLYSNRKTMWNSIQSQIRHILNSFKPKFKIEEFLKVLDSINQFIEVGNEFSGEEANILKSIMKEKSLAYFQFFHKKRIDDLKTMLENEIWHKMPLPNNFTVLDIKEFGVVMKLNNNLNNSNNSNNSGGSNNKQQQQQQQVFSTIFKVTEQKQISLELKDHDIAFMNQFRENGNPFSSAESRRTLNEASGDQFDQQNESTNGTNQQTNSPLIASTTVNVLRFIGKYFQMMKVLQPLSYPIFTAIAQLLEYYVYTVFNFFGSSSSSMFDDSSNVNPMLKKTMQRLKAKFNSNAVPSPNNHGKMSSPYQLKNDNSKSPNLSNTKDSLLSVTTPSVLSLSTPNLTVGKDNRNGGAGNNSTTNGGGDNFDDGEISIQWVLPTLSELVDLTQSKNLYGLENKIIGLESMMFLVEAIMEAQPLVMSLVPNEKSSAINDFYVQTVSVVASLKNLCYKNITSQLLNLDNIQNLVTGIKWELKEAPTTESNNYISQIIIEFHRFTGYLDEISSRSGILTPKVKNLLWENTITFAMETLIESYSKIKKCNNYGRNLMIMDLKKLQAGLESLTTIRPIPHIQHVDQYITAYYLPESDLFSLARDSEFTCKQVISIVNVCGHLKKNEKQKLINDLEDLDKQRKLESKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V485</accession>
    <name>A0A8J4V485_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BED-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_005384</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000213">
        <property type="protein sequence ID" value="KAF2073307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V485"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003656">
        <property type="entry name" value="Znf_BED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02892">
        <property type="entry name" value="zf-BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BED-type" evidence="4">
        <location>
            <begin position="12"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2073307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="83" mass="9251" checksum="78F4A23FF9A9CFB0" modified="2022-05-25" version="1">MKARRTKKILNPVWQFFKRGDNKAVCNTCSYSYNISGANNSSGNAKKHLQRAKCSGGVQYCKEVLGITMPNNPSITISTKECY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4I5</accession>
    <name>A0A8J4V4I5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor eIF2B subunit gamma</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">eIF2B GDP-GTP exchange factor subunit gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_007917</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on the eukaryotic initiation factor 2 (eIF2) complex gamma subunit. Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-2B gamma/epsilon subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000448">
        <property type="protein sequence ID" value="KAF2070769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4I5"/>
    <dbReference type="OrthoDB" id="10250549at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002183">
        <property type="term" value="P:cytoplasmic translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051960">
        <property type="entry name" value="eIF2B_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056764">
        <property type="entry name" value="LbH_EIF2B3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45989">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45989:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25084">
        <property type="entry name" value="LbH_EIF2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="10">
        <location>
            <begin position="18"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="EIF2B subunit epsilon/gamma LbH" evidence="11">
        <location>
            <begin position="364"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF25084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2070769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="442" mass="49563" checksum="E9ECFB63DAB7F2EF" modified="2022-05-25" version="1">MTEFQVVIIATDKGIGNSKLAPIDDNLPHCLLPVANRPLISYQLELLEKAGFQTKNTPVLMLVSERSQGKIKQYVSEIYKGNIEVEFVVIKDDIGSCEMLYRIKDKIKSNYFIVMNGNLIVDDSFIRQMTDQHRANDSSFTVLLKPSTKPEVPTKTGGSSKQAAVVEAPSKQDNLFTDYIALDEKKEKIILMEPATEAEDNIVFSKTLLKNFPNLTIYNSLQDTQFYIFSRWVLEILVEDQKKHIPTFFDLKKQFIPYLISCQIPDNNKNLPASAINDTQELALTMSSTSSPFNAFSQLNIQKKKIVKCLAYLFTSGYCINVNTIKSYQMANREIAKGYTPYLPFEPRSEKNFFIDQTAEVTPTQVGPNCVIGTSTKLGAKCSVKFSIIGKHCKIGDGVRIENSIIMDHVSIEDKCVINGSIICNDAFVKSGSQTIGTYLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V593</accession>
    <name>A0A8J4V593_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000352">
        <property type="protein sequence ID" value="KAF2071669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="20982at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040031">
        <property type="entry name" value="Codanin-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28678">
        <property type="entry name" value="CODANIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28678:SF1">
        <property type="entry name" value="CODANIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="385"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="480"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="519"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="775"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="914"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1467"/>
            <end position="1488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="330"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="385"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="417"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="480"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="526"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="775"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="919"/>
            <end position="935"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1593" mass="181834" checksum="88ADF99A34AEA0EF" modified="2022-05-25" version="1">MSNNVKGQNKQQQQHQNQHQQQQHDVTTINPIEQLLINCIKGKRLPIAVVNVWLSSTKTNKYLSPNDYHSYKELTSTTPIAFSKSFLEYFKSQFGHMKSSIDQYINNTISINNSSNSDSSLFPKLNENIDVDHDDQDIHSSIDVCVEIYTSILFNRLYTTSLLTEIEYIIHMLTTLDIYFIKWRSTTATKATTSTNNNTFTDLSNNNLLFSSISWTVLFILSLLKQLQPLLFYFDPSFLFKLSENDMLKHYDPKWCLLIQETANHMIQENASIIKDRASNIQSPFRHHMEMDSQKKLRKTEDANIFSNQEKSLDSFYDLLRQFQLDSKSKSNSTSNNNNNQSGSGNSDLFIQKTKEFFNLLLKNNLWWFCDLFVDEYIKCMNDSNNSSNSNSSNSNPVITNNTSSGNTPTKPPPLQPNININSNNNINIINNTNNMTMKPNSNPPNNNNSSNTGATTAAPVNQWSQNKLLLLQKRLDNESPAQPIPQQQPVQQQPQQQPSSQNRKSFSYAQNILNTKNNQTSHHTQQQQSQQPQPQQQQQQQQQQQQQPSNIINNNNFNDPTRHLKYMISRFNDREKFFINIILNYDKQIFYHHLTASVIEKIIVTQSCQCSNLNHYHSDLHERNLCLKTLSKLLSFIYFLPTLSYNSLSASLSVPNFHDIAKRTHLMPPPIDLKSFLMNAIQQETLSIHVPLVIEFLRMRDYIGNENPYFMDVVNILKNIYNIPHLLSTPQQNNYGFTYLYITIELEKFFKDSMIDPFQYTHVSLPFKNNILMSPSSPHSSGTNTTPPTTPTKGSHSNTTPKKGSSSSSSLPTTPSSSNNANSGNSNSTNSNNNNSTNGVDNNQRSGYTVLWTFNNPITSKFVSLLYEPIIYQLKLQEQSKQSADEKGVNGSLSTLTTTVASTTLVPRKRITPMKMNNESENKPQESNDKIMERDKSKERQLEWWYFWNHPSIARISNLLVETLLPFFSELIFNQSIPIIIQHSNQYIENSSNETNNTSGNNNNSDPLSSYLLDSSRLERLIKTIQEEVKSKSLDKVSDYCRDNIKTLILLLTPTGIDETVLNVGTSIISRHLKTVAENVCLHQTLQKVKILCIEIITRKQPINATTTTKSVATLQQLLCLSLSDISRQNKQNFSLSSIKSNHVAKLMEGVSIRSLVESLERHKQETEIHIKEIFNSSLPNSSGNNNNNPTIVNYSENPIDFIKQAESLFIESTDLIKEITIKHSITILNLDKPGNLNSNSVKVVDMDHLQKALPSIFRSVLDMISNHLILNNFYQYVHLLSAMDNSEGNDKSKAIQQNDIIFNINSIAFSIDTLISNQPSLILPINQTITSTFTKSCQHIISQLVELNPEFNQLITNFLLSNTKIFSLINQTIQFLKNNDLNQNNINNNGGDNGDDKNDGSEKNNILLKINNCFGWLHCLDGILISLIQENTIQLVSLESSFVNLFDRCITFIYEFNHIGNSSSVNSSNNNSNNNNNNNNNSKNINNSEDNEKLLIGALVLDSFLKYLVEFISLYHYHFTSISINDPNNASKLVFNYDKNSFNLCLNSFIQRLIRFQKRDKHYYTAQFIDQIGKSIQFLTFKKNRLFPSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5K5</accession>
    <name>A0A8J4V5K5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000428</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000008">
        <property type="protein sequence ID" value="KAF2078238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5K5"/>
    <dbReference type="OrthoDB" id="127285at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008833">
        <property type="entry name" value="Surf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34348">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34348:SF1">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05477">
        <property type="entry name" value="SURF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="238"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="278"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="328" mass="38116" checksum="5522C698D404FE73" modified="2022-05-25" version="1">MSIDKQVESFLKEHKDFVLSEDGKRVKCTLTNHEMIVRLDVLNTHLQSKKYKLVAISNFDFKKYEENFITESKKNPKKLYCGLTLTELNRDPEEIEKHIAGRKYKLALEKKTVNKNRKKKAMSDSIDGEEDDEEEIEEEEEEEEEEDKEGEEGEDDDSFEFFDYDQPEGEEEEQDGEENGEEDDEEEEDDDEESEGDEDDEALYNAESDSDDQDIIGDMNYKGFDHEAALKDNIVSSKKPTTKAQPEKQQQQDKKRKNQPDQTPEKSSVQPNKKQKQEPQPQQKPQTNKTPQEKPQQQTKSKPQAKPQSKPQAKPQAKPQAKPQQKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6Y9</accession>
    <name>A0A8J4V6Y9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pesticidal crystal protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005292</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000207">
        <property type="protein sequence ID" value="KAF2073379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6Y9"/>
    <dbReference type="OrthoDB" id="23795at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.10">
        <property type="entry name" value="Pesticidal crystal protein, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036716">
        <property type="entry name" value="Pest_crys_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35598">
        <property type="entry name" value="ENDOTOXIN_N DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35598:SF2">
        <property type="entry name" value="PESTICIDAL CRYSTAL PROTEIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56849">
        <property type="entry name" value="delta-Endotoxin (insectocide), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="417" mass="47941" checksum="B035403F0B61DB78" modified="2022-05-25" version="1">MSEPNLTEIEKYNPAFAIGRAVLGEEYKLDNDDYLETIKGTIGCIPVVGALATSCFTIFWKKAFPVEDPYLRKEDFEKRLKRLISDMEKKMDKKIDEEVGKPISDSVKSYMAANHVSFRNLLMECLIFFGREDKIELLIKLYLQTVVMYIAFLRDTHFCGIEWGLPEEYVYGVDHIRSMEYKIHDTIRLTHEHFSKFFNKVNETIISDFDQELPEIKGDYRTLTSQLTYADVSRYKYNQIWLQRTNEEGEDGDGSYYLPISNRIYIADFSKMGFMLGIFENCEEGENSVNSVTGTIGLLSGEARGLYYDTFYFMEPKNLTFRILGRTDSNAGLEIVIREIPEGQVGHGPDSSRAVATKTHIEKIPLKKLFESSSGPMSEFVSKPYKFEQTGSVYFEAKVIGGDTRDLYTLEIIDTQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7L4</accession>
    <name>A0A8J4V7L4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004638</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000167">
        <property type="protein sequence ID" value="KAF2074069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7L4"/>
    <dbReference type="OrthoDB" id="16851at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="336"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="345"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="370" mass="42038" checksum="968E36D20FA00502" modified="2022-05-25" version="1">MSKRKAQDDDNLSGSETEDESTTTTMKKNKPLDQDEYDRQNQSIIDLLSHTNITELKEEDYQDWFNNIAYQLQNNVILYINEIPHLMIELEFYFKGYKHNDLFAHCDVMQLEKASWYFHRTNGTFRGGSFKGLDITFGCGDDVYGGILIRSLKRVDSQSTIVDGPSLCVDHILKLTKQADIKTFVATAGRSVFESKNPLLYLKMLSDVDSSAQSSLPKPGRIEDMFGSGRVGLTFKRYKPTMEYFIAQNYRFLRYPDKVKKGKHYMVLSDGLQGKGPVEIKNRVGSTVASIEKILKQITTGSKKTRANIKDYKTDLNNDAVCQLLALCAKLTKERLNGTESSDANGDDGDDQDENDNQDDQDQTEDEDQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V9C7</accession>
    <name>A0A8J4V9C7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000117</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000002">
        <property type="protein sequence ID" value="KAF2078617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V9C7"/>
    <dbReference type="OrthoDB" id="21512at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="259"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="380"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="394" mass="47572" checksum="97AEB00AF45B0D15" modified="2022-05-25" version="1">MDNKLKQDEILNHLELNKEYVKTILGGPQQQQRDDRDSSTGRDDHILASKSNLIPVGRNSSPSVSYLNNIIQQTVSHNNYLKENDELAYQHNKLNEPKRYNNNSRDSRDNRDNRDHRDHRDYYKRDRYNNDNNKRKQENKNNNHERSSIDIERERNAMEREKSYNRINSIGSTVIDEKEEKEREKQFNQYKESLKYNNSKNHGENQDKEFDDTIPDILKRYKVRGRGRIGSRVHDERKDKELLHKLQDKYGINLDVYRDNKPQDDYISDSDDSKTTTTRDYLYKRKSRSRSRSRSSESSSSDRKRNNRNKSSSSRSKRSKRRHKRSYSSSSSSRSRSRSRSRSTSSDSSEERREKRRRRERKKEKKRERKDRKEKKRRKERGESKDREKKSRDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VB76</accession>
    <name>A0A8J4VB76_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000787</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000016">
        <property type="protein sequence ID" value="KAF2077909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VB76"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="309" mass="35871" checksum="935779B6529D6CD1" modified="2022-05-25" version="1">MSSLSSSFELKISDLVPIQPHLKLELSSQALFIVLYIVKFIYLVKIEKKYHGFKLKLGVHIICFLFNFFNFFANLFLFISKTSVEQWFGYVHFVVLIMYMIVLASALLLFLHWVSMITDVFSVTKGGKILTRNNGILFFGVFFVPNLILSIFFYIVAIKYREKVNFDLLMKVQQYFLLASVCLVTFILWGFLVYQLNVMNTLNRNSAKKFFAMAFLFSLTTVLIPTLEIVIEFSPISDMRWCMVLSVVSMILFHFQILFSVFPISLYRIKKHYNLQFSDHSNPKNSNIPLRNAEELNSTSTSISVCSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJS0</accession>
    <name>A0A8J4PJS0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010509</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001159">
        <property type="protein sequence ID" value="KAF2068167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJS0"/>
    <dbReference type="OrthoDB" id="2594567at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045727">
        <property type="entry name" value="DUF6081"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19559">
        <property type="entry name" value="DUF6081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="136" mass="15537" checksum="E6502F9DE91A5A71" modified="2022-05-25" version="1" fragment="single">NVEEFHELTIVFSKPDKSIKWFVGQQQVFTVTKVGNHLPAAYQQYMFLDRGGDVQDVFPDDVNIAFGTFSLLDSTFPQSSPNKGLVRLGESLTEYRNPLYPEKSLDFFDEKSTISNRLFGQGSVFDIKDIKVYVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJY8</accession>
    <name>A0A8J4PJY8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carbohydrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010161</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000952">
        <property type="protein sequence ID" value="KAF2068514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJY8"/>
    <dbReference type="OrthoDB" id="17104at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019028">
        <property type="entry name" value="CBM_49"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052879">
        <property type="entry name" value="Dd_Spore_Germination_Stalk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33239:SF9">
        <property type="entry name" value="CARBOHYDRATE BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33239">
        <property type="entry name" value="CELLULOSE-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09478">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01063">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035188794" description="Carbohydrate binding domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate binding" evidence="2">
        <location>
            <begin position="52"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="150" mass="16471" checksum="4B89B8D6D0C39E3A" modified="2022-05-25" version="1">MKFITALLFALIAISISSAVADHQCGVHRCPDMHACVCEAGVFRCVFRCDDVIITQTVTRRWADNGGASPFAQVEVSVKNVGHRMVKDVTIGTVGMNLKDNNAIWGISVEDCDIHLPSYVDLAPHQTHTFGYINKGHDAAHLFVKNINLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKB2</accession>
    <name>A0A8J4PKB2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA dimethylallyltransferase</fullName>
            <ecNumber evidence="3">2.5.1.75</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_010358</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>adenosine(37) in tRNA + dimethylallyl diphosphate = N(6)-dimethylallyladenosine(37) in tRNA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:26482"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10375"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74415"/>
            <dbReference type="EC" id="2.5.1.75"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the IPP transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.75" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01001060">
        <property type="protein sequence ID" value="KAF2068315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKB2"/>
    <dbReference type="OrthoDB" id="775260at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052381">
        <property type="term" value="F:tRNA dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00185">
        <property type="entry name" value="IPP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039657">
        <property type="entry name" value="Dimethylallyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018022">
        <property type="entry name" value="IPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00174">
        <property type="entry name" value="miaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088:SF60">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01715">
        <property type="entry name" value="IPPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="364"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2068315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="476" mass="55771" checksum="251AA496503DEB8D" modified="2022-05-25" version="1">MYFVRRFCSSTTTTTTITQNLVNNAARDTLPKVLVIAGPTGIGKTEISIKVAEKLNGEIICADSIQVYKNLQIGSNKITVQQQKQIKHHMFDILDVNEEHFNAQKYFIQTVDTIKDIVSRGKTPIIVGGCGFYIECVVKGLHDQVSLTNEERDEIKKIEIKLKEKENWIDALNKLKELDPESANRLVKNDYYRLAKSLYINSVKGERYSSYIQEKQLSIADQYDFRSFYLTAPRTVLMEILNQRSEKMIAQGIIQETVEMLMKGWNPFSRSSQSIGYRETIDILLQPPTRIIDDEKTAQFIRDFQSATRIYSRRQQTWFKRDANMEWIIANPLPNFEPVPPPKLSKRQLKKANNPLFRKEILLKQEEYNRIEREERENKRLKEENTISSVDHIIKSFNQSYSIFQMNRNREEEDKLKVYSKQESKTYPQKFRIFETPDLRKNLVKALNSARAYLSRNKPELFTMKNPFTKTFNPDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKF6</accession>
    <name>A0A8J4PKF6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000912">
        <property type="protein sequence ID" value="KAF2068602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKF6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053331">
        <property type="entry name" value="EGF-like_comC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24032">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="83" mass="9034" checksum="C1233A4B0230D246" modified="2022-05-25" version="1" fragment="single">ILDPDFSILISNKDQNGCKSTTTSNRDWVVPTAVVVSIVGAAIIGTIIAVISSRFYYIKISRKGVIFIKKEKKKSGTDGIQMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLJ6</accession>
    <name>A0A8J4PLJ6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008524</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000533">
        <property type="protein sequence ID" value="KAF2070152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLJ6"/>
    <dbReference type="OrthoDB" id="21419at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="29"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="211"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="31"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="45"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="83"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="361" mass="40853" checksum="49782B8A985637A6" modified="2022-05-25" version="1">MDILSNPNSIPMNTLDRIIKETSSSNDLKENTNINTNNNNNINNGSENTIYYQPNLNSLPSDQDNFDNTNYKIPTGSSDYFNLSSSQMMQIQQHQQRLQENNNNNNTNNNENNENNEATNTPNTPASPSTTDEFRTIHLRQIQERIQAETLSSNYTKFNIIYGSMGIVLVLATIILFIVGFMKQSQTIIFIGIVASPIGFVFINHSIHIAYRRSRRHIEMLQQQYRQIQDNAEQTVVDMANVPSKLQELSIDILSSPSMFEKKINQDSFYETENNSDYKPLDSTIASSFTTITDSCCITNAVSMASSISTLDDQSNVLYSQSQQSENYLIYSPKPTSSNNIETTAIDKHYQQSQQPHQSCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLU3</accession>
    <name>A0A8J4PLU3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Annexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_009725</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="domain">
        <text evidence="6">A pair of annexin repeats may form one binding site for calcium and phospholipid.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the annexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000788">
        <property type="protein sequence ID" value="KAF2068959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLU3"/>
    <dbReference type="OrthoDB" id="16758at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000002">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000004">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000005">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.10">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001464">
        <property type="entry name" value="Annexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018502">
        <property type="entry name" value="Annexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018252">
        <property type="entry name" value="Annexin_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037104">
        <property type="entry name" value="Annexin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502">
        <property type="entry name" value="ANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502:SF102">
        <property type="entry name" value="ANNEXIN B11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00191">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00196">
        <property type="entry name" value="ANNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00335">
        <property type="entry name" value="ANX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47874">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00223">
        <property type="entry name" value="ANNEXIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51897">
        <property type="entry name" value="ANNEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0041">Annexin</keyword>
    <keyword evidence="3 6" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 6" id="KW-0111">Calcium/phospholipid-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2068959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="403" mass="44834" checksum="DFFD8CA86D035D95" modified="2022-05-25" version="1" fragment="single">TSTRLPTTTRLPPQQGYPPQQGYPPQQGYPPQQGYPPQQGYPPQQGYPPQQGYPPAAGVPQAYPPAGGVPLTGYPPLGAGVIGHAPVPGYFEGYYIGFITHDTKKDAEVLRKAMKGIGTDESAIIKVLANRCWAERDQIKKDFSAMYGKDLIKEIKSETSGNFEKTLVALLTEPSLYDAEILYEGMKGAGTNENALMEILITRNNAQLFKINQSFQRLSSKSLKDWVKKETSGDFKDLMEYLLEPRDESNIVDNALVIKDAEDLYQAGEGKIGTNEKVFTRILGKRSFVHLNLVAQEYERRHKKHSLEKAIDSEFSGNLRTALIGIVIYARSCFEYFAKILNKSMAGAGTNDDKLIRTIVTQMHLIEGIARSYNTIYGKTLTHAINTDCSGDYKKLLLDIVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLW2</accession>
    <name>A0A8J4PLW2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009330</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000681">
        <property type="protein sequence ID" value="KAF2069348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLW2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="811" mass="94960" checksum="23BDA58A66BD47E4" modified="2022-05-25" version="1">MDIIFFQIFRNLFLRNKIFSNLQQYKTKHNIYNNGVLNHAYDDLQFIDLINTNNSHLIKEKIHRYKEIFNSNNSDNNSKSNSSFYDDWLDIPFDILFKSSTFKSLAERDKDLFISVLKDLMNTGLFPSMRGRVERFHEYVSQNIDIDKEVFELLLQGHLLQMLEDSSDGKIVSRSQVEKLIQYKYPLGRIVDARLRLDLLVDKRIEFSYRELVDNEFFFAHKDQFRGLEKWSDIEFIESLLSGGLDSGTNAKELFVWMLGVSPLVNYALFIAQNPDKASYTPRFTLFGVEIYVNRRDNFFRNVHFWFRVPEVTEIILKTYGAGFQKSVYTGCRDLYIDSLEIAHMLVDFLLANPAFYQDRLLLHVFSSSTDLALYKLFWSHNILVYGVSHLSFADLQEFTGLLEIDKHSTPPQIKLAKEQFKELYRERNIFTETTVDFLLSVIKTQYCTQFPLPNDMIEHAMVQAINFRRAKYIVFFGALINYRSLWDSGYRILAELYRTNQFTSLFDQISWTPPLDEDSNVFLRMCNVCIQSHDPAMTKFFYEHMDSNSAKRNIPDLLVESYLRASQTKTPSDKQKAWAIIDYLRSEAETWNVGLDPLRKGFVLVQDPQFGTIPLDTTRLLLSHALVEGTLRAAKTILDSLTDQQLEEIDFHALLIQYFVRHTPMLKVPLYLIQRFQHRFTITFPIELSCIAYKSGNQQLVDILLNKTKIRLLPQLNQNDIVSNVYMNLCKLKQRYTSPSNSLNIGDISTDPQTLENFTMVDVQLEKNQWYSKTSHNINCTTYYNESNYYQDFKPSSSTRSKLINGLLFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLY6</accession>
    <name>A0A8J4PLY6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_008248</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000492">
        <property type="protein sequence ID" value="KAF2070437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLY6"/>
    <dbReference type="OrthoDB" id="18420at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000286">
        <property type="entry name" value="MFS efflux transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23121">
        <property type="entry name" value="SODIUM-DEPENDENT GLUCOSE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23121:SF9">
        <property type="entry name" value="SODIUM-DEPENDENT GLUCOSE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="214"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="280"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="305"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="341"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="413"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="456"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="481"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="504"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="538"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="568"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="64"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="90"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2070437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="617" mass="68518" checksum="E3EAEA842E8A9A04" modified="2022-05-25" version="1">MVHIYENDNNNSNDDEDSSILAVQQQEEQENNSSGNNTTLATTAAEVETIKTTTNNTDTIKDINDHAILTNEENIEEEEDDGDDKDRLISNHNQNSSVSNSSSSNSSISSTPRSLNNNNNKSHSIDKDEVELTTYHISKNGEMRPTTITTNHHESGLVVDYHSDLNEEDFISVNDSGYFKPFGSRFLEKNIYYEKILNVSNKLRVDATIHKSELILTAAFLFCFISLGISVGSLGPTLILLKDNTNSTLDMVGYFFTARGLGYFIGSFASRVYDRVNGNFLVAGSVLFMSIVLFLIPLLKNVWLAGGLFFFEGIFAGLIDCGLNTMIVWVWKEKVNPFMQLLHFSFGVGAFLAPLVVSQMVGKTLFFQYTILAAIMFVPALTIVLLKPAKPFHHEEMDGAPVSKIERTLRLQVIVAMAFFLFFYVGSEMGYGAWVFTYSKLNLSLSDESAALLNSLFWGSFTFGRLAGVFVSLFLSPQQMVITDTLGCFFFAILLILFPSSQTILWISTAGLGISLASIFPTAFSLPNNLNMPVTSRTTSYMVIGATVGELSIPWATGVLQHHIGMHVLPWIVFLSLLVSLTIYIGVNLQTVRMRSKDRGHSFNLSWKSVVSLLENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM61</accession>
    <name>A0A8J4PM61_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tetratricopeptide-like helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009260</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000665">
        <property type="protein sequence ID" value="KAF2069425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM61"/>
    <dbReference type="OrthoDB" id="1926212at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="527" mass="60292" checksum="3D0D1C741502BF1A" modified="2022-05-25" version="1">MIRSSIGRLSITTHTYLSLRPLTQPLVKCNNINKEKSSFAIQSLSPCCSTLCKGINDNDKRYFITSSQPRLFFGFFSKKKVETDNRSYRQLVDCAVDNIKNVNYDVAMKELERAQSLSSDVPDAYLLQADILQHIYHNYKGAIKLYDHCLKLDNVKQNKQLYSSITNSMIECYSYLSDPVNAFKWISALDTKDLNASALYQLFKSCLIKNSMNDVVRLLDLIKDVDYNTYLLASGDAKQNDVKGAIQYYNQVYENEKEELKKSKHVDKDIYITDRDGCYRFIVSKRKEILFNAMIGLARCYIGTNEPAKAYQLYKEASTLNFDAVFTFSKMAEIMVHLKNYKQALLDINQSLLANPKKQSTNLLKAEILYHLGEYKQCADIICPILLYSFQTDLPPTEIAPRTRYIEIAIESLLHIIQQTPSNVNDKLAITLDEKNERVLDTQAKDLQQLSNQAIVLQTALIKQIKQMKNDAQLTSLVQMRLSCMTFITRGIMETSILHSTGKKPLFTNPDLLFTSIKKLACILLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM74</accession>
    <name>A0A8J4PM74_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_009573</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000746">
        <property type="protein sequence ID" value="KAF2069109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM74"/>
    <dbReference type="OrthoDB" id="416741at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00048">
        <property type="entry name" value="DSRM_SF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051363">
        <property type="entry name" value="RLR_Helicase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14074:SF16">
        <property type="entry name" value="ANTIVIRAL INNATE IMMUNE RESPONSE RECEPTOR RIG-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14074">
        <property type="entry name" value="HELICASE WITH DEATH DOMAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DRBM" evidence="3">
        <location>
            <begin position="2"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="4">
        <location>
            <begin position="295"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="5">
        <location>
            <begin position="612"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="3">
        <location>
            <begin position="792"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="72"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="156"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="74"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="161"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2069109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="883" mass="98503" checksum="7F28C0775E4015D3" modified="2022-05-25" version="1">MNYKGLLQEYLVKQKSPNPKYTTIERGLDHCKEFQSTVHIGNQSFQSNWCLSKKDAEKNCAEAALAHFNIVKKPSSSSSSSSPVSPNSHTKTPYSRPDGLHSGGGSGGSNLTSLLSSTMKKSNGANVNIRDSNGSVSHYQTTTLLNSSTTTYDYDHQHNDTSFSPSSSPTSSIGGFSSLGPTISSQETTMLTFINELSKLKIQDPNNEPSQQSEPLSPSQMNDIMGLLKVNPHSALPMLEKLLNDFSGYISNIKESVPKFNYQESINTPVKCIDDDPAFIPDMSYNTPRDYQIEMYNKSMKENIICCLPTGLGKTMISCMTIKRMKELNPKKRILFLTDRIPLVLQQSQVIKNETGLRTIAIHGDNFKSEMLSQEYDVMVVISALLNNLIFSKKVDLNTFSLIVIDEAHHIVKSHPFAVILKQYYKHMKQEMRPKILGLTASPAGKSDFLNTLISLKLISDISHCKLVHADERMLQPFLNNKNIEIIDIETNSIEELTLKYIKDTLSLFNIPTSSDNSLDYLCNNGDQSLSSSTNSTSSNIRSTEEAKSFILKVAERLINYRDFGIRESIKSTKQLICEELVNLQDGPIKAMLVKLWSMVPDTEESAQNISKLDKALDILKNFKARQGSDFRCILFVKTREMANLLNELLIDFSSKSLEYSFIKPALVIGHGVGGDGMSVNKQKINIEKFRTGECNIIVATSVVEEGFDVPSCNLVIRFDPPTTVTAMIQSRGRLREKDSHFIAIIKKNVENSGKYDLFKMQELYLRDTLLFLTNPNCPLPATAEIAPVVGNSIAAVQEIVQKLQSTFPSRAVEVDWAFTQIAGDAHNPTFQCKATVLAVRPKTNERITIQHIDTGSSKQDSKERAANKLLTSNHLLHLIRDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMQ1</accession>
    <name>A0A8J4PMQ1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Arylamine N-acetyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009525</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arylamine N-acetyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000731">
        <property type="protein sequence ID" value="KAF2069156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMQ1"/>
    <dbReference type="OrthoDB" id="17550at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2140.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001447">
        <property type="entry name" value="Arylamine_N-AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053710">
        <property type="entry name" value="Arylamine_NAT_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786:SF0">
        <property type="entry name" value="ARYLAMINE N-ACETYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786">
        <property type="entry name" value="N-HYDROXYARYLAMINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00797">
        <property type="entry name" value="Acetyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="293" mass="33724" checksum="4A1362FD3EA8784D" modified="2022-05-25" version="1">MDFLDLFLKHCNLPKNFRCNNLNDVSTAMKAFQVTSPYNNLTIFDSSWEDKTFESITRKVLIEKTAGCCIELNSTFNHFLKLNGLDTKLVRVLHSDKNNHENLRHHYFTILKYKNIQYTVDVASGVASPLYPLRIDAQSDSDVVVGLNGLLFRSISNNDGSFLFQGNSSTSINEIKSHCWLNIYHYTLEPVVGYSQLSESQILGGLLHPQFNKGPMLNILTADENLNITGILTLTEKTLTENKPGNEKTKIPIKDNEMYNYYLKTRFNYQKYFQLKPSYYDSLNKRGHFKSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMU0</accession>
    <name>A0A8J4PMU0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009448</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000711">
        <property type="protein sequence ID" value="KAF2069231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMU0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="11989" checksum="28F643247DCA71FC" modified="2022-05-25" version="1">MPWGTNYFAAGTADNFIHIYDLKRSFYYSKKLTGHVFQTKGLALCTQLNDKYLYSASLDKQSILWNVEKGFIVSVFPLGSPTSSFTKTTTMISLLSYQNILVKSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN38</accession>
    <name>A0A8J4PN38_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carbohydrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000505">
        <property type="protein sequence ID" value="KAF2070338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN38"/>
    <dbReference type="OrthoDB" id="10393887at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38746">
        <property type="entry name" value="CBM49 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035144817" description="Carbohydrate binding domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="167" mass="18383" checksum="9986459548E7A55E" modified="2022-05-25" version="1">MKFIILSLFIILSLSLMINGQTTSSQSPATVTPTSTFESYFQNSNLQVQMTIVSPTGEVLYGKNQLTLYYSDFVNGNSLPLVSSVAETCNKNKTQPFDASSAKVSFNDRNGIIVKKSGIVTYNPVWYTSGFQFQLIKNGNIYYAFSNGYLFTFVFNTTTGVTGSACK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNP2</accession>
    <name>A0A8J4PNP2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nucleoporin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000491">
        <property type="protein sequence ID" value="KAF2070445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNP2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="600"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="600"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="632"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="672"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="738"/>
            <end position="749"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="749" mass="81530" checksum="AACF92E44718A517" modified="2022-05-25" version="1">MLNIDEIKKVKIFAAHSTAKITREYSLFEFNWNKSIIIDIPEKEFQSEQGIKVTSNEFQFEQLKSPIISQRIFSKDVNDVINFTKSHSTEILLKFQLTDGSIVIGTIIREISSSASMGFHDKGASFIIQVDNHILPPNSKINEQKGFITIPKSYILSVTPLDSDQIRGTKVQLFLAPKNGNTGTLSYTTDDGSNVVGSNNNNNSPLCGRKILVTYYISIPSGITVQRKIKFNNKDIDQLVENPSLKAIHFKIDTIVSWTSLWNLHQLPIKVVYCGLEKQLSPYTSIDVGESVNITIDSLSTQGDRVIYCTLGAKLLKPAILFTNETQEIIQAGSLETGPTTTSLISRLYDKKSAFGTPLSSQTYSIPNSIPSGSQGVILIENYSQKSIDKSFSLEYNINNYPNNHTIKSMKVSDLLTIFEMSAKNLHQFTFTNISNQSTKLLISVNSAASEEEKYKSKDLPLITSGSTNIFTKEIDPLSITKFSFSHVKYSTSFSRTIFPTMLDEFLALNGIHRRPSQSKFEIIDLKSLKKTSAQALKQLVFANFYIFNSEGKVIMNNTNFKTNTGFGQGQHQTSAFAFGSAPQPQQSSLFGGISSVPQQQQSSLFGGSSSFGSGANSNSPFAPQQSGFGGVSSAPQQQQQFGFGGVSSAPQQQQQQQQPSPGTTSTGLFGAPVSSNIAPSANSSAFGQTTGGCFGSQAFAPQQAPLTGTTTVPQPTPAPTFKFTASSQPSASPPAMPSFSNNSNDVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPR6</accession>
    <name>A0A8J4PPR6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Alpha-mannosidase</fullName>
            <ecNumber evidence="13">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_008891</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.</text>
            <dbReference type="EC" id="3.2.1.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the glycosyl hydrolase 38 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="13"/>
    <dbReference type="EMBL" id="AJWJ01000595">
        <property type="protein sequence ID" value="KAF2069784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPR6"/>
    <dbReference type="OrthoDB" id="2016903at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00451">
        <property type="entry name" value="GH38N_AMII_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000001">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.30:FF:000014">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.110.10:FF:000012">
        <property type="entry name" value="Alpha-mannosidase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.50">
        <property type="entry name" value="Glycoside hydrolase family 38, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041147">
        <property type="entry name" value="GH38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011682">
        <property type="entry name" value="Glyco_hydro_38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015341">
        <property type="entry name" value="Glyco_hydro_38_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037094">
        <property type="entry name" value="Glyco_hydro_38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028995">
        <property type="entry name" value="Glyco_hydro_57/38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050843">
        <property type="entry name" value="Glycosyl_Hydrlase_38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607">
        <property type="entry name" value="ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607:SF19">
        <property type="entry name" value="ALPHA-MANNOSIDASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09261">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17677">
        <property type="entry name" value="Glyco_hydro38C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07748">
        <property type="entry name" value="Glyco_hydro_38C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00872">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88688">
        <property type="entry name" value="Families 57/38 glycoside transferase middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 13" id="KW-0732">Signal</keyword>
    <keyword evidence="9 13" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035338870" description="Alpha-mannosidase" evidence="13">
        <location>
            <begin position="22"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 38 central" evidence="14">
        <location>
            <begin position="403"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2069784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1033" mass="118894" checksum="68E893E5D4F75E7B" modified="2022-05-25" version="1">MKLFLLIIVLLSIVFIQTTNTTSIHINNDLDIDINQQKQQRDININDDNNNNNNNINVFLIPHSHCDVGWVQTYEQYYSENVTVILNNVIDHLLKDSTKKFNWAEIIYFERWYNSQSSILQNQVRQLINNKQLEFIGGGWAQNDEAATHHEAIINQMTLGHQFLLSEFGVTPSVGWQIDPFGPSTFTATAFSLMSFKYHVINRIDDRLKHVYNNTPDIIGSGILTTDQEFEFIWYPSRNYGQQLSIFTHILDNHYNSPSICYPNVTNPNTTICTGFDFEADPSSNPPINETNIYERACFLVEIIQQRSALYRHNNLLLPFGNDFRFQNATLEFTNMDKLIDYINSNSSWGINIQYSTLSQYFDTVFQEIVNVNETFPEIQGQDYYTYTMCLAIDYQNFNTCVNYWSGYFTSYPLLKQTARDSDSLLRNAEILYSLASAYGNGFDFDFTPSYESLFYHRNVSGILTHHDAITGTAKAYVRENYFDMLYQAQNQTLKHLVPSLVEFLLANKSIPMNYTANINVLLELGQDDILAVSFTNSLAWDRQEFVYLEISNQQYIAVYNDKLESIESQIVNRIDKGGKWYLYFMVNTPALGISTYFIVGLSSTSPNSNQFLNEYSNIISKPILAFNSINENILEMSLPTTSTFSIGNSLFNLNFKFNSNNNNILRLESFDDLVRNINSIPLVQELIEYQAMSDDAYKFRPKGYPTTLVAETPQFYYTQGPIIQLITIIYNNNCSQVFSVFNSSSSSSSSFLNSNQYFEIDNVVAAGWDKEISMRFNTSINNGQTFYTNNGVEVLERNYQMKYNDSFPWSLISGNFYPVINTGYIVDQENQLNVLSKQSFAASSQDNGVFEYLLIRRCNYTQWSIHETMNDTSNPSLKFRILFGEPNSVQSIRSPHSVIFENPMLAVFSSSVQEIGLLKWISTFNTKFEPLVQSFPYNLHLVTLTKQWIDSSTNIIRLMNIYEINQNQQYSQPLDVDFNSCLISNFNISAVYETNLSANQIISNLNDNDDILATTLNPIQLKTFMFELNPTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPW0</accession>
    <name>A0A8J4PPW0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008019</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000462">
        <property type="protein sequence ID" value="KAF2070656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPW0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="628" mass="73253" checksum="77434257E0C1C337" modified="2022-05-25" version="1" fragment="single">QVLEYYYKPKDHDTDWSHQLNNIDVVKYLFDRGYGRDITSINLVRIDINLLEYYLENHWLDASILIFIEYKAELEISNKMSRIDLKDKIELIVKYIESIDPEDLSQLFENMIERPIPSLIKPLYHLLEKLDALHMANNVLQAESSMTLEQARNSVQEEEDRLVAFANSMSSNNDNNNNNISVQKYKCLTMIQEKLAARKSLWPTWINNQGQEFIKSWQQSSRIKVTTQNIYYSFLKDGKDHQTLSRHHKDIASIICMTCGMNVNVQAFRFMYQQGFTSPNINYLSYQELVRLTSDQDRDAIVELSNSPFVLNPDHDQNIQDPETRNILSKDEILSSCCEGGHDKNLDYYLGKFKAYLVDQEDINELFMDARQHRDAIKVLYSHGFTYTGMYDGEGLYNHCWFDKFSKDRVTTALRGLGPDLYQSIKGYLLDQLIEYIVIKNDYVSFKYLLDNNSDIKLNTTQDPPDEDLIGHLASSTNINIIDYINKNKSTCLSSDVDINSFFGSVFIKANTKFVFNAPFTEYLVTNNLITTDLYPFHPATLDSFGSKVFNLFHFIYFAHHYKYIDHQTNSSMTLVEFISRNQYKCHGELHFLSIYGEEGLDKHGFSNLLVDLTNTYDIGETKKYKRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ22</accession>
    <name>A0A8J4PQ22_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006612</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000313">
        <property type="protein sequence ID" value="KAF2072070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ22"/>
    <dbReference type="OrthoDB" id="24016at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="540" mass="60900" checksum="7D45B261DEE88F4E" modified="2022-05-25" version="1">MLNSVNHTGSNKRNMINAPAAPTTFASPNKPYQIIYRNTFLKNIVSQYVCNDLDLNVSFEYLNHHHQDLARLDTLNYNTQISVLILDDEQSGLYQESPYKDLITKMFISDAVGPINQSQIYQHLPKNLKSLEFTHTGREITYGENIKRLLIDYRSETPHGPYNSTTLPKGLKSLSVFGPSHPFTENVIPDSLTDLQIASDDLVLYNASKFPSTLTYVELNVMNTFPQRPVFNNQHFIKKLDISFADGVTLEPNDLPSTVESLSLGGNIGELRIKVLPQSITELELNDYAFQLKPNILPSHLKKLSLYVYDQPLLVGVLPQTLKELTLHLYDKPIGAKVLPSSLSLLRLPSFNQPLSPKVLPNITDLYLPTYCQPIEFGVFPDSVKTLLISHALPISDSCQFPSSLTRLEITNFDGSRGFLHLIPPQIKSLYIDFFVQGGEQKIEFDPQAQIPPHITSLLFGHNCFRIPQSFIPPTVKKLTLGSYETVIQPTVEGECFYPPHLELLSVTSDFKVGKIPDSLKIIHFNDCILQHIKREGDQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ23</accession>
    <name>A0A8J4PQ23_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_006777</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000328">
        <property type="protein sequence ID" value="KAF2071898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ23"/>
    <dbReference type="OrthoDB" id="273771at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16504">
        <property type="entry name" value="RING-HC_COP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000090">
        <property type="entry name" value="E3 ubiquitin-protein ligase RFWD2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042755">
        <property type="entry name" value="COP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44080">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE COP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44080:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE COP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="2 12" id="KW-0853">WD repeat</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="152"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="580"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="666"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="336"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="52"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="82"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="339"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2071898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="796" mass="88245" checksum="53968A1C9E1DA904" modified="2022-05-25" version="1">MSSISIIKNNNSSSNDSTNNGKSSSSSSTSTSTSLTTSPILSSSNKPKAKVTISSYNPNPVVNDSTAATLIVNGNNNSTPASNSESINGSDSSSSNNSSSNSNNSNNNNSNGNGNGNSSNIGNGSKGSDDNNNNNNSSKKSKDRLVDDSLLCPICLDIIKEPFITKCGHSFCYQCITPHLSKSNTCPMCMGFLTREQIIPNFALSKFVEKMTAQNNRFSIGPPAKKFRNSINNEDLSINEINIIVSTLLEKKKHLELQDQQVEYDVLLDFLCKTRQQKMEVFKQLKRQISYLDNDIKAVDYYKQISEKKNETSNSNNNNNNGLDLKMSSNEIETTNLKTSSQNINTSLSSSSTSTTTTTTTSTKKLNGSTTTTQPSQQNIIANKKKRVETHLEDLQSCYFSAYKEIESEQQQPLHNSIDNLDTNSLSFSSTSIDNNNNNAMINYSNGNVNHHIENVKMGKGLLNFSKNLLKFTRYNEFKVIATLKYGDLYNTSSIVSSIEFDRDDEFFATAGVTKKIKIFEYAQIRDNVEVHSPTREMTCRSKISCLSWNTYIKSQIASSDYEGIISLWDANTGQNVMLLEEHEKRVWSVDFSRADPTLLASGSDDTKVKLWSTSSDRAVTTIESKANICCVKFNPNSSNLIAFGSADHHIHYYDLRQHKEPLFIFKGHRKAVSYVKFINKDEIISASTDSTLKLWDVNQNDCIRTFTGHSNEKNFVGLTVNGDYICCGSENNGVYTYYKTLSKPIVTHKFGTNSGTGEETDDDGSQFVSSVCWKKDSNILLAANSQGNIKVLELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQD9</accession>
    <name>A0A8J4PQD9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GLTSCR protein conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007483</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000402">
        <property type="protein sequence ID" value="KAF2071197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQD9"/>
    <dbReference type="OrthoDB" id="21659at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="440"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="539"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="727"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="813"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1102"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1223"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="94"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="282"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="399"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="539"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="595"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="730"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="742"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="752"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1102"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1140"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1151"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1223"/>
            <end position="1266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1267"/>
            <end position="1278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1278" mass="144631" checksum="D176CBC26B5AF765" modified="2022-05-25" version="1">MSTSINGNGFIDDINSFHNEHINTSLDKNSDSNDNNNNNIGLSEMADSNIDGDIPSKQPMSDFINNSDNNDLNEYNNKNSNSNSNSNDDHHHSNNNNNNNSNSNSNSNNSSNSNSSNNHNNGDDLQQQFVNTFDNEKSPLGDQYLKDPYQEDSTWDTNKPDSPFLNSSIMSEDVNTTFSNTETSTGVSEQQQQESNDNNNNNSDNTRSNQFSGSSNENISLNSNPSNEDIKNSSFQQQQQQQHMNGTPLSNPTSPQTPMSPKQQQQQQQQQYVSTTQHMNYVSTPQQQQNVSTPQQTQQVTTPQQSQQVTTPQQSQQVSTPQASYNISTPQQQVSTPQQPQQQAQQVSTPQASYNNVSTPQQQSTPQQQTSPNQTSQPLSTVPSPIHNISQLVAQVTHQQSQNQQQNSQQSPQQQQPLNIQQQISQQQQQQPIQQQQQQHIQQQQQQQPIQQQSNQGHAQQQQQQQPTQQPQNQHSLQPQYNQTQQQYQQQQQFQHQLLQQQQQQQQLFSYQNPQLYQQQQQQQQQYQQQQQQLQPQQQQPQQQQQQQQPQQQPHNIQQIQPQQQPQPQQPQAQTPQQPPTQRYITPQQQPPPSSSNVPIQQQPQPQPQQPQSQSQSQSQQPPQPQQQIHPTQLAMAINQGQQALINIENQQKLVRDQLNTLQKQNMQYQTLIAQQPQQQAQIQPQLQQLAERHQGLVSQFNNLSQLHTKYKQQLIYFQSQYQQQQAMAGGNGNNNSGGGVNNSNGQQPNSTIFAQPQSNSTNNNSGGVQQPSNPGQPNNAYLKLHPQQVIQMNKAGQPISLSSTQAHQVQQIQNQLQQQQHAHAQQQHQLLQQQQQQQQNQHQHQQNQQPQLIIQQSQSTLDDENKKKRKAEKVLEKKGDLKRIDDEYKKKLEELTAERKLATDPDYKTPFKSIEDVKQRLNCFRVFSVLDTTLSKQESDNLLNDTLNNVNKFFENIKKVVEQQSKVPNLFYREDILQLDEVFTKDIEFKHRYNALQNGSITSSITSPIVNSSSMTPTSVTPIFSSSPISNLSSPNALVAKQQHQQHQQLYAQYHLGNLQKPIPPQPASTLAAAQAIVAQHQQQQQQLLLQQQQQQLQAQSTTPSITTPTSIVINSPLNSSSSNSISTPITVSNTPSNMFTPPSHTPQLTPNISSSTTTTNNQQQPQPQLYPQQVISPLNTSSFSTVSPTPFNVNNNFNQNNVAKPSLLHPNQQQQQFSLNNQNNSANNNIGSEINNNNNNNNNNNNNNNNNNNNNNNNNNNNNNITPSLMQSSPQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQY0</accession>
    <name>A0A8J4PQY0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">OPA3-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_006185</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OPA3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000274">
        <property type="protein sequence ID" value="KAF2072498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQY0"/>
    <dbReference type="OrthoDB" id="2129069at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019216">
        <property type="term" value="P:regulation of lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010754">
        <property type="entry name" value="OPA3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12499:SF0">
        <property type="entry name" value="OPTIC ATROPHY 3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12499">
        <property type="entry name" value="OPTIC ATROPHY 3 PROTEIN OPA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07047">
        <property type="entry name" value="OPA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2072498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="20859" checksum="66EC9EDC80B32293" modified="2022-05-25" version="1">MVLPLLKVGSLVIKSLAKPFSKQIKFAAAKSPIFHANIISMARLWYKVDLKLSKFSGDTTRKPLELNVNAAIDLGAEMLSEAFLLAVALSLLVLENKRSSAKDQKKEEALAQKFTDLENIIENQNASIKELQDALNAVNPNLHLTLHFNDKNNPSKSTTSTLSNIPLQLQTANNNNNNNPANTSPNSIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR08</accession>
    <name>A0A8J4PR08_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carbohydrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006657</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000317">
        <property type="protein sequence ID" value="KAF2072023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR08"/>
    <dbReference type="OrthoDB" id="10517104at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019028">
        <property type="entry name" value="CBM_49"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052879">
        <property type="entry name" value="Dd_Spore_Germination_Stalk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33239:SF11">
        <property type="entry name" value="CARBOHYDRATE BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33239">
        <property type="entry name" value="CELLULOSE-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09478">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01063">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035249473" description="Carbohydrate binding domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate binding" evidence="2">
        <location>
            <begin position="63"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="163" mass="18815" checksum="4E8381D06404D962" modified="2022-05-25" version="1">MRFHSRIFFMVVLLVVCLGIKKSWESNQADNCIQYPNICKAHETCVASGDEKYFACEPNKEKIQLRKHLIKSYEDGQNSKYYEYDITLINNSNNEIKELRLCVDDAALNLKDVKAIWDIDRFPNGDLILPMGKHISPGSLYKFGFVARSELSESKFRVKSIVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR40</accession>
    <name>A0A8J4PR40_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007968</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000455">
        <property type="protein sequence ID" value="KAF2070714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR40"/>
    <dbReference type="OrthoDB" id="441660at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="270"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="339"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="388"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="416"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="448"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="32"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="497" mass="56632" checksum="0792E73C7034D2B3" modified="2022-05-25" version="1">MNNDNFNNNNSTDREKKEEGIDTTNSEIIVHIDSSSSSSSSSNSGNNNSSDSGGGINSSNLDLIPITTISNSNSISNIIDPSNSTYQIEQTSMIENDDDPFEHHTYTTTELAVSHQSHFDADDEGYDDNNRGIGDVNRVTTSYSTESIVSATAEDEYDEDEGVHVQEKQRLLFGTSRNPVYATDESSSGDEYLHKTKRIGSIVTKDVVVERTTTVSVSQAAFRKRVRHVLVMDAVLLAMFIPIVYFVYFIPSTYLGDEDSLRFWYYHWQWSKFVLWMLSIIIGFAYVAMITEQRLYPFHCRYARNRDFVQLLVASVLLFIIAASRTLMGPDDLNKHTPLSAFGYNYFFFASFLLGLHYTYLDKIMFLRAVGDKFGTPSLWYTYKLKEICAIVPIILFILSLVVWHCYLIIDEHLWSYYLIAYGGFLTLLIGVALLIRKTHYLHMHHYFIFGSLIPLSAFQTILSVFSQGVITGIMVEGVSRWSMGWLFYRGVRVLPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR62</accession>
    <name>A0A8J4PR62_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_006547</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Non-catalytic component of the proteasome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000308">
        <property type="protein sequence ID" value="KAF2072128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR62"/>
    <dbReference type="OrthoDB" id="10248542at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03760">
        <property type="entry name" value="proteasome_beta_type_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000074">
        <property type="entry name" value="Proteasome subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016295">
        <property type="entry name" value="Proteasome_beta4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF6">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001213">
        <property type="entry name" value="Psome_endopept_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR001213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2072128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="259" mass="28829" checksum="911053DE3EE2DCA1" modified="2022-05-25" version="1">MQQHVSFPVFGQNDYFNYKNPQTLENRMPVVDAKKKTQDPIVTGTSVIAIKYNGGVVMGTDMLCSYGSLARFNSIQRMEKFGSYTIIGAGGELSDFQSLSNTLNDLVTLDHCIDDGSKLSPSEIYNYVARVMYNHRNKGDPLWNSLVVMGYQNGSSFLGKVDLVGTCFKDDIVCTGYGAHIALPLMRKAALDNPNMNLEQAKKLIEDCLRVLFYRDARSSKKIQISVANENGLEISEPIELDTYMWQSGEAAVKSFSQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR79</accession>
    <name>A0A8J4PR79_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007140</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000364">
        <property type="protein sequence ID" value="KAF2071541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR79"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="143"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="227" mass="25144" checksum="CD87B42CE82664A3" modified="2022-05-25" version="1">MPSFEIERTYGVGGCFSYNYPLELNNLLGVEEYNEIITELNKSQSKKGVYITWTLFVLTILFVVIITYFTTSFLVMGIAIVAVVVIYSAATIGFYASMRSRVNRTVTRFNKEYHNRGIQFSASTTRVQRETYSTVRVTYNSSRSEVSSSPTPVSHNQGCYQPSVLPPPTVPSPPASPPPQPYNPAYSPPHPSSQVPVFQPMPSPSAPNLAIPVGPPPNECIRIDIDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRB2</accession>
    <name>A0A8J4PRB2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zeta toxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006463</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000299">
        <property type="protein sequence ID" value="KAF2072218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRB2"/>
    <dbReference type="OrthoDB" id="10263927at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33477">
        <property type="entry name" value="P-LOOP NTPASE DOMAIN-CONTAINING PROTEIN LPA1 HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33477:SF3">
        <property type="entry name" value="P-LOOP NTPASE DOMAIN-CONTAINING PROTEIN LPA1 HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="483"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="488"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="565"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="576"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="614"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="635"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="658"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="681" mass="76815" checksum="B0551F2A0181EA76" modified="2022-05-25" version="1">MSLRTDSPTSLISTGISSLQLNTSTNTSINNTNMNGITTSLNNNNSNNNIVDCIKEGTLAYIYLVDDSVFDNQQQQTIPLASKIYTPSPNKLPPFQFHQKSQDISILKDLYEYIKFFEIKDSKDFHTKDGIKRYARFHLNLTLKFMGCKSIHARQISNSVFEQFEKCRLEVYRKQQATTTTTTTNVNDTNTIYCVSIQRNIFYYVIGHILSCYQYSKPQYISDFPVASEVQDKKHSFTILLGGTSGCGKSTLTALLASRIGFTAVISTDNIRQLLRKFINRQESPILWASTYHAGEIISDPSLSHKEKILQGYEAQNEMIFNKLDVLIEHYEKRKESLIVEGVHLDTRLIVRLVKKHPSCIPFLMYISNEAKHKERFAIRSKYMTLDPHQNKYTKYFKNIRIINDHLCHGADEYMIPQIDNTSIDRSLATIHGTIFACLKRKVQFGESYFNHDTNKLNMLYNQYEQIQHQFWSSKGMLRLISQRKKSSSNNQNNSNSSNNNGGGNQINNNISSGSSSSSGSSSGSSSSSSSPSSLSSSANTLNSVSSSNNQNGVLNLSNNSNSNNHHHHHHHHKDVWKDSLDISNENDKRKEDEDEEEPSVIEPEIGGVGVGVGEEEQFNSDDDDGEEDISLGPHNKKLSKLKTHSREKSGNTNTSDLDSDDSDSEDERIDYFLDCGSLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRJ5</accession>
    <name>A0A8J4PRJ5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Heat shock protein DnaJ family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_007675</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000423">
        <property type="protein sequence ID" value="KAF2070999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRJ5"/>
    <dbReference type="OrthoDB" id="550424at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000048">
        <property type="entry name" value="DnaJ family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000001">
        <property type="entry name" value="DnaJ subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000003">
        <property type="entry name" value="DnaJ subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044713">
        <property type="entry name" value="DNJA1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43888">
        <property type="entry name" value="DNAJ-LIKE-2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="5"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="8">
        <location>
            <begin position="132"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="5">
        <location>
            <begin position="132"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="362"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2070999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="420" mass="46455" checksum="1E90DE6456944F9E" modified="2022-05-25" version="1">MPDNKYYDILGVARDASETDIKKAYRKLAIKYHPDKNQDPAAVDKFKEITVAYEVLTDSEKRGIYDKYGEEGLQQNGGGPGFSPDDIFSQFFGGGGFGGGFGGFGGRGGRRERKGEPLQHVLKVSLEDLYKGKISKLALQKNSKCQECDGKGANSKSADAIKKCDDCHGNGIKVQLRSIGPGMVQKLQTQCPTCKGEGNIIREKDRCVKCKGNKTVQEKKTLEVNIDKGMKHAQKIVFSEEGDYESPDIVPGDVIVVLQQKEHPVFTREGDDLVMDHKITLLEALTGFTFYIQHLDGRVLVVKSKPSEILKPGDIKCIYNEGMPGYKRPFEKGRLFIRFDVQFPANGSITPENAKLLEKILPKPKQTPKPVSHDGIDEEVELHAFDPNSKQGHSHSHSRASYDEDDDEGGHPQGVSCAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSG8</accession>
    <name>A0A8J4PSG8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004986</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000188">
        <property type="protein sequence ID" value="KAF2073698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSG8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="87" mass="10044" checksum="D6191DA62986D331" modified="2022-05-25" version="1">MTIIDNLMKLGQALEPHSSHDFHKMATSYAMEFRTQIRGQFCSNTILNTDYLMKTSYMFGYDNDYSFENETSTSNKKPKSNIGIKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSS1</accession>
    <name>A0A8J4PSS1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase M66 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_004802</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000177">
        <property type="protein sequence ID" value="KAF2073878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSS1"/>
    <dbReference type="OrthoDB" id="19788at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051256">
        <property type="entry name" value="Dictomallein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019503">
        <property type="entry name" value="Peptidase_M66_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39540:SF1">
        <property type="entry name" value="DICTOMALLEIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10462">
        <property type="entry name" value="Peptidase_M66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51694">
        <property type="entry name" value="PEPTIDASE_M66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035168379" description="Peptidase M66 domain-containing protein" evidence="10">
        <location>
            <begin position="21"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M66" evidence="11">
        <location>
            <begin position="143"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="623"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="630"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2073878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="677" mass="73075" checksum="01729D86C74857D9" modified="2022-05-25" version="1">MYKFIYLFIFLIAALSFANGAKLVISKFDGGQTHVIPAITGLEWKIKSVQTLHLVGKRDTLFVIEFSGGLTDLVSPKIEGLDGSTSLGSLVLNDPSKLPVTESNGTAYSTKAYSVLAPAKWLVPNVKVRVVASNYDASDYVTLNVGADARMIMYNLPFYLFGANDTNTMDFSKISTAPEAVIDELYNKWPVSVLDFVNHPAKRVQWPHIILNPNGTHPAIRATNKDFQRDGYEFMGHVLGILSEIRGINGDSGTNNQIYSPILWLNSKGVSSGVGGGLGGGHRGTGDASYSGIFIHEQGHAFGLPHAGEAYRANVSESSYPYVNGSLLGSAWGFDKTKNQFLGNFIPTEANSYKNCNKSYVLDGKNVCMKQTVMQGGSGCQAKGYTFSMFADYEAGVMQRYFEGVTTNGTNGNSYSGGRIVVTKSFPSGYMRWDSLDNKWVEFVPKTESSGLYGVNSSLPIKRDVNVYSMIASVSVARTPGATVLYPPLFYKGNLMPYIDPTDPEQLKTITPNKGPLAWYCHASDCGLTIRAAYPDGTVNHYLLQSGFKGWFSIYSNISADVFDPLKSASQRVVSINLPADKGPPSRYQILFTENGFKGIFPNATILLDYSCTGSGASIQCSEYVPPPTTTSTTGGSSTPDGGNSGTTGSDSEPNTSSLLTSNLVFVLISLISLLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSU4</accession>
    <name>A0A8J4PSU4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_005475</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000220">
        <property type="protein sequence ID" value="KAF2073193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSU4"/>
    <dbReference type="OrthoDB" id="5972258at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="3">
        <location>
            <begin position="5"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="869"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="170"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="426"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="489"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="880"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2073193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="934" mass="110560" checksum="81F6C89660C2B710" modified="2022-05-25" version="1" fragment="single">MTSIKKDKENLLKWCQNIIKPLGDKVSEIQDFANSWKDGSGFLAIVSLVFPDTFDYKEKLDTLTTPFDRVSYAFSVAEEKGIGSLVEQELFKQENESLNLENEKLSIETYLSCFYHYHIKKIENDKIDLKLKELLSNIDQISTSSSLDTTGAEDDSSATTGINSSNGWSMDKEIEKIESFKNKIKSINESNKSVELKRNQSAQTKQKLVDLESIISNIQKELIENENISKKFKEEIEQKELDLKNKIEKEKEEYERHIIELQKKISNLQSNSKEREELKLKLDLEKKAKEELLKKKQQYENDIAQLKSKLQQEQIEKEKLEQENNKMIQEKSIIETKHREDMKKLREELYNANLKNDILQMELNSEITLKDDLIKNKKEISDRVVDIQKNIDSELKEKAEIQKAKDKISASLKKSLLELEEETKIYNLKDKTKKEMEREKEKLAQLKQEKHLEKISVEKEKRIVENDLNEKRDSYDAELGKVREIKFNIRDIQQKIDSTEYDIEDEKERTTKLVAYFEEKAKVGQDRLNQKHSNILKKLEESKQQIKKKHLDTSDQLFKEIEKKKSLDKDKIDIQSKDKDLLEQYQKERKERRETEKVKRGLDDQLNQAKQFLALEGKGRSSREKVKSKLEMDTKEMKSNLSDTHLKKQSALDKKESLISDAINSEKKLSQSKHRVDTSIESRNNLKQENLDLDKDIVKLVQEKEAIKTDQEIKMMNNVEQLQKELKTQRNQLEQSKLELENASFKAQQDLLEKQQAIENMDNIKIEHEKNLKKLNDQLEQLDKEEMDHLKQKKIKEEIKSLRAQIDQDDQDKQEYSNQLIVLNQDVKRLEMELISESSESSLYSRQVEILQKRLVEAEDEMHQYKLDIEKQQNEQSHLAQKEKRRLEKEKSRQEKEIREELEKEHQRKLSQLELKFNRQKLELDEDELELEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSX6</accession>
    <name>A0A8J4PSX6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_005497</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000221">
        <property type="protein sequence ID" value="KAF2073180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSX6"/>
    <dbReference type="OrthoDB" id="1932312at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="367"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2073180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="46408" checksum="474D3EBE11F9E1A6" modified="2022-05-25" version="1">MFTSEIDPELELTTLIFQWLSERGYSQTLERLEAESAITYNPELVQFGSQLESVYNEHKHIQMSMKLAEEEVEDMTDAELNELGDSRFPQHLLDTLDKVHTSNILCMRFSPFDDSHIVVTGSTDKTIKVTNYLTKEVLYTFIGVSDAPMISLDFNPVNPELLLASSVDGSCSILRVPKDGVNCECIETYRQHKKYVVRARWSPDGQSFATCSYDKSIAHYKKDDQGKYSLVKVWDFPNTIESLIYTPDSKYIVAGVRESNYLHYLNVDDFTITRYNMNINGDDHVSFSAMEFSVTDKYLLVSTDRNRLILFKLHSDKQMRNFYGASNDQYTTTRNKIDPSGKYVYSTSQDHYLFVWEIATQKVVAKLDHHKLSVRDLDFSPNRDLLASCGFDKKVCLWSISKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT12</accession>
    <name>A0A8J4PT12_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_005707</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000235">
        <property type="protein sequence ID" value="KAF2072967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT12"/>
    <dbReference type="OrthoDB" id="15688at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008111">
        <property type="entry name" value="RNA-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45894">
        <property type="entry name" value="RNA-BINDING PROTEIN 8A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="73"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="167"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2072967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="197" mass="21792" checksum="CD3D0155DFF1977C" modified="2022-05-25" version="1">MSEEREKIFTEDLRKGIFPSKTKGRGINATESMEVEMEERYSGKGGVFDSLPVATGTDGDDQDGQPLKSVEGYIIFVANIQEEVQEPDIRDVFSEWGQIRNVHLNIDRRTGFTKGYALIEYQSKEEAEDAVKNGNGFELAGQKLDVGFAFISSSSSSGPVISVNRSISSASSSYRNKNGGYRGGDRDSKKKYSGKRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT45</accession>
    <name>A0A8J4PT45_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009740</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000793">
        <property type="protein sequence ID" value="KAF2068936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT45"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035256664" evidence="2">
        <location>
            <begin position="22"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="281" mass="34204" checksum="2B97A9F0852A0A1D" modified="2022-05-25" version="1" fragment="single">MKFILLTLLLIAIWSAGQTYAFNQFDNDLPDVFPAGQWEGNNNNNNKWHQWKSDSESDFDPRPRPRRTDSDSDREGRRNKWHDAHPEVKRRSDSDRDDWRSDSDSDREGRRNKWHDPKPQPIRSDSDREDRRNKWHNAHPDVKRSDSDNEWEPRPIRSGSDSDREGRRNKWHKDHQNFQKRSDSDRDDWRSGSDSDREDRRNKWHNDHPEVPRPQPIRSDSERDNWRSDSDSDREDRRNKWHKAHPNFPRRSDSDRDDWRSDSDSDFDWRSDSERDNWRSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT71</accession>
    <name>A0A8J4PT71_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_005640</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000230">
        <property type="protein sequence ID" value="KAF2073057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT71"/>
    <dbReference type="OrthoDB" id="18015at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000388">
        <property type="entry name" value="Putative RING zinc finger domain superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051834">
        <property type="entry name" value="RING_finger_E3_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931:SF17">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="273"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="242"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="12"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="258"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2073057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="318" mass="35457" checksum="E2646C1321FAF218" modified="2022-05-25" version="1">MSSLVHNRETSTESSTGTNSSNSSTYTSSSSNSSGRTRVTTPRNRNNNSNSSMGLKNFIAKNINLLILVTIISLSIYTLLSLITPSSLIWVFALIAVAGTFLQIWYGFRYTTRIIEVNGQYHIYPANASNLLFNNNNNNSSSSSTNPTSQNRMFLSNSRFSHLINPPLNLQLTFIDRDFDSNDYDMLLALDDDNQNYGGAKKEQIDLLPLHSLSSKKDLESLFNSGCMDFCKTEIKETKKDEADTSSSGSKVDNIDDSSSSSNLNNSQQQKDCSICLEGFKIGDSIRTLPCVHHYHRECIDQWLKIKSVCPVCKLDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTK7</accession>
    <name>A0A8J4PTK7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_006382</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase gamma subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000292">
        <property type="protein sequence ID" value="KAF2072304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTK7"/>
    <dbReference type="OrthoDB" id="449052at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04618">
        <property type="entry name" value="CBS_euAMPK_gamma-like_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04641">
        <property type="entry name" value="CBS_euAMPK_gamma-like_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF35">
        <property type="entry name" value="LD22662P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0129">CBS domain</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="6">
        <location>
            <begin position="353"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="6">
        <location>
            <begin position="427"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="6">
        <location>
            <begin position="506"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="37"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="127"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="165"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="187"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="223"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2072304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="562" mass="63051" checksum="B963354514D22351" modified="2022-05-25" version="1">MWYEDDDELSLELAKLDKQEIEYLKIKQEQHIEQLNKKHQEEALQQEQEDKERLDNNSKSKNTVNNNNNNKMNNNSDRIISSSIDDEIRIPSSAMKRSSSPSPSPSPSPSIISSSQDAVFNIDDIQQPQPDTADRLPRFTNNLPQSTNNDNNNSSSSSNPLKKVSTLTPSTDSLQPPTPSSPRETDTTTTTTTTSSTSSHNNNNNNNNNNIPTIITSTPATNRGTRSLSSGSSVNMTMPKITTEIEKNIEEGKMLFCNFLKGHACYDVIPISGKVVVLDTRLVVKSAFYALEENGIKSAPLWSSELQDFTGMITVSDFIDILLYYYHKPKSDNIFQDMGIHRIETFWREIDVERPSSLISTEPETNLYEAASILLHFKIHRLPVVDKKDTNSILHILTHSRILAFMMKSLPQLPEKLLSIPIGSLGIGTFQNVVVVMTQTPLAEVLTLLSEKKISAVPIIDSETSKIVDVYSKSDVTLMAKQGVLSPTDLDLPVHQVLSTFTKLWQRPEQVYTCTRFDKLGDVIDKCLRKRVHRLVCIDSSKKVEGIISLSDILNYLLNVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTQ9</accession>
    <name>A0A8J4PTQ9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DWNN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_004165</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000142">
        <property type="protein sequence ID" value="KAF2074538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTQ9"/>
    <dbReference type="OrthoDB" id="106784at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
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        <property type="term" value="F:ubiquitin protein ligase activity"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014891">
        <property type="entry name" value="DWNN_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033489">
        <property type="entry name" value="RBBP6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025829">
        <property type="entry name" value="Zn_knuckle_CX2CX3GHX4C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15439:SF0">
        <property type="entry name" value="CELL DIVISION CYCLE AND APOPTOSIS REGULATOR PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15439">
        <property type="entry name" value="RETINOBLASTOMA-BINDING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08783">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zf-CCHC_2"/>
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    </dbReference>
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        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DWNN" evidence="9">
        <location>
            <begin position="3"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="8">
        <location>
            <begin position="194"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="157"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="364"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="236"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="364"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="428"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="446"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="473"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="495"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="513"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="524"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2074538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="586" mass="66235" checksum="DCDED5163A271700" modified="2022-05-25" version="1">MSVHFKFKSAKDAYDSVTFDGGFISVADLKKIITTKAKLGKSTDIDLVISNSQTGEDYKDETFLIPKNTSVIVQRVPVTSSRFNMNQNVNQQPSAPIPAPAPAPLIIPQNEFGNDPLGVDQENLKINALINQSGTSATIGGVPTGSHMGGGGRFYQNNNDGGRHHHHHHQPYNPQGNPASMVKSDKPPPASYVCHRCFQTGHYINQCPTNGDANFDKRKIKKPSGIPATFLKPIQPSSTPTASTPGGPPTVLMPGGGVAQVMANESEFTRMAKAILVMEEENKKKQEEAATTPVDPSTLFSCDLCKQSNDIQYQIQLQCCNKNCCLNCLKTNLDSKDKVCPSCSNPLVIAMESQNRIQTLGFNLNNSNNNNNNNNFENSDYNNNNNNSYNNNNNYNNNNNSYQQNNFDNNNNSYDANNYSNNRGNHNYNKDFNRNERDSNSNYRNDYNRNDSRHEYRDYNRNDSRDNRDSRSNDYNSNNGGSSRYHSSSSSSHYHQSDDSRDYRSSNSRDYRSSSSSSGSSGDRNYRDSYRSNDRNDRDRDRDREKSGPPRERERDRDRERDISREKDSRDYRNDRDRRDSSSRKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTS3</accession>
    <name>A0A8J4PTS3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histidine kinase</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">CYY_006284</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000283">
        <property type="protein sequence ID" value="KAF2072409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTS3"/>
    <dbReference type="OrthoDB" id="18372at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16922">
        <property type="entry name" value="HATPase_EvgS-ArcB-TorS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000004">
        <property type="entry name" value="Ethylene receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000010">
        <property type="entry name" value="Sensor histidine kinase RcsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339:SF1">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 14" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0807">Transducer</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="490"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="517"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="542"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="593"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="629"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="17">
        <location>
            <begin position="684"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="18">
        <location>
            <begin position="937"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="152"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="273"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="359"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="410"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="280"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="14">
        <location>
            <position position="986"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF2072409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1071" mass="121891" checksum="FFE0148CA0E684B7" modified="2022-05-25" version="1">MLTHTYHRKDNFSYHQQEELIGLRSCGINQKPILHRHQFQHQQQPQPQPSSFSTTGYASKMITLDNTRSGVNLSLQSMLPTKPNQILSLYNSKEPFDFHYKKIPSSIDQFQYQQQQTQQQQIQFDQNQNQKQIEEKRQFEIEKQISQPSSPLLLPNIINNNSNDNSNYSNSNNSRSVDQEINNIQQQQQQSFQQDNYNNNSNRYFRFIKKFEKVNIIRKFKTNSRMLFSDIMEYWYPQKDEILFETDQNYLFNDIQSTDSSEFYDDTDFESDIDEANFNKPTISISSPRKPSPSSSSTTTTLLSKTTTTTTTTTTNTTSTLIDNTPITNKKHIDEKVFINEQPTQPQPQPQQQNIQYLQPQPQPQPQSLPKHPEKTNFKKRSAQTPSPCFKSDTLSTTSPTTTAFAKVSTSIDQNNNNNNNNSHSNLGVEEDDKLSTYSNNTINNLTNASSNINNHKNVIYKLVAIFNMICGIVLKDFNFKSQEKYKAKLVIGFCFTIISFIPSWIIFFWLSDINKPAIMAIVALPMAISSLFMLKKTRSIYYPCHILCFTLCFALTVNSYYTGGHQSTIRLLMTEIPIIAALILGRKASLQWSGIVLSIYLIFFAANLKGFEYIDGIPNITIRSHMNFIIDVTIVLITLAFTLCYQYFIDEAHRETKLKNAQLTIAKDAAIEAYQARQEFLATMSHEIRTPLNGLIGMATLLRDSNNLPPEEKQMAKSVKSCGDILLRLVNDILDLSKLEANQMGLEIIPFKIRDLAQQICQVLSGQANERNIYLNFDISEKVPLVLTGDTGRILQILMNLTGNALKFTQSGSVTIIIDLIEDDNEKVSFQEGVYNICFRVKDTGIGVPVEAQQKIFEAFVQADPSDSRKYGGSGLGLYLCAKLVKLMKGEIGVYNNPECIGSTFWFIVPLGHGSESAIDSIAQSADIPYSPNVIKVLIAEDNIINQRVAVKFLEKVGIKAEVASNGNEVLQILEKDHFDLIFMDFQMPILDGLRCSKAIREFERNGKWSQYKSSIFICGLTANTMSTDKKRCFDHGMNHFISKPFQIQQLRGAIDMAIKHKEMSNMLHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTZ8</accession>
    <name>A0A8J4PTZ8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7831 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000197">
        <property type="protein sequence ID" value="KAF2073552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTZ8"/>
    <dbReference type="OrthoDB" id="18511at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057153">
        <property type="entry name" value="DUF7831"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25176">
        <property type="entry name" value="DUF7831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7831" evidence="1">
        <location>
            <begin position="11"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="134" mass="15236" checksum="F243EA5B16852BDD" modified="2022-05-25" version="1">MTSEKISKLVIYKGNWTAKIVQSTPKSLYIFGDNDKKIGRKGQACIRFCKNAIGIPTKKGPYLYESSFYTDDEYIDNVLKIDLHIDKIVKISVNYNHIVLPEAGLGTGLAQLNTRAPRTFEYLEKALKERLHVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU42</accession>
    <name>A0A8J4PU42_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_006078</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000264">
        <property type="protein sequence ID" value="KAF2072619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU42"/>
    <dbReference type="OrthoDB" id="10256233at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47960">
        <property type="entry name" value="DEAD-BOX ATP-DEPENDENT RNA HELICASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="9">
        <location>
            <begin position="91"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="127"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="457"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="527"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="5">
        <location>
            <begin position="91"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2072619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="638" mass="73352" checksum="A8C823535EB887BF" modified="2022-05-25" version="1">MNQIFKFTTSKLLNKPFTLNNTTQLILSNYLYNSPSQHHNSNQCIKDKQQNNINRFYTTTTTTFNNNNSSSDNSDTTLKNDIFSINKQQQQQQQSFNYSKQVIQALKELNITEPTPIQKKIIKAFDKSKSTTPNDIFILDETGTGKSYAIVCEIVDRYYQYLKSNTNQSTTTTNSATQEETTGAKTNVQPKYLLISPSRELSWQLAKWIRDLLIKLPGVKESDLSKIQLAVGGIPTEVLKQTLEIESPDIIIGTPKVLYELMITGNLGIEQLDLLFIDEADHIFQPLKLHAIEKEKKIRKAHPLPSHLLFNEIKRQCRENNQIERDDDPIDAQQLLKMKLANKHQQQQQQQPPIKKYKKTARQFQSVFTSATLTSVTRRLIISGDWINKNFDLISIDKSNISNSSSNSSQNSNNVLDRLEYYFISVDSEKDILNGVVDVWKHLKPKLAFGIIANDANITNVVEQLSKRNINTKPLVSMMDFENFNICHWTDSNTKDSIKSLINQDALDKNRLLKKDLKEKQKKREIETRLKNLSQPTKKQDDDEYDVQENQRFYQKDKESILYIAKEDAIRGIDIAQVSHVFILNVPVNTNNFLHMIGRTARMGNKGNIVSIYHPALSSKYNSTMKSLNIPFEEESLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUK2</accession>
    <name>A0A8J4PUK2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NADH dehydrogenase [ubiquinone] iron-sulfur protein 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Complex I-15 kDa</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">NADH-ubiquinone oxidoreductase 15 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_008829</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Mammalian complex I is composed of 45 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the complex I NDUFS5 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000585">
        <property type="protein sequence ID" value="KAF2069851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUK2"/>
    <dbReference type="OrthoDB" id="9992197at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24141">
        <property type="entry name" value="NDUFS5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019342">
        <property type="entry name" value="NADH_UbQ_OxRdtase_FeS-su5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15224">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15224:SF1">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="14"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR619342-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2069851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="87" mass="9984" checksum="89882B10BDDCB2C2" modified="2022-05-25" version="1">MASGFGPWGGVGKCYPMWSDFCTCIYEKPLELCTPYRADYMECISNTKEYKSQLSENYHDGSEARLAKVREAIRRVNEYEPKVGKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUP5</accession>
    <name>A0A8J4PUP5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FYVE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_008755</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000572">
        <property type="protein sequence ID" value="KAF2069926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUP5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000285">
        <property type="term" value="F:1-phosphatidylinositol-3-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013899">
        <property type="entry name" value="DUF1771"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45748">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45748:SF7">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08590">
        <property type="entry name" value="DUF1771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01162">
        <property type="entry name" value="DUF1771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="7">
        <location>
            <begin position="47"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="127"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="182"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="127"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2069926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="387" mass="43857" checksum="DB339F33CD0B1283" modified="2022-05-25" version="1">MQTAIGDEDDEQDISIIEDDEIKSTNNTSSGGGGRNELRFSSEWVPDNQVTGCKACKVQFTITIRRHHCRCCGCIFCSKCSRNKIPLQSNELNFKYIKARVCDKCFSLSLKTSYFEKVDIKVSSPLRQSTSSSSLSNSASSSTISLPSSMSNSQLCDNTSNSSYSSNNNNNNNNSKWNKGTIVTEKDINHSVQEEIKEIQQEYKKLREQANELQIQRDESYRKATEYLQKGHNDLYQSMLNKGKSCHQQMEETNWKAARKIFYERNYHTLTSIDQSNTIHSIDFYGLSLNETIVIFQEKIKLFQLNLINNSVLNLINIGQDNNTSSSNNVNTNTTTTTAAAVAEDNIVEDLKNYLQLYFKNDVKDLKGSVVVVILGDRHIQLLFSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUW4</accession>
    <name>A0A8J4PUW4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cytochrome P450 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_004681</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000170">
        <property type="protein sequence ID" value="KAF2074005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUW4"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20617">
        <property type="entry name" value="CYP1_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000068">
        <property type="entry name" value="Probable cytochrome P450 508A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF375">
        <property type="entry name" value="CYTOCHROME P450 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12 13" id="KW-0349">Heme</keyword>
    <keyword evidence="9 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035275734" description="Cytochrome P450 family protein" evidence="14">
        <location>
            <begin position="21"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="429"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2074005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="484" mass="54693" checksum="92A76A4B362A948C" modified="2022-05-25" version="1">MIITIAFIVLIFLLTRFLKSQQQSREYVNLPPGPTPLPLLGNLLSLVKDSHISISKLAKKYGGAMTLYLGNVRTIVISDPNLIKQVFVNQSQDSTNRFILETAKIIGNGKNILFSNGDYWKQYRLVLSRSFMRLKTHSIMTDKINKEAEYFSNSFKSNPIIDPAHFCKIFTSNVMMEMLFNDRSKYSNTETHKIIEAIKNVEHGLAVGSSFDALPFLKLFFPNARKDLIKTLEDVWEYSKESIVNHQNSLAKDENQINDFLDLLLTEIKGSGNESFYDMEGLYRVCSDLLISGTETSASTMTWLILYMINNKDIQMKARKELQDAMGDKKYLTMEDKSKTPFFNACIKEVLRIAPVGALSLPREATADLEVGGYLIPKDSQLIMNVYGLATNAELWSDPLIFNPNRWLSEDQEMSNYQYIPFGIGSRACIGSSLAKDEIFLGVGNLLLNYEIDSENGKPLTEKGNFGIALCSQPYQAKFTPLVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUZ0</accession>
    <name>A0A8J4PUZ0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_003317</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus outer membrane</location>
            <topology evidence="6">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TMEM53 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000102">
        <property type="protein sequence ID" value="KAF2075393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUZ0"/>
    <dbReference type="OrthoDB" id="77878at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005640">
        <property type="term" value="C:nuclear outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008547">
        <property type="entry name" value="DUF829_TMEM53"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12265">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12265:SF30">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05705">
        <property type="entry name" value="DUF829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="257" mass="29855" checksum="B71876B888E76023" modified="2022-05-25" version="1">MKYPNLEKTKCFYSTPNDQNEIDHSKSLVILYGWMGAPRPILAKYINTWLSKGCNAFSAQTPFDPTLIVVKKKGIRLGYQIGEYIKRHPECKTILLHQFSNGGTCTFSFSIEIIHKFYPQVIPLIKGIFLDCGPSSTFKSMKHAFHSAIVNHGPKRIRPLILLKYVFSKRWNQTDRLFWLSEMKCNFIVVSNKEDDIIDYREVCQFAERIRTTKANNQVIDNSIVIEKVFEKGGHVKNLQFYKDEYESLLDKLIEIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVD6</accession>
    <name>A0A8J4PVD6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000203">
        <property type="protein sequence ID" value="KAF2073444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVD6"/>
    <dbReference type="OrthoDB" id="1415503at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="29257" checksum="54480CA61D35E989" modified="2022-05-25" version="1">MIIDYPNQLDPGVLPLGLEKLYLGKYNHPLSNPTNILPSTITKLILPNYNQPISPGQMPSSLVFLEMREFNKPLVEGALPFGTLEKLSLPIYNHPFPSTACQSDQITLNHIKVFKLDQTITNVISSRITNLQLEFAQKQYPVKLPNSITNLKLEAFGDKAFTGTIKDLLPMHVKFITLTQLTLSPNLIPISCHTLVTDQLDFNRGDIPSNIQYLKIQNFAYRFKEFIEKYSKPDSFDIIPIKNVSLRSELTTLSIKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVE9</accession>
    <name>A0A8J4PVE9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004303</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000149">
        <property type="protein sequence ID" value="KAF2074407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVE9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052050">
        <property type="entry name" value="SecEffector_AnkRepeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586:SF3">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="747" mass="87243" checksum="F4744C20C962E200" modified="2022-05-25" version="1">MNRNSNSNNTRNRDHRNNQNNRNQNNRNNNNRNNRNIYRKRDSKSDGTLALHEQLFKRIWGSVILRKEILRHLVSNDRGLDHLRHWEAHKVPRDGLLNGNVLQLPKRYHDVSMSDTTWIANNHHFGLLVDRLMHPMYQPLTVDYQAIIQFLRCNTEYKLFIRLYNFNAELFQRVNGFLIPRSSRDEKSKRGGRGSRGRRGGHVGRDESKTLPTTTTKEKSKSIGDLACQSGSTQIVEFLVAKRYKITIEGVYNAVKSNSVDLVKLLLSFNHLRIPHHQPLMNDAVSNGNMDMIKLLVENGYVQCDSDHLKLLFKKKNCIQVLEYLVDHCKHLFVRNNQPLYSIAGRGHLQLFKRLYEIIDKSTIDFKLEGFIVSSGNLELSKYYYSSVFVGYSHITSKTMDMVVSRGDFKTYTNLKNKANPSKQGFLNALSNKQWQAVSFLSKIHLNSFIFDLNEKRFIFGSLLMNGQLELLKYFYKEIYLPERLDYCVKVYHILKAVENKHYDALTFYFKFFNYPPHFLYMLCQLEGKYEVIQLIFKTLRASKREVKLCSHRLIQGAMIGGNHNYIALIKRNPACTNTYCGYNYVQIAKTRSLMYAVQMSIGEIHLVPYGCRYSDSVYNFKLALQNKSIHMDINEFIRHFVSNNDVGMLYYIKRNCPNLLSKIDSFLCHRFTHGKTKITANPSTMALLLKDQNSLLYLHHLRINNLLVADDFAFKEIQDLVKRGVPRPYVTSWPVVRFIKKYKLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVF6</accession>
    <name>A0A8J4PVF6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Geranylgeranyl transferase type-2 subunit alpha</fullName>
            <ecNumber evidence="2 9">2.5.1.60</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 9">Geranylgeranyl transferase type II subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_005222</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to cysteines occuring in specific C-terminal amino acid sequences.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>geranylgeranyl diphosphate + L-cysteinyl-[protein] = S-geranylgeranyl-L-cysteinyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21240"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11537"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:86021"/>
            <dbReference type="EC" id="2.5.1.60"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the protein prenyltransferase subunit alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.60" evidence="2 9"/>
    <dbReference type="EMBL" id="AJWJ01000202">
        <property type="protein sequence ID" value="KAF2073474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVF6"/>
    <dbReference type="OrthoDB" id="1658at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005968">
        <property type="term" value="C:Rab-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004663">
        <property type="term" value="F:Rab geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097354">
        <property type="term" value="P:prenylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.120:FF:000035">
        <property type="entry name" value="Geranylgeranyl transferase type-2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.120">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002088">
        <property type="entry name" value="Prenyl_trans_a"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11129:SF2">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE-2 SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11129">
        <property type="entry name" value="PROTEIN FARNESYLTRANSFERASE ALPHA SUBUNIT/RAB GERANYLGERANYL TRANSFERASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01239">
        <property type="entry name" value="PPTA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48439">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51147">
        <property type="entry name" value="PFTA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2073474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="311" mass="37752" checksum="758870B158E1639A" modified="2022-05-25" version="1">MHGVLKVRTTEEKAKAARILELKKIEEYNSIVKKYTDNRESEQGKYNETSLSLSQKVLEENPEFYSVWNYRKSIFNQFKETKENEELQKLYLNEMKFIEECIQRFPKSYWIWYHRKWISLKLDSCDWERETKLCSKLLNLDLRNFHCWSYRRFIIQHSNIPLEKELEYTTTKIEQNFSNYSSWHQRSSILPKIYTDAEEFIAKLQEEFEFVRNATFTEPKDSSSWIYHKWLVHTIKQIPNSNYIDILKNELEQTNELIELEPDCKWPILISLLLKIEIGAYPQDELKDQINLLIKLDNDHINYYKSLFNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVH3</accession>
    <name>A0A8J4PVH3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Acyl-coenzyme A oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002966</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000088">
        <property type="protein sequence ID" value="KAF2075723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVH3"/>
    <dbReference type="OrthoDB" id="538336at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003997">
        <property type="term" value="F:acyl-CoA oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033540">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA oxidase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012258">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909:SF382">
        <property type="entry name" value="ACYL-COENZYME A OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909">
        <property type="entry name" value="ELECTRON TRANSPORT OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2075723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="587" mass="66214" checksum="459A56354BFE7615" modified="2022-05-25" version="1">MSPTHAEIKVKLRELAKSERFQKDVQTLSKEEQVENLYNLMTEVGEIAQVKADDIINDLGKVFAVMESPCLIFQYGLGTLLGTHYNLGMGTIMQLIGDNREQLQDYIDDLENFRYWGIFMATELDCGSNAMSMQTEAHFDVNTQEFIINTPSLGACKFMPYLSSKHQPKMAIVMARLWLSGQDCGIHPFIVHCRDRNGQLLPGVYASKAATIDMYRVCEVDHSITAFKNVRIPHKGFLGGALNRVTRDGKFVTKAKNKRDIFFQSFCRVESGKMVLIAALMPMFKLAISVAIEYSKKRRIMTKGGTESLTKVLSHKLDLAKAYISLTGSICLYEKMKRICLSEKLPQEKHQYCNETVKTMCVEVAREALQICLARTGAQGKLIRNRIVTAILANDKASTAEGDNLPVMLKIAKDLLFNDYQPLSCNDNDITLPITHKQRLLFLLHTQEKIAKRVLREKLMESGNPADAWIHSTQLARHLAWAHGMCEMADSLKHRSDIQDTFCMLHILKEGSWFIINSLVTKDELETLASVSEKNLSTIFDQQAVHVAKEYDIVDILALTPIGNKNTAKAWCDISDFNFTPIPSSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVL1</accession>
    <name>A0A8J4PVL1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002881</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000085">
        <property type="protein sequence ID" value="KAF2075798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVL1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2075798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="161" mass="18438" checksum="DEE9B9D66E59ECC5" modified="2022-05-25" version="1">MEEIIKVNEECRKAYKAGSPTSYLFSLDKINQDYFIDDEYKKVLDTLKASINLQQEIKNVQQQQLIIKPIPCHSGFQQINKKDIEFLTITTQSSITSNTNTTTKIETDYNLNNDEPPFYTPSTSPPPMICPKAPTKPIPKNQFKHQKLMAMNRGSLDFSNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVN7</accession>
    <name>A0A8J4PVN7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_005083</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="AJWJ01000194">
        <property type="protein sequence ID" value="KAF2073594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVN7"/>
    <dbReference type="OrthoDB" id="17792at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd14003">
        <property type="entry name" value="STKc_AMPK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000060">
        <property type="entry name" value="Sensor protein FixL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 10 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 10 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="16">
        <location>
            <begin position="10"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="16">
        <location>
            <begin position="122"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="499"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="283"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="360"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="783"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="885"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="983"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1016"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="289"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="363"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="416"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="437"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="451"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="793"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="885"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="939"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="985"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="641"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 12">
        <location>
            <position position="528"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="659"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="11">
        <location>
            <position position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2073594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1084" mass="121340" checksum="3C43D05F99BF1361" modified="2022-05-25" version="1">MNNPAHFQQRSMDSAIVTINDKGFIQSVDRKTCELFGYTIEELQLQKVNILIPSPYKEQHDTYMQNYFETGVKKIIDKSRVVEGLHKDGSVFPITLSVTEVKIWNKRMFIGSIEPVIDKRLIIYTDVNGIITYCNRNVEDLIGYTPPEMFGKNVSSLMPSPHAKNHSDYIEKNYHGGGLWKMLNRVRNLPIKHKNGVVFLVSILVTKISTDGIDMFKAIIQTPPKEAVFTVDGEGIIKASNFNFTEPMFGYTQKDLVGNTIGMLIPEMSKLIDSSVVNNSSSINNTTDGLSSPSSTGSHADGEQPVGNRGIESWLGRTRKVDVWHKDGSRFPVNLEILKLQGEGLNFSIQIKKVVDNPRGLYNKKENSSSKEGKEGKEGKEGKEGKEKDKDNSSSSNSKKDSKDKKKRKKDKDKESESEESDSEENGDSNNNNNNSSKKKKSKSKKRKENNDDDEQSSESEDESDGGGGSGSGSNSSKKRKSRQQYSEYHAPPEVMGDYTLGRTLGRGNYGVVKLGTHITTKEEIAIKILYKEQMTESEFTRCKREIEILKQLYHPYINKLINVLENEDAMFILMEFCPGGDLLHYVNRHGLKGVKMVNSPDIGDQVLMGIPLSEDDTKRIFTQICLGIAHCHKLNIAHRDIKHKNILFDNQMNVKIIDFGLSNWSYQEKMSFCGTPAYAAPEMLLGINYNGPEVDIWSLGVILYSLVTGKLPFINVTDMIVGKFTLPQSIPLDLQDLIKKMLTVEREKRLNINHVINHPWLLKDPASVNLISTHSNLSNISLNSSADSPIVSSNNTTTTTTTTSTPTSNTTTATGSISSPLSSPMPMSINTPPTTTTTTTTSTSNSNNMVHTPNSQFIPPSPFEFPNNTEQKLYTTTDNNQTYFNNNNNSNNNNNNNNGNFYTHANTNSNNNESNNTNTNNNNGSFYNITSPTASVATPTNNSNNNISTPTQNATPPASVTSLSSPVEHQIEFNQQMAYYHHFPNSTTTPPTQIPPSLTGSITSTPFDLMTNELYQQQQQQIQQLQQQVQQHQLQAQQLQQQAAQAAQALQQQQQQQQQQQQQQQQQQQQQQQQQQQQQNNFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWR6</accession>
    <name>A0A8J4PWR6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003616</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000115">
        <property type="protein sequence ID" value="KAF2075096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWR6"/>
    <dbReference type="OrthoDB" id="15815at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052867">
        <property type="entry name" value="ATP_Synthase_Subunit_6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34565">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34565:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="68" mass="7827" checksum="EB838C7FE12F6F47" modified="2022-05-25" version="1">MRQEPLASYLRTQGKIYWPFLIGVGVWGALISKMYAGITEEDKKNSYYWKKIEAKLHPQSHGGSHGHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXB1</accession>
    <name>A0A8J4PXB1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Myb domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_004130</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000141">
        <property type="protein sequence ID" value="KAF2074547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXB1"/>
    <dbReference type="OrthoDB" id="118550at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44042:SF15">
        <property type="entry name" value="DUPLICATED HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44042">
        <property type="entry name" value="DUPLICATED HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SANT" evidence="6">
        <location>
            <begin position="286"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="288"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="291"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="19"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="144"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="361"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="441"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="216"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="265"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="370"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="403"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="446"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="464"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2074547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="633" mass="71894" checksum="0222DDF52FC90CB4" modified="2022-05-25" version="1">MFSPNTDNSENIMYISNGDEMYGNTYQDNSNSTSNSNQHQSKNESNGSNSSKKKPLGFFSSNSFHTNGNSGIFNSAFFGSLFPSENIDQIPNMLNSSEFLEKNFGSVHNFHTFFNSFSSSGQLLNPQQQQTLLAIQNTIQQQQQQQQQQQQQQQNTPSSSSSSNNDTPTNTESYQGLISSDSFERINLSNPSTPPDSPNRLDKNGEPISPDLESPNNHNNHNNNNNIIEKVSPPIQDKNKTTKSTTTTTTIMPVIAPTAAKGKKKKDEKNEELAKTADGKPVKRAPFKQGWTKEEHIRFLNGINIHGKGAWKEIAQIVATRTPTQIQSHAQKYYLRQKQETKNKRSIHDLSLQDLMAHENNISTKKIVQKSTSPTSSKSKSKSSSSSSKSSSLCSSSKQTTPPLQPTNQPPSPEYSYQSTDPVGKLFSPFYRTNTSVPEMKMGYYHNNNNNNNNSEGLNNNNNNHHHHHHHQQHHHDKLDIHSPPPQQQNGHQNSYMRSIPIQQFQRGPPQQSPIQSSVHLPSHIDQPNNYHNMYSPMNHNNHQQQNNIQQSYQQQPQYMVQQNQHWNSPPMIHNNNNNNNNIQDNMIQYNNSNMQPQLQHHHQNHHQNMYGYNPMINSYSPQIVNSSHLQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXM2</accession>
    <name>A0A8J4PXM2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001895</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000048">
        <property type="protein sequence ID" value="KAF2076818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXM2"/>
    <dbReference type="OrthoDB" id="20551at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048024">
        <property type="term" value="P:regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.980.20">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052225">
        <property type="entry name" value="Ser/Arg_repetitive_matrix"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23148">
        <property type="entry name" value="SERINE/ARGININE REGULATED NUCLEAR MATRIX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23148:SF0">
        <property type="entry name" value="SERINE_ARGININE REPETITIVE MATRIX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="481"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="724"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="804"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="889"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1079"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1135"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="184"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="274"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="309"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="493"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="522"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="543"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="724"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="764"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="821"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="889"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1084"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1135"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1154"/>
            <end position="1168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1168" mass="129368" checksum="66F8217769084E41" modified="2022-05-25" version="1">MSSSNNNNNNNSSSTSNSNSNVGNNNGNSNTNNNGNLVGMDKILLLCSTCHLKTNQDIKELKEKVLPGFHDNKYSFTCKSCTPNKVPTLNHLGKSWKCVIHTALFNMERQSGNRFFHHSDVTNYVIKYPNVFLHDKSLADISKAVTMAFFNNKHYFVSGGSYTGKWANPRPADVSDLDFDSPPNDSISNNNHHNQNSSNNNNNNNINNNNNNNQQQNFNSNDFDKERQLLSPSRSRSMSRSRSRSQSMSSRESRSRSRSRSVSRSLSRGRSRSRSISSRSNSRSISPRNNSLKRGVGGRSGSGSGGNFSTGSSRSSSLSDSDSDSNSDSESELFMKYIQCLTCKNLSTMDFSLNKVAPGPFDKNYEFICKGCNEENPSFKHLAKSWTKIIHTALHNLEFEKRIRFHSKKDIFQYIQSHPKELECDQKQDVTPNIINYVLMSGRSLFINSAINQRLWCNRKKKDRVLVDSRGIYVFAKELSKKRHHSEMDNERSSNNNSISNVVNNMNNSNNNNNINNNSKSSNDEKVHSHNESSHRSSILSYNGGNSKSSSSSTSQNNSTNSSNNNNNGNNLTSSIQQNINSSGNVAIQNIKFTPLFCNHCKVLNPNAASELLEETPGIIIPSHIDKRYIYVCAKCSDNQQATLNHLPKSWKVIVHTTLYNLGIISGQKFSSNKTLYSFISDNANTLLLGGKTIDYLNKNLSGVLSSYKNLFCSSGRNMWGNVSQYQENSNTGDNKNKATIDDDDEKNSEDEHSYHKIKKHKSSNSNSNSNNNNNSINSITDKLLNDDNNLMEIKSSKLMDTSVGKKDLNSNSPITFVKRPSSSSPSSYPSNSSPNSSILNSSGGNSGNQSRPSSHQPQKPNITTSPTNTSKNSSINNIQKELYNNVNNVNSNVNSNNKTTNNTTSTNNNNNNNRNLNLEDIQDETNVEIVIYDERIHSLIGFLHVNINTELNQLRKEVDDMEIPELKNLEFRFLFKKSPISLKQESHFNIKDCLFSGSNGITVELRSITPNVPVSPGISNGIGTLSNSGFNPNIAAAAAASLLQSPQPFNVSSVPDISAMSNHPMFILFSQFQQLQNQIGGPSSSSSTSSSSSSLSTMGKKDNILLNNNSKEDQILKAFDMEPHSSMLSSITSKLTPISTSPSANKKELDQIESANTKTNSILGLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXN7</accession>
    <name>A0A8J4PXN7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DUF307 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_001876</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000047">
        <property type="protein sequence ID" value="KAF2076848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXN7"/>
    <dbReference type="OrthoDB" id="16982at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015369">
        <property type="term" value="F:calcium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000014">
        <property type="entry name" value="Cation/H+ exchanger protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004713">
        <property type="entry name" value="CaH_exchang"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005185">
        <property type="entry name" value="YccF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503">
        <property type="entry name" value="VACUOLAR CALCIUM ION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503:SF10">
        <property type="entry name" value="VNX1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03733">
        <property type="entry name" value="YccF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="145"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="237"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="375"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="408"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="441"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="509"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="630"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="666"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="696"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="domain" description="Inner membrane component" evidence="11">
        <location>
            <begin position="142"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="10">
        <location>
            <begin position="344"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="10">
        <location>
            <begin position="567"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="27"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2076848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="719" mass="79534" checksum="231EFAA4E8FA7AFD" modified="2022-05-25" version="1">MSDIPLLAKGEGNDGYNSIGDTSVSFNNNSNNNNNLNSSTGINNNGTTTGINNNSSINNTPVKTKHRKSIYAVEPDIPLTYEDLGLSPSADPIQAKRAIQNHMFGFKPFKSSLTKKKFEDRSPEVKHLYNDNFSIEKSVRSIGNYIYAATFGVILFIIFTILSLLMFITYFGIPYGKLLWQIKGFVLWPFGKYLQLSKSSNIMDDGWGDESQPLSRKYRNDGSLGHKQSVSTIIGGVFYYLLCAPVIVIFEVLVLSITWMVVVMIPTAKLHTKLLSNLLTNPLLLRVSKNPRLDCQILLYPLESFNRYYYKYTINGMNVCLVNLLPFVILSLIFGFFLQNYIHPVVIFLCCMLSTIPLSYYNGMAIASISAQTSFAIGALINASFGSIIELILYVVSINGSQPEVARSALTGALLGAMLLIPGLSMIVGGIKHKEQRFNPAIMGVSNVLLLIAVIGAFFPTLFYKIYGNYSLNCSECVTVNGTMNCGSCAYVQGNLNDDPIYTQKARTLMYICAGILPLTYFIGLLFTLKTHTAILHNSDKHDDDQHDDQGHGHPKGEYEWSKIQCVAVLCVCTTMFALVSEKLVATIDPVVEAFDLTHEFLGVTVLGIIPSAAEYLNAVQFSLNNNMPLALEIGASAAVQIMLFQMPVLVFICAIINHMSVDGSYTLISPMMDFFSVFFGVLVMSLVFGNGRTNYFIGSILVIVYLIIVFCFYFTPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYF3</accession>
    <name>A0A8J4PYF3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">STI1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_002675</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000076">
        <property type="protein sequence ID" value="KAF2076015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYF3"/>
    <dbReference type="OrthoDB" id="2423701at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000020">
        <property type="entry name" value="Stress-induced phosphoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.100:FF:000002">
        <property type="entry name" value="Stress-induced-phosphoprotein 1 (Hsp70/Hsp90-organizing)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000027">
        <property type="entry name" value="stress-induced-phosphoprotein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041243">
        <property type="entry name" value="STI1/HOP_DP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006636">
        <property type="entry name" value="STI1_HS-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058209">
        <property type="entry name" value="TPR_BSK1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904:SF523">
        <property type="entry name" value="STRESS-INDUCED-PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904">
        <property type="entry name" value="TPR REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17830">
        <property type="entry name" value="STI1-HOP_DP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25575">
        <property type="entry name" value="TPR_BSK1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00727">
        <property type="entry name" value="STI1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="8"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="STI1" evidence="7">
        <location>
            <begin position="130"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="237"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="312"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="372"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="440"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="STI1" evidence="7">
        <location>
            <begin position="506"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="187"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="209"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2076015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="557" mass="62764" checksum="DB817A2D543152F6" modified="2022-05-25" version="1">MSDNSQKALEAKNKGNSAFSAKNYQEAIQHFTDAIQLDPTNHILFSNRSASYNALNQFDKALEDANSTITLKPDWSKGYLRQANALFGQSKLEEAEEACNKGLSYEPDNAQLKSLQQDITGANSMSNPFTPASINTLKTNPKTKDFFSQPDYLMKIADLSSNPKNLTKYMDDPRITKSLGVILGFDLDSFGQQPPQQPTPTPQQQYKPQPTPTPQPPKPTPQPVKKETPPPPPKTDAEKQRDLGNQAYKEKKFEEAIKHYDQALELDPNDILAMNNKVAVYLEQQNFAEAESSAKKALAKADEIRADYRIKSKVYTRLGNVYLKQDRVADAYKAYSSAILEDKNPETSANMKKIEKIKKEHDDKAYINPELSLVAKNQGIEHFKKGEFPEAIKSFEEAIRRNPQDHTIYSNRSATYAKLCEYPLASKDADKCIELDPKFIKGYIRKGNALFAMREYQKALEAFDQGLRIDENNQELLDLSRKTVFALNKSQNEMTDEERLEQASKDPEISAILQDPVMNQILKDMSSNPSAAQNHLKNPVIAEKFKKLVRAGVVKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYU2</accession>
    <name>A0A8J4PYU2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Acetyl-coenzyme A synthetase</fullName>
            <ecNumber evidence="5">6.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_004044</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>acetate + ATP + CoA = acetyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23176"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="5"/>
    <dbReference type="EMBL" id="AJWJ01000136">
        <property type="protein sequence ID" value="KAF2074649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYU2"/>
    <dbReference type="OrthoDB" id="1706066at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019427">
        <property type="term" value="P:acetyl-CoA biosynthetic process from acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05966">
        <property type="entry name" value="ACS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000001">
        <property type="entry name" value="Acetyl-coenzyme A synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011904">
        <property type="entry name" value="Ac_CoA_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02188">
        <property type="entry name" value="Ac_CoA_lig_AcsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001208">
        <property type="entry name" value="PRK00174.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF14">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="8">
        <location>
            <begin position="51"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="6">
        <location>
            <begin position="109"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="7">
        <location>
            <begin position="558"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2074649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="682" mass="76283" checksum="FF0F31DBB9139C90" modified="2022-05-25" version="1">MMKDDEMINRGPVNISTKKEASALLANSSDTVFTQTNQFSSHCLIPTIDNYKQLYQASVDTPDQFWDEQAKKHLDWFTPFTQVSTGNFIQGDIGWFLNGKLNVSYNCVDRHLKENKDKIAIIYEGDKPSQIEKITYYELYLQVCRLANLLLSLGIKKGDTVAIYLPNTPVAIYSMLACARIGAIHSVIFAGFGFESIISRVKDANCRLIITADEGVRGGKTIPLKKKIDEVVCHCKTVEHVLVFKNTGGHVDFNPQKDIWAEEAMLEQRPYCPPEHLDSEDPLFILYTSGSTGQPKGLVHTQAGYLLYAAMTHKYVFDYQNDDIYACVADVGWITGHSYIVYGPLANGATTFIFEGTPTYPTPSRYWEMIERHKITQFYTAPTAIRSLMKFPTSFVEKSNLDTLRVLGSVGEPINPEAWRWYNKFVGRERCSIVDTYWQTESGGHLITPLPGAIPTKPGSATLPFFGIIPKILDPQTGQVLDNADTTEGVLAISRPWPGIARTVYKSHSRYLLTYMKPYPGYYFTGDGVKRDKDGYYWIEGRVDDVINVAGHRLGTAELESALVGSYCCAEAAVVGYPHDVKGQGILAFCILKEGYEDDENTVSILRNEVRNTVGPFASPDVIVITPSLPKTRSGKIMRRILRKIACHESSPEQLGDISTLAEPEVVPLLITKVLKYIPKTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ53</accession>
    <name>A0A8J4PZ53_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">H/ACA ribonucleoprotein complex non-core subunit NAF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_000845</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000017">
        <property type="protein sequence ID" value="KAF2077883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ53"/>
    <dbReference type="OrthoDB" id="21550at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005732">
        <property type="term" value="C:sno(s)RNA-containing ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000493">
        <property type="term" value="P:box H/ACA snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.230">
        <property type="entry name" value="Probable tRNA pseudouridine synthase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038664">
        <property type="entry name" value="Gar1/Naf1_Cbf5-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007504">
        <property type="entry name" value="H/ACA_rnp_Gar1/Naf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040309">
        <property type="entry name" value="Naf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31633">
        <property type="entry name" value="H/ACA RIBONUCLEOPROTEIN COMPLEX NON-CORE SUBUNIT NAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31633:SF1">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX NON-CORE SUBUNIT NAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04410">
        <property type="entry name" value="Gar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="352"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="417"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="491"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="557"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="63"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="76"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="122"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="138"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="174"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="198"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="426"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="491"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="521"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="560"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2077883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="606" mass="68398" checksum="F364D6E25C17F8AC" modified="2022-05-25" version="1">MDDIQSLNTCPENDVKQESIEQQLEQPKEQEVQQEQETQLEGQPKEQETQLEGQEQPKEQEIQQEQPIQQEIQQEQPKEQQEIQQEQQKEQEQPQETQLEEQQQEKIEKNQQQQIDVEQIETADNSTEISANATEVVSIEKTESRENKDKKEEKESDSDSSSSDSDSDSDSDSDSSSSSDSDSTSSDDDLDDIVAPVNKSNNNNNNNNKKRKMDDEDDDFSAPKTKNEIELPKEEAIYTEIPKDIPLNHLGVLAHIVDDVMVIKSLQETPSALDIDSIVCLEDRTAIGRVFEVFGQIYNPVYSVRIPENLDISIKNNNNTKSNVVNKVKATEATVTKETDQIENNQDIKMETDSTPATEPTVTETLTASESTTAVTTESTITEPTTVAETIVAESTTVTAESTMEIESINNNIITGESKETKVESENQASTTEATGSSSREPAISAKKKILLDANTNIFYVNQPDLVKFVIPSQIYTKGFDASNEFDEELDENQMEFSDDEKEREAKAKRNNKKKNKRNNDNNNNLNNNNNNNNLNNNNNNLDVLENLNSFVNYKPLSRQQPSQQQTASTTTPTAPSGQNSQQKQIFDSYQNLMQMYQKQKQQQPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZF5</accession>
    <name>A0A8J4PZF5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000066">
        <property type="protein sequence ID" value="KAF2076295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZF5"/>
    <dbReference type="OrthoDB" id="7600006at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF180">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="667" mass="76352" checksum="478CCB9479C8E1A9" modified="2022-05-25" version="1">MDNFLQLYRNKSIKSNIYQYLLENVTIGCTLKYFLKYHGLFNHCIDRVELMVYQHDFVKYKDQMIQLQYKKCLKKVIFDQYFNEEFPVGFLPPCREIEFGEDFNQKLQIGSIQEGLETIQFPRVYSRALAEGVLPQGVKVVLFSFYPTGDHSSTSIENALPSSVERLSFYTNSLQLAACQNIQDLRIRNVNIAQVLWPPSLTRLECSIPPRSVVNWVPSTVLHLSMTETEYGENLEDSPVIELLPPNLVSLQLAGQFDAPFHSGLFPLSLESIDLTNSTFNQPLEGLLPVYLKKLILSYSFNHRIFPGDLPSSLEYLEFGYLFNQGNLSPIDHPNLFDQVFPQSLLSLRFGYDFEHELTVAMLPRSLTYLQVSRGSNHPIRSGVLPATLTRLKVHCSAPIEKNVLPPNLKILEFLDLFNQPLIPGSIPDSVEEIYIGGRLSTSRFHQSIEPGVIPPSVKKLYWYSSVFLHQRVKVSIPPTTDFKCFTQFNYGAPTAPLANFIYSSKIKYSIQIPKGFYSGESLKRLKFFNFNDTNYPLKQGDIPFGVEYLSLRDYTANPIKKGLIPSSVKVLALPNYSPTIFNNLPKSIKNIIVYRHLARYYDPEQEINQNLFEIDSIPSSVETLIIEAHPRINISDYYSGSERSVIYHVNQYVNDDKASYFDFIGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0R8</accession>
    <name>A0A8J4Q0R8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glycosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_001627</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000040">
        <property type="protein sequence ID" value="KAF2077060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0R8"/>
    <dbReference type="OrthoDB" id="411524at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042285">
        <property type="term" value="F:xylosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051292">
        <property type="entry name" value="Xyl/GlcA_transferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12270:SF52">
        <property type="entry name" value="GLYCOSYLTRANSFERASE-LIKE PROTEIN GNT13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12270">
        <property type="entry name" value="GLYCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13896">
        <property type="entry name" value="Glyco_transf_49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="23"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2077060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="622" mass="73419" checksum="AE1AEB76BD84A7CB" modified="2022-05-25" version="1">MGKKPYQSSGLQFFTKLSPKQKILFLCVGIVLIFIFLQSRIDRRDVKKTSRAVLRNYHDSLPNSEYLTKNILVDADFNTSVNGTNAAWRTLHYCCRSQNTFSSTHKETFYILQSYDKWEGLFQPIDINTLSKHYKRDHIDNTKTKYHMFHFEVDYINYNHEGNMILMFRGLLEDNTTLDLDSRITVENKGTWIKKISAELSRKGAKYYLGQKTSRVIDADTQRISLTLYSEKQFQYIIPYVVIYEGGTIILRNSSMSYGETNELETGAMDETKEFVGDDGGHPSHHKSMLVPTYLTEPVRKIVPYFRKAKFIHLNNDISIVSQLDISRLDRLTEMSKNWDGFISVAIFIKEDSQVEELEALLELPEHESLKGNTNIHLVYKNPTDKTYPINYLRNVAIDHSPTDLVYTLDVDFITSKHSHSLMKKHILKSEYFTTRNNSLFCVAPFEIDGYDMEHTDLFPETKNELLEMIKQKVARPIHFVKAPEAHRAIQYKHWEVANRIYRNYYEIFWEPYCVFNKTTTLQYDPRFSGYGWDKVSHAYHLYLFDYQYFTVPDVFIAHLDHPLAPWIKRTNEENLQIWINQYESMVHLQYKHYHSKDTFKNFRFINIMNNDVPFPEVEKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2G9</accession>
    <name>A0A8J4Q2G9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">O-methyltransferase family 2 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_001160</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000026">
        <property type="protein sequence ID" value="KAF2077542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2G9"/>
    <dbReference type="OrthoDB" id="16076at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000407">
        <property type="entry name" value="O-methyltransferase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712:SF2">
        <property type="entry name" value="O-METHYLTRANSFERASE CICE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_4G14580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005739">
        <property type="entry name" value="O-mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="6">
        <location>
            <begin position="20"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="5">
        <location>
            <begin position="118"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR005739-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2077542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="349" mass="39422" checksum="DB87C34847EDFF28" modified="2022-05-25" version="1">MISIQDDEQIRLDNERFLLDVATGHWKSRALNFALEAKIASHLVNGSKSAFTIAKEIGVNPEFLYRTMRALSSLGIFREEEEYGVFSQTPKSAIMAESSPSWTDGLLFESHDFTYKAWTSFRESVKEGKAMGPQSLGYESVWSLTEQNPSYAPILQRAMSSFTAQAINTIIRETDFSPFETIADIGGSQGVLLLNILKNNPSVKKGYNFDLPITIENNKSLVSQRESEFKNEMAKFEDVAGSFFDSVPEADCYTLKYIFHDWKDEKCVEILETISKSMKPNSKIYIFDSVIETKNQPNGFVWLDLHMMHYVDGRERSATEWKALANSAGFKVESVVSLGMIGRVILSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2N7</accession>
    <name>A0A8J4Q2N7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cell cycle checkpoint protein RAD1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_000475</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the rad1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000009">
        <property type="protein sequence ID" value="KAF2078185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2N7"/>
    <dbReference type="OrthoDB" id="337581at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030896">
        <property type="term" value="C:checkpoint clamp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003021">
        <property type="entry name" value="Rad1_Rec1_Rad17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10870">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10870:SF0">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02144">
        <property type="entry name" value="Rad1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01245">
        <property type="entry name" value="RAD1REC1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2078185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="324" mass="36204" checksum="2E304D49ABEC5D09" modified="2022-05-25" version="1">MSNYMNDVPMTPGASASMGTAQDTEVVAFLAKTENASDVSTVLATIYKSDPTDKVNKKTDIQAVQQFVTVKISEIGMSFIVEEGKHFQGQVFFKRSIFQEYHCQANSSAYVDHQSVVPQMPSFQFKINLAMILDCLNIFGHTPNKYTSLQFVYRGYGSPFILLLEEDGVITNCSIKTMDAEDTLSFDYAGTVANRVLIDSDNLLEAFGELDYSSSILKILLTPDIPFLKLSTNGQMGAFTMDFCKSREPGEIFESFQLNEPIRHSFQLSLIKPCIKALTIAKKTRMLLTRNGLLSFQHVVPIGTHVHTIEFYILASDLPDDMTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3G8</accession>
    <name>A0A8J4Q3G8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutamate-rich WD repeat-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_000618</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000012">
        <property type="protein sequence ID" value="KAF2078067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3G8"/>
    <dbReference type="OrthoDB" id="2161379at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051972">
        <property type="entry name" value="Glutamate-rich_WD_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022052">
        <property type="entry name" value="Histone-bd_RBBP4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45903">
        <property type="entry name" value="GLUTAMATE-RICH WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45903:SF1">
        <property type="entry name" value="GLUTAMATE-RICH WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12265">
        <property type="entry name" value="CAF1C_H4-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Histone-binding protein RBBP4-like N-terminal" evidence="8">
        <location>
            <begin position="77"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="283"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="326"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="372"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="145"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="11"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="152"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2078067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="473" mass="54051" checksum="E8C826D1DF81E003" modified="2022-05-25" version="1">MAEKKRNFETIDFEEFKEEDEDFDNDEEIDGEDESFEDEEEHSFEEDELNNDEDGGDGEEKGPKKVWRAGVDPLAEDEVLDYDSSAYDMMHTMSVEWPCLSFHPLKDNLGTNRKKYPHTMYMVAGTQADEAKNNKIYIMKASSLHKTKHDEDDSDEDNSDDEDSDDDEENDKDDDPELSINYVHHQGAVNRIRSMDQQQGIVATWSDNRSVYIWNVENYIQALENNTSAPKNTAPIHTVTQHTEEGYAIDWSPKVAGRLASGDQNIFITNAVGSGWKTTAQAYKGHTAAVEDIQWSPSEENVLASASSDQTIKVWDIRQHKPAISIKAHNSDVNVISWSRNIEYLLVSGCDDGSFRVWDLRAFKSNSPVSDFKYHTGPITSIEWNPYDESQVIVSSSDDQITIWDFSLEEDTEEFKEANDNQDDDFQYPPQLFFIHQGQSDIKEVHWHPQIPHVAISTARDGFNIFKSSNSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3P3</accession>
    <name>A0A8J4Q3P3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phospholipase B-like</fullName>
            <ecNumber evidence="9">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_000477</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Putative phospholipase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the phospholipase B-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="9"/>
    <dbReference type="EMBL" id="AJWJ01000009">
        <property type="protein sequence ID" value="KAF2078187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3P3"/>
    <dbReference type="OrthoDB" id="443524at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007000">
        <property type="entry name" value="PLipase_B-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12370:SF4">
        <property type="entry name" value="PHOSPHOLIPASE B-LIKE PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12370">
        <property type="entry name" value="PHOSPHOLIPASE B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04916">
        <property type="entry name" value="Phospholip_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7 9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035337718" description="Phospholipase B-like" evidence="9">
        <location>
            <begin position="22"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2078187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="546" mass="61977" checksum="BDC58F8DEA51F84B" modified="2022-05-25" version="1">MIKRSILSLLLFFVIAVVCQTNLSTYSVRISNTGTGGFQVYAGNDSNAVAQASYANEMMTTGWGYISITTNPSFDDTTMAQAAGYIEGYLSQELIWATWSNTYVNEYNSAAVPDNILNFATENMVYIQQQIKANPTDPYWTHIYLVWSQMSSMVDGYNDATTDSSQQLSLMEFMLMNMDADLGDIIPSLNLTVSDFRKERAIMKGHFKKTDHCSGLIKFTDDLQELYTAHTSWSPYYVMVRMFKSYNFKYSTSSKAHVMMFSGYPATLASCDDFYLLDSRLVVTETTNGFINNELYSLITPNSVLSWMRSIVSNRIAKDGLTWCQTFSRENSGTYCNQWMITDYNKFQPGVKLHDGTLYILEQIPGYIEYADVTGVLRTGYWPSFNVPYFENVFNMSGFNDTSSSDYEPWESDPRSQIFRRDANKVQTLQDFQAIMRYNDYQNDPLSQGDAANQISSRYDLNLPTASDYDAFGGIDSKVTSLALLNQLTVLAQSGPTHDQQPPFEWSNGNWSAMYPTIGMPNTWDFGWVQFTDTTFTIPQSSNKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3Y9</accession>
    <name>A0A8J4Q3Y9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial pyruvate carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_000971</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mediates the uptake of pyruvate into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial pyruvate carrier (MPC) (TC 2.A.105) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000021">
        <property type="protein sequence ID" value="KAF2077724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3Y9"/>
    <dbReference type="OrthoDB" id="869189at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006850">
        <property type="term" value="P:pyruvate import into mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005336">
        <property type="entry name" value="MPC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03650">
        <property type="entry name" value="MPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2077724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="139" mass="15418" checksum="84E124A61609BD62" modified="2022-05-25" version="1">MQFVRKLLEKYIGNTVVFNGPRATAFFARNPKLEFLNNVTNLAPMMKWSLSIVPISQIISGTKPPQTIDLAQASSLCATGTVWTYYATLIQPQNNGTRLLAICNGAMAMCHGYNIYRKTTHERSIKNPLHVNTTAVATD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q441</accession>
    <name>A0A8J4Q441_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000194">
        <property type="protein sequence ID" value="KAF2073601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q441"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="112" mass="13341" checksum="81CFCAA6654F0A91" modified="2022-05-25" version="1">MCSFDQDLNQLDSNQLFTMSSYKHTRSNWTQYQDCPYSLENGYREYFTKREYCNRFQSCETDNDCLKQCFQYCWQCWGGEQCNIDAHHGNTVSLNEYNCFFPGRGIIGNKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q474</accession>
    <name>A0A8J4Q474_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000237</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000004">
        <property type="protein sequence ID" value="KAF2078487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q474"/>
    <dbReference type="OrthoDB" id="24381at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="529" mass="60568" checksum="D713524AD742DD32" modified="2022-05-25" version="1">MNTFYLIWRNQYIRKLIRNLVCRDQVICVNDGYLNDNHQYLSVFTDKDKIDYNISIRFIDNSEFYFKNQHRSIINDLDFYLYSPDRVNCNHVDFNQFTGFELHRLSLQINCNTVGSGPLPESLTELFLDTSDYSESAPIVLHILSHLPCNLKTLKIPIEIIISEPVVVPDSLTSLEYYGKYESLQWVVVPPNKVFSGCQLQIDSVGALEWLKENKWVDTIYFYNYIQLDSNTMIPDHVRSIQDRGNVNLEQHRLPSMLQELICRETIPSQLSHLKILTLTHPIAKLEKNQLPASLEHLDIHYSQPLDAGVLPPNLTKLYLYTFDQILGVGVLPPNIKYLNMPVFNQPLMSSVLPTNLLELSIYSFDQPLLPHVLPSNLQILRMLIFDQPTLPAHSLPNSIVELYLNSFKGSILCKPLDHLKRLQLHSLHQSVSTLLQNVKRLSLKLQKTNDVLGTCLANTRIEKLCLDCHSESCLYPNSLPPTLKSLTLSNIDIQAKGTIPNSCVDLTSLDRELNPAFIPTSVKYSLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4I5</accession>
    <name>A0A8J4Q4I5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cell division cycle protein 123</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000531</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDC123 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000010">
        <property type="protein sequence ID" value="KAF2078147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4I5"/>
    <dbReference type="OrthoDB" id="16522at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009772">
        <property type="entry name" value="CDC123"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15323:SF6">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 123 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15323">
        <property type="entry name" value="D123 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07065">
        <property type="entry name" value="D123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="92"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="371" mass="43768" checksum="0FF86E9C1B9257C1" modified="2022-05-25" version="1">MNKTDKYFENKRKCMFQEWYPTFKQVTFSSTIIPLPKIFIDYLNSDHFVSNEQEFPQYRNDDPEFLDDESWSTPRRSTIVDSKYYQSYGSDSDSDSDDSDDSNDDDKKKNSRIIKESEFQHLLDEIKSIITKYGGYVLPKLNWSAPKDATFMNAFCTLKCSTPTDVFLLLKSSDFINHDLAQFETNEQDDDLTITPYVLVLRKWQNLTESMEFRCFVKDKKLLGISQRDTSTYYSFLKDKKESIQESIESFFNQNIKDKFSQDSYTFDAYVTKENKVWLIDFNPIHPSTDSLLFDWEEFFPELIVDDEESTPISEQDKLKLIQDLEFRLIENREGIRPNLAMESRLPMDLKDSSQSSAINDLLEKFKDKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4U7</accession>
    <name>A0A8J4Q4U7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase S9 prolyl oligopeptidase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004721</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000172">
        <property type="protein sequence ID" value="KAF2073976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4U7"/>
    <dbReference type="OrthoDB" id="449091at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42972:SF9">
        <property type="entry name" value="PEPTIDASE S9 PROLYL OLIGOPEPTIDASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42972">
        <property type="entry name" value="TOL-PAL SYSTEM PROTEIN TOLB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035221967" description="Peptidase S9 prolyl oligopeptidase catalytic domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="926"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="926" mass="101960" checksum="885C0BC64060E362" modified="2022-05-25" version="1">MKLYILLLLLLLSSISAIDYFDSTFYLNDWDILAPFPSAPREDVDVLSAFGGITNIPRADQSTYPSDLVNGGKTGWIKILNNNVNIVYNETTQVNWNLIESWAGESGSYFSGWGLSDFTLEASQMVVVNCVGVAKYFVDDMPMQGDTYGLGTSASSIYLEQGNHTVRVRIIGQEGASYQCSVSLISSSAAAAGLMILDSNVVVPDIVNGEFASPYVSIVALNLLNQTIDTNDMEVIVIFDPQQLVSNIALFKSGDDSQGSLQRGQINPINMVLKSNNPVNCTDGQTYSIGISVSTGANFGQVPFTLNFSCKSFGSAYTFTFVDFDFSIQYADATPPLYPCNNNPCPVLLTLHGASVDAKSNAWTQAYQRQNYSWTLFPTNRSPYGADWQFSGLKDAFSALSNLASELPGVPSSLRLQYQADPYRVIYAGHSMGGHGTFEISTHYPDRSLAVAVAAGWIDMQTYTPFFLRLGDSLNDPYVRFILDASISEWNTDALTPHLTNLPFIVRMGSIDDNVPPTHLRRMNRLYDQLNHNPTLGIVSEIPGEGHWFNGVVDDATMQAFFDKHVLAGIPPLPSTFMVTTLNPGSFEGKGGISILQLVVPFRVSKIQVTKISPTLWTLATENVKRFTFYAPYFTEAPNSVIVDGQALKIARLPNHYCIADDYTHYKSLSPKPRGIKAPAKWTICTDPSWQLTERSPLNSGPMIQILQYPLLIVYGTTGTASETLVRQNMAVYLANVLYYQLRYAITVLSDKEFTMESLSQFNVILFGGPTTNAVSKSLESNLPVQFNTNNNGFSINDNQFSLNSTGILFLSSCYSSKSTSTGASSIKTNKQYQNKMKFNQDDANNDGAPQRAGCMVSVIEGTDQNAFERAVMLFPTKSALSVPDYIVLGKNSDYLGSAGYLALGFWNNYWEYQDNLGYITNILNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q6G9</accession>
    <name>A0A8J4Q6G9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Aurora kinase</fullName>
            <ecNumber evidence="9">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_003751</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="9"/>
    <dbReference type="EMBL" id="AJWJ01000121">
        <property type="protein sequence ID" value="KAF2074966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q6G9"/>
    <dbReference type="OrthoDB" id="377346at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14007">
        <property type="entry name" value="STKc_Aurora"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000235">
        <property type="entry name" value="Serine/threonine-protein kinase ark1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="60"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="41"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="137"/>
            <end position="139"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="186"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="8">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2074966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="335" mass="38485" checksum="6F46606246B9FC42" modified="2022-05-25" version="1">MFSNKENSTSTVGAKNYHHQQQQQQATSSFKAPQRVLKPVNNNNNSNNIPSTASVKPSTTATTTTIATTTTSTAAPSSDKSKEKKRWVIDDFDIGKPLGKEHQLRREIEIQSHLRHPNILRLYGYFYDEKRVFLIIEFAKGGECFKELQKVRRFTEEYAATYSLQIADALRYCHSKHVIHRDIKPENLLIGINGEIKIADFGWSVHAPNSKRSTFCGTLEYLPPEVVQSKDYDQTVDVWSLGILIYEFLYGSSPFNSEKEQDILSKIKDNHYEFPSDVYVSDEAKDLISRLLNSDPYQRISLEETINHPWIKRFAHKYALEPTKLNMPIPSQSMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q8H8</accession>
    <name>A0A8J4Q8H8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001993</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000051">
        <property type="protein sequence ID" value="KAF2076736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q8H8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="124" mass="14185" checksum="9AF012B0B5E8C2E4" modified="2022-05-25" version="1" fragment="single">NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNSNNKKNNSKNKFTFDESDGERYDGESEDEGVDILDSNKKGNRINGLKRKTNTSHNFKQQSKKTKQNPNNTKHSYPGNSNVVRSNNIQDYLIVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4QAC8</accession>
    <name>A0A8J4QAC8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Anaphase-promoting complex subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_000751</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the APC5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000015">
        <property type="protein sequence ID" value="KAF2077951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4QAC8"/>
    <dbReference type="OrthoDB" id="2504561at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037679">
        <property type="entry name" value="Apc5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026000">
        <property type="entry name" value="Apc5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12830">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12830:SF9">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12862">
        <property type="entry name" value="ANAPC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 5" evidence="8">
        <location>
            <begin position="248"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="166"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="169"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2077951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="813" mass="94196" checksum="D5F3497E746E0977" modified="2022-05-25" version="1">MDKYRITPHKITLCILTEYYLNGRIHYHQKQSLSHLLINNIKKIDDNIDEISLSTFINTELQNILSSHFLNNEFLEMLSFESVDDLFSFITSLKDLFHSSSSNENNGNNTQQQQQQQHLLDPRSILGMFVKKIILNFSQILFPGLGKLYDQMAFYVNQFYRDTGRLRDEYDDDEDEEYEEDEEEEEEEEEQEEIVPNKVGFLSPLDEERFVYEQVIRLESRVNKEHPERIGQEIKNLKKLLPNVNRVHYIAFLNHLAHHDYEYSLEELHRYFDYSNSHSLNNDKTNAMLPYAVLNLAQLDYHYGHYENSFLALCEAIRIAQERSDHSCLAIAENWLGKFLQPSIVKSMQLSSGGSRNDNGFLVSQNHSEILTKSITRAHNVEMPDLLALNLNNLSSQRIFNSNNNNNNNNSSNNTIWTDIFQPLQDSSLLVTSQINSNMVNSISTTAWELLGNVDLSSYFTELVLKSSFNSNNELSLQNPISLGTSIGGKQSQASTLSDVTSLCRMALLKAKKDDFKGAISTLLTCHNIFKYHRLVNTTVSFTTLLVLYDCCSMEIINSSNWLIDLVLSSNNNNNNNNNRSKDYLLEIIIEQMVSLTDKFQPDDDGQGCGWLFIINVYEKISRYYIFEKQCFEKAYRFTTKAIQIAKTYGYHYYIAHFHSLLAKVFQQVSPPYSFVGLSNTLSSVSLSQQYNNHWLISESNLDLIQIHLDANQFDKASLLIQDTETFVLNNFKHCCTFYLLKSKLLIKSNSNNSKEIIKYLDQAEMYASKLEITLTNLYYKEIFYLKSCLYNSINDTEKRNEYALKFKLIINT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4URC2</accession>
    <name>A0A8J4URC2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Squalene synthase</fullName>
            <shortName evidence="5">SQS</shortName>
            <shortName evidence="5">SS</shortName>
            <ecNumber evidence="3 5">2.5.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_006731</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the condensation of 2 farnesyl pyrophosphate (FPP) moieties to form squalene.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>2 (2E,6E)-farnesyl diphosphate + NADH + H(+) = squalene + 2 diphosphate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:32299"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15440"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>2 (2E,6E)-farnesyl diphosphate + NADPH + H(+) = squalene + 2 diphosphate + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:32295"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15440"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the phytoene/squalene synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.21" evidence="3 5"/>
    <dbReference type="EMBL" id="AJWJ01000323">
        <property type="protein sequence ID" value="KAF2071956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4URC2"/>
    <dbReference type="OrthoDB" id="431150at2759"/>
    <dbReference type="UniPathway" id="UPA00767">
        <property type="reaction ID" value="UER00751"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055056">
        <property type="term" value="F:D-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051996">
        <property type="term" value="F:squalene synthase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045338">
        <property type="term" value="P:farnesyl diphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00683">
        <property type="entry name" value="Trans_IPPS_HH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000023">
        <property type="entry name" value="Squalene synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002060">
        <property type="entry name" value="Squ/phyt_synthse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006449">
        <property type="entry name" value="Squal_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019845">
        <property type="entry name" value="Squalene/phytoene_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044844">
        <property type="entry name" value="Trans_IPPS_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033904">
        <property type="entry name" value="Trans_IPPS_HH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01559">
        <property type="entry name" value="squal_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11626">
        <property type="entry name" value="FARNESYL-DIPHOSPHATE FARNESYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11626:SF2">
        <property type="entry name" value="SQUALENE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00494">
        <property type="entry name" value="SQS_PSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01018">
        <property type="entry name" value="Squalene/Phytoene_Synthase_Lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01044">
        <property type="entry name" value="SQUALEN_PHYTOEN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01045">
        <property type="entry name" value="SQUALEN_PHYTOEN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU368088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2071956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="416" mass="47437" checksum="BE7A6E9595668410" modified="2022-05-25" version="1">MQYVKSLTYPDEFLSLIKIGYTESFKPKSTLKDNLNNKEWCYEILNKTSRSFAFVINELDPHLKDAICIFYLVLRGLDTVEDDTTVAIETKLPVLTQFSEILYQPGHKVFGYGLNNDEKLLAENFDKVIDVFLKLGEGFCPIIADITKRMAYGMAEFLQKPVITLPEWDLYCHYVAGLVGIGLSRIFHASGLEGEWFATADHESNQMGLFLQKTNIIRDYLEDINENRIFWPRDVWSRYTLHLENFKNVKYQMSALHCLNDLITNALGHAVVCLDYMSRLKNEKVINFCAIPQVMAIGTLNACYNNYNVFTGVVKIRKGQRALIVDCIQNKGITGTYELFYKFANEMQSKVPVNDPSAKKTLEHLNAIQTLCVDKLGYKPSGFNDFISYDWMAVASVAASSAFLIARHGPNFFSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4US82</accession>
    <name>A0A8J4US82_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_005383</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000213">
        <property type="protein sequence ID" value="KAF2073306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4US82"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051210">
        <property type="entry name" value="Ub_ligase/GEF_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870:SF408">
        <property type="entry name" value="OS09G0560450 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035158112" description="RCC1-like domain-containing protein" evidence="3">
        <location>
            <begin position="19"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="RCC1-like" evidence="4">
        <location>
            <begin position="29"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="130"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="188"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="254"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="308"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="370"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2073306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="470" mass="51999" checksum="DC861B3BD3658F78" modified="2022-05-25" version="1">MNSKIILLLLCSIVIILFLKNYNNNISFVNGENDNDETVNYTQEQLQELLEAEIAAQEQPLPTQLLNITAKDIYANSYHSAYITEKGQLYHWGLLANSVTETPEIITIDGKEIEKVSLGGHFLLIKTRDGLLYSMGRGTYGVLGLGQDGQEVEKDYDLPQLIQYFIDNDIKINDISSGFEHSIVSTEKGIYSFGRTNKGQLGVGRSNSYSQEDNNNDIEFISTPQRIDNKVFESNNIVKICSGSDHNLVLTKDNKIYSWGNGYYGSLGHGDNKHLDTPKEIEYFNINKKQIRDIACGGYHSLALTEDGELYSWGWAELGQLGLGSNVTGNQLVPTRIQNTGLLVDSTEIEKITSIQAGAYATSFYLTDKGSLYSFGWGEGGSLGLGDGRNQFTPVIVAIPSEAVSVAAGFKHTIVKDIDGTIFVFGDDTYSQTGSKQNLNYKQVAMDLHPNDATLHTTNHFTHEQIKALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4USF3</accession>
    <name>A0A8J4USF3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">THH1/TOM1/TOM3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_005131</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant tobamovirus multiplication TOM1 protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000197">
        <property type="protein sequence ID" value="KAF2073546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4USF3"/>
    <dbReference type="OrthoDB" id="19798at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040226">
        <property type="entry name" value="THH1/TOM1/TOM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009457">
        <property type="entry name" value="THH1/TOM1/TOM3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31142:SF2">
        <property type="entry name" value="THH1_TOM1_TOM3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31142">
        <property type="entry name" value="TOBAMOVIRUS MULTIPLICATION PROTEIN 1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06454">
        <property type="entry name" value="THH1_TOM1-3_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="239"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="THH1/TOM1/TOM3" evidence="8">
        <location>
            <begin position="88"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="48"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="51"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2073546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="318" mass="37433" checksum="4CAC50029C287A96" modified="2022-05-25" version="1">MTDVLLRHHWLYENYDIIHFIVSFLYLMLSFFCVYSFIKRPNKTRQVLSHNSNSNNSNNSNNSNSNSNNNTQSNTNTNNNYNDNIIVRAVFMFLQPFIREQEFIQIPNQINILLNSLPSFNFFTIYLVVLFIWIEIFFLFKGSVASIKRLPTIFNTITILMYLCLFALYIIDFTMYPLESNPISAFITTPELVIGYYDASCFFIASLLFVFFGLNLIFRFRNPHQKNFTEEKRKIILKRIAGLTILTVACFICRASITLFALIQMNAISGLWWFDAVYYILFEIIPLWIMIYLLRMDKNRIRESQDFSNEKSALINSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4USF5</accession>
    <name>A0A8J4USF5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005146</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000197">
        <property type="protein sequence ID" value="KAF2073561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4USF5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="117" mass="12910" checksum="0C8FCB48129A570D" modified="2022-05-25" version="1" fragment="single">MDIGLPLALVVVVGVGYYLFHIFIVKPDSAFKGALEKPSTYTLSNTNSKPKKQTKPATKPVKKQQPKPEKKNPTEKKVEEPVVVAPVVEKKAEQPKPVEQKKAAEQPKKAVEQPKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVE8</accession>
    <name>A0A8J4UVE8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_010095</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000921">
        <property type="protein sequence ID" value="KAF2068580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVE8"/>
    <dbReference type="OrthoDB" id="19923at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 68F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808:SF9">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN GACX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="4">
        <location>
            <begin position="114"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="308"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="451"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="602"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="681"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="52"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="308"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="335"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="376"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="451"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="492"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="603"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="681"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="692"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="708"/>
            <end position="725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="859" mass="94101" checksum="6A8B4B3B5B514B0D" modified="2022-05-25" version="1">MFGWKNKPTTPTTSSPLTPSPVSNLAAAGGNGGTNNNNTGTNSPVTGPPVSSPTSSVSGLGPLGTTSNTSSGNLGHLVSPSNSSNNLTGYESIGRNSIDPSLIAANNVNALFGQAINPNLESGPPILLKFTKYLVTKLPDNIFKPHPEFDNDVQNLKKNLIKDISIGDIDQIQLPALTQNPFVVAEIMKQYLYALPEPLFSFQLYDSFLLTHSILLPIDRIWAYKYLFDNSLSKGYKGAIRSVLGLFNKIHESASTTKMDSVALSQLFSSVFLRPEEEMYYMKSDKSTIEEIVRLWIEEFETISKPSINPNQKVTTNFSTPPSSMQQQQNEHPLSSGSVVSSNSGVGMQQQQQQQQQQQQQQHPTIGMNKVNPIRPTSVGTPVPPLVTLLPTGTASLSSNINNTNNTNNNNSNNNIKSPKFSLPTIPKQNNNQQITVIKPKPTLQQPTIINITNTSNNNNNSNNNNNTPKNNSPIPSISTTPNISSPSLPSKSIQPLSPKSNTSNQLIVNTTLIKDLDQATMDKVNKIKLSIESAVSEQVFGLVKTTYVNIELEFKLAVIIKLTTVLRDAKKHIVESSEKLNITKTTVRDFLQSSPKPANYQLSSLKSFPSPSSSSSNDETEKKMNDLKRASQIAAEEISDYIYYYKAKIYTFEYKEQLVAIAQSLGKLKNILESGIGASPSSNNPLSFSSGGESPLNSPSTRRRSEILSSPSPKDTTAASFGSPTPNPILEQRSSTIRVIQVCFKEIQDRIQVMKTNLETATLVDTVEMGKSIRLTKQTLHELYIENSLILPPETKVSPNPNDDQLTNLKMALIPILDRILLQVDETSKIVFTDPKASEKEIQNITDKLLFVNKALSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVT9</accession>
    <name>A0A8J4UVT9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_009651</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000767">
        <property type="protein sequence ID" value="KAF2069030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVT9"/>
    <dbReference type="OrthoDB" id="1112565at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08368">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24205">
        <property type="entry name" value="FOUR AND A HALF LIM DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24205:SF16">
        <property type="entry name" value="GH01042P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="5"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="62"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="131"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2069030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="191" mass="21824" checksum="93D16C091DC178DA" modified="2022-05-25" version="1">MSSSKICKKCNEKIDGEMITVLDGDWHRNCFTCSTCQKPMTGFYNVGDKTICKECYEATCLKTCQKCKKSIVGQKLTDNLNRDFHPECFSCCTCSVKIDGSYFVLNEEVYCLKCHEEKRQKNKKENTKEMGSCFHCKKQILNTDGPMIVVNPNEKYHKSCLRCIKCKKEMEGEFIVADVGTHSYVCMACSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWR3</accession>
    <name>A0A8J4UWR3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_008692</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000561">
        <property type="protein sequence ID" value="KAF2069990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWR3"/>
    <dbReference type="OrthoDB" id="29755at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="4">
        <location>
            <begin position="181"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="136"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="79"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="195"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2069990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="265" mass="30524" checksum="407C36D191595577" modified="2022-05-25" version="1">MNGLLKRVDKIKKETVDANDKNKQLNADGTTDAFTLLRRKIGLDIKGTRELIKERDEAETSMPGSVRTVDLSHRIRVAFKEIETEIQKLQRMYEKAQDRYNKKNKQNSEKLKDIELQKEICQLAWAHFKELENQNRKRGGETSNFIPRDHQSSSTVKQLPDIDNDDFKELIRNDNQINKILDKIKDDLIVTGNIAKDMKKAAEGQSAKLDDLSRKADDLDTKLENINTRLAKMIKDVRKGDRFIIDVILICVLLGIGGFIYSRVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXP8</accession>
    <name>A0A8J4UXP8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S acidic ribosomal protein P1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_007473</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000401">
        <property type="protein sequence ID" value="KAF2071205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXP8"/>
    <dbReference type="OrthoDB" id="2194681at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05831">
        <property type="entry name" value="Ribosomal_P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000001">
        <property type="entry name" value="60S acidic ribosomal protein P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696:SF10">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="88"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2071205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="112" mass="11553" checksum="F93842216725965A" modified="2022-05-25" version="1">MAEIKTEELACIYSALLLNDDSIEITDAKIKTILEASHVTVASHWPGLYARLLAKTNIEELLLNAGSSGAAAAPVAAAAAAAPAAAAAKPAAAKKEEKKEESDDDMGMGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UY87</accession>
    <name>A0A8J4UY87_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TsaA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_006378</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the tRNA methyltransferase O family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000292">
        <property type="protein sequence ID" value="KAF2072300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UY87"/>
    <dbReference type="OrthoDB" id="4882at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09281">
        <property type="entry name" value="UPF0066"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.70">
        <property type="entry name" value="YaeB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2310.10">
        <property type="entry name" value="YaeB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023370">
        <property type="entry name" value="TrmO-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040372">
        <property type="entry name" value="YaeB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036413">
        <property type="entry name" value="YaeB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036414">
        <property type="entry name" value="YaeB_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12818">
        <property type="entry name" value="TRNA (ADENINE(37)-N6)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12818:SF0">
        <property type="entry name" value="TRNA (ADENINE(37)-N6)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01980">
        <property type="entry name" value="TrmO_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118196">
        <property type="entry name" value="YaeB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51668">
        <property type="entry name" value="TSAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="TsaA-like" evidence="3">
        <location>
            <begin position="68"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00033753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2072300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="355" mass="41016" checksum="F2CB3958C16C41FE" modified="2022-05-25" version="1">MFHLMLQRTRGLFSSSLSYRFYTSQIESSSTKPIVNHVNQQEEQEQKELKQSQYKKRISSRHKYRITFDPIGYFESVFALKNGTPRQGSLSPNSSGKVKIQCSNALHMLRGLDEFSHAWLIFVFHGNDRELIKPTDQLTKGPKKRIELNYNIHKEEGVLPAPKHVTVRPPRLNGDRRGVFATRAPHRPIPIGLTMCQIERVENDTVYFKGVDLIDGTPIVDIKPYIHLYDSQPNATIPSWVETKTEIESVEFTKEALENIEHCVKVVGLDMLKGTESSTLEEKIEKVKNVISEVLFNEPRSAYRRKKLPDEPWGFYIDTLNIQCQVKESVATVLVVEDKYDHYNDLTKSQYYRAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYU4</accession>
    <name>A0A8J4UYU4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase S59 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_005094</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleoporin GLFG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000194">
        <property type="protein sequence ID" value="KAF2073605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="21062at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044614">
        <property type="term" value="C:nuclear pore cytoplasmic filaments"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034398">
        <property type="term" value="P:telomere tethering at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1610.10">
        <property type="entry name" value="Peptidase S59, nucleoporin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037665">
        <property type="entry name" value="Nucleoporin_S59-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007230">
        <property type="entry name" value="Nup98_auto-Pept-S59_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036903">
        <property type="entry name" value="Nup98_auto-Pept-S59_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021967">
        <property type="entry name" value="Nup98_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198:SF6">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP98-NUP96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198">
        <property type="entry name" value="NUCLEOPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04096">
        <property type="entry name" value="Nucleoporin2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12110">
        <property type="entry name" value="Nup96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82215">
        <property type="entry name" value="C-terminal autoproteolytic domain of nucleoporin nup98"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51434">
        <property type="entry name" value="NUP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="5" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="8" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase S59" evidence="11">
        <location>
            <begin position="992"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="19"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="165"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="284"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="569"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="809"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="858"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="927"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1156"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1228"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="70"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="85"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="103"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="175"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="284"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="310"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="336"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="381"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="412"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="427"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="450"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="477"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="517"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="533"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="569"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="621"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="633"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="649"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="662"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="927"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1167"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1252"/>
            <end position="1267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2073605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="2019" mass="212899" checksum="1CFD64DBF67DB3CF" modified="2022-05-25" version="1">MFGSSFGGFNAKPAASTPFGAPSTGSSLFGAPQPTSTTSTGGFGGFGGFGQPATSTTTTATSSPFGGFGQTTTPATSSPFSGFGSQPAAAPATSSPFGGFGQTTAAAPATSSPFGGFGQTTAAAPATSSPFGGMGGSAAPTSLFGTSSSSAGGFGGFGASATPATSSPFGGGTGTSLLSGQTTSSSPFGGFGQQTTTTTTSSPFGGFGGAPAASTSSPFGGFGGATARPVGTGAPPYTATQSEGCSFVSITAMDAYSTKSFEELRLEDVLNKKDQIYKTGAAATTTSPFSTGGATSSPFGGAATSTSPFGGASTTLGGTTSPFGGASTTLGGTSSPFGTSATTTSPFGGASTSSPFGGASSTSPFGGAGTGLPAGSSASPFGGATAGSSPFGGASSSPFGNTGTTMGGGSSPFGTTTNTSTSPFGATASSSPFGAAPSTTSPFGGGSLLSGATQPSSGLFPSSTATQTSLFNLGGPSATTQQTQSTSPFGTATQTSSSLFPSSTTSTPNSIFGGLGTNTSQSTASPFNNTLFGQQPSTSTSLGGTGLGLGQTSATPGLFSGLGATNPSTVSSTSLFPSTTTSTQPSLSLGGGTSLWGAPQTSTTAQPSLSLGGGLGLGLGQTNPTSSFSLAPSTTFPSTSGSSLFPSSGGLGTGSQGGLGLNPSNTFSLSGTTTQPSNSISNMFAPQSTTTATPSSLGFGNTLGLGGLGTNTLGAFPNQNTLQVQTTQTPQLNNNIASQSPYYPIPSAAPLSNFVKSVTASQAPAPPPASSATVQRSLSHLSYVPKSSTKLVPRRGDLGFNLLPSNGTSSSTLFSNGSSSSSTSSSSSSSSSASNNNLFPIDKFITKHSKSLNINTTNETEDSLKQQQGNNSFPFKKDRKSNNNDDGGFYNQDNSVINYSSVGSNSTGSTYSSLGLPSNQYTSKTSIIGNGSTGSSINTSSSSTIPTTSISSNINNGKEQQQQPTTSTTTSSAKESKPQQQKPNPNAPRLTKEGYTCKPSIKELSMMSDKELSKVHEFTIERAGHGSVKFFGDADIRGLDLDNIVSIQDREISVYPDEHTKAPVGQGVNRPALVALERCWPTVKGKPNQYRKNPEDLNKYERALKKQAEVGEFKFVDYDQEKGVWRFTVEHFSKYSAPDDFDEDDVDQAVVEQQSRPITFQAPAPITSSSAATSSSSSTTTKPKFSANIAFESDVESDGNSSHDESDFIVPQKKYNKTPFIKRATTSRESGLFDEEPSVLKQNEESSHKVSKISSNPSLKVSRGPNSNVNVTSKSVYTIPPPTNFTVEPLTIPTGGAFKIPTSVSSKSTTASSVVPSPTTSSMEDVVVLSKPKIKIDSTILDRIVPSSESILKDYPAVKNTNQDPALFMRRSFRVGWAPGGRFITTSKGSLKKLIIHQIPTESDKKSKKDDILKFLSEHHSNSSLVPANQRAIGWFALSQVQNQIESQLTCSKLCKNADSVQWNDIDSYYHRIWSLISTLWGTNSKETEQFSMETTRKLKFNQWVKDVCVPLIKQDIEQLKKKPSSSYLDSIFTFLTGKQIKEASNLAYENKDFRFATMMSQVWSSDENGKLLLLKQIQDYQDKGIDNFINKHRIEILQLIAGDIDQVYKNVSDWIRCFALNFWFKFPLNSPLLASIENFEQSYLENKCKVPLPPYLIGGENNNNNQFYDICYLLIKLYASDRFDKYENIFYPQNIGNDLLDYYFSWNLYSVLKSIPSLNKQPDVGNQSLLHSSFAAQLERLGLWQWAIYVLLHTPDNSNHVREEAVKSLIVRYTPVMSGEDKSFLTQKLCIPQLWLDEAASWYMGYSRQDSNNEQQQLQQQQQLVDMLIKSHQYSSAHDIIFNSIGPKSVIQSKFYQLSLVLQPFLSHATSISSWRLGGAIFNEYCQINFNFQECVGFLSSLENQDELNYMKTILKLRELSDRISNLLMDVSKLNQSLDIKNTSDIYKIALSSISNNLLSKLILLKDIPSAPGQSTPLISTNKIVSNLHGLPLTQDFRSNKLESITNQIQDMFLTALY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZ70</accession>
    <name>A0A8J4UZ70_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006117</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000268">
        <property type="protein sequence ID" value="KAF2072568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZ70"/>
    <dbReference type="OrthoDB" id="21524at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2072568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="980" mass="114251" checksum="F40119FDD8938CEC" modified="2022-05-25" version="1">MSDEKESVFKRLDLIFNFLFQDTNDHQNNDDDHHNHDNHHSSDETRFEKIFDRLKSIIDSSNALFIEYLIENDVVARDIEIINNRYKKRALVTTTTDFEYSLLSANIRLLGLFFQDKQIYQINKDKIKQILEMMFQFLLVHENPLIRNRSLVSINSIVLSSGSSDSNNQQSPLFLLDLDTCSPRQCISTIFQSFQYPSTFVSHSATILMSTLFINSFKNNYSNNIEHDLPLIILKLVEESIQQQQQQKNETNLVGIKLNSDSLSVDTILCILDFMIQILEYLFATTIDSTPDQQQTSTTKVKIIEFFKSSNLVHKLSSLVLDQNRLVQSRAMEMISIISNFKSTPTTVDSLAIIDFFTSMKGFYKSIIQKLVLPLSEQTLNKSSLCTAIDLIGTLDNTFLFPLDKDSKQPLLFQQLNILVQILYSTISTTPNNNDISVPEEVYLFHIFQATKEILKKSNGDGEYPIFSSSWIEFLVFVSTLKAKFYSNVSVKTLKECLHCLKLLHIDQWISIFIQDNNSFNFNSNNNSSKQNSIYFLMKNQSKYSRFVLKESLDVFLENILPNYQYVRDHVTEFDSLFVDMLSSLLMNPSSDVRDQCLIFISNIFKNQQLHAIQDTLFNYSIGFMVLKKLVDSDNYVRSSTLDTIKIISTISYKGWSHLCEEKDESIFKNADQLHLIIKETPSLTINDDLCNNNQINLKNYTNLLEKERKESKNHLFYEDTISMGSIEDKGASNDLIQDEDWEWDDQEFKELCERNRQEPINFMQEYDNEINMNNDENSSSIQFIQESLNYKFPHSILLFLNDEDSYPRISALEVLISWLKESYSFEFLQSLINDPQENEKYRIQSIFTDLMGDNGYWEVKSKCIEFFESIYEKEISINNQELNMIQNLNVETLLLNNIISELEDKIIIMKSLALYQKILNWKKQIKNLNTTELQNKVDSVLNSLYDEFTEEEQEDIYLFSIEKNDNSNNKEDNNAIDCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZJ4</accession>
    <name>A0A8J4UZJ4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcriptional coactivator p15 (PC4) C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_004133</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transcriptional coactivator PC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000141">
        <property type="protein sequence ID" value="KAF2074550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZJ4"/>
    <dbReference type="OrthoDB" id="20677at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.31.10">
        <property type="entry name" value="Transcriptional Coactivator Pc4, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003173">
        <property type="entry name" value="PC4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009044">
        <property type="entry name" value="ssDNA-bd_transcriptional_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045125">
        <property type="entry name" value="Sub1/Tcp4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13215">
        <property type="entry name" value="RNA POLYMERASE II TRANSCRIPTIONAL COACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02229">
        <property type="entry name" value="PC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54447">
        <property type="entry name" value="ssDNA-binding transcriptional regulator domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcriptional coactivator p15 (PC4) C-terminal" evidence="8">
        <location>
            <begin position="75"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="22"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2074550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="137" mass="16171" checksum="55CCC8B63BAEAA9D" modified="2022-05-25" version="1">MYKGKKKFSYSKKAAPKRKVESDEEEEQEEKVEKEQEEQEEEEDETKTPKEKETGSSKKTKTQTSEVNEKEKYIDLGNKKRIGFSDFKGYKRIAIREYYEDKSGELKPGKSGISITFEQWALIKKHMDVIDSWTKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZN3</accession>
    <name>A0A8J4UZN3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">60S ribosomal export protein NMD3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_005351</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as an adapter for the XPO1/CRM1-mediated export of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the NMD3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000211">
        <property type="protein sequence ID" value="KAF2073333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZN3"/>
    <dbReference type="OrthoDB" id="203821at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039768">
        <property type="entry name" value="Nmd3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007064">
        <property type="entry name" value="Nmd3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048899">
        <property type="entry name" value="NMD_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048898">
        <property type="entry name" value="OB_NMD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746:SF2">
        <property type="entry name" value="60S RIBOSOMAL EXPORT PROTEIN NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04981">
        <property type="entry name" value="NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21193">
        <property type="entry name" value="NMD_SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21192">
        <property type="entry name" value="OB_NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nmd3 N-terminal" evidence="9">
        <location>
            <begin position="1"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="60S ribosomal export protein NMD3 SH3" evidence="11">
        <location>
            <begin position="220"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="60S ribosomal export protein NMD3 OB-fold" evidence="10">
        <location>
            <begin position="282"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="435"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="435"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="448"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2073333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="501" mass="58178" checksum="70BE3E27C3240F26" modified="2022-05-25" version="1">MCVECIKSQVDITEGIPKQLTIQWCRGCNRYLQPPNHWAYCELESRELLTLCIKRVKGLNRVKLVDAGWIWTEPHSRRLKVKLTIQKEVFTSAILQQVFAVEFVVQGQQCDKCQKFEAKDTWNSVVQLRQKVDHKRTFLFIEQVILKHNAHSQTLNIKEKPDGLDFFFSSRNHALKFVDFISAITPVKTKKSEQLISSDEQNNAANYKFTFSVEIVPLSKDDIVYLPPKIQHQLGNMGPLVVVTKVSNLIHFIDPQTLQVGEISALTYWNAPFRALSSYKQLVEFTVLDVNLSGTTRGRYALADVQLMRSADFGVNDNIFEIRSHLGNVFNAGDLALGYDVTSSVFNDQDLIGIKHNQYLPDFVLVKKTYPAKDDFRFNRFWVLKPLQKEGIENPKNSDLEKMERDQEDFAREIEENEELRSKINIYKRENASQLKADRDKKKKDMGLEENDLDDQIQLEDLLDQMEINDQDFDDDEDEDDEDYDGEDYDDEEEDDDEMMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZV1</accession>
    <name>A0A8J4UZV1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005143</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000197">
        <property type="protein sequence ID" value="KAF2073558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZV1"/>
    <dbReference type="OrthoDB" id="20755at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="143" mass="15811" checksum="339D82713DAB069E" modified="2022-05-25" version="1">MSTPPINNVVQPPPPQQPPLPTQSLEEITQNLRLFPMVLDIISRIKDDEIDLIKAINILNDRYYKSFDQLLKIPGIDRSLEEQETLLKSNKEALEKRKSNTQIDDTHIDVQNILTSSSSSSSSSSSSSSSSSSSSSSLSSSYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZW2</accession>
    <name>A0A8J4UZW2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010286</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001017">
        <property type="protein sequence ID" value="KAF2068387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZW2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="54" mass="5874" checksum="8CC40159DEB9A7F3" modified="2022-05-25" version="1" fragment="single">PKSKSLSAAKIAGIVIGSVAFAAIIVVSVVYYVYKKRQYGKMKRGLEQKLQSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0K4</accession>
    <name>A0A8J4V0K4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_002859</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000084">
        <property type="protein sequence ID" value="KAF2075825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0K4"/>
    <dbReference type="OrthoDB" id="272512at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063:SF60">
        <property type="entry name" value="LYSOPHOSPHATIDIC ACID:OLEOYL-COA ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063">
        <property type="entry name" value="PHOSPHOLIPID ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="35"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2075825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="349" mass="39408" checksum="5ECA84D95806A9C5" modified="2022-05-25" version="1">MEKFSNWRDGPTGIHPLLPTKSKNVNVFDYIFKYVIGPLFGILRLPFVVLLTLLLVLFDTLTRMIPVGVISRVLNRFFTIIFARWILFAMGYFKIEESTETLSRSKKAAGSIGTFKMNDVIICNHSSYVDIIYLAYRFSPTFAVPPNDRDPTTFSARLIPLNIAQALKNAIFSPLHLNQDSVDTKILMEKITNSWSGPLVILPEGTTSNGQGLLDISPVFESDSGIKSLDFIHVLGITYHYGMYSPCYSSVGSVWLHLFKLCSQVSNGIQIKYLSRSCIPPLPDLQAKSIIISSKSSSVVSELQEWFDNVFKALSSMINTRRTKINSHDKLSFVAYWKGYKVVYDKKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V161</accession>
    <name>A0A8J4V161_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003469</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000109">
        <property type="protein sequence ID" value="KAF2075208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V161"/>
    <dbReference type="OrthoDB" id="439743at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="175" mass="20278" checksum="A3367C3E6812E8E7" modified="2022-05-25" version="1" fragment="single">RANLKKIKYESSNQVFPSSVKCIKFGITFNHPIYRNTLPDSLTELYFGEAFEQPLEKGLLPSSITKLTFERGFYHQDLLDWIPHSVTKLQIGHIHTDQRKNERPWNIVFPIEKLLNTLVSSLILGDRETIESTDLIPSNIKEIRLCKCNIIKHIPTTIEALYLGNSHNEPLNKYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1E1</accession>
    <name>A0A8J4V1E1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SD-repeat containing protein B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_008283</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the serine-aspartate repeat-containing protein (SDr) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000497">
        <property type="protein sequence ID" value="KAF2070397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1E1"/>
    <dbReference type="OrthoDB" id="17084at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033764">
        <property type="entry name" value="Sdr_B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36108">
        <property type="entry name" value="COLOSSIN-B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36108:SF17">
        <property type="entry name" value="COLOSSIN-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17210">
        <property type="entry name" value="SdrD_B"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117074">
        <property type="entry name" value="Hypothetical protein PA1324"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035269993" description="SD-repeat containing protein B domain-containing protein" evidence="5">
        <location>
            <begin position="26"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="domain" description="SD-repeat containing protein B" evidence="6">
        <location>
            <begin position="38"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="SD-repeat containing protein B" evidence="6">
        <location>
            <begin position="202"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="SD-repeat containing protein B" evidence="6">
        <location>
            <begin position="864"/>
            <end position="954"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2070397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="981" mass="106365" checksum="FF4F19C640F46ADA" modified="2022-05-25" version="1">MRNKILLNVIILLLLVFNDRNFVKSDYNGFIIALKGVCYLDLNQNKLFDDGEPGFAGVAIDLANNSTDYTGAGIAPMVTKSDGVFVFDKLMESTIYEIRFKNPAGYLFEDHEKVLSCPEGKVCVRDSVAADHLVADKVIENTLAYNVPLIKADGAIQGTSIQVIKITVTFPHPTQPPTIAPTLPPTQPPTRPPVYEKGSAVVGNFYVDANGDGSQNSNEGYASGIAITLLNNNDKTRAIDAYGKQVPTATTDSLGKYIFDNLAPGVYCIWVESKSMLNPGCVGQIVVSVDGNGVSSGENSNASDGVRAAKIIRLGTYGAFPSGDSSVSGVVFSDKNLNNVYDSSDVGIPYLKVKIFLPVYNLFYQMTSTDSGGKWSINGLPSGFPFRVEYVLPDEFTSTGSTPVKFVVGGNNNLKLGLAYKNVTGDGSGLNFPRAFATSCFVKGPYNGANRAEPTLVSFTKDAIGKGYQSPGNSYMTKLATHKDIGSVHGVAYDPFTDTIFASAYHKTNSDFGPEGSCAIYKINKGQISTFVNLNNIFGNNNYCGGYHHTFDFREISVASSYVGKVSFGQLMIVKRFIYVTNLARNEILQIPLNNPTKDNIVTFAVPNPGCLSDSDWHIFPVMEYQGQLFTGGVCSGENGSKLSTYILKYNDKKKVFSTVLFFTMNYARGCRYLDTFANCISSQWVSWNTKSDNPQPMLSSIYFDDNGHIILNFKDRSGDISSTVSAPDMLVACLGGDGLYYLEKGGVCGGVVGAGTKQKSLAGVPYGPGGGQFFDLRNPGLHDYTASFGSTKAGPNMIVSTGFDYYDAFEGSIRWFNTSTGKTTKGYSLYISNQRSATFGKQNGLGDITGIYDPTISQFVVGRIWNDANKNGIQDANENGIGGISMFLISASNQNPINQIVSDKNGYFYFSVQLNQNYHCACKISDLPTGSGLSPVLTDNSNAYINSNAQKYQDIYIDSFKSESYGGFYFNHCHFGITYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V281</accession>
    <name>A0A8J4V281_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007425</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000395">
        <property type="protein sequence ID" value="KAF2071267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V281"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="396" mass="44674" checksum="C856923042AEDF5E" modified="2022-05-25" version="1" fragment="single">MSSISFFFQCLRNKCISRKISLYQRVVRGQFIRVTLPFVCHQIQDLVELGEYGVCVSLSVSRYDYKSYSQEFMMLSEKEKSMIKEIVFLSSEVMYTVDDGFIPMSVKSVTMESSFNQPITMLLFPSSVEKINLSNAYYFQHIIDIDQLPENLTELSLPRLAQEPFVNGPFSPSLKKLKIQGFLAPFNQHILSEGLETLELGPYKHFSSPLPSTIKTLIIPNINTIQNLPPSLTDVVSKCFGVDTQLPSTVEKLKYTQGSGFLHRFPTVPIHLRELECFVLEHYIDIDPGCLPKSLEKLSLFGFPGKIHPGAIGPNVSDLTLILSKKKTNFDDIHIPLDGGIKKMYLVVGELSKLPKSQIVPHSVTNLKLLSTDKQFVKDNIPKHCITLDLLDSPLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2G7</accession>
    <name>A0A8J4V2G7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000367">
        <property type="protein sequence ID" value="KAF2071507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2G7"/>
    <dbReference type="OrthoDB" id="21493at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="310"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="222"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="314"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="346" mass="38477" checksum="03B1DCCC7D818E34" modified="2022-05-25" version="1">MPIFQEDFNIDASSFNNNNNNNNHNNNNSINPFPATNNNNYNNNSLHYNYNDNEDTFHLKQTNNMDSSSESITTTLTNNSGANTRQNNSNSPNNGGFSPNYYESSPFHSSSTSTNLINHESTGGGFMEHWSSTSSPLASSFSPSSDLLTSSYLPSSSHSPLSPFSELQSDIYNSNSNSNSQQQHNHFKSNNTNPSYDGYFDHSNSFSILNNNNHSIIDQEFNNSNNSNNNNNTPPNKRSRLNNGSVNNNNTNLKSSSFICKTCNIKLQACYISFDEIILCCPTENCLFPLDDENLSSFILPSYLFKKRPRKLNKNNNNSNNSSSNNNNRAISISSPPTSSINHNNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2I8</accession>
    <name>A0A8J4V2I8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000359</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000006">
        <property type="protein sequence ID" value="KAF2078375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2I8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="91" mass="7717" checksum="E5D96A1A46A62D5B" modified="2022-05-25" version="1">MTIFGSLAKIGNPSKSITNGAFAASSTGNPNISNDIFANKGGGSGGGGDGGGGSGGGGGGGGGRGSRGGGGGGSGNGGDGGGRGGNTGGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V361</accession>
    <name>A0A8J4V361_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006404</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000294">
        <property type="protein sequence ID" value="KAF2072272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V361"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32556:SF18">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32556">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2072272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="399" mass="47460" checksum="64F3B414AD54B2B0" modified="2022-05-25" version="1">MPFIFQQSVLDIQSKLIYSVRSSLVRDLSLVCWRWFNYLSKLLSPSKDEFHKKKDRDSAIYCNYRLLHRKEFIKSLKIPLKLFDKNLVSNLNRYYTSLERLDIIVLDNDKDLSLLSLLNELNTHSTTNPAQKIDIKLDIYPPKNGKVLCDEKWLKKVEFTFTLTWFQFIYNSRKTNDNFNWISTLILKLCPRNITIIPENSGFKHLEFYHTISKHNQHYQSIRIENDFIPLFSLYHFIQSPNLTSFKFSLDIKLINELYSQPPIENLDYYLELEKDNRISEDQFQNRLPTVYAKSIWEKSIELLKCNSVITELYMYQLSGVHYTEPCQQFIQDFIDIFSNNKCIKKALLEFKFINNTVLKQILDNNTTLESLTIRPLLVNQIEELKSLTSNKCKIYSFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3J6</accession>
    <name>A0A8J4V3J6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000262">
        <property type="protein sequence ID" value="KAF2072632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3J6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="205" mass="23495" checksum="A75D16D7E9D45AF8" modified="2022-05-25" version="1">MFTTQPLSGSNSNSYSNSIGVSNSNSNSNSNSNSNSNSNSNSNSNSNSNKTRCNNHEQDIVYCTHFGANSNGFLVDIFCYECVKENGMDQIYSIKSNWDQYNTCHNQFNQIKGPRKALIETLIQIEEISVAQREVVLPFSLDPIDYYQFKKVNNFSLENLKERLILHDQEEKYFSKHIKTHRDLYDQIRSPPQPAKPFLFIGKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5H7</accession>
    <name>A0A8J4V5H7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004164</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000142">
        <property type="protein sequence ID" value="KAF2074537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5H7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053019">
        <property type="entry name" value="GATA_zinc_finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23353:SF23">
        <property type="entry name" value="PROTEIN HAIRLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23353">
        <property type="entry name" value="RAB-GAP/TBC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="56"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="761"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="111"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="761"/>
            <end position="787"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="827" mass="97719" checksum="266081029AF89032" modified="2022-05-25" version="1">MLKGGGRIINSTTARYSAANGSGGIRCPKSLSITPSSSSSLNRTYISVNNISSHSMGKDNQFYSSGSSNRNNNNNSNHNSSRYLVDQDQDYQNIIKREFIFQEEALDDEHLDLFESKRERQQRQREEEEVQFLDESEEVEDRYLVQLKRKQKQLQQQLDIDGNEYIEESQEWRQQREKYLESKEKRLLFTNKLEKIVSLESSTASTAATLTTTPPINQQQREEFNQLIKEHPNISTLLLSTQQLLTFLEYCCKYIKESKVIIDYFDQIPLDKTRFNPVLFDTFVRHMVGQKDVDFVEVFNSNIAYYEYYLSSGSFIDVLERLFVLNRLDEINSIVEYAWNNNLVSLVALNSIVGFYHKHFSIQQFPPLPYFASQLGLKFIQQHESIDQIFSVIKYYSSQNYYLSKEFYTELIALCKQQPVYEGQAQYLKKTIEYAFNCIDFGSILYYHQLYDQSTYLLLEAHLKKMAKDRQHTRKINAMFESIIRIQLKLYMYNDAIKSYLYLINDLNIEPSYEIIYNFSFYHKTNRQFSAAKWWYDKIKAFSLKPYCTDFELQHQYHNFSVPHHFKYFIRQNPQFKAYLDSSKGSLQKKLFNLSKGRFLKTHVLEAKNLKVERELSKSYFAEKDPEPINSYVSVAFAILTDSILKAQSVESEFLFKNIPKYLRPLTFSPDLFKIYMEKDFNNAIKILSSEPMSLWETNPNIRLYILEKLIQYKVWDIAQQFSNSLYYYFTDLKFKQTDHLYTKDSLSKLKLEIDFKLNNNNNNNENNNNNNNNNSLNNSNNNNSENTVTEDDNNEPTYKITENNLNFLKKLLVIDANRNKQKKNKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5R0</accession>
    <name>A0A8J4V5R0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_003742</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000121">
        <property type="protein sequence ID" value="KAF2074957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5R0"/>
    <dbReference type="OrthoDB" id="69177at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF203">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT FE(2+) 2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="6">
        <location>
            <begin position="122"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2074957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="237" mass="27102" checksum="E1995F52E56F296B" modified="2022-05-25" version="1">MEILEFNIDNNSFDKSLIKLSNIDGVQDSFILDNVLSEQECDLIIESVFNGEHHNSLTSQPVLFRGWSENPDDEYKKIGKRVIRTSKSISKILSHRIESFIVKNKSTSNLNGKQINWGYTGLSHRHRFISYEQGQVFPTHMDGSYNKSDNESSFLTCLFFLNSSGSDYSGGVLEFLTEENEQPDQSKPSYEVSVISRVEPKKGRVVIFPHKILHQSSQITNGTKYLIRNDIMFEIIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5U8</accession>
    <name>A0A8J4V5U8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003515</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000111">
        <property type="protein sequence ID" value="KAF2075167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5U8"/>
    <dbReference type="OrthoDB" id="18757at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053369">
        <property type="entry name" value="SrfA-induced_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32256">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32256:SF18">
        <property type="entry name" value="SRFA-INDUCED GENE K PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035277174" description="EGF-like domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="413" mass="44740" checksum="B04EE2B963D2BF8B" modified="2022-05-25" version="1">MKFSKSLLCSLLLLSTCLLSSTLGADQQCSDDYQCGNFPQAACAGKLNLIPGIGLNEQTGKVLIAGKYNSTHQGPFGYPYILSVPIGGGSIQKEYPIKVEIPAGSGYTSVDELFAYLPVKNLMYLRLNKGGSPVIGVYDSNTANFSQVWNIFNTPLEIAFNEQKNQIIYSAYGIYRLNYIPTSKPDYQDTQQVYYQSQLPNGLSNQGEDLYMSTYSGNFYKGLTTCNACPETDLKLLFSDPEGKSITGFTTSPTHMYYSSPNGIFEVPLAGNISMKRALVSDNVVAMVISSNGFIYYTTAAGEVKSVSLAGNHPQVVTLYTSSVGGTCQCAVGFTGDKCQTCQGTILWNDGYPYCYPLVDNKPSTCFYSYQCGEQPYNVCAGSCICASNFTGPDCKTCAGTVEWSNGNAICKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V645</accession>
    <name>A0A8J4V645_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thiol-disulfide oxidoreductase DCC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006246</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000280">
        <property type="protein sequence ID" value="KAF2072434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V645"/>
    <dbReference type="OrthoDB" id="410458at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007263">
        <property type="entry name" value="DCC1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052927">
        <property type="entry name" value="DCC_oxidoreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33639:SF2">
        <property type="entry name" value="DUF393 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33639">
        <property type="entry name" value="THIOL-DISULFIDE OXIDOREDUCTASE DCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04134">
        <property type="entry name" value="DCC1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2072434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="21544" checksum="E6D3DBD8CD12D12F" modified="2022-05-25" version="1">MSEEISLDMDTLSNSTDSNNQMLNNVDGASIVKRNSYKPSNRSIILFDGVCNVCDGFVQFVLPRDINKKFYFQALQTEKGKEIQEYYGIKTDLSTVVLVEEETNSYCTKSTAILRVLSQLCYPYSAFYYLSYIPAFFRDFCYSTFVPYRYLIMGKKDTCMFSPSLKERFIDFKSPLILEELDSKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V767</accession>
    <name>A0A8J4V767_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002161</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000057">
        <property type="protein sequence ID" value="KAF2076547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V767"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="120" mass="12801" checksum="DB397F41DBE4A9CB" modified="2022-05-25" version="1">MSTEVADTTATTKKPAAATKKKPAAKRSAPIQVVAPTKTTSRTITPPTRLIEQEQLAEARRKEKSKKAQETARKAALASTKVKKTTTGAKKSTATKKKAPASKSKTTANKKKTSAPKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7F1</accession>
    <name>A0A8J4V7F1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_004813</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000177">
        <property type="protein sequence ID" value="KAF2073889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10009520at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14364">
        <property type="entry name" value="CUE_ASCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22583">
        <property type="entry name" value="Rcat_RBR_ARI7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041800">
        <property type="entry name" value="ASCC2_CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00546">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CUE" evidence="12">
        <location>
            <begin position="455"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="889"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="893"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="183"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="332"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="428"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="511"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="649"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="745"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1170"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1426"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="11"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="122"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="334"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="387"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="430"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="511"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="578"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="650"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="681"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="714"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="745"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="762"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="783"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1434"/>
            <end position="1444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2073889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1460" mass="164574" checksum="4E4025350D8909C5" modified="2022-05-25" version="1">MGIQNSKISHTISNPTTATITTTSTGSTTGLNNNNVTTAKSRKKVKRKEKDEKKKEKKKANTGGAVIETSSLTLSRNEKDRDQRTGGDGDQDNSESDQHHHFDQNENDTDDKDGCGYSGDCGDNEDIDDDGEEDEDEYDIIDDTMDDNTFLPKITDSSSTINNILPIPFFSISTNSATGTPTSFNGIGSSGSNGVGSNGTLNGNGTTSPLAMSRNKDWFFMENIDQNILNKDNDPHIIFLSAEDIVYVLESQVIQVSDILGLSMGWSTVLLIHYKWNYDQLIQCFVEKGEEYIFTQSGLPPSHCNGARTKDPLDRANFIKLLLSVKDIAKKKSSTSTSSTPTTNGAGGGSSGNAPLSPPVGASASPYHPYRPQPPVGIPLDNGASQSNRRNGTNRNSGNFDDTYYWQPQQQRNISSGANRGDIDDEPMNHNHYNVNNNHRINSNNSNNNSSNTSVYHSLVAQIKEVFPALSNREISNILSQYDNNYERVINAILEGQLSLPKDNNNDNIINNNINNNSNSNNNNNSITNSNISNTSSNISISSMSSMSSNNSDTPDNSNSNNSNNNNRFITQTPATPSPIRNNFSPNNNSSSSSSNNNNNNNHRNPSQYHKRAYSQPGKRLNHLKLYDLDNPPPSPRPVRRLPFITQIQRDQREQQREQQQQQREHAEVSSNSIGMYRNLPLSQSTPFLFSNRSNSPIDHTNTESTSSHHQNPTTTTTTTTTLTTTSKNNLHNLFCSMADKDKASNSINNTNNHDSNQSPNTSTHKPLTYSSDNYSFSSKNNDEEDEEEEDVDEEDEEEEDEEQENYILRNYRKRDTADNNSMENRNLSLKDTQILDIASYELENDIFNYSNNQKKTTTTTTTTTTTTIECKQDNGKESIYGTTDDTESEVLCNVCFCETSQTSCYKLDCDHLYCLSCWDSYLVSKIDEGPSCLYTRCINPQCKQSLNLYHFKYILRNNSEYFQKYLWIFLKSFIDHNPKTKWCTNPESCGKVAFYSGVVDLPLNMNVTCSGCTWRFCFDCGDETHTPCTCKEMGDWKALMSKEGHNVVWLSQNTKKCPKCKIHIEKNEGCMHMVCSNCRHEFCWLCKGPWYEHGDRTGGFYTCNRYDPLKHDTPDLDKTFGTYAHHFQRYSYHHNAKKIASHKLTQLENYKSYYLQQIKKQLQQFIQSQQQQQQQNNGTPTTATPNYNNNNNNNIPCSLSSLSITGELEDIETTSKQLWDRDFLFDCIHLAIECRNILKYTYVYGFYLTDDKERELFEFLQEDLEKSTEHLCEIVFKEIESLEGVNYLSERNRILSSNASYSSITSGSSLSNRIIFTPNSNNSGSGGSGRIGFNNRSHSTPIIMNNHNHHHQHHIDDLISTPTSMASFNSGSGGSTSSTTTTTTRITHENPEHQHIKNYYKITKKFLENLVNGIKNGLIADYGKYSPPPKPVNQKRKGRRGLGKRTNSSIAIFKKFIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V9Q7</accession>
    <name>A0A8J4V9Q7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_002321</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000063">
        <property type="protein sequence ID" value="KAF2076364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V9Q7"/>
    <dbReference type="OrthoDB" id="256333at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08278">
        <property type="entry name" value="benzyl_alcohol_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000003">
        <property type="entry name" value="S-(hydroxymethyl)glutathione dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43350:SF2">
        <property type="entry name" value="GROES-LIKE ZINC-BINDING ALCOHOL DEHYDROGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43350">
        <property type="entry name" value="NAD-DEPENDENT ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="7">
        <location>
            <begin position="10"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2076364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="367" mass="39896" checksum="4D8878AE4E3458D9" modified="2022-05-25" version="1">MKTKAAVLFKNKDLFQIKEIEVDELRSDEILVRIVGVGVCHTDEVIRQNEGLSPFPIILGHEGSGVVEQVGSGVDDFKIGDYVVLSFSFCSKCDTCISGHPNVCETFGDVNFLGKIPRQVSPYQHNGEKLNVFFGQSSFSQYCVVRANDAVKVNVDNEKELSLMGPLGCGIQTGCGTVLNALKPAVGDDILIAGAGGVGIAAVMGAKLAGCRNIIIIDILDHRLELAKSLGATHIINGKRQNLVEEVLKITNGKGVHFALDATGVTPLIVQQLHCIRTLGTMCIIGYARKIEIDLFQDIVLKCIRIVPVSEGNSIPKIFIPKLVELYKQGKLPFDKLVEFYDFNDINQAFNDSASGKVIKPILLMPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VAI0</accession>
    <name>A0A8J4VAI0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit J</fullName>
            <shortName evidence="4">eIF3j</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_001544</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eIF-3 subunit J family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000037">
        <property type="protein sequence ID" value="KAF2077159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VAI0"/>
    <dbReference type="OrthoDB" id="20381at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.60">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03009">
        <property type="entry name" value="eIF3j"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023194">
        <property type="entry name" value="eIF3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013906">
        <property type="entry name" value="eIF3j"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08597">
        <property type="entry name" value="eIF3_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="10"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2077159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="226" mass="25903" checksum="C6135CF80D571515" modified="2022-05-25" version="1">MSNWDDEDFESSKWADENREGADVDEWDAESEEEEANDTPKPVVEKPKPALTKKQALQKALELKEKEERESKNKPTSEFDIYMDEKRKKELQEASDLENSKTLFSGLAVSTNTGASPIDSFKPSTEKEFIQYADMLSDYIKKYDTSIHYLEFFKQILKKTLPDTSSKDLGEISKALNVMLNEKIKNEKNTGKGKKPAKAAVAPKRSLGRMDDEIKTYDDFDEDDFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJ69</accession>
    <name>A0A8J4PJ69_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Dynein light intermediate chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_010460</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in binding dynein to membranous organelles or chromosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer. The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
            <location evidence="1 11">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the dynein light intermediate chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001128">
        <property type="protein sequence ID" value="KAF2068215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJ69"/>
    <dbReference type="OrthoDB" id="27603at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008467">
        <property type="entry name" value="Dynein1_light_intermed_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022780">
        <property type="entry name" value="Dynein_light_int_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12688">
        <property type="entry name" value="DYNEIN LIGHT INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12688:SF0">
        <property type="entry name" value="DYNEIN LIGHT INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05783">
        <property type="entry name" value="DLIC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8 11" id="KW-0243">Dynein</keyword>
    <keyword evidence="5 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="379"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="441"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="385"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="402"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="444"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="456"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="471"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2068215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="485" mass="54306" checksum="99C251F13BB62E87" modified="2022-05-25" version="1">MSGNSLVEEEDIWGQILRESSNKTNYFETKHVVILGDPTSGKSLLLSKFNTISDVESLKSIALSYTFSDIYEDDSSEDPIGRINFWSLEGESTQNDLLKFALNKENIKNSMIIITLDFSQPWSIVESLKKWLGVLEEQIKQIFKDDKSLFRELQNRLLIKWHEYVEPAQNTTAGTSSVNGGNANSNNIPSILANVQKKKKRRVNTSTIEDTSVLPPLGENILINNLGVPILVACCKSDSVVMLEKDFAYKDELFDYIQQYLRRICLQYGAGLIYTSARKEINCDVALEYIENTLFGFELRAKNQLLEKDQIFVPAGWDTLGKITVDFENQKVCKDADEPYENIVKKPSIVKRREMNQVSAIVADEDQEYLIKIKNQLDNLPEDMTPSTPTQLPSSPNPVDPTSPTNPLRTSSSGVPAAGTQSQVAASPSAERAALANFFTSLISKDRTSSRKDLKSSLTSPPSSTPSARQDAQKELDKLKQQQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJG8</accession>
    <name>A0A8J4PJG8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 4">Proteasome assembly chaperone 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_010010</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Forms a heterodimer with PSMG1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the PSMG2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000884">
        <property type="protein sequence ID" value="KAF2068665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJG8"/>
    <dbReference type="OrthoDB" id="10260712at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016562">
        <property type="entry name" value="Proteasome_assmbl_chp_2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12970">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12970:SF1">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010044">
        <property type="entry name" value="UCP010044"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00019186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="269" mass="30360" checksum="17CE3DDD7630083E" modified="2022-05-25" version="1">MSFFYKASNIKDNVSFKDNILIWPALTLGNVGQLSIDLLICTYKFERVGFIVDSNILPIVGNDTYTPKGQGVLSTSIEVYKSKEYQNVTLVQQRSPVIQGHIPIFAKNLMNWSTSQQFKEILFVASTNANKRIDSQLTGNQLRFIKSNTISNETLDKIKLNNIPEMEVQFEQAGEIVQLSNRLTGLGKELYNLCNNQQDTNTTSTQNTPFLCFNLFCSEGDNTMESMAMAHTLAVYLDICKNPTEKIPFVTPPSWALLYGPSFDQSLYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKC6</accession>
    <name>A0A8J4PKC6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000661">
        <property type="protein sequence ID" value="KAF2069445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKC6"/>
    <dbReference type="OrthoDB" id="19216at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="342"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="372"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="467" mass="54296" checksum="6090FE272B6FC61E" modified="2022-05-25" version="1">MIPRIHNQLPLSSLNTINYFSTTNDCQQDTNTTTTTNDNSYIAPSTNDEFEIEFPSLSEIYNSGLYSRFNNTCSINGGNSIGSRVQNERIVREKQRRQQQQQQKEMEQQQDSIRKSNISPELEQEENEILNAYNNEVLNKHGWKKRDLLYYCFIQKNHENDRIANFIHSVSLYYKTSRECLLDIGSGIGRMFSYWISLKWELIEAHEPDFDYYSFSNDYAKNLSDKIFVYQSGFLDLNVVAKYDMVVSIHGPLQYLTDVRDRFKALQNIYNSLKPGGVVLLDVSNFLSGLTNLCTQMNQQKMMVNGVTVKRISNIQVNTLLGLWQTDDVFFSVLENRKYSTLDDDFNDNDNDDQEDDNDINNQYNDNDEYDNFQQQQQQEQHQQQQSDKVDMDNSSFDIEKNDSGIIAEQHTFSILTLSELQLLLMSTGFINIKHASKWDFSSFISHDQELDSNGHRIIITGQKPFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL88</accession>
    <name>A0A8J4PL88_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010479</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001140">
        <property type="protein sequence ID" value="KAF2068196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL88"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="761"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="761"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="853" mass="98290" checksum="FB72371894F2B973" modified="2022-05-25" version="1" fragment="single">MDLDDNNSSSSDIPLDIESLRIIISSTDTYSPKLATIIPSTIIELELQFIIDKRPNAEITSDQQIEIICLKLKGFIIPSTITVLSLKVSYYGSEKTGYEDSTLQERYEILKTIISNNKNLKRKYDDIDDVNQYFIPPNVTKLILPSIDDGFIQLVPPTVTSLRLRKQVECVKLDLQLLTSVIHLEVASKDSVLRCISLPSSIQTITTDNTMRFECDLNENIRVNYIYFQQNFAYYGVYEDDDPEEIEKGRLIELRNERSASLVDSIPTIAPNLKIVSFYDNWPIPSKFMEGIEYLIVHYPINPYFPQQAYPSRSLTFKFFKIHMNDRQVQLLKLPITTTHLEIPLSHGWTTKFNFIPRSISHLKIMKPKRLDYSLENEEYEQIFIPSTVRFIRLFDNRYKSKIRIIDEQEYYLKPRNGLKKLSLTIQDRDFIFTENSLPSGLEELCIDGDGCLEEYITLKNTIPDSITKLKIPQQTKFDDSPPSLTSLDCYGHHLRRFNSCHIQDLFLRNASNWSNVAENNWPIKTLGFSSQDNHVQEIPNTVENLVLGNIDVKLTKLHNIKNLTLVGKARFDSMPDTIEILDIGDSTHKLPNILPKSLKELTLSYHDNIDRIPKKPTKPFEMKIIENYISSTSNIYSIDSYFKIWRNITLKNSIIDYLINPNDQIICELREFSEAKEPNRYFEADINPHTLIVEPIIPIDKIIGLNILNHNVKKSLHLLPSNIDRLIVHLDSKSDDLDFTNLPKSIKYIKIVENDIDNDIDNDTDNDIDNDTDNDTGNDIDNDTGNDIDIDIDNDIDNDDDKDQSPPLQTIILNAFIKCLFVSSQIYPFKILIRQGPDDDQPTSSLLESNNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLQ5</accession>
    <name>A0A8J4PLQ5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008404</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000515">
        <property type="protein sequence ID" value="KAF2070272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLQ5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="109" mass="12663" checksum="BD742F0E3C388C7B" modified="2022-05-25" version="1" fragment="single">MSSDFSMDDFDDFDNNIPINTNISTKDLFEGSDARLPLANSINSTIQSLYLPNSFMDQPQMHQMHQQQQQQQQQQQYYQMPLNQSHGDYTNLGRSNNLTPRTNLQQSMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMI9</accession>
    <name>A0A8J4PMI9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glyoxalase I</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_008003</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(R)-S-lactoylglutathione = methylglyoxal + glutathione</text>
            <dbReference type="Rhea" id="RHEA:19069"/>
            <dbReference type="ChEBI" id="CHEBI:17158"/>
            <dbReference type="ChEBI" id="CHEBI:57474"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="EC" id="4.4.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit. In the homodimer, two zinc ions are bound between subunits.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000460">
        <property type="protein sequence ID" value="KAF2070677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMI9"/>
    <dbReference type="OrthoDB" id="16820at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004462">
        <property type="term" value="F:lactoylglutathione lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019243">
        <property type="term" value="P:methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16358">
        <property type="entry name" value="GlxI_Ni"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004361">
        <property type="entry name" value="Glyoxalase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00068">
        <property type="entry name" value="glyox_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46036">
        <property type="entry name" value="LACTOYLGLUTATHIONE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46036:SF5">
        <property type="entry name" value="LACTOYLGLUTATHIONE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="VOC" evidence="6">
        <location>
            <begin position="141"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00030537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00048273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR604361-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR604361-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2070677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="274" mass="30966" checksum="6B536CDB2B66B53A" modified="2022-05-25" version="1">MRRTNIWVYRNNNKNTCFEVPYEIFNIGALRLWMQKRRVAGTEELFGYHLVQKDLQECSIQTIESNFIQMRNTIALPRNSDGSLWIHIENFQEVKKSKKGVGKKERKKKKKPCLPCTLPLFNDECSEKQRPPPPLTRRSPQIKAEPPVVGNLDRSIDFYTKVLGMKLLRKSENTEYKYTLAFVGYTDESSGAVIELTYNWGVESYDLGSAFGHIAIGVDNVASTCDSVRNAGGKVTREAGPVKGGKTIIGFVEDPDGYKIELIQDDQASQGLGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNJ8</accession>
    <name>A0A8J4PNJ8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008244</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000492">
        <property type="protein sequence ID" value="KAF2070433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNJ8"/>
    <dbReference type="OrthoDB" id="26095at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053331">
        <property type="entry name" value="EGF-like_comC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24032:SF24">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24032">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="918"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="661"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="665"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="684"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="973" mass="109041" checksum="2F439E56CB30C844" modified="2022-05-25" version="1">MNLLNTTIINILFILSFVNLIFCNPLLPRPEYDNLLSLRTHFKLENDYPEPIDFCSRSNIFKCNPNNQSILSINLNGDGSTVIPSFYNFTTLEYLKMKNVIDANHFLVNYNQFFSSQISLECRNCNISRFANLNKKYAILILIDNPISGEIDLGNTLYSFFEIQNYNPLYLFYSHKYISSFCPLQVNIQKLTLQITANIDVDFNFNSTIDTSFPNLAMRPINLKLIGNDLISNNKYNFPQIPSLNYLELENINFKPDFVPSLAIQNIMATNSSLSTSNGELCIDLTQLDYIALINVGVTILPDITTFKRARILSLQNNDMIGTFPLNLNNTVVYSFDNNKLTGQLDQSVCTGYVSFANNKLTGPLPSCLSCYLKNSAIRERVIGNQFSNYNNDQVYPPCDDVQINWFSNITGQGFDIKGVNLPFMKSDITLNIPKVAISTSNGTYIAFNFDGITSTQVLETYKEIIFTIHTTNRDFIFKYPLVQQYPSLLVISDAQPLLNNGGYYFFFIFANLQSDTLVKVTMDNYQCILERRADATIRCAIQENFPEKIYTFTFTFNLPYTISQQKRFVRKFPIVTATSLVPKSGGLVTIYGTFGNNITNSNIPNVTVSIGQLACAVQSLVDYLIECKLPPSGASGPQNISVTIGGVRGFFPNLFLYSEDAAQCPKSCSGNGVCLGKIGMCLCYYGWGGADCSNRQLMGSISHNGNTTEISQDQVRFGLTIVGIQELSFDGKLLKERIFDRWNQTTNNETIFSYTHLYEDSIMGYQIEEITQQKSFEFAGYNMSLDAGTFKLTANVSNWVFISSLSSLQLLINTSLVSSEDDCTQTEINSFGESTLTHITIEKDGKIFYGKFIDRMLSDGKPTYSSTKIVNQTSNSILLGISLPHCTQCILDPDFSILISNKEQNGCKSTTTSNRDWVVPTAVVVSIVGAAIIGVVISVLLSRFYYIKISRKGVIFIKKEKKKSGADGIQMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNR3</accession>
    <name>A0A8J4PNR3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007351</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000387">
        <property type="protein sequence ID" value="KAF2071320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNR3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="67" mass="6767" checksum="CEB2AE68A99A73CD" modified="2022-05-25" version="1">MTLIKSITSLGSVQSGAISKSTISSSTGSLSFKSNGIGANQNSSLADIDIGAWLLNILGISIKAKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP79</accession>
    <name>A0A8J4PP79_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_007824</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000439">
        <property type="protein sequence ID" value="KAF2070853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP79"/>
    <dbReference type="OrthoDB" id="21428at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034967">
        <property type="term" value="C:Set3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46462">
        <property type="entry name" value="UPSET, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46462:SF3">
        <property type="entry name" value="UPSET, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="3">
        <location>
            <begin position="294"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="222"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="514"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="583"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="685"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="56"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="222"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="234"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="521"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="596"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="685"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="699"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="737"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="758"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="772"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="807"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="838"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="872"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="973"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="995"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1023"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1048" mass="118391" checksum="4F3A70DAA8207551" modified="2022-05-25" version="1" fragment="single">TSPAISSASSSPLTTSKKSAPNTPNIPEESLSSVKEDGGDSLASSTDQDIVMTDSNENKNDMENSEKDTPLASPRILLKDMDMSDVTITYLEDTQSIEKLLESFNSIKYPKESKYFSDALDIFSFITSIVLREQLIDDYCKRKQVNESSTTTATKTCINNLSVDDNILFQKYSLLYLENSSHPLKKALSLLLDIKEETVHTFMAEYAKELVNQMENDFSKKVTQNSSLSTEMDTSNDNDDDDDDYDQEKRKWKLIKDSIYEKEFKVPSDFMEFQSNNLFNYPLVDLSTEKAIVSKVSKASTTKSTTNELKMTQSYKCLLSKYNLDINTFIGEITGIFGNDLSMLKNEWADSNSWLLNNSAPNQYVFCFNSTYCYSRIPFSTNASNDVNSFCSKETDFSIDARDQGSIGRFVRRSCCPNAKIQFSLEPPTTTQESNNSQLRAGIVCKDEIPQNTEIAIAFDYPFKSLKNRIYCPCKTSTCYVNSWFNERASLGIKLLELLGETVDPTLKQNESLTVIKEETSSNLDETNSNNSNSNETDSTPNPPKKKRKGKSSLLELDPIFLQGSETSAREERKLQSIIQNFEQQQERNKEKQRKTSPTNTSPKKKSPTSISPNTLSPSSSSANSPSLQPTTTTTTTTTTTTTSTSNLSFENNGVKESLVSEEENGTKLSKFAKKAWLAEFKQKEKELPSIKEDHESSPSKATTASPSTSKETTTDDQQSTNESEHQEEEGSLPNEPLSSNIQSKSRQENHSASPKEQQQQQQQPQSPYYHGSHHQNSYHHHHHGHYEGQKSSYPPHDREIRHGHYTPTQSPNQSPSIKGFGGGGGSFRDPIDQSYRLSRERNDNRDRDERDWDRAGGGSRESRDWPPPQSYDRDFDSRERRYPPHPRYKEDWIDHRKTGWEGSRGYDKGRGPPPPPHPYERSNSAGSNQGGEKSGWDRRPLSNSGNYPRNGFSPPLSYSSHDADYDSYENGSPKSSSSKQTHSPQLDSPSGFKSSYNPQSRNNYNNSNNNNSNNSKYQTNSPTLSQDDYPTQDQWNNRKPYFNKRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPD1</accession>
    <name>A0A8J4PPD1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000472">
        <property type="protein sequence ID" value="KAF2070580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPD1"/>
    <dbReference type="OrthoDB" id="22524at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF173">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1199" mass="138518" checksum="33D096B596754532" modified="2022-05-25" version="1">MPKTTDIDLDHLDLRALIDSKQDLTIYGNKGVAVKILSLIINKSNNDVNHKSINKSVLDRVAQWSMEEQYSEYLEYYVVVYDVQVRDFLLCLQRFIKQESEKALYTGISGSFKMFSLMFSFNINYQSESDDEIVEIIRLLKSKILSIRIQIHETTGDFKNKTIESVYTVDFLVNRLEQEFESTCLNKKYLFKKISNNSVGENYLYQESVHYRLFNPDTDEDDLPLDTEILDWDIQILPLEGSIPTSVKWLKLLEEIPEINESVIPDNILCLSLKYYNYSLSQIRLPPKLKFFSIPSFTRFLYRNLDVPETITHLEISIDYAEDIACHWLTIRGTLPRHVTHLKIASNLSIHSITKEKFVIPSSVQKLDIDQNDAVYFQVIQESEYYKYPMTRDYVACISSLKSMSFAEGYLDTEQPLYSNLFSSNLESLDISYYAQLFDANVLPQSLTSYTCRHFQQIPDHTRLNYLEYNIDDFKLIFKVNDRSITKDRIPVSFDPKTGVFNPKPTLPIEEFDNGSKFSVRISSGANIRKITDVSNAILPNSVQVYESSYSLPLNYPESLHTIILKREIEFDIYSIPPTVKVLHCYLTPAMMSSISKPIDLLLKDKYKSVNINKVIVVNIDSFFQIWRNKYLQNKIQRLYQARQIDFKVTRNRESGLVFNFQNDCPLPNHPLKFKTQELCTIPHGVTFCSFSNPFHEEKSFLLESLPGSVKKLKITYYNSEAIKLPPTIKHLVIKSNSLHILDYIPPSVEILELNINDIGVHLFENRSLTAKQVIIKDKLFASDNVGPEVIKYLNERNLFGKNQYIQLYNEYEQISPSTTVLVWRLDEIIPVGLIPFGVKKIIFGDFFNQTILENSIPLSVTEIYFGWKFDQSLLHVYLPVLLKTLTLQHYNHPIHPNTYPPNLTYLNIKDEQTNLVHLPKTIKHLKIMKTKLHQDYDNNSTLFKVKSIYSNPSPVDIDLIERLNQEIDIFKTLSDDSQKKTNLELHFTLTRDLSILPGSLNHLNIKSIRIPNEYGNTFNQALLEGSIPSTVTTFELRNTEYNPKVHSIPSSITKLALQDIVPIPLHNGIKVLECQTIADIVLPNTIEKFSLYNSPYCNLKILPSSVTKLCLGFADESMFDTIPPTVTKLTLDCDVHHLSNLPRSITKLKCREISTIQDLPTTVKCLHLHSSHFIGRLPSTLEYLKIKNIDRHLLEINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPH2</accession>
    <name>A0A8J4PPH2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SAP DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_007971</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000455">
        <property type="protein sequence ID" value="KAF2070717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPH2"/>
    <dbReference type="OrthoDB" id="639027at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15241:SF304">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15241">
        <property type="entry name" value="TRANSFORMER-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="SAP" evidence="4">
        <location>
            <begin position="37"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="352"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="440"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="517"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="816"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="932"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="125"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="157"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="190"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="562"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="580"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="632"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="665"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="816"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="849"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="936"/>
            <end position="960"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2070717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="960" mass="107254" checksum="F9E1626CEBD02428" modified="2022-05-25" version="1">MGSDTNTTSTATTTTAETANGSTTTATATLEKIPNDLKSLTVSQLKTILTQRNQPITGTKSILIQRLEKYRELESINGGGEEEEEEEDEEKDGKVEKKDDKEEKEEKDEKEQQQQQEEEEEKDNNSSSTSNNNSNENSDNIQADDQEDDNSNNNKNEEEEDDDNQQGDDDNEVSEYANQEYSDDEDSKKTNNTAATPSNTTTTTTTTTTTTSTNKNNNNNSNNEDEANIVAQAISEAEKAVSEKLTAEKKETIDKSKVSQAIAEMEESFKKTPLVKVTGLNDTVNEAELKEHLTKHGGPNITIGAIVVEGAKKDIAYVQVDGGLNAIAKDKVEAEKLKNCTLSLTEAPIAESLLFVGNLSNEVTKDELLKMFEKHGTIDRVILIKNKRTGDSKGYGFIDFRTKQQANTAKSSLGSLVLNRRTLRVDWADNCSTLESMHSKTIFVDRLPRSFTDTFVLKKLFSPFGKIKDCNVVPNAYGQPRGFAFIDFETVEDAEKAQRMMNEKDLQGFKIRVNFGNPSKPGFSLVKPQRNENSEGGHGGGYNNRNNRYNNDRHNNNNNNNRNDRGHHGNERGGRRGGHRNSGGGNSERGGGHHPFAAGGYNNNNHMNHQGGPPFNGAPFEHQRGRGNAGGPHPPFYPPPIFHPLTPAGAPMQVHPTGPRPNLPNNHRNNNHHQQQQQHPHQQHQQQQQQANQSNIKAQTQLAQAQAQIQMMEQKIKSQQELLARSQATQAAQAAQAQREKLQREAVIKAQMQEKQMIKAQQEALQKQQQQLLQQQQQAQLQQQQQQAAAAAAAAAAQQQQLHSQTYAANQSNQYQGYYQQQSKPAASTPPTASTPNTTYPTVPGATWPQTQQQQPPFQQQQQQQQQYSAYYTPEQAAWAAAYYSNPYNQDMSSYYASYPQTPVGDQTQQVVGQKRPYDNTHQNTYQGYHQNTHQNTHHNTHHNTTAHHNTHHDNKRNRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQM0</accession>
    <name>A0A8J4PQM0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TRAF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_006683</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probable adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000319">
        <property type="protein sequence ID" value="KAF2071994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQM0"/>
    <dbReference type="OrthoDB" id="10062218at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001293">
        <property type="entry name" value="Znf_TRAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131">
        <property type="entry name" value="TNF RECEPTOR ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131:SF94">
        <property type="entry name" value="TNF RECEPTOR-ASSOCIATED FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02176">
        <property type="entry name" value="zf-TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50145">
        <property type="entry name" value="ZF_TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TRAF-type" evidence="9">
        <location>
            <begin position="167"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TRAF-type" evidence="8">
        <location>
            <begin position="167"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2071994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="310" mass="36674" checksum="B9E879A6FEF5AF44" modified="2022-05-25" version="1">MEEKDKTLSYSNPRFIEEKIEYSLEDVLVNKKDIYNCSICLDIKPVLYQCYNGHMNCLQCWENHLKTNKTCPVCRLDITLQTLAQNLALKFFLSQEMMKCPYSNLCESMNYGTFKDKHNNECLYRSIKCQCSVIMKFNEFENHQKQCPKSKFICPHCFIVFNRDSNHLEICDEMDISCHQCKTKIKRKELIDHVRHECPKTKITCPLKYLGCRIAFKREDKKSHFTSIQHLNVCMDISDQPVFMTTLERGTFETKFQFLVVDSSFSYKIEDEDLVTKINNLKYQSFAKDFYFTISIYDKNNNLLSSGSKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQV7</accession>
    <name>A0A8J4PQV7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dephospho-CoA kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_006437</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CoaE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000297">
        <property type="protein sequence ID" value="KAF2072237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQV7"/>
    <dbReference type="OrthoDB" id="247245at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004140">
        <property type="term" value="F:dephospho-CoA kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02022">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000991">
        <property type="entry name" value="Dephospho-CoA kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00376">
        <property type="entry name" value="Dephospho_CoA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001977">
        <property type="entry name" value="Depp_CoAkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00152">
        <property type="entry name" value="dephospho-CoA kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695:SF46">
        <property type="entry name" value="BIFUNCTIONAL COENZYME A SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695">
        <property type="entry name" value="DEPHOSPHO-COA KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01121">
        <property type="entry name" value="CoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51219">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2072237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="201" mass="22696" checksum="57E899205EA221AA" modified="2022-05-25" version="1">MIRIGLTGGVASGKSTILKYLQALNIHCIDADKVGHMVYEKDTPSYHKLLSIFGNNILAEDQTIDRKKLGPIVFSDPNEMKKLTDVTWPAMKSLILNEFENHKQDKIVVLEAAVLIEAGFTDVVDKVWVTQVPREVAISRLSERNNYNAEFAEKILSRQLTNEQRATYADVIFDTNGDYQVTKDKVINEVNKLLNQSSDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSZ5</accession>
    <name>A0A8J4PSZ5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_005412</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000215">
        <property type="protein sequence ID" value="KAF2073283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSZ5"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827:SF85">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827">
        <property type="entry name" value="RETICULOCALBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="7"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="43"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2073283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="164" mass="18665" checksum="6639E5B2FB397AC1" modified="2022-05-25" version="1">MGSINKQVGEEVKNFLSKFDKNKDHVVSYDEVVNYWKTQDCKDPKGSADLIFTALDKNHDGSISLSEFIKNTASVKRLLVKNRVVEDNLVNFLLKFNKNSDDVVTKSELEAEFERSGHKNPKNAVNDILYAMDADNNDSITMNEITESFKLKSAVYKKRQAPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT62</accession>
    <name>A0A8J4PT62_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005648</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000231">
        <property type="protein sequence ID" value="KAF2073032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT62"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="190" mass="21704" checksum="6D36BA92BAE4B078" modified="2022-05-25" version="1">MEEMKTTLDFHNGHGKEIKVSFETISSKSIVESVTEEEVVKFVEKAHSKRQRETNLTRSWSVEYDEDNQGNDEKESGDDYSDDDNDNSSSDESSSGSGYNQIFMLRDYKKKPFDNLSSTSFLTARGRPIQVEKDKLNHAFETVSKEKEITEEDLSRYFQDLELLNQPLYKEDLKILTNSSSSGGSSTKQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT73</accession>
    <name>A0A8J4PT73_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_004551</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000163">
        <property type="protein sequence ID" value="KAF2074148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT73"/>
    <dbReference type="OrthoDB" id="5970722at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14704">
        <property type="entry name" value="bZIP_HY5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051882">
        <property type="entry name" value="ATF_bZIP_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46164">
        <property type="entry name" value="ATF6, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46164:SF3">
        <property type="entry name" value="ATF6, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="7">
        <location>
            <begin position="41"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="147"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="200"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="258"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="148"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="164"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="200"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="259"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="274"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="309"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2074148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="382" mass="42517" checksum="85B3160095193913" modified="2022-05-25" version="1">MYSPTQIEMSSEPDMPVSPQSQAFKINKMKLDIKSSDDDRAKKRQVRLLKNRQSAALSRNRKKEYVSNLESKAHELKMSSQELHMAFTQLSANQYETRVRLELLEKQLGTIRLENENLAMKIDHVKSKIHTKQNKIDLKSSISFEYHSNNNSNNNNINNNNNNELSSGTSSPIQSESIIGDKVKREREILIGINKYNNSKSNSNINLNSNNNQSKQQSCNNSQTVDKEEVSYHSPQYSFNSLSISNPNVNSSPVYLTRARSSSYHPQSNSFLQTSSTNTTPNTNDESISTPDSSPLLPSLHHITKNLHPTISPATSSPSSSSPLASLSPLMINNNNISGSPHRSNMIGLSKLGKSNLSSSPQLPLIHLQQISSTPSSPLSHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTC3</accession>
    <name>A0A8J4PTC3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005603</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000228">
        <property type="protein sequence ID" value="KAF2073077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTC3"/>
    <dbReference type="OrthoDB" id="15922at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="274" mass="32052" checksum="AB4431F2227B2F3C" modified="2022-05-25" version="1">MVELYDSFGIKIYVDHNRTTSKVIDLQKKLFKIFLSRKVPHSLISIFDIAYHFDIQKRIKAHSQKIPRYPYIEINGKLWGEGDMVESRIEEIDVLIKELVNRKVESTANEKSLVDAFLSDTEPTDIESKPNLEIGYIDSSLNLIEWLSYGIVSNIWNPWRLPSEPVIPQDSADIEFEVVRTNWYGRHQYRTYRFTPSEIYRIHNNTVKATLFYKDIQLLTKVDSKNIIIKILNCEDQYIQAVEEDISKIIEVVTSRAYLVPFGENEWKSSVALN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTT5</accession>
    <name>A0A8J4PTT5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosome subunit biogenesis protein NIP7 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_006259</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for proper 27S pre-rRNA processing and 60S ribosome subunit assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with pre-ribosome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the NIP7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000281">
        <property type="protein sequence ID" value="KAF2072424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTT5"/>
    <dbReference type="OrthoDB" id="27490at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042255">
        <property type="term" value="P:ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21146">
        <property type="entry name" value="Nip7_N_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21151">
        <property type="entry name" value="PUA_Nip7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.10:FF:000002">
        <property type="entry name" value="60S ribosome subunit biogenesis protein NIP7 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.220:FF:000001">
        <property type="entry name" value="60S ribosome subunit biogenesis protein NIP7 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040598">
        <property type="entry name" value="NIP7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055359">
        <property type="entry name" value="Nip7_N_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016686">
        <property type="entry name" value="Ribosomal_synth_fac_NIP7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005155">
        <property type="entry name" value="UPF0113_PUA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23415">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT/60S RIBOSOME SUBUNIT BIOGENESIS PROTEIN NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17833">
        <property type="entry name" value="pre-PUA_NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03657">
        <property type="entry name" value="UPF0113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017190">
        <property type="entry name" value="Rbsml_synth_fac_NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88802">
        <property type="entry name" value="Pre-PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PUA" evidence="7">
        <location>
            <begin position="95"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR017190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2072424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="180" mass="20762" checksum="FD08793BDF4C414F" modified="2022-05-25" version="1">MRPLTEEESKVFFEKLVKFIGKNVTLLIQRKDEPYCFRIQKNKVYYVSEEIMKRSQNIPRDSLASLGTCFGRFTKSGKFKLHITALDYLAQYAKYKVWVKPQSEMSWMYGNNLLKAGLGRITEDTPANQGVVLFTMTDIPIGFGVTAKSTHECRKLDPPALVVYHLCDVGEYLRDEETLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTY1</accession>
    <name>A0A8J4PTY1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribonuclease H2 subunit B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Ribonuclease HI subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_005359</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000211">
        <property type="protein sequence ID" value="KAF2073341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTY1"/>
    <dbReference type="OrthoDB" id="29098at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032299">
        <property type="term" value="C:ribonuclease H2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040456">
        <property type="entry name" value="RNase_H2_suB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019024">
        <property type="entry name" value="RNase_H2_suB_wHTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041195">
        <property type="entry name" value="Rnh202_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13383">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13383:SF11">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09468">
        <property type="entry name" value="RNase_H2-Ydr279"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17745">
        <property type="entry name" value="Ydr279_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rnh202 triple barrel" evidence="8">
        <location>
            <begin position="77"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Ribonuclease H2 subunit B wHTH" evidence="7">
        <location>
            <begin position="139"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="288"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2073341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="333" mass="38092" checksum="DDEB3FB69EB87C94" modified="2022-05-25" version="1">MSKKQQIDEEDEDFSFSEEEEDNSTKKRKRGSIGDSSSASSSTRPHPPIPTLPYTDRVFMLYQPPTTTPTTDTEGSNILNYNIISLPHPKTNTLCRYITLNKQLLELSKFHNKPSSWFIDNSVRYDGSFYVTTPIDPLYLIIPFLEKMAKQNPKTKEIMYCELSTMINDPIYSHLTKAIEYSHKELDLICDFTDLLGTKLYRLNDKKVLIWLRCKVKLLLSYLKESGTNIFKTAANNSSNSNKQDVSDDIYKTMAIGFIEEYVSDKYILSLKQSFGLEKDKPTSSMKNLIYTEKEEPEPIASKPAKPSAKKSKKKPAAAQANKISHFFTIKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUC5</accession>
    <name>A0A8J4PUC5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Glutamate--cysteine ligase</fullName>
            <ecNumber evidence="3 11">6.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Gamma-ECS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 11">Gamma-glutamylcysteine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_004911</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-cysteine + L-glutamate + ATP = gamma-L-glutamyl-L-cysteine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13285"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glutamate--cysteine ligase type 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.2" evidence="3 11"/>
    <dbReference type="EMBL" id="AJWJ01000183">
        <property type="protein sequence ID" value="KAF2073777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUC5"/>
    <dbReference type="OrthoDB" id="7939818at2759"/>
    <dbReference type="UniPathway" id="UPA00142">
        <property type="reaction ID" value="UER00209"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.50:FF:000009">
        <property type="entry name" value="Glutamate--cysteine ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.50:FF:000002">
        <property type="entry name" value="Glutamate--cysteine ligase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004308">
        <property type="entry name" value="GCS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11164">
        <property type="entry name" value="GLUTAMATE CYSTEINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11164:SF0">
        <property type="entry name" value="GLUTAMATE--CYSTEINE LIGASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03074">
        <property type="entry name" value="GCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="5 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2073777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="613" mass="70525" checksum="65C960E5F62A7357" modified="2022-05-25" version="1">MGFIAEGNTLVWEDAEQYAQYVKDHGIIQFLNIYNANKDRFADQFKWGDEVEYILISKDQDGKSFKLKLRAHEILDKLFQEEKMNPDNVDYLWRPEYGRFMIEGTPGKPYIGMGRQLLAVQTSLQGRRASVERYLKQDESIVTLASYPLLGVKQTFTEPSGQVRGVVAESQFLPDTVINPHFRFSTLTANIRKRRGGNVSINIPMFKDTNTPAYLDSTTYISSPSTSLSSTKPYNIYMDAMGFGMGCCCLQTTFQLSNIEEARTVYDHMAVFAPIMLSLTASSSIFKGYLSDIDARWTVISQSVDDRTPEELGQRELKHNKFVINKSRYDSIDSFLSRSSSFRSEYNDLDLVYDKDIYNKLIEGGVDDLLAKHYAHLFIRDPLVIYSDKITIDDTKHSDHFENIQSTNWQTVRFKPPSPGVDIGWRVEFRPMEVQLTDFQNAAFIVFSALMVKAVSDLKLNFYVPISKVDENMKTAHNRDSCRVDKFYFRKNIHNNSPNGSIESEYELMTINEIFNGKSDFTGLISIVKRYVQSLNFDFETNQTISKYIDFVSKRASGEIKSISTWTREFVRNHPDYNHDSIVTEKIQTDYLARCLDITNNIIHDPEIQGNFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUD1</accession>
    <name>A0A8J4PUD1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004886</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000182">
        <property type="protein sequence ID" value="KAF2073792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUD1"/>
    <dbReference type="OrthoDB" id="15150at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09083">
        <property type="entry name" value="EEP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF36">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="290" mass="33119" checksum="43A95B70C09E0D3D" modified="2022-05-25" version="1">MIRISTYNIRYDAEKDQHNSWKNRKHLTIGNIVFLGASVIGLQECLHNQIEEIKSELKNKYGLNYSYVGNGRDDGKTHGEYSPILYNNDVVELIHSHMFWVSEKAYQPASRSWDAHCPRVCTWAFLRHKATGKAFYFLNTHLDHMSELARSEGTLMIKSFISTLDHGIPLCLVGDFNAHTDSTPVKNVLDTKQSSSLAHKYLKYLEIPLKDTFSFQTLYNAKSITQIKIGPDATFTGFDLAWKEDIDHVFVNSKFKVQSFVVFNNHPQNVSTKTTIASDHLPVVSDLIFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUF2</accession>
    <name>A0A8J4PUF2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004565</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000164">
        <property type="protein sequence ID" value="KAF2074123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUF2"/>
    <dbReference type="OrthoDB" id="10644790at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="229" mass="25759" checksum="E134759F692FAB56" modified="2022-05-25" version="1">MKFKFLSKKEKQDDKSEQKNQVVPDLNLNIMESPKRSKNHVISPRSISSPVLLESNNKNYNSSTSSSSSSSSINSIEKDKKLNTSNKSNKSTTSSTSTTSSSCSTEPRSKSAIDLKTYFQQNPEFYQNIPTSPKYDYSWNDNNNNHNNNENEINKRLFYSMSRDYFPTTTISKSSSSIQLTFEGGNSNSLCGVIVGCLDTYNPKKYPYNRSNSSYQSATNNTTDIQQSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUL9</accession>
    <name>A0A8J4PUL9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF3825 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003531</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000111">
        <property type="protein sequence ID" value="KAF2075183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUL9"/>
    <dbReference type="OrthoDB" id="20861at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024437">
        <property type="entry name" value="DUF3825"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12873">
        <property type="entry name" value="DUF3825"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3825" evidence="2">
        <location>
            <begin position="112"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="394"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="463"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="512"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="661"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="743"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="394"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="464"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="519"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="690"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="743"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="764"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="784"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="796"/>
            <end position="812"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="812" mass="91903" checksum="76DADCD8588C7279" modified="2022-05-25" version="1">MSNHPTLNKTNRSLSSNSLLNIKNDLNSNSSNNNNNKILNNLIGNGPQSFHLPLNQMKKTISPISSPPATPHSPQPPSGEWRNWNSEALIQCQRFHNLVYYRAGNLKFLSESIAEAELWGKELKVLKIYIDNTFKRCVEENKLLAFLPFDSQTPRLFIFNTGLISRNYEEIYCIIQNMKTATVKERELELGGRKCEERQWMLKEFVQTSLFNDALVFSKYGEGIPSIPKSILPCRPYYFNEGIDRSMYDPRLDIDTKIDINEIIKKSSESKMDRLPLEYSQTSSDELLSRFQHGIKNTIIKLKSNPRCAVPQFHRDSDGQKKIDLLAPLCLGKKRDFDCSLVIRFSPEEQCYKIRGILSKEDSYINARIIQKIDQKWLDYHELHDTVGNISNNTNAINTSGGSSAPSSAPQTPTNAPAMPFGVPTISIPPLNGTTILQSPRYTSKQQQQYPIIPSNAMIGGGVNNNLNNLNNSQQSSSSSNNSPTSSSPNSSTPSTPSTITSAAAVALTIHPPPRAKSPESNLNNNGTTVFLSASDSGLPYGKTKSLYTLNELKSPRLNIIPNAPMIGLNGSNLDLDIVDNNSNNLEYHNSNNQRNQQQPQKVSNYHQFLNSNDIHQSQYNIYNNSNNINNNNNNHHMFVKTNSKSNLFNNLSLHDFNNATSQQQQQPQQLISPRKMIQPSPSPSPRYHNNSSSNNQNNNNNNMNNINNMNLLYQQQLQLQLQFSQIQQQQQQQHYFQQQTQQQQQQQQPQQQAQQHFSHYQNPAFQNNGGQTPRGSSSFYHHNQQQNNINNNNQPKTIYSLSGEQLPQQQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUN1</accession>
    <name>A0A8J4PUN1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Derlin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_004421</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May be involved in the degradation of misfolded endoplasmic reticulum (ER) luminal proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the derlin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000155">
        <property type="protein sequence ID" value="KAF2074288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUN1"/>
    <dbReference type="OrthoDB" id="19102at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007599">
        <property type="entry name" value="DER1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11009">
        <property type="entry name" value="DER1-LIKE PROTEIN, DERLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04511">
        <property type="entry name" value="DER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="217"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="217"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="239"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2074288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="248" mass="28481" checksum="11E8528C6F7DEC02" modified="2022-05-25" version="1">MDREIRNWWSTVPYITKWLFASMAIVTVASGMNIFKPYYLILDWPSTFKQLQIWRPFTATAHLGPLNFSFLIHLVFLYRYSCSLEIGSYAGRTADYLYACIICAVLNIVSAFFMGYRITGSMLIMSIIYIWSRYNADTIVSFFFGIKFKGVYLPWAMMAISFLSGGGHFPFDEFLGCISAHVYYYVNDVYPRVHGTTSYLKTPSFLKNVLPAEIQEQQRAREAEERRAQRPGTRAQGGYNWGTGHALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVF0</accession>
    <name>A0A8J4PVF0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004326</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000151">
        <property type="protein sequence ID" value="KAF2074350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVF0"/>
    <dbReference type="OrthoDB" id="843225at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="110" mass="12414" checksum="C35EBD8E70E65CE3" modified="2022-05-25" version="1">MKYLVCVDGSTQGRYAANKAMSMLTEDDTVYFISVYPPPPKPDEHGSPTASTLTKMVIELFSGVPDLVDSSSDDPNKMIRRSNKKKTEAINEFGFFKTFLDKKKLMFTIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWA5</accession>
    <name>A0A8J4PWA5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA/rRNA methyltransferase SpoU type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_004394</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000154">
        <property type="protein sequence ID" value="KAF2074300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWA5"/>
    <dbReference type="OrthoDB" id="241340at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002938">
        <property type="term" value="P:tRNA guanine ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18092">
        <property type="entry name" value="SpoU-like_TrmH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033671">
        <property type="entry name" value="TrmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43453">
        <property type="entry name" value="RRNA METHYLASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43453:SF3">
        <property type="entry name" value="TRNA_RRNA METHYLTRANSFERASE SPOU TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="tRNA/rRNA methyltransferase SpoU type" evidence="4">
        <location>
            <begin position="50"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA/rRNA methyltransferase SpoU type" evidence="4">
        <location>
            <begin position="240"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2074300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="348" mass="39614" checksum="B6F21D376CC0EE7D" modified="2022-05-25" version="1">MDKLNHLLKIYSNGHVIEYLTKVRHTLRPFIGDKRYSKLDSAIRHRTSRFTIVLENVGNPGNISAITRSCDAMGIHNIHLIENRSFLQDFTASQALTNDNVNSRAINSLTRQQHYIDQQQQQQIQDNNSSSDSPLYKGGYAVSMGSEKWVNVVKHTNVSSCVDHLKEKGYSIWTSDLTERAFQFDKLVFQSNNYNQLPNQFKSIFNSINNNDANLSESIYNKYLIESNSINSNSNSSNDDYIVREQEKIALVFGNEVVGVSNELKEKSDQTFFLPMSGMVQSFNVSVSVAMTLAYLKAQGALNGNLSEFEKNLIFTKWHIDSLGDSDLILKRYNIDPDQVQDYLKLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWC4</accession>
    <name>A0A8J4PWC4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_007951</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000452">
        <property type="protein sequence ID" value="KAF2070736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="20317at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057709">
        <property type="entry name" value="DUF7949"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF29">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25820">
        <property type="entry name" value="DUF7949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035258179" description="EGF-like domain-containing protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1288"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3 4">
        <location>
            <begin position="1024"/>
            <end position="1035"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2070736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1330" mass="149279" checksum="E04AC7598117531A" modified="2022-05-25" version="1">MNTPLKYILLLGISILFGLYCTDAQQQNIPTVVKIYENPSVQSSSPPYDCSRSIMVLLELNGFDIKSYELKNSTYALIRMVYLVPVSSSLAPILILRDTINTEIQISTNITLTCDALPFPLEMKEQIIYAPLTYYQDFYYLSYKIKGVGGPEGTTIYLKEEYSFFVDTPQYIYRAIKPGFDPSLITSTSGNTKLTYSLTDSFNRTTSFTIPTFFQDYQSPGQVKDIWFYPLSNKLSYGLIPIVTMEVEGNLAIPSIKRTLDGFPFSYYQYLYPVSRLNGKTQYIAALKTQFIYNQTITLGVFNSPLSRRTKVFNMTLQDDITHGNESATYTMIDKAKALFIVNFKLQNLQNRSPGVDFYISNNGTIYSERYGIGKGTELSYEFTISSLKSYQDNVSFGFAGRPSPTTLSIPVPNDNTPPQTIDITIERLPQGELLFRVHAIDDLSGVSEIEFSQTGTKLSLANLVSGTPLDGVFEIRARVTAIGDSVGYSICDKANNCKSSSSYPRPFYTIDKLVPTVPDYDSADVEYTHFSFEQNNLDLSTFGVFNTVYLNSTNVNLNSVPIISIQLTKTVGLFEASQYDTDNMMEWDPEKKLFKKTFFVPPRLFKGTVEYLIFYKSKTISSSQMYQKFKEASELRVLDSENPDEMPPIVSRIEFSPSSLTINSNQQQTIILIVDIEDPINGLDYGSIKIGTDYDSFIGYSFDFKPSDAINKDPFFGTYHFKFNINGNCRSQNYYIKSISLVDTSGHQSNTLGNIINPLFKLYDSTSLYLPVQCTIQLDSIPPVIKSLQFSKTIFNNNNLQRGYSIRLTTQDDISGISLRHNPIVFIEADDNGFDQVEMKLDSYDSNSKTANYYATSTLAFNLGSDTGLIVSVYGLYDNHLNTNGYSAEDLRLAGLLSFINTTFVRGPVIEDANPISSLGGELYLFGKNFKGTNVFAMIDYLDGQGYQTTRLFSKHTGSSLSIVGVIPKSKSFFVKLSSDAIESNEFLVIPVPAIDENVNFCFGNPVCGGPEKGYCASKIAGCVCFEPYSGISCSNKNINTDPNVQNKPDIDNEFDIFKYKISVLRIDEMDINSKAIKSHPLSDWVSKNVSTTTRKEYQYSTEIKNNDTPTPIDINIIFFDKLEQVIFANNEYYMTPNSIKYIISLGSYSFSSSLNTLQLVMATNFTINSNETCSSVSAGNSSLTSEYFKFKVNDGYLYSRFVKLGVIDKRPQPIINVILPSNDEKESNNLFSQIGIRIPFYTKNALLDPDFSVLIDSKSAKEEENSFCGKNIDDQDIHQKRLSTGAIIGIVFGCVGLVAIVTISIVIYINRRRIMVHSIKLKKFVKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWF3</accession>
    <name>A0A8J4PWF3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial</fullName>
            <ecNumber evidence="9">6.2.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">GTP-specific succinyl-CoA synthetase subunit beta</fullName>
            <shortName evidence="9">G-SCS</shortName>
            <shortName evidence="9">GTPSCS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Succinyl-CoA synthetase beta-G chain</fullName>
            <shortName evidence="9">SCS-betaG</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_004286</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + succinate + CoA = succinyl-CoA + GDP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:22120"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.2.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer of an alpha and a beta subunit. The beta subunit determines specificity for GTP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the succinate/malate CoA ligase beta subunit family. GTP-specific subunit beta subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.4" evidence="9"/>
    <dbReference type="EMBL" id="AJWJ01000149">
        <property type="protein sequence ID" value="KAF2074390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWF3"/>
    <dbReference type="OrthoDB" id="1552at2759"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042709">
        <property type="term" value="C:succinate-CoA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004776">
        <property type="term" value="F:succinate-CoA ligase (GDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006104">
        <property type="term" value="P:succinyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000002">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000002">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000001">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00558">
        <property type="entry name" value="Succ_CoA_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03221">
        <property type="entry name" value="Succ_CoA_betaG_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013650">
        <property type="entry name" value="ATP-grasp_succ-CoA_synth-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034722">
        <property type="entry name" value="Succ_CoA_betaG_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005809">
        <property type="entry name" value="Succ_CoA_ligase-like_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001913">
        <property type="entry name" value="PRK00696.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01016">
        <property type="entry name" value="sucCoAbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815:SF10">
        <property type="entry name" value="SUCCINATE--COA LIGASE [GDP-FORMING] SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815">
        <property type="entry name" value="SUCCINYL-COA SYNTHETASE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08442">
        <property type="entry name" value="ATP-grasp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001554">
        <property type="entry name" value="SucCS_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 9 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="ATP-grasp" evidence="12">
        <location>
            <begin position="38"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="81"/>
            <end position="83"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="357"/>
            <end position="359"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="9">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="9">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2074390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="421" mass="45546" checksum="E654E43726E86418" modified="2022-05-25" version="1">MSFFNSIRTPLHKTTGLLTFSSTKYQVRHLNLHEYQSKLLMDRFGVNTQKWRVINKASDAPKAASELTGELVVKAQVHAGGRGKGTFIETGFKGGVHLCKTGAEAQRLAGEMLNKHLVTKQTAAEGTLVQNVMIAESVDPKRELYFAILMDRKYGGPVIVASQKGGVDIEQVAEEDPAAIIKEPVDIVKGITDAQTKKLAEQLGFTGSKIADAQNQMKNLYQLFIKTDATQVEVNPFAETTDGKIYAMDAKINFDDNASFRQKEVFEMRDTAEEDPREVEATKYGLNYIGLDGNIGCMVNGAGLAMATMDIIKLYGGTPANFLDVGGGATEKTVTEAFKILTKDPRVKSILVNIFGGIMKCDVIAAGIVAACKQVGLKIPLVVRLEGTNVELGKKILQESGLNIVAANDLDDAAKKAVATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWN7</accession>
    <name>A0A8J4PWN7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003547</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000112">
        <property type="protein sequence ID" value="KAF2075157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWN7"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="1">
        <location>
            <begin position="5"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="329" mass="38167" checksum="E037E75E8FD051F9" modified="2022-05-25" version="1">MIKIITGNAGRGKSTLLNILVESNVFHSSISTDGSGVTKHAQVYSIGNSKFIDSPGLNDAHDKRRAAKEIKKCLQENINYKVVFVCNLESGRINDELTTVNIILNAIDMDKRNLYYGIIYNNVSKGVKDLYEKNGALLDIHLRQLPVPPREYVILERLGELEEGDNVLSSNQSFNYRLKKFIDSFSPTTFPKNSIKDIDPDTYDQIFTELEKRFYDYVQEKEEEIRILKQEVETKLNQSIKSIFEEYKQEVEKGIKKDGVVRYETTFRKVIPERITVQQKRVVDQTTYYKRLKIIKCNDTIEYTAWAEYNKMETEISIEDVEIHSEFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX11</accession>
    <name>A0A8J4PX11_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Enoyl-CoA hydratase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_004449</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000157">
        <property type="protein sequence ID" value="KAF2074239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX11"/>
    <dbReference type="OrthoDB" id="2018133at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006108">
        <property type="entry name" value="PRK08259.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43802">
        <property type="entry name" value="ENOYL-COA HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43802:SF1">
        <property type="entry name" value="IP11341P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="70"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="76"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2074239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="279" mass="30862" checksum="431D57E0E4F617B6" modified="2022-05-25" version="1">MNREIINDNILLITINRPETRNAIDRDTADKLYQCFKEFDADEKLNVAILYGGENYFCSGADLKALSSSSRNGSITTTTTTTTTTTTTTANRLETNGDRGPMGPTRLQLSKPVIACINGHCVAGGLELALWCDLRVCSNDSIFGVYCRRWGVPLIDGGTIRLPRLIGQSRALDLILTGRSVNSKEALEIGLVNRVVEKAKVLETAVELAKLLSSFPQKCMRSDRLSCYQQYDFNSIQEAIENEFKLGYNSLLEEGINGSKIFALDRQGRHGNLIIESKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX16</accession>
    <name>A0A8J4PX16_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003174</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000096">
        <property type="protein sequence ID" value="KAF2075533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX16"/>
    <dbReference type="OrthoDB" id="23992at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="519" mass="59254" checksum="59EC6F1C416D5AE5" modified="2022-05-25" version="1">MTESFYLIWRNQYIQRLIRNLVCEDYVINVNNKYLIKNHQYLSLFTNKDKLDYNITIRFMGYAVDYFDIDKRNRYLINNVDISVDSDVDNIDLNEIHDGVHKLSLIMGYGTKVTGKLPSSITYLRLYKNTFDHQPPYAQQILSNLPANLQELELGIQRYSISSPCIMPESLTDISYNTPLYYQNLKWFVVPPNKVYESCMLYVDSMESFEWLIVNKWICFIEIDANLLNMLMFQHQLPSHVTNIVVSDGIVQDTSFFPQTLESLYCCYGTLISHLAHLKYLVLVGVHELKLKKGVLPASLKDLCLDYNHPLEVDVLPPHLTILNLLSYNQPLGPNVLPPDLTTLWLPSYNQPLCSNILPSSVTDLDLTSFNQPLGAYVLPQKLKRLRLSEFSQPVLPSHSLPVSLTSLTIKGFNGSFDQCQPLDNLKELQIDSLVPSLATLLVNVEKICLRANRKVSDPSSIACLYNTSIRSLLIDFYDRTTIYPNTFPPTLKYLTLVNAVLDSDDVIPSGCVNLTKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PY45</accession>
    <name>A0A8J4PY45_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_002854</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Critical mediator, in cooperation with CASP4, of endoplasmic reticulum-stress induced apoptosis. Required or the activation of CASP4 following endoplasmic reticulum stress.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Constitutively interacts with CASP4; required for the localization of procaspase 4 to the ER.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM214 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000084">
        <property type="protein sequence ID" value="KAF2075820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PY45"/>
    <dbReference type="OrthoDB" id="19574at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019308">
        <property type="entry name" value="TMEM214"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13448">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13448:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="544"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="35"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="75"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2075820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="582" mass="67187" checksum="ECCF37F5FCCC4034" modified="2022-05-25" version="1">MTKKKASKNNNNNNNNSNDFDEEFDSGEWINIPTKEQRQQENNEKRLKKKEELQKKRDVELKEHQEKQKRIEQLFNESSKAFSGGKNKSTPWGLLDEDVDDFEQSNQHAPKKSTKQQAVDPNKGFHYGAINIENKQKKKKLTNSNNNSPKIISSTPKVSLEKAIESFNFKNYYNLLDSLEKKYPSVTDLQIKYIAECLEEHFGNVESDTAIFDSRVDYLEKFDDKFKDETKKFFKSKPISNILPTALFLIHNILSILRKDKEAINTTGVGLEILLQLIFRYFPETLLNGVSSFKSMNIQSLKPNVISLFFWFFIQPINPHISLSIWLQLIFPVLYKNKLNDQVLSLFSKFSDLVVNNEKLTNSSNKDFVDSNVISNTLQDYILTESLSKIPSGDGVKYSHILYDLDKLFPSHKIPSDYFYVLLVNAALPIEQTRTNILFNIINCIKMDKNCINYLKNGYKDNIAQVNNILLYTLLKWSHISDSKISKESIYNLAIYCKEYNEQVLGKLNQGKLKTKVNIKEVEMANITCNSVIKSFKSNKVLKYTIYFILLPILAGAGYLARQVVCEEYTSHLGQKAVEYLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYY8</accession>
    <name>A0A8J4PYY8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000096">
        <property type="protein sequence ID" value="KAF2075529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYY8"/>
    <dbReference type="OrthoDB" id="10290201at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="489" mass="55980" checksum="519BFC2438978E76" modified="2022-05-25" version="1">MTTSTTNTHRFYSLWRNRYIRDCIRNNVCRDILIDATIEYLNNNHRYLSLFSYNDKVEYNITIDFKIMNRNDVIGYVNNKHRDLIDRISIANGVDVAIDKDQQKQAPRVNRFDCNLFHQGLRSLSFYNQRTVGSGKLPDSIRELTIYSGVSNNINNPLVDMILSELPPQLKRLSLPNGLNINVPTCRLPDTLNYFNFQGTHEKLKRFVVPQNKVFKCTIGVESDQDLQWLVQHTWVQQALFETEYDATRVFPSHLTSICIHLIDGRLEKGILPSSLTSLYIYNYNEPLEVGVLPPGLQWLDLSDYDHPLEQGHLPDSLTELGLGCYNQPLEPFVLPNNLTRLTMNGFDQECFQANTLPRSLSYLKIGSFQGSFEHVGPLDNVFGLIVNSLHESVATLLQNVRTVQIRTDQISQHVSLKDTVIQDLVFYNTLDRFNLSPGFLPSALQNLEIRGFDIIMENVVPTSCISLKTDMANLNTKLLLSSVKITYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYZ3</accession>
    <name>A0A8J4PYZ3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002654</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000075">
        <property type="protein sequence ID" value="KAF2076040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYZ3"/>
    <dbReference type="OrthoDB" id="4080456at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000013">
        <property type="entry name" value="cysteine-rich protein 2-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20916">
        <property type="entry name" value="CYSTEINE AND GLYCINE-RICH PROTEIN 2 BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20916:SF26">
        <property type="entry name" value="CYSTEINE-RICH PROTEIN 2-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13508">
        <property type="entry name" value="Acetyltransf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="779"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="422"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="613"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="230"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="284"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="353"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="375"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="422"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="487"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="502"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="513"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="556"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="631"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="660"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="918" mass="100822" checksum="237478064A3CF9C8" modified="2022-05-25" version="1">MSSTPSKATAKTRARSAAAAAAAAAANPSPTTTTTPPPTTTTTPPQTTATKVTTPTTPAPTSTTTTTTTTTTSSSTATSSSHHTPTPKSEPNVNNKRTLDTPNWEVKKKPKKRKPGPLSIAEEDFIANAFKNVSDSVLTADIRRIKRKFIVKEQQRANGIQPFNIDDRVHQFITHTKKKDQKLQNYYTNDEIPFFQIKSFIPLVKTPQQPPVNPPQQHQQQQHYIDSHNHQYQQQQQQQHYTPPNSTPPNYYQQPQPQMNPQQPQQPYYNTQPVSPHHPPNPSSHPIHPTNNYPPQQQQPLKQNTPPTPQQQQLQTQPIYKPPPPVAKTVPNPITQSPPILPTIPVSTIPVNTTSPATTPRTISPPITSTPPPTTTATTPITSQPPILSTAPPTTITTNIPPTGATISPPAVSATITTPQPITPTVATVPPPTVTTTSPPIVSAPPSTIQTPKPTTIVSPPTINTSPPTATIQTPPKTVSPPMLSTALPNTTKNTKTVLPTLPAPSTPPTTPPTATTTITPKKTTITPPTITFSRAARGGGAPTVEKDLDATKPINNNNNNSTSSSTTTVNSNNNNNNDSINKLTNAKKNKPFSEIISDDGENRTIAYDLTNPPKRIEIPTPKILPKKPSVSATTTPTQAVKQSSQDSLTLERFYHWSNDDNSNNKNQKYTAQTTSSASSTPISTFKKPFQPITELIMGKFENTLLVKPYISPHTTRELKPFIRRDYETCPPKLSILRELYQFFNDHLELFNYDINAGGGGGNLNRSNTPSSPAIVHSIDYRYLTPDLVEKVNLFLCNNFWPGIDVSDILEYPDYTVVAMYRNLIIGCGLMNPDGYIMFISVHPEWQGQGIASFMLYHLTQTMVGKDITLHVSTNNSALTLYHKFGFKVEEHITNFYDKYYSDQSGKSKNAYLLRLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ30</accession>
    <name>A0A8J4PZ30_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycine--tRNA ligase</fullName>
            <ecNumber evidence="5">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Diadenosine tetraphosphate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">CYY_002614</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>2 ATP + H(+) = P(1),P(4)-bis(5'-adenosyl) tetraphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:34935"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58141"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:34936"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>tRNA(Gly) + glycine + ATP = glycyl-tRNA(Gly) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:16013"/>
            <dbReference type="Rhea" id="RHEA-COMP:9664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9683"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.14"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:16014"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="5"/>
    <dbReference type="EMBL" id="AJWJ01000073">
        <property type="protein sequence ID" value="KAF2076100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ30"/>
    <dbReference type="OrthoDB" id="57698at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070150">
        <property type="term" value="P:mitochondrial glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00774">
        <property type="entry name" value="GlyRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00858">
        <property type="entry name" value="GlyRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.230:FF:000001">
        <property type="entry name" value="Glycine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.720.200:FF:000001">
        <property type="entry name" value="Glycine--tRNA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000004">
        <property type="entry name" value="Glycine--tRNA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000158">
        <property type="entry name" value="Glycyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000010">
        <property type="entry name" value="Glycyl-tRNA synthetase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027031">
        <property type="entry name" value="Gly-tRNA_synthase/POLG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033731">
        <property type="entry name" value="GlyRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002315">
        <property type="entry name" value="tRNA-synt_gly"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00389">
        <property type="entry name" value="glyS_dimeric"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003211">
        <property type="entry name" value="PRK04173.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745:SF0">
        <property type="entry name" value="GLYCINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745">
        <property type="entry name" value="GLYCYL-TRNA SYNTHETASE/DNA POLYMERASE SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01043">
        <property type="entry name" value="TRNASYNTHGLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="18">
        <location>
            <begin position="263"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF2076100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="700" mass="77965" checksum="7655182E17C250E6" modified="2022-05-25" version="1">MIFRNSLLSSASVINKVKLQRVVVSTTTPALASTPLIRRCLFSTTNNNTINTTNTINNNTTSTTLISKLKHQLVDSINSNNMEASTFSRSGLEDLLKRRFFYIQSFQIYRGVAGLYDYGPPGCAVKSNLINFWRQHFVLSEDMLEVDCSALTPEVVLEASGHVAKFSDFMVKDELTKAYYRADHLLEGHVEKMLEDPKLTPEKIAELKHVLTKAGDYPIEELKAALTKYNVKAPETNNAISEPYPFNLMFKTQIGPSGHSVGYLRPETAQGIFVNFRRLLDFNGGKLPFAAAQIGNAYRNEIAPRSGLLRVREFTMAEIEHFVNPEDKTHPNFAEIADIKTNLLSAAAQDTTKEIEVTTFGEAVAKGLINNQTLAYFMARTQQFLHTVGIKPARLRFRQHQSNEMAHYASDCWDAEIHTSYGWVECVGHADRSCYDLTVHSKCSGTSLTVFEEFKEGPKMVDSIDVNMNKGLMGKTFKKEAKAPMDYLAALADKVDEALAIQQQLTEKGVYTLTIDGVAFNVTKEMVTLTKTQKKVAGVTYSPHVIEPSFGLGRIIYAILEHAYWVRGGDEQRGVLSLAPIIAPVKCSVLPLTTSEKLAPFVSKIIKILKEAGISNKVDDTGNAIGRKYARTDEIGIPFGITIDFTTPEDQTVTLRERDTTKQVRIPIADLAETLKKLCDNTTTWDVVSTTYPIYDSNSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZM7</accession>
    <name>A0A8J4PZM7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000552</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000010">
        <property type="protein sequence ID" value="KAF2078168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZM7"/>
    <dbReference type="OrthoDB" id="10069473at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12323:SF0">
        <property type="entry name" value="CALCIUM HOMEOSTASIS ENDOPLASMIC RETICULUM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12323">
        <property type="entry name" value="SR-RELATED CTD ASSOCIATED FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CID" evidence="2">
        <location>
            <begin position="21"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="231"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="240" mass="28090" checksum="C0BEA190091AFDD8" modified="2022-05-25" version="1">MNNYQQHHQQQQNEEFRNINNDDDLLGELNDLLNDLNPSNSYIKYAKDWILRYSNKSNLIINTIVQRVPVIEPPEKRLFLLFLISEILHSCAIEKTLDHHHHHHQEHYITLLYQNLPSILSNTYYLLDNQQKDKIKNICNLWEERKIFNPNEIKSLKSNLKDVPPLGIASPGAIVTHLKLASNVTLPLNPTDINYYHLHQFDRSANNYSLYDSLRQFQENINAPNGPVPPNTQQQPQSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZU2</accession>
    <name>A0A8J4PZU2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">DNA replication ATP-dependent helicase/nuclease</fullName>
            <ecNumber evidence="19">3.1.-.-</ecNumber>
            <ecNumber evidence="19">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">CYY_002074</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Key enzyme involved in DNA replication and DNA repair. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair by mediating the cleavage of 5'-ssDNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="19">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="19"/>
    <dbReference type="EC" id="3.6.4.12" evidence="19"/>
    <dbReference type="EMBL" id="AJWJ01000054">
        <property type="protein sequence ID" value="KAF2076645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033567">
        <property type="term" value="P:DNA replication, Okazaki fragment processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071932">
        <property type="term" value="P:replication fork reversal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22318">
        <property type="entry name" value="DNA2_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014808">
        <property type="entry name" value="DNA_replication_fac_Dna2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF433">
        <property type="entry name" value="DNA REPLICATION ATP-DEPENDENT HELICASE_NUCLEASE DNA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08696">
        <property type="entry name" value="Dna2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 19" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="11 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="19" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 19" id="KW-0227">DNA damage</keyword>
    <keyword evidence="15 19" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 19" id="KW-0235">DNA replication</keyword>
    <keyword evidence="14 19" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 19" id="KW-0347">Helicase</keyword>
    <keyword evidence="9 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 19" id="KW-0408">Iron</keyword>
    <keyword evidence="13 19" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17 19" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5 19" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16 19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication factor Dna2 N-terminal" evidence="21">
        <location>
            <begin position="637"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="22">
        <location>
            <begin position="1244"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="22">
        <location>
            <begin position="1349"/>
            <end position="1414"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="23">
        <location>
            <begin position="1424"/>
            <end position="1645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="85"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="264"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="303"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="431"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="11"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="144"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="264"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="303"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="358"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="373"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="406"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="431"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="445"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="507"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU367041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF08696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAF2076645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1678" mass="189649" checksum="3AA52974D24E7B8C" modified="2022-05-25" version="1">MSLSNNKDKQQQSSKKQTKTTTSTTTSTSTPANSSQLVDFWFQSDSQSPPLDDIFDSYKDNIVGINSIVQKKGVSSKVITPFKDKDTIKRKNSSNNSNNKAERIVTPQPLDTSKKRLTFEDDDDSSDDSIINKATLGSSKTNKAKSDEKRNDRPIKSAKKLKSDGLAISKSIIKKSSNNINNNNKSISKGDIKGTNNKIVQLPSPIQTTLQRSHSTLATNSFSQMNLNFDDSDEEFLKDILGDNNNNRNINNKDNTEMELIVDREEEKDKSDKKDLDNFDDFNGLDIDLDDLIPKPVKVNQSKNINIDSNNNSNNNKHDQNNNNNNNNNNNNNNNNNNNNNNNNNNNNNSNNNKSGLNDDDSFDFDFEDIQPKPQTSPILNSKTTSFNHNTTPSITPFTISNNNYTTTTPTTTTTTTTTSTFNRATITPNYSVNTTSFSSPILQTTTSLKKSKTPPTKASKSPSKPKPNGFKPKPLPSGFKKISPNLPPELYAKLKAEEQQQQQQQHFYNSPSPSNATPTKSQNSSFDFGSPPSFNSPFKPTGFSDEFSFSSPSGNYSISNNNNNNSDNEDDKVTLAQLLGDYTINNDSFTKHSSRDANFKNKDRYERVLVLQVIYDVTMIGIGTTLHKKTVDVFSETKKKKRTLILHGEWIFTPIKSGDIANVIGEFDSNSTIHINNQQNLLVLHPDILITGTELGGSFTCSRRSILKEQLRWTLESSTVPVFGSIVHEIFQECLKSNSFSLDDIERIKKEVLLKPSFALNLSSLGETDESASNHIDGWVPRIDDWARKFVSPNSYVTRNQIDINGEKWNLSISKLLEIEENIWSPMYGLKGKIDSTFEIKLFQYEQQVINSGTPISQKKYPKIINSKYLTVPFEIKTGKGYLQPFISHLSQVLIYTLLMNDRYNQNIELGILYYLMNQNQDIGKKYGILHPILFHRNQIRSLIVSRNLLSFFLNSNSRLVQNPSPILPRMLKDKFSCSKCYFIDPCFLYHKAIENGNAESSGLPELFQEKTSHMTTSQIKYLQDWNKMLSLESESKKHNKKLIWSRSSKFSEEQGTCIADLDLISEEDCKGYFAYKFTFNNEKLANLQMSEGEYVLASIDKKYYGLGFGSIAKITLNTITVHCREKISEPPYAIDAPSMRNSGFRFSPIIEYEDSNNMTSRQRGLGKNLAYNSSPTSGFKWRLDIDETQSSFNLVKSNLFNLFLKKNEEKSQLVVDLHAPSFNLLSDQALSLKEIGEKYPMLNIDQQKSIQKTLLAQDYSLIMGMPGTGKTTTLISLIHLLASTNKTIIISSFTHSSLDHLLLKLLQSYPDIQILRLASNISQVHANIQKYCLETQQFHNISQIKGFFEKQRIVACPSLGISHPFFSSRNSFDYCIIDEASQPSQVMSLGPILMAKIFVLVGDNFQLPPIVSNAEAAKLGMGVSLFKRLGNAHPSSISYLVHQYRMCSEIMSLSNELIYNNRLVCSNHDISSAKLPIPKRNQSNYPEWIQTYILDPNRHIVFINTDEISLAKEKSIGDHYYNTFESDLVASTLFSLVDCVDLNTNQIGIITPSRTQLKYIKTSISNKIKEQTSGTIQLDESKLDILTVDRFQGKDKDCIILSFVRSNNHQDASKAIGESLKDWKRMNVAFTRAKKKLIFIGSQKTLSTFPFYQEFFFLLKEKNCILDLPQDENKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q043</accession>
    <name>A0A8J4Q043_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002427</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000067">
        <property type="protein sequence ID" value="KAF2076249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q043"/>
    <dbReference type="OrthoDB" id="16410at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021837">
        <property type="entry name" value="CfaA/B/C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38739">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38739:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11912">
        <property type="entry name" value="CfaA_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035200343" evidence="1">
        <location>
            <begin position="19"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="209" mass="24130" checksum="30697320CE8F0F6E" modified="2022-05-25" version="1">MMKSTLFILLNIVLIVTAVTYVNFDPHKNCGGTNPNGEGIGYSWITGTCLNLDQGSYLIEIDPHDSNTAIFYFFSNPNSKCAQGEPNVTKSYAVNTCYPAQWIYDRAFYNVYNYAVMSIVEDPGYQPLEGYRYTLYQKQDTVCHADYLMYYYYTNDMKFHNTMQTFLYQCVDETPYETLCTQGLPCINSTIYFTCQNTNSNKYHYSITC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0B8</accession>
    <name>A0A8J4Q0B8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002348</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000064">
        <property type="protein sequence ID" value="KAF2076342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0B8"/>
    <dbReference type="OrthoDB" id="539213at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052050">
        <property type="entry name" value="SecEffector_AnkRepeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586:SF3">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1001" mass="116852" checksum="335471C64C1D78AD" modified="2022-05-25" version="1">MTNTTGVTSDTMSQYNGSLFYQVWGNQYLRNDVLKRLKTRVNFDVIDMDKGFGHRYKKIRDTKMRVYRSRSTAGIDRDKVMYFDDIMMTADWISKNKYFYLMYDKLMHSKTVFHLSFASVARLLIHNKDYAMFQRFYEAVPDMFYHTNRNRFYGKERSKSEDERDFLRRCNKSKKSRLKYHAGVKETIVDLACKGGSTRIVEFLVGQGYFATERGLLYAVEKGHLEVVRYIVRYCKSIKATSEAMDLAIKHGFKEIVVYLAENGVGYSAKSLRYPSEKNDGDLLEILYGLDSSKFVDNANLLLGAAKGGHLGLVQLLINEGTISKKKVMESDVMSFALSSGNKELVEYLESTFAELTIASRCLEFIASNGHLEMFVKYHKLVQRDGYRTGGYNMYYALKNRHYNILEYLIKNSLGTFDYSYRTETLLAKRAKELYSSTSLRVADILKVLLVYPGFSSSIGRIATAMSDTLGLTGVFDYFVNSSNYNYRLGELFFYNLLKGDLEKCKVIIGSFKADLLQNYEHWVELNCFESVQWLYNTFEQDLTTPKSFRYQIHNCQFKLNVVVSLDFDKQKSIQQFYYCTAKKIPLNQVQYFVFALQHGYFDIIDTLYHTQDINNLHKKNRFPKYLYERTIAAHYGVEFKPRPDILQKSPYILNYYLYLNGLTSREDFVMDKFGQIKKLKFLYYNFNNQLPYTQNIYLHAHTSRFNKQLVDFIYYNMIDRVSLTFFLNNYRIFVVRPKKYFVKINFYGEKNPKERVIYFVNQLESEPNKNLNYLMCLKRMFDKIIKIDYPSILGPYPLSLIKKYWNSFGPKENDLLWNYLSPKVASIGMSCCFLCKIAFPPFVFHQCRHPEGGLRLVGFACQLILDCFGSHKNGHNSNQEIIKVFPKGICGCTRLLLVNSSVSFGPNNSLELLIDTNFSSPVIVFKDKIKKSIYLLSLETSNNNNNINMNEDLKNKYPSSFIYTLNFGKYNLNVHLPRLPYLSIYAISLISSFKAYIYNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0G7</accession>
    <name>A0A8J4Q0G7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial</fullName>
            <ecNumber evidence="18">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">CYY_000064</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 18">
            <text>a quinone + succinate = fumarate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="16">Flavinylated by SdhE, about 5% flavinylation occurs in the absence of SdhE.</text>
    </comment>
    <comment type="pathway">
        <text evidence="18">Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Mitochondrion inner membrane</location>
            <topology evidence="1 18">Peripheral membrane protein</topology>
            <orientation evidence="1 18">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="18"/>
    <dbReference type="EMBL" id="AJWJ01000001">
        <property type="protein sequence ID" value="KAF2078693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0G7"/>
    <dbReference type="OrthoDB" id="71672at2759"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER01006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.700.10:FF:000001">
        <property type="entry name" value="Mitochondrial succinate dehydrogenase flavoprotein subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.80.40:FF:000002">
        <property type="entry name" value="Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:001062">
        <property type="entry name" value="Succinate dehydrogenase complex, subunit A, flavoprotein (Fp)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000026">
        <property type="entry name" value="Succinate dehydrogenase flavoprotein subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.100:FF:000001">
        <property type="entry name" value="Succinate dehydrogenase flavoprotein subunit (SdhA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.100">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.80.40">
        <property type="entry name" value="succinate dehydrogenase protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003952">
        <property type="entry name" value="FRD_SDH_FAD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011281">
        <property type="entry name" value="Succ_DH_flav_su_fwd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014006">
        <property type="entry name" value="Succ_Dhase_FrdA_Gneg"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01816">
        <property type="entry name" value="sdhA_forward"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01812">
        <property type="entry name" value="sdhA_frdA_Gneg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE 2 FLAVOPROTEIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632:SF51">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000171">
        <property type="entry name" value="SDHA_APRA_LASPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00504">
        <property type="entry name" value="FRD_SDH_FAD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 18" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7 16 18" id="KW-0274">FAD</keyword>
    <keyword evidence="5 16 18" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 18" id="KW-0813">Transport</keyword>
    <keyword evidence="4 18" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="19">
        <location>
            <begin position="55"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal" evidence="20">
        <location>
            <begin position="504"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="332"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="60"/>
            <end position="65"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="83"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="399"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="432"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="443"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="448"/>
            <end position="449"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Tele-8alpha-FAD histidine" evidence="17">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000171-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAF2078693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="640" mass="69837" checksum="F62560190CCCC111" modified="2022-05-25" version="1">MLSSLSKTLSKTVQKTLVSSSSNSAAISTKGFIRSFSTQQQQQARDYPIIDHTYDAIVVGAGGAGLRAALGLTEQGLKTACLTKLFPTRSHTVAAQGGINAALANADQDDWRWHAYDTVKGSDWLGDQDAIHYMCREAVPTVLELEKYGVPFSRMDDGRIYQRAFGGQSKNFGKGGQATRCCAAADRTGHALLHTLYGQAAKHDTKFFIEYFVTDLIMENGECRGVVAICLDDGTIHRFRSHATVLATGGYGRAYFSATSAHTCTGDGNAMVIRAGLPCQDLEFVQFHPTGIYGSGCLITEGARGEGGYLLNSAGERFMPRYAPSVADLASRDVVSRSMTMEIREGRGVGAEKDHCLLNLTHLSPEIIDERLPGIRETAMIFAGVDITKEPIPVIPTVHYNMGGIPTNYKGQVINHKDGKDELVKGLYAAGESACVSVHGANRLGANSLLDIVVFGRAVANEIAETLPKNTPHKPLPKNAGMESIANIDAIRFANGPRSTAEIRLEMQKIMQRNAAVFRDGPVLEEGKVLIDKCAQSLKNDLGTTDRTMIWNTDLVESLELQNLMTQAVLTMHSAEARKESRGAHAREDYKERDDANWMKHTLSYLDVETGKVTLQYRPVVTKTLDESEMQTIPPAKRVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0K7</accession>
    <name>A0A8J4Q0K7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_002232</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000059">
        <property type="protein sequence ID" value="KAF2076492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0K7"/>
    <dbReference type="OrthoDB" id="5800476at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01866">
        <property type="entry name" value="Rab2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14017">
        <property type="entry name" value="STKc_TTBK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000481">
        <property type="entry name" value="Asator, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000263">
        <property type="entry name" value="Ras-related protein RABB1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047916">
        <property type="entry name" value="TTBK_Asator-like_STKc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="14"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="297"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="297"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="334"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="386"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="419"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2076492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="657" mass="72561" checksum="6D73D4017C4BDBFE" modified="2022-05-25" version="1">MQGLASGSLIKGKWTVLKKIGQGAFGEIYSGKNIMDGEPIAIKVERLDAKKPVLRLEIAVLKKLQSTPYVCRFVTCGRHNDFNFMIMELLGENLSDLRRRQVDGKFSMATTLKLGIQMIQSLEAVHDLGYIHRDIKPSNFAIGLAPTKRSTTYLIDFGLARRFIMASGEVRPARESTGFRGTARYASINSHLSKDLGRRDDLWSIFYVLVEFAEGQLPWKKLKDKDQIGDMKMKYNTPDLVKDLPVQFTQFMKHLKSLKYEDRPNYVLLQSLLSECYSSLGITENAPLDWELAVNGNNSSSNNTSQSNLGKTGGHHNHALKEPSPSTVEVEISQPYNSNNSNVRGGGAENEMGSSQPKMLRKDSASASAMGSSGGGGNGSNKSASYRSSSPRNKGLDQSNGGDNVDVTTGSKHLKAKASSPSPNLNSQNSNHHQNNINNFQTNNTEEEKINHHGRNSSSKCCQSVGKSCLLLQFTDKRFQPVHDLTIGVEFGARMVTIDNKPIKLQIWDTAGQESFRSITRSYYRGSAGALLVYDITRRDTFNHLTCWLKDARSYANSNMTIILIGNKSDLEHKRAVTYEEGQQFADEHGLIFLETSAKTAANVEKAFESTAYKIYEKIQRGDFDINNESFGIKLGAPQAKTGENSGEKAAGGCCGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q167</accession>
    <name>A0A8J4Q167_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Protoporphyrinogen oxidase</fullName>
            <ecNumber evidence="4 11">1.3.3.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_001333</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>protoporphyrinogen IX + 3 O2 = protoporphyrin IX + 3 H2O2</text>
            <dbReference type="Rhea" id="RHEA:25576"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:57307"/>
            <dbReference type="EC" id="1.3.3.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="11">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the protoporphyrinogen/coproporphyrinogen oxidase family. Protoporphyrinogen oxidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.3.4" evidence="4 11"/>
    <dbReference type="EMBL" id="AJWJ01000031">
        <property type="protein sequence ID" value="KAF2077330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q167"/>
    <dbReference type="OrthoDB" id="419752at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00324"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004729">
        <property type="term" value="F:oxygen-dependent protoporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004572">
        <property type="entry name" value="Protoporphyrinogen_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050464">
        <property type="entry name" value="Zeta_carotene_desat/Oxidored"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00562">
        <property type="entry name" value="proto_IX_ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923">
        <property type="entry name" value="PROTOPORPHYRINOGEN OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923:SF3">
        <property type="entry name" value="PROTOPORPHYRINOGEN OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0274">FAD</keyword>
    <keyword evidence="5 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 11" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9 11" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="12">
        <location>
            <begin position="15"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2077330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="515" mass="57305" checksum="BBD0B8D3F9B05B06" modified="2022-05-25" version="1">MIHNNKRIAVIGGGISGLTASYYLMNGVGVGTSNANKYQITLYEKNDRFGGNIKTTKLDKQHCVVEQGPRSIRAAGTGINTLDLVNRLGLENELIFASKKSNGKFLLLDGEIQELPSPNKLLKSIQFVIGQGIVSSFLAEPFKKVPKDVVDKPDFDESIHSFFKRRLGSRVADIFVEPMCLGIHGGDYKKLSIRACFPSVANREISNNSLLGLNLFGEKSDKTISTIPQQLPFLLPEKQSIKEILDKDLDKTGVFSFENGLNTLISSLVENLRANGVKLNHSTTIQEIRTNDNGKGYKIIDKDGNVEEFDQIISTIPFTSLYPMFNKSDKALEALLRSIPYTSISVLNLVYKNNASVADKIKDKGFGYLVPSKEKVPVLGVCFDSNTFPEFIDSDKDSIITVMIGGNQGIKDRNAEWINVDAKDKDDLLNIAKKHLKKYLDIKDEPDWTNVEIYQNGIPHYEVGHQQRIRSIQSYVEKNYGDKLVLGGNALDGVSINDSIFKSKSLVYNLLENNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1A1</accession>
    <name>A0A8J4Q1A1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000761</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000015">
        <property type="protein sequence ID" value="KAF2077961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1A1"/>
    <dbReference type="OrthoDB" id="19065at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.20">
        <property type="entry name" value="Hcp1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036624">
        <property type="entry name" value="Hcp1-lik_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053165">
        <property type="entry name" value="HSI-I_assembly_Hcp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008514">
        <property type="entry name" value="T6SS_Hcp"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36152">
        <property type="entry name" value="CYTOPLASMIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36152:SF1">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05638">
        <property type="entry name" value="T6SS_HCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141452">
        <property type="entry name" value="Hcp1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="344" mass="38312" checksum="8C828469D55F098B" modified="2022-05-25" version="1">MTKAIYLYLPIDVNFRNPNYQIPTIENQKDLLCAGLNTSASVPPPTTPTNAPRRTHMMDDDEKKVLSAIHDGWMDVMSFSHNVAAQNKRGRGDNDDDAIHLGELSCTKFVTADSPSINYLCLASQILPHAIIRSVGDVKNNKVNIIEYELEYVQITNVSLSGGAGGKPVENLNINAKIIKTKYIDMDILSSEFQVSQVYKWDNINKTGSKTVHSTKGEPTKTLLEYAKKTLMVNLSSHDPSMVALIKELGLVTDYDLPEVKPSSYKIKVLSNSAPLVYKVIQVDSFDKKSIVSAIAEKFNIPLETIESIYSQEDHCEIEADQSFNSVVPTHILFRTSDGKFYGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1B9</accession>
    <name>A0A8J4Q1B9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitochondrial processing peptidase alpha subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000715</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000014">
        <property type="protein sequence ID" value="KAF2077991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1B9"/>
    <dbReference type="OrthoDB" id="10251424at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF222">
        <property type="entry name" value="MITOCHONDRIAL-PROCESSING PEPTIDASE SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="1">
        <location>
            <begin position="152"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="2">
        <location>
            <begin position="303"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="588" mass="65097" checksum="8EF25E50DDA0182E" modified="2022-05-25" version="1">MNNILKNNKASLLLSKLLVKPNNSSTSTSNSNISIFSQQQKNTNNANTINTNHLSQFSPSLSSLSSTTTTPSPSSLISNIFRKKNVLSYFSTSTSSNSVQDVLSKSPFLPLTVPHPKLKEFNLSNQNDNNNSGNENMDYKEQITTLPNGIKVISKQTHEEACAIGLYVNTGSKFETPENRGVFNLLQKMTFKATKNNTTPEIVRSLEDISLNAMSVSSREIMQISIEVLRKDVENILKIFSDQIKCPLFEQEELKEQIESCIRNWESLNSSADSLLPELLQRVAFGDEGLGSSIFAAPNEYESITREKLFEAIERYYVGKNIVVSGTGINHGELVQMVQKYFSDIPYQRANTPTIESVDAKTVYNGGTMAFSPESDEVDPSWIVAFPFKGLASITESNDIFTAFVLQSILGGGSAYSTGGPGKGMQSRLNLNVVYRLHKIKHCSAFFSVFNQHSLFGVNLNTTPGSLGNAIELLLSELLFFNQNVTMEELNRAKRSQKSQILTNLELRGVLCDDMARQLVGMGQWKTPEEVCKAIDSVTMEDVKALYTKIMQSKPSVVVLASEKDSVITAEDFNLIVQQNLKLLTGTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1C5</accession>
    <name>A0A8J4Q1C5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">3-hydroxyacyl-CoA dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002623</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000074">
        <property type="protein sequence ID" value="KAF2076059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1C5"/>
    <dbReference type="OrthoDB" id="1274115at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43658:SF8">
        <property type="entry name" value="17-BETA-HYDROXYSTEROID DEHYDROGENASE 14-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43658">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="255" mass="27456" checksum="57A6ABC07212CE71" modified="2022-05-25" version="1">MKISQKTFIITGGASGLGQATVRYLHQLNANVVIFDLSLESGQELVQELGNERVLFCETDITNENSVKSALEKTVEKFKQIHGVVNCAGIAVSIKTITNRGVFPLDVFSKVLSVNLIGTFNVIRLTADIINKQQSVDGEKGIFIMTASCAATEGQQGQTAYSASKAGIVGMTLPMAREFAPLEMRVMTISPGIFATPMVEALPEKAIKAIESSIPFPARLGKPAEFAQLVQTIIENPYLNGEVIRLDGGVRLAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1G5</accession>
    <name>A0A8J4Q1G5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000617</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the csb family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000012">
        <property type="protein sequence ID" value="KAF2078066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1G5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008601">
        <property type="entry name" value="Dicty_CAD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05720">
        <property type="entry name" value="Dicty_CAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="111" mass="12234" checksum="1928BF9EE066F472" modified="2022-05-25" version="1">MNQVFVSVSIELDGKHVISGNGVALPTPPIMPRPCFLMITGPVKTEGNEGVWSGRKDVKKLVLNYQGKSYPVGDCTIDVLKEEFTDDGIVDYRINILPKDNVEVPHYGQGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1K4</accession>
    <name>A0A8J4Q1K4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sodium/hydrogen exchanger</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_005976</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000255">
        <property type="protein sequence ID" value="KAF2072716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1K4"/>
    <dbReference type="OrthoDB" id="196264at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098719">
        <property type="term" value="P:sodium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004709">
        <property type="entry name" value="NaH_exchanger"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00840">
        <property type="entry name" value="b_cpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF128">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01084">
        <property type="entry name" value="NAHEXCHNGR"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0050">Antiport</keyword>
    <keyword evidence="6 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0915">Sodium</keyword>
    <keyword evidence="8 9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035319245" description="Sodium/hydrogen exchanger" evidence="12">
        <location>
            <begin position="26"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="133"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="170"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="198"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="295"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="332"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="387"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="420"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="454"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="485"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="13">
        <location>
            <begin position="121"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="61"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="594"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="63"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="594"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="641"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2072716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="653" mass="71765" checksum="8849054CA059EBE8" modified="2022-05-25" version="1">MKNSSLKWIATIVLLVLAITAAVSCSPSSVATTTKTKEAGDDEIKIHIPNFVGIGKVNTDKTGSSSNGESASNSNSNSNSNSNSNSNSATNNNNSGGALANQQDASIIIFMILMILTGAVMIVYFMVSIHIPFIPESIAIVVYGVILGLIFRFSHSDIVSHVATFDPEKFFLFILPTIIFETGFSLPKTDFFKHISGILMLAIFGTFITFIVVGLGIFGVGHTGLSIYLPLRDSLSFGAIISSTDPVCTLAIFQALNVDPTLYMLVLGESILNDATSIMLYRTVTSVGPFTAKMIFYNIALFFLVAIGSVGLGIGMALLLSLLLKWINIGKFPALETIFMIMFSYMSYVLANALDISGVLAVFFCGITFNQYGAYSLSPYTKLTSNQLFRTAAFICETCVFIYIGMTFSFHDFTFNADLFTWSIFFICLSRAMSVFPICFILNKFLKDKISIQIQIAIWFAGLRGAFAFSLGLDYESDYAKIIRTSTILMVIFTIFVFGIGTYPLLKILGIKTSSSDQSLDNINKPLNKKSKAKDRSQLYQSVDDKYFKPWFRKKVPPLATEAIEIFEKLVQSSKDMEMGSVYDFDDDDDYKNNDSFSINNNNNSEFEIGVMDDDDDIPTSSSSSSSKAKSSESKSLINQKSTFSSSSSHSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1U8</accession>
    <name>A0A8J4Q1U8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fumarylacetoacetase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_000466</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000008">
        <property type="protein sequence ID" value="KAF2078276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1U8"/>
    <dbReference type="OrthoDB" id="411064at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018773">
        <property type="term" value="F:acetylpyruvate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.850.10:FF:000003">
        <property type="entry name" value="Fumarylacetoacetate hydrolase domain-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820">
        <property type="entry name" value="ACYLPYRUVASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820:SF7">
        <property type="entry name" value="ACYLPYRUVASE FAHD1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="3">
        <location>
            <begin position="13"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2078276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="221" mass="23942" checksum="E8871E9D6518D4DA" modified="2022-05-25" version="1">MSGINKFWTTGRKIVAVGRNYVSHAKELGNEIPKEPFFFLKPTSSYLLPNSGAIEIPSSESDIHHEVELGVVIGKTGRDIDAASAMDYVGGYTLALDMTSRDQQAKAKAKSLPWTVAKGYDTFCPISGFIEKDKIKDPMNVELWLTVDGETKQKGLTSDMIFDIPTLIQHISSIMTLEAGDLVLTGTPAGVGRVLPGQVVKAGITGIVDVSFDVALRQKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1W7</accession>
    <name>A0A8J4Q1W7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000937</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000020">
        <property type="protein sequence ID" value="KAF2077750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1W7"/>
    <dbReference type="OrthoDB" id="5971719at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="94" mass="10825" checksum="4782473BED65653D" modified="2022-05-25" version="1">MSVYQKSVSHAPATLARNDTVAEDDDWETDPDYANDISEKDQRWGSKETQKQELGNMDELRNKVVAQDDRAVKEEYEKKKMLYGGERAALSKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1X1</accession>
    <name>A0A8J4Q1X1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TPR repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005892</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000247">
        <property type="protein sequence ID" value="KAF2072791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1X1"/>
    <dbReference type="OrthoDB" id="21278at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="70"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="398" mass="47135" checksum="5433F444034B7419" modified="2022-05-25" version="1">MDYVSDCQQNNNNSNNRNSNRIDDILNRTNHLDHIYINSSSASNRIVYQEFEMQHKQDGGGDDGDDDVLLKQQQQQQQQQDSIDNIEFDQDEQYCNYKQGSIVDNDQINSSFNPFDNNNNNNNNNNDQDDDIGQQKEIEGKEDDNDDDDDDEIDQVKEDDEINNSSNSRNSIYHYQQQQQQYYRHPFSYSSLLMNVGLFMLFIAIFIVNDSLKENYIMKKDSNANQYQHRQQQQQQQQQQQHQYSAYYQSLLKRETDLKDLLDKYEKSNKLGDLDQSLSVLNSVIRLSPRYCAAIFRIAQILNMKTQFENAENMYIRALECERWLKLEYIIELVLFYNSNERYQESYALSSRAILFFPNDYHLNKILNSTKDIILNRQYGDVNLDYQGRLLPYFSAIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q284</accession>
    <name>A0A8J4Q284_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005791</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000240">
        <property type="protein sequence ID" value="KAF2072896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q284"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2072896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="71" mass="7939" checksum="042AE833E909EC80" modified="2022-05-25" version="1">MSSLNTLNIISKSSKMLLNQVSPNMISQMTMPLINGNNKQIKSSFINTNNDMKLRSSLKASIEIPELNQEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q298</accession>
    <name>A0A8J4Q298_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mediator of RNA polymerase II transcription subunit 18</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Mediator complex subunit 18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MED18</name>
        <name evidence="8" type="ORF">CYY_005768</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the Mediator complex subunit 18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000239">
        <property type="protein sequence ID" value="KAF2072911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q298"/>
    <dbReference type="OrthoDB" id="10018982at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.320.10">
        <property type="entry name" value="Hypothetical Protein Pfu-838710-001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019095">
        <property type="entry name" value="Mediator_Med18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13321:SF2">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13321">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION, SUBUNIT 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09637">
        <property type="entry name" value="Med18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="18"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2072911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="287" mass="32027" checksum="03D6BB8775A9551E" modified="2022-05-25" version="1">MQNYNKAGGGGGGGQHSLPYQTKPQVPNTSGQEAYFNTLNNTQGKAGASNIYECSLHGILTTPLSTFIQRVKGMMRCEAPVNYKEMVFKTTIQSAGPSWAEGSILPSEIHIRYEKNTVYVRYIGSPQVKENINAMIRNVIDIKGNQSFFTYIENLGYVKDYEYFVEGYCYSTYNLSLYLVNHKRVLADGTRGDAINKHSMVELQCLSGEEGFIAAAEHLNTYAEYLYPFVELIKFDHRNLVTDPIVQPQQAPPPSQIPGFPAHMMTMPGSGGQPYQIGQNPYTNSNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q365</accession>
    <name>A0A8J4Q365_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_001520</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000036">
        <property type="protein sequence ID" value="KAF2077199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q365"/>
    <dbReference type="OrthoDB" id="20712at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14704">
        <property type="entry name" value="bZIP_HY5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47416:SF8">
        <property type="entry name" value="BASIC-LEUCINE ZIPPER TRANSCRIPTION FACTOR E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47416">
        <property type="entry name" value="BASIC-LEUCINE ZIPPER TRANSCRIPTION FACTOR F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="9">
        <location>
            <begin position="324"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="29"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="154"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="236"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="458"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="511"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="349"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="236"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="300"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="475"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2077199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="535" mass="60631" checksum="B3CAA1738816DBC7" modified="2022-05-25" version="1">MSLIEPDIYNLFPNPDLAIDFTSGLVPDSPNKVGQKKNSFLNSPPIKHQVNGQQQSNQQNQQNQQQQQQQQNVQMMNQNIYMAPNNMVMEEQLFTMFLSPEKDQSINIQNSIDVNNNNNNFNYGTNKNIEEFYNTGGVVPDASMNPNLYKDQQQQNIKIQQQHQNHQHQPQQLHQQQQIQQQQIQQQLQQHQPPHLQQHQNYIVPESVFEIPFSLSSMNNMNQHQHQHQQMMNNNINIQNNNNNNKQFKQQQPQQTQIQPNQSLIPIPPAPTSNNNNNNNIKKKEEDKTMKKRKFITSTPVKGENGTTLIPTPDGSGNIEEERHMKRQRRLVKNREAAQLFRQRQKAYIQDLEKKVSDLTGTNSEYRARVELLNSENKLIREQLLYLRNFVTQAVSFSFPKGANGSSSPSDILGSMPGMNSPLPQGILPPGMMNNSMIMSAIAEAASKNSAFRQGIQGTLLGTPIPSPSENANIGSPTNSSSNSTSNNSSGNNSPMKNNILNQQQVPALPYLSQSTPPQQSNMNNNHHSPLSNCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4W2</accession>
    <name>A0A8J4Q4W2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000401</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000007">
        <property type="protein sequence ID" value="KAF2078309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4W2"/>
    <dbReference type="OrthoDB" id="19768at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037830">
        <property type="entry name" value="ZZZ3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22705:SF2">
        <property type="entry name" value="MYB-LIKE PROTEIN Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22705">
        <property type="entry name" value="ZINC FINGER, ZZ DOMAIN CONTAINING 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="253"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="209"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="495" mass="54651" checksum="42A2F817D0F84C4C" modified="2022-05-25" version="1">MTEQTIKDLLKLNKDEEFQYFQVSKTDGNLGSFRKWDIDEHIKFTNALLVYDHTKSENIPVIQSLVNQGNSILRTLDQINMHIRVYQNHCIRQQSQLRANQVFLNTLASNSFQHIMDSLDVQNNNNNNNNNNNNNNNSNSLTPNPMTQSFLNNGNPLLNPLFQQNNNNNNNNTSVLPPSPTAVGPSNPSLQLASRPMTPPQPSPPLVGKKSPASTTKSTTATVTTTTTTAASIDQYQDTSSTGNISAAANKKNLKLLKDPWTNEDQKKLEDALKKYPSTRFSSVSRWQAISKELGISPKAVALRYNQMLDNGIPKKDTPVADQQIPPAIPKEASPKTTGKRKTSATKEPATPKPPSKKLQANVPLSPNATAPLPLSAPTSVPNTPISQEIINQTEQLIVKNNQLIEEIKEKLMSGQETNIDTLKSFISNIELSLKNTSNWSQNGNEMPPLPLKVNDMVLSLLAPTTAGSFSSLKKPLSKQASEWNFVLPMEDTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UNP7</accession>
    <name>A0A8J4UNP7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010439</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001113">
        <property type="protein sequence ID" value="KAF2068236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UNP7"/>
    <dbReference type="OrthoDB" id="24428at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="175"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="186"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="210" mass="23665" checksum="A90B40B5FB195248" modified="2022-05-25" version="1">MKEFPILPSSLTELKMLSTCRFESFSSPSPLHNYFPNTIVKLVLQYSGELFIGLIPTSVTDLKLHYSKPIPKGAIPNSVKKFHLVNMSKVKPIVIPNSVTYLTFEFDIQLYPELEIPDSAKTLKIKFLEFNNTPFLNYLKTPLKNITRFSTHEYMDNRKKGSLEAIAKAASNIATDAATTGAGPIPEDDEEKEEENGVECSDSDEEEERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPT2</accession>
    <name>A0A8J4UPT2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytoplasmic dynein light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009006</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000616">
        <property type="protein sequence ID" value="KAF2069676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UPT2"/>
    <dbReference type="OrthoDB" id="10059120at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21455">
        <property type="entry name" value="DLC-like_DYNLT1_DYNLT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.40">
        <property type="entry name" value="Tctex-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005334">
        <property type="entry name" value="Tctex-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038586">
        <property type="entry name" value="Tctex-1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21255:SF4">
        <property type="entry name" value="DYNEIN LIGHT CHAIN TCTEX-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21255">
        <property type="entry name" value="T-COMPLEX-ASSOCIATED-TESTIS-EXPRESSED 1/ DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03645">
        <property type="entry name" value="Tctex-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="12368" checksum="830AD2E40CDBF537" modified="2022-05-25" version="1">MTDSNFVQEDVLAILKEAVDASLSNSSIYQHNKVPEWTSRVIDHTLKKLSEGNKPFKYIVNCLIFQKTGAGFHSSSSCLWDSTSDGVCTYRWENKNMYCITTVFGCRCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UR16</accession>
    <name>A0A8J4UR16_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000371">
        <property type="protein sequence ID" value="KAF2071476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UR16"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36812:SF9">
        <property type="entry name" value="MYB-LIKE PROTEIN X ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36812">
        <property type="entry name" value="NEUROFILAMENT TRIPLET M PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="136"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="239"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="578" mass="67201" checksum="4FF8687C6263FAD2" modified="2022-05-25" version="1">MSKVTLKRPLVDNHSSPSESHSDIDVDVDRGEVFKSSKKIKTIDLDADEEDDNNDQDGDNYDEVQSVDSVILDEATKNNNTKQNQNDIEDLEVNQSSDSDQNNINNVNNKSNSSSTYNDYNSDSDDNDKKIEKSRVTSTATTTTTTTTSTVNTNINGTNINSDVENLKEKSYDENWREQFSLDIISLLKNDIEQGKQIYELQKQIQLDQIKYHQLQLQYQKQIKDLQSKISYLEHEIKNNNNNNSNNSNSNNNNNNNNNNNNNNNNNNNNNSQNKNNQKNNNKQEIDLNIDGLSSSFESEKEKLEIKYKEKKKEEEEEDIPLYMTYWEEGILLNKSFLKIFINWICNSNLLQDSSIPEIGSKGNPDTIRSLLAFKYCKNLEDRSSQYREQYRKLKGEDLMDVVFKKKIFQTKFYSSKEKLETLFDIQFDNNSLKMHWVFLINTLAELVKYNPYKKAKSYNVRDRETATKLTQVFTDIYQSLFTAQESFIIDYLSTLRANLLCKISTILDPNFLHSVSFTNNMKALDQKHKHHIEKFILSISTSKIGFMKNWLESYPSCHDRFFIECCKYALTNIGKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4URF3</accession>
    <name>A0A8J4URF3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006566</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000309">
        <property type="protein sequence ID" value="KAF2072121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4URF3"/>
    <dbReference type="OrthoDB" id="14339at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37332:SF1">
        <property type="entry name" value="ELMO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37332">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="339"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="396" mass="43893" checksum="8C5CA5DD337932C5" modified="2022-05-25" version="1">MDAIVTKMEETLERKKSIANIVEKRTMVIDYIKRIHEAAYYAGIGTSASMLTNSNSTSSVGGANGGATSGISSTLNTSNSSLSTSNSTSSLSSLNNSSGNLANIANNNQNNNNNNNNNNTIKPIHWMNVAMIQKTDFDSMIQKSDLEKNPEIQKRLLGWFCLGFSLSPLTTADKGPSYIRSLLQLIEEYDYHFELGSAMQGVKVLLSKKQPTHTVVNENEHIKAKIGKIGTTVVYEFLKIFNISIAKDLDYFHVVFSLLEILEQIYKKMDKETCSSKYVYEALLKIDSRLKHHIFGFLAKEIDKMALQIIKDQLDFTMISTSIKDKDIQQHNFNVQSKISNNVNSSNNNSNNNNNNNSNIVVNNNNNSNGTNTKDNLNTSNNNNPTSSSKGSISSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4URQ5</accession>
    <name>A0A8J4URQ5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">CAAX prenyl protease</fullName>
            <ecNumber evidence="14">3.4.24.84</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_006120</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Proteolytically removes the C-terminal three residues of farnesylated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 14">
            <text>Hydrolyzes the peptide bond -P2-(S-farnesyl or geranylgeranyl)C-P1'-P2'-P3'-COOH where P1' and P2' are amino acids with aliphatic side chains and P3' is any C-terminal residue.</text>
            <dbReference type="EC" id="3.4.24.84"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13 14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13 14">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the peptidase M48A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.84" evidence="14"/>
    <dbReference type="EMBL" id="AJWJ01000268">
        <property type="protein sequence ID" value="KAF2072571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4URQ5"/>
    <dbReference type="OrthoDB" id="360839at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07343">
        <property type="entry name" value="M48A_Zmpste24p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2010.10:FF:000002">
        <property type="entry name" value="CAAX prenyl protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027057">
        <property type="entry name" value="CAXX_Prtase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032456">
        <property type="entry name" value="Peptidase_M48_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10120">
        <property type="entry name" value="CAAX PRENYL PROTEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16491">
        <property type="entry name" value="Peptidase_M48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 14" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 13 14" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="304"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="338"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 1 N-terminal" evidence="16">
        <location>
            <begin position="65"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M48" evidence="15">
        <location>
            <begin position="223"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="291"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="12">
        <location>
            <position position="372"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR627057-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR627057-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU366005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2072571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="442" mass="51928" checksum="954D35B223DDD346" modified="2022-05-25" version="1">MVVFNIDPLLEAMPWFKFTIGCNLFIFFLELYLDIRQSFTLREFKDPNIIWTETKYHIQNIIGLLNFSRTESTIGFILECLVLYFGVLVYLYNANSYIWDHLGFNNTFQITRGVSYLLVISLVSSLTRLPFEMYRIYIDQFVVDNTTKQKVNWIRQIIWDQIKMFLISLFIGIPMLSIVLSLFYWQYPFQWLIIVVFVSGFALWFSDLYPSIAYLFNNFSILEDGELKNEINQLCLKLNFPISEIYTMDGSKRGSHSNAFLMGFWSSSFVLYDNLIKQLSTKEILAIIGHEIAHHKFKHTWKHLLIQLVFVGNFIYLFSSVVHLNVFYTGFGFKRVDVSVGLVLFSYLYSTFANLLRFVTNMIRREFEYAADAFAIENGLDMKQALVSMHGKGVYITPDRFFSLYYYSHPTLMERLESIDTIIKTLNTDNNNTIVTEDKKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UST1</accession>
    <name>A0A8J4UST1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytochrome c oxidase subunit VIb</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_004726</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000172">
        <property type="protein sequence ID" value="KAF2073981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UST1"/>
    <dbReference type="OrthoDB" id="16284at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042775">
        <property type="term" value="P:mitochondrial ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008535">
        <property type="term" value="P:respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.140">
        <property type="entry name" value="Cytochrome c oxidase, subunit VIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042289">
        <property type="entry name" value="COA6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048280">
        <property type="entry name" value="COX6B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036549">
        <property type="entry name" value="CX6/COA6-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46690">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46690:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02297">
        <property type="entry name" value="COX6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47694">
        <property type="entry name" value="Cytochrome c oxidase subunit h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2073981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="12201" checksum="1AACC9763B1C2874" modified="2022-05-25" version="1">MSNPQQPPMKPLGEMTSEERTNYSIQRAPDYSGKQEAVLTTSRTKCWSARDQLFECLDKNNDQINLCKDFYQVFSSVCLNSWADYFMKKRAADKNKKEFLERTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UV75</accession>
    <name>A0A8J4UV75_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_010303</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001028">
        <property type="protein sequence ID" value="KAF2068370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UV75"/>
    <dbReference type="OrthoDB" id="21583at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035324984" evidence="3">
        <location>
            <begin position="25"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2068370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="264" mass="28609" checksum="1DA1B884F9022082" modified="2022-05-25" version="1">MLKNQTLLLLFGIILLCFVVSTQGLDPPPPIPTSNNNSSTTHNNETIIDKSTGAETKDPKDPNHSEETNSSMNSKETNSSKGSKETNSSKDSEETDSSKGSKETDSSKGSKETDSSNGSKETDSSKGSKETNSSKGSKETDSSKDSEETNSSKGSKETDSSKGSEEKNSSKGSKETKDPKDSKDSEYSDSKSEELPQATNHGKRDSKDPYKLSSSFISGTVVVYVIGGCCMIFLGYLLWNRMKKKKPTNSRSRGQDYIALNMRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVD3</accession>
    <name>A0A8J4UVD3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_010140</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000942">
        <property type="protein sequence ID" value="KAF2068535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVD3"/>
    <dbReference type="OrthoDB" id="21537at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF29">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035278691" description="EGF-like domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="4">
        <location>
            <begin position="425"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="3">
        <location>
            <begin position="640"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="2">
        <location>
            <begin position="789"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1159" mass="130305" checksum="9A13FD10DD36264C" modified="2022-05-25" version="1" fragment="single">MKRIEILMVIGLLSLCLAQSDILMTDYTNPDNLNQYSFATVLDPVCDFIYYLMFSQTVDQGIIKVTQSSDTTGVKSSTLISQSTNKFLYQVRIRVPVGSGVSQVEAFNDLFTKQFNVAYSCEAPPSTLDIEFYNNNTLYDNGDTNTMGPSLTFKVKNFLKSPISSMLTATITSQSLVLVANVFLISDNIFSVGLGYNDAITNWSDFYNDFPISLNYLSSTLAPPSNHIIKSNYNNTNDFQRMDYIIYGLSPSDISPNRHIYPYVEFKTNYNRQPFWGIQKQRVPIVMGNYTNGLVISKITLNVAQQVNILLSYTDYPRVSMITSRVTFNIVSPTYLLTLDGINLDNGILLSSVNTNNPMIEVQFKIDSGFSYYPNTLYPYGIIAGTSASYLRNDEFYVSPYTSGQRSFYYNPQLNDFTFPTPTYSDVMPPNIESIENFIYGNIAVYRVHITDDLSGFYEIKGLGNYTSLVSGTLQDGIYEFKVDLEDKYFITVFNFEVLDTVLNRKILDMTTFMATDGSVFQIREFKKLSDINRVYWKYNDIDVSQQGLDNIFYICSSITNLKPHISHSKEWISFPFINAILGIYNNSNGCYEFNVPIHKNFITGSYDAFLWIGINWRQFSYFMSVFGEESRLRVSSINGDALGPLITNIELPSNNVQIQSDTNITWRFTIQDNVNGFKSGIVSIISQVGWVRYNFTLTPTVEGHLVLGDQYNGIYEINVPINVTCVSQKYSIYYIELKDEFGYSTLFNTYVVQSNTLIRNPLMKFYDVDAVSSISTNCVSPPQDIDPPILTSFDFSPKTIDSTFAGELLQNTSRMVTFDFQVSDATGILKESHPVIYLSDQNYNRIEQFSELVSYSITSATYRCKFNIPFGYGFPHGIKVSVYGFVDFNNNIGGYSITSLGTTYSNTIDVVQTMNSSMAILSTSKLYPDLDFYVMGTNFDINDKLIVQHENGTTLYSLDSISNMVTISQFKMGKLKGSFIYITLQRERLPSGFYYSNTFRLPVIYTEPDNESSSTSSSSDSVFPPQQCPNLCSDNGECTANGCKCKSPWIGSDCSILPTNAPQQCINKCSGNGECTPSGCICKPLWIGLDCASKVIIVNPTINTTLPSTNITIPTTSSSESVLYAIISIVQLNELNNNGDIINSYPFTQWIASNNTNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWP6</accession>
    <name>A0A8J4UWP6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_008932</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000601">
        <property type="protein sequence ID" value="KAF2069748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="427769at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035188839" description="EGF-like domain-containing protein" evidence="3">
        <location>
            <begin position="28"/>
            <end position="2473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1311"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="6">
        <location>
            <begin position="412"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="5">
        <location>
            <begin position="780"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="4">
        <location>
            <begin position="1074"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="6">
        <location>
            <begin position="1729"/>
            <end position="1819"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="5">
        <location>
            <begin position="2094"/>
            <end position="2209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="984"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="990"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2069748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2473" mass="273558" checksum="ADF9209EF7718B56" modified="2022-05-25" version="1" fragment="single">MYFKMNRYVVVTVLCVLLFVCCIKTEAQVPMPIKPIFQFGVEPYATSGVCHFNFVIEGIMMDDISADSLNSCEISNTNAATPLCSFYHLGNSSSSFGLVIETSIGSSATPSTNMVLKLILKDLSFKSFFLNSDKNVPFSYSCEEQPTLSNTILSNSPAQLLYSSNRINTFQFKTYLTLNSQINRPLGSQFACYAPGMYCVFKEISVGLIKVVATFQSTFTEAFPETITIIPQYYTIATPLTSQINSFQFLNQNSFSFKNNPALGYRFDLLTSSTIQYIPPIYAGGVPLEGQSFGNFSFYYYNLDKDNCYTIFGNKILNNGVISKSSVSAPLTWLSNTGGLIRITIQTQGSTLVFTTSTSFKQFALPYPFGVSGAKGDYAISFMTSSMVSNGFYVDIYTPDRHAALGQTYIPSDSINPLLLSLQVIVLNSTHSVLKVGASDDSSGVDSIILYFPKGPVKYVMTMENLVSGNSLNGVYEIIVPFKKKFLSNISVQDVAGNSIGLSQPQLDYRFGTGSEYPIVNTKITEFYFSPLQVDVAVSAQDVVLYANLSQTYQERMQDNSISLQINFNPNTNNPLIPGIYDDSKKLYAFPFRVPEKMMASELDFYLILNGVIYDRDYFQGPQGTLTIKNNGQFDMMFPICTQVVPSNPPILRSGNLVNGVGNTITLAWDFEFYDMTGIKTIIIGISGEYDVQGKNFTIDGVNGKTHHAFTLEWEHNSTTCRDQHYEVNYMYTEDILGNKGETIRYSNTNIHPYYLIDSSLDRNVLSGCETDPSTDTNAPKLVNLNILYSVLVNSKDVFLATFTIDDEGGAGISESHLPSCYLESNANLYLGVESTIFTRHSNGSITYRCDITMPISLNPLDHYLSIYGVYDLNYNVASFTSYDIQQLGFYNVAPFPAKKLLFIESTSSLATSSEILYIYGNGFYIGPDMLVDIKMDISSITVSPNITTGSVLVLYNLPPSFEYKVNVYDKSTSISSNIITIKGPLSSPSTPPPTSSPSTPPPTSTPSVTPSPMSCKSDCGVGQGYGKCVNGGCVCVAPRSGIDCSSIIDTKPVIKPDPVKPSVNVTIPGTSESIKDPEFISFIYVVALRELDAGSNPIATYQFNSDKWILVNEGSSTNEQVSTVQYKYLIDNSYNTTIISTVQVFAQATNITFGNQQLYMNPSTIKFTFNVTSYPFSKSTNSLQLVMTASLQSTEKVACSYKEFVDDQNNSQYLKIQIQDRSLFGRFINFGMIDGREQTISNTILDASYGGKELSKSTSDQSYIGLNIPYYTKYALLDPDFSVLVESRSAKDQANSICTNESKKLTTAQIVGIAVGGGVFLFIIGAGIIYLYTRKSTSPIAMKLRRLGGEQYASASGSCAIEFVMRGTTINFEIDSITLCTLSSDIPFSGAPTCHPSTSGSFNSSDFLITITASFGTNASIYKNINLKLYFKDSSSKTFILTKDGSTPLSYSCQQKPDVHPTIVQISGQPQLIYSSYQVKTFHFTTFLNLNPQLLKPIQDPIICLDPSFVCSFVYVKLNTYFALCSFLTTHTGELPTYVRIGLNSTDFIQMTLPTIQYKSLMASFNELESIYNAPYAGYTFNLLTNTSSQYIPPILLPLIPLHGQGFNNISFYFQNYFYDSGVAILSNTITTVFPVLNKTSNSAPTYGYTSNSDLVEIILTTNTRFNVFQTPPTNYYFHSYPFGLVSGANTQRFYRFSMIKEQMQKSYQVLIGTPDTHLVADIHASGTDSAPPTVTSMETIILNATHSVLRITAADDISGVSFIVVKTIMKIYLLTPANNLVKGNLLNGVYEITVPFKRPFVTLSIMDIASNINTKYEEYGSFFYLGAGLLENPIDRISTFYFNVSQVDVATSSQDVVLKVNISQTHQQRTQETTMFLQINFNPYLSNNPLIQGIYNPTDRLYEFLFRVPEKLLASKLDYSLFVNEFKIDSFNFEGRYGAQGILNIKNNGAFDMVFPIVTRADPVLPEQITVPPGKSFVAWDFEFTDVTGIKKVIIGITGEKDIQGKNFTVQGINGQADYSYRLEWIFDSNTCLNQKYWVNYVYTEDVMGNKGESVRWTNNGMHPYYKFDDDNTDYFDVLGCTMDPVADKTAPTVSNITFNIQEAIGTDSINITFTAQDVSGISNLHIPVCYLNPDANQFISAKASILGGSKTGMNFLCSFSLSSFRPKTLTYYLSIYGIADYFYNFAAYSSLDISSSNQNNSVTLQQKTVPYIESASSLETSSKILYLYGKGFKINDATFKVEIQMDTGTISVSPDITTGFILVLYDLEPSYQYKVKVLDQYSFSNTITIKGPVATPPPTTPPITPTCKSDCGVNQGYGKCVNGGCVCNPPRSGVDCSSIIDTTPVIIPDPVKPSVNVTIPGTGTSIEDPQFTSFVSVLALQELDPNENEKVVIGHVFNNDKWIIVDEGSFTNEQVSTVQYKYLIDNSLNTTIVSTVQVFAQAANITFGNQQLYMNPSTIKFTFNITSYPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWU2</accession>
    <name>A0A8J4UWU2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">UbiA prenyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_008888</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000594">
        <property type="protein sequence ID" value="KAF2069793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWU2"/>
    <dbReference type="OrthoDB" id="203513at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042371">
        <property type="term" value="P:vitamin K biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13962">
        <property type="entry name" value="PT_UbiA_UBIAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026046">
        <property type="entry name" value="UBIAD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929">
        <property type="entry name" value="1,4-DIHYDROXY-2-NAPHTHOATE OCTAPRENYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929:SF4">
        <property type="entry name" value="PRENYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2069793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="233" mass="27102" checksum="3A261BFE94A426B7" modified="2022-05-25" version="1">MNIIKTYLVACRSFISSTILNGFLMGTITSMVHSGSLNLTKSMIVCIICFQTHNFVYLINSYIDFLNQVDDKVTAVDRTLFDVITVNHLKGYLKFILISIISLLTLTFYWYGYKESLTFFYFVLFHLLCGFTYTQSKYVGLGYLPFIYYHVSVYWFFYYSHSGYLPLTGPPFYYSFLIAFGMGNCLSGNYVRDYEDDKKAGIITLATYFGKNRAIQIIKFFIFLFVSFINNIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWZ3</accession>
    <name>A0A8J4UWZ3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_008831</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000585">
        <property type="protein sequence ID" value="KAF2069853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWZ3"/>
    <dbReference type="OrthoDB" id="2384350at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000006">
        <property type="entry name" value="Breast cancer type 1 susceptibility protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031099">
        <property type="entry name" value="BRCA1-associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13763:SF0">
        <property type="entry name" value="BREAST CANCER TYPE 1 SUSCEPTIBILITY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13763">
        <property type="entry name" value="BREAST CANCER TYPE 1 SUSCEPTIBILITY PROTEIN BRCA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BRCT" evidence="10">
        <location>
            <begin position="974"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="10">
        <location>
            <begin position="1067"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="27"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="98"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="350"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="732"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="788"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="57"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="162"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="359"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="375"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="397"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="444"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="455"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="513"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="538"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="663"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="796"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="807"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="842"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="865"/>
            <end position="911"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2069853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1203" mass="131496" checksum="FE44825D8EF1D751" modified="2022-05-25" version="1">MDSAPNIQRSETSDLTSLDAMLDPLYFKNQHSSPSKSIGSSLDTSATETSAPTTDVNSNSNNSDNSNSNLEDNDNIYHSDDELLNLVPCLTSSKRKGTKNVRFNTEKIQVIEPSPPYSSQSSNGSNGNGHGSQKTPDTSLDAAQSQSQAYQLQDTEMTSSQDDQDEDEDDDDYEDDEITSFSLSTDNQLVFYGNDSNSNNNNNNRNEDYYQSISLPIWDAITSQDPKLLFRNGGEKVKRKRGLTIPNKIKTIPDVTVDDMMKVGLLKIGDALSYTIGGHSHMAILLREGYIEYGVHRIPSVHTWIIKVLSELTPNKKYHWFSPWDSIFVRGRSLNYIRSVFESKYKTVSNSSASGIKQPPAPFNGPQPLPPFVVAKSNKSPAKSQSSPSPSPPPSSPAKSQATPSPQPSQSSPPKTQAPPSSSPSKSQAASTPSPQSSQTNAPSQPPQSSPSPSSPAKSQAAPSSQPSQASPPKTQAPPSSQAASTPSPQSSQLSQPSQSSPPKTQAPPPSSPSKSQSSQSSQRSSPSSPLPPFIVTKSSPSKSSQQPSQPSQPSSQSSQSSQSSVSSQQSSNRSNSTPSKSSQQPSQSSPSPKSSQQSISSPLKRKQMDTEQEDMNDTTKYSKHSSQDTVHNTEDNESTLPLDENHKVLNISFGEESNNNNIDERINSLDHNGDNNNNNNGYSMDERVEPLEDPDKTQPMFTEDYHNVASLDNEKTIDLIKCTENAPSAAAVVNEEEENDKSTQYATIPAPSDFRDLSKEKFSSQDDQSTLSSKSSLPLVYVPVNTTTTTNDNNSLSVPFSKTPFSSPLNSQSPHSSQDYIQTTNNINTKTTSSSPVSSSLPITKTPTPQLNVSASQPLYQQIPSTPNSNSKNSTQNQSVPPSQSSSFVNYNHSHANNNNHISSINSSSIQQTSDHENSNINNNNNIIEFSNSQIPNLSFESMEDKNSHIDLSKNSKPVILGTRLTRLMQFHIISLTMALGGKYVTTWSEDVTHVVCSTLDDTKLSQRTIKYQLGISKGLWIVSFDWILESLNQNQWVNELEFEIQGDNVAAKGSPSKARQHLLFETKRLFYGYSFYLVGEFESPSKQELESVIKEAGGIIIKDNSLPPKPQNIKELLNTKSIVLCHPDFMFNNSSSGGVASSSNSIGIVNRVYLETKHHPISFKWIFDCLSHYEILSRENYIQGITTSTNNNISTQQSVSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UX83</accession>
    <name>A0A8J4UX83_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">V-type proton ATPase subunit F</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_008095</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the V-ATPase F subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000471">
        <property type="protein sequence ID" value="KAF2070590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UX83"/>
    <dbReference type="OrthoDB" id="10261947at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033180">
        <property type="term" value="C:proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10580:FF:000004">
        <property type="entry name" value="V-type proton ATPase subunit F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10580">
        <property type="entry name" value="ATPase, V1 complex, subunit F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008218">
        <property type="entry name" value="ATPase_V1-cplx_f_g_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005772">
        <property type="entry name" value="ATPase_V1-cplx_fsu_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036906">
        <property type="entry name" value="ATPase_V1_fsu_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01101">
        <property type="entry name" value="V_ATP_synt_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13861:SF2">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13861">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01990">
        <property type="entry name" value="ATP-synt_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015945">
        <property type="entry name" value="ATPase_V1_F_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159468">
        <property type="entry name" value="AtpF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR015945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2070590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="120" mass="13503" checksum="B8EF5293B6205789" modified="2022-05-25" version="1">MATKNITENALVAVIGDEDIVTGFLLAGVGQKDKKKNENFLVVDNKTSQSKIEQAFKSFTTRHDIAIIMISQKVADEIRYLIDEYNQVIPTILEIPSKDHPYDPTKDSIMMKVKRMTGSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXF2</accession>
    <name>A0A8J4UXF2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Alpha-centractin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_008278</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the actin family. ARP1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000496">
        <property type="protein sequence ID" value="KAF2070408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXF2"/>
    <dbReference type="OrthoDB" id="27221at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10216">
        <property type="entry name" value="ASKHA_NBD_Arp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000502">
        <property type="entry name" value="Actin-Related Proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000008">
        <property type="entry name" value="alpha-centractin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2070408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="382" mass="42961" checksum="49D07ED5C6CDA422" modified="2022-05-25" version="1">MSVEFSNQPVVIDNGSGLIKAGFAGQDPPSYIFSSLVGNPKYKKVMGLNLENDSTYFVGEKVHEWRGILKLKHPMEHGIVTNWENMERVWTHTYDLLKIQSSEHPVLLTDVPHNPRLHRERAAESFFETYNAPALYFSIPAVLSLYASGRTTGIVLDSGDGVTHTVPVFEGFALPHAITRIDIAGRDITDYLQFLLRRSGYNFKTSAEREVVRIIKEKSCYVAHDPQKEEEMLEPDSTSSKPVQTPYVLPDGNVIELGAERFRAPEILFHPDIIGDESLGIHQCLDSSIRKSDIDLRRTLYSNIILGGGSTLFQGLGDRLINEVRKLAPKDVKIKISAPPERKYSAWMGGSILASLSTFKDLWVTHQEYEEEGANILHRKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UY99</accession>
    <name>A0A8J4UY99_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007046</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000355">
        <property type="protein sequence ID" value="KAF2071638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="17315at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="485"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="485"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="531"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="555"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1510" mass="181371" checksum="722F6106A74FA4D3" modified="2022-05-25" version="1">MVRVNCDYIPAWPFLYYKKTTRTILHILWPLITWNESRSGNKVFALFYFLFRITYKERIDTQYFDFFWPIFHFEHNDKGFRFGIRPLMWIISTDSTFKFSLLGIINIFSKHNGDDVWVVIFPLFWYRKKNGNVFWTLAPIIWVRYKDSIDRLLVLVLPLGLFIRGTKGWLMNIAGLFHFSFHDDFTKVFLLPFFTYSNFHSVEKRLTFFVIVHVTWGPTKYRWIVFPIIFCTSKDSDNFFSLYICVVFFYRKRTNFKQLCFMGIYFYQRTMGYTVNIFLLLFFYLRAPNYKYLNILIFFNRHVNSECKRHIFFPFLWIKKKNDSHFIFHLWPLFGFKISQKDNGFAIYFIYPFIAYENYYGGSLKSFHVFFGFIHYCRDLNNSLYRHGITPLYFYRKTPKSVFGQILLYIWYKKVLANQFQMKKFDSSLENSTDIVINRVDETSNSYEKETEVSVDLEKVGYPGNFGNDSDQVQIEKGSIQVHLEKDSDPVHLEKGSDQVHLEKESDPIHLEKESDDTAGYPIKYDESGVPIPPPPPPMKAPGNDIGIDDDSDDIDRRKPSSDNEPKPSELDKDFEYGFKLYSLLPIFWYIKKDEFNSNRYIFFLPFFIMRWCKDIQSSYFLSLIYYQRKDSVTTRICPVFYQKQTLTRTLILSPIYIFWRREGNSFKLILPLFVDVHKSETHIMVLVFPIFVYYRKSIFKFVTLFPFFFRGQDASKGELFTYFFPIYGKSSKGKETNRYFLFPIFSFKRKPEKEFVSIDLFFPFFHYDRSGIDGSYSARILPFYWRSFNPVNEFLLIMPFFWKFVTDKDSRPQKNTLCLPFYFYRDSAEYEFTFATPALLPPYYIHYHREQSQVEQTYFYPFFAHKIKGNDHLRWILLILYRHTWKDFSDYMTMNILFYFKHRSEKWKISGVFPLWVQWHNNLENKYHLRVLFLVAYDRLMSATKAKTRLSFFWILLIKAGLFYRKVTKFAPPPSGDNPDASPEISQLGTKTYFLLLFHKKFNSYTDYSHFSLLWLFKSFISFIYVEKHGKDSLFYVFLLFFYKRTSSLSRTAVIWLFHPIASLFLRESTPFTIITRFFPLFWVRSTPDRVHNDDSIPGKASIQSRAVSILYIVPNFGLINYRRTQQKFALYFLPLFYYRKCKRDYSTIFSLFWFAVPHFSLFNYTREGEIVRHRVFPIYYRISSDCDHGHGTYINLLSIVWLGHPVVALYRFHKTHHNRVICFFPVFWSKYTYATEAFHLSILYIVRKFGFIDYWRNPDFTRFYILFFFFSCNEDNTRLYSLIYLVHPKVSFICYKGSTTKTKFRIALIFYFNNSTHDDIARCHNELAILYLGHPKVSFIGYWSRHTVAHFHIVLLLWVSKIESYTQVGIFWIGAAKSSLYNNPYVNQGIVKKVYSLWYPLSLFLYYDDGAYKTLHLMPIFKYSSQTQKELDKFWLLGGAVYFRSRGDVSEFRWFYRWIRVKSGNTEFVFEFNPFVAYKKKRGETKLLILGGMCGCYSSFCRVCCIEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYJ4</accession>
    <name>A0A8J4UYJ4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005795</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000240">
        <property type="protein sequence ID" value="KAF2072900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYJ4"/>
    <dbReference type="OrthoDB" id="18778at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="89" mass="9859" checksum="8D59F691DF02C9D1" modified="2022-05-25" version="1">MGFFSFLGSLIWFIIKTPFLLTWCLVKLVFHPISILVALIALYFYYTSLSAEKRDVPFVSKLAPSGFKPVSSTPINGTKGAKKLATGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYN0</accession>
    <name>A0A8J4UYN0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_005482</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000220">
        <property type="protein sequence ID" value="KAF2073200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYN0"/>
    <dbReference type="OrthoDB" id="20630at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0085020">
        <property type="term" value="P:protein K6-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF8">
        <property type="entry name" value="BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="44"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="77"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="110"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="196"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="401"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="271"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="324"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="324"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2073200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="448" mass="49217" checksum="5F572907D1A3C918" modified="2022-05-25" version="1">MGVSESKIREDAINCIHFGYGYKKFRKLVKKMKKMDINSIRDSSGSTLLNLSCQLGRKSIVFELLNLGANPNIPNNLGMTPLHHSMNNSDEESVSMLLKHGCNPNVVSETATTPLIIAVKKGDEKLVELLFGLNNNNNHSDTFGASASSFRSTSTPTIDGINITPIGSGGSSSNLNNNSSSSSNNNTIDVNLCDKQKMTALMWAATEGNDSLVKFLISVGADVNRRDNQGRTAMFIAKSGSHLNIVNMLNEQFCKVNKIILSKPSNNLLNSINNYNNNNNSSSSNNNNSNNNNNNNQQNVSHQSSVALPSQNFYTDSLASSWSSSDSNLNKNNNSSNNNNNNDIDKPQPSIQQIDNSNILITTNTLNSNNTPNISNKSQLKRDHSTLHSTISSSSKDERYCKVCWERPSDIVSLWCGHISLCMFCSQYLQTCPICVQTIERVQRVFVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZ49</accession>
    <name>A0A8J4UZ49_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Autophagy protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_004654</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000168">
        <property type="protein sequence ID" value="KAF2074040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZ49"/>
    <dbReference type="OrthoDB" id="20368at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043548">
        <property type="term" value="F:phosphatidylinositol 3-kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.40">
        <property type="entry name" value="Autophagy protein 6/Beclin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007243">
        <property type="entry name" value="Atg6/Beclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038274">
        <property type="entry name" value="Atg6/Beclin_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041691">
        <property type="entry name" value="Atg6/beclin_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040455">
        <property type="entry name" value="Atg6_BARA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12768">
        <property type="entry name" value="BECLIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12768:SF4">
        <property type="entry name" value="BECLIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04111">
        <property type="entry name" value="APG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17675">
        <property type="entry name" value="APG6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Atg6/beclin coiled-coil" evidence="5">
        <location>
            <begin position="449"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="Atg6 BARA" evidence="4">
        <location>
            <begin position="581"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="86"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="191"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="303"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="359"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="518"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="133"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="198"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="304"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="359"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2074040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="757" mass="85911" checksum="45BFA1C181537B3B" modified="2022-05-25" version="1">MDLFCQKCYKQLELDESLLEIDQTLFTNNDLSDLSGLIQEVEKLSYKITLNEKIKEQQHQQQQQIQQQQQIQQQQQQQQQQQQQQQQQQQQNPQMRKAKSGFFGRRKDINTSSTSVPTTPQPESSPLPSQYNTTTTTATSATQSIPLTGSSSITSSSTAIANNSEINIPTTPSSSSSSTFIQFYPSTSAGTSIPNTEGSGSYSSSPTFGSLSQHRLSSSLSKSISTSLPTAASNTTPSKPTTTTTTTTTTTTSNTAAVSTDPIKEEEDNNNTNNNSESSKKDNSNRNLSRQHSVKINLNEQNNHHHHHHHHHHNRQSHHPISSSPSKQRLKYLRSSSKSLSLASLPIVSQEEKELIEGNINKNNNNNNNSNQANSEITFSPNNNNNNNQIGHSQIIYNNQNTGTTNINNTLTTPTSPILNNQNQNQTYKHIQALSLFKCTTELIQLDIPLCLECTKLSISELDDENLVIEEEISVYQGYLKQLIEKESTFIESIKTIDQTISSLADEETYLYKFLKKIDSEKKTMDSLISDLHEKKEQLADIEQQYWSAYSELHYEAIRNQEEQDQVNVQTLWVNDHLENLKQTSILNDAFHLWHDGHFGTINSLRLGKLPSQPVEWSEINAAWGLSISLLDNMAKRLKYRFSQFILVPNGSSSKIEKKDDPNTIYELFGSSDISLGRLFWYRRFDNGMVAFLQCIKELCDFIKIQDPDFSVPYEIDKDKIGGMHIKMQFTNDDNWTKSLKYMLTNLKWLLIYIVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZX2</accession>
    <name>A0A8J4UZX2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7743 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010241</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000989">
        <property type="protein sequence ID" value="KAF2068432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZX2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035203343" description="DUF7743 domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="2">
        <location>
            <begin position="420"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="543" mass="60639" checksum="AFD9358C4D6215C9" modified="2022-05-25" version="1" fragment="single">MPNVLLFTFICTLLCFFTNSVYAIDNLVVEPVLPYGIEPYGLKLSALNAGSCIMKFKFRHTFIITDTLTCGFVATPTLSCAFHSTPNEYFTHTATVQLASDVLASNISLNIRFINSTEIQFPLVHLNSNPFSYNCVAPPAAPPTPTLSTVRFLNTSYSLSTSYFQSYLSLDSSFTRPLEPAYFTTSTAGLLVSSEFISPQSYLITFKYDPVFTSVQSNTITLVGGLTGFTISIPNPVTLNGYPQVQQKDLVVKSNSNPPFTGNYIFFTQNDPTLKVAPIFSLNYTPLNGQFPSKMNMYYKNKVIEAPRYITASQFTALGSPLVAPGLTTADLSLMVNLPMGTFSFKTYKSTTLFAQGDMRFSFYLDTLQLPVSYYYPLGLCYVDANMRLYEMSFSYSYIKSMSISITTFSKKDYSFISAVDPAVATLQSIQVLPLNETTSLLRLNISDSLGFYYAQVNTPSRVFYVRGWKNNLNGVYEAYVPFKQATSFNISLYNDGVNRIDYSNAQLYLNFSLPQPLPLISLFKKIKNVYFDKNTVSVSNSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZY7</accession>
    <name>A0A8J4UZY7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_005010</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000189">
        <property type="protein sequence ID" value="KAF2073693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="21216at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021429">
        <property type="entry name" value="Mediator_Med24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12898">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12898:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11277">
        <property type="entry name" value="Med24_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="234"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="318"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="600"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1285"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="259"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1285"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1315"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1368"/>
            <end position="1381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2073693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1448" mass="166545" checksum="33582555606012A9" modified="2022-05-25" version="1">MISSATPDNNTIAAAIAADTAVDSDRNNDVIVNNSRGSKNNTIDQQPQQQTTLKSLIVDSWRQRLSPFEWSLKINQSFHLNNTSNKLLKNDVESELCDILLKQMYIGKIPSPLLVSYLRYAISIKLVSYTEFFQSVFKYSSPSKPQHFSVILDIVLDIINIIVDSSSLSPATTINQSINNNQYYFQSTLRQDQVYSNSISKHIFTPTNNNNSNSNGKEQQQQQHYMNGVDILDEYSTPTTNTQKRKRKSSSTPPPPQPSTINNNNNNNNNNSRKKKTELDEQQEFQKHLKNSSYHKHLLSYTKLSTLLFNAIEHEFQQSNTNNNDNSTSSNNNNNSNNEKQPPIKSIFYENSIKSLEILNILLNNSKIKSCIYLSKYEFTLDDFKELLDSFLLVQKSIIENYSIDNSTMIKSNQQQQQQQQMEFDLSQQQTQQTQQTTQQIEKKQACQLYFVIQKKYKHMILEFKKILVTQKPLLPIHEISALAHKNTHSEHQLILAIQLILEEEINGNHFKCSNTPYMANVPLESISFNHNIPDCFYKLQTLQSIKSMSDFEFFYELVKTIFQKLSFTEPSSSEYKRTKLFLLFKIPQFYSIYYYQKGGSNSSSTTGTTTSTSSTTATNRSNNRPLMEHVLYQISKFPQISSSQESTLLSLIQILLKKDLVNANLLKNLFPSFSTDIESVDSEIQCPPSTNLSFENLNSGSGIHSESDILKIKELLESLMDSDSDPTHVGLMYELLNVVKYHLIYPFDYQDQLLFVLFNVLDSIDFIKNKILIMVMTFIFSDPMVIDCLDAHGYLLKLVLKLFDICELFGTITNDPDIHFYFTVPYNLLYHLLFNIYNVKNKPYNLDLLKSKLALYNSTSTPTHTIKWLCDMIKDQNINISNEVTQETFDFVKALLDSQDEGTEKLQSFALRKYSPWEMVQRLPTTLFVIFNSFEQNLIEKDKVINYCSLLFQSIPYSPISIFHFIITTQLHTQQQPNQQQQQQQQSSSTLSLVEDLASYLSNLNESLSIFRFTGGMKEDQMKEHLGNVKILIELFSPIFETLNFIMFPLLNRIFYGNGIQSVLFNRYSLSFISPPTELFYMGASVALHEIIEKFLGQTTPNFHNLPRDLKYVLSQINTYQAVNLVTKEILDILLISNQIDSPYSIRAMELGAFILGNCIGVESLPIFFNSIIPIWSENVSTAYQGQLLAYFSLCVVLFSDSLFYIDQQEQSIVKTFQIFLTLLKDTNMRMISYSPLNGLISFCITFVSLLIHLTDISSKTIHPDVLLAIQKLYHNDKFNLNHSSNSNSSNSNPMTSNNTMNSNDDDIDKNNDNPENNNVASNDNDSNNNNNNMSPILTDDESMDNPQSIEQKQQQEDNQQEDEQQKQQQQQLQQQKQQQPTKLSNIEKFKVSIDSKYMFSRSIFNQLDIHQLEDLAKSIIGLSKNYFLATSIFDKHDLQYCSNLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V047</accession>
    <name>A0A8J4V047_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Chloride channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_003570</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the chloride channel (TC 2.A.49) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000113">
        <property type="protein sequence ID" value="KAF2075135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V047"/>
    <dbReference type="OrthoDB" id="428525at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04591">
        <property type="entry name" value="CBS_pair_voltage-gated_CLC_euk_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03685">
        <property type="entry name" value="ClC_6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051280">
        <property type="entry name" value="Cl-channel/antiporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11689:SF60">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11689">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN CLC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0129">CBS domain</keyword>
    <keyword evidence="9 11" id="KW-0868">Chloride</keyword>
    <keyword evidence="6 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="255"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="291"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="339"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="382"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="458"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="483"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="517"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="12">
        <location>
            <begin position="712"/>
            <end position="759"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2075135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="759" mass="85573" checksum="477CC57FF02C35D1" modified="2022-05-25" version="1">MGSSLNKPLADKATDVDNQASMIYDPWRYREATNNEPNSKYTKSVTHRRRIHRLSPFEKQKMKNTQSLNFTVNDNLLLRAEAEKTTKGFHLKKTFGKWVICLFIGIILGCIAYIIKTTVGLLQGLKFHYTNHYISKGMQSYAFLTFLGINLLYVFLSSVMVIVAGPLSSSSGIPEVKGYLNGVKVPESLGFRSLFGKIVSLILSYSSGLFVGPEGPMIHIGSAVGAAISQFKSSTFNIYPNIFLPYRNDRDKRDFISVGAATGLSAAFGAPIGGVLFSIEEVSSFWSRQLTWRTFFCCMISTFTVNFLLQGIGSSPDIHDSGLLTFGFSRLYLYRYSELLCFCLLGVIGGLFGACFVFMNIHLNKWRKEKLKLNPFFRLGEALLVTIVTSILCYYASFIFGCRYQSNIIIEQSVCEDQTNTEMVQFFCPTGMYSELASLLFGNPDQALRRLYSRTQNMFTLPSLLVFTLFSFFLSIWSSGLWVAGGLFVPMMMVGAGFGRLFGQIVGLWFTNIDSSIYALVGSAAMMAGYCRMTICIVVIMVELTEGTQYLVPIILAVMIAKWVGDFFNESVYEHLMEQKYIPFLQTLPPHSTSNIRVTNVMSKLVSVLPEVCSVRLLLSILNSNYHHAFPVVNTGETGDSRLYRGLILRNDVLILLKNRVFYRGSGEEIYLDDNFDYEKFMQETSKKPPSLSDLNFDQFELDSFIDLRPYMNSSGITIHNTFSFVEAYKLFRTMGLRHLPVIDINNEVVGIITRKDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0H3</accession>
    <name>A0A8J4V0H3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Geranylgeranyl transferase type-1 subunit beta</fullName>
            <ecNumber evidence="4">2.5.1.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Geranylgeranyl transferase type I subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_009469</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein prenyltransferase subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.59" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000717">
        <property type="protein sequence ID" value="KAF2069212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0H3"/>
    <dbReference type="OrthoDB" id="24893at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005953">
        <property type="term" value="C:CAAX-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004662">
        <property type="term" value="F:CAAX-protein geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02895">
        <property type="entry name" value="GGTase-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041960">
        <property type="entry name" value="GGTase_I_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF4">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE-1 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="13">
        <location>
            <begin position="9"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2069212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="358" mass="40237" checksum="595EDADC367A3DA9" modified="2022-05-25" version="1">MTDIDYKGVTKFFERSLNTLPKPYQSGLSNHLSLTYFTVSGLDLLKRQDIIEKEKQNIIDFIYSRQILPSKTNPDVNREYCGFRGANFVGQPYCVNKSHFSVDGETPSTYDFPNLANTYCALMILRIAGDDFSSVNKKAIIDALKCRSQENGSFNSAPKSTESDVRFIFMACAISYMLNDWSAVDIEKTTQFIMDSLSYEFAFGQSPEQEAHGGSTYCAVNSLALMKKLHLILPFKDKFIRWLVNKQITGFCGRTNKDPDTCYSFWIGASLDAISSFNLIDFNLMKMFIQSAQHQVIGGIAKEPSNLPDILHTYMSLSALAIGGDENLMPLNPVLGITQRAAGKEWNDDLLNQLLKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0P8</accession>
    <name>A0A8J4V0P8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_004048</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat PAAF1/RPN14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000136">
        <property type="protein sequence ID" value="KAF2074653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0P8"/>
    <dbReference type="OrthoDB" id="27537at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051179">
        <property type="entry name" value="WD_repeat_multifunction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857:SF19">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT RPN14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857">
        <property type="entry name" value="MITOCHONDRIAL DIVISION PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="146"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="188"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2074653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="420" mass="46473" checksum="F51C5C160DB8284D" modified="2022-05-25" version="1">MTSNSKLVTTSREIPNHFLESIKIQYDWELAYKQDTDNFWVSYQKELKNIVHGSIRSTRQSNNKFLFESDQGFKLDIQENLNHIKIGCEKYTTNIYSPYQVHKVLNGKKRIMSMGVSSMGELGVYGSTDGILEVFEISDGQVRRKLDGHAGDVDFSMFFPSGTVILSGAGDGRLKIWDALDGSCAQTLVGHTGGIISASLVDRGRNIVSASRDGTSKLWDVPSASVIHTLCKLSRPVNDCFVASSLLESTNNTGEKTDEREVGTQGKTVVLAAEEGFLQIYDLRSKNMVSQLDIPLLSEQTRPVAFNACHVHNSLIVGGDHVGNIYLWDKRNLSTPFSRLQFSNSPIHHIKSNTSAKSSNSFWVTSGDGSVFLLDLDKNSITTSLSGIDTDVVTSFNVVKDMAFTTSRDSIIRCYNKLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0R1</accession>
    <name>A0A8J4V0R1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_004012</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000134">
        <property type="protein sequence ID" value="KAF2074698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0R1"/>
    <dbReference type="OrthoDB" id="20402at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01093">
        <property type="entry name" value="CRIB_PAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06614">
        <property type="entry name" value="STKc_PAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000802">
        <property type="entry name" value="Serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033923">
        <property type="entry name" value="PAK_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF22">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SAMKA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRIB" evidence="15">
        <location>
            <begin position="705"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="802"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="75"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="319"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="360"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="75"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="406"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="471"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="515"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="542"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="559"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="575"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="596"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="624"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="645"/>
            <end position="665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2074698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1088" mass="122510" checksum="2B1E0B2A8788BF61" modified="2022-05-25" version="1">MDKSANSNNVSSLRQKFENFLARNTTTTHRKSAPGGISNEQIKNHNNKFLSPVKKVEQSRNSFNYSSSQQSRSAFVSSSGLGSSNSTSCNNSSFSARNSSSGGNNRSSSGSSKSTISVSQQQQQQALPQVLPKSPSISSEEEHSYSDFTSHLEMQAATVTASDLKREREQYFKNKEEFTLEDDDFNQDFDFKNSSWADKRERLIRILLKHKFTITEETIREELNKIRDSEVDKIRFEERKRPSVNVEELKQLEIEKLRQRLHVQEFELFKQEEINKIRKEERQKIEKEYSNKSLLSTQEIDQLKNDEYKATSAINSNVVISDTNSPPHPSTYQQPPQQDDVDSDEEMERLEQLKRQREIERLREEEEENEDRLERELSQRRREEEERIKREEEEEEEEQRNHLRRLKELERLKQLEEEEEEEEEMKKSSRASESSSTTLSSLSSTATSNSTFRESYDQRKTPTNLLNLIASKDSPSSSVSKQQHSNLLPPTSPSTSTQLPLVDSRSRSHTMAAASPQISSSQDSNQQSQGQQGETKYSRSHSGGSLSGLSLPSAPPIPAGGNSKPSSLASSSTSTPNVTLSSPQVVGGSSANSTVPSSPISASNSISSPSLMASPRNEEKGRKNSVSTSNPSHENGKDLNSSGGKEGKGKKSSKDKEGEEKDKKSSFFNKLFSGGKSREHRNNSGSSSGSSNDDDKKKKKLSPRVGVPFNVKHDVHVNFNADTGFEGLPKEWEVLIKSNFQEPEVMQHPEEVLNVVKFHTQYNNMSSAPPSHIQQPSIPPPSDEAPVTLNDLISLDDPKKIYYNINKIGEGGAGEVFEAVNSRNNQTIAIKKMKLKAQNLKTVINEIGMMKNSLHDNIVQYIDSYIVADELWVAMELMSGGCLTEVLDQHRDIQLTEAQISYVCAQVLHGLEYIHRFNRIHRDIKSDNILIGGKGEIKLADFGYAAQLTQARQERNSVVGTPYWMAPELIRGNNYDFKVDVWSLGIMTREMAEGEPPYLEFPPLRALFLLTTQGIPPLKDAYKWSKEFNDFLAQCLEKDTDKRPTAATLLNHPFLKKACSGLEFLKAVEASRTIKENQLQQFNSTYNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V180</accession>
    <name>A0A8J4V180_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_001877</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000048">
        <property type="protein sequence ID" value="KAF2076800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V180"/>
    <dbReference type="OrthoDB" id="8815311at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00173">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033646">
        <property type="entry name" value="CLU-central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025697">
        <property type="entry name" value="CLU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027523">
        <property type="entry name" value="CLU_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT EIF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601:SF13">
        <property type="entry name" value="SH2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13236">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12807">
        <property type="entry name" value="eIF3_p135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51823">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="Clu" evidence="5">
        <location>
            <begin position="434"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="4">
        <location>
            <begin position="1046"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="224"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1103"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="14"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1143"/>
            <end position="1168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2076800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1197" mass="131868" checksum="B44DC7497CF4D8AC" modified="2022-05-25" version="1">MARVEPNSKLTHISGSSNSSSGISSSNSSSSNSNSNSNSNSSSSSSKISRSSSKEERLSSGGKSSKSNPTKTTISTETLDLIVSYLISLQEYHQKFENNSEDLDNFINYVNRLLYLKRKIISLIDITSGDGVLTDSKGRKVSINELFIYIDKLKKSQHIASDLFEISCNNPVLVPMGPINRVDHYNQAPIIVPKFNQAVYYNCGSSSSKPSSLSSSLSASPTMAPKTLASSTSTTSLSSLTSSTSTTTNLTSSSVSINTNLTSSSSSLCSSASSIYGNCPTPPISSSSNSINNSSSGNDLLKNSLNSSSNNLNNSSSSSSNNIIPIPIPIQLDGSNRFELNGVSTLSSSTNISNVPISSSPPLSVSPFNNSLFNNNNQVKSFKNNNTSNSNSSAFKQLELSPVYVDDGYTPPISTLSFEKLSLDDFETTTTLNTTPINDLLDNSNNIKQQEPNTEPVRNWSLDFWNALQMDHNCGNESLIRSQRIQQIAEEFVSVATKHGMTIIQEKGKSFGWTIPPYDVGGVAGGLKYLKDGIFFKFAVDVYGIYGGNAFAMKSAGHELKGLMGYFNCQIDGLYLPLMALIDYMGYRLIATSQLAIDGDNTLVYGSNDGGDTVHNKSQAMDGLMKQASEILNLKGHYTGQGENKVFLYGPCDIEGHITKDNHYVIIDTARVFPPEKPIPGKIGSHLYNLLRPELVRSNKLPLSSDAFTKFSGPDSEQNNEDVERSQKRLFVSIIPNFVLSLNQKVRRSKCENILELSHMLMLVDSMHREGINVRYLNRLKKTDIIAIDHRLSAILNTEIIARKIKNRLREFLRNETSINKEKIAQFLTRMVIISLLQSCDLDGFSLELLLHRLQQMTGFVFRDGLARSLETSHIMHESDIISITPKVKFLHMVPYQQGVALFHQAISASKLGTTDEGGLDDSTSRADKIFQQASDKFKQAIQSKPDDYEILTHWGILFFMRAKLLSQTKDYIQEHGSAEIEKLYETAIEKFQQAVKIKRNHHYAIFNWAEALKNWSAIKPAVEANSLEKLSKLKLQEAGVFEKKWFFGTLETNGAASLLSGKEAGSFIIRNSNSRPGSFVFSYLSDPHRKIQHSIIKSSSHGYHVENLPPRKVPSSPNSSGHHRSGSRTGSSSKDKSSSKNEAIQFNEAGTTIPTRNSTSLSSQTPVTTVSYKTLEEFVSDKIKQGYIPITKGWDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1B9</accession>
    <name>A0A8J4V1B9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_001758</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000044">
        <property type="protein sequence ID" value="KAF2076920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1B9"/>
    <dbReference type="OrthoDB" id="28773at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16628">
        <property type="entry name" value="RING-HC_RBR_RNF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22249">
        <property type="entry name" value="UDM1_RNF168_RNF169-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000137">
        <property type="entry name" value="RanBP-type and C3HC4-type zinc finger-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031128">
        <property type="entry name" value="RNF14_RING-HC_Zfn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="293"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="297"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="139"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="181"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="140"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2076920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="503" mass="58533" checksum="CE112DECE9311A32" modified="2022-05-25" version="1">MIKDYEDFTLLGEKQDIVIGSEEEEQLVNNSNNNNNNNSIRSNSDVEQKLQWLETIASVLPSVQLSVALKVINNFKDIDSIIQHLLTEESNSDNTTPLSNSVDINDNCCHNNNNSNNNSNHVHHMIKETPLTVVDLPFINNNNNNNNNNNSPKTTTTTTTTTTTTTTSTASNSNDNKDDYYDQEQQRIDEEQKQILELLNKEMEEERIQAERLKNQNEQSMQYILSLFKQEKELQELNVNQSEQSSQFIQELLEKEQREIERKRKEDEEMNERMLKKVLEEEKKLKQEELENKTYLCEICFSDCKIEEIYILEECHHRFCKECMGMHFKTNIVNGNVRDIRCPNIQCNRQVTYQEIKHNVSRAVLAKYEDFLLKASLKEDPNSRFCPRPGCSNAMIGNPSIPMIVCTSETCKYAFCFNCLDEWHADSTCENYQQWKKDNNSGSTLFITWANKNTKECPKCKSRIEKNGGCNHITCKCKHQFCWICLENYTSAHWSTSKCKQYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1I5</accession>
    <name>A0A8J4V1I5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_008198</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000485">
        <property type="protein sequence ID" value="KAF2070487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1I5"/>
    <dbReference type="OrthoDB" id="10261027at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09487">
        <property type="entry name" value="SAM_superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000034">
        <property type="entry name" value="Kinase suppressor of Ras 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001060">
        <property type="entry name" value="Probable serine/threonine-protein kinase DDB_G0278535"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000433">
        <property type="entry name" value="Probable serine/threonine-protein kinase DDB_G0278535"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF288">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="151"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="222"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="257"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="14">
        <location>
            <begin position="314"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="442"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="379"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="719"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="10"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="398"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="725"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="469"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2070487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="758" mass="84978" checksum="0CBA0E1E42EA6F73" modified="2022-05-25" version="1">MSTNNFNKHNTSASNITTSTSSIGSIGNNTNPNISRSNIAAPPVSSSMEKKSGWGDLISRRSSTNLPAPPTSISSSSKLNKLHQSILAKDLGKVKQILTSKAKAKQYFTQFDQMDQTPLCAALKQNTYDIVKEIIHFYQTNKMDINEQDKNGYTPLHVAASNCDDQILVLLLNYDGINVNITNEDKNSPLHYFCQKFKSPNCQEPFQLFLKKGVDVNAQNKNGETPLHKSIMNSSVRLLMVNLLLEAGGEVNVLNYRGESPLHFAVYLGREDLVSVLVKAGADITIKGGSEKKTCYELALSSQPRNNKVINFLKNVQDIYNWLKSIDLETFWLNFVKEEIFMDLLPDIDDKTLLDSVGITSSGNRMKIIKQCRLLKEQLANQPPPPPLTTTTTTTSTGKSNITGDSFKSTPNSMDSTGSISSDDLKISLTNLEHWVIDPNEVEYTLKLGSGSSGKVYKGLYKGKEVAVKVLRAITTQSQLDEFKKEFQIMGSIRSQFMVTFYGACIEPKLCMVMEYCSKDSLYHVMNTKSYDIGWERFFQFTMQMTLGVQCLHNWNPQIVHRDFKSLNLLVNEDWECKVSDFGLSRFNTADNLETLSKIRGTFAYCSPEIANGTGCPYTTKSDVYSIGIVFWELIIRVLTGEYSRPYSEYSHIKMDFQIMLNSKEGLRPTLPQCCPVGLSNLYYQCVHQDAAQRPSCEEIIETLNRLRHEYMSNKSHWDSLIKKPTPTSSPPINSSSSSRNNSSLSTTPTPTSPPTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1X2</accession>
    <name>A0A8J4V1X2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Chloride channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_001088</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the chloride channel (TC 2.A.49) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000024">
        <property type="protein sequence ID" value="KAF2077625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1X2"/>
    <dbReference type="OrthoDB" id="4564at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050970">
        <property type="entry name" value="Cl_channel_volt-gated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720:SF10">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0129">CBS domain</keyword>
    <keyword evidence="8 10" id="KW-0868">Chloride</keyword>
    <keyword evidence="6 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="163"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="207"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="256"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="314"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="350"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="388"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="435"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="546"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="577"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="12">
        <location>
            <begin position="631"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="723"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="746"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="777"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="826"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="856"/>
            <end position="878"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2077625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="965" mass="107452" checksum="25E5B1B6B587612C" modified="2022-05-25" version="1">MILNNNSNNHDDDDEENIQQSGGDNNHESSTSFNPINTIRNINFNPLGLDLNPLNVDFQGHRDRVVNFFQHGTDDLNTRMQSVVGKLRSNEYRKKMTTVLHKIKDTKEYRSTNNTDYDVIFESVDMYSNLQADPTLDKDEIDVVTDLSSTKKRLHHWLKKERISTLMFIPILGIAIACMGLLCDYVLKELGELRTIIMPEEHSMYSWLNGVVFVGYSVVLAAISACCISFISPYAVGSGIPEMKSILSGINLSRVLGGKTLVSKIIGMVSATAAGLTIGRTGPFMHASAIIAQQMMSLKIFQNIRKNQIVRYQMFICALASGVVANFGAPIGGLLFSIEITATNCIFGNLWKGFLCATTTAIIFYLSRPWMGGAAFASVYQFDYTIKSFGWLEIANFVGIGIICGIIGAVFVFLYEKIVRFRLRYQAIKQSRVGLVVFIALVSATITYLAGPLVRKPLSYAMKEFIHDDEIEPFLFNVDKDNKNDPHRLYNIMVFIAVKLFLTAINIVLPIPGGAITPFIVTGAAMGRLFGELLHYHLNLTSIKACGYAVIGAAGLVSGTTRALSPSIFVLELTGQLSLLIPVLVCSITATAVGNFFNRPLFDIALKIQGLPFLSSYRSEKVYTMTAKQVMKKDINYLSMSSTVNEIKDFLEKFKHTFIPIVDSKQNMLLVGIVERSSMYILIDEHIERMDKMFEDYKNSIQNNNSVNNSSSNSNNQAIVEQLQQQQQQYNTADSIITDPKHDQQDEQDDDEDDDNARLIPSNNNSSNNSDFEMVNSPSSKGSSNSELVREIENENNSNNSSDKPHLEEINLEDEEDNSNHNADITVEDDDDDQEGVPLVNLNKDGGLRNRKSKNDSTTTNNNTTTTTTTSNDNNNNSNNFIISFEEIKNLKSEHIWGQHILGDIIQNGETAILMDLAPSQIPDLTPLNKVFHLFTMLGLGFTYVTSLGKLVGVITKTDLISKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2J7</accession>
    <name>A0A8J4V2J7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007083</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000358">
        <property type="protein sequence ID" value="KAF2071612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2J7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="28" mass="2775" checksum="DEC5B122E062AB4E" modified="2022-05-25" version="1" fragment="single">AVGVIKGVDKKQPGKAGDKGKAAPAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2Z7</accession>
    <name>A0A8J4V2Z7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_006591</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000311">
        <property type="protein sequence ID" value="KAF2072092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2Z7"/>
    <dbReference type="OrthoDB" id="20092at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000016">
        <property type="entry name" value="TBC1 domain family member 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="92"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="30"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="406"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="469"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="562"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="704"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="761"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="822"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="343"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="48"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="418"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="526"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="567"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="713"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="832"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="854"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="867"/>
            <end position="878"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2072092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="929" mass="106048" checksum="4C95D03103D81822" modified="2022-05-25" version="1">MDDLNTSLDITSPTKRNAKLDEHGFVVIEDDESTLDNNNNNSNNNNDKLVEETPKEAGKRLSREQKWIHIANNWNDYMINQPEKLLNLSAKGIPTNLRPIIWKKMLNINFYKNQYPEDYFIELCKKSENEHTEQIDLDIPRTFPNNKRFFTKRSKKDLLNVLQSYSYHNSKVGYCQGMSYIAGVLLMFLSVEDSFWALVALLERETMGYYIPGMPQLISDSILFQKILEIENQSLGIHLTKNGMDPLLYVTPWWMCFFTTLPEWGYVMRMWDVVLFEGINSLFRISIVILKYSAGYLFKKKGADGLLPYLLRPPLDEIGGIDFLLKMALELPIKDLVHQAKLRIEQEREEKLIAQKLKEQEKELIREQEELAKQQEMILQQQQAKKKQLGESGVFDRIIKTFIEDDSSGSSNEANGIKLTTKSSTTPSSPPPTPLKTPVKAQSSSFLSKRLHDLSNRIKSRFAPSNGYQPCNVIHESKKPRKSVARRPSMAPRNSIAPRSTTMAPRNSIAPRARKSVLPKQINNETSNTKTTTTTTTTTTTSTNDIQDNNDSKPITKIEKSIEKDSNNNNNNNSNNNENILVKKPRQSFVTRRSILGRKSVLARKSVSNRQRYLIRNNNNNFVNPVFEPVLNDPLSIKEEEEEIKPNPFKKSTFDSAPPSSPIPMIKPPPPSSTPLLKIISPCNISLSSSQSFNQHYNHHINHNSSMNDSMLSNHTNNNNTSIRSSIGGSPLRKSIYKGSPLKTPPRSITKSVELMKEIMKTPPPPIPFNTTSPIKKSLKSGSKGNAAWVTHQMFENFSLHSPQQQSSPTVDEKAFLREFSTPTPLKDFNNRQHITGATPTTPMSTNPKKRLLFNNNGTENQTPNLKSNSKTSQSQQQHNKKIKQQKPIEFSLESAIQEYNVEDQTELKEMSHQNYLFKKERKQWDLRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V302</accession>
    <name>A0A8J4V302_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glycerophosphodiester phosphodiesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_002335</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000063">
        <property type="protein sequence ID" value="KAF2076378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="197419at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001070">
        <property type="term" value="F:starch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08572">
        <property type="entry name" value="GDPD_GDE5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14447">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057506">
        <property type="entry name" value="C2_GPCPD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002044">
        <property type="entry name" value="CBM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051578">
        <property type="entry name" value="GDPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22958">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22958:SF39">
        <property type="entry name" value="GP-PDE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25329">
        <property type="entry name" value="C2_GDE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00686">
        <property type="entry name" value="CBM_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM01065">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51166">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SPX" evidence="7">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="435"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="468"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="502"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="536"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="608"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="domain" description="CBM20" evidence="6">
        <location>
            <begin position="768"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="8">
        <location>
            <begin position="1070"/>
            <end position="1443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="220"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="673"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="738"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1158"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1234"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1443"/>
            <end position="1462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="228"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="686"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="743"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="756"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1174"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1234"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1444"/>
            <end position="1462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2076378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1462" mass="162922" checksum="3E6BD735F30D74FD" modified="2022-05-25" version="1">MKFSKQLKNVSIWNHHYIPFQKLKRKLRNIFSPNVSPQTSLPSFGQGDGSATPSPHSGSTGMSISGDFLVLLEKEIEKANRFIIEEKLNLIQTRFEDLKTKYKSIDISSPFAVYLRVTLLNSATTASALQQQQLESLDLDQSSSSLGFFSEQQQQLNGYLEVDSITAFQIAFEDIIKQLAEIDHFSQLNIQAIQKLLNTYMKKKVKYSLQDQKRRRVSLTETTIPKPNTNTSGSNTSNTLQVPQSTNISNTNTSNDHINIKQEIDKFFNDKVSKLEIYTGTLVKKLTLETELEYQSICQSNLYLLNNSFIDILSNQEKELSKKVTLFCQNVVSNNIQLVNTTIQEIKELMKSLNIKDDQVMWRETIHSCIHKSCYLGNTDMIDLLFSSFSDIVDIHYCDEKNKQFIHIASENGKVDVVTLLLDKWGANIECNDFLSRKPLHLSTKGGHHGCVQLLLSKGALVNPIDREGCTPLYLACRQQKPNALVIELLLKYGALFTVGPYGRHPLHEAASRGNPENIAILFKLSSMDANVVDNFGRTPLFDAIRHGRLESISLLLTNGAKVSVFDEEKRTPLHESAYHGQKLAMEILFDSLNQVDDKDLINSQDDDGWTPLHDAAYLNYTGCVRLLLENGSNPFILDKGEWTPIVHSTYRGNIQTSIIIIEYLKTHPELNHESLPSTPRSLGVSANAATTTTPPSSISPPFLSTNPLSLSGSSRIQINNYLNTSSNINIPSTIQEVVSPTSSGNSGSLGNSSNTDKGSSPSNSKKILKSLMSKVTFRITSNNIKPGYVIGVVGNRKAIGSWNPSSALIFKSLSTSASGTNTNTTAATGTEESIWIGKVSVPVNVQLEYKYIVFDGSRLDTWEALPENRKFTPQEEEEFVDDGVFGKISIDQEQEEQQQEQQQQQLFTERGWLVQDTQLRIRFGETSATDPQRVTIQPVQLYNGKEQAGKIITTLRDRFSNIIPNSSSFSVSLPINKDQSVFFQTNQFHLAHVVQFDIYRRGMSSVLIGRATAFVSEMVSDNPPYKKKIPIINSSLVNIGELLFFPLVISPFTHPRISDVFSKTYWKSTLLIGHRGGGAENARNVGKYKRTHIKENTILSFVTAASLGAQYIEFDVQLSKDFVPVIYHDFEILSQNNIKMPINKLTLEQFKNFHLSANDDTDQHDKKNGNGNGHKAGINGNSSNASSAKNKVSLSRRGRSKSMQDIFNDNDLLNSATGVDDLLNQLNQQKSIQMTTPATSIQSSPPLESHHHGHHGGGRGGNPISDSMTTLEETFKNVPIQCGFNIEIKYPSQEKEQELSLHTLDRNRYVDIILKVVFEHAGDRSVMFSSFDPDICIICSLKQPKYPVFFLNNAGLTQHSDPRLNSISEAIRFSKSAHLLGIVTNSKILVEAPPIIGQVKSAGLMLCSWGAENNDPALVDLQETLGCDAVIVDHVAYVSKHYNSNTNNNNNNNNTSSTNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3I4</accession>
    <name>A0A8J4V3I4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein-serine/threonine phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_001871</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000047">
        <property type="protein sequence ID" value="KAF2076843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3I4"/>
    <dbReference type="OrthoDB" id="10252009at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008579">
        <property type="term" value="F:JUN kinase phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46377">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46377:SF1">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="3">
        <location>
            <begin position="86"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="4">
        <location>
            <begin position="156"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2076843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="27421" checksum="51FA23D5613F63AC" modified="2022-05-25" version="1">MNKLLEDINKKKKNMNHVNDTIVTSVDGKKYKVDLSGHDHDLKNNELIVPSSSNRGDISYGFVVDTKPDDKVYQVIIPNHWLSSGSISGNNSYCKLYIGSQDAAANLNELQLNNIKSILNVGYGIPNHFERTNSHSNSNHNNNDYVISYLNIDMYDDVTYNIRDQFNSCFEFIEKQTSILIHCNAGISRSSTILLAYMMNKFKKPLQECLSLVRESRSCVKPNQGFLKQLEEYEKTLKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V409</accession>
    <name>A0A8J4V409_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine hydroxymethyltransferase</fullName>
            <ecNumber evidence="9">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_005581</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the SHMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="9"/>
    <dbReference type="EMBL" id="AJWJ01000226">
        <property type="protein sequence ID" value="KAF2073112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V409"/>
    <dbReference type="OrthoDB" id="10265628at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000050">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF35">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7 8 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="10">
        <location>
            <begin position="11"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="8">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2073112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="457" mass="50625" checksum="0A0834340E260E95" modified="2022-05-25" version="1">MGSKAGNTPLVESDKEIFDLCNKEKNRQFEGLELIASENFTSRAVMEALGSHFTNKYAEGYPGARYYGGSEVVDELEKLCQKRALAAFHLEDSKWGCNVQPYSGSPANFAVYTALLKPHDRIMGLDLPSGGHLTHGYQTEKKKISASSIYFESMPYQIGADGYINYQRLEENALLFKPKLIIAGASAYPREWDYKRMRQICDRVGAYFMCDMAHYSGLVASKLLDSPFEYCDVVTTTTHKTLRGPRSGLIFFRKGKKVDVATGKEEDYDFESRINFAVFPSLQGGPHENVIAGVAVALKEAHSQDFVDYSVQVQKNAKAIGAALVSKGYKLVTDGTDNHLILWDLRPKDLTGNKVEKACDYANITVNKNAVYGDTSALAPGGIRIGSPALTSRGLVEADFEKVADFLDRIVKVCIAIQAKVGKKMVDFQVELAKSEELQTIKKEVEHFSKQFPLPGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V422</accession>
    <name>A0A8J4V422_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_001528</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. PYROXD1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000037">
        <property type="protein sequence ID" value="KAF2077143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V422"/>
    <dbReference type="OrthoDB" id="202203at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050260">
        <property type="entry name" value="FAD-bd_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429:SF2">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="7">
        <location>
            <begin position="5"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="7">
        <location>
            <begin position="275"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="199"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="205"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2077143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="509" mass="57026" checksum="72E3C947C430BE8A" modified="2022-05-25" version="1">MTSNHLVIIGGGIAGITCAESYCFVKPNDRVTILSCSPILKTVTNVQKISKVLESFEVFESKFTDIEFSHPNIKVTVCDVDSIDSKNRVVKTDQGDIKYDFLAICSGAKPNLIIKDSPFVIGIRDTDSIIDLKNRLNGAKRIVIVGNGGIALELVHEIKDCEKIWSIKDSHIGNAFFDREASQFLLHSKQPEQEEEGDNIIFENGNPNHHTLSNLSTPSKYKNEKGSALGPQWYSKYNFNTQEQQQQDHAEKTVIEYNTQLDTIYSERPPSVDLVDGDTKQWPVYIKLSNGRIYGCDFIVSATGVIPNSVILTKNHAEVAVSEDGGLVVDENMKTSIDRIYACGDVCFVPWKKSDLWFQMKLWSQARTMGRYTAQCIAKQSAVQAGKSADEIDEICSLFDFELFAHATKFFGFKVVLLGLYNGQTLGIDPSDDSVNENNDKGIKIYKRIIIGQQYVRIILEHGKLKGALLIGDTDLEETFENLILNQIDLSNYGEELLDPDIDIEDYFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V481</accession>
    <name>A0A8J4V481_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methylated-DNA--protein-cysteine methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.63</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">6-O-methylguanine-DNA methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">O-6-methylguanine-DNA-alkyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_001374</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a thymidine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:53428"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:13555"/>
            <dbReference type="Rhea" id="RHEA-COMP:13556"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:137386"/>
            <dbReference type="ChEBI" id="CHEBI:137387"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] = S-methyl-L-cysteinyl-[protein] + a 2'-deoxyguanosine in DNA</text>
            <dbReference type="Rhea" id="RHEA:24000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:11367"/>
            <dbReference type="Rhea" id="RHEA-COMP:11368"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:85445"/>
            <dbReference type="ChEBI" id="CHEBI:85448"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MGMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.63" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000032">
        <property type="protein sequence ID" value="KAF2077308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V481"/>
    <dbReference type="OrthoDB" id="1907495at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003908">
        <property type="term" value="F:methylated-DNA-[protein]-cysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06445">
        <property type="entry name" value="ATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000214">
        <property type="entry name" value="Methylated-DNA--protein-cysteine methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001497">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014048">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036217">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNAb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036631">
        <property type="entry name" value="MGMT_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00589">
        <property type="entry name" value="ogt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10815">
        <property type="entry name" value="METHYLATED-DNA--PROTEIN-CYSTEINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10815:SF13">
        <property type="entry name" value="METHYLATED-DNA--PROTEIN-CYSTEINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01035">
        <property type="entry name" value="DNA_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53155">
        <property type="entry name" value="Methylated DNA-protein cysteine methyltransferase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46767">
        <property type="entry name" value="Methylated DNA-protein cysteine methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00374">
        <property type="entry name" value="MGMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methylated-DNA-[protein]-cysteine S-methyltransferase DNA binding" evidence="13">
        <location>
            <begin position="106"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="24"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2077308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="193" mass="22426" checksum="5193ED07B1EBD473" modified="2022-05-25" version="1">MTTKRKLNTHTTNQSKKNKKEIEQDQEQLEEGEDENQYWDRLETPIGVLGFYASKDLLENVIVEDKSTAEPKPNTITNQFKDELNLYFKGKLIDFKTPFNYEQGTEFQQMVWRELMKIPYGQTQSYKQIATSLNKKNYSRAVASACRLNKFSIIVPCHRVVSSSGKVIGYFGKNGIDKQHQLYALEQKSLKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4D0</accession>
    <name>A0A8J4V4D0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mediator of RNA polymerase II transcription subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Mediator complex subunit 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_008080</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the Mediator complex subunit 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000469">
        <property type="protein sequence ID" value="KAF2070604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4D0"/>
    <dbReference type="OrthoDB" id="2281547at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051999">
        <property type="entry name" value="Mediator_complex_subunit_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019680">
        <property type="entry name" value="Mediator_Med1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12881">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12881:SF10">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10744">
        <property type="entry name" value="Med1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0010">Activator</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit Med1" evidence="8">
        <location>
            <begin position="380"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2070604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="620" mass="71003" checksum="A667D2263F858AEE" modified="2022-05-25" version="1">MTDLTRDITIHQTLDSVSSQFNHLFNDVGVLGSIDNQNFQSQLFHPLGTHNIDERYLSIDSNLRLIKLTCMQYRQWDSEKDFVKFQSTLKQSYTTQQKVNLIKKSLLDCSLVGKLHLDQLVNNNSNNSSSNNNNDQDNIIKISERKRKLSLLLSTFQEMTHELEKKYGTFSDLEELNSCIKNESEILSPESVFNCNISSSTFSLDIYIYGTGEIKEVKLVHISISSSGEDQGLDHPEFNEFLTQALKKDIKLFLQKVKNICNLDLLFRKFKQFDLQKAFSIFQYDFLALSEKLKEKYGDDNLDKLQNESIGLIKLDCCGVIIEFFHPLIDKLSKKRQPFSASIEMEIGVENSNISLNRLFNGATFEDTNINQNYFSNEITNFISSPVRLVFNLSAPLIISKEILMIILNNINSFNSNSNNISNLQISTSKKSISDKFSIQNHLISTLNNNSKDQSFDNNVLGTKQRYFYSGEQHTGYEISRIPFNHPNQILPIIQLLRQQIVWNLLFTSCFQSLDNSSGNSNNNSFNNFESPPIFEISSNPPYSISIIFLHPNEQTFHSIDIEITNNGLINATYYGTNDDDTPTEIQNKSNYLCSLLTKSLSIPLSFYFLLKEDADQMTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4F0</accession>
    <name>A0A8J4V4F0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Leucine-rich repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000202">
        <property type="protein sequence ID" value="KAF2073472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4F0"/>
    <dbReference type="OrthoDB" id="18598at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034315">
        <property type="term" value="P:regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051279">
        <property type="entry name" value="PP1-Reg/Actin-Interact_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24112">
        <property type="entry name" value="LEUCINE-RICH REPEAT, ISOFORM F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24112:SF40">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="700"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="712"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="734"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="751"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="783"/>
            <end position="792"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="858" mass="97892" checksum="5D9D77B109549165" modified="2022-05-25" version="1">MTISENYSISEDLNKYIDDHLGQHYNEKKQIVEWVESRSLKSKRFKKRIIVVGTYRILSIKKGKVSVSVNKNIHFYDITDLLIIEDTIEIRLRQTYKSGDQQGILIRTEKEILQKIILMVRETHRSITHGFADPSFTLKLNVSPDFLLPMSEKVEGVADHFIEVYKAQSNYRNTIPSTQVFRLVEKYVHDNVYDLDLSKCPGMEGRSDLNFNLETLILSLKYNKYFQSIIISGVSQRCIISALGEILATNTNIKKITASNLSEEQTFLTLGTSLSNNVFNTVQVFDFSSTSINQATFSVFCEGLETLNHPILYLNLANCNLHSKSVELLFGAFVKNLKMSCGIEYLNLTSSKFEDIGSSIMSSWIYKMKDDYSLRKLYLSSCSINLLLLGSQLKTLKLLKVLNLSNNRLDRAEVDQIMLIVQESQGLESLDLSNCNINNDHFLYLSETIGKNRKVTSLSLNILNNPIEKKPNILVQSFEYFNSIVSLDISNCNLGLKTLTRVLDSLKNNSNIHSLTLNSNFTGTSNEALEFITELKHFIANNVSLKSVSIAGNHGSMLPQKAIIILLEYLRESNAKLEYLDISYHHLSDQVAPFIANLLEYNSNIKGIELDKNHLTINSYQSILLSLQNNHSLIRFPFPCYDFEKSFISLTTAKKSQLMSIFHRIQNIIEENGSYQVFDPHFCPTPYSFKNEFNKNTGGSGAYLSENGHHGSNQSDHSSSSNNAAALSTSPSSPQISFRATLSPTSRQGKNNYNNKNNYSNHTTTTTTTTTENYYDQVDHTSFPPPPPPPPTDYYYDNPLPPPPVPDRPQIDLQHSLEYRISHLNDDYDQPQQQEQYYDQDQYQDQYQDQYQYQYQDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V640</accession>
    <name>A0A8J4V640_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006277</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000282">
        <property type="protein sequence ID" value="KAF2072419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V640"/>
    <dbReference type="OrthoDB" id="20561at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="117"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="36"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="160"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="307" mass="33999" checksum="C3677B23C4DDB418" modified="2022-05-25" version="1">MISSNSTLNVNTNINEPKRDPLQSPVLKLRGLEMTVYGLEKENLELRQRIESQECKMNGLNKLISEYELENEQHEKRHAQLEEEILELRKQLKHYNPNYLNTSNNSIGIEREMGNVKISNSNNNNNNNNNSNNNNNGNSDKDKFYDDAPLYLYTGRSFPNSPSTNLTNSSSFNNSNNNNNSSNNNNNNNNNNNLTSSCTNNNININSSSLNNSSNSISHMSSSNNNNNNNNNNNMDGSLTPTNLSQPINHNSSVSRSPFQKGKGAPSSPTIVSCVTDGVSSGVNISMSPSPVIPQPRKKSIISLFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6Y2</accession>
    <name>A0A8J4V6Y2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002406</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000066">
        <property type="protein sequence ID" value="KAF2076292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6Y2"/>
    <dbReference type="OrthoDB" id="5589010at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="2">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="1">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="382" mass="44558" checksum="E0A5A718941E9C40" modified="2022-05-25" version="1">MDTNVQRALQEIVKRVPENLNSFIIYDYGVKNLESVVEKIKEHSQDFFLVIIADFQSVNEKKEKEIQDLFAKLHKPYCHFVRALQSPQSIKSVPDHIGTTLATLIHEKWPRIRKIFYFISKDERFESYCAALNMSQKEHVTPVSLNIDEFLKLKFVGGDRIENNLHPRLEHNPDWFKRNKSTPVGRWLSFLGKKDTETEDEYKARKNEMWKLYKIAAGENFNVNITTDYVKPVGVRENIEKHHSDTRFSVYVDKEGEDHIIPFLLKLPEQFHFTIWSFKSEADTKDKIHSSSDHKNITKGATLVVYKSKIGSGNFIYVKKSCIYGKDCKFQQTCKYKHPKFDSPVSSPTTSGVNTQTHTATICKYYPECNFKNNCRYLHPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7F4</accession>
    <name>A0A8J4V7F4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Citrate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_004792</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the citrate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000176">
        <property type="protein sequence ID" value="KAF2073904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7F4"/>
    <dbReference type="OrthoDB" id="435022at2759"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00717"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046912">
        <property type="term" value="F:acyltransferase activity, acyl groups converted into alkyl on transfer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.230.10:FF:000002">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.580.10:FF:000005">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="entry name" value="Citrate Synthase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="entry name" value="Cytochrome P450-Terp, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019810">
        <property type="entry name" value="Citrate_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010953">
        <property type="entry name" value="Citrate_synthase_typ-I"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01798">
        <property type="entry name" value="cit_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004126">
        <property type="entry name" value="PRK05614.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42871">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42871:SF1">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00480">
        <property type="entry name" value="CITRATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="478"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="483"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2073904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="501" mass="56671" checksum="AD0A133073B63BD7" modified="2022-05-25" version="1">MSHLDRINVLASHLSINDTASEINNDDDELQVNNTSAAPAAKKETITVLDNRTGKNYDMKIKNGCVNATLFKQVSETKDDNGLMIYDPGFQNTAVVTSYITYIDGDKGILRYRGYPIEELAERSSFLEVAYLLINGNLPNKTQLDSWSNKIMTHTFLHENLVGMMKTFRYDAHPMGMLISTVAALGTFYPEANPALAGQEIFKSESVRNKQIYRIVGKLPTIAACAWRHRIGRPYNTPVNHLGYTENFLYMLDKLSENDYKPNPVLCRALEILFILHADHELNCSTAAMRHISSSGTDPYTSVAGAAGALYGPLHGGANEAVLDMLLQIGTKENIPQFIADVKSKKKKLMGFGHRIYKNYDPRAKIIRRVAYEVFESLGKEPLIEVATELEKQALSDEYFVSRKLYPNVDFYSGLIYKAMGFPTDMFPVLFTIPRAVGWLAHWVEHLEDPETKIYRPRQVYKGEWYRNYVPIEGRPPAKVRSQDSYSSATTKRYSKLSNNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7U8</accession>
    <name>A0A8J4V7U8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphoenolpyruvate carboxykinase (ATP)</fullName>
            <ecNumber evidence="3">4.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_004360</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>oxaloacetate + ATP = phosphoenolpyruvate + ADP + CO2</text>
            <dbReference type="Rhea" id="RHEA:18617"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.1.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphoenolpyruvate carboxykinase (ATP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.49" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000152">
        <property type="protein sequence ID" value="KAF2074339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7U8"/>
    <dbReference type="OrthoDB" id="68755at2759"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004612">
        <property type="term" value="F:phosphoenolpyruvate carboxykinase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.449.10">
        <property type="entry name" value="Phosphoenolpyruvate Carboxykinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001272">
        <property type="entry name" value="PEP_carboxykinase_ATP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013035">
        <property type="entry name" value="PEP_carboxykinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008210">
        <property type="entry name" value="PEP_carboxykinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30031:SF11">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE (ATP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30031">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01293">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68923">
        <property type="entry name" value="PEP carboxykinase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53795">
        <property type="entry name" value="PEP carboxykinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2074339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="510" mass="56425" checksum="B746496314A1808F" modified="2022-05-25" version="1">MFSTSVIRSSNLLKSSSRLYTTASTATASSSPSLSSKSIYDLLRRKRDRSGLINIQAEQDSEVTTFPRERSGLDYELNWTLNGYSITPYNQAYRNPREKLLLQNSVGTKDKNNVLTVVNSESANTTNYYLDESVSQLNSNSSTISIPNYKAILELVKDNISEGDNIYVRDAAVGSDKGKESTIRAVTNDSVSALFLNHLLPNTNVGSTKNFKHSLTVYLTPKFVLSNPEKYGLKSSSFNIIDVKRGIAVISGNQSTENIRNVISAISSNTLMKTYQDTLPLPTSTIYSANEKTVLAFDSENYLLNNKFSGKVVSQGSIWSVDSGLTRLFDSITYSQSDLAVNQCDLVEKVGKKVNATIPIKFTTNVYQQPNAVVFFVNDKVLPSFAQLNVDQAEKYFVTGFTGENNFYPFYSFNTQSTTPAQDKAALFKKLISVGTNVYIINTNSLKPADVDKLLTAMVNGKVNKASQSDVFTTLSPISSSKSTYTEQVKSSIKEFETKLEKNFEKLTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7Y1</accession>
    <name>A0A8J4V7Y1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reactive oxygen species modulator 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004261</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000147">
        <property type="protein sequence ID" value="KAF2074444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7Y1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018450">
        <property type="entry name" value="Romo1/Mgr2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10247">
        <property type="entry name" value="Romo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01378">
        <property type="entry name" value="Romo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="121" mass="13415" checksum="46E5B95AD23BEE65" modified="2022-05-25" version="1">MPMPVKYNPNDLPTGNKCVEGIKMGFMMGFGVGSSIGILASLFQPKKPFNLFAIRTFRTCLKMGLTFAAFMSIGSLYRCDDADIKNIETSPFLQQIKNKRLNLNSSNSNTSEILYTAPTMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V809</accession>
    <name>A0A8J4V809_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004161</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000142">
        <property type="protein sequence ID" value="KAF2074534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V809"/>
    <dbReference type="OrthoDB" id="10404622at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="9"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="119" mass="13693" checksum="2BA590933C3D9691" modified="2022-05-25" version="1">MSNYLFPIRDEGGGEENEKPIIHGHAPTEKDMPGMTCYQNGYHPSRWELAQVFLNGIWKPSKFKSILQYKNRTRLDLKLNDTCSDATLWHQNKNENVTLYDLMKQNGGKPLVLMCGSFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V842</accession>
    <name>A0A8J4V842_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001220</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000028">
        <property type="protein sequence ID" value="KAF2077447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V842"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd24028">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="521" mass="58909" checksum="9546668CDD15CC15" modified="2022-05-25" version="1">MSFIGIDLGSKNVRIAAKRNGKDVEIIQNQYDEKSILSCISFLEDRYLIGEKESLNYENTLFNFKRIMGLDYSQIPLELMDFWPFTVVNVNDKVNLQVKYDNETKTYSPEELFLMIILKIIETSENLLGHSIENASISIPCFFTHPKRKAILKACETAKLKVRLINEPVAAAMACSFGSGEKNVLVYDIGAYGANASIVTYQDLIYEVKASFGVYSGGEDFDNCLVGHFVQEFKIKYNKDISRDKKAMFRLKCACENAKITLSTSNEASISIEALFEGIDFSASITKAEFEECLIHLFSKVTDPIDQVLKDALMRKTSIDEIILIGGSSRIPKVQQVIQDFFAGKQLNNSLDPDCAVACGAAMYSFSYLSKECLEILLLDATLFSYGIETEGGMMEPIVNRNTTTPCKKHKTFLPIKEDQKSVLIKIYEGNRVLARDNILLGVFEFHLPIPKENIPQKIRVTFNFTKYTETIKVICRHKATGKEEIFNLTHDKVCSKEETIASQNQELSLRRKPLSLKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8K8</accession>
    <name>A0A8J4V8K8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pre-mRNA-splicing factor CWC15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_003554</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CWC15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000112">
        <property type="protein sequence ID" value="KAF2075164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8K8"/>
    <dbReference type="OrthoDB" id="30179at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006973">
        <property type="entry name" value="Cwf_Cwc_15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12718">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12718:SF2">
        <property type="entry name" value="SPLICEOSOME-ASSOCIATED PROTEIN CWC15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04889">
        <property type="entry name" value="Cwf_Cwc_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="40"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="83"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="106"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="168"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2075164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="259" mass="30034" checksum="95C6A6C457360288" modified="2022-05-25" version="1">MSHRVPSRQYSSKAVAAHTVLKSRADLDDSGLKQDKLLDQLREKENKSQEVKRKQQGLEYSIETDKDLLRLKNELIEEEKLKSSNTTTTTTTTTTSTAAKKKNIDADDTDSSDDDDDSSDSSDEEKKKHKKHIDSEQYQDNSDNNSNDSDDSDDSDDSSDEDDEEELMRELENIKREKAEQQKRKEMEKEQEQERIRLERAANGNPLFNEDDGYSTKKKWFDDGVFKNQARDEPVTKKRFINDTTRNDFAKKFLGKYIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V987</accession>
    <name>A0A8J4V987_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PQ-loop repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002873</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000084">
        <property type="protein sequence ID" value="KAF2075839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V987"/>
    <dbReference type="OrthoDB" id="19344at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF40">
        <property type="entry name" value="PQ-LOOP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="334"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="167"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="167"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="192"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="448" mass="51046" checksum="AFF8F554B88FE103" modified="2022-05-25" version="1">MSATSNNFECKTSFETIDIVLGFALTIGIVISFLPQVIVMILRKNVDGLSVISCWFNYANCMATFLNVLFLNWFVFECCNWMTSWVCFENLLPVIQMLSPFFSTLVIFILYVIYRPRLPPYKIDENTKINSEENTVNYNNSNINPNNDEQKSINNNEVVIDDAQHLSSSPSINNNNSNSNDNSPRIDNDSPLSQSSTDAMLPQNQKSKKNFVDQESFHLKKGSNKIETFFIKCDWYSQPFFWGSIIASFIFFFIGVGVCVQYGSKSKSLEGYAFSMGILASVLVFFQWIPQIYTTWISDEIGSLSLSSLCIQAPGALLVVYFQLDAKQNWSTWFPYLATAVQEIILIVICSYYIVRDKRKKKRLAALEQVQDAQLEQEQAEQVKSNMDKGIEPVGDKENFIYHSDNVDADINPSSNQYNNEIIINNADNLNNQINSSNNNNFNINSFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VA19</accession>
    <name>A0A8J4VA19_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002007</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000052">
        <property type="protein sequence ID" value="KAF2076694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VA19"/>
    <dbReference type="OrthoDB" id="194358at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24121:SF21">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24121">
        <property type="entry name" value="NO MECHANORECEPTOR POTENTIAL C, ISOFORM D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="692"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="796"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="835"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="73"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="226"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="562"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="909"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="997"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="73"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="138"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="226"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="237"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="253"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="562"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="629"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="909"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="919"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1001"/>
            <end position="1016"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1219" mass="137151" checksum="592C90BECAEB438F" modified="2022-05-25" version="1">MNNHHLNQNLTFNESLLADIGLGDSMIKDMKSQQQDTYKLIDFALSASAQIPWNSESSESDLLGIDSNNSLTTSINNNSNNGNNNNGSYNSQQQQQQSNTISHQQMLNHHGQQNQQQQLPLPQINPNNSYNINGASFTNLTTTNNNNNNNNNNQYPPLTASLQQFNLQQPQQSYNNNHSNINNLTQSGSSIFLNRSNDTHNLNNILNMNNNNNNINNNINNNINNNINNNNNSNSNSPYFNSNDGYQNNSTVPHSPMLNNPSNNNVYSSVPHSPQQMQPQPQQQQVQQIQIETLRSPPQYSSPMQQQTPQQQYVLPPIQQQQQVLQQYSSPPTPGMNYDMSSPLQYQQPSTPQQPPMIIVKQEGPRLEIVDNYSQPFPIYTVKTHVLVPAPVIKVFNYKGDSNDLMVVANPDPDDSIIISQPAVTCSDGSFEFKFTDMHVDRKKHDPCSSFRLKFSLINKRTFERFNEVLSPSINLFYHTDLLPAPDIARLVPNSCYCGQEPEVTCYGYYFKKGRTEILYVDLKPLNPSPDHQPHTVTQEQLRMPNQTSFSFAFVPPHRATSGTYSVSTRYTKNTTKKDKDKSGKNKSKIGKSHYFSYNDLPEDGNPKRQKTRIDDRGDGGQFDGSDDYYGNNNNGQPQQQQQQNSPYSPPSGSSPAHYSTYMNTIIHGDVDDLVEMLKDNKSALNEIRDNSLNTLLMIAAKEGREDMFDSLIENGADLSIKNRYGHNVFHMACFSGSIELFEKLVELDTTGELLMERDSVGATCLHIATERGHFRLVKHICDGDFDKLVDTKDEKGLTSLHYAAQDGNIRILSIFIERILFLSTADALNCGDFTGMTPLHWAASLGRLAICKKLVESGADVNAVDGDNETPVFKSIVTQNKDITIYLLGKGSDGSIVNSRGKNHIELLAESKNPRIEEMEEDEDEDEMEQDAKESAEQQETPGDFTVGKQLQQQNKFVFQKKQERLHTLGEQTKLQLASQLASQFSKLNLEKNNKYDELSNTPTTTTTTTTSSNTKFETFKPTPGDSILAGELDFTNLTPRDITVNRFQILINQIEKNITTKDHDKNKQLTSLLKQSFTDKVSDWMNSEEELLRSRRAQIEIEEDLSIKAHEIIRLTEFISACPVNLTELKAGSVGLFVLKRERYYGFHQSDVLVIASNDSMSAIKKIYGNQLPSCLLANIVFAVKTQSTKPSALMPHPGTEFFVVDIEVQKQANSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJN9</accession>
    <name>A0A8J4PJN9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_010566</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="AJWJ01001200">
        <property type="protein sequence ID" value="KAF2068108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJN9"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032060">
        <property type="term" value="P:bleb assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904630">
        <property type="term" value="P:cellular response to diterpene"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043327">
        <property type="term" value="P:chemotaxis to cAMP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046580">
        <property type="term" value="P:negative regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110094">
        <property type="term" value="P:polyphosphate-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905303">
        <property type="term" value="P:positive regulation of macropinocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031269">
        <property type="term" value="P:pseudopodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050920">
        <property type="term" value="P:regulation of chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030587">
        <property type="term" value="P:sorocarp development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000048">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="134"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="15">
        <location>
            <begin position="389"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="48"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2068108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="460" mass="51091" checksum="C5FFBD5C046A2D50" modified="2022-05-25" version="1">MGKAFSKEKKNKTKSNKGGKQSKNESNDSLSVATTTNGNNTQTTAVTSEKNQSPNTSPVPSPDLTSATPNNNNQINTSGVNNNTTGTNNSTSSSLKDNGATEVSLDSSNMGEEDEGPEEIIFSKNKQTVTKDDFELLTVIGKGSFGKVMQVKKKDDQKIYAMKVLRKDAIIARKQVNHTKSEKTILQCVSHPFIVNLHFAFQTRDKLYMILDFVNGGELFFHLKREGRFSEPRVKLYAAEIVSALDHLHKQDIVYRDLKPENILLDSEGHICITDFGLSKKIETTDGTFTFCGTPEYLAPEVLNGHGHGCAVDWWSLGTLLYEMLTGLPPFYSQNVSMMYQKILNGELKIPSYISPEAKSLLEGLLTREVDKRLGTKGGNEVKSHPWFRNIDWDKLDRKEVEVHFKPKVKSGTDVSQIDPAFTQERPMDSVVEGSTLGDAVNKDNSFEGFTYVADSILKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJT0</accession>
    <name>A0A8J4PJT0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RNA-binding region RNP-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_010478</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001138">
        <property type="protein sequence ID" value="KAF2068197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJT0"/>
    <dbReference type="OrthoDB" id="434258at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02325">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024642">
        <property type="entry name" value="SUZ-C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36911:SF3">
        <property type="entry name" value="GATA ZINC FINGER DOMAIN-CONTAINING PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36911">
        <property type="entry name" value="LIM ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01424">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12901">
        <property type="entry name" value="SUZ-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00393">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="R3H domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51938">
        <property type="entry name" value="SUZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="202"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="308"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="R3H" evidence="4">
        <location>
            <begin position="401"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="SUZ-C" evidence="5">
        <location>
            <begin position="719"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="488"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="568"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="724"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="9"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="118"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="491"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="568"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="592"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="724"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="772"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2068197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="782" mass="85961" checksum="2CC210AB49681B19" modified="2022-05-25" version="1" fragment="single">THTHTHTHNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGQQPHQQQQQHHKSIPTPSINIANSFQPQPSLFSSSLPPLLGHQFHHHQIEDLSFEDLTFGENDSKSIGNGYGLGNGFNIHQQHQHQQQYQQQQHHLLQSYSNSSFEQSSIEFDSPLKSMLIGGSNIGGSGLGYDNSPNGLMSLKAAKQQLKKEKRPKTDKSDRHAVLISSKGDKINLAVLLKLLEECGPVRAHHFRERQNFGFVQFLDSDSADNAIEKLNNRDIDGQMITVERIKRITPGKDPRVASVGSPQCSSLPTTLPSMIENKPDSTLVLKNLPFNLKQVQLQEILENINPSAPQSVNFHYDNIGVFRGMAFVKYRLVEDAVKVFDALNGTDVHGRRVRLEYKRKVSKSTEIPQEVLEDEQLRKVWEQLKEFKDDPNANEVTFSSFTGVQRMYIHNMAEKLKLTHTSVGEEPNRSIVVSNKKLVVGTPTGRDDSILFSTSPQIPILMGNNNTSSSNQNNNNTSSTPSSFSNSFNILGANGGGSTNATTGSGNSLSTTPTSIIGVGSSNSSFNSTIGFNINRVKVSNNNNNNSNSSSNTPNNILTGFNVLSNNNNNSSNSNSNNNNNNNNNSNNSTSNSVNATTPTSGNNQNNQNNNMFYNSFLNDNSKAMGTSWEKNPNFLSIKGFRDRSYSASAAISTDSPNESSLSSSPTWNTTNSFSNSFTNTNSFLMNNPTFSTNGIFIPNNNNNNNNNSTPSNSFNLVSSPILRQPKGPDGTKGFSDGYRAARKFSQSPLPSSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJU4</accession>
    <name>A0A8J4PJU4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010447</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001119">
        <property type="protein sequence ID" value="KAF2068228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJU4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051441">
        <property type="entry name" value="SelW_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15124:SF27">
        <property type="entry name" value="MIGRATION AND INVASION ENHANCER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15124">
        <property type="entry name" value="SELENOPROTEIN W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="297"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="301"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="326"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="345"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="364" mass="41242" checksum="8D62BC6328A05BCE" modified="2022-05-25" version="1" fragment="single">MINNGTALQLNKDLISDDPSSEKYVSDGIELLERFISVVDEQSTVPVFESMMITDINTDRLTTLKKSLDAQFFDTQGHIKVFPLLEYFFRMDKSQFLSDYFKVDFDSIKTKVSEEPQHFFKKILQQQQQQQQQPPTKEELSPKAKRELSKEPIVNLNQLVEPQLIEQLTKELNSMREQHTKLKEKHKEVLAQYLELAYLAKSEIYDTFNRVDLKIVKEKENEYCIEVLDYPNYKDSIILPPLQFRGLDWKVTIYPEGNTKGGLMSIFIESLSIMKTPFNEYKTNLVLTLINHKEEMSKANSSSSSSPSTTTVPKNSSSSSSSSTPTPTPTPTPTPTPTPTPTPTPTSTSTSTSTSTSTPTPTPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PK75</accession>
    <name>A0A8J4PK75_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein prenyltransferase alpha subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_009326</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein prenyltransferase subunit alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000680">
        <property type="protein sequence ID" value="KAF2069355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PK75"/>
    <dbReference type="OrthoDB" id="1924260at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008318">
        <property type="term" value="F:protein prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.120">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002088">
        <property type="entry name" value="Prenyl_trans_a"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11129">
        <property type="entry name" value="PROTEIN FARNESYLTRANSFERASE ALPHA SUBUNIT/RAB GERANYLGERANYL TRANSFERASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11129:SF3">
        <property type="entry name" value="PROTEIN PRENYLTRANSFERASE ALPHA SUBUNIT REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01239">
        <property type="entry name" value="PPTA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48439">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51147">
        <property type="entry name" value="PFTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2069355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="412" mass="48078" checksum="094A37DB42D741F0" modified="2022-05-25" version="1">MTESSPISIIYKDLNKLFDDNPNIDELGLILSTKDYDSGLESKPFILIESNKLGISFQFVSQLYGYCFTIFNQLRSDFDKLVGANPLSLSLFNNHNDLLKQINDASRNLLLVNAENLSALNMRKKLIIIGYNNHMNEIKTLNLIFTKHPKSGEAWCHRRWVLLDYKKKNNNAMLSSEVELGVCKRVAEIYPKNYYAWTHRWWVLCNLSVKEKLEDLQIIEDWVKRNISDYCGYHHRFLILSSLFNHTNNNNNNNSNNSSAPDNNNNIELQDIISIYTQEVHFINRLITLYPGHESPWTYKRLLSNYLLTGIPLNNKSNISNQIGVNLQSEYEFCETVIADTDSSYYQQQKGFAQRYLCKILDLYTSPNINDTSLKIDDINQCYKENITNLIESFPHQTQFWNYKLSKQTDRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKC0</accession>
    <name>A0A8J4PKC0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010100</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000923">
        <property type="protein sequence ID" value="KAF2068573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKC0"/>
    <dbReference type="OrthoDB" id="17470at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045068">
        <property type="entry name" value="BACURD1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING ADAPTER FOR CUL3-MEDIATED RHOA DEGRADATION PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145:SF8">
        <property type="entry name" value="RE57120P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="207"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="162"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="373" mass="42981" checksum="17A467D7026A6BE2" modified="2022-05-25" version="1">MYSSNNNTPDIVESCQRKNIEESIEGVQSLFLGYFESMQQQIKALEKRKEILIDVTKVIDVSVIPDPITLNIGGFKYQTTKATLTKIPNSFFDLMLSGEINIKPMTNEPNTYFIDRDGTHFNYILNYLRDEGDIQIPEDIRHCVRKEMEFYRINNHFKLDDTATIIDKLNSLKLKLDETEQELVLAKEESIKTRKEVDFLILSESSLILRSNQFKIISDWIDKKLTNFQLLYRASENNSFNTRTFHSKCDGKGATITVIVTTDGCIFGGYNSHSWNSNGYFYGDNRCFIFTLVNKHGIPPTKYLPKENNTNYVSSGSNPMFGYQNGNTDIRIGEKDSYQMFPSTYNDTTGKCYTTLTPFSVFTIKYLEIYKCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKM8</accession>
    <name>A0A8J4PKM8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010437</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001111">
        <property type="protein sequence ID" value="KAF2068239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKM8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="174" mass="20708" checksum="031C24A6C9DAA88D" modified="2022-05-25" version="1" fragment="single">MDQLFFSLWKNKNLLQKIKYSDIDYYIINNIAQLDKQYDYVLSLNLYGIPVVYNIKDLDQYNVYNLQTHKYLITHIIVSTHFYVNHYHIFLQEQQSQPILFGLSITTIDDLMIPAIELPHSIQYMKYTISEPLTQEYIPKSLTSMFIKTFTNIDRIPNGVRTLVMETFDYDPSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLF8</accession>
    <name>A0A8J4PLF8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008548</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000536">
        <property type="protein sequence ID" value="KAF2070135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLF8"/>
    <dbReference type="OrthoDB" id="198417at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053354">
        <property type="entry name" value="MGDG_epimerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43558">
        <property type="entry name" value="REDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G10540)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43558:SF6">
        <property type="entry name" value="REDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G10540)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="437" mass="50585" checksum="F3879BDD266CB1A0" modified="2022-05-25" version="1">MNNNIIHYLNEYEEFHTVYQQPDGYIEKNLNFINQLLGYDTKFRHYPKVSDPYLTYVRSYEVGKTSIVPCYDLFQRSFHVFSNGLLDGIWGLDQEGCQVFCYGGSVLACLLDLPLGLMRSYANLRRYERLIDRFPFPSYYNNGVKQEYSNKHSFTVELRKHFLEAAGQDRNSSFSVSDIDLFITAPSISAAIAKLEHVANTIAANYTRRQIPFRFVRTPNSVTIVSAYPYRPIQIISIFSKSMQDQLNRTDLDCLAHVFTGDNIIASSRCIRSLNLRANYIPSHLFIAEQRQRIFKYATRGFASICYDSNHCQHAERCDQKMENFDLSIGIQRQIDKLNTRLRPHPLQPITYSIEYLTANYPFCKTKTFDDTLEFLHQHFPQQGLICTDIHDALLVPQDLQTSLGKIKPLDWNFNIWKKSIDGDNGDNGDNGDDNEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLG5</accession>
    <name>A0A8J4PLG5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">CYY_009572</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the RBR family. RNF14 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000746">
        <property type="protein sequence ID" value="KAF2069108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLG5"/>
    <dbReference type="OrthoDB" id="10009520at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031090">
        <property type="term" value="C:organelle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20335">
        <property type="entry name" value="BRcat_RBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20354">
        <property type="entry name" value="Rcat_RBR_RNF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000051">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047548">
        <property type="entry name" value="Rcat_RBR_RNF14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="759"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="19">
        <location>
            <begin position="505"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="509"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="158"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="229"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="431"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="173"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="233"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="244"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="285"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="337"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="354"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="446"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAF2069108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="793" mass="90221" checksum="337FAD0C292FAEE3" modified="2022-05-25" version="1">MTSIEQQVDDLTVSKVTPFVEQNINELNSLVQIMFTPSTPDVHCTQKLTKSQNTKLLQYSLCLEKTRQKIVILISNYMAHEAYLNNLLGLMDRIDDILTTIKIVSGQEEELSFDVPSVNSNPADLFDTDNETVFPSPPRELMKKKSKLNLVLFSKLKKKNAATPPPPPPLPVPVNRYRYNESSESESSSSEDENEVSDRRDRSRAFTLPAVIPANPAPMPNRNAGRFILRPVQPLPPPPPPLIVPSRPTTTTTTTTTSKPIRVSRKQRNISQSLSSSLSDTMNDTSEEEEEEENQEEEESEEESEDEGIVSSNQITSLSESESIELSHHRSRVGINNIPQLRTSSRNVLTRNSTTTTTTTTTTTTTTTTTTAGIFGKRKLLLARARGDSTATTQPYQRRNSAPAPVVSRQALHDELIAMVNIYNQMNSDNIQKRRKAQEKKKVDWGSEEEYDEEEEEDEDYSSGSDESSSEEEQEDPQIQKLLLEKSYLEMKKARDTEIPYTLETPVECKICYMEYENSNEVYKFQCDHSYCVDCITAYLASHIVEARVLDLACPDPSCKKELRECDIFLFTDEKNWLKYQKFSMIASLKAEPIKWCPKPDCDTFVRGGSARNPILECPKCKTEFCFHCGEESHEGAKCGKEAMELNQRKGESGTTAAGLFTEWIETNKFNVKNCPQCQSHIEKNEGCNHMTCVHCQHQFCWLCMNDYAPGHYMDSNFPECYDKQYYQPHQLGELHVEPNYRRNRSRRRRRKAAKIAKVALIVTAVTVGAPLILIGGFGYLCYKIHKRRTRNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLP7</accession>
    <name>A0A8J4PLP7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_008391</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000513">
        <property type="protein sequence ID" value="KAF2070285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="24244at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057709">
        <property type="entry name" value="DUF7949"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25820">
        <property type="entry name" value="DUF7949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1271"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="5">
        <location>
            <begin position="384"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="4">
        <location>
            <begin position="591"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7949" evidence="6">
        <location>
            <begin position="978"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="3">
        <location>
            <begin position="1026"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="954"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="954"/>
            <end position="973"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2070285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1314" mass="149210" checksum="73FF92DE6D4818EC" modified="2022-05-25" version="1">MVLDLIVMKEDLDPDISSIKFNDMDFTTTIPTLVQEPYYYYRDLKTDLLFQGLQMLYVEVNGVKISNHSIACVDPIANLVFNYPAQDVIRPSSNSKKYFKLPNITHTILPTTFYTSTSRGPYDYQVKQDPIFQEILFFDFNLVYNRIKSIDIDFTLSWGTSSTKTFNYKSFLDYYTGNTYSFTPNLIQTDKGDFYRPVLYCQVPTPNYRNLTFTLLETDYVAFPVTSSIESTDFIMIPYQNQETSTFVAYDSAGSLSVEAITKTTKINPIPNLSTIISVNLEDKIYPFLKVYAVTTLYDIVQPPSLDYLHYAKYSWHDVPQFIHPLYSLVSGSPVSKFSYFFYKRFPPYFTIQKNLVNTSVMYITELTYISIIVTHNVTDIINLHNGKGQAILSIDISDDYSGFCNLNYNQLFTINSANIVSGDLKAGKFQKIIYISDLFPPTLTIEDHAANSVVYENIYNFKSNKIDKSFNKLFNAFDISLFYFENKNVNLTSNGYLNTLYLNFTTAGPDTTIFFTCKVFSEPINYRDYPFYYNETLGLYSIPFYLPAKMATRDYLNYSIWISSYEINYEAIEAKVGKNSTLSVFNDLQGDEMPPIITNIQFIGNPTTITYDQTLTTPTKIGWVLTIVDSSGLESANFTVTSQLDPIGYNFTLTPNDKIAGTSLSGDYEIPLIFSPSNCTASQVFKISYASLRDVNGRLSQLNARESTTKVRVILNPFYKFVEFNTDVTLTCTSVPTNTISSSFQNLILKQDYDTAKKKLYIAFDIVMEAPLLKNKPLLMLESFIGKEIQMYECESFVMTNNENIYSYNFTCTPPYGFGYPNHIFANPFGIFNRYYYIIGSRFPTTIFVNFTREISLESGTITPSKSNLLKLYGNNLIDLDISICSFDLIFPNRSQHLDYIPPSYFIYQPLALSFLAPAYIRTDFLIIVKSPMGISQEINIIYSGDPYFPSSSESQSISSSSLDQSLSDSSSKGLPCPGNPICGGKEKGTCQYNRCICQSPYTGLDCTSIIIPIDYNPNTTNPNTNITIDIPTSNGEKKDIKLSSLIQVVSLLELDSNQNILQEFIFKEWVFTNITTTVPQYLYETTILHNNHTTYINVTVSFFEYQQFIVFAQQNITMNSNSLKYQVSMSSYEFQSKLNYLQLVMQTNFTSGEEESSCSYTEFVDFENDKEEYLLMQINGNSFNGRFIKTAIIDDRVTTISNTLLDSKYNIVNSDKTTSSFIGINIPIYSKQALIDPDFSLVISIDSAKDKKGSICSSSAKSKLSGAQIAGIVIGVVVITTSAVVVSVYLIYKNIQKKKNIKSMEMKMKQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM06</accession>
    <name>A0A8J4PM06_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">VPS9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_009166</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000647">
        <property type="protein sequence ID" value="KAF2069519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM06"/>
    <dbReference type="OrthoDB" id="20170at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.80">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007941">
        <property type="entry name" value="DUF726"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037191">
        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17920:SF3">
        <property type="entry name" value="TRANSMEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17920">
        <property type="entry name" value="TRANSMEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 4 TMCO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05277">
        <property type="entry name" value="DUF726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00167">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109993">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="554"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="589"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="domain" description="VPS9" evidence="7">
        <location>
            <begin position="235"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="870"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="877"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="903"/>
            <end position="923"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2069519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="997" mass="112156" checksum="AFA6F0A96A576452" modified="2022-05-25" version="1">MSSLLEKEDHNHNKPIGEEVEVEEEENKRLLSSSPESLSQSDFGFLVVSLLFYSFSERDLKRKEVAALLSQTFNFYHLSNYQTNELYEFHSQCNYLKDKKQNYKCTIKDYNINDDDDNNNINNEEFEVYNKQFLKQLSSSKESSKVLKSIKEYCNSIKKDIKKYLDENDIDDEIGSNQFEKFIEEIGNDYQNEHLIGFLQQLAILYPDDDQVAINEAIESFILVNLNGTLFPINKRQDLVLKNILSFAKTRPIQDYSDQNLAQLIEFIAWEDAIAEFSLINIYPAPRDKVLCILRSCRIISKGLSSLGKPFGADEFVGCLVYLIIQTNLSYLYSNVKFIDLFRSSNFMASEGAYYFISLNSAIEYIRERFKSQYEQSPFKEKENELKSSFVYNSDDILNNISIDSKATVSPTTTTITNNIVSTNEDPLSNSSSSLFSTDDMDQDDFKHILNNNNRHHPKFQCIMNKTDEILSNITSLAIKEGIYSSCVRSGLKRFAYKLSPDTFGSNLLLVEVKLVQALLNQVDENSNSNKNNFTRKIKNVFERTTSNHRALKVTLAALAGGGLLALTGGLVAAPLIGAALHGAIGVSILSAISATGLSGTAVTSVLFGAAGATISAERMNKHTSGLNQFELVKVNGSFGLNVVIGALGWIEEEDGEDDYLLWESNLFSSVLCVGETYSLVWENKLLFNLFKSLQDYRSIVENDSKLSKFTSKMLQYAILPSASILDASSIISTSWGKVKDKAQKAGKLLAHEIIASRFGSRPITLVGVSMGARLIYFCLLELVEKKAIGFIENVLFIGAPIPSNELEKWKSIRKLVSGRIVNCFSPSDLLLHYLYDSNNLTDDGISNIACGCSPIGKSTPIFSFFNSKQKEKDNNNHNNNNDSNNNNNSNNNNSSNNSNNNKKVEEKKVAPKKINSKEKMEKKKNSLRFYIDQIENVDVTAAITSHFDYQKSYVQHIIFSHVNINNSEKCPPILIVKPPGIAVLREQQIKEQKLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNF2</accession>
    <name>A0A8J4PNF2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009331</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000681">
        <property type="protein sequence ID" value="KAF2069349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNF2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2230.10">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035927">
        <property type="entry name" value="DUSP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006615">
        <property type="entry name" value="Pept_C19_DUSP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06337">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143791">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51283">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUSP" evidence="1">
        <location>
            <begin position="21"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="212" mass="25002" checksum="A1200FAA0A5D7F53" modified="2022-05-25" version="1">MSSIITSTWGLPLTNFKPATPTIDLSCHDVQSLCSFIDSVSLKEGDQWALINHHWYEHWREKLYSDAPINNFNLVMDDHLRPGISINRDFSIVPHFIWEILKLKYHGGPDLIFTVKRTEDLFSLELDYDIPFYLNCFKSSRRDTIIPVIAFNNETIDSFKRRACNSLKININKCKVWDYYHHSKFTKSPLKEDEVVSKSKFLQNQEIFFEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNI5</accession>
    <name>A0A8J4PNI5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008342</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000505">
        <property type="protein sequence ID" value="KAF2070340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNI5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="243" mass="27580" checksum="D3A582736356CDE9" modified="2022-05-25" version="1">MIEHCDATIGSHFILQDYFLLPILVLSIFVAIKSYQLLQKKTRISIYNSVFFFFAFRALMGTIAYLFAQTNAHVFIKIVFYIYCYTGNLMMVLAFFGGLADLGLIQTHSKRASDFMVIPCGILAVLWFSESLSNTPALYIILNFVVPAVIGVAFLVVQIIYLVRSRITPLHLLWLLPTVALFAYSIYNIGDNYFCVVFFSINSVQFALVCLAVICFAQYACLTRNESAYSEIGSINYEERLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNI8</accession>
    <name>A0A8J4PNI8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ER membrane protein complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_008175</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000482">
        <property type="protein sequence ID" value="KAF2070509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNI8"/>
    <dbReference type="OrthoDB" id="369569at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009445">
        <property type="entry name" value="TMEM85/Emc4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19315">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06417">
        <property type="entry name" value="EMC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="154"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="21"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="21"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2070509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="202" mass="21827" checksum="0D0352845078C0BA" modified="2022-05-25" version="1">MSSNFKNNNTGAYTRQWSLGSTGSGSNSYINSGSASTGSGSSNTTQQASMINNPSKKSGLVPPASTSPPMAANQLTAAQSLELKKKKGWELAKSPAKNILMTGFFLWMIGNGINIFTMPVIIYAVINPIKAIFQVNAMFSRFSDLKDQVMEMKMTYIGIQLLLLGVTLYKCSSMGILPITQSDWISSLPIKKTIEFSSGSWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNN6</accession>
    <name>A0A8J4PNN6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pesticidal crystal protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008365</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000509">
        <property type="protein sequence ID" value="KAF2070312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNN6"/>
    <dbReference type="OrthoDB" id="23795at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.10">
        <property type="entry name" value="Pesticidal crystal protein, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036716">
        <property type="entry name" value="Pest_crys_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35598">
        <property type="entry name" value="ENDOTOXIN_N DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35598:SF2">
        <property type="entry name" value="PESTICIDAL CRYSTAL PROTEIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56849">
        <property type="entry name" value="delta-Endotoxin (insectocide), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="325" mass="37570" checksum="74D9B0A77FBCCFCB" modified="2022-05-25" version="1">MYMAANHVSFKNLLLECIIFFGREDKMELLCKLYLQTVVMYIAFLRDTHFNGIDWGLPEEYVNGVDHIKSMESKIQETIKITHSYYSKFFQKLNPQKDEIQSMMDEGIISYGGYRPPQFKCDFRPLLAQLTYADLTQYNLNQIWLQKTDLDGKVEDDDNSGYNYHYRLPVSNRIYIADFFKMNIMRGSFGSVEYGDNSVNSVTGTKGMRAFQRDGLFYSCFYFTEPKNLTFRVLANTDSNAKIEIVIREIPEGEEVEGPEESIDVATNSHSEKIAYKKLYESSSGPIAEFVSQQYRFEQSGSIYFEAKVLGGNTLDLFTLEIIDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNT2</accession>
    <name>A0A8J4PNT2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007324</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000383">
        <property type="protein sequence ID" value="KAF2071365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNT2"/>
    <dbReference type="OrthoDB" id="23516at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="178"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="218" mass="25371" checksum="2D39607837092B21" modified="2022-05-25" version="1">MKHFQEALVATKNIHVIKGRDLIWRYLLKALPRERKLPCPQCGHLDESAAHTFEYPWLATNILPIVNPILTLTTNTPITWSSKIFKLLPHSLTTASIIGATFKIIWWLRNVKKYEKFTLSPPAQLFEREMKTLQLAALKNLNTKINYLRRKELNHHQSEGLKREIGKNNIYAINFQLNSNNNNNNNNNNNNNNNNNNNNNNNNNNHDNNNRNDDENIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNT6</accession>
    <name>A0A8J4PNT6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007814</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000437">
        <property type="protein sequence ID" value="KAF2070878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNT6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="187" mass="20763" checksum="B0F6462F44F8CE72" modified="2022-05-25" version="1" fragment="single">IQLKPIDPTQNSLFTSLSKFTNKNVNVFINGYGQYFDHTSNETPFPIGSDYFYSQVDNSIIGQQTNSTNTLGKVLSKKILGKLKTTSRGDNEKVVVKENNVVTKSQEVSKMEPPIPAVAVVKNTPITLEPVKPVVVMENIIPVNQTKSELKKSIFDKVSIFEKLTKESKQTIPTKPVAPKRSLSQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP23</accession>
    <name>A0A8J4PP23_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007929</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000450">
        <property type="protein sequence ID" value="KAF2070748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP23"/>
    <dbReference type="OrthoDB" id="34263at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="313" mass="36540" checksum="BD1F182C3FF2C770" modified="2022-05-25" version="1">MNQININFPTQSCFNNYFFTPINNPFQQQQQQYQQQPIQYQPQPIQYPHQPIVNCVNEEECIQNLCSLKEKPATKKRSYVKRKDTTIAKTKTPKTKQPKPRKPSEEEKKHWSNDLLFYNPDIIKINSLINHIDKDVHQDDDYDCCSDDDCFCDDEQVVVVGTHSNPFPNLNYKLESALVKMNENGQFDCPYPGCGKVINGNRGNVSSHLRYHMKFIVPKKPVGGIEQLYLPTGKVRGAILRGQMEKYGSHSFRKDAEGRYVCFFKNCEHRMLTNFSRHVHKHENNGDLVQENLSLVLNHNPIVNWVYHYPHQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPC7</accession>
    <name>A0A8J4PPC7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000462">
        <property type="protein sequence ID" value="KAF2070657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="659430at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF173">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1398" mass="161422" checksum="B76FBC85FD731B63" modified="2022-05-25" version="1">MKGNISWPQRVKSQNPDSKRTTLYSQWQRDNDEEVVKLNVYQDDVFLFKNENIPDDVRGLIFFIGTVYHPNEEEQPFLLNRELYNYYTLKSDLTSIISNLPSQISHLDFVIRSDHIGFFPLYNEKYVSVPSSVTHLSVSGVYLSRDIHIPSSITHLTGDGITKDHNIPSTVQNLYIKGAFSTDVELPHTITSLQVKEVGFNFKTDSLVCKQLKIMKTVHTDIKLTNIYQQFDKLYYPANYSEVQIEYLEPPYFTFSYEQQYSPIFDGVFRVYEYPSLHHYYQIPPTTKTVMWLCKEPLKPDFIPKGVKRLYFGQHFNEPIAANCIPDSVEYIKFKGYNRSLSDIHFPSNVKTLVLGNQFNQFIYGSDELPKSLTHLEIHIDTYGAFHWLYISGSLPINITHLRIKTTQPFQQRLSLPSSLKFIDIKAQSHYWISIMDKEEYYKESLNLPELSSNYGSKHITITLKDALNQTIPLPSNLTSLSIKSVKKKWEIDDYLIDVPEHNFKLSIVPLSLTMLKCPDQFFIPLVNMFTYIEYSIEPYITKIVFKNSDKPISGSNLPLNFDVDTSVFTPKVVDKVDELHIESRYEVFFRFYSGHDNAEIIPVKPNLNIDVKKLNLNLIAIPVGYVPSSVTELGLFSSPKLYPGSIPNSVKTLTCDSINENLIPPSVETFNCFKSLEFSIPPTIKVINLDLNENNLPYLLQQTKRDHTPPPKIINSYDRNNSDSFFVIWRNLYLKDLIMKLKYPSIFIPNDVDQVIEAQNRFINITLIFEDIKLFTKEMFPNNCKNIQGVQIDFYNHNGEHQTDLLSILPRRITRLSIKNFKNIKIPTWITHLELDRSYGDQTPLESHQIPPSVTRLTLLGFRHIIKNMVPSTVVCLGLDTEKIELGSIPSTTTSIQFKKCSLSFTNVFPITVKRISFPIHEFSTVKQLPQFISSRLHNIEFYDKKKSISPKTKSLIWPENEPIFQGQIPNHISRIIFGEDYNQPILENSMPNSVTEIYFGKNFNQSLSSVFLPPSITSITLDGYYRKSLNFEMLPSKVKYLAFYNSCRLKMNSIPETVSHICFFCSNGDTSFPIPPHVEHIKVIQDYGSIDIRRFDNNISFRNLSPVVKSIQIEGFIKFIDKIEPLERDEEELIKPFGLDLQVNLVSKSSGFNDFLKPGSLISNIKSIEFTNSYEQIILPGALPDSVTKVNLPSNFTRQFASPPFLKILHIGNHFNCPIQLPNTIEQLTISNSFSKPFPMRFFPQNLKIITFNCEQFEKEFEAGIFPDSVEEIHFNACYPFAFCENTLPCNLKALSCDYYCDFFEFLPKSIQELNFITKNITYQEGIYFEISAKDLPPSLTKLTLARNQPIQDLEYLPPTIKYLKLGEYFKGKIPNTVETLKLPFDLKKPLAYLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPH5</accession>
    <name>A0A8J4PPH5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">FZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_007240</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000374">
        <property type="protein sequence ID" value="KAF2071448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPH5"/>
    <dbReference type="OrthoDB" id="19404at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001299">
        <property type="entry name" value="Ependymin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10697:SF12">
        <property type="entry name" value="FZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10697">
        <property type="entry name" value="MAMMALIAN EPENDYMIN-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035207563" description="FZ domain-containing protein" evidence="8">
        <location>
            <begin position="18"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="FZ" evidence="9">
        <location>
            <begin position="199"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2071448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="311" mass="32957" checksum="DCDD6049F098E749" modified="2022-05-25" version="1">MFKLFVATLLLVAVVAAQQPPYPQLPTTFTADVSISSSGISADGKFYYDYSAQMERIDSKSPFFGQVTTTLSTYTNMRRYDYADGNCQCGDLTAPQGLYTVLPGSTYQGQQNINGVDVDMWEFNMGQAVITLGVDPSAVVLVRANMTVQTQFQPVETIMDFSNVQKNAIAASTFAVPSYCQCPTPAPPTPPSPTACPNNPPSGCQCVSGLKFCSAVTYPIASATDANMIDGMIGDMFTNWMQSVGGTTPTCQNNMKSFLCATLFPVCTGQVEAILPCPNLCPTCSCGGDTASCAAALNSTSVCTQYGSGFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPL9</accession>
    <name>A0A8J4PPL9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007466</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000400">
        <property type="protein sequence ID" value="KAF2071214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPL9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035311947" description="Secreted protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="508" mass="59134" checksum="890DBD4A31E33FC5" modified="2022-05-25" version="1">MKIFLFFFFILLIFWAKNGLCTYIPTEETLNTINELLISTRTVGNETVFIPIGFSFTDESEVENAFEFLPGTWLALEKELIYVDDTGPIFYRTLKKFSMVQVQEIIFMDELDKEEKDKFHDVTDGFEEDDPATDPLAIIIESYNSFTAAKKFNEYYMYTLFFPPVASLQMLIDQSEGTPIDGGRYRKYKQPVDGKKVSVVKKDIEKYDEGFPNCETIHFFYTLQGDRDKQHYNALLLIKDSDDDITSSLSNKLQRLEAVRYRMLTYYSARESPISPGEGQEGPDNSPTKDVEIPSYQDKVIQCFLDSDTLFYSSTRYQIKLRAIIILERILFPLFDMVCEEDWREDCWDTATSLLTETSVINMAEKGFLMDCISGIVEDDEEMQEKLYGSGQYKNIYENQKYKFSAPIAPLTRNRDVIVETDSSIKVRDYDKPENQITLGMFYDLFLKRSNHGWCTGKQNTLLCMGRINPEKVKDSKRKEINGLIKKIRNDILRYEKSTYTKRSLRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ11</accession>
    <name>A0A8J4PQ11_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Saccharopine dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_007199</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000370">
        <property type="protein sequence ID" value="KAF2071483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ11"/>
    <dbReference type="OrthoDB" id="10059875at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004753">
        <property type="term" value="F:saccharopine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1870.10">
        <property type="entry name" value="Domain 3, Saccharopine reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051168">
        <property type="entry name" value="AASS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032095">
        <property type="entry name" value="Sacchrp_dh-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133:SF22">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16653">
        <property type="entry name" value="Sacchrp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="2">
        <location>
            <begin position="4"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Saccharopine dehydrogenase-like C-terminal" evidence="3">
        <location>
            <begin position="126"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2071483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="477" mass="52618" checksum="50A8752FF2978478" modified="2022-05-25" version="1">MKQVLLLGSGFVAKPAIDHLLTQGDIHITIVSLFQNELDGIVSSFGSKNLTTVQLDIANKMEEIEKFLPNVQVCISLIPATLHASVAKLCIKHKVNFLSASYISPDIAELDKEAKEAGVLLLNELGLDPGIDHMSAMKIIDSAKQNNGKITSFISWCGALPSLECSDNPFGYKFSWSPRGVLASSLLPAIFLWDQHIDQVPAELKFAALQPIVIEHEGQKYEFEGIPNRDSLSYINNYSLNKDDLTTMFRGTLRWKGFGIMVRALAAIGLFNASQDAKLATADLFWRSYLVSSLGCNDNDDDLLYCLKSTITDAFKAQEKLVAESNYHFPIIPRDTDKDVEYAINGLKWLGIVANDEKVVNRGTPIDSLCALLEKKLSYQKDERDIVVLTHQFVVEYPAENKKEQETSSLVCFGIKGGSSATSLTVGYPVGIAADLVLQGCFKTTGVVGPVTPEFYNPLLELLKQEKIEMVETKKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQM2</accession>
    <name>A0A8J4PQM2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">5'-3' exoribonuclease</fullName>
            <ecNumber evidence="13">3.1.13.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_008285</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Possesses 5'-&gt;3' exoribonuclease activity. May promote termination of transcription by RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the 5'-3' exonuclease family. XRN2/RAT1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.-" evidence="13"/>
    <dbReference type="EMBL" id="AJWJ01000497">
        <property type="protein sequence ID" value="KAF2070399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQM2"/>
    <dbReference type="OrthoDB" id="372487at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18673">
        <property type="entry name" value="PIN_XRN1-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12390:FF:000005">
        <property type="entry name" value="5'-3' exoribonuclease 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12390">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027073">
        <property type="entry name" value="5_3_exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041412">
        <property type="entry name" value="Xrn1_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004859">
        <property type="entry name" value="Xrn1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017151">
        <property type="entry name" value="Xrn2/3/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341:SF41">
        <property type="entry name" value="5'-3' EXORIBONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341">
        <property type="entry name" value="5'-&gt;3' EXORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17846">
        <property type="entry name" value="XRN_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03159">
        <property type="entry name" value="XRN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037239">
        <property type="entry name" value="Exonuclease_Xrn2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 13" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 13" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="16">
        <location>
            <begin position="268"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="454"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="524"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1029"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="454"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1029"/>
            <end position="1141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR037239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2070399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1155" mass="133457" checksum="8DE43323E6C33187" modified="2022-05-25" version="1">MGIPAFFRWLIDKYGGLISETTEPRESDGSRSVVDYTELNPNGEYDNLYLDMNGIIHPCSHPETGPKPRSIEDMIESVHEYLDLLFQIIRPRKLIYMAVDGVAPRAKMNQQRSRRFRAALDSRLSKEKEEAEWRDLINSGQKTQRDYDLHKQSLEEKFKFDSNCITPGTEFMDAIAESLRSYVAEKISKDPAWKNVKVIISDAGVPGEGEHKIMSFIRAQRAQPDYDPNLKHIIYGLDADLIMLGLATHEVNFDILREFIQIVKRGVCHKCKKQGHMAIECPMTEMDMMNEKKDFLVKNYQILHLHLLKEYLELELKVSTPFGFNIDRVVDDFIFLCFFVGNDFLPHLPTMEIKDGAIDRVVKVYKDLLPSFDDYIVMNGEVNLFRLSRIFAALAASEHELIEKKIARERDHLARKKSMVSNVAAPPKSLDLNKQVSINHKEAASKFLSEMFKPVEEDKDNAGEEERPSKKLKSNKEAAILVQQKIKEETTTTTTTTKKSNKDAATKVKVKILEQQEALNIETTSIEKNTKKPAASKKRKNVEEQEEQEQAPADKDEGDKAIDNTITQNRAQTEDEIFFDNSKNVKYGEEGWRERYYDAFFKFQDDDHIKQVCQSYIEGLVWVLKYYFKGCSSWGWYYPYHYAPFIGDISNFIDEIPYPTFTIGEPFKPFNQLMSVLPTASSKFVPKPYQKLMGLEVDGQPQEDSPIINFYPTQFLIDRAPTQPAYKGVCLLPFIDETLLLSTLATREGELTQDEIDRNSLGHDLFFIHKDDPINEKFTSGQVLVLEKEFSNQFGSIEDCSALAKKKMPPLLDAVVYKYNNATLPKGYVYNFNTLSGAVVPAKTITAMSKRPNHFHQNSASNRMVNHYQSNGQYKNRHHNNQNQNQNYNANYNSNYNANFNQNNSNFNNNYNNRYNNNNNNQNNFNSNYNQNFNQNYNNTNQNYNNFNNNNGAVSNFDNNQGYNNNNFNNNFNNNNNFNQNYNQNFNQNYNANFNNDGFNSNNSVSYTQTQTGFNNGTNTNMNYNGNFNNNNNFNNNNNFNNNNNFNNNNNNNNFNNNNNYNNNNFNNNNNYNNNNFNNNASKQGFNNNNAGHGKNYNRNYNGNFNQNTNNNNNGNNNNNNNNRNPKQIQQQQQQQQQQQQTRVPMKYNPFAKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQT7</accession>
    <name>A0A8J4PQT7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008167</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000481">
        <property type="protein sequence ID" value="KAF2070519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQT7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="129" mass="13535" checksum="0F5D6A605950F61F" modified="2022-05-25" version="1">MSHYPNHGYPPGAYPPGAYPPGAYPPGAAPPGAYPPGAYPPGAAYPPGAYPPGAAYPPGAYPPGAYPPGSAYPPGAYPPGAYPVGHAYPSHHHHGKYKHKKYKHKGFKGFKKGFKGFKKGFKKGFKGFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQT8</accession>
    <name>A0A8J4PQT8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Acid ceramidase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007192</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000369">
        <property type="protein sequence ID" value="KAF2071497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQT8"/>
    <dbReference type="OrthoDB" id="189997at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.60.10">
        <property type="entry name" value="Penicillin V Acylase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047794">
        <property type="entry name" value="C45_proenzyme-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047803">
        <property type="entry name" value="DCD1A/B-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040521">
        <property type="entry name" value="C45_proenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35190">
        <property type="entry name" value="PROTEIN DCD1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35190:SF2">
        <property type="entry name" value="PROTEIN DCD1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035223393" description="Acid ceramidase-like protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="432" mass="47873" checksum="D56042919652352A" modified="2022-05-25" version="1">MMMKLFVSIFVTIALVLSSQNNYYGVQAVCNGVPNVNGIWNDTLVFNSSVENGKLYTAGSLGNQVPVVHVYGTPYEMGYAQGLLLKTQIQYVYANIFKYFDKEIAGFSGVPEWAVKIIEDGGLSLVLDMVAFGVKDHTPERFFQEIQGIADATGVDKDEIIRVHMIPELLRASCSMFGGWGSATPNGGLLQLRALDWVVDSPLTVNPAVVVYHPSEGGHPFTILSWAGFIGSLTGYSGYTGVSEKVWANYNGTFNRFGTPFYYVMRDILQFDTTVDQAIDRIFNTKRTCAVYMGVGSNSTNTFTAVEYSYDTVRIFDDTTPFPIYSPQPAPHELIKNVVYIDPDYKSAQDQCMTDLLNKYYGDLTTENTQQLIALLQTGNTHAAIYDYALNTFTVSVATQTGSWPVTSPIANAYTRQFIKLDATQLFNEPKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR43</accession>
    <name>A0A8J4PR43_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000261">
        <property type="protein sequence ID" value="KAF2072648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR43"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="3"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="110" mass="12923" checksum="0A3D58DE7B559EAB" modified="2022-05-25" version="1">MIQINTSNTREDLEKDNERLQKENWEFRNHLKVGCSNVDHNSLYVQLETSNNENDKLKEENKVIKQDLEKSLEENDNHNNKMNLLLNKIESLADKILSMAEVIESVNKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRV4</accession>
    <name>A0A8J4PRV4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010295</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001022">
        <property type="protein sequence ID" value="KAF2068381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRV4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="95" mass="10768" checksum="78B3CE9A30346DE4" modified="2022-05-25" version="1" fragment="single">KQFVELDPDFSVLVSYDQKSSQHENTDECNIDTESNTSKPKKKYVLPLIIVGSVIGATIIGVGVFVILKKNMALRLKFLKFRQSRSNSTKMKNFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS89</accession>
    <name>A0A8J4PS89_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carbohydrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_006204</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000276">
        <property type="protein sequence ID" value="KAF2072472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS89"/>
    <dbReference type="OrthoDB" id="21537at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF5">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035155078" description="Carbohydrate binding domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="3">
        <location>
            <begin position="423"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="2">
        <location>
            <begin position="664"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2072472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="793" mass="87386" checksum="A28E1599338010D8" modified="2022-05-25" version="1" fragment="single">MLNKLLSVLICLLFISTIQSASIVDIIAPPEDNYGSVNGCGQKMYQIIVNSAVPVNIDSVNGVAYSSKSLVNGTITIFDVAINFPNLGLNDPSITLIDDAGNTQIFPSAITYYCMTVPTYTLATNGGWSAVVGNGFHVWYKFISLNGLSKPTNYDALTCSVQAPFMCQIMQYPTILTSYYFLISMYMTNENVAYSLPINIEIANSYGTNKLIFPFNEIPAGPSTISNIQIYPANNTVVTTDIMSDAHFFFDAPNLNVLPSIGGGFYYKVNINIPMLGNPTNASYFAFQPAPYSPNPIPFSLEALTKTGVTKLTPDHFIKFENPYAPYIENVDPTSTSSDITELPGSKVFSLKSKSTRYNAFITGAGNYSNFAIKVGYPYGITNGNLKDFYHACSILADSYSSGTFSINCGGYYFDGLQNPLATVDDANPSLSNVEIFGLDAEKVLIRVYANDDISGVQTITYMGGTAIETMTNRDIVKGTKMNGVYEYISTYYSYSQKSPIFTAYDVKSNKLKFESNRAIINTVTKQRVPRFPIYDLIESSNLQSFNFTFFQFKYNDVDLSTNGVNNTLYFNITNAHPSMIPRLYVIPSLMDAFEPSVSAANSFIEGMWNATSKLYQVDFYLPPRVFTGKVIYSIAMSPNVWDSNMLESVFGAQSQLRVSSSNGDLMPPVVSAYAAYPSTAVNVVADTQIGWTITIQDLVNGLKSAAFNITSDYDLEPFTIKVTPANAVSGDKYTGVYNIRIPVSVNCRSQSFRISSIVMTDTSDHSSFYPSARQMNSLYKFIHSDQHFVNVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSD3</accession>
    <name>A0A8J4PSD3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000360">
        <property type="protein sequence ID" value="KAF2071584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSD3"/>
    <dbReference type="OrthoDB" id="10456300at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="74"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="219" mass="23696" checksum="BA5B116303C168F1" modified="2022-05-25" version="1">MISGVTSHQSRLNNLMEKENSGGLSTLSLKKSSTTLSSSGGLGSGPIKQKAFGSSGAQVERRALGEITNKNKQSLPTSKKSSGSSDNKLLFVPSSTTPQLKKKINVTNTPSLILANNTKVESKKNNYLPYDPIYPAPPAPLRVNSTLSNKAIESFVMTPSLDIPISSFTDTFERDVYLAESLSFTTNSSELCFDDPSSTDSLMIEDNFSFSDYYNDIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSF2</accession>
    <name>A0A8J4PSF2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RanBP2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_005018</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000190">
        <property type="protein sequence ID" value="KAF2073668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="21674at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025574">
        <property type="entry name" value="Nucleoporin_FG_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34491">
        <property type="entry name" value="A-TYPE INCLUSION PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34491:SF82">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_21, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13634">
        <property type="entry name" value="Nucleoporin_FG"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="6">
        <location>
            <begin position="1021"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="6">
        <location>
            <begin position="1147"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="6">
        <location>
            <begin position="1281"/>
            <end position="1310"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="6">
        <location>
            <begin position="1410"/>
            <end position="1439"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="6">
        <location>
            <begin position="1544"/>
            <end position="1573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="737"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="962"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1056"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1180"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1313"/>
            <end position="1409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1498"/>
            <end position="1538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1609"/>
            <end position="2024"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2040"/>
            <end position="2165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2198"/>
            <end position="2291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2303"/>
            <end position="2776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2788"/>
            <end position="2860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2933"/>
            <end position="3062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="737"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="765"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="962"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1004"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1067"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1090"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1193"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1216"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1326"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1348"/>
            <end position="1382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1384"/>
            <end position="1399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1511"/>
            <end position="1521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1529"/>
            <end position="1538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1612"/>
            <end position="1629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1630"/>
            <end position="1644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1652"/>
            <end position="1715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1724"/>
            <end position="1750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1759"/>
            <end position="1798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1809"/>
            <end position="1845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1846"/>
            <end position="1862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1877"/>
            <end position="1895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1899"/>
            <end position="1908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1924"/>
            <end position="1934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1935"/>
            <end position="1952"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1966"/>
            <end position="1982"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1983"/>
            <end position="2010"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="2011"/>
            <end position="2024"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="2052"/>
            <end position="2124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="2198"/>
            <end position="2208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="2209"/>
            <end position="2262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="2263"/>
            <end position="2278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="2303"/>
            <end position="2316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="2329"/>
            <end position="2642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="2681"/>
            <end position="2698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="2712"/>
            <end position="2729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="2730"/>
            <end position="2744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="2751"/>
            <end position="2766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="2933"/>
            <end position="2946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="2977"/>
            <end position="2986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="2996"/>
            <end position="3034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="3042"/>
            <end position="3052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="3053"/>
            <end position="3062"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2073668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="3062" mass="332037" checksum="3DAECC394292F8C2" modified="2022-05-25" version="1">MDTTTTTTTTLQNRDLIAHLKKQIENPLFQNDSIHQIIKAYLQLTDITQEELQECQQYFEKLKKLGIFQPNHKELKEIQIDLYIKNDQIKQATDLLLNCLKIYPDSLNFFLKYLNILLNSLNYKEYFKMINQVKNRFIKSNQFWKILNQQLNTIIEKENIDQEYESGQKDSLIIQGSITLIQFQLLVNESIDHCIRLLIISFFGYTTTKSLTSNNSNNNSNDNNDNEMIDKFLNQFKGYIKLFKTNIKQTSRITNNISSSESSLIDEYTSRYQYYKGIYKDYISILDRTGNDDGDSKPKLSSIKYRHNPPFENYLQALLQPQHSSQEDDTDISILYLYSEVSRQLYDSIHNQHDIKLNFYSKLLNQQQQHYPYIENLMNQDLDVSEDLELLDNAYLSSIETSDFNLVSKNPLDFQRFIFFSSWHKRQTNNYSTLVRWSFTLFKNFIKRANSCLNIENQSNQSVGIVHIVVFLVNLIYETENLVHSDNIMDVYIDNHLLIPFNRQSFHYKIWNSMIYYSSSDVGKTMVQGQDTFSINTLIELLNGILRNGNYLGPLVAYRIANDLVDIVDVCMAHVNDPLCVGTDIDVPRLQAGYYRITLTSLTQLKPNDTVNNQVLSEDLIQHIENICNFELAMLYKSRSSFKTALSHILKVETPHGYIEALQLCKEIVLSSNSIQNQQQMKQIISLMESIWNKSQDFNINDFDDTFDTDRYLSLIDDFLNTISNFKTQFNIFDTTSSTTTASGSKSTSNSSAGKNMIPNHSIYGNNNSNGNTGNSSISKFNLPSNNNNNRSLFLSSTFSPVKSIDNTIGSSAFSTPIKYNIPGTPQKKSIDSLDISLLNNNNINISAKLEQEREKEEDLSLFKNSFLKRRKERRELLNSLKDKKKSLVCQVDLNAPLLDLDSTISTSTSASTAFAQQQQKPSSSFFGGASTTSSSMFGTTGSTTTTPFTFGSATTTTTPSIFSSSSSSSTSSPAPTLFGGVAKPTAPTTSLFGVPSKPTELQTSTSPTSTTSVSTSSTTKEEMIWECPCCEAKNEEKANTCVTCMINKPKSITYKPKSQKDEPKSMFNNMPTSTTFTANPSSLFGGGSTSPSTTTTTTFTANPSSLFGGNTSSSTSTSSLFGGNNTTAITTPNNKSPTTTPATGSKEEMIWECPACEQKNDEKAITCSLCMINKPKSITYKPKSQKDEPKSLFNNMPTSTTFTANPASLFGGGNSTPTTTPSSLFGGNSASTATVTTSNPSSLFGGSASTSSTTPTSTTSIFGSAAPPKPSSPTTTGSNEEMIWECPACEQKNDEKAITCSLCMINKPKSITYKPKSQKDEPKSLFNNQPTTTFTANPASLFGGGSTTTPIPTTSPVSFGGSNGTAPPTTLTTPSSLFGGSASLPTTTPKQTQESSSATTTTTPPVSNEEMIWECPACEQKNDEKATTCSLCMINKPKSITYKPKSQKDEPKSLFNNMPTSTTFTANPASLFGGGASTTPITSTTTFTANPASLFGGNSTTATPTPTPTLSSLFGGSATSAPKEENKSSSTTTITTPATSTNEEMIWECPACEQKNDEKAITCSLCMINKPKSITYKPKSQKDEPKSLFNNMPSTIPSTTNFSSLFGGAASTSTPSTTSSLFTTPVSTGKSLFSTPITSTISLGSKVENKEDYEDEEDEDQDYDEDDQEDYEDEQQDDDYQEDQDQEEEDDDDEDDGEFSEEPSSYEEEEEEDAQYDKPKTLFSFGSFNNPTSTNTTSPPPASQATTSSVFAPPIFGTPFSASTSTPSSSVFGNKSSDSTPTTASSTSVFGSSSVFGKPSTSVFGNKSSDSTPTTASSTSVFGQSSTSVFGSTSTTPSSTSVFGQSSTPVFGSTTSSNAPSGSVFGKTPASSVFGSAVANTSTTSATPSSSAPVFGSTFKSTNTVTFGSVAPTTKEEEKKADVVVAVTPTPVAEKIETKPDEKELEKKEDVVVTPVANETKQENDVPSTTTPSSQDTKPNDSPSTTTSSLFSTPVSFSTSSLFGGSSSTEPTKTSLFSTPVTGTASLFGTASLTNSLFGSFNKDVKKDKSDTDEDQDEDDEEEEEEEEEEEEEDQDDQDDQEQEDEIQDGQHDQDDEDDIPIEDNDHEDYKEEEEEEEIEQIEDPSKPKPTFSFGNYNNTSAPPPPPPMPANGTLPTIKPAASVFGNGSTNSTSVFGNTTSSVFGGSAPKSIFGSTTSTSVFGSSTTAPTSVFGSSTSPTTSVFGSSTSPTTSVFGSATPTKSTFGSSTPPTSVFGSSITPTTSVFGSNTSTTSTFGANPPAFGSTSSVFGSNSVFSANIQSAFGGSTSTPTTTLKPAIPIPTFDNSVESKQDATETDIPKQDEIKKDESKQGDSESGEPKVEESKESVEPKVEESIESGEPKVEESKESVESKVEESKESGESKVEESKESSEPKVEESKQDDSESGEPKVEESKEPSEPKEDKIESTIESNEPKDKESIESKVDESKQGDSESGEPKVEESKESDEPKVEESKESVESKVEESKEFGESKVEESKESSEPKVEESKQDDSESSEPKVEESKESGESKVEESNESGEPKVEESKESGEPKGEESKESVESKQDESTKEDIVEPNETKETIESKQDEPSESKIDETKQDDSESGEPKVEESKESAEPKQDDSESNQDNSTIKAIESKESSDTQTFEEESKQVETQQDNQTENKKDSSESEDTTKQEEENQSNQVESSKAVSDLKQKEDTTELKVDQDTKILNSLTICSVENAPSLSKPVESLEKEEEKEIEEKPESSLPNTTLKPSTFAFAPSTFSFGSSSTTSSTFGSSPTPSVFGSNSAFGTTTSAFGSTSTPSSTSAFGSTSTPSTSAFGSTTTSSPFGSAFGSTPTSSTFGSVFGTSTISPSFGSVSNQPTFGKLPANQPTSAPPSVFGGVYTPATTNPTSVFGSGYTVPTFGSLATPATTTSTTTAAVNPTVPPPTFIRNTRNDEDEEQSSLIAPPIRQDDSDEDDSIDDESPKSDISITSFMMSPSSLSFDNEPKSTTPQKSSLHSAFSEPSTSSLTPLKPFFQLTKSPSQTENLSEQKQEDDRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSQ9</accession>
    <name>A0A8J4PSQ9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005574</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RCAN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000226">
        <property type="protein sequence ID" value="KAF2073105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSQ9"/>
    <dbReference type="OrthoDB" id="17212at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008597">
        <property type="term" value="F:calcium-dependent protein serine/threonine phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006931">
        <property type="entry name" value="Calcipressin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10300">
        <property type="entry name" value="CALCIPRESSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10300:SF14">
        <property type="entry name" value="PROTEIN SARAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04847">
        <property type="entry name" value="Calcipressin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="172"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="192"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="238" mass="26916" checksum="A9C9D4A27362D99D" modified="2022-05-25" version="1">MEPNMNINSSTDSIEEELSKLILDCENDQDNDSGGDNATSEKQELLNQMKQKKLQLLSNRCPTNVLIIENVDDERAQLLISLLGDENLHSSFNILLLRYLSILNSIILVFSHHDESTLCKMLIERQTFINTTNILIYYGKRLETETVNKYQLLPPFPVRQYLISPPLSPPNEWAAPGDGMETPPNDIDPLASSNNSNNNNNNNNNSLDPEQLSNRLRKVIYEDTTSSGFPSITLEFCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTB4</accession>
    <name>A0A8J4PTB4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phorbol-ester/DAG-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_006029</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000260">
        <property type="protein sequence ID" value="KAF2072650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTB4"/>
    <dbReference type="OrthoDB" id="21001at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00029">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14689">
        <property type="entry name" value="PHORBOL-ESTER_DAG-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14689:SF2">
        <property type="entry name" value="PHORBOL-ESTER_DAG-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="4">
        <location>
            <begin position="7"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="4">
        <location>
            <begin position="64"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="310"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="979"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1164"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="310"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="981"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1164"/>
            <end position="1215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2072650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1235" mass="138203" checksum="7E46A93A6DCA9420" modified="2022-05-25" version="1">MDPLPLQHDWAKKHFNTPPYCFICSKLIWGAGKLAYYCKSCHVTCHYECNTKSEHIPSFCTVVKHDWSKDSFDTNPTCSGCNNIIFSHKGKAHGFSCSKCNFKSHKNCKNLCGRCESTKSSTSLLSLIKSSSESSSKSSGTLESLFTPFNMSIDLNNPVISPSGAGDPNSPNPNRLSRSVWSANQQLSASSNNLPVTSISISSSVASIPSSPNLDSSQYRSLARSVDLNSPRFNSLVASGSLSIDANASIAGGLDDVMFELDNTFIPRSVDQKRQFFEKMKFFLLCFSYRLDPEYNLYINDIKQQIQQQADKLSSSTTSSSSRPSSPTPTPTSTPTPAAVSPLDFFYGDMVKYRSISQVFHSEMLTSGRVRSAKQYDHYKSLFECLLIMYSNYWDEDSDSLYRLAMDIYTFLSTHKVDPENILIGMTPSKEIQRRVKKHFYPTHLDLQQKAPSKFKGKWDLRISRSSLIDNITTWGQIENKDSLMKVVKEFSKHLENEKYKEGDIIIAKLPKNWNDKQQASSVNDPLSSSSSTPLLGVISDSNHHEFDEGSFILGRFIKEADPLGLNCLIYIQADELKPLRIPTSRLVSFRILDIIVPSEKNRIERLITNPFDAFITELRSAVKETTKARLAERGYNVALIGQGLLFNGVQCSNIEGLNKLRKMISSFDSLPEYLTSIDTFSHEATQFFLKYLKSLYMSEMNGNHCIYFNREETLNHPIINLDIIQIETMSYEYDLLHNSSNLNNAYNSNSSNSNSGNSNLDQDPSFKYFSSLKNQYIKFKPYLPNIKSMINEIETGRKYCITAKDSLTGLVSECDLAIDTINNTNTRIFDSTKKRELTNWYLQLIRETIDSYLKETGYDGNAVPENYIDRPPQSLSKVRENLRIVNLVLKPNNKVEFEHRLQRLILPLKKKIYAGIDEFLSAAEILFVEKLKEHKAMLESYSDEFVIVDDIQECDLRSTLALLKSVYLIISNSPVLTPTGGNNNNPGSSSSSPVSQSSSPLYTTASTSPSSPLSFSTLSNALRSSSGGLDQPIIATGVPGNHIPIVLKLYNLVEDIVYIREMRSLEEKFYSNEILSECTWVNDKQQQQQNQALSNIVPITGLIFSRLLRLKHELRTVLEVWESNKLPINNYVPLNSSPSSQSSSSTLSSTSSQSLNVSSLSISSPSSSSSSSLSSSTLTAGNSNSNNSLSPSSSYTSLPSTINNNNNNNNGLNNSRVNHQIIIGKKDDLYISNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTW8</accession>
    <name>A0A8J4PTW8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CobW/HypB/UreG nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000198">
        <property type="protein sequence ID" value="KAF2073540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTW8"/>
    <dbReference type="OrthoDB" id="258627at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051927">
        <property type="entry name" value="Zn_Chap_cDPG_Synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43603:SF2">
        <property type="entry name" value="COBW C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43603">
        <property type="entry name" value="COBW DOMAIN-CONTAINING PROTEIN DDB_G0274527"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobW/HypB/UreG nucleotide-binding" evidence="1">
        <location>
            <begin position="305"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="550" mass="62192" checksum="961328294434B099" modified="2022-05-25" version="1">MAKKGAASKQKKVVKKIPSLLVVGFTQSGKSSLYHNIVKSLKKEKVAFLFDTEKLPESVLKTENCRVLVEDLVISLNNGSMVTVIDEQLLAEFIHDQAVPFDYCVFISGSFDSTILLDLLQCIDEGELAKLADKIDMYNVICTIDCQTFISDLKSKDLLKDRFPIFEDIFKNSLTLKEDNQDSCCDENGVCSKQESDCCNENGVCSKTTTAPEKKDSCCDEKGNCTKEKADPTPVKKDSCCDEKGNCTKEKATPAPVKKDSCCDEKGNCTKATKQPEAKIEEIDSDEEEEEIIEGEIFDDEFDLVKKSEKKISSLIFDQIAASSTIILNKTDLVSKEELEFIQKLLNLISSSRIIITTHSQVGIPALVERAFTIRDYKQENQSLNGLSIVNYYQRKPFHPDRLFELMYTVEGDIDMANAFKGVLLLNGFIWLASDMEQNYIFEIEEKEDTVCPGRPFYSTLKESEWEPMFKEQIKREISLFKYQDRKTELSIIAIDADGFSSEALISKLDKCLLTDAEMELGPNAWKDYENPFAKLGQPLEDEDDYEDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTY8</accession>
    <name>A0A8J4PTY8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Saposin B-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_005326</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000209">
        <property type="protein sequence ID" value="KAF2073356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTY8"/>
    <dbReference type="OrthoDB" id="69496at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.225.10">
        <property type="entry name" value="Saposin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008138">
        <property type="entry name" value="SapB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008373">
        <property type="entry name" value="Saposin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011001">
        <property type="entry name" value="Saposin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008139">
        <property type="entry name" value="SaposinB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051428">
        <property type="entry name" value="Sphingo_Act-Surfact_Prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11480:SF3">
        <property type="entry name" value="BCDNA.GH08312"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11480">
        <property type="entry name" value="SAPOSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03489">
        <property type="entry name" value="SapB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01797">
        <property type="entry name" value="SAPOSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00741">
        <property type="entry name" value="SapB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47862">
        <property type="entry name" value="Saposin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50015">
        <property type="entry name" value="SAP_B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035185835" description="Saposin B-type domain-containing protein" evidence="3">
        <location>
            <begin position="19"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="4">
        <location>
            <begin position="28"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="4">
        <location>
            <begin position="125"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2073356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="205" mass="22500" checksum="9815BEC1C36ED0B4" modified="2022-05-25" version="1">MLKSLLVVILFLAVFGNAARVPPKEGEITTACEICELGVRFIIEVAQKNSSVTYVTEELQHLCYIIPRGLQEACQQVISQYTPDLIQLIITSDNPEEVCSNQLPICSSSSASDSSYYAEENVGDDVTPCKICIYILNDALNIINNGGFISEHLTLALNVDCFKYMPTSADTSTCRSIVNTNMNELLQYNKNGLSSQAACAKCQYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU46</accession>
    <name>A0A8J4PU46_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_003864</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Late endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000127">
        <property type="protein sequence ID" value="KAF2074838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU46"/>
    <dbReference type="OrthoDB" id="9977282at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040057">
        <property type="entry name" value="Spe-39"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13364">
        <property type="entry name" value="DEFECTIVE SPERMATOGENESIS PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13364:SF6">
        <property type="entry name" value="SPERMATOGENESIS-DEFECTIVE PROTEIN 39 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="192"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="369"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="34"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="192"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="209"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2074838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="601" mass="68556" checksum="FB82F456D903F67D" modified="2022-05-25" version="1">MSSTSTPVKGKTGESIIDKLVDKLTPNKKQPVKNNANNSNNSNNSTTTTLPPLDTTTTTTTTTKTKTTITSSSSSSAIASFVNSPQTPQHTSSPSPSFDAGASGGGGSNSGYSTPQKQQLQQDDHEKYLTLSPDSVVVINKDKLDSHYINSIESQISLLNKQQSKKEKPKRSSFKKQVSFNNKLFIDASSINSNEDHQPVNTSSNQFINHTSKSNHVNNNNNNNTPTSTTSSSSPTPTIAQINHQITPGGDEQEQQQQDPNALPFSIQDIPISCRLYIDHLKTENYELKRQLNIVLNDNAEQWEQSIHLQDIINQLEEYTDQQDQWVTELRSELINSTELLLNHHQKIDNLSDILYGIGEQKESFQTLYEQSQAELSKVDEDNQRLTLELQDYKELTNGLDKHIVDMNKELRHLKDHTIETILWRIKNQEEPSGIEAVKSFGGKEQLLLQALKYYQYDYQEEPLLTVIMYLKNTLHWKIFIQILRNQFECLDYYIKYCKLLNRYDDLRLIYRDTHNSLEEGLVMVKQAMLEKDVNLQLCELHTCVAFFDNHDHLHFYKHTLMKCIESLKNGINFTVPFDLLNPQQPSHRSNILAQSLPITR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUH8</accession>
    <name>A0A8J4PUH8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pesticidal crystal protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008875</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000592">
        <property type="protein sequence ID" value="KAF2069806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUH8"/>
    <dbReference type="OrthoDB" id="22869at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.10">
        <property type="entry name" value="Pesticidal crystal protein, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036716">
        <property type="entry name" value="Pest_crys_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38082">
        <property type="entry name" value="ENDOTOXIN_N DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38082:SF2">
        <property type="entry name" value="PESTICIDAL CRYSTAL PROTEIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56849">
        <property type="entry name" value="delta-Endotoxin (insectocide), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="633" mass="72571" checksum="BDFD6C7FCB5FA1DA" modified="2022-05-25" version="1">MTKGLGKKFKAECDAFKKSAKENVKEKYEGVVNDLKEDPFSIIDIEMVGEIINDPLGFITDLVTDKEFIGQMISAAIECVPVVGPILSSVFSLFWPEDPKMKTLTVEDLDKKLAEFKEEMVKIMDDKIKASEKETWKELCKAFFEGYIYQIDQMKKNIVILKREIQDHEGVIADVPEASKERIRVGFLSLFESANDISNFCKKQKFTNHVIEVYMQTILLQCVSTIQLCSFWYQIDLDKYYILGVKKEKEGDVEVKSIKQEYHDLLVECLKNITPAVYSNLVNSKTELNKYYNNNASKMFNTNPWIYPIPLVIAPLNVPKVAPDQYLRGSTPTINVPLEQKTPFIYRFDGANMLPDQMKEGLNGYRQIPEIDPLTQCKGTLIDFSNSINISLPERKNVTIRIFGIYPKSISELYVDTEQLKIVQKCVQDSMFCLPPSFKRGEDYDFRIEPKKNAGFAEGLYHEADGAKSGFTYLKPFNYVKTICLNIRQVTPKGVDILNRGCIRFIELIISDLSAKDLEDFKNIKLQVKEKSDKLDKLSEIEILTKEQDKEFTELTFELITNWSGDWNNFFLFTLHFYQDLNDNLPSSLTELMLKTNLKPLKTSTSQFPPNLKNLALSCSDGVLDIAPTTINH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUP6</accession>
    <name>A0A8J4PUP6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000200">
        <property type="protein sequence ID" value="KAF2073505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUP6"/>
    <dbReference type="OrthoDB" id="21220at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="139"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="268" mass="31173" checksum="C2112EEFCE22188A" modified="2022-05-25" version="1">MDSFRITLDHIKEDLKGLERLDENKHFQDLILNFKKRDQDKSSNQENDNSQLDQQQQQQKTYDIFDLILNNVNIDEIENDYLYIREIDYRLIQNDIKREEENDRQEQNNSNSSTVKDNADKNNNNSSSVDDNDDDQDNDTSSNSSKNSLDNQDNQKDEKIDIDNEMKKEIKENKQKMIKTLIELGEHDDVFNEAFKYIDTNKWLKSKGLDIVSETGSLHSDINSVIQQLENSIKEINNLSNQPLFNDTHNSSSSSSTSIKGQDEESVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUQ2</accession>
    <name>A0A8J4PUQ2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TPR repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008738</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000569">
        <property type="protein sequence ID" value="KAF2069941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUQ2"/>
    <dbReference type="OrthoDB" id="433738at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="131"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="387" mass="45835" checksum="AA668E1759B4436B" modified="2022-05-25" version="1">MITSRYLKQLNKVDNHGFISNLFNKVNNNTIHQKHFLNINNSNTNKSNDIYQQQQQKQQSLLSFSLSSKYLSINNRYYSSSSSLVINQETLIEKQYKNLIEEAIIRFEADEMHHVMEPLDKAIKLLPNSSIHAYLLKADIYFFNGQIKESTPFYEKALLLDPTNVKLAYTLARASQLAYGDKRAIPSYQRLLQLDPKNEYALYRLFFSTLEDEYFNALKKIDSQWYNIALSIYIEKYPYSFHPTDIKDILYQVIRDEEEREEINNRSSSRGSDLPRYCIVRNSDGYPRLHRPFFNEVLYQSYETLAYAALSEYNYTDAFQLFKKCQEININSETIFYAGIIKMLRKEINTLELFDFLKSYIEHQKFRCDDKILQLRAQLQIEIEGFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUY5</accession>
    <name>A0A8J4PUY5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_004564</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000164">
        <property type="protein sequence ID" value="KAF2074122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUY5"/>
    <dbReference type="OrthoDB" id="504170at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14081">
        <property type="entry name" value="STKc_BRSK1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000777">
        <property type="entry name" value="CAMK family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF110">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="9"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="347"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="649"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="362"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="439"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="449"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="459"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="508"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="658"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2074122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="716" mass="79061" checksum="827381B539F1E77B" modified="2022-05-25" version="1">MEIHSVGPFIIGKTLGQGTTGKVKLGFHKDTGFKVGIKIINKELLSTKPSMRRKIEREIVLMKLIDHPNAMKMYEVYETSKYLFLILEYVEGGELFDYLVEKGGLESGEALFFFQQIITGLEYCHSRNICHRDLKPENLLLSGDKRIKIADFGMGSIVRKDALLHTSCGSPHYASPEVVSGVDYDGQKADVWSCGVILYALLTGKLPFDDENIRRLLNKVKNGAFSMPPYIHKDAQDLLTRMLIVDPSKRISIKEIKQHPWFTSNNNQIQKISPVEDIIVDPLPDVSAIDEEIFRSLMALGLGTLDEVKSSLVSNQKSTILIYYRLLEERKKFDNDVNKYGYKPKETRRASLSDMSLKKLFNGNSSSNSNNSNNANNTNNNNNSNNNSSKPNESNTANTVSSPHQQGSQEPSSSSSGSNSSSSSSGLLKQALQQHHHQQQQQIHQQQIPSISLTVSTDSNNSNNNSNSSSSSSSPMSTPSSSPSSSSVSSSPQISAIKQQENKYQRRGSMTATTNPATSPTMSHRGKTSSPIEITNKVRKLKISDSNASPANSPIIGSSPKKSWFSYFFSKNSNSNNNNNNNNNNNVVIETKIDVNLIYVNLEKTFRKYGFDWKYQNNTHCIVKTHQLNQDGTPQFECEIEIKAISSPVSRPSPLQLSANSQSTTTTTTTTTTTAATSTGVPSSSSDSTIRLSIIRKSGSANKFSNFTNSLEQLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVD9</accession>
    <name>A0A8J4PVD9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">LIM-type zinc finger-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_003036</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000090">
        <property type="protein sequence ID" value="KAF2075663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVD9"/>
    <dbReference type="OrthoDB" id="1840988at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08368">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="5"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="375"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="276"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="276"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="293"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2075663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="646" mass="71297" checksum="3447CE7BD68BEF63" modified="2022-05-25" version="1">MSTPDNCAVCSNAIKSSRVRANGRVFHEDCFICSSCGLNLDSFFFKDGKLYCDPCETKLYAKICDKCSLPIQTTVVNARGKMFHSACFTCVTCVQEIKGGFFYVGGSFVCDSCDKLPKNSRPSPPSPTTTTVAATASVTTSASSPVSIALSNSNNSTPPIVLTQPLGLNRNSITIGNTEIQVPTSTSTPTTNIIYVVNNTPDRVSGDIPPGVLPVQPHQPTSTDIQQNQILLEQQKALQQLQEQQLQLQQQLQQQMEQQKLQQQIIEQQHNLQKLQIQSSSQTTTPNASPTISRSTNSLSPSPSPSTTSTSTSVLSPSKLNISANDLLPPIEKYVPKIQGKTLPELLKNQSFDEILSDVLEKKMNVYNEIKKDEVEFGELMAAGASGKVYRGKYKGRDVAIKVYSTDNFCFSIEEFNREVSIMSLLDHECFTTFYGANKENPKYLFHISEMVKGGCLRDVLLNKDVALSYAQQLSIAVDVAHAMKYLHSLGVIHRDLKSGNVLITDDLRAKVIDFGTSRAIDLSKQMTLNLGTSCWMAPEVFRNEPYTESCDVYSFGIVLWEIYCRADPYDGVNSWSIPVMVCKGERPVIPADCPTEYAKMIKACWSDKAKKRPKFKEIYSTLNKLLGGLTLGKTKKSSSVGLFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVP5</accession>
    <name>A0A8J4PVP5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_004954</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000185">
        <property type="protein sequence ID" value="KAF2073752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVP5"/>
    <dbReference type="OrthoDB" id="15567at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08368">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09334">
        <property type="entry name" value="LIM4_PINCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000154">
        <property type="entry name" value="LIM and senescent cell antigen-like-containing domain protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000009">
        <property type="entry name" value="Paxillin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017351">
        <property type="entry name" value="PINCH-1-4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24210">
        <property type="entry name" value="LIM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24210:SF0">
        <property type="entry name" value="LIM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="181"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="239"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="304"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="364"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="29"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="88"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="486"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="101"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="489"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2073752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="527" mass="58092" checksum="70DD17C7C9D4FA47" modified="2022-05-25" version="1">MATNTNTSAVLAQMDDLMAELGLLGDQQPVPAAMPATTTTTTTTTTTTTTTSTSGSNTPPLMFKDPMVSSGPGLAPKNNMSTGPGLLSFGPGASLAQNNNGPMSSGPGLMSSGPGLRPANQYSPPLQSQQQQIHLTPQNHLTPQNHQQPIMPHLTDGINTLAPIQLAAQTPEGRLIKATGPTCGACGDMIIGMATNALGKSYHPEHFVCSYCKLPFRNNFIEHENKLYCEDDYLELFSPRCHACQKAIEDTCITAVGNKYHPDCFVCSGCGDKLRGKPYKEEDGEVYCNTCKVARQKRLAANAQICAKCKLPITGEYMLLQGQPVHSEHYRCEECGCEFKSGKTCHEYEGRLYCYEDYQKQIRNTCGECGKPIVGRSVTALGKVWHPEHFKCTTCQVPFAGSAFREHGGKPYCESHYHQYFGRMCFKCTKPVVDKGVEVFGKIYHREHFTCTGCENILGKEIMDWDGKPLCFKCYDALPKEVKKRIKQKKEGDKKAEKERDRINKKEAKEKIKEKKAAEKEAKKAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWN1</accession>
    <name>A0A8J4PWN1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TPR repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004589</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000165">
        <property type="protein sequence ID" value="KAF2074104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWN1"/>
    <dbReference type="OrthoDB" id="10249246at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099518">
        <property type="term" value="P:vesicle cytoskeletal trafficking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039494">
        <property type="entry name" value="F8A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16797:SF4">
        <property type="entry name" value="40-KDA HUNTINGTIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16797">
        <property type="entry name" value="FACTOR VIII-ASSOCIATED GENE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14938">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="696"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="371"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="371"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="939" mass="109300" checksum="3FABDA8D0AE0597E" modified="2022-05-25" version="1">MTNQNHHQQQLKRKLNQEIEIEIKNNKKQIINTDSQAQQQLYNLPKTLIKQILNQYIVNYAKIYYIENSHCFDRHFDFYVLSHAINHISCLCKDFYYNIVPSLQYPSFYLRSALEFKHCRLLIENRKFYFSLIDMRFYHPDINFNNLNFIILNLLNNNKDSNNNNDQLITSTTTDEDIKDITNLLVNQSYQIKSTFNLNEINSRLNYLKNILNNNNNNNFIKKIESNNINNNSKESFLNNILPNLIQQYEVQEFYFKNDSVSKDYQPFSITSDSKLIFNSLSTIKIDQSINQSTFQDILISSNNLENLELKWTRVNLFIENLINHPTITNLIVYEKSFPNNQLLNNLANYLSKNSILSSLTYSYEDDFIDESDSDCSDNSDSEDNEKDSNDDKIENIEIINNSKLKNLNILYDGKFQFNLMDSLLGKWRQQSNIQSIVCGEIINYQSFKMHSNLQSLNIKTFSLDYHTSNLREYDYLKNLKSLLFDDMDFRNSYNLNLIDYLFNNIKSLSCPCDLYDHDNLNFIFNLPTQIQHLSLENQTPTNLKSSQLIQLIDHFPSLSTFSLQFIHIDFDVPIKDFALFLSTKLKKKKFPMRKPNYSEAIDQFTQVMNSFKRDNNLMYAAFCCLSIGRCEQALKSSSSAEASCYIDSGYLIWENEIQNSEADIISFEENIPEAINCYLLAIKIYHNYKRFSIMSTLYYEMALILKKLNKLEEAAEYFQKAAEIQRTESPISSINSLKEATYCKIMEWDYKGACNFLDLIVTIATETIQPFESRICQLSITSHFDEYNNSHSNQNNLNSQNLNNNINNLNNYSSSSSSSSFKLNSSNTLPSISISLFPFLLIEARVSLFLLYILQESFSKSHVQLSKLVNDINDHDGNNGGGASLAEDTLALLNNLLVACERKEINGLPAIQRELWSSLNDMQNDILQRVWCSNKMLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWY9</accession>
    <name>A0A8J4PWY9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosducin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003449</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosducin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000108">
        <property type="protein sequence ID" value="KAF2075231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWY9"/>
    <dbReference type="OrthoDB" id="45518at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02988">
        <property type="entry name" value="Phd_like_VIAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051498">
        <property type="entry name" value="Phosducin-like_chap/apop_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024253">
        <property type="entry name" value="Phosducin_thioredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45809">
        <property type="entry name" value="VIRAL IAP-ASSOCIATED FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45809:SF3">
        <property type="entry name" value="VIRAL IAP-ASSOCIATED FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02114">
        <property type="entry name" value="Phosducin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosducin" evidence="3">
        <location>
            <begin position="52"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="206"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="234" mass="26825" checksum="23456B7D38022E11" modified="2022-05-25" version="1">MSGRTEWEDIQIRLGNMKAPPKELTEDELFDLIQEAAVLASEAEKREKLENASLEDLREMEDDEDEKVLEELRKRRIAQMKLEKELNKFGELYHISEPSYKSEVTETKGIFVVVHLFKSGIPQCQLINEYLGQLAKKFKATKFVKIRSEEAIHNYPDKNLPTVLIYYSGDIVGQIITLAQTGGDATTMKDIEWQLAKFGAVKTDMQEDPKLTAKKKKSSRGKYDSDESDDSDRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX90</accession>
    <name>A0A8J4PX90_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MACPF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_005006</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000189">
        <property type="protein sequence ID" value="KAF2073689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX90"/>
    <dbReference type="OrthoDB" id="21110at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022829">
        <property type="term" value="F:wide pore channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052784">
        <property type="entry name" value="Perforin-1_pore-forming"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46096">
        <property type="entry name" value="PERFORIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46096:SF3">
        <property type="entry name" value="PERFORIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035162483" description="MACPF domain-containing protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="domain" description="PA14" evidence="5">
        <location>
            <begin position="302"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="MACPF" evidence="4">
        <location>
            <begin position="505"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="744"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="759"/>
            <end position="769"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2073689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1125" mass="125270" checksum="1396DFBF7F456D16" modified="2022-05-25" version="1">MNYLVYSLSLLLLLVFYPSHGHLGQSNFVNYYLDSTSTCVSNCGGSEDQAFSNFKEAISSISNNKDSIIPYLNVNQGDYFGIENNGIKITIDINIVSTKGSLETTIDCQGRGSGFVVEGPISFTLIGFTIKGCNAGKGAAIHSSNLITELEDIVFIENTARVGSAIYSSSKIFNMDSCMFYLNKGGFSVHFTNVSSDISNSQFNMNANDVYCEGGNIISSESQFGSTCNSCSILGIKFGSDICGSSKTSNPCNFDGVCQKWIENFNNCPADCPFRTLCKIDGVCHSKNGENAINCPSDCDSDSHPGWKLESFDFQVEKPSESVAIFEDSPKKVDSVFLPFCNVRSFMAKKYDPVSGRISSNVIVSKDGDYYFRLATENIGAIVFVNGRVLFDTFSFHLKDFSSERKLILVQDAVTFIEVVFTSHSSNQRNIALDWRHESKSEYQPIPSFFTSLSEKFYCGDGKCNEKIPENCLVDCHSHIEKICPGQSPPAKLQDFYGDTTNDITENLLNNQYIFSLPGISFMSHGLDLRTGETNTAPLFELSYCDNTSFSIIQDPYRGLVYSVPSGLFAQVAPKCTMDSTTKTYSNSKHMASEESKDMSLDVSASGGGGTAFVQVSVSASLSLSKSTKSASDTESKKDGSISKTTISCETTKVHIVEHRFHPKFIQEVSNSFVDGDPNNSNLNLQKVIKKYGSTYLKFATFGGKIEVITVVDHSFSSIKSSSEVANSMEMSVSAQVSSKVLSGSVSSTQGRDSKTDTAEQNTFEDNSERSTMQIYGGQPGSYGKEDPNSFATWAKTVDLVPYPIDFKVGYVADILPDSWFFKDGHSVKMAWLLNEWRLFKDYLKDREFKYIKYEDHLEEEQLKRSHTIYHMTFKITESFDLEITNSKSKVFFFKNVKKFGATLQENMIIDYDDTKGVSCMKFIALGNYPKNKHGITKVGDTYYHTPVDNFQAYNIFTSRYYNFTKSVDSVTYQIEPAPFGKVILNFNKLTTHSNNQAANVDLMLYGTLGKIKYSINMLKPPLMPIELDAGYYLGKILGISFKYFTDPNASFKDYIIRFESLIVIQGCPEQISSLCVPKNNYYNSQTGFVNSFEAWRKPQGENLITIPYSETQQSVWVRIEPLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXK8</accession>
    <name>A0A8J4PXK8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002799</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000081">
        <property type="protein sequence ID" value="KAF2075908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXK8"/>
    <dbReference type="OrthoDB" id="16423at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="17"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="196"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="287"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="6">
        <location>
            <begin position="19"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="313" mass="34778" checksum="327731A84CDE0E61" modified="2022-05-25" version="1">MIESIIGLANRSNTNHVAAFSLAFWFVLNISTLILNKFIFSTLLFSYPITLTAIHMLICSIGSILVLRVYKLVPLIEINYSEQFSKILLLALLFCSNIVFGNVSLRWVPVSFMQTIKSSVPLFTIVIQILFFKKTFSTKTYLSMIPIVGGVCLASVSEVNFNQAGFLAALIASIITALLAIASGMILDKQMNAVNLLYYMTPPSFIMLLPLAFFTEFQSIKTDWVYYGEAQPALILILSGVIAFLLNIFTFLVIKFTSALTYTVSGNLKVVLSITISVLIFKNEVNFLNGIGCCVAIIGVIWYSQIRYEAGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PY15</accession>
    <name>A0A8J4PY15_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002464</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000068">
        <property type="protein sequence ID" value="KAF2076223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PY15"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="83" mass="8803" checksum="6CDA7B36A4E32B7E" modified="2022-05-25" version="1">MILNTLTSLANPTQSISKAVIKGSNGGFGSNGSHRLSNAIRSPAGSSISPTTNVNINGKKMEGNHFKDDYCSYDFNLNNPSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYB3</accession>
    <name>A0A8J4PYB3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TFIIS N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_001331</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IWS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000031">
        <property type="protein sequence ID" value="KAF2077328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYB3"/>
    <dbReference type="OrthoDB" id="21124at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051037">
        <property type="entry name" value="RNAPII_TF_IWS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46010">
        <property type="entry name" value="PROTEIN IWS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46010:SF1">
        <property type="entry name" value="PROTEIN IWS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="4">
        <location>
            <begin position="401"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="62"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="128"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="193"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="270"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2077328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="583" mass="66817" checksum="674FCCB0A0921422" modified="2022-05-25" version="1">METTTTTTTSESVQESMIHNNGVTETPMEESVAPTAQTTETPVIDNTEKPTIEEYSEEPSNTTSTTTTTTTTTTATKENKDEDEDEQESTVVRKKKKNVIEEDDEEGDGGVQEGKEEETVGAEVETTAGEEEGGEMDTDDKKKKKSSKRDKKKREKKEKKKKSKSSGDEQEQEEQEGEEEKEETNTESEPKSLKRLRRSSKKKQKKDAEEEEEDENKELFGEDDDDEQTTFVEEKKKKKKDDDQDYEDEDQHNEDEDGEEEEENSETESKKKKKSSKKKDKKDKKKREKKEKKEKKKKSKRSHDDDEEGGVVAQQEESNPLDEFLNANKKKKKKNDEDVRASCEDMIHNLLNKMNWAFEQDNQANKEKQPALHKLMLLPEIEETLSKSILYKSLVERDIYGIINDWLSPLPDGSQPNLKIKEVLLKALFKLPTEKHRIRSCGIGKIIMSIKNNAKETPSIKKLAYDVIDKWTRPQVSLSKHYDDMQVDLKSLQHQSRDIDPENPDSILSRKKDDYLPAHVKHASGCARVSLDYVIRPVGIVNEAINSPTRTINRIDKHIEKLKRPTIKGSSRANKISVEGRGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYG2</accession>
    <name>A0A8J4PYG2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003435</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DENND6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000107">
        <property type="protein sequence ID" value="KAF2075259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYG2"/>
    <dbReference type="OrthoDB" id="10265409at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018307">
        <property type="entry name" value="ABL9/DENND6_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024224">
        <property type="entry name" value="DENND6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13677">
        <property type="entry name" value="LD41638P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13677:SF0">
        <property type="entry name" value="LD41638P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09794">
        <property type="entry name" value="Avl9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="3">
        <location>
            <begin position="91"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="617" mass="71365" checksum="9BCED073420A7212" modified="2022-05-25" version="1">MDIIEGLDPLSIIAKEEEDKKQQILLLQKQEEQQQQEKRRNDSKTKHKELEEEEKKLESLTKLHLHQNAGNITNSGDTLELKQHMRNKWMNHFCVINFDLEIGQILDYSFPNATLKDEESTNLCFLSFPDSNSHLQGDIIYSFKLKQTLSNGSTTFQYGYVFFRQEKDASISRGYLQKSVVLLSDESFVGLFKKVIEIVGPLYFDHGNTLLEVAYQNILSWPELKLGQTYELPILGYILTFHVPHTRGTPHIIDPSIKQHTFSTPWTEMKLVSNLKSIDLHNCFKGFTSKLWMLWEMVLLGQPILVISQTPPMCSDSVLALVSLISPLRYCGDYRPYFTIHDTDFHKYTSLQSNNQIPTSIIGVTNPFFLKAFGHWPNILTVGNASSRLGSFKKIRSSLPNIMSRDLLSKHVLDNKEKLVSDYKPFTVPDKNVLKKISETVDDTVNNELLRTHFLQLTHTFLIPLERYFSSLMPLAKTISIFQRPPRLKSFDKEEFINRISESDEKLIVDSKSKEIEMYKLFLDSVNFKQWLDDKRAEAIKHLNILYRKAILEADILSLLKSKNSSSTATDLYKRIEDQLILEENLFQTSDEIKQKFKSHLLIIKEYLAVNKTTPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYY9</accession>
    <name>A0A8J4PYY9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Checkpoint protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_000899</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the HUS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000019">
        <property type="protein sequence ID" value="KAF2077778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYY9"/>
    <dbReference type="OrthoDB" id="337750at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030896">
        <property type="term" value="C:checkpoint clamp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044778">
        <property type="term" value="P:meiotic DNA integrity checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016580">
        <property type="entry name" value="HUS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007150">
        <property type="entry name" value="HUS1/Mec3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12900:SF0">
        <property type="entry name" value="CHECKPOINT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12900">
        <property type="entry name" value="MITOTIC AND DNA DAMAGE CHECKPOINT PROTEIN HUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04005">
        <property type="entry name" value="Hus1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011312">
        <property type="entry name" value="Cell_cycle_HUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR011312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2077778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="274" mass="30851" checksum="214D46EDBBD3307D" modified="2022-05-25" version="1">MKFSAKLVKAKVLLKIILNIQKIPGDCVVHITQKKVAFIIRSDLDEGGSQVWCEIENSNFFDELNIQSLSDNEIQFQLEIEHLKRVLQSATTSMNEITVKLAKRGIPYLKFIIQSTQQSVILIQEIPIVLLTQQQMAQINEPQLPDPDVNIFLPNLKTLQNIIDKMKNISDELCITISNNSELKLAVETNAGSISTFYQGLTHATLGGKDKTQSQITRSSAVVDIKKFQKVLVLHSLSPDDVVCCIYESSLLIHAMLHNHKVVVTYYLPIIIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZK2</accession>
    <name>A0A8J4PZK2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003522</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000111">
        <property type="protein sequence ID" value="KAF2075174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZK2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2075174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="728" mass="85770" checksum="B7D61531F9A9840B" modified="2022-05-25" version="1">MVKPSTTNNSNNIDVYYNLVFRNKSLRYYIFEKVKDNLKIGYNYYQLPLGVVIKAKRYDYLFDRIDRYIQWRSDPSISDAYLELDELDIDQLVKVPDIIGYDRFMILFNDFRHVFNKYISAEKIASCSDFKIFLHLISVYSLHLDRLNRDSYLFLSHCTNVDICKYIVEPKRDTYLGGYWVERFNVEFLFTKSLEFFNYYVNLLFSSQSTRQDNYFLTVHKSSVIKMLNDGVVSEDFVREYCNILVSFPNIKPLEFSNFNFPYLLKLNLNIRECFKRFEIMQGYNVFSRISADSYHSCFRYFSVIFGVPAFFKSEDCMNFTLGMFGNRYLGLDGRIVMKSLNHLISTGDLEGVKLLYKLCPLLSHPLRPTFTNHISLPIFKFLIEDWRCIDEASFTPYSFASLNCGLDSLEYLAIKFPDLRPSIAQHFALIIVKCPPALVGDLEKIHSLASMVPKLKLTIDLLLEPKRLDFLQMVFANPQYFELNDNALDARPHLIHKIFLSQSKQLLEFALSVPVLRKHFQRYSGQYSYHNNYSQIQKYIHCINDQYALGIKFTNINTQSTTWPLLIKQQKYNTTLHYLYSNGFQIRDICKVFSNLIKKGMEKTNYVYLFDRIFNSKYFINDQEKKKSILFSIVFISLAHDVYPVIRDYLFAKSIFNRPSGDEVIEQQQIKCNFNHIFLDDKAITNVTNTIKTLGIQDQNFLCNIGFIDQIHIHNIKQSNLHDLFDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZQ7</accession>
    <name>A0A8J4PZQ7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002701</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000077">
        <property type="protein sequence ID" value="KAF2075994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZQ7"/>
    <dbReference type="OrthoDB" id="21001at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="191"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="326" mass="38670" checksum="832703B62BDA759D" modified="2022-05-25" version="1">MDYIYQDDVLPIFLKFNTTPVEAREDYEKKIVNVKKPNYLNGSKKKELAERYLEYIENQTDKFLIEESNFNFSVIPVMYLDQPPSSMPEIDRNLKTVRKYMDDLSFTNNNINNNKNHNNNNNNNNSIPNQHNTMIYKRLIERLIDTVYKMIEIFKKESLYILYHDISKLEDQYSIDIYLQHPLIKVQNSISRSNNNQNNNSNNQNNNNKSASTTLISTMEIKNDFNFKEFKNSCLKVLNRLERKQTNLDLQCYKEIIKDISHLYTMQHYDKVFYSMDHSSISPDDPAVGVTGLVVNRLHRLENELKTIQQHWNGTMLNSHNLVYKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q078</accession>
    <name>A0A8J4Q078_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Membrane bound O-acyl transferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_001987</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000051">
        <property type="protein sequence ID" value="KAF2076730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q078"/>
    <dbReference type="OrthoDB" id="286734at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030258">
        <property type="term" value="P:lipid modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049941">
        <property type="entry name" value="LPLAT_7/PORCN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906:SF4">
        <property type="entry name" value="LYSOPHOSPHOLIPID ACYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906">
        <property type="entry name" value="PORCUPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="358"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="404"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="439"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2076730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="470" mass="54043" checksum="B7E6DD1CEF78E56F" modified="2022-05-25" version="1">MDQILDIIGLFPVDQIKTLACLLTSYPFALIVKRLPSANAKHILNIILGVAYCSFCLGQWSWIHSFASSLVVYGLLMVLPKRFAHLVVFGFCMLYLSISHIYRMMNDYMGWTLDYTGPQMILTLKLTSFAWNLYDGSRPQDQLSGDQKKRSIKKLPSLLEYFGFVYFFPTFLAGPTIEITDYLNFTSGAMFNDERLKGKAPASGFPAFKTFIRAIICFVFVFISGDYAPYYLATDAFRSYTGIFEKIVRVHIHVALSRFKYYFGWYMGEGSAILSGIGFNGFDDKGDAKWDRITNVYPLRVEFASNIREVSTYWNIGTSDWLKRYIYLRLTPPGAKPTFFATFAVYSTSAIWHGFYPGYYVFFIMSTFLTEVAKDMRRKLRPYFVKTIETPTGPQEKGTVLKPLYDIVGLMCTEWFLSFFGTSFLLLSIESTLQIWETFYYLPPIILLLSFFILRYIVPTPRSGSKSKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0U1</accession>
    <name>A0A8J4Q0U1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001614</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000039">
        <property type="protein sequence ID" value="KAF2077105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0U1"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22967">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22967:SF91">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DDB_G0280111-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="16"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="309"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="745"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="867"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1037"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="309"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="347"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="368"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="407"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="444"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="745"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="777"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="790"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="815"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="884"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="902"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="915"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="939"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1046"/>
            <end position="1055"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1062" mass="119581" checksum="FA2B2119993A2C07" modified="2022-05-25" version="1">MGNHQGKTLKIGSYHLNFVKQIAEGGFSYVFLVKDSNTSKLFALKRILIRDEDEYREVKHEISIMKKLTKHKNIVKFIDFSKTSDKNNTEVFILMEYCSGGHLVELMQKRIQNKFSEHEILKIYSDICESVAYMHSQSPPIIHRDLKVENVLLEEESGLYKLCDFGSATQDITHLKNKVDMQRAEDDISRHTTIQYRAPEIVDLYRCNVITEKIDIWALGILLYKLLFYTTPFEDSGSLGILNANYTFPSNHPYSSNLINLIKLLLSPDPKDRPTIFELTNQICLLRSLPPLFPSHKSDILTIFIQQQQSSPSSSHSPSQVSPTTVVNSNSISPNQSKPFPKPLPKVSSNSNVSISNNHNNSNNHSNQDGSLNIPVSHSRSSSKEKYEILINDSNSSINNGSGGSDSGSDGSLVSSFGSMSFGAPPNSSPSPQPKRRVQKASAPLQPLQQQPFPNDDEFSNFDTSSIEEITMIINNLTSQDFNSLDTSLLIKLKTLSNQNHNIKSLMGIIVKRPLIEPLICFKSLILIQILLSEGLPSFKKDCYESKDLFNNLFLGWLKQKDRYLILGDLLGQYSGLIYKLVLFHHKYYLVDGLFGFDEMKWGLPDRLESNHPISTATITNLYEIMDQIFLVQQCLIDMSNSNTSPPTTTATTPNINLPTTLLSQCVNILNSSSFAIYCFISGCLESLCKLFSDVDFQLQNSISRFQAMYQKLRDNYLNLNTLPTFSDLFFPTLPSNPPTFIINSNKNNSNYHSNSNNSNNNNNNVDFGDNPFANEPSFNPFNTPSTAINLNNNSLRNSRSVSGEFKEPSPVISPRNSQYISPSGGSSAIQSPSMPSKKLPPRFPHSASLEDARLYTLILTPTPSPPLSPSFGRLSHSNTSVPNNNQNQNNINNNNNNNNNPTPFDNFNLNVYQPTPPSQQMPPPPSIHVNKQLPPHPQTQQQQQYIPQSTNPFDNPIPPPLNQHQFAVPQQPAPSIQKSSGSLMPPPLSKLPPTRNHHRRSQSNTTDELRRRNLLQQQLDQNRDFLNHQRLVNKNSRLTKGNENDYYDSDEFDYDEKGSLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0Z4</accession>
    <name>A0A8J4Q0Z4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002853</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000084">
        <property type="protein sequence ID" value="KAF2075819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0Z4"/>
    <dbReference type="OrthoDB" id="20747at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.980.20">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="522"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="733"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="776"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="838"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="199"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="247"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="529"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="552"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="567"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="733"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="777"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="838"/>
            <end position="857"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="892" mass="101424" checksum="8535AA88B5B1B534" modified="2022-05-25" version="1">MENRYDNNNFIQQQQQQQQQQQQKQSRKNQQHKRNLEININSPPAPLPPTMTYRRDYYRPAEEGINIQQQQQQQQTYQQPQPQQYQIHSQPSSPTSNPTVLPQYHPQPIYVVPPNQYYSPPHSPTGHHPYHPITRSNSYSNIHYSRHPSPHQIPHSQGGYPHFIDSPRYKYHVPPNTIAIEDYNSYYNHLQSTLPPQVQIQPNRNNSPQIQPPQPQQIQVSNLPPHPYYTHSNKGISFSTSALPDSLINNSSNNNNLNNSSSPVQNFNSNQQQIPIGNKSPTLEKDIINVDENNSIHIINNIYKNKNNSNFNNNSQNSNDDLNNSNGSINNCNNSNLNNSNNSLNNSNSSISSNTNNINNSNSNNNSNQNNPNNSNNNSNTNNNNNNNNSSNNNNNNNNNPNAYQCSIYTKCCLCNRGSPNILKAQTWANIMRVVFFCLKTSYPSKDFFNLKHDVYGFMSSHWDVLCFEKKKSDNWHKQIQDILSHSKELFESGMEQYQQNGFWRLKDMSDPWTIIKHFSSRKRNGSLNNNNQNNQNNNQNNQNNNQNNNNRISQSNSYDNLIFDSNNNNNQNNNNNNYNNNSNNNLLNLNFNNVSPRSNNNSPRNVSPRLISSSANSSRSNSPRVVELSTQQHQQITSPSPLNGTLRVAPALNRINVTIPNSDSSISSPRINEVEKLFSISSITDTEENFLTERYSKKTRNENIDHSTLQLVVENENIDNNSFVTNEIVENSNINNNNNNNNSNNNNNNKSNSNKKYHIDQEKWNSLLKGFMGFNTLPNKDSPHSPQSPVSLPSPHSPNLTHLQNHYKKRSFSDSSFSSPPISASSLNSIVAVTNSESNSSLGSPITIETNSSRPSTPKGEVNVINKSNISNNNNNYKGYVNFVSPRDNIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q154</accession>
    <name>A0A8J4Q154_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000848</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000017">
        <property type="protein sequence ID" value="KAF2077886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q154"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="181" mass="22814" checksum="F41DA5E1FB3B16C2" modified="2022-05-25" version="1">MELIEFGCLDSIELINCYLNHGFKLTENVFTSKYEILEFLYNNHYLHHLEFNQNYILRNPRDLRKIKFFYEKYPSYFKDFKPWKEGDFRISREPSICKFFFERGLFDKLTPDEYQFLFFSEEWEKDVYLHLCKQNPSFKENWKFSYREEHSFRSSYIREWTICEKFSSEHWSFLREFFYFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q254</accession>
    <name>A0A8J4Q254_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000308</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000005">
        <property type="protein sequence ID" value="KAF2078441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q254"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2078441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="173" mass="19894" checksum="89A898699D32AC29" modified="2022-05-25" version="1">MENLLPEFEAFALIHPTCLATTKHLLQQREYLSKQPLIFEIDIDGWDLNLMDNDYESEQELLSISTNFKPIIYENNNINNNINLNNEINNINNNSHENSNNINNINNNNNDNGFKLIKPKAIHLSKSSNIVTVSIPKVKKDIKKSLKRNNLLSISKMMKNNFYSSSRTELSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2P5</accession>
    <name>A0A8J4Q2P5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001654</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000041">
        <property type="protein sequence ID" value="KAF2077022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2P5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="334" mass="38232" checksum="21FE3A8871526B19" modified="2022-05-25" version="1">MTDKKRERGTKHRDFDISAFQEWSTDLFEQWLQITFSQKIVSKLPLEQIKELHDIFLSLERDTLKDLGLALFEIQAIEKRVQEVKEAGKLREINNRYFSIPVIETQRKIVKIAHTQERTTLAFTSSIVPSANVTDIINEVDWIQVDKIPQKNADACKASLQVLLDCGVSLYTSLNEETKRLFIHPILAFTIQSINQENIKLFNGEKLALRLDTPLKNDPSLGIAYTVILVDDVIFIIVEETDSDFRKGLNQIKAAVHSIISSVKRSRKMNYIFGIVTTADRWQFFRFSQGFEIDLFVYLALIGNRDFDPSPLMSLVRGFLIQGAIQSGVLPPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2T9</accession>
    <name>A0A8J4Q2T9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_000450</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000008">
        <property type="protein sequence ID" value="KAF2078260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2T9"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001435">
        <property type="entry name" value="Probable myosin light chain kinase DDB_G0275057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="53"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2078260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="342" mass="38395" checksum="182FB7500005B248" modified="2022-05-25" version="1">MGCFNSKEAKPRDEQTKVQPNPNETTAKTAEVRAEEKISSAQDISIGNVEDHYIVGKELGRGAFSVVREGTKKGSSEKVALKYIEKKFVKKKHIEQLRREIDIMKKVSHQNVLALKEIFESDSHLTLVMELVTGGELFYKIVERGSFTEKDARNVVRQVCAGVEYLHSQGIAHRDLKPENLLCSGEGDDMIIKIADFGLSKIFGAGEALETSCGTPDYVAPEVLTGGSYDNAVDMWSIGVITYILLCGFPPFYASSQNLLFEKILTADYDFPEPEWTHVSDNAKNFIRNLIVKDPDQRHTAKKCLEDAWITGSEVNQSDLHSHFAEKMKKYNDQRRNAQPAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3C3</accession>
    <name>A0A8J4Q3C3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_001370</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Rho GTPase-activating protein involved in the signal transduction pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000032">
        <property type="protein sequence ID" value="KAF2077304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3C3"/>
    <dbReference type="OrthoDB" id="79452at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050729">
        <property type="entry name" value="Rho-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176:SF127">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN GACJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176">
        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="6">
        <location>
            <begin position="157"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="66"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="379"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="437"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="72"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="91"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="379"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="400"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="486"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="506"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="529"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="541"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="630"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="648"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="676"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="705"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="738"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="761"/>
            <end position="771"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2077304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="808" mass="89632" checksum="0CAEECD2D87B1AAE" modified="2022-05-25" version="1">MSTNNTDQQQQQQESSSIKKKIVDFLTQFIKKRPPPEELRDKNILVAPSTVAPRIQSTRYELDQQLNHRQDSNSNSNSSNSNNSNNTKPTIKKENSFKDKDRWASTTGGMNDKPHRKETFKIAWDCIRNLDFTFSKFKYGKTVVHRLKFPQFGLSPEELQNLYPDQPHGIPLVLTKGFEYLSKYLETEGLFRVSGSKKDISLLKFRVEDGDLDFSTELNPYSICGLISTFFKELPEPLIPLDCYQEAIDSTKIDNREKRIQALKTLVLDLPPANLCILRRFLHFLLSIEKKKDINKMNSDNLGIIFGPTLLKDPEFADITTSLGNLKYQSLVIKYMLDHYGDIFQNSVVDKAYRKSIIPKEQIDTSSLNYLDIAEDSSSTRTSVSSNSSGGQRTSRPSRPGSNRKTLLLSPRTSGSSSALCYSTLTRPQSRVLLENNFEISPSPPPKTTQYSTSPLPPKPPKPPMRKIFKPQTNPNYQTYPLPMSKQPPLPPSAPPRSILKNTSMAPPPSKPQSKPPPQKLSNPISFQTPSSTTTTSSPISPSPPKDYSQTPPPPRAKPPPPKRTFTNYTIVDVELDSNNNSTKTTFEKPPNKPPPVPVELKKNPTPPATPPLSSSPTPFLTKKKVSIVEPSTTATTPPSLINKPIASKSSPPMVSASTKSQSMSSIKDYQEKLNSEQQTKASQPISVSYFSAKSKPLVKPTIASSSSTTTTTTTTTTNSRDDLNINSDKPSVAIPSKSRSALNLSTKQPGSPTNTPPSESPMSSSPPTTTHIAKFNHLTPSTLTVSQKIALNEKKIQVQTTKQQQFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3E4</accession>
    <name>A0A8J4Q3E4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000595</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000011">
        <property type="protein sequence ID" value="KAF2078124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3E4"/>
    <dbReference type="OrthoDB" id="17882at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="32"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="159"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="372"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="296"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="41"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="376"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="702" mass="78515" checksum="4353BC508FCE4D3F" modified="2022-05-25" version="1">MDDSVLFDNFIDSANLESNLINIKIHSPSLPPYIKPEPQDTPSTTFFNNNSHNNNNSNNNNSSSNNSNPTTTTSTNTNNFNTSFLNNTNGINNLGNSLQNINLPALLSLPTQQQILSSIPTPTDIQNSATESIQTLLSTSSFNPNSLYSYYSSSTGQNNNGADSGLQNSISTDSGSNTQFCETSPSMMINSVHHQFINQQLQQQQHQHHNQIQNQQLNNYQQQQQNRSIYIHSNNQQQQNNNVVISTPDQQQTQNVQQQNHIPNHLHLQNHHLQPQLQIPQQNQNIQQQNIQQQNIQQQIQQQFQLQQQQQQLQQQQQQQQQQHLQLQQQQQQQQQNSQQNSQQNTISIHNPLFHELISDNIAPYLLNSGSNSTTPSLSDISSPMSSPKIIMTNPQTTTTTTTTTTNKNTTSLINNSTTTSSSSTTIDPLATKQDSTQGFLSATQIPVLINGELSIVIEQQPPSDVRTRTPNDKRVFSTVIRVIGDLEKNHVGGVVIQLAYANSANSERPSQFILGGNKVGTIGKDGKVDFEGLTMTEASTKHRENEFCLEYILISTDNRILSTSNGPYIKRSRPFYAYSNQKVLSRRRNVTLRTLSSTRGTTLGGEQMHVVGSPFIRCSSLKCIIHTPQGDVPASNIELYSESVLFFTLPPYPIPANFSQPEGTELPVQIVVTNDGRNYSNPLSFTYIYEGTGQKRLRSRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3S7</accession>
    <name>A0A8J4Q3S7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CHCH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000009">
        <property type="protein sequence ID" value="KAF2078220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3S7"/>
    <dbReference type="OrthoDB" id="1106148at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055304">
        <property type="entry name" value="CHCHD2/10-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13523:SF2">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13523">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 2/NUR77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="159" mass="16299" checksum="6FB4EB3B31537B8C" modified="2022-05-25" version="1">MARRGASSSMSRKPAVASKPAAAPAPAKTPAKATTAATPATATPAAATPQPVVVQGPGMLGTIAGTMMGAAAGHVIGHGIVGAASSLFGGDKEQQVEQTTMSNDFNNTTTAAQDPSTACNPLYKSFMTCLEKNQNDITSCQWVYDSFLECKKNPTPAYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4W0</accession>
    <name>A0A8J4Q4W0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CP-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000341</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000006">
        <property type="protein sequence ID" value="KAF2078357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4W0"/>
    <dbReference type="OrthoDB" id="1696305at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01855">
        <property type="entry name" value="YqeH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050896">
        <property type="entry name" value="Mito_lipid_metab_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048422">
        <property type="entry name" value="NOA1/YqeH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46434">
        <property type="entry name" value="GENETIC INTERACTOR OF PROHIBITINS 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46434:SF1">
        <property type="entry name" value="GENETIC INTERACTOR OF PROHIBITINS 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21516">
        <property type="entry name" value="YqeH-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="1">
        <location>
            <begin position="201"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="576" mass="65760" checksum="63A491F39044B408" modified="2022-05-25" version="1">MNRIISSISFIKPICKRNVTTLITNSKQLQLPSFSIKYSTTNSSNGSNNDENNVNATVIESESEKKLSSKQRNILKKMDQKTKQKQLKSQNAIDIAFEFDKKSLPRCSGCGTQLQTEKKNNLGYIPESVALRLLSKDNQTTNHHHHVDQNQKDEKDLKEYFNDFKSKFNKNDDEIQICQRCHLLKNHGKISSVNVPVENFRQQLEQLKHMNCVIIKVVDLMDFSGSFVEDFRDVVGNNPVILVANKLDLLPRDIKHQRVEEWIRTKAKEKGLLSIAHVKLLSSTNREGMQSFIIDLEKMRRGRDVFIVGCSNVGKSTFLNSLLEEYKNKVEIKREDTMLESSTPLNRKQLKDIDKMKHTQGPISKATTSVFPGTTLNLVSFPLWDNSNLFDTPGIDSPHQMIKLLNMEELKKLIPKEKVKPTVIHMSSGKTLFLGGLVRIDFEGPISTFTVYVSNKLPVHTTSLRKADDLLHNQHGKLLSPPILPESPTQQERDAHLDRFHFSQIQNFSLVPEKIDFEHTFVDLVISGVGWVSIKCISKKVTPLRLTVHTPSKIDVSIRRPPLIPKTNFFKHQNPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4Z1</accession>
    <name>A0A8J4Q4Z1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_004650</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000168">
        <property type="protein sequence ID" value="KAF2074036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4Z1"/>
    <dbReference type="OrthoDB" id="449052at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF34">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="18"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2074036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="326" mass="36889" checksum="C007A6FE5FED7CE2" modified="2022-05-25" version="1">MTDFVEFLNTTRVGDFLKSKDMIIVESSSTLDQLLNTFYQFSIFSAPVVDQQKVVGVVDMLDVVNFIIEFSGLKIQSNLSLLETNPQKFTSILGTGGNLFSESTVSTILNNRQNKLFVVRNVDPCMKLISIYNDIITKVFILDELNEIINLASQTDVLAIVAQNIHLLGDRKKFTVEQSGICTTFKKSFTSETRVINILKEFLDNNMIAAPIIDPKTSKLVANFSITNIKSLKKDNFDELMLPVLDYLRFQSIKEKKRNLSCLKENSFYPLTCYATDTLEYAIFKLVATKVHRLWLIDQDGKPSHMIGIDAILKNIALPSMDVEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q562</accession>
    <name>A0A8J4Q562_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamine amidotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000147</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000003">
        <property type="protein sequence ID" value="KAF2078522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q562"/>
    <dbReference type="OrthoDB" id="92161at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01741">
        <property type="entry name" value="GATase1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044992">
        <property type="entry name" value="ChyE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695:SF3">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE CLASS-I DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="1">
        <location>
            <begin position="30"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="263" mass="29714" checksum="EFD9B543C37BCB73" modified="2022-05-25" version="1">MSKIVDKTLRIGLLKCDTFIPEVRNKFGDIDVQFRNLLNQNTINANVDLNVFEIASENRFPDKKDITSNHYNGFIITGSRSSVNDDNEWTNTLKDYIRFFSNNNIKTVGICFGHQAIATALGGEVTMNDKGWMVSDHSFKIKDSLQSNNNYSKIHKSLSPKDDSTINIICSNKQIVSKIPQGFEIIGGSDICPNHSMIKDNQFLSFQGHPEYTPNLIKTIIQTRRGLIPDDVIEDGIKRADNSKIDQSTYANTILNFFLNKDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q5G8</accession>
    <name>A0A8J4Q5G8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Trafficking protein particle complex subunit 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_000055</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000001">
        <property type="protein sequence ID" value="KAF2078684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q5G8"/>
    <dbReference type="OrthoDB" id="203724at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990072">
        <property type="term" value="C:TRAPPIII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058541">
        <property type="entry name" value="Ig_TPPC8_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058538">
        <property type="entry name" value="Ig_TPPC8_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058540">
        <property type="entry name" value="Ig_TPPC8_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057651">
        <property type="entry name" value="Ig_TPPC8_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024420">
        <property type="entry name" value="TRAPP_III_complex_Trs85"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12975:SF6">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12975">
        <property type="entry name" value="TRANSPORT PROTEIN TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24545">
        <property type="entry name" value="Ig_TPPC8_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24544">
        <property type="entry name" value="Ig_TPPC8_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24546">
        <property type="entry name" value="Ig_TPPC8_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24542">
        <property type="entry name" value="Ig_TPPC8_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12739">
        <property type="entry name" value="TRAPPC-Trs85"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPPC8 first Ig-like" evidence="3">
        <location>
            <begin position="581"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="domain" description="TPPC8 second Ig-like" evidence="2">
        <location>
            <begin position="789"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="domain" description="TPPC8 third Ig-like" evidence="4">
        <location>
            <begin position="891"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="domain" description="TPPC8 C-terminal Ig-like" evidence="1">
        <location>
            <begin position="1131"/>
            <end position="1232"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2078684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1253" mass="142857" checksum="4C24D0A6CC003AFE" modified="2022-05-25" version="1">MNSNQIQHQQLQMMQLRDIKYTLNSWITPSVLVICSPNVSKIFQKSGNSALEEILSQYVLDKKKLSVRVGESSYDINDFKLNFVTFKHLDPQHYEQVLKDNAASVDTLATDEYHSNFQLRTKQDVPYFHKICQEPTPWFERYRHNFIKSSHNLELETFSHPIACILAVSSEDSNPIGMLVKMFDSSKPPPLFQQYNIDANIPKLYMLVHPEHSHAESDKRLAEMKQTFGSNQSYLLRVNSKEDYCGLSYDQSTAHLNPLTNIINEIISKHIPAYIEKTILELNENIATNKKSFFSKVKVSFRFGGNKKANEQEVEQANSMGSIEQALRKVADLYFILQDYEQSLSNYKTLVKEYNSDKSPNYYASVNEMIGICNLLLNKECESYFEIANTYYQKASLNHCLARVTMIHGDILKRKNNYKLLAELYKSTAERENLDAFCSAILFEQAGFSLLQISPSPLFRKACMRIVMAGDKYSEYSKQKHALRCYSFSYSIYEGRDWNLIDDHLHLSLVRYSFFQGHTSDAILFVNKLLERNCQSPEKQNSIIREFLYISKTYTKENYIGELPIPSINNESISVFLNDYSSDENQAVWQEMEEAFKKEAQIISKPQLQGFDLWKRDLFIEKEKERTAVVEEQITVSVELKNPLQIPLQFNRVHLVATFKEISDDPNSAIEESNMSPLDDEAIAKMDSTPFKVEPMDILLGPSKTKRITFTIQPLKPGLLQVKGLGLCMCGAIWGKREFKLKQKRLNKTKAQRESVMYEPNLALTFRVTSPMPRLEAQFIDFPNALYHGELKQAKLLLKNCSNKMGLKNVRVRTSHPTSICFGSNMDDASVIKSLDTSVQLNIELPPNATVLVPFWVKGVNVGRHPLRCLVYYESEAGNTDLKYRLARCQAQFDVRPSIRISSLSQQSSTDLNAYLLGVEIDNPVIGGGGESFHLKQISSISKYWKLEPLSYAGDVPNPSIVTLKPGQFTNLFFRIVPSAPITKQIQDEISISNISFLANDPMYDASSFPVFPFLEYEKLGLDLLQNGGGNSNSIFNSNNINSSSSALTNPNSTNDQGGNGNDHPNHIDLLVFWESDNHLMGVLNSPNISFLPTHYNDSLLSLPWGSWKNTTSPTGKNHMIYPSLKLPLRYQIISEKVVKHSSFETQPLCTVPVTLQLSNCSFTSVLELSIETLLPHENIDQTSRHTSQYFWTGVTKYTIKLAPQQIVDLQLHACFSQYGVYNINRYKINVTLSNDTHKDIFSTTQHLINIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q5K8</accession>
    <name>A0A8J4Q5K8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sjogrens syndrome scleroderma autoantigen 1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004341</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000151">
        <property type="protein sequence ID" value="KAF2074366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q5K8"/>
    <dbReference type="OrthoDB" id="28939at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009563">
        <property type="entry name" value="SSSCA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051888">
        <property type="entry name" value="UPF0148_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16537:SF1">
        <property type="entry name" value="PROTEIN ZNRD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16537">
        <property type="entry name" value="SJOEGREN SYNDROME/SCLERODERMA AUTOANTIGEN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06677">
        <property type="entry name" value="Auto_anti-p27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="224"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="368" mass="41421" checksum="BACA4053BDAA58AC" modified="2022-05-25" version="1">MADASMLLGKKLLQGWAMLDSVCQLCSVVPLMEDRKNKVITCVTCNQFYTRDNNNKIIILPSDQQNSIKINDNEVNISVSEPPVSTTSSSTSTSSPSSSLNDKHQQKLKEDEYMLDKDYYFYSDEDEDEEYVPLTEEEKQFLDKKMKKSEEFSSKMGQHLLKGWALLGDVCPNKECFGVPLMKDREKNFVCVSCESSGLTIDDLVKPTTKQQTPLPTVEKLQINSNSNSNSSSHNGDINTNNNNNTKYLTPSKNQITDSTLTSFSPPYPEPDHKRLRKEISPSVNNNFSQTKPTTIFTQGSSSVLSEEISDLPDVTNSTIKVLLERMKQTNITLESCQDQNEIPGLCALIREYSETMSSLLSLKKNWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q6Y3</accession>
    <name>A0A8J4Q6Y3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Arylamine N-acetyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003332</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arylamine N-acetyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000103">
        <property type="protein sequence ID" value="KAF2075356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q6Y3"/>
    <dbReference type="OrthoDB" id="17550at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2140.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001447">
        <property type="entry name" value="Arylamine_N-AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053710">
        <property type="entry name" value="Arylamine_NAT_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786:SF0">
        <property type="entry name" value="ARYLAMINE N-ACETYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786">
        <property type="entry name" value="N-HYDROXYARYLAMINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00797">
        <property type="entry name" value="Acetyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="282" mass="31844" checksum="49BD8DCD135E8C9C" modified="2022-05-25" version="1">MDFLTLFYNHVNLPIDFKVNNLEDLEVVMKALCVSTPFNNLDILDGSFVELNRESTIQKVLIEKSGGQCYHISVMMYLYMIEIGLDATMFKVLSSDKNPTPMSENHISCLLKYTDGNTYLVDVSIGVFCPLKPILIDSETIITGVNGNLCRSRSNGDGTYLFECKPNVEQAPWFCMYHYNLEPVGFPEVRKCQLNMVNDNVVFLLNSLPVIFRFTQDENKAINGTVTLSGNSLTENRGAKEKKPITDSAQFKEYLVSILKCNYNFEIKPQYARDGIAEAAFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q7W0</accession>
    <name>A0A8J4Q7W0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LYR motif-containing protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002482</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I LYR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000068">
        <property type="protein sequence ID" value="KAF2076241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q7W0"/>
    <dbReference type="OrthoDB" id="10258445at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090324">
        <property type="term" value="P:negative regulation of oxidative phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20265">
        <property type="entry name" value="Complex1_LYR_ETFRF1_LYRM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045296">
        <property type="entry name" value="Complex1_LYR_ETFRF1_LYRM5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052000">
        <property type="entry name" value="ETFRF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21024:SF0">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN REGULATORY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21024">
        <property type="entry name" value="GROWTH HORMONE-INDUCIBLE SOLUBLE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13233">
        <property type="entry name" value="Complex1_LYR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="158" mass="18250" checksum="F216858882A125E4" modified="2022-05-25" version="1">MFKTYKFINVVSTTRLMVSNRSINSSGINISGSSSGSHQFSSSNNNTEKDHQNSDNKELRYAVKNLYKHLMYLGRLSFLGVDYIRDKAKPQFMANSHLTDPKEINICLERTKYVIKEIEALNRLHKYRSLKKSYDHEFQKINDDFLSANIGDSNSNSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q9P3</accession>
    <name>A0A8J4Q9P3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">AAA ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_001200</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family. BCS1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000027">
        <property type="protein sequence ID" value="KAF2077501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q9P3"/>
    <dbReference type="OrthoDB" id="10251412at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057495">
        <property type="entry name" value="AAA_lid_BCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014851">
        <property type="entry name" value="BCS1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050747">
        <property type="entry name" value="Mitochondrial_chaperone_BCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23070">
        <property type="entry name" value="BCS1 AAA-TYPE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25426">
        <property type="entry name" value="AAA_lid_BCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08740">
        <property type="entry name" value="BCS1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01024">
        <property type="entry name" value="BCS1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="BCS1 N-terminal" evidence="14">
        <location>
            <begin position="63"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="262"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="469"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="477"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="495"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2077501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="537" mass="61005" checksum="EE0D3C88188B4CF2" modified="2022-05-25" version="1">MNPEPTPNVGFGGVDTSTWMTSLLSIFLTVPTTTSTTDQNGNNTAAVVGGPRYEYMMAALSILFLTWVVTFVKQKLIELVDSIRSRFYYTARLSYPDSMYMNFLEWLGSQDVLYGTKGVSISTRYFEGGTEDESLNLKYLPTGVQWIRFKGYFVSVSRTSSEKSGLDGLKEDYLDLTVFGGTKKLITEMMNVAIEYSTTMTKDKTKIYSLDSSCTYWECICIQNKRVLDSVFLEENVRSTILNDLDKFVNGKEWYLNTGVPYRRGYLLYGPPGSGKTSFILSMAGKFGKSISIMNMAKGVHDGNINGIIQKCPKNTILVLEDIDAAFVKRSSNQESLTFSSLLNAIDGLASSDGRILMMTTNYIERLSPALIRPGRIDLKVHLGFATPHQVELMYQRFFSEEYHHQVKDIIDRIKDDKISTAQLQGWFIIHRDDPDHLLETVDDFLESCRLELKNNDIITASMKDKEVKLNGKKKKNDSDLEFEHDGANIKKEGEEEEEEQEEEEREKDLEIEKESDTIYLNGNSSIIKRRVKINDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQ75</accession>
    <name>A0A8J4UQ75_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide-like helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008374</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000510">
        <property type="protein sequence ID" value="KAF2070306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQ75"/>
    <dbReference type="OrthoDB" id="312680at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="247"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="322"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="606" mass="70301" checksum="BFF301385F2C8BF7" modified="2022-05-25" version="1">MNKSITPLYRLFNSIKQKTSSNITLPMNGNHSVSTKYINGNRDANLKNHRIYINKYIFNRNNNNNSSNNIFNNSFNNSNNNRTYITTTKVLFNQNNVITQVQDNDIDVQYKSLIDNALEEFKTNNIQSSLNSLNSAIELCANKADAYILRGDINLEILKNLEHDSFSDYCKALEFALPTSHIQILFSIAKSSLLKTTNDNQSYLSYLEKVLFIDPNNELSRYILNGFYLRSGDYQQALDNIEKLNGVYKFLSLGDLALGQKNYEMAIEHYKKALQTYNMEKEQKESSSVGGTVYHNKFVLFNPEGHQRIIDKDQSTYLSPLYHVYNGLGVAYQSIDKLKESYECFKKLSELEPTTKFGYKMQSFLQFTMGNFEQALQDIKKAIHLDTFYPTNETESNVLSKIDLNLQLGMYGEAAFELDRMIQISLHFHPWDSEAIEKRAIYFTKLVKIIALVLNHAPITDPAIGSRKEIVQVMKENFPDSKFNETDLFRETPDEKIHNFLPYLSMYSKIYKIILDKHAGKMDDQTKFNLECRYIFFNYAHRLLEDSLYLAFPSQEEIPLPTGDNHQLILDIIYDQEYNERSQALVRQELHNLMIDLTKEIIKATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQ78</accession>
    <name>A0A8J4UQ78_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008358</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000508">
        <property type="protein sequence ID" value="KAF2070321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQ78"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="477" mass="54359" checksum="3A61149C303FFB31" modified="2022-05-25" version="1">MTEPFYLIYRNQYIRQLIRNLVCQDVSIRVDEEYLEENHQYLALFHNKHKIDYNIFICFKGNAGDYLDINNSNRDLINVVELSINDDDFDLSEIHDGVRKLSLTTENNDTSVTGKLPPSITWLRLTDDIHDDHQPSYTQQILSNLPSSLQKLELNIPRESITIPCIMPESLTDILTDGLLYYDVMKWFVVPSNRVYKSCEIYIDSVESFEWLLANKWIPKVRITRSGSNMMAGRYQLPSHVTNVEVYGIIEDQSFFPQTLEALSCGYGTPISHLAHLKVLRIGGDYPINLERGVLPSTLQELRLDYNHPLESLCANVLPLSLTELYLGVFNQPLNAFVLPSKLKTLYMQNFRQPILPPNSLPVSLINLSIGGFNGSFDQYQPLDNLKKQQVNSLNPSLATLLTNVKKLYLLVFNKGGNGDPSGCLYNTSIRSLYLEFICEKALYPNTFPLTLKYLTLVNANLESDDIIPSGCTLLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UV44</accession>
    <name>A0A8J4UV44_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TPR repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_002472</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000068">
        <property type="protein sequence ID" value="KAF2076231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="77564at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033053">
        <property type="entry name" value="Hir3/CABIN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15502">
        <property type="entry name" value="CALCINEURIN-BINDING PROTEIN CABIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15502:SF7">
        <property type="entry name" value="CALCINEURIN-BINDING PROTEIN CABIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="175"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="106"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="395"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="506"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="658"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1493"/>
            <end position="1525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1678"/>
            <end position="1698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1794"/>
            <end position="1861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="109"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="395"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="423"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="433"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="518"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="660"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="690"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1493"/>
            <end position="1503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1509"/>
            <end position="1522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1678"/>
            <end position="1695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1794"/>
            <end position="1817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1826"/>
            <end position="1841"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2076231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1873" mass="214686" checksum="CC142879B50EED17" modified="2022-05-25" version="1">MIRFVTIDTVVDQQEEEEDDDQSDDSSSNTTGDESETEPDTDTDLEGRGSRGHNRGNAATGANSIEAQEAYNIKLYERALRLQQNNDILQSQQLFQELLNTPLLKNIRNNTSGGSSSSNSNTSSNKKEHNITSSQMGMLKYSSLRNMATIYEGLNDKQRSLKLLLEALDLDGRDVVVWYNVGRLAMDIEMWNIARRSFEKAIEASPTHWLSLERLLELLYVIGDDQSCFNLSKSMIDTQFNHIKANIVLALLYYDSETTKDRAMSLLTKYNTSESTKSYILSLRSKRESLNTINSSITGEKRIIIDSEFIDLDSKLTYDSFSSLLIDTYNNRISNSNTPTLTTGSITTLPSIYSPIQFNIPNDILDKMDLTNDNNNSGTSNSSNNNDTIVVVDTPTTTTTTTEPTTTTTTTNTTNTTSTEPTPTNPTTPTPTTPITSLNNSTGGITPPSSKLAGQITITSRRMEARLKAGKKEDEKLSLELEDYFERLLNTNRPVSQEVLTTLYQSVPESENKEKKPNSNQQHNGGNSNNSSSSSSKSNNLLLEKQSLVQFIQRLNDQKQDGVSVLEWMSGTLDQITLICSDFQLQGIYTTLLNKLFTMKNIIQSHSLSYSCQSYYLVFSELMFDKYLAKKEKQDQLNKTKQKQQLIARQQQQQQQQQKILEEQQQKDKDKDVVNLDQDVNTTTPGPAATNDPNDSNSNNNSIDSTTVMEDKIVNDEHYKPNEELSNLAFYIQQLCLNFNNRNDNSEKYSITKDIKYQIRFQWLLARYSNDLGDSSLSLYYFKESEDLFNQINNNENNYDYDDDTIVIDQDKLIKDLKLPFVSLYHCKYDKIISPIILSEKISQIQDQEEKNSAGQLFNSKNYEGVISLLEPLFSDSLCFIEFNNSNSNGASNPNNNNNLIIELKSRDTMDNLLKSYLNRCDLLLQSYLNLKDIRNVLKMLVILLKEMTTFSNLTDKINQYCNLIGNLLKHPFMKPQPIKDETCSTLLSISTRLMFKFYKKSPSFQLLTLVIDLYSLDTWRPALTEYHKITLYMMLHNDQSKKRQCDTAFLQEYIILLFRFLINDTQEIESVAQEFGGFFDQMKPEALALRVMTELCQCIHCVYSIKLINTIEDHHCKSTSKATLSPSVYMQIYVIMKILSGDEDDINLPLNQLINNDTTIPFLQQHANRKEFKELTEKIYFKFMHPPVRVIKYKSMIDDYLSGKVDEIVDLPDFSSPPRINFETLFSKNDDHDNIDIDDPLFDDIYTDIYYMYAMHQEKSIDELDLFRSLLIKDLYCNPNRIATWQSLAKSYQDEWTREVNLNGVLVNDNNPEIEDKIESLFINIQIIYKHLSHLTSKVEDYLQLSMFSYERQYFIPLNQIGRGEKIQKLCKESMYYLDKCLEIDPNHKDKWYFVYLKGKIQKKQQLSPLVYMDTLFNSIQLLPPLSKKNPPINPIYRLHSDRLKLLLPLDKPIDEQLLIDMEKFNFIPQKENTLSILLPFTLSKPSLSPKISNENVKTQTSVEDDLSSSSSSTTSSSSTSNNASKRDLMIENSLLALFYCKSLVTYHHQSIHRISWTIRYYTKKEDYSINALSQFIKLMRPKLSRLSKTCVWSLWNDGYLADGKLDRYFQKYYRFFIQLLEENNDMKNLEVIYFKIKTDPKLSNESSQCYLSCCKVASYLITQKKLNINNSISSNNNSNGNSNSNSNNNINLNHSGDQMIIDSSENESLNSTSNTQVLDIDTIFKYVWELYCDVTNNEQHRNSVLSLMSNCYQLANNGNNSNTPPKTDELITFFEKKFLRSDKRKKKIDDITSTSTTTTAQSSLSSPTSTSPQTSKKQKTDNSTTTTTTTTTTTTTTITSPSDHHAPTLDANANNNNSNQILNKFSTALPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVJ4</accession>
    <name>A0A8J4UVJ4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF155 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_002065</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RMD1/sif2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000054">
        <property type="protein sequence ID" value="KAF2076636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVJ4"/>
    <dbReference type="OrthoDB" id="21494at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003734">
        <property type="entry name" value="DUF155"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051624">
        <property type="entry name" value="RMD1/Sad1-interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255:SF5">
        <property type="entry name" value="DUF155 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02582">
        <property type="entry name" value="DUF155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="402"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="DUF155" evidence="4">
        <location>
            <begin position="199"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="137"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2076636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="425" mass="48942" checksum="D6C849B388639BF1" modified="2022-05-25" version="1">MQQLINLIKVSPFLDKKYKKNIQTNGNNNDIDLDQFTNLEDMEKIKLPPYKINDFQVKRNKYPGHQPCLINGLYSSTDHSPLSQSGVQNLPKKVTSFCISDSINMEQSYSVIDGLNFKPKAYKYFYYFVIPRESNPNNLSNPSSPNHSTQNLNPSHQHEFEKIPTQNSINSLHQLQHDSNSTSSLDELIDNSNSNLQLVVVYEYGVVVCWNLTPTLQIKILSLMEPHIISPLSPRLVDEMIIEKNNTTNRSTVIINDHILFPTGSDNLNSRLVLSNMISQSLKISLFEETLFRMIQLTHHVPSQLVHKGKVDMPGFQVGHVIGNLFKLKNSLSIIIQVFKAINRWSTEYDEPTKNNIQTMSRKYLDIKYRLKSIDQKMKYVDDIVSVCKSEHETRGSNRLEMIVIILIFFELLFMVLQFIYSIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWD7</accession>
    <name>A0A8J4UWD7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001219</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000028">
        <property type="protein sequence ID" value="KAF2077446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWD7"/>
    <dbReference type="OrthoDB" id="337581at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097196">
        <property type="term" value="C:Shu complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28498">
        <property type="entry name" value="ZINC FINGER SWIM DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28498:SF1">
        <property type="entry name" value="ZINC FINGER SWIM DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="3">
        <location>
            <begin position="95"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="173" mass="19497" checksum="7E4BB1583F27FB14" modified="2022-05-25" version="1">MNHVVNNDAQGDKAKTEVSRTPPPPIQLVEIIKHIFKNNKSNTIGEDTLLSLYSIFGKPLFASLDILDNNLISVYIFLPSKRYFYVIEGRKGAKYMCLLKGDYCSCPSFNYSVLLKSDSLYCKHQVSAFLSEILSNAPSLEFDDSEYITKVLEIEACSFKTPKSTKKPVNKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWL6</accession>
    <name>A0A8J4UWL6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001043</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000023">
        <property type="protein sequence ID" value="KAF2077656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWL6"/>
    <dbReference type="OrthoDB" id="24136at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052014">
        <property type="entry name" value="Dictyostelium_Tiger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31341">
        <property type="entry name" value="IPT/TIG DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="9"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="324" mass="36272" checksum="5D88B98571504B89" modified="2022-05-25" version="1">MKGFLLNQENGHYYAYSNTKANYQDSVNVCQTYTPPDGFTGYLVTVTSKDEHDWIGSHLGAISTIWIAGSDLGDRGNWTYNVGPEKGQILYDLYSERTYTFTNFPYTEPNLEVGENYLISIPNSKDGWNNVVASTQSNYICEFSPIDEPFIPTVHSEGDMVTVNVNGYDISTLEIKFTSASQPNFNCQNIKKVGENTITRQVPSGTGKYSYTLSDKVKSSKSVPWQYHPPHISMIGLSLETVTLVGDNFGNDVSKIQITFDVTGTNTECNNINIIIPFKQMTCSLKLLPKILPFQVVVNSISSRYNKTSLYHFDSKSIFLIPIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXJ2</accession>
    <name>A0A8J4UXJ2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="17">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">CYY_008121</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 17">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 17">Membrane</location>
            <topology evidence="1 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="17"/>
    <dbReference type="EMBL" id="AJWJ01000475">
        <property type="protein sequence ID" value="KAF2070558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000021">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000126">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000014">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF212">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 16 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 15 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 17" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="220"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="443"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="487"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1018"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1051"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1101"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1138"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1170"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1212"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="20">
        <location>
            <begin position="180"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="19">
        <location>
            <begin position="278"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="21">
        <location>
            <begin position="987"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="23"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="101"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1320"/>
            <end position="1342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="111"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="156"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="14">
        <location>
            <position position="555"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="555"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="555"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="556"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="557"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="557"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="663"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="705"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="728"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="762"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="842"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="843"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="844"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="935"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="941"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="961"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="964"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="965"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="965"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAF2070558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1342" mass="151614" checksum="7522131E57E32589" modified="2022-05-25" version="1">MNNNNNVRRSGQDIDIDIESEALTNNNNNNNNLDNSNNLSTSTNTDASSSLNTGWILDSISGRKSPASMDEKASLANQDVNQYMMEQQQQQQQSNISGIVVPNIDSDYNGSTPSTNSSPPKSPTLSRASSSHMNGSSSQQPGQRKGTVKSSSLRSNKPKKKAKPLKKLKKKKYIGTQRDIYINQRERNALYKFCNNKISTTKYTPFTFIPKNLYEQFRRAANFYFLVIAVIQLIPGISPVNAYTTWIPLIFVLLVTAVKEGIEDFKRNQSDNTVNNLNAKVLSNNQFSDLPWKQLQVGDIVKVVKGERFPADLIILNSSEQHGVCYIETSNLDGETNLKQRQAIPQTFEILRDEQDLSRFNGTIECEHPNNVIYVFNGTVRMTEEGTRYPLNNNQTLLRGCVLRNTEWIYGVVVYTGEDTKLMQNSTDAPSKRSTLEKLVNRALINLFAIMFAVCLIGTVVSVVYTAKYKTDAWYLGFKDNAVRKAVLNFFSFMIAFAVMIPISLYVSLELVKVAQAIFIYWDINMYHEESDTPAKTRTSNLSEELGQIEYVFSDKTGTLTRNQMDFLKCSVGKMVYGNLEKEDQPSTSSKYGVQMDGIPGADPKFGFKDRRILDHLDEDPNSEQAFLINEFLTLLAVCHSVVPDRPNKDDSEIIYEASSPDEAALVYAAKNLGYAFYNRDPTGVFVNVRGKGIERFELLNVLEFNSDRKRMSVIVRSPQGRIILYCKGADTTVLPLLRKDQEDIYSVTLEFLQDFAADGLRTLCLAYTFLEEEEYAKWNEIYKEAAIAIQDRDAKVDKAAELIEKNLTLLGSTAIEDKLQVGVPQAIANIIKANIKIWVLTGDKQETAINIGFSCHLLNSDMRIIILNGKTEEEVQNQIQGAIDAYFSDDIEDHPSSGFALVVEGVCLNFALEGPLKTVFLQLASNCKSVICCRTTPLQKAQVVKMVRDTLRAVTLAIGDGANDVSMIQAAHIGVGISGNEGMQAVMASDYAIAQFRFLYRLLVVHGRWDYKRNSRLMLYCFYKNMVFAMTQFWFGIFNMYSAQTIFDSWSISIFNVVFTGLPIIVYAILDQDVSAESSMKYPQLYASGQKDSEFNLRILWIWILEAWVHSVVVFFGVYGIYQYGGNLLESGLTLDLWSMGQNIFIVVVLTVNLKLALETRYWTWLTHFSIWGSILIWFIWVSVLAVIPGIGSSSSGEVYQVAFKVFSSAYFWFSLAVLPVICLVPDTLYKYIQRDIKPYSFQIVQELERIYGKPSDIMDKDLLQQQDDGRKYKSSSNVADIEELNVESSSNSLQNEKRKKKRIQFLSWMKSSKVHKKNTGFAFSHPNQNKTMKDPSFASD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXT7</accession>
    <name>A0A8J4UXT7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_007213</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000372">
        <property type="protein sequence ID" value="KAF2071460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXT7"/>
    <dbReference type="OrthoDB" id="20134at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050940">
        <property type="entry name" value="Actin_reg-Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46485:SF5">
        <property type="entry name" value="CENTER DIVIDER, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46485">
        <property type="entry name" value="LIM DOMAIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="23"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="297"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="655"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="733"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="321"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="349"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="377"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="405"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="655"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="692"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="739"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="798"/>
            <end position="807"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2071460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="807" mass="91513" checksum="1294B091C941C406" modified="2022-05-25" version="1">MSLFDSNKSKRLSKIIDYSEIKFDDLNQVGSGGFGKVYAGYLNGRKVGIKKITIYDNDPNKEILSKFLLREIYTLKMLSHPNVIQFYGMAVKDKSYYLLTELVSGGDLHWYIKHKKNKMNYKLILLIARDIASAMKYLHDNGVIHRDLKSTNLLVSEGWVIKVCDMGLARTVNSVEKSKMTICGTDDYMAPEVMIGEEYDQSCDVFSFGMVLVEMILRESLTPRMRNEDLGLDKPYLLNKLPKDCPDEFRQLILHCCKIQPSARPSFEQIHGLICHLLEQMTSLAEPEYPSLRTFKPPDLHIPVSKNKPSQFSFPRPYQESSNNLANTLNASGNSNQESKFSFPRPYQGSTNNLGNSLNNSSNNIQESKFSFPRPYQGSTNNLGNSLNNSQQQESKFSFPRPYQGSTNNLGNSLNSSSNNSQQESKFSFPIPYEPSEESKIYNNNNNNNPLLSNQRSYQQPFLSCSTDSDINDLQCMAKDINFSDSPSGLCFSKPFQSIDDQFNINNDDPLQIDDLQYNIVQDNNNSNDNDDEQNLQVISFEEFELLDFPRPYQPDGVENTQPTQLIETPDYSFPRPWNPDIDPPIKSRVVTSIARVNTPDQLKKQESKVIQSISINNEKVDFKEENNNNDKIVNTSKPKPVVIPVTKTPDIIITTSDNNTVNNNNNNNNNDRTNSLSPNETSPLPSPTSPISPTSPTSPTSPKSSHGRSRSLDFKQSTKAIIKKFEELTRFHSQNNVKKGETKETSTTSTKERSRDGKDNNDGKEKEKERDKDKDKKKEKTPPPSPTKVPFWKSKPQPKDSIKTCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UY34</accession>
    <name>A0A8J4UY34_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_006698</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000320">
        <property type="protein sequence ID" value="KAF2071985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UY34"/>
    <dbReference type="OrthoDB" id="207120at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF116">
        <property type="entry name" value="CAP, ISOFORM AC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="162"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="52"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="73"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="146"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2071985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="221" mass="21919" checksum="FED21D73B6D31CBE" modified="2022-05-25" version="1">MSFKPAPLKPAGAPLKPAVGGAPPPVGARPGVAPPTGAAPLKPAGFGAPLKPAGAPVGGSAPPATAPRPAGVAPPTGAPLKPAGAPLKPAGFGAAPLKPAGAPIGGSSAPPAAAPRPTGAAPPTTAARPAPVAAAPRPAPKPVVKDDDDDGWSDDDEIEAPKILCVCRVIRDYIPTNNDTLTLILDDLVYVMKKPSKDMWEGETKGVYGTFPSKHVEEVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYA2</accession>
    <name>A0A8J4UYA2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BEACH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_006280</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000283">
        <property type="protein sequence ID" value="KAF2072405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="26681at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06071">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01201">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1540.10:FF:000001">
        <property type="entry name" value="neurobeachin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1540.10">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036372">
        <property type="entry name" value="BEACH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050865">
        <property type="entry name" value="BEACH_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046851">
        <property type="entry name" value="NBCH_WD40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031570">
        <property type="entry name" value="NBEA/BDCP_DUF4704"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046852">
        <property type="entry name" value="Neurobeachin_a-sol"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023362">
        <property type="entry name" value="PH-BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743:SF160">
        <property type="entry name" value="BEACH DOMAIN-CONTAINING PROTEIN LVSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743">
        <property type="entry name" value="BEIGE/BEACH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02138">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15787">
        <property type="entry name" value="DUF4704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16057">
        <property type="entry name" value="DUF4800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13385">
        <property type="entry name" value="Laminin_G_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20426">
        <property type="entry name" value="NBCH_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20425">
        <property type="entry name" value="Neurobeachin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14844">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01026">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81837">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51783">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BEACH-type PH" evidence="6">
        <location>
            <begin position="2665"/>
            <end position="2765"/>
        </location>
    </feature>
    <feature type="domain" description="BEACH" evidence="5">
        <location>
            <begin position="2776"/>
            <end position="3066"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="3205"/>
            <end position="3246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="284"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="650"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="748"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="2179"/>
            <end position="2236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="3050"/>
            <end position="3075"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="3427"/>
            <end position="3459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="284"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="348"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="652"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="713"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="2179"/>
            <end position="2233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2072405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="3459" mass="391938" checksum="D9CE5D5757D7AF46" modified="2022-05-25" version="1">MSSNNSNNNNKKEVSDFLKPVQHHINLNISNSNISIGSSSNVINNNSNTAPASIQRPAHSLKTIGSARFKSHPDLHVIENDVWRTKRGQEISDALWASFKSNLNSPETCNEILKEFVLHYNSQYKNWNKDLHPEHYDTKITAIKSKAVPMLDLSTTQFITRQLCSFVLSLSKALTDNNLDIPDGLHYVCLINILSRSVPNTIIILQENALSHPFQAARLAAFRLSGFKDQKDAAFVKWWPYLKVLLANCCQIATNFIPDKWQWHQITKLSSSNNIDTADKDLTSCNSNSSSTGTTGTTTPPSSSTSPNTFSPSTSPSLTGHGTWSVSSSNNSNIGNLMHASNMSPLRSSSSGQSNNSLNSGGNSHDTRANNSNITNQIFKFGIIGILIDCLQFYIDFYSLKQPKDSSSTFQYLLIDTLGILIYASQGSYKCKENGNEMKTIVQSFGKKAFTIPEKKQTSLQLEFKFQLLVLRVVKEFICNNVSNARLFYHFNGFERIQSTILWVSQCFKPSDNHVLSNELSEEFDVSLPAYEQSAILAGNIQGTSNTMYDNLNSVLSSLKYTTYSNLKSANSKSPPAFLKRSKVLQLNQLFGVLSSLSFTSISLSKVNMSSQGSGSFLNLSSSGTNINTANSASLPSNFGATSKSFMGQPLSNSLAPVSNSNSNSNTTNPSSDTNNTNNGGDDIHHSHNSSGNLSNILAASHGMMSGNSNSHSIDHLDNSNEITDSHDDKKFVNNLLIELLLEKFYSNSSSASGNSKSINSSPVSSMHNIGSTSSPNSPTSVNGSSSTTTTTTTYNFSSIHNIGHSNNVAILEHFKTQYPELQLTMLEYIVKLFTEHNQCLAVFRKHLIWDLIFSDHFYYSAVNIYSTKQDQIDELDNSNSASTTEQQQQMNDKKRTFYLFNALRLSCINVLQYFATHKNIDNFDEIYAILNEMQRSLTKPAITIELCQLLINITKSNPQGTIPSLIKLKIFKQLSQMIQFLLKNDLESTTPSSSTTSLSSSTTTTLATTPINLVKKARNILYSFLGQLIAHEELSQYAVNDIDLIETLFILLKRPETKTYSFSQIFFLMKLNPTNESTLSDIYSTYISQLTKIREETINDKLENGFELILTLLDGIKQIAKNNQKKQLLFKKFGVFIKIVTLVNADDTKERLSTLCHSVLKTIVLLMTNNPKIKKHFRNHIGYDTLRDIIIKCEGTVSKNTMDILFDMIVDSEFHTDYNYIIQNSDAVILLFNLLKYFSTDHQHEILKKFTEIVQKYTTNQSVCCNYHLIYILLDSIYKDQPMDITWGILNLIQQLGYHSVTVRELKKLFGLLKSELGDFRPPTTVLLLSTLQNISVSRNPGPQVYFDFDGKDSAIILPTFDKWPFPKGFSFCTWVRIESFFDPTGAPEYNPRLLSFLSDTGCGIEVLFAYQQIQILTVSNSIGKPIFTTPFSFEEKKWYFVCIVYSTNLISSNEIRIYVDGQQRAKAPIKLNIQGHLMNNFRIGNNSKVSGERQNPLYGQMGALNIFDESLSPSQIQAIYTLGPNFNTSFQDVEGSTKSSHTFDSSLTSHLFLNYNCRAIDGDLCLDNTPDIGGDRNYDAVLVSINPCVSRDIKDIIYCLGGIKVLFPLIPQINQSIDPNSLSMIPDEDNFKTVTNNSKLTFQVLALFKDMLRGSEAIQEEMLRCQGFSVMGYLLQQITPENLTLGSLNIFKDMSTQISDPSLVEEVYTHLLLNFSLWIKTKYEVQKSLIVMIKQIIFEKYDQVCDIITVQKIIDIMCDFYWYEHSVIGSINNNGSNSTTTSKSIFMSHPFWSKEILSKRPSPCDIKDLRQVLYEVLRLLLRTPSTNDVTSIIRFLVFCLDSQQLVETLEFILDLLSSNNTNGGFQLFFEELLTLGGTDIFLGLLRNVDEKVRVAALQLISKIHCICSAPNTNYKRKPKIFQDSIIICRSLQLFPFTDLTYRYLLAFTLGFNFTPQTFNSDPNMDELTAEGYVEIKNNEILAVILKLLLGAESIVLRQQILQEIKMLVTICASNRTSCLQIEKWPDHLFSIINDTEVKNDTQYSNVIDLIIDIMKILAIHSFHDSKPGFKTLEQIIATLRPYSDRGVLDYTTIIRTLLTNIVCSIKSEAHTLVSDHPSTLIKKPKKILDNIVSYLMLAEEFIFYTPKEDLSISFTLLELLNEDTEANNSLANSINIGNSSSNVQSTSPGSGGNHSAHSSYSNYSSTNFSTISANHGGNSNNNNNNNNNSKLHLNDRNEWEDSGLVKEILELVDTLKLYSYSHDIFSTSNMQVVGKTTNLMFHTHHQRVILRLTLEALNESCNSKVDSVNVVMSHMIRLKHLLEKDFHSKSEDSNARCLFTLSSMIKMLKVTIDENMTNHQKAMVPFMKDIIRKFKSSIIDYIVMKQIGNIPINGLGGTPNNANNNGGGSSSSISIGSPLGNSPINNQKINEIETQITTWMGELTDSLRIVDFCTIVVNSLRWLQLVEYIDNNSHTYFGAEEKLLIQFIEKRKKKASKNVQYIDDKESENFTLAERKADNDLKSVQVNLWNPECERRSVYLTSTREQLNNTTVQWRKILRSLTNERGPWGTTESLVHWKLDKTENTSRMRLKLKRNYKFDEHLNCAIADESLPNAQQEVPQQDNIVIPLKKQDTDEDSDWTIINDEDINTLIQQQQLQQQQQQQQKEKLLYTTTCEWINPMNSKKGRFDITTSHLIFTEEQSEQEIEAGIIPKFKRWPTEMIKDVSPRRYLLRGSAIEIFLKDKTNYFFNFKKTDRNKVFSKINSARRVFFRAEPSLSPADTLKKATADWQQRKICNFDYLMILNTVAGRTCNDLTQYPVFPWVIADYKSPQLDLSKPETFRDLSKPIGALNERRLQIFKERYESFDDPIIPKFFYGSHYSSAGIVLFYMIRMEPFTTHFLQLQGGKFDHADRMFHSIELAWENTLTSSTDVKELIPEFYYMPEFLLNQNNFHFGTKQNGHVIGDVQLPPWAATPQDFVRINREALESDYVSAHLHEWIDLIFGFKQRGRFAEEACNVFYYLTYEGSVNIDLIEDENTRKATESQINNFGQTPTQLFPKKPHPTREPRNESQNSILKTSPNLLQAYFLKISSKNPIVYVFIPENNPIMSYLVADRVTVIDKSRTTTNHKWFPNIPNDKISPFTFELDPSTTTKRRIGLPFANDVTISPNCFAITNDGRYVISCAHWDNSFKLSFVDSAKLIQSVVKHKDTVTCLAMASDGQTLITGSKDTTVMVWKIHNNNKSGAPRFADVPDHILYGHDDEITCVDINVELDISISGSKDGTCIIHNLKHGEYVRSIYLPRQSPVSLIAITYQGYIVIYSQADLMIYLYTINGQLLKSIDTHERLHSMIVSKDNTYPEYLITGGERGTVVIRTLYNLRPTNHKLMFGTPIHSLAMSSDQRHLMVGLEDGRLLIIAANNENENQIQNQNYTPNNNNNDNMNNINHMNNNNNNNIDHFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYN3</accession>
    <name>A0A8J4UYN3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_005457</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000218">
        <property type="protein sequence ID" value="KAF2073230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYN3"/>
    <dbReference type="OrthoDB" id="302966at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16534">
        <property type="entry name" value="RING-HC_RNF5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045103">
        <property type="entry name" value="RNF5/RNF185-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12313">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="168"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="217"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="295"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="106"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="295"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2073230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="351" mass="38864" checksum="67C390057B4A38D4" modified="2022-05-25" version="1">MSEISSSNDSGNESNNEPNTLTTDNRSTTTTTTITTSPSSSTPRVLSGTFLHNRNETRSQSSNDGDENETESIIFNSTPTPTTTTTTTTTSTSTTAQIEDQEEQEEHSSPIKENESNDDIHTNGEINDNDIDDIKENNNSNNRSNSNINNSNNNSNNNNPTESSAFECNICFDEVAEPVVTQCGHLFCWSCIFQWLQHNSSQQCPVCKAPVSQDKVIPIYGRGGNTNKTDPRKNNSSIPQRPPGRPEPNRGNNTRANDPFHDFFPNQYQGGLGGVSFSAGFSLFPGLFGVHFYNQGQQQQQQQQQQQQQQQGGSPQNRQGSPQNQMDPDAIFVSRMMFVIGLLILFFILFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V059</accession>
    <name>A0A8J4V059_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_004723</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000172">
        <property type="protein sequence ID" value="KAF2073978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V059"/>
    <dbReference type="OrthoDB" id="429183at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38483">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38483:SF1">
        <property type="entry name" value="ION TRANSPORT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="7">
        <location>
            <begin position="143"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="33"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2073978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="296" mass="33769" checksum="DA71E326060F8514" modified="2022-05-25" version="1">MNTSQNSTNNSSSSSKYTLSELKYDDLNIIPTMSSSPKLNANTSRNSPKLYNNDLNTPSSNINMTSSNNNNNNNNNAILTSSGGIDDDEDDDFPMTNEYHEDGVGGSRHHHSTAHIDYHHHSSAVKDRECSSYADRVLFSNKYYYSYIFMILINLTLILWLIINLIKKNTAIPSHWLFITLDILVNITLAVEILLQVISQKKKYFQQWSNLFDLLVLVLSIAGLFMYFLASNSTKTFDFEGIFIILLTAIRYGVQFLRLLALIKRQSMKNSTFNSRIDFTELKESDLDFDIDSSDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0I3</accession>
    <name>A0A8J4V0I3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002963</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000088">
        <property type="protein sequence ID" value="KAF2075720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0I3"/>
    <dbReference type="OrthoDB" id="25620at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="182"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="368" mass="42423" checksum="535340B3C70CBFD9" modified="2022-05-25" version="1">MEQENHNNITINNNNIIIHNDNKSLHSYYLEDNEKLQKENWLYKKLGCANELHRDLIWKLEVQENKIQEGKQLLNDKLQVIHGLNLKITELQKEKDQAVRDGKQLQKRINELETPSRRTSTLLKKSIELQNSPPAAPALAHACNCGSGGKQTESDEKKHVIPLSVLFKNPLLFDSIDVNQYGIDFWKYKPMITEWINSTKPLQFELLYRASQNENSIQAFHDKCDRQGPTITLIKTTEGDVFGGYNSKSWGYNFYGDKRCFIFTLINKHNIPPTKYMYNTDITNYPDKQCCEPDPTLSSCAPVFGFNPRGGSADICIKSTESFQSFPNNYIDTTNVGKSTLTRSKFFTVQDYEVYNVVELNMIRKNKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0M7</accession>
    <name>A0A8J4V0M7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Coilin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002716</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000078">
        <property type="protein sequence ID" value="KAF2075960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0M7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="127"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="339"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="411" mass="46843" checksum="8EB41BCF91B74D9F" modified="2022-05-25" version="1">MIIKLNIDDSIDNSSINNLNKFVIHLAEKDVLKSKTIDILYQLVSKRLNIKTDEFYVCIQDEYVVLPQEDSKLLLELNDFYIISRSYFTKKRSFGLPLKDESKNVKKIKLGSKSSDSDSRSSESESESTSSQSSSSEESEESSDSESESSESESSKSSESESESTNSIEKVTQPIKQPRARLTKKSAAQKKPNDFKNDIVNNYSYFVSCDDQVDQEMENQDEYENENENSNQENNTTLAVEKEFDYSIFPNLIDYPDVNDKIAFKMIMLDPTFNTFLTDFIEGKVSKIVGDKITFVIENGIEVFELDEGLLNDDKTLTLPFSTIKEAKIIQKSTTTIVNQNNNTQNNNNNNNNNNNNNNTNNRNTTFELVSPDKPNRSKRRPRQSAVSGVLKLLRQAKEQEEVEELSNQDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0M9</accession>
    <name>A0A8J4V0M9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_009234</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000660">
        <property type="protein sequence ID" value="KAF2069452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0M9"/>
    <dbReference type="OrthoDB" id="10253869at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050218">
        <property type="term" value="F:propionate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43347">
        <property type="entry name" value="ACYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43347:SF3">
        <property type="entry name" value="ACYL-COA SYNTHETASE SHORT-CHAIN FAMILY MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="4">
        <location>
            <begin position="9"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="2">
        <location>
            <begin position="81"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="3">
        <location>
            <begin position="524"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2069452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="648" mass="72793" checksum="A36503E713AF8AB2" modified="2022-05-25" version="1">MTISEPYNYEYDYKLSRENPIDFWDSISKKYVYWDVPYQQILTGTDTNPIWFKGGKLNACYNSIDKHLNNPLIRDKYAFIHESPSKKIVNKITYLELYKRVCQFATALKSLGIGKGDRVLIFMPTIIQTTIAMLSCARIGAIHNTLYGETTILNLSKVLDNLQPKLIITSNVGLSHNDNFISFPNKLNQAIKMITREQQQSIENIIIYNRKDLIVVFNEQIQGSLDWDELVRGATRDGEYIDYGYTPVDPNHIFHIIYTSGTTGDPKGFVRDTGYIVALNYHIRTSRSIRESDVLLAKGDFGWLSGHGLMCYGALVIGCTSVLFEGDALIPDLGALWRVAEKYQVNVIRVGTLLSQFYVKDPKGEHMKNYNLSCVRLILCGGEKIQPIVKSFIKSMTGIKVYEGYGSTESSASILGNPCGQVDLLDGSTGVPLPEYNVSILDPQGNQCEIGQPGELVIKLPLPPGFAVDIYRNHDRFLSFFSKYKGYFATGDLAVQMDNNNFNVISRLDSSISINGNIISMGLIESVLMGYHQAIRECVVIPLTLPKPPLSNQDHFFTLGFIVLNQQIDQNSLENEITQMVSNDKLCLLACFRGIVFVEKLPKNKSGKILRNSLIDSFNDKLILDIHNGNLIDVVTELKKSEHLLGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1C5</accession>
    <name>A0A8J4V1C5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003334</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000103">
        <property type="protein sequence ID" value="KAF2075358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1C5"/>
    <dbReference type="OrthoDB" id="17797at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="90"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="327" mass="36383" checksum="6F7160E95D54ECC3" modified="2022-05-25" version="1">MIEENTVVAQQCYCDPTFAHDGCNESGCLAIAIIFMVLFLIPLVVCVYRLFTLPKKSDNVWSAVSHSFIAVACVFRVIRSACLIARVESIFIMAILFLFPIGFLICSYFNTLFVWIKIIYHFNFSKLVEKIFPFLGKTMIGSQVLLMVMLIICSATYWPYWSTNLILGFFILYGALGFAIFGKMIWSEYKDLSNKDCGVFSQNTHNKIQRVTKLSAAAIIVTFCTFMVIIWSFIQQPLSMKNLLAFNFVGRAIEVSWMTSMLLVLSPSLQTGANNTASQVSKGSHLDDLSDSSIDIEEPESSGPTQVGVTASSPNKEEPACSGTAEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1N9</accession>
    <name>A0A8J4V1N9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Peptide deformylase</fullName>
            <ecNumber evidence="2 3">3.5.1.88</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003066</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Removes the formyl group from the N-terminal Met of newly synthesized proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>N-terminal N-formyl-L-methionyl-[peptide] + H2O = N-terminal L-methionyl-[peptide] + formate</text>
            <dbReference type="Rhea" id="RHEA:24420"/>
            <dbReference type="Rhea" id="RHEA-COMP:10639"/>
            <dbReference type="Rhea" id="RHEA-COMP:10640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:49298"/>
            <dbReference type="ChEBI" id="CHEBI:64731"/>
            <dbReference type="EC" id="3.5.1.88"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the polypeptide deformylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.88" evidence="2 3"/>
    <dbReference type="EMBL" id="AJWJ01000091">
        <property type="protein sequence ID" value="KAF2075643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1N9"/>
    <dbReference type="OrthoDB" id="276063at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042586">
        <property type="term" value="F:peptide deformylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00487">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.45.10">
        <property type="entry name" value="Peptide deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00163">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023635">
        <property type="entry name" value="Peptide_deformylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036821">
        <property type="entry name" value="Peptide_deformylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10458">
        <property type="entry name" value="PEPTIDE DEFORMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10458:SF22">
        <property type="entry name" value="PEPTIDE DEFORMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01327">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004749">
        <property type="entry name" value="Pep_def"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01576">
        <property type="entry name" value="PDEFORMYLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56420">
        <property type="entry name" value="Peptide deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="236" mass="26443" checksum="CFF1AA221B2BEC6F" modified="2022-05-25" version="1">MNKGFEISSKVLKLGNKLLRQRAEPWTREELAQTDKIGSLLEKMVDTMNAGNGTGLAAPQVGLSKQLFIFKLDSVDESFKCPPFPLTAFFNPVIEIVDEDVEKKQATGSASSSRMLNLQKYKRSIHSATNSLPKKPTDNNTIAIWESCLSMPNYFAKVPRARKCIVKFLDVTGKERAIEADGIIAACLQHENDHLLGRLLIDRLKDPNLQLVCQDEMTDNDFNDIFTHSGDFQIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1Q2</accession>
    <name>A0A8J4V1Q2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000089">
        <property type="protein sequence ID" value="KAF2075688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1Q2"/>
    <dbReference type="OrthoDB" id="443915at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="408"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="558"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="714"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="316"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="566"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="607"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="736"/>
            <end position="747"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="857" mass="97035" checksum="1D4EF69860925625" modified="2022-05-25" version="1">MNFSINQTTCSIISTSSSLTTTATVNNMKDVQYKYSTTSWRDLRYFQVLNLHTDLSFHKLMDSLYSVQQQQQSSTKSNDQELELNEKISNIIFQACSFENDFKKEELKEAYINLTDPEKRKFYWINGSPSEILNNNSSAHNNNNNSNNSSLNLAIYSLKNSNNNSGSNDITNNNNNNNNNNNNNNNNNNNNLTKSIFSPYLNNNTGKSPEQCSKPTIEEIIHVKNSKTIQLLLNWKCNSNVQEYTLLMKSKATDSYSIIYRGHNSFYTTPDLEMGVYNFKVKATNRYGIGEFSKELEINDSFFIKFLKTIQVDNNNDSNNENNNINNNNNSTILNSDNSMSNSNTTNNNQNNKKKKNNNQNSIKKLLQEISSYLNQIGKISTHCNVEKCEEIQSQLLYFQSRYQKFQLKDQDDSGSSSGSDQENDNNNSNNNDQIEIVKRLDTSLDNVNQLLASYRLIKEWRTLIKTSITEFTLSNNESQEKIKQVAQSLSKDDLSDQVKTMIYQIIIQFAKKKLTDSSTIIKSRVSTLLGLFSERKDFFGSNWCLEMDSLSKQLLNDPKKKKKVNTSSSTATTSAVATVNTSPPPPKTKLQKNQITIQSKPPKLKINTTPPIDINNNNDIIKKQVVQQQSSPPKQQLKQFVQPQQTQPQEVREKIELELNGSLSSSFGSSYSSSPSSNFNYSSFYPSSPNNSINITSSPSSRAPSTSPIEAHLIVTPPSPKQNNNNSNGFTYDNSSSISSGSGSSGNYSQMESNDCFNYQFQGFLTPLLSNLNQNVNFTSSTSSNMYISQTSHLNNTNSDNYSHHHQQQTFFQDESILQFDFFNNYNPNHYFNSNAQSQPLEIPTFSYGSNSSYPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1S7</accession>
    <name>A0A8J4V1S7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ras guanine nucleotide exchange factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002945</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000087">
        <property type="protein sequence ID" value="KAF2075763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1S7"/>
    <dbReference type="OrthoDB" id="546434at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF362">
        <property type="entry name" value="RAS GUANINE NUCLEOTIDE EXCHANGE FACTOR E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="6">
        <location>
            <begin position="371"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="5">
        <location>
            <begin position="530"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="142"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="501"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="764"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="142"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="182"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="225"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="248"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="278"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="781"/>
            <end position="790"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="825" mass="93561" checksum="BBCB94310D14BCFC" modified="2022-05-25" version="1">MMGQDQNSYQTRINDLEDENQKLRNRVGELETLNSYLKSQLEHFQTKFLESNLMNENSKDISSCLSEIYNSLSLESNNKVNTFEDDSVIDDSVEIYNEDEDYIVDESQSILNENYYNHDDEQNQYINSDSNQSSPIFYSANSSIIHNNNNNNQVQSNKPPPIPAKPAFLRRDSENSVGDGPSPSNSPRTIVPSQPISTTTTTTTQSEEPKKSSWSSAFIPKQRSDSILTQSKLNLNDSQSYSNRQINTSSSSTTSSSLTVEKKPPQPLPQIPDRRSKTSPFPTNSVSPLATSGHMTRTVSASAANAPKPHGLSSSSSSINVSNTSTNRIINAPPMNLSGSTPELPNAADLLEDEPENLDDPQLILVKNENGNYLVKGGTLEKLVQRLYYDRSHDTDFASAFLLTYRSFTTPIGLLDMLIAAFLNIGEIDETSSALAKKKRITRLRIANVFKNWIDKHYHDFQQDPELVEKMDIFIEKHVEHSLGDFGKIFKKLLHNERATPNPTWANEAPTPIPPKNKNTVNDLKFKDFDPTEVARQLTIIESDLYRKIESKECLNQAWNKEDKLEKSPNIVAFIKRFNQVSSWVATEIVRQEKLKDRVSYVKQFILIAQKCRELGNFNATMEILSGLQNSSVFRLRKTWEKIEAKPYIKKIYDDLLALMSSSGNYKEYIAALHSVHPPCIPYLGVYLTHLTFIEDGMKNTLNNQDDLINFEKRRKISVVIREIKQYQQQPYHLNVEETTINYLRNLPSVLTDKAMDKLSLMCEPREQRTSESSVTSPMSPEKENSRKDNNLGSSTSGLLKKEFSMQSLLSSFSSISKNNINNYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V254</accession>
    <name>A0A8J4V254_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_007517</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000406">
        <property type="protein sequence ID" value="KAF2071162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V254"/>
    <dbReference type="OrthoDB" id="17780at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051158">
        <property type="entry name" value="Metallophosphoesterase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31302:SF21">
        <property type="entry name" value="CALCINEURIN-LIKE PHOSPHOESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31302">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN WITH METALLOPHOSPHOESTERASE DOMAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="256"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="3">
        <location>
            <begin position="362"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="71"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2071162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="596" mass="66964" checksum="D2CAD982A27776FD" modified="2022-05-25" version="1">MIKNRKEKKTNNSNISNSNNMSLMKEEPWNPEIGEMTPEMKPAKPVVLNSPIYNAKKDYNKPLIHQTYESFPNNNNSSFSSTSGLNSNNYSNDNSSNNNKGYQFVQPTSTNIESEIEKSLTHPVKRGALIVMILVAILLPTGAYLIGANVRPHSLDLPSYVFLLPSIFIFAPIIAFFYKKKAERPQIRSTGVTMLMTGSFIYYSLFVNLFASTILVPYFKASGGRIWFVSFIIYYLHLCVFIQYTLLSKCKLRPKLYLYLVSYPASTFFTAIILSLPIYFFTPFVESPLAQLVIALFPVALAGIGLYQTTRTLEPNKWTVKELTLRSMDEEEDQLIMKRKVKRVACGPPKDHEITDFKQPISIIQIADPHLGPMMSVDRLRSICENTVKLNPDLVLLTGDFFTVESFNPIDAIDRALEPLKELSGKTFACLGNHDYEEGCVELLEKALERIGCCLLVDKCVMAETRIGKVQILGFDYRHKARQEHITQVCAAYPPIPNVPRIGLLHDPGAFKFIPVDYQLAVFSGHTHGGQIGLNCLGINASIVGITGMPDHSLWQNGNNYLYVHSGQGCRSMMGTMVLRVGVPTEDSLLQVYFEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V338</accession>
    <name>A0A8J4V338_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 11">Alkaline phosphatase</fullName>
            <ecNumber evidence="2 11">3.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_002223</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Binds 1 Mg(2+) ion.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the alkaline phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.1" evidence="2 11"/>
    <dbReference type="EMBL" id="AJWJ01000059">
        <property type="protein sequence ID" value="KAF2076483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V338"/>
    <dbReference type="OrthoDB" id="7392499at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16012">
        <property type="entry name" value="ALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001952">
        <property type="entry name" value="Alkaline_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018299">
        <property type="entry name" value="Alkaline_phosphatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596:SF5">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00245">
        <property type="entry name" value="Alk_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00113">
        <property type="entry name" value="ALKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00098">
        <property type="entry name" value="alkPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00123">
        <property type="entry name" value="ALKALINE_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 9 11" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="8">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="460"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2076483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="528" mass="58721" checksum="7B64BB94485DA207" modified="2022-05-25" version="1">MSSNNNNNTNNNNVSRTPDNIGEDEEDIQINSETGMSHFLLEEDFSPKETKILRKYKKIFYLLAAFLTIVTIVLLIVFLLPRGWTPPKKLNVIMMIGDGMGPAAMTLARVAFNRSLVLDHYLIGTVRTYSFNSTVTDSAAGATAYASCIKTNNTYVGVDPWDKPAATLMEAAKKNGLKTGLVVTTRVSDATPAAYFSHSHSRYEEKFIITQLPDKDVDVVLGGGKKYFTDAIINSMSSNGYSTVYNKTDMNKVKKGKILGLFADGDIPFEVDRLYDQTSENIETPSLKEMSQKAIELLNQDNSKGFFLMLEGSKIDVCGHANDASGQIYETQAFDDAFQVALDFAKKDGHTIILVTADHETGGLTLGLQDRIDFYNEYYWNNTLITKVERSSLSMANMIIASNFTKTREIIQQYAKVEIDDSDLALLESLNSTSKYRYLQRAVGYVISRHAEIGWTTGGHTGVDVNLYAYAPDSLSENTKKEKISPLQDLFGNNNNIDICNFIKSKLNLDLESITKTLMSFSPFPPAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3E5</accession>
    <name>A0A8J4V3E5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Lysozyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008997</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000614">
        <property type="protein sequence ID" value="KAF2069689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3E5"/>
    <dbReference type="OrthoDB" id="17429at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052258">
        <property type="entry name" value="Diverse_Func_Domain-Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37612">
        <property type="entry name" value="FIBROIN HEAVY CHAIN FIB-H LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37612:SF20">
        <property type="entry name" value="PER-HEXAMER REPEAT PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035207588" description="Lysozyme" evidence="2">
        <location>
            <begin position="17"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="275" mass="27060" checksum="16511259ED2F2C9C" modified="2022-05-25" version="1">MKVLSILLLCILFVSAVYSGYSCSNPCYGDMCCSIPSNNQYYLTTFCDESTACGTGCSQLSLFSADSQRFGCGTTLTICSAGGGSTSGSSSTTSSGSGSGSGTTSSGSGSGSGSGSGTSGSTSSGSGSGSGSGSGSTSSGSGSGSGSGSSSSGSGSGSGSGSSNLLNEIVYGTCVKVTVIDAGPNISVEEEAGMPIIDASAQVCQTLFGSSSCGWSDSRLISAVQSTENDGFPVGNAFNVTLSQYQSIMKRGELLMSQCSLNSYDSKCKYNNHSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4L7</accession>
    <name>A0A8J4V4L7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chitin-binding type-4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_005017</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000190">
        <property type="protein sequence ID" value="KAF2073667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4L7"/>
    <dbReference type="OrthoDB" id="17911at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.50">
        <property type="entry name" value="chitin-binding protein cbp21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004302">
        <property type="entry name" value="Cellulose/chitin-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051024">
        <property type="entry name" value="GlcNAc_Chitin_IntDeg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34823:SF1">
        <property type="entry name" value="CHITIN-BINDING TYPE-4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34823">
        <property type="entry name" value="GLCNAC-BINDING PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03067">
        <property type="entry name" value="LPMO_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035256695" description="Chitin-binding type-4 domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-4" evidence="3">
        <location>
            <begin position="21"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2073667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="242" mass="28344" checksum="A1E7724BF861AD8B" modified="2022-05-25" version="1">MKIIYLFIILIISLYYVDARGYAVYPRSRQAFCADNRKNNFFYPLDGSGIKDPACRKAWRESGPKFVMRKNEYTVYVPHYKMGINALQAQVPRYLCSAGDVYKSGNSIPNIWTATVISVPYQSLEGERINYKYCETSITRNHESNLWEFYITEPGFNIDKQTLTWDDLIMFYNVTNILPEEQQETNEYCPTGTLYKIPITLPVRYERATLLVRYQSTDLRGKCEISCSDYLFKPILRKKLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V547</accession>
    <name>A0A8J4V547_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004426</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000156">
        <property type="protein sequence ID" value="KAF2074252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V547"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="589" mass="69847" checksum="06E98848E7FAB915" modified="2022-05-25" version="1">MSSTLSPLHLESKNFYGWYSSKDDHSFILMEKKERDVNLIEPKSFWLVFNDPFLNRSIWKNVRKLYVPFQKYENIHSMKWIFRNGSMQLLIDKVKRDEYLSDIPFVSNQLDQFKLINDLEFYSIFNRKYKRYLLSPQMENVNQFLDSILNNNNSNESSEDDIQNKSIFNKWFYSNTKNDNQDIKGPEEMSENELLEHYKLKMELNLFQDFRRALESKHFQIGKYVLDEWLYKACQPFGEDIRIGNKDILEYYCIKNKDNKKKLTISVSNLYHRDDFEECMKLLENSGNIDKNNPTHMNRFKTKRNLIYFLNNEYQYNNYIFYYISKGTMEQLCCVSMILESGGPRGAHYTLDRPEIFNRLYILVQQINLSMDELTKQFPDVAKEYTEYLNQYHLLYIKDFAPDDTTCYPSIVELSKIAKHGNIQTIKQIIESFIEWESFGIEAISSNTLTPFDYSGHTGITSYPPSTSIGALISGAASGNHLNVIKMIFHDYPRIREKFLLNHRQCINLLHHAIHHQNLEMLDYLLDQLNIVCPPSQGILNICQNNSIILSYFLSKPRKNNTPYVELEDFKNFYSRAKDCSVEFKLYQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V633</accession>
    <name>A0A8J4V633_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2 NT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_003251</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000100">
        <property type="protein sequence ID" value="KAF2075422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V633"/>
    <dbReference type="OrthoDB" id="21302at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019448">
        <property type="entry name" value="NT-C2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51840">
        <property type="entry name" value="C2_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="649"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="domain" description="C2 NT-type" evidence="4">
        <location>
            <begin position="9"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="61"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="152"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="341"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="436"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="537"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="342"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2075422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="674" mass="75108" checksum="0BDED67AEB842B32" modified="2022-05-25" version="1">MIKGIKGGLSHARKDKIKIQYDIELKSIINVCNDIVGQDISIKWERGKKFKGQTKECKVVKFQSPPTTAPTSPTSSSPSKTTPDTEKDPAGIVKNNPKIITSGVGSQQQHVGKKNDALAAPIPLVHSVSTGSIPTITPQKYDHSTLLTPNINYKSHSRNSSATGDSAHEASNISATTGSHSMLSRKQSKLLLGNRIDGTNSLLGGDGVVCLTNCDKITFRVTLFSSTTHSKQDYSLHPNYEQKLLYIYLLNKNNVLCHTSIDLSLHTEDHGSIVQVPFPSSSPNQPTLHMNIKTDFLKYNDKTIIKASPEMVSKKDILGSISPNGANKLIEIEGEVYIVDRNNNSSSNNNNSSTAPVTSGSSNNKNKIAKKIQENSNLPIPIISTSNKSNSEMEELAAHPTISDNSYSGQTTSWDDYTETEPISKTMDELNHNHLISMKNNEIKELNMKIKDLQEEINGNQRLHQSLLSVIEDCKSKVMEYEESVYRAQSLENFVKNQLILERFEFKKKIKQLATKYSSPNAPLSPIDSNPNTPTGLSLVKKELEEKNKIIVEKEKNIENITILLDSEKAEKEQIIVKHKQALQENEEHLKKLEKLKETIDKLAKESNDRNLIVENEARKKAAANHTVSKDKSFKDHLIEAAKQKYPSLLYNPVLAWSFAIFCFAMFVLKSFEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6J5</accession>
    <name>A0A8J4V6J5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PDZ GRASP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_005771</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000239">
        <property type="protein sequence ID" value="KAF2072914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6J5"/>
    <dbReference type="OrthoDB" id="3318at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000026">
        <property type="entry name" value="Golgi reassembly stacking protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007583">
        <property type="entry name" value="GRASP55_65"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024958">
        <property type="entry name" value="GRASP_PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12893">
        <property type="entry name" value="GOLGI REASSEMBLY STACKING PROTEIN GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12893:SF0">
        <property type="entry name" value="GRASP65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04495">
        <property type="entry name" value="GRASP55_65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51865">
        <property type="entry name" value="PDZ_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PDZ GRASP-type" evidence="7">
        <location>
            <begin position="20"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ GRASP-type" evidence="7">
        <location>
            <begin position="115"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="226"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="227"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="238"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="267"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="281"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR607583-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2072914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="322" mass="36181" checksum="B388380392CF91FC" modified="2022-05-25" version="1">MGQQQSQLNQIQEENPYNKTGYHVLQVHSNSPSSGKLYPFFDFIVAANQVVFEKEDQRFSEIFRNNIDKNVHLIVYNIKTDTTREVVITPSTTWGGQGLAGISIRYSSWEKTLETVWHVVDVYLNSPAHDAALETRTDFIVGTPDIIFNEQEDFFTLINNNMYRPIQLYVYSTLTENVRLVNITPNKNWGGSGSLGCDIGYGLLHRIPTKQIQTSQMISPSMERLKISNSPSTQPIANNSLTTPSTNISSPSPNVDYTAFPSPPTSQPIQINNTNNYLTPQKDQSSNNNNNDDGQQVNFDLTEQLHAVSNQSQHNIGIIPNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8G5</accession>
    <name>A0A8J4V8G5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Acyl-coenzyme A oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_003681</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the acyl-CoA oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000118">
        <property type="protein sequence ID" value="KAF2075029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8G5"/>
    <dbReference type="OrthoDB" id="538336at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003997">
        <property type="term" value="F:acyl-CoA oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033540">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA oxidase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000010">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000082">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000005">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055060">
        <property type="entry name" value="ACOX_C_alpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012258">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002655">
        <property type="entry name" value="Acyl-CoA_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909:SF226">
        <property type="entry name" value="ACYL-COENZYME A OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909">
        <property type="entry name" value="ELECTRON TRANSPORT OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01756">
        <property type="entry name" value="ACOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22924">
        <property type="entry name" value="ACOX_C_alpha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000168">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10 12" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="14">
        <location>
            <begin position="163"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase C-alpha1" evidence="15">
        <location>
            <begin position="316"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase C-terminal" evidence="13">
        <location>
            <begin position="539"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="448"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000168-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000168-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF22924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2075029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="656" mass="74005" checksum="C43E8AC62B5CF4E1" modified="2022-05-25" version="1">MDKSFIRRIDNTINHLISYNHGHDIEGLHGHPDSPPSAKSLRSMLDGEFHFDRDRLRELFQTSDSFDEVFLNETIDIDTHREIILKACKEIMNLSIVSLNDMQKNPKKFVSWFESISYCDPSVALKFAVQYNLWGGTILFLGTKKHHDKYLKGIENGTKLGVFGLTELGHGSNVNGLETTATFDSNTCEFIINSPTFTSQKYWPGNIAKHGQYATIFARLIIKGQDKGIHAFVVPIRSEPDSVNPYGVILPGVHIRDIGLKASYNGIDNGGLLFKNVRIPLDNMLDKFSHVDQKGVYHSTVPANRHFGLMMSVFFIGRVCLSVNSLCMMKVGLSIAVNYASNRAQFAKTTKTKEELPIINYTTTQRRLIPSIARAYAMDFSHKYICDFVGVKPLDVLHSYSSGFKAITSWEAVNSLQMCREVMGGQGFRMCNRVAMLRDHADVVTTGEGDNTVLTQQVGKFLLGKYQIAVSQSPPSFTGELSFINSIETSPVGQQLTSISYQQWLLEKREHELLKELHSRMYGSHRGGSLVQPNTEQWFHLWNQSLPLVIKLVKANVDRLAQNFFLQKIIDSSNVLTTDKATLTALKNLCRLDSLKQIQLDLGFFVAKGFITGEHKWIQIEDEINHLCSQIVPYIQILTEAFGVPLKFNPLEKNIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8N4</accession>
    <name>A0A8J4V8N4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_000711</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat PRL1/PRL2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000014">
        <property type="protein sequence ID" value="KAF2077987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8N4"/>
    <dbReference type="OrthoDB" id="10256122at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000012">
        <property type="entry name" value="Putative pleiotropic regulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045241">
        <property type="entry name" value="Prp46/PLRG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19923:SF0">
        <property type="entry name" value="PLEIOTROPIC REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19923">
        <property type="entry name" value="WD40 REPEAT PROTEINPRL1/PRL2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="182"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="224"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="266"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="308"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="391"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="89"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2077987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="496" mass="55176" checksum="DE55025464EED587" modified="2022-05-25" version="1">MVKKDQVTNGKINKNSNIEDDKINSLCKFSILNTFELYTPQTPISTATTPTNGANNNGDLSDESRKIKIYSKIKDEYEPVKDLPTQSFAKKAAATNNNKQKTTTDKPTIEEEDTKDIDNIINNLPSQKDEKRRKIDSNAISLYNHNVNKDTRGGSLALVNGYEPLQYTKPDWHAPWKLMRVVSGHTGWVRAIAVDASNQWFATGGGDNTIKVWDLASGELKVTLTGHVSQVRGLAISARHPYLFSVGDDAKVLCWDLECNKQIRSYYGHHNAVNAVALHPTLDILFSGGRDKDLRVWDMRTKAKIFEIRGHSDAITSIAAQNADPQVITGSNDQTVRLWDLASGTCAATLTNHKKSVRALAVHEKEFTFASASADNIKQWKCPDGTFIKNLSGHNAIVNALALNQDNVLVSAADNGSMQFWDWKTGHCFQKNQSIVQPGSLDSEAGIFAMSFDKTGTRLITCEADKTIKFYKEDENASEATHPVVDWRPSRDEKRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8Y8</accession>
    <name>A0A8J4V8Y8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">RNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_000462</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DEAD box helicase family. DDX47/RRP3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000008">
        <property type="protein sequence ID" value="KAF2078272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8Y8"/>
    <dbReference type="OrthoDB" id="10261904at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17954">
        <property type="entry name" value="DEADc_DDX47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044765">
        <property type="entry name" value="DDX47/Rrp3_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF20">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="14">
        <location>
            <begin position="122"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="153"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="351"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="515"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="9">
        <location>
            <begin position="122"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="103"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="515"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2078272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="550" mass="61266" checksum="034D90DD813AD07D" modified="2022-05-25" version="1">MVTKKQSSSSPSANSIATKKVTKTVTQSAQKDTATATVAKQQVAQQKKNNTVKKPVKEIIKQPPQEEDEEEQQEEQQEKNEKSKQPMKKAKFDEDDDEEEITESSTTGKGITNSGDKKIENVTFESLGVHPQIADACTRLGFKEPKEIQREVIPWAIKGRDIIGLAQTGSGKTAAFVIPVLQRLLDQPQAFFGLALAPTRELAFQIAEQFTALGSIIGVRCSVLVGGIDAMTQSISLAKKPHIVVGTPGRVIYHLENTKGFNLRSIKYFIMDEADRLFNQDFEEEINKILTVIPKDRNTFLFSATMTSKVAKLQRASLSNPVKVQVATKYQTVDTLLQQYCFIPHKYKDVYLTYILNELAGNLTIVFCSTCASSTKLALMLRNLGLGAIPINGDMDQTKRLASLNKFKQGQKSILVATDVAARGLDIPSVDLVINYDVPSNSKEYIHRVGRTARAGNSGRSITLVTQYDLEMYLRIEHVLEKKLDQFQAQEETVLILLERVNEALRLATNEIKDKGYQSKEGRSDDSENTTGTSIKKVNVVKKVQKKKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V9A2</accession>
    <name>A0A8J4V9A2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Complex 1 LYR protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000003">
        <property type="protein sequence ID" value="KAF2078542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V9A2"/>
    <dbReference type="OrthoDB" id="190541at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20251">
        <property type="entry name" value="Complex1_LYR_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47579">
        <property type="entry name" value="COMPLEX 1 LYR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47579:SF3">
        <property type="entry name" value="COMPLEX 1 LYR PROTEIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="103" mass="11831" checksum="89692290E64C14AD" modified="2022-05-25" version="1">MCTQTIVKSAASIKKNSVHIYRDCIRLGRYIGSMNGFTGNMLTQVRLTFRKNMYEVDPEKIEEQKGDALRFMTNFMQTEAMRIASQHKAMKSSQTARTKTTLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VAD5</accession>
    <name>A0A8J4VAD5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001656</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000041">
        <property type="protein sequence ID" value="KAF2077024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VAD5"/>
    <dbReference type="OrthoDB" id="6130531at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="100" mass="10584" checksum="7EDD9E0F9B8BD507" modified="2022-05-25" version="1">MCVYKEKNCTSSDLCQISSGCDKKTGQCILSPVQCNSTNKCETTACDQATGKCQVTKVKTCPPIKALLGLGILCQAQICDPPTGNCIKDPNSTFLSSLIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VAX0</accession>
    <name>A0A8J4VAX0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001134</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000025">
        <property type="protein sequence ID" value="KAF2077594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VAX0"/>
    <dbReference type="OrthoDB" id="24030at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35885:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35885">
        <property type="entry name" value="CARBOHYDRATE BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101908">
        <property type="entry name" value="Putative isomerase YbhE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035324988" evidence="1">
        <location>
            <begin position="21"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="317" mass="34809" checksum="C22C974D7D43E74D" modified="2022-05-25" version="1">MNKQLFCLFALLCCTTTIYADTYGVGIFNDGTGVAVGLIDYTSPGFSATTQLYLPNLNYIGNSSLSSPNTQVSSYNYATKQLTFLAYQPNNSTSPVIVTVDCNKWKVVSNVAAPVSYLAGFAYDQSNSFGQNMFTTFKKAEDGYLYVAKINPVNLQISNYDAFQGFYLTSATDDRGNYFLVYFNNFGYYIRIYNSAGQFAMQKTLTFGNAPSNMQSGPINMAYSTKDSNFMATFMLKNDDGSLSSTLAWMDWSTGTFNFTSILSNHGFTATANVADPNQNLVYIFGSNPSTSFMLTFDTSSNDLMAIGGYTQILAAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJ73</accession>
    <name>A0A8J4PJ73_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010412</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001095">
        <property type="protein sequence ID" value="KAF2068262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJ73"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053331">
        <property type="entry name" value="EGF-like_comC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24032:SF17">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24032">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035161011" evidence="2">
        <location>
            <begin position="23"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="556"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="564"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="606" mass="68676" checksum="7DA16B5C52631786" modified="2022-05-25" version="1">MKNYIKFGLLLFFCFQLHQFYAVELVQQDFNTFSNFANKLGINITKYNSSNICYTNGLFCFPFSSQYWIINFDWTGNNTYSIQHEDIQFPYMQRLRFEKMVLQDTFFGQLITKLPNVTEFACNSCNLISIPTGTSKVITLSLNSNDFNGIQKFSSIPQTVRYFSSYNSSAHESLVWVNDLKVAKVFESLSLSTNAFPDCSNLNVTELKLYLTNNFETSLANTKNGLSKTTKYVYLEGKYNQVQNLTFPNNFTTSQTFSKGDTYGLTTYWSLESVYVNWKVATPLVFNTPNSLRNIKIRFGSFSTNDGKFPIQSSSLLDTLEVSYSNNLVELDFNVLRKIRSNLFGSNKISMELPAEIKWENDDQIAWSINPNTNSIRSNYALDLRTNSFYGTLPKWTCNTYSIFSSNKFTGELPSCYTCFLKDSFTRSKISGNNFTNYQDSWTADKYPPCTTIQINQVYIQQGAYQSSRYLQLIGKDLGVHSTIVGMISNFNVSFTPLIENEWYQALVNETVYSLLTRSSTIQVYFPIPDKLITTRGNFKELVSYNTTISQTTFNVKSNGGGSTSTSTSGTSSSGSVSPTDEPNSCSSLFISQTLLLISLVLSLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJQ4</accession>
    <name>A0A8J4PJQ4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010326</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001040">
        <property type="protein sequence ID" value="KAF2068349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJQ4"/>
    <dbReference type="OrthoDB" id="439743at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="32955" checksum="667EDBC6984A0839" modified="2022-05-25" version="1">MNPDDSLEIPPQVQHVKLGRGLKHVLTAVPSTIKSIISPAPWSDNIVFGEYTEPHDTKSLLNSTANLHLHGIDIFNSFIVPQALGDNIKSISFGHFFNQVILPGTFPNSVEYLDFGYSFNQPLHKSLLPSSLQTLVLGRKFKQDLKDVFPSTLTQLNLHLLWVSVVQSISQFPPNLIKLSIPYYDGVLGIIPTTTTLVHLELNTVISNFIKSPGDADADIIPIELLPPNITTLILNQDMRIQSYDLIPSTIKKITLCDSIRQGTKIPITIESVVLPKSFNSPLDTILQTFDNCDPNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJZ9</accession>
    <name>A0A8J4PJZ9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009490</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000722">
        <property type="protein sequence ID" value="KAF2069190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJZ9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="82" mass="9446" checksum="27B7D922BE6299EF" modified="2022-05-25" version="1">MALYNQPISSDLFPESLTELKVNMFLPNPLIQCNLPHLTKLSCQYTKGILTITDIKFNSKDPKEHIFLLNCITQYNKTCSIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PK22</accession>
    <name>A0A8J4PK22_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010511</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001162">
        <property type="protein sequence ID" value="KAF2068163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PK22"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="128" mass="14263" checksum="E696212A9BB697B9" modified="2022-05-25" version="1">MLYSSLKASSMTVEDLDIVTQISVATTHQIGNCQGSVCDFHAVVQRMGSKSTTTAAISSALIHTDQNHIDQSQPLVHPKRSLQQYGTCRRPHPSPHQHYPNLILQLTRSLPPSYQKQSTYYYVSSHPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PK27</accession>
    <name>A0A8J4PK27_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_010496</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the csb family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001152">
        <property type="protein sequence ID" value="KAF2068178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PK27"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008601">
        <property type="entry name" value="Dicty_CAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16148:SF14">
        <property type="entry name" value="MYND-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16148">
        <property type="entry name" value="NF-KAPPA-B-REPRESSING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05720">
        <property type="entry name" value="Dicty_CAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="209"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="209"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="226"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="121"/>
            <end position="128"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2068178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="300" mass="33584" checksum="F525835EAD8CA953" modified="2022-05-25" version="1" fragment="single">MNQGAPISISIDSNGKHTINGNGCVLPIPMIFPPPCFLMISGPAKFEGDSEVWKGRKEVKNVVVEYRGKSFPFGDCRVEVINEDFDDDGINDYRIHLWPKTHVQNMPYYAKGKRIEDTVVGMDNAGKTTILKKFNGDISTISPTLGFNIQTLLNKDVPTPPIQQQQHNDFGDFDITAFKTQISHLNLNTHRHKFVNLWCRYTIISTPSTSTSTPITPSLGNKDNNKNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNILTPSTLTLRSKNKPTIAIIPTTTTTKEKEVLDFDNISTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKB7</accession>
    <name>A0A8J4PKB7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009935</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000859">
        <property type="protein sequence ID" value="KAF2068739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKB7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="103" mass="11623" checksum="0540247E1288DB77" modified="2022-05-25" version="1">MFRRVILTFRGCEEAVAMMRIVAPSMKLDDLSSVKDNEVVQSQGPLFISLSILQRTCTPEIPVLIFFGWAIRKSQNITPKSSGLIPNSCLYLETSSMSLNQYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKU5</accession>
    <name>A0A8J4PKU5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DSCP-N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009943</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000861">
        <property type="protein sequence ID" value="KAF2068733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKU5"/>
    <dbReference type="OrthoDB" id="3438930at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007643">
        <property type="entry name" value="Dict_spore_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04562">
        <property type="entry name" value="Dicty_spore_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DSCP-N" evidence="2">
        <location>
            <begin position="131"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="496"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="554" mass="62036" checksum="58C8925881893110" modified="2022-05-25" version="1" fragment="single">ERDNWRSDSERDNWRSDSDSDREDRRNKWHKAHPEVRRGGRNILGFSDFRSDSERDNWRSDSDSDREDRRNKWHKAHPNFPRRSDSDRDNFRSDSERDNFRSDSSSSDSDREDRRNKWHKAHPEHRPQRIDCESLSEHSCEKHNDQCIALPFVSCCGKTKKFCVRNTGNCLDKAELTCGRHDNGEIYEFWSRCTPRNNFSGFHQRNTTCEDLDCERHGAVCEYHQETKCLGTSCCQKVPRCVRKHHDNHHEQHLCGGERCHQGHYCATIHGRKRCIPYHHNQHTQGDYCGFDRCMPGQTCTVIQGRARCVWSNNNANKLCNGKPCFPGQFCSRIADQERCVWFPHGDGEKCGNTFCSEHNSCVMVGGVLQCLPKKTGNHRCGIFDCEYNEYCIMVNGLLQCNANNGTAPYACGQNTCNPPQVCVAVDGCDQCVDPPAPEKNCGTEHCGTAELCVAVGGSLHCVPKPANCGLDTCTGNEVCINVSGLQQCVAPITTGSASTTDGATTSAATTGASTTSPATTASRQLINKCGDRSCASNQICVSLTGHLTCVNFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKZ1</accession>
    <name>A0A8J4PKZ1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_009867</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000836">
        <property type="protein sequence ID" value="KAF2068808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKZ1"/>
    <dbReference type="OrthoDB" id="2430314at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34500:SF1">
        <property type="entry name" value="DDE TNP4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34500">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="3">
        <location>
            <begin position="122"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2068808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="355" mass="40903" checksum="C70B0370311547C6" modified="2022-05-25" version="1">MSTVKINNFEERTFKRNFGLSKQIAKKIYNKVPFNYCLPEKNFLMALDFICNYPTLHTLAQKYNLTERSIRDKIWMSIELISQFIDPPNINWRFLSEETPRCLKPGSGVNDYFLTAVDSTACKYASSDSSYFSFKHKEKVLKYQVLVCTRTGIICNIYGPEKGTVSDITLFRRSLKIPAYKLYQDPNEFIIGDKGYIGESDKILTPFKKYRGQPWTPNHELFNNYLSYHRRIVENTFSRYKKFKSLSVPWRSSPSKHSMVFKIVTYIINCHIMGDRIDKSPPTFIKFRPNEQQEEDEEIDGPPTTTATNATTVATAASVSIDVQQQKQQQHDVSLHSFPNVSLSPQIGSISAVSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL41</accession>
    <name>A0A8J4PL41_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Potassium channel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009662</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000769">
        <property type="protein sequence ID" value="KAF2069022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL41"/>
    <dbReference type="OrthoDB" id="73653at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016286">
        <property type="term" value="F:small conductance calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015449">
        <property type="entry name" value="K_chnl_Ca-activ_SK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10153">
        <property type="entry name" value="SMALL CONDUCTANCE CALCIUM-ACTIVATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="83"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="256"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="285"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="315"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="518"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="538" mass="62187" checksum="5F3478F5CE52F343" modified="2022-05-25" version="1">MITSISSSSIIPSSSIIPLPPPLPPPLPPPLSPLSLPTIHVPPSIDCCELSTTTTNEIKSKTNKRKLYNRNVRSEQTDRSLRNRYLLITVNGIMGLFLMVLELQTSWTGATLEINQASKIIRALIFILTLILVVQLMDYYRLLLSDVTHGWLTKKARGFRVTPINVLKSTTLAPLFWAEVIICMIQPMPFVSGIDVWWKDSKWGLLMWFRLYLFGRVLRDFSAIYQSRSAISKKFPISPKFNWGLSFKSILAKRPIATYLVITFTILLITGHILYIFEREVNPKFTFPIAFYISFLSMSTGWPTDTYDEYNPYTFIGRFGAILSCVLGLLLLSCLIDNFGRLTQPNSHQRPLLNHLTLIEVSKMEKDSAAKLIQCVWKRWRWRNSENSFSQNRDLFNSLEAQHCVKYIENVKTFERNRRYRKEIISYLKDNSSTPTPPDTSSSLEELKQMFEKKFTQDQIVKESLELKIKQLEDNQNLLTNQLSLLIQQQQQQQQLQFQQNQQLFYQQQQQQQLINNANNNVNNNNNNNNNNLNGADG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM81</accession>
    <name>A0A8J4PM81_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dickkopf N-terminal cysteine-rich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_009114</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000638">
        <property type="protein sequence ID" value="KAF2069573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM81"/>
    <dbReference type="OrthoDB" id="4405280at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030178">
        <property type="term" value="P:negative regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006796">
        <property type="entry name" value="Dickkopf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052326">
        <property type="entry name" value="Diff-Dev_Assoc_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33459">
        <property type="entry name" value="DD-GDCA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33459:SF7">
        <property type="entry name" value="DD-GDCA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04706">
        <property type="entry name" value="Dickkopf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035327879" description="Dickkopf N-terminal cysteine-rich domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="359"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Dickkopf N-terminal cysteine-rich" evidence="3">
        <location>
            <begin position="146"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2069573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="378" mass="41794" checksum="50BAF3EFA5AB81AF" modified="2022-05-25" version="1">MMRIFICVILLSTFVTLSLSQANNDCVFNKCIGAGGTCLNNVTRSETYQCEPGYRCNGDEFPTCIPWAKEGEYCDQDTFRCMNGYTCYQLGSTTRVPTCQMLNFKGYNETCVTDLECASPLKCKFPERQCTLPEKSSSQCSPNGAGCGFGQYCQYSNSTYKCASWKPTGEECSSNQECALDSICSVRPIGTGSVKVCNKQLNKRLGERCYPNPASDFTLSPTFDVIPRFDCNVTAKLYCGSDGICHTPTDSGTPCSATQPCLTQYDTCMCAKENNSTSSYCARNVNFTPECGINIQDLFKCAMDNQCVTVGSSLTRNSCVMKNCRDYICGNSCYTKQTQKDYSCGSDDYNYFCTNSSSLVRVSLLLLSIMMIFSLFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMW0</accession>
    <name>A0A8J4PMW0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-formylglutamate amidohydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008521</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000532">
        <property type="protein sequence ID" value="KAF2070164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMW0"/>
    <dbReference type="OrthoDB" id="71260at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.40">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="40959" checksum="4C2F7E34FC65D34C" modified="2022-05-25" version="1">MSNTSTILNNKIDKNVEVYDDEYIFYQQGNIPVILTFPHSGKLKLNGIPTRTKGVIDPDNFTDLLAIAIIQCFNCVFCCCSENSDCCNSDSKNSCCTNTNDDGDGKDNCCRQPTSTTKNTNCNTDCQCSCNNINFDSNKIDYQYGCPRPYIVYSKIHREMCDFNRNQDNAFEHPQMKQHYNLYHGTIDRFIKQIKENDSNWNKGRALLLDMHGQSDLVDYIVRGTRNTTTVTSMLNHFGNDSLIGPNSIFESLVQQNINVFPRTVPQEETTNNGVIDKVDSKSRFFSPKPALAKIELNPYKEHPYFSGAFTVGNYSSPPYSIDCIQIEVGSKWRSNQENRTTISKALRSSILTYYNSFMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN08</accession>
    <name>A0A8J4PN08_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007851</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000442">
        <property type="protein sequence ID" value="KAF2070827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN08"/>
    <dbReference type="OrthoDB" id="15899at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="174"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="23655" checksum="D7DB09F4B428CB9D" modified="2022-05-25" version="1">MSFFVVTNNNSTTTPTPSKSINSIEEDEQTLLIAKTASEESQQSKKPLLISILTVLISIPALVGSFGSILGLVVSGAAVTFAHAITFAITLAILTNLSQYHFWKCQTRTSDRGHFYKFGPFYLTAIAVPLCVFDVFRHLLVDNQIWTIHSFISPAAYRPGCNEENIKCLSVMGVFSAIIFTYTGYILLLTGTVWCADLHTKIRKAWRKLRPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN49</accession>
    <name>A0A8J4PN49_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_007708</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000426">
        <property type="protein sequence ID" value="KAF2070975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN49"/>
    <dbReference type="OrthoDB" id="6283463at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028307">
        <property type="entry name" value="Lin-54_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446:SF34">
        <property type="entry name" value="PROTEIN LIN-54 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446">
        <property type="entry name" value="TESMIN/TSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51634">
        <property type="entry name" value="CRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRC" evidence="5">
        <location>
            <begin position="263"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="102"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="186"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="395"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="517"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="102"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="186"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="400"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="521"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2070975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="576" mass="65046" checksum="1879D266DBE5BE66" modified="2022-05-25" version="1">MSRQNSTFKDDINSNNNSMMIDNQNPISLQDQIHIDVSMVIDNANLNSNQQQIQVDLQHIQQDLHQQQDLQQQQHESSLEPTIEQQQEANLNHIASSLSTPLIPQSQPVQQPQYQAPSNPPPPITRRSLANQNNMNKNNNNNINNNNNNNISPISSIENQIDKLQSRNSSSESSALSTLSNLSQIFNNNNNSNNNNTSTPLSTPPSSLSSSSNTLVGSTSSLTSSAPSSQNTTTTTTTTTTTSTPTTATPTPTTSQPPPVTNECRRCHCKNSKCLKMYCECFANRVLCNGCLCFGCMNNEANIEKVERARAQTLERNPDAFSIPIKKAQIRGCHCRRSHCLKKYCECFQANIQCTDNCKCIDCKNHSHPKDTDKSRRDDVPLNSQSLLKKAKISNNNGSIQSFPPQQQQQQQQIPLQIPIQTKNNVHNQQQLQQPQLHQYQQTINNNNYNNNSSIENSFLKQNLLKLNSFTDDLYKKLKLDQIKNLSLEFIKIIKDGKTGTSPIKVSLVCEEELDDFNSNSKDHNNRQKEEQERQEREEKERESNFVKREAGILTSFSTFLKNLIETDQDENNNEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN67</accession>
    <name>A0A8J4PN67_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">endopeptidase La</fullName>
            <ecNumber evidence="7">3.4.21.53</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_008567</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Hydrolysis of proteins in presence of ATP.</text>
            <dbReference type="EC" id="3.4.21.53"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase S16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.53" evidence="7"/>
    <dbReference type="EMBL" id="AJWJ01000540">
        <property type="protein sequence ID" value="KAF2070115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN67"/>
    <dbReference type="OrthoDB" id="2411602at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19500">
        <property type="entry name" value="RecA-like_Lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000021">
        <property type="entry name" value="Lon protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.5270:FF:000001">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.5270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004815">
        <property type="entry name" value="Lon_bac/euk-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054594">
        <property type="entry name" value="Lon_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00763">
        <property type="entry name" value="lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43718">
        <property type="entry name" value="LON PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43718:SF16">
        <property type="entry name" value="LON PROTEASE HOMOLOG, MITOCHONDRIAL 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22667">
        <property type="entry name" value="Lon_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 8" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="12">
        <location>
            <begin position="142"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="11">
        <location>
            <begin position="735"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="31"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="93"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="362"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="828"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="871"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2070115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="948" mass="107545" checksum="E3569978A18253D7" modified="2022-05-25" version="1">MFSATKSLSQLQKNRYIFQSLKQQQSNITSYNSFSSNNQKKNINNNSNTNNNSNHNNYNNHKSIFNYNQLFSSTSSSTSLIKDSKLNSLIHRVNYSKSSKPPKDEKEINENEEDKEKQFNDDDTIDSLSKEISLIDKSSNKKRDWPLNDEVLVFSIQSIPFSGAKGSLLLNQRFIDNMIPNIAGRTFIGLFLQKDGNKRDDPNSIDEIPRVGVLAHVSMTPVGHHYFEALKRIRIKRFITNTPPFCVEIEPYEPIETEDPKDPKIVATVEKITNLLERFDKQYPDAYRHNSLFFKQQREMDNPEIFISAATILYGLNYPAYCQEILETPSIVDRLEKIYQLLLQEEKLLKVQQEISVSIEEKKSNEQKKLLLNEQLKKIKSELGVELDEKEKTIQKFRDKLSKLNVPEAQKKVIEDEITKISTIEPSSVEFNMSRNYLEWLTSLPWGIFSQEFFDLTHSKQVLDCDHYGLNEIKQRILEFISVGHLKGTVQGKILCFIGPPGTGKTTIAKSIAKCLKREFYRFSVGGVNDEGEIKGHRRTYVGSMPGKLLQALKMTQTSNPVILIDEIDKMGKKYHSGDPSSALLEVLDSDLNSTFVDHFLDVPYDLSRVLFICTANSNNIPPALFDRMEIIPLAGYVEEEQYEIVKSFIIPKTLIESGLKVDQISISDSVIQSLVRFYTREVGIRELERMVEKIMRKTALMLVSGEAERVVVDQDNVHSFLGIPQYTSGRYYEHTPVGVVNGLAYTPKGGHSFYIETIAEEGKPVSGTPPRLRTTGKLGEVMLESSSIAYTFAKNFLYFIDPNNNFFETHNLHMHFPEGAIPKEGPSAGIAIVTSLLSLALNEPALNNLGMTGEVTITGKVLPIGGVKEKTIGAKRSSLKTIIFPATNRVHFEELASYVKEGIDVQYASDYKDVFVLAFPNKKDLLENAIQGLKENNNKQQQQQIQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNZ6</accession>
    <name>A0A8J4PNZ6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tyrosine aminotransferase</fullName>
            <ecNumber evidence="5">2.6.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">L-tyrosine:2-oxoglutarate aminotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">CYY_007231</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-tyrosine + 2-oxoglutarate = 3-(4-hydroxyphenyl)pyruvate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:15093"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:36242"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="EC" id="2.6.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14 15">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 2/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.5" evidence="5"/>
    <dbReference type="EMBL" id="AJWJ01000373">
        <property type="protein sequence ID" value="KAF2071457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNZ6"/>
    <dbReference type="OrthoDB" id="7042322at2759"/>
    <dbReference type="UniPathway" id="UPA00139">
        <property type="reaction ID" value="UER00338"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004838">
        <property type="term" value="F:L-tyrosine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006572">
        <property type="term" value="P:L-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000048">
        <property type="entry name" value="tyrosine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005958">
        <property type="entry name" value="TyrNic_aminoTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005957">
        <property type="entry name" value="Tyrosine_aminoTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01264">
        <property type="entry name" value="tyr_amTase_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01265">
        <property type="entry name" value="tyr_nico_aTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45744">
        <property type="entry name" value="TYROSINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45744:SF2">
        <property type="entry name" value="TYROSINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000517">
        <property type="entry name" value="Tyr_transaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00753">
        <property type="entry name" value="ACCSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="11" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="10 14" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0828">Tyrosine catabolism</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="17">
        <location>
            <begin position="40"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="15">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR000517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000517-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF2071457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="416" mass="45735" checksum="E8A056BC256C9BB5" modified="2022-05-25" version="1">MQNESTKKGWNIQPSKSATSTTNPIRELVDSGKYKPNPNKDMIPLSIGDPCVYGNLNVVQAVNDELIKNINSGKFNGYPPSIGYPAAREAIAKFCETPTSPLTTQDVVLASGASGAIEIALCALLNPGDNILLPKPGFSLYECICKNKGFGIKYYNLLPSKSWEADIEHLSSLIDDKTKVILINNPSNPCGSVYSKEHLNQILKVAEDNYLPIISDEIYAGMTFGDAQFYPIASLTTTVPILSIGGIAKRFLVPGWRVGWICIHDRNNIFAANNIQGAITVLSQIILGPNSLVQSILPTILDKNNQVIQKFFKDTNQTLEKHSNLTVDVLSKVSGLNPITSSGTMYQMVGIDSSKFKDISDDREFAIKLLNEESVFVLPGSLFGMNNFFRIVFCAPADKLEDAYQRIKTFCENHQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPB6</accession>
    <name>A0A8J4PPB6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">USP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006945</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000343">
        <property type="protein sequence ID" value="KAF2071740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPB6"/>
    <dbReference type="OrthoDB" id="21432at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02257">
        <property type="entry name" value="Peptidase_C19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF915">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="2">
        <location>
            <begin position="286"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="425"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="630"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="685"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="169"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="633"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="685"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="701"/>
            <end position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="784" mass="87338" checksum="8B0CF21B12A6AEF3" modified="2022-05-25" version="1">MNIGDTTTTTSTIEQQPTYNLFFDTPTENRDTKGFIFQHCTLTIESNNENKACLVIDPSPPSTTTTTASAAATAKEIIVLQNTIKNLTLVGKTIDVFFKDCNKLTITVDNMDPNGIDNIGKLFKKLCIYKKTNTLSGSGSILSATENTVIKGLYNIPYKPPSKPPPKPTTTTTTTTTTTPTTTTAAANSNGENDNNRLKIESKFNKSKDLPGFPTPTIKYSFTDFLTKKDIDIELKRKSPWDMSQQEKRIKYDSVDSNISAKQSERSISSRSTNTMPFPTPIKTRYGICNLGNSCYMSVVLQSMFGLTDLFRDIKNEKFKQYIPRDGVYNSVIKLYDKSNRFPKKKTSLLSSDIETGVDPSNFKDRVDKVCTYFQGFLQHDAHEFLVSLLDILEEELKNNITQMIKDEKVQLKIKDGKLQLSFNEKDKLNSDNESDENSSISDYDDENGASGSAIVNIKKASSTTTTSTSTSTSTTATTNCKDKEKEEKKQIKDFVEQICPITKHFKSTLINTFKCCNCHQISTSSEIFTDLSLDLPKSSDSQKKPTLSNLLETYFKEETIDRKCTHCRHTKSILTKHISKSSDVLAFHLKRFKMNSTSFLKNSVYILNSESIDIGSYMKPKYIEPIIDQSSDDSSISSSSSSSSIGSSISGNSSSSTSSIGSSNNIDIGDEILDFGMAYASASTTSETTTAIERNQQQQPKEKEEKEEKKSSKSQDDVEKPVIYSINTIVKHTGTLNSGHYVCYGNDKTSSKWTLYDDSDVKDVGQDQAINSNAYILFYTLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPN4</accession>
    <name>A0A8J4PPN4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008954</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000606">
        <property type="protein sequence ID" value="KAF2069724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPN4"/>
    <dbReference type="OrthoDB" id="20932at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.980.20:FF:000011">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.980.20">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="213"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="369"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="300"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="228"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="384"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="434" mass="50261" checksum="CC14EC32969DF68E" modified="2022-05-25" version="1">MYKNNAPEFLLTPYNNIYFNNESNLPVPSSSHLPHKHHQYIISSSMQQQQHMYNNSNIYPSYQQCNIVPNQDLNNNSNRSSSPVPNSPPAPQICTLTKCPLCQRGQPPLLLKTPTWASIMRVVFYSLHQEYPEKEYFSLKTDVYDYMTSHWEKLCISRKRSDNWRKQVQDMLSHSKNVFESGLEKVKQNGFWRLKYNSDPWIIAKENDLNNSSNGIFSKKRGNDEMEQQQQPQQQQQESSKRYRVDEEYNNNTVSSPMEDRLIKEIKCMREEVGQFYKLLPSMKEKIKYDNEQTLENINANQLREELSSLQSKLFLIESRLKKIEEKEKSFNVSNLICANETPITQQSIILTTPLVPSMSNNRKHLITSTPQFKLPSPGYSPISSPQTFSTSAPSSPSSATSSPIVRPISFPQLNPCKEFNREYLSPLVPINNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPQ1</accession>
    <name>A0A8J4PPQ1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_006725</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetyltransferase family. ECO subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000323">
        <property type="protein sequence ID" value="KAF2071950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPQ1"/>
    <dbReference type="OrthoDB" id="428854at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061733">
        <property type="term" value="F:protein-lysine-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028005">
        <property type="entry name" value="AcTrfase_ESCO_Znf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028009">
        <property type="entry name" value="ESCO_Acetyltransf_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45884">
        <property type="entry name" value="N-ACETYLTRANSFERASE ECO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45884:SF2">
        <property type="entry name" value="N-ACETYLTRANSFERASE ECO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13880">
        <property type="entry name" value="Acetyltransf_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13878">
        <property type="entry name" value="zf-C2H2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="N-acetyltransferase ESCO zinc-finger" evidence="11">
        <location>
            <begin position="191"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase ESCO acetyl-transferase" evidence="12">
        <location>
            <begin position="348"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2071950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="420" mass="47961" checksum="B6FA97DEC76C578C" modified="2022-05-25" version="1">MTDTLPTINNNNNGTTNSIIGCKRKSSSISSKNSTPSQSGFMITSFSMFVKKDDSSNNNKNNTIQKKEQQLNDDYQNEKRIKLDDVAVIDHHQDELSNFNNDEQKVPDNKFEKIKETTPIKSIIESKNNNNNNNNNDIKKEIIQKKNFKEWDWKSASKKESQHINFLESSVIIKKKAIGTKKVKVQDGLEQTVIDAGQKNVGQIVCNKCKMLYAHGTKEDEQVHKAFCKNIQSQKVFIKNWSNFKVINRFSNDDVIIMVSIDQDTSISSKLKAIKNMVDLDLGYYNNNNGNESKKKQEQVEEQDEKIFLYLDCNSKVLGVLICDRYVEKGYRIESASPVIQCTNTPTSVRCGVSRIWCLPSKRKNGIATKLMESMRTNAFYGYQLKMNEIAVTQPTSSGLSFFSKFFNTSNFLLYKINSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPR2</accession>
    <name>A0A8J4PPR2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F5/8 type C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000397">
        <property type="protein sequence ID" value="KAF2071243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPR2"/>
    <dbReference type="OrthoDB" id="10254111at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37916">
        <property type="entry name" value="CHITIN-BINDING TYPE-4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37916:SF2">
        <property type="entry name" value="CHITIN-BINDING TYPE-4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035296311" description="F5/8 type C domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="163" mass="17958" checksum="7B89F96A757785BA" modified="2022-05-25" version="1">MKVVNSIFVFLLLVGIAYSHLCLFSPQQRGSGPPLITGPGSDDCFNPVRQSNACGSKNAGAVVATLGVNSYYSVHFQQNFNHWMKSNPGFMDISLSYDGQTFKQLEVIPDFNAFDMWTQTNFTIPILVPSTPCDSCVIRVRYVPNNPLEPPMGFVQCADVQIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPX0</accession>
    <name>A0A8J4PPX0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006906</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000339">
        <property type="protein sequence ID" value="KAF2071778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPX0"/>
    <dbReference type="OrthoDB" id="19587at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040430">
        <property type="entry name" value="CudA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38092:SF6">
        <property type="entry name" value="C2 NT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38092">
        <property type="entry name" value="REGULATOR CUDA, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="407" mass="45538" checksum="AAC0A3CCFB23A7A3" modified="2022-05-25" version="1">MGHNQNFNNNCSTPSQSSSNNKTKISPYLQVCEQECISTENIILNNRNCNLHIVVKNTSFVLKIKTNDMSQVNFAACNVKAGLYYASDPLKEVSFIQNPPITYVGSACNNGEVFSIDTKISILSSQHQGNLFYIMVTCVPENNKTQVHTVLSFPIRVVSKVDHIKKDPNGVCEKKQTFIDILTERLNVLENYHVSQSQLITNLLKQKKKGSTSMAHGSSDPNEYSEPELLPGTSSSALSGAFSVSPPSSPFSISGSGSTSNKKQKASSQSKNKNTAERFLECFNRVIKVYKDEGYDKKAPELSDFLETFTEDEKYILVDLLDSFTFDESSQKKQLQQQQSQQQYNGAEQIDDFYGINSTGIQEENCTCESCPYRQQAEQFNLMLSPVMKFQSPMIKSMTPFSTSQFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ89</accession>
    <name>A0A8J4PQ89_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006487</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000302">
        <property type="protein sequence ID" value="KAF2072190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ89"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2072190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="77" mass="8033" checksum="908DDE6444F4780C" modified="2022-05-25" version="1">MIFKALSNISNPLKSLNMSSSQTSSGSVVVAGVSGSSSNACWYIIAIGGPTIWVEDNASPHKYYFGYPPSTSASTIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQU7</accession>
    <name>A0A8J4PQU7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_006548</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000308">
        <property type="protein sequence ID" value="KAF2072129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQU7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827:SF85">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827">
        <property type="entry name" value="RETICULOCALBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="38"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="78"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="114"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2072129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="149" mass="17079" checksum="794479168EC270C0" modified="2022-05-25" version="1">MGRLRDSIKFLDSCDFSGDKAIDKQELSRVLREKKCENVDLVVEYIFKLCDKNGDNKLIASEIQKNVSRLKQYTSNEDIAKKCAILLAKADSNGDQKLNEKELEKYLSENGEDFPSLEAFVILDEFDVNKNGFLEMGELRKYVKGKVEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQY1</accession>
    <name>A0A8J4PQY1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000360">
        <property type="protein sequence ID" value="KAF2071587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQY1"/>
    <dbReference type="OrthoDB" id="1263307at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052897">
        <property type="entry name" value="Sec-Metab_Biosynth_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37017">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37017:SF11">
        <property type="entry name" value="ESTERASE_LIPASE_THIOESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="12"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="236" mass="26206" checksum="14D19C2077994418" modified="2022-05-25" version="1">MEPIINKEKLTIVLCHGSWTGALVWQSVIPLLVKEGYKVVAADYPTRKFSEDVEYAKNLISNQEGNVLLVGYSYGGAVATEAGNHEKVVGIVYICGFALDTNESLGSVLNKREDLAKDGRVLDSQGFLWVKHEDFHRNICPDISKEDALAMSVCQKPLHLDVVLNEMGPNPAWKTKPCYYQVSNNDRMIPKETQLEIVANIKPKKTIHLDANHATISSHPKEVSDFILDAAVAFNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR27</accession>
    <name>A0A8J4PR27_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MIT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007365</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000388">
        <property type="protein sequence ID" value="KAF2071315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR27"/>
    <dbReference type="OrthoDB" id="16086at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02656">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04212">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00745">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIT" evidence="2">
        <location>
            <begin position="2"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="MIT" evidence="2">
        <location>
            <begin position="98"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="MIT" evidence="2">
        <location>
            <begin position="228"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="352" mass="39565" checksum="070C77077BE184EF" modified="2022-05-25" version="1">MSALQNALGIVKKAVDLDNQGKSQEAISMYSLAIDHLQQVATTNDTKTRDLIQSKIVEYQSRVQFLKSTLHLNSPNNINSSSNSFPSPPPLQQYNNNNKSLVDVAVEYANLAVNSESSNQIQNAIMYYKKCADVLEQASKETDDIETRLMLNQKKQEYNARADSLSSSSSSSFVNSNSNSNSNSNLPFQVDDPNLFNSNTNSSGNNLFLNNSNGIQQPQQYIELSNNSTEFVDMAINYATEAIEQDDHLNYEKAIHFYDLSVQYFTAALQHESNQQIRQLILDKSTNSRIRSTFLKNKIQYNGGNNSNSEFSSIPMALQPGQKYKDPVRKKTVFEKLSKTIKGPKNISDHWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR70</accession>
    <name>A0A8J4PR70_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000365">
        <property type="protein sequence ID" value="KAF2071526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR70"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="392"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="48"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="587" mass="67367" checksum="82C70DBAB558713B" modified="2022-05-25" version="1">MKKNQNLSSPTAQVEIKDGVNQETTNKTEKDEKLKVKSPNSMSKKKAPSSTPTNTPKSNSKKQAPSTPITTPATSKVKNKTPKSSEKSLTTFSPTSDQEKDLKKLNKLVSQYDNDIDTLIQEVGKLKTKFVLYHFRGISYYHPEWDKTINKVDHSIKKQEEPFYSRNLLQYFKDEEVPLEKKEQLANNHLESMSKLRKICAPMLSSASETIFPNKMHYLASNYVNNYVKFSKVMEKEYTTYGFPNNKNPFVSTSSLPTHSIAYAFGSKQYDSLEYNEIESFDKDNICGMVYCFQHSIQEYLKNRPYCPRVEVADGKISVGNIILSEYEESFLSFIPSSALSEQFVCTFPNLFCEGDIITPDQGYVFTQIELATFKNNVNYFKQFLQQEQQNSKDGNSALPQQDQLESPTDSVPQQNKQNNKINALKPIFEDLVKFKERFIKSKISEKNEPIIYLEIDGTFNFKLQQTLTNTKGKKKEQGRSRAKSFNLLRESHYLYHLSTLKQVEEVQDVSIPIEPKNFIEAVIDFYSLSPKELEELQNFISKKINNHKTTTTTNTTFNAVEDHSDSDDGDESNKNLCEELELLALE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRB1</accession>
    <name>A0A8J4PRB1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Prostamide/prostaglandin F synthase</fullName>
            <ecNumber evidence="10">1.11.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Peroxiredoxin-like 2B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_007196</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the reduction of prostaglandin-ethanolamide H(2) (prostamide H(2)) to prostamide F(2alpha) with NADPH as proton donor. Also able to reduce prostaglandin H(2) to prostaglandin F(2alpha).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>prostaglandin H2 + [thioredoxin]-dithiol = prostaglandin F2alpha + [thioredoxin]-disulfide</text>
            <dbReference type="Rhea" id="RHEA:28214"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57404"/>
            <dbReference type="ChEBI" id="CHEBI:57405"/>
            <dbReference type="EC" id="1.11.1.20"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>prostamide F2alpha + [thioredoxin]-disulfide = prostamide H2 + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:26373"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:53081"/>
            <dbReference type="ChEBI" id="CHEBI:53082"/>
            <dbReference type="EC" id="1.11.1.20"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peroxiredoxin-like PRXL2 family. Prostamide/prostaglandin F synthase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.20" evidence="10"/>
    <dbReference type="EMBL" id="AJWJ01000370">
        <property type="protein sequence ID" value="KAF2071480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRB1"/>
    <dbReference type="OrthoDB" id="40334at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02970">
        <property type="entry name" value="PRX_like2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000243">
        <property type="entry name" value="Prostamide/prostaglandin F synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032801">
        <property type="entry name" value="PXL2A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28630:SF3">
        <property type="entry name" value="PEROXIREDOXIN-LIKE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13911">
        <property type="entry name" value="AhpC-TSA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="18"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="159"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="66"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="189"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="206"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="229"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="246"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="267"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="283"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2071480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="697" mass="79533" checksum="170E0357255095B0" modified="2022-05-25" version="1">MNDRGLIYIFRDSSSITKSRYQTTQEKQQEKQQHNEKKNNRDIISAKSQQKNSNSRVKDIEKIIDSSDDDYDDDDDYDDYKYYQSSHNVQFNNHGDNNYRRIKQVIENVYNSNSSDDSDSDDNVIYIKDEESIRDEYSNQQTNEEDQDMLNPIKNKIFKHKKQKKESIPTTTQTQTQTPTTTKTNTIESKNKKPTFEKNEKSIGVHQVEPCQQQQQQQHHGQYHEQDQLQPCQQGEPNSTESTPPNESIISEVNNNNKLSSPEMESSEEKYYKQHPMEDDDSSVYTSPTSSTSSIISSTPTSTVNTPAPSPSSSPMYLTLSSADSPLPSLSSILGPISFSLGSAEIENADIPIDIVLKGIVVEDLDGNECLLTDLWAKKRCVIAVFRRFGCLVCRLQALDLSSLKPKFDQLGIQLIGIGFDPTGLAEFIHGQFFQGTIYLDRNRAVHRSLCLKRMGFWDSAVGFTDPRLTVYRKRAKDIGLPHNFKGDGMQLGATIVVGPQPQGAHYDFRQRGFTDIFDLNQILKACKSPLPEKISPQEKRYKLPPPINLPCSPIHFNLEIKPPKMVDTNNNDNNHSNHNRKPIVYHFGNKEYHLKAKEHQPQPQSSQQQEIDPQLLQKVEQLSNQLLSTGIKSFDEVFNNPTIGNYIKNVHISNSINNNNNNNLAKNKNEKKRKLSAHSKLWMKLEPETDLIYLPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRD5</accession>
    <name>A0A8J4PRD5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007795</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000435">
        <property type="protein sequence ID" value="KAF2070894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRD5"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="2">
        <location>
            <begin position="2"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="199"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="322" mass="37379" checksum="23DB24EDC3392053" modified="2022-05-25" version="1">MYGNPGCGKSTLLSTLIKRPGEFPSGISHITGLTKERKFITVDNVMYMDTPGLADANNRDFAAKEIEHSLKTNQRYKLFFVVSPTAGRISPVDFATLNIIIQSIPKYIKYGIIYNKLSKTIYKNYITKSDQLDALHESLLDRPPHKYLLLGNLDELEEEDNVIPTDQEFNDSLRNFVDQFPENHLPNHLPEEEVKPLPADQYEIQLKEMEESYKKALKDYESQIKELQDKYNNSCIRYETQTEERSSPFEREGPSSTRIKWRGLKTRKVKETKIERGEIIEKYLRINCIKGNGQTQYGEWNFIGTRDVITHIETKSINKKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSL8</accession>
    <name>A0A8J4PSL8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">NDT80/PhoG-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_005928</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000250">
        <property type="protein sequence ID" value="KAF2072762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="27041at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016540">
        <property type="term" value="P:protein autoprocessing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1390">
        <property type="entry name" value="NDT80 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051577">
        <property type="entry name" value="MRF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024061">
        <property type="entry name" value="NDT80_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037141">
        <property type="entry name" value="NDT80_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030392">
        <property type="entry name" value="S74_ICA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13029">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13029:SF19">
        <property type="entry name" value="NDT80 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05224">
        <property type="entry name" value="NDT80_PhoG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13884">
        <property type="entry name" value="Peptidase_S74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51688">
        <property type="entry name" value="ICA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51517">
        <property type="entry name" value="NDT80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1125"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1158"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1184"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="domain" description="NDT80" evidence="9">
        <location>
            <begin position="408"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S74" evidence="10">
        <location>
            <begin position="951"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="NDT80" evidence="6">
        <location>
            <begin position="408"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="332"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="681"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="776"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1242"/>
            <end position="1269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1324"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="334"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="404"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="695"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="706"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="742"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="795"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1339"/>
            <end position="1372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2072762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1386" mass="155379" checksum="53B04C1EB8E3AFFB" modified="2022-05-25" version="1">MTINNRNGSISINSNINNNKTSLTTNDIINNNNNLNNRASNNHLLENTTNSHHNIFPHPSHHNHHITTHHNNNTVNINSNNNNNNNNNNNNSNNNLVLNNNKNCQNTISNILGYNSNNSNNSSTTDINNINHYSNQNQNQNQNHSYINSGGSVNNNFSAPIPSNPFSTPNSNITNNISDSRKSPETTFIQQQQQFAIPTEYNSTHNNNASSSEFQKPFKSYINTNNHNNHNNQYINTKIYKRSNMHDPTMKNSILMGSSPLGMTFGSPNTPNTPNIINVNQPNNGNILSTSSSMNINQVINNSANFTNQQQNNINNTNLSFSYESLFNNLFDPTKSSQDFNSDFSNQHHQQQPQHHHHQQQPQQHPTDSYGLSLTTTTTTTTSNGQHIITPSSDVSSPSPPPSLSSIPASSPAPISPLQSPSSSNFSSLSPMSPLVSGSPNNASSLSKKSLKRAYEQAVQRDEWETYIPPTNAYYLFDSQKNNLGGTYDFKVKRTDKNIQYSELDAAWILYRQNRFQVDCEVMGSLEHWSNNESSNVLYVNNQENLVSEISGLYFTLYVLKFPNSSTIVCPNDSTERVPIHHLGGPSGKKEGRLPVEPCSVSKGKGNWSRLQFGSATANNARVNPDQPNPNQQFFRVVLTLNAVIDPNYQPPPPQQNAAFQFTSPVLSSSAQFFPLQSKISPPMIVRGQNPGRFLNHDKSSKKDSSSSPNNGKNSNNNNNNSNGNNSSGSHNNNNSFEHQQHQGAPNQNQNNLFSDSSDDYQHNNQQHQIQILYNTDQNNNNNNNSADKITGLRSSSTTSLGENNNSNNNLLSNNGIFHTNWDNSIFNQDNNNSTNFNLQIDQSNNLFQDQHIQQQQQQQITTYPLIPQNPNNLNQILDQSNLVSVPIVLPEPPPTEEPKYTNPRKSWRKNPYADDIIYTERKVGINTTTPSQALAVNGNVLITGELFKPSDQRIKTNIQKDKSDHWEKINKLKIYEYDRKKMHGYDDGNVGVDSVGRNSNVVKEKGFLAQEVKSVIPNAVKVAGEVKLQDGSTIPNLLVVNDRVLLLENIGATQQIGRTLEKEQHHIIEMDKEINRVKIEGKRDQHVLLTKMQDLVSFMHSEQTNHINPDESCVYCSLMGLGPAWTMFIFGFFIPIFWIVGSFFLFSPSRVKWMSGLANFIATIVGTTYVFLIIFYVPKIAAIIIIPVFAISGILICLLIGFFRQRNTESKKRYLRERMKLMQADGYNRLSDHVVNFRNDYNRSKKNRKNKATTSDDENPPLYKTQSPDVLYDSDDEIIIIDNHDGTGPKIRHGVDISKEFRKSSANNHKSLQELPLKDIVHNFGTKNGNQRKKKTTKSTTSLGSKIGSTPDTSKTFTSSTTTTTTTTTSTNIPPPIEKHASKVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTD2</accession>
    <name>A0A8J4PTD2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">W2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_004442</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000156">
        <property type="protein sequence ID" value="KAF2074268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTD2"/>
    <dbReference type="OrthoDB" id="10250831at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071074">
        <property type="term" value="F:eukaryotic initiation factor eIF2 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005092">
        <property type="term" value="F:GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11561">
        <property type="entry name" value="W2_eIF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.350:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.170:FF:000004">
        <property type="entry name" value="Eukaryotic translation initiation factor eif-5, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003307">
        <property type="entry name" value="W2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF7">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02020">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00515">
        <property type="entry name" value="eIF5C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="Translation initiation factor 2 beta, aIF2beta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51363">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="W2" evidence="7">
        <location>
            <begin position="202"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="122"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="124"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="142"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2074268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="362" mass="40517" checksum="A6C84B7EBD031999" modified="2022-05-25" version="1">MEVLQAKVEGKGNGIKTVIVNLQQIAKDLDRPPEYITKYFEIELNTKSNFESDRYSVNGQHSQERLSQILDGFIGKFVLCSFCKNPETKSLIKNSKIDLKCAACGRRSPLDMKAKLSSYILKNPPKESSSKSTHDEAPQAPSMKEASSASSSSSSKRSKKKKGEDDDEEEVVWLTDTSEKAAEERKRKAIGDSTSAVLSMVDIGDKDPVSYLYEFISKGPTDDAFMEELNKIQEEFGLRSYATARSAIEALGNKTTENTVKMVKNKNSLLQKIAKRRDGKLGICLGLEELCAKDETLLKSIQGILKNLYDADILTEEIIIKWHTSKAKSEAVIKATAKFVEWLQTAEEEEDEDDEENEEDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTI1</accession>
    <name>A0A8J4PTI1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009532</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000733">
        <property type="protein sequence ID" value="KAF2069146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTI1"/>
    <dbReference type="OrthoDB" id="22524at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1213" mass="137398" checksum="98D511D05B71F963" modified="2022-05-25" version="1">MYSKDNTGPRNDVATGSRINRKELALNVFQKIILNNNTLVPTNDKSFRDRVGKWSIKKEFRCFSNTEYGIVVLDLFAPDLVSLLSSHDGGQICKHINLTLALNRDYSVQYDNSIIEIISLLKDSIRSIRIKVYDNITAVQVQGETCISNLFNRVRDIIEKTTLDLLESYSDVEIVKEIFEIQDSDVRVEKSVKIGSGDVSIPSDTFHVVWNREEAPARGFIPDGVKRVTFSNPLQQPVGDALPESVGYVKVHDFNGCLSEIKFPSALKYLKFAGEFGAFVFRNADIPTGVTHLEFGVTKNIAYHWLTYKGTLPRNITHLKITDGKEYVKHRLAHPFLVPSSVQCIDINISLFNTLNIKMIDEKDYYCDADLSRESFTCFDANAIEMSPDYPLSITAASFSRHLQSLDVANYKKPFNANDLPPSLTSLVCLSTEQIPDHAQLCYLKYKISESIHPTVNTYELVFKSDERAITKPSIPAKVDSLTGSFTPKPAIPIAELTTPLNNYYAALVIPRNAVYKKVVARLPIQLPEFVEVYESSLHLPLNYPKSLHTVILTERLPFTLKYIPATVKHLYCCLDQDLMSSISTPIEDKYSFSNSNRSNNNNIIVSIDTFFKIWRNNYLVNNIKRFKVQPRFVKFQWTRTKGQTTHQFSEQEDIDYYAGSTTCLKLKVNEISTIPHGVTNHAIFQMLSADKQHLLLQLPRSIYKLKIYCSSLKKDVLPPNVKHLVLRVAEIKSSFHIPPTVEILELKLYDVKFEALKKWIPATVKRVAVTFASFEIDCFKHDTLALYNSRSLFGKGQVYEIYNGQEPIPISPTTTVLFWTSNTLILPGTIPMGVKRIVFGDSFNQAIMPGTIPSSVTEINFGQNFNQSFSLVHLPVSLKYLSLVRYKQPLQPHTLPPCLVQFKIQEDYQYYTTLIHLPKSIKYAKVRGVKLIQDHDNNDSTTSIQFKSIYSNLENSRQEAIISDINESSVEYQDGSRSRMESLSLNNPETEIHLSIDQNIAIASGTLDHLNIKSMVFKKDYNQSLPKGSIPNSVTSLVFAKGCSFMQQLICVPPSLCVLNLSHIYQSLTPIEFPLSLTELHLPAKFTQPIKKGNLPMGLKQLYVSQYFVQTLESDMIPETVQHLSLYEPKVVSKIPPTVQELSIYNSISLATIPKSITKLNFYDNAYLGNMDQLPCSIRSLSLPSTLFSGRIPPTVNHIEIKNYNRYLSIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTN2</accession>
    <name>A0A8J4PTN2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009445</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000710">
        <property type="protein sequence ID" value="KAF2069236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTN2"/>
    <dbReference type="OrthoDB" id="20557at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31494:SF4">
        <property type="entry name" value="THH1_TOM1_TOM3 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31494">
        <property type="entry name" value="THH1_TOM1_TOM3 DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="235" mass="26450" checksum="EF4812CF8CE9D663" modified="2022-05-25" version="1">MYSFFVSDEIRIQKVRGILVMVYIFGFILLCYQIPITIVSLKKDISLIEGIGFLILIFVYATGVFINGSVLLKSLKNHKKKSQYKHFNVMILKTKVLLVTLVTEFLVIVVHDLVFNFAIDQKYNNFPISTLITGIVDLGQMIVVMFVLANGKFYKYFKFQRVRSPVSYSTSDGASGESKNSGSKGTNISKSFDSKSIDASIDLSVSVYNNNSKSKFEQNDSRIIVSDSNPNENNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTV7</accession>
    <name>A0A8J4PTV7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_004912</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000183">
        <property type="protein sequence ID" value="KAF2073778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTV7"/>
    <dbReference type="OrthoDB" id="5411533at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12283">
        <property type="entry name" value="RRM1_RBM39_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12285">
        <property type="entry name" value="RRM3_RBM39_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029123">
        <property type="entry name" value="RBM39_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006509">
        <property type="entry name" value="RBM39_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48036">
        <property type="entry name" value="SPLICING FACTOR (PAD-1), PUTATIVE (AFU_ORTHOLOGUE AFUA_1G15810)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15519">
        <property type="entry name" value="RBM39linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="264"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="354"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="579"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="94"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2073778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="671" mass="76645" checksum="9EAF3D11F86FF92F" modified="2022-05-25" version="1">MSISKEEVASTSNNGNGATKTNAESLDVDALLEESFNKGLESSTNTTTLATMNEKKRSSPEDAESVNESESYEKKRKSSERDGSSSSSSSVSKSNDRDRSDRDRDRDRSDRDRDRDYRDKDRDRDRSDRDRDRDYRSSDRDRDRYSSRDYRDRDRDRDYRDRDYYSSSRDRYSSRDYRDRDRDRDHYSSRDRYRDDKYDKKSDYDRKDKYKDEKDTREGSSSRSSSNRDSKEKSTPPQTPKDKESAATTPATGIPEPDEESDLRTVFVSNLSPRITEKDLYDFFAQAGKVLNVSLITDRITKRLKGVGYVEFAERDQVQRAISLAGQKVLEQSIQVHAIQPEKKQPKPSSGGNARIYVGYIHIDATEEMIRTLVAPYGDVDFINIHTKPGVSKYAFIQFKSSENAKRAIQELNGKELMGKNLKLNMVSEEKNSNGTYIQVPQINNIIHHNYQPVPIGIPPPGAPNNMYHNNPMMHHMMMKQQQQQQQQYLMRAQGQGGSILGDPSQLGLANNPYGANNSGINNNQHSFRENNALSSLDEDDGGIPLTAQGRALLTAKLLAPQNTTTTTTTSTNNNTKTTCVLLKNMFDPETETADDWEQEIQSETYSECSTFGKVQHIFLDKNSKGCIYLRFDTNEAVQRAIAKLNGRWFGQKQILADPIPESIYKQKFPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU15</accession>
    <name>A0A8J4PU15_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ketosynthase family 3 (KS3) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_006003</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000258">
        <property type="protein sequence ID" value="KAF2072669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU15"/>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00833">
        <property type="entry name" value="PKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032821">
        <property type="entry name" value="PKS_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050444">
        <property type="entry name" value="Polyketide_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45681:SF6">
        <property type="entry name" value="POLYKETIDE SYNTHASE 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45681">
        <property type="entry name" value="POLYKETIDE SYNTHASE 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16197">
        <property type="entry name" value="KAsynt_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="4">
        <location>
            <begin position="8"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2072669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="631" mass="70288" checksum="DEFB6ED3F978A830" modified="2022-05-25" version="1" fragment="single">MNYQFRNRNKVAVIGIGLRLPGNSNSPQELWNNIKNKFDGVVEIPKDRLSENFYNIGYNGNNKGGFIDLNEWLSFDPLFFGIKPSDAETMDPQQRMLLKLTYEALEDAHINPIDIRGSNTSVFIGCYSLDYKLQLVNDGPLLQQESSYHALANRVSHVFDFRGASLTLDTACSSSINAFSLGFKSIECGDNDLSIVGGVGMIFNNDNYEYLTLTGAISPDGRCKSFDASANGFTRSEGCTLFVLKNLDKAIADGDTIYSVVESVNSNSDGNYEKENFNSPSPLAQVENIKSALSKGNIDPSDIFYFECHGTGTIAGDNMETKALSMVFKDNHSPNHPLYIGSIKSNIGHTEATSGACGIAKACLMLKNRSLAPNIHFNTPNPNIDFKNWNLSVVTECQPFPTDKKILIGINSFGITGSNGCVILSEFNSNSQDNNKNSNDNRNISNSNNLLIPLSSNSKESLNRYQSLIFDKEFIHQVGDKLDFNDFSKYHILSKPTNYTQRSVINSDSNWDSFSNSSTIISGEKEIISNMFNINNNPFVVFVYCGQGPQWNRMGADLYLKHKVFKDTIDSIDLELSKHFGYSIWEKLINTNDEESIHDPLLAQPSIFLFQCALTELYKQWNVNPNIITGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUJ7</accession>
    <name>A0A8J4PUJ7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_004852</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000180">
        <property type="protein sequence ID" value="KAF2073825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUJ7"/>
    <dbReference type="OrthoDB" id="419616at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17330">
        <property type="entry name" value="MFS_SLC46_TetA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF15">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="428"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="466"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="501"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="539"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="581"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="616"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2073825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="667" mass="73788" checksum="A2E8197A9047A99C" modified="2022-05-25" version="1">MAGDGVAGVPSPLPWKRLIGAYVLLVCEAISTTSFFAYSGYMIVYFGLVEKLEDVGYFAGYVASFFSFAQFLSSFFWGKMSDKYGRKPILIVGSMGSMLSVLVVGTSVNLPMLIAARCINGLLNGNIGVIKTYIGECTDKSNQVEAFGWIGLTWGLGSIIGPMIGGALIEPVDNFPFLFKNSKLFKSFPYLLPNLVIALTTLIGFTFTYFYMKETLHKKEPSIQLDDIEDQLTTGMTPEYIHDTEDIHGNGIIMKDYDDSEISGTIIGGNSNDIDGQDNNISNTKEEEKKNLLSNIIVGDEQSSSEYKFQDTNYTLNDDEKEKLYDPHHQREELENRKEMDSNIIALSESTTNFQDIPLDSTSLARGSMIKKSFSTIFNLFNSGKKEQYFQLSKDNSNSSPNLVGDGHGGIIVEKEQHKEVSVFKDKLILKTTILYAIVGFIFTMYDEGFPIWAMAPVSAGGLSFTARQIGATGAIGGVTVVIIQVLLIKPMTKKYGILKTFFIGGILSIISFFSFPIITYVSPYGGDSAHQPSPAKQAFFWFFMCIILTLRNIAGQFIFTPVMTLINNSARMKVRASANGLGQSLVALSRAVAPFVSNILAWSLTSGYPFPLNHFLMWIIMVALCLIPTIMSKFFLPITLNAPIVENDDDEIESEEESANTHFMIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUU7</accession>
    <name>A0A8J4PUU7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004304</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000149">
        <property type="protein sequence ID" value="KAF2074408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUU7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="93" mass="10886" checksum="5DE128B80EEEEF3A" modified="2022-05-25" version="1" fragment="single">MKRNTIHRNNNNSKKCKSNNKFRNNSSNNNSNNSRKNISNLPVHEQLFHKVWSNVVLRNEIFRIAGGDDSGLDCDEFSLKKKKKFRDYQRCGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVA3</accession>
    <name>A0A8J4PVA3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_008392</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000513">
        <property type="protein sequence ID" value="KAF2070286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVA3"/>
    <dbReference type="OrthoDB" id="529367at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF27">
        <property type="entry name" value="HLY-III RELATED FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="231"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="331"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="25"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2070286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="369" mass="42853" checksum="5DB81514AA18195D" modified="2022-05-25" version="1">MQRLNNNQQQQQQQQQQQHQHQHHHTLEKNVNDDDDNDKYHDDDSLSIDIPLSIEEGRGASIHNDCQCCEHSFETFIDLMLNENPFIKTGFRENTQNSFIECTKSCFQLHNDTLNIWSHLIAALFYIYLFFKSISIILNNNSNSNSDQIAAHDDILFNSADAPSNFNLLFFLFSCFLCFTASSIYHTYRSHSVPVFKKTLLFDVSSIGFLILSSVNLIIDSELSCFPRVKRIFLFSFLFLVFIALSLLPKIMREKKYGMRTLVFTLLALQGIVSHLLKVYMQGGESSSRDNITLRNLLVAYLFFGLGLSIRRLKIPESLWPGQFDIWFSSHQIFHVLVVIGTIFIYNTYHVNYLESNNTLDYLNKCTYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVE0</accession>
    <name>A0A8J4PVE0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004288</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000149">
        <property type="protein sequence ID" value="KAF2074392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVE0"/>
    <dbReference type="OrthoDB" id="21372at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050667">
        <property type="entry name" value="PPR-containing_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47939">
        <property type="entry name" value="MEMBRANE-ASSOCIATED SALT-INDUCIBLE PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47939:SF1">
        <property type="entry name" value="OS04G0684500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="500"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="507"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="807" mass="96553" checksum="19EA5936831FCD9F" modified="2022-05-25" version="1">MNLNLLKRLFSIHSSHSNSVSLFSHRLYTTSNSNNNNKKGINDQVKEKKEQLKQYVDHLKHEREMQDIQEFLGHGNREEDGDDDDDHYSKHISNKSWLPKKKYRFPQTNIRLYKKINSNSIKSFLLKDFKSYYLLNSHSSNSSNNKQRNIFDFGKGNTIVDKNFEKGQKEKEGKVRAEYLENKRNDYYRALQQHTRNQYKWYINKQARHLKQNRERVMLLANSKTVYNRVDPFENYFNVERLDKVIDDYIDNIEKEQEEKYIEKYKTLNEFDQYHYNYPSLTEEVNQDQDEDQDTDDGVDTIQLNYNIQYRDRVDLHLYSLFLYHHNNMYKEVFPMIYDNLQSLILDYQDKLLINESIEKEDFLEIVSMADETSESVFRRSNRVYNYFFKQLADKKKRIKFFQALESQEFNCELESQDYKKIVQALLEMRQFDSAEMVTQNMLDNRIFIDNDIFIAYLEYYRKDPELGQDNKQIFDAFVYQLKSLSTHYKDGLEYHKEKEFDKEVENQDQEEEDEDEEDNIAIRDQVLFEDILVYCYKNRLMEVGEQIEDFMEQEGIYPNMEIFNAKVKLKALEGEEISQRFLNDFSLVLGKKFKGNSHTFISMLHYELIKNKNPYAALSLLKEYKKKWSLKMNSFIYNQLIYGLARINDLPNALKYYSSWKKLGESKTVTEAYLQQKSAMMNESIILYCMAYYDHDPNSIIEFIKSNSIQITTPILDLIIYGYLIENKFRLEKYNSSNKKSTGSNSTDHLIVILDYLVFEQTKQPLDLSTIQFISSKLKSLKTSIKIQPTLKENLKKLKNLLTFKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXA8</accession>
    <name>A0A8J4PXA8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003161</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000096">
        <property type="protein sequence ID" value="KAF2075520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXA8"/>
    <dbReference type="OrthoDB" id="10394449at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="509" mass="57708" checksum="0DF7E46E0DCBFF95" modified="2022-05-25" version="1">MIDKTCLFYLVWRDSYIRYKIRVKVFENKVFQEKFLVKLIDNHQYIANISETDKLDNGIFICSSIRDTDNFIQYYNNQNKYAINDLKIEKDVIVQQLENSNSSNSRGGLIHDGVQRLTIFFTSSTCVGQGDQLPDSVTDLSLFRFGVGVNEFIDSILTNLPPKLRYLLVPDVVPSRCVLPDTMCNMYLSATPEVFNCFVVPPNKVFKGCKLRVNSPESLQWLHGRTWIITVLLHPFPFDSRIITKNQIPAHIVKLEILYTDPKIQVGALPASLQTLSVMGSLFVSIGTLPRHLKRLYLEDCPSKLEKGILPSSLRKLEIVRLNQSIDRGVLPDGLKTLVLPSLTLELEAGSLPNSLTWLSLDRYDVPLKSSILPSGLKVLIMSEYSGSFDQVVQLNSLEKLHVNKLDSSIARVISNVVKIKLRFYELGRRITLENTLIENLILSCQSYPSRLPLLPRFLPLSLRCLTVKGMRIQSSDVIPNGCVYLKSDIKDLQQDLLPKSVKSNLYKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXK4</accession>
    <name>A0A8J4PXK4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_004039</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000136">
        <property type="protein sequence ID" value="KAF2074644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXK4"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000003">
        <property type="entry name" value="Calmodulin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45942">
        <property type="entry name" value="PROTEIN PHOSPATASE 3 REGULATORY SUBUNIT B ALPHA ISOFORM TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="25"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="64"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="94"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2074644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="183" mass="21319" checksum="AC5023D655F5AE0B" modified="2022-05-25" version="1">MGVSRSTLRTEEIEEIQDVSVFSQREIKKLYKRFKRLDKEEKGSINVEDFNQIPELSMNPLLPRIISLFDSNRDGQVNFKQFAKTLSCFHPNADQHDKIKTLFKIYDINNDGFVTRDEIEKILKMMVGSNLTKEQISDIVDETLNEADIHNKGKLDYDDFFKSIEKIGCGQNMLSISFYSFDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXW3</accession>
    <name>A0A8J4PXW3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleosome assembly protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004566</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000164">
        <property type="protein sequence ID" value="KAF2074124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXW3"/>
    <dbReference type="OrthoDB" id="19419at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.90">
        <property type="entry name" value="Nucleosome assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037231">
        <property type="entry name" value="NAP-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143113">
        <property type="entry name" value="NAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="236" mass="27850" checksum="04D6F1D4A23F4ECA" modified="2022-05-25" version="1">MALGKKVQKVEQPKEEQVKTEKTLLEIIDENKDLKDTLDEIDSLIHQKDFSCMNVDRLLYDDLEPYYKERRDILKNIPDFWAVVITNFFLTFNTIDELTSYVSDVFIVEGKESIKLTFEFSKNDIIENDKIELVFQTPTCFEEMDKEMEITATEVKLLKKESDKKDKKDDDDDDDDEDFEKEEAFLEFLKKPTTDLFTSLVASIWREPTSLYKDPKSLVLDETDILEYEEDESDIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXY8</accession>
    <name>A0A8J4PXY8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Interferon-related developmental regulator N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_007160</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IFRD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000366">
        <property type="protein sequence ID" value="KAF2071516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXY8"/>
    <dbReference type="OrthoDB" id="18978at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039777">
        <property type="entry name" value="IFRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007701">
        <property type="entry name" value="Interferon-rel_develop_reg_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12354">
        <property type="entry name" value="INTERFERON-RELATED DEVELOPMENTAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12354:SF1">
        <property type="entry name" value="INTERFERON-RELATED DEVELOPMENTAL REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05004">
        <property type="entry name" value="IFRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Interferon-related developmental regulator N-terminal" evidence="3">
        <location>
            <begin position="36"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="264"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="277"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2071516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="465" mass="53342" checksum="491193D238173DEA" modified="2022-05-25" version="1">MNIRTRKSNHRSAVSLHKPLHENFKPLSYFVELLGNKKKSIVINALMNLDNILSHQDRTDEMFNQEPEVASDLMKALAALFTADKLDHYGKVSKVIGLLSIVNGPNHELYKIAKGPLSSIINDGENHVKENLLAHAIDALAFCCFICCTDIGSMEHTIESIQDLLLTQKRKLILDRALEGWTLLLTRLDNSKILHYRSVLDRLTDFTKSSSQSLRMSACVAIGFIVDEIKDNISLEGSTTDGNSHSAEDDYYEEDGDDFEYDSESYSHDEDLEEETNFSKDQEYGSASHRLENLHINEDNGIEGDGDLDSEEEEFEDEDEYEFNIDDDHMIQVIEETLQMNNEKGKKKIQSKSLLRQIISNLKDGEPPRETVIIRRYPFHFTGWRKYVQLQVMRRVLGSGMLYHWKNNTVLQDLFDVVLPENLHIKQMNAHHKKIMSPTSNESKKKTIKLNRNRDAAQYHLNAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PY50</accession>
    <name>A0A8J4PY50_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA replication complex GINS protein PSF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_007109</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the GINS complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the GINS1/PSF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000361">
        <property type="protein sequence ID" value="KAF2071576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PY50"/>
    <dbReference type="OrthoDB" id="10252587at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902983">
        <property type="term" value="P:DNA strand elongation involved in mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11710">
        <property type="entry name" value="GINS_A_psf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21696">
        <property type="entry name" value="GINS_B_Psf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005339">
        <property type="entry name" value="GINS_Psf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056783">
        <property type="entry name" value="PSF1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12914:SF2">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12914">
        <property type="entry name" value="PARTNER OF SLD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24997">
        <property type="entry name" value="PSF1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GINS subunit" evidence="6">
        <location>
            <begin position="53"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="DNA replication complex GINS protein PSF1 C-terminal" evidence="7">
        <location>
            <begin position="140"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU368085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2071576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="191" mass="22069" checksum="6C8249555CD5ACF0" modified="2022-05-25" version="1">MFTEKAIELLRELKRTDSLLAYGDTSIKRTVEEINALHKELIETISEHLEDKKTPYILSHSAVFQLSILRNKRCILAYLMERLNRIREYRWNSGTSLLSTDLKENLSPNESKFFADYDRLLTDYNAKIGLDLTMDPQPPKELFIEVRVLKELGQVVLNSGITVNLHLNTTHFLRRSDVSNLIAQGSLEHIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYJ6</accession>
    <name>A0A8J4PYJ6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">U1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_002152</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SF3A2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000057">
        <property type="protein sequence ID" value="KAF2076538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYJ6"/>
    <dbReference type="OrthoDB" id="10250970at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052092">
        <property type="entry name" value="SF3A2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031781">
        <property type="entry name" value="SF3A2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23205">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23205:SF0">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16835">
        <property type="entry name" value="SF3A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01050">
        <property type="entry name" value="CactinC_cactus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="U1-type" evidence="9">
        <location>
            <begin position="47"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2076538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="222" mass="25434" checksum="28E754D4AB73BE8E" modified="2022-05-25" version="1">MSEFGKAGSGGMQSSQYDGIDRRERLKKIALDSMDVSKDPYIISNHIGSFECRLCLTVHNNVGNYLAHTQGKKHQTNLSRRAAKEQRDNPTNKGFIQPSTTKIFKKTIKIGRPGYKIIKQRDKESGQLSLLFQIDYPEIDPGVQPRHRFMSAYEQKVETANKDYIYLLFAAEPYETIAFKVPNKEIDRSTGPDGKFFTHWDRSKNTFTLQFYYKEASATSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYM3</accession>
    <name>A0A8J4PYM3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">IPT/TIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_002134</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000056">
        <property type="protein sequence ID" value="KAF2076583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYM3"/>
    <dbReference type="OrthoDB" id="24390at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052014">
        <property type="entry name" value="Dictyostelium_Tiger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31341">
        <property type="entry name" value="IPT/TIG DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="689"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="4">
        <location>
            <begin position="142"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="4">
        <location>
            <begin position="317"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2076583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="728" mass="80838" checksum="F92A7554AE8E2F28" modified="2022-05-25" version="1">MNNISFLIPSMSTSLFNENITYSLDNKARISFKFPPGAGNIKLNPRNSCGDFGYQVPTIDSIGYSNNKIEIQGNNFFNEKKLINLSLQEPNMEPEIISADEMILVDHEKITFNRNIFSPQNLTFRLSIANRVAGSKTEIFRPIIAKINPGTIPTIGGKLTILGTYLNGDKNKPEIKIGDSICGSPIWASDGTNITCEMPKRSPNDKPFMPVIITINNIINNSTNNVSFEKPFITSLKQEKQWFNITGGNLGDPTIPTSYISFLFANGSEIDRLRKIPDKVTENQKNISTLLPSNACNGFLVVINGAQSNRVEVNIKPIITDVSMATTNGSIITVSGKYLNIFNCDEKKQNVTLESSDDKQFKCQFDTLDEKNKNGAHLYCQAPPGTGSNYSVKLTINNLPSDIFLFHYKAPIIHSTKQRGDSIVIIGEDFGEPLTLAYNRGKTARNFTTPAIVVVSNTELLFPIPQDLYSGYYNLIVANQETKGINQLKFGPIISSISRVPTSGGNVTIYGSLLDRISNFNITFVDNNPITDDIVCQTPFINGSKLITCVVQEGCGHSFTIVYKFDGDDSYDFNPDNITFSYEPPTILVYTLDENSILRVYGSNYNETMDISIKGNDERNKPLICTNVKLESVNQSSCYLPKFPHDFIAEPNENITIVVNVGGQVALEKIFIFDPSQREPPPADTKIKVIVPAIVIPCFLALVCGIVVSIILIQRHKKMKKLRKLFNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ24</accession>
    <name>A0A8J4PZ24_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ras guanine nucleotide exchange factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_002599</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000073">
        <property type="protein sequence ID" value="KAF2076085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ24"/>
    <dbReference type="OrthoDB" id="16782at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF151">
        <property type="entry name" value="RAS GUANINE NUCLEOTIDE EXCHANGE FACTOR S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="7">
        <location>
            <begin position="571"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="6">
        <location>
            <begin position="730"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="207"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="251"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="339"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="264"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2076085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="965" mass="108101" checksum="FC283912E913A9C4" modified="2022-05-25" version="1">MAEISLMGVTAIICQKDNSIWKNIDDSLCRLEILKSEKDSKFRLVAISTDNRCIFNKWVRTNSNISKCSDLFLEIKQVSSQSSTTDFIGVNFAYKEEVEKFYNCFSKCIETLQNQRELYIQQQKLGIANGADQLSRHSISISSSISFDGGLNLLANSGGIPPPSISTGTFSDLPLPPPPTNYYDNHTGLPIDLNSLPPPPPVVVITPSSSSPPKANTKVNTHHSVGFRLPPPAATTTTALTTAAANLNLGSSQTPINGSPPVEGSLTSSSSGSTAGNALQDLFAPPKPGVSITTTTIRVSANNTESTIMKTSSGRKSIRARSSTAADQQKKTIGKKDGLAISLQNVEGLQNIAENLEDETLNLLDLVNEKVVNPSLTNNNVFMQSIQKLYEHLQILFILSAESAKTHHGAKIVQQITSLLARGPSQNEYFSGISPTYQWYNLDEVNSSVAGIHNLQYVKKNLIPTIAHLSTAVRVLGLQASLEVDWMSRNKSSEPEKIVVQLAALSRELINAMVRLMAATTSYCSVCNSINTLKAESPSNINRIRSPSTADLVNIWDELKQLKTPLPTIPKDGSILKVTLNQLLLLLTSETNYDSKFMKTFITTYQSFASPGLLFSKLIERFNVPDWYSKDRQKILTIQQRVIVVLKYWIENQSSDFDQDVIDQIYYFIDHTLATSSDGYGGLAKQLRELLDLMIQDREVKFELLFQMPGRISFQEDSILSPIELFSEWSPQFIAQQLTLIDFSIFKEIEPRELLNQNFNKPKFKYRSPNVMRLINRSTQFSFWVAMTILMDGKKEKRLKNFEKFCDVAKHLYKMNNFNTLMSLIAGLNLTPVHRLKKTKKKLSSSATSMLDDCEKLFSSKKSFKNYRDHLASVTLPCIPYLGFNLTDLTFIEEGNPDNVSTDSSVPLINFKKRELIFQAWTDINHFQQTPYQFQVQEPLNTFLLHFPVLEEKELYDLSCALEPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ75</accession>
    <name>A0A8J4PZ75_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001801</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000045">
        <property type="protein sequence ID" value="KAF2076898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ75"/>
    <dbReference type="OrthoDB" id="20629at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="319" mass="36643" checksum="AC682C0FB53B8EF8" modified="2022-05-25" version="1">MITTETNNYVPTFWVNNDREVVDDVKTSDDDEIKQHQPPQYNNNNNTSIVDINNTNVSPLPNTTIIGNTITSSSSTPSSFTTSSHTLLFLKKYKSTFDLIDKQLLKWNNADQNILIVLRNILVSLERTLILLSSSSSSSSTTSSSSSSASEYKKNYFKVFEKSNDSTFNNNNVNNNTHLSITIENEIRLKNVLKLEELMSSLHSYQSKFKKICDKLKLYRSEMTIFLEKTILAQQPTATPTTSQQEYFKLLDLNSGSYNSPPITHVDDWLNYIVNTYNLEYQRRESITKQIQYHKPISELTKLVDHYENDSTINYSYSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZA8</accession>
    <name>A0A8J4PZA8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">FACT complex subunit SSRP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_006674</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the SSRP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000318">
        <property type="protein sequence ID" value="KAF2072011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZA8"/>
    <dbReference type="OrthoDB" id="498543at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035101">
        <property type="term" value="C:FACT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13230">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13231">
        <property type="entry name" value="PH2_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.150:FF:000001">
        <property type="entry name" value="Fact complex subunit ssrp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.220">
        <property type="entry name" value="Structure-specific recognition protein (SSRP1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013719">
        <property type="entry name" value="RTT106/SPT16-like_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050454">
        <property type="entry name" value="RTT106/SSRP1_HistChap/FACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048993">
        <property type="entry name" value="SSRP1-like_PH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000969">
        <property type="entry name" value="SSRP1/POB3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035417">
        <property type="entry name" value="SSRP1/POB3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024954">
        <property type="entry name" value="SSRP1_DD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038167">
        <property type="entry name" value="SSRP1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849:SF1">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21103">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17292">
        <property type="entry name" value="POB3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08512">
        <property type="entry name" value="Rttp106-like_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03531">
        <property type="entry name" value="SSrecog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00887">
        <property type="entry name" value="SSRCOGNITION"/>
    </dbReference>
    <dbReference type="SMART" id="SM01287">
        <property type="entry name" value="Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone chaperone RTT106/FACT complex subunit SPT16-like middle" evidence="11">
        <location>
            <begin position="372"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="181"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="471"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="181"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="481"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2072011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="523" mass="59056" checksum="4BDC7E102E1CE0EF" modified="2022-05-25" version="1">MSTATPAICQFNTITQGGKIGSTRGLLKFTNTNITWKSDGGRSDNVNISDITEAKWIRITPKIFQLKLSLKGGNSIKFDGFREQDSEVVRKYLKENIGIELENAELSTKGSNWGIVNVNGSNVQFMNDNKVGFEFSVSEVSQSVFNPNHKNELTLEFRHDKTLNDEDEAIVEIRFYTPTRPAKEIPDKKENEQGEEEEEEEEEEEPQEEEMSTLQIFQQSILEKSDIVSNVGKGLAVISNVHFLIPRGRLDIEIYSTFLKLHGKTHDHKVPFENISRLFKLEKSDQKSIFYVISLEPPLRQGKTKYSHLLIQIPNEEETTLELNLTDEQLIKYKGLTPTMSGSTVSVLSTILKVLSGKKTLSDSSFLGESGTASVKCSLKASEGSLYPLERAFFFIHKPPTYIKYEDITNVEFARLASVSVPGGLGNSRTFDLIVNLKNATSVQFTNILREEYRSLFNYLNERKIKIDAPTQASSGIQMAMDEDDSDDSDYEPSESDDDDDDDDDEEESEEEKKPKKKSKKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZD7</accession>
    <name>A0A8J4PZD7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome P450 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002917</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000086">
        <property type="protein sequence ID" value="KAF2075784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZD7"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20617">
        <property type="entry name" value="CYP1_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF417">
        <property type="entry name" value="CYTOCHROME P450 508B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="485" mass="56266" checksum="9927BE8D00C5E235" modified="2022-05-25" version="1">MILVIVILIFGYLVLDSIKKFITLNKISFKLKGPTPLPIVGNLIQIGDKGHKVLLKYHEKYGDVYRIWMGDYFTIVVRDIDMIKKMYIDNAYVFLNHSDTPSNQYFSHNFGGLPFNLDKSEWKKRRVITAGALSKVKLRNVYNSMNSIIGQLLENLKQHSISGEPFEPHYHSDKLLSDINFKLFFNSDPDRNDVKSKEFQKFSDAVNQIVVDLGTGKLSDFVYMLQPLLLKYYQWTYKHMNFIDKFIEREYNEHVETFKPEYNEDPRDFLDTLIVKMGEHYEGDDIKKMVLELLFNIFIASIDTTAGTIEWAYLYLANYPDIQEEVYQELKTVIGDRELLPSDRNSTPLLNAFIKELNRIKPVVPFNIPRLTTDDVEIDGIFIPKNTHIIANTYGVGYCEKYFPNPSEFNPSRFLNPHINYSVFSYGPRGCVGQHFAMDILFLSIGNIIKSYKVSKPNGTKISEEGKLGLSFRPFDFTVSIEERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZI2</accession>
    <name>A0A8J4PZI2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000040">
        <property type="protein sequence ID" value="KAF2077063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZI2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035310533" evidence="1">
        <location>
            <begin position="27"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="632" mass="72469" checksum="6330F4EF1CC7C122" modified="2022-05-25" version="1">MKRVTLYNYGCGLLFLLCLMVLRTESVSCQRDIHITGESNKMVDWWLIIKVRGFSDYYLYMDSLLQEDKFKIGYFLRSSKSALGSTMFPYTGKNIDEGKKSYINYNNQMVVFDQKSPFKTRNKDNLEYYKEGAHEKGFIAFNRGTNEDDWKGFFLQHSLPKMMNYREEGKLRYLNEPIDFSGRFKEKLNNLYFTYYGWEKPFFGRNLMPYSPQGIQLLQKIGNIENVRESQVDSVDLNTQEGQKKVADRENTFLTSGGTLGFLNMNPFSLIYTTLVKPSQHLLCISIENKASFEDLIGQLALTSNKGIVSTLYREHQGKTKAFHDFRKHLYLSEDSQNPTVYATRAEGFHKKDIKVKFETRETKFYTAVNVAGPKNKKDIWKNLIEDTNTNQPSLAAGHKMSISTWVTKQKSAWWRTDTYKTQVLEHKLQTKSEIDQSVFTWQSNSNQEHSKIGFTEKSDHPNWNFCSSGGNLYAKEKASMKSSLIICFTSNNLNKALNGLRGDTYIADVGANEDGEDTDDKIDGQTKDLIEMSQTKAKNLIIAVKGLTSSVTQEIIPQAPILTSVAKQLGTKAITFNDVRIKATRQNFNILSFSTTGFNEAEKNKSLWELIKNLIPPFVKAHFTLFLKSPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZI4</accession>
    <name>A0A8J4PZI4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003426</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000106">
        <property type="protein sequence ID" value="KAF2075297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZI4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2075297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="155" mass="18372" checksum="94EFF53A506FA924" modified="2022-05-25" version="1">MVYFKISFQSEDPFPLQAPYGFDSEFYFEFKIQFLILDINDIKSKNSCKYQTLYIRNDDEIRPKINESFIDGVRNINFQVSNLILFFYSNLSNWISSFSLISKKTKKFADTLLELRPLIESGNVGFYNICERSNIKSTLQKEENSNHMKRPFYGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZL3</accession>
    <name>A0A8J4PZL3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003356</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000104">
        <property type="protein sequence ID" value="KAF2075327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZL3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="77"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="104"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="171"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="105"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="146"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="171"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="219"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="380" mass="43709" checksum="B8384935E9E46073" modified="2022-05-25" version="1">MAKSSSKKKKSSSTHKGKEVKIKFVCRHTNGCQKPNRDSYARQRHEKTAHRDCDLSICPCPEHKEPQNPSAAVAKTFVCTFCSKTFGYRQALTDHQKNKSCIKDNSNGNSNTNGHHPTTTTTTTTTTTTTISTNNNNNIMNHNNNEEEEEDDEEYEPTKQSIDFNYYSQIQHNHQQQQQQQQQHHLHHQIQQQQQQQQQHHHHNQSHHNTSPLSLANGNHIHQQIHVQHQQHPQQQPQHHLHQQPQHHLQNHILNGIPNSNHNNNSNGISNHILNGIPNSNHNNTHHLLNNNNHNNHNNNNNNTNNNNNNNHLHLHHLQPLQSLHAIHSLHSPLPNLQPLPFQVSIKPEYDVHTLKEPTPFEVPILFDHATFSAKLPSPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0F4</accession>
    <name>A0A8J4Q0F4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002975</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000088">
        <property type="protein sequence ID" value="KAF2075732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0F4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="93"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="338" mass="39912" checksum="744A8BEA67F16CD0" modified="2022-05-25" version="1">MQGIKQIFKSNKKQKKMTKEKKDFEEQQNMDTDTTNKQTKKFRAEDLLQDKVPNVYDHTHYGGDSNENLKIAKLKEELQRDEDTTTHLREVKIAKLVSKKKALQEKLEKADKRIADLEKELQNKHLQNLLDLSKQVNTNIESSKESGERFISQTISELKTEIENLKQNIDERYSIQIFKSRENKLLLYIQELEKDKKGLDELKTELNQLKLQNQEQTNKFTQILSANNQLIQEQVKRNQQLEIEFKQTVKEKESSIAREQEANKLVVEMKSNKSLVDQVYALLEKQNVYQQIQIKEKKEKDELERIGNEMKVYIKAHFPSPDVTKERRNAFNDFIANN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0V8</accession>
    <name>A0A8J4Q0V8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CENP-V/GFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_001582</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfa family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000038">
        <property type="protein sequence ID" value="KAF2077135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0V8"/>
    <dbReference type="OrthoDB" id="2993351at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052355">
        <property type="entry name" value="CENP-V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006913">
        <property type="entry name" value="CENP-V/GFA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28620">
        <property type="entry name" value="CENTROMERE PROTEIN V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28620:SF5">
        <property type="entry name" value="GLUTATHIONE-DEPENDENT FORMALDEHYDE-ACTIVATING, GFA FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04828">
        <property type="entry name" value="GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51891">
        <property type="entry name" value="CENP_V_GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CENP-V/GFA" evidence="4">
        <location>
            <begin position="13"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2077135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="144" mass="16002" checksum="D1077C2D3DA13003" modified="2022-05-25" version="1">MEIEANVPKKDVVKGSCHCGSVKYQVEVDVKSITAYRCNCSICTKKRYTEFYAKPSEFTLIQGDDNLSSYTFGSHVTQHKFCKTCGVAPFLTGNIPELGGEFVGISINCIDDFTPEQMDSMPIHYFNGATNDWAGGPPKYSKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1A7</accession>
    <name>A0A8J4Q1A7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000599</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000011">
        <property type="protein sequence ID" value="KAF2078128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1A7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32142:SF55">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32142">
        <property type="entry name" value="B BOX-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2078128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="775" mass="91898" checksum="31E2C17B51DEA8F4" modified="2022-05-25" version="1">MNIHIYREVFHNIYLYKKIYDLVYEIQQHDNSLRYDDIVDVKWMIENKHFGLLREKIKANQLLYYCSGISFKLLFSLLDDSVELFISVFERYRHYLADCYTGGGNKNKNNKRDISLLQEIWKSNNIQVAKYFHQRGYLIHKLPFYVTLQDDTCPEVLEFFLNNGNIFLCHKLLSIFFSDVKKKLYHHDEIIRILQKHSTSLSMHQASARLRTLLLHPKPFLFDYYLPLFPKDLELSFDIEALLPKIKLNQYNLEILFWLYEKKFYCPSSSSKLNQFLKKRSLNGGDSMVEWNNYINQLENQQQFRFLNSTNFINSIKKDQHLLNSKNLFQLWYLDCFQNTQTKTTKNQIFIDTWNEFSDHIRVFPDYIYFSPTKPIPMDLDQEDVESLRDIKEISSHLLDIFCYFANIELISFIGDAGFDAQGIVDAIDIFSNEFMQNLSCYKTKQDFKLIDKLIQAEFIYPFYVLDSCTKYKNLPMFQFFLKKYPPTDPGELFLLFKRGVMDNCPDILNILKTQGFLMTRDVKIKIFKKGPFKNRLQFAKEILIDNSLNDKDTFLEVAIAQNDYPLSKYLLSNFDYPSMAPFKTKLSICYNIPLVDYILKYKNYQDSFCLSTVIQSNNLPFIEYIVKNNLTSIQDPLDNILIRAPILFLIKPFLDDSNHKFLLDSPIIDKKSTLQEIIYLIDQKAFKSIDYLLDFIYSQFGSIQIVEKDDLENLFFSLSIIGYIEKNLDQFENQTWFNQLNEKLQSIYTIIDLANPEIKYENIAAQGLDNQRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1E3</accession>
    <name>A0A8J4Q1E3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_005992</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000257">
        <property type="protein sequence ID" value="KAF2072686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="20118at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057709">
        <property type="entry name" value="DUF7949"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF29">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25820">
        <property type="entry name" value="DUF7949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1384"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="6">
        <location>
            <begin position="460"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="5">
        <location>
            <begin position="695"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="4">
        <location>
            <begin position="834"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7949" evidence="7">
        <location>
            <begin position="1095"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="3">
        <location>
            <begin position="1140"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1052"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1053"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1066"/>
            <end position="1076"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2072686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1426" mass="157515" checksum="BCAAC7D92AC4C908" modified="2022-05-25" version="1">MNYHNNLYYILFILYLAIGVFGAPTVFKNITNPNQNNYYPDSSSTSCTAYYEVIIQYNSTFNNFIKSETDNPLLSGFDKIYQSNGKALCALEYNIPLDTVSEITLTIQSGSLKETFSLGNYECTSSPSELTTSLVGSNNKMPYFWSLYFNEVVIKLDNLQKVLPVYVPPQDQDPIFSTNVSSFYTPYLAHYDFYENGYQKYKFFDNYFVIGFNIMGQMSTAGYADYISLVTSTLKAASNSILIRPFDTVTPTYTGSIFYPNAPSNTMRMVYGFTDVSSNKPHFIRFTRDGEPFWSAYNYPVYGNATKYTLFGFHTREIFSRSSFSFSLSFFSTSGTDIRPHSKNYNNYIVENGIDPTNPTFQSYNQKQSLFKTQMTYNFNIPSYIMNSSAILTMRKSIPLEFPFGFASGNLDSLTFSLAIPICQYFPSGSYQAIMQVPDSYYYNEYQNLVSAAGPSGTSTVDEEWPLLNSFEFVYNLGDDLALIRMSVSDVTSGVSTIILNTFRDGEQTYIGVGDLVSGTYKNGVYEKYVPFWYLSNIELITVLDNTLGYWWSKYYDGRIININGDVLHLTPGGEDVMPTPDSIVEFYFEKNPVDVTNKSVDNVLHIKVATVTSNTPYFVPILTFYYDPATNPVNFYGKWNSTTLGYDIPFTIPMNLVSSELIYDLFINPHILCNSGLAPIIGTNASLIVTSDNGDLLGPLISSIQALPATFPVTPSTNVAPFLVTIEDKLNGFSHGEIQITSNYDAIGYKFVFDESSKVSGDALLGSYIFNITLDPETCRSQIYVISQVTLYDKGGYVTSTSIPISYVNPLMKIGASPTLSTICSTVVDTTAPTVYSATLATATTLDVGSLDRTIKVILTSRDTTNTVSTDPRLAPYMYATTAGMIEIPKTQCVFDTIAENVYFKFNCTLTVPFGFGYPFGIYLSIYGIHDTQLNFGGLSSQEISIASIATTYTMNPLLLNASPLDSKQSTLTLYGKHFGTNKSSATLKIDYLNGTTASLPLVYPLFSGSIISSNSLTPTENPFSISITVGTKVSNALLIVPTVITLAPVESSSSSSSPSSSSSETITEVPTQKPTEAPTEKPTQPPVCPKDCSHNGVCTSHGCQCTPPFVGLDCSSRVVIIPEPSVNTTQPNSNTDYDTVLGDKKVRYSSLVSVVSLRELDSQGALVKEFVFEKWVFTNLSDSSYLYQYNITHPADGPIANTSVSVSLQWFSEATNITFANQIITMQPSSLKYKVDLSPYPFTSSFSTLQLVMSASLASSEKESACSSTSFNDDQQELQDQYVKIKVNDHSLYGRFIKRAVIDGGIKAITNTLLDADKNQVKDSSNQGSSAQSFIAINIPFYKNAIVIDPDFSILVETNAAQDDDNSVCTPKAKSGLTKVQMIGIIIGSVGMAVVAIVSVSYFIYKRRSFRKESIKLQTRFARD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q208</accession>
    <name>A0A8J4Q208_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002187</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000058">
        <property type="protein sequence ID" value="KAF2076509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q208"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035193127" description="FZ domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="246" mass="27416" checksum="D180DCC7AACFE897" modified="2022-05-25" version="1">MSFTFIYILLSLLLIIHISYGQNGGGDEMFVFNVTSKGDFRICPLSVGDLVRINATQMFQGPGENVGTYLIGQNIELDNKTVQVFHFENQPVDYAMDSYIEFVINQTKKVPGFSNQCIEQLVTYLCTYRYVKYGYEPCNTICDSFEKECPNTLMVDEAGTGLKFNVVPTVTSCLEHGVTEVNTTMCSSASVLGRFNFTDTMPLTKTNVIKYPGGQTKGTILGFGVLVGVLLIIFGLDLIMRFQQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2A1</accession>
    <name>A0A8J4Q2A1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RidA family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RutC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000053">
        <property type="protein sequence ID" value="KAF2076674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2A1"/>
    <dbReference type="OrthoDB" id="309640at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06150">
        <property type="entry name" value="YjgF_YER057c_UK114_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="entry name" value="RutC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019897">
        <property type="entry name" value="RidA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035709">
        <property type="entry name" value="YoaB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47328">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47328:SF1">
        <property type="entry name" value="RUTC FAMILY PROTEIN YOAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="YjgF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01094">
        <property type="entry name" value="UPF0076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="14131" checksum="16F9A43993105867" modified="2022-05-25" version="1">MNIVRIQPEARWSEAVIHNNTLYFTGVPDNLDADVYEQSVNVLQQIESMLVSQGSNKRNLIDVTIFLSKLEDFNEMNRAWDSWVIPGHCPVRCTVEARLMNPKFKVEMKFVAAVFIPSMENSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2N4</accession>
    <name>A0A8J4Q2N4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001068</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000024">
        <property type="protein sequence ID" value="KAF2077605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2N4"/>
    <dbReference type="OrthoDB" id="24277at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="588" mass="66591" checksum="0A1A082FBE6D5A61" modified="2022-05-25" version="1">MDTLFYALWRNKYLNDRVRLKRFENQKFTIDCSYLEENYRHLLTLYSAKNNNSSGDRDCNVSIELICSDADQFARFVRFEHRHLIDCLDLTIKGIDVNLFHSGALLHITKLTLHLDKFEKTRFDIPQQVTDLTIRGGDFEIINLPSGLKRLDADFYPVNLNTLPSGLTSLSVRQDILTLPNVFPPSLSTLYLVWGTIEGQPACQFPDSLTDLLMTFYIDELPTFNVPYTGKDFPNAALIIGDPAEVEHLELYPWVSVILFDEGFHNQRLDYKQIKSIVDYNGSQGLLIPGHLPPQLESFTSIFYHQSLTSGLFKNLKLLDLSDFDQPLAVGVLPNTLTNLQIPSFAQAIDQPDIIPNSVTKLSLKDILLVSPGALPSSITDLQLYSFTISPTTVNVIPNRVKTFGFGISPLNLTKGLSSPVQLPSSITDLNIYLQTLGSIKSLLRLPSALKSVTLSNVEIEKDLIPNGCYYLSTNYNKPISDDALPLSLKKLKLDLPYDHSEVVHVPPSVDVLIVSLKNLRFNRDVVVKELGVLRLMKKHIMEMKETDIDIRETILKKNLFLFTNFFCNMGRVFKNIIKKVCFFLPIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2N5</accession>
    <name>A0A8J4Q2N5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_001752</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000044">
        <property type="protein sequence ID" value="KAF2076914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2N5"/>
    <dbReference type="OrthoDB" id="16654at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14733">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051568">
        <property type="entry name" value="LZTR1/Attractin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46376">
        <property type="entry name" value="LEUCINE-ZIPPER-LIKE TRANSCRIPTIONAL REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46376:SF1">
        <property type="entry name" value="LEUCINE-ZIPPER-LIKE TRANSCRIPTIONAL REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="405"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="19"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2076914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="572" mass="65085" checksum="7AE069AFD664A9A7" modified="2022-05-25" version="1">MENIEVIGSNLPIDNEPSTTTTTTTTGVSLSPPLSPKTSSPSSSVIDNPKDEFSQQIDWKIIPTSNNPTPRFENTVVYHDKHLYTFGGSDGTTEALNFNPFLRLSLSDNSWQTLACPFKTRILHTAVTYGNAMYIYGGGIYEDERTGFFSSQKVFRTLGDMLKYDFEAKDWSEVAEGTNETRDGHCAIVIDNQMYIIGGSKTVTQNSKAISSVFFNTMLVFNFETLTWKTLSAPSFGRLCSATHYKDLSGNDCIYVFGGYGHGNKTNGDFWKYNVFEDKWNLVQKDGAPSPRYGQSANVHQDKLYMYGGYSSKGFHRDFYSFDFIKAEWTAIPTEGPPKRRRHSCLILTDEASGQARIVLYGGAYISCTYNDLWEYKFPSLVHIPSDKIVDDFSYLLEHAQEYFSDVTFLLVDDESKEPKEYKELHAHKNILAVRSEYFKSLFTSGLKESYEKVIKINERYDDFKVLLQFLYSGRADVHLGNCIGLIYLSDLYYVPRLKKICESKVTEGIDINNVIDIFKQADFYKLNQLRKVCLGYISRNYKELITKGSLNGLEASYLLEIMQYLDPTLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4A5</accession>
    <name>A0A8J4Q4A5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_000721</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000014">
        <property type="protein sequence ID" value="KAF2077997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4A5"/>
    <dbReference type="OrthoDB" id="17812at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042392">
        <property type="term" value="F:sphingosine-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF55">
        <property type="entry name" value="PHOSPHATIDIC ACID PHOSPHATASE TYPE 2_HALOPEROXIDASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="260"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="369"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="449"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="480"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="514"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="543"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="3">
        <location>
            <begin position="302"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="574" mass="65192" checksum="B8E1DB980236A438" modified="2022-05-25" version="1">MNTQTKHKNFCSSNNNIDIKDNHSSFLQFKLDQQNNSNSSRSSGRERSSSNSNIGDNYNNYYSNNNNNNNNDQLFDFTTDSIGFSNNSNSNSNSNNNNNIISRSSSLHKFEIEESIVGQYSVQDTLPAVPSPAGSTHSYSSTSSDNTLSLPTINSTLSGNGQTNNHYSKQSDPELASLMTPIEEYNEEYDDGTTIFAPPPINEEDHLLSNSRDFKSIYDANLSKRPKKRFVYLFPFWLEQKLKWFDDPIIEFCQRIHNRYIYYFSLFITACVAIEIAIAMPFILFILGQDSLATEFTYLGLLLALFSQIPKRFLWRFRPYMVKRAKLMKKDKTSSFPSRAVTCSVVYSYAIVWAVLYHGGGVENGWFRWWFPFLFIGMIGASSFARINLGVHYPSDCVGGVIQGVVVCLLGTGFRKADIVGCRSCWDGGCYAQIESRVLSLHTLDRLNFIMLCVLLALFIVIPIVSVMRPVDFWSKCDRVYGMLFPAVAFQLLMLCPRSYSLNGSLPKPSYPEWYSYLFGFSLAIITTLGASKIKIHSKYSILIFWTLFIVLSVSLFTWRLSLVGFAKVEDHQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4F8</accession>
    <name>A0A8J4Q4F8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_000573</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000011">
        <property type="protein sequence ID" value="KAF2078102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4F8"/>
    <dbReference type="OrthoDB" id="10259130at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000039">
        <property type="entry name" value="Cysteine proteinase 2, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0458">Lysosome</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035237943" evidence="9">
        <location>
            <begin position="28"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="11">
        <location>
            <begin position="45"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="10">
        <location>
            <begin position="130"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2078102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="353" mass="38829" checksum="D1188BD495971EDF" modified="2022-05-25" version="1">MYFTKVAIKSLMVVIFMVGMLANGMSAAAIPSTTKFTEMQYRSAFINWMNQKGRLYASEDFGTRYENFKANYDFIEEWNSKNSQTVLGLTQFADMTNDEYRANYLGTVFDASDLMPETEGLLHQTYIGDLPASVDWRQQGAVTPVKNQAQCGSCWSFSTTGSLEGAHQIRTGNLVSLSEQQLIECSWAYGNQGCNGGLMDSAFSYIIHNGGIMSEESYPYTSGAGDDNGNCKFDSSQVVATMSTFANVTRGDENNLAQVVVSGPVSIAIDAGHQSFQLYKSGIYYESQCSSYALDHGVLLVGYGTDASQGRHGDYWIVKNSWADSWGQQGYILMARNRQNHCGVATMASQPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4S2</accession>
    <name>A0A8J4Q4S2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FYVE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_004755</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000174">
        <property type="protein sequence ID" value="KAF2073931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4S2"/>
    <dbReference type="OrthoDB" id="20035at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052113">
        <property type="entry name" value="FYVE-type_Zinc_Finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39490">
        <property type="entry name" value="ARRESTIN DOMAIN-CONTAINING PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39490:SF13">
        <property type="entry name" value="FYVE-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="5">
        <location>
            <begin position="547"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2073931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="647" mass="74708" checksum="D1E7AE1AAD8A3EB4" modified="2022-05-25" version="1">MSIPSSPIYYNQTASFNIISPAQSSSSASTSSFSSPLSTSPIKRSPSFGSVIPPLPPSSSAKFPLELTSKNQTCIVRLDLDKTYFSNGSNITGKVIVNLTERQCLKRIKIQISGYEKIFQERENNTYQTLKFYTDDLNISPLNFIKDVNNPTITQHPNYKEQQFQQQQQKQQERDHPMSLSFNSLGFVHEFEAGTYEYRFCVKLPSYLQPSLNYIGFLSIFYMAHCKVEYSKGKDWKENKILKSTEFLISGIDLRYQDHLMAMKKSRTAQQLSSFKVSEFLWKDKSKPFEMALCLADKNLYIGDNALIYIKIKNPLETKFNSIKVDLFQQITFKKQQYPLLKLNRQHMAFFKHGNQFHPVASNSNLININDYTGNGNHNGEKSGNNPHKHKVSVTQILSLNFDSSHLCQQKIIDNSNNININNNNTNDEILSTQIKVTIPKSFEDDQVYPSTRGHLSTIKHYFIISIPSINNLKIKIPFTLWEKERYSPLTNGNSNNNIISPLSTSPKNDYLINNNLNIHNHLVINQEYKPPPENWKIFWLPTWKDETNESKCNLCDSSFSIIKRIHHCRNCGGVFCESCSSQKYSLFSYGIHKKVRICLMCVENIKEKQLHYFQLSQTKQERTDPPILFSKKKQLKYPFLIDIIDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q6I1</accession>
    <name>A0A8J4Q6I1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">tRNA (guanine(37)-N1)-methyltransferase</fullName>
            <ecNumber evidence="10">2.1.1.228</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">M1G-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA [GM37] methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA methyltransferase 5 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_003692</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>guanosine(37) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36899"/>
            <dbReference type="Rhea" id="RHEA-COMP:10145"/>
            <dbReference type="Rhea" id="RHEA-COMP:10147"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.228"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Mitochondrion matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="10">Predominantly in the mitochondria and in the nucleus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRM5 / TYW2 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.228" evidence="10"/>
    <dbReference type="EMBL" id="AJWJ01000119">
        <property type="protein sequence ID" value="KAF2074996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q6I1"/>
    <dbReference type="OrthoDB" id="408788at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052906">
        <property type="term" value="F:tRNA (guanine(37)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070901">
        <property type="term" value="P:mitochondrial tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.110:FF:000001">
        <property type="entry name" value="tRNA (guanine(37)-N1)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.110">
        <property type="entry name" value="Met-10+ protein-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03152">
        <property type="entry name" value="TRM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056744">
        <property type="entry name" value="TRM5/TYW2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025792">
        <property type="entry name" value="tRNA_Gua_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF36">
        <property type="entry name" value="TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25133">
        <property type="entry name" value="TYW2_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="12">
        <location>
            <begin position="113"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="405"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="407"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="437"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="241"/>
            <end position="242"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="270"/>
            <end position="271"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2074996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="446" mass="51399" checksum="F12A465116631499" modified="2022-05-25" version="1">MNKEIFKQSRQVLALIVSNRLIHNFSKEFKGHLIIRPCFKSIIPLESDDSKKIILLNEKISNDTEIPDNLQSFIKSNEIEVVKKTIDLDYKNFTYEQVLKTVLPENITIPHAFERIGHIAHLNLRDEQLPYKHLIGQAILDKNLQVKTVLNKVGKIDTVFRTFQIEVLAGEDNLVTEVKENECLFKFNFSEVYWNSRLQYEHFNLIDTFKSSDTICDMFAGVGPFACPAVKLKKCTFYANDLNPNSVKYMKENANNNKIDTTRLFMFNLDAREFVRDLLYPKEKSKKPVPFNHVIMNLPSTSIEFLDVFRDIFLDPNQNPPIPPPMIHCYTFTKNTEDGDLVESTRKNVEKVLNYKIPDDFTCFEVRDVSPNKRMMRISFVMPNILPYIGEEIVRVEVVDPKKNIKKSSPASIATTTTPSDSSTSKRTSSPTSNESETIKKAKHES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q7P9</accession>
    <name>A0A8J4Q7P9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002640</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000074">
        <property type="protein sequence ID" value="KAF2076076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q7P9"/>
    <dbReference type="OrthoDB" id="2012566at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43611">
        <property type="entry name" value="ALPHA-D-GLUCOSE 1-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43611:SF3">
        <property type="entry name" value="FLAVIN MONONUCLEOTIDE HYDROLASE 1, CHLOROPLATIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="223" mass="25884" checksum="D4CF5660AB153ACB" modified="2022-05-25" version="1">MFDALAIDLGGVLFSAGKEYAKKEWIEHGYDAELIHSLLTSPPSMDLRKGIIQDHEFWDIFFKSKVPESYDIDFIKQAFYRGYLLDDDIVALMRRLKGKVRLVAFSGNIPSRIEYLDKQFQFRKLFDSEVYSFDCGATKPDIYFIEYLIKHIYPAETANVVLHHGTPLTGESHQLFKSLGKKILYLDDSVKDAAPSKEYDINTFIYARGHIDKLYEKYPELKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q848</accession>
    <name>A0A8J4Q848_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serum amyloid A protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002254</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000060">
        <property type="protein sequence ID" value="KAF2076451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q848"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035261156" description="Serum amyloid A protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="124" mass="13843" checksum="F3A3850DBDA2D8B9" modified="2022-05-25" version="1">MKIISIVLLLLISTQVFAYKKMSQDRVLHDSQVAGGENLRSVDRWASEKFGGYRTGGTGVYANYIEPSLHAMEHTRRAVFNNNGEEWKRAKDEMRSVGNGGLNAMAQNQFSGGPNYQAGTYKRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4QAW0</accession>
    <name>A0A8J4QAW0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Polymerase/histidinol phosphatase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_000511</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000009">
        <property type="protein sequence ID" value="KAF2078221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4QAW0"/>
    <dbReference type="OrthoDB" id="16564at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052018">
        <property type="entry name" value="PHP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038032">
        <property type="entry name" value="CehA_McbA_metalo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42924">
        <property type="entry name" value="EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42924:SF3">
        <property type="entry name" value="POLYMERASE_HISTIDINOL PHOSPHATASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="18"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="318"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="365"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Polymerase/histidinol phosphatase N-terminal" evidence="3">
        <location>
            <begin position="86"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="435"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="445"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2078221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="482" mass="54897" checksum="C7234A8412E1B788" modified="2022-05-25" version="1">MKDTIHYCKKKIKDNVSIFYHVAFHFGFLVFVTLIVAVISVIIYNTPPRRYTGSNIEWPDASYDPRNLTIFDSDAFDGMDKINVFMDPHSHSSFSDGTLSPEQLIKWHILQGYNVMCMTDHNTIKGGLEAVKIAQEKYSEKIVVIPGIEWTNCRCHLGLIGITKNVPLIKFPTNQQIKEIIDQVHQQGGLVIMNHHPWSTWAGLDQPSYQEWMDMGVDFIEVVNGNTFDYQGYLFAKKNNIRFLTGTDYHFNGRASAWTVFSVNSSSPIGPRDSISAKGLTQEMVMDALRSKSTQPSFLFDTTGSNLMLDRTLKVNPLYTFLSPWIYLGGFFHSYFQIDRGMYSFVDGSCTKEHINIYSHQILSLIAWMVILFLIFEIIYWLIRLLIHLVRGKFFKKSRKSKHDDNGDDVVNADTLDNITPNSPMIIPYCKKTVGDDGSSNSNSNPPPPPPPPYFPPQYMMSNAPMAPPPPPPLYPAYNYPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPD9</accession>
    <name>A0A8J4UPD9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Kelch repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009589</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000751">
        <property type="protein sequence ID" value="KAF2069091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UPD9"/>
    <dbReference type="OrthoDB" id="14731at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF3">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="498" mass="56623" checksum="85DCED0072387576" modified="2022-05-25" version="1">MEFSNIQNFYIENPIDFNNNNNTNNNNNNNNLNSLNNNNIQLHNQQQQEEISTNLLELLDLIMGIQTDDTTTPNGWKMITTKRSNAILENNIQQAIPLNQSQENMMAPVYPNTASNLNTSQNTIEKMGSMPIERHGHTTCLYQNKIILFGGTPDGSSGLNDLYFLFLDTFTWAEVKTKGPIPMGRYRHSAVIIDDKMYIFGGYRSKCLNDLHVLNLETLTWSEPNCIGEAPSARSSHSVCNVGKKMILFGGSGARYSNELFSLDTITMKWTKHDVIGTPPSERWCHTMCGFGKKVITFGGSNDKRKDNKVYILDTETMEWSQPPTSGICPSPRQLHTAVTIGESMIVFGGWGKHQELDDLYILNTRTMKWICPKIETNFNPCCRQLHSAWVYNGKMYILGGYSKSKRMIDVYCFTPEQTVSSLRDLCIETIVENVNRYVPLFQYFPDEILYHIFSRLAQSGKSVPIQLDYLIKKSYLADHALITTKNTGPIEETLQYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQB0</accession>
    <name>A0A8J4UQB0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-acetyltransferase 9-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_008236</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acetyltransferase family. GNAT subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000491">
        <property type="protein sequence ID" value="KAF2070441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQB0"/>
    <dbReference type="OrthoDB" id="5043642at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000248">
        <property type="entry name" value="N-acetyltransferase 9-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039135">
        <property type="entry name" value="NAT9-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13256:SF16">
        <property type="entry name" value="ALPHA_BETA-TUBULIN-N-ACETYLTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13256">
        <property type="entry name" value="N-ACETYLTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="5">
        <location>
            <begin position="34"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00069551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2070441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="202" mass="23939" checksum="5D0EBA19B4070DB0" modified="2022-05-25" version="1">MKINSNTVIVGEKIILVPYKKLHVEKYYRWMGDESILEQTASERLTLEEEYENQQSWYEDEHKITFIILDKDLLPNREWKSNLDDEKSMVGDVNIFFNEYEEEGTGELEIMIAEKNSRRKGIAREAINLIMHYAMEKLCPKTFIVKIGEENQSSIELFKKLNFTQNGPANVFKEINMTCPINDQLVQSYKSMHQNLRYLNWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQE5</accession>
    <name>A0A8J4UQE5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">DNA replication complex GINS protein SLD5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_008124</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The GINS complex plays an essential role in the initiation of DNA replication.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the GINS4/SLD5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000475">
        <property type="protein sequence ID" value="KAF2070561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQE5"/>
    <dbReference type="OrthoDB" id="338231at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11711">
        <property type="entry name" value="GINS_A_Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21692">
        <property type="entry name" value="GINS_B_Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008591">
        <property type="entry name" value="GINS_Sld5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031633">
        <property type="entry name" value="SLD5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038749">
        <property type="entry name" value="Sld5_GINS_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21206:SF0">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN SLD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21206">
        <property type="entry name" value="SLD5 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16922">
        <property type="entry name" value="SLD5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007764">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160059">
        <property type="entry name" value="PriA/YqbF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GINS subunit" evidence="7">
        <location>
            <begin position="53"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="DNA replication complex GINS protein SLD5 C-terminal" evidence="8">
        <location>
            <begin position="148"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR007764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2070561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="200" mass="24064" checksum="89843D092CBF6068" modified="2022-05-25" version="1">MEDEFDLEERQLLYQLKTLWINEKFSPELLFYDQVLVDSILEKIQLKENICAENNDNIEHQFMANIYEMEIERLKYMVKMYLRARLQKIEKYYSFILQNDQIISRLSDNEKEYCQKFSEMTEDHLKQSFLNGLPKSFKNNDKENVNQPNTSTFVFCQSTDDLGDVQVEDDQIVDFKKGGIYFLRYEPIKFLVEQSKIKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UTE3</accession>
    <name>A0A8J4UTE3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Prespore-specific protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_004000</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000134">
        <property type="protein sequence ID" value="KAF2074686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UTE3"/>
    <dbReference type="OrthoDB" id="1112565at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08368">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00821">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12752:SF9">
        <property type="entry name" value="KRAMER, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12752">
        <property type="entry name" value="PHOSPHOINOSITOL 3-PHOSPHATE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="135"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="644"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="742"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="367"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="580"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="367"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="384"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="405"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="450"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="580"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2074686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="801" mass="90188" checksum="987F9422094BAC80" modified="2022-05-25" version="1">MEAQLNVLSTDGKSWKKRYCSLKNRILYVFETKLKSDENSSNYQLFYFVPLARCGNIATAVSNGPCGKNFCFKISYELDESTHYWLSAENEKEMNQWVHVLQEACAVKKEPERVMVKGRSKSVVFKPGAIQIAGISDHSGWLKKFSSTGGFRKTIQWKKRWIVLKDLVLYVYEGPESKLKTKVSIPNWKISIDESIGGFCFTLSHIGYEGITLQAETAEERAKWIQRIKENNRTLTTTLPNEHDEHRIKEWIKSLTTLTIDQLMEYLTNASFLVNIIGKASSQNIRVVLEATDTRKQDDEWNRKSFEAILSTLGMQGIKFVQDYSADDFLSANPIILKFALDLLNHFNPLFMNTPPVSAVATPSVSACSTMTSPSTLTPRSEASSISSSSSSSVTKEQEEIDATVDIKKSQEKEHSPIKQPEIKPPSPKTPIKNPEPTIKPVEPEPIKPTTESVSQPTSPIKPTESVSQPTTPIEPIEPVSQPTSPIKPIEPVSQPTSPIKPVSVVSPIKPAPVPVLKQEPSFEDQTTEEILESITKLSESQNFNLDEEIANLSSSINLNASINNNLNTSVNLNTSLNLRESMNNNNNDNNDNHNNNNNITPISTPTKISPRYVSPYRNLKRVETKDVIGEFDRIFSSLGKAYPKCHRCNEPITGNDIIELSGKSWHLDHFNCCACHKPLMGMDKIPFVEREGKIYCKDDHDKHFKESSCSQCSKTLLITFAHYGKLFCKEHYMSIPEIQANICQNCEEPIGNAPYQILETKKWHVDHFKCSYCKVKLHDAAACSVKNSLPYCKKCHISLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UUL9</accession>
    <name>A0A8J4UUL9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MACPF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010023</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000888">
        <property type="protein sequence ID" value="KAF2068653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UUL9"/>
    <dbReference type="OrthoDB" id="21110at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MACPF" evidence="2">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="580" mass="66445" checksum="68BC2EC0F0D0A491" modified="2022-05-25" version="1" fragment="single">HFYKKTYLGGKLTQISITESTNVRTENKDEWTESVSGSFSASVSSPTFSVSGSVSAAVDKSQSEQQQQDKQEESTISRMMVYGGIPAAFSPAEDGQSSPGFKEWASSIDVLPVPVDYQLYPIKMLLNSLWVNKHGVNIRALWEEAEISYYNGEAFFNEGPNPLVSYSLIYQFEISSPILNQTYWSSSLSPILDITYYVVENGAEATKNLKIPIEFQYTNCKGEMFVSDFVQDNTPAGRKEGVITAFYLNTPNPAGTQYRTFEPHPTKSLKYPLKFDFRTVDFFLSTKKPIIKITLPTGFPPLQYEPSKIISWKTYKAILFFSSSAGVISTPTNFYATAAFENYWSYNATSRYVPYGTMSGYPSSNTVPFTCTDEHHGSEQCIDGIEFQYRGPSVPISSSDPRPKPIYDPINSVFYPKDFRLSTPSRAWLSFSNPIPPTDYPKDLFYVENFRKIGINSRVNWLKIYFPNTNDPWRFDILTYHINNEKSIYDSANYINTYFADNTDLTYKFFKLESFGPTNLAEKQLYFYHNYNYNGGIIDSYEYFYQNAPYYSSIQGKNSNFDVFEDLEERTSHRFFKKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UV14</accession>
    <name>A0A8J4UV14_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Right handed beta helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_002548</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000071">
        <property type="protein sequence ID" value="KAF2076141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UV14"/>
    <dbReference type="OrthoDB" id="21116at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012332">
        <property type="entry name" value="Autotransporter_pectin_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31318">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31318:SF2">
        <property type="entry name" value="PECTIN LYASE-LIKE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035156652" description="Right handed beta helix domain-containing protein" evidence="3">
        <location>
            <begin position="23"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="531"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="Right handed beta helix" evidence="4">
        <location>
            <begin position="248"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="463"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="469"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="489"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="513"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2076141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="567" mass="62022" checksum="AE5605C0B721C38D" modified="2022-05-25" version="1">MWKTKSILFIVLVNLVCLLTASESITYYVDPSSVENKKDCGADITTACPSISFAVQSFLNNGSFDSSCLLTIELVAGDYYPSNTTYDESQPIPLFGLNVVIQPYKNQQVVISGENLIGDGPFFSYDDQNNGNRPTSSLLIKDIAFDQFKLPIINADVPSPLNIAFENVQITDSASMTAYPNQLFVLFSSTENQTTLSFTDCVFEGNANILLSNGGYVTFTTCQIKTNIGTSPFLSFSYYYEELPNTLVFNNCSIEKNSGSSHGIVYSMGGKVGFKNSLVKKNSAYATLVFYNANVDITGTSFYENTGTSNGAILVQNSTLNLNDCVFSQNSGKVGASIYSTSNLNITNTSFNENYANQGMAIYQTTNSGQDQRIVNIESSSFVSMYDSSTFSNQNMFYFSNAMVLLKDSSINIGTSRVSNGFVMIDCSSSSIKYVDTNVTSFNNYPPMSCSSCTVSNSNQESANIECPSSSSSTPNSNSTDDSSHSENHNSSQSNENHSGSQNNNNNDSNSNDNHHDSNENHHPSTKRSTFILESVLIPMGCVLVISSVFYIIIIHRRRNRGYQPIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UW69</accession>
    <name>A0A8J4UW69_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_001440</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000034">
        <property type="protein sequence ID" value="KAF2077251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UW69"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20617">
        <property type="entry name" value="CYP1_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000068">
        <property type="entry name" value="Probable cytochrome P450 508A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF417">
        <property type="entry name" value="CYTOCHROME P450 508B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0349">Heme</keyword>
    <keyword evidence="9 12" id="KW-0408">Iron</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="434"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2077251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="488" mass="56405" checksum="78BEE719C8F1BE91" modified="2022-05-25" version="1">MNILYYLFYIFLVYIFYKYFQKFKNYGPKSLKGPIPLPFFGNLLSLAPAPHVPLIKYHQQYGPVFRVWMGDTYTVVLNDVDIIKQIYVKNFQSSNAHPDTPSFHFYSDGYQGLVLSCEHDWKEKRDIVAKSLAKTKIKHLYSLLDNQVGNLIDAMGFHVESGLPFAPRKNIQRFIMNSMLRFIMDKEIPYDHQLVDGQEKSKMEGLLHHCDEVFADLGAGKLGDYIDILKPLLALYIKKSNKSLKKIIDIIHEEYIDHQNTFTEQHKTNPRDFLDVLIGEYLDDDKTNIQSIIFICFDIILAGTDTTSGTLEWCVLYLANYPEYQEMAYKELSSLIGKERKAQLSDRTSTPFLNAFIKESSRIKPVGAFGIPRMATQDIELQDYFIPKGAQILINYCGIARNPKYFKDAYEFNPHRFINSNQDFSMYGYGSRNCVGQLFANDHIYLALSNIILKYKISPTNGKKIDDSDVFGLTILPKEFNINLEERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UX46</accession>
    <name>A0A8J4UX46_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000486</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000009">
        <property type="protein sequence ID" value="KAF2078196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UX46"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2078196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="93" mass="10226" checksum="F3EED8AC45D1A345" modified="2022-05-25" version="1">MSAAQGPIRALPVGFIPNSVRKLALDKFSFTKDSIPTGCFGLYLSNCNYQAQDIPNNIGFLKTTITKSSTPIINEIQTIHPQITLKLNKVLTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXB6</accession>
    <name>A0A8J4UXB6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HEPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007977</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000456">
        <property type="protein sequence ID" value="KAF2070710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="19321at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058210">
        <property type="entry name" value="SACS/Nov_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052972">
        <property type="entry name" value="Sacsin_chaperone_reg"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047352">
        <property type="entry name" value="P_loop_sacsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15600">
        <property type="entry name" value="SACSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15600:SF42">
        <property type="entry name" value="SACSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25794">
        <property type="entry name" value="SACS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00748">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81593">
        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="2552"/>
            <end position="2655"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="2663" mass="307693" checksum="FEAD09BC1B8B6AF4" modified="2022-05-25" version="1">MKFTSGNAVEPYYKRISGILKKYHESTILKELLQNADDARATKVVIKLDNQSYNTDNLFAENDDFKKNMAEMQGPSLLIYNDAVFQEKDWVGIKSLGCGSKEMDLKSVGKFGLGINSVYHASDFLTIVSDQSLLIQDPLGKVSNNGLTMNFVEQPLESQNPNMTAPFKQFGCDMVNPFKGTIIRLPLRKQSSGLKKNPIQDYQPIFTEFIEQLKELLLFLQNISIVEVYFDNTLEFRVSITNHEEITRSGQRTLVQDYVSNIANSMDKYDIITFMNIIKKRNNLPIGTFIMELEYQYQNTKSSIRYCVSQGIVKNGLDKLTINDSNKLIPCGGVAVPLCTDEEMEGFQGIPFTFLPIGSLRYNVPFHFNGFFILSDARTDILFSSTTLEKSEEYLDSKWNENMLINIVPEIYNHNLNYLVTNNLVENIYTFFPVQEYVTEKDKLLSSAVLKRICQSSLFPDIKTKKYYTLQESKCVTSDHNQDICTVLHDRNITIYRLPSNLLKFIQDEGIPLTLVTKEYICVILNQYPLEQFNQSVLDYILSIDIIQRGLLNGLKVFPFVDNSINSIITKGNSFSFYYPDENQSTLLKPRAGKILFDRSKLEKYGTTIINQLSTYYNVLIMGSTDFLSLLKSSFPEIVGISTILSSLPSDFNQEAFWKCSVDKSSISDIVCVPATCRNIKYYVSPSYPELVLNSFGCTIEDIVVNLGYYKVDPSGKHESVVTSLDSYKNFVNGLNTYKVTCLGSSEKNKLRQFLLSSNNNQALSQRVFSLPIFTTSNDGFNSIDDYSTCTSWRDVSKYSNGKEFIIFEDNQSRLSTWPTKILSQIDVISTIILPNSNQYNTYDRISISQLILSDAAIFSEKSIIKQVCWVPNMNDSFSFVNDVFLLSHDKEKLLKYTSNGGNQIILKNLVENKEYFWQQLGVKTMFKNGDSDLIYDCLSNLPSLDLQTIKIAIPEVVDFMVRNQLVKDDFFIIPCVPVKKELSVGSITIPKISTVDFVSLKECYLDKYNDICCSVCNIADISPDCLASIEQYLPTPTINMVINHLQYFINLSKNTTPSPDQTEFSNSIENVVDKIYFQLDCSNDKIDELISKLEWVWVGSKFVSPKIVFQKGLTIDPFIFEIPDSLKKYTTLFSKTKIPMNPSFVHYSNILKHLYYTYSGQPLSLEDFEIAIQAMNNMSKLSPPQELPIIYLPSKDFILFDCKDLIFSQDDDLHPSLKQYSILHRKISLGISKKFKIKTSKDAIRKGKLVGKDFWQYKDIKYTIRNLIEEKYKVEYFLKEMVQNAEDSKATVLEILLDKRKYLIQEHDEDTQPNFKDYLSQSLVVYNDSIFSDEDIEKIQSLGDSNKRSSPLPIGIGFNSVYNFTNVPSLITRDSLYVFDPLENHFSVEESPGRSYEFNIRDQLKDEFQPLNYPNFDLDLDKKYEHTILRLPLRRSPTLLSKQTWDINSIQNIMKQYEKEAENCLLFQKYLKKITFSVIEDDKTNKSEIIFSVERTLKDNSFNSVQKDDSIQSYLNIFDMNNYYRPQPKSFSHYYFMDISKIENQHNKSSKLWFIGWKNGIEKNSILNSIKLEHPIVPFASIAMSVNDKVQGVPFTYINGLPLPIYTQLPVHINGSFILNSSPPDVFNNLPNDYKINLNDLSFMKGTENYKSVWNTEIVRELICPLYLEMMDHQDFKTLFLDRSDLASYFQLFPNSQTRLWSRVTEYFYQNVDKYNVFPLLDLEGEKLYFSWRSTGSLPQGMVSLSNNISLLNNSNIIKILVKDAGHIFMIPDWIYGKMVSKKNLMNPKNVSTFYKNLPVPFKLNRPPKPSLSLKENILDLCKYVTIDYLEGTPFMLLQDGQTCFRFQKNPEKLLYSSEFFSLLPLDDVFVHKDLCNLMFTSYPYSNLVLKLGFPDFFLNHLIPYLRKQTTYQGCLPILTSLKGSWEKFSSEHIQKLKKELFIPVLDNDNNVVNFNPASKYYSETVSSLINPKYHLPDIFYQSENILSNLNIRKSLKPSTIFKEFEALQYNPNETTFNNLYKYMETNLPRENFNEILDKIKFFKIIPCLLYGPTGSILEEFSSLSNSVDHSISILCFTILATRKTEFKLNFVGQNIYYEHFCNLLQNSSEWINNLVGLSSILKEVLSNLVNFDCSDTLPDTTLFPIGSQLVPIKDIICDPQNYYPFFSTVPSDFMYFRHQLEQIGFSKLSDASIVKKLQEMKGYRLDEKGIENLEALIKHLRKEYPPCLPDQTLCLRALTFIYREPETLDLLKRINIKGILYQVHKNFDDTYLFSLTDLVKEYLNEEKSKVKPIDNLIDFFDEKVLQTVSQQLRLINDQNNTLERLQTLKVYSGEIYSKFCFMDIEVTHQPKGSQYIYDPNSNSLYIQSQFCREINFCTYLTRIFSFNASILYKTSTEQEYDEYPFYLYSPGEMVIYRSNEESFENATIVEINNQCENDFMEMKLYFILTPSFEEPISVPQVHLYKIGSSTNYSLLQIEPQAVQTLYNETFGAFGDEFECKRIREFAKLSNIKLSPPKGGPKQFVPKFYTFRSEPKYSQTCTNPTMAASFIETSKMDLQAAQHCHNGGLYYHACLFAQQSVEKLFKSYCFKKGVPFELSRHTILYHLVEEDVDVSSAQLFTNYIPTRYPDGSSRPHYQYSFDDSKIALSTAQDLLKSMNPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXW9</accession>
    <name>A0A8J4UXW9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">UDP-N-acetylglucosamine 1-carboxyvinyltransferase</fullName>
            <ecNumber evidence="11">2.5.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Enoylpyruvate transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">UDP-N-acetylglucosamine enolpyruvyl transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_007048</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:18681"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:68483"/>
            <dbReference type="EC" id="2.5.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the EPSP synthase family. MurA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.7" evidence="11"/>
    <dbReference type="EMBL" id="AJWJ01000355">
        <property type="protein sequence ID" value="KAF2071640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXW9"/>
    <dbReference type="OrthoDB" id="7788545at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008760">
        <property type="term" value="F:UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019277">
        <property type="term" value="P:UDP-N-acetylgalactosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01555">
        <property type="entry name" value="UdpNAET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="entry name" value="Enolpyruvate transferase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00111">
        <property type="entry name" value="MurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050068">
        <property type="entry name" value="MurA_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005750">
        <property type="entry name" value="UDP_GlcNAc_COvinyl_MurA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01072">
        <property type="entry name" value="murA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006873">
        <property type="entry name" value="PRK09369.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43783">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE 1-CARBOXYVINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43783:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE 1-CARBOXYVINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0133">Cell shape</keyword>
    <keyword evidence="9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Enolpyruvate transferase" evidence="16">
        <location>
            <begin position="7"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2071640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="443" mass="48428" checksum="0A9270042F28255D" modified="2022-05-25" version="1">MEKLIIKGGKSLNGTVKVDGAKNSVLCLVAAALLGNDVYRFSNTPFLLDTFNTIKILQHLGVKIESDINDKTITIDSSGLDGWSIPFEYSCTLRASVLFMAPLLVRFGKVKIGYPGGCKIGKRPINYHVSALEAMGAVVTFSGDGVEDGIIEAHLTNDRFLAADVDLKSSVGASQQIIMAATMARGNTIIRNCAKDPEVVDMTNFLIKMGARISWRDETTLLIQGVEMLSSVGLLEHEIIPDRIETSTFMIAAAITRGNIFIENGVYHHNETTISHLKQMGVTIIQERKGIHVLGKSSSDTLSPLNVTTLAYPGFPTDIQQPMTIIQLMAQGKSVVKETLYENRFKHMDELKKMNAQFSVSSDGTELVIYGGNKLTGTQVVAQDLRGCSALVLAALISEGVTIVSNLEYLDRGYYQFHTKLCSLGADIQRINDSMHTIQCHHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYW9</accession>
    <name>A0A8J4UYW9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_004969</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000187">
        <property type="protein sequence ID" value="KAF2073710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYW9"/>
    <dbReference type="OrthoDB" id="28370at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 68F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808:SF9">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN GACX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="3">
        <location>
            <begin position="31"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2073710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="553" mass="62339" checksum="A3187471085235C5" modified="2022-05-25" version="1">MANKANRVSGYYGSNGSPVLSAASSTRLFGTSLIDTMTTQKRQYPDINIPLFLHHGFRYIIQYGLCIEGIFRIAGTKEKVKQLQTQLDRGETIDYLNSKVDPVDLADLMKIYFRELPDCLLQSDQYDHFINLLTLDRLGQIQKLRELVSNLRPENKEMLKELVWFLGKIAINANLNKMHEENLGLVWGPNLLWKGGKAYSTTDMMELMAGAGKIKMIVTLLVEEQDHIFNNINTPITNNSDMKISFIHKLSGYKKTLQGICIVENTENKSIWTADSGGVLKIFNSNTYAIEKEMDTNLGRIHSMTSIGEYAWIASSQSVSVWNKNGEMVKEFPGFHVSLAPVYSHGECRIWAGTEQKITIFSANNLEVVQTIDVPGQFLVSLTEIFGDSNQVWAGATNGVIYVFDKTSGTEIKQLRTPARRNITCLTFHRYNDAGKVWAGSEDKLIFVIDPASHEIVQTISHPDLLLINSLKSISNSVWSCSRDSSIRIWDSKSLSLIGSLDEFHTDAVTDAVLTWNSRKLRWELWSASFDKTVCIWSINSDFLPPPQPSPFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZE5</accession>
    <name>A0A8J4UZE5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_004242</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000146">
        <property type="protein sequence ID" value="KAF2074460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZE5"/>
    <dbReference type="OrthoDB" id="449052at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02205">
        <property type="entry name" value="CBS_pair_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF36">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="17"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="258"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2074460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="334" mass="37348" checksum="D81E44ED2FA2FFEB" modified="2022-05-25" version="1">MEDYYKIFHQTAISDIISKEETLIGINKNSSVEELLSVLFKYSLSSVPVVNDSTGKSRVIGFVDTNDILTLLVKLYEKVNEQGVNEDDIRLLSIAFLHTSISNIMDISKKDQFTVVLEEQSLLEVLKLYSKGIHRVALLSVFSDIETIVSQSNVINFISNNVSLLSGVKDLKISQLLPMLVSRESLITTKDSDLTICSFKSMKTNHVSAVPVLDSNKSIVGTLSINDLYGLKERKVNQALLKPTLEFIQNKDTDPNKNKPLNPVVLKLDDTFKDAIELLSKYKVHRVWVVDEHNTPLSVISLTDICNIIVNPPLETQELEIDSSQQNRASIPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZW8</accession>
    <name>A0A8J4UZW8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_005075</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000193">
        <property type="protein sequence ID" value="KAF2073618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZW8"/>
    <dbReference type="OrthoDB" id="6079689at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01310">
        <property type="entry name" value="TatD_DNAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000005">
        <property type="entry name" value="TatD family hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018228">
        <property type="entry name" value="DNase_TatD-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46363">
        <property type="entry name" value="DEOXYRIBONUCLEASE TATDN2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46363:SF1">
        <property type="entry name" value="DEOXYRIBONUCLEASE TATDN2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005902">
        <property type="entry name" value="DNase_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01137">
        <property type="entry name" value="TATD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR005902-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2073618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="332" mass="37793" checksum="FC7373582CB16589" modified="2022-05-25" version="1">MSSNNDKPTTPTTTTSQPKLKEGCCDVDNKPSQKQKQQQQPKKQQQQQPKKQESSKPKLNTPPTVDMPNDIKYVDSHVHVDQILIRLNKTLDEFPIFQKENYPIQFEKCVQVCCDPVALEYTDMLVQFDPIYAAYGVHPHNASEYTDDIEQKLITRMSHPKTVAWGEMGLDYYYNKSPVDIQKQAFSRQLVAAVQCKKPIVIHSRDAEQDTITILKDLVPKEWKIHIHCFTSSNLDFAKSLLEYFPNLYIGFTGCISFGNSQPIRDAVSLVPIERMLLETDGPYMTPMPFRGQVAHSGHIPLVANVISQVKNIPLVDVLKQCRINTTNVYGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0U2</accession>
    <name>A0A8J4V0U2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AAA ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_009023</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000619">
        <property type="protein sequence ID" value="KAF2069662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0U2"/>
    <dbReference type="OrthoDB" id="27435at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19503">
        <property type="entry name" value="RecA-like_CDC48_NLV2_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19511">
        <property type="entry name" value="RecA-like_CDC48_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000567">
        <property type="entry name" value="ATPase, AAA family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003455">
        <property type="entry name" value="Nuclear vcp-like-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000300">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase TER94-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF27">
        <property type="entry name" value="ATPASE FAMILY GENE 2 PROTEIN HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="6">
        <location>
            <begin position="7"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="302"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="570"/>
            <end position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2069662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="800" mass="88954" checksum="5B07FE10800F6EEC" modified="2022-05-25" version="1">MQEPSIKLTVSELNRNESTTANTRKLNVSYLSVRAMQQNDITIGDIIYIVNSNSNSSNGNSGSCCVSVAWPQQKLQSGHILLDSIQRENCNVRIGDTVLLYKTPFQVINSDSFNKRIMNMYPHLDATRLQVICETPSSIAYVDQLNDLGMLSPLIISQITDRYFIKSNSFTININHLPIKFKITNIINSSTSSNSNSNSSSNIDNKKEEDISLLFNNLNISSTSSPNVLPIFRVNLNTDIEYTSSREKETKDTKDTKVLGSSDSNLNFNLIGGLKQQVEQVKELIELSFFKGDLLKSFGVKAPRGILFYGPPGTGKTLLARIVASQTKATLFTINGADILDKYYGMTEKTLQNIFQQASKQAPSIIFIDEMDALCPKRDESSTEVEKRVVGSLLTLMDGTESSEKVVVIGCTNRPDSLDPALRRPGRFDNEIEIPIPNVSGREEILKIFLSKIPNQLTEEQVTAIASKTHGYVGADLEALVKESTLKCFHRLDITNQSSALLDNVSIDFDDMLGALAICKPSSMREVVVEIPKVHWNDIGGQDDIKQKLREAIEWPLKYPQSFERMGIKPPKGILLYGPPGCSKTLMAKALATESGLNFIAIKGPELLSKWVGESERAVRDIFKKARQNSPSILFFDEIDGLAISRSDEGSGAVERVVSQLLTEMDGINPLSNVTIVGATNRPDIIDKAILRPGRIDRILYISPPDFESRKEIFNIHLKKVPHNDIDINHLSNITDGHSGAEIISICREASICAMKEDVNATMVKMSHFEEAIKNSKKGITQEMLDFYKRYQDQSNLQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V159</accession>
    <name>A0A8J4V159_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008541</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000535">
        <property type="protein sequence ID" value="KAF2070142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V159"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="647" mass="75826" checksum="E7EFD334970E88A5" modified="2022-05-25" version="1">MSSHDKITFLNVYRHSFLRYKIFSFLGLGRKDLNLFKNQTIYKSYQDLDVKDLVRLGNGRLLREKLCKFKSLLQDNKATGAKIQYQDWLDFVGGKQNFSKDLFKNQQEIDEFLNICRELGFVTYRFFKDFINFYGFTPERADLLDLGTEKWEFHSSIQKELFLYLLDKKKLQVYNYQQVVVAPYFNECRDLFRTIPNWSDADLLYPVLSSNDHIETFKWLAQVSDLLNYDLYYQKYPNKLPNNQDNQVLQQLYGIRIQTDHPVLVEYPFGSIEIFHFVKNNHPIQLLKYLNNPFYSFAIKDLDLAKEFVLYLKENDIVPTMLLDFQVIDIQLFKDLASLGVGFICPLDSFSTMAFVLESNYQNWSSFSNFYKFSDYKALDSEIHGVLDKIQENPQVMSVCKQTFYKALNKRDLKFVKLFLTMINPAYKVTIKNVTSAYESGSRRLYEYVLAHWTPPQEKTFDDLYIELLKVAIFVKANVKDVCLFSKSIVNNDLVNQLMSFTYKSLSSASGKKKEIMIKIVEILKPRFLNPVSKLDKNLYYQDWNFLGLDFTGAMEALRTSLVNGCLATFKIVLSHMTSDEKEKLSNQIESIVLNYFKTNGPLYYKVPLYIFETFNFTLESFKELYALGQELGNQTLMDIVSSIAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V196</accession>
    <name>A0A8J4V196_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008422</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000517">
        <property type="protein sequence ID" value="KAF2070262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V196"/>
    <dbReference type="OrthoDB" id="23204at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23524:SF1">
        <property type="entry name" value="MRH DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23524">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G04850)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035327937" evidence="2">
        <location>
            <begin position="21"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1201"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1210"/>
            <end position="1220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1244" mass="139299" checksum="51EA815960F37FF9" modified="2022-05-25" version="1">MKYLFVFLLLLYVSFIKIDACQSPILTSNSFILQCSESSFGLKGSFAVLHSYKNKGYSFSFSPEVKDFNIGYKGTYYSLTPGLNYTVTWQHETDPTCNNVTYVYVPQSTFEISQPKCRYSQGSVKFIPDNNMNSPYISASCQDAPCNFTRFQDASYLYSDTSRNLYCFYGTPYYELSDKYPNLKVQDSYYYNSSGSIQLFDLSKYTFVNLTKNGNSSFLIPDSPVNSGNWINLDGRESYNLYLESSDCGVQNIPIYISQRWPDYYFEFGKNESCPRNFTIKMVNEDPNIYEQVEVIVDGQRVIQKDQLVFRADPTSNSIETNFVLIGDSNVGANLNTYAPNILGDVHYSVDPYTRLVTLYYNDTLINDLYAMNGEYNIEIVNKTFNLPEGSVFIESPCHFAKIYITLPPPTLPSFTITKPQEYCGDTFDVYVANYMDFEQLTIGPEEYEHDGYGYFRNINPSEGFINYLSNGYDEVLSVNFELTQYKPGEYEEIIDIISYPACSKSGNVSYTLRDIKRNILSQTTYFTFYPLGSIYLITSFHGCNQDIGTTWISPNIENNKNNFPKEFYPIVLKNTTCKYSNDGSLLFPTNPTIQINPIKVNGITYQGTSTLAGFVFENLPYGTNDYILESSMCQFVEAIFIGTDNDWELPVTVTPVTGDCTVANGGISYNSSIFSDFSVANAQNINQLASDYYHLDFTLTNGTCFGSTKVYVTSNVQQYYPSGKILTQPSCDSVMDGVVQLFVNDKNGKSFTPTRSTYNLQSDIFSVYTVQSGQSDFVIYKDVCSWKLGLSIVSRDPEFIYSHISDNLESCSQQTIYSIVPKDKNITINDVTSDQSLEKFSNNLFAYPSTSFGLSKHFMIRYNSYCLKTIIVDAVKDLRINIPWPTINIPSTDCTDPSVQNKVAQISNPSNMTLCANEETYSLKNLYVPTYHTDSAYFFAQDRKTSCFKNVYYNSANIGKVPNTVTKPTCPGGDDGGIQPSFNPDSLYQLFDNEYNILPIGSNSNTYKNISNTQYNLIRSFLNNPFCSVVENIKVEVDEPTVSLSSVGVCDASNTAIGDVGVVTNTLSITTTNVTYNLNGHVSTNPVFKGLSAGDYSSTVTIYNSVCKRTIKSNSVSVAKLPSVSVNVDVSTCMKAVINPSNTNIQFKYTIRDSTDKVVYEITSTGSFTYTPISIDTYSVTASDMSKTCSKKTSFTVTECPTQPPTNAPTGSSSSTEPMPSSSFKLIPSIISLISFAVVFYFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1F6</accession>
    <name>A0A8J4V1F6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_003262</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CCNDBP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000100">
        <property type="protein sequence ID" value="KAF2075433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1F6"/>
    <dbReference type="OrthoDB" id="41588at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1410.10">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.10">
        <property type="entry name" value="Talin, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026907">
        <property type="entry name" value="GCIP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049317">
        <property type="entry name" value="GCIP-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049318">
        <property type="entry name" value="GCIP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15492">
        <property type="entry name" value="CYCLIN D1-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15492:SF1">
        <property type="entry name" value="CYCLIN-D1-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20936">
        <property type="entry name" value="GCIP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13324">
        <property type="entry name" value="GCIP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-D1-binding protein 1-like N-terminal" evidence="9">
        <location>
            <begin position="41"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-D1-binding protein 1-like C-terminal" evidence="10">
        <location>
            <begin position="233"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="193"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="193"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="210"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2075433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="364" mass="41875" checksum="2587642040A50489" modified="2022-05-25" version="1">MTSTVEKNYEGIIGKINTFIDTLFSKNTDTETFQRPTREQIIKSAKTLSHEITKICLLLQDLRAKPEFFDSIEETLDNIFSVYQGLAAYSSDSLFKSNQVHFRRLMKSVLDIFVAYKRNEESLGEDEENIIDKQLTARAWKSCEDIEKITIKNSIAIIERNKEISSMIQDASEEILEFQEKLEKIKASFGQVKQDKQEDFKDEQEKQEQEDKDDDVNGEFEQDDEEAVYDLDDDLEVIENISQQEVAMVDRVAATIEKSLSLSKVFGDIIKSNEKSKDKDDVSQDTKENVETMEKIINLLNELSKQVDDIASAVYPPQNLTYLESQTSDMDVEIFDIITLIKSLPFYNSISKDLQSNLNNLIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1I3</accession>
    <name>A0A8J4V1I3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_003185</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000097">
        <property type="protein sequence ID" value="KAF2075493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1I3"/>
    <dbReference type="OrthoDB" id="448448at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF22">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="843"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="1171"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="28"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="201"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="289"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1222"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="58"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="218"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1222"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2075493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1270" mass="144405" checksum="C4F118E88D40E716" modified="2022-05-25" version="1" fragment="single">MLFKATEGSLPIPSSLSTDVEMVDVDVVNNSNNNNNNGSNITPKIESPPLKRTRSTRGVRTTPSYNEQLDLTRLGQSTTATTPTAPAPAPAPTPTSPTHSIESITKPTRKGRTTKKEAKVKVETQQEDDSITNIVLVQTPLKDTTSIKTELIAPQEPPPTVTQINASNLKSSTEILPPVDVKAEPKKRGRKKKIKIEVANENNSNTPPNTAKPIDLSTTDTKPILPTTTTTTTNTATAIAKKIVNNSVVVIEEDNKNEKSIHLIDDEPKNNSFTTIIDDDDEEVQEVPINKHRKNNNSILDLEDSPKLTSATGKSTTTTTTTTTTTSTPKPKRTKIIKVLVKPELTKKQKLEENHRLDGYEWSIELLKVLGVSNQDLPYIKMMSIKRVSNSFSQSIRNALDFLKAQNKSDKVTQDMSFLDEPDKTETMPYYVDQEVTDDEDYNEPSITKKKTTTTTTTTTTATALKKSQSAPNQMYVDLDDDDDEFTDTPSFRLSQSSSKIVPTIKKLQLDPEDLALVGTKSTGDKRNSDDVDIDEEYEKTKDFDIEEFNEKRLIDFDSPLKKEAQPERVEIDLQQEDINEDKNWFVGYKKKLFKNGQAVAPDENFFIHVTKIPDLTLVVANHAVLVIKDKEPLYFLKSQPGDPHKADSILAFSQKTHLFLGYLIPPPNLTASLLPMFRNKFIRMKGKAILKGYMSNTIQAFATIDIFITHRAKISLFDRTSPSADDMSDTTQRILYLHQFLLNVLGSGITFGTNDAGDKKDTLTEKDFLVKKTLLKEDTPPTFESTLQTHQKEGLWWMLQRERNPFVTYKFSTENYWRIYTTKKGVVFYYNMYADKISLEPPKTKNKIRGGLLCDEMGLGKTVMSIALIMSNHPIFSPGPHQEVAFQDIRPNLVNRYQRNKAKKRIQNPKTTLIICNSPMVSQWRSEFDKHLKNSGDLKILVYHGSNRRKECAFLDFAAFDVVITSHQIIGLEYKHYVEELSRNPYTPIPTLHTIHWWRVIIDESQVCKLKTLIFKALQNIDSVFKWCLSGTPVQNYLEEIFPMLHFLHVYPIAQTIKIWRNLIDKPKNITLLKKCLNPILLRRLKLDVKDIKLPPKQIEMVTLHFNPIEASHYRILFDHSQERFQDLCRRGAVFKNYATVLLLLLRLRQCCDDKRLIRKTEDKKEEIVCNYCDDISANTMKNLCGHTFCMECVDSILKDAATDERDAVCPQCDRQLVVTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNLTPVKKKKKGGEDDEDIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V337</accession>
    <name>A0A8J4V337_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mps1 binder-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009308</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000676">
        <property type="protein sequence ID" value="KAF2069374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V337"/>
    <dbReference type="OrthoDB" id="184876at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.30">
        <property type="entry name" value="MOB kinase activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005301">
        <property type="entry name" value="MOB_kinase_act_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036703">
        <property type="entry name" value="MOB_kinase_act_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22599">
        <property type="entry name" value="MPS ONE BINDER KINASE ACTIVATOR-LIKE MOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03637">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01388">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101152">
        <property type="entry name" value="Mob1/phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="25938" checksum="4F6CF2A131BAA36A" modified="2022-05-25" version="1">MASEEQQPQQQPQQQPQPTKRRNRIGTKNKDLYKWEPTTYELNESSYATQEYIQDRIRMDETNIDAILRAPEGHDVNVWLYEQIRQFTLELNHFSVLFKDICNQNTCPKMKATDDWLFLCASHPAIQECSAIDYFIHTLDSTSATLNSEKLFPKRMEIPPSSAKQFNSIVRRLYRLFAHAFYHHRELFNDFEAKTQLCKRFTQFGKDNGLLPKSAIIIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V349</accession>
    <name>A0A8J4V349_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002191</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000058">
        <property type="protein sequence ID" value="KAF2076513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V349"/>
    <dbReference type="OrthoDB" id="18809at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF57">
        <property type="entry name" value="METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01209">
        <property type="entry name" value="Ubie_methyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="285" mass="32390" checksum="B4DBE612ECB121E6" modified="2022-05-25" version="1">MSNSVKQTKIDQTAEGWGNCNRVRATFSKNVYTAQYANDCMDLTMGTRDIRDPNIKILDVAAGTGALSFVALDRIQNSNNSFILATDFSEKMIAYMNNVIKQEKYKNIEAKVMDGQCMSEIQDNLFNYVYSVFGAIYFPDRAKGFREFYRVLKANGKMAVTAWRVDTSFVKIFTYAFKQLVGDESLIPLKNTVLSLSDKHVFKSELEQAGFRDVEIHSVTHYMSFPTVQAFLAHWQQNPVYEDIIAFLTPARKQEFDNHILAYLAANYPDTTKIPCPCYIGVGTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V367</accession>
    <name>A0A8J4V367_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_006394</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000293">
        <property type="protein sequence ID" value="KAF2072287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V367"/>
    <dbReference type="OrthoDB" id="20872at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00173">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="F-box" evidence="6">
        <location>
            <begin position="79"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="5">
        <location>
            <begin position="166"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="321"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="367"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2072287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="626" mass="72060" checksum="056F57A2D6B3820B" modified="2022-05-25" version="1">MEIHPSIMTDHNSSINSNSRVGVTTTAAAVSKGITAINNQLSNNTNQLAALYLQNYRQHYLPYHHHYHQEDQDTSNEYILTINDLSAEVLINIFKYLSPFDLRIIAPVCKYWSFLSEENCLWQEKCISQWSWMFDVLNEKVKYKANSSWKEFYKFWAVEYLKHSEWYQYSLSRDQANSALKYLGKGTYLVRSSGSKPNSLVISYNVHSKDPHHLLISNLGSYIGVFLNDEIGTIYPTLASLIKDKKRFLKRPMKQSKLFESKSTTKRNYILKMISDPKSRNGKSLNQLKLLHIACKWCFKDLVQNLLSIAGVDINFRESKKGKTPLHFAVGFVHGEPSSNQRTEIVQYLLMSGSDDQKGDCNVLDFKGRSPLHIAVKQHQPDSPDLVEILLSCGADPSIYNHKGLYPLHISVKENNLRTVKSLLKHERVDVNCKIESTKYSKLDNSFLDTPLHISSQNSFHPIIKLLLQCKDININERDYMNRTCLHRAVLNTQDWLSITRFGKPLRSYFAHQTLELLLKSNADPSLLDNENKTALHISASKGHRKSTFILVRMIKGLKLSDLDIFKKEDLLDSIEIGKHSKDVEKIINDLKYSMFCGFDQTELESVQKTINDHYKNISDIIHQHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3I1</accession>
    <name>A0A8J4V3I1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PIH1 domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_008869</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PIH1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000591">
        <property type="protein sequence ID" value="KAF2069809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3I1"/>
    <dbReference type="OrthoDB" id="546764at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050734">
        <property type="entry name" value="PIH1/Kintoun_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012981">
        <property type="entry name" value="PIH1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041442">
        <property type="entry name" value="PIH1D1/2/3_CS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22997">
        <property type="entry name" value="PIH1 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22997:SF0">
        <property type="entry name" value="PIH1 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08190">
        <property type="entry name" value="PIH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18201">
        <property type="entry name" value="PIH1_CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIH1 N-terminal" evidence="4">
        <location>
            <begin position="89"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="PIH1D1/2/3 CS-like" evidence="5">
        <location>
            <begin position="256"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="50"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="345"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="51"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="348"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="361"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF18201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2069809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="470" mass="53984" checksum="F030B53AB7A47360" modified="2022-05-25" version="1">MSQETNTTTKQQQYLSEKDYKQLTKAFQDPKIFNSLLEYYDQVKKANQDDNNANIENSYSNNSSNSNHDKSSSSGDTTNTTNTTSNSQEIKVKTKKVNTVFGFCFKTHSNKRDKVFVCVSHYPTVEPASVVDGKGWIIPYSLFPLERQQSSFTSPYHSVYHIVYSTETYNESVKVPTLHRLLLQTAVQAIGERYAESLNIAVVKMTKKMFGKELIVQVLDQPTDINLEEPPPKEFTNPDDNTIQPVHYIEHLDKHKKKLDNMVIIPSFIAIDILLPKLNNIDDLEIDIEQDDNDQDEQQYLVVSDLISKYNLRIKLKHKVDDEPLAKYNKTKKILSLTLVVKEDHSHSHNHSHSHSHTHTHGHDHTEAETDLHSNNNNNNNQDTIEKFKNLNIQSDPLVFSSNNQLLVPSLSFPILQENICINSTNILLKQTLQSILIILYINTNEIDKESTTIVNNNNNNVRYHHHYNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V455</accession>
    <name>A0A8J4V455_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">26S proteasome non-ATPase regulatory subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008234</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000491">
        <property type="protein sequence ID" value="KAF2070439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V455"/>
    <dbReference type="OrthoDB" id="10250600at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019538">
        <property type="entry name" value="PSMD5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13554:SF10">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13554">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10508">
        <property type="entry name" value="Proteasom_PSMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="512" mass="57975" checksum="45D4E039CC657C07" modified="2022-05-25" version="1">MENKLIEQFNKLQNAKNEDEIEDICFEINSLIGTKINAPRLFLLPEMLPYLQLGLDINTHPSVISLTLKNIGVLAKLSTEETCSFLLHNKLVDPIFKLITLDSISVSSSSSSLLAKILSVRNKGTDKFIEAIRKPFIELLVQQQQGDGNIVFRLLDLGSSVLIHQYRNESMINLDTDSYILEQSNIDELESHFTADAVKFKEIYLKTLEDSINKGDELIEMNAIEIVQNICSKAYGIKLMDKEGIFSKLYQQLNDSVLKPKDTQTLHRSLVFNKIIQVICSIASSGGDLACLFIFKNNDWMAIFQYHLVNSGELDISKRLLEKIISSIGPLGALNNGIQFLINHQPLIQNYAELILSNDTTISIATMISFSLMLDNLDKNSSNSDKYLQIYNYIPNSIFISKIVKNLTNPIDEVKSSTLMLVNSLCMHSWGAADMIKVPGLLEYLLNRLNENTKLFREWKFTIISTLVKYQQPLFQSTAEERNRFLELKAYIAKGIHYIPSEAKVEIATQDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V497</accession>
    <name>A0A8J4V497_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UbiA prenyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_001356</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000031">
        <property type="protein sequence ID" value="KAF2077353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V497"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042371">
        <property type="term" value="P:vitamin K biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13962">
        <property type="entry name" value="PT_UbiA_UBIAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1780">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026046">
        <property type="entry name" value="UBIAD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929">
        <property type="entry name" value="1,4-DIHYDROXY-2-NAPHTHOATE OCTAPRENYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929:SF0">
        <property type="entry name" value="UBIA PRENYLTRANSFERASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2077353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="25344" checksum="D0C4D39081829965" modified="2022-05-25" version="1">MPFTTLSVGVIFGYKELGVISPNLLHVFVLIQLIYTFSVILNSLYDSEVGVDIEETSKDRVMFDYGLTREDIFIYIYGAMFLIFLLFVKIFFYFPMWKKMLALYLVFHLLFVSICYNMPPIRFKKRLLMAELSIFLSYGVLTFTGYFFSTGVVAIHSFIYGIPCFLLVIMSITSNLLIDIEDDRRGGSRSTPMVFGVKASLYIYIFSYILYVIVNNNIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V569</accession>
    <name>A0A8J4V569_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_000878</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000018">
        <property type="protein sequence ID" value="KAF2077833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V569"/>
    <dbReference type="OrthoDB" id="192247at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016235">
        <property type="term" value="C:aggresome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005164">
        <property type="term" value="F:tumor necrosis factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070842">
        <property type="term" value="P:aggresome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19756">
        <property type="entry name" value="Bbox2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16619">
        <property type="entry name" value="mRING-HC-C4C4_TRIM37_C-VIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053003">
        <property type="entry name" value="TRIM_RBCC_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36754">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36754:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="19"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="5">
        <location>
            <begin position="114"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="439"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="539"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="601"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="862"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="989"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1078"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1152"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="442"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="543"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="601"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="640"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="681"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="868"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="879"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="919"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="945"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="989"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1008"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1079"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1157"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1191"/>
            <end position="1208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2077833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1208" mass="138473" checksum="16CDF222D9956013" modified="2022-05-25" version="1">MTGSNKTSLQETIQDIIKCSICLSNTPQIPRFCPKCSALYCLECIERWIDTNPTCPACRDNLSHETLNSNLIVSNILSELTKNQQLQLQSSQSSHTTVTTTTTTTTTLIDNTQQPQDLCLYHGYERLYYCTNCQEPICSDCALFRHMDHKIEKLSVNFNKNLEILKIEEEALKKRQEELIELNSFLESDAGILFKNKAQIEKEVSKITSAIKYRLREDFESRYSIILKSQEKINDELNHLDNVYSDLLNHYRKSPSSFVLSSSEFITTLQDVHKNSIEDIYKPINTDLNSELVPPFEHRLLRFENFLSLNGDLFSSRISFYGMTWEIKCGIEDDDCFLNLKVLECNSEGKYLYMVTIINQKSNVNKLIEFDLPFQAGETFYIPNFSSKQNLIDEGFINPENDVLLLQFSFRPQNYYYYSLDLKHYINMLESRSLNRNDDDINSGNNNNSNSNNNGGVVETTITKYSDRSNLDFDKSFFNDLDINDINDININNNNINAILPKDSLSSILTRNNNNINNNSSNNINNRLTLINVSKLLDHDSDTDSENDDDDTDSDTDSDSEEIIEDFDFNTNHLMPPSMTDNQESFEDDSDINLYDDIDDIAQDQDDDDDDDDDDDDFGQDDDEQDNRNIIGHHALTESNQEDEEEDDNQDEDDETNDDDDDNDQDDDDEDDDDDDEDGFSESSNVSSIESSSLVSPFDTDEDLNSMLMLSSSLGNRKNIKRYSLPIQSTTNNNIINSNNNNNNNNCNNGSNSKQYPYSIRNRRVFNDLMKRINKRNDEIDNVNHNIKNSIEQINLCNPLLDGYIEKRNRSQETRRNSFHGNSSSSIVDIIKSNNSHSSNNSKPKEAITVDQLENKLFNVSLVNNNNGDDDDDDDDQDEQKQEQEQKQKQLLDPLFLQSHENTENEDFYQETTTETENNNDHDGGESEFDSNKSYEGSTKNSDENYSDFDENDYEDENDYDQDKHLQELNHSFYNPTIDVYYKNQNNNISSNVNNNNNNSINNIINDNNDDDDDDDNMGDSDSDPDEENDDDNDDNTGDTITDDDTTDYDEPLIYQNFDFPPNSITTQASVLVTTTIDSSSSSSSSSFSSSSSSSSSTYSSESEVDDNVDNNNNDNDTLYQDEDEIINIDPKNLQKISPILRAMAAQDLERIKKLEEQEEQEQEQEAQLEETNDNDNDNNDQQDQEYNCQTKTNTNTTIKTNSNSSFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5N6</accession>
    <name>A0A8J4V5N6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DUF221 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_003886</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000128">
        <property type="protein sequence ID" value="KAF2074822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5N6"/>
    <dbReference type="OrthoDB" id="16947at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF5">
        <property type="entry name" value="RE44586P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="420"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="460"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="507"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="551"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="579"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="612"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="639"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="9">
        <location>
            <begin position="11"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="10">
        <location>
            <begin position="193"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="8">
        <location>
            <begin position="363"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2074822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="732" mass="82540" checksum="918E53E226C2470B" modified="2022-05-25" version="1">MSNRYQVQDSAFVVTLVINTVISILSLLIFCFIRTKFKKFYQYRYETNQRGVEISPRTGFFAWIRDTLNYDNEKIIQTSGLDAYMFLRNVKTNILICGTLMVLGAIMLYPTNAVGKYNENRPNDDEGNPVDPIVGLATISMGNIERESNLLWVHTVFTFIVTAVVCFFSYGDYSEYAKRRVQFKKENRLLNHSILIKDINESSFKLDAMKQVMNSYLPNNGLKDVALYYPSPIALKLVSQREVFVKKLERALFDQKKPGSKPPMVKTGFLGCVGEKRPAIEFYSEKIADLTKQIETERQASEQQMPAARAAFVVFNQKQSAKQFVQTIMDPNYPCEFGTHYAPDPNDVFWSNVSIGCKSFFIRSLIVGVFIFFLTFFWAVPVTFLSGFSNLGTLAKVSAFSWLVDIINKSSVLAGFLQGFLPNLVLIIFMALLVPIITAVSKAQGFHSNSEIDESVFSKYFLFLVFNVFLVSAIAGSIFQSIESIAKDPSSIVGQIANSLGGLSFQMINFVMIAAAASLGKIGRFIPLLIRQLKLRFFSKTKREREATKKCGAFGYSTSYAFNILVLQLCLAYCTLSPFIAIFGVWFFGATYLVSKYNIIWVNTPSYQGGGILYPLSFRRTLVGLLIYHILMIGSFNLYGFYFGNLTVIPLVFTILYWVYCETQFHSKSQNGILDKYENAEEQPILEDGGVGSFADTTVAFTEYNGLQYKSPVYQPLMEPDESNGEQGNSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5P9</accession>
    <name>A0A8J4V5P9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003816</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000124">
        <property type="protein sequence ID" value="KAF2074897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5P9"/>
    <dbReference type="OrthoDB" id="10687594at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="148"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="271"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="768"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="358"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="467"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="148"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="181"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="768"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="783"/>
            <end position="795"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="828" mass="95029" checksum="A7C5602AE623CC0E" modified="2022-05-25" version="1">MSFRQPQQQQQQQQQQQSSDSNNTSQQQKLAKSLFGFGNSNNSKRSSTNSLPSTPTTPTHHNDNDIDAILLGGNGNKKTTTTTTTTSTSSTSNNTNNRFNSKSIPLSTSDLLTNKVSSFSSKNPSSSSSTPLTPVNSLAQDMDILKNGRLNSLGSNSRGSSDTVVRVTTPFPPKNHHQHQHQYQQQQQQQHEFLEIGSESQTYESQQQLQQQDSPIGYFDEDFEEQRSHRGQKKAKKSTTQKEFPFELYEDSQIYMETEPVNTTPLINTFEQQHKDRQSSPPTSVLKSKGYKLSNSPTISYEPMMDNSTNSNNNNNTNNNNNSHNIFEMNDKTNLYFQQVYKSIASIEEKSQKSHSLTESLFGKIKSLKEQLANANNTIQYVSNENNNLLLKYSELDSTFIKNQFETASIKTQFQLGNEELRVTCSKIEYFDQFTEKSESQSSELSLVKDMIEKTLERFQLERVDLLSQLDQKIETINSIARELKDVKNAYEKEVKDLQDKLLKYKIDNSGLESDISSLFKSKTNLENEVANRVSEIEHLNIKLSENQNLINDLNHQLETMKLNCLEKDSQMETFKSENIEIKSNLAELEYKSKDIEERLDQIVLENKSLITKYQDLEKEKETIDKDLLKQTEQNNSFFQHISKLESQLSQQSTNEKEWSDNKSNLEKEIQMLNETNTRLFDEKQSLDNSNSQLLLQISDMNKKNSKLEMELKNIINDSTTKHKTLQERYDKLELDYKQVKSNFSKSLLQPNLSTEEPNMLTEMNQMDQMNQQQQQQETNQNVTDSSFDTQTSPNKKPAATVRIDPPIGLITRKRKSSFVGALDENKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V620</accession>
    <name>A0A8J4V620_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF202 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_006329</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000287">
        <property type="protein sequence ID" value="KAF2072359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V620"/>
    <dbReference type="OrthoDB" id="199599at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052053">
        <property type="entry name" value="IM_YidH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187:SF2">
        <property type="entry name" value="DUF202 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187">
        <property type="entry name" value="FGR18P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="309"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="DUF202" evidence="8">
        <location>
            <begin position="236"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="40"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="107"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2072359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="332" mass="37289" checksum="3ED9FF8D3521ABFC" modified="2022-05-25" version="1">MENDGEKKSHHDDNDIGNQDTNQSIPHNNSTSGSSNSAINNNNNNNNNNNNNNNNNNNNNNNNTDNNTDNNTDNQYLDDINNNNNNNNNIRNNDIKHSIDNIDVNGNNNNNNLKSSNRNNKKRSSLKSSTNSLRQRAYRNTIRKKSSSTPSLKEKKKRVSGSDGEDDDDNKNNNNDEKEKINDKDKQDDKDNSNNDDDNEQEIDKIEDEKQRKLLKRYYRKILFSKILENKGSVARDHLANERTFLAWLRTSLGSIGLGVALAKLGTSQAGGIIFVALGSLILLYCPFRYFQVYRELLKGGFTPNIKGTIVFVLLTLACAIAALVIVVTKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6A9</accession>
    <name>A0A8J4V6A9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Restriction endonuclease type IV Mrr domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_006046</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000261">
        <property type="protein sequence ID" value="KAF2072644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6A9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007560">
        <property type="entry name" value="Restrct_endonuc_IV_Mrr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04471">
        <property type="entry name" value="Mrr_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Restriction endonuclease type IV Mrr" evidence="3">
        <location>
            <begin position="198"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="4"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="100"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2072644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="311" mass="34762" checksum="B4CD28BD2DCDB7EB" modified="2022-05-25" version="1">MSVLKQIEDLIELQKDTLKKIEEKENLITKIKDLNSRVEKRINDLRRSNDALKDRLESLSRTADGQSVEAWGQHSDEDDGEFTDRAEQDYYDSRRAADEIDVIEQEIAWNEDKIDELKGNIGSYKNNIQRLRSMISTYGKDIENHKLRLGKISDEITNILNAPGSPSTNESFGQPSGKILEIISYHYLSTIISPGESLEIVGGKGDNGVDLKIISSDKMVAVVQCKQGNLEGKGPAIVRELIGTCIIHGVKKAYLVLTSDTITTSTRETINLAETKGIQITEILAPTLKIHFSKLNLESFKDLVQLFTSAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6C4</accession>
    <name>A0A8J4V6C4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_005995</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the BMP lipoprotein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000257">
        <property type="protein sequence ID" value="KAF2072689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6C4"/>
    <dbReference type="OrthoDB" id="17301at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15047">
        <property type="entry name" value="7tmC_GABA-B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051530">
        <property type="entry name" value="mGluR/GABA-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003760">
        <property type="entry name" value="PnrA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46924:SF3">
        <property type="entry name" value="METABOTROPIC GLUTAMATE RECEPTOR-LIKE PROTEIN C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46924">
        <property type="entry name" value="METABOTROPIC GLUTAMATE RECEPTOR-LIKE PROTEIN C-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02608">
        <property type="entry name" value="Bmp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01176">
        <property type="entry name" value="GABABRECEPTR"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="352"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="419"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="459"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="516"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="547"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="577"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="14">
        <location>
            <begin position="360"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="616"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="616"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="641"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="684"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2072689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="704" mass="77503" checksum="1546B2386EBFEDC5" modified="2022-05-25" version="1">MATILSGDPSDMGFNYMVNQGRIETEKYLGIVESKAFENVPEDGAAPIVKQLVQEEYDFVVCSSLGFINDCKEAASTTNRTVFLIRGSSNPTLSSVYITYNYASCNYISGYFAGLVTKTNMVGFITAGPPTINISNANAFFVGARKSNPKVTMLFYTVGEWINSDVTRAATNDLLDRGCDIIGTTQDDMSAGEEVLARENTITLGTNGYPQRKVFGEGIQFSYVYNWTKHYSAAAEMVFNKTFASQVPAKLKFYGDFDTGFITLDYGVFVSDEIKVKVENERNYLASIPRAQHPYICNEYNKYVNIPNGSITGDCMTTAYFFRLDQPYPGMEYLGKYEIQLVKVDFGKNIQYGFSIVGGCCIFICLLAVIGILKYKDTPSIRSASPIFLIFIICGGIIVYGGIILWVSGNTTQTCNARFWLVSLGFSTLIGSLVVKNFRIWLIFDNPELKTVKITNFQLFPWVGVVVAINILLMAVLTGYGGLRSTDAQNIDNIGKYEYMSICTMDGPGHATLYTLLGYFAALLLVGVFVSWKIRIVDIEEFNESKAIAKTLYAISFCLFIIIPLMTSPQDQKNQTIILAAAGLFIVCSALAILFVPKFWRIKVYGHGGSNEMFTQKKSSNIASSRAESASKNSEGNSSGQHKHTTHTTRRGLQNDFTDDSESSAADVPPPKPSLPSGPVLAEFTDDSASDTDNDHDDPIGDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V991</accession>
    <name>A0A8J4V991_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">D-aminoacyl-tRNA deacylase</fullName>
            <ecNumber evidence="2 5">3.1.1.96</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_002840</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a D-aminoacyl-tRNA + H2O = a tRNA + a D-alpha-amino acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13953"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:10124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59871"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:79333"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>glycyl-tRNA(Ala) + H2O = tRNA(Ala) + glycine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53744"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:13640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the DTD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.96" evidence="2 5"/>
    <dbReference type="EMBL" id="AJWJ01000083">
        <property type="protein sequence ID" value="KAF2075854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V991"/>
    <dbReference type="OrthoDB" id="275783at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051500">
        <property type="term" value="F:D-tyrosyl-tRNA(Tyr) deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.80.10:FF:000001">
        <property type="entry name" value="D-aminoacyl-tRNA deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.80.10">
        <property type="entry name" value="D-tyrosyl-tRNA(Tyr) deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003732">
        <property type="entry name" value="Daa-tRNA_deacyls_DTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023509">
        <property type="entry name" value="DTD-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00256">
        <property type="entry name" value="D-aminoacyl-tRNA deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10472:SF5">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10472">
        <property type="entry name" value="D-TYROSYL-TRNA TYR DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02580">
        <property type="entry name" value="Tyr_Deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69500">
        <property type="entry name" value="DTD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0820">tRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2075854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="152" mass="17420" checksum="555168718739D8CC" modified="2022-05-25" version="1">MKAILQRVKSGSVRVDGETISKIGPGLVCLIGIGRDDTKKDMEWMAKKIQNIRLWKNENGDKNWDQNVVQKGYEILFVSQFTLMLTLKGNKPDFHLSMEPEKAKAFYHEFLEMSKKLYSSDKIQDGRFGAMMDVELVNDGPVTIQFDTQKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V9I9</accession>
    <name>A0A8J4V9I9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Myosin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_002561</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000071">
        <property type="protein sequence ID" value="KAF2076154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V9I9"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031252">
        <property type="term" value="C:cell leading edge"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070685">
        <property type="term" value="C:macropinocytic cup"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043327">
        <property type="term" value="P:chemotaxis to cAMP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000007">
        <property type="entry name" value="Myosin IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF743">
        <property type="entry name" value="MYOSIN-K HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 7" id="KW-0518">Myosin</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="9">
        <location>
            <begin position="7"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="127"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="7">
        <location>
            <begin position="685"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="154"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="167"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="221"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="235"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="101"/>
            <end position="108"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2076154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="843" mass="93040" checksum="370B2685BBEA19CF" modified="2022-05-25" version="1">MFRLFSSGVDDLVLVSSPTNGEITSQIGARFDRELIYTNIGEVLIAVNPYKQLPITGPEFIKMYQNSPGGNEAPPHIFALAERAYRRLVDENESQCVIISGESGAGKTVSAKLILQYVTAVSPNNSYGSSMGGSSQSGGGIPQYDGGSDDPNRGRGMPMMLPRGGLPQRGGGAPRGGGAPPPRGRAGPPPPVPSNRGPPPPVASGGGEGISRPVPPPTRGGARGGPAPRGGRGGGPPPRVAPPPSASSRRGGGTVDVEHIKKVILDSNPLMEAIGNAKTVRNDNSSRFGKYLEIQFDDNNAPVGGVISTFLLEKTRVTFQQKNERNFHVFYQMMAGLDQATKTEWGLTQATDFYYLAQSKCTTVEDVDDASDYRDVVAAMSTVGISSEETTEIFRILAAILHVGNIRFQGEAPAQVVDETPLQWAASLLGCDPQFLGQSLNHRQIQSGSVRHTQYAVPQNPDQSAGLRDALAKTLYERIFDFIVARINNAMQYHGNTKVIGVLDIYGFEIFERNSFEQFCINYVNERLQQIFIDLTVRGEQREYHEEGMKWKDISFFDNKIVVDLIDGNKPPGIMRVLDDVCKTVHAVDSATADIKFMEKLVHAIPNHPHLVGVNNGQFTIKHYAGDVEYSVEEFCFKNNDNLYASIVCCLQSSTFPFIAGLFPENMNDNKQAPTTASYKIRQSANYLVQRLSSCTPHYIRCIKPNDRKQPLNFVSSRVEHQVKYLGLLENIKVKRAGYAYRHLKDVFLGRFGKICEPPARNIQEFVEYVCKTLKDVSAEEFEEGKTKIFVRSPETIFMLEDQLMQKLDPIGYKQRVQAYKENEKLAQQKAGKHSMKTKCLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VAI7</accession>
    <name>A0A8J4VAI7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001510</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000036">
        <property type="protein sequence ID" value="KAF2077189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VAI7"/>
    <dbReference type="OrthoDB" id="2214470at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="164" mass="18755" checksum="518D18793C394184" modified="2022-05-25" version="1">MGYTTDFTGSFTLTPPLNQAQVDYLKAFSESRRMERSELKCKDVADPLREAVGLPVGEEGCYCVFSAADGNNGQTFDPTITDFNRPPKSQPGLWNQWLPNDEGTEIKWNGFEKFYNYIEWMDYINQNFLKPWGITIDGYISWAGEDSEDKGVLEAKQGEISFRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VAT9</accession>
    <name>A0A8J4VAT9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001203</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000027">
        <property type="protein sequence ID" value="KAF2077504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VAT9"/>
    <dbReference type="OrthoDB" id="329272at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052829">
        <property type="entry name" value="N-acetyltransferase_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43259:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43259">
        <property type="entry name" value="SPT10P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13673">
        <property type="entry name" value="Acetyltransf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="11"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="177" mass="20610" checksum="826F798DDAC367FD" modified="2022-05-25" version="1">MTIESTPTATANIELVSTDLGTTDFQDIRKIRYDVFVIEQECSEEEEWDEYDATSKHLLLKVDGVAVGCSRWRKVYYPKYEGNMECDVIKLERFAVLKEFRGKSFGKELVQQVIRNVYSETQGQYPLFMINAQQYVEKFYKGLGFETDANIPIFYEANIPHVRMIITKDAIKSIYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PK01</accession>
    <name>A0A8J4PK01_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001032">
        <property type="protein sequence ID" value="KAF2068363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PK01"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035285884" description="Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1" evidence="1">
        <location>
            <begin position="23"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="659" mass="73005" checksum="93C826DB0DABF004" modified="2022-05-25" version="1" fragment="single">MRGLHLSLLFVSLALLLSVVGSTVIDQVIYRPSNLDEYAPTTYCSYVFSLKLSNPNGIISIVAPSIAQINAVDQDSTIMYTTVTFTQNVGISGDLIVQTKDKFDTVSDVIIEQYECLATPTLQYDVSSKPYPTSTVSFGYDFKITTPLTKYLPARLSIGSTSSTQYRILTRANTKQVYTFFIQHTSPANGYADPFDLIIKVPTTLNIPITPLATISPFVGTSSAQSSAINSQYSMSIFNYENNLNGNTFLARKPSYSFYSHILAEGNSQKGTFLVHNNRRGVQNVYVLNVLDSFYSLQENNFVKLHEFSKSLPNIPSIAPTSYASNFVDSVAQPKTSFLNLVYNTNTKVYDRSHFVSIYKNLLEYNKFFTFPYAYSAGSITSGFKISFSYPTWGLFTGAMTYMVGSQSSQTVSKTSTNPVAADPLVVTNVALVGRNNQRLYFRLSISGSIPFDKIVSYSPLGAKAGITLATSANLILGTEYNGVYEFSHNIGGMDSVRVFNDMGIETLVNLPAGLNSAISLEHVKGIKFAKTNIDTTTATEPIRNRAYLDYDVPNLSFRFTIYESVLVINDVSNPQTYEVVYDSMLDKYVVDFEIYPRRPVFYQFVPYQLAPITSWSLDIMFPQDALKVTYENVDEMPPMVTSVSIVEGNTLVADSSAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PK44</accession>
    <name>A0A8J4PK44_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000986">
        <property type="protein sequence ID" value="KAF2068438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PK44"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF29">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="138" mass="15583" checksum="20EEAF90E8FB7C18" modified="2022-05-25" version="1" fragment="single">VKLQVDDHSLYGRFIKRGIIDGRISLITNDLFSNLDQSNQFNNIQSYIGINIRSYRRLVQLDPDFSVLIDQRPAQDSNNPLCSKSKRKLSTAQLAGIIIGCVAFAAVIIGSISFVIYKKKKQNTFNKKFEQKLSRMGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PK82</accession>
    <name>A0A8J4PK82_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009997</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000879">
        <property type="protein sequence ID" value="KAF2068679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PK82"/>
    <dbReference type="OrthoDB" id="659430at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1241" mass="140209" checksum="B95A1BE6C5985A01" modified="2022-05-25" version="1">MAFFNQWTTPSIDKGWAITNRDKENNRDIFELELEFDALGRIKQQSLAAKIPDGTLTLCLCIKDLEEKDTERNITDRYVDLHGMVIPHSVVHLVLVDSLYLDINVVYQGHWIPSSVTMVSVPSLPFVPVSLIPESVRYLRVLNQRRPIEIGMVPSTIEHLYLCLERFPPPLAHDLPNSINELVVYSLLPFEEINIRPTFTAPIHVLSKYVYDRQVIDKLKLHLNIKSATFIDTQVGAITLPPTLTALVIEKGINNNSLSALHPLPPQLKYLSLPSSYQHPIYQHQYLPTSTTHLELSLIVTSRYSHFTNNWLGRIGYLPRSITHIKINILFGDDDSRAGEDSLPSGMKIVLPSSIQAVCLVPDNNNYRHHITIIDSKDYYSTTPTPPKIRDGLKELVVPFSFNQIIETNTLPTTLQSLSILNTNYKHPLNSDNIPTSLTSFTCNCYCVQLDLSAHESIKDLAIDVNSVIKYPPNLESLHLKRATTWGERDDYLNIVNKDLIKKVVLQSFDSDRLDQFLSLGLPSLEYLKSSSSLKSVVQASVQELDISDIYCTCQQHLLPTIKDIHLQLNPDILKAISLPTASIGNNDINYVSNQGPMVDSFFKLWRNVYMKSRILDSMYDTTKSTLRLNIKTGTLDHMDRFKDYNIIIDHVNQFVPDLLHFNKADQIDMEAIRAHDNLAQEIPKSIKRIKTDLVKGVIPDWITHLSMRAWKYLRNVGVGEIPPSVTNLTVSSKFEEEEPDFSKIPSSVRFLTLDRLKSHQARSIPASITHLTLVKQPIISLDQIEIPETVAKIELKSLVFQTHPKDDPSYDIPFHVARRLVNGVYYIYSKSKNRTAIPNNTTHLFWLDGAQHIDNNDVAIPSSVHTLVLGEEFNAVILSLPSTITQMVFNGVFKPVDAICFPPHIKYLRFSSASKVIEEHHLPKGLTHLVVDHYVKIASLPQSVRHLQFLHNGYDGVIPPQVQRLKVESRFGTTYIPPTIKSFITSSYDKVTFDNTKSYQEFTQEKIILPFTTNANIHLHALYGFNTFIMPHSLGNNIKSISLGNQFNQVILPGTFPNSVEHFEFGNEFNQALNKSLLPPSLQTLALGRDFNQPLDDLFPPTLTELSLNMTSMPSISSSTQFPPNLKKLAIPWYDGVLDVVPTTVNHLEFNANVAQKGKDKANQVYLFPIESLPPHITKLVLNDSMCIQGYNIIPPTIKSIKLCHSIAPGTKIPNTIESVVLPSSFNQPLDTILQTVDDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKH6</accession>
    <name>A0A8J4PKH6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Membrane insertase YidC/Oxa/ALB C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_009167</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the OXA1/ALB3/YidC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000647">
        <property type="protein sequence ID" value="KAF2069520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKH6"/>
    <dbReference type="OrthoDB" id="2148490at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20069">
        <property type="entry name" value="5TM_Oxa1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="YidC/Oxa/ALB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428:SF65">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX18, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="OXA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="311"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Membrane insertase YidC/Oxa/ALB C-terminal" evidence="8">
        <location>
            <begin position="145"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="358"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2069520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="388" mass="43908" checksum="ACDAEB865C61D4F6" modified="2022-05-25" version="1">MIQGSKLLRFSQSINRSGLGFTTVNSNTSNSILKSTLKLGSSNIKSSSSSIPLSLFNNNKNIHKAFKPLFASSQSIRFYTTENNNNETLAETTNNLITNNADQAQATIVENITIPSQNPVIQFFETMNMKVIGQMEQLTETGIPWLVIIAGVAVTLRSITLPLTIKSMKIGQTMKEIKEEIDVKYAHLNDGTSEGKLQLMSVTNQVMAQRGVSMFSSMKYGFMQMPMIIYPFYIIKELCESGKISEAGYLWFTNLSVQDPYYILPVATCLFQFLSVHFTVKDSPPLMRYIMYSVSFLPLIFTVQFAAGLNVYWLFNAMLFALSNYLLRETKLATMVGLQLYKPKLTGPVIQYAPSPIIKKEQNQLAKEILEKRQLINEKKEELRKRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKR2</accession>
    <name>A0A8J4PKR2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LYR motif-containing protein 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_009865</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000834">
        <property type="protein sequence ID" value="KAF2068813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKR2"/>
    <dbReference type="OrthoDB" id="529194at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20267">
        <property type="entry name" value="Complex1_LYR_LYRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045298">
        <property type="entry name" value="Complex1_LYR_LYRM7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050435">
        <property type="entry name" value="MZM1/LYRM7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46749">
        <property type="entry name" value="COMPLEX III ASSEMBLY FACTOR LYRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46749:SF1">
        <property type="entry name" value="COMPLEX III ASSEMBLY FACTOR LYRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2068813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="15596" checksum="6109BBFE7AFA2FE6" modified="2022-05-25" version="1">MQRAKVIKSYINLLRTEKKVFGNDPNALIHVMNQTRTQYRENKDQQDETKVNELVDHANAVSDFLVKNLLQATRKNEKTFELKFNEHTEYYKQYEFINDQPPARKKRGKIAIPEEESGCCGGGCGKAPSPSSECSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKY3</accession>
    <name>A0A8J4PKY3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009745</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000794">
        <property type="protein sequence ID" value="KAF2068932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKY3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="402" mass="44785" checksum="A49574774DF97B5B" modified="2022-05-25" version="1" fragment="single">CAFAAEPPFTFSLSGSCAYSTSLPTAYAGDLSEFKFTTNDGNVTLEKAYRIWDTTYQPTLFAYLTVDYQPPVIFHYKGVSYDITRPSPGCVLVNKAVLKESKGQNLEISILISEPENGESLEVYVDDVKSGTFKVKLDTNNIIEPFSLYFPYTQYTHTVRFNRLSLDQSFSAKITYAKPEIISQECVRGFQKINADQPPHIINTCQFVGNKFHSFDLLSGVGIVGNARVSLTSISLNLTGNLVLGAPLTLDTKDGPYSYYNDFTNDTFRPQPRGSSSTFIYGKDANGFRTAIVKGSYLDAIAIDQVQVLDLTNNEYFNVGNFCRYSAETSLEDGYQEMLSCLVPTLKNYEKAAILLTPIYYPNQTLIFHTEKMASSSSYTTPSLSSLLFTLSLLSILFLKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL97</accession>
    <name>A0A8J4PL97_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kri1-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_009679</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the KRI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000775">
        <property type="protein sequence ID" value="KAF2069003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL97"/>
    <dbReference type="OrthoDB" id="10252032at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018034">
        <property type="entry name" value="Kri1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024626">
        <property type="entry name" value="Kri1-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14490:SF5">
        <property type="entry name" value="PROTEIN KRI1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14490">
        <property type="entry name" value="ZINC FINGER, ZZ TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12936">
        <property type="entry name" value="Kri1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kri1-like C-terminal" evidence="3">
        <location>
            <begin position="494"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="69"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="159"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="321"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="419"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="576"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="81"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="127"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="201"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="213"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="335"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="423"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="582"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="614"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="651"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="663"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="691"/>
            <end position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2069003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="701" mass="82565" checksum="778832154AC9BDE5" modified="2022-05-25" version="1">MSDSESDHSSSSSSSDEEVIQKNKMIDSSSDEEEQEKKKTLLDDDDSDDDNNEKELKVNPKFVKFYEEKKKREEECSAAAEAEDEESEDDDAVLDSKAWNDDFLTLFPMLKSKSQKIYNKDVTFFKDDDEEEDDDEDQEESSKKKKIYTLRELQNEKIIKSMDKDQYESDEEEEEKDFDSLPHVKQQAKLKKDFLKAASNDESDDDFDDGEDLLMRREKSREDQDAEDKEFEDFKNRITTKKGDLIKGEEQVLNYWKSKEENENEDDQFLKDYILSQKWHSDKGKLPTYEEIMKELDQDEGEVHRQEDFERNFNFRYEEPGAAIIVGHQRNPQDSVRRKDTKRKEKRDAKKEKKEQKKKQKDEEIKRIKNIKKKEILEKIKEINSITGSSLFDLDSVDIKKIDPELMVKTIGKKKKEYQQEYQEQEQEDLGEEYYDENQGYDENDQGYDENQGYDENDQGGMDLNKLIESGYFDDQVEDDNEVWSDERMSQERKELEDLLDKYYGLDYQDMIDDTPVRFKYTKVAPDTTITIDDILEKEDRELDKMLPMGKIATYFNPSFNYRQKAEKRYFDQKKNGHLTWSNNNNSNNSNSNSNTSKFNNKQQINKNDKFSKFSKNVSSGKFNKTNNYNNNENNNSNNSTTKTTTPQFKQQDKLNKEPFKNTTTTTTTTVTPPTESADGVKKRKAKNKNKISANKAQKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLA9</accession>
    <name>A0A8J4PLA9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008689</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000561">
        <property type="protein sequence ID" value="KAF2069987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLA9"/>
    <dbReference type="OrthoDB" id="20560at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903599">
        <property type="term" value="P:positive regulation of autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047118">
        <property type="entry name" value="Fbxo7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15537">
        <property type="entry name" value="F-BOX ONLY PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15537:SF2">
        <property type="entry name" value="F-BOX ONLY PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="67"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="310"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="389"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="50"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="310"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="397"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="441"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="456"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="574" mass="65865" checksum="0A112646AE6C885F" modified="2022-05-25" version="1">MISNNNNNSNNNNNNNNSNSGNSGNSMINNIEKQLQKNNVSRINQLKQKSNSNNINNNSSNNSNNSPTNLVDLPDEILLKIFKYFDVNSLVTISQTSNFLNSVSCNHDLWKQLFYRKWGPLFHLVPVCTIKCCHQFFSNPYTYLEPIHQLFSLSFYKSLLIPKELISLITTSYSFRHLFKFRSQLFTYYRPGLINGLELLKNTVDMKDVWMKIDDPILLTSINTVTQSKIEKAIIDNQPTTTVTCDLNEIQLLSQTTLCIRVSKIFPTRILKQLLNLIIDYEKIPLWDVSLRHCTGMVVKSPDFRVYPNNCHSSNSNNDNSNNNNINNNESDNNQDILCDNDRNHIRKVDILHKQIIGNLYMTYSRMIFSRSDENIKFLNGTLENIHIEDSSDKNKNNNSNNNSNNNNNQNNNNNNNNNNNNNNNTTITGGEIETIKKRKRNDDTCIIEEEKSKSSRSTNTLSASSINSKEEETIIKHPIYIVQNSVPFKTTDNENNLDTFKLECFGSGFIFEPLNESGKETKLTYLLQMGKQDWMEDVLELIDEMAVSRNRSINSLISHAYQLSRKPLVATSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLU0</accession>
    <name>A0A8J4PLU0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010149</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000946">
        <property type="protein sequence ID" value="KAF2068526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLU0"/>
    <dbReference type="OrthoDB" id="10255646at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07821">
        <property type="entry name" value="PYR_PYL_RCAR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019587">
        <property type="entry name" value="Polyketide_cyclase/dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39332">
        <property type="entry name" value="BLL4707 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39332:SF7">
        <property type="entry name" value="SRPBCC FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10604">
        <property type="entry name" value="Polyketide_cyc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="145" mass="16543" checksum="996EB5A0CC8A94AD" modified="2022-05-25" version="1">MSTRVFQSSVIDQSIETVWATMRSFTFPEKVFPTIESATMEDGAQPTCVGAVRTLKWKTGETRSQRLLELSDLSHTIVYELIDSSPPTEVTAYITSIKLCRITDSNKTLITWEGEFSSDVKSDVIQFEQKSFQLNLQDLKRFFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMM5</accession>
    <name>A0A8J4PMM5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Brix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_008779</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for biogenesis of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the BRX1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000576">
        <property type="protein sequence ID" value="KAF2069908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMM5"/>
    <dbReference type="OrthoDB" id="1638493at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10480:FF:000003">
        <property type="entry name" value="Ribosome biogenesis protein BRX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10480">
        <property type="entry name" value="Probable brix-domain ribosomal biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026532">
        <property type="entry name" value="BRX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13634">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13634:SF0">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BRX1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Brix" evidence="6">
        <location>
            <begin position="66"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2069908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="317" mass="36464" checksum="E5B681DBE56E0047" modified="2022-05-25" version="1">MVKPKNTTTTTATKKVEKKVAPVKKDTVAKAAPKTTTTTTTTTAPITNPITTPIDTSKDQSIYTKKRVLFTSTRGIGSKYRHLMTDLLNLIPHSKKEDKIDDKKTLSLVNEICELKSCNYAVLFDVRKGTDLYLWMAKTPLGPTVKFHVTNVHTLDELQMTGNCLKGSRPFLHFDKSFDTQPHLQLIKELLTQIYATPKGHVKSKPFFDHVFSFYFQDNRIWFRNYQISDQEFKKESLLTEIGPRMILNVEKIFSEGFGGQVLYSNPNFIAPNKNRLDQKLAKSNKYIKRKYQKEQSTERQKVSWIEPSEVDTIFEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN16</accession>
    <name>A0A8J4PN16_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007759</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000432">
        <property type="protein sequence ID" value="KAF2070915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN16"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="70" mass="7606" checksum="509345151CEF0D08" modified="2022-05-25" version="1">MTLLKSMTSLFNQFSSSNETSISLSGSSLTIQSNIYSTIDNGLNQISAKLPGNNDPVSRKLGPSFKQFFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN46</accession>
    <name>A0A8J4PN46_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_008324</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000503">
        <property type="protein sequence ID" value="KAF2070353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2150267at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038039">
        <property type="term" value="C:G protein-coupled receptor heterodimeric complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004965">
        <property type="term" value="F:G protein-coupled GABA receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15047">
        <property type="entry name" value="7tmC_GABA-B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002455">
        <property type="entry name" value="GPCR3_GABA-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519">
        <property type="entry name" value="GABA-B RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519:SF66">
        <property type="entry name" value="METABOTROPIC GLUTAMATE RECEPTOR-LIKE PROTEIN P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="741"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="776"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="847"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="894"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="937"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="962"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="11">
        <location>
            <begin position="739"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1055"/>
            <end position="1474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1055"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1067"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1109"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1132"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1155"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1175"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1205"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1217"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1274"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1285"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1313"/>
            <end position="1327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1337"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1351"/>
            <end position="1382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1383"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1417"/>
            <end position="1450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1463"/>
            <end position="1474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2070353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1474" mass="163375" checksum="9A86BF623110A71A" modified="2022-05-25" version="1">MNTLNLDKDPQQQQKQEKQKRIYRFTFSSIWIMKYNILVLLIIFYSFGFILSDDVFYLKPKGSGSTGDCGYRADNPCDDLTTLLPRIRVNFNTTVILESGIYTGNFDYQLTNYTLTIIGKGPTNTIIDLERKQRFMTITITTLNIYGVSFINGFTNESLDGGAAISSVYSFVCLENVHFRDCSSEAPGGALNIYNSEANMTNCRFINNRSNSGGAIIFYLTNSRVYDCVFKENSAKQEGKGGAMEIFISTIDIINSVFAYNLSPYSGAIDMVGGNLTVRSSIFVKNQSNIGGAFGSYFISQSSFVSCIFEKNSANNTGGFGFLTAQSLSIFSLCKFIDNFAPNSAILETHSISPVLFESCTFRGNVETTVQISLFDNYCFMKAQDCYFHNLSGVLIYADSVSNILIEGSKFTDIQNRVIDVGNQADILLISSVFKDIEISDYLIALSNGKIITYDVLFDNITSLAIIRSTLGGTIDIVNTTMSYNNVTYGLIYIEYKYTLYIERSFFLYNSGSFRGCIVAADHTGSEIIFKSVFIGNQSPYGPIIFFSTPTIARITEEPSMFTDITFIDNYASIAGALVYFSDNISIPDISCTNCTYTNNTARYGELVNSSFYKFNVSMPTILYPHQEIVILVNALDYFDNLIRGSSDLGFFAIACSDSYVEGSLFSVLNENGTATFNVRISSFPGTTCNLTILSVPTPSKLGPTVILITFIDCNSDRELVEVNSIFYCLCTVDTPTFVKFVLGVIALILALMLIASGVISLKYRKKRVIRYSNPLFLIIILIGCLVYLVLIPVMYSTSSASCKIRFPIIIFALSMVSSSVFVKQFRIWKLIKDIQLLRETNVENKYLLQFVSLLMIVPVLIIIVSFFALPTKETVKYDTEKVTITHYCTFDYYFIYIIIFLIYQMGILFFGCYLVIVCRKFRSVPGTFNEATYIGILIYNYTVVIIVAVPLSFVFHQNPLANFLILSISCLVFVFSTIALLFIPKFHFLIRNKVIISSLEKLIKEQEVIVQRNKDILSFYELYLSEERPNGLENPSTPYQLRNRRPNQQSNLATFSSEDSSIATNSSSSPYTLSSSIDSGDDDDSPDFNQHLYPITNTIISKRRAKEGGGGGSGSGGGSKSKRHKKKKDINNNNYNSNTGTTTRKRSKGIIAATTTSSRSLPSSPNPINRDENTSSPTNTNTSKPPQDAVGKLKSKSISFLSLPNSPHMNRNQDTPSSSSSSASPSSLYMSPPAVSSSSPSQLTSPTTSTSTISSDNSPDLNAEINPYLKPNSMKNITRSTTNQQQQQQQQQQPKPKSKTKHISDKNQRKPSTTSNDINNNNNSSNQGVKLKKQKSISSNTSSISSDNSPDLNQDFNPYLQTNSTKSVGTVTQRKTPTIQKNNNSSSNNNNNNREIRNPLLSPLSISQPIVRDEPPSVSSPSPSPSTQTSPTTTTTTTTTSTISSDNSPDLNAEVNPYLKPNSMNNITNPKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN61</accession>
    <name>A0A8J4PN61_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000659">
        <property type="protein sequence ID" value="KAF2069456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN61"/>
    <dbReference type="OrthoDB" id="19902at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040310">
        <property type="entry name" value="DDB_G0292248"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31648:SF4">
        <property type="entry name" value="CARBOHYDRATE BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31648">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25544">
        <property type="entry name" value="Ependymin_amoebozoa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035274228" evidence="1">
        <location>
            <begin position="18"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="234" mass="26115" checksum="3C61F14DBB905761" modified="2022-05-25" version="1">MKVICLLLFFFVCGSLAHHCKNTTGYNTRFSLSAYNTYFPGKNGMEAMSFFECGSINIDFKQKQMAVSFHIIISGHPTIDGSLFAFGQNNTMYVTMNNGTACKKFPNSYPFPSGWPNVTNWGKTKIGQTSVDILDIPASPNGPMTNQTVFYDHKHCACLSTIIRNADISNAGVSTMLFFNFENKPNPDGFNLPSSCLNSDEHVDILLNRKRGDPTAEEQFQQSKALHYILQAFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNW1</accession>
    <name>A0A8J4PNW1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007298</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000381">
        <property type="protein sequence ID" value="KAF2071382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNW1"/>
    <dbReference type="OrthoDB" id="22671at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="519" mass="58467" checksum="09C7348DF675B49D" modified="2022-05-25" version="1">MSSFFKINKHKYIRNIISNNILCGKTIQGSLSFIEKEHNILGILNHSLFNITIQLEINSTEQFNHYLESPYRHIINSLVLDIDIEPKETSEFLDLLNEIPSQVTNLDISSKLSTLLPNTSFSKNTTLKNLLIDYEGQVKENCLPIGLQHLNIVTHGVPLAKEIFDQQKQLKSLDILVSSSWNQAFSQFNEKSSLEINVRMEFIASVVISELVNFKNLNITTLDLFNISFTICSPGDLPSSLTKLKMSSIGNLVEGFIPNGVKILTLYNFNRDFNSTHLPSSLYKLTLTRFSQPIRKGMIPDGLKILKLPFFSSILQHQSLPDSIEYLSLNSYTNAILPGLIPKSIKTLHLHSYTEQIRLGIIPNTCKQLSLLSYTGNLEEGVLPIGIESLTIRNGVLEPIYLPNTLTDMTILRYDESKSTNLLQHCKNLQKLYIISSIKSISAQIPNTVTHLSLAFNVESISESSIPSSVEILEIQSSKFDFQSIKNLTTIKTLKSKTYPSVLPKSLLNLIVDNKFQTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP08</accession>
    <name>A0A8J4PP08_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007203</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000370">
        <property type="protein sequence ID" value="KAF2071487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP08"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="685" mass="78505" checksum="584D4CBB92E9FDEE" modified="2022-05-25" version="1">MNKEDLDAKPYRELVNLAKKLGVKANGKKEEIIDRIVAHSNDDSDSNDDNTPVVKKIKSSTIKKVDIKRPEPSVVVKVVEERESNSQEEQKNNCKRKRDVVDKLSLSTKATSTKKAQPVTQIIRKDSKLDDIFFTIWRDRCLQQKIFLSTIDYVLLKNLIQLDKQYSFLSKLNNNNIPVAYHIKDLDHYHIYNAHQHKSLITHIVVSSTFYQNHLHLIQQQQKQPILFGISISSQNNDPVLNVKDLPNSIQYYQYKNKKQQITKDLIPQSITSLDIEISSIKPGVIPNRVKTLVLGLARSNLDIKCFPKSLTSATIYDFCFKNTHKINLDLLPPRLLYLNLTKIEGEITGNGPKSLKYFVGRTEHLDVPSSVTQLKRVSHTNLNDVHLPPNLTFLGNHYPINFGPNVWSIPENTLSRLKALLLDNIPHSCITKENCPPGILYYRNRNYKLNNHVPYIPSSATKVSISICNEMERLPIIPSTVTDLAMENPNGQVLFTKSIKNGYFANNIVKLSLKYDLELTPGIIPSSVTDLKLIYSKPIKADIIPPSVVKLCLISRETVKPIILPNSITSLSFENDIQVYSDMVIPKSVETLKIFNQLNFQTFPFQLFKNITRLNTRGIDDAKSNFIKTFGITTDNNYNEKLFPSNIKLVEFRYSNINYYIIDKKVYKEENHVKNNHFIKFDNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPD2</accession>
    <name>A0A8J4PPD2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TPR repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_007655</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000420">
        <property type="protein sequence ID" value="KAF2071034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPD2"/>
    <dbReference type="OrthoDB" id="10268002at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="301"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="374"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2071034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="585" mass="68061" checksum="31677281C5726E72" modified="2022-05-25" version="1">MNSIQRLTRFKYISRLSISNSITQGNSKIKSISNNSCNSNKTKIFSPINSNNIYCFKHYFTSSKSLFSEQQQQQQQQEIDVKYQSLIKDAIEQFKGREIIITVQLLDQAIELCDNKPDAYLLRADIKGEFHKIFPSALHKDAIIQDYETAVNLVPQNKKAYVYYTISKYIGKLIDASFEQMIFYLDKAIELDPDNHDYLADKIIFASFLNRVEEIIEPSKKLVKDGSKYGPIALAFISSSQENYPQAIQLIQSVIDSEVNLPRQDKFKGVPVYYGRLIVGTDRQGFIRIRERYFESNNILFDSYVALGIVHSYQNEIDLSMDYYKKALEILPYAYQFHGILASTTFQLGRFEETVEHCNNFFKHDPEFDSPYANQALTDRGSAYYNLGDLQQAYFDLQISLKKNPTEKFTRENIFNRSNPTLIMIYCLNYFIQTHKGLDKSKYNIEGMIENIDSDGVLSVEPKNIQQLLITTRLWFSIYSDISKSIESGLVKCSEEESSNLYFREGLLYHLADYLVDSMYLNYLENHSDIAFNGNTENENFPALLDFISHLPYRESKQLPERQEAHKVLFEVYQEFMKHTQENDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPF8</accession>
    <name>A0A8J4PPF8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">maleylacetoacetate isomerase</fullName>
            <ecNumber evidence="5">5.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_006951</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-maleylacetoacetate = 4-fumarylacetoacetate</text>
            <dbReference type="Rhea" id="RHEA:14817"/>
            <dbReference type="ChEBI" id="CHEBI:17105"/>
            <dbReference type="ChEBI" id="CHEBI:18034"/>
            <dbReference type="EC" id="5.2.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 5/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GST superfamily. Zeta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.2" evidence="5"/>
    <dbReference type="EMBL" id="AJWJ01000344">
        <property type="protein sequence ID" value="KAF2071727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPF8"/>
    <dbReference type="OrthoDB" id="202840at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016034">
        <property type="term" value="F:maleylacetoacetate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006572">
        <property type="term" value="P:L-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03191">
        <property type="entry name" value="GST_C_Zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03042">
        <property type="entry name" value="GST_N_Zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000017">
        <property type="entry name" value="Maleylacetoacetate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000041">
        <property type="entry name" value="Maleylacetoacetate isomerase isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005955">
        <property type="entry name" value="GST_Zeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034330">
        <property type="entry name" value="GST_Zeta_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034333">
        <property type="entry name" value="GST_Zeta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01262">
        <property type="entry name" value="maiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42673">
        <property type="entry name" value="MALEYLACETOACETATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42673:SF4">
        <property type="entry name" value="MALEYLACETOACETATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14497">
        <property type="entry name" value="GST_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0828">Tyrosine catabolism</keyword>
    <feature type="domain" description="GST N-terminal" evidence="10">
        <location>
            <begin position="7"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="11">
        <location>
            <begin position="95"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2071727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="219" mass="24702" checksum="838819760C172C7B" modified="2022-05-25" version="1">MQNIDKDTTVLFSYWRSSCSWRVRIALALKKIKFEYKAIHLLKEGGQQKSDDYSKLNPMKVVPTLVIDGNVLGQSLAILEYLEETRPEVPLLPKSAADRAIVRQMMQIIGSDIQPLQNLKVLQEIGTLAGDDKKGAWAKKWIANGFDGLEKLLVKHSGKYCFGDQITFIDLCLPAQVYNANRFNVDMTPYPTISRISDALSQLPEFEFAAPHNQPDVEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPK2</accession>
    <name>A0A8J4PPK2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_006886</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="7">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000337">
        <property type="protein sequence ID" value="KAF2071802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPK2"/>
    <dbReference type="OrthoDB" id="409122at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008240">
        <property type="term" value="F:tripeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04056">
        <property type="entry name" value="Peptidases_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11377">
        <property type="entry name" value="Pro-peptidase_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000040">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015366">
        <property type="entry name" value="S53_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030400">
        <property type="entry name" value="Sedolisin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050819">
        <property type="entry name" value="Tripeptidyl-peptidase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218">
        <property type="entry name" value="PROTEASE S8 TRIPEPTIDYL PEPTIDASE I CLN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218:SF15">
        <property type="entry name" value="TRIPEPTIDYL-PEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09286">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00944">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51695">
        <property type="entry name" value="SEDOLISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035278632" description="Peptidase S53 domain-containing protein" evidence="8">
        <location>
            <begin position="19"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S53" evidence="9">
        <location>
            <begin position="199"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="269"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="607"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="652"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="653"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="675"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="677"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2071802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="697" mass="75318" checksum="26220891C494EC5D" modified="2022-05-25" version="1">MSKTLLLVFLGIVALAASFRHENVMIASVDSVPHHFMTIKPAVSSDSVHFKILLHQQNLDKLEALFWEVSNPKSSLYKNFLNKQQVDSLVAPSQDAVMEVAHWLIKHNIKKSEIDVNTDYIAVSTNVEKASKLFGSEFAVYQSRITKNTRVRLNGPAFVPFVVSQHIDFIVGLSDFIEDNKMVQSMRTQERMMGTASATITPAFIRKFYGVGNAIGTNGANFQSIAAFTDYFSTPALEYFDKQFNIDPATVKVIKQGQDCISQGCDSMESNLDVQYITSIGNNITTLFLSEPNGAWTLDWALDIQKQHPMPLVSSISYGWAEIEQCEITSDCSTLGVSSAQYVARTNVEFQKIGIQGSSILVSSGDDGAPSFGGASGNCPIDNSIYCPIGGCSYKSTQCSEVTITSSNGTKCFFPMGLQSNACQEVLGSDEGNAVINAYFQSQSGSCAVHLDQDSEQNYHLSTTCKCSQLKTFKKSGYTIVGYEFDESAGTVFQPDYPTSSPYVTSVGATQILTNNQEIVCSVSTGAIITGGGGFAITQAQPAYQADAVNAYLNSGAQGLPSSQYYNASNRAYPDITLVGHAYQIGYTKGQNTEQCPCAITSVDGTSCSSPTLAGLISLINDKLLNAGHKQLGFLNPLLYQAAAAQPNVFNDITSGNNNCNRAYCCEYGFPATKGYDAASGLGSLNFANFESYVFNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPU2</accession>
    <name>A0A8J4PPU2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TRAF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_006684</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probable adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000319">
        <property type="protein sequence ID" value="KAF2071995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPU2"/>
    <dbReference type="OrthoDB" id="1630758at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001293">
        <property type="entry name" value="Znf_TRAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131">
        <property type="entry name" value="TNF RECEPTOR ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131:SF94">
        <property type="entry name" value="TNF RECEPTOR-ASSOCIATED FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02176">
        <property type="entry name" value="zf-TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50145">
        <property type="entry name" value="ZF_TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TRAF-type" evidence="9">
        <location>
            <begin position="71"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TRAF-type" evidence="8">
        <location>
            <begin position="71"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2071995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="249" mass="29030" checksum="269B6F4D6335D682" modified="2022-05-25" version="1">MMKCPYSNLCENMNYGTFIDKHSRECEYRSIKCQCSVIMKFNEFENHQKQCPKSKISCPHCQTVFVRDSNHLETCDEMNISCHQCKTKIKRKEITDHIRDVCPETMTTCPLKYLGCGGSFFKKDKMAHLKSDQHINVCMDISDQPVFMTTLERGTFETKFQFLGINSSFSYQHGNQVLDITFDNLNDQPFAKDYDFRISIYDKNNNILDPGDKYCLSEITGILSRHKQNQAFQSIKLTIFKKNQSPQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ02</accession>
    <name>A0A8J4PQ02_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Laminin EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006786</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000328">
        <property type="protein sequence ID" value="KAF2071907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ02"/>
    <dbReference type="OrthoDB" id="18757at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053369">
        <property type="entry name" value="SrfA-induced_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32256">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32256:SF6">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035261149" description="Laminin EGF-like domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="2">
        <location>
            <begin position="323"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="2">
        <location>
            <begin position="376"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="415" mass="45515" checksum="DC81B5B54817F6AA" modified="2022-05-25" version="1">MKTLILIVLIVLCFYRVESKKCKASWECGNMPKAICQNNINLFSGIGVDDINNKVYIMGNFNSSGVPYGSTYIASVPLSGGSLSLEYPILSDIQTGAGFRTAYRLFKYLSKSKLMYTQNAERLAPSIGPYFTNGTLNGIWYPRGFSLAADFDEVAQMTLTCEFSITKYPMIAQDTSIYQNRIELYRTLCQGIGKVGSSFYAVRYNPSGSGSQFFKGDINCVNCDPNTLVILFTDAAQVTGFEVTSSHIFYSSKWYDGSNEFKGIFQVPLSGDLSQKKVLVSDSVDSMELSNNVIYYITGGKVKSVSIASGAVSTLYDNTNSNSEGECACAPGYTGQDCSQCAGDEVWDSNGNPKCLISPPGQKACIYDWMCNQPYGYCDGSVCRCKYNFSGPKCDQCVNGLIKWDSYGNPSCEML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQB5</accession>
    <name>A0A8J4PQB5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoglycerate mutase</fullName>
            <ecNumber evidence="8">5.4.2.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_008501</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.11" evidence="8"/>
    <dbReference type="EMBL" id="AJWJ01000530">
        <property type="protein sequence ID" value="KAF2070174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQB5"/>
    <dbReference type="OrthoDB" id="354304at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004619">
        <property type="term" value="F:phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000003">
        <property type="entry name" value="2,3-bisphosphoglycerate-dependent phosphoglycerate mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01039">
        <property type="entry name" value="PGAM_GpmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005952">
        <property type="entry name" value="Phosphogly_mut1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01258">
        <property type="entry name" value="pgm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010713">
        <property type="entry name" value="PRK14115.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11931">
        <property type="entry name" value="PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000709">
        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="4 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="5">
        <location>
            <position position="10"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="9"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="22"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="88"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="115"/>
            <end position="116"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="184"/>
            <end position="185"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2070174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="249" mass="28421" checksum="2B8DA6B50FEE2026" modified="2022-05-25" version="1">MVYKLVLVRHGESTWNQENRFTGWTDVDLSQKGVLEAHDGGKRLREAGFTFDIAYTSVLKRAIRTLWIMLDELNLCYIPVEKNWRLNERMYGALQGLNKSDTAAKYGENQVLIWRRAYDIPPPALEESDDRFPGKDARYKDLSPSDLPKTECLKDTVDRFLPLWDETISKTIKSGKKVLIAAHGNSIRALIKYLDNVSDEKIVSMEIPTGIPLVYELDENLKPIKSYFLADNDELLKAQQAVANQGKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQH5</accession>
    <name>A0A8J4PQH5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007728</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000428">
        <property type="protein sequence ID" value="KAF2070955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQH5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550:SF11">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="168" mass="19731" checksum="484DBDF021034F0C" modified="2022-05-25" version="1">MMMGSIDTLYRDVFGNVYLSSLIFKCVHQIQINDFSFKYKDIWGVGWMITYGHIGLLRDKIKNDANRLYVCVEELFSIIANYKDISIFIALFELHKTAVLQYMQNNENYRQVIDKVRSLKIFSYLHNNGHLRTLETPPALSDYSIVEYLTKLGYLKISYLRPTDSSFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQP6</accession>
    <name>A0A8J4PQP6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008239</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000491">
        <property type="protein sequence ID" value="KAF2070444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQP6"/>
    <dbReference type="OrthoDB" id="185175at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005547">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000286">
        <property type="entry name" value="PH-protein kinase domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051707">
        <property type="entry name" value="PI-Interact_SigTrans_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14336">
        <property type="entry name" value="TANDEM PH DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="16"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="268" mass="30937" checksum="8CA8930C92E7392C" modified="2022-05-25" version="1">MTQNVLRESRDEKRKSTIKEGSVTKQGGRIKNWKKRWCVLNEEGLHYFKAQNSIEKGSIHLENILACASEDKPSTKRKYCFKVKTVERTYRICATDSLDRDEWVESITKLLKTKDIGQQKQNGDLKSSNSVDISKIKIDDDDSESSAADSSTPTSYSNSFLKENPSKTIVLTPIMISLKDKFALNTFGPELNRKKDLLSLEETERVLNLLQSHIENEIEIANVDRDREIKLIIKQINKLKDQELESSERLYNQKRLEILNEINKKSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQS7</accession>
    <name>A0A8J4PQS7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_006800</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000330">
        <property type="protein sequence ID" value="KAF2071873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQS7"/>
    <dbReference type="OrthoDB" id="6431331at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046464">
        <property type="term" value="P:acylglycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050266">
        <property type="entry name" value="AB_hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798:SF33">
        <property type="entry name" value="HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G14860)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798">
        <property type="entry name" value="MONOACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="171"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="443"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="443"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="464"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="493"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2071873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="503" mass="57602" checksum="656F66CE9F5B975B" modified="2022-05-25" version="1">MDNSNTNTTSNKKNKDVVKQQQEDDTPTKSSTTTSMKKRIIKDDDDEITTTPSGTVSSQSIESKKPKQPQQTTNNAGCIRVFGLLLVTLITFVLWSSFNQVDYKKMDSSMFIKEMPAPLLSHFQAGDLIPVFDRSTGNTFNVFMIQNTYIKDDQNAHLTLATSTNIQDNEIVLFIHGQATTSFVFRRELQYYQKENIQPILIDLPCFGFSDSLDDCSYNNIAKRIPDIMDALQIKKPVHLVSYDTGAVIASKLYLSFPNRLKSISFADPYIDMTKERLPFYYKVATYNKQFISGPLIRFITAPFMSWIRYLYVSNVYKYEISREELDSYFYSIRYKNNVNNYIDTLVQSDLTYSQTYQITNSKLSISKAIPRLVFISTFLLPSYQSQFFQTKFQDLETQSMVESKYSLFEQSPNTLTSALVMLIDETDPNLRKKKEVILEDHQDHDHDHGHDHGHDHGHDHGHSHGHSHAHSHGHSHGHGHGHSHGHDHGHGNQYGMGHNYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQZ6</accession>
    <name>A0A8J4PQZ6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_007316</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000382">
        <property type="protein sequence ID" value="KAF2071376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQZ6"/>
    <dbReference type="OrthoDB" id="10256233at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47960">
        <property type="entry name" value="DEAD-BOX ATP-DEPENDENT RNA HELICASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="212"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="500"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="56"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="278"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="118"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="278"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2071376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="713" mass="80776" checksum="9B9373B598022DFB" modified="2022-05-25" version="1">MLRYFIDKTYQFKLNTLNNNAVFSSLSSLNNRFYVKTTTNHKDNISTLGKREKINRDGLDKKGKSQHLRNNKSISNTPFKVGESKKHEGNRTRYFNSLYGNKELASTEGFKKNVKSYNQTTTTTKSRSATNTKQESRSTEKLDKRIVFNQLDDKTLQILSSNKDNDGLVPELNKFGTGQAFSRLGLVSPLVKALRQEFTISIPSSIQQLAIPEILKSHKDVLFCSQTGTGKTLAYLLPIIQNLKKQDILDYQDSLKDEQAKLKDKEIKKSMKNKELLEDLESNKELDKQNEDNEQEEEEEDFDNIKEEEEEVNELTNKKELPGRPKAIILLPTRELAHQVMKVAKKLSHEGRFSCTAVCSGAGDFPKLRKTYKNEAIDLLVTTPGTLNKLIKSKKIFFGQLKYIVVDEADSMFTSGKGFNEELPSILEPIKYRLQHKDEPGLKPIHGIVCSATLTDQLVNSINGLFPNITKLSTKAIHKSVNSLEQRFIKVTGGGDKHDSLRKAINYKPNAKTLIFCNNPASCRSTQYFLEENGFKATSMHAELPLVRRAKHWKSFLQGDSQFLVCTDIISRGIDIGNVEHVVIFDFPSNPIDYLHRIGRTARAGNKGLVTCLIVKKDEVLAHAIQNSIQKGFTLESLSSNKRLNKNLNHHQSDLNHYLHFVLIIDVAGESCDSKVVGIAYELVLINNKKMVDFKPKWIQVDRKGVDLKILID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR82</accession>
    <name>A0A8J4PR82_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000445">
        <property type="protein sequence ID" value="KAF2070789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR82"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="116" mass="13852" checksum="9A4C60814DD64305" modified="2022-05-25" version="1" fragment="single">MGVRNKKRKNQSKEDEEDQYYNPPFKQEEFDDDVESIEDQIKREVPNTNNPAENLRKRLENKNNHFRWVVTLAGRAKRLSLHIKKTKTSTPEQYLIMAKLFEHIHLHLTEALSYSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRC9</accession>
    <name>A0A8J4PRC9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Membrane bound O-acyl transferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_006063</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000263">
        <property type="protein sequence ID" value="KAF2072625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRC9"/>
    <dbReference type="OrthoDB" id="420606at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051085">
        <property type="entry name" value="MB_O-acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13285">
        <property type="entry name" value="ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13285:SF18">
        <property type="entry name" value="PROTEIN-CYSTEINE N-PALMITOYLTRANSFERASE RASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="362"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="431"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="454"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="488"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="531"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2072625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="565" mass="66721" checksum="B79B68F610B8E084" modified="2022-05-25" version="1">MTSSPPSIGQNNTRRVNTRVHDDEDNSRNDEKKVVYVEDPATPSRWLSVEFIIYYIFIVIGYYMCIRKILNRSNEYNDGLKENDGIVDGWFMGRKQDLSDTQYRIYRENFPLLSIGLSIFVLFSNFIRRNFPDKPYSRILFYNFVSIGFLIFVHGSASLIVLGIALINFTISRMFAKSKLLPIVTWVFNGFILWTSYIYSGYKFSFIESLGEWGPYLDSNKGFLSWETYFKVSMLRFISYNNDYHWMKIKKPVMDLKGKNAYFVAQEKHQPADHYSLAHFLAYIFYIPLYIAGPIASFNAFVAQVYSPQKSMSLAEIIKASVKILLVFLGLEIFLHYCYYHSFDKSDTWMSFRGDEVALTGYVVLNFMYVKFLIIWRIFRLFALFDGIDTPENMNRCVNNNYTFTGFWRSWHGSFNKWTLRYLYIPLGGKKTQFISIWIIFFFIGLWHDLWWSWVAWALLNCVFFSIEIGIMFYFYSPKRLSLRKRWFWRYIVACAGTFNIFLLMVANLAILHGFTNSITFVKNAFFSEGGAIAFIFAYCWLYCGIQVMIEIRESEKRSKEVKPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRD3</accession>
    <name>A0A8J4PRD3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amine oxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000354">
        <property type="protein sequence ID" value="KAF2071646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRD3"/>
    <dbReference type="OrthoDB" id="16089at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF418">
        <property type="entry name" value="AMINE OXIDASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="1">
        <location>
            <begin position="198"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="463" mass="51843" checksum="4C29C144CF8D208E" modified="2022-05-25" version="1">MVSYDIIVIGSGAAGLYCAHQLNKEGYKVVVVEADQRIGGRTRQDVDFTQWPIELGGEMIHGGDTLYYQLAKENNWGIFEVFSMELFSQNSKNTFFYLGRERKLISQDQVDDDMSMLFKALGSMKEDTQGMTQEQIGEMNLLELLVKKGVPYRVLGLADAVYSKTWGTNLDRIGIREAIREDEKPQAILKNYKIQQSSKALVDFLANGLEIKTNWRAKSVDYRDGGGVSVRSYNGKTITAKAAVVTVPLTILKENDINFVPALPQRKLDAINVIGMDAGMKIIAKFNKKFWRQDCQLVLCGDSPVPQIWMDGPPLRPLDKQKPAEFVVTGFITGDQAKSIASLSPAAQIKAFLNQLDSMFGNSQDWTPATNCFISHIVYDWCKNPFVRGAYSFPSIIPSSFKYKESPNQILSEFVDKKIFFAGEATATQYELSTINGALQTGLRAYHEIKENLPLTSIRDSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS53</accession>
    <name>A0A8J4PS53_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_005919</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000249">
        <property type="protein sequence ID" value="KAF2072773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS53"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000016">
        <property type="entry name" value="Solute carrier family 25 member 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="17"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="54"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="113"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="214"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="308"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2072773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="409" mass="45958" checksum="BD0E809ED0FBA757" modified="2022-05-25" version="1">MSANSNNSNSTSDKQKVKKEKLQELFEKFDEDKDGVLNKSELKKGFKVHANIDIQDEQITKMMDIADKNKNHMIEWEEFYNILGDSESHEVGDIAEYWLQYSSKPIVFVPVDVPSWKLLLSGGVAGAVSRTCTSPLERLKILNQVQHMKLENNAPQYSGVVRSLITMFKTEGFLGFFKGNGTNVIRIAPYSAIQFLSYEKYKKAILQSTNKTHLSTYENLAVGGSAGVTSLLFTYPLDLIRSRLTVQVFASKYNGINDAFRTIVAEEGVRGLYKGLFASALGVAPYVAINFTTYENLKKYFIQEKHSPTVFQSLAFGAISGATAQTLTYPIDLIRRRLQVQGIGGKEAYYNGTFDAFRKIIRDEGVRGLYNGMIPCYLKVIPAISISFCVYEVMKKILRIDSNKISYST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS72</accession>
    <name>A0A8J4PS72_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000370">
        <property type="protein sequence ID" value="KAF2071479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS72"/>
    <dbReference type="OrthoDB" id="24303at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="532" mass="60913" checksum="2BD1D7BAE9BF47D6" modified="2022-05-25" version="1">MIDDLFFSIWRNKYLRKKIAPLSYTQYVIIKNITVLDKKYEYLSLLNHYHVPVVYHIRDLDHLCIYTSHKHNYLITHVIVSASFYKNHLHLLQQQQSPATPILFGISISESDSDSKIIGKELPLSIQYYSNTRLKPLTKDDIPRSVTSLFLGTSNIELGVIPEGVKTLVIDKQGEQIFKFENLPRSLTSLYFFSNTYSNGEKVDFDLLPPNLLSFNLNILDDLDCTGNLPKSLTCLSHNPKIIPDGLVRLNMKSITHLSDYIFPKSLTYLKYNYILFDSQSSKISFPPNLKTLLLDSCPNSILNQDNCPSGILSYSNINYFENFEVPYIPPTATKVYLKFLNEIKELPIIPTSVTHLEIVSKFAKVVPNGYFSNNITKLAFHSDQDLYPGLIPSSVTKLTLTYAKPIQAHVIPSSVKKLSLKGPKVSIELPNSITSLSFENQNQVYSDIVIPNSVTKLKVRNMDFKTFPFHLFKNVTTLSTIQILSNAKGTFEKLFSTTNIKIVKRGDFYYHCINQQLYKEVCHLKLFYLRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS81</accession>
    <name>A0A8J4PS81_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007189</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000369">
        <property type="protein sequence ID" value="KAF2071494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS81"/>
    <dbReference type="OrthoDB" id="4763727at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021243">
        <property type="entry name" value="DUF2804"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35868:SF1">
        <property type="entry name" value="DUF2804 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35868">
        <property type="entry name" value="DUF2804 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10974">
        <property type="entry name" value="DUF2804"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="267" mass="30522" checksum="435BC1E2AA4621E2" modified="2022-05-25" version="1">MYELLYIDLTSESKTDFWAYDSEYYDEIRYTPFIRETIHQSGDFANLNKQGFHLNISIDNNNNGIIKHILDVENLKLKLKIHAILNLSPSRESIVLVSQINNQLYYSCNTNTIPISGNILIGGKHFDASKCFGVMRFFRGITNSVKYIVWFTGNGRINGKPLGINIGQGLGNVDSLTENGVCVDGIIHKLGQVEIEKVSEFIWYLKNKRFGNEGKLMFTLLRKVEQENHFIKFTRVVGKFNGRFILDSGIEINILNLVGYMHISKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSN9</accession>
    <name>A0A8J4PSN9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_009937</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ELP4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000859">
        <property type="protein sequence ID" value="KAF2068741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSN9"/>
    <dbReference type="OrthoDB" id="289162at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19494">
        <property type="entry name" value="Elp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003211">
        <property type="entry name" value="Elongator complex protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008728">
        <property type="entry name" value="Elongator_complex_protein_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12896:SF1">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12896">
        <property type="entry name" value="PAX6 NEIGHBOR PROTEIN PAXNEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05625">
        <property type="entry name" value="PAXNEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="374"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="402"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2068741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="424" mass="46910" checksum="48A8B7D484011B22" modified="2022-05-25" version="1">MTTPLRPPTTFVRKVAGKPAGAGAPGASSTTTTTTQAKKLPNGCKISIQNGNLLTSTGLQDLDDILGGGIPVGSILMIEEDINSSYYMFLLKYFLAEGILQKQGVFFSSLIGLDPFEVLNKLPARITKEEEIEQDKQDVDSNATTKNPQDELKIAWRYQQYVSNEMSKQQHQQQQQASLQQNTFCHSYDFTRKMNVQSMIPEQIHTLSYSSTSQVEGSSPYRNLFLEIQNLVYKYNQEAASHPENTKVLRLALQSFSSPLWSNDEEGVIEFLHALKGLLRSSVSTCLITVPTYIYNSAFVKKISHLCDTVVSINSFSGLGGDTPEQFAEYLGLFNIKKIARLNTLSLSFHPDMLTYVFKMKRRKMCIETIHLPPEMSRSGDSKPDSSSSNKKSEDIVSKMKSGSGLLCGASSGGPAKSNSSLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSX2</accession>
    <name>A0A8J4PSX2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Molybdenum cofactor biosynthesis protein 1</fullName>
            <ecNumber evidence="6">4.1.99.22</ecNumber>
            <ecNumber evidence="7">4.6.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">CYY_004713</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Isoform MOCS1A and isoform MOCS1B probably form a complex that catalyzes the conversion of 5'-GTP to cyclic pyranopterin monophosphate (cPMP). MOCS1A catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate and MOCS1B catalyzes the subsequent conversion of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate to cPMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate = cyclic pyranopterin phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:49580"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59648"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.6.1.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>GTP + AH2 + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + A + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49576"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.1.99.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Isoform MOCS1A and isoform MOCS1B probably form a heterooligomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the MoaC family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the radical SAM superfamily. MoaA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.22" evidence="6"/>
    <dbReference type="EC" id="4.6.1.17" evidence="7"/>
    <dbReference type="EMBL" id="AJWJ01000172">
        <property type="protein sequence ID" value="KAF2073968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSX2"/>
    <dbReference type="OrthoDB" id="429626at2759"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061798">
        <property type="term" value="F:GTP 3',8'-cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21117">
        <property type="entry name" value="Twitch_MoaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000117">
        <property type="entry name" value="molybdenum cofactor biosynthesis protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.640">
        <property type="entry name" value="Molybdopterin cofactor biosynthesis C (MoaC) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01225_B">
        <property type="entry name" value="MoaA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013483">
        <property type="entry name" value="MoaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040064">
        <property type="entry name" value="MoaA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010505">
        <property type="entry name" value="MoaA_twitch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023045">
        <property type="entry name" value="MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036522">
        <property type="entry name" value="MoaC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050105">
        <property type="entry name" value="MoCo_biosynth_MoaA/MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002820">
        <property type="entry name" value="Mopterin_CF_biosynth-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02666">
        <property type="entry name" value="moaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00581">
        <property type="entry name" value="moaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001199">
        <property type="entry name" value="PRK00164.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006870">
        <property type="entry name" value="PRK09364.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960:SF0">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SYNTHESIS PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01967">
        <property type="entry name" value="MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06463">
        <property type="entry name" value="Mob_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01383">
        <property type="entry name" value="cyclic_pyranopterin_phosphate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01386">
        <property type="entry name" value="main_SPASM_domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55040">
        <property type="entry name" value="Molybdenum cofactor biosynthesis protein C, MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01305">
        <property type="entry name" value="MOAA_NIFB_PQQE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="15" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="17" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="21">
        <location>
            <begin position="80"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00015273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00054222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00063038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAF2073968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="607" mass="66974" checksum="EC4766290FC547A5" modified="2022-05-25" version="1">MLRAYTNKLISGCGGSGVVNSLLYGNAINGSFIFTKNQQQQQQQQLSQYRYFTSTTDKPASPTSATPTTPLDKNNILTDTFNRKHTYLRISLTEKCNLRCQYCMPEEGVELAPNDKILTDDEILRISRLFASAGVTKIRFTGGEPLIRKNIESLIEQVGKIEGIEKIGITTNGLVLHRKLERLQKGGVNLLNISLDTLDTHKFTLITRRLGWEKVMESINKALSLNFNPVKINCVVMKNINDMEICDFVEMTREKNVEIRFIEYMPFGGNKWSDKKFVSYTDMIKMIKEKFGALDRCVDEANNTSKTYQVPGFKGRVGFITSMSEHFCSSCNRLRMTADGNLKVCLFGNTEVSLRDIMRSGASDEEILKVINQAVLKKKASHAVVEGLVLCFVNLSNTITSKLGVATAAMSSALFPSVTPNPLPSMRSYSTFNSGGVHNNNNNNNNNPFSHVNSTSNMPTMVDVSAKVPTKRTAHARTIVEFPTDVLQQLLGSDRKEISSKKGPVFATSIVAGTMAVKNTSNMIPFCHPLGIEAIKIDIAVISDAQVQVDCTVSVFSKTGVEMEALVGASLTSLCIYDMCKALSHDIVLKETKLISKKGGKSDVGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSY4</accession>
    <name>A0A8J4PSY4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin-dependent kinases regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_006260</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the CKS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000281">
        <property type="protein sequence ID" value="KAF2072425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSY4"/>
    <dbReference type="OrthoDB" id="440676at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.170.10:FF:000001">
        <property type="entry name" value="Cyclin-dependent kinases regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.170.10">
        <property type="entry name" value="Cyclin-dependent kinase, regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000789">
        <property type="entry name" value="Cyclin-dep_kinase_reg-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036858">
        <property type="entry name" value="Cyclin-dep_kinase_reg-sub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23415">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT/60S RIBOSOME SUBUNIT BIOGENESIS PROTEIN NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01111">
        <property type="entry name" value="CKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00296">
        <property type="entry name" value="CYCLINKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01084">
        <property type="entry name" value="CKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55637">
        <property type="entry name" value="Cell cycle regulatory proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00945">
        <property type="entry name" value="CKS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 4" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU311113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2072425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="82" mass="9729" checksum="9EC68F2906996B9E" modified="2022-05-25" version="1">MPATIHYSLKYYDSQFEYRHVILPAEISSLIPRGKLLTEEECRKLGIRQSDGWEHYANHKPEPHILLFRRPHNGIPNEVDYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT04</accession>
    <name>A0A8J4PT04_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_004690</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000170">
        <property type="protein sequence ID" value="KAF2074013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT04"/>
    <dbReference type="OrthoDB" id="276546at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02932">
        <property type="entry name" value="OYE_YqiM_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044152">
        <property type="entry name" value="YqjM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43303">
        <property type="entry name" value="NADPH DEHYDROGENASE C23G7.10C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43303:SF4">
        <property type="entry name" value="NADPH DEHYDROGENASE C23G7.10C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="6">
        <location>
            <begin position="38"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2074013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="404" mass="44864" checksum="F2A20C66C560B9A4" modified="2022-05-25" version="1">MDSEPKIQEYHPELLFNLPKNYGSVGKPVKGVSNVSMLFSELRIKDLTLKNRIVCSPMCMYSCEDGLMNDFHLVHLGSFAKGGCGLIVFEATAVQSEGRITYADSGLWNDAQIGPLKRINDFVHKFDSKTCLQIAHAGRKASTYPPFLGMRNVTIPADDIKNRGWDIKGPSAVPWGEESLVPAEMSIDDIQETIEAFKATTLRAMEAGFDAIEIHGAHGYLISSFLSPTSNKRTDRYGGSFEGRAQFLVDIVRAVRSVLPSSVPLFVRLSCEEWVKDGIHIEDTVRLAKILETMDVDLLDCSSGGNSSNQKIAGGPLYQVPFAERIKSSTRFLTGAVGLINKPNEAEKILREDKCDLVFLARGLLRDPFWAIHAAYELEVEVDNCLQYSFVDKRYMQGLRSSIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT52</accession>
    <name>A0A8J4PT52_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006147</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000271">
        <property type="protein sequence ID" value="KAF2072530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT52"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="540" mass="62051" checksum="572BD1374863EC2E" modified="2022-05-25" version="1">MDTLFYSVWRNTILRKDIRKKVCQNLLIDINLEYLNDNHQYISLLSLQDKIDYNIYFKLFIKEGEELKEYYNNPHKHLVDDIKIHHFNRNKNVPPEENIFDADIICYGVRRLLLHEDHVTNVRGRLPPTITELYFNGDNSKLTYNLRFGNKELDAMISSLPNLRKLVLPNNYQVTDKIELPSTLVEFKHQSSCDNLRKITVTPTTPIQLKGCEVNVEDLDDLAWFRDKPWYTNLEIIADGTITRGAIPQHVTKLTFPFYQETRVEEGAIPAGLEELDCPLTIPLTIIKGLGHLKILNLHSASQPLEKDLLPKSLESFSIYKDNLPFLPDVLPQGLKNLQSTHFDLDLQVGSLPHSLKKLEFSTFDQKLDPFVLPSSLTWLNMFRFTGKLVKNSLPSSLQVLKLNSFTGSFEFVEPLPELFVLQVDTLQPSVTNLFSKHNINSEKRVKITFKTIGDQVSLQDLPIQHLHLYNGHMRSTLSAKLVPKRIKSLRLNGLDIKSSGLIPNSCIYLEASNKDAVNFIPSTTKHTLYNPIKYAHQIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT54</accession>
    <name>A0A8J4PT54_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Polyketide synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_005318</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pantetheine 4'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:47942"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000208">
        <property type="protein sequence ID" value="KAF2073373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05195">
        <property type="entry name" value="enoyl_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00833">
        <property type="entry name" value="PKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.110">
        <property type="entry name" value="Polyketide synthase dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013217">
        <property type="entry name" value="Methyltransf_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032821">
        <property type="entry name" value="PKS_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042104">
        <property type="entry name" value="PKS_dehydratase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049551">
        <property type="entry name" value="PKS_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049552">
        <property type="entry name" value="PKS_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013968">
        <property type="entry name" value="PKS_KR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049900">
        <property type="entry name" value="PKS_mFAS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050444">
        <property type="entry name" value="Polyketide_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45681:SF6">
        <property type="entry name" value="POLYKETIDE SYNTHASE 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45681">
        <property type="entry name" value="POLYKETIDE SYNTHASE 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23297">
        <property type="entry name" value="ACP_SdgA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13602">
        <property type="entry name" value="ADH_zinc_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16197">
        <property type="entry name" value="KAsynt_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08659">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08242">
        <property type="entry name" value="Methyltransf_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21089">
        <property type="entry name" value="PKS_DH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14765">
        <property type="entry name" value="PS-DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="Probable ACP-binding domain of malonyl-CoA ACP transacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52019">
        <property type="entry name" value="PKS_MFAS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="7">
        <location>
            <begin position="4"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="PKS/mFAS DH" evidence="8">
        <location>
            <begin position="915"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="6">
        <location>
            <begin position="2424"/>
            <end position="2502"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal hotdog fold" evidence="5">
        <location>
            <begin position="915"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal hotdog fold" evidence="5">
        <location>
            <begin position="1051"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for dehydratase activity" evidence="5">
        <location>
            <position position="950"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for dehydratase activity" evidence="5">
        <location>
            <position position="1113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS52019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2073373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2921" mass="332300" checksum="B062FA2CA3CBC4C5" modified="2022-05-25" version="1">MYRRNRVAIVGLGLRLPKSKSPQEFWCNLVNKFDGISQVSKERFSSNFYEIGYWSSNRAGFLELDEWLSFDPLFFGISPSDAAGIDPQQRLLLKVVYEALQDAHIDPQQVRGTNTSVYIGCCSTDYKGQLLNYEKPSLSQETSIYAIANRISHTFDFRGPSMTIDTACSSSMSAFSLGFESIERGDSDLSVVGGVGLLFNSEYFSYLSGLNALSPDGECKSFDASANGFARAEGSTILVLKNLDRAIQDGDTIYSVVEGVNSNSDGNFEKGNFHSPSASAQSENLYRALSKGNINPSDVYYFECHGTGTIVGDKVETSALSKVFKDNHSQDQPLLIGSVKSNIGHTEGNSGACGIAKVCLMMKNKTFVPNIHFNTPNPNIDFDNWNLKVVTECTPFPLIDRKVVMGINSFGITGSNGCVFLSEYKAPTSVTQKNINETTTNSNNNLLVPISSNSKQSMDKYLTTIMDNDFISDVKDKLEFNDFIRYHILSKPTQYIQRSVLSCTIQDGWNNFSESKVFRNEKETTSNMFSIQSNPKVVFVYCGQGPQWNQMGLNLYEKEPTFKKTVDLIDDKLGKHFGYSVWNKLLQTTDELSIHDPLLAQPSMFILQCALTELYKQWRVIPDIVTGHSFGEITSLYYSGIISLDSACKIVYERARLQQKTVGSGRMLALSLSKDDFDKRFKSMYPDIEIACFNSNNSNVISGSEANLLSIKSILDKDNIFCAFLGTRSSFHSSSQESIKNEFFDSIDNNLYNNNNSGSIMFSTTTSNQITKFSTQLLYDNLRNPVLFSQTIHNISNFIEDNTKTIFIEISPHPTLSRFIKEIYDQLDKKNITIINPLNKKSKDQEQLKFNETISNLYINGYDIDFSCQYTCEQLLDTTFKSKCQHLIPPYQWDDEIYYKESDSMKRLRLVGPSIDCLGNPVLITQIQNSKAFKTVINTDRNPFSYLKGHMVDSRNYFPGCGYIDNIMKAFTDSDHVIISRLEFKQPLILKPSTDYELHTTITQVSKNEHKAEFHFSANNKWINSATGKVIISPPFTNRKLDFSSLQSKCNLSTVYKTDIYQYISATTGLTYVDQFQRVEEANLGDGCSLSKISIKPRLSNNDTTLFNPAILDSCLHGIFPLAKGPLNILFDRISNMKIYTENIKNIKIDDIDFIYAFTDNLQIDKIDNRITSSLKVMLDDGTLLFEILQLTCLSLTPIKSDTIKYPNKELYSLFWQSIESLPMHKWIADDLNSLEPVNFLDNLNYQKYCSLLFYNHIKKKAPSSLDKSVIQSTSINDLINHYLLDTNIKSKYSNLFRLTFKVLQENPEYMVDDESELNRILESIVFKDIYINYTGVLKKSIPVIASLLFPSPNLEIEDTQKYVFDDDEANNLYLNHYLSENIRVLCNNICKLIKENIKEKRVVRIIELGSGTGSLTSDILKSLHQLLIQNEDHCQIQIEFTYSDISSSFISQAKSLFGQYSDKINIIYTTVDIESDFLEKQQLKGSYYDFIVLANVMHFASQASFPLQEIYKILSYGGHLLFLETITGLTHMDFYYGAVDQWTGYKDTDIRSDHYCMNPEQWKILLEQIGYTNICSTTSNHLHFVMYGQKPLINDKLSLLKESTIVKRKVIIFQRFTDENSGTIIDKLSKHCQQTIVIANHLELKRFISEIHDDDIIINLSILSEIDKNSLNQVTMEFIEINQEILKKKSRCKHILLSLNSLMDSKYYLNASVVGASRYFNRFNDCVLIDFDQQTLQHPSFNIVNMIELLMDSKSNNYKEFAVRNCIPHIELAGKELIRDSPSYENNDFYATFKLNFSLKLSTKLKELSPYQVEVEIKASGINFRDNLVYRDLVSQQFTLFNGEKHFVGFGGEMTALVTRIGEKVSKVKVGDRVCGIVNSFTNKAMVNEDELCILPDSISYTEGATIPVVYLTVYYSILRLGRLFDDYDKDESTKSILIHSATGGVGLSALNLLKWKMKQTGIKVYIFVTVGTKEKQDIIKEKYGSMVTGIFSSLNTDFVDDIKNQLLELGSTRGGVDVILNTTASEFMHSNFSTLAQDGKMVDLSVTHLYNNEFLDFNNFRHNISYITTDVSSYTRSFQIKVFNEIVDAISKKELDLIPVTVFPIDKLKDALEFIGERKHIGKLVVEYDKDIVTPDVIKAYKNKVLKNDYQIQTTSKCVIITGQQGLVLETLNWLISNSNSITDVIILTRSTMKWEIRKTINQYKSKINFHFRSIDIGNYEVMESTFKKLYNNNPQLPPVDCILHFAYLHTESEPLDITLDEITAVHNAKTMGLLNLHELSLLLKWDIKHFIISSSIIVLNPTYNQCAYISANLVYDSFSKYRRSLGLECSCLIFGPLEGAGYVSRKKSVENHLSHIGYHPLPLAKTLGSIDLAFSQTYSKSNLITGIFNFSIIKKNGHIFPHFEYYTNHIQSTSSAYAGETSLYSIRDKVINTIADLLSISPSKLNSSMKFKDYGVDSLFMVQLKNWIEKEFNKPNIISVSQLQNSTIDSIIERIQKNIDNNNSSKNKITIVDQKDQPFLSDLFIETHPMDYWQNEIKLDEKIRSDPNCKLESNQGRILLTGATGFLGSYLLTDLLHQKDCKIIYCPIRANSTEHAKSRIIESLSKYKLLHLLNENDLENRIIPIVSDLNQESLGLNQNQFNNLAENIDIIIHCAATVHFNVFYSDIKDKFMLKELLKLIASNQNRLIRFLELSSTSVYLSTNNNNNNHNSGVNENKIPSLDEQNTKFGYAQSKVVQEYILNEAYQRGIPCVITRPPFIFPTDVPSYDSFQLFFFSCLKVKKCPILVDSKAHVESLKVISSSICKIAMNDKYWIKSLNHADQVPQLNLISEPIQISSVIDTISKTYSYPMIDFEEWKQLVAKSNDPICKKIVPFLPGLIPAHMKKFVKGKGNQQEELVSAKDHLSTESIFHIIEKNNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTA0</accession>
    <name>A0A8J4PTA0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptide chain release factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_006058</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000263">
        <property type="protein sequence ID" value="KAF2072620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTA0"/>
    <dbReference type="OrthoDB" id="2019491at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000004">
        <property type="entry name" value="Peptide chain release factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.410">
        <property type="entry name" value="Release factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43116:SF3">
        <property type="entry name" value="CLASS I PEPTIDE CHAIN RELEASE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43116">
        <property type="entry name" value="PEPTIDE CHAIN RELEASE FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="Release factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptide chain release factor" evidence="4">
        <location>
            <begin position="152"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="405"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="406"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2072620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="442" mass="49650" checksum="9107E2D673D7D079" modified="2022-05-25" version="1">MISRLISKSLSSLSPYQSCSLSSTLFVKRLSKQIKYINSDSDQQFYQQFNPLCYTTTTTTNDNNEISNINSTQEIQFKEKEISRILDLIENSQQFKNNVAQLKRLKSTLDLEGGMIVGNDPSVVKVLKQHNALKADVEEVNKLVNGFKDYRELADMLRRESDNEMESEVNSIVNNLYHQSTELELRSLMNGANDRESAYLEVHAGTGGTDSMDWSEILINMYIKWSQRKGFNATLVDVSMGELAGYKKAIVKIEGPFVYGWLRTEMGVHKLIRISPFHGSGAKSDKRHTSFTSVVVYPMADDSINIEILPKDITIETFKSGGAGGQHVNKTESAVRITHIPTGLVASISDQRSQHQNKQIALDLLKSKLIAREIKKKAEIEKAFRSEMGTNGFGSESIVRTYTMHPTERVKDSRTNSETSQLSSVLDGEDELDQMIKKALAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTC7</accession>
    <name>A0A8J4PTC7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005732</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000236">
        <property type="protein sequence ID" value="KAF2072966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTC7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="568"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="124"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="295"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="348"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="499"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="511"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="594" mass="66985" checksum="E35A1222AA845964" modified="2022-05-25" version="1">MATRRRTTKAEVEEKKEDKSTILASLSKTSRRGATIDMKSLLKIAEPSPAKKKLPVRRKTAAAEVEAEKAIETTTKEETTPEPVVISTPTKAVAKATKITTPKPEKTVSKISTPNTRSTRVAYPTTPTNTNTNNNDDSSDSSEDEPPKPPARVLKRRVITPPTPAPVPTPTPTPVPVPTTTSEVEKTPVQPRKKVKPDTPFPDMKNMNNSKSQPPSSFEFRVPDVPLTKLFKEASTVDNTTTTSTKETTKALEKTTTPTKESPVKVVKEKTPVKPETPVKAKTPIKQSPAKTIKETTPIKTTTPIKESPAKVVKQTTPVKTTTPIKESPAKVVKETTPVKVSKQTPIKTTTPVKSTPVKSKQSTPVKLVEEEEAEDEDEEMNELDSQDDDNSNEDEGEDIMEQDDDDEEDEDIENGEDDEEEIENEQASNESQEEQEEEEVEEDDDDEDEKEDEEEEEEEEKDNDTLEELDPSLLKAVYSKQDQLKKEQIKQQTLNSQLEKEKQEKEKRDRIKKKREERRKRATPSFMKVISIDNVDEYTHYLKKQYKEKEEADKKELEKRLYESANQKAKNSIAHKNLRFGSRQKVSKRSLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTJ3</accession>
    <name>A0A8J4PTJ3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vps72/YL1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_005499</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000221">
        <property type="protein sequence ID" value="KAF2073182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTJ3"/>
    <dbReference type="OrthoDB" id="49520at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029525">
        <property type="entry name" value="INO80C/Ies6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013272">
        <property type="entry name" value="Vps72/YL1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31200">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31200:SF1">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08265">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00993">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Vps72/YL1 C-terminal" evidence="6">
        <location>
            <begin position="234"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="28"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="74"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2073182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="285" mass="31469" checksum="D46C4444841BD302" modified="2022-05-25" version="1">MVTHKRLRKQTQVLLSDSEEESNDSGVDDPVVTTSNRVGSRNSSNSNNNNKKGMLSDSEEDSNDSASASGSADESNEDEDVEIDLEQDSEQSESEDDMSDIEEKRTNKKKKVTNTKSQPVAAAPPAQRGRKPKSASTSPPLPSPPAPAAPKKRPGRKKLEQSPLAADGSNSAEITVNIVKPFKNLNFSFPIESVKKPWKSVKQILASENTSNLKATFPSYQNIEVSSTLIPTKKYCDITGYIANYVDPKSQLRYYDSSVYKLIQTFPDNLQQSYLNLRRGNTGYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTP2</accession>
    <name>A0A8J4PTP2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005215</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000202">
        <property type="protein sequence ID" value="KAF2073465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTP2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="733" mass="87066" checksum="E4989396C4A5D2E9" modified="2022-05-25" version="1">MNDQQYTFLSVYRNTFLRTHIFSFLLVSRKGYYVFHDNKLSLNYHQLNTLDIIKLNNNHLLKEKLNRFKSVINNSNSNSNSFTCYQDWLDNKNYEILNYVILQYESDSWQDDDDLLATLIDLFVESKKSTFILTCFKTFGITRSRFQKFAKYIPKRVHYRKIRDKELFIELVNDDRIQIDHYYLLLNDQLFIENKDIFRTIKDWSDEKFLCDLLVQGLENQHKISLLCWILTVSPLYNYDRYMEMSAISTTTTPRVDYVYLYGIKIIHNKDHPFYHGSNSDMKSYQAAFCSQEIMEYTIKHYPDAFATSFSEEYFKRIIQDPVKSKLLIEYNNNTNYPYLFDPTSDDMEIVELCHKYGQIVRESILITSSEKVQCISERLPNLFHDVFHQCLIYACSTNNSECMKYCLDNVPGDFNVNNTLKDVFKAGGNNDAFKCFYHWVIVNINTKYLDGVGSDMQSKEDIFHFLCCLYSSKEHQTFDIFKSYYEKIKSPNLFVDQLLKNAYLDLKSCQKHDKQESIKILEYVKNQYQIHNSPPADIDYFSVFYQDRDYSQLINYKKPQNIYSAIKYAFVWGSVAKMEKILDLMDQEQFSDFVQQVQDIVLSHFYFNPPSQVYCIQFKVALYCFERFADQFSQSTLDSLCHIALITDNHKLMDILLHKTKVRLIRPQSPEHDKQMLELNYMNLTKSENSSRTNYFTLHPFTLDIDDQTLNNHIVPPFVEPKRSPPSPHFIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PV17</accession>
    <name>A0A8J4PV17_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005461</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000218">
        <property type="protein sequence ID" value="KAF2073234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PV17"/>
    <dbReference type="OrthoDB" id="21388at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="298"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="386"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="171"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="308"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="408"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="430" mass="47175" checksum="598F6EDE7286F254" modified="2022-05-25" version="1">MPLMKLGDKRKKNRRNRQNKKKKRDQLNESVDENSFIQDHILVNSQNNRNVSPKKIGGVNVMITQCYNILQNSFSLSSFVNRFDSAFSYFKTLLKNEKGDKMVERDNGGVIVNGKELTTSQLLSSPMVPSIIIQQQHHHHNSFGSSSTNSNNSSLSMSSMSESSLNDQLKIYQLENKISQMEKEIQRMRNEQTQTHKQNLNQYHELLKQIINAASLIQSQPPVIINQAPVFAPVCAPPPPPPPPPPLMMNKSTAPVIKATSPISRNPIAPKKPAASTPQFSISMADLSSIKLRKTSSKLLGANSPSRAANKSPAKSPLSKKAVSPMKKLLNTPLKGTTRPSISSPLKISSSINNIASPNSIGSIKSSSLTTSSFAAVPKSPLVLKDVTNSTNNSNNMTKSPLLMKKPNTPSKSTTFESPFKNPLTISSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVB1</accession>
    <name>A0A8J4PVB1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004408</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000155">
        <property type="protein sequence ID" value="KAF2074275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVB1"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="43598" checksum="E9476B4729AE27CC" modified="2022-05-25" version="1">MLSKRQNVKLQILREDHEYVSQFINEFLEKDRSFIKEMYIDMYNELTPNFIHQGVEHVHFGDYLRTSLDCSLIPSSVKRLNIEKCYNFDFILTPTTLHNTLVELNLFKAKEPFPPGLLPKSLTKFTIKSYDHTLVNGVLPCALKYLGMNNLKNCEPNFKFPDSIQTLSTMCSVVMDHIPQNVKNLLLKGDVCISRPLPKTLSTMEYSVFSTSYDFTNWLFNTQLTSLSLSITNCYPIDIKQGMFPESLLQLSIHQFRGNIKANGLPSSLRELDLSFHMDQKEIKSLYLPNSLKKLRLNFSFKIHFKNSLVLPDNLKDLEIESDNLDLLKGCSFPASLEKLKVPYKILPKKITMPNLLSVKYYIEPNTILKKPIFQHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVC3</accession>
    <name>A0A8J4PVC3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UbiA prenyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_008369</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000509">
        <property type="protein sequence ID" value="KAF2070316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVC3"/>
    <dbReference type="OrthoDB" id="203513at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042371">
        <property type="term" value="P:vitamin K biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13962">
        <property type="entry name" value="PT_UbiA_UBIAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026046">
        <property type="entry name" value="UBIAD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929">
        <property type="entry name" value="1,4-DIHYDROXY-2-NAPHTHOATE OCTAPRENYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929:SF0">
        <property type="entry name" value="UBIA PRENYLTRANSFERASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="271"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2070316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="292" mass="33224" checksum="2F370BEDB000BEC7" modified="2022-05-25" version="1">MISLKIAGIIHGFRLKAILMSFTTITIGVVFSYQETGIISPKLWAHYLILMMIYTYATILNSYYDWLLGVDDEKTTTNRMMFDYGITKTDVQNYMIFGIVVECLLFHYCFSHLAWLDYCVALFIIVQISLISVFYNAPPLRMKNILFGAEIAVFGSYGLLTFAAYFFNTGIMTFNSFVYGIPCFILGTLTITGNLLHDIEDDRKGGSYSSAMHFGPIGSYYIFISWIYIAYISLIIISIIKSNMLINLPFLIVPYFSFISKRVKNQNYQNIKLLGLSAVSLFNILYLVGGCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVE5</accession>
    <name>A0A8J4PVE5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_005244</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine 5'-phosphosulfate + ATP = 3'-phosphoadenylyl sulfate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24152"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homohexamer. Dimer of trimers.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000203">
        <property type="protein sequence ID" value="KAF2073459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVE5"/>
    <dbReference type="OrthoDB" id="468at2759"/>
    <dbReference type="UniPathway" id="UPA00097"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004020">
        <property type="term" value="F:adenylylsulfate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004781">
        <property type="term" value="F:sulfate adenylyltransferase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010134">
        <property type="term" value="P:sulfate assimilation via adenylyl sulfate reduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019379">
        <property type="term" value="P:sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02027">
        <property type="entry name" value="APSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00517">
        <property type="entry name" value="ATPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000802">
        <property type="entry name" value="Sulfate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000052">
        <property type="entry name" value="Sulfate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.10">
        <property type="entry name" value="Sulfate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00065">
        <property type="entry name" value="Adenylyl_sulf_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002891">
        <property type="entry name" value="APS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059117">
        <property type="entry name" value="APS_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025980">
        <property type="entry name" value="ATP-Sase_PUA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050512">
        <property type="entry name" value="Sulf_AdTrans/APS_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024951">
        <property type="entry name" value="Sulfurylase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002650">
        <property type="entry name" value="Sulphate_adenylyltransferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00455">
        <property type="entry name" value="apsK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003013">
        <property type="entry name" value="PRK03846.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004040">
        <property type="entry name" value="PRK05537.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00339">
        <property type="entry name" value="sopT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42700">
        <property type="entry name" value="SULFATE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42700:SF1">
        <property type="entry name" value="SULFATE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01583">
        <property type="entry name" value="APS_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01747">
        <property type="entry name" value="ATP-sulfurylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14306">
        <property type="entry name" value="PUA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-sulfurylase PUA-like" evidence="10">
        <location>
            <begin position="23"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Sulphate adenylyltransferase catalytic" evidence="9">
        <location>
            <begin position="190"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="APS kinase" evidence="8">
        <location>
            <begin position="413"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00062002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2073459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="589" mass="65630" checksum="58D95A872488E054" modified="2022-05-25" version="1">MVSFNQGESSDDAVFDYNSIGAIPHGGELVDLILVGDELDQLKKRSTHMPSLLLSKKQLCDIELLLNGGFSPLTTFMDQSTYDGVVENMKLSNGLIFPMPIVLDISQETLDIVQATESKDLALRDEEGNLIAVIRVESFYKADKIREAKLTMGSVDPYHPGVNLIFNTKEYYVSGKLEGAQLPVHYDYNDLRRTPKQVREMFREKGWKNVIAFQTRNPMHRAHRELTVRAAELNPGCNILIQPVVGMTKPGDIDYHTRVKCYKSIIKSYPDGLAALSVLPLAMRMGGPREAVWHAIIRKNFGCNHFIVGRDHAGPGEDKNGNLFYQPYEAQEMALESQSQLGLKILPFQMMVYVPAHDKYYPVDEVPAGLETANISGTKLRHLLRTGGEIPDWFTYTQIVKILRDSCPPRSKQGFTVFFTGFSGSGKSTIANALNEALLEDGSRSITLLDGDVVRTFLSSELGFSKDHRDLNIKRIGFVASEISKAGGIAICAPIAPYSEARKFARDLISNVGGFVEVHINTPIETCEKRDRKGLYAKVRAGQLKGFTGIDDPYENPENPELRIDTTDVSVKEAVALILNHLRKEGYIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVW0</accession>
    <name>A0A8J4PVW0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2 5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_004987</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2 5"/>
    <dbReference type="EMBL" id="AJWJ01000188">
        <property type="protein sequence ID" value="KAF2073699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVW0"/>
    <dbReference type="OrthoDB" id="19107at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000006">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.340">
        <property type="entry name" value="Nucleoplasmin core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041232">
        <property type="entry name" value="NPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036824">
        <property type="entry name" value="Nucleoplasmin_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023566">
        <property type="entry name" value="PPIase_Fpr3/Fpr4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43811:SF19">
        <property type="entry name" value="39 KDA FK506-BINDING NUCLEAR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43811">
        <property type="entry name" value="FKBP-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17800">
        <property type="entry name" value="NPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001473">
        <property type="entry name" value="FK506-bp_FPR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69203">
        <property type="entry name" value="Nucleoplasmin-like core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="7">
        <location>
            <begin position="257"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="92"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="154"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="93"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="188"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="201"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2073699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="343" mass="37100" checksum="5E90F5CE77C383C4" modified="2022-05-25" version="1">MFWGLEVNKSVKFQTPFDLHITTACLDASAKGTERTVVQVEYEGKTYAICSLSVNGIASATLDTNFEQGKEIAFKVSGNNTVHLTGYFVDSMLADDSDDEEDSQEESDEEINSDEIDFEEGAEDDDEDEEEEDEEDEDEQKVLAATVAAALNKRKLEINQNKEPQTKKNKIEQPAVTKAQTTAAASPAKKEQTPTKKEQTPTKVQPAAKPAASPAKKPAAVPAQSPKAEEKKGKVTLKNGLQYEDIQIGTGPTPVAGKRIAVKYIGKLQNGKTFDSSLNKPFSFRLGVGEVIKGWDLGFAGMKVGGKRRLVIPSNLGYGPQGAPPSIPGNATLIFDVELCKTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW03</accession>
    <name>A0A8J4PW03_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000137">
        <property type="protein sequence ID" value="KAF2074631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW03"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="76" mass="7928" checksum="DBD424B69060A331" modified="2022-05-25" version="1">MLFNALSSISNPMKSISNKTVNNGSSASSSIGKTGNQCWYIIGIGGATVWVDDSATPHSYHMGYPPGTFSSSANII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX80</accession>
    <name>A0A8J4PX80_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004308</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000150">
        <property type="protein sequence ID" value="KAF2074374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX80"/>
    <dbReference type="OrthoDB" id="61900at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="230" mass="26858" checksum="6CB4AB3832F786BE" modified="2022-05-25" version="1">MEMQKKECFNELLGLELKWWTLLHADHIKEIQEKGYNIENLLFFAEILFVAENLKPMMMISELSSISLNQSFIKDVIVSSRLLQLYAHMDVVLVDEIETPNTIFNNCIIIYNTLSPHYPLLQQEILCKLPTSNTTTTASHKRVSTRLVIDDEKLSLLFNYPVQLVETDDAYMIDVGYSCNGKIVFSFIASQHQWTDNQSTIQQHFDLFSQKFKEIYEIQLQLLYTKFQVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX88</accession>
    <name>A0A8J4PX88_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">MACPF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_007468</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000400">
        <property type="protein sequence ID" value="KAF2071216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX88"/>
    <dbReference type="OrthoDB" id="21110at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742">
        <property type="entry name" value="COMPLEMENT COMPONENT C6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742:SF8">
        <property type="entry name" value="FLOCCULATION PROTEIN FLO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0204">Cytolysis</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035214955" description="MACPF domain-containing protein" evidence="5">
        <location>
            <begin position="24"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="domain" description="PA14" evidence="7">
        <location>
            <begin position="299"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="MACPF" evidence="6">
        <location>
            <begin position="499"/>
            <end position="845"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2071216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1140" mass="127821" checksum="066A0005020E86FF" modified="2022-05-25" version="1">MRYIIFTIFISLFLSLTLGYTSSNPTFYLNPLSICKSDCGSEDQPFSNFKDAINYLSSLKSETPLVLLVDQGDYYGENNQQITIDFNLEIRSIYESDETVLDCQDTTFAFKALGQSTLLIKGLLIKNCVSYKGGAVYTENKSTTLQNLKFISNFAQAGAAIYSAANVLDIESCVFINNKGVSSSVEISKSITKLSKSRFFNNSGRDISCSQTSVDSSESFFTSACFACEMYDQDLSSLCNNTVVSELNDCNRDGVCDSFIETHDTCPYDCSDENTICNSNGICEPLENFKNCPSDCLADLHPGWKLEKFEYEISKPNRTYTNYPNPVDVEFLGFPSILDFMGKQYPPLSGKLSSNIAITETTNYYFQLSVSNLAAIAFIDGRVLFDNFFKENINPMVLERKLLLSSQRKHFIEIYFIVSNDFQRNLELRWKKQIDEEYTLIPSAFITLEETIECGDGICNEEPTTCLIDCHDQFEPDCSPTSPAPPLQSYYYPVQDMVGTLLNTQYLSTLPGMKYLSQGIDLETWKSKPTSIFAHTYCDDTSFSLAHFVYRDAVYTIPKGLHAQISPTCTSDSTTKVFSSSYSFAKNSAEEWGVSVSLSGGLDLKAVSFKASASFSKHESVKKAEELEKQADGSLYQSTIVCDVFKINMVEPYRWNPRFLEDIGSAYVDDKDPEQARNKTTQNLVQVVKNYGSGFYKSATLGGKLEQLSVVDNSYEKSSSSQETESNHDWSFSASVSKGIFKGSAKYHGSIENKKSSSQMQEFVKNSKRSTLTISGGAPGSYGEDEPNAFETWADSLDKLPVPIEGEVRYVADILPTNWYFKNNISVKDLWADAELLIFKDIYKQAKFKYTDPSDPKVEYLIDHLGKNQGLYVLIGKDEIEPSEGCLKEFSSLTIDTIVTTNYTPENTLVAVFIKGKKAKHILIGAEINDDQGIRAMTFRDAKGDAIKMCEDLQFISMSTTRQYNLTYDNNLSKYVSAAPVFESLHLQYENMKITPDCTFCTVEMTIIGTEGRQTDILGAVKSFSAHLQPTMYIGDIIGITFNVYALLYEQRVKNTPQGPILEPVPSNVDITFTSIMISQTCPKEREFGCIPDKVIQDDTIGYIKTYELWRLPSRSTTSMFSFSLNHTQNAYYAIDPFGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXE8</accession>
    <name>A0A8J4PXE8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000053">
        <property type="protein sequence ID" value="KAF2076668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXE8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35885">
        <property type="entry name" value="CARBOHYDRATE BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035197520" evidence="1">
        <location>
            <begin position="24"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="316" mass="34688" checksum="5C45C0D7AA0D3593" modified="2022-05-25" version="1">MNKTLLSLIAFLCAVTFTPSAHATNYGVVISNSEDTSIQIGLVDYLNVSYAPPPSLVITNLVYNSGYYQQSTYNYNTQVLTFIATDPVTQNTFLYTVDCDEWSIINSLSFPGGAELRGLASDQTNNNNPFNIWTTAQTGNGLVIVSKIDQLSLDIVIYDILDGNYYSSTLGSNEDYFVTYGNSSGIFVNVYSTLHHKISEQKFGFSDIAETPKGPINMIYSKFTNTLLATVIVSTDNTSSFSTLAFVDRKSSTFQITTMASFANSIPEANVPDANNLNYVYSVGYVGSQYYLYSYNIYTQLLLNAAPISIPIAAAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXU1</accession>
    <name>A0A8J4PXU1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NPR2 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004579</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NPR2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000165">
        <property type="protein sequence ID" value="KAF2074094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXU1"/>
    <dbReference type="OrthoDB" id="338854at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990130">
        <property type="term" value="C:GATOR1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009348">
        <property type="entry name" value="NPR2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12991:SF10">
        <property type="entry name" value="GATOR COMPLEX PROTEIN NPRL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12991">
        <property type="entry name" value="NITROGEN PERMEASE REGULATOR 2/TUMOR SUPPRESSOR CANDIDATE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06218">
        <property type="entry name" value="NPR2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="402" mass="46119" checksum="2D51E986622A9E65" modified="2022-05-25" version="1">MDQVKCIFYSEFHNIYGTKIIQQYPEGFISNDVFEGFAEYIIPKPKLCGKVITLTAFNYKILGYPVLVQDVKYHRNALLFNLAFVFDKSIDTTPYHQIVRKLGIILRTLEKESEYLFNQSKAAVTSLSCTDLHSYGQQPPDMLKILETLYIELNSYGESTITIDDANTINVKLFPKPPDPPQVLLHQVPVLIEEIDTTNESEWDLTLLQILPFIDGINYVGRISKLTEIDINLVISCIQHLVYYNIVKMVDIFQYSNVYNPTPQIRKLASNMEMQRECQKYITIPGATPCSFEYIFTLYSSLSTDTPFRECCSENSLTSLGIDERSFIMFGVINGFLRRKQRYPLLTNLKSLESLPQSHKKHLLNGSKSFDEICCILSVGVETVEKILQEIANPDSFVTIWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PY29</accession>
    <name>A0A8J4PY29_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Molybdenum cofactor sulfurase</fullName>
            <shortName evidence="4">MCS</shortName>
            <shortName evidence="4">MOS</shortName>
            <shortName evidence="4">MoCo sulfurase</shortName>
            <ecNumber evidence="4">2.8.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Molybdenum cofactor sulfurtransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_002431</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Sulfurates the molybdenum cofactor. Sulfation of molybdenum is essential for xanthine dehydrogenase (XDH) and aldehyde oxidase (ADO) enzymes in which molybdenum cofactor is liganded by 1 oxygen and 1 sulfur atom in active form.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:42636"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:82685"/>
            <dbReference type="EC" id="2.8.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Golgi apparatus</location>
            <location evidence="5">Golgi stack membrane</location>
            <topology evidence="5">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. MOCOS subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the glycosyltransferase 10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.9" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000067">
        <property type="protein sequence ID" value="KAF2076253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10264306at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008265">
        <property type="term" value="F:molybdenum cofactor sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11660">
        <property type="entry name" value="Glycosyl transferase family 10, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03050">
        <property type="entry name" value="MOCOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055270">
        <property type="entry name" value="Glyco_tran_10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031481">
        <property type="entry name" value="Glyco_tran_10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038577">
        <property type="entry name" value="GT10-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005302">
        <property type="entry name" value="MoCF_Sase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028886">
        <property type="entry name" value="MoCo_sulfurase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005303">
        <property type="entry name" value="MOCOS_middle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045540">
        <property type="entry name" value="YegS/DAGK_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14237:SF80">
        <property type="entry name" value="MOLYBDENUM COFACTOR SULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14237">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SULFURASE MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17039">
        <property type="entry name" value="Glyco_tran_10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00852">
        <property type="entry name" value="Glyco_transf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03473">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03476">
        <property type="entry name" value="MOSC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19279">
        <property type="entry name" value="YegS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141673">
        <property type="entry name" value="MOSC N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51340">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 4" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="2 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="DAGKc" evidence="7">
        <location>
            <begin position="161"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="MOSC" evidence="8">
        <location>
            <begin position="1740"/>
            <end position="1912"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="18"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="407"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="606"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1061"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="408"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="609"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="642"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="1517"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="1357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2076253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1913" mass="216594" checksum="AA5F62B088F3B395" modified="2022-05-25" version="1">MATNYIINYDNSENNGIASNNSANTSTSGDGSGHSLNSLKPTGDKEFLHNVDNESVLYENRDAEYKKKSCSLIIKKSTLIIKYNDPKSTFTKYLVSDTIVGTSVNDRKLILSACVMKSKDNDNEVRVKKHFSFYFKSAQECLDYSNAIEQYFMDTLPRGNPKNRKIRVIINPKSGKKASESIFKDLESLFRDAKMEVKKTITKGPNHAKQIGFKFNYKKYDTIVFISGDGLFHEFINGLLARDDYEEARKVPLSLIPAGTGNGIACSIGLADPMTSALAIIRGFSKPLDVSIIQQGDQKWCSILSLTWGLVSDVDIESEKYRYLGALRLTVGAAIRILMLRIYKGKLIFLPAVDAPKDDMSKIPKCTFECDICNSDSSIDTIGNILSASSDKVKLNSSGILSQSGRLTTTTTTTTTTTTTTSLNNSMNNDNTNEAQQTSSSSSAAEGINPNSKTTEIQVPLTKIPTPNILELSEQQLTEMGYKILDGEFIGIVASTVSHLASDFIASPTAHLNDGLIDLVMIRPNAKFSKTQLLSVLTESSTGEHLKSDLIEHYKVSSLILEPGKEKEGIIAVDGEKITYGRTSMQCQRGCINLIFHQVDTQIHHTHYQPQQQQNNNNNQNENNDIEKNDNSNDKYINIEQDETKKEEKVISKEEKKQEEEEEVEEGIIPFETTTIIEQDIFFRLQTSEEEKLFNQSEPIYRDILLLDRTFYGNDKSLDSCRNLNCRIIRDRDKISTSSAVVFYVPTARRNKHPDPLVKHFNNQIYVAVSMEAPSYYPKIAELPIGFNLTFGYSYSLSQSHSNYQYLPYGPVSSTFSKWYPNNKFPSIPLSEQEFDKLTAPRPNHIMFISSNCNDKIGRIPIARKIMRVMRGRDLGINKPSLRLHSFGQCLTTRSISEKDTVKKKSKQKLFLLKNYNFVLAIENSECPDYVTEKLWQPLSTGTIPVYLGAPNIDKYLPHPKSIINIRDFESVAHLIDYLELVATNHTQRLEHLEWMRQPLSPQFQQLLNASQLNSANNLYCNICKAVQNVENGIRYSDPPTSFESLVIVILKKIIYRDNNNNNNSDNNNNEQQQQELNSSLRNSDRNNNNINNSDNSTSDIENNKEYIEFLNKYSKNNEYGYNNRINTEIRTKNEDQFPKLKDMVYLDHTASTLYSKDQVAKFSNDLLNNLYSNPHSVNSVGIKTKESVDSIRDTILSFFNAPYKGYHVIFTSGATDSLKKIGEYFPWTQNSRFYYSMESHNSVLGIREYATLKGATFFSIPSFYLKSTQEHHRIVQDLINFEQQNKVDADKEEYSLFSLPAQCNYGGTKYPLDIVNRIQKKSSNIKVLIDAASLVGTSPLDLSKYPADYIVVSFYKMFGFPTGIGALLVKNNNSSDTILHKAYFSGGTVNASLAQEQFHINRENFTQRYEDGTINYLNIIGMKYGFEIINQLGIENISTHTFALIRYLRDQLVHLVHSNGAPMVSIHADSHFADIKTQGAVLNFNVLGTNGEAIGYSEVDKLASLNNIFIRSGCFCNPGACHKYLNLSKRDIEEQIKEGHVCWDDKDIINGKVTGSLRVSLGYMSNFQDIQQFIQFIKTNFKDEHTKQSTAIDSSVNSTCLGSIEGPDNDIILSEIYVYPVKSCSGFSVEKWELVPSGLKYDREWTIIDQTGVYINQKKMPLLSQIQCSIQDNHLALQAPEMDILQIPLDYYPQSDFDQIQVCGDKVDGLLYGSKDVTLCGDVSKWLYNFTGRVCHLVRKAPSSHRYSKLLNNVQNNSSHGEKKDEISFANESPYLLINLNSVNDLKEKIYKENPNTEKSEWDWITKHSFRANFIIKGLEPYKEDEFPDFQMGNNPYICFSRMGDCNRCKMVCINQAMGIEEREPLSTLATYRRSHGKIIFGQHIQMKSMAVENNNDSPIIIEAPSSILFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYE9</accession>
    <name>A0A8J4PYE9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002429</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000067">
        <property type="protein sequence ID" value="KAF2076251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYE9"/>
    <dbReference type="OrthoDB" id="26379at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009389">
        <property type="entry name" value="DUF1045"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38081">
        <property type="entry name" value="WAP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38081:SF1">
        <property type="entry name" value="WAP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06299">
        <property type="entry name" value="DUF1045"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="LigT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035226319" evidence="1">
        <location>
            <begin position="19"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="228" mass="25638" checksum="C176EA57BB1FABE8" modified="2022-05-25" version="1">MIKTALLLLILVVASVNSISLDIHLQLDQATYDLAVYYNQQVNKLAPNNQVFLGTESLPHITLYLTDFQENQVQNIQQSIEKVFPVLQQVAQDCNVELNFINVTGQYGMWNVKLNSCLQTLSDVIVDNTYQFITPNQSIPEWVYDLPEPLRSEKIAMIQKYGSPNVFDQFQPHVTLAWDAVDNLVQVFNTINVQPASCFSPSIGIGSVGPYGTVLDNKYGLFNFTTTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYV1</accession>
    <name>A0A8J4PYV1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">18S rRNA aminocarboxypropyltransferase</fullName>
            <ecNumber evidence="6">2.5.1.157</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_006838</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Aminocarboxypropyltransferase that catalyzes the aminocarboxypropyl transfer on pseudouridine in 18S rRNA. It constitutes the last step in biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>an N(1)-methylpseudouridine in rRNA + S-adenosyl-L-methionine = N(1)-methyl-N(3)-[(3S)-3-amino-3-carboxypropyl]pseudouridine in rRNA + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63296"/>
            <dbReference type="Rhea" id="RHEA-COMP:11634"/>
            <dbReference type="Rhea" id="RHEA-COMP:16310"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
            <dbReference type="ChEBI" id="CHEBI:146234"/>
            <dbReference type="EC" id="2.5.1.157"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TDD superfamily. TSR3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.157" evidence="6"/>
    <dbReference type="EMBL" id="AJWJ01000333">
        <property type="protein sequence ID" value="KAF2071846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYV1"/>
    <dbReference type="OrthoDB" id="10262062at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106388">
        <property type="term" value="F:rRNA small subunit aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01116">
        <property type="entry name" value="TSR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022968">
        <property type="entry name" value="Tsr3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007177">
        <property type="entry name" value="Tsr3_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002621">
        <property type="entry name" value="PRK02287.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426:SF0">
        <property type="entry name" value="18S RRNA AMINOCARBOXYPROPYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN TSR3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="Fer4_RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04034">
        <property type="entry name" value="Ribo_biogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNase L inhibitor RLI-like possible metal-binding" evidence="9">
        <location>
            <begin position="62"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="16S/18S rRNA aminocarboxypropyltransferase Tsr3 C-terminal" evidence="8">
        <location>
            <begin position="99"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="241"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="246"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="271"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="345"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="367"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2071846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="376" mass="43386" checksum="F2F84F334E9E73DA" modified="2022-05-25" version="1">MSSGGKGTKNNKNKIVKHNAKHEKKSYDSDDFNARSSNVASNPDVTKGEEKHRVLALSYPIRLAMWDFGQCDSKKCTGRKLERLGYIKSINLTNKFRGIVLTPSAKQSISPADRSVVQQLGVSVVDCSWAKLDTIPFGRMKGGHDRLLPFLIAANPVNYGKPFKLSCVEAVAACLFITSFDQEGHQILSGFKWGSSFYKVNKDLFEQYKVCKDSAEVVKVQNEFIKKCEQEQKDRIENQLNHDVLEGIDDDDDEDGLANPNRYNMNYFQKDDDDDNDDYNDDDSDDDDEEEEDEDECSEDDDEEEEENGSNEVESDNEEDQDEEEEEEDDDEEEEEEEEEEDEEEIKRQYRLKKELQKKKQEERLLQKKMTKLQVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ03</accession>
    <name>A0A8J4PZ03_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CBM20 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002619</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000074">
        <property type="protein sequence ID" value="KAF2076055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ03"/>
    <dbReference type="OrthoDB" id="550577at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001070">
        <property type="term" value="F:starch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002044">
        <property type="entry name" value="CBM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32518">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32518:SF3">
        <property type="entry name" value="4-ALPHA-GLUCANOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00686">
        <property type="entry name" value="CBM_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01065">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51166">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM20" evidence="1">
        <location>
            <begin position="7"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="219" mass="24634" checksum="C167CF4162FF06BF" modified="2022-05-25" version="1">MFRCSSTSLLYGNHVTFTIVYNTQPGEILYLAGSHPQIGWDVNKAKRMTWNVGNIWDIKVGFPSVDGIYVQYKYFVYNEYSRSYRWENIENREIIINDDGMIIEDQWEVKRTLEEQVDDSTSIASSSIVNGVGNTSDLDICDDDMDVSSTANNSTHNSFTSTSILSSKVNSNTPFLYDIESPRSSLASSASSHCNNQNNAITPSSSFESRVQFPITLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ54</accession>
    <name>A0A8J4PZ54_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Autophagy-related protein 13 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003497</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000110">
        <property type="protein sequence ID" value="KAF2075192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ54"/>
    <dbReference type="OrthoDB" id="70161at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.900.10">
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    </dbReference>
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        <property type="entry name" value="Atg13_N"/>
    </dbReference>
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        <property type="entry name" value="HORMA_dom_sf"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13430:SF4">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10033">
        <property type="entry name" value="ATG13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Autophagy-related protein 13 N-terminal" evidence="3">
        <location>
            <begin position="87"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="225"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="559"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="681"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="234"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="248"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="288"/>
            <end position="309"/>
        </location>
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        <location>
            <begin position="320"/>
            <end position="332"/>
        </location>
    </feature>
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        <location>
            <begin position="333"/>
            <end position="345"/>
        </location>
    </feature>
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        <location>
            <begin position="363"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="380"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="416"/>
            <end position="431"/>
        </location>
    </feature>
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        <location>
            <begin position="432"/>
            <end position="441"/>
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            <begin position="448"/>
            <end position="463"/>
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            <begin position="484"/>
            <end position="493"/>
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        <location>
            <begin position="699"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="723" mass="79819" checksum="032A7FF374A40E2D" modified="2022-05-25" version="1">MEGLLGPKSEELTRKCISKIIQCILLSRVEVLSSETNKVTRNFHLEIKESETIQRLIETRIFSSNGIRTLFFLDIHFDENNGLGFNTLIESWKFVFEPTNQIDYSIELPKFHTNAIILVRTIYSLLRNLPCYSLFRNFSKNRSTTCSMKYQIRIADQQSINTPSFALSTPTKSFTFTSIPTPLGTIKLDVVYKENLAREISLSSQLGLESQFIIKDYNNNNFKHSNTQNIEYKSNSNNNNGYQSTSNPTRQSPPSSFNNNLYHPPSQPIPVRMHSYNDLQSLGIGTHGNNSSGSSGMSYGSSGMSRPSSYQPPNNLYDYQQQQQQQQQQQQQYNPSSFDNRNYSNPIGIPNNHMNMGGLYGQSNSPPSSHNINIHNNNNFQQPYGTSGSPPFKTGPVLPSYNRDTPPSGIKQVNFSLPSQLNPSSSSSPTSGVTVQNNIHNNPHRNRSISAPISIENNQQGINMSGGGGSSGGNAPFHHPDYPFRPSSSSQRSPPFPTSISPFKEPSTLDYYTKPTNNNNQNPLIQSSSSPSGNSFNSSIGIDRGGLIGNSGAINNNNSSSSGGGSLSNSINPKLSQLHGPVPRLDYQKSMLGGIGSNGISPTTAGALFEDDPDEPVFASTITLRNDESEIADFLKLCKIAPTLKLFEQDIVNNQQDSQSLQIQIGNDIMQLLNINFVNNNNNNNNNNNNNNNNNNNNMINKTTSPTPLSVHNHNSPTYSYPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZR6</accession>
    <name>A0A8J4PZR6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ARID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002086</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000055">
        <property type="protein sequence ID" value="KAF2076600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZR6"/>
    <dbReference type="OrthoDB" id="510958at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16100">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045147">
        <property type="entry name" value="ARI3A/B/C"/>
    </dbReference>
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        <property type="entry name" value="ARID_dom"/>
    </dbReference>
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        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038291">
        <property type="entry name" value="SAP30_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025718">
        <property type="entry name" value="SAP30_Sin3-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15348">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN ARID DOMAIN- CONTAINING PROTEIN DEAD RINGER PROTEIN B-CELL REGULATOR OF IGH TRANSCRIPTION BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15348:SF0">
        <property type="entry name" value="PROTEIN DEAD RINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13867">
        <property type="entry name" value="SAP30_Sin3_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ARID" evidence="6">
        <location>
            <begin position="15"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="149"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="149"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="201"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2076600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="415" mass="47137" checksum="79E0AF9B9D84B99B" modified="2022-05-25" version="1">MTSRENQPSNMPVDDAEEKRFIANLFSFMSNRGTPIEKIPIFDHKELNLYKLYNCVISRGGLEAVIENKLWRQITTDLAVDPERTDAGFRLRIHYLKYLYPYERKHFLKIEDDELFDYEAFERHLSKSPADKKANLSNRKKKQQLQNHFNHPNNQQHNGQQHNTNGGGQSQQQIHQQQYQQQIQQAQQQYHLQQMMNGGKSSPTPSPTSSSSSPSPISPSYSAFSPPSSSSSLYNNNNNNNNNSSSSFNNSNILNNDSNIRNNFNNNNNNISSNNAINLNNNSGSSSNNNNSNYNNNNNSNSNNSLFDNFDAADSILNIYNTSSFSTSLSNPPTSVNNLNNSNTPAVNLKLLEIKSLKKYNAYHRLKVANSTSKKELVSAVIQHFAHQNIDEDLIITSFLKRIKNEPNSRIRQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZY5</accession>
    <name>A0A8J4PZY5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_003293</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="AJWJ01000101">
        <property type="protein sequence ID" value="KAF2075414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZY5"/>
    <dbReference type="OrthoDB" id="19123at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd05122">
        <property type="entry name" value="PKc_STE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DDB_G0292350-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="927"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="103"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="350"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="143"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="1056"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="956"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="1060"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="1061"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="1074"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2075414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1217" mass="137284" checksum="27D48203971BDE09" modified="2022-05-25" version="1">MESQDIDSNTTNTMTTTTTTIIVSSDEPVVVHTSSTTTTLITDEEEDEKAAAVAYVKKERKATIRERYSKKFSISSTDSPLFTQNVNDPNFLDSSILPSFSNQPFLGTQVHQPPATPDFYSNDEYEDDDDDDDDEDDDDEDTGTTTASTATTTPTTTTTTTSTHTPQSIPQPHSPAISNKYDEEKLSNLMEAASSTVDEIEELTSSIKSDFSTGESISDNQQQRPLIDIGLEYKYRLQIEQNPKDFKTLIKWGNLIFKKVKEQLGGKDVDVCLLDWNQDLVVIPSQSTQPPPSTPFTDSLQSFLVKEPLFDACSKYQHALQISCGPNLVNIPFSTLLSFDIEKYKSELKTSSSSSTSSSTSPPLSPTSSTKSPSRPSISLGSNNQSRFLRAVSLPPAPPTPPTEDPNSPWSDPLLWMKWGDCLFLLCTFLELPMYKATCEKYFKCILLLLKKQETNEKEKEEKIELLSRAFRKWGIALSRYSRRMKCQFLMSEWTNEENIQIEELWRVLHTQSIQALNYSLEIKPSSITTFHLASAYQRHVITLNQFGGQKEAIYGLITNSCNLYYKALSMTLQLQDDSNDNGNDLNDLQKSRAIENWGQALDIQLSVKLSDEEMMEKEEEDLNSADEYLTTFGKLILRNVIPSLEGIISLCLNSTQAIQYKAINSLSVLCRSKEIQSFPIYPELKENLLKVESFVSRRDDAESLLNQQKTLKSMPPKLQAYVRMSGLGEEEIMKNFEIAWNSIYFLTKDTIPSQPIPPNYYRSNKQNKQRQKLLDSGSNIIEEKQPLIPSLHRTSSSTSFIITSPSVSIPPKFNSNGSTLSSSISSNLNNSFINNSSNSNNNNNNWNTTLPSKISTRRATVSLVSSQVEKELELEKKNQELLAKQQKQQQISIYIPKHVKRTTVNPPPLSRCDESIFSTGSPLNMFKDKIKLGTGAFGNVFYALKKSDGKPVAIKVLMERTKKGSPIIPELYIHSACNHPNIVSYMESYLVKGHLWIIMEYCDGGTVRDLLQDDWKNHQNLSNPNPLEEPLIAYIASQLLEGMVYLRSKGIIHRDIKSRNILLTRRGKVKIADFGLATTCSLGRGRTRMCGTMGRIAPEIIRREPYDTQADVYSLGCLLVEMAEGFPPYGKDSSLKALFYTAIVEYRLSNPKKFTREFVDFLQLCLLTDPFKRPTPEMLLEHTFLSGADQGRQILLDRFKGQDIRKNAILKNFVPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0Q4</accession>
    <name>A0A8J4Q0Q4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Saposin B-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_002068</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000054">
        <property type="protein sequence ID" value="KAF2076639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0Q4"/>
    <dbReference type="OrthoDB" id="19956at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.225.10">
        <property type="entry name" value="Saposin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007856">
        <property type="entry name" value="SapB_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008138">
        <property type="entry name" value="SapB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008373">
        <property type="entry name" value="Saposin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011001">
        <property type="entry name" value="Saposin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008139">
        <property type="entry name" value="SaposinB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051428">
        <property type="entry name" value="Sphingo_Act-Surfact_Prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11480:SF68">
        <property type="entry name" value="SAPOSIN B DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11480">
        <property type="entry name" value="SAPOSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05184">
        <property type="entry name" value="SapB_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03489">
        <property type="entry name" value="SapB_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01797">
        <property type="entry name" value="SAPOSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00741">
        <property type="entry name" value="SapB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47862">
        <property type="entry name" value="Saposin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50015">
        <property type="entry name" value="SAP_B"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035275736" description="Saposin B-type domain-containing protein" evidence="4">
        <location>
            <begin position="19"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="5">
        <location>
            <begin position="25"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="5">
        <location>
            <begin position="113"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="5">
        <location>
            <begin position="200"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="281"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="281"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="313"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2076639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="337" mass="35272" checksum="C5F3710A2EDA86E9" modified="2022-05-25" version="1">MMKYLVVLLLALVAVSSAYTTPKGSNAECEFCTWIVGYAEGLLANTNNTQLVVADLEKACNFVPKEYTNTCDSLVGAYAQEIIEYLLQKEKPAQICTLISFCSNSAEVEDVNGAVECSICNFFIGEVEKYIQQGATQQQVIAYLNKDCGKLGGLAGTCQALVQNYVPEIFQLLVNKENPSKVCDQVKLCDNVFVAPVEDTDIICTACEDLVNVINTYVNESEPVIINQLTQYCAKLGSGFSAICDLAVNMYGDKLINYILAGNSPSTACGAVGLCPSGSSSEPSTGSSASSSQNSNSASTGNGSSSQNSNSGSGSGSGSGSGSGSGSGSSAFHIRFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1X6</accession>
    <name>A0A8J4Q1X6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000413</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000007">
        <property type="protein sequence ID" value="KAF2078321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1X6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2078321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="561" mass="65404" checksum="6A9DA94E1C89136A" modified="2022-05-25" version="1">MFRNISRLSIYSRLLINRPILVNKNTPLVLLSTSSINNSKTCRSIFTTSCLLNNTTWTSTSSTTSNTISIDSNSNTNIDTKELDNGIKYQSYIDNAIDNFKLKQPDLTLEFLDRAVKLCPDKVDAYLLRADINDQIYFKSKHPTKQLVFDDLKKALDLSVPERSMSILISLLEWCDKNKENKLQNQYLMTAKSLDYYNIDFIKYMVENKKEKIENLLAIAKRLIDVNNNYGLYMLAKIHYFQKDYGKAEEMYHQVIQLESKRLGWSYGDQGTNMKSTNAELIHNRFLVRDIDGYRRIIEFHGDNDRDEKSTLFISFVGLAETFSKMKKPVNSYASYYQAVILKPKVPRVQFHLSELSLKLENYRQGLLHVYASLEADAVFPSTLYLDKKMLRVKLNLLGKKYVGAILDLEFLAIQYTQEHDFHIGLFIQNINLMNRIFPRIIKDSNEEKDTHFTTKIKQPLMLKHDPKGIMAVKPTDLIQLQLHALLLKGVYMDLKKEQMDPQSLNAVYSKLHFVTYVTFSLSKAFYYFGIAQNNKYYAKLTLSLKKQLQLYNKFFFDFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1Y7</accession>
    <name>A0A8J4Q1Y7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_001380</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000032">
        <property type="protein sequence ID" value="KAF2077314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="19795at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034517">
        <property type="term" value="P:ribophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040040">
        <property type="entry name" value="ATG11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045326">
        <property type="entry name" value="ATG17-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13222">
        <property type="entry name" value="RB1-INDUCIBLE COILED-COIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13222:SF1">
        <property type="entry name" value="RB1-INDUCIBLE COILED-COIL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04108">
        <property type="entry name" value="ATG17_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0072">Autophagy</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="4">
        <location>
            <begin position="37"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="523"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="691"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="909"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="551"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2077314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1393" mass="160295" checksum="02A2B3578945F2F6" modified="2022-05-25" version="1">MTSPFIVDSSGGGVGGGSNSFINSNNTNNNNQMQASSTFVLRIINSQNGHVYKIPANPAIVVDDIKNTLSQMTNIPKSDQRLLTNDANLNSVRNLASYNIYQDREFFLFDRPNSKKPDEEPVKTSEIIASQIPQKPQPKGFKDYETTANPINRLFGIEFLLENQLSAITYIKDAYEKNHGYCYNIQKEIENQLRAIDFLTSFSNDYKKKVTHYTKNIIGEFQNTLPNYESLLNSFDLDMNRLRQIRLHDCFKRPNQSLADCVDEKSHRDWNNDFQSSLNHLKGKALEVENNLQKTTEVDVNRVPESIQQIKNNLGQIQTVKEHYIAVHTQYQYFFKNHQDSKRALEQIRSNNDGSNVNQISTILLKFLEIKNAQANTLKEIMANQDVARITNSFFKLKNFVNHQVWEQVQIVAMLEDRIFSVNTKVLLFKEAFYSQIEKSAQLKYIRNLPTAYESSLTEIASRKEFGSTINNTLGKFSDTLKNIRDKEAQRRKKFSEQTYPSELFYFLKDQLPNLTLTLHPPKFDERLPNINNIDNSDHKRISSGSGGIGSSLSNYNNQNQINNSNYNNNYQNNNYNFNSSNSNHSNNSNNSNNSNSNNSNNDIEEEFTVVDDNKLNVQVQEDYESKKKSTQIQQQQQQQQFEKSVSELNLNESLQIKTLESKNKTAFNLVSKLSVYKDLCESQQQQLTQLGVVSKELEEKKQEIVNLTEKIANLILENKETEKLNTTLESAQLELESKIASYLAKLEESDKVIDQLNQSLQQLKDQQTLELAKKDEIITQLQADIVAKEQEINEAQDMINRHEDFIKISTNDTNDSKIQLESVSLEKEKLALEKEKLATKVKEQEELHKKAYALKEEEVKNLAIAMSQLQDELCELRASLQESFEKHEQLAKEREIESEQVVTLRSELDSHVKSISEKEQEIKLYKDQIDQSFKEKESNQEFNKEQLAKIQVYQSKIETLELDHKTSIDEINQLKEKQANLLDKISQLEIEVDASRSTMNTKQSESLESQKQLGLLEHELAKFKELFENLKQEKQKTVDQLESSQLDNKSHRESFENEIKEKNNEIKLLKNALNDSSIDSNRQREKISQLEEEIQSLRSTLQTLYNQLETEQNEYQANFKSKDTIISMLNAQIEQMNTEASNTKEDSAVTDRLNAEIKDLQSHLSHLQTLLTKSENLSNQNQKESEQNKQKCDKLARDKIELLNEVESKSSEISHLFDEIKSLKHTLLQISKNVGLSNTSNEMNDYSMLIQEVAKYPIVQDNCSMLEHALEQNTSYVQELKKKISQLESTQVEMFLSKEKEVSASLSNFTSGSTALFCKNHNDVYEAVNIGCPNYFLSRDSFEIFKNEMTKGIPILGIIFEINSEKISSPSDSYGVPPGTRIHEVTLGKHFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q278</accession>
    <name>A0A8J4Q278_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Extracellular matrix protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000731</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000015">
        <property type="protein sequence ID" value="KAF2077930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6406428at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052846">
        <property type="entry name" value="ECM-enzyme_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001673">
        <property type="entry name" value="S_mold_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31797">
        <property type="entry name" value="EXTRACELLULAR MATRIX PROTEIN A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31797:SF3">
        <property type="entry name" value="EXTRACELLULAR MATRIX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00526">
        <property type="entry name" value="Dicty_CTDC"/>
        <property type="match status" value="47"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035316317" description="Extracellular matrix protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="1282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1282" mass="135629" checksum="C42414869CF1E14D" modified="2022-05-25" version="1">MIKYIFILLIVLSVFGLAFANVENAEEVTVCGTGTCGSCVGGCDDKNLCTTDTCIAGKCFNTPKLCMTILRCGVATCDPTCGECVVTGPKDSSCCDDGSACTIDTWSNKTGCINTPRSCDDGNPCTADSCDCDKGCQNVPINVDDNDACTRDSCSNGKVIHEKINCDDGNSCTIDSCDCVKGCQNVPKNCNDNNPCTIDSCSNSTGCVNTAIDVNDNDACTRDSCSNGKAVHEQISCDDGNACTVDSCDCVKGCQHTPKSCDDSNPCTIDSCSNSTGCVNIPMDVNDNDACTRDSCSNGKVIHEKISCDDGNSCTIDSCDCVKGCMNINKDCNDNNPCTIDSCSNSTGCVNIPMDVNDNDVCTKDSCSNGKVIHEPISCDDGNSCTIDSCDCVKGCQHTPKSCDDSNPCTIDSCSNSTGCVNIPMDVNDNDVCTNDKCVNGKVVHEQISCDDGNSCTIDSCDCVKGCQHTPKNCNDNNPCTIDSCSNSTGCVNIPMDVNDNDVCTRDSCVNGKVVHEPISCDDKNSCTVDSCDCVKGCMNINKDCNDNNPCTIDSCSNSTGCVNIPMDVNDNDVCTKDSCSNGKVIHEPISCDDGNSCTIDSCDCVKGCQHTPKSCDDSNPCTIDSCSNSTGCVNTPMDVNDNDVCTRDSCSNGKVIHEKINCDDGNLCTIDSCDCVKGCQHTPKSCDDSNPCTIDSCSNSTGCVNIPMDVNDNDVCTKDSCVNGKVVHEKISCDDGNSCTIDSCDCVKGCLHINKDCNDFNPCTIDSCSNSTGCVNINMDVNDNDVCTKDSCVNGKVIHEQISCDDGNPCTLDTCDCVKGCQHTPINVDDNDACTRDSCVNGKPVHEKISCDDKNSCTVDSCDCVKGCMNINKNCDDNNPCTIDSCSNSTGCVNIPMDVNDNDVCTKDSCVNGKVVHEKITCDDGNACTMDSCDCVKGCQYVPKSCNDNNGCTIDSCSNSTGCINTPINVNDNDACTKDSCSNGLVIHEKITCNDNNACTDDSCDCVKGCVFTPKNCGKSDRCNIVSCSNSTGCVRVPVSCDDFNPCTVDSCDAIRGCINTPINVDDNNLCTRDSCVNGKVVNQPIVCDDKKACTNDYCDPKRGCVFAPVVCNSTSCSKGSCCEDTGKCVYENTRNCDDNNKCTIDKCDPVKGCTNTNLVCNDNDPCTDDSCDCGRGCVFTPKKCEGKDKCSKYTCDKASGQCKAEKIVCKPSSNPCLVSFCNPECGTCDTKPLVCDDGNPKTKDSCSIAKGGCVYEDCKDDDDKKPTTGAKTIGGFKWPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2B0</accession>
    <name>A0A8J4Q2B0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001274</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000029">
        <property type="protein sequence ID" value="KAF2077425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2B0"/>
    <dbReference type="OrthoDB" id="21473at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="173"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="277"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="390"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="422"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="60"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="97"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="121"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="173"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="189"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="204"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="284"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="510" mass="57570" checksum="336478FE134BAD56" modified="2022-05-25" version="1">MMMDVDNAKKNSPENEEKKMVVDQIVDEDDKKDDQQQKETSGADKPSNQEEVQQQQQNKEIVKENKEENEKENQGNNNNDKKANEESLDVDVCTIDSKNDQKMEEKDRDETIDLVKSVIINLSNSNSSENTSSSSSSKPPIKFVNSSAKPPMEQPRSESPIENFVVIKNSLKSSSSSLNNSNTNNIVNDSQPPFSQQQNRNDEDALEIDHEDSDPMITDKSSSSNSMVFSKLSSSSGSSNFTNIESEQLYLASKLQNIQQNNQQQQQLLSKSQPQLQTLNNKNSSPSQQAPQQQPQTPLQQQTSTTTTTTTTSNRGRKRNPTVEEKRIQQQQLQQQQMFLGNKGAPGSSNLQYNINSSLFPNNLNQSNSNHMFPNNITSSSNFNMINDTNDIDNKRKRSPVDFQNDENLSNHTSNQSISFGIRSLVNRIERMEREREILINENKKLRKEVSELETLGAKATSTLTQLMEDTSRLKNSKELLTNRNALLQQTNDIIQTQLKTLLSCCSPTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3A9</accession>
    <name>A0A8J4Q3A9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aquaporin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_001295</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000030">
        <property type="protein sequence ID" value="KAF2077367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3A9"/>
    <dbReference type="OrthoDB" id="18905at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139:SF290">
        <property type="entry name" value="AQUAPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139">
        <property type="entry name" value="AQUAPORIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="89"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="261"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="303"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="14"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="31"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2077367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="36385" checksum="9A6BF0B97AF80540" modified="2022-05-25" version="1">MESNSNSNNTDIELNNINDNSNLSINNNNNGTRVISRNNSTTPKITENYHTTVGENVEPEKEKQLTWNEKFKKILGLDELRSKKMYQGVIIEFIGTIIYVFISVSIVVSCVNYYVYSADYNANSPLQVILNTPDSVISPKLTITVALAHIIVLGLMILTTTPHSGGHLNPTFTIASCLTGFTPVFRTIFYVFAQLTGSIIGSALVKSMLPDFISERSVLGACLYGNSMTTSGVIVGEFLFTSFNLFVAFNMGSGYSKLVPPFIISSTLAVSFVGAQGLINNYFGPGVNIARCFGPAVVMHDLSSFWVFVVGPLMACVFISLVNHIVPPHSDYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3T4</accession>
    <name>A0A8J4Q3T4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000425</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000008">
        <property type="protein sequence ID" value="KAF2078235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3T4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32457">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32457:SF62">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="883"/>
            <end position="901"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="902" mass="106007" checksum="09EAFE879F6F8564" modified="2022-05-25" version="1">MINFNNQLFFYIWKNKYLNHTIWYQLKQQSNQHIYNIKNFEECHSLNWMLQNGYLELLEYRLKRNDYISVDSNNVEDLIKTIFTDININNQLEDSSNNNSNSNISKEKIFNFLKLVINRLSFIFSTTKASLDIAISCKNYLAIVILCQDLKVLVEKSSLDLAISLGDKKTLEYLLLFQNGTFNISCALKKMIQISNNNEKLRDNMIEYLLECPFFDIDQLTVDRLCPRDTIFIEDLLYSENLNLIKSLLKKNLIKYSSNSFIKGVSNISINYNQKLLLDVNNQNNNKYQYSQIVYEKYENNGNINSNINIQCNNNNSSQNNNNSNQNNNNNINPIYPTLEGKKDLNIIKTIVYIYNKCYCKEKKDLKPISLDQDLERLEEEDNPNIAFLKYIVFPLESILGKKYTVKNLIHYSMLSGSINLIKSLFKKENPSLYTKSMIESPDWVLLLEYSITHINIDFLKFALTNSISEGFTFWSSLDLIDQLNIHFSVEKYQFHSIQMVSNLTHFLTTLVKRCIKTSNKQLINLFIQFLVGVGNLKVLEIFYQTILNLSPIAFLPSILYINTQILERIPIESREQIYHFLFTPEYEIQFFQTRLLMGIKENVNINVHQILKNAFIRGDIQLVRVLVQKGNAIRDEYLINAIQRNQYQVVEYVIKTMRYNPTSLSDLINCAIVYDISILNLFESLLAPNTPKKEINEEYNIGYYLYNLLKAGKLNCFVHHIQTLISNNRIKLITFHLSDFSIFRIIGMIGNTNLVKFIDNVIKQISLSTYMYKLILYGAFSKGHLHIVRYLFENELVVASLKNYDNHISISQIFYHYLEYNNSYDNEMLEYILQLYKPISKPIDFDEQINLLYNQFKKKKRLHTCQYLRDRYPTSTKTISKYPFLIGIYIIILFLILSSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4G1</accession>
    <name>A0A8J4Q4G1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_000035</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000001">
        <property type="protein sequence ID" value="KAF2078664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4G1"/>
    <dbReference type="OrthoDB" id="10251508at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045016">
        <property type="term" value="P:mitochondrial magnesium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12823">
        <property type="entry name" value="Mrs2_Mfm1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039204">
        <property type="entry name" value="MRS2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890:SF0">
        <property type="entry name" value="MAGNESIUM TRANSPORTER MRS2 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890">
        <property type="entry name" value="RNA SPLICING PROTEIN MRS2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22099">
        <property type="entry name" value="MRS2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="430"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="90"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="347"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="90"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2078664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="692" mass="79395" checksum="45B72317D7A80BA8" modified="2022-05-25" version="1">MNSNSILLSYNYGIKSSIRSSKTALKHLNTSKLFIKSNYQQQKRSYVETNLRPPFLFKPPIELNGSHSNNSNINFQDFSNSFLFTKNSSSNSLNHNNNNNNNFTSTNSNNNSNTNLASPRIFDQEYFQHGNVNGGNINQANETLKSFKVTCIDINGNPTETKMYKSDLANELKLQARDLRTVDPSFPPQMPTILVRDKVILISIGCVRAIIQYNRVIIFETQNQTVKEEIMSLIRDSLNSTTYEVLPLPFEFKVFESMLDISCKKLEAEYRRMQSLVERELQLLNNNPEHNLEDLFLYHKKGLNQFEVTIKEIIDAITDLLQSDEDMALMYLSFRNATGGARRKHQHDEIEILLETYTRQLEQMSSNIAQLKETLNSTEEFVNFQLDTVRNKMMRMNLMLSLVTISTGFGGIITGTFGMNLSTGLESHPFAFWIACGAISVIGSLTFMGLRYYCQVKNILPYTKKTPARLKIGSGFRNDSFVNPLSEPSSQDLLFVDPFGIASNSSTINNRSLIGNNNNINNNNINNNNNSNNNITTSQNIQNNQQRQTLLNQQYRQQAQNDFNRIKTENMKSLNSFSQREILDQIHHIQKQIQQQQIQLSHLQHLSQSDYPLNLNYINNNSTLEYKDHIPSPKDDVNQSEYEYHKKLSHSFLKSKPVCEQPPQVKSLSKLWKHINDDTTTNQKNNSTPIQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4H5</accession>
    <name>A0A8J4Q4H5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">2-(3-amino-3-carboxypropyl)histidine synthase subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_000620</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2. DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase. Facilitates the reduction of the catalytic iron-sulfur cluster found in the DPH1 subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the DPH1/DPH2 family. DPH2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000012">
        <property type="protein sequence ID" value="KAF2078069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4H5"/>
    <dbReference type="OrthoDB" id="449241at2759"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11840:FF:000002">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11860:FF:000001">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11840">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010014">
        <property type="entry name" value="DHP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042263">
        <property type="entry name" value="DPH1/DPH2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00272">
        <property type="entry name" value="DPH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762:SF2">
        <property type="entry name" value="2-(3-AMINO-3-CARBOXYPROPYL)HISTIDINE SYNTHASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01121">
        <property type="entry name" value="Diphthamide_biosynthesis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00408">
        <property type="entry name" value="Diphthamide_biosynthesis_famil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2078069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="596" mass="66913" checksum="1A6CC8ADD375907E" modified="2022-05-25" version="1">MSDESTSHVIPTFSTESFNVSSNRDVMEDTGMGDAEHIPTESQFDAGEYDFDTYFQIDESIRVIKQYSFNKVALQIPDYLLWASSRISNSIQQQVPTTKAFVLGDTSYGSCCVDEVTSSHLRSDFIIHYGHSCLSPSSKIPVQYVFGMKKDLDIQQLKDTIAKDSSQKYIVLYELFYHHQRDQLVNEFKDNQNIVIGDIALYNNIFNNLKNNNNSNNNNNKVEEEENKDCCSKSNSCCSTTTTTTSNCNNNNRDSCCSTTTNNDSCCKSTISTSTSTTTKDITNSNSVMIQDNEFIVKILGRSVTIPKDNDNSNSLDVNSIIKGYNFLWIGQECITLTNLLMNFNQNNVYRYNPKQNKLDRETLPRNKSLMRRYHISEKCKDANIIGIVVATLAVEKYAETIDGLKKLILKSGKKPYVFVVGRLNVPKLANFSEIDIYVVVACPENTVIDSKDYYKPIATPFELNLALRGKQWTGEYETDFGRVFPNLIEDIDADERYRAEDEASDDEGNKIHFSLVSGKIVHSTNTAASSVAAPSETSLVAADDAFKQLSVAQDHHTQRTYKGLDMRIGETPITAAVTGLSGVPRNYDTDKQNLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q5C1</accession>
    <name>A0A8J4Q5C1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000094</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000002">
        <property type="protein sequence ID" value="KAF2078594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q5C1"/>
    <dbReference type="OrthoDB" id="24472at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2078594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="749" mass="88111" checksum="290AD24250EF6E4C" modified="2022-05-25" version="1">MHTTTVTTTPTLLFFSIFKNKFLAKKILDLCKCQDRVAYAYYDIPFALLFKTKRFAIFDHRWSLGAKIIHKSDTSGSNYDDEHLKYIFDVRYQDVIAFFSNNDCIDRLKRVWKLFFQIVKANVDIMRHDDMDTILAHLLLLDANRELELYWKFYKKNISSSYMFGAQQTPDLLTKIIGQHGSSRVYDQAFRLLGAEITYKCMKYASQFGNIDLFHHIFQQAATKQNRIIYYLADFKELSGFLLANVDSSLLRVSYLPWDGMCTTDYDFIEKIIQKFKPTINFPKAYEIGDLKLLDYLCNRYSHLPNRIVVGSHLANFETFQWLLEKSPTLNPISDIEYACQHSVMFYKHLAAHGFNFTAKTFDAPYPILEYIDQTNAWPAQFSSAFIKKNYHQDIRIIKLLHRVYKDDFQVSVADFKTFVSRGCVDIVKYLIENESSKLLFIELYQLETLIFSINNNELVHLFYQVISFHKPHFAEQFVTQVKNGFICLASMEIIKKVTKCKCLLVELCLLYDRMAQIYNLDIPVVYEYLLDGFDQEQWNGNYLTVALLHAIRIGNVKFIRYITNLISRLSAQISIKTENLFINFGQGFFGKTLLETYVHWNQETLVQILCSKYMYTPLLWFVLSHVNDQDQYTHLLDVTINTLKAEKKYDALQQLVRCLKTPLPWRSEPQVNNRVKVHPPFKSKTNFHLKTSNYLVSNQIFSESEIFGSFEIDPKKYTFDETNTYDHSQETEIFGFNRDTFKFDFDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UP87</accession>
    <name>A0A8J4UP87_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009809</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000815">
        <property type="protein sequence ID" value="KAF2068866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UP87"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="54" mass="5883" checksum="B8B36392D8A0576D" modified="2022-05-25" version="1" fragment="single">EKCSSSGMPTWKLAVIIVCSVIGASIIAAVIVMLLKKNNKVRAVAFKLKLRNRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQM1</accession>
    <name>A0A8J4UQM1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carbohydrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007817</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000438">
        <property type="protein sequence ID" value="KAF2070861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQM1"/>
    <dbReference type="OrthoDB" id="15614at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019028">
        <property type="entry name" value="CBM_49"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052879">
        <property type="entry name" value="Dd_Spore_Germination_Stalk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33239:SF6">
        <property type="entry name" value="CARBOHYDRATE BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33239">
        <property type="entry name" value="CELLULOSE-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09478">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01063">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035272870" description="Carbohydrate binding domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate binding" evidence="2">
        <location>
            <begin position="56"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="150" mass="16836" checksum="E5D29E6528C123B2" modified="2022-05-25" version="1">MFNRSISFIFFGTLLFLTIINSYEICGPRSCKANSFCISDKTAALCRPIDQAATLIEYNITADGVWTTSSQYSGSVKNLCNVKVRKVVFHADLKLIKPNSIWNAKYNPERNEIEVPDFADGLKVDAESYTFGFVLASTDTPEIKIKSIRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQV3</accession>
    <name>A0A8J4UQV3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pmp22 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_007474</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000401">
        <property type="protein sequence ID" value="KAF2071206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQV3"/>
    <dbReference type="OrthoDB" id="430207at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF17">
        <property type="entry name" value="PROTEIN MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2071206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="22046" checksum="519A9AEDE71A15B8" modified="2022-05-25" version="1">MSRYWRGYLHLLDKYPLTTKSLSTGVLMGTGDILAQRIDFHFKQQSLNKDNNNTNDDNRFKIDYQRVLTMTSVGVLFSGPVLHYWYTSLDKIVVGSGKTVYIKKMLLDQGVFAPIVISCFMGIMNTIHGKSLADTEQKIKNDLFYAMKANWMLWPAAQLINFSLVPPNLRVLYTSVVSIFWNIFLSHLGHKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UR45</accession>
    <name>A0A8J4UR45_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_007086</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000359">
        <property type="protein sequence ID" value="KAF2071596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UR45"/>
    <dbReference type="OrthoDB" id="674948at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14704">
        <property type="entry name" value="bZIP_HY5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47416:SF8">
        <property type="entry name" value="BASIC-LEUCINE ZIPPER TRANSCRIPTION FACTOR E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47416">
        <property type="entry name" value="BASIC-LEUCINE ZIPPER TRANSCRIPTION FACTOR F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="9">
        <location>
            <begin position="159"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="32"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="261"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="184"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="49"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="123"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="133"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="261"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2071596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="293" mass="34047" checksum="1CF8352CBADBF1CA" modified="2022-05-25" version="1">MEGNIQFTYQQQQQLIEQQLILDQIYAQQQELLQQQQQQQLDNSNIMTQQQQQQQQQSSSSGIQQKYPTRKYKKQKDKLAELGIHVELSKKPEPSTRKRQPKSKPAQPPAKESKAAAKKKKEKEKEKEKEKEQEDDYTDSSDDDNHDEDDSSDENEDEESKKTRRLLKNREAAQLFRQRQKEYITNLETKAASLESSNNNAMSKVNVLSEENQIMKDKVKYLKTFVKQAVSMSLPVNHILNPNQISIPNNSNNINPFSNINNNNNNNNTNHPNNNTNYFNNNNNNNTRGLEPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4URY7</accession>
    <name>A0A8J4URY7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005773</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000239">
        <property type="protein sequence ID" value="KAF2072916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4URY7"/>
    <dbReference type="OrthoDB" id="6507044at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="337" mass="38204" checksum="02E32CA153E0FC9C" modified="2022-05-25" version="1">MSENNTNNSGNGEINNSNNNSRFSIDRNKDIITVDSTHTKRWVEYGAYLNARSKVVLEGGGCNPHPYERQVSRDVFCMDAVEWVKQNEIGQDASIITSLPDITEISNFTLEEYKLWFRETVQLICSKLTDTNVAIFYQTDIKKRCKKSNGRFDQYVDKGYLCQRGAEEAGCKVVWHKIMTASDKPFVSRNKACFSHMICVAKSPRLLDFEDSTPDINRRGDMIWSRAMGLNSCKVAIMYLKSLNMKCVVDPFCGKGSILAVANLYGMNSIGVDLSVAKSRESIALTLDPILIETFSNRYCKVLEKKQSGESVVIDDTEEPEDQQDQQDQDQDKEEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UT57</accession>
    <name>A0A8J4UT57_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_004243</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000146">
        <property type="protein sequence ID" value="KAF2074461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UT57"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053331">
        <property type="entry name" value="EGF-like_comC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24032">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035298956" evidence="3">
        <location>
            <begin position="25"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="608"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="560"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2074461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="627" mass="71541" checksum="B93175E5B54E2C24" modified="2022-05-25" version="1">MFKSKLLLLCIVVILVISIKSTRALVQSEYESLISLLNKIEQNTTTYNELNICDFQACQKEEPDCINYFLCDDDNDRITDLNIKGEGNISLSANDFNFPLLKFLSIDSFNVETDFLYLLNTKAPLINTLQCSYCNISSIRIGTSYNTLYLNHSNFQGSLNLSSIINITNLYLYDDVVWVNDIVPSDIINTYYMKFKTSIFPDLTYFNSLESISIYPTSNEIDFSNLNKAPHIMDFEFKRDKKESLSIRLPQSLVNYKPSMELKSSFWKFHSENINWILDEPIDFSKTYIKLFESRDGTIKLNNQESKFPFSKGTLISTLKINNANFNEIDFNVLVNTQSSNFQNNNISMQVGNITLTDYQIDINIDQNKIYGPVPNWFCNLDISFANNQFQGDLPSCYTCFLNNALIRDKVKGNSFSNYNDDMTPSQYPPCKTLVIHYYLLAHVNPNSTLYSLDTLYVFYVFGHDLGINSNIKIAQDDGFGLFSIKMLKPNEIYFVELNEYNYKKLKNISNTFHDKGPLTITFVDPGITIMMNVSNVLDGEILEWNSTLLDPFMYDHENPSTKMPIETSTPSPTNTPPPQQGTSTSPASYEYSNETIDLGPKLSLAPLFSPSLISIISLSLVILILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UTY5</accession>
    <name>A0A8J4UTY5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010305</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001029">
        <property type="protein sequence ID" value="KAF2068368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UTY5"/>
    <dbReference type="OrthoDB" id="1415503at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="514" mass="58711" checksum="D9936C97FD94A431" modified="2022-05-25" version="1">MNNNQLFFSIFRNISLKRIICSLIVKDITIDAHPDYLDDNHQYLQNLICGQEYKLQINASITNESIQQFKLCKHRDLYTSINFSSSLPKEIHFDIVANYLPKYIQIISNINEHVLHQKQLRDIPTLTNLSCHPLVDKLKPNTLPINLEFFTVSLNNGSIEKDVLPQNMKSISIRDQCHKNKDTYLNSVFPHKMDAHLGVIVDYDNPLEYHRTNVNITSVYIEYLRYEPLSANLFPPTVKKICLMNFSSSELQIGCIPQCATNLLIGTLGGELRIGVLSTHKFLTSLDIMTYNGPLLPGVFPKSLTTLCLSSYDIQLQPKVIPQSIKELYLTSYSHNFDNNVLPMHSTVFDELCLYGSPILKKGSLPNCIKTLRIRADNVQSSDIYPASLNKLCLVDCNKENLKIVLKSLPHSIVCLEINFDDTAINHLPPLPLSITNLKLNCFNEFKIDSLEFLPPKLQKLTLNLNFNSSIFIPPKTLPLSLEVIMITSQKNIQFKPPPTVRTYINNRYYQKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWG0</accession>
    <name>A0A8J4UWG0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_009160</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000646">
        <property type="protein sequence ID" value="KAF2069523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10255969at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031288">
        <property type="term" value="P:sorocarp morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002530">
        <property type="entry name" value="ABC transporter A family member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000298">
        <property type="entry name" value="ATP-binding cassette sub-family A member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056264">
        <property type="entry name" value="R2_ABCA1-4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF273">
        <property type="entry name" value="ABC TRANSPORTER A FAMILY MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23321">
        <property type="entry name" value="R1_ABCA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="24"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="231"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="307"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="368"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="406"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="850"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1014"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1056"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1094"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1126"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1159"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1204"/>
            <end position="1227"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="481"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="1273"/>
            <end position="1508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2069523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1608" mass="179782" checksum="44D9A501EEE5B791" modified="2022-05-25" version="1">MGEFKNQLKVLIQKNFMLKKKSKCGMICEVVFPIVIIVILFLMLSLILAFKDNYDINNLSTFGYRVGQNKVLLYGSNSTFSIEQQGVINVLRGLVAKQQNITFEATEAMFIEMNDHLAMENYFANNSKSVLGAVWFDSNVSPSANNVPFKYNIRVDSDKVRNNLKQFEEDMDSLVYAQDGFGFIQVAVDQAILQFYNSSQLLNVESQLFPDPFTEGWQRWHTGRDAIMKDAGGVFITAALFMFSFRLITDLVIEKETKIREGMKMMGLKETAYFLSWSVTHLTFSLPVTVLIVLIVKGSQLYHVTNWGIVFVLFILYLLTLLLLSFILSIFFDKSKFAGIISMVIIILIAVAGIFIAKEKFTVDAKLFLSLFSPIAFACATYTMTMKDLNGIVSYDPKYLVEESHIIGMLVLDIFFYAFLVWYLDNVIPGEYGTKRPWYFIFTKSFWMNISARNTSSDIESTYDNQDVELIPVDIRNKTTVSIRNLRKEFQTGDGLRVAVNELYLDMYQDQIHAFLGPNGSGKSTTIGMLTGLFPPTSGNAFIQDNSILTDMSKVRRTLGVCQQTDTVFEKLSVYEHLVIYAALKGVDQKAIKSEAEKMAIEVGLGEKMHAAAGTLSGGQKRKLCLGIAFIGRSEVIFLDEPTSGMDPLSRRGVWDFLLKYKKGRTIILTTHFMDEADFLGDRIAIISRGKLRCDGSSLYLKNKFGVGYLLTMSKETGRCDTNNVSSFVHRYIPEALILSDAGTELSYRLPTSSMPSFVNFFRDLDSNKHNLGISTFGISVTTMEEVFLRIGQESDNANNKQFNLNSNENFNDEKAKKAISTPSAGVSATQQLKGLLIKRLRTSYKDLKSFILAILIPAVVIIGSIIVYKKVDSGREYYNSVTTPLTFSLGLYQSDNRVPISTLDNNVNALNASAYKDLLVPVPGAQLEQYLIDNYKTDVGALNVSSAWDDSMLSYKALYNTEYLHSLPIHINFVHDALLRKHNGIGIETTSWPFAHVLTAIEQALADIKVDAILYFIILFTAGWALMAASFAGNICYERVYRVKRLLYISGCKKYVYWLSNLIWDYFFALILLFISVGVLAGVEPKFKEHFGLIFLATLLYCICIIPLAYLLSYRFDSNGKATGAIFGVLFGMGLVFIIVTINLRIQVVSEQSETIQLAADILDFIFFITSPLYCLAKVAFIITDFPGAKRLGQWEIDNYWGMLFGGGPILLLAIHCVIWLAWIFILDYMGEIKGYIKNPKTVQPPAPESNEDSDVALERQRLLSPVIDDVVYMKNLHKSFPPRGKAPTKVAVYNTTLGIPKGQTFGLLGLNGAGKSSSISLLSGDIVPTAGEMFINGHDVVGEKSKALSSIGSCPQFDALSPLLSGREQLWLYCRIKGIPESQIHDVVESFISMMDLERIGHSNVSGYSGGNKRKVSLSIAMLGNPSVVFLDEASTGCDPQVRRFMWNVISELGKDKVIVLTTHSMEECEALCQRVTIMKDGKLTCLGSIQHVKDKFGSGYCVDVKFKKEYLENGVDIVRKSFPSATLLDRHDLVANFELANPPTNPVNVSEIFATLQNQLANILDDYSVSQTSLEQVFLKLTGANYEARLNQMSEIPVDPLSLPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWR1</accession>
    <name>A0A8J4UWR1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000912</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000019">
        <property type="protein sequence ID" value="KAF2077791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWR1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="105" mass="12157" checksum="B3F028243EF43CA4" modified="2022-05-25" version="1">MTSASDYWEDHLIPSVLFPTMDYVYNKYFKSNKNQIETGNLKSSTSTPKTKQQECSNDYYPLSRNIKLNNSCKLDLPIEENSKNSHNTIPQSTCPLVKVFINMHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXX5</accession>
    <name>A0A8J4UXX5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phenylalanine--tRNA ligase</fullName>
            <ecNumber evidence="5">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Phenylalanyl-tRNA synthetase beta subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_007604</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="5"/>
    <dbReference type="EMBL" id="AJWJ01000415">
        <property type="protein sequence ID" value="KAF2071083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXX5"/>
    <dbReference type="OrthoDB" id="1698572at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.10:FF:000005">
        <property type="entry name" value="Phenylalanine--tRNA ligase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.40.10:FF:000002">
        <property type="entry name" value="phenylalanine--tRNA ligase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.10:FF:000004">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="entry name" value="Phenylalanyl-trna Synthetase, Chain B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004531">
        <property type="entry name" value="Phe-tRNA-synth_IIc_bsu_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040659">
        <property type="entry name" value="PhetRS_B1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00471">
        <property type="entry name" value="pheT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947:SF0">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE BETA CHAIN AND LEUCINE-RICH REPEAT-CONTAINING PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18262">
        <property type="entry name" value="PhetRS_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56037">
        <property type="entry name" value="PheT/TilS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B5" evidence="16">
        <location>
            <begin position="303"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2071083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="611" mass="68164" checksum="3F714BF8CEF90E26" modified="2022-05-25" version="1">MPKVNINRDLLFKALGQTYTEAEFEDLCFSYGVELDEVTSEREMILKETGVDNANVSDEVIYKIDVPANRYDLLCLEGIARALNVYNGKQKIPKYIITPPKNGVYEKFVVEKSTSTVRPIAVCGILRDITFTQEVYDSFIDLQEKLHQNICRKRTLVSIGTHDLDQIQGPFAYKALTPKDIKFVPLSQTKEYNAEELFDFYDKSNSHLKKFLPIIKDSPTYPVIYDSKNTVLSLPPIINGEKSKITLNTKNVFIEVTATDTTKANIVLNIMLTMFSQYCKVPFSMEQVEVIDAQGKSQLYPQIQDRLIDAKADYINKCVGIDITSEAMVKMLERMSLESKLSNDKQTITVAVPPTRSDIIHAADVVEDVAIGYGYNNLKKVIPATMTTGRIQPINKLSELLSNEVALAGFTEIMTFVLCQNADNFGFVNKKDDGSSVKISNYLSEEFSEIRTSLVSTLLKSVVANKAAPLPLKLFEVSDVSVLGSTGNTDLSDPNSNNSDVGAYNKRMLGAIFCNNSARIEVVHGLLDRVMMALNIKQDITRSGNGYYLEISDDKLFVPGMGTNVIYNGKKIGVMGVVHPTVLKNYSCPFPCSLLEIEVTMDLAKNILFRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYH4</accession>
    <name>A0A8J4UYH4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Gamma-secretase-activating protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005911</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000249">
        <property type="protein sequence ID" value="KAF2072765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYH4"/>
    <dbReference type="OrthoDB" id="19866at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902004">
        <property type="term" value="P:positive regulation of amyloid-beta formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028010">
        <property type="entry name" value="GSAP_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026172">
        <property type="entry name" value="GSAP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13630">
        <property type="entry name" value="GAMMA-SECRETASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13630:SF1">
        <property type="entry name" value="GAMMA-SECRETASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14959">
        <property type="entry name" value="GSAP-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma-secretase-activating protein C-terminal" evidence="2">
        <location>
            <begin position="817"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1009"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1092"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1009"/>
            <end position="1021"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1157" mass="130337" checksum="C7A67471BB3A9BDF" modified="2022-05-25" version="1">MFKFRSIFSLEHDVIPRIPSDQFPPPESLIKKRCSLDNQQQQQPQQTYQIPIQQQQQQQQQQFSLPIYSHLNNNNNNNNGSPSKMVVLQQQIKLGSSLSSAPIHFSNDGDQDNNNSNNNNNSSISSNSNNNTEQQQQIDYIQSINLRIIGRERNGILLLGRTSKVDGVLKTYIDTFDISKKDFNNMCVLNGNVISATISNDKAFIVYTVKSPEKIIEAQKTIELYTSYIYDKRTQSSSVLENASHYPLYYHFLPGFHEKSSVQLLLFQNDECYTVKVTLKTDESQQQYVPLNKKPSKTNIFRKCFWYQYDSVNYTLYSLQTKQKSTFVSSSSGSVNGNANGANNGSALPPSESILKIWSLANGKVQSYDPIPLQLNVDPFKLSTNIPIPLGAGNSTGMDPVFQNIHIIKLPILGLVLCHQEESQQAHSIRISIYLLHLKQKIRYSIPLNAMGDGISSSTRVLFDHFGSLLIIYIPGYFFQYIDCALDHEPCIGITLFKDLSLRLTDLANNQDSAGILSARVKPLPDKLSTSIIPFSFSSADPRKEKEHHHTTGSAKSGQYLFDYANGILYHYQFTRESIRNLFEPGIMEPGTDVQAIHLAIIHLDDRELGTSLITRASQSNLFTTDLFKEYILCSTYSVLRTNNIDPALLHSLPLSYPDGIDVSQHNRRLKDLEVSLINTTIPTMFMRKEENRRRLKTFEVTKSEITNKDGPTFSGFWRNFFGIDDFADIQIGARAKEGLSNSGGKINTSNHKGQMQVEQHLFENTTYEEDPLKEINLYVISVSEFFNQISQRETKDKLVEWATAFRHIQINVVNTLYKTIKICCCGGANINDVFAIKDMRHSNRLTLFQALEKLYSAIEELYCPFPKDFYTQFTTLGFYCLQRPVFLQFLQKGIFVITKSFVQVIKKEFPNLDKLSQPTRDFINQIILQLRDQESVIEFLQSDPTNTKFIVEHLISLATQNIHPKSYVDPNSVDLMPDYSDSSFLPLSVLHDFLKSLVTNIPSHISTPISSPISTSSNGNSVGGGNNPANNNNNSKFYTPPTSPLRISSSNQSVITAYQPFSCSPVNFNNLSSSMGNLNISSPPNVSPMMSGGANGGVGGNPPQRDPMSTSLGNLNNLTSNPIYNNSQNILNAFHFLDQNTDKILQQMFENVNNAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZR5</accession>
    <name>A0A8J4UZR5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010468</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001133">
        <property type="protein sequence ID" value="KAF2068207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZR5"/>
    <dbReference type="OrthoDB" id="64113at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="155" mass="18280" checksum="4C641434A6F35F96" modified="2022-05-25" version="1">MSIEQRVKQFYDEAVKISNGTLAITKANKEITTLRKFIHNNQNHNFHEREKAKIFATIELYFSLCNRKKDISPTDTNDKPLSAQDLIEDSLKVPLFTTKQKKSMLKWLEDELLEPNVGLSKQQEKKNAEKKKYYDHDSDDDEYNYDEEDQEEDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0V6</accession>
    <name>A0A8J4V0V6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein-tyrosine-phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_008963</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Has a dual specificity toward Ser/Thr and Tyr-containing proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000607">
        <property type="protein sequence ID" value="KAF2069722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0V6"/>
    <dbReference type="OrthoDB" id="2017893at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008138">
        <property type="term" value="F:protein tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000161">
        <property type="entry name" value="Probable dual specificity protein phosphatase DDB_G0269404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016278">
        <property type="entry name" value="DUSP12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45848:SF4">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45848">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000941">
        <property type="entry name" value="DUSP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="9">
        <location>
            <begin position="3"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="10">
        <location>
            <begin position="60"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="165"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="167"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="7">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00055556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000941-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2069722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="389" mass="44276" checksum="804F00C95B42DB46" modified="2022-05-25" version="1">MDDVTRIFPGFYLGSVPAVNRSNLDDHGITHIVSVLNGFKPKWTNMYKYLHIDIIDTPSAELYQYFEKTFEFIEKGRQEGNVLVHCFAGISRSATVVVAYIMRKLKISLEDALGLVTDARPIVCPNEGFMKQLQKYEAEIKASTIKAKTTSQSKIYYYGPKNTTFDIVDDDEDEEDEDDDDFTDDDEDSEEEEKDKKKKPTTKGQATTTTTIQSLSEATVNISISDESSTNKTTETTATITTTTTTITAETRANEEEDEENENKEIIKINRELGQYRYCCRGCSKELFLDYDILDHEQGSGQTAFKWNKRDHHDQNKKDDIICTSFFLGEINFVLEQTYSGMDGKLNCDNCKEKLGSWSWSGEQCSCGAWIAPSFQIPKTRIDEKRVLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V103</accession>
    <name>A0A8J4V103_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sec1-like family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003580</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000114">
        <property type="protein sequence ID" value="KAF2075103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V103"/>
    <dbReference type="OrthoDB" id="2228at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2060:FF:000001">
        <property type="entry name" value="syntaxin-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="601" mass="68018" checksum="172FA32F9FBA820F" modified="2022-05-25" version="1">MAASLRDISRDRILNEMVRPVSPEASGGWKALVVDQDSLRIISACCGMFEIMEEKVTVVEKIDNPRQRLPNLEAIYFLTPTTKSIDLLINDFKKKSSPHYLAIHLFLTSKLPDSEFKKLSASNAVHRIKTFKEINLEYLAVESQVFHFDQKHTLHTLFSPESINPIEEQARVASRLVSLCVSLNECPIIRYSRNNTVSAVIAGLTQEKLDSVMRGVKSFRPNDDRATLLILDRSQDPLAPLLHEFTYQAMIYDLFDIQNDKFSFDSLSNAGVSVKKEVLLGETDYMWTGLRHQHIADVIDYLKNKLDEFLRTNQVSQYTQHNTGSLKEAGEVIRNLPQYQEMMGKYSVHIHLAEQASAKFTPDLENLAYLEQDMATGEDSKGSSPKNIVGRLSNMLSDFNTSKMDKLRLLMIYIISQEGIKEQDRKKLMDLAGFDQAEQSSIANLFYLGVTLMKGAKGKQKTQINKIRKGDSSDVPYEVSRYVPVLKDIVENLTNDSLPSSDFPFIKEEPIAKASNAPVSKVSLKGKSKQPRWADPNTQVEETKYSGSKLIVFVIGGMTYSEMRSIYEISSYYKKNIYLGSTGLLLPKDFVEDVQSLKKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V106</accession>
    <name>A0A8J4V106_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002144</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000057">
        <property type="protein sequence ID" value="KAF2076530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V106"/>
    <dbReference type="OrthoDB" id="20693at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38736:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38736">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="27751" checksum="749933E4261403BA" modified="2022-05-25" version="1">MSRGGRLGAALLFNALFVSCCIISFTTWWYRIQIKSSISTDTTYFSWTFSRFENGLTPGKIVESNYKDYNWDTIANVCASSLGLLTVSCAFGLLSFLLQVITLGTKNKGTKISGGIFLILAGLLMLASFFQYIRIGNGFQDDLPECKVLNLPNIIPTNSPSSSTTGSTTSPWYYDSDDYLNSPDHLDRKEVTADNIEITSGFEKLYCQSFNGHNDKLVAGTNIRWGPFVGWYILVPGFVFAVVSSLCTFLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V172</accession>
    <name>A0A8J4V172_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_001959</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000050">
        <property type="protein sequence ID" value="KAF2076770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V172"/>
    <dbReference type="OrthoDB" id="186625at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051426">
        <property type="entry name" value="Peflin/Sorcin_CaBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212:SF3">
        <property type="entry name" value="GH27120P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212">
        <property type="entry name" value="PEFLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="75"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2076770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="204" mass="23114" checksum="26432D3375317D59" modified="2022-05-25" version="1">MYTSAYAYRQPMAAVGFALAPPSAFQSCWYYSLYTQIAQQQLYEYQAWFMSMDRDRSGTLTSLELQSLVIGGFPLGLETATKLIKVFDHNRNGQIDFYEYAALHQFVNNLYRCFVVNDRNLSGTIDAYEIHNSLTSAGFVLPFHTVNLFFLKISPLGTGIIFTQYLGLCASIALARSLFEWNDPMRTGVIHIGLGQLYDMFALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V204</accession>
    <name>A0A8J4V204_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000977">
        <property type="protein sequence ID" value="KAF2068459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V204"/>
    <dbReference type="OrthoDB" id="5689at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="74"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="216" mass="24833" checksum="4C472A43DBC6C8C3" modified="2022-05-25" version="1">MISTDSCIATTQHNSLLNTKIIGKKITCKARLLDKRDFQHLIKVVNWAYRGKEGHEQWASEENLIFGDRVTYDMLLKDIEKDKNLCTVIVVEEYIDQEENGEGCDQQDKILQPIGCVKIERDTIHDKPIIGMLSVDPSYQSKGIGAILMTLAEKEIKQVWNVDEAHLHVVNVRDGLLGWYRYLGYVENGERYPFNIPGTKILVDEELEFLILIKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2G1</accession>
    <name>A0A8J4V2G1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009966</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000867">
        <property type="protein sequence ID" value="KAF2068714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2G1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="92" mass="10556" checksum="6F7C217D471AB357" modified="2022-05-25" version="1">MLFSLSDRPMQAIEDKFNAWKLLTSTLQRKIYILKTYALSTLTSKPTRIHILHMPSRKEGFNTFASTRVTKSQQPTAVKCSKDILRSLLIPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2K4</accession>
    <name>A0A8J4V2K4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009829</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000822">
        <property type="protein sequence ID" value="KAF2068849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2K4"/>
    <dbReference type="OrthoDB" id="659430at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="194" mass="21967" checksum="0F7A4504562C48CD" modified="2022-05-25" version="1">MYWYLKVEQQEKAIKIDMVPLTTGNITLKEFTDSPSKKSSRVSSFISDSKSIHLYSIYGCFNYFIVPHLLGINIKSIKFGENSVEKLVFGDKFNHKLNKSILPTSLLTLVLGRDFDQDLIDNLPSSLTELMLNTESKPSFTSSSQFPPNLKKLALPCYDGVLDIIPTTINHLELNVSIAKKTLVNKVKLYFQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2L1</accession>
    <name>A0A8J4V2L1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000004">
        <property type="protein sequence ID" value="KAF2078465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2L1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1017" mass="115911" checksum="4710AA8849431288" modified="2022-05-25" version="1">MTTTNTIASLDLFYTVYRNKYLRNLIRDNKYRGLVKEITLDQLENDHQFIASLVDSYNTDNKFNDNNTRNNNIIFKLIIFNYHQFDRFINGPHRHLINSIRINEKLSAFGVDRELSWEEAIEFDLGMIPQGIQRLDITVDESVRGTGQLPDSITELKVNQSITYRQNEVNFFDHLFANLPPNLAKLALPYELKLIQRKVVLPPSLIDLDIKAGSEILNNFVVQGLDRQFKSTIMKIDSAEDLEILENLGWINKIILGSRLGGKKVVVPHHVQHITNPYSSQPFKSITFPPNLKSLHWPKVDMVIEKGLFPDCLKSLNINLYDHPIEPAVLPNGLESLYIQCKSPLEQGSVPCKLKTLFLYNYNHALKSNVLPVGLKSLTCNTFDNVLGSNSLPTTISYLELREFKGSFELVGPLNHLSKLFIDTMDASVSILLANVKRIEIYCKKITPDCSLKHTAIKNLYLFKIGNPHTLSTDFLPSDLVKLVMSNADIKSPNVIPNGCVFLKHDLFLEPSFIPSSVKKIIRSKICRDLKIKVDLKYLDDNHGYLSLLSEKDKIDNNIFIELCILDEDELNGYYDHPHRCLIDTLDIDMLAHNMLLDSGFIHRGVRVLYFPMSEGIRFIGDLPDTITELHLGQWHNANHFLWNLPPHLTLLSIPSVYRVQRRCVMPDTLTRLDYTFPVDKETLDNIVVSPNRNRELGGAKLLVRSRDSFPLIHRYKWVTQIRVSPSLLPLEPNQIPEHIESINVKNSLYGSKQDLECLNLWNPGVLPRGLKSFSDPNFCGDLVKGLLPDSLTSLLISSFRPIQVGVFPNSITEMTLTYTFPLQAGTLPLSLTKLTLNDYNLPLCSHVMPNRLKELHIPQFNKTLEPDSLPASLTSLRLDRFTRSFKSVGPLCNLTQLHVESFNASISILLAHTKQIHLSTEYIDWSTSPSLLNTPIETLRISIKLDRTPIPMSFLPHSLVKLEINNFYINSLDVIPNSCLLLTTINDTDHVNPVFLPKSTKVYHKQIKKTNTRFTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2M8</accession>
    <name>A0A8J4V2M8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TRAF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_009758</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probable adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000797">
        <property type="protein sequence ID" value="KAF2068924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2M8"/>
    <dbReference type="OrthoDB" id="5574452at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001293">
        <property type="entry name" value="Znf_TRAF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02176">
        <property type="entry name" value="zf-TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50145">
        <property type="entry name" value="ZF_TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TRAF-type" evidence="7">
        <location>
            <begin position="2"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TRAF-type" evidence="5">
        <location>
            <begin position="2"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="220"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="250"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2068924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="260" mass="29423" checksum="E61EF7C1A7F32A43" modified="2022-05-25" version="1">MPIFCKLWCTSETTRAIMAKHIEDECPKGTIPCPFLTMGCKDKFQRSTLAAHIALYDYHSNFIQSFSSKNQQLLDQSAKLQLYINSCNKRNSDCLAINKNLQEEKVKLQDAVYARDTLIQASGNKLQIILEQHKQDTEALQSLTINSFKDKIELLHTQVEVIQGEKKVLSKNFATLQTNYLQLLNQNARLTKEGKAHEFDKACYLSELKVLQDEKKTLDDLLARYRSQPSPKPSSPSLSPPPPSSPPPHSSNSSPNCRNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V340</accession>
    <name>A0A8J4V340_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cullin family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_006478</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">Belongs to the cullin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000300">
        <property type="protein sequence ID" value="KAF2072212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V340"/>
    <dbReference type="OrthoDB" id="27073at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031461">
        <property type="term" value="C:cullin-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000014">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000007">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000019">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.130:FF:000003">
        <property type="entry name" value="Cullin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000001">
        <property type="entry name" value="Cullin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016157">
        <property type="entry name" value="Cullin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10557">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01256">
        <property type="entry name" value="CULLIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cullin family profile" evidence="8">
        <location>
            <begin position="381"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2072212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="736" mass="85893" checksum="25549FFECE0AC45E" modified="2022-05-25" version="1">MSSGGLVRKIIDFNETWDSISKGVLNLLTGVQNVSAMYLFEDVYSLCVAQPHPYCEPLYENVKKFFEDHVDRLLQVILDTKSDTISEYLKQWKQFSDGCQLCNTVVFRYLNNNWISKKISDKKYGHPPDIYEIQTLGLMIWKERLFFQIIGRVLKCVEILIQKDRDGELVQHSFITQFMESLIKLDSVDKDKSLYTNEYESLYLENTKQFYTRESVAFISSSGISSYMKKAEERIEEEYHRSQKYLAPSSHDKLRRLLDSILIEKHKELLQSECINYLKDEKLDEIHHMYKLLSRIESGLTPVLETVQNYIQHVGFEAIKAIPDRNNPDPKNYVETLLKIYLQFSSIIKKSFNNDVSFITVLDSACHKIFNDNHITKNTTKSPELLAKYCDILLKKGNKQHEEIELEEKLGQIIVLFKYVDDKDVFQKFYSKMLSRRLINGTSVSDDIEKLMITGLKQACGFEYTSKFTRMFTDITLSTETNERFKTHLQTNNISIGKIDFSILVLTSGSWSLHSQTSSFIVPQELTVCISAFQSYYQNHHQGRKLNWLHHLCKAEVKSFFAKKTYDFQVVNYQLGILLLFNNQDSITLDDITKFTNLNEQELPRTLQSLVEAKIITQKKNPDSATQEYILNTSYTNKRLKVKVSSSLQKETQSQNEETYKGVDEDRKLYLQASIVRIMKARKNMNHLSLIQEVIEHSKARFQPNIPMIKKCIEQLIEKEYIQRAEGESDKYLYVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3J4</accession>
    <name>A0A8J4V3J4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UPF3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_001840</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000046">
        <property type="protein sequence ID" value="KAF2076873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3J4"/>
    <dbReference type="OrthoDB" id="18087at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039722">
        <property type="entry name" value="Upf3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005120">
        <property type="entry name" value="UPF3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13112:SF0">
        <property type="entry name" value="FI21285P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13112">
        <property type="entry name" value="UPF3 REGULATOR OF NONSENSE TRANSCRIPTS-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03467">
        <property type="entry name" value="Smg4_UPF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF3" evidence="4">
        <location>
            <begin position="6"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="156"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="156"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="193"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="233"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="252"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="274"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="312"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="364"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2076873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="374" mass="41145" checksum="F455C19B594EE039" modified="2022-05-25" version="1">MEVYFVIRNLPYNLEKEKLTSIIYSAFSAETVQIVDYIQGSKSKKYKDQVTATRCYVKVNNRLGTKSSDAFIDFMDLKPFINVRGLEELAMVESVPYTDEGLATPNKSAGTIESSKSYQSFLKQLNTPVVIKPYVPDDSKKQQDVSVIIEEYLSKKKKKRNNRKKSKKAEARGTKANNGVGGSAGATQTPSGGRKKKNRSQKRKEKQQALKLQGVTIAKNPNKPVKDQQQQDSVPSQTTTTPATASVVKDNTANKEKQQQRKEKQKKAKEAKKLAQQQAQQTGTSTPTTTTAPATTQPKEKKPKPAPKDKPQPTTTTTAASSTTATSTTTAPAATQVKEKKPRPPRTERKTTAVRYEVKNPSPAAAPPVETPKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3P0</accession>
    <name>A0A8J4V3P0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_008691</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000561">
        <property type="protein sequence ID" value="KAF2069989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3P0"/>
    <dbReference type="OrthoDB" id="29755at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="4">
        <location>
            <begin position="171"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="134"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="209"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2069989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="265" mass="30568" checksum="88DEA9C70179B11D" modified="2022-05-25" version="1">MNSLLKRVDKIKKETVDANDKNKQLNADGTTDAFTLLRRKIGLDIKGIRELIKERDEAEATMPGSVRTVDLSHRIRVAIQEIKTETQKLQRMYEKAQDRYNKKNKENPEKLKDIELQKESCELAWAHIKELENQNRKRGGETSNFIPRDHQSSSTVKQLPDIDNDDFKELLRNDKQIDNVLDKIQENLKVTENIANEMGKTAKRQGVMLDNLNDKADDLDTKLENINIRLAKMIKDVRKADRFIIDVILICVLLGIGGFIYSLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6I2</accession>
    <name>A0A8J4V6I2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_002843</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000083">
        <property type="protein sequence ID" value="KAF2075857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6I2"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000966">
        <property type="entry name" value="Serine/threonine-protein kinase 3, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF2">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="21"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="368"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="319"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="368"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="409"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="425"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="454"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="481"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="535"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2075857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="552" mass="60939" checksum="E2067D07C1D14358" modified="2022-05-25" version="1">MDNVEQLFIDRISKDDPEAIFQIVEVVGSGSFGTVCACRWMKKKDREQNGNRLIACKFVEVNPEDTETNNNLVKEIDILRESMDCPYIVEYKGCYLKSSMLLIVMEYCKGGSLLDIIEFCGKRLIEDEIAAVCAGVVKGLVYLHSKRTTHRDIKAGNVLLDEDGVPKLADFGVSTIAEQGQKMKTVIGSPYWMAPEIIMGQGYDQKADIWSLGITAIEIAELIPPRFDIPPSRVIFTIPHQPPPTLKTPSDWSPEFVDFVKLCLSMNPASRPSAQQLLSHPFILKGSSQQILQKLVNECIPLLKEKRAEKIRKMEEQAATEQQRNSGSKMGNLPPTRASVAYASIQRAESLKGSVVILNSNTKTASILRNKNPAPASTSSDANGTARKLPTTKPPPNNKSVLNRYPPANNVNSNTQAPTPVGGTNRPNKVQSPFLQNQNGAQQTPTPPPKPASKKQPVATTTTTASNNSNNTSNLTSSNNQVKPNSNVSRSTISIGQQKSGNSFIRASPQQLQQHQQDEYDEEDSRSVIYHPSDSEDEEDFNKEDYEEISVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6T9</accession>
    <name>A0A8J4V6T9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2</fullName>
            <shortName evidence="8">Oligosaccharyl transferase subunit OST2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_002525</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Endoplasmic reticulum membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the DAD/OST2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000070">
        <property type="protein sequence ID" value="KAF2076172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6T9"/>
    <dbReference type="OrthoDB" id="445566at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003038">
        <property type="entry name" value="DAD/Ost2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10705">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10705:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02109">
        <property type="entry name" value="DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005588">
        <property type="entry name" value="DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2076172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="122" mass="13600" checksum="C54504219F2D8DC5" modified="2022-05-25" version="1">MSSTTATKNRGDNLTLSSIISSFSDSYSKTPQKLKIIDLFLIYTFLTGVFVFGYCCLVGTFPFNSFLASFISCVGCFILTVCLRIQINPINNFGKTISVERAFADYLFCNLILHLVVFNFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6W8</accession>
    <name>A0A8J4V6W8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005381</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000213">
        <property type="protein sequence ID" value="KAF2073304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6W8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="96"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="15988" checksum="4665738C7FB119B0" modified="2022-05-25" version="1">MDKSLFVVALIYFIFVSVATFGKSINSILSSNYLFITYFFGILVFLFPFEWKNIQNYFHPTTQQETTPSTTNNLSSNQESISAIDIDIDDGEDTNNEQTTNNNNTNYKQSLSLSVSIEIPTTKGDEELSFPHNHSPIPIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7S6</accession>
    <name>A0A8J4V7S6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ARF-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_004412</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000155">
        <property type="protein sequence ID" value="KAF2074279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7S6"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04153">
        <property type="entry name" value="Arl5_Arl8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000728">
        <property type="entry name" value="ADP-ribosylation factor-like protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="27"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="129"/>
            <end position="132"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2074279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="20759" checksum="B264EC4DF889CF62" modified="2022-05-25" version="1">MGSSLSVFAKIWNRFFNNAEYKVIIVGLNAAGKTTTLYKLLLDEVVSTTPTVGSNLEEFVYRNIRLLMWDLGGQDLLRSTWNQYYINTQAVILVIDSTDRVRVQLIKEELFKMLAHENLKKAVILIYANKQDLQDAMSPTELTNLLSLHSIKDHDYHIQACCALTGQGLEPGLDWLVSHITKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7V3</accession>
    <name>A0A8J4V7V3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004330</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000151">
        <property type="protein sequence ID" value="KAF2074354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7V3"/>
    <dbReference type="OrthoDB" id="10253401at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10245:SF15">
        <property type="entry name" value="ENDOTHELIAL DIFFERENTIATION-RELATED FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10245">
        <property type="entry name" value="ENDOTHELIAL DIFFERENTIATION-RELATED FACTOR 1 MULTIPROTEIN BRIDGING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="2">
        <location>
            <begin position="43"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="104" mass="11269" checksum="EE28040919224E71" modified="2022-05-25" version="1">MADIVKKYNAGTNKVATGINAKKIEESEDVKIPELKVSVSRAIQKARVAMGINQKELAIKINERQGVVNDYENGNAIPSQAVLGKLEKALNVKLRGKDIGSPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V816</accession>
    <name>A0A8J4V816_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001300</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000030">
        <property type="protein sequence ID" value="KAF2077372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V816"/>
    <dbReference type="OrthoDB" id="10264655at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21797">
        <property type="entry name" value="CycT2-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036580">
        <property type="entry name" value="Cyclin_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0195">Cyclin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="3">
        <location>
            <begin position="36"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="3">
        <location>
            <begin position="149"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="239"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="239"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="296"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="315"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="328"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="338" mass="38160" checksum="1B72FB9E9279F2F9" modified="2022-05-25" version="1">MSTKTNSKWYFTKEQVLKHYGPDVKFEVTYRRASAAFIQEMGIKLKLPQLTIATAIAYFHRFFIRHQLKDHDRYIVSTASLFLAGKVEETPRKLDDVIKVSNMIRHKKKPENTPTSPEELAEIKNQVLTSEHAILTTIAFELAVDHPYKYLLEFMKAIGGNISLCQVAWNFVNDSLRTSLCLNYPPDLISYASIYLATKFLHYHLYTEGKKQQWWEVLNIKLEVLEDISKQILDLYEPSQLPPGAQSSSSSSSSSTTNNNNNQSATTTPSNSTSNGRSSSPLPPPPPPPNDREEGTNVNTTPTSSSNRLHPGSAGKNQRYSPYGNSPYRKEKESNNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8E2</accession>
    <name>A0A8J4V8E2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MACPF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_000979</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000021">
        <property type="protein sequence ID" value="KAF2077732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8E2"/>
    <dbReference type="OrthoDB" id="21110at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022829">
        <property type="term" value="F:wide pore channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052784">
        <property type="entry name" value="Perforin-1_pore-forming"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46096">
        <property type="entry name" value="PERFORIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46096:SF3">
        <property type="entry name" value="PERFORIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00457">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035275791" description="MACPF domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="domain" description="MACPF" evidence="4">
        <location>
            <begin position="491"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="729"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="744"/>
            <end position="759"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2077732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1128" mass="125641" checksum="4387813C5F4F2FB7" modified="2022-05-25" version="1">MRFLLFLSLLISVCLEASSTCVSNCGSHENPFKTFKEAISEISKYKLESGSIPYLIVKQGDYFGIENKGINITIDINIVSTKGSSETTLDCQNAGKAFNVEGPISFSLTGFTIKGCTSGRGGAIYSKNLMTNIEDIAFIENNARLGSAIYSTSSVFNIEACMFYNNNGGHAVHFVGVSSDISNSQFNMNSNDVFCENGNIVSSNAKYGSICVDCSILSAQTDTDICGSSKSSIQCNSDGICQKWIENYSNCPSDCPYLHGCIPNGICNEKDGETFTNCPMDCDPKNQTGWILDSYDYSLSKPIKSMAYFADDSKKIQTTFIPFSDVRSFMAKKYDPVSGRLTTNVIVPKDGDFFFRLTTENIGAIVFVNGRVLFDTFFPFNIQKEFNSERKMLMVQNVPTFVEIVFISHSSNERNIALEWRHETESEYQPIPSTFISYDKEFTCGDNVCNEIKPETCLIDCYAHIEKKCPGQSPPAKLQDFYGNTDSDIVGKLLNNQYIFSLPGMSYMSHAIDIRTGVPNTSPLFDLSYCDNTSFSIVQDPYRGLVYSVPKGLFAQASPKCTMDSTTKTYSNSAHMSKEESQDMSLDVSASGGGGCAFVQVSVSASLSLSQSTKSAKDTETKKDGSLSKTTISCETTKVHLVEPRFHPKFIEDVSNCYVKNNKNKTNENLQKVIQKYGSTYMTHATMGGKLEVITSVDHSFSSNKTSSEVEKAMEMSVSAQVSSKVLSGSASATLGRDSKTTSEEQSTFESNSERSTVQIYGGEPGSYGMNEPNAFATWAKTVDLVPYPIKYKAGYVADILPDSWFFEDGHSVKLLWLINEWKMFKSYLQTRNFKYIDYDQIITQEEELQEQHLQRNDTTYHLNCSLLANFDLEITNSKYKTFYYSNVPSFDGSNPRSNIVISYNDTKGIFSMAFTTKTGASLGIKNVTSPDGSIIYYDIGQSFDIYNIFSSRYYHMVLHPNGTYIISNTINPKSLVFKFNQLTITRQQVSGGVGEGSGTINGKMVVMVYGSLEKEKIYIPLTSTPEQTYTFRTNNDYIGQVLGLSFRYETDQNDPYEKNILMKYESLIVLQACGKESSHCIPKNNYFDENLGLVLSYESWRKETGQSLITVPHENTAQWETLEQLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8R4</accession>
    <name>A0A8J4V8R4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Actin bundling protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_003358</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000104">
        <property type="protein sequence ID" value="KAF2075329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8R4"/>
    <dbReference type="OrthoDB" id="431378at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032432">
        <property type="term" value="C:actin filament bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051639">
        <property type="term" value="P:actin filament network formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21295">
        <property type="entry name" value="CH_PLS_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21298">
        <property type="entry name" value="CH_PLS_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21301">
        <property type="entry name" value="CH_PLS_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000042">
        <property type="entry name" value="Fimbrin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000263">
        <property type="entry name" value="plastin-1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000010">
        <property type="entry name" value="Plastin-3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000016">
        <property type="entry name" value="Probable fimbrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039959">
        <property type="entry name" value="Fimbrin/Plastin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961:SF18">
        <property type="entry name" value="FI19014P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961">
        <property type="entry name" value="FIMBRIN/PLASTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="7"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="43"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="5">
        <location>
            <begin position="116"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="5">
        <location>
            <begin position="260"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="5">
        <location>
            <begin position="380"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="5">
        <location>
            <begin position="502"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="608" mass="67025" checksum="FD1B05C1B8A5C72B" modified="2022-05-25" version="1">MSGFNESDLVEFRKQFKQFDENGDGSISSIELQKVMTALGEKVTGLEVREMIKEVDTNGDGSIDFNEFVQVMAISKKLSANNSPAFASVVKKHNQVNTIGGYSGSTASGVQHSYSDEEKVAYIDWINNCLAKDSDLKGKLPISEEGDAFFKACHDGLLLCKLINDAVPETIDERVLNKKNLNTFRINENQVLCVNSAKAIGCNIVNIGATDLMEGRAHLIMGLTWQIIKIGLFARINLTNHPELYRLLQDGETIEDLLKLPVEEILLRWFNYHLAAAGHPRRVKNFGSDIKDSENYTVLLKQIAPANAGVDTRALNESSVDKRAAIVLENADKLGCKKFLKPRDIVNGNTKLNMAFVANLFNTHPALAPLEKPIEIIQETREEKTFRNWMNSLGVDPFVNHLYEGVCDGLALIQLFDKIYPGLVDHKRVNYPPYKAMGAEMKKIENCNYAVELGKNVKYSLVGIDGKNVYDKNKTPVLSILWQLMRGNLLAILNSLSKDGKPIADAEILAWTNSKLSGKKISSFKDGSLSNAVPILSVIEAITPGSIDQSLVATGTSEEESLLNAKLAISTGRKIGATIFALPEDIYEVKPKMILALFAGLMAVEMGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PK56</accession>
    <name>A0A8J4PK56_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CCZ1/INTU/HSP4 first Longin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_009458</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCZ1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000714">
        <property type="protein sequence ID" value="KAF2069222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PK56"/>
    <dbReference type="OrthoDB" id="240546at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035658">
        <property type="term" value="C:Mon1-Ccz1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013176">
        <property type="entry name" value="Ccz1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043987">
        <property type="entry name" value="CCZ1/INTU/HSP4_longin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043989">
        <property type="entry name" value="CCZ1/INTU/HSP4_longin_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13056">
        <property type="entry name" value="VACUOLAR FUSION PROTEIN CCZ1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13056:SF0">
        <property type="entry name" value="VACUOLAR FUSION PROTEIN CCZ1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19031">
        <property type="entry name" value="Intu_longin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19033">
        <property type="entry name" value="Intu_longin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCZ1/INTU/HSP4 first Longin" evidence="2">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="CCZ1/INTU/HPS4 third Longin" evidence="3">
        <location>
            <begin position="335"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2069222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="435" mass="50352" checksum="D1BB2B4F91AE6B72" modified="2022-05-25" version="1" fragment="single">QREGSEHEKILFCYPPTISIGEQTNYVGLTEGYILCSKQFSPNQPCEAIHTQKTTIGIYSPENDIYMLLSLNNPAGPLGKDGKREYYEDEIDDLLVQKLLQQTYQTWLLFNGTMNSTILRTGNLGGYEYLRKRLDSFLKPYVKMMPLDQLDLFNALGGIKFLPIDKNIYLTIFNYVNSAEVSFQTTLSTFRFGVLLYKDNLILSSMDQNETRLLYNYLVNQVKVGQDSAYCLPLMASNVDNENQWYTKGTSRRTGFLNTPDNVSKRDLPIIYLENGTKPTYMIIYEQKDTISLFLIDPSELDQLLLKELSSCLVSNFEFINPALEDGYNKKTAFDEQYKYIYFNQMNLAIKASIKSKGAELTKETIKMLNEIHSDFEKKLSSEVTIRTQSDRWIVAKKVDYREFYVIFDNKNINILEVTEEVKNLTSKYFKIDHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL03</accession>
    <name>A0A8J4PL03_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009842</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000826">
        <property type="protein sequence ID" value="KAF2068838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL03"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF29">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="105" mass="11434" checksum="F8D21EDBE552420B" modified="2022-05-25" version="1" fragment="single">NQYNSISTQSEQQTYIGINIPFYSKSVQLDPDFSVLIDTRPASDTQDAICGGKSKSKLTKPQLIGIIIGGVAFAAVLVVGLTYIVYKKRQKSKFEASLSSKLSQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL80</accession>
    <name>A0A8J4PL80_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA (guanosine(18)-2'-O)-methyltransferase TARBP1</fullName>
            <ecNumber evidence="8">2.1.1.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">TAR RNA-binding protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_008683</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">S-adenosyl-L-methionine-dependent 2'-O-ribose methyltransferase that catalyzes the formation of 2'-O-methylguanosine at position 18 (Gm18) in a subset of tRNA. Selectively mediates Gm18 methylation of tRNAGln-TTG/CTG and tRNASer-TGA/GCT. Gm18 modification can enhance the stability of modified tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>guanosine(18) in tRNA + S-adenosyl-L-methionine = 2'-O-methylguanosine(18) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20077"/>
            <dbReference type="Rhea" id="RHEA-COMP:10190"/>
            <dbReference type="Rhea" id="RHEA-COMP:10192"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74445"/>
            <dbReference type="EC" id="2.1.1.34"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:20078"/>
        </physiologicalReaction>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.34" evidence="8"/>
    <dbReference type="EMBL" id="AJWJ01000559">
        <property type="protein sequence ID" value="KAF2070000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="241340at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141100">
        <property type="term" value="F:tRNA (guanine(18)-2'-O)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18091">
        <property type="entry name" value="SpoU-like_TRM3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.10:FF:000010">
        <property type="entry name" value="probable methyltransferase TARBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045330">
        <property type="entry name" value="TRM3/TARBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044748">
        <property type="entry name" value="Trm3/TARBP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12029">
        <property type="entry name" value="RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12029:SF49">
        <property type="entry name" value="TRNA_RRNA METHYLTRANSFERASE SPOU FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="tRNA/rRNA methyltransferase SpoU type" evidence="12">
        <location>
            <begin position="1330"/>
            <end position="1471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1197"/>
            <end position="1227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00093266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00093594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00093636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00093656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2070000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1486" mass="173383" checksum="561A55BF54A80DB1" modified="2022-05-25" version="1">MNFNLIQFLKDIYSSTNQINGTKSLVNSIFYLVPNIEKHQLLADVKQYILTTTTTTTTNNSDNQFILFLLSIIYDIIDYQKQESTSLNEIINEILKIYINYFINSNQQNEMNQLSNIISKIIEKVSSVEIFNTFIDLIKYQLENTSNNNKLGYYIIHQIVNNDISSDDSSIISTKFYSILVPVLIDNLENYTIIGQHSKQQSLIILSKIMLDKGVLGKEIELQLEQQYNQEWLPNLWRAITTNSKDIYEKLKLAFFFHGCYFDCIVKYFKKKEPIPFNLLAQPQYWEILYQSLIDKDSLPRKQGIHLLKNTISLAQPIWKQFLPQNWSTKQWNLFYSLYESFDETKSHLFIPVWVQMNNLLNDSQDSFTNLSNPKSIYFWIGILFLRGFNHVNINIRKRVIADCLSSDFISNNLISIPFDKFSSQIINSFDSLEISTIYENENKLLLKFLSNIFKNNSNIEYLEILIQYLATNVGETKTIVIFLNCLKPILFGYKITTSILESCRNLLNTHPIAMAPINVVLSESVLDFLLAFEMDQSLNISFILLSKIFSSLSKYLVQQKKNLIQEWLPKSNWIINQVVSDFNQLVIGQEENYETLELSIHSVIRALGLCENDQVLGFCKNQIKNCNNEKVYTLSVQIIFQEFNNQIELLDQIYNEIYNYQFIINSIKNYINNQEENFDQILNQFKFIESISKLSTFSKIQDLQFIQYIENLILNNQSFDLKKLSIILLVYHLNNNIKFDNDNQILESLLKLEPKYKFDSTISFILPKFIQFKWLSISNLLQRQEKEKIETENLKIIFKECINNLDNATGFVLVPIFECLKICIDNNVMEDMENMFKQIYLSYSDSSRKPISLTKSFINLLFHPTLFKTTESIPLVKEYLEKTLNDWGESVRISCGLLGHCCAIWASNPECLDEFINEIIDLCLLTSEDRLVIEDEEDQNHTLFNNHGYIIRWFVSQLEGEKTKSFVEKLMIELLELNFKPEFSKREYSQQSKTNKMKCKLWRTLCSLTHIIDLNQGSMEFYSKVNNTMWKTLELKNHGNVRYLIQLFIINILIKASQSQIDNNGKELIDRIENAINSDYQMSASLIIISSSYLYYLLNKQFSNQSLILNLFKMIIPWTTDFHHAVRTTAQLAIYSILSKKYEILKEFNDNDMLKSMYHYLDTHKIQKRLREKQTLFIQNDDPLFKTLAENILTSNTNTIQDENEEEENNGPVGNNNDDDDDFGQLSVNQDESIIPMSLLDISKRLIKDFQVSFQNNIINHETKNPPNTIAEKVLIQENNTDDDSDILDFQKRITPWQWVQKLEEHHQINNNNSNNNNNNKNNSKRQSMIIVGTFIENTPNLAGLIRTCEIFNVEQVAIPNIKLLNDPQFQRVSVSAEKWVPTIEVTKPNLISYLTLKKKEGYSILGVEQTSQSKNLSTFEFPKKCVLLLGQEQNGIPAEYLNIVDYCIEIPQLGQIRSLNVHVSASIMIWEYTQQQILASNNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLU7</accession>
    <name>A0A8J4PLU7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009364</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000689">
        <property type="protein sequence ID" value="KAF2069318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLU7"/>
    <dbReference type="OrthoDB" id="2161881at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39408">
        <property type="entry name" value="MUCIN-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39408:SF3">
        <property type="entry name" value="MUCIN-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="954"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="156"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="954"/>
            <end position="1002"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1016" mass="117690" checksum="72B017E469357D67" modified="2022-05-25" version="1">MNNNEIDFDFGSDDANDFDFGSNDNTINNNNNNNNNNVYMISQQDFNKISSSSTTNTNINTSNNNSSSSSSNSSNISDSKSTLYKLPSDLSPSTLLPSCSLSSSLIYNHIINDLTTTPTVTTTGSRAEKKTKPKLFIHNEINDLKQFIIDNHLKKNSNNNTDTAPINNNNNNNNNNNNNNNNNNNNNNNNNNNDNDYSIPNNKFYQKKTTFSIYKDIVQNNIKSKLLLILPPCLSYISIVLLYCLHFIKKDEPIIWIKRDDITAIESDFIKRVFSLESIENNQYYIINDNNNDKKKEINNNNNSNLKFIYITEKSLNRFLKDNNLELNNFNHIVYDCFFHTIGENESFLCKPFQNSTFLLVSNSHPSNYSIIFKKYQIEKAFIYQERDFELYTMKEFIDLSSSNSFLTVVNNMISFFKNLSNTLYTNQIFSFPLNLNTFLLKPKIKSNNHNNNNLKEFEIIRNTLLILLNIGPVESFKYLYYTGYNNLSFEFQSLKNGLKILGSEVEAGIVQDNIKFEKLYQILNYLIQQFFFKKDNIKKDIQFFKILVIVKEVLSIDKVYSIIHKIQGLKVFPFVSFSQNYSLESILKDNNCIILSIQSIQDLKSQINYFSILILFDIIEQSQELILSTINCNDNCKVIKFIPCIQSKPLEMFGENDFNEQVCLLRKLSNQISNNQKDNQQTLNTFIPIYLFAGNNFLRYQEILDCCLSFYNIMFYERTLRYNIDSIFDEKNCLFYLKYPFDFKSFIQNSLLPATIEFSKCWIVIQVVEKSQMSCLNEIYQISDFQLISLKIKPFISFTSKNSSFLIRSICENIATNTSSLVEWAVQLGEVESNHEAFLSSIPGFNPYSAQMLLTWRPLQYILNASYSTLEKEFPWIPQYSWTLMSLFLDVKDKDIDKVSKKPKLIISDSKNNVDINNYSKRIQVGNNNTKLDNSLVKKNVVPKLKLPSLKINKIDNNNNNNNNNINNNNNNNIKTNNSNNNKSGLNLNNNNSNDNNINKKPSLKLQPLKFKINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLW6</accession>
    <name>A0A8J4PLW6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nucleoporin NSP1-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_008289</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleoporin NSP1/NUP62 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000498">
        <property type="protein sequence ID" value="KAF2070390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLW6"/>
    <dbReference type="OrthoDB" id="344345at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044613">
        <property type="term" value="C:nuclear pore central transport channel"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026010">
        <property type="entry name" value="NSP1/NUP62"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025574">
        <property type="entry name" value="Nucleoporin_FG_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007758">
        <property type="entry name" value="Nucleoporin_NSP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12084:SF0">
        <property type="entry name" value="NUCLEAR PORE GLYCOPROTEIN P62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12084">
        <property type="entry name" value="NUCLEAR PORE GLYCOPROTEIN P62-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05064">
        <property type="entry name" value="Nsp1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13634">
        <property type="entry name" value="Nucleoporin_FG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin NSP1-like C-terminal" evidence="10">
        <location>
            <begin position="554"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="237"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="337"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="477"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="494"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="523"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2070390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="741" mass="71698" checksum="2E869117E3CD6877" modified="2022-05-25" version="1">MFGNNTGGGLFGSAGAPSTPATGGGLFGATTTPSTGGGLFGATTPATGGGLFGASTPATGGGLFGATAAPSTSGGGLFGATATTTPSTGGGLFSTPATTTPSTGGGLFGATAAPATGGGGLFGAPASTPSTGGGLFGAPASTPATGGGLFGATTTSSTTTTPAAGGLFGSTTSSTTTTPATGGLFGATTTPSSTSTTPAAGGLFGATTTTSSTSTAPAAGGLFGSAAAPSSGGLFGTTTTTSSTSTTPATGGLFGSTTSSTTTTPATGGLFGATPSSTSTAPAAGGLFGGATTTPTTTSTTTPAAGGLFGTTIGSTTTAPTTTSAAGGLFGTTLGSTTTAPSSTATPSSGGLFGTTTPSTSSTSTTTPAAGGLFGTTLGSTTSTSTTTPTSTTTPAAGGLFGTTLGSTTTAPTTTTATPAAGGLFGTTLGSTTTASTTTPAATAGGLFGSTTGASTTTTTPAATGGLFGSTTAASTTTTTPAATGGLFGGSSLPATTATTTTPKPDSTKPNPLTTSMLGGPSGTATTTPSNNTSTALSTNIKATSSADIIEINESKVLETLLNEWISEIENQTREFYDQAKQIFNWDTQIMENSQRLNRLMEAVQSVHSQQKALDQCINVVKDQQSLLERSLNALDDKVKSSAKENETSADLERERMYYLTKKIDGDISKVENKLLGVVSKINQSQTKEDNPKDLNQKISKVLNDHLNALQWIDKSTTILQESIKTIESDANALPETKRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM17</accession>
    <name>A0A8J4PM17_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GTPase-activating Rap/Ran-GAP domain-like protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_010012</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the GARNL3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000885">
        <property type="protein sequence ID" value="KAF2068661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM17"/>
    <dbReference type="OrthoDB" id="2499658at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11210:FF:000006">
        <property type="entry name" value="GTPase-activating Rap/Ran-GAP domain-like protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11210">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035974">
        <property type="entry name" value="Rap/Ran-GAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000331">
        <property type="entry name" value="Rap/Ran_GAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050989">
        <property type="entry name" value="Rap1_Ran_GAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15711">
        <property type="entry name" value="RAP GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15711:SF65">
        <property type="entry name" value="RAPGAP_RANGAP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02145">
        <property type="entry name" value="Rap_GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111347">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50085">
        <property type="entry name" value="RAPGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rap-GAP" evidence="4">
        <location>
            <begin position="139"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00060925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00069072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="363" mass="41086" checksum="D85E293F3FB19309" modified="2022-05-25" version="1">MIASAVGYNIEGSNADSPQVVYDCNIPNLLDDVFDLSDNDSIFYRDHFLNKEHYNFLSGPSPANGPVGISIVEDSKNFIALVRSQTGNQRITIPIENVKASWWRRLFRTGPSPYDILKALGNQYNGLKLITDPRLPQAFMNLEEKQTIKGFKFGILYAQEGQNKEDEMFSNVQTSPEFEEFLDFIGERVPLNGWGNFRAGLDVRTGTTGSHSIYQRWNNNEVMYHVSCMLPFNEKDKQQLERKRHIGNDIVVIVFQDGDTVYRPTTISSRQVHVVLLVKAVTVESDPGQRYYKMAIVSKDSVPNFGPAMPQNGLFKKDQAFKDFFYAKLLNAEKASYSAPILGVKLSRTRVALLKDVSEAFVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN34</accession>
    <name>A0A8J4PN34_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">3'-5' exonuclease</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Werner Syndrome-like exonuclease</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_008636</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000552">
        <property type="protein sequence ID" value="KAF2070040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN34"/>
    <dbReference type="OrthoDB" id="1920326at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051132">
        <property type="entry name" value="3-5_Exonuclease_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF109">
        <property type="entry name" value="3'-5' EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620">
        <property type="entry name" value="3-5 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="11">
        <location>
            <begin position="230"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="845"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2070040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="873" mass="100889" checksum="C04615F3A9EBE869" modified="2022-05-25" version="1">MIRTILKCRGINANRYLVPTTRIGCQRSLFNSNQLSLNNNSSNNRFYSTDNSNNNDSNDNIKQKKPITPPEATFKSKYEIIETTERAAERSYRNCISVGQFDAKTTINIDDILDSVPKKLLENIKIHRYLLYQSVVYYLSFDTPQQTIDFYLAAREFVEKKKIVVLAKDFIRYQKPSFDPQFGITEEEMALLPQCEVPSNITIVSEIPEIETGVRLLLNGKLPSEVTKEDNFVVGFDCEWPGLKKYLLKEDPMISLIQLSNGEHTVLFRISHTGLDGTDYLSQLLMSKDIVKSGHRISTDANRLKDEYDIDMDNIFDMKFSTIAQRSTSSSYPIMVAIFLKKNFKALKTNSISNWATSGPLSQKQIETAAIKAKCNYEFYNIYKTTKVDTDVLPDDSDRQYTIIEKEGNSKLHVKEYILFFKIWSNRYIKVNLRVENTDYFRNLKRNADNQSNQQARYVDLYKDLNIFFIIYNIDQRPSLNECEWIKNIYLYSRQGSSITMIGVSSDSSKVEYTPSYHYAGYIEDDIPIVEHRLSPDMFAKAFPKIPYYEYLSYLDNTHNIYLLSELHSLIENILIEYLHDQYIFSYDTEDVVPPPPKGEELYLVCKGIKFEHPNDQDQYETNFFLVFRNKLLFNQIFESVNWIHRKHNIKYFKLGNLPIRVLVANRQFSLLPYISDQCLSPKDCTALLQCSNQIQDLQLFETLFQKNINAFESIPTQPIFKTSNSGKTTLKPLLSFMENVTIGGNLSIIQFILNNSSLKASTIKITFSCLYYSVGLGYTDIVKYYFSIPGTSFLLNNAHSGNCHFTSLLPPEYKSLSPLDILLSLASKHNHLDIHQYLSSMKSTANKDDSSKNNNNNKSKKESLFKKLFSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN36</accession>
    <name>A0A8J4PN36_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">116 kDa U5 small nuclear ribonucleoprotein component</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">U5 snRNP-specific protein, 116 kDa</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_007808</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Required for pre-mRNA splicing as component of the spliceosome, including pre-catalytic, catalytic and post-catalytic spliceosomal complexes. Component of the U5 snRNP and the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000437">
        <property type="protein sequence ID" value="KAF2070872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN36"/>
    <dbReference type="OrthoDB" id="364892at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030623">
        <property type="term" value="F:U5 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04098">
        <property type="entry name" value="eEF2_C_snRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04090">
        <property type="entry name" value="EF2_II_snRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01683">
        <property type="entry name" value="EF2_IV_snRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16264">
        <property type="entry name" value="snRNP_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04167">
        <property type="entry name" value="Snu114p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000004">
        <property type="entry name" value="116 kDa U5 small nuclear ribonucleoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000029">
        <property type="entry name" value="116 kDa U5 small nuclear ribonucleoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000009">
        <property type="entry name" value="116 kDa U5 small nuclear ribonucleoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1430.10:FF:000001">
        <property type="entry name" value="116 kDa U5 small nuclear ribonucleoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000002">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000646">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1430.10">
        <property type="entry name" value="Yeast translation eEF2 (G' domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031950">
        <property type="entry name" value="EFTUD2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044121">
        <property type="entry name" value="Snu114_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035655">
        <property type="entry name" value="U5-116kDa_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF6">
        <property type="entry name" value="116 KDA U5 SMALL NUCLEAR RIBONUCLEOPROTEIN COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16004">
        <property type="entry name" value="EFTUD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Tr-type G" evidence="12">
        <location>
            <begin position="137"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2070872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="992" mass="111276" checksum="93E81805F835B37D" modified="2022-05-25" version="1">MADTENLYDEFGNYIGEDLDEIEDEDQQQQVDDQQQQQQEDTHTNGHEDQPTIEEDVEMQPISTSSAIVLHEDKKYFPDAEEVFKGAEVMVQDEDTQPLTQPIINPKKHKEFTIVEKSYPPTTYPKQFLLDLLRFPQLSRNVCLLGSLHHGKTTFMDMLFLQTHEKKWQSSKPIKYTDTRKDEQEREISIKSTPMSLVLQNSNDKSYLINILDTPGHPNFLDEVTASIRLCDGAVIVVDALEGVMMQTERLIKHAVSEGLEICVVINKIDRLILELKLPPVDAYFKIKHTIDEINVILDIYSHTDSSSSTTGSYKVSPELGNVLFASSEMGWCFTLQSFAKIYSESFGGGFNVGEFAKRLWGDLYYDTNTRGFKRKPPTEDSVRSFVHFILNPLYKIISTVMTQEKSTTIAVLGSLGIQLSKETYDLDIRPLLRIVLSNFFGKANAFVDMLVALPSPVSAAQKKIESTYTGPLNGEYGKALLACDPQGPLMISVTKLLSKSDGSGFECLGRVLSGTLKKGQEVRVLGEKYSPDNDEDMAVEQVAGVYIGEARYKIEIETAYPGNWVLIDGIDPSIVKTATITDAQDVDPNHDHDRAHTFKPLMFITKSVCKVAIEPLNPSELPKMLDGLRKVNKSYPLLVTKAEESGEHVILGTGELYLDCVLHDLRFMYTEIEIKVDDPVISLAETIVETSSIKCHADTQNKKNRLSMIAEPLEKGLSHDIENGSIKIDWPKKKRSDFLQHEYNWDILAANSLWAFGPDSNGPNVLLNDVLPDQVNKSLLIGVSDSIIRGFQWATKEGPLVDEPIRNVKFKLLGATIAPEAIQRSSGHIVPAARSVTHSSFLVATPRLMEPVFVVEVVSPIECLSAIETILTRRRGHIVQDSPKPGTPLHITKALLPVLDSYGFETDLRSQTQGQAFCLCTFDHWQVVPGDPLDKTIKLRTLEPSPQPHLARELLIKTRRRKGLSEDVNFSKHFDDDLLLSLASQNENQYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PND1</accession>
    <name>A0A8J4PND1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glycerol kinase</fullName>
            <ecNumber evidence="3">2.7.1.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_009102</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FGGY kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.30" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000637">
        <property type="protein sequence ID" value="KAF2069576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PND1"/>
    <dbReference type="OrthoDB" id="1728974at2759"/>
    <dbReference type="UniPathway" id="UPA00618">
        <property type="reaction ID" value="UER00672"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004856">
        <property type="term" value="F:D-xylulokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004370">
        <property type="term" value="F:glycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042732">
        <property type="term" value="P:D-xylose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005997">
        <property type="term" value="P:xylulose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07808">
        <property type="entry name" value="ASKHA_NBD_FGGY_EcXK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000577">
        <property type="entry name" value="Carb_kinase_FGGY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018483">
        <property type="entry name" value="Carb_kinase_FGGY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050406">
        <property type="entry name" value="FGGY_Carb_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006000">
        <property type="entry name" value="Xylulokinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01312">
        <property type="entry name" value="XylB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43095">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43095:SF5">
        <property type="entry name" value="XYLULOSE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000538">
        <property type="entry name" value="GlpK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00933">
        <property type="entry name" value="FGGY_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0859">Xylose metabolism</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY N-terminal" evidence="10">
        <location>
            <begin position="7"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="11">
        <location>
            <begin position="266"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2069576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="513" mass="56716" checksum="77D6EC63ACBEE7A8" modified="2022-05-25" version="1">MEGQQFILGIDLGTSAVKVLVCDRHGNIVTHSTKPYELDQSGGVGYSEQNPEHWVHATFDAIKEIVHSGLVKSDSIAGMSFSGMMHTLVLLDKQHNVLRPAILWNDTRNSKQCNDINKIIGSKKFIEITGNKPLEGFTLPKILWVKENQPDIYSRMATFLLPKDYLRFRITGKIGMELSDASGCVMMDMKDHKWSKEILDPLGIDIGICPPLYQSLEDAGPILKEVCEKTGLSINTKVFFGAADNAAAAIGGGAILSPNQALSMIGSSGVVLNHESEFINYNGEIHTFIHAIPNQYYSMGVTLNAGYSLSWLKNILQDPSIVSGPDDIKTLVDIAKNESCVGAKGLLFTPYLNGERTPYTDSQIRASFIGLDSTHKRGDLVRSCLEGIVFSFQDIFEIYNRVKSGSSSIDHVIALGGGSKSDLWMQIQADIFNKPILSLENSQGGPSLGAAMIAAVALQWYPDFEQCSKAFLKFGKSFNPIAENQEKYSKLYQIYRKVYESTKEICHQLYQFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNK9</accession>
    <name>A0A8J4PNK9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_008298</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tweety family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000499">
        <property type="protein sequence ID" value="KAF2070385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNK9"/>
    <dbReference type="OrthoDB" id="19747at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005229">
        <property type="term" value="F:intracellularly calcium-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072320">
        <property type="term" value="F:volume-sensitive chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006990">
        <property type="entry name" value="Tweety"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12424:SF8">
        <property type="entry name" value="PROTEIN TWEETY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12424">
        <property type="entry name" value="TWEETY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04906">
        <property type="entry name" value="Tweety"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0868">Chloride</keyword>
    <keyword evidence="9" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035216209" description="Transmembrane protein" evidence="15">
        <location>
            <begin position="22"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="270"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="298"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="438"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="519"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="529"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2070385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="546" mass="62001" checksum="37D85003482D2838" modified="2022-05-25" version="1">MIITKSIFLILIFIQIQICFSINVQQPQTQQNDIVRHNEVILDSESDLSTSDTFDQVISSNPQLGQLNSSQLSSNVQSFYSKFPRYDFKGNSMNNTFAPFNRTYIESIILTGLPFDLIIILIIAAMIILVIGYSIYGIVKLIKNKSKFKYYRLLRISKAYSRVRNTPKQLVLIILTVLVLLITCTCLLLSIFVNQDLDKAILLSVDNLSSHIQNRVLVEDRIINETKLLGFYANLPFDALEIIRGTKMLNNVTLEAQQMIHKYENYRYQVIFFTTIALFVVSAIGILSVILRVKWSHVLFVGLGLVCIVNVWTIPASHIPMGVLISDLCPQMDNMVQSYLPSTIDPAYIQFFLNCTDQDSFIFVYQLLDQSVDIAQDKLNIAISHHYSNKTIDYLEKRLSYLTNLSVDSKNMLNCNVTSNTYKSTQNIICINILNSSFTLLILFIGIGLLLSLAFILSLCLYKSSKGMLIVFVPKKDYIDLKSPLLKCSKTINSTYRLSGADDFDYDYYYQSPLISEADVNSDSEEDSINNNDGNGNSNNNNQYII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNN3</accession>
    <name>A0A8J4PNN3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">50S ribosomal protein L13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008258</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000493">
        <property type="protein sequence ID" value="KAF2070430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNN3"/>
    <dbReference type="OrthoDB" id="274622at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_uL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_uL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005823">
        <property type="entry name" value="Ribosomal_uL13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_uL13_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01066">
        <property type="entry name" value="rplM_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL13M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="223" mass="25743" checksum="FB57D86DF95410BE" modified="2022-05-25" version="1">MSTKHIYQNPITSAHLWHVIDATNHHVGSLATKIAGLLRGKHKPIYDRSSPLECGDYVVVLNSRNIQLTGKKWDQKLYRRHTGYPGGLRETPASQMLEKDPNHILRHAVLGMLPKNDSKYYLEQRLKIYENEKNPHIGQISKTPQSSLALNLGPYEAPTINPTNEQVDNYFAGYILKMDDNVEQFSMVESKVRDHKDNLKRQRRVLRKKRANELKPINIEEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPM8</accession>
    <name>A0A8J4PPM8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008976</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000610">
        <property type="protein sequence ID" value="KAF2069709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPM8"/>
    <dbReference type="OrthoDB" id="3219396at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="9"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="258" mass="30353" checksum="D2C8EBEAE36C633C" modified="2022-05-25" version="1">MEKGIKRQLTILQQLNYDSLLYIFSFLTPKELCQLCLVSKDFKVLVEDEWLWKLSFKNIDYLFELYFGIKYPGVVQNDHFQQQCITTSKKLYTYEYCKIEKAKKFVGLWSEKWCDVDVPCGTKIIYDGHTFSIQYTKNKFSSQFFKYDDATDTLEFFLTGGDSGWSFLYSLKPIQDHLGVLHLTVQRIHDNTIFQGIFVQGPPDSRFVNNNSNNISSVGQENLTFDTNLDTNLLQYYQNHIHTSRRNKLTRYVPPLQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ78</accession>
    <name>A0A8J4PQ78_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006666</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000318">
        <property type="protein sequence ID" value="KAF2072003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ78"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36911">
        <property type="entry name" value="LIM ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="168"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="216" mass="24995" checksum="EE16E30D8B1EF299" modified="2022-05-25" version="1">MTTMGSLEVVSINQLIESNMQTTDNNTSISSLYSKSRSNDDFKQQVLKYFGRGQTVDSIERFFQLLQNHKNPRDLIPFIWLFVGESPSLTILSSILDIYRLLFFKDPTILHLLAYSFTHVYITKESFKDVNQFLHEALSTPLTCLEDYDILISIFKLYIKTTSNNQRNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ79</accession>
    <name>A0A8J4PQ79_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_007596</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000414">
        <property type="protein sequence ID" value="KAF2071092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ79"/>
    <dbReference type="OrthoDB" id="21643at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12226">
        <property type="entry name" value="RRM_NOL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034138">
        <property type="entry name" value="NOP8_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48029">
        <property type="entry name" value="NUCLEOLAR PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48029:SF1">
        <property type="entry name" value="NUCLEOLAR PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="84"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="42"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="179"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="267"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="455"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="294"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="374"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="455"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="475"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="515"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="528"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="541"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="589"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2071092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="608" mass="71632" checksum="82EB7C4983B4E535" modified="2022-05-25" version="1" fragment="single">MDDFGFRFGGMMDFFFEEKKKEKEEEEAKKQEEEAQKLVEEELAKEKEIQDAKDAKEKEEREKREERERERQIEAAKYPQSVRLYVGGLNPSVQFNDISNLLSSMIKDTKLEQLDLIKDKVTDQYKGFAYLNVYFPTIESFNKCLNVLNKTKWKGSTLSMQKAKPTYLDKLELMKKKEQDKEDEEKRIKDEEEAKKEEEFKKKQDEEIQKWRKRARIEKKSMYAMYEKYILYKRDHPEFAKDKRHSTLPTVNKYANLGKEYSFLESLLDDENQQQQQHNNAVSFDSDQEDNGMDYDNNNNYNNGSTSQTVPKSQYYEDEWSIKPTNKVQQQQQQPNHYQQQQQPQNNNNKKRNYQEDEYYQDDYYGNDGGYNGDDGYQQQQQQQYNDNDYYGNDDYNGENEDGYHYNEDGEKVIDYEIKKQSASEGERKRLEAFKKRRMQQQALTQSVSLSLSLASNDNQVITFDSSDDEKDTKSTTTAPKKSISIPWKKIPRYDPKLLNCTDLELNSDDDDDQEKDKQESDKDSESESSSSSDSESSGESSDDESSDDDSNDQEDKENEGDKDNESDKEEEGEEKEEKESSSSSSNESEQEESDKEEETEEKENKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ86</accession>
    <name>A0A8J4PQ86_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006652</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000317">
        <property type="protein sequence ID" value="KAF2072018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ86"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2072018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="82" mass="8683" checksum="B9BC8E974946C853" modified="2022-05-25" version="1" fragment="single">MSSESGPLHPSTLEVPLSLLTAAYHKDNDIHKADPENAKKIGVPRALISKAASPSSNFDPAGSQTCTSNFQIDKAIKLKTPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ92</accession>
    <name>A0A8J4PQ92_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphatidylinositol glycan</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006960</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000345">
        <property type="protein sequence ID" value="KAF2071724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ92"/>
    <dbReference type="OrthoDB" id="331263at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007245">
        <property type="entry name" value="PIG-T"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12959:SF11">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12959">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-T-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04113">
        <property type="entry name" value="Gpi16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035146317" description="Phosphatidylinositol glycan" evidence="2">
        <location>
            <begin position="40"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="571"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="627" mass="71572" checksum="7B1AEDC2603D2AEC" modified="2022-05-25" version="1">MMITTTRETRKGKSKLAISIATLITCLLLLSSFTQTTSATISTEDDDYITFTETQSFNEEMIIKPLPKGKSMMHFQFSTEWNSNFFDQSTFQHYDLFPRSIGDLINRVGIEEMTLVFTKGRWSYSEWGYPIRASPVGVELTAWLRPLEKGIDSQWRELTHSLSGLFCASMQFLYQVPHHTSSPSRSFRPEGTSNIYHTMERDTVPAIELRNQSSVIPLELRYGILPRESVCTENLTPWVKLLPCREQSGIGQLLNPLKLYDVHYHSMSITVRKICKSSNNDCLIPTLEIIQSLTVVNDVSNSIDNIDKNKVKVALENLFEKSTGLYSCPLATSSKIYVQNQENLNTQLTVTPTPTTKNTRFSIYDLKQYSIQNPLFLTLSWLKNFARSGPTPIVAHSHLTGYGQERGGIATQIVNQDQHPMNITYYHAIPWYLRLYFHTLRFSIQDKEYSDKDFIFKYISPAETRSSPSTIELSFEIPGNSVASISIDFDKVFLHYTEHPPDANRGFDLGSGVITAYIDPNNIVPGSSLSQDTFDIEWSENTYPHQKHQVTPVRIYTEGLLITLPTPDFSMLYNVITLTGTVFALFFGSMINILIRRFKNTFTGDDFVSDRPIAKIYRRIMKFIDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQD5</accession>
    <name>A0A8J4PQD5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Polyketide synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_006959</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pantetheine 4'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:47942"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000345">
        <property type="protein sequence ID" value="KAF2071723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05195">
        <property type="entry name" value="enoyl_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00833">
        <property type="entry name" value="PKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.366.10:FF:000002">
        <property type="entry name" value="Probable polyketide synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.110">
        <property type="entry name" value="Polyketide synthase dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013217">
        <property type="entry name" value="Methyltransf_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032821">
        <property type="entry name" value="PKS_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042104">
        <property type="entry name" value="PKS_dehydratase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049552">
        <property type="entry name" value="PKS_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049900">
        <property type="entry name" value="PKS_mFAS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050444">
        <property type="entry name" value="Polyketide_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45681:SF6">
        <property type="entry name" value="POLYKETIDE SYNTHASE 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45681">
        <property type="entry name" value="POLYKETIDE SYNTHASE 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13602">
        <property type="entry name" value="ADH_zinc_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16197">
        <property type="entry name" value="KAsynt_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08242">
        <property type="entry name" value="Methyltransf_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21089">
        <property type="entry name" value="PKS_DH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="Probable ACP-binding domain of malonyl-CoA ACP transacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52019">
        <property type="entry name" value="PKS_MFAS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="7">
        <location>
            <begin position="37"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="PKS/mFAS DH" evidence="8">
        <location>
            <begin position="946"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal hotdog fold" evidence="5">
        <location>
            <begin position="946"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal hotdog fold" evidence="5">
        <location>
            <begin position="1094"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for dehydratase activity" evidence="5">
        <location>
            <position position="978"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for dehydratase activity" evidence="5">
        <location>
            <position position="1152"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS52019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2071723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2221" mass="247018" checksum="89D0B33F5FEDBF2E" modified="2022-05-25" version="1" fragment="single">MQSVNNNNNINGSDNSTPNTSPDISYQETHTTTQYSANDIAIIGMGFRLPGGSKNPNELWNGLMEGFDGISSVPKDRWSKNYTEVGLTKTKLGGFLNDSEWKTFDPLFFGISPKEAPFIDPQQRILLTCAWEALEDAFIPPSELRGTNTGVFIGVSNQDFLKIQYRDQNEISPYSSTGTNSSIVANRVSYCFDFRGPSLAIDTACSSSLVSVYLATQAINQGDCDVALCGGVNYLVDPTTSIGFSSLGVLSEDGRCKPFSDKANGYVRSEGAGVLVIKSLDKAIQDGDRVYGVIKGGAINEDGTFNKSSLTTPSSDSQSNNITKALERSNLSCQDIYYVEAHGTGTAVGDPIEVGGISGALSNDENTLKIGSFKSNIGHLESAAGVASLIKVCLMLKNRTLVPSINCSSLNPNIPFADYNVEVVTQSEPFPKDRLVNIGINSFGYGGANCHLIVQEFNNDLNVLLNDNVENDSFKYLIPFSTNSKQSMDKYINLIKSNPSYHTTMLFEEFVKHQIISKSHNLSHRAVSLSSSWSNFINNSTMINSTKQLSKDTTGKSSIVFVFCGQGPQWKGMGLELYKSDKVFKDTVDKVDGLLSNYYGYSIFEKLSQTPDNSNDIHSPLLAQPALFLLQVALVEVYKHWGIVPSVVVGHSFGEVTSAYTSGIISLETAAKIVYYRSLNQAKTIGSGRMLVINVSSAKWSEKYSTLFPTVEIACYNSPDSIVVTDDNETLDSVAKQLTADGIFNTSLKSPCSFHSRHQKVIRQSVMESLGEIESNPTTSIPLFSTVDGNIHTKQYSANYVYKNLRKPVYFEKAIHNICKYLKEVSDNPIVFLEISPHPTLNPLISKCIPKGLKSEPLVLGSLNRTKDELDTFKSSIASMYTFGVEVDFTYQLYNAVINEKSIAQDFTSNYWKDVTSVLPKYQWDSETYWNESFESKKTRLDGPSTTLLGNQLSGYSVPTFETIINVAKPIFAYLADHKVKGKSVFPGAGYIEIISQYFIQLQKSNNSTTGKDIVIESLEFMNPFVIVDGENQTLQSIFEPIAKDKYRISFLLKDSNQSKDELPTWTKTCKGVVSLYPHNANINQLFDNNSLATNVVEKSNLYNHVSLKLGLQYGPNFQRVESLKVSNDQSKSIATLATNNDKSILNASILDNAFHGMLGMVNDKQDYVVESIEHLVIYVNNLPLVCPKDIKLLTTVESKKIFSIQGKAQMISNEKVIATFGKITIKSISQKKKSNDNSDLKVPSKETFSLDWQSKDSPIPSPANLILAPFVNEESFVASNILQDSLFCQYCSNILYENLVQVNQDFSLALKDSKSFIEKYQVKVDSLHLERFFSRILEIFTLYPPQILQVSKQAVLDKYPKSMLELDLIERVAQVIPKLVLLGDSSAPHTLFEDDILSRFYSSSISTTFYLEKVSLLTKQAISSVVNEKRVFKILEIGAGTGSLSWKVLEQIHNTLTTSSNPESLIVEYNFTDVSLGFIPKDLQANMTSRFPLIKFKFSTLDLEKNVLEQGYMANHYDLVLMAYVIHAVSDLDKAISNIYKVLSSRGWLLFIEPKSNVVFSDAVFGCFSQWYQFNDSIRTNHCSITPGKWNEILANHGFNNTKCIAGDTFDQSHTFIIHSQKNSINEFPLPSTGFEKVLIVMDKTQSQRLTTHNTSQKLLKWMRDISALFPKVEMIDTTAFTDGVHLAQQKVQDCTHILHLVGIDTLNSNYKSCNVELVKLVETLSLLKSKAKLIIVTKQAQKSPRNYINSSFIGVFRTAQNEHPNLDMYSIDIDCEENTSIHTIFGITHMACSDKEFIMKKNISFVPRYFNYNLPLKESTFANADQVFCNANASLNFKYHFKPNPSSNSVTIRVMAVGINFKDHLFFKGQLPQEIFRKGDVHHPPFGLECSGIIEYIGDNVTEFKVGDHVMGFARHSLGSHVQTSKNLIIKKPDNISFIEAASIPVVYCTAYYSLFKVGHLDSANESVLIHSATGGVGLASLNLLKSNGCKEIYCTVGSNDKQKYLESNYTLSGIYNSRDHLDFSSKLDHKVDCVLNTLAGDAINSNFHCLRSFGRLLDLSVTHIYANEPIGLGNFKRDILYSTIDLERMIDEKPLLLKNMLNDIVANISNGTLDKVPIKSFQVDQSKNAIDYLSERKHIGKIVIDCSDIFNVCPKPTNIGIPKSQYKLDVGPCALITGQSGLSIPLIKWILEHSSETTDIIVASISSIKPALKALMDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQH0</accession>
    <name>A0A8J4PQH0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ras guanine nucleotide exchange factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_008386</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000512">
        <property type="protein sequence ID" value="KAF2070294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQH0"/>
    <dbReference type="OrthoDB" id="546434at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF319">
        <property type="entry name" value="RAS GUANINE NUCLEOTIDE EXCHANGE FACTOR Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="5">
        <location>
            <begin position="782"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="4">
        <location>
            <begin position="953"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="77"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="166"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="280"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="564"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="166"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="583"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="611"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="629"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="673"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="718"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="732"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="744"/>
            <end position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2070294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1183" mass="132782" checksum="C9396BA86548620C" modified="2022-05-25" version="1">MDNSNTLQDTKGVSTSPPNTLPIHSYVQKLTQQINNNNPTTDSIPNDLPSTIPSVKELGKVYQSHADNHLKQQKILLSSSSSSSSTANTTTTSTSLTKSNSISPTLSKKITTYNNNILNNNNNNSSSSNNNNNIGKINNNHDYIEKVVKGNFAEQSSQKNKLNKNIIKDNSNSSTNNNNNSNNNITQNNNQDSGNSGVQDNLNNLNNTLENININTDNSDNNNIDISTDNNSIPKSLSTESISSFINSRNYQSHRVAGDWKDKKMFTQVSVSNPKARKALIGARDISNSKSDEFNDNNNNNNSNNNNQNINSNENQEEEESIKFLSKQFVNRKLNNRTSDRKSKLTKQDAEWIVKNFVDESEKDEKNTILNNKKLANRSKFKSYHQSIRTKNNKIDDGDNGKDLLSTSGNSAFTNPNSTIKEIDQENIVEQQLFKELDPLANPSWRLLEEVGKKKFISSEPSRRPGIKTPLFSNISTLANPPQFHQIPPERISSNLFTTPVQENSYPDTSLLFNQQIINRVANDKELSYLFFPVLKSSKEISFKNPSSSSSSSSSSQAMVANVGANHKGGKPLSPNLSTGSNPSSCDSFSLSMSPSPCSSLSPSPSPSPPLLLDSMSPLSSPSSFNNIIKDKEKEKEKENSKEGIKEKDKEKDNKEISKESTSRPRSYWALKSSTKTQIQSQSTGTLTTTNSITISTSPSQSVSFSPTDSTLLDQSINNNNNNNNNNNNNNRPSSPTNTNLENSISTSPPTTSTVETISTIIQEEFSNRTDIILCPNSIQKNRIKVKSATIDALIQLLTHHRISQPDFIETFLLTYKSFVSPLVVLDKLIEKYEYDPFDEDLNQQPSSPEEIEMKIKNSRTVKLRVVSIIKNWVDKHFYDFEKNPHILEAIVAFLEGPVLEDGMEKVSSNILKLIERRKTGIDKEKNTLHCKFPTPILPILKGDQILTFYHFDDLEVARQLTLIEHESYSLIKPNECVNQSFSKSGKEENAVNIVNIIKRSNSLPLWVATEIVQEEKIGKRTNIIKKFISIADHCRNLNNYNAVMEILSGLNLTPVFRLKKTWDQLPRKYLATFKHLNALMAPKFNFKVYRDVLHTKNPPCLPFLGVYLTDLTFIEEGTPDTLENGHINMVKRSQVSSVIKEIQQFQQLAYSFTVVPQIRDFLLQINGLQERALYKQSKIIEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQJ2</accession>
    <name>A0A8J4PQJ2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ankyrin repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_008341</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as a molecular chaperone for G protein-coupled receptors, regulating their biogenesis and exit from the ER.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000505">
        <property type="protein sequence ID" value="KAF2070339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQJ2"/>
    <dbReference type="OrthoDB" id="1585644at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021832">
        <property type="entry name" value="ANKRD13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055285">
        <property type="entry name" value="ANKRD13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12447">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12447:SF25">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 13C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11904">
        <property type="entry name" value="ANKRD13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 8" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="559"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="601"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="645"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="61"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Ankyrin repeat" evidence="11">
        <location>
            <begin position="177"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="295"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="519"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="721"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="796"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="295"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="313"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="340"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="726"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="804"/>
            <end position="831"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF11904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2070339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="840" mass="96557" checksum="2FC27701EB8874D8" modified="2022-05-25" version="1">MSTDKTTTTTTTTTTTTNSSNHEPFNPFLLHKCVVKNDKEGLKVLLQDPMYRTYIDIRDRCGYPASHYAAHLGYKEILELLLKNGANPNKKSAAGWSIIQEACGRKDREIVGLLLMNIKQKLEGEFKSRLPSLVSGLSNLPDFEMEMKWEIKSWVPLVSRFCPYDTYKIYKRGSSFRVDTTIVGVNGIKFQRGDLVFMFTSNRFFSVDNLKKTYVEFSLVSKNTSTQDEEISIMLHNNIKRIKILTDKIEFTPSKSWFGYEKTEKIGDNNEWNAKVYEVQGIDLKILSRKNKKDMIVRPNKSDDQDNKQENGQDADDETEGADEELSIDEDDQLVEDNDSNNNNNNNNSTKNTSNNNIKDQLKKRIEEAKAANYQIRESSIKSMLEDPDYFKLPKNFDLENHEANIDKAKLKTKGLLCEGESVSEKHKLFKGTMWISDEFPRKVSDLLPIFEALSPTNKLFSRLSEFISLKLPSQGFPVKLDFPVFPTITATVIFTKYTEKEIKSELFNIPKDYVRKQSDKMKDANTKTSTQQEHQDKDEATTTTDTTVDDGDSSKLNLVWFIFKILSVCFSYRVVFLFAPNILNDDKDGEKKSFKQNVDSNFYSLFSDSICVFMFFTTCILFFWRAKSLIKSLKSSEYSEWKSLIWAEFYGGGIEFGAFMLLLLLKWPVIFSYYWYKGTEQEDKSTWAQMFMREFSPKGPNREKDEKLNPDIFFPPSHPDYPRQSASSASGASSTGKGKVTDEEWRRTFRRDMQNIGFEFDQNGEITKFPPTEVMEQYQKSERFRDLMFMADSIGQPIIEKQSSSSNSSSSMPSIVKPTSTTTTTTTTTASKKKGKKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQM3</accession>
    <name>A0A8J4PQM3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000287">
        <property type="protein sequence ID" value="KAF2072355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQM3"/>
    <dbReference type="OrthoDB" id="16075at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019396">
        <property type="entry name" value="TM_Fragile-X-F-assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13568">
        <property type="entry name" value="FAM11A, B PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13568:SF9">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="295" mass="34744" checksum="63E9DEB83A26DE51" modified="2022-05-25" version="1">MFRDIINNDRAYTEHTDDNPSTIESFRLKLGQVIGARFIASFIGIFISTIFLNLRLANFIDWGYYFIFLPVAVIFIPCFFTPLIQFFTKKVMEIKRNQDPPTTKSKRFFYYFLIWAPFIWFSIFVAAIILRLEMGKRFQSFHIFVPIILISLALSVICYIDRIRIRASKLIYIVCCFLLILFTTFVWIRFDKRTPSFNWVYIFVPLYVGEVLLLIDSGIQAANSSGCIFKRAFNHFFGKVLVLVPLTIFFIFLSIYLEYQSIPITMVFIPLYVAEVFVFFLFVYMAIIFLVDSVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR42</accession>
    <name>A0A8J4PR42_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein disulfide-isomerase</fullName>
            <ecNumber evidence="3">5.3.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_007219</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Catalyzes the rearrangement of -S-S- bonds in proteins.</text>
            <dbReference type="EC" id="5.3.4.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.4.1" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000372">
        <property type="protein sequence ID" value="KAF2071466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR42"/>
    <dbReference type="OrthoDB" id="427280at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057305">
        <property type="entry name" value="Thioredox_PDIA6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45815">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45815:SF3">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24541">
        <property type="entry name" value="Thioredox_PDIA6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035303230" description="protein disulfide-isomerase" evidence="7">
        <location>
            <begin position="21"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="19"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2071466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="413" mass="46553" checksum="C7EDC1EC4E5DC8CB" modified="2022-05-25" version="1">MKNILSIFLLFTLFVSYSFGFYENTNVVTLTTKNFASQVFGSEHIWMVEFYAPWCGHCKSLKPEYEKAAKNLKGLVRVGAVNCDEQANKELCSNYQIQGFPTIKYFPSKLVPAKAKGTFHKVPEDYQKGRTAKDIIDFALSKLPSFVTKVTDEKSVEKFITSTTTAKALLFTDKPKTSNLYKALSVDFHNDIALAEGKSLAESILEKFQVTSFPTLLVFPSDDQSQFTKYEGKLEHESLFKFLSKYQTKGNDKQQQQEPAEQQKAPAEPTLDYALEIKDKDTFDKHCGKGVCLVTLFDLQSEDDKESNQQKIQLLNDLAKKNAGRIKIVWADGSIQSNFVKQLDLAGLPSVFVLNGNKSKYIKYLGSFTEKNVQEFISNLNKKTAVDLPKGLPEFINSATTTESKKSNKKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSH8</accession>
    <name>A0A8J4PSH8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004972</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000187">
        <property type="protein sequence ID" value="KAF2073713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSH8"/>
    <dbReference type="OrthoDB" id="18554at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00821">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="5"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="302" mass="35817" checksum="10911FAF88630112" modified="2022-05-25" version="1">MTEMEETYEAVLFSQLTNDEGHQHPWEKKYLVMDHHSLTFRESKEDTIRRGVIYLSDVVYCEVSEVPNPDHENILEIKTRSRSTYLFSFESESQMRTWETVFNRHKGVMTQRDLEPPPPPPSQRAHKKERYTISKFIENRRIFGGMWMVYLFIVYTIVLVVLFSVLWTQYVLPLRDITQESSTCYIHSAQAEYRWKKNYFLNIRVTYTTQEGEVLDNWMQQACAGKQCPSFMEENYIPGTEVKCYYDADDKNFVFFNLKPFVNWRYSICFGISGGLFAPWIICVLVIGTMSRKKIKEMLMCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSS3</accession>
    <name>A0A8J4PSS3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Deacetylase sirtuin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_005910</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sirtuin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000248">
        <property type="protein sequence ID" value="KAF2072787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSS3"/>
    <dbReference type="OrthoDB" id="420264at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0085020">
        <property type="term" value="P:protein K6-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF8">
        <property type="entry name" value="BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13857">
        <property type="entry name" value="Ank_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="137"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="213"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="246"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="317"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="357"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="9">
        <location>
            <begin position="441"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="46"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="570"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="579"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="582"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="603"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="608"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2072787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="723" mass="80022" checksum="FF9283B94B7AEEC1" modified="2022-05-25" version="1">MDNNTTSNNNLLGEKIAIALGTLALGVLSCKLFNKYNKSNNTTNSGVNKNSNNTVNSDGTQEEFKPENLKWTPLMIASYYDDYEEAVKYINQDSNSIHQVDVNNWTPLHIAVSRNSEKVIDLLLQNPSIDVNIPNNKGYTVLHIACSNGNLNIVRKLLELDVHLTICSNNMESCLYLACSNAHRDIAVLLVEFSAKNKGDEQTREIINQFNIHGVAPIHLVVLKRDVELLGLLLDHGADINAFKKDGSTALHVASIVDCSEAVDVLIERGADYLVVNRYGNSPLHEASIKGNCSAVSAILAKAGGQRENLANLKDKDESTPLHLACNIVNAQDTGRYLKVIKVLIDAGANVNELDAGKATPLHIIACVGEKGTEIMKYLLECGADPTIENQSGWTPLHHAAKQPAKVFDVLSAWCKKHHPLFLSTFDKDKPRVIPVRKPLATPSESGLERLTSIAQGIRENKYKKVIFLTGAGISANAGIPPYRTQDGLYDASKQFSFSAQTIYDTPDVFYDGMRKYFYPVVTGHIKPTPSHHFIKQFQDRGILLRNFTQNVDQLQEKAGVDDKHIVHAHGSLGDDWYCMGCQKKYTPSYVWTEVGRNVEPYCDVQTCRDIIRPSVIFFGEGLSNQFHQRSISDLRECDLLIVMGSSLVVYPFAGLLNDVLPNTPRILINKEPTGPFKSVSDIDKSPTNDDLVIESRGSRDLVILGDCDKGVDYFNSLINQSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT03</accession>
    <name>A0A8J4PT03_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BUD13 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009787</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CWC26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000806">
        <property type="protein sequence ID" value="KAF2068891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT03"/>
    <dbReference type="OrthoDB" id="6022at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070274">
        <property type="term" value="C:RES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018609">
        <property type="entry name" value="Bud13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051112">
        <property type="entry name" value="CWC26_splicing_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31809">
        <property type="entry name" value="BUD13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31809:SF0">
        <property type="entry name" value="BUD13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09736">
        <property type="entry name" value="Bud13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="63"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="112"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="192"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="227"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="295"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="327"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="396"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="432"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="471"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="485"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="502" mass="57273" checksum="C1718E25041A7BFB" modified="2022-05-25" version="1">MNDYLKKYLSEPSNNSSGNVKKKKKTVSKPISSSKIVDIDDEISSTSGYNDDDDYNENDSFSIGIAKKSIATGKKKSTTTTTNNNTISTPPTGSWVTIEPKNNNTDLSPPRKSTDRDRDLSPPRRRANRESDDLSPPRKSINRDRDDLSPPRKNVNRDRDLSPPRKSVNRDSNDLSPPRKNTQQDLSPPRKRNNDDLSPPRKRNNDDIDLSPPRKNNHNNNNNNTPIDRDLSPPRKRDSDDLSPPRKRNNDDIDLSPPRKRDSDDLSPPRKSNVPANQRLKMSFGGDAGLHSGKTIKEQAMKRLQEQKDAFERADQKLLGKGAQTVYRDRASGKKIDVEQERIEKQQQQQLEREKKEKEDQEEAEMDWGVGRVQKQHKLGKEQQLLEEMSRPLSRYQDDKDLNDSLKDKEIFGDPMAMFKSTKKKKKEKSSSKSSKSSSKSSSSQSKPLYNGPAPANRYGIRPGHMWDGVNRSNGFENDYFSQKSKKDNMSEEAYKWSVEDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTI3</accession>
    <name>A0A8J4PTI3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_004307</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000150">
        <property type="protein sequence ID" value="KAF2074373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6019893at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032483">
        <property type="term" value="P:regulation of Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005112">
        <property type="entry name" value="dDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051696">
        <property type="entry name" value="DENN_Domain_GEFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022096">
        <property type="entry name" value="SBF1/SBF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005113">
        <property type="entry name" value="uDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296:SF21">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03455">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12335">
        <property type="entry name" value="SBF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03456">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00801">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00800">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="C2" evidence="5">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="UDENN" evidence="6">
        <location>
            <begin position="426"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1487"/>
            <end position="1529"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1530"/>
            <end position="1570"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1571"/>
            <end position="1611"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1612"/>
            <end position="1652"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1653"/>
            <end position="1693"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1694"/>
            <end position="1743"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1744"/>
            <end position="1784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="101"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="191"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="242"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="290"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="527"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1062"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="113"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="195"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="305"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="322"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="527"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="539"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="553"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="569"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="595"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="612"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1062"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1082"/>
            <end position="1098"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2074373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1822" mass="205523" checksum="DB5AF99C2C6C7742" modified="2022-05-25" version="1">MSTTPPCGCTRITTLFIEDFQAKNITEKDTFVKFNIDGKKYKTKVHKKTLHPSWSDKFTVTTTNKIEELTFSIISQNILGNNVKIGTAKYSLSLNQQQQQQQITNPLRSSTGSLSQPQSNVQQQTNSNNNNVADINISSSPSVQTPNHHHHENGNSNTTTPNNNDSTITTNSSVSDSLETFSFDYWTPIEREKGSSGSASSTPSPSYSSSNPNGVSPSSNLSEFKVKILVKCRDIYLDTDTNSATSLHDEPAVQAVPPSKDFKAESANPKEMSIIGKGGSQILKLTKKITSKSSRTIGGGGVNGVNGSPATPSPHHGGSPHYPEKTKKEQQAQQRIIEEQLAKERQKEIERENAEREKERAREKLRQREQEEQQKQVEKERQEERRRAKEDKKKKEKELKYVPDRPIDYFFVVGLSTKLEPIDQRYDFSPKGTSPIDKIYKGELLEYYPHKENNDILPKHIWMYCYPKGISLQSDPQQPSFFPFVLTSETGSRFYASCLTFYEPISDDLYSELKKNTIAYEHSLSTPTVTLNPENESSTTTTTTTTTTTAATTDDKNKESTVSKDVSKDSTPSTSSSTTTTTTTPVITTTNVLNNDKDSKDTTPAKDTSKDTTSTNLDTSISTSSTSSTLKRIQSGNTIESNFPSVLYAPKCICLLSHFPFFSAFRISLNEIYHKVFFSSTPLPIERYIYNLVQEIPLPQPGANIVSFNLGGQTQIPLKRPSDCLLPTPDLSFNILFKCLDIRNVLLVLKCILLEEKIIIISSQYSLLTYISEIISSLLYPLQFPHVYVPILPELLLEYVYSPFPFIMGVHKSYSSTILNEENLLSEIVVVDLDNNTVHIPPTDTKDIQLPERETAQLINQLRRVVQFEILSADLPNFNLNTLLPISSSYNTDKRLQNNNNQKQQQPLPLPLAPTHPDDFIRFSFLQFFCNMLGDYRKFYKYLRVFPQPIAIFNKEEYVKSKPQSARPFLTRFMDTQSFSFFLEQHNWPTKNLFDYLVETKKYTRPIDDLVAQYQQSIPQALGVTDANKLPVINVPSPASYRSYSTSIREYTKFPSLKQDLFNPNANNSTGSNSSVNPIIKSNSASSTPSQTSPSVTPLHDMYIHSNADHTPSLAEQAAGNDILATFSEDQHRSFVPLIRQFLTKVFNDTLPEQSDIQQMTELVKFDYGRSIFGKLLLHHYYRQNYVSSCTSMHNLLGGQQDNNHIDTTKARLSDPLFYCLGDILKNALREANFQSDFTTSQMFLEASFIYHRLQKGSNEFVSERIRNLDIWQNYRFWEKIFYDSIESKCKSLYGNNVVREMVKWGQYPSEKQEKMMVEERDMSFSLLSNLVFNMINLGSQTDLVRRFVNKMCTCINFDAERTDTMMQIVSNISRARDMVDMDQDAIVEGAVENNDKHILKDKNNKEDLYLPIGSGGSGSGRDQLNHEGRELLNSLIDEKSSVVAIKSFSRIMNLKTTWHENRSKKHDKVAFREISENRGDYVVKTFTGHQEGVLCVSVAQKESGLLITGSADSTLKVWDMTSTRCIGTLEDHSGWVTACETTSDSKLISGSYDKTLKLWDLNKCIKVKSLRGHKGAISCISNADTNIVISGSYDNTINVWDTRNAKPSITLFGHQQPIMCLVASDAYKVISGSRDTNIRIWDIRTSTSISILSGHSDWIKCLEWDNADLLLSGSSDGRVKVWSLQTGECVKTLQSHSGSINSILLHSKVENDGSSAPKKFITASSDSTMKVWDSNYGESYNSLEGHTDEVMNVSKFINNLVVSGSFDGTVKLWDVDAGKCQRTLHNHSHRISSLRTFDSSIVTTSWDKTAKVCQFNLDFRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTM5</accession>
    <name>A0A8J4PTM5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Short-chain dehydrogenase/reductase (SDR) family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_005837</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000243">
        <property type="protein sequence ID" value="KAF2072860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTM5"/>
    <dbReference type="OrthoDB" id="13950at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05374">
        <property type="entry name" value="17beta-HSD-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051911">
        <property type="entry name" value="SDR_oxidoreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43976">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43976:SF16">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2072860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="281" mass="31129" checksum="A5CD315116EA6A55" modified="2022-05-25" version="1">MATTPVWLITGSSQGLGLAMVKKLLGQGFHVAAATRNKDLLINNLKKDEIKFNESQILTVNVDITDNDQVKQCAKKIKDKFGRIDVVVNNVGHGCVGAVEEFSDKEIKEIFNVNVFGMLNVLRHTIPYLRENKDGGRIYNLSSIVGYYACLPLGAIHSSYKFAIDGISEGLASELKEFNIKVTSVKPGNHRTQVLDHFLLPKLLLPEYKTEGVYNTLKSQSGKQVGSPDKLAQVLIDLSKSDDPPIHLFLGQDSLNLQAKKSKLIQTEIDKFLDLSKSTKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU60</accession>
    <name>A0A8J4PU60_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005064</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000192">
        <property type="protein sequence ID" value="KAF2073645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU60"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="291" mass="32682" checksum="3AEDFE3443BF5584" modified="2022-05-25" version="1">MQSSKDYHNIIISELDASRFILLNSIINYFFKSTLANLKVEYPINGHKTTDNNNQNVFQKLNNNGMDHLILKKEKSNYHFYVCPGLTGPDNHRKVVELIKSLPTLSSIVFILNGGLSKLDVSIKNSINQLKGSFPFTIKKKVYLFFSNTTHNNQPLGMAELKSLAIKGKKIFTLNSLCFQSNPDTWKGNSLKKIEMGFSLENSYSIIERFLLSITNSIQSSTSDMGTSLAFVNETKSCLQNARIEVKAVQKTQDATEYHFTGLLKQLKSKVLIITVQCAQDVITPVTKDVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUJ6</accession>
    <name>A0A8J4PUJ6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">S60 ribosomal protein L38</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_008841</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL38 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000587">
        <property type="protein sequence ID" value="KAF2069836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUJ6"/>
    <dbReference type="OrthoDB" id="10250488at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022618">
        <property type="term" value="P:protein-RNA complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.720.90:FF:000001">
        <property type="entry name" value="60S ribosomal protein L38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002675">
        <property type="entry name" value="Ribosomal_eL38"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038464">
        <property type="entry name" value="Ribosomal_eL38_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10965">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10965:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01781">
        <property type="entry name" value="Ribosomal_L38e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2069836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="74" mass="8556" checksum="5871F704DBCCAE88" modified="2022-05-25" version="1">MPKQIREIKDFLVTVRSKDAKEIRIKKVGKNLTKFKVRCGKYLYTLRVTDQVKAAKLMQTLPPNIPTVQIGKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUV5</accession>
    <name>A0A8J4PUV5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003362</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000104">
        <property type="protein sequence ID" value="KAF2075333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="20845at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032045">
        <property type="term" value="C:guanyl-nucleotide exchange factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.980.20:FF:000022">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.980.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31334:SF1">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE PROTEIN SMCR8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31334">
        <property type="entry name" value="SMITH-MAGENIS SYNDROME REGION GENE 8 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="22"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="123"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="235"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="273"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="626"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="789"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="853"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="975"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1055"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1142"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1201"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1268"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1345"/>
            <end position="1414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="22"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="131"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="207"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="275"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="626"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="663"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="673"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="704"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="789"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="808"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="975"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1055"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1076"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1101"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1142"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1170"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1201"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1269"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1302"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1345"/>
            <end position="1383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1404"/>
            <end position="1414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1414" mass="160019" checksum="C2A1690DD2CBE719" modified="2022-05-25" version="1">MNDAKSFHSIPDENTLLISLKSNNSTTSNPIVNNSNTNNNSNSNNSSQQQQSEFNVQPSGINSLLSLSSLSTEFQKRDYTKKEIYDEAINNSFSSLSSNFNTLAPHNHHHIHHNSNNNNNNIYPFEFNVINNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNKNWNTNNNNNNNNINLHNNSKMTLGGGSPSESPPLFLNQSATNTPSISSLQAQQNRIDNLIYSPSSSSSLLSSSSSSTSSSANTSNGTNNKIVGNIFPEISSCTNNANHNHNHNQNNNSSQSPLQLPIQSNNNPNYKPHFNLLYDESYINNASLICCKCNNKTTIDIQVFLENKINVIPGPEDKNYEYRCNDCSKGTFYFRHLFKTWKEMIHTSLFNLEREKRYRFSHYREIIKYVHKNSKELLADREKVLHNKMKNIMTATLSYNRKIFISFARDSGMWSNYRPGDMILEDDRGLLTWKGNDLWEDYIDRDSLHTLICKSCTRPSTFYFRDLIGSTVLPGPSDTRFEFYCSVCTSTRKPTIKHLSKSWKEVAHTALYNLEILKKYRFSHQIEIASYVKSHQDSFAFNREMDNIKNRLSTVLSRYKKAFTSIGRYTGMWSNWRDSDATLQELQDMEDDEFDEDDDEIDQTNEFEGLDIDEIEKRRESQSIGNSPMKSKSPIKSPKKASKAKSKSPTKSSPKVDRSTPTKAIQMSPPSSATSTPNTKIKLAPPIGSPSSDGIFKKIPSLPTISSPPITSNNGSSNINNNNINNNNININQSNHYNSGNNINLNNSGAYLNQSDHNNNNNSNPNHGVPQSGFSPFINQNISPKLSPDSSTTTPILSNSPNFNFYPHQSNVMYGSININNNNNNNNNTTSSISNNNNNTSNNFNNNTNNNNTNNNTYKKYTDSNSNMLPSECYLCRKYKTTGFFKCRNFIQPYQTPCSKVFCKECISTHFPSFVAKYGNEPLYCPDCVFSTSSSSSSSNSLIDHNNSNNINNNNHNNNSFAHPGLQQSLNSSGNNMMIFNNVHFQPSPIDVQNYFGPISDDSSYTSNLSIIKNNIVRNQQQQQQQLQNHHHQPQQQQHNHQHHNTHHQQPHQHQHQQQNHHQHLQIQMQQQQQHLQMSHLTPQNLVVQQNNNNNNIQQPQQLAQSNVNNFGNNNNNNMQNNNNQNYKGKRKAGKDEPILHPLDKPTSPKDLKRAKIDQCEQFNLQNSPPHLTGNNTLHLSHGHIPQTHHQHHHQQQSDMLFQQNIHNNNNHHHHHQPQPQSNYYYEQRNLFNSNNNTNSSSNNNNNNNNNNNNNNNNNINNNNNPQYNNPYFNQLSHSTGSIGQSNFPLPTLSLPNITLPTLNFGSASGDYSSDSDNSSGGGSSLHSNISHSLGANSSSSSSKSLSPEIKIGNGPTSGGKLMGFIQDQEKTKRQIYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PV21</accession>
    <name>A0A8J4PV21_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_003234</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000099">
        <property type="protein sequence ID" value="KAF2075453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="20118at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12087">
        <property type="entry name" value="TM_EGFR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057709">
        <property type="entry name" value="DUF7949"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25820">
        <property type="entry name" value="DUF7949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035261150" description="EGF-like domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="1620"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1581"/>
            <end position="1604"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3 4">
        <location>
            <begin position="1298"/>
            <end position="1309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2075453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1620" mass="180156" checksum="B00062D023F8216F" modified="2022-05-25" version="1">MKQVFFVVFFALGLLLSIVDASSHTILSPLIQGYAQNIADTNTWGCSVDGVVLLTDAGYTPTVASTYISTVIEKNLTDTLMSIKSHGLVMGTPIIIPILWTSGATSITTNIQVDCEQPPVPVITTFSDKMYWIYSNGLINAYPMFVFKIDNFNKKPPPIAAKFPLLNQGADIRMLSNNIFFIHLVSVPELLSQTQIDVMISFDNFITSKTISIKSVINSNTVLNDYTYSGNIFPRNVNNVTNSRFCSVFNKFELKNGPIPFYSLLRVSDSGINQVVKVLGNDVDFTLFQAISPLQPYKSLIVFQPTTNLEFNQTVTFIEPPSATPTGSPALVSTPPTPYRSFHVQFSYTNNIYDIGAVIQIGSTFTLSFLQIYPYGYTSILSSNKYNKIFEFNLNPYFNGSLEYSPLVANPILGDGITPDKDAPIIQFISIEKITGNTPLYVLQAKITDATGFVSLSNFGDYTNLVLGSIKDGVYEFLYDPYLTPRVVEITDVAYNSKTVELFDFLNLQLLSLPKKTFNISTVTNVKFEFNDVNTTSSSVSNRLYIYTTDEDTSFYPFIVSQKNVFESEKNYGSYDTVNGCYSIPFTIKKNSITGVYDYYIYVDPYTFIQGTVFAQMFGSLAELRVNSYYGDSLGPMIIDVMTETVPQVVSQNTYIGWNITIQDNINGFKVGFISFMSNVDLFKYQFRFDASNRYSGDKYLGNYQFLIPIDYRCRSQVFSIFKVYLEDEMGHISDYRVDRPNTINGFNPTMYFSNFTESTMQTTNCDGNGLVNPSFPKLKSFDFNPKQVNTYSTQADRLVKFSFTTFHESGILLESKPIIYIQDNNINFVSEKSTLVSFNETYAQYSAAIALPYGFGGSNNLTVSIFGIIANQGVIFGFNPTDLLSSSFPYSISVTSAIQTSNILISKTDPATSNGGQFFVYGNQFTNGDKLLVRPVDDAEFTLTPYYSKSSIMLVQLPPMGTATMMTIQVVRGQTVSNSFTVLISQPFKPTATCLNGCNGGGTCTVNGCVCFPLFTGTDCLTKILQVTPSFNTTHPQIDVQVSTTIKSQLEFIGLAEVDSNKNIVKQFNIDKWTFLETSSSERSYQGEITHSNSNSKTLILVNTSFNNKNVTTPVYFPSYNFNLNPYAFKYTITLGPYPFELPSNSLQLIFSSTLQGKDLRDTNQCSLKEITSDVRAGFTLTRLKLEDHVLLGRYLNRGVGSPIQVVPIQNYQVDSRFKSLADNSFTRKIYMAVTMPSYSTSAAIDPDFSYLLENTKASSSQPGAICYEVPPVLPTNPPQECLNQCSGNGQCSTQGCICTLPWTGLDCSSKIVVISKPPTLNSTNPETTIILPKEENESEKKPEFYAIISVIGLNELDKDGTILFQYPLNKWTVVDKESQYAQFTNSTYHYMTEITNKFDQSNTTLNVSIDYFDLPEAVNITFANQVLTMNPYSLKYSIDISKYSFSSSLNTLQLVVTASLESTKSDCSLLQTGNTVESESEFVKMQVNDHSLYGRFIKRGVIDGRIKAVSNTPLDTQYNSISTSSKSQTYIGINIPFYVNSAQLDPDFSVLVDTRSASGNTNAICSKSDSKNSLTTAQIAGIVIGGVVFLFIVGALIIYFMSKKGNSKMALKLRKIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVJ4</accession>
    <name>A0A8J4PVJ4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Striatin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_008265</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat striatin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000494">
        <property type="protein sequence ID" value="KAF2070421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVJ4"/>
    <dbReference type="OrthoDB" id="727118at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013258">
        <property type="entry name" value="Striatin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051488">
        <property type="entry name" value="WD_repeat_striatin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15653:SF0">
        <property type="entry name" value="CONNECTOR OF KINASE TO AP-1, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15653">
        <property type="entry name" value="STRIATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08232">
        <property type="entry name" value="Striatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Striatin N-terminal" evidence="9">
        <location>
            <begin position="18"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="444"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="498"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="569"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="623"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="696"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="89"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="204"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="280"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="347"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="417"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="37"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="108"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="281"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="293"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="357"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="377"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="417"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2070421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="775" mass="85998" checksum="5DA3B8D9DA281457" modified="2022-05-25" version="1">MDDHGNGVQQGPMHADMTMPKVLDFLQTEWFKYEVDRSHWVAEKADLKNRIIQLESEKKSNEQIKNDLCKRIKMLEYALQQERVKNSTLYNSSSSGTPQQQPQQRDNIAAKRDFPKSSRENMTRAYIKKYLKDIGYNDIIVTKPEQDDIYSQQQQKDITIEEPTIFNFSSPQSSNTVMLNTNNSSRYSPSTQEIEQQLETEMNGLLHGNNDNNNNKPPKTTTTTSTTATSPPTAVVGGELDTLKDLMQNTTEMDLSQSLSSLGSFDHDSLLDSLKELDEGVSNTTTPQDSLTNSNDSFKDNNSSSNNNNNNNDQYNSFNEEDFFNKLSANSKGRMKLKLGNIKGLKSQYDDQEDDNGSSSSGSASSGGSGKKKSSNGKKSSSSSSNNGGSIKGDSMKKKLINPDLLGLGTSDLNDITLDDSSSRSATGGADSTSPRTWRHKHSLKSHFDGVRSILFHPDEPLLLSASEDHTIKLWNLTHLTPSTKKGAAVEIEPLYTYRGHLGPVFTTALSQDNKSFFSAGYDMIIKQWTIPPMNLDPYDCHGKVINYLEREFVGHTDGIWDLVPFSPSPGVNQLLSSSADGSIALWDIMSSMSQLCTFIHPLGRDRIPTSISLPPSENYRKMLASYNDGSVLLWDLETGQVVQKVKQEMPRGCEEQVNKIVSHPFLPLAIMGTEDHKIEFWDLSAPANIVPVHSMIAHSNSISSLAIDPSGLYLSSCSHDSSIRFWDISSKTCTQDLLAHRPKFDEAIHCISYHPTKGYFASGGADAIVKLNYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVL3</accession>
    <name>A0A8J4PVL3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000150">
        <property type="protein sequence ID" value="KAF2074376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVL3"/>
    <dbReference type="OrthoDB" id="429143at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF281">
        <property type="entry name" value="FAD DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="1">
        <location>
            <begin position="14"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="385" mass="43068" checksum="3AA0256F55B27150" modified="2022-05-25" version="1">MTSFWEKESFLNYDYIIIGSGIVGLSTAISIKESKGDGASVLVMEREIIPTGASTKNAGFACIGSLTEILDDLKTMSEKEVVELVKTRLDGLRLLRKRLGDDNIGYKENGSYELISKDQPYKELANKVDEINALLYPVLECNAFTICPQEEIDRRQFDRDHIKLIIKNNLEGEIHTGKMMKSYLALANKMGVEIKNGCDVSSFKDYNTHVEIEIQNKAAGKQLFTCQKLAICTNAFTKSLVPDIEDLVPGRGQVCITEPIPDLPFKGVHHFDEGYYYFRETEGRILFGGGRNMDFKGEETTEFEFNEKIQNQLVHLLKTVIIPNRPFTIASKWTGIMAFGSTKKPIIKNYSKNVVLGVRMGGMGVAIGSLVGQKVRNLLLLNSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVQ5</accession>
    <name>A0A8J4PVQ5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cytosol aminopeptidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_004936</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Release of N-terminal proline from a peptide.</text>
            <dbReference type="EC" id="3.4.11.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.</text>
            <dbReference type="EC" id="3.4.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000184">
        <property type="protein sequence ID" value="KAF2073767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVQ5"/>
    <dbReference type="OrthoDB" id="412814at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00433">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00181">
        <property type="entry name" value="Cytosol_peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011356">
        <property type="entry name" value="Leucine_aapep/pepB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000819">
        <property type="entry name" value="Peptidase_M17_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023042">
        <property type="entry name" value="Peptidase_M17_leu_NH2_pept"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008283">
        <property type="entry name" value="Peptidase_M17_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963:SF23">
        <property type="entry name" value="CYTOSOL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963">
        <property type="entry name" value="LEUCINE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00883">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02789">
        <property type="entry name" value="Peptidase_M17_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00481">
        <property type="entry name" value="LAMNOPPTDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00631">
        <property type="entry name" value="CYTOSOL_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytosol aminopeptidase" evidence="7">
        <location>
            <begin position="336"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2073767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="489" mass="52448" checksum="DEC549FAA68F2EB7" modified="2022-05-25" version="1">MSSVSDKGLVVGFYENSEELTKEAQELNTKTNGHILKSIQLSDTKGKLGDTLVLYDITPEVPRLAVVGLGKKSDVPSGDYEKFENVRRVIGSGVKSLKAKGASEITIDSTLGDVRSISEGAHLSKFKFDFKTKKADATPEKNVNIALSDASLEASPEWSTGKVYADAQNFARMLMETPANLMTPSIFVSTVQDKFKDLVASGKVEIVVRDEAWVLEQKMGMFYGVAKGSDEPLKFLEIHYKGAADATKPPVVYVGKGVTFDSGGISIKPSAGMGLMRGDMGGAATTVSAIYGAASVGLPLNMIVLTPLCENMPSGKATKPGDVVYAMNGKSVEVDNTDAEGRLILGDALVYAHTFNPTTIVDVATLTGAIDVALGQHFAGAFAATDCLWKQIEQSGNTAGERMWRMPLAQEYRKQMDSKYADIVNAGGRSGGSCSAAMFLKEFVTVDRWAHLDIAGVMYSNEDGAYLKRGMTGKPVRTLIELAREISKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVR4</accession>
    <name>A0A8J4PVR4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_003795</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ku70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000123">
        <property type="protein sequence ID" value="KAF2074918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVR4"/>
    <dbReference type="OrthoDB" id="3249161at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043564">
        <property type="term" value="C:Ku70:Ku80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00788">
        <property type="entry name" value="KU70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01458">
        <property type="entry name" value="vWA_ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.290.10:FF:000001">
        <property type="entry name" value="X-ray repair cross complementing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000080">
        <property type="entry name" value="X-ray repair-complementing defective repair in Chinese hamster cells 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.970.10">
        <property type="entry name" value="Ku70, bridge and pillars"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019406">
        <property type="entry name" value="APLF_PBZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006164">
        <property type="entry name" value="DNA_bd_Ku70/Ku80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006165">
        <property type="entry name" value="Ku70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027388">
        <property type="entry name" value="Ku70_bridge/pillars_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047087">
        <property type="entry name" value="KU70_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005160">
        <property type="entry name" value="Ku_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005161">
        <property type="entry name" value="Ku_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016194">
        <property type="entry name" value="SPOC-like_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00578">
        <property type="entry name" value="ku70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604">
        <property type="entry name" value="KU AUTOANTIGEN DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604:SF2">
        <property type="entry name" value="X-RAY REPAIR CROSS-COMPLEMENTING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02735">
        <property type="entry name" value="Ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03730">
        <property type="entry name" value="Ku_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03731">
        <property type="entry name" value="Ku_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10283">
        <property type="entry name" value="zf-CCHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00559">
        <property type="entry name" value="Ku78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100939">
        <property type="entry name" value="SPOC domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ku" evidence="15">
        <location>
            <begin position="360"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="674"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="41"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="674"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="694"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="705"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="719"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="739"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="758"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="850"/>
            <end position="860"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2074918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="860" mass="96454" checksum="B097AEDCB0BE9149" modified="2022-05-25" version="1">MSGSNGIWGSNTFSAVNTGGGGGTSGGTYSYMNQQQFNNDEWDNIFDNNGDDDENNDNNNNNNNYNPYSVRDAIIFLIDCSTSMFEPNENKEIPFNNAIKCLIQTITDKIITSDSDLIGICLYNTEKKKNINDFENIYVLFDLDVPDPKIILSLEEMLETDFSKSLGHCPTEFPFCDALWTCSSMFSNCSVKQQMTKRIFLFTNEDNPNGFNDSIRNVSLQRAKDLSDLGIQIELFSMNKPDQTFDFSLFYQYIIVFGADEYIDANQFDASTRFSDLKNKLKRKEFKKRSLGKIPLYIGGGSNSNKKSGSTQADANNQIIIATQLYNLVSTATKSSPTQLDPKTNLPVKVLRKNICLDTGATLLPSQIKYCYYYGGEPIIFSKDELEEIKALDRLGLVLLGFKPTASAIKPYQNTKHASFLFPDESSIKGSTVAFNALVQQMIASDKVAICRFTARSNVSPRMVALVPQEEVLSDDGMQIRPRGMHIIYLPYADDIRSCTMTSTSTCKDKPDYVDKAKKILKSLKIKFDDKKYLNPNLQKHYSNLHALALERDKVEPTVDAIFPDIQAAPSLIEEFTNTVFTEGYAERVANSSTGVGSKRSRDSGKDYSTLNWQDLAKTGNIAKLTVPEITLFLKNQGVKIPTKSKKGDLVDIAIKYINESGIKKDKLLLLANNNSNGNHDENNNNGKKTTKKSNKDEPMKGNIDDEDFDFGNDDDGDINNNNNNNNNNNNNNNKMQDDDVNDNEFDDDFRPTKKNKNNTSPPNSQPKKTTTTTTTTTSSNNNGKKKSNTTAKIVNDDEDDDFLPQKQKNKNSDDESTDDEKPKDKGKGPRPNCPFDGKCYRMSNPQHTSKFRHIKQQSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW02</accession>
    <name>A0A8J4PW02_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002632</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000074">
        <property type="protein sequence ID" value="KAF2076068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW02"/>
    <dbReference type="OrthoDB" id="10624634at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042752">
        <property type="term" value="P:regulation of circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026211">
        <property type="entry name" value="GIGANTEA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36319">
        <property type="entry name" value="PROTEIN GIGANTEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36319:SF1">
        <property type="entry name" value="PROTEIN GIGANTEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="709"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="980"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="54"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1120" mass="128359" checksum="1546F5E5048D3CFA" modified="2022-05-25" version="1">MSDNNNNNNNNNNSNSYTPPHYHHYNNYQPQQQYNSPYDPSKKKKKTYHGIDYNSNSSSNTGSNNSGGLAQNSKNRQAITNNLLYSDALNIKITVHQPIPTDILTKVINQWLNTVDREKTTSDSKPSDQFEYNLVSLIELSYPYDSKTLLDYVITTFCVYRPEYIGVIVKTLIEQIYSGILVYRFNMPPLFAFKQLFSDKKNSLFSNTDTDQQQQPQQHYHHHHHYNNYQQHQQQQKQNVFELNNQIDELDDSNDIPLQILFEITSYNIIYHINNSNSKQPQQQSYIASHWILDCLVKSKLSIIETYFTQLLLVNTKNLSNHRYFRTLHSFIKSRSSDNNNSIDVSKEIRDHIVSMFNSAAPNSLPLDSKKIQPILHTIVPLLIQYQRKYISQDYLFIILYYFSSTTSSFVQRLFHNLISSVSFPTLHNSNNNNPIIDINQYNCINKQNVIIDIIDILKSKSKISNQLLCNNVVQPILQCINEGIFSNNAIELLKEIINSSNYNEILFGVNYEGFVFTESQLQQGYKSIQLLLNTLESYSSTTQTLFFQLWEEYLNTLFTSQSIVAISTSSSSSISSLFWRVRQMLLYFNRFPLELRDMTKRILYSFISIMLEKNRFDSKVVSIYSQLTHICFYLSSCANDESTDEERDNIDLFIEYIQFITTNFKPTHIIYTSVMKSIHQIISNITLIDSSYMLLGFKSSIDSSNSNSSAIGGNSSNNNPSSNSNTSNPINDLVKNSIFTLDIKTIIQSLYYILQILGSRNNSSNSDDLHQQQLNTQILLSNIISNDVNSKNNILQLLKSNDTNLKPISIKLLIEMETHSSSNVFWNQEKIQILFKHLMDPNLFPLCLEFLGIIKEKCCESVLKSLLRNLILSYDSSNSNGSYNQTTTTPSTYMGIIALIEEILVHSPPLTLIALQFIQSHLFTTTSATTSNNNNNNNNYSFDMSANNSGSSNNINDINNQNLLFLLQKIQSLVNTITQSSPPQQSSYTIITSSSSPTTTTTPDSVLNSIVLLMNEIILFSLKNIDDQNGDIQLLQFQLISSIVSTHSYEWHSNFIQEQLYIVVDGLVSQNTPIQNLCHTILKEFINHNPAHHKPLIDEYVEYRNLGKANAYPSIRTPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW26</accession>
    <name>A0A8J4PW26_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_004026</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000135">
        <property type="protein sequence ID" value="KAF2074676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW26"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057709">
        <property type="entry name" value="DUF7949"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25820">
        <property type="entry name" value="DUF7949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="806"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="5">
        <location>
            <begin position="108"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="4">
        <location>
            <begin position="261"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7949" evidence="6">
        <location>
            <begin position="516"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="3">
        <location>
            <begin position="568"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="477"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="477"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2074676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="854" mass="95875" checksum="1EBB326452D5AB83" modified="2022-05-25" version="1">MVYFENNYIDLTFSAQTNYLYFNFTTAGPDTTIYFVCHIYPEPVNYRDYPFYYNEILQLYVIQFTLPMKLLEQELDYSIWISSYEITSEAIQAKFTTNGTLHVLSESGDQFPPLIKSISAIQTPVSITYSATDTTPQSLGWIVKIDETTGLESANFTVTSDLDIVGYNFTFLPQDAISVDGITLTFSISFNMAPINCTKSHVYKISFASLRDITGRVSQFNAINYQLDPPTFLNPFYNFASAETSVTLTCLDTPTLDVTPPKLKSIMYYTPSEYLNTATQFRSLAVTFIFEDTFGILYKNLPVMFLQSFVADELQVIACETPTILSTSVLTRVTYTTNCKYPYGFGYPNHILISISGVFDTSYNLGYINNNYLDTNATDAALSLETFSTNQGYVFLYGYNMVIPNDPLFNCTFDLEFGESKLFGYAPTKFLIFEPVSIAFKIPDVIAPVGQFYLIIQSPRGKSRRVLIEFQPWVPESSLSQSSSSSSEPSSSSDHSSSLAPPTKAPETLPPVVNKCPGAPVCGGDGKGKCESIGCVCVPPYIGFDCSSLVIPIVVNPNTTTPNTNISIDIPTSQGSKEVTLSSLVQIISLREMSNDDIVYQHLFKEWVYTNISKQQPQYLYQTNITTNDGKTTAINVTVTYYEQQQFIIFAQQNITMNPNSFKYQVSLSQYSFQSHSNYLQLIMSTQFQSDEQDACSYKDFTDFNQDEYLIMQINNHIFNARFIKRALVDNRPTLITNQLINNDKYVSADTPKSSTSFIGINIPYYTRDVIIDPDFSILLSTDSAKDKKGSTCMQGNSSKLSGAKIAGIVVGSVVFVTAVVVSVTYHFYLKKKRADQLKRMNNKLERMNRNNSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW73</accession>
    <name>A0A8J4PW73_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000462">
        <property type="protein sequence ID" value="KAF2070661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW73"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="225"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="726" mass="85764" checksum="38FA34C4752EDB9A" modified="2022-05-25" version="1">MYQYYQQIFNHKYLSTQIFKHVHQIQCNHYSLKYDEIVDIGWMHKYNHKGLLKEKLNNSNNSNNNLYIKPKYLFTAIANNDTELFISLFERYKYLTLDYYLFHSDEILSILNNIEVVKYLFQRGYARQVYEIKIDKVDRDIIFFYIDNKWLKPTISIFSQYIRELEQENQTARTDLQDSIQLIVKHLENPNLNHLDFFKLVRYPIPSLIQQLLPLLQQKYHQESLEIESSQLKILIDNIQSQEDELVRTKESNSVAVVAAKADLFKLMKEGLLVDPLISVWCEWVVKGDEEFFGLWNRLSKRLRVSSNHIYFNTTTDIDASTSDCQLRRDSSVLDIGRIVIDSCCVNTSVQVLKFLYQQGYNLVNRVRNNTAFFKTLVLLTGNQDRETIVELANSTQNIILPKIRILECCCRAKHRENLDYYLGKFKADINDQQIKRLFKLCNKKPYLIKLLTSHGLQWPSASVGDLVLYNYCWFEKFSAERLEKAFKSIVFTTKNQAEWERREMFLNSLLVHSINNNDYPSFKHLIINARMTLDVKDQDIQISLALCTNLNIIDYVNKNRSTCFSISLSALVVNKFFADIFEKSFDQESFNPLLIDYMIKQNAINTKTLSTLQTNLLESRTQLDKVQFTPKQEITFNYFVYFVQEYKHDSIDLLCSVLIQSSQFVEYIYRNQNRFTSTPRFQSIYDYIIQNFLGSSSFGYYLHLIKDLINRYDCIDTSGIARFDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW74</accession>
    <name>A0A8J4PW74_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_003845</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000126">
        <property type="protein sequence ID" value="KAF2074857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW74"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042805">
        <property type="term" value="F:actinin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008307">
        <property type="term" value="F:structural constituent of muscle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000001">
        <property type="entry name" value="Cysteine and glycine-rich protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24215:SF35">
        <property type="entry name" value="MUSCLE LIM PROTEIN MLP84B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24215">
        <property type="entry name" value="RHO-GTPASE-ACTIVATING PROTEIN LRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="8">
        <location>
            <begin position="9"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2074857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="89" mass="9491" checksum="476D632B2BE255B4" modified="2022-05-25" version="1">MPPKFGSSEKCGSCAKSVYAAEKSPIMLGEKSFHKACMLCCECRKGLDSTNICENESKLYCKACYGKNFGPRIFGYSGGGVLGNTSANV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWL8</accession>
    <name>A0A8J4PWL8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_007845</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000441">
        <property type="protein sequence ID" value="KAF2070841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWL8"/>
    <dbReference type="OrthoDB" id="2161780at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016830">
        <property type="term" value="F:carbon-carbon lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050477">
        <property type="entry name" value="GrpII_AminoAcid_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42735">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42735:SF14">
        <property type="entry name" value="PYRIDOXAL PHOSPHATE-DEPENDENT DECARBOXYLASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2070841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="742" mass="82057" checksum="F93EB50F0F8D14CE" modified="2022-05-25" version="1">MYNIPANLKPTIKSFDLPPLQGETKEFAPQAWFLGPKSENRDLLAELVTSGLKRHVDGRLLFHPEDAPVFDEDFKKTPAYLNATDDMKKKTDQLLTFLQKSLPFSSLRYVGHMCSDVTIAAAVGYISTILYNPNNVTIQASPVTSYIEMAVGNDLCRMVGFEFPVTKSTDKKKKKQLKDDAIYPSCHLTSGGTVANCEAVWASYWAKFVPLALQEALIHESDLAAAKDITVKLCNGIPKKLSLLTSWEGVNIPADECVALPYRIADLCKLDPSKVTFICKQYHPSNVGLRKFLNKHELNDPVLFASASGHYSLPKAAALLGLGSDSLKLIPVDLHTRMDTTNLRAQLQACISHHIPIINVTCVQGSTEESSVDDFATVVKFRKEFNLKGLEFFTHLDYAWGGYYLSCIRDDYELENPETDVYKPQPAKKVATPFNSTNNLDPKYFCSNYFEAQMEAAKHCDTMTIDPHKAGYIPYPAGSVLYRNKRFRDILVFTATYIGGTNSPGAVGIYGIEGSKPGAAAAAVYLSHAIVRTSKSGYGQILKRTLLNTKLFYLRLLWLVGATDNFTVTGIPIPPPQAQLDSLKARLIVNGVMISSEEICKDLALVQELAEIGPDQNIICYTFNFKTPAGVINTDYQKLLTFNQKVYERFDYVPETGMKEFDVIVSNTDFLDHYGTPFLTNLASRIGVTNAPAVGVAIPAIRSTIMDPFANETTQGSAFDKVMSIFKTEITKIAASMVQESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWM1</accession>
    <name>A0A8J4PWM1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003673</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000118">
        <property type="protein sequence ID" value="KAF2075021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWM1"/>
    <dbReference type="OrthoDB" id="515366at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06467">
        <property type="entry name" value="p23_NUDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037898">
        <property type="entry name" value="NudC_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12356">
        <property type="entry name" value="NUCLEAR MOVEMENT PROTEIN NUDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12356:SF18">
        <property type="entry name" value="NUDC DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="2">
        <location>
            <begin position="14"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="158" mass="18242" checksum="C388B5D532CA03AB" modified="2022-05-25" version="1">MEKFKHNRRKFEYKGQTIYEWEQSIDEVNIFITPPPGVTSKMIACDIQAKRLALGIKGNSPFINEEFYSTVKVQDSFWTFEDGVIHITLTKMQKTETWLAALKGHDNQNGKESELNEEDKKKIMLERFQQEHPGFDFSGAQFSGQSPDPQTFLGGMKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWS0</accession>
    <name>A0A8J4PWS0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">COP9 signalosome complex subunit 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003209</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the peptidase M67A family. CSN6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000098">
        <property type="protein sequence ID" value="KAF2075473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWS0"/>
    <dbReference type="OrthoDB" id="1378at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000338">
        <property type="term" value="P:protein deneddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08063">
        <property type="entry name" value="MPN_CSN6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024969">
        <property type="entry name" value="EIF3F/CSN6-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033859">
        <property type="entry name" value="MPN_CSN6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540:SF8">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13012">
        <property type="entry name" value="MitMem_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="MPN" evidence="3">
        <location>
            <begin position="30"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU367006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="317" mass="35647" checksum="14E3B5BEEAD38533" modified="2022-05-25" version="1">MTESNTAATATNKDNPFVIDKGSSTSGLEVDLHPLVIINISDHYTRNKVESNNNNPTRVMGVILGVQNGRNVEICNSFEAVYTTVDNTIVLDLDYLKKKQEQFKKVFPTYELLGLYTTGHQVTSEDILLHKQIIELNESPLYLMLDTAAVLSEATKDLPIVIYESELHIVNDSPTTLFVKTNYKIQTGEAERIGVNHIAKVTPSGSEGSGLSTHLYSMHNAITMMNMRVKVLSQYLTSVKEKKVPFEHAVLRQIASLCNQLPAINTEEFNQSYLQEYNDVLLVTYLASITKTSSLLNETIDKYLISHERQGKRRPFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXP1</accession>
    <name>A0A8J4PXP1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002953</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000088">
        <property type="protein sequence ID" value="KAF2075711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXP1"/>
    <dbReference type="OrthoDB" id="23871at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32142:SF55">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32142">
        <property type="entry name" value="B BOX-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2075711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="685" mass="80776" checksum="5C765A5A78C8F716" modified="2022-05-25" version="1">MKYSSYQSVFNNVYLSSKIFATVHQIQRYRNSLKYDDITKVKWMYENNHICLLREKINRNIELHVDHRHLYGKIANTHPDIFIQLFESNRDRAVELIEYGMHTIENAEVIKYLVERGFGVETVIDRFEKLYKIELQAIQYLLENKLCPPPNFRLLLAFGDMRPIYNPTKARETKEKIEFVIQNVLQLPLTFQDSRSIVKSLLSDPTPLVFDAIEPLLDLGVENILYYHSRKNIYCSYREAVEDIRLVESEIFGGEKSVVERSDYPPHLKEWYACVGKDEQEFINTWNSLEHIIVVDDDIVFSESPIGERLYHIVSLISKSLRLVHFLISKGFTGLSKIQTSVKHDICRPTLKHHLQDISQQDRDLIKTMGRGDLQFINLVLSTFTQGYFESFNFFFKQFTQEFDSEQIHALLKSLFDIAAENRNLFLYESIEAMGYPFTDTMTSYTEFKSLLPRFGRDIDSWAEQQKELKCSTLLVSFIGGSNLIGFKYVFTKHKFSTLMHDNLLEGLAKCRNLVFIDHFWKNRSTCFIETPSTDEINTFFTAIFNRASANQNMLLLKYLVKNNCFNPNATKIFQEPYFSCKEPIDPKYDFKVLVHFVENRFFDSIDPYVKYLIDPTSICSHTFVEYLYRNQDKFNTTVSFQSMYDQMRFKIVDRQQLGIFHLKYHNQLMRTFVNRYGCQETKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXR4</accession>
    <name>A0A8J4PXR4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein phosphatase inhibitor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003419</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000106">
        <property type="protein sequence ID" value="KAF2075290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXR4"/>
    <dbReference type="OrthoDB" id="551302at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007062">
        <property type="entry name" value="PPI-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12398:SF20">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY INHIBITOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12398">
        <property type="entry name" value="PROTEIN PHOSPHATASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04979">
        <property type="entry name" value="IPP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="199"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="289" mass="33214" checksum="62BF109BF1803163" modified="2022-05-25" version="1">MNKTQQDNKDQEFEDIVENNNANGNGDDEDGDDEIDYDLYDVDGDDDMDSDEEEEEVYTKSNKRTNKTLSSNKSNSKKVGKNNSSSNSGGSSNKTSPFKIIPYSTIQANSPSKGILKNRNAIPPTKRRIVWDEENLNINESQKSATMKIDEPKTPYHYYESEEENDDSKKYLENKFLELEKALNRTKSEWDSDEDDSQGSDSDSNSKKTTLTTTTTTSTNKNNNQNKKKHIKVNLRANESDDDDEVEEEEEMDEDKKESKKKFDKLRKSHYNEFLTLKNLKTDISDEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PY54</accession>
    <name>A0A8J4PY54_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Calcium uniporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_001462</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Mitochondrion inner membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">The selectivity filter, in which calcium ions are arranged in single file, is composed of two acidic rings separated by one helical turn along the central axis of the channel pore.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the MCU (TC 1.A.77) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000035">
        <property type="protein sequence ID" value="KAF2077208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PY54"/>
    <dbReference type="OrthoDB" id="278338at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990246">
        <property type="term" value="C:uniplex complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015292">
        <property type="term" value="F:uniporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036444">
        <property type="term" value="P:calcium import into the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051560">
        <property type="term" value="P:mitochondrial calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006769">
        <property type="entry name" value="MCU_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039055">
        <property type="entry name" value="MCU_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13462">
        <property type="entry name" value="CALCIUM UNIPORTER PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13462:SF10">
        <property type="entry name" value="CALCIUM UNIPORTER PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04678">
        <property type="entry name" value="MCU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 15" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4 15" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13 15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 15" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Calcium uniporter protein C-terminal" evidence="16">
        <location>
            <begin position="90"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2077208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="296" mass="33847" checksum="E1964716B338EB34" modified="2022-05-25" version="1">MNVLYRGTLRMATMPFNVSRVGASQTGFLSSNKKMFSTNQNLSNELQTILKQGKVVKLQERLNLDPRNKIPVTEFKSICTEFGIPENEVQSLSSALSQTGSIIYLPTSSSKDLQSTVFIKPGHIYQSLYHILDLENKGVGLDKLIEAKKNEIATLKQTIKPLEEKKQIIDAKAHRRATIIIYSGLGYCFIQAAALGRLTWWDLSWDIIEPVSYFLTFATVLIGYTYFTITKTEFSYEALNNRLFTKRQSKLMKKYDFPLEEYTRIKNLIEKREKEVEGLESAANYSSADLEFNFNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PY99</accession>
    <name>A0A8J4PY99_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_004407</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFB3 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000155">
        <property type="protein sequence ID" value="KAF2074274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PY99"/>
    <dbReference type="OrthoDB" id="15795at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012576">
        <property type="entry name" value="NDUFB3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08122">
        <property type="entry name" value="NDUF_B12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2074274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="203" mass="23256" checksum="2DFE08EDD8D04B06" modified="2022-05-25" version="1">MFKDYNNIQFKSPFLKKLENLKKLYLLPMKIDPDTIPNSVTRLQLNICGTTYGLPRNIIPPNVVDLTLFTNDNNTLELATFPEGLKRLTFAGVNRSLNFSDLPKGLEYLDFGSLYQYQYNVTDLPNILHIVNLPLIHAQDIQIPQDKYKMIFGKINEWRSNPAYKPRFNTVVPGFLPAVGLFTAYCIIEAAYNSMTGPKKDHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYF0</accession>
    <name>A0A8J4PYF0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Short-chain dehydrogenase/reductase SDR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001252</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000029">
        <property type="protein sequence ID" value="KAF2077403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYF0"/>
    <dbReference type="OrthoDB" id="16849at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="55" mass="5858" checksum="FA4E4296941D9894" modified="2022-05-25" version="1" fragment="single">MDPKQIIKPIALISGANKGIGLETAKSLVKKGYFVFIGARDTVLGEKARDEIGAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYK0</accession>
    <name>A0A8J4PYK0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_002347</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000064">
        <property type="protein sequence ID" value="KAF2076341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYK0"/>
    <dbReference type="OrthoDB" id="2158884at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07830">
        <property type="entry name" value="STKc_MAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000545">
        <property type="entry name" value="CMGC family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000104">
        <property type="entry name" value="serine/threonine-protein kinase MAK isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="153"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="75"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="465"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="75"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="465"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="536"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="548"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="606"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2076341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="615" mass="69240" checksum="97D034D705291A0B" modified="2022-05-25" version="1">MSTPTPTLHSSLASPSTSPPLITTSTSSTNTASLQQQQQQYLSSSNHAIHMIAGGTSIQSLNEIHSLSISNNSIINTSQSPSPSPSLSSSAPSLSQFQPQQQQKEKDKSALIEEYNILKQKIQQQKQQKQQQQQQQQQKEKEQEPKESLMDRYIILKQIGDGAYGDVVKAYHKVTKEIVAIKRMKRKFNTYGDCCQLRELRSLNQLNHPNIVDLLGVIFENNELFLIFEYLENNLYETIKDRTKLLPESTIRNIIYQMLQALYYMHSTGYFHRDLKPENILMMDDTLKIADFGLAREINSKPPFTDYISTRWYRAPEVLLRCSFYNSPIDMWAIGAMMAELYSLKPLFPGTSEIDQLFKICSVLGTPNHHTWSDGMKLAQSMNFIFPNMPATNLATLLPNANPDAIDLLTELLRYDPLKRPSALQALQHRYFMVSIPTQILLKTDYSQLANSHLIKNGYIKDENLNGNSSNSNNNNSQSNSTNTNSSTNNIIANTTNNNSSNSSSHNRNNSNNNLKVSVNNVNTTTNQQQQKSNPFLRNSRYSLKNCNSNNINRTTTTTTTTTTSTISNSSVDSNISDNNSNNDSNNNNSNSNSNSNSTTITNSRNKIRKPRNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZB4</accession>
    <name>A0A8J4PZB4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_002461</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000068">
        <property type="protein sequence ID" value="KAF2076220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZB4"/>
    <dbReference type="OrthoDB" id="438495at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046964">
        <property type="term" value="F:3'-phosphoadenosine 5'-phosphosulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013657">
        <property type="entry name" value="SCL35B1-4/HUT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778:SF8">
        <property type="entry name" value="ADENOSINE 3'-PHOSPHO 5'-PHOSPHOSULFATE TRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08449">
        <property type="entry name" value="UAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="267"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="344"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="374"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="398"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="428"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="467"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="493"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="519"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="120"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="120"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2076220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="553" mass="63685" checksum="32EAD081E6143857" modified="2022-05-25" version="1">MDVDILFDDFPNKSSTPTTSNNNSNNNNNNKASPTSSFFIDRHLPQYQQLLQQQQQQNIYHDYITRIQSNIRYTPTPDTNSALVSEQNYNSNYSPKFFTNEKNNNNDILIQQEQQQNINVVGNNSNNNNNNNNDDDNLKSPYSKSLLPNNSQVSILQSFLSKFEKTIQLDSSTSDSNHFKRKLKNAASYVLSLLPISTNKYTKLPPQDIISTINNNNNSNITSTTTTTHDDSKSIKNIVFCTFYVVTLYLIYGLLQEILFKKQKVNFFALYSFAQFFVSFLLSVRDIYIKQKSNTQDGNVTHLQLFQRLSLKKIKLYSLLSLILFFTKLLGNEALRYLNFKTKILFQTSKIIPVMIIGGILFKRTYNKKEYMSIFFMISGLLLFCIGESFSSSLMSPLALFLILAYIFVESFKSILYEKILKDFSSEVELSLFTNFFGSVMTLPILFVSKEIKTSILFLIDNKLTLLIFLAFISLGYFANIAYLNLIKITDSFYANMISSFRKFLTILLSFVYFHDSLLFYHAIGILVFFGGLILEIITKSKETSEKRNNKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZN8</accession>
    <name>A0A8J4PZN8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Rho GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_003452</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Lipid-anchor</topology>
            <orientation evidence="10">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000108">
        <property type="protein sequence ID" value="KAF2075234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZN8"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00157">
        <property type="entry name" value="Rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001799">
        <property type="entry name" value="Rac2 family GTP-binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="171"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="244"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="244"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="280"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2075234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="327" mass="36094" checksum="A50794D8819D7A7C" modified="2022-05-25" version="1">MSSPPTTTTTTTTTTTATSNTTSNNTPPPPIEDKSADRIKKRDPGGITKVIKCGVVGDGTVGKTTLLLSYITQAFITEYTPTVFDNFSAIEEVDDGKLVNVILWDTAGQDDYAQIRTTCYTNCRYDVFLLCFSCVNRDSFDNVKYKWLPELKANSPGTPFILVGTKTDMRDESEKQQANSPPLTGAINPNISSKEGQKRAKEIKAHSYMECTSRDPASVAKVVLEAINIIIEKERLKRVNNEKQWKKEVKKEEKEEKSFLKAREKYEQKQKSKEEKKKPNSPNSNSPPSTPEQSSPQSSKKSASSSTSTSPSTTPNTTTATTVTNGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q009</accession>
    <name>A0A8J4Q009_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Vasodilator-stimulated phosphoprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_002489</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Ena/VASP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000069">
        <property type="protein sequence ID" value="KAF2076189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q009"/>
    <dbReference type="OrthoDB" id="31170at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22062">
        <property type="entry name" value="WH2_DdVASP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038023">
        <property type="entry name" value="VASP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014885">
        <property type="entry name" value="VASP_tetra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000697">
        <property type="entry name" value="WH1/EVH1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202:SF22">
        <property type="entry name" value="PROTEIN ENABLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202">
        <property type="entry name" value="SPROUTY-RELATED, EVH1 DOMAIN-CONTAINING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08776">
        <property type="entry name" value="VASP_tetra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00568">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00461">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118370">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein, VASP, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50229">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WH1" evidence="9">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="WH2" evidence="10">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="107"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="115"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="132"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="148"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="217"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="291"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2076189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="367" mass="37531" checksum="B8AD5570C16D1335" modified="2022-05-25" version="1">MSEVCIFNATGQVFTYSAQTRNWVPSSNVPATLQMYHNTAANTYRVIGRAGDDPNNFLINFSVKADVVYNRSTETFHHFSDQRTYFGINFTSKEDADRFGGGFENLLKTIKGGGAPPPAVPPPAKPQPVQPVAPVQRPASTTIAKPAAPAPAAPVAPAAPAPPAPPAPPKPPAGGPPPPPPPPAAKPPSAGGRSALLGSIEGFSKGALKKTTTIDKSAPTLSSKPAAAAPSSAGSDSTPSAGGGAAPAPAPKFGGGGGDLMAEVMAKRAKMKAAASAPKEDAPAPTPAPAPVAKEPTPAPSFNKPSSFNKPKPPPAAGRPSLSPKDTTSPPSVLPEDLQSLKDEILTEVRKEIQKMKDEILDAIRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q044</accession>
    <name>A0A8J4Q044_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADH:ubiquinone reductase (non-electrogenic)</fullName>
            <ecNumber evidence="2">1.6.5.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_000203</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a quinone + NADH + H(+) = a quinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:46160"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a ubiquinone + NADH + H(+) = a ubiquinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:23152"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.9" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000004">
        <property type="protein sequence ID" value="KAF2078453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q044"/>
    <dbReference type="OrthoDB" id="3244603at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) (non-electrogenic) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045024">
        <property type="entry name" value="NDH-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054585">
        <property type="entry name" value="NDH2-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706:SF47">
        <property type="entry name" value="EXTERNAL NADH-UBIQUINONE OXIDOREDUCTASE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22366">
        <property type="entry name" value="NDH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="12">
        <location>
            <begin position="111"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="External alternative NADH-ubiquinone oxidoreductase-like C-terminal" evidence="13">
        <location>
            <begin position="508"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="32"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF22366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2078453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="575" mass="65034" checksum="C89FD6AB74DF1D44" modified="2022-05-25" version="1">MMISRNLSKNIFGIKHQTSKIAQTSLNRNIRSYSTENNSNNSNNSNNNNEEKQKQQEPKKKWNKWLVFGGLAAAGFGAFTLTDLIVNDDFDSITDKFRSKLPEAERKHRPKVVILGTGWASLCMLRKLHTDEFDVYIISPRNYFLFTPLLVGGTTGTVEVRSIMEPIRKYCKRSDSEEATFIEAECVSVDPKKKTVRCVDNSTVKGEVSEFDLQYDHLIVGVGADNQTFGIPGVKENACFLKEINDTRVIRDKIIDCLETASYPGQPEKEIDRLLNFIVVGGGPSGVEFTAELNDFLQSDLLRTYPLAKRIKVTLVEALPHILTIFDKKIIDHVEKRLTSSDNTRIWTKTAVVGVKEKEITVRNDKKEESQVPYGLLVWATGNTPRALTTQLIKDIGPSHQNNRRGLIVDEYFRVVGTDGIWAIGDACINLQKPLAQTAQVASQQGRYLGRLFNHMAEDMNKQKIENAKSLSSAIVTTVSTTAATPLFKENQDFNDKLKNQPIFKYRHMGTLAYIGDHQAVAEFKGDSTTTFEGYFTYYLWRSVYFTKLLSMRNRVLVSFDWLKSTVFGRDVSRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0F8</accession>
    <name>A0A8J4Q0F8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cullin-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_006273</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11 12">Belongs to the cullin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000282">
        <property type="protein sequence ID" value="KAF2072416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0F8"/>
    <dbReference type="OrthoDB" id="27073at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902531">
        <property type="term" value="P:regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000014">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000007">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000011">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000026">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.130:FF:000003">
        <property type="entry name" value="Cullin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000029">
        <property type="entry name" value="Cullin homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016157">
        <property type="entry name" value="Cullin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10557">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01256">
        <property type="entry name" value="CULLIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cullin family profile" evidence="13">
        <location>
            <begin position="407"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2072416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="769" mass="88956" checksum="A74468F78B086256" modified="2022-05-25" version="1">MSMMTSAPQKSVKLEDIWPDLEEGIYKIITDLNKGFPRNKWMALYTHVYDYCAASQSKGSTGKTGMPKQQSTGANYVGEELYNRLNLFLKNHMKELLQVTDSKMDETLLNYYYTEWDRYTSAMKYINNIFQYMNRYWIKREIDDGKKEVYEIFILSLVIWRDYLFTPLKIRLTSSLLDIIESERNGYQINTHLIKGVINGYVSLGLNREKPKETILQVYKSSFEELFLTATENYYTNESAKFISEHSVADYMKKVETRLNEEVKRVQQYLHPSTETELISKCEKVLIEKHVEVVWNEFQSLLENDKISDLTRMYSLLSRIPRGLEPLRATLEKHVQNVGLQAVSSIANGAIEPRVYIETLLKVFKKYNDLVTGAFRSDTGFVASLDKACRRFINENAVTLAAKSSSKSPELLARFTDFLLKKSPNNPEESEMEQLLNDVMIVFKYIEDKDVFQDFYSKMLAKRLIHGTSTSEDLEGTMIGKLKSTCGYEYTSKLQRMFTDMSLSRDLLDRFNTHLEQVERSQLGIDFSVLVLATGSWPLQPPSTNFSIPKELQACEELFQKFYQVQYSGRKLNWLHHLSKGELKTKYLQSSKSGYTLQCSTYQIGVLLQFNIYDSLTTEEIQGSTQLIDSVLKGTLTSLCKSKILLSDPALDDDDISKSTKFTLNKGFKNKKTKIFINVPVLTQVKEEIDSIHKTVEEDRKLQIQAAIVRIMKMRKQLPHSGLMTEVISQLQTRFNPKVNIIKKCIDILIEKEYLQRVEGQKDMYSYVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0K1</accession>
    <name>A0A8J4Q0K1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">O-methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_001159</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000026">
        <property type="protein sequence ID" value="KAF2077541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0K1"/>
    <dbReference type="OrthoDB" id="16076at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712:SF2">
        <property type="entry name" value="O-METHYLTRANSFERASE CICE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_4G14580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="5">
        <location>
            <begin position="16"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="4">
        <location>
            <begin position="113"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="5">
        <location>
            <begin position="400"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="4">
        <location>
            <begin position="499"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2077541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="643" mass="73099" checksum="B87E45D9D8351CB7" modified="2022-05-25" version="1">MISKDCRIENENSLFDLATGHWKSRALAFIVENKIANYLIGGPKSAFVIADEIKVNGDFLYRLMRALTTLDIFIEEKEFGVFSQTPKSQILCEDQGFMDNLTFELHDFSYLAFSNLRETIKEGKTLGPNAIGCDSVWEAIKQPSYTTLFQKAMTSYTKSSIEIILSNTNFSKFNTIVDLGGSQGVFCLEILKNNPSIGKAINFDIPLTIQSNKSLLKSRLKSYESELSRFEEIQGSFFDANTIPEADCYILKLVLHDWSDDQAVEILKAISKSMKPTSKIYIFDTILERNNEMFNRYSWMDLQMVHYVGGKERSIREWSLLSKLADFNINSIQTLPGVFRCTKGDFRNTKSLSCMWRVKSSQVLIQQIPTFSNNEGVNFLLNNIFKIKGWDVEKEKTLLLDLATGHWKSRALGFIVEYNISSFLIDGPKSAYEIADEIKVNGDFLYRLMRALSTEEIFKEESEGKFSQTPKSQILYKDEGFRDFLIYQLSDFAYSACSNLKDSIKEGKTLGPKAIGCNSFWDVVQTPSRMPLFQKAMTSYTKSSIVIILSNTNFSKFNTIVDLGGSQGIFSLEILKNNPSIGKAINFDIPLTIQSNKSLLESRLKSYESELSRFEEIQGSFFDANTIPEADCYIGMMNKLLKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0P6</accession>
    <name>A0A8J4Q0P6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000056">
        <property type="protein sequence ID" value="KAF2076567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0P6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="209" mass="25022" checksum="579EA29A8D9B7327" modified="2022-05-25" version="1">MQQSQDVRMNPNYDDEEPEEDVSDFEDVIKQNDVRSLINFFKCDEYVQLPIRIILPAIIENKKRILKVLFKNRSFKHMAHKIHFPLLVHHIIATKDRGLFILVMNHFQPKLYKFLVANRTIVDPYVFYKALDAKDVNFLMACFHRWGQSESVRKTTLKLYKRFLPTLESVGVVIEKIQYIFRDENFFVVFYPDKPFEAYLITPKPETWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q132</accession>
    <name>A0A8J4Q132_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SF4 helicase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_002785</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000081">
        <property type="protein sequence ID" value="KAF2075894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q132"/>
    <dbReference type="OrthoDB" id="275278at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006264">
        <property type="term" value="P:mitochondrial DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01029">
        <property type="entry name" value="TOPRIM_primases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01122">
        <property type="entry name" value="Twinkle_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.580.10">
        <property type="entry name" value="Zinc finger, CHC2-type domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007694">
        <property type="entry name" value="DNA_helicase_DnaB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036977">
        <property type="entry name" value="DNA_primase_Znf_CHC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034154">
        <property type="entry name" value="TOPRIM_DnaG/twinkle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027032">
        <property type="entry name" value="Twinkle-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12873">
        <property type="entry name" value="T7-LIKE MITOCHONDRIAL DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12873:SF0">
        <property type="entry name" value="TWINKLE MTDNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13481">
        <property type="entry name" value="AAA_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13155">
        <property type="entry name" value="Toprim_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56731">
        <property type="entry name" value="DNA primase core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51199">
        <property type="entry name" value="SF4_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="1" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2" id="KW-0639">Primosome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Toprim" evidence="8">
        <location>
            <begin position="337"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="SF4 helicase" evidence="9">
        <location>
            <begin position="467"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="31"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2075894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="716" mass="81257" checksum="85FF3031D5D14DEE" modified="2022-05-25" version="1">MISSQLKTLGCRSYSSTTLGSKFKITLKKPMKPSFNNKFTTPKPDLKQTTTKQDDKDDKDIHSNSNNSNNNKPQQQQNFNKNTSTTYSKPFTQTYSSNNINNTVKSHQKSNTFVSSHFKMNEDSILSYLDRKKLIYKILGQEILIKECPFCSDTKGKLDNLWKLYISRETAAYFCHRCGNKGSWIDFKVNLGDLSLDSSIMKSNGTSGDRNTNAMDDLHHDVNPTDLKKMASYAGELDKHPDVIQKLTGRGPGERGLRLETVQHYNVGVTSQSFFFNDEWQEHKCITFPWTSLDKKGNVVTHRCKLRSTKEKSLQRIEPKGGKWGFFGWHTVPADAKEIILTEGEYDAMAVYQETKIPTVSLPNGANSLPVNLLPLLERFEKIYLWMDDDVPGQEGAAKFSEKLGIQRTYIVQTRQNEINGPKDANDALLAGKNLKQILESSTNIPHDQICDFGDVRSIIHSELRDPLSVVGKQSQWFPTFNKTLKGHRKGELTVFSGPTGIGKTSILSQLSLDFATQGVRTLWGSFELKVPRLAKKMLSQYSNKNLEKNVQDYQQIADQFANIPMYFLRFFGSTHIDKVLDAMEYAVYVQDVEHIVLDNLQFMLSGQGKGIEKFDNMDESIEKLRKFATMKNVHITIVIHPRKQEADSTLSINDIFGTAKATQEADNVVIIQNGKNYRYLDIKKNRFSGDVGTIPLKFNSDTNRFSELDPSELQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1F3</accession>
    <name>A0A8J4Q1F3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Malic enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_001179</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="7">Divalent metal cations. Prefers magnesium or manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the malic enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000027">
        <property type="protein sequence ID" value="KAF2077480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1F3"/>
    <dbReference type="OrthoDB" id="5365701at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004471">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05312">
        <property type="entry name" value="NAD_bind_1_malic_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000182">
        <property type="entry name" value="NAD-dependent malic enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="entry name" value="Malic enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001891">
        <property type="entry name" value="Malic_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010052">
        <property type="entry name" value="PRK13529.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406">
        <property type="entry name" value="MALIC ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406:SF32">
        <property type="entry name" value="NADP-DEPENDENT MALIC ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000106">
        <property type="entry name" value="ME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00072">
        <property type="entry name" value="MALOXRDTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malic enzyme N-terminal" evidence="9">
        <location>
            <begin position="68"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Malic enzyme NAD-binding" evidence="8">
        <location>
            <begin position="258"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="399"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="443"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2077480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="540" mass="60175" checksum="3FF6117217C9F252" modified="2022-05-25" version="1">MDKPSFILRTPATNKGTGFDCEERKKYGLRGLLPPKVENLEEQAERALTQFKSFASNLERYIFLNCLRDRNETLFYFLLGNNLELMMPIVYTPTVGEACQKFGNEFRFAQGMYFATQDKGQMREMMNNWPNQDVDIIVVSDGSRILGLGDLGTNGMGIPVGKLQLYVAGAGFNPSRTLPVIIDSGTNTKKYLEDKFYLGERHPRIPDAEYYPLVDEFLQAAFDKWPNVLVQFEDFSNDHCFKLLDDYRHKYLCFNDDIQGTGAVILSGCINAFRAVQIPLKDQRLVFLGAGSAGIGVADCIMSLFAEAGIPEEEARKCFWFVDSKGLITTTRGDTLTPQKKAYARSDYNHQLKSLIEVVRDVKPTAIIGLSGIGGSFSEEVVREMAKHVQKPIVFALSNPTTNAECTAEQAYSWTDGRCIFASGSPFAPVEYNGHTFVPGQGNNMYIFPGLGLGSVLCKAKHVTDEMIITAAKTLASFVEDSEVVTGKIYPGLNRIREISTKIAINVIEKAYEEGMATLPRPESIEALVKAYQYNPSYNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1Q6</accession>
    <name>A0A8J4Q1Q6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002190</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000058">
        <property type="protein sequence ID" value="KAF2076512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1Q6"/>
    <dbReference type="OrthoDB" id="6019893at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051942">
        <property type="entry name" value="DENN_domain_containing_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005113">
        <property type="entry name" value="uDENN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15288">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15288:SF7">
        <property type="entry name" value="UDENN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03456">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00800">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="2">
        <location>
            <begin position="489"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="375"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="444"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="571"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="445"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="571"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="597"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="955" mass="106763" checksum="10B37B60BD7522CF" modified="2022-05-25" version="1">MIESFQDDEIINTEKRILPSLAEKDKDDKNVVVQVFNETNSNSHNIITSPPLDTPIPTTPPPIIAIENINNNNSYQDSSSSSSCSSSNIPTIQIIQSSPTITTTVVTTTATTTTTEEQNKTIEEYSSSISTMDNISNDNISEINGCSNVEEKSFQFSSNFRSYREGNLIEHFAVVGLPVNTIVNQGDKVSYDGKVLYSYPPDKPCGELHSQIVNLCFPDGIKLIPVKRRNSLTELNSVLFKSLSQLEGSDSSFMFVITGGEHLIYAACVLNTELINELPSFFPESLEGNDGAQTIPPPPYCFFTPRCYVVLSKFPFFNAHFDFIYSIIGKERLNRVNKLYEQQQEFQMKTSLQEPLPSKIPQLSASTSVTDLGKFKAANNNPQKDQSKLTRPNKPLPPLPTQSNILSKSTYPAPSGDSPIQSGVTPTIESPIMEQEETSFLIKQNNNNNNNNNNNNNNINNNSLNNSGNNKLHTRVINHSRSHSTPLSNHLKDIRALAKISKESVPSPTYSTPPTLSDESLSAIQESLGSSTSELSEDSFSDFNSSTNSFSIYHSYNHSLNNSTASINNINNNNLNNSSNQTPIPVSPKPLPKLPDGSLNNGSLSSSHNSIKTSSSNINNNNDNNNNKTFKLPTLLNEELETIEYYFNTQVPLENEKLVLRFPGDQNMTTYESGDDLYQMERWCLGGLMRSMSLKTILQIVSCVLLEKYVLVTSKNIGAISNTIFAILPLLRPYTYQGAYIPILPYATLDALHCPIPYIIGITKIPTDMNDALANCYIVDIDNDKIIIGSNCSPIIPLPNEDKLEAILKHYHQEVHQDKMKKHSIDYPFKINSNDYKFIHAFSDEFRMYHRYIIETIAFYISWDRNFDFGNTSQKYQLLNLVSPIHQVFINQFLNSQLFTSHAELLFEFNLNSSTIINHSGSINNNVNNNSSNGITNVNLSPKDIVLNHYKVNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1X9</accession>
    <name>A0A8J4Q1X9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001481</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000035">
        <property type="protein sequence ID" value="KAF2077227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1X9"/>
    <dbReference type="OrthoDB" id="20960at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14686">
        <property type="entry name" value="bZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="3">
        <location>
            <begin position="434"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="69"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="180"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="501"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="696"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="459"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="769"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="99"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="180"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="330"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="391"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="696"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="718"/>
            <end position="756"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="841" mass="94402" checksum="7FCB7341FC452EBC" modified="2022-05-25" version="1">MYQQQSHYVSAPQQHIYGVAPQQLQQTYQPAQYDYSYTNALNQPQQQQQQQYQQPQQIQHSNNGLIDHQQQIQQQMPHHQQQQQQQQYVDPSSDYYKSGSPSPANYNSMLMATNNVVNNIRQYQPQQEKQVVFGNNMYGGGGAGAGGGGGDNTSFGYLPFSDEKNLILLSALGEGFYCNPQQPQQQQQQPQQQQIQHQHQHQQQHQQQQQQQQQQQQIQHHHQQQQPQQQQQQFYSQPPMTPMSSLSSIPSSPSIPPQQLAGFNSSPAVVSTPPPQVNPFKGMISTPLTPNGHYPNLPVHSSPAIDPQQHKEPGSPFAVITTAGLPPQAPQVQQPIAQAQQVQIPTSQPTPQPQSVQTQAQAPPQTQIPVQTSQQQEPASSSQSTPAGGKTVSTQHCHPQTSLSQYQPSIGKSSAISSYILPQDFEETLANLSEKQKSRRRASQNLASRNYRQRKKAYIGEVETRLDGIVSENDRLKKELFEAKKVIDKLMTENSILKNGGKLPEKSDIPGCTGSEDEDEADFENSLNPIEDELDINVLVDKLEVGMKISTANDDLSSTLKTFYKALKSRQDIYVNQIKQIVNPCTQAKLAILDGEVSGAMRGNPSTLMGGDSCGLQLRGAIQVDSDPPMKDSSPVLSPVSTPIQDLEPTNWWISFCQEASINDEQETRVRQLRQESNYKHKRITKDRKQLNKEIRDYYHSKVFKKEKSNRSSMARENNTNSSSGNNNNNNSNNNSNGNNNNNSNSNPSSDDNMPNIEQTTSPVSTSYLSLLSELLDQLKENLDHENELLLQTYEKLGYILTPYQEALLITKIYNNIFGDEEYSNVQMLHAIWDTILYSYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q281</accession>
    <name>A0A8J4Q281_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">THH1/TOM1/TOM3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000002">
        <property type="protein sequence ID" value="KAF2078593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q281"/>
    <dbReference type="OrthoDB" id="22281at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="328" mass="37993" checksum="4165000466FDBB4A" modified="2022-05-25" version="1">MVMSNNEILAQSIMYFITLVLAIIKTVYLLICKKHSNAFLKEGIHISFVFFLLAKTYTFIGSYAANMTPIPDKYVYVFDLTQVITDHISKIGIVSAYSLLIVYWIGIYEDVFEIRKGKIFTKRTKPLFISLISIFLALEFLVILGKFKLFHNKFRLEIERVELYYTKAMLFILCIIFFAIKGYLFYGQKALSKMTKIKLFSSGILVTCSIFIYTIFTFMIVPGPNGEQFFFWLTCIYFNWLVLYTLFVVYGRDLRTFYEKIFNYFGFENKESSKAMDSVPEPAISNSINVQHDEENGVTPYGESAYTDSTFSDSINTTITTNSNIIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2L4</accession>
    <name>A0A8J4Q2L4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_001080</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000024">
        <property type="protein sequence ID" value="KAF2077617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2L4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="5">
        <location>
            <begin position="126"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="128"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2077617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="179" mass="21300" checksum="1223A7D7A9F1752F" modified="2022-05-25" version="1">MNNNIELNFSFDDFIKEENMLDISDAQQLKKFKEELAKIKESYIKEMMLIDQGCQESINEILSLLPIGNYPIQMKTFLSTHIYYIENLTLKAKLAQRSRCFDKVHHVHTSIVVENKSKKRKEDVFLNQWYDESSANKYPDQQQKDILASRCKISKNKLEEWFGNKRTRESKKKKVQKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2U7</accession>
    <name>A0A8J4Q2U7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation factor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_001713</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000042">
        <property type="protein sequence ID" value="KAF2077004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2U7"/>
    <dbReference type="OrthoDB" id="364892at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01681">
        <property type="entry name" value="aeEF2_snRNP_like_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04096">
        <property type="entry name" value="eEF2_snRNP_like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16261">
        <property type="entry name" value="EF2_snRNP_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000010">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000006">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000003">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000002">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF10">
        <property type="entry name" value="EUKARYOTIC TRANSLATION ELONGATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation elongation factor EFG/EF2" evidence="7">
        <location>
            <begin position="221"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Elongation factor EFG" evidence="6">
        <location>
            <begin position="339"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2077004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="456" mass="50158" checksum="3944060D79A26DA5" modified="2022-05-25" version="1">MVPTSDKGRFYAFGRVFSGVVRTGQKVRIMGVNYIPGKKEDLFLKSIQRTVLMMGRDTKQIEDCPCGNIIGLVGVDQFLVKSGTITTSEVAHNIRVMKFSVSPVVRVAVQPKNPSELPKLVEGLKRLAKSDPCVLCYTEESGEHIVAGAGELHLEICLKDLAEDHAGIEINTSDPVVSFCESVSAVSSITCLSKSPNKHNRLYLRAEPMSMELQNEIEGGTVNAKDDVRSRANYLTDAYGWDSNDGKNIWSFGPDSVGANILVNATKGVQYMNEIKDSFVSAFQWASKEGVLCDENMRGIRFNILDVTLVTDSIHRGSGQIIPAARRVLYAAELTASPILLEPMYLVDITAPENALSGIYSVLNRRRGHVIAEERRVGTPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCIFDHWSSIGVVGVDKRASEVCTNIRKRKGLKEQVPPLENFNDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3D3</accession>
    <name>A0A8J4Q3D3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000580</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000011">
        <property type="protein sequence ID" value="KAF2078109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10492359at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1460"/>
            <end position="1480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1560"/>
            <end position="1632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1568"/>
            <end position="1579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1597"/>
            <end position="1618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1658" mass="186673" checksum="10A5B0EF1592303A" modified="2022-05-25" version="1">MIKVLVFVFVFILFNNVVIIDSLSFNKTLVMDQGCFQSSVSPNSGYMIPDRSKFELTVTLNTNISTFWFHNVDYQSISDQELLLYLNTPYPIFYLYNKETGLDHLPSIAASKGTTYKITLTFTNLPISKNLVFIQDLGLLFNQHSLGPKATISYSTNNMVPPQPTIHPNSLDNCPIIKNIDKSWSIEQPSNMQIYPTIGVNVLECTSSTTNICSSNEKYYYDISFPLSLAKGNIQALVPSFLYQAYRYEFKETKITVGAQSDSQIHLPLEFDRYYSMVSETMMLSKNMSPVESVIKSNPIFISESQVNKVIQDYRSNVYDKVYFTNTNLGFTDQSQQFIIDANTNYQHIINNYQDVYLVFVKNNVGKIYLGVGDKGIARFYLNIVDCPNQSKRNVYTVDAPSSTKHIFLALSVYKGYMLSSLPFIIRNFDVSISNTMMSFHLNHTGTENSVVQQKDSVAISLEFNTQASQYATMVSNSKYLYDININNNREKMESSILLPNSWFGTAFDKIGVELDDLVGQSDFCLKEFGTNIRNQIQHALSDGGYSLDRVLPYYGLVSGLNRIELKSNNVIEMKSKSDYAKVRITTSKPQLKEPVIHAADVEFTLLPGGAGSPCDGRVQVDIRNSGDTKGLVNFQLDCQHDVFIEMINYVAEIGPSDKGSFFTKVFISKYYPTPIQCKVSMWIIGLPLWSDVGKALEKNITFSPYTGCNIPDPCLGVDSEPVLSPTSSSSISSPVSQGHFLNSYTLTTLVQNIGDSNADFQVGVTCLGQQVLYSVLKQNIEKKVNGKQGVEFVHTLYIESLAVDGVLLTCDVDIHLYKPNACWQQQDLQKKQYQLVIPVPYDLCVINSQQQAYIQYSLFNYSHGYANAFFSFNSYSWSISSDQTSYTSIVHHPLQITPNKANQEYIKSNMTAMNIIYRSNLLLQVNNGGNVQGEFTITIDQCSSHLLLLTPTTQTGIINGGAKSLYAVSLVGYATNDEYHYDFCKLKIAITNNTCFDNVGKYHEQDIYVYPHYDACAGNWLEPSLYVSTKEPPPSTANIDIIQDEWRPINKSVFYKDVSFPVRNADYGKGTALSNLVCSGMNTVVVKTSEKSSCFLEPLSSCYHTFRILSDASASQEPLESISCTLDIWFDRSVDTCWSDIGKHVTQVLQITKTVDPCLSFKEPFISTNPIQQLSLNQWAMIEDLVTTKMDYWNTSSPRPVYYKAVKTIQVLNTGDTNATVDSIISILSPTVKLVLDESIPRCIIQAHSYCDLKLTFTTRDVNQELLVNMSRVELLVVDPMICWSGLDKHKEITIQSHLPGPPCAHTLDTPQPWLLYQAEYVGLNQTYFLESDNPLNIDVSPWRDGRVNVSGKVINRGTSGAIIIFDIVCKSNIASKTANTLLVTFIDADKYGYFNLELMLNADQLSLSSSPIIPCVVSATINKQYCWSTFGKSIYQKIPIFPPSLFLSDSSKDKTRDILLGILIPLSLLLIGLLLFYFRKLIPFNLLKLKKKEKKVPMITPQVDSLDPKNEIESFENFEPAEIKESVVVKDVVEEQVKEIPETPVQDMQPETNTERFNRELEQRENNNNIISRRNSIGEIQHQSNPDTLPLIIKNNNEINNDNNNNNSGNEENSNNTRRRRSSLGNITRSRGFTNVGNIIVYVDARSPDMAGKRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3K9</accession>
    <name>A0A8J4Q3K9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_005236</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000203">
        <property type="protein sequence ID" value="KAF2073451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6375767at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051014">
        <property type="term" value="P:actin filament severing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008154">
        <property type="term" value="P:actin polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11293">
        <property type="entry name" value="gelsolin_S4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.950.10">
        <property type="entry name" value="Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007122">
        <property type="entry name" value="Villin/Gelsolin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003128">
        <property type="entry name" value="Villin_headpiece"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036886">
        <property type="entry name" value="Villin_headpiece_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977:SF51">
        <property type="entry name" value="PROTEIN FLIGHTLESS-1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977">
        <property type="entry name" value="VILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02209">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00597">
        <property type="entry name" value="GELSOLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00262">
        <property type="entry name" value="GEL"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00153">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47050">
        <property type="entry name" value="VHP, Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51089">
        <property type="entry name" value="HP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HP" evidence="3">
        <location>
            <begin position="1264"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="160"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="232"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="400"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="673"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="24"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="165"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="184"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="400"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="454"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="675"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="702"/>
            <end position="724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2073451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1326" mass="145527" checksum="DEE9A38941501659" modified="2022-05-25" version="1">MNGSTKTSSSTTTTGKSRIPVRKTTPTSTTLTTKSSGISPKSTTTTTTITTSKIPSRTSLTSASSLSKNTTTTTSSTSSSSSSSSTRVTKATTITSTKTITTSTSSKQQQQQIEENIVDKLQSVNITTEEDEVHESYSVSSKSIQDTSAIEAESTTTLVLEENQSFPPSIATSSSSESGLKKPVSTTTISKIPTGSGIKKPVSSTTTTATKTSTIAQRSTLATSKISTTSSAKSSSIVSSSSSSSSSTTTATATATKSKAIGTSASTKVSTLSIKKPTTTILTAEKKVDVSPAIKKPTTTTTTPSKPTTTAAIKKPVATTTTIKKPTAATTIKKPASATTIKKPAASAATKPAATTIKKPAATTTIKKPATTTTIKKPAATSTLKKPATAATSTIKKPATLAAKKPAPTVIKTTTSSTAAASKVSLATPTKATPVSTPIKTISTTTKSILGSPMENQQMLSSVKGSRPAGPANRKKPTVNPVRNLAGKQSTTTNSSKLSSALIKSNYVTGFGLLASVNNGTEAADQLKKLNHVNSSGNSSSSSSSLRLIKFNGKKSIVGRLVEVSSHSIKSNCCFVLDAGQKIYEWRGQKSSKVQQAMALDLSGRIRNKERGSRSQSIVIEDGKHSPSEQEFWELLSVKSRPKDIVEESQEEEKKSKIKDILYCLINNSNGQQVTTTTTTTSTTTTSSNKTPIKTPVKRSITTPSSLKKPTTPSTPQQQQPLSTFPLKADQIKNPNGKNGLIKEMLKSSNSYILDSVTEIYLWVGKETEDFIRKQSMAKAQEIQAIRKNRPAWVTIQRIVEGAETELFKEKFMDWSKSLPISMAPIPKGRVAETKKEEFKVESIKLNQTLPHSFTTVIDDYKGVVQVWRVRNHNMEPIPNHLYGHFFDGESYVIIYKYIHRNKEVNLTYFWQGKKSTINEKGESARLAVDMDDILQLGVTKKIRVVQNKEPIHFLNIFGGFIIIHSGVLELEKVKDVGILSLKPRAMYHIRSCNHFDSNSNWRVIELDNVSSKNLNTNDWFIIKTNQDRYYVWKGLNSNTSNDNRLESIVNKLIVSKDNNSNSNDGESIKQESIESLDHTLIICNENEEPNSFWKYIDQQDGAIGNIDYFRDIQLLQPIMYQCSLSSGTFTVDRVFEWDQDDLDDEDVMIFDCHTMVYLWIGRRSTQDERKVSMTVVLDLIKKINVESRLKLIKDKQAQQQQMDGADDIVNDIDINLEDYVYLVDSGLEPLEFTKYFHDSWDTRKSRGINQSATTLIKVAEYLKALNRTYTLQELRNNPPRALDSTKLENYLSDQDFESIFKMDKSTFSQQKLWNQEKLKKSVGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3T1</accession>
    <name>A0A8J4Q3T1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000437</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000008">
        <property type="protein sequence ID" value="KAF2078247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3T1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39532">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2078247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="541" mass="63737" checksum="ED56E4B4D6BCA529" modified="2022-05-25" version="1">MSANQIIYDEAINVFNLINSGFEGYYSNKNTTNTTITPIENDESSSLTFSESKRLEAITRFRSFYESKMLHMINMKEYKKFFCLVLLEPSTFVLKQSIQDGALLARISGYRTPFEHERLTKQTLEFIFQDTISIFNQEELNIIQHRLDCFYSVYVNLNSYVYPININNINQLEIPTIPIEIIQKYHKYLASQERVESSLSLSKITRDVQKPSTTSIQQILNNKLLVLKIFNHLIEKNNDQQDQEQQQEEEKRYEFVLLEKQDLNNILNLMLLSRNCFEWCKSILNDKESTRLFYFKPNLRVPYGTEFNLFKQPCQVLNYQKVSSIPYKDFKDLFENLRYLVLRSEDFLIGGVDVDEWSTLIAKQSYLLYPSKTPNIEYLEIHEYCSQISSYQHLIQRIISNGEFTQWFNYTTIENVILPNPGTLVNSIKHLYMNICNEEDSNDTDFHRYLIDLLLLYHPSSIESITIDMGVFCQSFHHIYKLYIDLKKSHPTIKLNIHVAKQIPTSVIMEKITTNSRVPVTQSIQEFINQLKEPNSKITFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3U8</accession>
    <name>A0A8J4Q3U8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ribonuclease Z</fullName>
            <ecNumber evidence="4">3.1.26.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_001013</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.</text>
            <dbReference type="EC" id="3.1.26.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase Z family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.11" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000022">
        <property type="protein sequence ID" value="KAF2077697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3U8"/>
    <dbReference type="OrthoDB" id="527344at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042781">
        <property type="term" value="F:3'-tRNA processing endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990180">
        <property type="term" value="P:mitochondrial tRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07718">
        <property type="entry name" value="RNaseZ_ELAC1_ELAC2-C-term-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047151">
        <property type="entry name" value="RNZ2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553:SF54">
        <property type="entry name" value="RIBONUCLEASE Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23023">
        <property type="entry name" value="Anti-Pycsar_Apyc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2077697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="896" mass="101320" checksum="51DB46A7A8BFFCB7" modified="2022-05-25" version="1">MKAGIKIMANGGGYTDPSIMIWTPDRKYLFNCGNGLHRPNLSSQSKPYEANTLFLTHLDPETTADLPDYILRNKEDVAIVGPRGLTHSVLSHRYYIGRRWKTMYLTECTNDEQYHLCDGYLSVFPIIIEPTLPARTNGGSSKKLRSSSSSLEKEDLNARNKIPMIEKTSIPLNKIGTLKFTEALDPKNYFEWVLLKGGVLHSDTGNKEISMRDIEQYIVYKSLKLDSPIQKESVRQFKPANIKQIKRFLYQKFSDADELDIAPDKDDQNPFWIMESRSFRLVSRLNHSLRFSKSLKRKMLKERTELFISDQHFGPNLSQAKTFPEPPRANNPKSEICSYICHLPDIIGKFDANKANELGIAGPLRRDLCLGKSVVSPKTNTVIHPNDVLSPSTIGPAILVLACPAVEYLANIVSNPTILSYQTSERSGCIFHMISEALFNKPEYIEFVNGFNSDKWQHVILNESSAYHELVPQSARLFNGLNKVSPMFFPSLAPFSAGAPVPETVTVKNIIRGRFQQQISLNPLMPKTQAPVINDQQCTESVDLDFTFDQKEKQDIVVPNVQFAKEQKEMIEQLTDREMEVVFLGTVCSQTSSHRNESCIYINMFDKGGMLFDVGGGSYSQFYRKYGKEGSERALASLKLIYLSHMHSDHHQGLSKIIQARHKALDNLCIPQDQRAMAVLCPLPGQHYIRDMERSLSLYEQPWNYITFYDSNVHNPQLTNFLSASLAIESYKNIPVIHSFASTGIVIKSKSGWILSYSGDTSYCAEFVKAAKGSTILIHEATFKDAQRDKATSKRHSTFSDALHAASNTNSFMTIFTHFSQRYPKIDADVSSPHTNHHCSSPTTKHEDVQHRNAISMAFDFMSVNIKDLVHLPNSLISIKSQTDDDEDEDIDFDNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q430</accession>
    <name>A0A8J4Q430_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000920</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000019">
        <property type="protein sequence ID" value="KAF2077799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q430"/>
    <dbReference type="OrthoDB" id="15392at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="493" mass="55723" checksum="924D94B708DCBB95" modified="2022-05-25" version="1">MVEKSYQAEKSTTGPTIIGNSGSYKDTYDFQVKQQASHLTFDQSPMYLEIQEMAKNVNVDRQQPTINIGRGSDLIQVRNEPVHVNVAQNPTDINVNQPQQVIEVEQSAPEVEIEQYMPKFYLKMPPFRVHLEMDGAPEIIMEPYQPGIKVRQADPIVRVTKPDPRIEVSQHPPKLNITQAPSQVDVTPSTKASIQVKEDRPKISFTEGAPSLKFQEPKTDLKYQAFEPQVIWENPTPRVQVSQGEPSIRIAQDSPYVGLQNKEPTVTINTRAPKIDVHRHPVSVSVLEPSTTVHFGKPTYGAQVSQPHQWVEVQATKTEQVVKESPQQSRSSFILWKSVLTHLDRIVVQQGGGRNCFTLLDTNNSKLCECFKEDLGVHKFNLNIRSSSGELLFRGESLGRILKLFDSSNNLFCTIECNRDQVELFDDKGQKCFALLPHAQKWMFEIKNRSHSNIGTLNKSGKSGYQITFPRDLVSGDKMLLLSSIFLVDDVFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q477</accession>
    <name>A0A8J4Q477_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ras-related protein Rab-14</fullName>
            <ecNumber evidence="4">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_005031</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000191">
        <property type="protein sequence ID" value="KAF2073646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q477"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045335">
        <property type="term" value="C:phagocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090382">
        <property type="term" value="P:phagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04122">
        <property type="entry name" value="Rab14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001193">
        <property type="entry name" value="Rab family, other"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030702">
        <property type="entry name" value="Rab14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0636">Prenylation</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="187"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2073646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="210" mass="23513" checksum="22A64E503B23C902" modified="2022-05-25" version="1">MSFPYEYIFKYIIIGDMGVGKSCLLHQFTENKFVPDSPHTIGVEFGTRIVDVNNKKIKLQIWDTAGQERFRAVTRSYYRGAAGALLVYDITRRITYNHLTTWLTDARNLTNPNTVIMLIGNKKDLEGQRDVTYEEASAFAKQNGLIFVESSAKTGENVEEAFLRTAKLIFQSVQEGNVDLIPDGGITKSNATNTNNTPNLGQEKKSDCAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q490</accession>
    <name>A0A8J4Q490_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_000691</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000014">
        <property type="protein sequence ID" value="KAF2077967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q490"/>
    <dbReference type="OrthoDB" id="73348at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1050.10">
        <property type="entry name" value="At2g23090-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045230">
        <property type="entry name" value="MBS1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026939">
        <property type="entry name" value="ZNF706/At2g23090_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21213">
        <property type="entry name" value="GEO09665P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21213:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN 706"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118359">
        <property type="entry name" value="Expressed protein At2g23090/F21P24.15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2077967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="70" mass="7876" checksum="739D926C69629491" modified="2022-05-25" version="1">MTRGKQKIEDQKRAQEKNAPKKGSQIESRAAAFKLKCHICLAYIINESQVAQHYENKHAGKPVTPLKTDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q493</accession>
    <name>A0A8J4Q493_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Coatomer subunit epsilon</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_000797</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">COPI-coated vesicle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the COPE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000016">
        <property type="protein sequence ID" value="KAF2077919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q493"/>
    <dbReference type="OrthoDB" id="310217at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006822">
        <property type="entry name" value="Coatomer_esu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10805">
        <property type="entry name" value="COATOMER SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10805:SF0">
        <property type="entry name" value="COATOMER SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04733">
        <property type="entry name" value="Coatomer_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016478">
        <property type="entry name" value="Coatomer_esu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 11" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR016478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2077919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="300" mass="34114" checksum="A28DAFAD0A8C9886" modified="2022-05-25" version="1">MSGGGIDILFESKNYFYLGNYQGTINDINKKSKQIADKGLKNEADYYLYRSYIAQGNYDIVLSEIRNSESSPILQGLRLYASYLSSPSQNKDIALLTAKEWIKDGMVQFNYNLQVIVASLFYQEQDYEEALRLLNNCDYIEGLSLLVQIYLKIDRVDLAEKAYNLMKGIDIDATPSILALAWIQIAQGEEKIKSAYACFEELAEKYGPTPLLLNGQAVAAISMRRFDKAETLLLESIEKDSKNPDTIANLISCFINMKKPVEVIDRYLNLLRTISPKHDWMNAVSEAEDAFQRSKSRYGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4N8</accession>
    <name>A0A8J4Q4N8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000001">
        <property type="protein sequence ID" value="KAF2078652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4N8"/>
    <dbReference type="OrthoDB" id="5531344at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2078652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="316" mass="36643" checksum="3D61A75473A5A1A4" modified="2022-05-25" version="1">MEQDDDNVEDENFEIDIDDIGDNTLVGNNSNSHSCTSQSQETYVNKDVCVGNIIELKSTILQYLENINVNNELIKLLNENINLVISKELGDVKEFSSLQPIGTNSKLIKSTQTFLSHTTSLYKEINKKDNINNGFRNKFKRFKKEQQQQQQDFEKQYKSAVVHPENIQVNASKKDLENRIRSFNRLKKHENTENNVIEFRDEVDNENNYSSRTISIPESKIQKSTTFNSTGPLDQFPSDPYATANQNQTSYINERLDILENHLKITVPRVPKDIYQRVKSLEDKLMIIEDSNPLYFKRLYDEANQKNTSNITIPHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q8N7</accession>
    <name>A0A8J4Q8N7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001823</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000046">
        <property type="protein sequence ID" value="KAF2076856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q8N7"/>
    <dbReference type="OrthoDB" id="20709at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040291">
        <property type="entry name" value="DDB_G0287341-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35202:SF5">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35202">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="207" mass="23484" checksum="25AC30627B1930A5" modified="2022-05-25" version="1">MIKFKIIYPILLSIFFITWILLIVSYASYWYRYENKLSQTTLKFKHDRVHESLPGGGHIKYSWTYQGREEQAILRASVSFAILSWIFTTICLVLVALHLFDILHKIPLLPMRYIAKFLPLIPLACSILALLICLGFNRAVQRDCQNTLGSNSVSCDLSWYHSFVGHGEMDSWHPDVGWVVTVFTSAFLLAASVISIGFASFEAAVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4URF1</accession>
    <name>A0A8J4URF1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">mannose-6-phosphate isomerase</fullName>
            <ecNumber evidence="4">5.3.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_006580</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-mannose 6-phosphate = D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:12356"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="5.3.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the mannose-6-phosphate isomerase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.8" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000310">
        <property type="protein sequence ID" value="KAF2072106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4URF1"/>
    <dbReference type="OrthoDB" id="6605218at2759"/>
    <dbReference type="UniPathway" id="UPA00126">
        <property type="reaction ID" value="UER00423"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004476">
        <property type="term" value="F:mannose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07011">
        <property type="entry name" value="cupin_PMI_type_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.441.10">
        <property type="entry name" value="Phosphomannose Isomerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001250">
        <property type="entry name" value="Man6P_Isoase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016305">
        <property type="entry name" value="Mannose-6-P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018050">
        <property type="entry name" value="Pmannose_isomerase-type1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046457">
        <property type="entry name" value="PMI_typeI_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046458">
        <property type="entry name" value="PMI_typeI_hel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00218">
        <property type="entry name" value="manA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10309">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10309:SF0">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20511">
        <property type="entry name" value="PMI_typeI_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20512">
        <property type="entry name" value="PMI_typeI_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001480">
        <property type="entry name" value="Mannose-6-phosphate_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00714">
        <property type="entry name" value="MAN6PISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00966">
        <property type="entry name" value="PMI_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Phosphomannose isomerase type I catalytic" evidence="10">
        <location>
            <begin position="17"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphomannose isomerase type I helical insertion" evidence="11">
        <location>
            <begin position="182"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="295"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001480-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001480-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF20512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2072106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="434" mass="48377" checksum="A218D7B272EF898C" modified="2022-05-25" version="1">MSSNSNSSSSRNKIELLSCVSQNYSWGKLGHDSVVAQLLEKGTTNNNNKSIDQQQPYAELWMGSHPSGPSKIQQCNTNLIDYIHKVKQEQGISLMGDTVEKQFCNDFPFLFKVLSIRTALSIQSHPDCNLAKVLFKQHPDIYKDPYHKPEIAIAITPFEALCGFRPYDQIKDFIKTVPELSNSLPNNILNDHSDCKDLLKSIVTNLLNAKPELIALNLKTLEQRLTQKQEQEKLDKLVIKLHQQYPGDVGVFFAYLLNYLCLDRGQAIFLAAGEPHAYISGDLVECMAPSDNVVRAGLTPKFKDVNTLTQMLTYTTGCPGIIEPIITDPCSGHLSFQPPVDEFQVDLYDFSRLSPNQNTIAVASNPGPSILLVLQGDNITIENDKLTSNNLTNITQGTVLFVPCDTPLHLINNNNSTNQPVLIYQASVNQRVFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4US07</accession>
    <name>A0A8J4US07_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_005684</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000233">
        <property type="protein sequence ID" value="KAF2073006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4US07"/>
    <dbReference type="OrthoDB" id="189968at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000509">
        <property type="entry name" value="U3 small nucleolar RNA-interacting protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039241">
        <property type="entry name" value="Rrp9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19865">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19865:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="309"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="344"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="426"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="493"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="580"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="36"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="99"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="589"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2073006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="616" mass="68998" checksum="25D55B003673A167" modified="2022-05-25" version="1">MDSFFIKPGTAHSLQSNKGSKHAVDSRVYSKPSSSRGGGRGGRGGRGAMGRGGSGSSRGRGGAMSRGRGGSGPNRGRGGRVNSDGNNKYSKQSKQQDDYYSDNEEGEEEEKEQEDQEQDIDNSDDNDNSNDIEQDDDDSEEPQQEDDDDDSDQDSNSNSKKRKFKLSVLEEEETADEKRIRLAKEYLSQFSKFKDANEIGDAIKEDMDRQSGRYQHSYKKSISELKITKDNITYLKGHNSPVTCVVLSDDQRYAFSASRDVIIKWDLIKKVKVMKLNGFIPSKKNREIRQGLSKQERMDAAKHTFEGKILAMALSFDGKYLVTGGQEKRIKVWNADTFEIIEEFKGHQDIVSALTFRKGTYTLYSGSHDRTVKIWDLSQMAFVDTRYGHQSPITAIDALSRERCVTVSTDQTCRIWKIPEESQLIFRGPKSSVDCVTLLAEDKFVSGSQDGSLQMWNVQKKNAVFTKADAHPAAVDPTSISEQGINNSCLNWITSIATIKNSDILASGACDGKVRLWGILGEKLKEINSVDIKGFVNDMVFASDASFLLVASSQEHKFGRWKRLKEGKNCLIFINLSNNSNDNNSNDNSQDEQDDDENMIQDQDEEDDDEEEQDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4US36</accession>
    <name>A0A8J4US36_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ganglioside induced differentiation associated protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_005567</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GDAP2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000225">
        <property type="protein sequence ID" value="KAF2073126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4US36"/>
    <dbReference type="OrthoDB" id="365077at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02905">
        <property type="entry name" value="Macro_GDAP2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035793">
        <property type="entry name" value="Macro_GDAP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106">
        <property type="entry name" value="GANGLIOSIDE INDUCED DIFFERENTIATION ASSOCIATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106:SF72">
        <property type="entry name" value="GANGLIOSIDE-INDUCED DIFFERENTIATION-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13716">
        <property type="entry name" value="CRAL_TRIO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="4">
        <location>
            <begin position="76"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="CRAL-TRIO" evidence="3">
        <location>
            <begin position="409"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="23"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="30"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2073126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="580" mass="66043" checksum="35EC3F949E8356B1" modified="2022-05-25" version="1">MDPTKVINYNSLTSWDQYEEKSRRKTILRNSNTTTTSSTNSNSSTSNNTTTTTTTATTTSTTTTITNNENGANKSIPPFLMDEKINKKLVIWYGDLWRLNVDCIVYSNNPTLSDRTGVSDVIFKYGGSEMLSDVRKNGFECRYGEAIITSAGRLPCKYVVHTVCPSYNAKYLSAAENALNSCYRSAFHLGMENHSKSIAFACLHTETRSYPTSNGTHIALRTLRRLLEKPFSADMQKVVLVIDNITNMEIYQKLLPLYFPRTRAEELNSLNSLPVDIGDENGEATEEDRRIRIIETPSILFRNPDLDEEEEDQNNHLSNMLTKNYLSKSIEEAESIEDIDRIINPFSNESLSGNGHNGTGGRVTHKNSFIFKKEDPDIAKRKNLKSVAAANDSGERMKAQLTNYIMRSKVEDLSHIAKLNFAHQSLDEQGKSLLVIIGFHLNYNDKQLLDHALVYIIKTIELIIQKGNPFSILYFHSNMSSRSSPDFSWLKHLLEIFNLKYTSYLNDFHIIHPTWLLKATLAVSKAFFGEKIFSKMIYHDNLNQLKKTMTNCNIPKSIFSYEFENNHITDFSFETLNESM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UTQ2</accession>
    <name>A0A8J4UTQ2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tc1-like transposase DDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010576</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001209">
        <property type="protein sequence ID" value="KAF2068098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UTQ2"/>
    <dbReference type="OrthoDB" id="31072at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564:SF1">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="1">
        <location>
            <begin position="134"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="324" mass="37958" checksum="4D9C33CB2E194902" modified="2022-05-25" version="1" fragment="single">YANLSFSTSIGKASVMANIPAHVIKYHRKKLISSNYHCKQHGGDRSSHLTQEERVLLAESILDIFKKEPLLTLDHVKIRLENYGFKVTKSSILKDMNWTYKKVEYKQHLKYTDFNLKRYFAFLLWVNTTNWDKLVFMDEVHFSSRDMRKQRSYGHRSETVSVTTKTSLSVSFSMSCMVRFNGDLYTSSYRSTNDQFNSLQFIKDAIEQQYLCSGDTLILDNATVHLAAETHADLMATLQSHGINIHFLPAYSPELNPIERVFGYVKNFVRLNRTQKECDLTFMVQFIFRRIPEITIKKFFMSCLRPWYAEELDEETKSILKNLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UTS6</accession>
    <name>A0A8J4UTS6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010500</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001155">
        <property type="protein sequence ID" value="KAF2068173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UTS6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="515" mass="60770" checksum="CC15D1EF133BD474" modified="2022-05-25" version="1" fragment="single">MDEGEKRTSSLQVIIRNKYLFHLIIEQVSLQRKGDYYIYRNGSPFTKYDEIELPLLLSPNRSKSLLNEKMKNFHRVLVETKEYSNHNSSAEFTTYYQDWIDFNLNSIERFNKGNDQEINIETILSIPTDKHDKLIFIEEFVLKFGLNRKRLELLDKVYPRDKVYNVTTLDLSDIDFLNSLIDQDRIQLKHYGSFEFNPLFYQHKDTIRRIKGWNDILFVNELIRRCLIDNARKFDQQLFTWALTVSPEINWQLFCAEKGVDPSNEPYAELYGVKIRKTEKPFFFLPSHVTSYFLVRKELFIFMETYYPELLSYHFNDDMLLIKNMEIAQLVFNHPAFSPNTKLYTKDAQVYKFFNSKCSINFLFYQNLLTPSFDLDPLSYSSEDLYDLYEIMVVAGSIDLISLINVCIIKGCTDIIKNHFSQQPVPPLFSCLHLSFQSNRMDIFHYLEKVLDPLMSPKERKIQYTELLNSAIAAHDYEYSVKFFEKLQSTITQDILKACLAAVTQAKDQNKAKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UUT8</accession>
    <name>A0A8J4UUT8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">IPT/TIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_002792</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000081">
        <property type="protein sequence ID" value="KAF2075901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UUT8"/>
    <dbReference type="OrthoDB" id="20718at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053331">
        <property type="entry name" value="EGF-like_comC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24032">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035214989" description="IPT/TIG domain-containing protein" evidence="2">
        <location>
            <begin position="26"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="454"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="3">
        <location>
            <begin position="85"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="516" mass="58413" checksum="A0B384736CDE0A38" modified="2022-05-25" version="1">MIRRNIYLFLVFFLSILITTTPCTATSNINSGNSDSTEIPSKAFKPLSAGSSFDADLQKYVFDIVPDVVKDWVNPDHPILPEIFYPVQGYPYENITIIGKNFGNDSSKIDVYVSKMKCTKLSLQSNGSTIVCQPDVPEFPMQELIFHSQPIYIIVDKVLATFNNYFLFIDSAHCPGSVVSCNDHGLCQYGVCQCYRNLTRGGPLCNNVISSPTNYTTNVTSPSFTYYADKDLYWIITFKSLNIYNRDIEEFRTYKVFDSPEWIVSNPDSDTIVYNLKSKEAEASISFYRVPDEKNLSFGGVSAELNKDTIQISVTLSGLPLEKQDNRFNLAINIKTVSVPSSECDPSEIYFKFGRGNSRNDIRWVSVNRNNTSLYGRFYSNCIADGLPQVCAYDLKEDPVLQSKGEANFYVLLEPWKNNVTFRPDFSIIVDPQVPGFTALICKEHVPKQIITDWLLPTILPVFVLGLSCFLMVFYNLLKQNKKTLKMEDDEPSSSSSLIHASCEEESIKTFEDFHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UV49</accession>
    <name>A0A8J4UV49_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002424</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000067">
        <property type="protein sequence ID" value="KAF2076246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UV49"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="64"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="128"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="64"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="89"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="100"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="357" mass="41288" checksum="EEEBFB0C1E809AF4" modified="2022-05-25" version="1">MINPMATQVQSPLQIQTPMTMNPLQQPMFNVPQQPMSMANIMPNNMQPPIPQQAQSPLQIYNSPQVSISPQHNNNPNLVYTPQPPQPVQSPQQPIQSPQQPIPSPQPQQPIPSPQPQQVGVVQQPQQHQQIQNNIQILQNNLNQVNTRINQFTSLKAQVMNNPQQLATVDSNLNTLQQQKNTLQQQLLLQIQLQQQQQQVPQQNVQSQQIQMGNFNNVYQNYPNYGYRPPQQNIQQTQQNYGSIPSPQQAQVPQQQIQSPVAQQQIYQQQANINPQQQQFQQPNINQQQQQFQQQQQQQIQSQQLYQQLYQQTLNSTQMPMQINQQQLFQQQPQNQQLRTVIPILENPVQKIYQPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVI3</accession>
    <name>A0A8J4UVI3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009799</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000811">
        <property type="protein sequence ID" value="KAF2068878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVI3"/>
    <dbReference type="OrthoDB" id="17088at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050606">
        <property type="entry name" value="Calponin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001997">
        <property type="entry name" value="Calponin/LIMCH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003096">
        <property type="entry name" value="SM22_calponin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385">
        <property type="entry name" value="CALPONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385:SF14">
        <property type="entry name" value="TRANSGELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00889">
        <property type="entry name" value="CALPONIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00888">
        <property type="entry name" value="SM22CALPONIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="1">
        <location>
            <begin position="28"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="172" mass="18786" checksum="8D239EAD59902279" modified="2022-05-25" version="1">MSKNTYDPNYNFGLDADLAAKREATYDKSLEDTTRQWVEGIVGEKVEGDFFEGIRNGIYLCKLGNKIKPGSCKSTVSKMPFVLMENINSFLNFCKSLGVATTDLFMTVDLWELQNKNQVIQTLQAVKRITSGSKPAGRTAPSQPTTSTQFCGNCGNKATPGANFCGSCGNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWB4</accession>
    <name>A0A8J4UWB4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009265</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000666">
        <property type="protein sequence ID" value="KAF2069418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWB4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="102" mass="12025" checksum="910544B9FF40CC5C" modified="2022-05-25" version="1">MYIASRKYKSIPTNCQQYQEQLNSFYAPKLITFSIDRDPTSLEIDFQIEFNREKNNSENNNDNLNQRGSLKEVKIDIYDLLSLKQDDLDNNESKDSIRLVDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UY12</accession>
    <name>A0A8J4UY12_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_007430</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000396">
        <property type="protein sequence ID" value="KAF2071248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UY12"/>
    <dbReference type="OrthoDB" id="73901at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF119">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-0187">Copper transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035144871" description="Copper transport protein" evidence="5">
        <location>
            <begin position="23"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="316"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2071248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="358" mass="39759" checksum="2B3464CB3F3B56F4" modified="2022-05-25" version="1">MKQKIILSIILIISIQIGLSSQQGVDCITTPTDPSCANYVYPASSATSDINGLCKGMPYMTVCSLQKACNESSLSTGICQPFSLLAQSCTEDMGGMSDCKNFKSLCTTSGSVVSSCNSTQFIANLPTTKTINTQVKSICGEMNMEDCQKCPNSNGMGYLQCDIFDVYSLLCMAMPDMSQCSTWQSMCVASSPMAQSELSTTFCQLPVAEQTPLMRMFFHTGILDYVLFKTWIPRTPRQFAGAWFAIFFFAIIFECEKTLRSILEKKWEKERLEANEKDDKNSLIINGTIFKGSYPSFSYRDIVKGFLHGLELTMSYLLMLVAMTFNVALFFSVIAGTIFGYILVGRFRSYKPKDSCCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V005</accession>
    <name>A0A8J4V005_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pre-mRNA-splicing factor SLU7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_003709</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the SLU7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000120">
        <property type="protein sequence ID" value="KAF2074970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V005"/>
    <dbReference type="OrthoDB" id="249612at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030628">
        <property type="term" value="F:pre-mRNA 3'-splice site binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021715">
        <property type="entry name" value="Slu7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039974">
        <property type="entry name" value="Splicing_factor_SLU7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12942:SF2">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR SLU7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12942">
        <property type="entry name" value="STEP II SPLICING FACTOR SLU7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11708">
        <property type="entry name" value="Slu7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="11">
        <location>
            <begin position="105"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="259"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="448"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="548"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="162"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="16"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="448"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="468"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2074970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="568" mass="65873" checksum="AACAE629E594A613" modified="2022-05-25" version="1">MSSTTTTSSSLNSQRKSRDEYKKEKELEEARKAGNAPAELDEDGNEINPHIPQYMRQAPWYVDTNKPSLKHQRAFNKKEEVGKGWYQRGAAAQGFTATKYRKGACTNCGSMTHVAKDCCERPRKVGAKFTNDDIKPDEIIQKLEFDYDSKRDPYNGYDPSSYKEIMELYEKADRERKKKKMEEIIKSHKGEENINAEDLTKELEKQEQEQDVYDAENVAPIQKLDPKSRTTIRNLRIREDTAKYLYNLDLDSAFYEPKSRSMRENPLPNTNPNDTRFAGDNFTRTSGDTNKFRDMQLFAWEAQDKGQNVDISSAPSQAGLLHLEFLKKKEQLKEQSKKLLLSKYGGEEHLLNGSNDGSNNNNDISIPQTENYQEYSASGKLIKGEEIVVKSKYEEDVYLNNHKSVWGSYWENGSWGFQCCKQTIKLSYCTGEQGRKLKDNLIIQNLLKNNHNDNNSSSNSDNNEEKEEEPKSLLEQHLEKSNKSTAKEKEEKKKEKKEKKEKEKRLEKALKEQDEINSKSIEKDERKLKYNSLKNDNYQITDEDMEAYNLKRKRSDDPMANFKDSIDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V090</accession>
    <name>A0A8J4V090_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009765</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000800">
        <property type="protein sequence ID" value="KAF2068912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V090"/>
    <dbReference type="OrthoDB" id="21289at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052292">
        <property type="entry name" value="Glucose_repression_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28051">
        <property type="entry name" value="PROTEIN MTL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28051:SF1">
        <property type="entry name" value="PROTEIN MTL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="319"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="185"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="359"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="413" mass="47139" checksum="0064DB5C46C5BEFD" modified="2022-05-25" version="1">MSQIILHDVTTHKSFIPFIHYVYEGEEIPSSLYVPINNNLNDGDDNLSNSAEQQLKSQKKAQCLAPIEIYLNRNGDVENIIPLRDDTSITQVNVDHENEYGSILHIHGIFPRFEENTLAQDENGQDDQSLHNSTSVFDLSKDTIDLLQEYLDRNKLLKRMLLDAQENALYQPPPFLQDNHHSIDHQQQQQQQQQYHHQNLSNQYIHQQPQQQYSNQQTNNINNNITSTPLPSLQHLQNQHQQTSSLQQYIHHQEYLPTTITNTTTLSKPIAKRAINNIELTSPLTYDNQQQQQPQRMPTPISLENNTFISTFSNIRLSGGGGYNKSPPEESPFSSPTHKMIGVDPFIRAPTPTYLSSQNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNSSSNNNNRPNPSPLLGPIKSPFKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0B9</accession>
    <name>A0A8J4V0B9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_003231</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNase II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000099">
        <property type="protein sequence ID" value="KAF2075450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0B9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004531">
        <property type="term" value="F:deoxyribonuclease II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004947">
        <property type="entry name" value="DNase_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10858">
        <property type="entry name" value="DEOXYRIBONUCLEASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10858:SF23">
        <property type="entry name" value="DEOXYRIBONUCLEASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03265">
        <property type="entry name" value="DNase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035298960" evidence="4">
        <location>
            <begin position="23"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="560"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2075450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="637" mass="71967" checksum="F53DE6CB6C61A233" modified="2022-05-25" version="1">MNKGSIFYLLLVVLVSIDPISSCYSGRVDLADSAKISDYEFVDWFFIIKVHAFTDVYLYIDSTMNRPVFKTGYFLRSIESPLGNTMDDYNNKEDTHFVHYNDQDYMDGRHGGGFSGSHEKGFIGIDLNSVDDGDNVWRGVWIQHSMPRFPSGVTTDDYNYFVPPLRYALSEEVKKMYYHFGFSPVAAFFHTNLVAKINLGSSEIEEKSIADEITNMVSFINTYRLGDDIYYRAALYDGGTRSQHALCVSISHKENFLQLLEYLSILSNNGIGGTASTGFDKFLRKEGKVFHSGQPGWVNRKPGNDGNVYLTNKMVKDMKYTKSDGSEVSLPTNTYKNQIKSKIFNFGKNPSNPNYLTPNNAEGEGLFYMKINSAAEQKNDVWKTMVVCSPGTDPEPCLKRPAAGGGKTTIEKITVATWVQSEGDKWWNGIFLTPRFSVKGIDFGNGANEHQWDGNTSNEHSKIAWRHVQPLLPNTELWNVCVSGGNLIPSREKDIKSSVLVCFKSDPLRGALNSITNGGAEDVKNIQKLWKPRKKVIGQLTTILNVSKQDEVTLTRRKNSVVNYRESSSDEDDAGYKAPTRDSRKRKRESVDFSKRKKTRREIMAEDDQQANIKQGKALLESLFPLQQYNRATSPYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0X1</accession>
    <name>A0A8J4V0X1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000158</name>
        <name evidence="1" type="ORF">CYY_008905</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000596">
        <property type="protein sequence ID" value="KAF2069782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AJWJ01000003">
        <property type="protein sequence ID" value="KAF2078533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0X1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="99" mass="11538" checksum="574E9078FA821A0F" modified="2022-05-25" version="1">MRPHFQVQEIKSLPFDKFSTCVQVNHDICSHDYRLRFTENTIGCLRECVLVCLETTNPSRIQDFVFPDTSVDEMKEVSLPILEGYMKLKNIEPKLANDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1M1</accession>
    <name>A0A8J4V1M1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_008108</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000473">
        <property type="protein sequence ID" value="KAF2070577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1M1"/>
    <dbReference type="OrthoDB" id="8061355at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030587">
        <property type="term" value="P:sorocarp development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000665">
        <property type="entry name" value="ABC transporter A family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056264">
        <property type="entry name" value="R2_ABCA1-4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF154">
        <property type="entry name" value="ABC TRANSPORTER A FAMILY MEMBER 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23321">
        <property type="entry name" value="R1_ABCA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="326"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="361"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="394"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="424"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="468"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="546"/>
            <end position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2070577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="857" mass="97614" checksum="512ADAAF31292A7D" modified="2022-05-25" version="1">MDINNNNSYNKNNNNFVFTDAKGNPISYAQGNKIHNQDELKLKRNFKSQFNAVLKKNVTYQWRQRKNNACLLVNIIFLVVFMCIINLIVKVYLKDYSTPAQLHPYAGLPSLYLTNSDKCIPKMYPESLFYLDHVITNTNATNEIINNIQQSEQPIGLIFGQSLLGKASTFCKVGAQSLIYPLTMEKIDSNVTQLNDEIFHSWRRLSIMGGYDFKSINNENLELTIVFNKTLTRYKDLPTLFQLVTHAYNSLIGSNSNFTSIVYDGLRDVPHGKIEPNFDLVSLMGPMLYMFIFQMPLPFILKLLIYDKETKLKDLMKMMGLEMKTYWISNYIVSFLLYFISIIFLWAIACLLGFKMFLNNAAIVIFILILAFGHLLVSFSFFLSVFIDRYQMGLYFGYLWVLFTGLFTNNVIHDILDSKFTPESSVFAISLVPAFLLYRGLLGLQNGALIEEITLDNWNDRDYLMKDVIVYMVIEATIFLILALYLEEVVPSKYGVKRHPLFIFKKSFWFKKFNEPITLRPILEGEGPDVHAERDRILNNPQIKALEVLDLNKTYGTKQAVKSLALAVDQGICFGFLGPNGAGKSTTISCVSGLFQPTSGTARVLGMDIRNHFDRISMVLGVCPQDNVLWEDLTGEEHLLFYGRLKNINGKRLKEAVDASLKSVYLDTEAHKMVKQYSGGMKRRLSIAISFMGNPKLVCLDEPTAGLDPATRRGIWNSIKEHKGKCAILLTSHAMEEVQELCDRVGIFVEGEMKCIGASHELMSRYGEGYKIKVSIQPDREQYVTHFITSLVPGARITHSMNNIIQYQLPRSDSVKLSVLFREIQLNLDVGIIDYGISGTTLEEVFLKVCNKKYNEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1R7</accession>
    <name>A0A8J4V1R7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010366</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001065">
        <property type="protein sequence ID" value="KAF2068309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1R7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="261" mass="30564" checksum="FD88A4866DF89857" modified="2022-05-25" version="1" fragment="single">IIIHNNNNNNNNNNNNNNNNNNNNNNNNNNNNSIKSSNEEYSISHNSHSPSFCKSSPSKCNVHNHHSSTDHRPHKKYLEESTDFINQSPRRLSSDNHYNFTFKPSQFRNSFDYIPDDNDDDDEIENINFKRDEIEEEEEEEEEKELPQPLQEDTKSTSFKDSFFQNALSQFSKFKIFDEDEPELLELDLPIESNIDDFDSGGDCNSFNNYNSNYNNNYSNSLNNTIGNNTNSNNNNNYRSMNPMANYLEKKEKKWKPVIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2U8</accession>
    <name>A0A8J4V2U8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Regulator of chromosome condensation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006736</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000324">
        <property type="protein sequence ID" value="KAF2071942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2U8"/>
    <dbReference type="OrthoDB" id="19412at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051625">
        <property type="entry name" value="Signaling_Regulatory_Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872">
        <property type="entry name" value="BTK-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="25"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="80"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="140"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="189"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="291"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="413" mass="44537" checksum="1EB8D67D467C1C67" modified="2022-05-25" version="1">MIRFTNQLLYSARVVGTTNKSPLICSLYSWGSAPNGQLGLGRNITEQYTPKLVEFDDGCQVFDTDRLDSGSFHAILAKDKKIYSWGHNRFGQLGHRNTDKQVDGCFTPTAIDFFNGKDIVASACGSYHTVVSVKDGEKGYSTYSFGWADRGQLGHTGSGVIQEIPFLKDKKVVAISCGFDYTVVVTESDGIYTFGANDKGQCGTTSSAVQQPIRVHALDGVKADEVSCGWGHTLLKTTDGELISWGANFHGQCGQGGPLKHHIAPIGKVLLDRPVSSPSSGSCASSCIVDGQVYIWGSSGDGKLGLGETFKDDLAQPTLLTTLPTNTKYTSTSFGYDHCIALSDTNHSIFFGWGFGQHGTLGHGSHTQSDLKVFSSPSVNRYFENINQSISTIHCSLDTSFAIVYPNTTNNTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3G7</accession>
    <name>A0A8J4V3G7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SNF7 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008916</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000598">
        <property type="protein sequence ID" value="KAF2069764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3G7"/>
    <dbReference type="OrthoDB" id="5594417at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10476">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="209" mass="24106" checksum="514CF1E01445F3A3" modified="2022-05-25" version="1">MNLFNNKKDPNKELKESKRELTKSSREMDREINRLRIVEKDYLKQIKSLALANRMDEAKRLTRDLVKLREQIERLQSTKTTMSAVSTKTTTIASQQKMAKAMENATKAMSLANAQYDVAKLQKTMNEYQKQVYHADLSEEMLHDMFEDEEMDQEADDILSKVVDEVVLDNYSKMPSVRDGSLPTAIESSSSKREVSDDEIERMLSSLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3T3</accession>
    <name>A0A8J4V3T3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_005782</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000240">
        <property type="protein sequence ID" value="KAF2072887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3T3"/>
    <dbReference type="OrthoDB" id="16815at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050308">
        <property type="entry name" value="MukB/SMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42963">
        <property type="entry name" value="CHROMOSOME PARTITION PROTEIN MUKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42963:SF1">
        <property type="entry name" value="DUF4476 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="371"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="723"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="555"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="664"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="391"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="729"/>
            <end position="748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2072887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="823" mass="93686" checksum="3B72924A0C647773" modified="2022-05-25" version="1">MFFKGKKKEKEEGGVVHGEQPNGVTTRNIIRIGSVSSGASTEQLDQIAANKQKIPSLFRLLAEHKLSEKEIWEGDPMLFAILYQFKEHLNKTDSAPNIITFHLPSIPEFGDIKKKSYKINKKFSIAQTISLICKKQMIPDPKRFWIGSLQGFIMNDTETLASYGLSSLFESWELRLIVKEDILSKSPYRYQPLESIGTEFIVVFELPPLEQFNGLKRHVVKVDSGHTIKQTIANICKKYKVENPDRFSVMTIDENPLVEFALFGGACFKHYGLGYKFKKWDLKIVFNDLIPPYVHKHTVIPLHGWTQVSPGQLDPYTARTIINDLENKCVEYQAIITQLQKDIEGGNEVSAAHVREIERLREENKKLLETLEEERESTSQQNEVYEKRLESSNAEIESQSGQMKTMKAKIDELSHQLSQLKIVHEETTNNLVVSRAEKDSLQVQFEEATAEIADYQQKLLLLRAQSAQSESNLTSMIATLEKEKIAIKEEAKLSFERIATEKASSEESSKHLQASQALLCTQLQDDIVSLQREREIMQLSVQKATVDRQEAVVLQEKAEKKMVEMQSEKKRVERQFGDLERERDVLRQQVQIKVDEIQDVQKDLEWSRETIAELERRLANMTGEVKMFKDIKDKEVQKAKKSCVSETQTKSQLDKEKQNTLEIQTRLNKKINELESARKAQEQAFKNAEQAFKKVEGDLKRETEDYRQKYEQLRISISNGTIKEGTNVPALPAAASSNPNGSSFSLGGHKASSANKDLNDDMASSPVLIGGVQKSQEDSSAQNSSAHERRGSVDADGLYNSLQNRFQHTVREGVEELELSDNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4H8</accession>
    <name>A0A8J4V4H8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_005132</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000197">
        <property type="protein sequence ID" value="KAF2073547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4H8"/>
    <dbReference type="OrthoDB" id="1919336at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09011">
        <property type="entry name" value="HMG_box_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="3">
        <location>
            <begin position="139"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="1">
        <location>
            <begin position="139"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="75"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="167"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="167"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="208"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="241"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2073547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="357" mass="41360" checksum="12223E1325F53AB9" modified="2022-05-25" version="1">MVKKTSTTTPGTSPSQHNTVLSSKKNAKPGTHKKATTTTITVEKDPLKTVNKSNKATTDDDEEYDLNEAIKSFKIKRKQKKEQHELEIKAHSPSSHTHSHMHANHPTTGVKKSKPQTAKTAFQYFKEANSTKTPGFCLEKWRTMTDVDKSQFYESAQKDKERFNSQMAEWEKLKAQTEHHKVQKKDLKKATSSDEEKKNNGPKKLTKKEKLIKEKEKKEKVKQERINKFAKKKKDEKEGKEEDDEEEEDDEEESESESGDQDEESENDSGDYDEDDEEEDDEEDDDDDEDDDEDDDDEEESDDDEEEDNSDREKELYNEDGFIDEDGGDGWADMKKGFKSSHHNSSQKQARKYKWNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4P5</accession>
    <name>A0A8J4V4P5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007714</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000427">
        <property type="protein sequence ID" value="KAF2070964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4P5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035210500" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="22422" checksum="A23DC91027B4FA5A" modified="2022-05-25" version="1">MKITIFLLLLIFNFSWGNYLSLLVNRNNYWQLVKESFYSYNQNIVYDIEFPSKDTSVLLKFVVQPELVGHSINFTLYSYFNHQLNVAFNSINYQHSSGEGYLFQLCNPTLEIPFNLQLNNPNKGGVITLKAKVDNLVSCQAKNDSSVNDLPESFLKRNKLKEISYEIDYTTGESNILQPLLFLTLFLFLYQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V573</accession>
    <name>A0A8J4V573_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Right handed beta helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004372</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000153">
        <property type="protein sequence ID" value="KAF2074312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V573"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31318">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31318:SF2">
        <property type="entry name" value="PECTIN LYASE-LIKE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035240875" description="Right handed beta helix domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="469"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="549" mass="59491" checksum="293A682B8209A48C" modified="2022-05-25" version="1">MIKPSILLSLFVLLFVVCGGVYSETTVLYVKSSAQQKTYCGAYQSDPCSSIDLALENFKKTTSPAKYSNGVTVVLMDQIYNLVSESVEVTGLNVTITSNQGIQTTIYGQDRSQALFVIKPSIQPTTFIIDRVAFQSFNSGPIANFQFSSNGSTNKVTLKRVIVTESIQDGAVLFNVSQSLKGSDTTFNIFDSLFDHNNVLLMDAYKISVNIQNSSFYNNTNDILFDLHQTNSTINNCLFNFSGSITFDYGGSAFINSSTFSNGTSAYPPITTKSDLVVSNSIFQYNIGNSAGGILFIGSHFKINNCQFINNNGFAGALSFTSSSMGFVQNSLLLDNYGSGSMAFYASSSTVSVSNTNITTSFAMQGSYNQLIDVEYSTISFSNSIVSSQSIPWGTFSVVSCTTSSISVANTTIDSNGYDATARNHGNNILFDCVPSSCLVANIDTSYDNSPTYQCDKFQYRDYDPVVRVYLLTGLIISIGFFVIGVIIIGCCIKRSKACKDNTIQPEGNSFVINIVGETKPLVANNNNMNNKIITPTAIITAPSGYSYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V585</accession>
    <name>A0A8J4V585_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_004348</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the DIM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000152">
        <property type="protein sequence ID" value="KAF2074327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V585"/>
    <dbReference type="OrthoDB" id="147752at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02954">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000004">
        <property type="entry name" value="Spliceosomal protein DIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004123">
        <property type="entry name" value="Dim1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12052:SF5">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12052">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEN 4A, 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02966">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017199">
        <property type="entry name" value="mRNA_splic_U5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01410">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4 6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="38"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR017199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR017199-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2074327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="142" mass="16488" checksum="9D4C99E5104A2904" modified="2022-05-25" version="1">MSYLLTHLPNGWAVDQAIVTEEDRVVIIRFGHDYDPDCMRQDETLASIAEKVKNMGVIYVVDITEVPDFNKMYELYDACTTMFFYRNKHIMIDLGTGNNNKINWPLTNKQDMIDIIETVYRGARKGKGLVNSPKDFSTKYMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6M0</accession>
    <name>A0A8J4V6M0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Gamma-glutamylcyclotransferase AIG2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005701</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000234">
        <property type="protein sequence ID" value="KAF2072989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6M0"/>
    <dbReference type="OrthoDB" id="113620at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036568">
        <property type="entry name" value="GGCT-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110857">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2072989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="24277" checksum="D336EA992753F186" modified="2022-05-25" version="1">MNKIKSIFVYGTLRPDDNSSAPWTLSFSKSLVFKKCFFPHGIMFHDEFPVVSLLYKNIHACSANGDKGHHDINLFDEEIDTESDLYKLYNELNCNGIVGYVATIDDSLLTNNNDTDEEEFDRLFKEKLIEADEIEEYPQLYERSIIKVHPIIDDHSTTSHITTTTTTTTTTTHKYDKENHIYCYIYHIQNCNREKIIRSGNWLTRHLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6P0</accession>
    <name>A0A8J4V6P0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_002682</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the cytochrome b5 family. MAPR subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000076">
        <property type="protein sequence ID" value="KAF2076022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6P0"/>
    <dbReference type="OrthoDB" id="547796at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000003">
        <property type="entry name" value="membrane-associated progesterone receptor component 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050577">
        <property type="entry name" value="MAPR/NEUFC/NENF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281:SF72">
        <property type="entry name" value="NEUDESIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="9">
        <location>
            <begin position="48"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="150"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2076022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="174" mass="19829" checksum="22D2C6EAEACB52A8" modified="2022-05-25" version="1">MLDFIPEEVTWAILIFIIIYLLRVILTPKEIVVPPVRPKKPTYEKRDYSLSEIKKYDGNTTGEPIFIAIKNKIYDVSAKSSTYGAGGSYHLFSGNDATVCLAKSSFEQADINQPWSEESIKQLSADQQDTLNNWIQFFSERYPIVGNVKDENSVVDNDDKPKIEEVDEDEKKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6X8</accession>
    <name>A0A8J4V6X8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 15">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="11 15">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">CYY_005352</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11 15">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 11 15">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="11 15"/>
    <dbReference type="EMBL" id="AJWJ01000211">
        <property type="protein sequence ID" value="KAF2073334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6X8"/>
    <dbReference type="OrthoDB" id="361536at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02658">
        <property type="entry name" value="Peptidase_C19B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14385">
        <property type="entry name" value="UBA1_spUBP14_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14386">
        <property type="entry name" value="UBA2_UBP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000086">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000026">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016652">
        <property type="entry name" value="Ubiquitinyl_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041432">
        <property type="entry name" value="UBP13_Znf-UBP_var"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF664">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17807">
        <property type="entry name" value="zf-UBP_var"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016308">
        <property type="entry name" value="UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 11 15" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 11 15" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7 11 15" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10 11 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="19">
        <location>
            <begin position="166"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="18">
        <location>
            <begin position="319"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="17">
        <location>
            <begin position="628"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="17">
        <location>
            <begin position="693"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="670"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="683"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="786"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR016308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR016308-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR016308-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAF2073334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="824" mass="93203" checksum="AB174515E9FC0104" modified="2022-05-25" version="1">MTDDITKLSLNIKVPTNSSRLWKDECCYSFDTPEKDTGLYIDLISLLSFSKKFIPLNHQKTGHNLYLNFRKVELPPSSPKGVSTHSDQQHAPKKLAIGVEGGFDANEEEIKYEDKYQLYIFPVDKYIDINDKEIPHNVKECCEKIMTMNTQSRKEEVMSWNAENVLASAYAESIIQLETTRKINPKGPWNCDIEGCDKKENLWLNLTDGFIGCGRKYADGTGGNGHAQKHYDETKYPISVKLGTITKDHSDVYSYPEDDMVSDPLLTQHLAHFGLDPKAMSKTEKSMAELELDQNLNYEFGKLQEKGRVLENVFGPGLTGIENLGNSCYMSSVLQMLFAIPDFQTRYLFDREKSYKEITQDPTLSFEIQMSKLAYGLLSGDYSVPAPKNATKNKEEFDIASQVGIAPKMYKSLVSSNHPAFSTMQQQDALEYFTYLLEYIERAEVIRPSWIQQSNPLQTFQFHLQERIQCGTSGKVKYTNRVENYISLPVPLEDAINKEQVAQYEAEVEAIKASGEKVPADKEEVRPIIPLTSCIQRFVEPYKVDDFYSSAIKSKTFAINSSKLATLPETLVLHMRKYVFNPDYTPKKLNVCIDVPDFLDIEHVRGFGKQDNEEELPEDNGGGNDEPEFDQAILDFLLSMDFPLVRCKKALVATGGKDAEAAMEWIFKHTDDPDIDVEPTPKPKTNTTSSQPTFSQDSIDSLCSMGFTEKQVKHALKNTSGNVERAGDWLFSHMDELDSITEENVVAPSSSSSSSTTAASKLKDGSGKYKLLGFITHIGNNAQHGHYVCHIVKDGKWIKFNDRHVQLSQDPPKDMGYIYFYQRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V878</accession>
    <name>A0A8J4V878_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003975</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000132">
        <property type="protein sequence ID" value="KAF2074744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V878"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="95" mass="11181" checksum="6247B23A05F50DC1" modified="2022-05-25" version="1">MGIYLRRIIRNYVVENKEIRNSFSYLNANYKELALLDKEKYNILICLVIDHPYGRLMDYGIEDTKMDFVLQVFDSPHIGIVNSLEINQPTEKLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8C2</accession>
    <name>A0A8J4V8C2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_000988</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000022">
        <property type="protein sequence ID" value="KAF2077672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8C2"/>
    <dbReference type="OrthoDB" id="10009520at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16773">
        <property type="entry name" value="RING-HC_RBR_TRIAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14298">
        <property type="entry name" value="UBA2_scUBP14_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000007">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045840">
        <property type="entry name" value="Ariadne"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048962">
        <property type="entry name" value="ARIH1-like_UBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033864">
        <property type="entry name" value="UBA2_scUBP14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19422">
        <property type="entry name" value="Ariadne"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21235">
        <property type="entry name" value="UBA_ARI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBA" evidence="10">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="227"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="46"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="78"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2077672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="578" mass="66238" checksum="E826D020D3AF6A45" modified="2022-05-25" version="1">MDDNMLAQLVDLGFDITVAQLALEQTNGKGLDAAIEFIYSFSEPSKLSSQKSNSNVTPTPTPPSSQQEPSGIRRSNSKNKSTSFEPISPQVTHKVLKTSRKSVKESVDSNVSYEDNDTSLEYNDTDEDYDEDYYQDVDDYEFDIPIHDALPKSNTVIQASDIIHTALKEIEKISSITETTPSAATILLQHFQWNGNKLLERFYEDPERVITQAGIKKINEFHTGKNLSDDCMICGDSLDDSNGCYLSCKHYACKECWANYLRIKIQEGESIGICCLGFKCPNVVPDEFINRVIPENYNKFLERLAQTYVEKNPNMRWCPTPNCGNALKAESQAETTAQCSCGFRICFKCNQESHVPADCAQMKTWKKKCEDDSETANWINVNTQDCPKCQSAIEKNGGCNHMTCKKCKHEFCWVCLGNWIGHNKCNTYNREENSNKSDTKKQLERYLFYFHRYNTHEQSKKFETKLRTDAVNSMIDFQNRKDKRWIDVKYIEQSTEVLIQCRRTLKYTYVFGFYLPDGPEKNLFEYLQNDLERTTEKLSGLLEKMNESNSMDVKEITNLASTKLHHLLSGVEEGFLGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V982</accession>
    <name>A0A8J4V982_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002858</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000084">
        <property type="protein sequence ID" value="KAF2075824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V982"/>
    <dbReference type="OrthoDB" id="9984693at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033308">
        <property type="entry name" value="PGAP5/Cdc1/Ted1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315">
        <property type="entry name" value="METALLO PHOSPHOESTERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315:SF1">
        <property type="entry name" value="PROTEIN TED1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="6">
        <location>
            <begin position="23"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="363" mass="42730" checksum="DA2D383A96517B93" modified="2022-05-25" version="1">MFFCNWKYNINKENDIAQMILLGDPQMEGDWRIKREGLKGEYNIAFNDRFFKHIVDNIAYYLQPSLVFVLGDLFSSQYINDDEFLVRTNRYRNIFSPLLYKQSKSELKIINVTGNHDIGYANEASLHRINRFEQAFGKVNDKFYVSGHLFGVVNSINLDRSQDERLYNQTWDHLRALKEEAESTQSPLIILTHIPLYKDIDTIDRSLPLYQQQPFLCRERDHTRKDRNGNIIEQTMISKESSDYILEHLKPLFIFNGHDHEGCIFKHQNNITTEYTIRSMMGGYAGYSALFELKKLSKSSSPSTKEEFEYSFQWCPFIETRFINISFGVTGGWLVLFILFNLVLSFVVPFINKHKQDKKKKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJC4</accession>
    <name>A0A8J4PJC4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_010203</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000972">
        <property type="protein sequence ID" value="KAF2068470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJC4"/>
    <dbReference type="OrthoDB" id="15219at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051052">
        <property type="entry name" value="Diverse_substrate_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942">
        <property type="entry name" value="METHYLTRANSF_11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942:SF4">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="4">
        <location>
            <begin position="49"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="267" mass="30536" checksum="1E51CA1B1524399E" modified="2022-05-25" version="1">MTTTLHPTAKAYHNDETTNAYVKGRPTVSMESLEYLIQELNLNKNSRVLDLASGTGKFTKLLGEKFNKVEAIEPSPQFREACKNELEIIKNTKNPELEYSVLEGLSTNIPKQDSSFDVVFVSQAFHWFANIESITEISRVLVKGGALVMLWAKIDEQDPISKSIMFQGEKYHDEFTPQYRFGTWQEVFDKMKEKKFEPINLNSIVDPNLKQKSFTFDQETNKELLTCLAMSISFISLLPQEKKDQLIEKIEKILESNPISSNGKILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJG3</accession>
    <name>A0A8J4PJG3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Poly A polymerase head domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_010024</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000889">
        <property type="protein sequence ID" value="KAF2068650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJG3"/>
    <dbReference type="OrthoDB" id="445712at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052929">
        <property type="term" value="F:ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052927">
        <property type="term" value="F:CC tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001680">
        <property type="term" value="P:tRNA 3'-terminal CCA addition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05398">
        <property type="entry name" value="NT_ClassII-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000019">
        <property type="entry name" value="tRNA nucleotidyltransferase cca2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3090.10">
        <property type="entry name" value="cca-adding enzyme, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002646">
        <property type="entry name" value="PolA_pol_head_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13734:SF5">
        <property type="entry name" value="CCA TRNA NUCLEOTIDYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13734">
        <property type="entry name" value="TRNA-NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01743">
        <property type="entry name" value="PolyA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81891">
        <property type="entry name" value="Poly A polymerase C-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly A polymerase head" evidence="6">
        <location>
            <begin position="110"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="36"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2068650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="511" mass="59148" checksum="A288AE518EEE5524" modified="2022-05-25" version="1" fragment="single">MSDSPIEHSIKKLKNAKHFIPITMISNIEKHDSNYFHKLPHHQNNNSSNNDKQHEHQHEQQLQQQQHTTITVGINQDSTETTQIELTESEVNLFKILMDVVKETNCKTTLRVAGGWVRDKLRGDDSHDIDITLDNMMGESFAELVNNYLGNHQQKTHRIGVIQSNPEQSKHLETATVKIFDMWIDFVNLRSETYTEASRIPEITIGTPKDDALRRDLTINSLFYNINENRVEDYTGNGISDLKMGIIRTPLPPLTTFLDDPLRVFRSIRFATRLYFAIDKELMDAASNPLISNAIKSKISHERIAKEFDGMLNGPRPDLSLYLIHHLGLYDCLFSLPPGFNDIMQDYKEKSVYYCYQMMRLINWGTMHEEEQQSTKRIRILSSLMIPFGGKLYSSQAKKNRTYTVVQYLLLDYLKLSNKDNEEVSLVLDCSSQFDTIIKHYIENKSSFNRKNVGLILHKCGPLWRVALLISLLKLLPIENFNHTFPLHNNSDIDSHKPLDYLSHQHPHHLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJQ1</accession>
    <name>A0A8J4PJQ1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_010534</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001177">
        <property type="protein sequence ID" value="KAF2068138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJQ1"/>
    <dbReference type="OrthoDB" id="15822at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08268">
        <property type="entry name" value="MDR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE PIG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106:SF5">
        <property type="entry name" value="ZINC-CONTAINING ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="3">
        <location>
            <begin position="17"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2068138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="339" mass="36883" checksum="9E30563AA2828E57" modified="2022-05-25" version="1">MSLNTKQNKKVIFEELGGVDKLKVISTDENKKPGKGELGIRVKALALNRADLLFISGLYGKLDLPTGLGFEASGVVEQIGEEVDGFKVGDSVSVGPNISIAKYPLSGEYVLVPENSLIKNPDNVSHNDMAANWVAYFTSYYGLQEAKLTKGDTVLINAASSSVSIAAIHLAKYWGARVIAVGRSNKKRSLALEHGADHYISTQDEDLVAKVKEYTDGKGVQVIYDSVGGPQFSLFGEIASIGAKIVVYGALDQAPTPIPMYQLIFKSIQILGFTLGRYLTADHDEKRLQVTQELNQLFSSKKYKSLVGKVYKGIDSYIPAVEFLEKADLEGKIIIDLSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKE0</accession>
    <name>A0A8J4PKE0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000923">
        <property type="protein sequence ID" value="KAF2068572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKE0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="57" mass="6642" checksum="4F37A4A89E2C3E45" modified="2022-05-25" version="1" fragment="single">MDNENSPQSVEGSKKRKYVSPINAFQDLVQEHLESLEQEIKDLEKRRDTLLDVTKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKF8</accession>
    <name>A0A8J4PKF8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000666">
        <property type="protein sequence ID" value="KAF2069417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKF8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="29598" checksum="FCB1579A82B0DE1F" modified="2022-05-25" version="1">MESSIPMISPAKRVYKVEQTFALKWYFTGFNKAYKEELNGLLNKEEYDQIIRRANKIVSRTLIYIASLFYGISAFIFFYMLFANPYSFQRHIGLLCAVILIPSVLILILSMRYFYTVPKQSQAFKQELNALYAHRNLKFSLKKSRKPVISYDLKIKHINPPLVVAQIPQFVQPQFIQQPMQQPQFQPYPYVQDYNIPLTQPQPQYIFTNQIQQPQQQPQFILTTPLPEPQYVQPPSNHQQYIPITQNEHTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKM6</accession>
    <name>A0A8J4PKM6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Translocon-associated protein subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010134</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000939">
        <property type="protein sequence ID" value="KAF2068540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKM6"/>
    <dbReference type="OrthoDB" id="5860827at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12861:SF3">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12861">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN, BETA SUBUNIT PRECURSOR TRAP-BETA SIGNAL SEQUENCE RECEPTOR BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05753">
        <property type="entry name" value="TRAP_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035280041" description="Translocon-associated protein subunit beta" evidence="2">
        <location>
            <begin position="24"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="185" mass="20932" checksum="F4539C72238E3FAA" modified="2022-05-25" version="1">MSYKTVFSLFLILCVSIAYCGKAELLFHKKIVDSPVVGKELPVQFVIYNVGSEPAYELEFDDKDFLTSDFEFVSGSSAGKWESLAPQSHVSQNITIIPKKAGIYTLTSTVLNYLSSQHEKEVSITTAASYSGMYVESLQDYDKRTSLHVKEWTLFVLFSLASVAFPFSIWAYYKVNYEHGIKKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL30</accession>
    <name>A0A8J4PL30_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_009852</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000830">
        <property type="protein sequence ID" value="KAF2068825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL30"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10241">
        <property type="entry name" value="ASKHA_NBD_HSP70_BiP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000153">
        <property type="entry name" value="Endoplasmic reticulum chaperone BiP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000172">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000001">
        <property type="entry name" value="heat shock 70 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000026">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042050">
        <property type="entry name" value="BIP_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035329318" evidence="8">
        <location>
            <begin position="21"/>
            <end position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2068825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="657" mass="72080" checksum="88D38195B9C0EB9F" modified="2022-05-25" version="1">MKNIIVGLLFVLFVSAVVFAEDTNVNDADKVLGKVIGIDLGTTYSCVGVFQNKKVEIIANDQGNRITPSYVAFTESERLIGEAAKNQATLNPENTVFDIKRLIGRKFTDEDVQRDMKLLPYKIINKANKPYVSVKVKGEEKTFSPEEISAMILTKMRDIAQDFLGQTVTHAVITCPAYFNDAQRQATKDAGVIAGLKVLRVINEPTAAALAYGFGDKGNEKNVLVYDLGGGTFDVSVLTIDKGVFEVLSTNGDTHLGGEDFDQRVMKHFLSVFQKKTGKDASKDKKSLQKLRRAAENAKRALSSSHSTPFEIENFFGGEDLMETLTRAKFEELNMDLFKKTLEPVKKVLQDAKLKKSEIDEIVLVGGSTRIPKVKQLLKDFFNGKEPSQGVNPDEAVAYGAAVQGGVLSNEESTGDVVLLDIAPLTLGIETVGGVMTALIPRGTYVPTKKSQVFSTYQDNQDKVLIQIFEGERSMTKDNHLLGKFELSGIPPAARGVPQIEVSFEMDVNGILHVSAEDKGSGSKQSITITNDKDRLSQEDIERMVQEAADAADEDKKIKEKVEARNSLENYIYQIKNVINDEEKGAKIDADDKKTIESAIADALSWMEVSSTSAEKEDFDAQYKDLEKIVQPIFGKLYQGAGAAGGAEGEMPTHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL69</accession>
    <name>A0A8J4PL69_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ComC supersandwich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009554</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000739">
        <property type="protein sequence ID" value="KAF2069129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL69"/>
    <dbReference type="OrthoDB" id="10520394at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="2">
        <location>
            <begin position="19"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="165" mass="18362" checksum="EF3F4B84F3FEC410" modified="2022-05-25" version="1">MNAALQSTEKVACSYKEFVDDENNSQYLKIQIQDRSLFGRFIKFGMIDGREQVVSNSLLDNVYGGKELSKSTSDQSYIGLNIPFYTKYALLDPDFSVLIEQNTARDQTNSICTNESSKKLTNAQLAGIIVGGVVFLFIIGAVAIYFYTRKSTSPIAMKLRKLGAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL89</accession>
    <name>A0A8J4PL89_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009693</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000780">
        <property type="protein sequence ID" value="KAF2068988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL89"/>
    <dbReference type="OrthoDB" id="21081at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="172"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="25"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="172"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="214"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="251"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="266"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="379" mass="42253" checksum="0A6B99CEABAFEA07" modified="2022-05-25" version="1">MLTQDEHREACRVYMQKYRSKPENREKIRQQNAEYRKRKKEESIRQTSEIDTLESSLDIQTVKINLLESQLKEVTRTLEMYKKEIEIPENYLNVIKDLQDQLSKAYNHIKSQNEIIAQLQQQQTLATTNSPLSSSSPSSSSTSPVSSFYNSQQHFPITNLINNNSNSSIIMAPNTGNSSVNTSPSNSNNTSSNNLSNFFLPKSKKQNTNHLEYSSPPSSPSLQYLPNSPSLSSSPFNMSPSISPTPIHTFNNNNNNNNNNNNNNNKSIASLINPKETNPSASQFSLVPDGSLNLLINNHNSNINNNNNNNINISSSSNAISSIAVLNPNFISKDKIEKPLPSIVKDNNSVKPLPLTLDILCNVLENEKYIENSNGAVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLD3</accession>
    <name>A0A8J4PLD3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009461</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000715">
        <property type="protein sequence ID" value="KAF2069219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLD3"/>
    <dbReference type="OrthoDB" id="17108at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="78" mass="8679" checksum="7444D1F1E8B92BF4" modified="2022-05-25" version="1">MSNSEITVKLVNNKNIILKEGKFKLVNGKLPVVSIGKQFQVKDLIWTDCDYPLVPDKDGLSDMAFTSMQSVNVTGTYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLI6</accession>
    <name>A0A8J4PLI6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008507</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the histone H2A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000530">
        <property type="protein sequence ID" value="KAF2070180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLI6"/>
    <dbReference type="OrthoDB" id="30550at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0158">Chromosome</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="214" mass="24163" checksum="595CCD0842AE8D5A" modified="2022-05-25" version="1">MVRRGRVKPQQYTHRGYHAKHPREARFYKKLLKQVSNNKIRLAVTSSHVLTSLIRNFTKELVDVAIEATIYRGKGTLTARSIQCGLRVCLGGGQLLHFAIVHGMSAVTKMHLKDKPIIPVSYIRNQIKSSLTRLRVAKDACVYLSAVIEYLCFEILELSFNTANNMKLTQITPRHIFLSINDDPELCKAFNKPGTVFVQGGSASFHVKNNNVQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMR0</accession>
    <name>A0A8J4PMR0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome c oxidase assembly protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_008942</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Exerts its effect at some terminal stage of cytochrome c oxidase synthesis, probably by being involved in the insertion of the copper B into subunit I.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
            <orientation evidence="2">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000603">
        <property type="protein sequence ID" value="KAF2069740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMR0"/>
    <dbReference type="OrthoDB" id="1704689at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.370.10:FF:000001">
        <property type="entry name" value="COX11 cytochrome c oxidase assembly homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.370.10">
        <property type="entry name" value="Ctag/Cox11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00155">
        <property type="entry name" value="CtaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023471">
        <property type="entry name" value="CtaG/Cox11_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007533">
        <property type="entry name" value="Cyt_c_oxidase_assmbl_CtaG"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003465">
        <property type="entry name" value="PRK05089.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21320:SF3">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX11, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21320">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04442">
        <property type="entry name" value="CtaG_Cox11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110111">
        <property type="entry name" value="Ctag/Cox11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="86"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="88"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2069740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="322" mass="36671" checksum="58F84FDBB4C295DC" modified="2022-05-25" version="1">MFSRTVSLLSKSIPKSTTPSSFIYKRPSSFIFTAGLNNNNNNVSNNLISLNQKPILSTFNTTSSNSNISIKDTLFLKDILMNNQRLYSSSSNNNSNENKNNNNEKEKQENDNNNKKTFETAEERIKREREEYNQKLHEKNKEIAMYVLAAIITVLGLSYAAVPLYRVFCRVTGFGGTVREQDEFEPMVKRNLDRKVYPIKVTFTASTANKIPWTFVPVQKSIEVLPGEPVLAFYRATNNTDKAIIGVATYNITPQKAGSFFTKIQCFCFDEQRINPHETIDMPVLFVVEPEILDDKNSRGITTITLSYTFFKSNDKGELEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN15</accession>
    <name>A0A8J4PN15_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_008717</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000565">
        <property type="protein sequence ID" value="KAF2069967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057709">
        <property type="entry name" value="DUF7949"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25820">
        <property type="entry name" value="DUF7949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035214953" description="EGF-like domain-containing protein" evidence="3">
        <location>
            <begin position="27"/>
            <end position="1392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1349"/>
            <end position="1372"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="7">
        <location>
            <begin position="438"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="6">
        <location>
            <begin position="656"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="5">
        <location>
            <begin position="804"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7949" evidence="8">
        <location>
            <begin position="1057"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="4">
        <location>
            <begin position="1096"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1024"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1025"/>
            <end position="1045"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2069967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1392" mass="157171" checksum="320E022211D455C2" modified="2022-05-25" version="1">MFKSIFTAIVLLLFLQQNLYFENVQAYQSIDLSHEGYKDMYAVSYELMWTCMTIRMFLFSDPTGIQKFLLLGSWEKSFQLVASNQTSALYRMSLIVPEGATVFQMEITLGNGSVIVENVNIICERTPQMDIEIINPTLQYYPSLVEGHPHFFFKVKNYNKIISPPVRVEDPLSNYKVRVNAVHANIYIAELSFNTVTISNYKSNSDFSFKNSDGFGSKWINFNSFLQGYQNTEIYSDLNLYLTDGDGNFDSPELLVFSDLEMQENIYPFIMYSYNSSVISPVLVLGNFTNPMFYSKIIVQPREYPTSLTNFYSWTFQTNSPNIIYKPFAYTGFTSNPKVENPFFGLFLNNLFCLQYTTNATYRSDLVTFKSNTWIYLPYPFGYSKGNSNGYFKIIEIFSDYRYLPLMVEIDNQIPLTMDYILNNGLIEFDGAGPNVEIESIESYQLPNSPWLVLRMRITDDLSGFSKTEFGNYQDIIQGTINDGVYEFLVLQSNPITQISKGDLIFKDSIGNSRQYNFMKYIYTSTNLDKFPLKSLNFSNILSIRFEKNDINVSNQFVNNNMLIYTSNVDKTIYPGILVFKYGMGFDRESFTPYSGSWNNTCNCYVIPFTVPMNYPTGHFEFQMSFDSFTNWDCLSQYLITYFGPDAGLRVYSENIDLIGPMVNQVDKTSAAVLVPSGVDTKISWNISLMDQFNGFKTGLVSVVSSLDLVRYNFTITPDKVVIQGKNIIFNMEITVNGKCSSQSFYIQYLYLEDNAGYFSEYLEGGTYSETQSINPFLLNIQNITKYTEIQVVCENSIIDTTEPTLVTFQFSPSLLDVTYTAEYFNTSRVVNFQFTTQDDNGILLASKPRIYLQDKRYNFIYQDARLTVSSLTTAQYDCQFKIPFGFGFGGSIMVSVFGIVDNKSNFNGYSSSQLQARGLPYEIGVVQQFNNTPAILSTRPVKGSSQDITIIGRNFDSSDRLFINFNNGSIINYSTCIFNTSSIIIFNSIGHIFVPNITITIQKASRSISNSLFIRISDPFVPKSDSSNSIDSSSSNSIDSTSSSAPTTNKPYSCIDNCGGTEQGVCTTNGCICKSPWVGISCSSKVIIIDPVVNTTSPSTNITVPNNDRDTAIYYAIISVVGLNELDRDGNVVTTHSLTKWIASTLVSKYFSATNSSHFYSSSLLQNNVTTNVNVSIDYFNQPLPVNITFANQIFEMNPYSLKYSINISEYSFSSSFNSLQLVLLASIESNLQDECSSKIFQDTVESGSEYVKMQVNDHSLYGRFIKRGIIDGRIKTIDNVLLDSQYNSVTSESKSQSYIGINIPYFRKSVEIDPDFSVLVDNRPASSESENAICAPHDKSKLTKAQLAGIIVGCVAFASIIIASVTYAIIKTKKQKQFKKNIQQKLKVLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN20</accession>
    <name>A0A8J4PN20_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="4">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_008667</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000557">
        <property type="protein sequence ID" value="KAF2070010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN20"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004104">
        <property type="term" value="F:cholinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000997">
        <property type="entry name" value="Cholinesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45570">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45570:SF1">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00878">
        <property type="entry name" value="CHOLNESTRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035341939" description="Carboxylic ester hydrolase" evidence="4">
        <location>
            <begin position="22"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="5">
        <location>
            <begin position="37"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="3">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="346"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR600997-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2070010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="539" mass="60309" checksum="EBBF3EAED12EEBBE" modified="2022-05-25" version="1">MKQQYYILLFIVLLQIHFINSFDFINENQNQINLDDPSIIETSNGYVKGNLNDNYMSFYGIPFAQPPINNLRWQAPVAVSDWAPQVLDARYPQFGCPQVCELPPGTCPTNTSESCLFLNVFTPIQDSNQGLRPVMAFIPGGRFEQGAASSPLYNAEYMVNSSNAIVVTINYRLGVLGFLLTDDFQGNYGFQDQRQALTWIQNNIKYFGGDPNQVTLVGQSAGATSIAAHMTSPLSWNLFHRAIIQSDPFTLGLKTKEVAQEFSIQFTQAVKCSFDDTKCLLNLDVATILDGQVKAQQTINPLQPLVSFLPWTPTVGGSDIIDQPYSLIQKNQFYDIPLIIGTVSEEALLFIYQAAPKPVSGLDYEVVLDLVFLKNAEKVNQFYPANITSDNRPKLSVLGTDYIFVCPTKSVASYLSQYKTTPTYFYQFSHVISVDVWGPNYPYCVGHVCHGSELPFVFNTATSTGYVITSAEQQLSYTMNNYWMNFVVNSDPNTGLAVPLEWPTFNTTTSMNINFQTPPFIESNLLNSNCNFFDSIGYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN65</accession>
    <name>A0A8J4PN65_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Proteasome alpha-type subunits domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_008457</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000523">
        <property type="protein sequence ID" value="KAF2070223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN65"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03754">
        <property type="entry name" value="proteasome_alpha_type_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000055">
        <property type="entry name" value="Proteasome subunit alpha type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034642">
        <property type="entry name" value="Proteasome_subunit_alpha6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 8" id="KW-0647">Proteasome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="9">
        <location>
            <begin position="10"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2070223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="246" mass="26729" checksum="F0DBD6CF2B346725" modified="2022-05-25" version="1">MSRGAGTAGYDMSIIFSPQGKLYQVEYAFKAIKTGGLTSIGIRGKDCVVVATQKKIPDKLIDPKSITSLYKLTDYIGCVQTGIVPDSKAQATKAIQEAASFHHKFGYQIPPNALAKRIADIAQLSTQHVSARPLGVSMILIGIDDELGPQLFKVDPAGVYSGYKATGAGEKEQEATNFLEKKFKPNPQLNQDETIQMAISTLQNVLGADLKASDIEIGMCTMENRKFVQLTEAQIDDQLTKLAERD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNZ8</accession>
    <name>A0A8J4PNZ8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008657</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000555">
        <property type="protein sequence ID" value="KAF2070026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNZ8"/>
    <dbReference type="OrthoDB" id="10614282at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="215" mass="24874" checksum="24E134C8B048F0EB" modified="2022-05-25" version="1">MIHLNILIIGLPRTGKTTLSNGLYEHPLNRKDAILSELKKNFTNVEMKYILNVDSTSNIEEHQILSEREEVKRQFDLSKYDFIVCLIDKTNLLSWHFIRDYLLKQNEIDITLFTLNRICLVVTKDDTPTQTSSVSMAEINSFSKYLSIRLHCSNLVDPSSLYNLTDKIMTQIQLSRSKSVYYVNSYPTITPNLLLLDTTDLPLPKSTKIKQQENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP04</accession>
    <name>A0A8J4PP04_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007885</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000445">
        <property type="protein sequence ID" value="KAF2070794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP04"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="107" mass="12284" checksum="913E614A50EC9E08" modified="2022-05-25" version="1">MDSDSNTIKQTNADRLVFDNGMTYSQCSNVAVENIYVNYNIIENEDLIDTHCGNLETRPIQVEKRYVLNQLNQEKLLSVEIKTSTPLFVTKCGFQDLDSHSFRVNSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP72</accession>
    <name>A0A8J4PP72_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lipid-binding serum glycoprotein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007758</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000432">
        <property type="protein sequence ID" value="KAF2070914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP72"/>
    <dbReference type="OrthoDB" id="16440at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.10">
        <property type="entry name" value="Bactericidal permeability-increasing protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017943">
        <property type="entry name" value="Bactericidal_perm-incr_a/b_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31138">
        <property type="entry name" value="CHROMOSOME 19, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31138:SF9">
        <property type="entry name" value="LIPID-BINDING SERUM GLYCOPROTEIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55394">
        <property type="entry name" value="Bactericidal permeability-increasing protein, BPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="566"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="572"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="593" mass="68230" checksum="548FE4E3FDCB4290" modified="2022-05-25" version="1">MVNNRKEQDLVDNMEEEEEYEEEEEEEYEEEEEEEESYDSDSDEQVEYDNYKEKEEETDIKDELLKTIDNKEMIQNGKELIQQSNVLLKEIKELESGKDFYESGKVLVDTVLKKQEAKELMEKGLAILNDAKENKEAQVLINECKQFIDLFSKEEKVRTLISQGKDLLDSIKANTNDPSSPTFDKNKIEALFVQGSELFVDFKENEQNSLYLDKMKKMFFDLKEAESTRITISKGIEEGKKWVESLKEKDPQIKELVNEGEEIFEQLKNNQEVSSLWDDSKKVWSSFLADENRSLATLDSPDVKQLIERGEKIAGELKETLKSHKIVEEVSNRQDVKLFLEKGNKFIHEVKSKEQLIDFLKQNKDFIKEIKNTIVPFITDQLLKVQIPVVNGVTKGFKYEISKLVFAGLGIPPEGVDVNFSETNTITVSVSDFTASLKNFAWSYKQEGFPYLKDTGVASANVEKGFSSISFDIDTNPETNTPEITITDITFKINHLEVIVDEGKVKWLYNYLISKFSESIKDVVEGKIKNTLTGHLNTLTTKINQIVGQYWSKLIQKLNNSDDSKIKEIDDNNNNNDNDESLNNSIKTDLVLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP82</accession>
    <name>A0A8J4PP82_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_007027</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRM CPSF6/7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000353">
        <property type="protein sequence ID" value="KAF2071652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP82"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034772">
        <property type="entry name" value="CPSF6/7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23204">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFIC FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="159"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="253"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="372"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="256"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="292"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="314"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="372"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="388"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="415"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="436"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="608"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="661"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="698"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="709"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2071652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="746" mass="85351" checksum="AED75CE0CB197811" modified="2022-05-25" version="1">MDDDDIYGEFKTTNTSRDDSDHDNSFTSTTTTNITSTTTPPTAVSTNSTADKQDDHHKETENDSTDQQKESHSPSPLSSSSSDQNKKDINMKDSIEYESTDSSNLNSSNTSIMNNNNNQIQTIVEGDMDSSFDETEDSISFSEETIDNQNGNSNPDQAPAIYIANLEWWTTDQQIEASLSEVGKPKTLKIFDHETSGKSKGYAFIEFNTVDQAQLAKEKLTNVQLNGKELIVKSISLANYHNALDNIKNLGMKPMQQPQQSSLQQQQQQNQQQQQQNQFDSSSMRGGRGGKRGGMNNRGGRVGGGAPEYYSNQVGGGISARGGRGGHFPPGGYMGGRGGHYPPNPAMGRGGLMRGPPPPMIYGNHPPPMIYGGHPPPRNMPYPPPNGRPEEWMESPEHHYGSPDQSHHHHHHHSNSNNERSSSSSSSSSVSTSTSNEKRKRDDDFDERERDRDRERERERRDDRDTRDNRDRDDYYNNTRDKERDKSRDRDRSDRDRRDERDDYYSRDRDRNDRDRDRDRDRDYYREKSSTSDEKSNRSSSSRDKEKSEKDYRSSERDKDRDRDRERERERDRSKEKSEKDKESSSSSRDKSDRDRSYESSSSSSSSSKDKDRKEKDHRSSDRDRNERDREKEKERNNDRDKDRDYRSSDRDRDRDRDRYSSSGSGRSSSSSSKDLKEDGKSSSSSSSSNREKEDSKSSSSSSSSSSSNRDKRSSKDQEKIDIEREKERIRERTKKFSTKSSSSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPI9</accession>
    <name>A0A8J4PPI9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RNA-binding region RNP-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_008260</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000494">
        <property type="protein sequence ID" value="KAF2070416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPI9"/>
    <dbReference type="OrthoDB" id="6275295at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12446">
        <property type="entry name" value="RRM_RBM25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1390.10">
        <property type="entry name" value="PWI domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002483">
        <property type="entry name" value="PWI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036483">
        <property type="entry name" value="PWI_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052768">
        <property type="entry name" value="RBM25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034268">
        <property type="entry name" value="RBM25_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18806">
        <property type="entry name" value="RBM25 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18806:SF4">
        <property type="entry name" value="RNA-BINDING PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01480">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00311">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101233">
        <property type="entry name" value="PWI domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51025">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="625"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="165"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="PWI" evidence="7">
        <location>
            <begin position="540"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="252"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="379"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="494"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="286"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="344"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="257"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="495"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2070416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="671" mass="76805" checksum="ADCA33366FDB101E" modified="2022-05-25" version="1" fragment="single">MHNPQGGPPPFMGGPPPHMMGGPFHQSGPPPHHHAGFMPPQHGPPQPFPNGPPHHHAGFMPQQGPPPHMMVGPPFNGPPGMHPSLHNRGPPPQMGGNVPPMMPHHPQANLPYTSKISPPIHHPQQQHHIHQQGPLIHHQPPQLHQINNQPIQHQPQQQQQQVVKTIVYVGKIPPLVDDSFIKQLLDQCGNVTKWNRASEPSGKLKGFGFCEFESPEGALRALRLLNDLEVEEGKKLLVKVEDKVQKFLNEYMEKKTTSPSNDESSETDTSTTNDESIKETIKSMLEKNLETLLEKKKLEMIQEQQNNILNNLNNNNNNNGNNNYNPLNDFYNKDRKELTKEEKIKVIEKEKEKEKQREKERELKRKERDIRDFKDKERTWEKYSDSDEKQRKRIRSREAVKHRQREREEDEKEIAKEEAELIEQQKQQQLNEEKHQKLMDESLNRIQLGFNVKKSKLVAKGFSTLDQDDEESVLSSTRKKQTFIPLNFENDILKNSNNNSNNQNQSNNSILTSPPPNSAIKQKSKEEIQEIIKQIPNEKEEIFKMAINWLRVDQLNIIDDHMKPWITKKVTDFFGGQEDDIIEFITNLLKSKTPPEEFITKLQDVFENEAEVFSNTEKMMKVNKLLLIAILLAAVGILIAEASSAKAATATKTQPIGCQGMKCQAQKLVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPL5</accession>
    <name>A0A8J4PPL5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007485</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000402">
        <property type="protein sequence ID" value="KAF2071199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPL5"/>
    <dbReference type="OrthoDB" id="441210at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000795">
        <property type="term" value="C:synaptonemal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042448">
        <property type="entry name" value="CCNB1IP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14305">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CCNB1IP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14305:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CCNB1IP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="20"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="171"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="238" mass="28133" checksum="93BF4FBDBEF7C433" modified="2022-05-25" version="1">MVGKSIDFWNYQHSIYIETILNHTIELKNQQNQFKKVLEEANLKIESLEKQLKIQKHEHDNNQKEIVELTDKLAEKTKQKRKLEELYTSLKENRKIFHNNHTDNQEIIDHSNNRDNDFSYQTNLGLKFDKNQGKLPNFFKKNQGDSSARMERLSPIDKHILKQSPIKSNFINNNNNSCNRSPNINKTKSPLNENSFYQDKRVIMSTPQKLIPKTKPLLDKFSMIHKNTPVRKTPTNYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPX6</accession>
    <name>A0A8J4PPX6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_007272</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="AJWJ01000378">
        <property type="protein sequence ID" value="KAF2071408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPX6"/>
    <dbReference type="OrthoDB" id="10254671at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14132">
        <property type="entry name" value="STKc_CK2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000059">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000088">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045216">
        <property type="entry name" value="CK2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="47"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2071408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="337" mass="39896" checksum="BA7FAA99A794DD2C" modified="2022-05-25" version="1">MNHSSKKNKNRILRNKARIYCDVNLHKPKEYWNYEALNVKWESQDDYEIIRKIGRGKYSEVFEGFNLKNNEKCVIKVLKPVKKKKIKREIKILQNLCNGPNIITLLDVVRDPQSKTPSLIFEYINNTDFKHLSPTLTDFDVRYYIKELLLALDYCHSNGIMHRDVKPSNVMIDHQKRKLYLIDWGLAEFYHPNQDYNVRVASRPYKGPELLVDMEDYDYSLDMWSLGCMFAGMIFQKDPFFHGHDNIDQLSRIVKVLGTDEFYAYLDKYGIVIDQTLQSIISKHPKKPWSRFITKDNAHLAVPEAIDFLEKLLRYDPAERLTTKEAMEHPYFKPLLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQI7</accession>
    <name>A0A8J4PQI7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006576</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000310">
        <property type="protein sequence ID" value="KAF2072102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQI7"/>
    <dbReference type="OrthoDB" id="24030at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35885:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35885">
        <property type="entry name" value="CARBOHYDRATE BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035223394" evidence="1">
        <location>
            <begin position="21"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="310" mass="34958" checksum="B16BF14E6CA09627" modified="2022-05-25" version="1">MKFIIALLVLVLVGISCTRASPQPSVFIWNEGFDLKIGVADLPSDNKASVKLSIPLVYYQDSTTQSSTYNYATKILTFYGLHYADAKPILYVVDCNTWTIVSKTGVPVEKFQYLGLASTNTSSNGLFTTVSDGNFVYPSFIDNENTINIMDAASGSYRGSVYDPNLESYFVAFRNTTGLFMRVYHVSNVFITEFEMEFTNNISPVNDYPLNMFYNPSNRAVMAQVSMKDDNKNTFFSLAYFDWENQSFDVTRMVAVHNGQLVTSFVPVTQSKAYSFASVRSDYYQIYSFDTVLNQFMSYGPFYTPVSSAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRA1</accession>
    <name>A0A8J4PRA1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="11">1.2.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_007218</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = (2R)-3-phospho-glyceroyl phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10300"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="11">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 11">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.12" evidence="11"/>
    <dbReference type="EMBL" id="AJWJ01000372">
        <property type="protein sequence ID" value="KAF2071465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRA1"/>
    <dbReference type="OrthoDB" id="13738at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00184"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004365">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18126">
        <property type="entry name" value="GAPDH_I_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05214">
        <property type="entry name" value="GAPDH_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000001">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000020">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006424">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01534">
        <property type="entry name" value="GAPDH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836">
        <property type="entry name" value="GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836:SF76">
        <property type="entry name" value="GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00044">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00078">
        <property type="entry name" value="G3PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="4 8 11" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding" evidence="12">
        <location>
            <begin position="3"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="12"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="150"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="210"/>
            <end position="211"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Activates thiol group during catalysis" evidence="9">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2071465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="334" mass="36097" checksum="F6B5FDAE5719A544" modified="2022-05-25" version="1">MVVPIGINGFGRIGRLVLRSSLCNPECQVVAINEPFMDAEYMVYMFHYDSVHGRFKGTVEAKDGNLVVNGKTIKVFVEKDPANIKWSTAGAEYIVESTGLFLTTEKAGVHLKGGAKKVIISAPSADAPMYVMGVNEETYKSDVHVLSNASCTTNCLAPLAKVINDNFTIVEGLMTTVHAITATQKTVDGPSGKDWRAGRSALANIIPASTGAAKAVGKVIPALNGKLTGMSFRVPNADVSVVDLTVRLEKPASYEAIKKVIQEAAKSDKYKRILAYTEDEVVSSDFIGDTHSCIFDAKAGISLNDNFVKLVAWYDNEMGYSTRVIDLIMHVAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRR0</accession>
    <name>A0A8J4PRR0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_006780</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000328">
        <property type="protein sequence ID" value="KAF2071901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRR0"/>
    <dbReference type="OrthoDB" id="430364at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:001049">
        <property type="entry name" value="Ankyrin repeat, PH and SEC7 domain containing protein secG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1000.11:FF:000003">
        <property type="entry name" value="Brefeldin A-inhibited guanine nucleotide-exchange protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000700">
        <property type="entry name" value="Probable serine/threonine-protein kinase DDB_G0272282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173">
        <property type="entry name" value="ANKYRIN REPEAT CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173:SF74">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="71"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="104"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="170"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="203"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="236"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="269"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="302"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="336"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="369"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="402"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="435"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="468"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="501"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="SEC7" evidence="6">
        <location>
            <begin position="582"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="784"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="914"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="32"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="920"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="938"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="956"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="975"/>
            <end position="984"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2071901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="984" mass="107871" checksum="41C293329DEC7882" modified="2022-05-25" version="1">MGTTTTTNTSSGTSNNNSNNNNNNNNNTSNNKYTSAPTRPSVHYYSSTGDIEKLSNLLNNSATSADLQDSEKRTPLHHAAFCGSAACVNFLLDKKGNPNAEDAAKNTPLQWAASRGHLECMKLLIERGAEVNTKDDKNGTPLHKASFFGSSECVLYLLQCKADSKATTINGETPLHHASAGGNPQCVELLIKADSKVNAVDNDGITPLHQASFSGHSSCVSLLLRKGAKVDPRDIHGISPLHNASSAGYIDCVDLLVRNGENVNCVDIEGVTPFHHACFNGNLPLLKRLVELGSKIDMIDEMGETPLHKAAFNGHRDIVEYLLQLDPTMIDARDTRQSTPLHLAAFNGIYDIVELLILKKATINIKDEEGATPLHKASFNGHSSCAKLLIDSGASIQILDNQGASPLHKAAFNGRSKCLTTLIRSGSDLEVKDNQGGTPLHNAAYNGHSDCCRILLKKGANVDAIDTHQSTPLHLASAAGARDTVDVLVACKAKIDPKNCAGKTPLVYAIKKNHSDVARVLIRAGADLSSVSSRSSVDLSKIFGTCNLEEIYQIVNKRESSIIEIDHVQNALDLQQAKEDQELQQEKAKRVLIKNAVTNFNTHPRKGIEFCILNNLCERTPKDIARFLLSTPELNKQAIGEYIGDGDEFNIQVLHSFVEQLDFTGLDFDIALRKFLFNFRLPGEAQKIDRMMERFAQQFYQHNPDNKIFANHDAVYVLAFSVIMLNTDAHNPNIKKKMTKPEFISNNRGINSGDDLPVEFMESVYDRITTNEIKMERDGSANQHVEKKGWLTKQGGRIKTWKKRWFILTANCLLYYKTPQDTEPCGIIPLENVVVTVVVQKKFCFMLHSSQEQMKACKLNSGGTLVQANHAAYFISAANQPEMESWVQSIKSNIHSNPDFELLHKKKAETIRGRGKINKPIQTRKQTISGAPVGNPNHGLSQNTSNNNMGGSNHITPSSTPNSTSPLSSNLKPVTFSSTASPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSQ2</accession>
    <name>A0A8J4PSQ2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_006159</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000272">
        <property type="protein sequence ID" value="KAF2072516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="427368at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005697">
        <property type="term" value="C:telomerase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003720">
        <property type="term" value="F:telomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070034">
        <property type="term" value="F:telomerase RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041452">
        <property type="entry name" value="APAF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025139">
        <property type="entry name" value="DUF4062"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045804">
        <property type="entry name" value="DUF5920"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052652">
        <property type="entry name" value="Telomerase_Complex_Comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008858">
        <property type="entry name" value="TROVE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037214">
        <property type="entry name" value="TROVE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44791:SF1">
        <property type="entry name" value="TELOMERASE PROTEIN COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44791">
        <property type="entry name" value="TELOMERASE PROTEIN COMPONENT 1 TEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17908">
        <property type="entry name" value="APAF1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13271">
        <property type="entry name" value="DUF4062"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19334">
        <property type="entry name" value="DUF5920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05731">
        <property type="entry name" value="TROVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140864">
        <property type="entry name" value="TROVE domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50988">
        <property type="entry name" value="TROVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="TROVE" evidence="7">
        <location>
            <begin position="65"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT" evidence="6">
        <location>
            <begin position="974"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1478"/>
            <end position="1519"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1520"/>
            <end position="1561"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1606"/>
            <end position="1641"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1698"/>
            <end position="1739"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1782"/>
            <end position="1823"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1825"/>
            <end position="1866"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1864"/>
            <end position="1895"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1906"/>
            <end position="1947"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1948"/>
            <end position="1989"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2066"/>
            <end position="2088"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2087"/>
            <end position="2128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="219"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2072516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2362" mass="264841" checksum="4B72C286784656A2" modified="2022-05-25" version="1">MSSGPRGVVLNRGGAARGGRGGRGGRGGAAARGGRGGASARPPREFSADQPFEPKVYKKQSTFFKELKEKKIAIVGSEELKKLKLELINLVSASMIKEPRINQLTPTLETIMTLVYNISFFDPEFILKLVLYTRDELGIRTISNYLICLSSLISTTQPFLKKYFSQCIKIPSDWLELPNIIFNLTDSKSIPKSLRNVMVQKFPDFDTYQLGKYNTEGKIKRNRKKLKEMIKKNPELKEELEKKSANNQVTSLKNMIRLLHISEPVYSVMSILGKKYPASYEEFMKSKLPGQYESERAGKKMRLPIPETWETLVSEKGNKASTWEELIDHNKLPFMAMLRNLRNMMITGIQPRHHNWIIKKLTNEQTIANSKQFPLRFLAAYDNIPTDQKQLLEWAAAKKVKVAYSPSNDLFKNYRAALDTAVKLSTTNNVKPIRGTTVVFCYADLEDQDICKSNNAGKYTMTESAMLLSLMCKYVCEDCQLFLVDYKHADKQVYIPVNDFASDSILENIKSLKEIVSTQFKAGHAVYEARFPTEFLIQNYLGPQVKVDNILILNSTTIQLDREVYNRNVLDKYRQDVNPELLYVSINLSGKTDNSVATGANPPPHQNDVYISGFSDSILKFIAERGGSDQQLQYIEQIDTIKKIPEIVAKKLSPKETLSATDLELENQMSQVDLNSQSVATANSIVERPWRTAKVFLSSTFLDMHGERDLLVKHIFPELRQRCLKHRIHLTEVDLRWGITEEESAKNKSIDLCLDQVDRCRPFFIGLLGSRYGWTPRRDQIPADSKYDWIRNLQGTRSITELEMLYASNLYNSSTPNNNCLFYQRDNSFYATVPKEFKNQFECEDRESATKLEALKSKLIKSGVANYTYSSTYHGLIDQKPSAGQLNDLHQRILTDLWNRIVSVFSLNTNSASNLLNPIDIIQEEIDNHLNYAELKNRHFVGRKDEINCLYKFADISRPFSSTKANKLVFNKSNTCLVVGKPGIGKTALLAQFANRSIASAFEGKSAKIARPVVIQHYVGSTSSSTDIRKTLINICNQINIKLQMNETIPVEYSQLKIAFGKILKKASIKNSKLVIIIDGLDQLDKSNRANSLDWLPTNSPVKLIVSTADGDPTLQVLRKRRVAPTEIVLPQLEQSDRYSLVKAKLDEYKKKLDESFNNNQMKMLLSKTDASNPLYLMLCCEELRFFGVFENLTDKIKSLSQTLPTLFDDIMTRLENEHGKVLISNLLGIIACSRVSLYEQDIRQLLARKEMNEAYLPTSIWSRVQRSIQPFLIDSVDKDNDSEIGLEFFHNQLKIAVLKRYHSISEVSIRIHKLLADLYTTRADPHHNASWNGKDSTAFSELPYHLLKARLFTTLDNTLCSLKFIESKCKFGLTFELLNDYLEILDVGEKVTLNSPIKLIKSKDTIAHFNDFYTFVSANTHIISKSPNLVFQQAYNQPSSSYCHKVAKESIPSPNSNKPWLEYLNKPQEKSNCKQTITGLQDGATAVAYSRDGSLIASAGKDCIIRIFKALNGSEILTLTGHTNWISSFSFSEDNKTLASSSWDETIIVWDLVVGNQIAKISDHNRAVNYVEFCPIDKNILLSCSWDSSAMLYNLYDKKIHRMFKNAHSKPINACCWSNDGTKFATCSWDSSIKVWNATTTLFNERLISTIKLDASVATSSFKSIKFSANDKQIIATTMSGSVMLFDIGSSRFISEIGNHSKSINHCAFSDNGQYIATSSDDGTVKIWGSQLGQELNTIQLEDGWINCLVINKNIMAAGCSDCSVRLYDIGLKVPRLLAILKGHTRSISGISLSADNTKLASTSEDLSIIVWNLATNKILYTLAKAHNDPINSVNFNHDGTLLISSSDDYSTKVWNGTSFKLLQSNTNTIKYSTFSPNGEYIATVSRDKSITVYLTSSLKELFKLKGHTDWINFCTWSPDSKKLVSGGWDFNLRVWNIKKRAEVLCLKGHQGSIEKAYFTPDQKYIISCSFDGSVKVWDASVGSEITSLSHKARISDLALTADGKVFSCSDDGELKSWSPVSGKCYATLTGHSGPIKQSVFAPSSGSGFFSSFGSGNETATPQTLVSASFDGSLKVWDFNEKSSVAPPHQGYINCCALSKDGSKLVTVGQDKLVHHWNVSTRQIEKTQTLDSPAVTCAITSSGSVFLGLENGKTFIDGVEGTDFKTKVTASVSYHGDDSDVVLLSGWDRNIRVYATTAESCVNLHVAPDWVECLDLSADGLTLAAAGRMNEFYVIKSQDLGTANYATPYTCSTTAVALSADTNEVALGSFNGEIYIFNRSTYQSRLIPTSTKYSITKLHFLDKHRLLATSLDNSLLVINLLTNTIDTEFVHNSPVTFMSIHKNLLVTTDSNGQVYFLNLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSS5</accession>
    <name>A0A8J4PSS5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide-like helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_006136</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000269">
        <property type="protein sequence ID" value="KAF2072561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSS5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="497"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2072561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="764" mass="87981" checksum="2B5CE32F4D3E6F12" modified="2022-05-25" version="1">MTVTNHISKAFDKSGIRNTVYDLISQQAYGTVNGKKLTYETNQSRLEHDFKTGVREQFAIGGEFPTCSKGKVTNIDLYPIDFEKALPLTQLNNFDFKIPDQPICFQDKTNIVYIGPSKDLKKKLEIYYFKFFTKSYTIASQYQKFMSETNWQCKGLDQKDVKSNAFFITKSKNVKKIKDSDFHHITFSFNYNTLGGRSSTVSIFRNNNTRALLSSCTATIDHSKKSINTHVNNNNNNNNINHHKSSQSLFYNSISLNSNFSIRTFISSNNNNNNNNSNNEQQIDTKYQSYIDNAIDNFKLKQIDLTLEFIDRAIKLCPNRADAYLLRADINHNLLLEEKNSSINDYKKALQLLSSSAPNISLSILFSMLELSDTPNEIKEYIDKIKLIDPFNKKIMTDYNDDKDRKLAYAKDLFEKGDNYGTFLLASIAKEMEDYSKAEPLFKQVIELEKQRLDTMLIREPQLIHNRFIVDEIVEDDGTVYKRIIEYDGTEEKKTLFLAYSGLGSIYVTLDRLIDAFDNFIAAQQLEPNLSVFSFIFSRLLFQTGHYEEAYAYLEQSLKSDQIFAGKTADYKRSLKADILIALGDYQHAVFELLTVFFGLLKKETDSKEESQRLTKLMSLMLQLFSKLFSLSPATAAPEIKALVDPFLIKHDPGSLLATPPQSMTDMYNQSEILNSVYNDLFYESGIIHNSSVSPQTLASIRTRAHFFDYLYYSLRDSSALVLFKNPLLTIEEFEKSLDHLSPQRKEVHHEQFKVYKNLFPKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSW4</accession>
    <name>A0A8J4PSW4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005815</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000242">
        <property type="protein sequence ID" value="KAF2072867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSW4"/>
    <dbReference type="OrthoDB" id="20474at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15551:SF3">
        <property type="entry name" value="LIM AND CALPONIN HOMOLOGY DOMAINS-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15551">
        <property type="entry name" value="LIM DOMAIN ONLY 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="427"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="332"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="443"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="517" mass="58552" checksum="EA2AF259984C0C94" modified="2022-05-25" version="1">MESYSPSIQKLIVFLKPQADITNNGIYYLNNLLTDISNSIIIESINNSKSHLQKTILSEDVEKALFKIFPSDVADSIVNQAKGRIQHTSMINLSNVKKFEKEDDEKNNQDNNIVNNNSSGESKQEQQLVIDTDDSDVDDFEIINNTNTYNNDLSLYFPTDLVYKVTSDFISRKKLIQQNAIEFYKVLEQQEGNNIIYTFISYAMESITTMILLKTLIIDIDEDAIRKTIQDTPALNQIRHNSIQITNITNRLLNGESIDSITSSASISSLPNFLSQIDSEEDDDDLNLLSNSYISSNFDIINNDNDVENNNNNNLDNNSNIESNVENKIENSDNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNRIIIDNSNGNNNNIDNNLNNNVSTSDGIDNSNKVIDQNLIQQIVQNQLEMMIDKVVEESPSSSKNIVKKRNNDKQSQQETLKQQSQQSTISCSKPIQNIPNLFTFSQPISLTQLLYNGIHMELKNGMFLVVLILFGLLIQGANRDEIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTH1</accession>
    <name>A0A8J4PTH1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_005473</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000219">
        <property type="protein sequence ID" value="KAF2073222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTH1"/>
    <dbReference type="OrthoDB" id="21392at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF519">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE MPK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="8">
        <location>
            <begin position="206"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="9">
        <location>
            <begin position="374"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="247"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="468"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="140"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="70"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="251"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="288"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="468"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2073222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="543" mass="62761" checksum="1A47ADC7BD54DF6C" modified="2022-05-25" version="1">MDRFLIKVNNNNNTSTTPSTNNNKNNNDTTDGPKIKDENNNKQINDTTKTSDTKSTPAPPPSTPSKSKKKKDEKPADKDKNSKSTNSKDEKVKDTKDKVDTTTTSTENEPPKKNQTLINFFFKPVNNGEDSQPAWKYLFEKEAAERAEEERLEKIRLEKEAEILAAKKKELAKKKAAMKKKRELERQEREEEEYQEYMAMRERTTKGAEIIPSLYLGSAIAAKNAEWLLEANIKKVVNVTSEVSCFFKEDDVIEDSQDEEEDDDEDEEDDDEEEEEEEQEEQEEEETKGEMEKDDKEQQVDTKENQDDKMDISQPSSQIGQDNEMDQSKDQQKMEIKPDITNDDKKQQEDVDPSIPIIQYLRIPIHDDSKAKIETYFQQSSDFIGDLSEKVLVHCQMGRSRSPSIIMAYLMMVHKWTLERAWNTITDKSVKPLTVNDGFKKKLMDLELSLFDSNSIDFFSKAQRVRTTINNNNNNNSQSTTPTNPTPRKLRKAKDSKKAQQEQDEKEEKEEKDGMDTSENVNNIDDFKVVKRRKPNSILEEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTI5</accession>
    <name>A0A8J4PTI5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD-specific glutamate dehydrogenase</fullName>
            <ecNumber evidence="4">1.4.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_005626</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">NAD(+)-dependent glutamate dehydrogenase which degrades glutamate to ammonia and alpha-ketoglutarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-glutamate + NAD(+) + H2O = 2-oxoglutarate + NH4(+) + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15133"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.4.1.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.2" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000229">
        <property type="protein sequence ID" value="KAF2073071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTI5"/>
    <dbReference type="OrthoDB" id="184415at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056365">
        <property type="entry name" value="NAD-GDH_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016210">
        <property type="entry name" value="NAD-GDH_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055480">
        <property type="entry name" value="NAD-GDH_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF24">
        <property type="entry name" value="NAD-SPECIFIC GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23147">
        <property type="entry name" value="GDH2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23152">
        <property type="entry name" value="GDH_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000184">
        <property type="entry name" value="GDH_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="5">
        <location>
            <begin position="667"/>
            <end position="931"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2073071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1027" mass="115821" checksum="5D22A092F92BC239" modified="2022-05-25" version="1">MNQNLTISQEIAAQPKTQYFTKEDGDALNALLEQNPFKEQAYEVKQLLKVERLLEATRVDPEVDWFYCKLGIDGNYFESTPAVVVARHILSLYAAKMVSHATGAKLEVQLHSKTEGSATFITPSNPGKRDSPAMAIEHSIEAYYFGEGYHQDGMGSHSLVQSPSTLHMMSPKPPAIPSPHGFRLACYRTTGTVSNNSPVHLRLYYLTKPLFAEGNDHISPTETDLYKIGDVSFLEKSSDHTKKVYQEVMNEVVTKQGPVIKFYPYQTNGARLVVAYRRGSTHSYWSAISELYHFHQMYATHKYVEQFSNGIVIYSIYLRPLHPDVDINTKIPKIAEQASLVYVLPRTSLTSLFLTHQLSFPEVTYAYVGWKFAYQFLNRYATEYSALAHAIGDDSTKQSMLAQLKTRLSKDTFTEGRVRDAIMQYPELIKVLYQDFEKYHFQKSSAAKYDTQHGADILHQIKRTVNNELDGQIFNAILSFNRHLLKTNFYKNTKTALAFRLDPRFLSTKEYANCPYGLFFVVGSEFRGFHVRFRDIARGGIRIIRSQNPTQYDHNSSSLFDENYNLASTQQSKNKDIPEGGSKGTILLSADHQAKAEVAFQKYIDALLDVILPNDEIVDHYGKDEILFLGPDEGTADFMNWASQHAKDRGAHFWKAFTTGKSLSRGGIPHDLYGMTTRSIHQYVLGTLKKLGLEESSCTKFQTGGPDGDLGSNEIKISKDKTIGIVDGSGVLFDAQGLDREEITRLASKRQMARYFDKTKLSSQGFFVDVQDTDAKLPSGEVVESGLAFRNNFHLNPLCTADIFVPCGGRPESVQLTNVDKMFNANGSSRFPIIVEGANLFFTQKARLMIEEKGAVIFKDASANKGGVTSSSLEVLSALSLNDEEFDKNMCVKDNVIPEFYSAYIKDVHRTIEENARLEFECIWSEHEKTKTPRSILTDTISNKINSLDDSIQTSPLWDNLSLRKKILSSACPKVLLDFVGIDKIMERVPVPYLKAIFGSYLASRFVYEYGLSSPEFAFFTFIQKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTM6</accession>
    <name>A0A8J4PTM6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000191">
        <property type="protein sequence ID" value="KAF2073658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10486280at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1406" mass="163558" checksum="BA26F2B9FC1260DA" modified="2022-05-25" version="1">MSFKSTSSNSSKISDLINQIHESNGDKSKIENVVKSIQVLYKGNEDKVSQEFREQFVKWVKSKSIIKPKIMVDPVVKQNENEKKHESENSTPTNESEYGDEPEKPFYLQDDEFDSSFDDDEEQLSLSVEEQNIYIKFFNQIDLFTKLSFYEFMEYMKWSESEFFLLKELSLNILCKNKGYGYFQDFQNTMEYLDRFDYYKLSNYTIDKVNRYIDELLEDDLDYIRLESIPMMVSLLKRIYSSNHRQLKPSLKIFNFILNINKLSEEVINEAQNNPKYLRIQKSLVSLLSINSALFFKLSKFIYRLIHIICSMPLEKLSFEQKQEHFRNYFNPMIPFIFEYYIKCKPDLMKLKDVFGEIVEVSSYQFMLMLVQSNRFESITLNVISSLKQGETLEKNIISIFNAIVSFINCDILQEEAYLLLENHMSNFIKYLPNFYSMMLPLIYKHINSFPISLFKQLFKEKDRLVVSQFPVLFKSFSQSRYDMVNYMIISNDKVVQAHYKDYIPKLMDLIIEGSSDDAFYIMEPMGFTISVIGNSAFTKQVGDELLNHILHVQSLSLNNFDLEDIGKIIMGMMKIYKVLGKDYYRLFVVGINTFLKYLGNINVANIYTFQNLIKEYPCELIPFINEINPKIIKTFEETINVYNQELSQAIKDQDDVASNTSSVNTNSSSSSNISIESMEQTDHEITEIFTKLCDSKFFNTIFQYLINISKSSKDIYSKKMISQYVYILKEVNLNIPILHPQVVEILSDFCQHFSKVFNVIDPNDMVEILSKFQDERRHAFSIFKLAPHLETIYSTLRPIDSNANLLSFCSRFIQDTLDAKISKDKYETTNSNMNIEIKTFINLIYNIDIQVFKQISNQFILMDKFKKKSDKVVHNFIYQLIDKLNINNNKHTNEIINSILALNNKKYTMRLGRSIDVTKELESISSPCMITITPPTSKQATVNPKLPELLPSIYGNIISAILYDYSIDFKWKISTLSLVSSEFFGLLSDQLNNHFTGISEFIINRVSFHPQFCLLKGIPKFISMDKLILFPYNILSQATEYENIAGIDIKTCSQYTFNIGESKNLTQAIFRHDLKDDTHQSWELPLRILDSNPQIQDLGIVFSTIKKNYNFKHTYDDGASLPDTCLGKDLYDHLMKKKSSSNIKSLSVSYKKLNRFGKNFEGLLSLFKHLAPSTSSSKATITSSPSQAIPTISEEDYVDEKIKLNVNLSLSKKIKDSSDFNQSIIPYISFLTVYHGHNDFFFKFQSKDIYNNIKHLCIKQSGNDSHPSPMLVQFIKSSPSLTKLTFKVESIDFVTDLLNECQIEKNLNIKCIEIQIKQKLIDFVTFNNRNNQNSLLSTGGSQHIEPFNKLFNNHLEKNKTIEKVVLQYSNNQHFPIVEKSHSSKLSQKSFNIIDHPHIHILVRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTU4</accession>
    <name>A0A8J4PTU4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005410</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000215">
        <property type="protein sequence ID" value="KAF2073281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTU4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="667" mass="79149" checksum="E91523C297C26ADB" modified="2022-05-25" version="1">MIVDSYRAVFSNRYLATKIYNAVHQIQIDRSPLKYDDIVDVCWMLKYGHVGLVREKIKRNEYLYVKPKHLFSIVANTDTQMFIYLFEKNKYYALEYYDRYYQDIFLLLENPDVVRYLFQNGYARDLSHMVQPQYLDIKVLSCLLENQWIKPTPTFLVKDYNGMISTTVSTNYKKEYVDLVAKYTPSPIQHADIQVIMKHILDHEPGDFLFDALSPLFVKDPDTGYQQTLEILNKRRKQREVENTKKDYNINSTIGELLAECNMNINEECCYQLWRSCISQGDQAFFVMWKSLPSRIVVSNDAVFHLARDFTNDPYLSDYEGAHRIKEIIVESACRVGSLKILEFLYDKGLTLEVSDEEAFHRYLIKKRFSQEEPFHEGMEIEKFGRLYYSCMYGSAESFNYLYPLFGQELIEEEVLKLFETCLKSRQYHMIRVLESHDLLMADYVTEFFEFCDSHSFSHLLDQVDMVMARMAVYRDDLDDIYLELLDIAFKHNDLIAAKHIFNKHKFASLNNLTHKLDTLENLAIVDYIYKNRSQCFTEAALNNNCMQTLFDDVFNKTPVSASIIHLVEYLVQENCIDINMEKKQLFFDHDSKIEKGYKFYQVVYLIDHRNYSSIDPFIDILMNDGDWLKYLEYIYRNQDKFQNKPSFQSPYFQSNFKKLTTSPIQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU01</accession>
    <name>A0A8J4PU01_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA-dihydrouridine synthase</fullName>
            <ecNumber evidence="9">1.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_003936</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9 11">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the dus family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.-" evidence="9"/>
    <dbReference type="EMBL" id="AJWJ01000131">
        <property type="protein sequence ID" value="KAF2074748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU01"/>
    <dbReference type="OrthoDB" id="9977870at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102266">
        <property type="term" value="F:tRNA-dihydrouridine20a synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102267">
        <property type="term" value="F:tRNA-dihydrouridine20b synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000159">
        <property type="entry name" value="tRNA-dihydrouridine synthase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001269">
        <property type="entry name" value="DUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082">
        <property type="entry name" value="TRNA-DIHYDROURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082:SF31">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(20A_20B) SYNTHASE [NAD(P)+]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006621">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 9 11" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="12">
        <location>
            <begin position="30"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="32"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="237"/>
            <end position="238"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR006621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR006621-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR006621-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2074748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="316" mass="36373" checksum="F1780F833DCAAF24" modified="2022-05-25" version="1">MEDESVVKVYDSKKQSLLDRIHNGEFMKIQAPMVRFSRLPFRLLCSRWGTDITYTPMIMAAEFNRSEHARDSDFTTNDLDSPMIVQFGANDAEELSAATEKVINHCQGIDINCGCPQKWVMKEGYGANLLLHPDRIFDMVKTVKRRMNIPVSIKIRIQPDLNNTIELVKRAESMGVSWITVHGRTSSMRSSHPVDYEAIKLVKETVSCPVFANGDIFTLDQANEIKERTKVNGVMAARGLLMNPALFQGYKRTPEECVLDFIDLYSKYGDLHPVILHRHLMYMLYDYHNRNEKSEFNKIKTVTGMIDYIQDKFIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUI1</accession>
    <name>A0A8J4PUI1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_003585</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000114">
        <property type="protein sequence ID" value="KAF2075108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUI1"/>
    <dbReference type="OrthoDB" id="68611at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015743">
        <property type="term" value="P:malate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020966">
        <property type="entry name" value="ALMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052430">
        <property type="entry name" value="IVT-Associated"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47804">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47804:SF5">
        <property type="entry name" value="DUF2421 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11744">
        <property type="entry name" value="ALMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="554"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="587"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="640"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="665"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="695"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="969"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="969"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="987"/>
            <end position="997"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1028" mass="120696" checksum="C20BB4D709D190CF" modified="2022-05-25" version="1">MSSQSYFLLIKSLIRIVVAVGCALVVSYINFAIVQPHAWASIFISFTYVFLSSGFFLTSQRLPYTHGKVGYLALFCLTYFFRPENYSPVPYLKFWLTSIVPIPIIIVTGIICNIHFSSRLFLDSCTQALKTSRKLFKVINRELEYKTLEFLDFPVESTIQKYILWDTQHRILDFNDIQGKTDLTLQHEQEDELNVQQGKHHTSIPEIQIIKDIENNVKTEEQVDKQKDTETDGKEKVRKINLEKLLKSHLYFKDSLEKLNVEYSRTLNRMEVLLKETLKEFWNKDFFSFYKEISWQLEKNHRHLFTIRTAAVENLSKKSSHKIFHLLPYINTLIHQSYTIFRIMEKQLSKKYKPANFKKNHMVYLRKTKHASEEDLFDQVDLTEISTAASLESSINRLKYRQYKTLEYSQFKILICFETISKTIDEMNAKYSELHNPENYTSFDTIEEDSRVSFLLRSIKKFSKEQKLLSSTVFLLSLKLWSDKRPQKIRMIASYLNHIKLYFTEERYKRKLQKENRHSKYRKEFPDIIQPHFSLAEKAKYFYKTVILTRFLKFWRFQLQLSLGFSVFSLVYYELKIHSNFILFRNLGWAVITFTLINAPSIGAIGFLSIARLGGTIAGSFLGYASAELYSLTVDPGRDFIFLGVSMVLVFFFSMITIFQPFDKFILYFILTYIVIAYLPYPTNNPDIIISLFRTLHITFGIILVLVLSTLINPYYDHQELEKKVYTYPTSITQSFNFLVSNELIGRTNVAKKNLTSFYRASRFSAIQRNIPSFLTKAGFKFLMSEQLNTIRHQLPVQRALLFNSRFELYPFQLSKFKELQEIVKVEARLYSNLINLEFIIVERNENEKIKELSERCTPNLIKLMSEIDNGAQYLVSLMRSNSFTVDSSNNIVLQDPVVSENCSLELTNEIIKDISAYIYENRYNKDVLNKLQHISAIVFTLKSFVKTYDQLLKLLIKFSNTLVLKRVDNNNHNNDNDTKPTTTTLENDKEKEKDQDDMIFNSDDNFIEIPIPENLDPRLKVSGEPFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUV1</accession>
    <name>A0A8J4PUV1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ecotin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protease inhibitor I11 (ecotin) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000554">
        <property type="protein sequence ID" value="KAF2070031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUV1"/>
    <dbReference type="OrthoDB" id="16445at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.550">
        <property type="entry name" value="Ecotin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1230.10">
        <property type="entry name" value="Ecotin, trypsin inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027438">
        <property type="entry name" value="Ecotin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036198">
        <property type="entry name" value="Ecotin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005658">
        <property type="entry name" value="Prot_inh_ecotin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35890:SF3">
        <property type="entry name" value="ECOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03974">
        <property type="entry name" value="Ecotin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006865">
        <property type="entry name" value="Prot_inh_ecotin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49772">
        <property type="entry name" value="Ecotin, trypsin inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="15571" checksum="60FD71B5098833F1" modified="2022-05-25" version="1">MSVESITPFPAAEPGFKRFVIYVPELQDEFSAKVELIAGKMMLVDPNTRSLGGKFEEKDLEGWGYTYYVLPAVHGPMATRMFFPGGDSAKENRFVSIIPQDLIRYNSKLPIVVYVPEDITVKYRVWNASESSTEAQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PV19</accession>
    <name>A0A8J4PV19_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_004500</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000160">
        <property type="protein sequence ID" value="KAF2074182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PV19"/>
    <dbReference type="OrthoDB" id="17803at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF26">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DDB_G0283821-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="185"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="6">
        <location>
            <begin position="924"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="39"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="111"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="746"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="882"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="589"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="746"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="799"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="889"/>
            <end position="901"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2074182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1108" mass="123795" checksum="189C25A4E19DB250" modified="2022-05-25" version="1">MEQVESNNEQTVTSGHHDDLDDLQEQVREFLITSGEDALNELYSSGGSGGTSNSNNNNTTTTTNTTGGTEQSEEFISSEVAQIASKFQENDGTVRSSKKNRRKFAMNILNNSDDISSGSLDSVASSSSTSSTTNTKPPTTTTYSPPPSRPSNNNPIHSYHTQNVSPYKPPTSSNLDSSPSTLGKYQLVHHIGTSYVGNTWSSVPPNTSEHVSIKVINRDRINLVFQNIILNDFQVLKSIKHQGVVNYLTVIHDHTGLGIVQEFYENGSLYDWKARIAGGPIQEGTIIKYISQILESLIHLHANGVIHKNLKSSNILLAKGGKCKLSDFGIGCTVPNDTSIRYSIIGHPNWQAPEILSMKPYDERVDSWGIGCLVYELITGSPPLSNLDPIQALVEIIKTSSSNDDESLFSQLNSSLEISKELEDFLSLCFIKNPNRRPTCTELLKHAWLVKDESKLTSFQHIGHLSQTINRSEIFSLDFLSQSERNEKEISDLAIGMPTEIDFKKYDFQHYNAEQLDSLATDEQKMEQLKSIINHNSTIGNNLKYSMQDLLQEQAKCYDIYFSMKTKMQEIFNQNKTNAKLSAHSNMLLKRTNQMASELVKKNETLQSNIKRLEEHFITKDDCAKKLANVVYRNKTSFDSLLNPTLAAPLTYQTGSKSWKKGQDKRAFATLKDNFLFLFKNEKASYPIDVIYLNDKKNISFSTTNNDSKKKIYIISIGTIISSQELLNLDENGNVCNLNNNNVNSSSNNGSGNGSNSNNSSSNININNNSSSTISTSSSLPSGLSNYSSSPPSSTNSLGKEKEKDREKEKDKEKDKEKDKDKDLLNATSASSEPTLFWCHLTFDNQKSLESWMGVLESVVPWYDKKFYEISKPIVEKKHTKQKSLDSISNKKDDVNWRKESGTAGAGASGGNSNGAKFQGVIGVRIEDLMMRENPNVEIPYFLTKMLKYLEQHLDEEGILRLSGSSVEIQDIKNSLQRGDSIDYTNKDTHAVTGILKLFLRELPEPILSDALRHQATEILSSNNKSLSDKEKLKEILYVLGHLSKPTYNLLKHMILFAKRVVENSDVNKMVLANVTTCFSPTLRLPPGLFSFLINNYDSCFPHYTMSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVV1</accession>
    <name>A0A8J4PVV1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000137">
        <property type="protein sequence ID" value="KAF2074635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVV1"/>
    <dbReference type="OrthoDB" id="19359at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14596">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14596:SF72">
        <property type="entry name" value="ZINC FINGER PROTEIN MSN2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="680"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="917"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1088"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="917"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1088"/>
            <end position="1126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1183" mass="132433" checksum="22F4D74F42283070" modified="2022-05-25" version="1">MQPQFQFGTFDARKYGYIRGRSILNSLINNQELEEWGVTDVQVISGHVALRFQQSRSICLFDATTSQFQLSDSVSNSLESQLLTKKFLDSPLSPSTQSLPILACSFPTNIIKFGFEKYIISSSSTNNNNNSNNSSSSSKSMINNNSTANTLMVESNPLRFYVFESDGTFHLWEWNTSEYKWKYTIKLFLPLVNRNIDHSSSSSGFGSNGSGNTSPIMSAVEANVRITNAFVFGSSQGISLAWIQVPNTTPPPLNLNSNNSNTTTPTTTTTTTTIYNRVLNFDQYVKKKPDLVSKQIIVRDSTSVKVGTGSNTTIDELYPTNLNITGLESYELKKEWSDEINGQENVRVLSTKLGIWFITGSFVLLWLLRNKMFNITFIKNSQHHQQSKDSLSLPTPQSCDIHGLISPPMNGNHHSINNEEKKQQPTPSLAKYIKDVLTVCKNPANQDLLILEPSGRLFSCEPSNEKEKLTVKLLAILELPSDKEQDVSMVMSQSALMFVDSKNAWFYDTKCGKLLSKTSLPLVMKKGGLLWQNGTAGVLWGCGVWAAGQGIYELRSLTPVTATIQSMASHKIQQQPTAAGGAGLHQHHGKTNASIGCAPTTCKQFDFRLMESKYLLDMALQEKDEQTKIEICKVLLPKLENPSLVIAILSDLESFQSCKFIFDQLGDFLEQYDYYEQLKKKQQPGGATTGGSATDEDEKSKNHKIRNSFFYHTPLNIKMIPMLKEYYRMQKLATAPITRLAATPANSISKCELDTQDWGLLGPLEQVDDPSILSVSHETIQVLVSKYPALLLQKLEWVLQIPTQEYTRFSDIYADPLSLPSPIINPLLFHSEESIYQAKGKEHLLAKTTSSSTPTLSSPSLLSAMMSDKPIQANYPIFETLCNLYYSTKPEHLVPFVRMIHHASLIKSLESDSSDVNSSYLSLNTNNNNNNSNNNNNNNNSNNNTNNSNNNNNTNNNSNNNNTNINNKIPEKDHFMRALLSIPILPPSPNQELQIDARFKLLIAIGHKPNALRFLLNIGKWDKALEMMKSVPYGDNETFVLYEILLSFCIERKDSVKLKQCWDIIPKNFSVFNLLSLLKPFSNTLKNNNNNNSLNQNNSNNNNNNNNNSSSGSRINTSNSINNNNNPPIYNPIVLSTPNNSDGLTIDMFKGQILKMLNLDTPKRNHSTFYSMKSINTKEINIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW84</accession>
    <name>A0A8J4PW84_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Band 7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003827</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000125">
        <property type="protein sequence ID" value="KAF2074872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW84"/>
    <dbReference type="OrthoDB" id="2105077at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000004">
        <property type="entry name" value="Putative membrane protease family, stomatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2090">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043202">
        <property type="entry name" value="Band-7_stomatin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264:SF19">
        <property type="entry name" value="AT06885P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264">
        <property type="entry name" value="BAND 7 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Band 7" evidence="2">
        <location>
            <begin position="68"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="317" mass="35478" checksum="21BE63D4AE37ABDE" modified="2022-05-25" version="1">MSLLTQSTPLFLKSASAALLNQSSVVVPLVLASAKRSFTKTSSIHNISNSNTNKLFLNSNSNKEAKRGFFTIINQYEMGVTFTLGKVTSVKKPGIRLAIPMLQDIVIVDTRTVSFSLEKQEIISKDNISLIADAIVYYRVIDPQKAVCEVADHDTVIRELAQIKIRDILSQNTLDDVLHNRDKFSDEIYEDVKSSAESWGIEIQRINLKDIKFEEGMARAFAKKAEAERLREATIINAEAEVQVSKKILEAAQTLESSPVAVRLRELDALVQISKEPSKSFIFVPTGLFNNNNNNVAHLQGMMDMFKNDNKDNNKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWB3</accession>
    <name>A0A8J4PWB3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007963</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000454">
        <property type="protein sequence ID" value="KAF2070721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWB3"/>
    <dbReference type="OrthoDB" id="24203at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="543" mass="61608" checksum="C41384ABFD5AE980" modified="2022-05-25" version="1">MFNIIVELYCNTLIKDLTKNINIQQITFDYQCSFKEGDLPQGIVDLEFLTGVYPISSGLFPNTLKQLYLGEDSGPEITKQMLPLGLTNLLVDNNIVLDITSLNHLTFLKSLFLYSFENPLDTPLPKSLEDLTLSNFNQPIHKGVLPLGLKTFMMGDDFDQDLYPGMFPDTLTELGLSGQLDFETHKDQIHQILPKSLTKLKCLNYMSPFYVGFLPESLERITMNSQHENKVFPASLPNNLKTLIFGKEYKTFTLQRDVIPPSVTYLSLPVVFDQEFIDQDGFNILPPNLTYLAIGGDYRRKFIKNSLPNSLKTLWLTTYKLSFEPNVLPNSIHTLFFDCSSLETLKPNVIPPSVTKLIFLRGFNAYIEPNALPNSITQIVFRERYRHVILPGVLPNSLSSLQLSVFDLTDVFLPNNITHLTIELHKDQCLFANFIPLSVKKLLISGSSLRDSLKKEFFPPNLTFLKVAPIHFPLEPGMFPPNLHTLDLGSYFNQSININSIPPSVKYLCLPKTYDLKKANFQSTTIPILGTSLSYKENNNNNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWM5</accession>
    <name>A0A8J4PWM5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6748 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004167</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000142">
        <property type="protein sequence ID" value="KAF2074540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWM5"/>
    <dbReference type="OrthoDB" id="21881at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040405">
        <property type="entry name" value="DDB_G0275255-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046636">
        <property type="entry name" value="DUF6748"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34411">
        <property type="entry name" value="DUF6748 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20533">
        <property type="entry name" value="DUF6748"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035264210" description="DUF6748 domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6748" evidence="2">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="283" mass="32131" checksum="0F14143BA6FAF941" modified="2022-05-25" version="1">MYIHKSFILFLLGLIVFSAVFSANTDTNTDTDTDTDVESDNERSGLGRVRYFQATKDLRKCAFPQCGGYFLKMVNIGGQQNDIYVSSIEGAKIDDGSLNEFILSGRLSNGPSFTRTLYIQDTYRLLPLDSPSTLEIKRYFIYRNNVAYELNTGRNYRVNYYVENYSNQINNINEEWLDYKINGHSVIYGNVRNGILDIDRIFIQLPDPQYCPDFPVAQCSPGYIATYRVDNTRCILPTGCARQGICTLSIPTCPNGYTLMSVNSKPNGCPKYYCDPSFLLIFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWR4</accession>
    <name>A0A8J4PWR4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_004535</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transcription factor STAT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000162">
        <property type="protein sequence ID" value="KAF2074164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWR4"/>
    <dbReference type="OrthoDB" id="19300at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09919">
        <property type="entry name" value="SH2_STAT_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.340">
        <property type="entry name" value="Rel homology domain (RHD), DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041604">
        <property type="entry name" value="EF-hand_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037059">
        <property type="entry name" value="RHD_DNA_bind_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001217">
        <property type="entry name" value="STAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041410">
        <property type="entry name" value="STATa_Ig"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11801">
        <property type="entry name" value="SIGNAL TRANSDUCER AND ACTIVATOR OF TRANSCRIPTION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17901">
        <property type="entry name" value="EF-hand_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18214">
        <property type="entry name" value="STATa_Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="5">
        <location>
            <begin position="688"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="31"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="94"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="145"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="243"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="175"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="191"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="243"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2074164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="811" mass="92232" checksum="C95DCDCB2CB70746" modified="2022-05-25" version="1">MDNNNNNNNNKDSITTVHKDWMINSTDMMNNVFSHNRTSNDEGNHHSNNMNNNNNSGGSGLFKQDSTLFFDEFKQQTNDSSSLSRGDSFGFMLRSNTTGLGNNSSSNSNNNNNNNNNSNNNNNNEGSTIPLSLTSQNSLFSSLFSNQQQSISGTSSTPPASLNPYSNFQPNLVINNTPTATTTTTTANNNDYDQNMDSSNPPDYNANTYDDGEEPSTPPTPPVSAIEWNLSFIAKSNYDYQQYNNNNNNINNNNNNIQQYNQHPQQQEIYQQQQQSSLTKNDPIDPSEYESPSKFQRTDTFTDETFTRLRADPHFRLSFLEEATSQLTRSSSSQSQELNRNRSFIMTHYETGCIPISHEFYNNLLLRYNSRPELFKEAKEKQSLFVKNLSNLELGHELHTLYSTIKKDIEEENSELKHLLSLRILEPLDLRKIREALEGLKSHLRICDVLHQELKFLLSKKKSECCAALVITLQPYSQVIFRGKGIPESYKVELVTGVLPAERVTTVLATINKADLGKKKDKILSGDGHLENDQASFQSPQMEAEFKNLKVNVSTRMLPSSLKFVSTVIEKGKEKTIESVPSSPMVVITNESQWAEAAGKLLTCDAFMDKEEITWEYFANILHSHILTGTNQTGEIKRKLHSWEFEYIQKNYFNSKTTVSKLESTTFWAKFGPILQTVHFKRHIAPLWYSGCIYGFITKNECNSYLMSLPEGSFLVRFSDSVPGSFAVAYVTNDDNERVKHYLVKPEDIGANKTLPDFLREKYQFKVLYQVDPSKRSLHPKDKDEILDPYYSKRIKNREQQTGNPGYVSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX25</accession>
    <name>A0A8J4PX25_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GPN-loop GTPase</fullName>
            <ecNumber evidence="9">3.6.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_007576</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Small GTPase required for proper nuclear import of RNA polymerase II (RNAPII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Binds to RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="9"/>
    <dbReference type="EMBL" id="AJWJ01000412">
        <property type="protein sequence ID" value="KAF2071111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX25"/>
    <dbReference type="OrthoDB" id="243313at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17870">
        <property type="entry name" value="GPN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000888">
        <property type="entry name" value="GPN-loop GTPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030230">
        <property type="entry name" value="Gpn1/Npa3/XAB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF8">
        <property type="entry name" value="GPN-LOOP GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="317"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="271"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="317"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="348"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2071111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="387" mass="44174" checksum="CAFCCDF96B0C4CE6" modified="2022-05-25" version="1">MTTTNTANTTPSVEGEKPQQEKKPINMIILGMAGSGKTTLLQRLRAHIFEKKIPGYIINLDPAVAKLPYTPNIDIRDTVNYKEVMKQYNLGPNGGIVTSLNLFSTKFDKVLEIVEKRASQLKYIILDTPGQIEVFTWSASGSIITELMASSFPTVLVYVVDTPRTTDPTTFMSNMLYACSIMYKSKLPMVVAFNKTDVTNHRFAEEWMSDFDSFQDALTSDPSYMGNLTRSLSLVLEEFYSTLQSVGVSAVDGSGIDEFFEKIELAGQEYYKYYKADLDNIKKEKAEKEQEKANKEWEKLRRDLEESKGGKVVYDFKKDGKSKKSSEPQPRGVYDDDEDDYNTNNRDDQEEDEEEEGIYQYASEKEREADKLEYESLMRSMKDQNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXI7</accession>
    <name>A0A8J4PXI7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">LisH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000093">
        <property type="protein sequence ID" value="KAF2075591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXI7"/>
    <dbReference type="OrthoDB" id="21591at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="437"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="527"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="618"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="142"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="219"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="442"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="527"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="565"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="577"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="658" mass="73651" checksum="1498C99C1BCF06F2" modified="2022-05-25" version="1">METQNKLPPHHLIRVSEICLLVLTFLKDQNFKNSYETFLKESASLIKGLNKTPARTVKSLSFILNEYIVLKEEKKKKDQFVGCLLSNKLSSQDEYYEKIKGSLNMLYNMVDDYTSFRSKSISDISYSSYAFSDVVDYNNNNNNDFNNSGTQKNNSSSSNNNNNNSSSNTSASTNQLSQHLYSNNNLKRKKPDTPPANNSPNMVYSTRLSSGMNTNPLYSTPQLLSNPTTTTTTTTTTSAPSSIPPTIDLTMESPITKHTASSSNSSSVSARRKNMTPKKLSISPNGANNSNPLQNVQYQNTHHQQQHHHQQLLQQPSTQLYNQTPIQQPQQNIIQPQKQASPMSEIEELLNNPQFSEKIADHINHYLSQQNIGWPSSSTNNSNQLLDNIDQQQQQQPSHQESNYIASANNDEYNQTNNNYLYSNSHLQYQTTQQPMMEDDINNNNNNNSNNNNDIYNNQYNTQNNDLVDNIVMSIESDPSIIHMINPMFGSDLEHQINAATDSNGFYSEPLIPFDNDQHQHFNQTHNSNSINIPLTPGTTRRTASSTNFTSPPRYKSSKTLLFGTPSTSPNAPMNPNSATLTSTTITTTTPPPIVNNNNNNNNNILKTSDLTTITPVAPPPPPPMNSKSKSPLAPVHNNNNLIPDDVEQFLSSLQYDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXT8</accession>
    <name>A0A8J4PXT8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003028</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000090">
        <property type="protein sequence ID" value="KAF2075655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXT8"/>
    <dbReference type="OrthoDB" id="10066480at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007972">
        <property type="entry name" value="Mtfr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14215">
        <property type="entry name" value="PROTEIN OF UNKNOWN FUNCTION DUF729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14215:SF0">
        <property type="entry name" value="WH2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="260"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="120"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="341" mass="37080" checksum="7C3071BFFAF93CC3" modified="2022-05-25" version="1">MSNNYSSETTTGSNRPMTTYYSSESLGIQLLKITFQIFELAGQSTKTTYSRLFYDLPEYLQREIKSKNGFYRKLINIIFPSTSANSNSIYIKSVSLQQQQQQQEPVTIDTPLNSTFVTSPISTASSSSSPMYNVSSTTSTSTSTSSPSLNNTTININTAGGAPVQSQEKINSLESELTRLREEFAKIMAAKNAANSSTASTTTPPPSSSVPPPPPPPPPLAQTKKLEIKKSINNSANTNNNSTPMKQPILSMNDILKGGKSLKRSDVVRSPGGTPLKDTTTSSNPVVSHQDFIANALKKKFQNTYSSPDHKSAANHDDDDSFSFDSDDDTSKTPKKSLFVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXU7</accession>
    <name>A0A8J4PXU7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003865</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000127">
        <property type="protein sequence ID" value="KAF2074839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXU7"/>
    <dbReference type="OrthoDB" id="19340at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="137" mass="15786" checksum="5F28D1259145267C" modified="2022-05-25" version="1">MDTSTNTNTSTSTEQHPVDAQEQEKRDKIQQYLESLKFPFEYKWDLEYYEILLKGAAQNPVLPLVIIRVDKMLSTEERSLVPSHCGDIKITMEYVMTLDPNDADVQKEMKEMQDAENEGDEDSDESDDGDDISMKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXZ8</accession>
    <name>A0A8J4PXZ8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Purple acid phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_003236</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.2" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000099">
        <property type="protein sequence ID" value="KAF2075455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXZ8"/>
    <dbReference type="OrthoDB" id="45007at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00839">
        <property type="entry name" value="MPP_PAPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.380">
        <property type="entry name" value="Purple acid phosphatase-like, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041792">
        <property type="entry name" value="MPP_PAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025733">
        <property type="entry name" value="PAPs_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015914">
        <property type="entry name" value="PAPs_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008963">
        <property type="entry name" value="Purple_acid_Pase-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45867">
        <property type="entry name" value="PURPLE ACID PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45867:SF10">
        <property type="entry name" value="PURPLE ACID PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14008">
        <property type="entry name" value="Metallophos_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16656">
        <property type="entry name" value="Pur_ac_phosph_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49363">
        <property type="entry name" value="Purple acid phosphatase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035338498" description="Purple acid phosphatase" evidence="3">
        <location>
            <begin position="19"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="Purple acid phosphatase N-terminal" evidence="6">
        <location>
            <begin position="23"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="4">
        <location>
            <begin position="137"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Purple acid phosphatase C-terminal" evidence="5">
        <location>
            <begin position="359"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="429" mass="49004" checksum="882CD4115A809B77" modified="2022-05-25" version="1">MNKYIGALFIILISVCVAVDLTPYGIKLALTKEPQNIKVTWLTNDKGDTPTVLYSRASFTPDESSSNEFYPIHSNAGAVESFPSLVGYWNGNANQAVMANLTAYTTYFYYVGDREANQWSQMFNFSTRGFGRSDYASIAVYGDMGHGGVGIDRDENYTLSHIMERQGYFDFVLHLGDIAYADITKGSHIFGNQTVWDEFLGVIQPVATILPYMVCPGNHDVFFNLDVYRKTFYMPTDKEDDSWYSFDYAGAHFVGFSSEHDYIPLSTQYNWLENNLKHYREKNPNGWLIVYSHRPFYCSSKYQWCEDFDVFKLMFVSSLEKLLMKYNVDIYLAGHSHTYERTLPVYDNKVLGTFQNPKATVHITIGSGGNKEGPTYNWLEAPEWSNGLRSSHTGFGKFTILNSTTIQWDFVDNYSDSIVDSFVVTKGYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PY57</accession>
    <name>A0A8J4PY57_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003988</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000133">
        <property type="protein sequence ID" value="KAF2074712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PY57"/>
    <dbReference type="OrthoDB" id="2317183at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011697">
        <property type="entry name" value="Peptidase_C26"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07722">
        <property type="entry name" value="Peptidase_C26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="555" mass="63631" checksum="C4329A3E13678D9C" modified="2022-05-25" version="1">MNRDPTMQDVVDYFQAAIANGRSQAQLANDFRVHKNTISRIINRIIKKSHKVLKQFPRVNDPQQNQALDEQIPDLVNDEEPGPNPVEKDEQVALLKNEEQLPRPAEEGEQVPDLMEHMKIIHPPAPTTLGEEEKQQEFAPLVIGQPVTTPSFGQGHSFILEQEREILEQISKLENQLLKQKELTPNQGEESVIVGLPYRGPKGTIFDSLAFQSNQMVTIPLCKEIDSTYNQRRINEELRVLTYNLSKKIEIQSNYVSPYVDIIVIPGRSRKFEHEQKRANTDGNLISQALKLGQPILGICAGEWRLFAHLCRRDLTLKFWEKIADMKIKMLKNAQETLNFFKKCEDDIEKSLYNLDTLDGNLKYCELWIEKINNQLQGLVLDDDVKILKSLFEKIMQDDTEFLKDTQNHNSRRMMSISLVQNKVNYNNMVHSLELKDSLVKQFMEKKDTVEIFEEKSVNSVHWRAINEKHLPNGVEIGAISPHDGVPEAFSSTLGSPLMGVQWHPEAYNANHPHQAPHRRMIQRMGVCGLAYRARCKALQLLKTKQLSGLLNSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYV8</accession>
    <name>A0A8J4PYV8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nascent polypeptide-associated complex subunit alpha-like UBA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000965</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000021">
        <property type="protein sequence ID" value="KAF2077718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYV8"/>
    <dbReference type="OrthoDB" id="285219at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14361">
        <property type="entry name" value="UBA_HYPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038922">
        <property type="entry name" value="HYPK_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044034">
        <property type="entry name" value="NAC-like_UBA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19026">
        <property type="entry name" value="UBA_HYPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nascent polypeptide-associated complex subunit alpha-like UBA" evidence="1">
        <location>
            <begin position="31"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="112" mass="13043" checksum="8C2CF116076696A6" modified="2022-05-25" version="1">MEDKRGTNAQDDELHSMNISLENLVLEEKEIKKSDIEFISYQMNVSIECATSVLRECKGDVVQALCTLVHLCKEYELYDIESPVKKKQMVPQQESYQDGMFIKRLVYFNLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ17</accession>
    <name>A0A8J4PZ17_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sulfite oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_001885</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-molybdopterin</name>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000048">
        <property type="protein sequence ID" value="KAF2076808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ17"/>
    <dbReference type="OrthoDB" id="10051395at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008482">
        <property type="term" value="F:sulfite oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006790">
        <property type="term" value="P:sulfur compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.420.10:FF:000002">
        <property type="entry name" value="sulfite oxidase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.420.10">
        <property type="entry name" value="Oxidoreductase, molybdopterin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005066">
        <property type="entry name" value="MoCF_OxRdtse_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008335">
        <property type="entry name" value="Mopterin_OxRdtase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000572">
        <property type="entry name" value="OxRdtase_Mopterin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036374">
        <property type="entry name" value="OxRdtase_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19372:SF7">
        <property type="entry name" value="SULFITE OXIDASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19372">
        <property type="entry name" value="SULFITE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03404">
        <property type="entry name" value="Mo-co_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00174">
        <property type="entry name" value="Oxidored_molyb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00407">
        <property type="entry name" value="EUMOPTERIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56524">
        <property type="entry name" value="Oxidoreductase molybdopterin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidoreductase molybdopterin-binding" evidence="5">
        <location>
            <begin position="54"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Moybdenum cofactor oxidoreductase dimerisation" evidence="6">
        <location>
            <begin position="262"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2076808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="398" mass="45397" checksum="F88E530024E9E343" modified="2022-05-25" version="1">MSEHKNDKEAYKNEPYRNQEFVVNFKDPFNAEPTPTRLIQSYITPTEHFYVRNHAPVPDIKESAYFLRIEGLIGKPMVLTMDDIKSKFEKVTIAATLQCAGNRRTMMSEYKKVKGVGWGIASLSNGTWSGCRLRDVLEYCKAFSKDSGAFHACFWGLDFGCPEEPDGKPYGSSITMEKAMDPEGDVLLAYEMNGETLTRDHGYPLRVVAPGIIGARSVKWLGRIIISPYESESFFQRRDYKIFHSGIDWNNITDKVWDSTPSLQELSIQSAICSPAPNSTVYLPFTIMGYATGGGGRKIERVDISLDGGESWDYAEIMTEEKGPVNRYWSWVFFRYTITHLNQLDHQKQVPLKLMCRAWDNASNTQPRDVKDIWNLRGVMNNCYHIVDIVVENKKSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ43</accession>
    <name>A0A8J4PZ43_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000017">
        <property type="protein sequence ID" value="KAF2077868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ43"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38736:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38736">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="28066" checksum="0D18DC9B44D71D92" modified="2022-05-25" version="1">MVSLIWKFNLLITIIIISLSITSFFLPWYHINTHSSKGPNTKHPVHREYKFYFDKWEFYSNLTNDGNLIVENYHSMQEVQTTMFICFFFLLIGTLFTFTNAIFIIFGVNEKSKTNFFVNVTMIVLSVSASVFYLISLCVNTRLIKDFRSSAHLENVCINKGDYIYQCKSFIGKISSHNYSHYSWSPDSGFGNIVVCLILGIVSFGLLILVTKRNSKKDWEEIKEDLRAQNNNNIQQIAEILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q029</accession>
    <name>A0A8J4Q029_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Actin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003228</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Multiple isoforms are involved in various cellular functions such as cytoskeleton structure, cell mobility, chromosome movement and muscle contraction.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000098">
        <property type="protein sequence ID" value="KAF2075492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q029"/>
    <dbReference type="OrthoDB" id="27221at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000007">
        <property type="entry name" value="Actin like 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000050">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00432">
        <property type="entry name" value="ACTINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="347" mass="38952" checksum="19E54FC2A6FBF5B5" modified="2022-05-25" version="1">MEDPLAIIIDNGSSYCKAGFGGDDQPRCVVQCTVGVSDKKYPIESGIIKDWDSMERVWFHVFYNELRVAPEEHPVLLTEAPLNPKANRERMMKIMFESFAVPALYIVNQAVLSLYSCGRTVGVVIDTGEGVTHSVPIYQGHALQHAITRLDLAGGGLTDYFIKLLNERGYSFSNYPQTNARELVKDIKEKLCYVALDFEAERTIFTTKSYELPNGATINMGEERYMCPEVLFQPSIIGLESNGIHEITFNSIMKCDQDIRNQMFSNIVLGGGSTLFPGFEQRLKKELMALTPSSINNSIKIVKSHEPVHCVWIGGSILSSIASFQTCWISKQEYDEIGTHIVHRKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1P6</accession>
    <name>A0A8J4Q1P6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002174</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000058">
        <property type="protein sequence ID" value="KAF2076497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1P6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="851" mass="100110" checksum="86CFFF2781F0186C" modified="2022-05-25" version="1">MSDEVFETEPYQCLQSLDDLLMSINPVKDNQGYFILDNKYKTLNSIYLNVDNNNNDNKQLFPLNNNNINTLLNTGNAKEIKYNGKTAMKVELYRMEENSKYTNALNRFLYSSCYHMNIKYIRNRTKPQLRGVFIYSNDFFIEPYQSHFDSNSNHVMTIVVILPTDHNGGEMEFVENKGKTVKEQIDNCKSQIKFIAFKGVNTLKQCYSITNGKRVCLIYDIFNENNTTPPKEPPQEIVSSIVNKYLNLWFHEKMEIKITRDAYKIELTQLSNDLLKEFNIIKNNNNSDISHSFIDNRPCRLKNNNRFRMTPITYNNNTFGKNLKRAPDIDNHQQEKRLKVGNSKNMEKEAQNILDKDEFDSYYHFKEQDIESVGKVIIEALNKFSCTKFDLEILEGTIEIQRTTFNHESDIFYSCKNLKSLNSCSKTNQVHDITTIDCQCILPFGYSASCDDKHSESEFEDKNNTKTYYCTWEKRALFIQHREWRGSTNPVQYIEEKICKWRTNNISCDSERVQSVTTRVFNVLLSPSSKRVLPDNVIHFIKQMKESKVHLIRYYTFKSNKDLTFAASQFGPMAEYLLEQERNALILKLLTYQLWQFSILIHSYLTYSFAGHFYESVVKFYQQHPHRFTNDNWSKICQLIAPKFNDQGVQIMKKYYTTLSLCIPLLNSPLVKLKDLLTQGYPTNQLYQEYLKDLLQYKAPQSMIDQAPKLLNHCVEQMYFKPLRGITLAEAFGKLRLFDQHFNFMNRFLRYITSTYSWREWYSLYEDIHLNMDTRSLSVAHFHHDLLRHILNFYSDNILLTNYTLPPIAKCVPLAPLLVPMSMLVKLILSNPYSKAAKIPYLEYTENGYGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1Z4</accession>
    <name>A0A8J4Q1Z4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein bL32m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_000916</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bL32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000019">
        <property type="protein sequence ID" value="KAF2077795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1Z4"/>
    <dbReference type="OrthoDB" id="2014905at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051991">
        <property type="entry name" value="Mitoribosomal_protein_bL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002677">
        <property type="entry name" value="Ribosomal_bL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01031">
        <property type="entry name" value="rpmF_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21026">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21026:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL32M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01783">
        <property type="entry name" value="Ribosomal_L32p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2077795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="114" mass="13253" checksum="65829482E0E0D863" modified="2022-05-25" version="1">MNRLTLLGSVAQKYRFVTPVEQVIVGGRLFNAPNRYINNQIQHQEQAQPELPIEAEQGEIELTAVPKKRVSYTRNRKRNSSKKYENIHNYTVCQKCHSPVLMHCVCMVCIRKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2L2</accession>
    <name>A0A8J4Q2L2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005679</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000233">
        <property type="protein sequence ID" value="KAF2073001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2L2"/>
    <dbReference type="OrthoDB" id="17470at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF122">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN SKYWALKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="1">
        <location>
            <begin position="10"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="180" mass="20847" checksum="9A30451FA1D73741" modified="2022-05-25" version="1">MNSLLPISSKIFNLENFQDSIMPNWFSRKHELSLLFRASEHDFRASSFHSHCDAKGATITLIQTTNGDIFGGFNSQSWNSKGYNYGDIGCFIFYLKKDEKINPIRYTPVPDPDDGKYCFVFGDSHFGPVFALDIFINDQCNQNDNIVYQPFHYYSNNNTKHKNSPAIYFKVKEYEVFQVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q354</accession>
    <name>A0A8J4Q354_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_000780</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000016">
        <property type="protein sequence ID" value="KAF2077902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5951731at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052014">
        <property type="entry name" value="Dictyostelium_Tiger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31341">
        <property type="entry name" value="IPT/TIG DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035309061" description="EGF-like domain-containing protein" evidence="5">
        <location>
            <begin position="21"/>
            <end position="2667"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2627"/>
            <end position="2648"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="2342"/>
            <end position="2380"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="2370"/>
            <end position="2379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2077902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="2667" mass="283636" checksum="BED264C096E330CA" modified="2022-05-25" version="1">MNISLFVYFLISLCILCAAAAPQINRVSPSFGGFSTQIWIEGTGFGATAASNSVSGNGYVCAIGSANITHIRCTFQSASVTGIPVSLSIGVTTGGIQSNTLPFGMISISKFLQSNLIFWIQGGFSSIPVASQNKIILRTQDQTITTPVRYSETGLQWDVTEQMVRKGGLVEIFDGATNTVVTQTSLQYTAIVQEVKYYESTISIKGFLYSASTTVTTSQIYNVVDDCPVSSWTENTLVCTPVSTYYIDKYYFPYTIKQSNNLVYSSGASLPMIQSFQNTGADSSTLKIDNLPQTTPLRVLGLTATPIVPTAISTTGVVLTYPPNAQCGNAFVSSDASLNFERFSTSTLVCPIPLIQNVVTKPDSSNNYVLTFNGKFLNNQQYKIGTEILFYSIQYSGGANQSCPTLTTSSNANFVYTASCQVPAIAIPTTFKLYASTLPGQSSSILVGYQPTIETASKTNYKTTGTVTLTGKAFSSFDLVVTIGGSPCRNPVVASDGLQLTCTFDGDAVVVDFNKPLEVVVSIGSTYIDKKSIFYYNRPAPTISSSTTTNYGIPGTVTITGTYFYSTNLVVTIGGSPCTSPLSAADGNSVTCQFDSSVTVASIRNPLEISITIDSTYTTKRSVFYYTLGVPTINSCTSTDYLVPGKVTITGAGFAMFSLVVQIGGRACTSPSVGSGGTTIVCDFPANVVVTNFKAPLTVSVNMDSTVTGTKDVFLYNQPQPVITSASSTSYGVAGTVTIIGQYLYTTQPMTVTIGGVACANPSVNVQGTQTTCSFSSTANPIDFITPLPVFVGFESTFSATKNVFLYKRPNPVLTSASSTTFDTPGKVTIIGLYFSFSTSINTAVNIGTAACTSPIITGDTQIVCDFASKVAVPDYNTLLDVKVTLDTTYTATKAMFKYIRPMPSLTSASSTTYGTPGPITIVGKYFSPSSTGGIVVTIGGVGCTLPQRTGDTQIVCSFDSTSLVVTDYTTVFDVRVTIDTDYVASKPLFKYLRPNPLLISASSTIYDTPGPVTIVGKYFSPSTTISVTIGGQSCTSPVATGDTQIVCTFQSRLAVTDYNTLYIVGVTISGSFSSSKPMFQYIRPNPSLTAASSTTYGTAGLVLIEGKYFSPSTSDSITITIGGVPCTTPQRTGDAQIICSFSSLAPPGDYKTPLPVSVTIDTASTTSNLFSYIRPNPVLTSATSTVFATPGPVTIIGNYFSPQNNMVVTIGNAPCSSPVVTGDTQIVCNFASTAPVPNYQTPLSVSVSIDNTYSVAKQVFYYIRPTPTIISASSTNYLVPGIVTITGTFFAPDSLIVTIGGSSCNLPTVAQGGNELTCQFQSNVPVTDFKSPLTVFVSVDTSFIASKAVFYYIRPNPIISSATPTTYDTAGIVTITGSYFASSDLIVKIGNAPCASPTVSQDTNQITCQFDSKVAVTDYKVPLDVFVSIETTFTATKAVFLYNRPNPILTSTSKTTYGTPGQVSIVGKYFSPQDALVVKIGGVTCTSPSVTSDTQIICNFASSAPVSDYAVPLEVFVSLASTNTASKSIFYYNRPQPIISSASSLVFNSAGKVTISGSYFAPPKNLVVTIGGTQCTSPSVSSDGTSIVCSFSADVTVSDFRDPLPVTVVIDTTFTDTKSVFYYNRPSPTITAATSTKYDVPGLVTLTGTYFASTNLIVKIGGGPCTGPVVNSDTEIVCSFESNVVVSDFKDSLVVSVSVDSVSGSAAVFTYIRPDPIITSATSLVYKTPGQVTISGTYFAIDNLIVKIGGGACSSPVVNSGGNQITCQFNADVVVVDFETKLTVFVSVDSYSSSNSVFSYTRPNPVIISSTSTVYGVPGMVTITGTYFDEDDLIVKIGSVECTSPVVSQNKDIIQCKYPSSEPVDSKFKNPLSVYVSVKSIYTSSKPVFYYIRPLPVITESSSTVYATPGQVTITGNYFASDDVYVEIGGQECTTPVSTANVITCDFSSNISVDRGFSQKLNVLVSIDTDFNTTRPVFTYLRPNPVVTSSTSTLFSIPGVISIAGNYFDTKDLIVEIGGSSCVSLNVNQDGTLVTCTYASDIDVKNYDLPLQVFVSVDTNSASAPVFFYTKPTPTITEASSTIFNVPGQVTIIGDQFVNDDLAIEIGGANCTSPNIVTPQKVICDFPSTAPFVRFADPLDVLVSIKSIYTATESVFYYLRPNPTIKSCSSLYFNIPGQVTITGTYFASTNLVVIIGDEPCTNPIASEDETTITCLFESNALFKDYHTPLNVTVAVASKSTTSALFYYTRPNPVIISATSTKYGVPGKVTIKGSLFESQESQQLLVSIGGSTCTQPLASQDGTTITCDFASDVPDNHQALIVSVSIESKYKSAKAVFLYVSPDKTCPIGIGDQVCSGHGTCNQQQFTCECNSGWESSDCSLPNKGGNIPDPSVHPNDTSSTIITPGGTMFDVGIVLVNEMDTNNNIIQSYNISNISWNNITKQDSVYSYTTTLPNTKSTLNVQLTVNSLDQNVYVDFAGDVIPILPKSIKYQVELQNYTFSSSLNTMQFIFKSGITQRGGECVYDESTKTQKSLDSVRSIQMTLNGETLIGTFSDRIVIDDRPSYNKVSKLVDQEITQYGLDNQNVYISITTAYFQQSVVVDPNFGVLVTAQSDEEACKKPFEAWKIGTIVACSTIGVAAAGTTAVFIKKKKDQAAFMTKMSSLNRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q377</accession>
    <name>A0A8J4Q377_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_000733</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000015">
        <property type="protein sequence ID" value="KAF2077932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q377"/>
    <dbReference type="OrthoDB" id="197206at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17332">
        <property type="entry name" value="MFS_MelB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000183">
        <property type="entry name" value="sodium-dependent lysophosphatidylcholine symporter 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039672">
        <property type="entry name" value="MFS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11328:SF24">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11328">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13347">
        <property type="entry name" value="MFS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="288"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="362"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="428"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="489"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="30"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2077932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="536" mass="59646" checksum="0FFA7ACCD48BA9A8" modified="2022-05-25" version="1">MLEPGIILEETENDTRPLLQETSQATYYNSGSNPDLTGAAPGSTSASKPHIPHDPLSPQPYKLSRFEKLSYGVGESGLSVFQMIKGFFLNSFLLSVAQLDPFYSAIILFTTKFVDAFSDILVGSFSDKTKSRFGRRRVWILFGSLPLAIFYTMLWYVPDTNQTLKVIYYQFVVIVLSVAYTCVAIPYSAMNADITSDYDERTELAGIRMISLMIGGLISTFTHSIIIKIFKSTLDNGVVVYDFQKGYLLSGGIWGGIMLIPLAITFFGTRNIPTQSFKDSHIPFFKGLVVMFSNRAFITVTLLYFFCQIAVQFVQNNLVLYCKYVANAEDSFPYILATLQVSVSIFIFFWGKVSTYIGKKTVYYIGGVFLLGEFIGLYFLPANNKYLLWGMAAFGGIGVSVAFLIPLSMLPDVVELDELKTGERREGLFYSLFLFFQKLGLSVGLAVSNFALGLAGYEAPQVVDGTDGLSTEIIDNNVENPDVIKALRYLVGVVPSIIVFFSFIAVFFYPISKNSHSHIKELLKRKREGATITVDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3D1</accession>
    <name>A0A8J4Q3D1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphopyruvate hydratase</fullName>
            <ecNumber evidence="3">4.2.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_000155</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Mg(2+) is required for catalysis and for stabilizing the dimer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the enolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000003">
        <property type="protein sequence ID" value="KAF2078530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3D1"/>
    <dbReference type="OrthoDB" id="1739814at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.10:FF:000001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.120:FF:000002">
        <property type="entry name" value="Enolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF1">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00178">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enolase N-terminal" evidence="11">
        <location>
            <begin position="3"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase C-terminal TIM barrel" evidence="10">
        <location>
            <begin position="139"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="341"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="368"/>
            <end position="371"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="392"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2078530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="429" mass="46296" checksum="107F32F5B66455D7" modified="2022-05-25" version="1">MSIKSIKAREILDSRGNPTVEVDLFTEKGMFRAAVPSGASTGAYEAVELRDGDKSRYLGKGVTKAIKNITDIIAPAVIGKSVSDLAALDKLMIDLDGTPNKSKLGANAILAVSLAACRAGAAEKNVPLYQHIADIAGTTIRLPVPAFNVINGGSHAGNRLAMQEFMILPVGAESFKEAYRMGSEVYHNLKNVIQARYGQDAINVGDEGGFAPPILSNKEGLELLKLAIEKAGYTGKVKIGMDVAASEFKVENGYDLDFKTKNNDGSQVISGEKLADLYREFIAEYPIVSIEDPFDQDDWESYEKLTKSVSVQIVGDDLLVTNPERIRTGIEKKSCNALLLKVNQIGTVSESIEAALLSRKAGWGVMVSHRSGETEDTFIADLVVGLGTGEIKTGAPCRSERLAKYNQLIRIEEELGAKAVFAGEKFRTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4M6</accession>
    <name>A0A8J4Q4M6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_000543</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000010">
        <property type="protein sequence ID" value="KAF2078159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4M6"/>
    <dbReference type="OrthoDB" id="21204at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044600">
        <property type="entry name" value="ATL1/ATL16-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46913">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46913:SF1">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="6"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="81"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="81"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2078159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="504" mass="57765" checksum="D3A1DFE3DB6A22A7" modified="2022-05-25" version="1">MSDLECSICCLEFSTNGELRPHRLPCSHIFHKSCVYEWVRDKKNCPICRKEFTNSKVDDILCYDHVISSLIAKIQEITLKSTPPTTPIKSDSLNEQPIKKDIDNNNNSTIISMGNSSSSSSNIDDNSNSNISSTFSIQSILNNNSNNKENYSDINNNQERIDPNNDIDNSNNIIDNSNLEKSNVKDVLNDYYNYNYTMGNDSKMYFRFNLDTQQQQQQQPVDIKPNSIKGLNASIQKYMNTTITNPVIVSTPVSESTTLQQQFDSTSKTTVSTFPNMTITTSPANRSINSHKHSFIKRPISHYDQWVCHVCTAFGKGLSEKYNCQDCGLDVCRFCAIEKQFETSPKAQSNGPFYSSLHPDPLYFMNGLEVYGQRAYCNACSAQSTDIYHCTTCKVYDLCSNCMDKTKKRSTLSNNNNEISHRHHHHQHIDSTPFDMKNSRSLPTELTIERSKFIKVESPFQIYDEISWNCNWCHQKFSESLIVFYHSDPYRDLCLACFYKYYAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q6M3</accession>
    <name>A0A8J4Q6M3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003578</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000114">
        <property type="protein sequence ID" value="KAF2075101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q6M3"/>
    <dbReference type="OrthoDB" id="659430at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="873" mass="99164" checksum="5E522CFBBFBC5E0E" modified="2022-05-25" version="1">MDIKGGLQNEHTKNFDKLGLKELVLPKGFNQRLDGQLPPTLHSLSILNTDYDKELVAACLPTTLTSLTCWCMSKSVVLDLSLHRHLVSLDVYSESVVSYPPLLESLVVRDAYDANAPGIRINRDLIKKLAIVDISRVVQDDQDQDYSAVFEDDDDGDYSAVLPNLRYLKTNSVHSDRPIPSSVEVLDLRDYHYTLKQELKSHIKQLHLSTTTSTLQRLVKPLPIATVSNNIAIYNQTLPMIDGYFKLWRNVYIRSKILDSLYDLIRTDLKFNSDGSLLQHMDRFGNYNIQISNSHQLGTVKQYTPETITLGTEGVKLDFAANDVIPTSARKLRCHYFPHHVYPSWITHLSLESWSEMEWDRVPSTVTNLTLYNKKQKVDFQGKLTSSVAHVRLDCLDGVQRDSIPASVTRLTLVNEPVNSCGIQAADLPATIERIQFINLSIDDKNSHEIPVDVASRLVNGVFYVYSKSTARVPPNTTHLFWVDNRYICCKEVTIPRSVHTLVLNHRFNSAILSLPPTITQMVFNGEFKQPLSAIAFPPSLRYLCFNHLEETIKENDLPNGLSHLKVNVKGRGGFDSLPKSVHHLELSFNGDLSSAIPENVRHLKFNYTRIMTSIPPTVQSIITSQLSKISFRQYGSFKDPLPPPFDDNILVHLHPQAYIFGKLNQFNRFIQPYSLGTNIKSISFGDEFNQVILVGTIPNSVEYLDFGRRFNQKLSTSVLPTNLEVLKLGPDFDQDLNDDLFPSSLTELVLHTTPIPRLESLVRLRKLVCKYYDGVLKILPNTVDDLEFLKPDYKPKYPRPEIEFPIQLVPPSITRLVLHPYMYIQSYDLIPLSIKSITLCESIQPSKIPHTIESVQLPHSFNRPLDTILQTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q7Z4</accession>
    <name>A0A8J4Q7Z4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_002387</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000065">
        <property type="protein sequence ID" value="KAF2076331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q7Z4"/>
    <dbReference type="OrthoDB" id="16389at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000030">
        <property type="entry name" value="Ethylene receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000037">
        <property type="entry name" value="Hybrid sensor histidine kinase/response regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000121">
        <property type="entry name" value="Sensor histidine kinase RcsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000155">
        <property type="entry name" value="Sensor histidine kinase TodS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000045">
        <property type="entry name" value="Two-component system sensor histidine kinase/response regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43547:SF5">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43547">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Histidine kinase" evidence="13">
        <location>
            <begin position="193"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="14">
        <location>
            <begin position="526"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="13">
        <location>
            <begin position="702"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="14">
        <location>
            <begin position="1069"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="963"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1008"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1206"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="647"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1210"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="10">
        <location>
            <position position="574"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="10">
        <location>
            <position position="1122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2076331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1261" mass="140145" checksum="FA8E5D963A0F0534" modified="2022-05-25" version="1">MLSTRDIIVPASTHIPDEEKTREELLAELNYARSLANSNRTARIMLDEMYQFVAILDIKGNLLDVNEPALQGGGMIRSNIEGIPFWDCRWWAVNQTTIDKVRDACKRAAKGEFVRYETDIFGKSAGTELITIDFSLMPLFDDKGEVCLILPEGRNITDKRLGEIEIERKNKELRTLYEKIKELDELKSQFFANVSHELRTPLSLIVGPTEKLLRENLDENVKRDLEIVSRNARGLLKHVNNLLDISKLEAGKMNLNYSMVDLSKAINIITSCFDILAREKKLNYTVVTPPIPLMAAIDSDKIQRVVTNLLSNAFKFTPSGGSVECKLEVVELKNETNTEPGFQISVSDTGPGIPTDLHEVIFDRFRQVDGTSTRKHGGTGLGLSIVKEFVTLHKGTISVSNLPKQGCQFSIRIPLTPDMNNALYKKTVSDSIIPTIPFSNSVFNNNNSNNSLTTSPIIGSNTSSNNQNQSNNRVITYSPNLLDTSDQKDTLGGVFSIYDVAQQAAEELRDKQFYQSKEDNIHHKPIVLVVEDNVEMNNFISTLLSQYYFVVTAMDGVEGLEKTKAIMPDLIVTDCMMPRMSGDEMVKEIRTNEKFDHIPILLLTAKADENLKVKLLSNGVSDYVNKPFSSEELIARIDNLITMKKAKQFLQEELESAGTDLSELISQLASKKRDLQTLVNELEKERTLLDNANRSKDEFFMNLSHELRTPLNGILGWSQLLKYDYQSTMDTDPTLKTGLETIERCAIAQNQLINDLLDMSLIIGDKLNLSFESVNLPNLIANAIGLISFSAQSKKMTVESNIKNTAILSNIRGDPNRLQQVILNLLTNSIKFTPEGGKIVIKLATVDDVTKISGHSSNPSFNPIQPAGDTWAVLKVADNGKGISKPFLSCVFDRFKQADCSSTRSYGGLGIGLSIVQNIVHLHGGCVYANSEGENKGSQFIIVLPISQSTINTECKLLTNNTNNTVANGSEPSSTSTTPISNDDTSSNQTTTTSICKKRNFDEISTNTNNNNKVKNTTTTTTITTTNPTTTTTTTNNTNNTQQELSDDHQLNSKVLTSPKSSNILKGLHIVVVDDLDETVHLFSSMLYKLGAFKVSTFLSVSEVYDFLCNCTDLPIDIILSDLTMPNEDGYALARKIRDREMCNAHKIKKVPLIALTASVSTSDKIRVLSSGFDSHVSKPINFPDLCNSILSLVSKYKKNQSYLNYTTGDSNNNNNNNNNSNDSSKFNYLTESTTEGDYLQLDNTKLDTFINETRPKKKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4QA68</accession>
    <name>A0A8J4QA68_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ER membrane protein complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_000808</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000017">
        <property type="protein sequence ID" value="KAF2077846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4QA68"/>
    <dbReference type="OrthoDB" id="28092at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058545">
        <property type="entry name" value="Beta-prop_EMC1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026895">
        <property type="entry name" value="EMC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011678">
        <property type="entry name" value="EMC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21573">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21573:SF0">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25293">
        <property type="entry name" value="Beta-prop_EMC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07774">
        <property type="entry name" value="EMC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035155081" description="ER membrane protein complex subunit 1" evidence="12">
        <location>
            <begin position="22"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="938"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="domain" description="EMC1 first beta-propeller" evidence="14">
        <location>
            <begin position="22"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="ER membrane protein complex subunit 1 C-terminal" evidence="13">
        <location>
            <begin position="757"/>
            <end position="970"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF25293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2077846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="971" mass="108848" checksum="6315EBF904CB91C7" modified="2022-05-25" version="1">MSTTPTLLVLFIGLIVGCCLGIYEEEVGLRDWSIRNIGQLESVFDYDQDLLVQSKLLVYNQNTNNYTNSFSLALLDKNTGITKWRQLIPVQEDTFEQILINDLDKDYFITISNGNIIRYWSRVNGELIWNQVIVSTNQCYQASFVNIDTLRVLCLDSVVFLSIKRGTILSSANIDSEKNYQSIPNTFYLYYRDTDNNVQFLPINYQNSKVDTSKSKHSVSLDLAAGDQVTGHLALEGAIVYTSSANNIHVLDIQEEKNGNALNVEKQTIALFSLIKEQGISIVGVDSVYPDNRFTVVLSNKSKLLLEYTNRRLSVITMIEQHYVMVRPSLSGQQQQLFIGSNKSDGKIELALATESAKNPQPLPMGINTTVNGDIESIFSTDKQGYCLVVTNDWAIHMIQYSSRADEPPILLWTREESLSSVLASEIIDFPLPELAKLEEFEYEFNSTQGFIQHFTRRITTQIQDLVSLFMGSSTDANKDVIINQDNGNKQRTPKMILLTTLAGKVFGLSSDKGTIVWSRYYSDFDRSRAESKIFITKKTLYYPPEAAIVISTTSKSSSTKSVVSFINPLNGKESSNKIINHNILHASILPIVDDHHHQLLMIAVDYPPNQPSPMVQVYPWNEYIRHHSFASMKNIVNYYLVNHTAATVQGYRIDSMANGLKGQLKSDPTWKISFGANHQILAVGSSNAHEIIQSPAVILGNRNLLPKYVNRNVISIASLDPKLSLLTITLVDAVSGITIKSFTHAHSAAKVSIVHTENSVVYAHYDLTSHKQHISVIDMFEKTVEWTQDTYSSLSTSKQDIAANLIIKHKTFDFVQGLIKTMRLAITTKGITSKNVLVGTVSGQILPIDKKWINARRPTPEEALPTDQEEGLIPYGPRLAFPPFFYITFNTTIPALRSIETQGTDLESTSIVLAYGVDVFCTLIAPSLPYDILSDSFNHIALIITSIVLLILTFISKNWKDSLALKKKWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4QAK4</accession>
    <name>A0A8J4QAK4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000649</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000013">
        <property type="protein sequence ID" value="KAF2078011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4QAK4"/>
    <dbReference type="OrthoDB" id="2121326at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035226324" description="Thioredoxin domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="49"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="150" mass="17157" checksum="276CAD39C39908B2" modified="2022-05-25" version="1">MFKSLLFFSLFICLFFQISQATTVNVPSATGWPLLLNSTFLATKYDRSDNKLIVIYFTKDDCPPCDVISPLLGPWLTDTQYSGVEFYEVNVSHFLNIPSDYEVSEVSSLPSMKFYKNYEATEDGYIKFLWKSSAPLLMYQDASTFLNANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UTX3</accession>
    <name>A0A8J4UTX3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010337</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001046">
        <property type="protein sequence ID" value="KAF2068338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UTX3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="180" mass="20580" checksum="09514884217B28C0" modified="2022-05-25" version="1">MKSNPFPSQFSESNQGPTFTTDTNDLKEIIDLVFGKMVDPFIQEIYKSPTTIYRRLKVDLVLGILGLQKHHVGNVLKSQEPTQPINQAILKRTVEYVKPNLEEGQYKDGSNKHCTAIAWQAKKDKTVYMLTDIPEPIMKSQEKALLRKSTTKDSKNNYSINQLVTPRVIWVFLSQLRGLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UU90</accession>
    <name>A0A8J4UU90_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003514</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000111">
        <property type="protein sequence ID" value="KAF2075166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UU90"/>
    <dbReference type="OrthoDB" id="17543at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="60"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="427" mass="48222" checksum="0C4BE35B5C1F35DA" modified="2022-05-25" version="1">MGHAQLGRRSSSCKMNIKKSTTTTTTTSKSTILNESDVEDSSSSSNASDTSSDDSDDQDTSSSDDSSSDSSSDSSDSDDDVKQSRNNKKIKNNNNNKIDSNNKVYYGEKTCEKVYKDKSGKEKSCFHKAYFSINNNKSFVCGVHKGTDKLAKPLPKRDKKEAELLKDEKFKAENKVIEQHAKQNKAKNLEGRVVCSKLRMMKSPDEIEGYRKIFPNFKHGGRKDGIGMPSLSPMSLGPFNSGQPAAPIALNIENFHQGSKCFKRDLEKNGEVGATYFKGRDAFYTDKTPHRHKYMNPKTNKPEIPEFFVWVDENNKIHKLNALDCRQFYCHFYETLARQQADYQKLVELKQSGVNLQIVGYDARSIPAGEDAIRKEYNNTRAPFGHELVLYSMLTIPNPDNYPWKNKKNLVTPAILQNLSNVDEIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UUK7</accession>
    <name>A0A8J4UUK7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">CYY_003129</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000094">
        <property type="protein sequence ID" value="KAF2075586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UUK7"/>
    <dbReference type="OrthoDB" id="10261027at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000060">
        <property type="entry name" value="Serine/threonine-protein kinase isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF288">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="831"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="157"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="228"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1197"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="626"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="1115"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="126"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="157"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="257"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="271"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="286"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="340"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1199"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="954"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="858"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="959"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="972"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF2075586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1233" mass="139430" checksum="CABFC828119625A9" modified="2022-05-25" version="1">MEGPLSITTKKQTRLDNNIINNNNSNNNSNIDNNNITNGGSTAEITKKATENNKNSNNNSPEKSHKQEESPPSKNASGDRIYTMDTSLTFDNTTKKRKTIGSNQSSGDDSLDIENDRPIYHRNKRPSISINISPSPTTDELNKLTLNSNSNLVEFNNNINNKNNSNNSNSSTSSSNISLDISNNSINNNHTTTTTTTTTTTSTTFNNQQQNPNEEIEEINRERLERDKMLHEENDIDPYTTFKYGDGEEGEAFDSLPLHHDHEKLTDVSQNNSPSRNHGNNNDDDDNHHHDHHCEEDDHCNKDHDNNSDHTYDHQSTDDDTAKSDMEKDGSESARNSNETLKDEIHMDDNDNDGHNSPQHNHHGKDESTVSTPQHQYMMEDDDESDEEDDVPLRIQTAANTKQPKLSLTNCWLKIIPADVFSLLELRDLDLSANHLKKVSKSIGSLVHLKRLRLNHNQLTTLPKELYNLPRLTTLYLNNNNFKIVPKEISKISTLKLLDLSFNQITDISPQTNINQMTNLVELRLRYNQLSSLPSNMMEATHLQVLWLEGNRLPLNKQVLKKSPAEIFTFLKQYKPPTKKVHEKHPKIGLDLQKSPPSIDAASTIAVAQVFAQKEMDTKKRKKENLVDIKKQQIEAEMKLKQLEEELLIANAKATKFEEEAAVLQQHFNNQPTPPTGTGATGQQQVPPTQVVANLPQILLNQPHLWDQQQNPNSPPNLITPPTSISPVLNGQQMVNSLSQISLLSPTANNPPSPIPPHLIQTNVQQSSPTHQQQQMHQIPVQQQQVQQIIIPNPQQQLIDHQFAVPISPAQQHKQNTEDKPFEWEVPLSEITLGARIGRGGYGQVFRGNWRGTEVAVKMLFNDNVNLKLIIDLRKEVDLLCKLRHPNIVLFMGACTEPASPCIVTEFLSRGSLANILLDETIDMDWGLRLQLGFDCARGMTYLHSRNPIIIHRDLKTDNLLVDDSWQVKVGDFGLATVKSHTFAKTMCGTTGWVAPEVLAEEGYTEKADVYSFAIVLWELLTRLIPYAGKNTMQVVRSIDRGERLPMPDWCPPAYAKLINRCWDTDPTNRPSFPEILPIMENMISEFQIGKKEAIAAGKPIPYVGPLDKDPSIVIQKQIELQHQQQQQIQQQQQQQIQQLQLQLQQQQQQINQQQINQINHINQFNQAAAANQSHIQVQLLQPSSLVKQVVQLPIQPENNLNNNSNNNNNNNNNNINNNENNNNNAFYHKPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UUN0</accession>
    <name>A0A8J4UUN0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_002989</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the formin homology family. Diaphanous subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000089">
        <property type="protein sequence ID" value="KAF2075676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UUN0"/>
    <dbReference type="OrthoDB" id="19402at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051425">
        <property type="entry name" value="Formin_Homology"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725:SF1">
        <property type="entry name" value="DISHEVELLED ASSOCIATED ACTIVATOR OF MORPHOGENESIS, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725">
        <property type="entry name" value="FORMIN HOMOLOGY 2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FH2" evidence="5">
        <location>
            <begin position="495"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="52"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="342"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="410"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="875"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="58"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="345"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="420"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="450"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="884"/>
            <end position="896"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2075676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="896" mass="101550" checksum="E386E4C1334F2451" modified="2022-05-25" version="1">MLFLPPSNELIRVDSTTSSNNKNNAAINSMINNANIANISINSLSPPIAVISNNSTPTPTPTPTPPAPVTKPTTTTSSDWGSKFSSLFSQITKRKKSNYEIEIDALKDALALNKPINNEEGILIYTLDKQKKYIHSLNELEIGYIYLTEKETPIIRQNEDEVILANKIIIQANTLKKYSLKGILQTDVTILALESFTKRKMAQGMLFLEEDGSICRIFYRMQDLILNTIYCNHDDTVMLQRLPNSDGGIRFRFQGSFSPHQIEQAMIAYANPSDPLTWKKIIVLREQQHHSNQQFSGDNIATRFLSKLSTKTKEQTKDLKTKRETTILKLKNRMRIDWEWKNENEESSDEDEDKENNGNSGNLFNSVVDKVKEKISSSKNTSTNKSVAPISTLAEILLSSKTKKELLGKIDNNNKPLVPVKREEESKKQQEQVKEDEIKVYEQTSTTTTTPPPPICAPPPPPPPPPPPPPPPPPPPPAPPMMKLTKGGLKSPIKTPIKNLNQKKMRQLHWNVIPGNRLKETFWENLSPVKGNENDQTLVESWFSLSPLAKEKKEASTNNAASNNTATNAKNTNTISLLDLRRANNICILLSQFKLSYSAIREAILCFDESKLTVEQLIALDAMLPITEEEQITLTAFDGDRELLGNAERFYLEMMNINRLQQRIQSFLFKLEATTIMAEIDANITLVAKAIEQVRNSQQFIKVLKVIFHIGSILNRGTYLTATKGFRLDSLAKLSETKSKDQKHTVVDFIEIYIRENKPELLHFYTDLDLLDRVGNLSLENLIEEIANIELKFKLIQEELEYHKEYPMTKDNYYEIMDPFHRDTYANFNALKEKSLTTMKQFTECCCYYGEDPNSNSISSKEFFSNISKFSNAFKASSMKKKSPPSSSTSSTTKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVP5</accession>
    <name>A0A8J4UVP5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">CYY_009681</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclin family. Cyclin C subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000776">
        <property type="protein sequence ID" value="KAF2068998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVP5"/>
    <dbReference type="OrthoDB" id="10266018at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18177">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6F_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18178">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6F_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20513">
        <property type="entry name" value="CYCLIN_CCNC_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20514">
        <property type="entry name" value="CYCLIN_CCNC_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000076">
        <property type="entry name" value="RNA polymerase II holoenzyme cyclin-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.610:FF:000002">
        <property type="entry name" value="V-type proton ATPase proteolipid subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000245">
        <property type="entry name" value="ATPase_proteolipid_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10263">
        <property type="entry name" value="V-TYPE PROTON ATPASE PROTEOLIPID SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00122">
        <property type="entry name" value="VACATPASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0010">Activator</keyword>
    <keyword evidence="12 17" id="KW-0195">Cyclin</keyword>
    <keyword evidence="8" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="11 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="109"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="19">
        <location>
            <begin position="208"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="19">
        <location>
            <begin position="309"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU363060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAF2068998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="419" mass="46576" checksum="8B8A3788B889F8A5" modified="2022-05-25" version="1">MSSAVLPDNGYFDQGTYFLVTISPQTWAAVGVGLSIALSVLGSSWGIWVTGSSLFGAAVKEPRIRSKNIISIIFCEAVAIYGIIIAIILQGKIKPHMNVADIGGDYLAAYMMFGAGVCVGLCNVFSGLSVGIAGSGCALGDAQNPTLFVKMLIVEIFAGALGLYSVIVGILMVSNVTLDRNKIDESNGKDKQYLSALEIKKLKTHFCFVIQNLGNALKLRQRAISTAIVYFKRFYLKNSFVDCEPRLIAVTCLYLSSKVEECITQAKKCVTKMKELDHSFNYTMNDILECEFYVLEELDFCLIIYHPYKSLPLFLANSGLEADTIEVVWGIVNDSYKTDVCLMYAPYVVGLGCVYLASYLLKKDLKQWFSELNVDMKDIWEVSRELSDYYDFEKSFLSPASSHDSPEFIYNKLPIRNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UW86</accession>
    <name>A0A8J4UW86_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009342</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000683">
        <property type="protein sequence ID" value="KAF2069343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UW86"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="92" mass="10487" checksum="49ED6B4591BE5F23" modified="2022-05-25" version="1">MPPHLKTLCFILWPGSPRGNDTLAVELEIVKIVFKKLLRPSVLPGGLKNLFLDDFNNCGTYLQEHSFKDPLNNLKSLSIYSKRTIQLINLYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXK0</accession>
    <name>A0A8J4UXK0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Histidine kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_000086</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000002">
        <property type="protein sequence ID" value="KAF2078586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="19417at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16922">
        <property type="entry name" value="HATPase_EvgS-ArcB-TorS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000010">
        <property type="entry name" value="Sensor histidine kinase RcsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053159">
        <property type="entry name" value="Hybrid_Histidine_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43642">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43642:SF1">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1315"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1347"/>
            <end position="1367"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="171"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="2022"/>
            <end position="2244"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="9">
        <location>
            <begin position="2499"/>
            <end position="2619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="91"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="321"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="436"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="94"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="321"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="342"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="437"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="511"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="524"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="2549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2078586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2633" mass="295893" checksum="9D958662E49D8935" modified="2022-05-25" version="1">MNTTWLQDFHIGEKIQEEKLFSVFKAIHYGNLSGNGISHNTTTTTTTTTTTTTTATTTTTTASTFTPSGNLIAGSLTSSIPFSPPNSSHIPNYSLLSRDPLDSPSPSPSSVSSITSQGSLNNSNSSIENISGTGCNAGINGCNSSQFKSITNSITATKLQQQQLADGANIPQIHQTTNEGSVGSASSGSSSNFLSSPTGYFNIKVPSSVQATKRTANDHMFSHQKVFLKFINSKYPSMEIISLFQREATILSLLKSIDGVAKIIRTQSELGNIVAIVFEEAGFQPLNQIHPDVDSPYLHNFDLNNSTSILNSIPKLVNNNNSIGSNNGSSGSNSNTLKPKQTTPTITPLSSSPLLTNTYHSGNSMTNRYEMDIDTFLNMAIQLSSILEDIHLRGCIHRDIRPANIYYHPNAHQIRLANFQFSVIKHLERLDNSQIHSYQNDSNNSNSNLSNSNESVSNTPTTPTSLSSSNSNNQQQQQSNQSTTPTSTSTSSSTNISPIPNNISTFSPPSASSIASENLTVAGSNLNNNSTNYNSNTSNNNNNNNNNNNNNSNNNNNNNNVAGNNNNPQPKNKKKDLSFLKNFPDLSRLNSASYYYTSPEETGRTQHPVDYRTDLYSLGVTFFELLTGRLPFQTTDLSELIHSHLAKKPPIVSELNPKVPDILSRIIDKLLKKSPDERYNSAYGLKRDLEICKLQFKSSNNSSTISPDFKLGTFDVLHRPFIPNELYSRESELKQITDAVNRVKKGSKELVVVRGVGKTSLVYSVKKVLPLGPRFISGKYDQYNVGVPYSAITEALTEMVNQILVLSPMELEFYRDKLFRALGPNIGVIVEVIPFLELIVGPCAKAPSLPPSEAQNRFDIVFKDFLRVFSEEGHPLILFIDDLQRADPPSCRLIKLLLDKNNNIKYFLFITAFRENEMQDAFIAPILEESNLRTTEIDLKPLQLNDINSLVSTSLRRHPNDTVAVSEVILNKTHGDPFFTIQFLKTLFIESLLYFDLQYDVWKWDIKKIQMRQFTDNVLEFMIENLKKLSPESQRILHLAACIGCQFDFDILTIVSEHNEEDIIQLLTPIISQDLIIKSSEKKYNFVHDRVQQAAYSLVFSKHLNEIHLNIGRLLYKNFEKDNDILSSNIFDVVNQYNFGIKLITNKDETLKLAELNYRAGMKAKNTTAYAMANGYFQNALTHFDLENSWDDNYQLLYKILVEKAECDFMVGNYEESDRLLEMCITRANNVEDKGEAISLRVQQYTLLEKFKDCIVSGLEYLKSQGIDINIHASDEELFKEYCEFKEKIGNRSFMDLLLPTIKQPPSNIKVCLKMMGIMIAPMFFTNIATLTAVLIRFANITVTHGLSSWSCVGLVMFALVACIGRFDDFNLGYDCGKFAMALKDLYGEPSTKGKVTMLYGSFINHWRSHISTNSSFIKQAFLESIEAGDLGYACYSGVHLIFSHILAGTPLKETYQLSKKYHSFMTQNYFKPMYVIIKSHQNLLIELIGAENLDEQDTLGEYGWNEAAYEEDLETMEIECPKCFHYVFKMQFKYYLGEYEKAWEATTFTDKYINGMYAHSPLSFYYTFKSLIILALYEQYSEQEKVEKLNVVYYYLDKLQINANQSAENFSAMIPIIKGELFRVQKKYEDAMEMFSLAVTLSQNYKFINLEALSNELAARLYIHLKKFATAKAYLLEAHCCYSMWGALYKANLLELEYPNIFNPSSNRTHPINRVKRFDFFSPSSANNGPPSIAKPASGSNRNSINEPILSIRRKSQDGSFSTIKNPFTYPQVDAVDLCSVITVSHSLAEEIYFDRLLKRLMKVVVRNAGASRGFLMLIEDNNTKGLFIKSFNENEFGDLYVAASSIVYGDKVKVEVYINSNLSPIGKSPTESPLMKPCSIPVDSQEPHVKPMRSDGKWNICASIVNYVRRTQSPLLIPNAIEDKTFSEDPYIKKVKPKSILALPIVHQGKLVSILYLENNFTSAFTYSRMEILNLLSSQIAISFQNARLFLQSNNLANQAYLAKEEAEKANRSKSDFISNMSHEMRTPLNHIIGCLELLTEFPMTIEQKELLYISQQSSECLLYMVNNILDLSKLEQGKTMLTLSHFNLIPFVEDTIAAIATIAHKKGLYIGLIVEPVKMFNVPIEADINLLRQIIVNLLNNAIKFTHSGQIYIKLGILERESTFSTSFEIIDSGIGIKMGDFDKLFQRFSQIETGPNRAFDGTGLGLSICKQLVELMNGAINLSSNFGEGSNFHFSVDLVKSNSLKCNVNYHIKPHQMENVEIKIFEQNPIILDQITNICHTIGIPSDSMSTSSVYDLFDLSPHLESLKDSCKGTKTMVVVLGLPLNYPESDFITIIEHIRTLKARYCAQLEFIVLSQLLSQSVSISRQGIKFLNRPIRIESLFSHLNESLTKLGSKSPSFSSPLHKSKSAFIGTSPSLLSPTIDSAFHNCLQSFNGAEKEKEKEDVHKIKISCENTKLIKIQHTDGSNNCSSTMVKHSHHVVQQQQEQHKAAMPLILVVEDNEMNSNILFRMLQKLGVNNAHLALNGLEGYKKAKDNNYDLILMDIQMPIMDGYEATGKIRTMEKQNGSQHTPIVAMTASAMIGQRDACILAGCDDYFCKPLKQKDLEVILKKYIKFNTTTPTTNMKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXU3</accession>
    <name>A0A8J4UXU3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_007185</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000369">
        <property type="protein sequence ID" value="KAF2071490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057709">
        <property type="entry name" value="DUF7949"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25820">
        <property type="entry name" value="DUF7949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035152272" description="EGF-like domain-containing protein" evidence="3">
        <location>
            <begin position="18"/>
            <end position="1378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1335"/>
            <end position="1358"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="7">
        <location>
            <begin position="431"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="6">
        <location>
            <begin position="660"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="5">
        <location>
            <begin position="777"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7949" evidence="8">
        <location>
            <begin position="1042"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="4">
        <location>
            <begin position="1109"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1003"/>
            <end position="1034"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2071490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1378" mass="154632" checksum="86051AF0D720D7D4" modified="2022-05-25" version="1">MIKIFFFCFCLIVFVHSLKISVVLEKEENNLYANSLSQCTKGYLVLFSEIASPITSIDHSCISTTTLNQTIDSSNLLYNFEFQNSIGAQSCEITLITQLETVGYTFNFICQEMPIPIIKDIGSLPQYTQKFGTFSFNILYEYMFSIENVPEPNSQLSINYLVNDTNWSLGLSKINYNTYIIEIYPIYSANTIFQTTVVKVTLNFQNNYEHFFEIELKNPKSNIEMTDYNLAPIDNPVMAYQVLGSFNIKSDTPSKLIFGFEISNMMLFCLNYPIYGNPFNSTYFIRLYLGMDFNQNLDWVNYQGNVKQILTSKNINIIAIPPIDDSPGDIKSKVSDPNPALGGNRILSYLTTNTKFERESYSFLNMVIEDQVYLDPNLQYLNDGAFSLIGNFSAFNRSVDMIVSKYYNRENIWFGILSAISRNDYIDPSSIDLLPPIINDWELIALPGFGEYILLRASITDNISGFAAIYEPNPSSTAIIMSCQNLVSGTIQNGVYEVVIKNPLFSIQEFYISDYASNTNTYGSKFLPIMEPFVNLNFSNPRNYNYDSLDFQVLNANWSHNDIDLSLRSCSVIFSFTLANPDKEMSPCFQLGLEYPFIEIKIFCGTWNETSNQYEILVTLPMRMLTGVLSYSLFYYKHISSDYLNLAFPSSELRIFSQDADMLGPEIIDLSPIDGEIGWTFRVYDKLNGFASGNITITGSLYQIENVFILNPNDEIHEIRVPLLSPCVPQTYTITSAFLVDNGGYVSDIVNVFKNYINVDLHKIEITCPAPIPLDPPILKSFEFNPQTIDVGSNNRNVTIKFTVEAPIGNALIDRTPFVYLTSLYTITKKEATFKSSSNFEYFYECEMEVPYGFGAPEKSIFSLYGIMDITATMIGFTSSDLDRLHFDYILDTTEFTKDAHPVIESSSDITVRGGKLLIIGNAFGSDIGTLVVNVVYNDGIIDPKHAIFLSPTSIVIENVRPTTLPFKIQIAKLGTNSETLYSNDYWIKPRLVPGFPYTDSSSTSSIGSSTSDSSVDSSSHSASFGSDSSSNEIIPTNPPNPCINSCGGPTQGRCASNGCVCISPWVGADCTSKVIIIPTPSINNSLPTTNFSFPSENNAENIGLTGIISIVELQELDKNDQILFRYPFAQWVWTNISTFGTPEKYLYSANITNSNLTTYVNVTIQYFEKQENISFAGEELIMNPYSIKYNINISSYSFTNSQNSLQLIMKLTLESNESDGCSAIEKGNTTLSNSDFVKLQINNHSLYGRFVKRGIIDGRISSISNQVLENYHQDKSTQFNDVISFMGIKIRSFRSMVQLDPDFSVLLDQNGASSSDSTSSICSSKKKKLSVAQLAGIIIGSILFGAVVIVASTYFIYKNLKEKRFEKGFEKKLKNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UY29</accession>
    <name>A0A8J4UY29_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_006730</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000323">
        <property type="protein sequence ID" value="KAF2071955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UY29"/>
    <dbReference type="OrthoDB" id="10250130at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF18">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="143"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2071955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="552" mass="62626" checksum="4BF8C123DC331A94" modified="2022-05-25" version="1">MPALSKACKKTNTYNNNNNLNNMNNNNNNNNSIQGFLNNIFNSIFKKKKNNVQPSQQIANNEKSSSDSLGFENSICASNSSSSKYSKFSLLDFNLNSNNSSNSSIESEYINNNSNTRVLSNRFSKNNSSKYLNNVFELQEIDIFAIQELPEEVVVKISTYFSVEMMYNMSLTSSNFYRLMNDIGLWKLKVQEKYSGKSNQHSLLSNKMILDNQWKNYYHIKSLISQTKGNVSSTVLKTKGSSPSARYQHTGTVIGNSIYYIGGQETQVRRFDDIFKYDSEKNRFYRIECSGTPPPKFARHTSVAIQNKIYCFGGFDGSGIYFELSIFDPEKLTWTTATVSGNPPRSRTNHAAAAVGSKLYVYGGINRDGRWELQDLDEFFVFDTTDLSWREVKATGDVPTARCGHRLVSIGKKLYMFGGGAGDSWRERYNDIHIFDTETNVWRRIESPQPNVVHVCTFSSVFVMGSFIGVFGGQHLIRGKVTKKLYFFDVISETWSKQEFSGNSPNPRDMASANVLNNRVYVFGGYDGRAMDDMNVFDLSPKLKSLINTKFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZ51</accession>
    <name>A0A8J4UZ51_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PPPDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_004624</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeSI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000167">
        <property type="protein sequence ID" value="KAF2074055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZ51"/>
    <dbReference type="OrthoDB" id="412286at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101005">
        <property type="term" value="F:deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.30">
        <property type="entry name" value="PPPDE domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008580">
        <property type="entry name" value="PPPDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042266">
        <property type="entry name" value="PPPDE_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378:SF80">
        <property type="entry name" value="IP06716P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05903">
        <property type="entry name" value="Peptidase_C97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01179">
        <property type="entry name" value="DUF862"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51858">
        <property type="entry name" value="PPPDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPPDE" evidence="5">
        <location>
            <begin position="54"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="204"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="268"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="211"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="280"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2074055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="344" mass="37311" checksum="1F9781DFB0E22150" modified="2022-05-25" version="1">MNVKSLKDLKQDNKDPNTPTNSNNNNVVSLNSSSSVSSNGSMATNYSQQPSSPNQININVYDLHPINNIMYQFGLGAFHSGVEIFGVEYSFGGHEFSFSGVFEIEPKTANGVTFRETICIGETPYSYKQVQNIVDKISEEYSGLSYHPLQKNCNSFSQELVKRLLGANNTTPFPAYINRLAHFGNFLSCLLPSNLPFLKTPNSENQKEALTSTSSNSSSSSSTTTAAGNTFPGKGYALVSSLSPPFTPAMESDQNGTNISLSTFRQQIENEPSSDDEDNNNNNNNDGEQDSSSNSSSGKSGNNSDAEEQRRRKLLSAANKRLGVGTTTPPAANEEPETAPFMEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZB5</accession>
    <name>A0A8J4UZB5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UBA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_004345</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000151">
        <property type="protein sequence ID" value="KAF2074370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZB5"/>
    <dbReference type="OrthoDB" id="20614at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066:SF21">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="125"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="7">
        <location>
            <begin position="272"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="223"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="223"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2074370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="309" mass="34200" checksum="0769E94F55E6DC6B" modified="2022-05-25" version="1">MYSSGPSGLQNAPVTKIMLFGSLGVNLMYNYFMIKKGGVVSWLPASVSLLVGVIERTQILSMISNQFLFTNVPELVFGSIFIYSFRLFERMMGSSKYTAYIISSVSITTLIQLSYSILTGEKAKASPFSFIFSTLVLFYRDIPPTIWFKLFGFKLTDKFFSYALASQVLFTFPQNLLASSFGVLSGLIYSSRLVGLTNLSFPRPVNNFCTKWILPLIQSNDTTTRGGNNRRTTTGTSSSSSANRNTRNGGPVFQNLSTNTYYQAPPPPTPIAPSEENIETLVNMGFSRARAVEVLQRTNNNLNEAAVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZU2</accession>
    <name>A0A8J4UZU2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_003838</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000126">
        <property type="protein sequence ID" value="KAF2074850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZU2"/>
    <dbReference type="OrthoDB" id="10251136at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000022">
        <property type="entry name" value="Fidgetin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000093">
        <property type="entry name" value="Fidgetin-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF17">
        <property type="entry name" value="FIDGETIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="504"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="275"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="324"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="219"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="327"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="344"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="399"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2074850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="743" mass="83667" checksum="96D974B5D8C4A9E2" modified="2022-05-25" version="1">MDRFNTYNSSSSNSNNSNVGVHIRAVNINSVDSHVAPQLLRTERCLLEHLREQERRLTPGTEQHLDVVEKIYRCIQRRVGYLGCLQYHATQYQLSLHSKQQTTPLLTSGFLVQQREIEHQLSELQQQSNLCIDIISNKNNNNNNGKSRYRLVESMLNKQDINTITSSVSSLALPPALPSTSPALQPNKSLITFDNCKPNTNLCLVNFSIFKDRETQRVEQIKQEQLKQEQLKQEQLRQQQQQQLLQQQQLLQQQQQQQQQQQSKQQYQYQQQNYNNSNNKQNNIKVDDEEDEDEVQNQFKSATVLLVENDKKKAQQGGFVSNSIKKYSSNTTSTYTQPTPSNQASSINSKQSNTAQPSSGAPVVKKTLGTKRKGFIPPVKQDEDAIQIDDDQSSKKLKTTNNSSNNSNNSNTSKKTNTNTGNGKKDAETNPNGFDNVNGVPLDDEKLVNIEPIMLEKICNEILDLKSRLSWSDIAGLKEVKEQIIGMATLPLLRPDIFKGLRTPPKGLLLFGPPGTGKTMIGKAIANEVKATFFSISASTLTSKWIGEGEKMVRALFAVARCYLPSVIFIDEIDSLLTQRSDGENEATRRMKTEFLIQWDGVSGNAEDRMLLIGATNRPDELDEAARRRMTKRLYIPLPDLESRIALVNNLLSKENHSVSQEEILTIAEKSDGMSGADMKSLCTEAAYEVLRGVGDISQIDINHIPPIQLKDFIEALKKVKPSVDQKELKSYQDWNIKFGALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V019</accession>
    <name>A0A8J4V019_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_010064</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probable methyltransferase.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000908">
        <property type="protein sequence ID" value="KAF2068612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V019"/>
    <dbReference type="OrthoDB" id="20143at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050869">
        <property type="entry name" value="H3K4_H4K5_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197:SF291">
        <property type="entry name" value="SET AND MYND DOMAIN-CONTAINING PROTEIN DDB_G0277331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="6">
        <location>
            <begin position="25"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="MYND-type" evidence="7">
        <location>
            <begin position="68"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2068612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="480" mass="55447" checksum="48FBCF4E04FC38CE" modified="2022-05-25" version="1">MRSSRESVLKDVQRITSEYGFGNKWGIKVDRSPSYGGGLGLFATRDFKENEDILTTHAYAHATNQSHCDGCWLSKEDGEIDKLFKCSQCSFVCYCSRECQVKMWPIHKLECKNMSIMLKRHCAPLPGMILLTRLLYTHRANITRNGSLELINNLDDLWANDNKMGKPMEYLLNFVQATLFYTQEPRSTDGALLNYTKKIESHVTSYGEQNIMILPFLAFINHRCISNIFKVHGHSNKLEMTIVASRDIKKGEELYLDYTKLEYRKERLEKLHKYFLHKCLCSICREINDTGSKEKYYRCQDCSCNGRVDIRWKSPSLKQYHAICLKCGKEYDAEELEKKGNIIKSNIKEEFNKAPLEIKEMMIKLYTQHFHVLDFFLAEELDKDALVSLNDVFIFKRIQLQLDHHFDPCSLTSFGIVGDFYLKLELKNPDLAAYVNGFTSKTAGGLTVPPKIKQALSNPDCYFDKTLLDQPKYGGSVVRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V092</accession>
    <name>A0A8J4V092_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Set2 Rpb1 interacting domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_009749</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000795">
        <property type="protein sequence ID" value="KAF2068927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V092"/>
    <dbReference type="OrthoDB" id="21636at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1740.100">
        <property type="entry name" value="Set2, Rpb1 interacting domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013257">
        <property type="entry name" value="SRI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038190">
        <property type="entry name" value="SRI_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08236">
        <property type="entry name" value="SRI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Set2 Rpb1 interacting" evidence="4">
        <location>
            <begin position="529"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="356"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="484"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="41"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="61"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="78"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="97"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="126"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="141"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="183"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="199"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="280"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="369"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="393"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="460"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="484"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="499"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="511"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="699" mass="80953" checksum="246130841902F64B" modified="2022-05-25" version="1">MARKSDSDSKKKKKIIIEEDDEDHQQQEDTEKGDGQQEIIDQVEGDEEEEQGEEEEGHGGKKEKKKKRHHRRKREDKENGEEDSDEKKDDDDESKEKKKRRHRKRRHHKSSSSGDGEDENEDAEKKKHKRHRKKKHRDSTTEEEEEEEKVEGVDDEINAFEEEGEEEELVIQDSPKKKRKVDTDEDYVDEEDEEEHEQEEEKQQQKVQKKVEEVRDDFFEKHLAHEEKEKERDNRYNSRDRDYKSNSNSRDRSSRDYDRNYESNNNNNNRSSRDYDSKSRSSSSSSSSSSNNNEKKKSNILPHDDFVNRVIAPPPSVFIGEYKPVEYSAYKSKFGERIFNMVPEQQLPTPHFITKKEDNHFNFDGGPQNNYNKNNSNSSNNNNSHFSFDEPQNNYNRNSYNNNNNSNSNNFNSNNSYSSNSNSYNDYKNNSNNNSSNKNSSSSGHYNFDDDDDDKKNNNSGFNQPKQPEYLTNFSIPDTLVQSSLSSTTTKTTNSTSNRHSHSSSSSHHKPSGEKKKISSKDHKEFKIKISKIVIGKLGKYYKTKITTKEDFQYLGKKFTKLVVEKEHGNTSITKDTSKKIEKLIDNYFEKKLVYDRNKDKDRPEKVLAKSPFADDGSSISNANTTTTTTTTDIIGQDIIDQEKQTTTTSTITVTTSSNDTSELSLKEVPKSFQPSEMIENANTLPSLTTTTTLNPDWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0J7</accession>
    <name>A0A8J4V0J7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002878</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000085">
        <property type="protein sequence ID" value="KAF2075795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0J7"/>
    <dbReference type="OrthoDB" id="17324at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="65"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="157" mass="17851" checksum="7F4B1A6033866EC7" modified="2022-05-25" version="1">MGNAQSIGGFTPEERKDLIAKTSFSKNDINKLQEDFKREYYGDQEGLKLDCKEFIGALHLFTRAPIEEKLGCLFDVFDSDGSGFLEGNETFRLYAVLISSAQAAGKYQVDEAQKIIKNVFDTKPRVSRSEFILTLSQNQRFTTILGFYPNYSKTNLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0N4</accession>
    <name>A0A8J4V0N4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009212</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000656">
        <property type="protein sequence ID" value="KAF2069467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0N4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="274" mass="32587" checksum="F9D80485C3940882" modified="2022-05-25" version="1">MGQNMVKNDKKEEENQINLINNNQGEEDENENLPDNEDYEEGANSNQQYAHMSKEDAIRLTDLFFKIYRDKDIKDKIIYHRLKDMVIYGDAEFIKNHQQSLSMAILCCNSKKESKKLEPNQYQGLKLSAAISIFSFKKYKHLKMMDMYINFNYYIDLKHFENMTILYLDIDFYSSLVPRFPPNLKKLKVSFRQLFTNTTNWTLPSHLEDLELYTNRQVGLYYWKLNSALRNITVVGNFRGTVDLSPISHLHYYKIKDCRKLKIKNKPKIISKIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V138</accession>
    <name>A0A8J4V138_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Proline dehydrogenase</fullName>
            <ecNumber evidence="2 5">1.5.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_002020</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Converts proline to delta-1-pyrroline-5-carboxylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23784"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.5.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the proline oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.5.2" evidence="2 5"/>
    <dbReference type="EMBL" id="AJWJ01000053">
        <property type="protein sequence ID" value="KAF2076650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V138"/>
    <dbReference type="OrthoDB" id="5464at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004657">
        <property type="term" value="F:proline dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:L-proline catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002872">
        <property type="entry name" value="Proline_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015659">
        <property type="entry name" value="Proline_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13914:SF0">
        <property type="entry name" value="PROLINE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13914">
        <property type="entry name" value="PROLINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01619">
        <property type="entry name" value="Pro_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="FAD-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 5" id="KW-0642">Proline metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proline dehydrogenase" evidence="7">
        <location>
            <begin position="170"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="27"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2076650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="514" mass="58608" checksum="72375983E27791D2" modified="2022-05-25" version="1">MYRITSSSANIAYRSIYSINSIKYSKDTSPLSFSPPTSQSSPPRYYTTTVSSTTGKQHILQSTSAVIDQQTSIQNPKPSTPPSPASSPEQQKQNQEFEFVQRIYSTKTTSELLFNYSILKLCSFPLISDNCDKFIKLGEKLGVSGMMFWFIKRSFFKQFCAGETIEETTKSTKILNNRGIGAILDYSVEDLSGSPEGFDQVADQVIQTIKISAENPNLSFSCIKITGLSNPTLLEKMNKIVSSLSPNQFKESLADPLSLYLNHNNNIFDSQEKKDLESFFKRVERIFDECLKTRVPILLDAEQTWYQEAIHHLSLAYSMRYNKEKPLLYNTFQMYLQDSMETLNQHLEFSKQLGFKLGAKIVRGAYMVSERERAQEIHYPDPIQPDIQHTHNNYNNAIHLLLNTIKEQGKDSLGVMIASHNVESALIGVKLIQELGIDSTSTSIQFGQLFGMADYLTYDLVSQNQRVFKYVPYGPVKEVIPYLVRRMHENKGFIGSGSEKELRFLKKEIKRRLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V148</accession>
    <name>A0A8J4V148_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008581</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000543">
        <property type="protein sequence ID" value="KAF2070097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V148"/>
    <dbReference type="OrthoDB" id="20502at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040352">
        <property type="entry name" value="TMUB1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14557">
        <property type="entry name" value="PROTEIN C7ORF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14557:SF5">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="223"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="66"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="287" mass="32589" checksum="417198670302960D" modified="2022-05-25" version="1">MESPSVEGRHHHSSSFNGSNTMVDYNIDTTFLISFAAILLSFILWRSPLLSQLITSFSNFFNSNSTSNSSHSSTSTVPLSPESRSSTTSSTTTTNNINSSTSNETTQHHHQHNQPDEATPPVTIPFEETFSSNVQDLTKINFILSEQVYHRTFRSTSIIRVLDLKKYFFPNEYQDGKRIIFIYNGRALVDTEQLNPLTADQITNDPNYSTESTSAFTWMCTKTLYILTGLFLVILWSLKVTSPKLFNFFSTIMLLVFTLFWAFGFYSSLKTNHHNFATATAQHLHQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V199</accession>
    <name>A0A8J4V199_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Leucine-rich repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003375</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000105">
        <property type="protein sequence ID" value="KAF2075298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V199"/>
    <dbReference type="OrthoDB" id="18598at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034315">
        <property type="term" value="P:regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051279">
        <property type="entry name" value="PP1-Reg/Actin-Interact_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24112">
        <property type="entry name" value="LEUCINE-RICH REPEAT, ISOFORM F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24112:SF65">
        <property type="entry name" value="PROTEIN CARMIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="843"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="941"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="843"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="889"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="982"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1010"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1025"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1036"/>
            <end position="1053"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1053" mass="113675" checksum="FD0C3C4E9CD32529" modified="2022-05-25" version="1">MADEITQNDRKNISHILTQKNQESVLMSIAEKISKKKTKKPSKRIILVTKNRVLFFKPGVSKIKKETHLLDINEIRSTTSSEVIFITKSFSYGLITNKADEIINQIQTIFGQTFLGYPQDYYKFDVKPPSRVKDNVPKDLPCGGFVETYTSLCDYYSIPIRDDICWDMANIVSSKNIKTFNIGEIEQPISPGDIKALLSALKYNTYFKSLVFNGHPFGKDQFIILAELLKTNSTIEDLSLNNTGLKSDTLPIISTTLAANKNLALTSIDLSNNPFEDKGMVPFSAYVAASPRGIASLDVSNTGTGKVGIAALTNALKKNIKMTSTLQYLNLSNNKMEADGSAGLANFLANPNALRTLNISNTFPILDTIVGALVRGCLELRSLDLSDIRLTKKETAHLVRFIGASSTLKFLNLNNTKVPVENLKEIVVAIYSNIYLQDVNLDLKNNDLSIAGARMLSSVADKLNNVTILDLSENDFGDEGVSVICEGFTSNTCVKRLLLNGNFKTSKTKSRSASIESVITLLESECPIETLHITAGNSKSPLKNDILPLIYSLATNSSLLELDITGHQMGVKGAIALGKALQTNKTLHTLIWDENLTTLAGFSGFLVGLERNLTLKNMQTPLTDIIAAHRENPTKLQSVLKDIDNCINRNQSPSRAFEGTGGVGATNLSFLASGQQQSIEKLLNKIKSIGRKATDPDQLLVIGDAENTEKVIGGLHLIREAVHSQLEMELNAKLKDFVKVANDIIDAKKNEMTQQILESMSSTFKSMDPQTYKRLATGIQFGSKDVDEIQIHSTLVKGAGAELSSRANECFISALDIASDYTYEKISLGLEIVFKDLLEESLAENSNDSSPAPSKFVPSEKPTEKPPKESFLSPTKEQQPPVAATSPVSAPPTSAPPLAPRTGAAAPPQSPEVIPQPVFKPSGLKLAGANSAVAAAIARNIGGGAPPIRKPAAPMSEGSVPPSSTTPTPAPSGFKPKPVAPPRSAGTAPSSAAAPSKPIVAKKPSPPSANASGDISTAPDSNTEELVHVTKDRPQVAHKRKPPTRRPRPPTEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1M6</accession>
    <name>A0A8J4V1M6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_003103</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000093">
        <property type="protein sequence ID" value="KAF2075598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="18780at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051191">
        <property type="entry name" value="DCAF12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025139">
        <property type="entry name" value="DUF4062"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19860">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19860:SF40">
        <property type="entry name" value="WD40 REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13271">
        <property type="entry name" value="DUF4062"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="BRCT" evidence="4">
        <location>
            <begin position="82"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="1057"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="1099"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="1184"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="1268"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="1352"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="23"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2075598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1500" mass="170371" checksum="F5914B6E3AB051E4" modified="2022-05-25" version="1">MSLPNSSNNGSLVKDALNSNSDNSSTSGSTTTPTTPTAPTAPTNATATTGATIATASISTPKKSYLTPATITTTKEDKVELPAIPIFQSMTFFILGLGFTESLQLKNQIKKYGGDVAFQINKNVHYLLTNKTEIEKKSSKLQSAIKLKSIDIISIDFISFCIEKRYFSHQDLNTYRLFDRDENNSDASSSGNGSGGHGSDNSIIDIKSKLQKLSIKDTNSTRAKSTNTLNQLDLIREKKKTCKLFISSTFLDMEKERECVALYVLPQIKEYCAERNIEFSHVDMRWGISSLEDLKKKNTIGTCLQEVSTSHYFVSLIGDRYGWCQGDQYQESFELGKIYYPWIEQYQGCSVTELEVLQATKHKSSITQSSLFFFKSSAASAALDLPKEDQQCLEKLKALKASITEQHSAQLGKFNDLVELATGMFTSVAKLIDRDYPQVGNGNQFLFNVDKHLSYSQSLLRVPYVNNNAAIMQSLNNFVLSHGGSHVVSSQQPFIIAGVPGYGRSTLIANWISRLPSMLDTKKTLLINVYVGITPASRNRYEIQKQLCLIIRSHYQINVPLSESFESMSEEMNYWFDIATRDGNKLVWVIDGVDKIETSLNESLKDLLVSFIPSKYTPNVKIVLSCYADNLPALLSIYDKEQMQSTHVGTLKGFESESDIIQFSKDYLSLYSKEMDLVQIEILKNFLPACESPLFLSSVLGELVTVGNYANIYQKMATLLSLEFALRLYTVIIGRLEIDTNYPTGLVSNVIQLINLSENGLYEEEILGILGIQSSNGLFSALRSIFGHGNYFSIYSPYLLMAVRFTYHQGKQDYSVPSSHEVKLLSMMRDYFVKQPCSQRKVDYMVRVLKSLNDKTTLREFLLDFENFNLMSKSQDMGRINIINLWTFVGYKSKLEIYHLYKDNLVKYKSYFPPLEELATTLYNLGCLMDCLSLYDQAFELLELSQDYHIQLYGDVHASVCSDITRLAFVNIKRSKFDTAEYQAEKSLVVSQSIYGKDQIGTFEQLVLNGLVQKKKTNYSRALEYYNDAKQTLFTFLAYKSASATATSPLLYNAKVADLYNHLGDIYRKLAQFDTALDFYNKTLDIYTNLRKPQHVSFYPLFKNLGLIEKKLGNYSKAQEYYESSLEIISIHLGKDHFEYGLVLCDIADNKRKQEDYTAAHDLYNQSLSILITKLGSENSIEIAEIYNDLGLIEKKLGHYQKAIDFYKKAITIGEKTLGRLHQKVSFFNQNLADVYRKLGDYKTSEQYFSKCLSSTQQHLGNDHPEVADILVSMGSIYKKQSKYQLAEREYKRAIIIITKAFGADHYKCGVYTNLLADVYRKLNNYDLAKNFYNKALNIIEKSLGKQHSEYGEVLYSMGLLQMSMTNFDQAIQLIKEAITIIEKELGKEHAKIGIYLNSLTETRIAQEMANPHCSLSTNVVELDSIRAMQDKSFAILIKDLGEHHPEVADLYTNRGEFEFRWGSHSKSREYFEKALEIIKIAFDESHIKYQTISDRIRYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1U2</accession>
    <name>A0A8J4V1U2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TIGR01777 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002876</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000085">
        <property type="protein sequence ID" value="KAF2075793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1U2"/>
    <dbReference type="OrthoDB" id="276721at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013549">
        <property type="entry name" value="DUF1731"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010099">
        <property type="entry name" value="SDR39U1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01777">
        <property type="entry name" value="yfcH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11092:SF0">
        <property type="entry name" value="EPIMERASE FAMILY PROTEIN SDR39U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11092">
        <property type="entry name" value="SUGAR NUCLEOTIDE EPIMERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08338">
        <property type="entry name" value="DUF1731"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1731" evidence="2">
        <location>
            <begin position="272"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="323" mass="36038" checksum="B8659622F11BD344" modified="2022-05-25" version="1">MKKILISGSSGFIGSPLVEFLRSKIEQYEVYTLVRRPSTSRYEIEWNPERGQLDKDTLENLSPNVVIHLSGENIVGFWTEDKKRKILESRVKPTHLLCETLAGLKNPPQLFISASGCTIYGTNCSSPTDEFAPKGQGFLADVVEKWEKACEPLHERNRNIVKKEEATPSAGTNTPVATRIVHLRIGLVLHLSGAFLGTIYYPFLFGLGGIVGSGQQYIPWVSMRDTLSIIEFIITNNQVNGPVNVSSINPSTNAEFTKTLGSVLNRPTICWVPEFLINMVMGKEMAGETMLNSQNIIPTKLVNYGFKFTDTNLYETLYTIVNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1X6</accession>
    <name>A0A8J4V1X6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Hydroxysteroid dehydrogenase-like protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_007773</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000433">
        <property type="protein sequence ID" value="KAF2070907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1X6"/>
    <dbReference type="OrthoDB" id="5327538at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09762">
        <property type="entry name" value="HSDL2_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000301">
        <property type="entry name" value="Hydroxysteroid dehydrogenase like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051935">
        <property type="entry name" value="HSDL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006133">
        <property type="entry name" value="PRK08278.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42808">
        <property type="entry name" value="HYDROXYSTEROID DEHYDROGENASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42808:SF3">
        <property type="entry name" value="HYDROXYSTEROID DEHYDROGENASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2070907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="280" mass="30872" checksum="5A2C4B6D4ECE4D67" modified="2022-05-25" version="1">MFKGQTLFITGASRGIGREIAIEFAKLGGNVAIAAKTSDPHPKLQGTIYSVKKEIEDLGGKCLACVCDIRFEDQIEAAVQKTVETFGGIDILINNASAISLTNTLDTSSKRFDLMMGINSRGTFLTSQKCIPHLLKSKFTPHVINISPPLNMNKKWFAGHSAYTMAKYGMSMCVLGMAEEYKDKISFNALWPRTAIFTAAMEMLAGSDVANECRSCKIMSDSVLWILKQPINVTGNFFIDEYCVTQAGVTDLDQYAFKKGEKLMNDFFLDGDIDYTTAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2I5</accession>
    <name>A0A8J4V2I5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_007119</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000362">
        <property type="protein sequence ID" value="KAF2071567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2I5"/>
    <dbReference type="OrthoDB" id="13950at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05374">
        <property type="entry name" value="17beta-HSD-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051911">
        <property type="entry name" value="SDR_oxidoreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43976">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43976:SF16">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2071567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="290" mass="31886" checksum="20F681D1BD1206C4" modified="2022-05-25" version="1">MSDTQANDNRVWFVTGASKGIGLSLVKRLVKNGFRVAATSRSTEQLIKNLGELYNTDQVLALSVDLANEESVKQAVSDTLGKFSTIDVVVNNAGYGFFSPIEAISDKEARDLFDINFFGPLNIMRNTLPHLRTNKSSEYGPRIYNISSVGGFFGNFPGVGLYCASKFALDGLTQSANAELAEFGIKVTSVLPGYFRTEFLQSTSVSVSKNFIPEYETANKVTDLHINQVNNNQSGDPDKLADLLINISREKNPPLTLPVGPDAYEYFGKRIEAIQKDLEAFKDQTTKTNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2N2</accession>
    <name>A0A8J4V2N2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TerD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009743</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000793">
        <property type="protein sequence ID" value="KAF2068939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2N2"/>
    <dbReference type="OrthoDB" id="5240116at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06974">
        <property type="entry name" value="TerD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.30">
        <property type="entry name" value="sav2460 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051324">
        <property type="entry name" value="Stress/Tellurium_Resist"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003325">
        <property type="entry name" value="TerD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32097">
        <property type="entry name" value="CAMP-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32097:SF17">
        <property type="entry name" value="CAMP-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02342">
        <property type="entry name" value="TerD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TerD" evidence="2">
        <location>
            <begin position="93"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="283" mass="30175" checksum="46A2DE2C2E293AAD" modified="2022-05-25" version="1">MYNPPPPNNGSGSSQPTYYRQPSSTPGVSNPAPNAGFRPPAGGAPPTAPGGYGQPPMPPAPSSTPNNYPAPPPPIAPGQYGAPPAQAPQGPGISLYKDQQISLSKEDPYLRKLTAGLGWDVNIQNPGSPFDLDAVVFMLGANGMVRASTDFIFYNNKQSRDGAIYHHGDNLTGQGEGDDEVVSINLQAVSPDVTRLVFAVSIHQADQRRQNFSMVPRAFIRIANQETSRNICRYDLTNEGGYNTALIVGEVYREGPEWKFIAVGKSFPGGLGYLCQIFGVNVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3F7</accession>
    <name>A0A8J4V3F7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CENP-T/Histone H4 histone fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_006170</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the histone H4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000272">
        <property type="protein sequence ID" value="KAF2072527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3F7"/>
    <dbReference type="OrthoDB" id="30042at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22912">
        <property type="entry name" value="HFD_H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035425">
        <property type="entry name" value="CENP-T/H4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001951">
        <property type="entry name" value="Histone_H4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10484">
        <property type="entry name" value="HISTONE H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15511">
        <property type="entry name" value="CENP-T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00417">
        <property type="entry name" value="H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CENP-T/Histone H4 histone fold" evidence="9">
        <location>
            <begin position="16"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF15511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2072527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="78" mass="8567" checksum="0D68CFCC51105DA2" modified="2022-05-25" version="1">MSSVGGRGKSGGKVGGGGVKRISMPIYEETRNVLRTFLNNVIRDSVAYTEHANRRTVTAMDIVYALKRQGRTLYGFNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V465</accession>
    <name>A0A8J4V465_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_008219</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="AJWJ01000489">
        <property type="protein sequence ID" value="KAF2070454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V465"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17967">
        <property type="entry name" value="DEADc_DDX3_DDX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000397">
        <property type="entry name" value="Probable ATP-dependent RNA helicase DDX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044763">
        <property type="entry name" value="Ded1/Dbp1_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="13">
        <location>
            <begin position="259"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="290"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="506"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="659"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="8">
        <location>
            <begin position="259"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="70"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="166"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="671"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2070454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="716" mass="80024" checksum="EBFC6C1FEB46E4E1" modified="2022-05-25" version="1">MPIGNKNENDGSPLNNLSGLTISDKKSQTTSTTSTTNEPSVYVPPSRRNRMSSSTGSNSGSQPQSPAPSNDRDSRERQENRDPRDFRDNRDPRDFRDNRDNNNNTDSDRRDNYYDNRGGDNYNRGGYNNRQDGGYNRNSPQQYGGGAPRGSDRWGDNNRGGGGGYNNYSPNPSFGRSSFNRGGGGGYNKGNRYDRYFEKWSSFKDSRNHPDMPREVFNLDEEKAEEIFKSNSNHGIDFNSYDDDDISIETSEHLCAPINSFSDVDLGDILFANIKHAKYTKPTPVQKSALPIISKGRDLMACAQTGSGKTAAFLFPIISGILLDGCPEQPSTSYRVGVPRPAQPRALVLAPTRELAQQIYDEANKFSYASPVSSVVIYGGADVSHQIGELEKGVDILVATTGRLVDLLDRGRVSLSKVKYLVLDEADRMLDMGFEPQIRAILGDYDMPGNRDRQTLMFSATFPKQIQALATDFLNNYIYLKVGVVGTTQNITQRIEYVAEDEKNSYLLDYLSNLKSDQGLTLVFVETKRACDTLTYYLNKSQFPTTCIHGDLSQAERENALQSFRSFQTPFLVATDIASRGLHIPKVMYVINYDLPTDVDVYVHRIGRTGRAGKKGFAISFFNEKNKPVAADLLKLFKQSNQETPEWFEKMVHSFKSSKGPMNKKYDNKGYHNNNNYYSNNNRRGSGGDDRRGGGGGSGDDYSMHHHFFSKGGSYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4R4</accession>
    <name>A0A8J4V4R4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_004875</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000181">
        <property type="protein sequence ID" value="KAF2073817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4R4"/>
    <dbReference type="OrthoDB" id="19711at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042627">
        <property type="entry name" value="FBXW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44436">
        <property type="entry name" value="F-BOX/WD REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44436:SF1">
        <property type="entry name" value="F-BOX_WD REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="6">
        <location>
            <begin position="106"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="202"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="242"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="284"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="411"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="451"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="492"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="331"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2073817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="565" mass="65157" checksum="F0F063349BB598BD" modified="2022-05-25" version="1">MLGPYNSFTQNVSNYHFDSFPYENSYEEDEYEDHYKEMAFYQKWGSKYSNQVKELKKTMSSPSMLTSSGSNLFSSSSNSTSSKRQKFKKILKNFILPNRFNPLKRETFISILPVEIVVEIVGNLSYKDVLNVSLVCTEWFYLAQEEILWRSVYQTYFVYHPNRELALSKPQNHSDQHWREIFKEHHFREAKWSGDRYQESHLIGHTGTVWALHMDAERVYTGSFDKTAKVWDINTKKCKYTLSGHYYPIQCLDVIDNTMITGSLDNSIRVWDLEEGKSKGILTTRAHNFDVFCLQSQDNLIFSGSSDSTVKIWNISNILESSQTPLDELLENDLLQEEEQRRQQYQQLQQEHQNNQQQFPDDVSSLMDPFDVGSMISEEGGNGTSSHINIVSSNDRTSRRYLNHNMQSVETFKHTSCVTCLQVKDNILMSGGSDKVVRVWDLNTSQSIDVLEGHHEGIRALQFNGNVLVTGSDDTTVKIWDLRHKNHCVSTLRGHTGAIRCLQWEGATLITGSNDQTIRYWNMNSGSQSSRELFSFSSSISCLRFTENVLMCGLSDSKVQITKFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4Z4</accession>
    <name>A0A8J4V4Z4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007337</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000385">
        <property type="protein sequence ID" value="KAF2071339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4Z4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051860">
        <property type="entry name" value="Plasmodium_CSP_Invasion"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44826">
        <property type="entry name" value="SPORE COAT PROTEIN SP85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44826:SF3">
        <property type="entry name" value="SPORE COAT PROTEIN SP85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="479"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="574"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1039"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="482"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="507"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="574"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="645"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="716"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="740"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1041"/>
            <end position="1058"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1058" mass="117691" checksum="B8776ED433991527" modified="2022-05-25" version="1">MAQSFSFKLRRINTGIGSNITKSLLLLLLFLNLFAVQSKQLFQSVFSDFNPVEYDSSVPFSFQNEILFLLNDNAYNISGSNNQLEFYYLSNDATDLSVTILDSFDEKYTLETYYETNVDIQDETWNLIVLPLPNVNIKQISVNNNQQIDGNDILIHAPNIKAASMTPTLNIMKYYHSKNSGRKFVKMQEDDFIPADSKLGDLCLRKSNGASNPICWYQYEPKSHLFTFKFYKPIKNFLFLFGSMVGRLDNFVVANDENSVQYDIKNHWKRLDSSNLGLWYSNKSTSYISGQFKVGPTQKFHEKDIVFTIMILDSDPKDFTKCMKYHQPFIISETFDIPSISAPKPAKLPNGKKCSLLVSSKKSSGGPDFDVWDIYLLNKSPSSHQFVNITLVPKIISQKGLATIEKDHFDFPHGVHPGEFYFFQVTTTKSNTPPLLLSSSCRIENYELLEEAPIDLNDYNNTEVQSIIFNQTVDKAVKKSPKSTSTPIIKPSIQPTSKPKGKPQSSPTQTTKPTTKPTTKPTTKPTTKPTTQPTVKPTVKPTVKPTVKPTVKPTVKPTVKPTVKPTVKPTIKPTVKPTVKPTTTPTIKPTVKPTTTPTIKPTIKPTTKPTTTPTIKPTIKPTEKPTTTPTIKPTIKPTIPPTPTPINSSSSVGSQSSQSSNENGSNGFSSSQSNQTSHEPTSGSTSVSTTGKGTSQETSQETSQETSSKDRSHELTTFGSTSGSITISGASTTGTGGQGSTSSQGSTSGSTSISSSTTISPTLSPTPSQSPTPDPFPLVDISSEDELTSKPFYATDVYGNAFPFLKYYVYESRSTPRLTRFTNINGAIDELYLSRAFSLEKARYMVFFETPLGYQPLPKFPPVDFKPVQGVVFEKIDPLQKDCYVFGYVFKDRFHNYILDAFDVDVNDTTGIIMQPIYLVDDKMNVINQTRTNEKGQYIFKVDKLYPKVSVVFPLSNYLTVLYHESSEQGGEEEYHPDPPGESIIIKDNSQIIVFKGVQSMQSQTFSIDSLVNRFGPNIALQLDNSLNKLFLSKKRLRSHSNPINNNNNKKNSIKINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5L6</accession>
    <name>A0A8J4V5L6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">BEACH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_000375</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000007">
        <property type="protein sequence ID" value="KAF2078283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5L6"/>
    <dbReference type="OrthoDB" id="26681at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06071">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01201">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1540.10:FF:000001">
        <property type="entry name" value="neurobeachin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1540.10">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036372">
        <property type="entry name" value="BEACH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050865">
        <property type="entry name" value="BEACH_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057496">
        <property type="entry name" value="FAN-like_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023362">
        <property type="entry name" value="PH-BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743">
        <property type="entry name" value="BEIGE/BEACH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743:SF123">
        <property type="entry name" value="PROTEIN FAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02138">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14844">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25400">
        <property type="entry name" value="PH_FAN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM01026">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81837">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51783">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BEACH-type PH" evidence="7">
        <location>
            <begin position="295"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="BEACH" evidence="6">
        <location>
            <begin position="395"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="812"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="897"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="947"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="87"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="141"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="678"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="698"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="87"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="141"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2078283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1113" mass="125794" checksum="D174AABF42C61409" modified="2022-05-25" version="1">MFTVNRTKLRFSLILMEEGEYYFDDYNATFYPPADTEEESWAKRVAGRVLICSNSIFFEPDDNKLPIMRMPFKEVLGVGRWLNSASSAPTLPSSSMSQSLPPPPSSREEPPKLPPKTAVSSTSGSALTRFFSTIPFARSVSTSTTSSTLPNPTTSPISSTPTLTNTSPIPSKQNINNNSSNNNNNNNNNLNPFDRRSIPTVTPSYIASKVDVFYIKTTQIIEMKEGNKNAPYVFKTCNLDLKFSLSYTALPPILETIKDFLQYSKKEKTQHENIIKSLIEDRENKHQFDLSLLVDLNEKSILELKCSKISPLVENPGRLLLTNARLYFQPMNNIEGKIFSHHNLESITRVQKRRHSLREIGLELFFDDNSSLFFKFGHTYDRDRVYNLLVQHLCPNIVNVHEQKNYLLKWQNGIISNYDYLIYLNNLAGRTYNDLTQYPVFPWIIQDYTSSELDLTKPETYRDLSKPIGALNPARLATFQQRYHHIPDGEPKFLYGTHYSAPAYVLYYLVRQAPEYMLRLQNGSFDQPSRMFHSIQETWNSVMNNSGDVKELIPEFYKPVDAFSANYCGEFLVNTEQLDLGVRDHNEIINDVVLPPWANNSPKEFVTTLQKALESDYVSSNLHHWIDLIFGHKQKGTEAVKANNLFYHLTYEGAVDIESITDPFKRQVLESQINEFGQTPRQLFTTPHPQRLPQSSRNQNLSIELNDLEENINHLYNTLNNSDELINSNNNTSNNNTSFDLYSFNNNNSNSNTSFDGFSSNSNLKKSSSVNNLSFDLNSSMDYSSNNNNNSFLNDDDRNWGSLANFTLNQVIKGHKDRVTALCLSSLNPDILYSVSKDSLLKIYSLREGKQNRSLNLCGLALSSIQLSRDEKYIIIGSWDDNIYVYSVQNGSISYSLAGHSDAVSCLKLHKNMLVSGSWDSSVKVWRVERSPNSGAITVDLRPMADFVESESEITCLDISPDGMTCVAGSKDGHIYFYDLVSLQMIRKSSLYYHKLTCIKYTPDGSRIVTSCVDGSLKLIGNEGSEIFGFQCDDKVYCLDTDGSTLVVGTRSGLRLWSLTTGTEIKDTASSFLSQSSQDSIRSLCVTMTNGRPTLLTGTLNGSISIWSQSQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5Z2</accession>
    <name>A0A8J4V5Z2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PSP proline-rich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000083</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000002">
        <property type="protein sequence ID" value="KAF2078583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5Z2"/>
    <dbReference type="OrthoDB" id="10260794at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007180">
        <property type="entry name" value="DUF382"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006568">
        <property type="entry name" value="PSP_pro-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052584">
        <property type="entry name" value="U2_snRNP_Complex_Component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12785">
        <property type="entry name" value="SPLICING FACTOR 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12785:SF6">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04037">
        <property type="entry name" value="DUF382"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04046">
        <property type="entry name" value="PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00581">
        <property type="entry name" value="PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PSP proline-rich" evidence="2">
        <location>
            <begin position="316"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="419"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="573"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="456"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="601"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="615" mass="70184" checksum="2B9213B61EE1E7ED" modified="2022-05-25" version="1">MNGIESINGNGTLPHNPTVGGGGDKKKQKQQKKKEQKKRQKQKKQQEKSVTVATATENNNIDQEQLEKQEISNNINAYILKKADNEKQQQDESDDFDFQLAENDPTFEMFNKLIKHFDGKDDENNDNQADDGQENQQEEENKENEEQDKDQENEDQENEDDKKMSNKERKRQRRLHLPILKQLVDRPDVVELHDVNSPNPGFLISLKSYKNSIAVPAHWCQKKKYLQGKRGFVKPPFELPSFIAATGISKIREAILEKEKEKKSKQKQRERVQPKMNRMNIDYQVLRDAFFIHQTKPKLGIQGDLYYEGKEFEVSITAKKPGQLSDELRKALGMADGYPPPWLIYMQSFGPPPSYPNLKIPGVNAPIPEGAKYGFHPGGWGKPILNEYGKPMFEQPTPSIVEAAEAVPIVREYWGELLPEEEEEEEEEEEEQEGQQEDGGEQIEGEEEQQEESEQDYINQGISSVPSGLETPDVVDIKKSRYNISATGLGHAPDQPKQLYHVIEQQQRSMGGGLMESNYKYNLPGVIKTANSGRGGAGIIKSQKSGPVEITLNPSEIEGEIDEDLLRKKYEQAVSNDAARNAKPREDYSDIIEEQTKKRKNQLEKKDDKSKKFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V656</accession>
    <name>A0A8J4V656_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_003189</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000097">
        <property type="protein sequence ID" value="KAF2075497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V656"/>
    <dbReference type="OrthoDB" id="6375174at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14531">
        <property type="entry name" value="PFA-DSP_Oca1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000035">
        <property type="entry name" value="Tyrosine phosphatase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020428">
        <property type="entry name" value="PFA-DSPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004861">
        <property type="entry name" value="Siw14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF8">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE OCA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03162">
        <property type="entry name" value="Y_phosphatase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01911">
        <property type="entry name" value="PFDSPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="8">
        <location>
            <begin position="9"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2075497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="163" mass="18825" checksum="1D81A59352C2C130" modified="2022-05-25" version="1">MAHYIPPLNFGMVGDDLYRSGQPNELNFPFLEKLQLKKIIFLAPDEPSPQFQNFVEDQDIELIHLGMDTHQNPWNPISEEIVISALKIILNPDNYPLHIMCNLGRHRTGTVVGCLRKLQRWNLTSIFEEYRRFAGSKVRLLNEQFIELFDTDLVSTDLVSNGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V683</accession>
    <name>A0A8J4V683_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000094">
        <property type="protein sequence ID" value="KAF2075572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V683"/>
    <dbReference type="OrthoDB" id="21077at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044613">
        <property type="term" value="C:nuclear pore central transport channel"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006607">
        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036228">
        <property type="term" value="P:protein localization to nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025574">
        <property type="entry name" value="Nucleoporin_FG_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024864">
        <property type="entry name" value="Nup54/Nup57/Nup44"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13000:SF1">
        <property type="entry name" value="NUCLEOPORIN NUP54 ALPHA-HELICAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13000">
        <property type="entry name" value="NUCLEOPORIN P54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13634">
        <property type="entry name" value="Nucleoporin_FG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="461"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="461"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="578"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="593"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="637" mass="65900" checksum="C718C51646C2B393" modified="2022-05-25" version="1">MFQATPGSSLFGSQAAPSTGLFNTTAGSTAGGLFGNVGATPSTPISTGGGGLFGNNPTTPSTPVTTGGGLFGNNPATPATPLTSGGLFGNTAQTPSIGGGGGLFGNTQTPSTGGGLFGNTATAAPSTGGGLFGNLTQQTPSTGGGLFGNTGTAAPSTGGGLFGNTSTAAPSTGGGLFGNTTTAAPSTGGGLFGNSLTTGTSGGMFGASSLGPQQNFGNNSLFPSQPQQQLSLQQQPAQPQITENTKFSELNPLLKQELQKFNKTIINNNSLMKSFSPNFDKLDNISTEIDQIVSNIDSLFNNFDIHIQSFKYLEESLSIPLNSVNEQYHLLSTNILPPFSQPIPSREMVMIVDGLIEKVERIRQTLNYVAPVQRNNHSNMSFQESFVTTLQSNQNILKNEAVLVDHLVSQLEKAKRHYLHYKKQYYNDNFNPFIKNHIRVQQQQQLESSIYNSNNNSQYNNSLYNSTSSPLSSQTSVFGTSLNRNPSTPSTPTPTNQSSFGSSGYYTPSSSLTSTPLTTTPTTTAPSLFGNTSLTPTTPVTATNTGSSSLFPLSTGLGSQASSLGSQGNSLGSGLGSTSGSTSGSGLFASSTAVPQTPHLSSQGSFLSGTPSFSISDNTRSLSASTQATNKQPRGNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6H1</accession>
    <name>A0A8J4V6H1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002861</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000084">
        <property type="protein sequence ID" value="KAF2075827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6H1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="278" mass="31929" checksum="C141F396D4FEA9D2" modified="2022-05-25" version="1">MEKRNGLMDHLIVASTRKRVSTDQQITASQPNINTTTSSMLPVVGRSSKKQKVIENQSLPDINFSNNNNNEMDESIVDSTLSQPQASSGYNFFSSQNTTFGGGFSMDPFSSQSNIPFQDVYQQHSNSNDNEFIVSENEKESKRTKKKERKERKERKEKKEKDKKEKKERKENDKKDKKDKKKEKKIESQEQPQEKEQQDQEQDMVMQENIPTLPITSSQTYNFFSTPFSFNQENINSTSGSSSLAWDLPSFTGEGGNPAVIDPNEHSYSFFSSFYNQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V780</accession>
    <name>A0A8J4V780_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phosphatidylinositol N-acetylglucosaminyltransferase subunit C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_005014</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000190">
        <property type="protein sequence ID" value="KAF2073664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V780"/>
    <dbReference type="OrthoDB" id="196709at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000506">
        <property type="term" value="C:glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009450">
        <property type="entry name" value="Plno_GlcNAc_GPI2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12982">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN, CLASS C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12982:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06432">
        <property type="entry name" value="GPI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="318"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="340"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="369"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="393"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="48"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="78"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2073664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="437" mass="47802" checksum="2BE7D5505C773714" modified="2022-05-25" version="1">MENQKHSGASSPSTTQLGGSTQIPSISNNSSSTELDRCHYCGNELTTNNKMIHNGSSSTPPTTTGNLIHCTNVQTPLSSSSPKIISTTTTTNINTIDTYSSSSSSSSSSSSSSSSSLTSPISNTITNSNSNNNSNTETTPIITTPSTSTTSTTLKWRKNLYEKQSFADNYTDETFLIGLVQNANFMKYDFWTVVLDSFTVSQQITSVILFVIIFFHSLKYTLSLPFLVGMAGIFLVLGYIVLIIIDPTSNFNTIKDSILHVVLLFGTVYGLSPVLRTLTNSFSDDTIWALTFILLLAHLFFHDYGYTNNETVKFSAPISLNAAIFASVLLGSRLASNTHVFMLITYAIQTFALFPILRHHLKRHSMELHAGLTVILCVTCSALLLGMSTLLALIYVGIIITITFVCPIWLIWIQKYKNEINGPWDEASVISQSGGEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7A6</accession>
    <name>A0A8J4V7A6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">[histone H3]-lysine(4) N-trimethyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.354</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_004923</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60260"/>
            <dbReference type="Rhea" id="RHEA-COMP:15537"/>
            <dbReference type="Rhea" id="RHEA-COMP:15547"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.354"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6),N(6)-dimethyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60272"/>
            <dbReference type="Rhea" id="RHEA-COMP:15537"/>
            <dbReference type="Rhea" id="RHEA-COMP:15540"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(6)-methyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-methionine = N(6),N(6)-dimethyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60268"/>
            <dbReference type="Rhea" id="RHEA-COMP:15540"/>
            <dbReference type="Rhea" id="RHEA-COMP:15543"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.354" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000184">
        <property type="protein sequence ID" value="KAF2073754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:histone H3K4 trimethyltransferase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Set1-like"/>
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        <property type="entry name" value="SET_dom_sf"/>
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    <dbReference type="PANTHER" id="PTHR45814">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45814:SF2">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
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        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00856">
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    </dbReference>
    <dbReference type="SMART" id="SM00360">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RRM" evidence="14">
        <location>
            <begin position="408"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="15">
        <location>
            <begin position="1294"/>
            <end position="1411"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="16">
        <location>
            <begin position="1417"/>
            <end position="1433"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="612"/>
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    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="918"/>
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            <begin position="1163"/>
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        <location>
            <begin position="836"/>
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            <begin position="10"/>
            <end position="40"/>
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            <begin position="41"/>
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            <begin position="129"/>
            <end position="141"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="13">
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            <begin position="162"/>
            <end position="187"/>
        </location>
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            <begin position="188"/>
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        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="13">
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            <begin position="211"/>
            <end position="232"/>
        </location>
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            <begin position="233"/>
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            <begin position="1018"/>
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            <begin position="1041"/>
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            <begin position="1065"/>
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            <end position="1208"/>
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            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012182"/>
        </source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF2073754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1433" mass="160394" checksum="F33906278E03C3C3" modified="2022-05-25" version="1">MEATVAPSLKSINHQHTLASSTTTTNKAPVQSNADDTTIVNNNHIRNSSGGSSSASKSNPDIDIDSNSSSKTSNNNNNNNNSNDIMDKGDSSGNSFSNDSVHHQHSSEQFDEWNIKRVSNYDQFSSEDEQFKVKNSRREGSGGKYKSSKSGGGRSRSKSRSDSSGSDSSNSSRSRSGSSSRSSSRSRSNSRNKRRHYKRRSSGKYDDRSRSSSRRRHHRRHSNSGSTKKRTRSPTNTSRSRSTSPSSSKNGGYKYKSSKSSSSSRYKTSSGSSKQQQQQPSTTNALITNSNVVNLPKEFKSKPRFAHESNHIFPIRSRGGNWRLMSQFSTDIDESGDIISSTNSSSSFKGHSVNGFTTPSDTIESVFGTHLIDPRDYNTVYNTPTPSLELLIPHFNNDRNSQTVHLPRTIHLSKLDTAVNEKQLQDIFNRYGAVERVKIFYHPKTNQSLGVATIKFYDSVAALTSIRLFSQSPMIMNRVVKINLDPRGSLSQSIFQDILNSAMPKVRATERLIKDFGISEYGNITAEAAPGDWMSQFGIKATNNINIFDYDNTSYSSSSPATSTPSTMISFKQQQQLDSDEYDPNHPTDPLSPNYELYPHFIFSKSIVQPPPPPLPIHASHYHHQQYHHQQHLHHHHLSPLSNRTTSSSLEVSNSSGNLPINGNSIHIHSTPTSNTPNRASATPSSTAASALNSTPNRASATPTTTNTNSNNTSTPTTSTTATTTAATTTTATPTKTFKSKFSTLQPSPYSVQIPKTPNMDLNMSAVEKKRITNKLLRHFKIFDPINVYSDEQFWYIDFRSSDSRDRAISVLNGSYLEGYNTKLNVNSKTTDAINIELVNIQLKEQQQQQDDKQQQKQNQQQQFNLFENENYLLEICTMLVSTELLSISAKDAQKHIVEPEIFQSVKSAEQKHQEPVVVPLSNGGNLSSTSQSSTSTKPTSNTSNSSSSNISNNNEANHSITTSHSSKSKLDIHSIPSFKKAKPKSESNQTKKKKHYFSDNESDEEVVVHKKKTPTPNNSNNNVNNVNGRKTSRNNNAYYSEDEDEDDEEQDEQDNHSVTGTDLSGDEDYLDNEVDNKKSNRKSTTSGKMPRKSHHHHHKEQQQQQQQSKKQTRSIKNISPPHFDEDEDDEDAYYMQEIMKDSTINQLLSTKSEEDFDQIIEHDDDDDDDYYDNHSDSDNIHQQKQKQRSKDAFHEEEEEEEEEEEEIVYATNSARTDGITRVLQHTEEKKRQMNALLTTKARGASSAAASGTTTSASADFDIASSKSARSSRYDNRLAGDHLLTASSLKLKTKRIKYQRSAIHDWGLFAMEPIAAKDMVIEYIGEVIRQKVADEREKRYVKKGIGSSYLFRIDDDTIIDATLKGNLARFINHCCDPNCIAKILNIGTQKKIIIYAKKDISVGEEITYDYKFPLEDVKIPCLCKSPKCRGTLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7I7</accession>
    <name>A0A8J4V7I7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fungal lipase-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001844</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000046">
        <property type="protein sequence ID" value="KAF2076877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7I7"/>
    <dbReference type="OrthoDB" id="345705at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051218">
        <property type="entry name" value="Sec_MonoDiacylglyc_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45856">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45856:SF20">
        <property type="entry name" value="FUNGAL LIPASE-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035327941" description="Fungal lipase-type domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Fungal lipase-type" evidence="2">
        <location>
            <begin position="96"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="293" mass="33029" checksum="0A1245A5A0BB4A83" modified="2022-05-25" version="1">MKILFLALVALQLLLLCNNGFVQSNSYPDINYDPNTAESFLMYSYAAYCEGPDISNWTCPTCSYKTMNDFKIVNLIYNATTDTQAYVGYYGNNVLVVFRGSMDVYSWITNLQFLYDAYPFCTDCYVHSGFHKAWLSVRSEIEASVANSMKLCGPKCGLIVTGHSLGGALATLAMAELIQIYKNIPTFIYTFGSPRVGNDNFASYYNSIQPNNYRTVNQHDLVPHVPTEVMQYHHVPTEVWYTSNGTYQECNDSGEDPSCSDSITNPATLSIYDHLHYFNIHCCCRDFNGSSEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7V4</accession>
    <name>A0A8J4V7V4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001513</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000036">
        <property type="protein sequence ID" value="KAF2077192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7V4"/>
    <dbReference type="OrthoDB" id="17418at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="72" mass="8639" checksum="360825B4FEAFB82C" modified="2022-05-25" version="1">MNTIATPTVQQLVQAYRQIIRKANKELKYTNFEYFRFRVKSSFKEPVETDYIKTRKYQDALYLIDNNLGNVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VB33</accession>
    <name>A0A8J4VB33_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000910</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000019">
        <property type="protein sequence ID" value="KAF2077789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VB33"/>
    <dbReference type="OrthoDB" id="21365at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22063">
        <property type="entry name" value="WH2_Actobindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00246">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WH2" evidence="2">
        <location>
            <begin position="3"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="353"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="403"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="440" mass="44712" checksum="B622A6C773E936EB" modified="2022-05-25" version="1">MGDRSGLLSQIQQGKKLKKAETNDRSSPLVAGSSSSSASSASSASTASRGGLFPGGVPSLPSRQPQQPPPPTSPHRPPPPPTSQPTPSSRPAPPAAPPHNRNSVSLPSHPAPLPPSPNRNSIIQQQHPTPPPPIPVHKPQPPQIISRNPPPPPIQHTSSRNSIYNMQQDSNDLYDGSDRWSFRSINELPPPPNFRGAIKNYPSGKNTGTTIKLPVVNKAPGVPNHSAPPIPPSSTTPPRPVSQINRPISTPPVNKPVPPPHANGPPPVPPHHANGAPPVPPHTNGPRPSAPLPGGPRPPPHTPAGGKMPARPPPPQAPGGGPPQQPPIPGGPRPPMNGGGPPPPGGPAGGKMPPRPPPGGARPTPPPPGATRPGPPPVPGHPSGSPGGPPPKPVRPPTTSMGGPPPPPGPRPVNGAPPPKPDRPMPPGGASRPPPALPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJ64</accession>
    <name>A0A8J4PJ64_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_010443</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001116">
        <property type="protein sequence ID" value="KAF2068232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJ64"/>
    <dbReference type="OrthoDB" id="538632at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF205">
        <property type="entry name" value="ABC TRANSPORTER A FAMILY MEMBER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="55"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2068232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="252" mass="28838" checksum="1207E00F95A86601" modified="2022-05-25" version="1">MGGDGHSVLWQSEGRNYYWSNFLQDSPSMPMFSAFKTLNILFFSGFIYLFLARNLHFSYSNFTIKGFKKSKILQGLNLSLEKENILCLQGPNCTGKSTTIKCITGDINSDKGNILLNGFNVEKQIHTIRTQLGYLPQSDVLWENLTAKEHLNIYCWLRYNSFDTEKEIECILDQVRLSNVSNNRVLTFSGGMKRRLSIGLTLIGAPKIMVLDEPTSGIDISNKLMIYKLIKDLFLIYINPKTFSVFLFLFSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PK67</accession>
    <name>A0A8J4PK67_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010393</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001081">
        <property type="protein sequence ID" value="KAF2068283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PK67"/>
    <dbReference type="OrthoDB" id="444265at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050923">
        <property type="entry name" value="Cell_Proc_Reg/RNA_Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23308">
        <property type="entry name" value="NUCLEAR INHIBITOR OF PROTEIN PHOSPHATASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="139"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="240" mass="28325" checksum="FF1DD075FAA9687B" modified="2022-05-25" version="1" fragment="single">SRERDERLQREDEYKQKRELQRISDSNKRDRGGSNKTSTNYDQQYGKKNNNNNNEEEEEEEEDPEEKRKRKYLKEKRDFKPSGALAKEDPSSTADGKEKIIMKFHEPNESRLPTEKWVLYPFKEKESLEPYFLHRKKSFLFGRNREIVDIPTDHPSCSSQHAVIVYRQIKIEKDNGSIGKIVRPYLIDLESTNGTIHNGKKIDTAKFYELRSKDVIKFGQSTREFVLLREDSIESDDSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PK91</accession>
    <name>A0A8J4PK91_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_010404</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DOCK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001090">
        <property type="protein sequence ID" value="KAF2068270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PK91"/>
    <dbReference type="OrthoDB" id="18896at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043161">
        <property type="entry name" value="DOCK_C_lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027357">
        <property type="entry name" value="DOCKER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653:SF8">
        <property type="entry name" value="DOCK FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51651">
        <property type="entry name" value="DOCKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="366"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="DOCKER" evidence="4">
        <location>
            <begin position="478"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="92"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="754"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="92"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="756"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="778" mass="89294" checksum="1EA2E819733C34E7" modified="2022-05-25" version="1" fragment="single">MIGITLDRDGTPTKDKEYGHISYPDSPPNTPIKTIYDFNNSSNSNSNSNNNNNNNNNNSKHLYHNSSIKDDEISDIAIQNRLFVNPFQRSRSLTISGSPTHKTSLTTNNHDNSNNNNNQEDEIDYSSSKQMIYATDDNIVDLKSSSSSSLCSTLSPSRLLYMDTTLSGSCGGSGNCTPLQLTPISSPVQEHRIAIIANNSTNTLSPTHFSLHHKRSKTHFVTSSSNSPLFNSIAPQVVLNTPSTPITPQTTSTKVFSPSRRRATCANLHNKLLSVYSPIKSPPLIDDNSNSSNISNNVESSTASPLILSNNNNETSSTTTTTITSNSNIDNKFKYLFHQNSKLESKYHVYDTIFSEYKESKRIYQEIGLSNLNVNLLIYIFQFLNVEEICQLGKTCRWWYTVSEHNTLWIMKFNKRFTRPQTIIERFSLSESWKELYIQHHFLDTSYMEFERLLSLFPVTVEYEHDITILLQKVLNYLKHTECISEYISKLEYLKNIHITFGNKIEAANCYIRHSELISWDANQMFPQENGYPIECHSDRKERLLKSALLLFIEGKYWERGLIIIKQLRKKYKKELVKYRNRRLSSSISIDQDLNNLNSNITLTNPNSLIVLKLLELSKLEQQCYNSIEGKERFFEEYFFVEFRGKGFPKSVEGKDFVFRGSELEKLGSFVTRLKNRYPGAQVVPKTFDIDSNPGQYIHIRPCKPVSLPKEIFISHSNNNYYSPIQRSNFKSPISYLNNNCKVFYFSVPFKKVKSNNNNNNNNNNNNNNNNNNNNNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKE3</accession>
    <name>A0A8J4PKE3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000906">
        <property type="protein sequence ID" value="KAF2068618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKE3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="176"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="677" mass="80346" checksum="D78E080A26CACB7A" modified="2022-05-25" version="1">MIENRVLNWLKVLTLGENNKYIFYILEKLSSKHMPITQDSLKTFVNSNKWLSESEYISLTKEVSCLENFSDDDEDIDSFDRKFRRILYCISENNKTLIKESTHSTPPIKKLKSIDQSPTHHTTQSSTPQQTVPSLSQSSSKFKYNRRPKPSETTALDNFSNEYNQREMGDGENLPKTTTTTTTATTTATTKKRSRKEEEEEEKEEEEEEEEEEEEEEEEEVEMQEEAEEIEEIEGNHDFYNEDDGYDYDEDDYDDVDYDETPPAKNQKKAKSKRTKERTFNQHEPIRKKLIELKNMYSRYYSDLPYSNISDSNWISDFNLLKDYSEDIIKPAHQSQLKDKKYIVIHQNWKVFITKFMEKIKIHHPNDYKTEMKSGNYLIYPLTNCRVANMWKHFHYFKRFKLTQKDIIEILNKMLDTGNSDLLIQRCLDYLKRTKNPFFVSKIKFVNYIKTCFLIKEEETRDGRIVEYVSINGDALSPVQWKESYYGKRGNKGSTLLALQQYFLALLEHYMEIETTKLNVFMKKEERKEIFLDIFRSITGSKGREKSAQTEEETTEDIMKALDEDQETQAKARKSKADFLEKTYLDNVGVEKLLHKSQLENLMLVNVKLFNSTMNELQWHRIKIFKELIETTESEHLKQKAKESLEHELLRKYPIAYPSRDTEYSFPIRNTDDHLQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKL4</accession>
    <name>A0A8J4PKL4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">D-serine dehydratase</fullName>
            <ecNumber evidence="9">4.3.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">D-serine deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_010466</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>D-serine = pyruvate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13977"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:35247"/>
            <dbReference type="EC" id="4.3.1.18"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:13978"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DSD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.18" evidence="9"/>
    <dbReference type="EMBL" id="AJWJ01001132">
        <property type="protein sequence ID" value="KAF2068209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKL4"/>
    <dbReference type="OrthoDB" id="20198at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008721">
        <property type="term" value="F:D-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036088">
        <property type="term" value="P:D-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.10:FF:000016">
        <property type="entry name" value="D-serine dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.20">
        <property type="entry name" value="D-serine dehydratase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051466">
        <property type="entry name" value="D-amino_acid_metab_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026956">
        <property type="entry name" value="D-ser_dehydrat-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042208">
        <property type="entry name" value="D-ser_dehydrat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28004:SF2">
        <property type="entry name" value="D-SERINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28004">
        <property type="entry name" value="ZGC:162816-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14031">
        <property type="entry name" value="D-ser_dehydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01119">
        <property type="entry name" value="D-ser_dehydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="D-serine dehydratase-like" evidence="12">
        <location>
            <begin position="268"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00075219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2068209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="392" mass="43802" checksum="ADF2E3B50B1C3FFA" modified="2022-05-25" version="1">MVHHDKIKDLNTPCILVDEKVVKNNCKSMIDRANQLGVDIRPHMKTHKTIEIGKYQVEGLKNQRVIVSTLSEAKFFTKAFKDILYAIPISPNKISQAYEIHCEIDRLHLMIDHIDHITSLVNHIKQTNINGKIWSVFLKIDCGYHRAGANPESEETIRLVDLITHGEYSKYFEFQGIYSHSGHSYKCTTPQEIKKLAIQEATVTGSFGKKLLACGYPCPNVSIGSTPVCCHLPDNLKDLGVTEIHPGNYVFYDLMQMELGNCSVDDVGVSVLTTVISVYPDRNEFLVDAGSLALSSDPGCTHLAPNRPTPNFGIIVGHPNLRVLAVTQEIGKIVSTCDNSPIPFDHFKIGTKIRLIPNHSCLTAAMFSHFYVLDSHDDVNGELKSFVPNKHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKP3</accession>
    <name>A0A8J4PKP3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7743 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010406</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001091">
        <property type="protein sequence ID" value="KAF2068269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKP3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035293044" description="DUF7743 domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="2">
        <location>
            <begin position="434"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="631" mass="71472" checksum="09B6A507D43B380C" modified="2022-05-25" version="1" fragment="single">MYTRASFVLLGVIVFSLLVSCQAQNPILMTDFTDASALNKYSTPSLTTPTCSFSYSLLFSQQFNDGAIIIPLEFPISSTVLISSSANEYLYQVNTNLPTSATTFQVKALSNTQTNTFNINFKCETPPTTLDIQFFNNNTLYDSYSSTTYGPVLTFQVINFFKYGSPLDATFPSQTLVKREVTWFSENIYYIAFDYSVGANKWSDLYDDVIINIQYSENPSWAPASNHIIKSNYKSANNYTLEYSFFGLDSNSNTINKNIFPIVKFTTTENKPNLWYFKTNSPNLLNSRGLICMGNYTKGVIIQTIAKEGLLTDTLTFAYNNITHNMVEKSINYNYLGPTPTFTLIKTDTANRILSSSVTTDTQVPALFVMIKSGLNSLYVYNSDGIYPYGILLRTSNIYTRIDEFFVSILSNGPREFNYDGLVTSFDFGATGLYDTTPPNIQSIQQYRFGNINIFSVHITDDLSGFYRLQGIGDFTSLVSGNLQDGVYEFKLDLEKQFLLNNDLPLCDSAFSCKGLNLKYFKATDNSTFKLDEFKLVSEINRIYWKYNDIDVSEKGLNNVLYICSAIKTYQPHILPATGLRSYTEINSILGVYNNSNGCYQFIVPIHKNYITGNYNAYLWLGYAWEQFSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL53</accession>
    <name>A0A8J4PL53_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_009750</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000795">
        <property type="protein sequence ID" value="KAF2068928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL53"/>
    <dbReference type="OrthoDB" id="5584805at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050715">
        <property type="entry name" value="LRR-SigEffector_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45752:SF187">
        <property type="entry name" value="LEUCINE-RICH REPEAT AND IQ DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45752">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="4">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="413"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="413"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="438"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="464"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="613" mass="67604" checksum="F7DF7F6961D30264" modified="2022-05-25" version="1">MNILQVNVLEGRDLASADTNGYSDPYCTVSLGPQKKKTQICKKTLNPKWNETFLMRLSPSDIKLHIKLLDWDQLSSDDFLGEVFIDIDSLNDSAVWYTLLPRPDHPDDFVKGEVCIKARVVKTSNIVKDKIPSNMITEVNNRVSTYEENGDLLDISGIGFEQFPDFLFDHVPALENLDLGFNGFKMFPDLRSFSKLKSLLLNGNSILTVPGEVLNLPLLTHLSVNGNHLIALPTEISKLVSLEKLEIANNKISELCPEVCCLPKLEELIISGNPLIKLPPNFSNLHSLEILDASGCQLTRLPEDFSLMIKLLEVNLGNNKLIELPNAIGRLTRLVVLNLMDNKLADLPLSIGSCHGLGKLGAGINIEGNPIQSEEMMKKYKIGNDQLMDYLEKRMALSGYSLPDIQKLKLNTSNGGIKTNNNTAPPVPLSALAPIPGPSQQKTKNQYTLVAPDGANQSPPQPTQPTQQQPTQQNSNQDVVTKTIALKNWAISTIRGEIRPKLGKIKTQILRCVQTQEGIGIANTLKQLKPEVEKLKALIPSTYPSPCIPKNPTAGKTFSGNDKLEQLKDLVAASLDDYDVLFETLYQLIPNSQDPPLIVAVAQSLKVIKEIIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLS9</accession>
    <name>A0A8J4PLS9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009296</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000674">
        <property type="protein sequence ID" value="KAF2069382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLS9"/>
    <dbReference type="OrthoDB" id="3797628at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044614">
        <property type="term" value="C:nuclear pore cytoplasmic filaments"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034398">
        <property type="term" value="P:telomere tethering at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037665">
        <property type="entry name" value="Nucleoporin_S59-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198:SF6">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP98-NUP96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198">
        <property type="entry name" value="NUCLEOPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="186"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="213" mass="24380" checksum="CCB191B9641B6F9F" modified="2022-05-25" version="1">MALYNRIPYTPTNIGGETFVSISAMDSYKNKSHQEIRYHDILDTFGSILYLNPTQQQSFDCNNNDNNNSINEKDDIDSTSIPYSDSTIDGQCFVSISAMNAFSHKSHQEIRYNDLLKGFYKGSVKQQQQQLQQQLQQQQQQQLHTKTQQELQKQLEYLQLSQEKLDSALLLHQQEQELEQNKQSITAPTTTTTKTITSPFGDPSCKMSPFGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM14</accession>
    <name>A0A8J4PM14_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="16">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">CYY_009319</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 16">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 16">Membrane</location>
            <topology evidence="1 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="16"/>
    <dbReference type="EMBL" id="AJWJ01000678">
        <property type="protein sequence ID" value="KAF2069365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM14"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000032">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000084">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF19">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 15 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 16" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="329"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="889"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="916"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="969"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1001"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1026"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1067"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="19">
        <location>
            <begin position="22"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="18">
        <location>
            <begin position="119"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="20">
        <location>
            <begin position="854"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="430"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1109"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1147"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1183"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1234"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="445"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1109"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1148"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1192"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1258"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="13">
        <location>
            <position position="402"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="403"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="515"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="558"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="582"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="618"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="699"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="700"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="701"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="802"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="808"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="827"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="830"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="831"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="831"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAF2069365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1278" mass="144699" checksum="699EB3B2C8FE9E57" modified="2022-05-25" version="1">MMDKLFKSSNTKHHNNTIRTIYAGDVDRNRAEYPNNRISNTKYTALSFLPKNMIEQFGRAMNIYFLFIGVLQLFPALTPVNPLSTWGGLLFIFSISAIKEAFDDIGRRRRDKLANERVYTVVRDGKKQSILSESILVGDIVYLENNSEIPCDLLLLSTSETENNSCFIQTANLDGETDLKTRYSIRETSKLSEQRLMSFNGVLECPSPNPDIYRFDSRLSMKVNSKVNTFSHSDWLTVDASNLVLQATHLRNTDYIYGLAVYTGNQTKLGMNKMSPPTKWTKLDRAVNQITVFVFVFQLIMVLIFGLIGDLFTYQLDKKAWYLPPNAGMFKSIVVVPLRFLLLNSMMIPISLKVTMDVTKYTYALFINWDLKMYHRASDSPATANSTALSEDLGQLEYIFTDKTGTLTENIMLFSKCSINGVVYGANSNSGNSNNDDGDDDGNIQLLSPSSSQTALEDPNLLGKLASKSIYEIEFFKALALCHTVVIGQPHHNEQQQEQGEDQVMNNYKASSPDEEALVKASFKLGIKFINRTPSQLTINVMNDSVDSTYQLLHTFDFTSDRKRMSVVLLDERNGVIKIITKGADEVVFQKLDQSNSNNHLLDTYRSQIDEFAHYGLRTLCVAQKEINEQDYRYWFEKYYQKANTAIEGRSSLLSEAYDMLERNLELLGITAIEDKLQESVPQTIHCMREANIKVWMLTGDKYSTAIQIAHSCNLIDDNGKLYTVGKALHDQSNAGAHPAISQKDVEMSIEALYKEIRSLPKNTQEISSIIIEGHVLALALIYAADNLLAVSQLVGSLICCRVTPNQKAQVVKMIKDTGRITAAIGDGGNDVSMIQEANIGIGISGREGLQASRAADYSIARFKYLQELVLVHGRYSYVRSSFVANYSFYKSLFMCFIQILYQLFCGYAGTSFFNTFSLTSYNILFTGLPVIGFVLDKDLPEAIIRRNPILYTYTQEGRLFNRRVFAGWFIRALFQAIFVFCITVGAFAYGSGSTIDYNSVSMISFTGIIFIQSLTLYFESHTITWINHILLWGTIPLYFFCVFFLNSVPTMDTYSVMTHLFDSSSFYFSLVLMVFACIIPVLIIQYIFVLYKPTISEIIHQVRLTSFSGSDSNSNNSNNNGQKRYSSISDEETSAFSNMIDDKDLLNSSGSNNHSNRNSIENPLLGNINERSRIIQYKYKKHQNGNKDGSDNSNSNKNQKNQSDSSSFQKNTKFNTRKVYYEKMVHLVVGPREEEEYENKKLYDQEYNDIDNDNNNKSNSRQAPNSKSNENLPLLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM45</accession>
    <name>A0A8J4PM45_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ADF-H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_009187</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the actin-binding proteins ADF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000651">
        <property type="protein sequence ID" value="KAF2069498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM45"/>
    <dbReference type="OrthoDB" id="25672at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11286">
        <property type="entry name" value="ADF_cofilin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017904">
        <property type="entry name" value="ADF/Cofilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11913">
        <property type="entry name" value="COFILIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="5">
        <location>
            <begin position="4"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2069498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="140" mass="16075" checksum="68D8D9D62C855D1F" modified="2022-05-25" version="1">MSSGVQISSECVPVFNDSLKLGKSIRAIFYKLDQKSEKITVEKTIPRQETNFETIVGELPLQEPRYVTIDYEYKSEDNVTKSKIIFLSWCPNGTPIRARMLHTSSLQSIRRALVGINFEIQASDFSECSHEVFLERINRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMF3</accession>
    <name>A0A8J4PMF3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phosphatidylethanolamine N-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_009708</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000784">
        <property type="protein sequence ID" value="KAF2068976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMF3"/>
    <dbReference type="OrthoDB" id="4583at2759"/>
    <dbReference type="UniPathway" id="UPA00753"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004608">
        <property type="term" value="F:phosphatidylethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006656">
        <property type="term" value="P:phosphatidylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007318">
        <property type="entry name" value="Phopholipid_MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32138">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32138:SF0">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04191">
        <property type="entry name" value="PEMT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="363"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="397"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="441"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="473"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="537"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2068976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="676" mass="78338" checksum="33A13D4586FD09A7" modified="2022-05-25" version="1">MIENTMTMKHQQQQNSSSGSDSDDATVSRKKDTTARLRKKESPAISNIKNKSAAAFSSDSESEGNKDKSHNRLIGVCSDGTRFPLPHTKNPLTLHPVSEWSFFDYAKNASLASLLLLFLNLPGWVFIAWFLFWRFAYNVGLGLILRNQSHTKFFTRWIKVMSQPSHPFYSKLKQFCSSGMGSDYNFDETPSEYNAWLAFRNIVDIVLANDLVTYVVFSMAYFEFDYSWTLIPCYILGGLLCAFTFWAKTDAYRVVKDFAWYWGDFFFLVDQNLTFDRVFSISPHPMYTIGYTFYYGLSLISQSYTVLYVSLFAHICQLLFLVVVEDPHIQKTYPEIVEDPHINREKVKDYFGKDLIVVKNFQFIRAADILTALLIAYTVGLNLFSNLPTWFYIIQAIFWRCTLSFGLGLVLSCQSSYQWWTKKYEKDLNLDKPRAFENWKSIYNICLIMTHVSFWCCFLKYAQIDLNFFGSIFWRQILGVMLILINVWSSWSTYEVLGQFGWFYGDFFIEEIPSNLYYTGIYRFLNNPDSVTGFAGYYGLSLIAGSFPLFALSIFSQVANFLFIKFVEKPHMKKLYGNKVRVQSGISKGIQEIVSELAETSPPIQKFTHYVNKTKHFTERVEKKVGERINNLLEELREKGIPLSSEQIAILKTIKEKYNKHSNSNTTYSSDSDSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMR8</accession>
    <name>A0A8J4PMR8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008622</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000550">
        <property type="protein sequence ID" value="KAF2070053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMR8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="112" mass="12761" checksum="67B61176EF8D7122" modified="2022-05-25" version="1">MESLPVEPHQEQAKISLKIDDDIIVFKNTADLFAQETKVNVEQLNQQHLHFINQYRITKSSKACETEENELNQCLLSKKISSQSSFSICQDSIAKLMKCEQSFLAATLNQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN89</accession>
    <name>A0A8J4PN89_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tetratricopeptide-like helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007656</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000420">
        <property type="protein sequence ID" value="KAF2071035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN89"/>
    <dbReference type="OrthoDB" id="312680at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046813">
        <property type="term" value="P:receptor-mediated virion attachment to host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="580" mass="67666" checksum="AAFBFDFCFAA3F5B9" modified="2022-05-25" version="1">MNSIQRLTRFKYRSRLPISNHVTTNNFTLKSNHSNNKNIKTKIFSTINNNNYGCKYYFTSSKSLFSEQQQKPQQQHEIDVKYQSLIENAVEHFKIKEINFAIQLLDQAIQLCGNKPDAYLLRADIKSEFLRTFPSALHQDAIINDYETAVNLVPQNKKAYVYRAIAKYFGKLPETPLEQMNLYLDKAIELDPDNIDYVVDKVKRAGFLCRIEFLIEPSKRLVKERSKYGALALGLIAFAQGNIPQAIQLIQSVIDSEVNLPKHDQFKDVPLYHGRLIVGRDTQGYTRIRDRYYEANNILFDAYIYHAIVSCLHDPNLSLEYHKKALEIVPYAYHFHCMMGQMTFNSGRFEEAIKHCNNFFQHDPDFHSPYAHQTLTIRGCSYYQIGAIDKAYEDLQISLERNSTEKYTIDNILDRANPILTMIYCLRLFVEGLRGLDKSKFNIEAVIKSIDFDGVLLTEPKNIQQLFTRTRIWYNVYKIIIDESVQSGLVKSSGEEAASLYYRERLFYHLTDYLIDSMYLNYLENCRDIAFDDKDFSKLLDFISQLPYKESKQLPERQEGHKILLNLYKAVIAHIQGKDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNA1</accession>
    <name>A0A8J4PNA1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sulfide:quinone oxidoreductase, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_007687</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SQRD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000424">
        <property type="protein sequence ID" value="KAF2070992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNA1"/>
    <dbReference type="OrthoDB" id="5376590at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070224">
        <property type="term" value="F:sulfide:quinone oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070221">
        <property type="term" value="P:sulfide oxidation, using sulfide:quinone oxidoreductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000034">
        <property type="entry name" value="sulfide:quinone oxidoreductase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015904">
        <property type="entry name" value="Sulphide_quinone_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10632">
        <property type="entry name" value="SULFIDE:QUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10632:SF2">
        <property type="entry name" value="SULFIDE:QUINONE OXIDOREDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0874">Quinone</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035191716" description="Sulfide:quinone oxidoreductase, mitochondrial" evidence="11">
        <location>
            <begin position="25"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="12">
        <location>
            <begin position="42"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2070992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="447" mass="49152" checksum="95E543D0BB94DD33" modified="2022-05-25" version="1">MASLLKGFSLLALTTMSVSTLANGGKSDVALKDIRKENGKTKIVIVGGGAGGLSVASQLKKNFDTKGDIIIVEPSDKHYYQPLWTLVGGGIFDRSKSEKDEKDCIPAGTNWIKDSVVEFKPDDNIIITKNGDKLDYDYLVVAAGLEIYWDKVKGLKENLGKNGVTSNYSFDSVEKTFEYIKGLKDGVAIFTFPNTGIKCGGAPQKIMWLADDYFRSHGVRDKIKVEFNTAGVSMFAVKKYSDALDQLAKNRNIVQNFKHNLVEIKGDTKEAVFETADGLKTEHFDMIHVTPPMGAPSFIKASPLADPATGFVNVDKGTLQHVKYENVFSLGDCSSLPTSKTAAAITSQAPVLVKNLISHKIGKPLEAVYDGYTSCPITTGYNSLILAEFKYGNVVAESFPFDQSKERYLPMLMKKYVFPFAYWNLMLSGKWFGPKGVFNPHPPQNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNJ5</accession>
    <name>A0A8J4PNJ5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GRAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007548</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000409">
        <property type="protein sequence ID" value="KAF2071142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNJ5"/>
    <dbReference type="OrthoDB" id="2162691at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140268">
        <property type="term" value="C:endoplasmic reticulum-plasma membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120015">
        <property type="term" value="F:sterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051482">
        <property type="entry name" value="Cholesterol_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319:SF4">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GRAM" evidence="2">
        <location>
            <begin position="23"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="GRAM" evidence="2">
        <location>
            <begin position="147"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="313"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="383"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="461" mass="51658" checksum="9457BFC6EC4E038E" modified="2022-05-25" version="1">MQPSSSNTSLADMEEESMRSDNEKIQKKLQLEPTDSVLYSLKCRKKQRHGILAITEQHLHFYSSLLGTTKKKKIAFDAIAKIELVEKDGGVKIIKNSGESITFHFKQNETAHLLIEGQWEASGNNPNMRLTESSMGSKQLYTTPTSSALHKHFELPETEVLKLDVPCSFKTTFKHYGKLYLTQNYICFYSHFITSHTRRVIKIRNIITISKPEKKNRHSILVETKTESFLFSSFQDREKVYDFMVNLYNDSKNDTSSLPPTPNLRSRRNSLSGSLSGVPSKSHSRSGSVDQQSPMVENTITFASVIPISDLNNSSNNNNNNNGNNGFRKVSNASSTLSPNNNNSNNNNNNNSSQPSTPKTHSRNNSVNKDKQSNIPPSPPTPLSSSVKTVSSPSTNNNVPSSTTVVVPASEKIANRIEKVDNVIPKQDTLNLKQKELIKEKDDKDKTNQKAKRRVCCFSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNZ4</accession>
    <name>A0A8J4PNZ4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_007201</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000370">
        <property type="protein sequence ID" value="KAF2071485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNZ4"/>
    <dbReference type="OrthoDB" id="15894at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003333">
        <property type="term" value="P:amino acid transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018227">
        <property type="entry name" value="Amino_acid_transport_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16189:SF3">
        <property type="entry name" value="AMINO ACID TRANSPORTER TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16189">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 104-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03222">
        <property type="entry name" value="Trp_Tyr_perm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="226"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="300"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="347"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="381"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="410"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="488"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2071485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="513" mass="57609" checksum="3C11687BA77B8805" modified="2022-05-25" version="1">MEESQGFTSMQDFTVYNEYIPLNEEPNDEDDVTITPQHKEELKAAFGHKTIGFFGGLSLLVGNMTGPGMVGICLQFQQGGWFFTIAGFILILVCSSLAALFMAEAMATLPGNPRFQLRVEFSMLCRFYFGKWGYAVAQVFINVALQATNIASIIVCAQIMDDMLISIFKKTCGLVAYPKVEWMCVNTPVDSSSPFPDSYYMFFTLGYLVVLVLIIPLGFLNLDDNIIVQIGAVILMFCITSSWVIMFFINGLNPSYMPTIGTPRGIAQIMGNVMFNYSYITTVPSWINESKPTVNIKKSIWLSSSFSTFIFIAIGVFGALSFPNMKDDSNILSIINASHMANTFTRILVYIFPFVVLASSIPVYSIIVRYNLMQNNLLPKLVANGIAVILPWTIALPFMTGDGLNQISEWASLFFSSIANFIIPLLLFIRSVYFRKSKKTMSADQRRILKTLVLETVDWEKNQEMLRFEEHHTMFKVFGKLNRDKSNIVAIICLGILGILIPFVIITNFVFPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPG9</accession>
    <name>A0A8J4PPG9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL46</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_008299</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL46 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000499">
        <property type="protein sequence ID" value="KAF2070386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPG9"/>
    <dbReference type="OrthoDB" id="414075at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04661">
        <property type="entry name" value="NUDIX_MRP_L46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000018">
        <property type="entry name" value="39S ribosomal protein L46, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040008">
        <property type="entry name" value="Ribosomal_mL46"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033650">
        <property type="entry name" value="Ribosomal_mL46_NUDIX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13124">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L46, MITOCHONDRIAL PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13124:SF12">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="171"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="171"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="194"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2070386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="351" mass="40960" checksum="25E3BFAD6DC6476F" modified="2022-05-25" version="1">MISSIVKHTSNKNSVLYNSLINSTQYQIRTYAKQAPAAAAAATTPVVDEPRGPKEIIEDTRSNGVKLFEKFKIPRIFDHGDPKLKEFQHLMKCANERIQHKDAEKNQDNTNTAGAYQGKFRLTSSVIIERFPSLVPLPNEYEQKYLDSCERLEELIRREPIHMMSLVEDLPEMKEDDTKDQSNTQADELEEDFTNYTPEPRTTQDDIQQNTQSLNRHLDKSLYLIIQKAGSRYTWQFPSTNWVNGESIKNTAERALRDSCGSSWKYWIPSQSPCGVFKYRVESELQDLLKAEGIKEFFFRSHYFGGDLKFNSKIVLDYKWVSKEELKDYLDEEYYESVVQFIYDDCYYGYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQZ7</accession>
    <name>A0A8J4PQZ7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DDRGK domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_007437</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DDRGK1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000396">
        <property type="protein sequence ID" value="KAF2071255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQZ7"/>
    <dbReference type="OrthoDB" id="2285710at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044389">
        <property type="term" value="F:ubiquitin-like protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000143">
        <property type="entry name" value="DDRGK domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019153">
        <property type="entry name" value="DDRGK_dom-contain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050899">
        <property type="entry name" value="DDRGK_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48176">
        <property type="entry name" value="DDRGK DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48176:SF1">
        <property type="entry name" value="DDRGK DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09756">
        <property type="entry name" value="DDRGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01128">
        <property type="entry name" value="DDRGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="34"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="87"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="120"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2071255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="332" mass="38143" checksum="21103DC69D5E34F6" modified="2022-05-25" version="1">MDQIFIIVLGVCLLVLGGLFYLVIGPKKANNDNNQQVDNDVQLENNNNNNNRAAGGRGLARMNVRNRRAPTPAQNNNDNDDTSSGSDLDDSDGDNDFNDDDAQGIALPTHTASGKKIGTKKLEKLKLKEEKRRHREYEQHLRDEKKKRQEEQEELDKERREEEKEKERLRKEEEERIRLEKEKKEDEEYALLRGDIQLEDSGEQKQTEDDERSLLQEFIKYLKENKICLLEDIAMEFNLKTMDVIERIKTLDKEGLISGVIDDRGKFIYVTKEEMEAVARFVNKKGRVNIEQIAAESNRLIDLNVKVVSKPCTSEETTGDSTTTISSQITSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRB4</accession>
    <name>A0A8J4PRB4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Gamma-soluble NSF attachment protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">N-ethylmaleimide-sensitive factor attachment protein gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_007835</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNAP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000440">
        <property type="protein sequence ID" value="KAF2070849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRB4"/>
    <dbReference type="OrthoDB" id="9984275at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005483">
        <property type="term" value="F:soluble NSF attachment protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000744">
        <property type="entry name" value="NSF_attach"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768:SF2">
        <property type="entry name" value="GAMMA-SOLUBLE NSF ATTACHMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768">
        <property type="entry name" value="SOLUBLE NSF ATTACHMENT PROTEIN SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14938">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="280"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="282"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2070849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="323" mass="36564" checksum="D266C3EC1EAD176F" modified="2022-05-25" version="1">MMNTKRIEADAAMKDADKLSTKTLFRWKPDWDSASFLYEKAANGYRTAKVYDLAKYCFIRLSMCQTHMDVFYLAAKSIETAATMAKELKDTQECVRLLLEASKLYRTNGNSFQSADTMTKAAKLLEETDLDQCVQLLLDACELFELDDKDHFSGDTFKQTISMLLRHKKFMEAIDLMLLQNKVFLKLDQKHDLNKSCLSIVVISLLLDDVVGAKNKFTQFLDYTGFIHSDEGITAQELITAYDKNEADTVKKITQKHIFNFLDNQVAKIAKSLQLKAPSAVNPMSSNHNNNSFSNPTPTTNTPSNDTPVGDSHHHDEDEESVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRE1</accession>
    <name>A0A8J4PRE1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Calnexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">CYY_007127</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Endomembrane system</location>
            <topology evidence="16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the calreticulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000363">
        <property type="protein sequence ID" value="KAF2071555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRE1"/>
    <dbReference type="OrthoDB" id="1938156at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.250.10:FF:000001">
        <property type="entry name" value="Calnexin homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000048">
        <property type="entry name" value="Calnexin homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.250.10">
        <property type="entry name" value="Calreticulin/calnexin, P domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001580">
        <property type="entry name" value="Calret/calnex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018124">
        <property type="entry name" value="Calret/calnex_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009033">
        <property type="entry name" value="Calreticulin/calnexin_P_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073:SF1">
        <property type="entry name" value="CALNEXIN 14D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073">
        <property type="entry name" value="CALRETICULIN AND CALNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00262">
        <property type="entry name" value="Calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00626">
        <property type="entry name" value="CALRETICULIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63887">
        <property type="entry name" value="P-domain of calnexin/calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00804">
        <property type="entry name" value="CALRETICULIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="14 18" id="KW-0143">Chaperone</keyword>
    <keyword evidence="12 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 18" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0430">Lectin</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 18" id="KW-0732">Signal</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035338521" description="Calnexin" evidence="18">
        <location>
            <begin position="23"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="447"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="215"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="468"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="220"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="237"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="257"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="282"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="481"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="492"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="507"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="110"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR601580-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAF2071555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="22">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="516" mass="58473" checksum="BC8E3671104963B7" modified="2022-05-25" version="1">MNKNSKLQLLLILSIIIGTCLSFTPSKDTVFLEDFQGTSSRWVNSQQAAYNGIVEIRASVDPIDASDKGLVLTQKAKKYAITSKLIKPVDNKGRDLVIQYEVKLQTGLDCGGAYIKLYSYSDNFESENVDEKTPYSIMFGPDKCGQENRIHFIVRAKNPLTGEHQEKLITARPPIRIDKIAHLYTLHIKPDNTFSIFVDGVSVLDGDFHKDFTPAFNPPKEIDDPSDKKPSTWVDEETIKDPEATKPEDWDETQPARIEDPEAVKPDDWQDNEPELVPSDETQPEEWNEEDDGEWEAPLIQNPKCSLGNCGEWKAPLIENPLYKGIWSHPMIPNPEYIGEWKPKQIPNPDYFEVENPYIVEPIGAVGIEVWTMSQDILFDNFIITHDKSEADKFASETFYPKHKLEEERQAAKEAAEAAEMDPSESFVDMALSYFNIIQEQATTNPLILVVSVSALILPIVFCMTRSSPKKPVSTPAATKVENKVEKKQESSSEEEEEEEEEEQKTSVKKRTNKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRK5</accession>
    <name>A0A8J4PRK5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006990</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000349">
        <property type="protein sequence ID" value="KAF2071690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRK5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="556" mass="65188" checksum="B1954A1F7E412D37" modified="2022-05-25" version="1">MVDDLLIGSYKLYDQCYLYNNVNYLDQSVNSNPLDKVEILSCPSFRNKEQVLQSITYDSNKNSFSKFKSFNIDIIYNQYNVINQDYYSISNFKQLSIYKGDKLVYSGNLLPEPYHSLENQEYNRINMKWLKTTTSNNIDVQYVLTVSFKNTLFLLFFNHLLNNLLHVHSFKTHYNIKHFKWSPLYNNLLLSTEDNQLHLLSFNSFIYLDKDYKIPKYNESIYKKDDNNNNNNDDGDEQQEYHLIEWIDNEHFIILCQHIIRSNEKVDIQLKEKKDLFSLSTQVMPKISFLNSQQQESEIIQINSGNSNSNSSLISSLFMIDKSDNNDTLFNISNIKENQHQHQHQQQQRNNFTIYQFKISKQQIQQFNIFKSKDIQLQNPNLSLYSSDNRNLIISSTLSHFIYFLTIDSTSNIIQYQYKLDISSGSGSSTLDSNNGNNQIYCKGISLIQNQKHFKLLVMQGEKCSSITSTLLPKPYSLFTVHLNVYNLINNNNNKNNNNDNNDNQPNLSIFFKEFQLFQNILLDKIGSIERVLDTHTKKLDDLDNAIKNLSNNNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRS2</accession>
    <name>A0A8J4PRS2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006515</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000304">
        <property type="protein sequence ID" value="KAF2072172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRS2"/>
    <dbReference type="OrthoDB" id="24371at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052050">
        <property type="entry name" value="SecEffector_AnkRepeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586:SF1">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="574"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="588"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="623"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="937" mass="109926" checksum="5A507E830D249F95" modified="2022-05-25" version="1">MDTNNSNREILFFQIFKNVAIKHYIFKFLKTTISGDIQSLDYIREEQRPVNYKTKHYFDRSMNQSWLVENKHFNLLIDKMTHPFFKDSFHLSLESIGKIVQTNTDYNIFRKIYDLVPHLFKSANIEAFQSYSKGKAQYSRIKRKKPLLDLACRAGNVNIIEFLISKGYKSTDSALKRLVKRNHLDAFKLLLSIQRRPLPVVQQTLLLNLSILKKHFPIVDYLIERFGIGISTSQTLLNAISVGNKELIKKLVSANEGELLNNYCMYKLAEFGDLEIVQSLLESRPFLYGPLLLDHSFSSGNFELVHFLESKTREHRLYVTCRAIDFSASKGDMKMLQHLYNNYPVVSCSVQGFIFSLKNNQTDAAIFLFEHYGNALKNVDNPFKFILMHQNVTLLKYFLEKAPQFKKEELSSSVVFKLIKNGNLELIKIVVEQFPLPDYMNWSSILFQSNIELIEYFLLQRKVHIDIYDLKKCLDKEMFQSAKFLIENQQVFSNPNPLVNANQFEEDDYELDEEEQADMVNSQEDYQENDFQDDELETDQVSKDLKAKAMDMLDYALYVKDDIDIDKDDYLDNDDGENYFDGNNGVWQDDDQDDADDQDDDDDDDDDLDDGENYFDGNNGVWQDDDQDDADDQDDDDYGNIGVFQDDSDKYREMEINFNPPGGYIESKSKTKLKIFGKPKTHENDNFEYTVPRYSTIIVDSMEKLVFLFSNFKYQDKSFSLSSVDDDDDDDDDQEFNPIIFGRNSNEMVLILKYCIENSIWFQSTPFILDFIENNNIEMLKLIRDYAKTQIIKKELKGKTVLQHSNIGYSFMDQFLLERYIEKQNGQLFKSNPEFFKNTLLHHPKTIFYLYFSKVYGLISRDDFKCITFADVKHINVIRFLLYNFKDISFKCDTPLLEIFTKYTGKKQLNFLIYTLKEPIPTHLKGKIVNLLQWYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRT4</accession>
    <name>A0A8J4PRT4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005933</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000251">
        <property type="protein sequence ID" value="KAF2072745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRT4"/>
    <dbReference type="OrthoDB" id="15836at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="14699" checksum="417889EA287CE937" modified="2022-05-25" version="1">MGCFEGNEIKVQIIYLICILLCILGIINAFSILIIGIIICIGTIGVAICGAAGARFKMDKLLFYFTLGLLLLMALCVVSIAWQAVKAKGFSWWMLSNFVLMIVYGTGIFVTYLLRGRTLFRSAGTAQNASFQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS22</accession>
    <name>A0A8J4PS22_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006174</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000273">
        <property type="protein sequence ID" value="KAF2072507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS22"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3110">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="179"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="295"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="373"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="21"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="43"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="185"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="204"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="295"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="484" mass="55174" checksum="81E0D02081A21641" modified="2022-05-25" version="1">MDTNFSLDEQSLYNRDRENDTFSSPINSPTNFSSNRAEERNDSSPPRSYHSSPIRQSFTSGSTYNASSSTSNASGTALDPKYKLTIIPKFLKKLDRLYTVDTQFDNDDPRVKEHYLLVGNSVKTDKIATLIKESYIWKNLNNLFNVVISYYHSKGILFRLRRFEGKKYYPLIRRSESSNVKGYKRGLEDQERDEPSKFNKIRYSSDNINSNNNNNDNTSNSNHNYSSGNNNNDSNNSNNNNNGNTSISNSSGSINNSYSGSSINNSYRSSNLNNNTDNKGNISINSSSGSINNSYSNSNYNSSNNSYSSSNYNNNNNYSNNNSNSNTLNNNSNNNNSYSNSSNNTNYGGNENKGLMEIMKENLEHVGRTLIEKGKIKDQFETMRKENKELKEKIEKIKAENDAMKIDIRDLKYGYTKLKDYHGNLKNDYVSLRDQMEHFKSEIQHLKANQITDQAGIKNLISRNEFLKSENQFLKSILPKPPYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS93</accession>
    <name>A0A8J4PS93_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS17 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_006640</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000315">
        <property type="protein sequence ID" value="KAF2072050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS93"/>
    <dbReference type="OrthoDB" id="10254436at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00364">
        <property type="entry name" value="Ribosomal_uS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.1000:FF:000001">
        <property type="entry name" value="40S ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_uS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028333">
        <property type="entry name" value="Ribosomal_uS17_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032440">
        <property type="entry name" value="Ribosomal_uS17_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006345">
        <property type="entry name" value="PRK08572.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03630">
        <property type="entry name" value="uS17_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744:SF9">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16205">
        <property type="entry name" value="Ribosomal_S17_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00973">
        <property type="entry name" value="RIBOSOMALS17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS17 N-terminal" evidence="4">
        <location>
            <begin position="5"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2072050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="155" mass="17731" checksum="8D38E20A07C8D6FF" modified="2022-05-25" version="1">MADIQAEKAFQKQQCTFKSRFQAKSAKGKEGIRYYKSIGLGYKTPKEAIEGTYIDKKCPFTSDVSIRGTILKGTVVSTKMKRTIIIRRNYLHYIKKYNRFEKRHSNIAAHASPCFNVKDGDSVTIGQCRPLAKTVRFNVIKHEANTAAKKQFKQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT70</accession>
    <name>A0A8J4PT70_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Long-chain-fatty-acid--CoA ligase</fullName>
            <ecNumber evidence="6 7">6.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_006599</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.3" evidence="6 7"/>
    <dbReference type="EMBL" id="AJWJ01000312">
        <property type="protein sequence ID" value="KAF2072079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT70"/>
    <dbReference type="OrthoDB" id="1700726at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd05927">
        <property type="entry name" value="LC-FACS_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045311">
        <property type="entry name" value="LC-FACS_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF33">
        <property type="entry name" value="AMP-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="8">
        <location>
            <begin position="80"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00026121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU369030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2072079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="664" mass="74461" checksum="A90E63694CBA9191" modified="2022-05-25" version="1">MSDHPEVDTHGSPEFLAHQTFEVDAQVQGETRTRRNKKYPDLISVPDKNTNTLYDLYTRHKDSDRNFLGLRELLADGKRGDYKWITYKTACSRAANIGSALVGLGAKRGDRVGIFSINRPEWVISDMACMNHSFVPVALYATLGPNAIEYVVNHSEISIILCEGKNVDKMFTLGSTTGIKTIISYDKQTEATVNKFKDINIDVISLEDFEKTGEASPQPHDPPKPEDLCTLLYTSGSTGNPKGVMLTHINMVSEVAGAHYSPANMTPEDVHMSYLPLAHSFERAVVSLLCYIGGQVGFFSGLIPELFNDIQVLRPTFLVGAPRVWQRLHDKLWLTVNHDSWLKKFLFNWGLNSKQAALKAGGATPIWDKIVFSKTKEKLGGRVKFILSGSAPLDPKLAEFLRACFCCPVVQGYGLTENVGGACVGYPEDNEVGHVGPPLACTEVKLVDVPEMNYFSTDKPFPRGEVCLRGPNLFVGYYKDAEKTAEDLKGDGWFHTGDIGRWNDNGTLSIIDRKKNIFKLSQGEYVAAEYLESIYVRSPLVSQVFVYGDSLNSFLVGVVVPDFEVLEKLVQAKFPALNPKDQQALAKSKEIYTEIMESFEQCAKDAKLFGYEKLKHIYVEHEAFTEDNGLLTPSFKPKRPQLKERYQSTIVELYRWYKEKHPEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTB7</accession>
    <name>A0A8J4PTB7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">riboflavin kinase</fullName>
            <ecNumber evidence="2">2.7.1.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_004448</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.26" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000157">
        <property type="protein sequence ID" value="KAF2074238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTB7"/>
    <dbReference type="OrthoDB" id="276388at2759"/>
    <dbReference type="UniPathway" id="UPA00276">
        <property type="reaction ID" value="UER00406"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008531">
        <property type="term" value="F:riboflavin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009398">
        <property type="term" value="P:FMN biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.30:FF:000005">
        <property type="entry name" value="Haloacid dehalogenase-like hydrolase domain-containing protein 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.30">
        <property type="entry name" value="Riboflavin kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023468">
        <property type="entry name" value="Riboflavin_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015865">
        <property type="entry name" value="Riboflavin_kinase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023465">
        <property type="entry name" value="Riboflavin_kinase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22749:SF6">
        <property type="entry name" value="RIBOFLAVIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22749">
        <property type="entry name" value="RIBOFLAVIN KINASE/FMN ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01687">
        <property type="entry name" value="Flavokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00904">
        <property type="entry name" value="Flavokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82114">
        <property type="entry name" value="Riboflavin kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Riboflavin kinase" evidence="9">
        <location>
            <begin position="24"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="173"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2074238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="201" mass="22107" checksum="73AF9B740F1F46FE" modified="2022-05-25" version="1">MTIERGVLLSSNISNSLDTSGAGFLPLFLKGTIIKGFGRGSKELGIPTANLPVEEYEDILKDIPVGVYYGWANVEGLNNNQVYSMAMSIGWNPFYKNTKKTVEVHLIQKFDQDFYGSQLNAIVLGFIRPMCDFKSLDELIKAINDDIEYAQTCLSTTENTEFKNHTFFNSTLNNSNSNNSSSGSDGTDDNNSSGVSVLSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTE4</accession>
    <name>A0A8J4PTE4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005674</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000233">
        <property type="protein sequence ID" value="KAF2072996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTE4"/>
    <dbReference type="OrthoDB" id="21000at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="329"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="163"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="191"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="332"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="352"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="385" mass="42386" checksum="E247B66A46B7B9AC" modified="2022-05-25" version="1">MSMITVDILTPAFHFPSTFQRSEILSLCRSNIGVDDLEFIPINNDRIGMIIKDSNFSMETVEGIYWQNYYLFTQLSRATESLLYFQDKHTHDQETIASLKKEIAQLKKSENDLIVKLEEQSDKHRLAEASTDKVILDLQQQLSASINIIKSLSQLKEHMNPLNINSSTSNNSPPSSSPVLVGIDAIHNSTGNSNINSNSSSSVSYPYKNSSCSHPNISKSPSNSSGSLMDKKSPSIKPILYNLKFMASPTNKPCQCSQSFPPNISLSNSNSSSNLYSNINNTNNGSTPPSPLLSTMIKPSALKETSKIKQLINHYVTLCTISNNGFKKEDGSNTSTISNNDNTPVVDERSINTNSSNQQQNDQQNNTSSNNNSSSSGSGFIKIYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTP6</accession>
    <name>A0A8J4PTP6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005454</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000218">
        <property type="protein sequence ID" value="KAF2073227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTP6"/>
    <dbReference type="OrthoDB" id="16743at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="395"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="418" mass="48711" checksum="D79F6417DF4D0224" modified="2022-05-25" version="1">MESSSNNNNKNNINENNNINQNNNNNLSNNNTSSLINNGAPLIKGIKIRNYMTKDKDIFSKICRLPHDINLNTLKAYSICYDYSIRRSMAQNGYIYVAEDKDGTNVMGGICVLEKKLRLGGKEKIFFYPFDAIVEPKFRSYRILQRLTDYATNLYMEHSDYDPIIYTSTSVVNTRMDKFYNNTAMVKIIDQVQHAWKVDIKLQPSQFCYDSRFKIWKERDDEIVKKKLNQYFKDYEMCPVEFDDLLLNKFWKYTYFAQWTQGDKVYEASISIWDQNKVCTLIDLEKKENTKLKFFQIFGCYTSGNPENVKIDIFQELLKHVHNDCYHNGIDFLFIGLSKTDPIDKYFPLLSGIKSLNFALHFCVGNTETKEEALALSNNPFFQDPRDYGIVLLHQSSPSDEGNENQSSLIYNPISSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUF7</accession>
    <name>A0A8J4PUF7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">E3 UFM1-protein ligase 1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_003630</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UFL1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000116">
        <property type="protein sequence ID" value="KAF2075063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUF7"/>
    <dbReference type="OrthoDB" id="10258297at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061666">
        <property type="term" value="F:UFM1 ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990592">
        <property type="term" value="P:protein K69-linked ufmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032434">
        <property type="term" value="P:regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018611">
        <property type="entry name" value="Ufl1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056761">
        <property type="entry name" value="Ufl1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056580">
        <property type="entry name" value="Ufl1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056579">
        <property type="entry name" value="Ufl1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31057">
        <property type="entry name" value="E3 UFM1-PROTEIN LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31057:SF0">
        <property type="entry name" value="E3 UFM1-PROTEIN LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09743">
        <property type="entry name" value="E3_UFM1_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23659">
        <property type="entry name" value="UFL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25041">
        <property type="entry name" value="UFL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25870">
        <property type="entry name" value="WHD_UFL1_5th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="E3 UFM1-protein ligase 1-like N-terminal" evidence="6">
        <location>
            <begin position="3"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="E3 UFM1-protein ligase 1-like" evidence="7">
        <location>
            <begin position="537"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="domain" description="E3 UFM1-protein ligase-like C-terminal" evidence="8">
        <location>
            <begin position="659"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="397"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="510"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="402"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="437"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2075063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="765" mass="87454" checksum="25B535A33F61932E" modified="2022-05-25" version="1">MEELEKLRQKLYSIQKEQSAQKLSERNCIEIIVKMLELKMIDIIHTLNGKEYITPKQLELEIKDEILRSGGRVNITDLQPLLNIDISHITDCVNKIIKKDKSIQIYHGEIMTTYYLDSVVEEIQEQLQEVGKLHINDLSLRFDLEVDLLTSAINPRLGRLIHAVFDASETLYTQAHIDRHKHKVMGLFSSVTQPIIIAHLVRQYQSGDYQLNERMAFGQLQELIAAKRINGIIQGKGSSAEYIPSIFSQSRSKWIESFFNQNSYIAYESLTKLQIHDPKAYLKQTFKNGVALSTCFIDQLVVDKVDDSITEIIDNIGWLDVSPLVPPPLSNKDMEMVIKECPNMKCTPEKAIVLNEYFVVSTTFIDRCFTLLQQSIQDKLEKQQKAMENLAVSKDSVKDINEISSPPTKATSNKKGGKSKQGAPGDLDDSDEDIKPTKKGGNKKKGGNKKKNDDSDDDDQPNKKGGKNQQQQQKKVDHLQEIIQLLSKWYESMEEELIQSLSQYLRPKVNQAWENMVKEAREKLENETMKSRKQQQQQLSTQLNTLYANYQLFKNGMQEMDDEKSLPNLTKHLLKTVCTNIANLLVEINANYHMVEFSSIETPAQRSAVLQSLPSQVSKSLDKLFASLSKSSIEEFQDTLDTVCTHSQIKLKQLDKKSERALLDNHEKELKEQLAHEQDFGNQFQIIVNLFYIKLKNHYVYAPPRSIGSLVSVICQDEETDQVLKDQLNTLHQEVVKAIVNKSNSTLEPETTTILENIKTLLLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVD1</accession>
    <name>A0A8J4PVD1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ras guanine nucleotide exchange factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_003071</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000091">
        <property type="protein sequence ID" value="KAF2075648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVD1"/>
    <dbReference type="OrthoDB" id="10255964at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF347">
        <property type="entry name" value="RAS GUANINE NUCLEOTIDE EXCHANGE FACTOR O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 6" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="16"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="11">
        <location>
            <begin position="136"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="12">
        <location>
            <begin position="380"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="9">
        <location>
            <begin position="649"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="549"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="257"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="561"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2075648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="869" mass="99011" checksum="BC702BB335C74642" modified="2022-05-25" version="1">MTTTSKSIALIQSLTCSICNNLYKDPNTLVPCGHSFCFECISKDQASRNIKNCFQCKQEYQSHIVNHGLKQMIDALEQRGSFSMSSSIGGGHSDISSSASSLGGGGSSNSLLNIIEGLGEHRYSTSDLRSSTGDLANIKYCTEHFEEYYSFCEPCQCPICPRCLLTSHNRHGTVVLTKEYLVTKMNEYKSIVEGFKKKISLYQDNIVLYQKEVSLLDGSYNQCKMAIQMMVANLHKALKYRETYLLKEIGTIHYTSHFDLTDRAKTLEGEIEEMEKLISQTEKFRDADINSHHLKFEFLEQFYISRLQSKKNVTTDLKPLYKTDLLFYKANNERITEIINNTLGNISLLTYPLDDMDNVNIWDEPKQNIYIEFVRIGNEDVEIVKYGSLNKLIERLTLVDDQNYSNVFLLTYHSFCTSKKLLKKLIERYTPPDSLEAYNVTTSQITEIQSRVRAILVKWITEYPQEYDQDTINLFQNFNNRMQSEYNSIVEIENLQSTLEEQFAQQNQLVQQQAQQQALNQSSPTSQSPTLTSFNCKLSTSPLNLNSLSLINEYSPPPSPRHSTSSSSSSSSPRLSALKPLNLSGFNNNNNISLTSSNSNNSISQSNNNSFNLGSSGQSYFSPPQSPTSLTLIPSKISNIKNLEFSNIDELELAKQLTLMEFEYYCKIKPVDLLTGVDLKHKSPTITNMLERFNNISTWVSTTIVHAQHLKGRVKIMNKFIKVAEHLKNMNNFNSLTAIILALQRSTVTRKELTKQSAKAFTDLEKLVSSDDGYKQYRTKLAQLSPPCIPYISVNLTDIMDLERKNPPNVSVKVSQFKSCEFINFTRRQLISRAILDLMKYQKFSFNYMFNSIIQEYLNLHIDSDSSNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVF7</accession>
    <name>A0A8J4PVF7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003914</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000130">
        <property type="protein sequence ID" value="KAF2074768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVF7"/>
    <dbReference type="OrthoDB" id="16596at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861:SF1">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="48"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="248" mass="29113" checksum="767DA4BBDB9BBCA5" modified="2022-05-25" version="1">MQQNLYDDDEFFNKYLEYPRNTNDISGIQEWDSYSRFFPNMKGMKICDIGCGTGRFSRYAIQQGASHIIAYDLSRNMINKAKQLTSETDSKYIDYQIEDLETFSLPTKQFDMVFSCLTLHFLLDLDRIIANIYQSLETNGHFLFAVEHPIYTSSIKQPCFIELESNHLIWPVSDYQDEGSRKTNWVKGQVIKQHRKVETYVNTCLKHGFKIDGISEWGPTEKQLKERVDLKNENHRPMFLTISCIKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVH4</accession>
    <name>A0A8J4PVH4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000200">
        <property type="protein sequence ID" value="KAF2073504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVH4"/>
    <dbReference type="OrthoDB" id="10006997at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="489"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1163" mass="132306" checksum="47087A3409E0CF60" modified="2022-05-25" version="1">MNKIEVSSESPGGFDFEATIDLFDNSNSASSPDSSNSPNNNKSIYDGVLKTNLLVPMNQAEKKRKLLLIVLVDKSRSIQTAWFQIQSTLLNLFTIISNTDQEVIAEILFFNNICFRLAYTPDNYRDLILAEKPNGKTCFAAAFDSVSDVIRQYYGKIKVGECDTDTAIVLLTDGSHTTVRDHKNAFHNLRNLAEDTISKHKCQINAHTMGFTANSRFNDLDGIRKLIGTKRGIYQYCESSEGTLALKEKLTFILKHALSNSTSNAMDYFLDITMKTKDSLGEASINVLFNDKDSVGVNNKSQKLLPDANGYCESSVAITSNVYQKDLQVLLQVKLKCGEKELVKLIEVQPSLKTVGKETIYYSGLMRLTNQTNIMFDTIKKVSESKNPSDMLKEDLLKEILTIQRELSNYDNLQIMKFSPKVRSEVVELKKNCQELATQLLDIINSWNRSSWTSTTSARVADITYQYMFKNTGRQRRINLKVARNSNSINKDSANFKQLEVDIDKLEDDLTEEQTKSLESSKQLFSCVLSMCDWVEVLEDKDCMGFGLSVQRPEVVIDDPTQLRIVEVSSTFLAKSSIEDAIALAVEVHGQEKTTGGFEVGQNKTSVAVRGRGREPINSWLPLYLNKEHWKPIKYQLKNIIAYFVTLDPMAFSFDQINALFLVCGTLISKDDIGERQLQLIIQFIRTLREVALHFNFIPKMLQQLNAILNSSIYLVSEQPKNLYVLISYLLVLSNQELESLFKTEQDWFRFWQHVLESALRRGCYAFFANMEQNKIDSYLSQVIFGKVLTEQEYNIQYPDQDTLDQKVITNGDEQQEKQVLNDSQLFDLVLTYKSTLINAPLVDGTIDYGSLLNIKQMHKTPNDKTRENHTVLNKDHIDTINTISNLFESKGYPNSNTLLNCMKFVKAFRSLDKDQDVLTTFNVIDDKYGVPPIEFIDSIKQGLNVASNNSGLGVYLNTVNQLYCSSAPKGATELLNIIRAMIVQGASYRINKHASHAVNSGIFTDTAADPIQCINVILDNVNHRLEEYLTKVRVALMFSAQTSNALKSGSVYEFTHSIPHVGHLHFISFVKSMMSFEKIPFGEEKMVVFLSNVYITQDDQGQLSAIQIKNMQWKYSKRYRAMFVHRFLESTVKYLEQRVLNYQYIYENQQSQKLTLLDDDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW54</accession>
    <name>A0A8J4PW54_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">IPT/TIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_004495</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000159">
        <property type="protein sequence ID" value="KAF2074210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW54"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052014">
        <property type="entry name" value="Dictyostelium_Tiger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31341">
        <property type="entry name" value="IPT/TIG DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035324929" description="IPT/TIG domain-containing protein" evidence="5">
        <location>
            <begin position="23"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="822"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="6">
        <location>
            <begin position="695"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="783"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="783"/>
            <end position="811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2074210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="841" mass="93413" checksum="788A61050ECBA013" modified="2022-05-25" version="1">MIKNILVILVFLCVLQQQYVQAQRFTYRTTITNANPSTVSSQLSDEYLYLFNPMNLYRYSLSSLPFQIEKGVTFVGAASSNSFDYITPVISPRDTLWFNIDSKYGLMYYNQDGMYEFSNMNSSSILTNAGFVIDAIDSISVFNFLQPYHLCTYSTQNKIVRHNDFHIPTTPTNLVCDFKNFKCYLTTINSFLIYQISHTENPILIENVSTQDSFIRRAGIISLDSCPEKLVYYCVESDQDIFIEGRFNNGSVLFSNLVGSKLAVGNCGYTEIDNIEGQIFVITTLNYAMSCDVFGKNCEKMKLSQLVKNVGMNLIKSSDSSVENHLVFPQNDNTFLFFTYSKICPKGCGGNGVCIEKQCLCKDARHTNSFCDTTPLVSDIKPFVTDDGHLIWNITGDFGLPLIQSDSLIKMKSSGNTFSYSLTSATFSQNVIQYKPLLYVGNSVQLTLEINGHNSIENTFTFLYPPVTISGYRQEKEKVYLVGDNFATCYGYLISSGNHQLQSSSFNRTQRELILPKTIEPNQLINYQISPTLTGSISIKLVPFVESISQPKHDHGDTITISGWFFSTSPKVYINDKLISSTFTDKIQVVLPNGLYSNLKISNGLFNTTVALVYPNPTIEKVEHVSESLIVLTGSNFGFNSTAISISLSNKPLTIQSLNSNQGKININLLNDSNKGTLQLQVDNKSVSKSVNLKPVIVSITPAKPSFNGETITITGKYLKDSRIKQTINSLSNILECNEISSNSKTCKLGKGQGIFTITAISPKLIDSDQDLESLQYNLEYKTSPTTTTTTSSTSSPSPSSSPSTSSPSTSGSQVPNSCTKISITLLNYFLFIFVSIYLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWC7</accession>
    <name>A0A8J4PWC7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_003867</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000127">
        <property type="protein sequence ID" value="KAF2074841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWC7"/>
    <dbReference type="OrthoDB" id="198619at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01886">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03713">
        <property type="entry name" value="EFG_mtEFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000001">
        <property type="entry name" value="Elongation factor G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000514">
        <property type="entry name" value="Ribosome-releasing factor 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053905">
        <property type="entry name" value="EF-G-like_DII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035649">
        <property type="entry name" value="EFG_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004540">
        <property type="entry name" value="Transl_elong_EFG/EF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00484">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43261:SF1">
        <property type="entry name" value="RIBOSOME-RELEASING FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43261">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22042">
        <property type="entry name" value="EF-G_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="7">
        <location>
            <begin position="41"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2074841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="746" mass="83310" checksum="7A34E11327C38BF1" modified="2022-05-25" version="1">MLKGLTSTFRLSQAFPSLNSVHHRCFSTFSNINKKVTSELNKYRNIGIIAHVDAGKTTTCERMLYYSGSINRIGEVHKGDTVMDYLKLERERGITIGAATVTIPWNEHRINIVDTPGHVDFTVEVERSVRVMDGSVAIFDAVAGVQAQSITVWNQSERYKVPRIAFVNKMDREGATMEKTLKMMVDRLQTVPIPIQYPVSAGPSFEYVIDLIDQKVLSWNGEKGEVIERQELSEFGSEIRDKAFEKRNELIQKLSDMDEEMLQLYLENDGDIEKINNTQIKQALRRVTMQMKGVPVLFGSSLQNKGVQQVLDGVVDYLPSPIDRDPPVAKDPQGKSVVVDSTTKGNLVALAFKVVHDKKRGLIVYTRVYSGILKSGSNIYNSTRKQKERLNKLMQVSANEMEEITELKAGDIGALLGLKNVCTGDTLVLDNEKAPKLVLKGIQAPPPVFFCTLEAESEGEIPELLNILAIVQKEDPSFQFQVTDDQQILISGMGELHLEIIKDRLDNHFKINTRMGKMQVSYKGTITCPVGEHIQQSFDVQTGSGVKSFFSGISINLEPNQPGAGNEIVFNLDQELIDSMEKFVLDRIKDAITEGLESAFQRGLPLGFPVVDTKVTIDKINYISETESPAAAFRTSTIKAFLKLGEMAHPVILEPMMKIELSVDEKYLGGVLSDLSRQRRATINEVGMEKNVHIISAVVPLKEMIGYATQLRSLSSGNASFNMEFLNYGQVSNNEKEKVLKEIRGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXW0</accession>
    <name>A0A8J4PXW0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">SNF2-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_003824</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000125">
        <property type="protein sequence ID" value="KAF2074869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="19745at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.850">
        <property type="entry name" value="SWI2/SNF2 ATPases, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF22">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="1219"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="1399"/>
            <end position="1558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="118"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="229"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="812"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="937"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1253"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1522"/>
            <end position="1556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="19"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="122"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="183"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="817"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="843"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="869"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1253"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1276"/>
            <end position="1290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1291"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1309"/>
            <end position="1319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2074869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1561" mass="172432" checksum="3446FF3E28C3C546" modified="2022-05-25" version="1">MGLDNQDSYREENVKRLKSSNINTSNSNSNSGNNSNTNTLGGSSSLALSSSMNASLDNSTQNQLFNSLLSSSSILFGGLPNTSFSKLHTSNNGAMPPPGSGPSALSYSNLNNVLHYSGGVGSNSNSNSNINNQQPPPSSPTMSRYTNINSSSRSLFEPQQTVVSKPTTTKFFGSEISIDSSSTPINISTSLNSSNNNNGSYRPIKTSYNSNLYQSSIVNHYANSNSIYTSSSSSTAATTSSQPLQQQPISSSSSNNNNSNDILSSSTTTISNNNTTNSTSTLSTSTSPLYTSSTSSIPSYTSSSSIWNLLYTNLLSNFPTIDTSTLSKHQDREDGLLIGSFINKFTLQSIRDVQLDSEIELECYPNSDKVRFMISNIPIGEKSIQWLSYLIQKEYIIGKTKLAMVQECKSKKDQLLLIDLETKIYYNHHKSTKSIDSLPATVKQHWIQMGQSLNILTNTNTSTYSDISIGNTFLSISNSNLYSSNNGNNSTMPSLSFTLPNNNNSNNNNSNNTFEVVSPMRTSKEMDGGGSGATNNISVDFLSATRSFNTNTTALKLSSGPGGGAMGDAGGGNGRELGKSVDSLFKQRIEEIYTTTVKAEDEEEGDQMMAPSIDSPVQLKLELRSYQKQALNWMYKREMAKPEQSITIIDIEGGNKDLNFIKGGLLCDDMGMGKTIEILSLICANRPSTTTTTTTTSTTPQQSQTPVLGSTQPLNLIQSNATLIVCPVSVLQQWYSEITNNTEPTLSVYIYHGQNRNRDPNFLSSFDVVLTTYTTLGSEYCDSEHNENNNQQQQQISSLSFQLHPLSNTLSPFDLLNNNNPSNSSIISPNNHSPPTTPTGANSISSPQFPVSSSGNSNNSNSTLAPPANLSMSTPSSPNSLSPNTLKKRKRNISISFDVDTTSSSKLTSLAHSNGTLPLHNSMDTSNSTVVTAVPTVPTVSTSPSTTSLSSNLTTPNTSNNNNKQGGVYAVKWFRIVLDEAHTIKERSTRSSKAVFSLEAQIRWCVTGTPIQNKLDDLYSLLHFLRVEPYYSYSWWNQYILKPSKLRDERGFQRLKTLLSKILLRRVKDQKINNSPILNLPSRVISIRHDSFSKEEDEIYQELWGMAKSKFNHLFQTGTLLKNYAHVLELLLRLRQVCDHPLLVEKHLKQQRELLEQEDDVVSQQQQQQQHKQQQKKSGEMEFTTLLEFIKKNPNTYTPLEIGKKLKKILGKGIKDQECTICLDRLETPSITHCGHIFCSNCINSNLISDSPNNNNNSSNSSNSNSFSTPVSSKSTPKYKSNPLKMSSNCLTVTSPSTSPHSTPPLSPKLPRISISSPSPSTDNLISAIHSISNNSNNNNKVESKYFSCPICKEKNMENDIIPVLFESVRQLEKSNSSNTPKKVVDDSSWKSSTKINSMMEEMNKILKNEPDSKCLIFSQWTMMLDLIEIPLKRNDVGYVRLDGKMPQKQREIAIKKFKEDPTVKVFLISIKAGGLGLNLVAASHVFLMDPWWNPATEEQAIDRVYRIGQNKNVNVIRFLIKDSIEDRILKLQENKKNLAQDALQLKKQIRIEELKMLFSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PY42</accession>
    <name>A0A8J4PY42_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CD-NTase-associated protein 12/Pycsar effector protein TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003659</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000117">
        <property type="protein sequence ID" value="KAF2075049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PY42"/>
    <dbReference type="OrthoDB" id="18159at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050135">
        <property type="term" value="F:NADP+ nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019302">
        <property type="entry name" value="CAP12/PCTIR_TIR_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10137">
        <property type="entry name" value="CAP12-PCTIR_TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="CD-NTase-associated protein 12/Pycsar effector protein TIR" evidence="3">
        <location>
            <begin position="203"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="323"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="360" mass="40993" checksum="745842447D8CA8F3" modified="2022-05-25" version="1">MIICNNWRIDYLNIYTHFLFLLLLTLNINALPSHDSFTNNRVSHGYPVRSGYTLLCYSCNSATCANNYTLFTSDSNDSHRNNTPSCIEIKLLQCSILYNPCLDKYSNAILKSENSSIIVKYFQDKQCKIQLTNTNSNNSSEPNSIKQNCSQCYPGMQEYICHTPSNNTLPPIPVTIPKTPINTNNRVIINDTNGNRNNLNKTKVFVVYGHDLEARDNVERMLIDMNCLPIILDKIPSGGAMTIMEKLEENMDVEFGIVIMSHDNHCYVKDEATGHLVPSAYPRLNVVFEFGMLVGRLGRKKTLYLELGSNTASMMSDIAGVNYIRYKDDLQNLRLEVTNLLNKNNILKILYIYGKMIYIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PY83</accession>
    <name>A0A8J4PY83_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003559</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000113">
        <property type="protein sequence ID" value="KAF2075124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PY83"/>
    <dbReference type="OrthoDB" id="20336at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="262"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="295" mass="33839" checksum="4C7DAC9961C7924E" modified="2022-05-25" version="1">MLSRVCKYSLTSFGSRSSGLFKSHRVALHNKIVLSSVRFYSTEKSNNNNNNSNDTTTTTANTNTTLSTDSVVAQPTFNVDLSKYKTNETLKDMNPDHLVYKSSWENTLAFIKYFLTGQTVISISAPLIALYNKLEPTTVLFLTGFSVWGVIFQAMGQRMMSFFVGRLYRNPDEKFIRVVQYAGKTNVLKIPTDEIQQSIIGGRTKPYMLLSNGAIFFFEKNGILENPEEFENILCGQEYLDKVKMKEDEEYKRLFDEIGSDQSHLKQTSEDLDRELRDLEKEIEQQDQNKDKKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYD7</accession>
    <name>A0A8J4PYD7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rab GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_003009</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000089">
        <property type="protein sequence ID" value="KAF2075695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYD7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001447">
        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2075695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="34806" checksum="1A7C51F4E0CBBD5C" modified="2022-05-25" version="1">MKIKIQYDQEDETEIDSNITHPLKNKIDQNDGDQDLLVTKQDEYDPLPSSLKILIPSSIKSVCFSLDYKSYKSHVTVLDKDDYYSKTPTPPKIRQGLVRKRKRNIPKVFNTWWKPIRKSKTLLRYVDNIFENHITTNGVDFRTKYIDYLDPKYGLTLQIWDRYRGCERFRSTPQTYYRNASAIFVLFDVTDRESFNYAKEELKGIPHTSECIHEVYIVGNKIDLESKRVISREEAIQFAQDNAAQYYEISAKENIDVHQMFDEVGKALFYKKINDPDFSNAKPTPSAPPPKSYCIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYI5</accession>
    <name>A0A8J4PYI5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">T-complex protein 1 subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_006972</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000347">
        <property type="protein sequence ID" value="KAF2071711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYI5"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03337">
        <property type="entry name" value="TCP1_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000073">
        <property type="entry name" value="T-complex protein 1 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000085">
        <property type="entry name" value="T-complex protein 1 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000005">
        <property type="entry name" value="T-complex protein 1 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012719">
        <property type="entry name" value="Chap_CCT_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02344">
        <property type="entry name" value="chap_CCT_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2071711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="532" mass="58975" checksum="CC778B4172A0B596" modified="2022-05-25" version="1">MNSPVFVLNTNAKRENQKEAQQGIFLAVKAVASIIKTCLGPQAMLKMILDPMGTTVVTNDGNAILREIDVTHPAAKSIIELSRAQDENVGDGTTSVVILASEILASSEIFLEKKIHPRFIIKAFRMALDDALAIVDQYSLKIDTKNRTELLKVIQSCIGTKFISKWGDLMCGIALDAVQTVHIQDPSDSSRNEIDIKRYAKVEKIPGGDITDCKVIRGVMLNKDVTHPKMKRMIKNPRIILLDCALEYKKGESDTMVDITNEDDFAALLKIEEEYVQKICEDIIKLKPDLVFTEKGVSDLAQHWFVKKGITCLRRLKKTENNRIARVSGATIVSRPDELQESDVGTECGLFEIRKIGDEYFTFLEECKNPKACTILLRGASKDILNEVERNLTDAMNVARNIVLDPRLVPGGGAIEMALSQALIEKSKSIEDLHQLPYKALAQSLECIPRILAQNCGANTVKLLTELRAKHATNPSENYTFGVDGEKGTVVDMKELGIWDTHAVKVQTLKTSIESACTMLRVDHIASAVSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYW0</accession>
    <name>A0A8J4PYW0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003191</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000097">
        <property type="protein sequence ID" value="KAF2075499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYW0"/>
    <dbReference type="OrthoDB" id="20420at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00102">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052595">
        <property type="entry name" value="LRRC69/RLP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48057">
        <property type="entry name" value="LEUCINE-RICH REPEAT SERINE/THREONINE-PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48057:SF19">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING N-TERMINAL PLANT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="953"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="700"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1040"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1040"/>
            <end position="1061"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1090" mass="122940" checksum="C42F63CBB817387C" modified="2022-05-25" version="1">MIIYSNNKCITQAGNTRQHFILLRIYYDTQGSTDWINNTGWKAYTETLVSSDNYVSYSMNSINPLTGTLPYYLWEEYFQAIDKNKTLNDIISSKLKSKNIDICNLYGITCSDTLDLVKIDLSNNNLTGQITSQLSFLDTLEYLNFSNNRLDGPLPTGLFSLESLKYLDLSHNQITCDPFPPVNSPNLISINLSYNKLKGYFSLSWKSPMLEYLNLSFNQLSGTIPNPLFKQGKLRKVDLSNNKLIGFVPLLSKGIISHLNISHNQLIGSLYINSLWKTGYLEVLDIQDNEFTGYLPQTILDYAPFKYINLLGNELLGTIPSSIVCDARITILTNLAPSHCTPFITKVSTIDPLGGNVTISGVDFGMRDPIVVKFSDGTICEKPIVTRPNSVIRCKNPPGRHGKDIYVTVFNQTTKLDLDYHVPVVESISRVPIGVGGKVTISGKHLTSFEYAKVQIEDFIANDTVVSDTSLIFTIPPNANISIPATLNVVIDGLQANVSDISFYYTSVYNPDIRVSSPQSYTDHIYVYCTTLPMKDLAEIRINSKPCLNMRIINESSAVCVPPQNSYGSNNKVGLVFPNMAPQYTKTLSYPAPIIDHIPGLSMTSGGILTIYGRNLKAKRNQISVTIGNITCCPLNDEPDDYILCYYPAVDKENIIYNQPAEISVQNQTVVLKHAYTNTDSVCEDQCIQHSTARCRGGRCECLPNFTGPLCERDIPDTTIEPYPDAPGFKYHYPEYISDYVFTVQLHRIELIENDEILDTSGLQWTMEKTNTTDTIYTAKHNDILINVRLFRPPKHIIMFYGGEEVEVPRNNGRFIVDLKNENNLSSANYSLNMHFWASTIQDPNYICPPAADVRISSLNSWVTIGISDIVLYHRITQRIDLDGLVFPMNSKIISSNSNDRNTKILYSSSQFQKHLNFQCDFSTLMAKNEVLSKKDGGCYTDTNRSFPDRGTIVFVIILSIIFFMTIVIAYCSHLESRKRKKYIKAFEKKEEHIPFLATPSLFNQPYFNILEDKIEPIDLYPLHRSKSISLNQDSDYNSDYIYENNSNNNDNNNYNNSNRNPMHDGAGDLSEFGTTLSNSYSSNISHNHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYZ9</accession>
    <name>A0A8J4PYZ9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycosyl hydrolase family 13 catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_003097</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Amyloplast</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000093">
        <property type="protein sequence ID" value="KAF2075592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10263625at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03791">
        <property type="entry name" value="GT5_Glycogen_synthase_DULL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013534">
        <property type="entry name" value="Starch_synth_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45825:SF11">
        <property type="entry name" value="ALPHA AMYLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45825">
        <property type="entry name" value="GRANULE-BOUND STARCH SYNTHASE 1, CHLOROPLASTIC/AMYLOPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08323">
        <property type="entry name" value="Glyco_transf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0035">Amyloplast</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="6">
        <location>
            <begin position="1165"/>
            <end position="1571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="24"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="82"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="302"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="524"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="721"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="82"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="302"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="341"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="380"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="525"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="607"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="634"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="647"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="721"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="731"/>
            <end position="748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="2301" mass="256543" checksum="0321915C5C26C068" modified="2022-05-25" version="1">MENIEELLQQLILQDDTYQLEKLGVDDEKKRSSQAASASASSTPPSGVKLNISSPSSRDEFNKVKVPISMALLDHLNALKSLPSSSPSSPNITSLSSPPPSTSQQQQSSTNTSDKNSSDDKSNTGTTTTTTPASPSVEYDKKVALINELVEKIISKYITPTVLYDAQGQKISSIDDDLTIDFQFPFNHDENPLVSDILNSYDTKREEEEQEEAMALNDPSRSPSNVFGPLKVKSESNASHRTCYRVNWKSLIVKLHQSILLSGLYQNLLKKISNENTSSQLDSSQTLFQTLKDPKLSKQVSLSSSSLLMKPASKEDPGVESNKNSPKMASAAAAVIEETRSTPTTPGNTSPMLKKGETNILDSPKKTNISGNAPGTPGKSSQQQSVTGSSSSSNFSLSALMAGQTKDAPPALKELAERSMKIDQNKFGIQVPASSSQPPMYPNNPNMNKLPSSYETDPILLPDGSEFSNARVVLPSTPTSSSASSSIPIFNVGSSGAPTSLAQSPSPSSMPIQMPASSMPIPLSGPSQSIGSNLFSPPTNPLSTSFGAPPRFSTSPYQTQPQQISSGMPPTRLTESFFPTSSSRGPPSYDGNVNSTPTIQIPQTQNLASSSMMSVSPSPSSLLTSNPLSLSGSFNRPSSMGTTNQFPPNIELNPISINNINSAPTQQQQQSNASSLTSMTASPSNIASGVNVLNLSGNVLPQQPIPQVSPQMPAIDTMVPASSSFKATPPQSSSASSSANGSAVSNQSKSSTVQSLNKPITGLSDVQFPEWLDITIYWNKSNNYLLVDENSAAEKDISNGINIEVRSLILAERSGGRLDHLLEAKLISLINQHLLSIIESKTVPWIEAIYNNWIKSVNVHYFSEISRKLSSTVTTFNSHLWNSRKYNAFLDRIVNLKRHYPGFKTPQDHVQVTSKGAPIPLQLTSQKEEQEMKKIIKQLEQDILYLISIQHQSSLKHLERLLIGLMGHTSTEARQSSVRLLNVIYDSHSWQFDEPLKPVIRVIGDAFHVEIELESPLPEEVLKRVHILTVVPSSQRISNLIHNSSAAGTQSIKTKQQHRNRYLHGVNSTLFTRHKPKVEGNKLYVDLPSFYRAGYYDWRIVELTSNGTYVPFDYSSFKLKTLASRITSPQSNSATTANPDASSENEFIQGRFIVHPKLREEIMHEVWVDLNDSVWDPSRGDMVKKGNLQNLIDSLDNFYQDGVTTLYVMGVLEKLPNNQPLCPTDRDLPNRSLGGTALFNQMMNKAKQLGIKIVIDSTTRLSSKAAHRKYNGVICHTLDNNGQLCRLEGTDSSEFTWSDCVMMNMRKQSVWEMFVQETLLWGERGVDGIRIDSAHTCPLIFRSNLDELYRLDSDDLPHYSNQQIFEGEIVMKPSEEGKNFGYWGTTPPSPKHSTPFYPNPVFIKMTREVWNRFPQFVFLAEVYWERELHSIISGLVPYSSAIPKALASVFDKGISKDGIVFDLRQRNSVKTLYDYYEMRMSTYPQNSIILFPSSTHHSPYPLSIYNAGAWAAVDMLYFLPEIPITYIGEQFGWYYHVDIHTSRVKVGMSSTPYQLQYPQIRGHYAHRINLRKTHALLRRGGMIPLLCYHQNGWHDRVFGFARFSGNEIAIIAINFDNHDTEFYVDCSPLAKIATQDTIYRMVDLINPNNPPQYLSLNEFLYEKHFVLVPGYSSICWGVYHQVSSPAAMRVLYEHSMTRLKQLLADDVDPSNNLIYSLIKNSIGNVNDFGKALTDILSNLSGHTEKTFSSFIQNVLYHLTRTHMEGYKILSYLEVLSKRTNIQRIPSICKETLQHNILGPIVFITPEIGRFSTVGGVGVMLDELTRSLQLLGCEIHIISPYYNYGKGGRTGYLEKEEGIKWKRNVLTYVGPDRVEVGVHEGIDKGIHLHFLHHFEYFPTPYNAGSPIHQLKTIVLFAKASLELLCQIRVLPSVIVTNDWFCGLVPGYAKSGAFGTTFNGTTLFHLVHNLEEAYQGRIYPDGGQDDLNWIHHLHRDLIVDPSCNQLCLNASRCALLTSNNWGTVSKSYLYDLLRTSHLANLLHRFPQAFAHSNGIRVRERKELMEKVSKDHWSAKEELQKKYFNSVDRTIPVFSFVGRIVLQKGVHLILNAVRELIQYYQGKIQILVGGMANMKDPYAASCAWSMQALCKQFPNSFWADPDSFFSDGPLVNLGSDFGLMPSLFEPSGVVQQEYFVAGTPVIAFKTGGLKDTVFEYSTTDESGNGFTFEAHKHTDYVQAIHRSYNIYQNPHHYQTIRQKASESVLDMSVVAEAWAKEFSRMRRSIWADQNLVQEYSQKLLNDSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ99</accession>
    <name>A0A8J4PZ99_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mitochondrial escape protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001950</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000050">
        <property type="protein sequence ID" value="KAF2076761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ99"/>
    <dbReference type="OrthoDB" id="10267654at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039627">
        <property type="entry name" value="Yme2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32198">
        <property type="entry name" value="MITOCHONDRIAL ESCAPE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32198:SF3">
        <property type="entry name" value="MITOCHONDRIAL ESCAPE PROTEIN 2 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="540"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="648"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="540"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2076761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="730" mass="83217" checksum="B8C73CD80F95CE6C" modified="2022-05-25" version="1">MISAYQKLIKPQSAIIPATSQLLPYYGLGKKRLGLQNNSNNINNNNNNKSQQNPIIPLHYKEPIAPGNNANVSHLSMNTIHTNNAIGNLNKRNINSWGFRFILGFSIIGIMFFARRWTPRQIKRIITKFKKGSDDVDILSLTAKEQRMYTNISFNRDREEESLNKFLQSPPLGPIVVVGPEGSGKSHIMKKILSSRSMSILVDMRQNAVMSGDELILSFLKKLGYLLPSSDPISSLLFKGDKKQKINVQEIVQGLETVYLSLIQIREVYNITPLIAISNIEALPTSENFTRFLDWCLSVTDNKLAHIVFLTSSQFVHFHLDANINFRKRRFVYNIKFPSNEEVINYLHLVFNESGDSNSSSTESSVNSHLNLLSTNNNTPFTSAKNNNIIISDSSSYFGKKCSLSKPEIEHMVSIFGGQMRDIDLISGLVKRGEDFHYVMDLFISETVQKIGSQIDSMFQKANIADNDTIKTNTYEKYQRFYKMLEILTDVHLIRVSELIQEVFHEQPEELDEYFQDNLIYFWVGATSHHSIPEITISATSSSNSTPIIDSSSNSNNDDIDDDETRGNVEKKLKGSAGSLSMISSNANNSASSANNGGLGNNGMNIRQYSFDNFVSFSSPRIQHAVQIVLKDNRFKLQRETIEKYFKKSDLKDDKKELEEEKVLKHNEYERITTRLDNLVANSKEWIKYIGEPEFLKRKDYLILKENELLLQMEKINSQLFKIENELDNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZC3</accession>
    <name>A0A8J4PZC3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidase S54 rhomboid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_003411</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000106">
        <property type="protein sequence ID" value="KAF2075282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZC3"/>
    <dbReference type="OrthoDB" id="10257275at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000008">
        <property type="entry name" value="RHOMBOID-like protein 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066:SF1">
        <property type="entry name" value="RHOMBOID PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="93"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="162"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="10">
        <location>
            <begin position="53"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="261"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="264"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="296"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="307"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2075282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="324" mass="36238" checksum="D94BD1A600A99454" modified="2022-05-25" version="1">MENVIRDNLLRIPLVTRSFLILCVSLFVLTAGVKVLAVRDVCFSPYYFSSFRHVYTIITSSYFHINALHLLFNMMSFVGLGSNVEKAKCGSLLFLYLVLFFGIFIGFMILGLSYLLFTLGFNTAYYSCHIGLSGIVFALLEIECYGDYNRRLFASINIPSKYYPLVVVIITQIIFPQASLVAHLAGIFVGYLYVNGYLNIMIPSTRLQNIENHGYLQYNGYITLQQNVLPQFDSTSQSTGLPASLSNFTSRVSNVFSSQGRQLGMNNIGDSNNNNTGSSIESIDNSNNEPYVPMTMNYSPSVDMSLNNPNNTNSKDNNNNNLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZS6</accession>
    <name>A0A8J4PZS6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_002230</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000059">
        <property type="protein sequence ID" value="KAF2076490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZS6"/>
    <dbReference type="OrthoDB" id="10252009at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF525">
        <property type="entry name" value="MAP KINASE PHOSPHATASE WITH LEUCINE-RICH REPEATS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="9">
        <location>
            <begin position="540"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="10">
        <location>
            <begin position="613"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="96"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="183"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="96"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="107"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="145"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="194"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="239"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="255"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2076490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="692" mass="77036" checksum="F3C972B587C455F9" modified="2022-05-25" version="1">MGNTNSTNNGGNNVNNNGNQLSSSGGVGRYRVQIVDLNGKLHIDYRNRNMKKLKSTMFSNFIDASYEIVSADTLTNSDYMKNKPNKVVPAYSKSNSQVQLPTGQQNTTTTTTVTATSTISSSSSNSTINESATTDDHLVNNTFESSSPVPSPSSSPSKKLGLRMQFNPDLTIEETIKVLEEQELEKQEQQQQVPNSEGDVSNHNIVISSEEKELDGATTKNSTDSISPLKRSASDNKLKTNNNLAILNESQNQRNSVNIIPTTTTTTSTTPLSSSPNTISKSSKFGKKESKKQSSNQSSHRNSPNVLDLQNLKNQLDINSVDLSINRLENIQPEILKIMNALNVKEISLSTNFFQIIPDFGMVKSLQSINLSRNKLNKFPIQTFLELPNLSNIILDRNSISILPEEIGKLTSLKYLSIRNNSIDAIPNSFTNLFQLVTLDLSNNRIKFLPNNFEQLLSLRMCWLTNNSITMLPSMKKLVNLTIFDVASNRLTSIPSDLAYLSKRESGSLIELNIRDNKEIYQLPLEFKTLETGFTLTTSLPTEIIPGVYLGGLDSANNLGILQALNITHVLLAIGDLQPCFPKFFKYYTIDDARDTPQYDLSIHFDQTNCFIEQGRTTGGVLVHCRAGISRSSTLIISYLIKYHNLSYQKAFEFVQSKRPQVQPNSGFKEQLLKYSSKILYNNQISEQTLGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q059</accession>
    <name>A0A8J4Q059_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000233</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000004">
        <property type="protein sequence ID" value="KAF2078483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q059"/>
    <dbReference type="OrthoDB" id="1055097at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="525" mass="60834" checksum="BDE818BA3E8935BA" modified="2022-05-25" version="1">MKAQLFYSVFRNIYLTRLIRNQIFKDQIIDVVDLQYLNHNHVYLSSILDKGSSVFVKLKIQRNEFHQYKDNSYYYMINYLVLDLKQDESECFDLTLVPKSVCRLSIIIDQHTQVNGILPDTITDLEIKSVNILDHSFVVESMVSKLPKNIKRLAIPYNYRFKWSGPIVLPLSLDDFEYFCLYQDAKRLVIDKSKVFASAQVQVNRIEELNWVYGQKWVQNIHVRGCPDGMLGSQTPGHIKRMNILNYKPDTIHKDFLPRGLKHLQLHYLGSLSEDFLPPSLTYLKVSFFNQELESNYLPGHLKTLYLLNFEKPIRCSVLGLSVFPPGLHFLYLPKFNQPLTMGCLPESLAVLKLMSYNQPLGPFVLPRQLKILELDSYTHPLVNDCLPSCLTHLQLPSFKGSFESIGPLNHLQSLTVFKLNHTVTPTISNVKDLQLMFDEIQDWELEINLQNTSIKRLAIFITGKDRFLSRNLLPFGLVKLKINGFKIETSEIIPTSCIYLETDIIDINPDLIPASVKYYKYRCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0M3</accession>
    <name>A0A8J4Q0M3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Rab GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_001694</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Lipid-anchor</topology>
            <orientation evidence="7">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000042">
        <property type="protein sequence ID" value="KAF2076985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0M3"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01869">
        <property type="entry name" value="Rab1_Ypt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000359">
        <property type="entry name" value="Small GTP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057289">
        <property type="entry name" value="Rab1/Ypt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="196"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2076985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="23018" checksum="8C4C0ADF4B057076" modified="2022-05-25" version="1">MNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTINLDGKIIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDHVSFNNVKQWMQEIQRYACDSVTRLLVGNKCDLIEKKIVDTSTAREYADSVGIPFLETSAKSSANVEQAFMIMASEIKKLQGGISSNNSNYNNSVVKPSQGQPIKKKKSCDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1C6</accession>
    <name>A0A8J4Q1C6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">AN1-type zinc finger-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">CYY_001284</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000029">
        <property type="protein sequence ID" value="KAF2077435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1C6"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18044">
        <property type="entry name" value="DEXXQc_SMUBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001146">
        <property type="entry name" value="DNA-binding protein SMUBP-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.50:FF:000002">
        <property type="entry name" value="Immunoglobulin mu DNA-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1110.10:FF:000013">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050534">
        <property type="entry name" value="Coronavir_polyprotein_1ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004483">
        <property type="entry name" value="SMUBP-2/Hcs1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048761">
        <property type="entry name" value="SMUBP-2_HCS1_1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00376">
        <property type="entry name" value="IGHMBP2 family helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788:SF8">
        <property type="entry name" value="DNA-BINDING PROTEIN SMUBP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01424">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21138">
        <property type="entry name" value="SMUBP-2_HCS1_1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00393">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="R3H domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="R3H" evidence="18">
        <location>
            <begin position="727"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="domain" description="AN1-type" evidence="17">
        <location>
            <begin position="894"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="671"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="790"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="952"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="680"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="694"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="791"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="808"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="828"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="844"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="854"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="952"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="974"/>
            <end position="983"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF2077435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="983" mass="110751" checksum="8283547DEE7ADD72" modified="2022-05-25" version="1">MTEIHDYVKNALDLLDIEKESEVNEALNNFSTLSNKELELKGVTVRKLKLANVSTGLNGRSLLKFQSATLNSEIPPHKITPGDIVGIRSSKSKPGTSHFTKGIVYKVDSHKIVVTIDDNDDYQKNDIELLLESSDSLVFALDKLANDVTYKKIKDALDNLKQQQTTYSSQSRLLDILFHGKEPLNNNHFSTIKDFKPFSKSLNTPQLDAIKFSLSSNDIALIHGPPGTGKTTTVVEFIVHVVKSGKKVLACGPSNLSVDNMLEKLLNYPDFVVPTRIGHPTRILPTLIQHTLDYKTKNCENSQVIVGLRQEISQLIKSLKSSGERKSVQSQIKALRIDLKTRERQLVHQIINNSNVILCTNTSASDSSIKGKNDFDWVVIDEAAQALEPTCWIPILKGQRLLLAGDHQQLPPTIHSTEAASKGLSVTLFEKLIKTYGEAVSRLLTVQYRMNHFIMDWSSKEFYHSRMIADQSVSQHLLFERDNSKCLIKQTPSTTCPILMIDTSGCDFEESAEAEGESKYNHGEAVLVKKHIERLIDSGVKEKDIGVITPYNGQVKLLISMIRKPYPLIEIGSVDGFQGREKDVIIVSMVRSNPAPHKVGFLSEDRRTNVAITRARKHVAVICDCDTISSHAPLARMVDYFKDMGLFRSALEYEYEDFIPSEEDEWIPDEQQDMDEKDSSGSTSTTTATKQKKSKFELNKEKRLKKNLEKGKQKDQDQSVEKEQDKEKKRNQINGIIQIFLNSNNQTHTFPSSLSSFERLLVHEFAEKYNLSHQSVGENDQRVISISKKSTTTTTTTTTTTTAVENKDQDEDDDEDEENDQDSDTESNKTNANNNSNNSNNTSKKTKKKKKKPASNNNNNSNNTKNNNNQKTTTKKTKTADELLKEFESLEFREVDLTTCGMKNCGKNVEILGRVCQFCTRKYCTQHALYEIHGCGEKAKFKAREDWFKQNADKKHNTAASERAKKAVEDAQNSRKKKAPSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1J2</accession>
    <name>A0A8J4Q1J2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RabGAP/TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_001632</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000040">
        <property type="protein sequence ID" value="KAF2077065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1J2"/>
    <dbReference type="OrthoDB" id="17687at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000002">
        <property type="entry name" value="TBC1 domain family member 9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF20">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="4">
        <location>
            <begin position="656"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="990"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="1026"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="273"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="427"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="381"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="429"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2077065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1120" mass="128842" checksum="CF6F7998F04C2A48" modified="2022-05-25" version="1">MGWIKPRSIVLRKAWVTLEENQYFQLLKRNSSIPLFNDLISKINESSDYSVISEYKIALKNKENHMIGVSNEFREIQAMWRWIQDNVLPSMLEMNNSEIYEFLSLKFTCMGIEQHDHKEIEKEKITKDRYVLKREKSFRKKFDLGQTENLITQYTCSLDKKHGCLYISENYISFHSRIFQTTKISIPFSNILDMKKNSTALKMIISSIKIQTTDSKEYFFHTFFKVDETFQILDQLWKFTMNKMLFNADLNSKPSLSCSGSYLPSPSINIVSPNNNSGSNASNNNSANNSSQSNNNNTSNNNNSLSSLSPTIAFSSSFNNSLKSSGSYFSTFIDISNVSTSYNPTPLALSPSHSSPSPFETHLMFNANNIATTTSGEYNIPSSPSSPSHSLRSSLNGSTGFPFLSPSITTPTPTSLSSSVNNLQIPIVNNTNNSSNNQSNSSNQNERNSIQNSQNNIQPTVKDLLNNQKRNEDYQSLFSLPSTEILIEEFLASICKINYEMPGKLYLSQRFLCFESTDSLLVIPLREIRNITNEKSLNLKDNTMKIITLGNQKFYFYSSKIDKLYEIIRQVWFELISSKNQNQNNNNGSSGPVNQNNNDNYQIIGFTKELIDKEFSSNYQQKQNNQLLFWEQYMAFNGEDVAMFKSYELKSLIRQGVPDSLRRKMWLLCSGALYKSYCYPEDYYQNLLDSHKGETNSSTDDIEKDLHRSYPKHPFFREKKGQDALRNILVAYSWRNPSIGYTQSMNIVGAIFLLYLKEEEAFWLLCRLCEDLVPDYYRPGMVGSIADEKTLENLLSIYLPSIDSHLKKISCPLSMIVLPWLLCLFIGYVPIELSLRILDCLFYEGNQMIFRVGLGIFKVCQESILDCKNAEDVMILLKSISSSSSPINSTPNGVEQLLNVAFTNFDILPLDKIEQFRNSNRIMAIKSMQVVNKKSKIREWMEKYNLDRADSEKVYDYFQSSTSLNTSKFGITQAKFNELCRDILPFSWNNRGDIIQLIFRVVDKDCDQLISVDEFIDMIVIILKGTHLERLKFCFEMVDQDADNIINYLEYKFLLEIYIGLYNSNSYSFSLSMEIEAFVSQLQEEGEEEEEDQLKIDLVESHLSEIINRFGIHTNNIYKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1R8</accession>
    <name>A0A8J4Q1R8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">CYY_001463</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000035">
        <property type="protein sequence ID" value="KAF2077209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1R8"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06609">
        <property type="entry name" value="STKc_MST3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000411">
        <property type="entry name" value="Probable Ste20-like kinase Don3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000092">
        <property type="entry name" value="Serine/threonine-protein kinase 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046409">
        <property type="entry name" value="PDC10_dimerisation_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048288">
        <property type="entry name" value="PDCD10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF10">
        <property type="entry name" value="FI20177P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20929">
        <property type="entry name" value="PDCD10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="12"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="284"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="323"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="339"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="356"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="405"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAF2077209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="495" mass="53631" checksum="D07C751C4B0CCB60" modified="2022-05-25" version="1">MASKKGDPEELYIKQEKIGKGSFGEVFKGINKKTNETIAIKIIDLEDAEDEIEDIQQEINVLSQCESAFVTKYFGSFLKGSKLWIIMEFLAGGSVLDLMKPGPFDEGYIAIILRELLKGLEYLHSEGKIHRDIKAANILLSANGDVKLADFGVSGQLTDQMTKRNTFVGTPFWMAPEVIKQTGYDSKADIWSMGITALEMAKGEPPRADLHPMRALFLIPKDPPPTLEGNFSKGFKEFCALCLNKDPNQRPTAKDLLKHKFIKAAKKTSCLTDLIERRQRWLQANGNSMDDNEEDLDKDGKGNEEDDGWEFPTIKQKSPAPKPAVTPVAAKEPVVTPPSTATKPNTTVTLSKDPIVSPSATPSTPTKTAPVTTTTTTVTKPSTTTTATTPAATTETKPRSSSNSAGTTPAPAANNAVAATTPNGANPANPVNGAPRASALTSLIYPVLSKLLHNTQDENVISALAQLKMAFDNAERAKPGITHQLIAQIFETLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1Z6</accession>
    <name>A0A8J4Q1Z6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001332</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000031">
        <property type="protein sequence ID" value="KAF2077329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1Z6"/>
    <dbReference type="OrthoDB" id="5425247at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="442" mass="48761" checksum="44921A1FC770A07D" modified="2022-05-25" version="1">MDTKVRINHPDDLCKVTGNGFGKLRYIRPISESVKHHRELFKSTGQFSTTQPASISVAAPPQLLLPKSVLPTYQKNDWKGIYQLQQQANKDLVPIMSNFQNHPDNSWIEVQDDTDPSQFYYMSWDNQGNYAWCWCSAVKTASDPFNPDIKLNAPTQYGIFSKTTSIAGIHTYNLGLKEIVPELVAASILSFFINKAIKQGINFVASDLTKMLGDAAAEAGCELVFTISSFVLGAVCSCVVFAIVFIGISYLWNFLNKRFQICVSVYNWDTANDWQLPVQSLSNAVNPGEDQTNNLNINIAKQIPPGSNPFGNGVQTPPDIGSELMHTVDAAMNYAYIIYVNKSTFMQGLSFALKCTKNNTNTGFSYAFQCPWTASNGHYIENTPQDPNSFLSNARSHWVSSTAALTTTVAGMPIRCYIDALTGGDADGDDSYQVAVHIDPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q274</accession>
    <name>A0A8J4Q274_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2804 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001909</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000049">
        <property type="protein sequence ID" value="KAF2076782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q274"/>
    <dbReference type="OrthoDB" id="4763727at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021243">
        <property type="entry name" value="DUF2804"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35868:SF1">
        <property type="entry name" value="DUF2804 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35868">
        <property type="entry name" value="DUF2804 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10974">
        <property type="entry name" value="DUF2804"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="363" mass="41595" checksum="0AB7939413A92EC6" modified="2022-05-25" version="1">MSKHICYSDEDLKNQIEINNCQELLNEKGCLNVKGWSRQPCLKYNPNNLSRFKKAYRLKEWNYYSIGSNSFYFAVAAIDLGYINNYQCFFIDFSEVDSKPVHDEITSSAAFSKGLMLPKDSCEEGIHAELKTKNFNLQFSVAHQNNGETIKHLIKINNAKMKLVADLEFDLSPNRESVVLITPIGEENFYYNRKTNLIPINGTLRIDGKEMLDDTCFGIMDWGRGIWDYNSFWLWCSAWGKDKKNNTPIGLNFGSGFGNLSTHTENAVNIDGVIHKLGHVDIKYNELNLLEPWTFHCKHGRFGGNPLTFTPIKKKLESNNFLLVMSSFHQILGKFSGELVLDSGEIIQIDDIYGFVEVHKARW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2B2</accession>
    <name>A0A8J4Q2B2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Zinc transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_001289</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000029">
        <property type="protein sequence ID" value="KAF2077440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2B2"/>
    <dbReference type="OrthoDB" id="9944568at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050681">
        <property type="entry name" value="CDF/SLC30A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11562">
        <property type="entry name" value="CATION EFFLUX PROTEIN/ ZINC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11562:SF17">
        <property type="entry name" value="RE54080P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="Cation efflux protein cytoplasmic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0864">Zinc transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="181"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="329"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="358"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="11">
        <location>
            <begin position="148"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein cytoplasmic" evidence="12">
        <location>
            <begin position="391"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="280"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="472"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="35"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="81"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="96"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="487"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2077440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="504" mass="55369" checksum="E99D612CE7E4B49E" modified="2022-05-25" version="1">MENFQKSNHSSRNNSPSFTKLSNNSILEEITIPDNDDHDREVVEEHGGGGSDHSHENDSHSHDSHDDSHEDDGHHRLQDLASASSNHSNSNTNKQGHSHGGGKKKKHGHSHNHNDHDDHDDHDEHDHEGENVPLFKSMDDKKKARYSLILALVLTTIFMVGEIIGGWVANSLAIMTDAAHLLTDIGAMLLSLFAMWIANRPPTSSLSFGFHRAEILGALASVLMIWLLTGVLIYEGIQRIIHPPDSVNGKIMFIIACGGLFINILDAIILHWGAGGHGHSHGGGHSHGGGHSHQKKKKQAHSHLIGEEDPENKVDATKKSVRNINVHSAYIHVLGDCFQSIGVMIASALIWIKPNWKIADPITTFIFSIIVLMTTIQLLKQSLGVLMEAVPSDIKVLEVKQDLDQIPGVSEVHDLHIWSITLGKPALSVHLTILPDVDSEVVLQMANKILYEEYSIKHTTIQIEKPTVKDACKDHSCPPPKAKKRSSVASVSNNNNNNNNPSSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2L8</accession>
    <name>A0A8J4Q2L8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thymidylate kinase</fullName>
            <ecNumber evidence="3">2.7.4.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_001701</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thymidylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000042">
        <property type="protein sequence ID" value="KAF2076992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2L8"/>
    <dbReference type="OrthoDB" id="425602at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:dTMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006227">
        <property type="term" value="P:dUDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01672">
        <property type="entry name" value="TMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000679">
        <property type="entry name" value="Thymidylate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018095">
        <property type="entry name" value="Thymidylate_kin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00041">
        <property type="entry name" value="DTMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344:SF1">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01331">
        <property type="entry name" value="THYMIDYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate kinase-like" evidence="10">
        <location>
            <begin position="10"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2076992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="211" mass="24060" checksum="938262DA4CE5C68F" modified="2022-05-25" version="1">MIKRGVFILFEGVDRVGKSTQVLNLVNHLNTKKDVIVNGSKAMRFPDRTTPIGAIINQYLQNATQMDDNALHLLFASNRWEAKDSICNLLNNGTSIVVDRYSYSGVAYSAAKGIDFDWCFSCEKGLPKPDIVFYLSMDCEDATKRGDFGGERYEKLDFQKKIKDIYEKKLVDPSTWRIINANRPIQEISDEIREIIDKELVELQNKPISTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3A4</accession>
    <name>A0A8J4Q3A4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_005349</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000211">
        <property type="protein sequence ID" value="KAF2073331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3A4"/>
    <dbReference type="OrthoDB" id="10263513at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004977">
        <property type="entry name" value="Ribosomal_eS25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12850">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03297">
        <property type="entry name" value="Ribosomal_S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="10"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2073331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="111" mass="12038" checksum="89FAA945BF3FFA05" modified="2022-05-25" version="1">MPPKEKAGGKSKQIQASKSAAKSSGAKGARKKWSKGKSREKLNNMVLFDKDTYAKLMKEVPTSKVITTATVSERMKCNGSLARKAIKELLSKGLIRQVIKGHCAGVYTKAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3P8</accession>
    <name>A0A8J4Q3P8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA (guanine(46)-N(7))-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_000559</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42708"/>
            <dbReference type="Rhea" id="RHEA-COMP:10188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10189"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74480"/>
            <dbReference type="EC" id="2.1.1.33"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.33" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000010">
        <property type="protein sequence ID" value="KAF2078175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3P8"/>
    <dbReference type="OrthoDB" id="47276at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043527">
        <property type="term" value="C:tRNA methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008176">
        <property type="term" value="F:tRNA (guanine(46)-N7)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003358">
        <property type="entry name" value="tRNA_(Gua-N-7)_MeTrfase_Trmb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417">
        <property type="entry name" value="3-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417:SF16">
        <property type="entry name" value="TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02390">
        <property type="entry name" value="Methyltransf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51625">
        <property type="entry name" value="SAM_MT_TRMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2078175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="308" mass="35665" checksum="707268254BCE5972" modified="2022-05-25" version="1">MFKQITQLSRYKNISCLNNIYKLNSSSSNSRTQPFNYYYTTKTIKTSTTTTVSTQNEHDIDYNSDDNTLKDTKGNDINNSGLNKTIQSIGNVGITKKSSKIRPHVNPFSPVLQMKVHIPDWKYKYQDLSLPFHIDLGCGRGEMLIDRSFSLQDRNYLGVDIRAAFVEVAQADLDQRREKTFDSEKIKKNIDFLTSHVNVNFSHLAQSLPPNSIKEISILFPDPWWKKRHVKRRMLNSQLIQDIINSTPIGCKIYLMTDVASLLVDMDALFESFPNKFKKLDKDIKWPLPSTRAQNYFQTVNYSIFERI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3Q5</accession>
    <name>A0A8J4Q3Q5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Coiled-coil domain-containing protein 22 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_001177</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000026">
        <property type="protein sequence ID" value="KAF2077559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3Q5"/>
    <dbReference type="OrthoDB" id="10266736at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000060">
        <property type="term" value="P:positive regulation of ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008530">
        <property type="entry name" value="CCDC22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048348">
        <property type="entry name" value="CCDC22_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048349">
        <property type="entry name" value="CCDC22_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15668:SF4">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15668">
        <property type="entry name" value="JM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05667">
        <property type="entry name" value="CCDC22_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21674">
        <property type="entry name" value="CCDC22_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCDC22 N-terminal" evidence="5">
        <location>
            <begin position="7"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="CCDC22 coiled-coil" evidence="4">
        <location>
            <begin position="117"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="278"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="382"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="298"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF21674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2077559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="620" mass="71310" checksum="C39425E18BDAB921" modified="2022-05-25" version="1">MHIHLSEIEDNTTIKDLDTDLLYKCILAYLKVINENKVTNLTSTLPKNMSSRVNSWSLIANLIKDLGYRADLSFHNLLYPNINDTRRILIFLGQNLPKKEIDQTGSGGASTVKIEDQICLYLQGCIKESWMPYFCPFSKRVPGNYSTARLFTTQEVKIPFRGRQLKVTPGLEEYFTHFLPPITNQPNRFDDLAPSVFEYNLSIYAEAQERDLEWKRKGSASGLNPIEYKKQKLKNVLNKMNDSIRTSMMESGGMSEGSRGGRLESLSELISDFRGYSEDSEGQFSRKKDFTNELSNQSTVAVPAAAESTETEEERQAKRQAEIDRLQEQLNQLSQKISSFARDMENFAQQMRQQEALGNEQDQQREGLEKAYKIKKKTFSLLDDAEGNTKELQALCNQTSANILEMSGEWEKVRKPIIEKYRSLKDRANNEADEAKSKLDRIKEMRALIRKLVEEVQQKEELFQQLQQAYKEAPKDANRSIYTLRILETVKNIKKQKVDIDKVLLDTKNLQKDINSITDTAVRTFDLVKDLLYNDAKKDPIAKQAIKSFAVIDEKFQKLFKVIDETGSFQNNVLTLESKIDYITVKTNTLNSDRIVNDLKNVKTENQNLIKTIKSKMQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q415</accession>
    <name>A0A8J4Q415_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_005120</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000196">
        <property type="protein sequence ID" value="KAF2073571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q415"/>
    <dbReference type="OrthoDB" id="10030083at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03390">
        <property type="entry name" value="PAP2_containing_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF108">
        <property type="entry name" value="PA-PHOSPHATASE RELATED-FAMILY PROTEIN DDB_G0271516-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="8">
        <location>
            <begin position="165"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="37"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="48"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2073571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="382" mass="43224" checksum="FFA0858AE41841B7" modified="2022-05-25" version="1">MTTTDNQNDKENHVSREYSNNDIKLNSNPNDNASNNKECSSSGNQCIKNNNSDNNNNNNNGKKKEFEYIPDHMHYILNFRKRHRVKFYVFDWFLCILCCFIGAALFYFVPVRGRLFRLDDPEISYPLLPELVPFSHLIVYSLVIPFFIICVVSYVLTKNISDMHHAILGLLQSFSVTLLLIAAFKCFVGGLRPNFLELCKPTPESIAAATGAGYNKIYYTKEVCTASDYDINDGMSAYPSGHAGLSATTLAFCFFYLHAKLKTFHHRGHLLIFIIVASSIVCGGLVGISRVADYRHTFLNVLAGWCIGLVTAAAMYRLNYLEIIGNRSHIPVADFWHWTWGQDDQDNQYELADYCSSNGDGTNSFDLENPKTLHESTIPLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q5F6</accession>
    <name>A0A8J4Q5F6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tetratricopeptide-like helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000001">
        <property type="protein sequence ID" value="KAF2078672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q5F6"/>
    <dbReference type="OrthoDB" id="5986190at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="260"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="391" mass="45020" checksum="277BDCE6F4B145FE" modified="2022-05-25" version="1">MQNIYKFGGIRSYCTTPFKSKVHGLVSNANNNNNNKSTESLIINSRNLLFHGRQLLERGDLDQAEKMIVHSFRNFNKKIDIHSSLFAVPSHAVGLVSLKKGKYSKGEQFFKLSLDRSETDSSYVYRNSIETSNDLGLSLLRQGKTQESFEQLQKALNLALEYQMYSLVASIYTNLGEYYKDMCYYDKSKFYHGIAHSRLMTYSSPGLDMARCFLNRSQISRISGEMDQSKEFLLEGSGILIRYLKRMGYSSNLPKHMDWAKTLIEFGHYYYKMGNIDKAKKKLNSAKKLFEEMNIKSIPDSVINTLNLAIIEKNIQLAPATSAADQQKIETHIQNLLAEIQKPYKDLQLEKFNPKLNRFNNVFDNLKNQKCQKPIPLIENLHFQLSGMINF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q6A0</accession>
    <name>A0A8J4Q6A0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003904</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000129">
        <property type="protein sequence ID" value="KAF2074801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q6A0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="591" mass="68673" checksum="A5C421A4770C98D6" modified="2022-05-25" version="1">MNQLSQFEIPKTLQKRILTILWDLIKDEEDLMDNGYCYGSSRDIIHGRFRLSLVSKYWFEQVSLLYYCFGSVHMFNSILANSIQELTSDSTKTLVPSVYEKMNSKIGNNNPYNLLSFNSRLSFYSTLNYIYINEDILDHEFDETNPLYHTQLLFQQLNGETYDHETDSETAKLYHTLAPLEIEKRKQFYKQFYSSISGFSLNTGNSLPWMDPKPLVKYLSEIANCRSHGNPLESFCLCCNFSFITYSVDKCRELKEVLLDLRITSLQIDLSMCNFKTDKHSLLLKEFISCHSNNLEDLIILLNEREPSHCLDWTVSILNLVKNKITKLSIIDYSSFKIQDNHPLFQELESFPSSSLQDINISFLTPSQTKKIFDHFGISNIFRSPPSALVSTTGKEFRINLDKITHPISYSPHIKVLSISSNETASPTIATPQDISMPKHEPFTSQQVIHALSHLYYHPLEFLFIKRAIDLDILAQFIQSNPPVNNLELNFALQGSPSFYQSISKNSRLFSIQVLIEDEKVVDLFLESIVKNSTLKYIIISFRGKFKYQTKEPLKTGDLHQSLDEKFHIYHKEYYNHIYILKERVYFGIQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q6R9</accession>
    <name>A0A8J4Q6R9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Signal transducer and activator of transcription</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_003511</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the transcription factor STAT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000110">
        <property type="protein sequence ID" value="KAF2075206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q6R9"/>
    <dbReference type="OrthoDB" id="19300at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09919">
        <property type="entry name" value="SH2_STAT_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000446">
        <property type="entry name" value="Signal transducer and activator of transcription"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000099">
        <property type="entry name" value="Signal transducer and activator of transcription"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.340">
        <property type="entry name" value="Rel homology domain (RHD), DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.240">
        <property type="entry name" value="STAT, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041604">
        <property type="entry name" value="EF-hand_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037059">
        <property type="entry name" value="RHD_DNA_bind_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001217">
        <property type="entry name" value="STAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015988">
        <property type="entry name" value="STAT_TF_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015347">
        <property type="entry name" value="STAT_TF_homologue_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041410">
        <property type="entry name" value="STATa_Ig"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11801">
        <property type="entry name" value="SIGNAL TRANSDUCER AND ACTIVATOR OF TRANSCRIPTION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09267">
        <property type="entry name" value="Dict-STAT-coil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17901">
        <property type="entry name" value="EF-hand_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18214">
        <property type="entry name" value="STATa_Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47655">
        <property type="entry name" value="STAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 9" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SH2" evidence="12">
        <location>
            <begin position="914"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="223"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="297"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="411"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="522"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="735"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="123"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="68"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="223"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="322"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="361"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="411"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="444"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="467"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="494"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="738"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU046415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2075206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1043" mass="118287" checksum="744C2945902385D1" modified="2022-05-25" version="1">MDTSKNKSTSSLPPFSFINNWENKTQLDKSNAEQQLPSSSTPSPEFLNNSQNSQNIHHILSPHSIGQNNNNNNNNNSNNNLNKSTNSNNNIITNNNNNNNNNSNTKESNSNYEKMMATTLDNIGKMEAIQKKYETEIESLMNQIQTYIERDQKQRIHSKSIEDINARLENENIQLKKELFDISRKLKEVERSDILMHSKIQSENILTNSHQYFNNNSNGNNSLSNNIYNNNNNNSNIYDNNVNGNGNSNNNNNSAKKKTSIIGGNNNGENKRKGRNNLDGDDEAALVAEALGSFGDYASKPTLKRSNSEEFSSRLPNKNHINSNSNNNGGNNNNNNNNSDWVFQEQKAKKRKESSFDEENNNNNNNNNNNNNTLNNVYNQKVPMFNQQQQIQQQVQQQLQLQLQQIQQQTLSQSPQQKQPSLSSSTNSLSSSNQNIPQPPPQLTRQNSKGSNSLKDLINVKNEKQPSNNNSNTNGNGNNNNNNNNNNLSKSGNHFPSSLSQASSIPEMETDVENDEFDFNRKEQQDDDSPDGEMSRNSSSPIDEPISDINLFKIISPGSPQTPQETLLNEIHGLQNAQREMLDKMYMAQKQFLTEPGYNEDILHTLQSEQTKLSTQIESEIQALNQMYSQTILEPNQLCKLDILLQDISIQLKQLHLFQMELNYGGNEPFPATLVITKQPFPMVISKFKQLQEDHLCVQLLTGANVDIVSYSPIKAELIFHGKALTKGNTLSGTNLGASSSSTPAPSNNPLKRHIDKDTQTLDPVKGIAKFPIKFMTGTRKSCVKLHFVLQIKTSDGSIVNVPSSTSQPFIVITNDCQWEGSEGTLLKKESFNEKFEITWPHFVNILQKHFLKATKQSPIQPTRPLSHYDFQYLNNTYFKNKTYVSHKDFDSFWSWFGKSIQTLRYKRHISTLWQNGLIFMFLKRESINQILRNQEQGTFIILFSEAFPGQLEISYVGAEKDSTKVKHYLVQSNDTSGSKRTLPDFLNECPQFTHILQLNVAMIPQTENTPIFKRESKNIVLEPYYSKRQNSQNILASGYDPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q7H0</accession>
    <name>A0A8J4Q7H0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pirin family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_002837</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="2">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pirin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000083">
        <property type="protein sequence ID" value="KAF2075851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q7H0"/>
    <dbReference type="OrthoDB" id="198735at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02247">
        <property type="entry name" value="cupin_pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02909">
        <property type="entry name" value="cupin_pirin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012093">
        <property type="entry name" value="Pirin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008778">
        <property type="entry name" value="Pirin_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003829">
        <property type="entry name" value="Pirin_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903:SF8">
        <property type="entry name" value="PIRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903">
        <property type="entry name" value="PIRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02678">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05726">
        <property type="entry name" value="Pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006232">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pirin N-terminal" evidence="4">
        <location>
            <begin position="25"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Pirin C-terminal" evidence="5">
        <location>
            <begin position="176"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR006232-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2075851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="283" mass="31263" checksum="C708CB9F83659F47" modified="2022-05-25" version="1">MSKSRLVSKIVNGMNVSDGAGVKLKRTIGGPINSLDPFLLLDEFKNDNPKDYVSGFPEHPHRGFETVTYMLEGSMEHKDNKGNKGLLKPGSVQWMTAGRGIVHSEMPKQNKGLIHGFQLWCNLGAKNKMMEPRYQDIPAKNIPQVLEEDGSKVKVIAGQYKDKTGPVEGILTNPTYLDVELTAGSKFTELIPEGHTSFVYVIQGGGRFGPIQNSKQVKTHQLAILETSQGRDSIDVLADDEGCRFLLLAAMPLNEPIARYGPFVMNTEAEIQQAIKDYQTGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q8V2</accession>
    <name>A0A8J4Q8V2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">RNA cytidine acetyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">18S rRNA cytosine acetyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_001737</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">RNA cytidine acetyltransferase with specificity toward both 18S rRNA and tRNAs. Catalyzes the formation of N(4)-acetylcytidine (ac4C) in 18S rRNA. Required for early nucleolar cleavages of precursor rRNA at sites A0, A1 and A2 during 18S rRNA synthesis. Catalyzes the formation of ac4C in serine and leucine tRNAs. Requires a tRNA-binding adapter protein for full tRNA acetyltransferase activity but not for 18S rRNA acetylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>a cytidine in 18S rRNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in 18S rRNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51424"/>
            <dbReference type="Rhea" id="RHEA-COMP:13575"/>
            <dbReference type="Rhea" id="RHEA-COMP:13576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a cytidine in tRNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in tRNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53876"/>
            <dbReference type="Rhea" id="RHEA-COMP:13670"/>
            <dbReference type="Rhea" id="RHEA-COMP:13671"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
            <location evidence="1 11">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the RNA cytidine acetyltransferase family. NAT10 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="11"/>
    <dbReference type="EMBL" id="AJWJ01000043">
        <property type="protein sequence ID" value="KAF2076961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q8V2"/>
    <dbReference type="OrthoDB" id="10067491at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990883">
        <property type="term" value="F:18S rRNA cytidine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904812">
        <property type="term" value="P:rRNA acetylation involved in maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051391">
        <property type="term" value="P:tRNA acetylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11040:FF:000002">
        <property type="entry name" value="RNA cytidine acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002218">
        <property type="entry name" value="tRNA(Met) cytidine acetyltransferase TmcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03211">
        <property type="entry name" value="RNA_acetyltr_Nat10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033688">
        <property type="entry name" value="NAT10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007807">
        <property type="entry name" value="TcmA/NAT10_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032672">
        <property type="entry name" value="TmcA/NAT10/Kre33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013562">
        <property type="entry name" value="TmcA/NAT10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027992">
        <property type="entry name" value="tRNA_bind_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10925">
        <property type="entry name" value="N-ACETYLTRANSFERASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10925:SF5">
        <property type="entry name" value="RNA CYTIDINE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13718">
        <property type="entry name" value="GNAT_acetyltr_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05127">
        <property type="entry name" value="NAT10_TcmA_helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08351">
        <property type="entry name" value="TmcA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13725">
        <property type="entry name" value="tRNA_bind_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TmcA/NAT10 N-terminal" evidence="14">
        <location>
            <begin position="8"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="TcmA/NAT10 helicase" evidence="13">
        <location>
            <begin position="279"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="15">
        <location>
            <begin position="517"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="domain" description="Possible tRNA binding" evidence="16">
        <location>
            <begin position="760"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="987"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1032"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="284"/>
            <end position="293"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="459"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="618"/>
            <end position="620"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="625"/>
            <end position="631"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="720"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF13718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF13725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2076961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1042" mass="117898" checksum="D4ED7E92B71C48DD" modified="2022-05-25" version="1">MVRKKVDSRIRTLIENGVNTNHRSFFVIVGDNGKDQIPNLHYILSKSIVKARPSVLWCYKEDLGFSKHKKKKMKAKEKSKAGGVDSINQEDPFDVFISTTNIRYSYYSESHKVLGNTFGMLVLQDFEAITPNLLARTIETVEGGGLVVLLLKTMTSLKQLYTMSMDVHSRFRTENSKSEVVCRFNERFLLSLAKCDHCLVMDDELNILPISSSSRQIEAKQAVHETPEQIELKNFKQSVKETDIAGQLIDNTKTMDQATALLTFIDAISEKTLRSTVTLTAGRGRGKSAALGLAISAAVAFGYSNIFVSSPTPENLHTLFQFVFKGFDSMEYVEHVDYELVKSTNPEFNDAIIRVNIFRSHRQTIQYIQPQDYQKLGQAELVVIDEAAAIPLPFVKNLLGPYLVFMSSTINGYEGTGRSLSLKLIKQLREQSTVVSGGNSNASISGRVLREIELNEPIRYSAKDPIERWLNELLCLDSTIIKSSPAGCPHPSDCQLYYVNRDTLFSYHKASEAFLQKMVGLFVSSHYKNSPNDLLLMSDAPDHHLFVLLGPIEESNNALPEILCAVQVSLEGEIAKESILNSIKRGYQASGDLIPWTLTQQYQDEDFPRLSGLRVVRIATHPDYQKMGYGGKALEILTKYYQGEITNLEEIESDDDQEQNKHAVSSVSQDNSTKLLDEVIKPKANIPPLLFKLTERKPEKLHYMGVSYGLTQQLFQFWSKSQYVPVYLRLTSNDITGEHTCIMLRELNNEQNNTICQTGWLSSFHEDFKKRFQNLLGYEFRNFNSSMALNILHKSKQQNTNNAIVEVSQNEMDFLFSSYDLKRLESYSNNISDYHVVVDMLPTLAKLYFNNKLKCEDISLLQSAILLALGLQHKNVDQLVVELNLASNQILSLFNQTMRKLNASLKTKKEKFVKESLALPKAAAPRTGHLGSFKGDIKETDMLPLSEDMDTELEKGSDEVKARLAKLENDSELSQYLIKGDDEQWKKALDKKGNVPSSISIKTLKRKQEDDDEDKDKKKKSNNNNNNNNKTKSGKVMKPLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQT1</accession>
    <name>A0A8J4UQT1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WASH complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_007564</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000411">
        <property type="protein sequence ID" value="KAF2071116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQT1"/>
    <dbReference type="OrthoDB" id="10261210at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071203">
        <property type="term" value="C:WASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028191">
        <property type="entry name" value="WASH-4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028283">
        <property type="entry name" value="WASH-7_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028282">
        <property type="entry name" value="WASH-7_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027307">
        <property type="entry name" value="WASH7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31409">
        <property type="entry name" value="WASH COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31409:SF0">
        <property type="entry name" value="WASH COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14745">
        <property type="entry name" value="WASH-4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14746">
        <property type="entry name" value="WASH-7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14744">
        <property type="entry name" value="WASH-7_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WASH complex subunit 4 N-terminal" evidence="2">
        <location>
            <begin position="38"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="WASH complex subunit 7 central" evidence="1">
        <location>
            <begin position="604"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="domain" description="WASH complex subunit 7 C-terminal" evidence="3">
        <location>
            <begin position="964"/>
            <end position="1132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2071116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1133" mass="131130" checksum="A01C824021155A42" modified="2022-05-25" version="1">MSSYNPASNSNNNEEYKFDAFEDGSSKLVGEQQLTKLNQFAVDYAQQIWKIEEALDDSLNEAWDINIDPVSLHMKPHEQQDLIELVKTDHKMFNKVILVFSSLCNQARILKETAESKFYHPLTIFGEVTGEASEGDVQIEVGKILPFMIDLSAFVNRCYSLIRNIVSQFASIYHSQKNIHTQFFKGVHLQAVYYSLVDLFTVLITLDSILIQNNALASSWGRYLRMVKSVKSEPGKYSVEEEEKLWHLEKLLLSIKGQLLEGFIFQECITQEFEFPGVIEVKNNKILKAEFLFNVKAMFGLLATKIMDSSELYLREKFPGFLGLYSFYLSLFKDITDKSFFKQVWEITKKVPMVAIHTNVFWFPADFISVIMPGMIKIIGNPNIMEARKEYLKVIDREFAARVKSYYLQVSRWMVRMESGSTNRGTLWDANLSKVGQIIQGILLSYHVSHLLKTMIGLHMNLTAPLKSSDVRKLFQCAEMLKAIQNTFHRRSAMISAHISMMIQHLTDIINDKLNVVRNKFAGRTNYSEVELDVIIALALTSDLLGGVATPERLTVIKLCLNVIGQVNVLKEQDIEELRIHIKRLEFLSDINNTIKHSCDCSILFWSRDLFPTYLQYLYQNPNQATSLQYTLTGLKDIVTCLEKSIHVDNPINLIDIYRNELEDMINKNIIHPLEKDIETDLRLHIHAFLHVEEKDPFKTGIKEFGKFLELKPLRFFDKTIDIKSRIAHYLDSTFYNLNTVALFDWKTYSEMRNMAFYKYGLKLLEVHLPGATLEQGLDVLEIMRNIHIFVSRYNYNLNNQIFIQRSSNSKTLNTINITHISNSIRTHGSGIMNTTINFAYRFLVQKFSVFSEFLFDDQIKSRLYKNIKYFRENKEQLGNMYPYESVSELERDIRQLGVTDQGLTFLDHFRILITHIGNAMGYIRLVRSGGLHYCSNAIKFVPDLKHIPKFEDLVQKDGLSVETFESAKNLDAVIANLSDNTSEGTEYFKMLVNVFATEFRNINNQHLRNFYAIVPALTVNFIDHMINAKDKLFKKSKAIGAETLQFTDDGFAIGVAYILKLLDQNKDFDSLHWFERIQKKCEDDQKRMLAEAASRGIKEDQNHLAVKKVQNYQMEFDLFKYSFSGARIFFKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQT9</accession>
    <name>A0A8J4UQT9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007536</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000408">
        <property type="protein sequence ID" value="KAF2071146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQT9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052050">
        <property type="entry name" value="SecEffector_AnkRepeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586:SF3">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140860">
        <property type="entry name" value="Pseudo ankyrin repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="745" mass="87551" checksum="1D2656CABD67DBDC" modified="2022-05-25" version="1">MAIFSSLKSSSSTATFSNMDYLYQSVFQHNHIRTSVFEYVKELSKNKNPMKYDDIINLDWMLSWRHYGILKEKLRDNIYLTMDPKQRALIMSIRDIQVFKLCFARYRDYFPLSYPPILNTAAYYSNLPVLEYLYKENYRSHSQALEYCAQNNNFEILLYLYNVVKQRDITPLTIIYSIRSGNLEMTRFLFDNANPSILDVSKIDRDNMFYSAASTNNLELFKYIFEKFPQTLVTRTTFKQTMKNREMFIYLFENTDYRLAFKEPGGERSPLSPIESMSLMMAQDGRFELIEYLYNSNQLPGSFFTQISHTALRYGHGEIYEYLKRQQVKEKEISLDNISIGSIKGALDNIEKVEYYHRMGAQITENCIFNACKNSKQQQVFLYVFENASLQVHESLISHTGHTYTDNLLDQCIVSNNIFALKYLISRFSHQIGTYSWRKSPLNDNSLQVLEFLFERYPLNIDDIPHISQAIYSSVTNNSLQVSMFLFQQLLSLTDEHRHYNLIIRFASTHARLDFLRYISKYYPLPLDIFYKDGRYSQEVLEFFSKHHLSCPPNALCYAMADGNIQLFLNILEAYPQLKKCVNDQSLASAISNHHEHCVEFLIENYAATITCSDLVMNMIGEVGNLSILVYIYRNIEKLSDYPNFQTTFNRAIQNGHLAMVKRLYEESSIGCTVTKDNFKTMIERKHSHLLEYFSHKEKFDKSFISPQKSPTLFLDRDSQLFNQYLQQYILNLKSKKFSLLSFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQX2</accession>
    <name>A0A8J4UQX2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007385</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000391">
        <property type="protein sequence ID" value="KAF2071296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQX2"/>
    <dbReference type="OrthoDB" id="17470at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045068">
        <property type="entry name" value="BACURD1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING ADAPTER FOR CUL3-MEDIATED RHOA DEGRADATION PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145:SF8">
        <property type="entry name" value="RE57120P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="221"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="169"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="363" mass="41787" checksum="C743E7BCECB5AE04" modified="2022-05-25" version="1">MYSINNNTTAIVENSKRKNIKQSIESVQSLFIGHFESLQQQIKDLEKRKEVLLNVTKVIDVSVIPDPITLNIGGFKHQTTKATLTKIPNSFFDLMLSGEIDIKPMTNKPNTYFIDRDGTHFNYILNYLRDEGDIQIPEDIRHCIRKEMEFYRINDYFKLDDTVTIIDQLNSLKLKLDETEEELAFKNEESKKKTKYLKEEVNSLKDKLLAQLNNLKLAFSLILNHHTFKILRDWIDNTKLTNFELLYSASKTNEYSQQAFHSACDDNGATITLVETTDGCVFGGYNSQSWNSEENEKNFVAGDSRFGPAFGNEYGYDLVIQVTQDGSYQEFPTGYNDTTGKGITTLNPTENFHIKYLEIYKCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UR78</accession>
    <name>A0A8J4UR78_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_006931</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000342">
        <property type="protein sequence ID" value="KAF2071746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UR78"/>
    <dbReference type="SMR" id="A0A8J4UR78"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051240">
        <property type="entry name" value="Mito_RNA-Proc/Resp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47933">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47933:SF11">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="158"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="234"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="460"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="30"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2071746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="565" mass="65241" checksum="49C1BD51C2947D98" modified="2022-05-25" version="1">MLKNKIISLGSIQKSNYLLSLIHSSSISYTTSTNQENSNNDNNSSNNNNNSLKPKFSSRYEIRDKLNKKAPRYDRKNDNKISFKTPATEKVKEKETAPIKKLITSVIKKEPYNNQHLMKRAPEDRSLSQKDYFEKLAQNGKFHEAKEVLKYMGEDRKSLYGYEKLLMACSKAGLFTQSWKTYNEMKKHSLKPSIYTLSHMINVCCNSLNATPSSIQERIEKIMSEIEKFDVKITVPFFNVMMKTLIRIGKYEEAIAFPNKMTSVGAKPDLATYTTWISAKAELFKSVSQYDENKRDLFNTLRFSYDSQELDEIRARGIKQIEEYLVVIQHLKKNNVLPDRRFINSLLSACRQTYHPAGVFEVWDKIQEMRFTQIINPDTKTYDILVSTANNINNIDKGIELINEIIAKTIRPDIDLINNLYRVALRASSLKKASQREKYNIQGIIDNVIEYMARFNLLPNQESFSILISAYAKLGKMNRVYELFIEMKELGLEPTIKDYNGVIKGLGSDIEKILDVFDVVVSKKLDLTPEFIQMVQTLVKRHGKGNHVERLSNGLRYLDSQKNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UTY2</accession>
    <name>A0A8J4UTY2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010322</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001037">
        <property type="protein sequence ID" value="KAF2068353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UTY2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="227" mass="26356" checksum="D55B2660522FF05E" modified="2022-05-25" version="1">MSIAEIKNLKKVETVYFFYYNNCVNTNQISITFNDGVVDRTIDYAKDPNSKLLIVAHNDAMGISSMKFTLNSGTFCSKLSIDNLFTSRKYEMELVGNQYKIIKYNPNNLEFTSWCDANVFLQIEILGSIKRHSFFMETDTWTKSLDNVPYLGDIKAIRVRAFGAQTQCHKFRIVQTCPEGQSKCLPKNTPYRPLQGYRKTLDSKLENMKIIQQYLYIPFYPYGQVQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UU59</accession>
    <name>A0A8J4UU59_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ABC-2 type transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_010173</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000956">
        <property type="protein sequence ID" value="KAF2068503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UU59"/>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="203"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="ABC-2 type transporter transmembrane" evidence="7">
        <location>
            <begin position="45"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="CDR ABC transporter" evidence="8">
        <location>
            <begin position="310"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2068503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="356" mass="41146" checksum="25F55AC10741686F" modified="2022-05-25" version="1" fragment="single">PAAWRDSPERTSITAELAEIQRTAPTIYTSDENGPAREFSTSIWYQTWEVYKRFNLIWWRDPYYTLGTLGQSIICGLIVGFTFYDVSNSSSDMNQIMFLLFEALFLGILLIFIVLPQFLIQKEYFKRDFASKFYSWFPFAIGIVLVEIPYTIISGTVFFFIIYWTAGIESDGEAGFYFWILYIMMLLFMTSFGQALGAVCVDLFIAFTVAPFLFIFLFIFCGAMIPYQSLPYFWRSWAYHLVPTRYFLEGIVTSVLKDVKIECTMEDFLKFSPPAGQTCLEYTSNFTSSYSLGSITPLSDGQCGYCAYKDGPEYYENVLSWSASNTWRNFGILIAFWIFNISLVIGFVYLTRKGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWD3</accession>
    <name>A0A8J4UWD3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Annexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_001280</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000029">
        <property type="protein sequence ID" value="KAF2077431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWD3"/>
    <dbReference type="OrthoDB" id="37886at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000002">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.10">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001464">
        <property type="entry name" value="Annexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018502">
        <property type="entry name" value="Annexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037104">
        <property type="entry name" value="Annexin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502">
        <property type="entry name" value="ANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502:SF130">
        <property type="entry name" value="ANNEXIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00191">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00196">
        <property type="entry name" value="ANNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00335">
        <property type="entry name" value="ANX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47874">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51897">
        <property type="entry name" value="ANNEXIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0041">Annexin</keyword>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0111">Calcium/phospholipid-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="168"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="179"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="210"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="233"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="246"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2077431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="449" mass="51336" checksum="3E4605AA5104A566" modified="2022-05-25" version="1">MQPQSRADMEFFRNSPYDYGKITHNIHEDIEVLKGAFKGMSADETILINILGHRNWAERAEIVQEYTKKYDKDLVKEINSYTGGKLQKTLDALMTEPSIYDTIQLHEALEKKDSSAIIEILTTRTNAQKQQIQKIYMIKFSQHLDHSISSKTKGKFKDLAIALLADREASPSQSQMDKQTQPVNQQPPQQQQIPRDSPTSSSPQRPMESPQQQQLEQPQPQLALPATQQLEPSQEQSVSSMSDNSPKQVPHDQQPQQPSPQSNVSFEKESVSPRQQQSQEHIPQQYQQQQQPPMDSQDDLVTKDAHALYAAGEQKLGTDESVFIDIFSKRSLEHLRKVEDAYYRINVKRHTLFHAIEHEFINGKLKIALTDILLYATNPASYWANLFHRSLSGFFVKNTDLIRLTVTQCSNIDSIKEAYHTRYNRSLASDITHSISGSYKKVILDIVSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWY1</accession>
    <name>A0A8J4UWY1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">thioredoxin-dependent peroxiredoxin</fullName>
            <ecNumber evidence="2">1.11.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_008441</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000520">
        <property type="protein sequence ID" value="KAF2070245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWY1"/>
    <dbReference type="OrthoDB" id="10259030at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03015">
        <property type="entry name" value="PRX_Typ2cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000003">
        <property type="entry name" value="Peroxiredoxin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050217">
        <property type="entry name" value="Peroxiredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681:SF171">
        <property type="entry name" value="PEROXIREDOXIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681">
        <property type="entry name" value="THIOREDOXIN PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 9" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="7 9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="11">
        <location>
            <begin position="13"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity" evidence="10">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2070245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="208" mass="23181" checksum="9844D698AB0FF1E0" modified="2022-05-25" version="1">MSHGHCQHKKEQIRIRKPAPAFTAQAVHNGEIKTVSLEDYKGKYVYLFFYPLDFTFVCPTEIIAFSNAAAKFREAGCELIAASVDSPFTHLAWINTPRKEGGLGGIDFPIISDLTHTISKDYGVYIEEDGHTIRGSFIIGKDGLVKHIQMNDNPVGRNVEEALRLVKGYMFTDVHGEVCPANWNADTNAKTMIPDPKGSKTYFESVNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UX29</accession>
    <name>A0A8J4UX29_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000496">
        <property type="protein sequence ID" value="KAF2070410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UX29"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="89" mass="10209" checksum="7F64C4DB8C6A4DB4" modified="2022-05-25" version="1">MSAIPDPKPDKTIISKLPNFAPSEYWELQKGSTYRMLVGELRSLKIGSEIKVNMWDTNNNYKTFQSGQYADFKSDVPGPTYIVQKYVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UX32</accession>
    <name>A0A8J4UX32_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">CYY_000550</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000010">
        <property type="protein sequence ID" value="KAF2078166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="162894at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05579">
        <property type="entry name" value="STKc_MAST_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000604">
        <property type="entry name" value="AGC protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000147">
        <property type="entry name" value="probable serine/threonine protein kinase IREH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000700">
        <property type="entry name" value="Probable serine/threonine-protein kinase DDB_G0272282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE GREATWALL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PX" evidence="17">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="15">
        <location>
            <begin position="114"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="1339"/>
            <end position="1659"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="18">
        <location>
            <begin position="1660"/>
            <end position="1720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="245"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="550"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="850"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="944"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1261"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1494"/>
            <end position="1554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1731"/>
            <end position="1832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="245"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="256"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="267"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="281"/>
            <end position="296"/>
        </location>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
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            <begin position="306"/>
            <end position="316"/>
        </location>
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            <begin position="345"/>
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        <location>
            <begin position="493"/>
            <end position="504"/>
        </location>
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            <begin position="507"/>
            <end position="519"/>
        </location>
    </feature>
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        <location>
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        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022527"/>
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            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
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            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022777"/>
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            <dbReference type="ARBA" id="ARBA00022833"/>
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            <dbReference type="ARBA" id="ARBA00022840"/>
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    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF2078166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1871" mass="207170" checksum="14797BED769E36DB" modified="2022-05-25" version="1">MKYQLSIIGDEARLDPQFNAFTVYMVNVTNQLANKEWNIYRRYSQFNELDSQVKYSDDNNNKNKSTNKELVEERKQLLQKYLTGLVKHQDIEESDLLACWLTPKNDPAFKSLANPDKCGFLIKEGHVIRSWRKRYFVLKEGIIYYFRHQSDPEPAGMIPVIGSLIKRFGETERRFAFQIIPSKKDVFPIFSIQALDEQECNEWINAIEDSQRRYSEMDLGLPDDDYKKNNGTKIKKSGSFEFSTLTSASAPTSPLQSGSSVPGINTSHHHRRRPPQKSKSDLNLSSSLSRLSIHVSGGSGASTNNNDDDGQDDYYDEGGLSYTPSLSDDMIDDDHPSHPGYSYRSMPSTPVTSGSLLNDPTGGKINSTTNSTTTKPPVSHKKSNSNSGAIPSYIGHLHHLHQPSLSSSSGSTSSSSSSGSSSSSTSSSSLFSTINQYLNPRSNSASSAGGSSSSTPTSPNLNLQQPPTSPLKSSTPSTPTSTPTPVDNDDNKSPSPIQTRNRSSPFLPLPNPNLVPLVPPSLSLDPDQTSPSLRSVMTMAAEKDKMKNTIFKNNSSNSSNNNNTPPSSSTTDSLNNSTPDNNSSKEKQQKEEEESLAKSKTTSTNKNPSKSILLSPSTTKSPSTGKKDKKTKTTSISDFIQQQHQKSSPPKSQQQPNKNNNNRNTLRSRALTLPVKPNESILHSTHQESQQSPTSNINLIREKFISKTTRFSSDGRPTFDINQKLLSTKASVDKKINNFIHTIVDAIDPDLIFHNQHYALIIGEIKKLSNKILHMSIYEQREQCTSVVHSIQTLFASAISNKELTSLVSKFLFIFSEFSRVVDVLNPIETKQQQQQQYQQQQQHNFNQLLLNSNNSGSNNNNNNNNNNVDSKTCNNSSNSNNISNNIKINPLSFSSGNILQKRLSKSLQNFNDMIPPAFSSSYQQPSFSSSFNQQSNLLTASLNSNQSTTTPTQSTTPTNTSNASSYSDPAILLSLESPNNNFRNSIGSEFDSSMRNRAISSPIIFDKNLENDRDLFIEKKSILTDLLKENYQSDQEQQRLQSAVVEIPCMSDDIRLLQVSPDVKIDSNMLDSTLTTTAETTAVESKPIPIIEKSFVCRICEETFAQSQLAKHSFYCGLINKHDFKQSSYDDRLASMISLVKGEVVHSWASPDHSIDVTMDSQIISQLEELVDYLATIPYGPSESLIQCQGIIDKIQKMIESHPTDVALITFGKRIVKIIEEKKDTYVQYANLQTAQTSQASKGKKWSMWGILPFIKSINSSSPSKDRGSSGSSSTSNSTIQQHVESSTTTTIVKQQQQSNINITSPNTSPIMSSNQVSLSSSTTTSTPKSASVSIADFEIIKPISRGAFGRVYLAKKKKTGDLYAIKVLKKLDTIRKNMVDHVIVERNILAMVQNPFIVKLFYAFQSTDKLYLVMEYLIGGDCASLLRALGCFDESMAKHYIAETVLCLEYLHTSFIVHRDLKPDNMLIDGKGHIKLTDFGLSKIGIIDDKQQGLNDQNNNNNNNSNNGNSLNSSGNNISNSNDSDKQNTSLNTSGTFSPYPPRKSTLKTPIKKPVKKVVGTPDYLSPEILLGTGHGTPVDWWALGIILYEFLTGSPPFNDDTPELIFHHILHRDRELEWPEEISADAKDLICKLLNPDPSRRLGANGANEVKKHPFFADVDWNTLVDQEMDSIFLPKPESELDTDYFWDRNSIYDDDDTDEDFLSTSTQDDNIQQQQIDIINETNILNSSDEGISNSSNKNSDGIPDKIVNNSNNNSNNNNNNISSNSNNNNNNNPNNNSNNNNNNTGIGNSIRLSAGSSSSASSNTSPTTTGINIIAPNRKGPIDNSNPLNSSSDNIEKGDPITFGNFSFTNINHLQDMNNFFLKNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UX78</accession>
    <name>A0A8J4UX78_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008537</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000535">
        <property type="protein sequence ID" value="KAF2070138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UX78"/>
    <dbReference type="OrthoDB" id="19582at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="82" mass="8684" checksum="5CBB5B990D46DB26" modified="2022-05-25" version="1">MNTLGKLVKLCNPSTGACITNAGKKSLHTNTSVFTSKGRIEARIAALSLVHKTCFNTSKCEERLSEEVGFGMGDPSLNPSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXA7</accession>
    <name>A0A8J4UXA7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SPIN90/Ldb17 leucine-rich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000258</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000005">
        <property type="protein sequence ID" value="KAF2078391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXA7"/>
    <dbReference type="OrthoDB" id="445362at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071933">
        <property type="term" value="F:Arp2/3 complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030125">
        <property type="entry name" value="SPIN90/Ldb17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018556">
        <property type="entry name" value="SPIN90/Ldb17_LRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13357:SF1">
        <property type="entry name" value="NCK-INTERACTING PROTEIN WITH SH3 DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13357">
        <property type="entry name" value="SH3 ADAPTER PROTEIN SPIN90 NCK INTERACTING PROTEIN WITH SH3 DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09431">
        <property type="entry name" value="SPIN90_LRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SPIN90/Ldb17 leucine-rich" evidence="1">
        <location>
            <begin position="158"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="341" mass="40143" checksum="BDFFF814B4D3EAFB" modified="2022-05-25" version="1">MSDEYQHQEVEEQEQQINVQDIKDSIYGIVDKEHIDVELIRDLIQKIQQLEINDAIDSIKHENYHIIRLMCREAGRSIDTQLVTEIVQFINGVSNEIKEIINIIIEEQGFKWIIPNLFMVDERTTALYYLDLINFILTQDEYSDSNSFQNEFNRLDSIFLESLIDLALVYNGYYDTAPLYKCFYTMFGYQKNTICDNYSPLVRKLYSIQKAPEFGPELIALLNRDIEYKLLPQCLSLINDLFSFSQEFNIGCFFYTLDIKVIIDIIIREIHNLDEMDPARWQYLEVLSNIIDHSEYQKLEYKVQDIRSVVSDLLDERSTEKDPISGTLAQTILNKLQNFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYW2</accession>
    <name>A0A8J4UYW2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Synaptobrevin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_006563</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Endomembrane system</location>
            <topology evidence="9">Lipid-anchor</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptobrevin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000309">
        <property type="protein sequence ID" value="KAF2072118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYW2"/>
    <dbReference type="OrthoDB" id="27923at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15843">
        <property type="entry name" value="R-SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45806">
        <property type="entry name" value="SYNAPTOBREVIN HOMOLOG YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45806:SF1">
        <property type="entry name" value="SYNAPTOBREVIN HOMOLOG YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0564">Palmitate</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Longin" evidence="11">
        <location>
            <begin position="8"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="12">
        <location>
            <begin position="127"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2072118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="21498" checksum="602C293A22BE2342" modified="2022-05-25" version="1">MKLVALCLAKRNGDTIDSVSNLSDFGFFERGTIKEVIVASSKIFINQFNINTKHSVPYEDYICYIDIRNDFGGVVYTDKEYPARVAMKCLTEMINEYIKKTQGIIGQPFPEMQHILNKYLDPKGQDKILEVQNQVNEIIPIMHKNIEEALGNTAKMETLLEKSNDLSGRSKLFLKQAKKANRRCCIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZ61</accession>
    <name>A0A8J4UZ61_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_004557</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNase II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000164">
        <property type="protein sequence ID" value="KAF2074115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZ61"/>
    <dbReference type="OrthoDB" id="10261598at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004531">
        <property type="term" value="F:deoxyribonuclease II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004947">
        <property type="entry name" value="DNase_II"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03265">
        <property type="entry name" value="DNase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035280069" evidence="3">
        <location>
            <begin position="27"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2074115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="671" mass="76894" checksum="E64A4F6AFE30F482" modified="2022-05-25" version="1">MKEIQRRQVYVLVYLFLMVCIGNVRGENEMCRADKILTGKDDEVDWWFITKVKGFGDVYLYVDSNMDNTATFKTGYYLRSVYSALGSTLSPFTGEGVNNKKNFISFCDHAAFNQPSSDPKKEPVFNKLYVEGAHQKGIIGWTEVSDTQWKGFYIDHTLPYFPQIHSKTFMNSPINFDPEKITTPRTEMKLYLTSMYFSYYGWRKPFFGRNLMPYDSNGGCVKDYLLKHDQDNSAPPIKLREHFINPHEKETKENERITHNERTGSGAFLKMDDIFPFAIIHTHKQKPSQHIFCISIDTKDKFLDFFKHYQYISNEGIVSNIHKVSDSSLSNIKSYISLYDEKGEHKQPTKVARTTGFKNQKLSFVKGITAESTINVNLGPAIGNDVWKGLIANQASKAEPYLENEAKIFISTWSDGDKEVWWRKNNQIKEPVFIADIYNSKIKWKSNTHDEHSKVAFTESQDHKLWNVCISGGNLYGHSKTDPIKSSIILCLKLDALHSALTKLKVRTWDGTTSLAEETEYDAYQKAFGDLKKSVMKMDEDKYVEFIGSNNVDITKAPGYKDPLASELTISIIKSKRLCKKLLDQSSETPSFKYSQPNDQGLINQVATANLHTIKFENSYTVKKPKIAGRHQDPDGDRLVQLGAKEETFMDLFNTLIPPDASKEYQLQLYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZJ8</accession>
    <name>A0A8J4UZJ8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Twinfilin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_004147</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with G-actin; ADP-actin form.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000141">
        <property type="protein sequence ID" value="KAF2074565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZJ8"/>
    <dbReference type="OrthoDB" id="10006997at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003785">
        <property type="term" value="F:actin monomer binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11284">
        <property type="entry name" value="ADF_Twf-C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11285">
        <property type="entry name" value="ADF_Twf-N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000042">
        <property type="entry name" value="Actin depolymerizing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000007">
        <property type="entry name" value="Twinfilin-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028458">
        <property type="entry name" value="Twinfilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13759">
        <property type="entry name" value="TWINFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13759:SF1">
        <property type="entry name" value="TWINFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ADF-H" evidence="12">
        <location>
            <begin position="6"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="ADF-H" evidence="12">
        <location>
            <begin position="175"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="305"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="311"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00056419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2074565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="340" mass="38093" checksum="D7665FDAA70F962C" modified="2022-05-25" version="1">MSHSSGIPVSQELISTFGSSNQDNTRFIKVVISEDQLVSSHVESISSDFESDMDKIESVLDSSPCYILFKKDDKSIEVTGCNWIFMFFVPDNAKVREKMTYAATRSTLKRELGIAHFVDEIYSSNKKDFSKKGYKQHLIHQESEAPLTMEEQQKNDEREQGVFVGGGGASMHAHGVAFPVEPKAQSAVQDFINDKCNYISLGINIQDEKIIFSSQENTDINSISKHISTEEPRFHFFRYSHEFEGENLNSIVYIFSCPDGSAGTKSAPVKMRMLYSSSKANVESLVSKSLKIDLKLEINAPSEISAQSIETELHPPKPEEKKAFTRPTRPGAGSRRLVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZL0</accession>
    <name>A0A8J4UZL0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF5009 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004080</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000138">
        <property type="protein sequence ID" value="KAF2074610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZL0"/>
    <dbReference type="OrthoDB" id="2149840at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31061">
        <property type="entry name" value="LD22376P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31061:SF24">
        <property type="entry name" value="LD22376P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035243817" description="DUF5009 domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="334"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="403"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="490"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="522"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="553"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="587"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="620"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="649" mass="74080" checksum="A5C6FAE3408483F8" modified="2022-05-25" version="1">MMLVRNIFYLGLIWILFITIVNASSSLQHSSSHSSWIINSESENNSNSSNLPDINTLSYDQAYIKVISNVASALNCSQKLQIDEIELELWYVFDACYKCKSQLLLSTVTSESDYVAVSTTFGYRFNATIKSKTGQKNKLFLEFNQKFGEYGVYHIHINLTQTSGEARQLIEKIEIISKPYFSNIPIAVMFAILITLSIIWPIIIYFYKRSKRDSLEFQLQNLVNDGLKKDRMKSLDVFRGFCITIMIFVNYGGGGYWFFNHSYWNGLTVADLVFPWFIFIMGIAMPLSFNALDKRGTPKRLIVFKLVKRSVILFSLGLFLNNGQHLTHWRILGVLQRFAVSYLVTGLIMLFIPLWRPRVPDESIEEVQVMIGSPHSSSNNIQLVNSHDNQLLKLFEKNKFIDIFPYWIQWAVALAIFAAWFLIMFLLPVPGCPTGYLGAGGLSDDGRYPNCTGGAALYVDRKIFTLAHIFQTPTCQPIYNTGAFDPEGTVGYLTSIFICWLGVQAGRTILTYKSNRSRLIRWLLWSTLLCGIAAGLCQVSQNDGWIPINKNLWSPSFILLMAGFGYLVLFIMYIIVDIKKLWNGSPFIYVGMNPISIYMGHEILGGYFPFSFYMSQPTHTLYLLSNCIGVACWLLIAYQMYRNKLFINI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZS0</accession>
    <name>A0A8J4UZS0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1</fullName>
            <ecNumber evidence="5">3.1.7.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Metazoan SpoT homolog 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Penta-phosphate guanosine-3'-pyrophosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_003921</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">ppGpp hydrolyzing enzyme involved in starvation response.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>guanosine 3',5'-bis(diphosphate) + H2O = GDP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14253"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:77828"/>
            <dbReference type="EC" id="3.1.7.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MESH1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.7.2" evidence="5"/>
    <dbReference type="EMBL" id="AJWJ01000130">
        <property type="protein sequence ID" value="KAF2074775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZS0"/>
    <dbReference type="OrthoDB" id="430679at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008893">
        <property type="term" value="F:guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3210.10:FF:000012">
        <property type="entry name" value="HD domain containing 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052194">
        <property type="entry name" value="MESH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46246">
        <property type="entry name" value="GUANOSINE-3',5'-BIS(DIPHOSPHATE) 3'-PYROPHOSPHOHYDROLASE MESH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46246:SF1">
        <property type="entry name" value="GUANOSINE-3',5'-BIS(DIPHOSPHATE) 3'-PYROPHOSPHOHYDROLASE MESH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13328">
        <property type="entry name" value="HD_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="12">
        <location>
            <begin position="36"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2074775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="211" mass="23952" checksum="1057715D4E1A6D9C" modified="2022-05-25" version="1">MTSQENTDIATLMKTIEFASIKHRDQRRKDKHSTPYINHPIGVANQISQIGKVYDTVVLQAALLHDTVEDTNTTKDELVKEFGQKVADIVMDVTDDKSLPKVERKKHQVEHAAHISREAKLVKLSDKLYNLSDILGNAPPFWTVSVIQGYFVWGKAVVNQIRGTNKALEEKLDQVFASSFIYKDGATYRAIPCTPEEEPQFLESYYKEIAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0M1</accession>
    <name>A0A8J4V0M1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MACPF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009272</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000668">
        <property type="protein sequence ID" value="KAF2069407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="21110at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MACPF" evidence="1">
        <location>
            <begin position="1358"/>
            <end position="1669"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1669" mass="188125" checksum="AC8B7AAA8BC9DD55" modified="2022-05-25" version="1">MELFIGGTRVVIDEKDYWICGYNPKVSKATFQDSALSFSMHMPTLVGPIDNFVCNYKMAPNIINYCVLGKKYDGFDDIIQVSTKLMSDTEISFLDQTVLEFYIGSPLYPNKEKRIIVENPFYLPFDPQEELTNYKSYFSDANSFRPHSHMAVSFDVQVEKYSFFLLYYQNVNSNFNFFPIRGSLYSKELMSLIPVTFFNSQGPKNIDFKVVTRSGDSNVAVLEKQETMTLVEGIYFNILSLNQLDNLILAKLLISFTFVDDNYSFEYNFNGISKTEQIQFPFGYQLKTNMLIVPLLITPTPNNMISLKLKSIFQGEYINPFSFSSSNMMIDVNPPIVSIKSVGSFGVFQNIKSIELNILDTDSAVYEIKIGSYIFRAVDCLVFGTLNNGDFEITLDLTKQFITSVTPTVIVSDIYGNSVSVVIENFSESRVSSFDDIVAFEFLHGSIDTSIINRNTQAFLKIKNMDPYFKPVFTFFDRPFKSDPNEKFSFVATYQQDIDMYQFNIQLPNRPNIDSLEFSLYIAPFNISNYDLQSKFGDSAILKFHLDEIDVYPPMVTEMEIISDNEFDDILGWYLTISDTPTGLYNGSVKVISNYDGEPRVFVLSKENRVSGNEFVGKYKIAFEIQNQIQQTFFISEVILFDMNGRVSKYPPELHLLSPFIEISSSDFKVKNQIPYLPPQGFLEETISPILVSFTTDHGEKNLLTIKFQTKDAESGISTRHNPYIYIKGFMGHVQIIKSNIASIDEDGKSVSYKATATLDYELSQYGCYYFIYGITDNHLNTIGMDVTTLQVANFDYYYKNPILKMEPIIESYSRISQLGGYLTIYGNRFVYEATKETTSTMVMIDNRAFTPEFISPSLIVLNIPSSFVYSYLTGRVTSFDLSIQNSFGTSNIITISPKSFSRYLSMYVDPESTCASGCGSFHSPYSSIKSALSNATEHSTIILKDGMYRGSDNVDIEITSLSNIIIRSINGFLKTKIDCESYSLGFSIIGVQAIGITGITFMNCVSNKGGAFYIQDSSVLLTSVQFLNNLATNGGAVYSSRSDITILNSVFYGNKVINNGAAIYSDLSSIDIKGDFTRFSRNTNRNDKAIFNGSENNNNRDILCKNSTITVDSVVSLKNAQFSCVDGCDYTYKQESLCSDGEAPKDESLHCISGDSCLINSKCPCYFSGMVLEEYEPGCRLASEFACQVKERLPLPEIKLDNFMGGMTKFIVKIYGFVSIKESKYVDFTFYGSHFGFLFKLDRATKLSFSENSKFNETISIPLSDKQSHYIEILLFSNHPNGGDRLFNLAPFKDSSTTLFYSNQMCNDGIRNQGEKDSCDSNLPIFNPNSIPSCGDKICNETPNSCFQDCFDLVNLKKSQTTIVSKKLPGTDQLSFGLNVFSVEESQVPIFDFDYSSSETLKHPLTGIKYHIPKDFINFAIYPQCTYSPRVSTFLTLANLKNNLYSEAFSGETPTTDEAKKYFSLEKTVVEYSRIRKTPQQAIVRTDLYCKTSFIELDLDNIHFHANFLKQLDSVKVVEDMVNVLKKYGNHVYKSTYLGGKVSQMALAEPYAINVDLKDRAAKIFSNSIVTPVFSMTTPNDVSLATSITMSRLFNYGGKSASFSPSTDSKSSPLYQEWSTSLNTLPAAIQYTLYPIQKLLDKKGFINSNGVDIAAMWAQANTFYVNSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1U9</accession>
    <name>A0A8J4V1U9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">isocitrate lyase</fullName>
            <ecNumber evidence="2">4.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_002891</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Can also use Mn(2+) ion.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.1" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000085">
        <property type="protein sequence ID" value="KAF2075808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1U9"/>
    <dbReference type="OrthoDB" id="4078635at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004451">
        <property type="term" value="F:isocitrate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00377">
        <property type="entry name" value="ICL_PEPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000005">
        <property type="entry name" value="Isocitrate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039556">
        <property type="entry name" value="ICL/PEPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006254">
        <property type="entry name" value="Isocitrate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018523">
        <property type="entry name" value="Isocitrate_lyase_ph_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01346">
        <property type="entry name" value="isocit_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011645">
        <property type="entry name" value="PRK15063.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21631:SF3">
        <property type="entry name" value="BIFUNCTIONAL GLYOXYLATE CYCLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21631">
        <property type="entry name" value="ISOCITRATE LYASE/MALATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00463">
        <property type="entry name" value="ICL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001362">
        <property type="entry name" value="Isocit_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00161">
        <property type="entry name" value="ISOCITRATE_LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="436"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="106"/>
            <end position="108"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="207"/>
            <end position="208"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="328"/>
            <end position="332"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2075808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="468" mass="51940" checksum="CDEAD3834A10440D" modified="2022-05-25" version="1">MNISEDYNQLKHKINVKDYIKEEAKVLQKDWDTNPRWAGIKRDYTAEDVIRLRGSFKEEHTLAKKGAERLWELVNQKSSWVPALGALSGNQAVQMVRGGLKAIYLSGWQVAADNNTSGQMYPDQSLYAVNSVPELIRRINNALRRRDQIEFSEGNLEHQYLPTPIVADAEAGFGGPLNAYELMKSMIEAGASGVHWEDQLASEKKCGHMGGKVLVPTCQHIRTLTAARLAADVMGVSTIIVARTDSLGASLITSDIDARDKKHITGDRTVEGYYNVNKGIQVAIDRALCYAPYSDLLWCETSTPSLELAKQFADAVHAKFPGKLLAYNCSPSFNWKKNLSEDQIASFQRGLSEMGYKFQFITLAGFHALNLTMFELAKEYVKDGMTAYVNLQEKEFALESEGYTASKHQREVGTGYFDTVSQIISGGKSSTLALVGSTEEEQFEDQPTKKKKTTHATSRSSKAPVPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V228</accession>
    <name>A0A8J4V228_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002647</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000075">
        <property type="protein sequence ID" value="KAF2076033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V228"/>
    <dbReference type="OrthoDB" id="21053at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="270"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="436"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="611"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="667"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="163"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="445"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="477"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="497"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="612"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="630"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="671"/>
            <end position="688"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="725" mass="79495" checksum="F2A4232BC0D3F5A9" modified="2022-05-25" version="1">MVHRINMILNDDDNNNNNNNVNNNNNNNIDIQELKTFGKIAINNSAPSTPIAANRPRQPFLNEEDEEELEIVTDDHPTSPKKKFRASDSQVEIPKISFTTGISSEEASLQELARRLNNLNNNSPSISSSSPITTSTANTLSKSVSAMNLRSYSLNTSSSSSINNTSLANINNLTCDKKELEQQVVDSDDEYFDDDEEEDELDEEEQEYFRSKFDEMRNSAIHAANVHASNNINNNNGPVNSHVHNASGSCCNTSTKLGIFNYQQHHYGNNNNLQHVPSTTTTPLTSPKSNPGTPSRPFPTLDYKIKSESVKQNPDGTYSCPYPTCNKTIKGNKGNLSSHLRWHRRLDAEAGELRDTNPMDEGEEEFTTPLKPSQRGVQLRNQMIRYGLNLFRQDSQGKYLCFFEGCSLRMLTNFSRHIAKHERKGDRIKPDLVPLIPAYNGNNNPGSPSIKRSVSSPSSPTTTSTPFSPILSHTHHNSASTTPCQSPLTGSTLYNNSPSIDPSSPSCSSKLKRKLTVNTKIISTATSTSNSTTNTPSLYSYSEPCTPTMHRINLFPATSPRSLSSPSFTSLTPPAPTANLNETTPLIVGGGSKGKLDSIIDCVFNDSEFSAVPSSPSGSPMRNSTNKCNLPLSPLSPPTSSSQPSGKFGYPQKALFKDIQWDYSRSPTKKSTTPLLMPTSPLSQSSSSIVPFSTLYSPAPLIKQRSKEDEVVAKSLLNLSHGDSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V238</accession>
    <name>A0A8J4V238_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pesticidal crystal protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000842</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000017">
        <property type="protein sequence ID" value="KAF2077880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V238"/>
    <dbReference type="OrthoDB" id="23795at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.10">
        <property type="entry name" value="Pesticidal crystal protein, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036716">
        <property type="entry name" value="Pest_crys_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35598">
        <property type="entry name" value="ENDOTOXIN_N DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35598:SF2">
        <property type="entry name" value="PESTICIDAL CRYSTAL PROTEIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56849">
        <property type="entry name" value="delta-Endotoxin (insectocide), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="455" mass="52912" checksum="ADDEE2D8B221187E" modified="2022-05-25" version="1">MSQPDLTVIEKFCPLAAVGRAVLGDEYKLDSDDYMEIIKGTIDCIPIVGSLVNSVFSIFWKKAFPVEDPYLRKADFEKRMKKLISDMENKMDKKIDDEIVKFCESKFQSLCSAGFGFAEIEYIYDKNKKEGKPTPDSVKRYMAANHVSFRNLLLECLIFFSREDKIDLLCKLYLQTVVMYIAFLRDTHFYGIDWGLPEEYVIGDEKIKSMATKTHETIELAHRYYSKFFKKLNPPRKNRDEDEEEEELKCDYRPLLAQMTYSDLTRYNFNQIWLQPTTKEGKVWDNDDDDEGYEYRLPLSKRIYIADFAKMDVWYGIFIPIEYGENSVNSVTGTIGMGSYESNGLMFKCFYFTKPKTLTFRVLANIDSKAQIEIGIREIPEGKYPKDPEASREVATKSHSEIINYKKLFESSSCTIAEFVSKQYKFEKPGSVYFEAKVLGEGDTRNLYSLEIIDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2F6</accession>
    <name>A0A8J4V2F6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ELMO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000008">
        <property type="protein sequence ID" value="KAF2078270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2F6"/>
    <dbReference type="OrthoDB" id="67155at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006816">
        <property type="entry name" value="ELMO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050868">
        <property type="entry name" value="ELMO_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12771">
        <property type="entry name" value="ENGULFMENT AND CELL MOTILITY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12771:SF51">
        <property type="entry name" value="LD01482P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04727">
        <property type="entry name" value="ELMO_CED12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51335">
        <property type="entry name" value="ELMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ELMO" evidence="1">
        <location>
            <begin position="124"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="291" mass="33984" checksum="90E75E3C7B114628" modified="2022-05-25" version="1">MECPLIIWRLYKFIVHLITGKSEIERICYNDELRSLERTKLIEKSINNSKQLVEIKRLIQSEFDAEEISTRIVDIKKMNKSLLAFDSKVLPNLIAALEPITLFIALKQTIKQDVSILYDKENKEHEAKLEELWDALKPNIRRSGRLTSEWGDIGFQGKDPATDFRGMGILGLDNLHQFSIRHSQDATDVLRNSTSKCVYPFAITGINITALLVRLMETDHFKDYFYKHGSTREQFHFVYAQLYKAFDEFYQTKNPVNIMSFGAIMKEFQEKVSVHFHFQPAMNNQTMNYNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3G0</accession>
    <name>A0A8J4V3G0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">rRNA methyltransferase 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_001910</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000049">
        <property type="protein sequence ID" value="KAF2076783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3G0"/>
    <dbReference type="OrthoDB" id="270651at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016435">
        <property type="term" value="F:rRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18105">
        <property type="entry name" value="SpoU-like_MRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047182">
        <property type="entry name" value="MRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047261">
        <property type="entry name" value="MRM1_MeTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004441">
        <property type="entry name" value="rRNA_MeTrfase_TrmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013123">
        <property type="entry name" value="SpoU_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00186">
        <property type="entry name" value="rRNA_methyl_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46103">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46103:SF1">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08032">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="RNA 2-O ribose methyltransferase substrate binding" evidence="12">
        <location>
            <begin position="192"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA/rRNA methyltransferase SpoU type" evidence="11">
        <location>
            <begin position="256"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="43"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="84"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2076783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="412" mass="46629" checksum="FF0BC4AE0D136310" modified="2022-05-25" version="1">MFRYQLNKINVKSIQRCFSTSNRSSSTAKKSLFSDNDDFIGDSGHGHHGHSNRNQFDRKKRNNKFNNNNNNDNDDFMMDGYKRNSNSNSKHNNSSGSRYKNNNEAKFIQEAMKKKYTLVGEGLYGIHPTSIAINSENRSFNALYIQNSLLSKLENISDVNTSSIVRDEDYFKTEDSLNDLDKSFDRYNDKKNIEKIESVLKKAIEFNIPIYSVKKTVLDQFSKDRVHQGIVLDVSPYTLLTIDSLPSQSNKQSPPLWIFLDELWDPKNIGAIVRSCHFFKVDGIVVTNTKSCPITPESSKNSAGAVEEYPIHRAESVIGFIRASKQNGWRIIGTSPDDHENIKSHNLNQFKLDQPTLLLIGNEGFGLKTKMIQECDSLVKIVGGTENVDSLNASASTAVMIYQFINTRPKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3K8</accession>
    <name>A0A8J4V3K8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001806</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000045">
        <property type="protein sequence ID" value="KAF2076903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3K8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035194672" evidence="2">
        <location>
            <begin position="20"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="107"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="173"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="251"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="409" mass="40862" checksum="8796B5CEE93A38D7" modified="2022-05-25" version="1">MKVIISTLLIILLFSLVNANDSTFKSGKIERNHSTTGPISTPTPDNSSVPVNITNSSAAPVNVTNSPSAPINVTNTPSAPVNVTNTPSAPVNVTNTPFASPSNQTVNSTSAPLANSSSSHETSVSSTQNSSTASTRVPITIQNDETPSPVGSKFTNDETISEKASSNENKTMVSTTPNPSSTPSSSSVPVESHTPSPSATSPAIVSQAKTPKPNHLAKKEFPKVSGSPKPVNPTPSNSPFGTPKPNNPTQSSSPSSSPSVSPSSSPFGSPSSSPSSSPSVSPSGSPSSSPSSSPSVSPSSSPSSSPSSSPSGSPSSSPSASPSSSPSSSPSSSPSSSPSSSPSSSPSSSPSSSPSSSPSSSSTSPSSSPVGTTMPRHLLEHHDKHVSNKARYSYVSNNGFFDQSGEANA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V437</accession>
    <name>A0A8J4V437_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Gelsolin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008293</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000498">
        <property type="protein sequence ID" value="KAF2070394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V437"/>
    <dbReference type="OrthoDB" id="6375767at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051014">
        <property type="term" value="P:actin filament severing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008154">
        <property type="term" value="P:actin polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11288">
        <property type="entry name" value="gelsolin_S5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007122">
        <property type="entry name" value="Villin/Gelsolin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977:SF129">
        <property type="entry name" value="GELSOLIN-RELATED PROTEIN OF 125 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977">
        <property type="entry name" value="VILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00597">
        <property type="entry name" value="GELSOLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00262">
        <property type="entry name" value="GEL"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gelsolin-like" evidence="2">
        <location>
            <begin position="247"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="2">
        <location>
            <begin position="604"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="708"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="866"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="64"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="708"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="783"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="822"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="870"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="896"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="909"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="920"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="932"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="956"/>
            <end position="967"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="977" mass="110490" checksum="6E34C950FC12008C" modified="2022-05-25" version="1">MDTATLDDDSIIVEQDHIKEDLVADGAVEITKSVDVDVNNSNSNDGDADKQVDQEQEKEKQEEQQCNTTEATAAPVTTTTTEATAPTTTTATEPAAVIQSSVVLHHSAKLIQVAGWEEPFCYISVPLSVESLNLEDVFIMQSDAYMFVWCSEQVASQKKAKAIQMAQKLKVEIGCERSVQVLEFGSEHPTFTYCLGLPKGNKLVVEKKENDIFQKDEDDGNLLPEVFLYQLSYADGKPTVTPIDESIKQEMLVSDSCFILDCEHEIYVWQGKKAKKQTVDTMIPLAKKVWSQFDRPYYFKENTQPVITVVFEDCESCLFKSKFGTWKEKAVQSYLSLGKKKEQLVFEVESMHVDKEITQIHLGTDNSKGKLLVWSCLSNGKWNKVEEEDFGIFYSNKSYVCHYIYQPEGKSSIRSVVFYWEGMYTNQRNYISYKFGLYKEIQKKMQSLQSDDPIEYRISQHKEPQEFISLFGTELLILNTELSMRPMMFQVRNNLGVELIEVSASRLCSLDSFVFIFPGKCILVWYGKASKQDERDLALDLYTFLPPEYEAGIKEIEEGQETDAFWKLIGGKEDYPQHLHMEGKPSKLKLFVTSENSGIFKADQVVPFSQIDLNPQENVILDVFDKVYVWKGSKTTDSKFDSTVDVANQYIESTPESDKRPQECTVEIVNDGSETPLFKSYFLSWKNSTKKEFVDPLSLKPNPFLKPKVETPKVDETPKVEETPKAEEPKIEQTKVEETKVEETQVKEPKVEETPKVEETQVEEPKVEESKVQQTKVEEEETGSEKQINKESIQEEIVDQANESIVNVEQQEQEEEEKEKVTTPNINITPPSTSTTSTPLSLSTPALPKPTFVSGNEMLFAYEFNAPIHSPRHGPASKKKKNRRNRNLSTGAIHMSPSPKSSVLSLPKSLKDIKHLNLDDATTTTHSSSPLSQSTPPIESSSPSSSTSQTTPPPSRKSKKNNKKQHNRSSSLTTPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4I1</accession>
    <name>A0A8J4V4I1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Aquaporin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_005111</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000196">
        <property type="protein sequence ID" value="KAF2073562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4I1"/>
    <dbReference type="OrthoDB" id="13694at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000062">
        <property type="entry name" value="Aquaporin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139">
        <property type="entry name" value="AQUAPORIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139:SF199">
        <property type="entry name" value="MIP17260P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="110"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="190"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2073562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="30214" checksum="02E033D47D7B32ED" modified="2022-05-25" version="1">MGKFKVIRFFTYDPTKDPTKMVYRKPISKPRKAFKGFVSEFIGTLYLVYFCCGSVTSAFAFPSGDFAAKILLACIIQGFALACLIWAVSGVSGCNLNPAVTLANLLSGRVGLINSLSYIAAQVLGCIAGAALLYACLPDLYRGDIGVTHLGPGMNTGEAFLLEMMVTCLLCLTVLGTSVFNVWDRRMNRVAPYAIGLALLVGVGIGFPFTGGALNPVRVLGPSIISGVWSNHWVYWLGPLVGAMIAALIYRCLLQERFDVIHKPGYVEPLIDPTTAVAYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4T2</accession>
    <name>A0A8J4V4T2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004801</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000177">
        <property type="protein sequence ID" value="KAF2073877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4T2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="270" mass="31408" checksum="BD6E25221ABA9722" modified="2022-05-25" version="1">MNVLTRDLQDKTIEGFILMKKKFIKKRVFFSYLLQYPILIKVQSDNFNYEKWNNCKNGTFVLDVDQLQDTIQISFFKYLLFYHDLKILNYDLLQDTLIDQIGNIKIVEHIDTPQSISNTPSHTTNTITPTLGLVRDSNSNNNSTNTKHPLPLPSLCSFSKCLNSQIYIYDKSLSKEILVSAFCNSNQGDLFIDLAHLSVLQSIVFRNCESLLIRNLYLFPSINEKELEIQFNDLEKEIAHTLKKLLCIKAQITYIKDNYIVHKNNNIKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V589</accession>
    <name>A0A8J4V589_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable succinyl-diaminopimelate desuccinylase</fullName>
            <ecNumber evidence="5">3.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_007029</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N-succinyl-(2S,6S)-2,6-diaminopimelate + H2O = (2S,6S)-2,6-diaminopimelate + succinate</text>
            <dbReference type="Rhea" id="RHEA:22608"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57609"/>
            <dbReference type="ChEBI" id="CHEBI:58087"/>
            <dbReference type="EC" id="3.5.1.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route): step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M20A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.18" evidence="5"/>
    <dbReference type="EMBL" id="AJWJ01000353">
        <property type="protein sequence ID" value="KAF2071654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V589"/>
    <dbReference type="OrthoDB" id="10059875at2759"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00021"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009014">
        <property type="term" value="F:succinyl-diaminopimelate desuccinylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03895">
        <property type="entry name" value="M20_ArgE_DapE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010182">
        <property type="entry name" value="ArgE/DapE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050072">
        <property type="entry name" value="Peptidase_M20A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033687">
        <property type="entry name" value="YodQ-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01910">
        <property type="entry name" value="DapE-ArgE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005306">
        <property type="entry name" value="PRK06837.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808">
        <property type="entry name" value="ACETYLORNITHINE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808:SF25">
        <property type="entry name" value="PEPTIDASE M20 DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0170">Cobalt</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="12">
        <location>
            <begin position="216"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2071654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="435" mass="48836" checksum="52C73A46CE542E21" modified="2022-05-25" version="1">MSDIDKVSFPTKQEIIDSVDRLQNEAIEKLKQMVSFDSLLGKENDIQIYMEEVFKSLDLKVDRFPIIKEDIEKLPGFSPVNWSYDGKENVVGLYEPSPSSHCSLDAEKRKSLLFNGHVDVVPTGREGLWTQHPFSPYVKDGRLYGRGSGDMKAGIVAFIIAFKALRELGFVPASKLVMQTVVEEECTGNGTLACLAKGYNADAVIIPEPFFNIVTAQVGLVWCKVTVRGRPAHTLEMETGINAIDGAMYVVGELRKLEEEWNKKEICHESFKQFPHPLNFNLGIINGGEWTSSVPCECAFNLRAGFFPGTPLEQVREKVIQVIEKACKEKSLPYNIEWNGFQAEGCVHPQEGQMMKQLGDTFRNTLNKEPVYSPLLCTTDTRFFELYYNTPATCFGPEAKNIHGIDESVSLDSLRDVTRVLACFISDWCGLQPLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5F5</accession>
    <name>A0A8J4V5F5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein L7Ae/L30e/S12e/Gadd45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000574</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000011">
        <property type="protein sequence ID" value="KAF2078103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5F5"/>
    <dbReference type="OrthoDB" id="20109at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042848">
        <property type="entry name" value="Rpp38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46948">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46948:SF1">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="89"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="337" mass="38816" checksum="6C9BC5643520C1EC" modified="2022-05-25" version="1">MSTTNTNTPNKNVQKKQQAVVKKKKYLVFKDTIASPYLNQPLSCISKEYSENILNLLKQLLSHDVEKINDNRMKKKLVFQSLKTEFKLIKKCNNRINVTKKKIQTLKDNIKKEKAATDNSSNTKKRKDQDDIEQYVLGKLKNNQDYDKLTATLNELDIEHKKIMDKIKDLKNTDIEPIKQQQTSDSESRMNQFWIGVNKITRELEKTPVHTPPNIRLIIVCPNQEIPILTSHLPIMAYMRRIPICLISKDTEMIFSNSIGLPNTGLALAIKETKPDEPTDEALEDFVRVSIEASKSVNSLDFPWLPQKYTSAHLTEPIPIKYVSTKVIKEGNKRIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5M8</accession>
    <name>A0A8J4V5M8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003923</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000130">
        <property type="protein sequence ID" value="KAF2074777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5M8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="204"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="380" mass="44590" checksum="DA5F81CB95E90178" modified="2022-05-25" version="1">MIVYIRTRQSYQYGRDRTRCPVHPGDNIMVLGNYVNVTDKRDAPFNWYFNGKQLNANKSFQHYNIKDKDVIETNLNPLALAVLYITIKRFEDQLAPGVDKHALIQEKEAVKVLYLGEVPYLGEQVYRFAEYTKYKLHIDEINNVLEEYRLFDKKKEDPLGHFISRVHEKFNPDKNDYFRRELQELKERRIKAIRNRFITPSILSYSSSNNNNNSNNKNNSPQLSDDKTLNHQNTINLATINISTPKINSSNNLLDRFNNTMDQIEVDKSMAVICNNNSNIININDDQQDYNKKYSTTEETASDTASISISDTEEYDSDNADDQEMSFDYEVTLNKSKIKSNEKTLYERLQFIEQENLKLLLNYPKENLNLLPRLFHFEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V607</accession>
    <name>A0A8J4V607_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">protein-histidine N-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.85</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_000014</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-histidyl-[protein] + S-adenosyl-L-methionine = N(tele)-methyl-L-histidyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19369"/>
            <dbReference type="Rhea" id="RHEA-COMP:9745"/>
            <dbReference type="Rhea" id="RHEA-COMP:11600"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16367"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.1.1.85"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class V-like SAM-binding methyltransferase superfamily. SETD3 actin-histidine methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.85" evidence="7"/>
    <dbReference type="EMBL" id="AJWJ01000001">
        <property type="protein sequence ID" value="KAF2078643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V607"/>
    <dbReference type="OrthoDB" id="441812at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018064">
        <property type="term" value="F:protein-L-histidine N-tele-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19176">
        <property type="entry name" value="SET_SETD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1420.10">
        <property type="entry name" value="Rubisco LSMT, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015353">
        <property type="entry name" value="Rubisco_LSMT_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036464">
        <property type="entry name" value="Rubisco_LSMT_subst-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025785">
        <property type="entry name" value="SETD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044428">
        <property type="entry name" value="SETD3_SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF47">
        <property type="entry name" value="ACTIN-HISTIDINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09273">
        <property type="entry name" value="Rubis-subs-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81822">
        <property type="entry name" value="RuBisCo LSMT C-terminal, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51565">
        <property type="entry name" value="SAM_MT85_SETD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SET" evidence="9">
        <location>
            <begin position="91"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="390"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="501"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="516"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="557"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2078643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="574" mass="64498" checksum="F3CE2AEEE06F0939" modified="2022-05-25" version="1">MIKAIAGIKSLIGEINETEKNLGMNCNYQQFERTFASVQKLIQAQNAMNQLKKTTTTAPVTSTKSDKAKEQELIDQFMDWMKKNGFDESKCSVKVGRNLNEGTGLVATKEIKEGEVFVEVPKELFITEQTAIKSLGEGILKERFFQMVPNMLVVLQLIKESLNEKSFWAPYINLLPKTYNTAIYFTLDDFKQLEGSNIQLEAMRSYRSSLRQYCYLYEFFANNPGFIQTNVFTWELFAWGVGAVMSRQNAVPVGTHYNQTALIPFWDFANHTPNGKITTYFEGPGITASALKNYKPGEQVFIYYGDRDNTHLLLYSGFSVKTNLNDNCLFSMQLENDDLKHDKIHILEERGLNDNMSIKFSPNPAVHQIPQEAIPFYRVASLNEKQTQSIAPKHDGGHNHHHHHHSANCGHDISLKKEAFDVLDEQSEKAAFTSLLNDMKLKLKSYPTTLAQDEDQLKQNPPAFTRFILYAKISEKKILERNIKYVESLIEKGVLNANLPIPDFSQQSSSHGHSHGGNDHGHSHGGNDHGHSHGGNDHGHSHGGGDHGHSHSGPSHGHSHGGNHGHQHGANCKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6K5</accession>
    <name>A0A8J4V6K5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_005737</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000237">
        <property type="protein sequence ID" value="KAF2072944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="21595at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903479">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00014">
        <property type="entry name" value="CH_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.920.20">
        <property type="entry name" value="Gas2-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003108">
        <property type="entry name" value="GAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036534">
        <property type="entry name" value="GAR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14149:SF19">
        <property type="entry name" value="CALPONIN-HOMOLOGY (CH) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14149">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN WITH IQ MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02187">
        <property type="entry name" value="GAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00243">
        <property type="entry name" value="GAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143575">
        <property type="entry name" value="GAS2 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51460">
        <property type="entry name" value="GAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="6">
        <location>
            <begin position="142"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="GAR" evidence="7">
        <location>
            <begin position="1712"/>
            <end position="1793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="36"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="272"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1351"/>
            <end position="1404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1500"/>
            <end position="1526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1543"/>
            <end position="1584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1600"/>
            <end position="1662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1849"/>
            <end position="2058"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="347"/>
            <end position="1226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="51"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="68"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="272"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="311"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1351"/>
            <end position="1369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1376"/>
            <end position="1404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1500"/>
            <end position="1518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1543"/>
            <end position="1577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1602"/>
            <end position="1612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1625"/>
            <end position="1646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1849"/>
            <end position="1866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1917"/>
            <end position="1988"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1996"/>
            <end position="2014"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="2021"/>
            <end position="2058"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2072944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2058" mass="233188" checksum="12146E1C96E050C4" modified="2022-05-25" version="1">MSRIPETIIKDSALPSTPIGFFERMKKQRSLVSLTEFPPEASPTEMVSKVADNTKSPDNRVQNTPIYSSPSSSSPSTTTTTTTTTSSSSSPLSSSSSSINNTSTSDLSASGAEEDEEIVIFKPKDTNRLTAKRRAWDDYAFLFNREEVRAWLEMCFDQSFEDEDLFEVLKDGIWLCKLVNLIKPGIVKKIHKKDGPPYMKLENINFFLCACMELGVSANCLFLPTDLYEKKNLKKVIYCLLALSIEGGKRGFKHKLMITHFNQTDAKPSIASASTSPFSNVNNNSSASLTSDGNSNATSAEDMPLPPTLVMKRRKKKGSKSRSSKSSITESSPAVIDPEEIERQRIAAEEAEAMRLAQIELEKEEAEAIRLAQLELEREEAEEKRLAQELEAIRLAQQAKEAEEKRLAQEQENARLAKELEEKRIAIEQENARLAQEAEQKRLAIEKENARLAQELEQQRLAQELENQRLAKEAEEKRLVAIEQENARLAQQAKEAELKRLAQEQEHARLAKEAEEKRIAIEQENARLAQEAEQKRLAIEQENARLAKELEEKRLAQEQENEKLAKQVEEKRLAQEQEHSRLIKEAEEKRIAIQLENERLYKEAEEKRIAQEQQHNILVKEAEEKRIAIEQENERLAKQVEQQRLAQELENARLAQEAKDKRLAQEQENQRLAKEAEEKRIAIELENARLAQEAEDKRIAIEQENARLAKQIEQQRLAQELENQRLAKEAEDKRLAQELENQRLAKELEQQRLAQEQENARLTKEAEEKRIAIQQENERLAKELEEKRLAQEQENERIFKELEAKRIAVEIENARLVKEAEEKRLAQEQENARLVKEAEDKIIAQEQENARLAKELEEKRLAQEQENERQRLAHLEALEAERKKFEQEQIAQRKRFEEEMEKQRIAQEEMEAQRKKLEQENKELMEKKLLEFEAEKKRVEMELAEQERLKREADLEQERLKREAELELAEQERLKREFEAQQIELQRLAIEAEKEKLALEKQRIAQEAEAERKRIEAEEIERKRVAAELLELEKLKLAQEVEAERKRLAIEMELQELERKRLLEEELERNRLLAQEQEERNRLLAAEAEKKRLADEQERIRLAAEQEERNRLLVQEQEQKRLLAEQLEEERIKIEKEEKAKRLEMEIENQRLAHEAERIRLEQEAEASRLALIQEQLDKQKQEQEFERQRIEQEEIERQRIAQEESERQRIEQEELLLERKRLEDMDKLIVANSNNGNELVNNIIVQESITNIVEITNIHVHNHLHYTITNNTNGSSPSKLQLIDELSSSSELRKEIFSEEEKENEHLINSLLSNQESLSIINSSLQSMEFLEEKKIIDIAQFNQYDIDVNNSSNNDSSNNSKSIQSSSKITEILSDQEETGSSEYDSDEYETDSEQEEEYEETSLNEMMGMIGSNQEDDEVLAEDETDILIKNLNDDSIDDFGNSESNSDILETSSISSCEEININNQQSIIVSNQSIEEESSSSSSSSSQSVLLKTSTSSLLESESNKSSSSKNSSPAKKESLLKKPIQVAKTLGILKTLSTPSSSSQQPLSSNNASLTTTTTTTVTTTTTTTTTNNNSPLKAIPASLIKRPLSQQSMLKPPTQIKIPIKPDIPSPTPKPITANSTFKNTMPSSDDDNINQSLTPTSSSPPDFDEDDSPIKMNLDVDIDLSSDVNDNAADQEKESNKEKFTNKKDYSFHRKYHTVANHPVYKPTLNDPTDKVFAEFLNVTMKKVLPPTIVIPTPVVRLSANKYKWGDTIVNMRCVNNIIVVRVGGGWMTLKDFLIRYNSVIQASDLDKMPENTSGTNGDDMTKLQIQKTFVKDGQVKTSQNLSPSRNNGNVVKATKTINAGGSTSPAGSQNLRSGTPAPFSRPMLRAGTPAPSDPSEGESEDIHFKLGIHRTSVSSGTLRTVGGSTPSKIPTPSKSSSSSPSLPSRSIASPSSRTTPTPSSKFSLSSPTKSKPAPSPSPSSGTPSSGNNVSSPSKPTIKKEIPRPSSSLSTSSPSSGTKTPTTTPPTTPTKTTSTSKTSTPRTSSLSIGSSQRTSSSAASPSKLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6L8</accession>
    <name>A0A8J4V6L8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_002718</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000078">
        <property type="protein sequence ID" value="KAF2075962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6L8"/>
    <dbReference type="OrthoDB" id="449052at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02205">
        <property type="entry name" value="CBS_pair_SF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF164">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="25"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="118"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="260"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="349"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2075962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="395" mass="44927" checksum="06BFFCB2F65C1C15" modified="2022-05-25" version="1">MEKFLNSHLISEMLDTQTDYWKKKKEKSLITLEEDAPVSEALKLLSSNFILSLPIKEKGRDDYLGFIDMNDILHSIIKLFLEKYPQQDRNKHWEFLLSEDNHNGKDFIDQQISSLINQSKLDNFIPVDENGTLFQLIDEVFSLGIHRVIAVDESATVKGLISQTDILKYILENLKEIGIPEFDKPVRDLGIIDKDIIFTNEKTLTIQTYYIMLEKNAPCIGLTNDKGEFIGNLSITDLRGLAPPSFRTLLYPSVEFWNLHSKKKKCKVSTDTSLRDVLNLLYTNRLHRLWVVDEGKHQKCYGVISLSNIMRYFSRYHDKKAPSFLSKSTSSCATSRLKSSTNKFSPLITYTSPTTNSPSPPALNLDEAGEPAAIIKEVKTQSKVQSTPTITPASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6P2</accession>
    <name>A0A8J4V6P2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Adenylate cyclase-associated CAP C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005619</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000229">
        <property type="protein sequence ID" value="KAF2073064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6P2"/>
    <dbReference type="OrthoDB" id="2522835at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013912">
        <property type="entry name" value="Adenylate_cyclase-assoc_CAP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016098">
        <property type="entry name" value="CAP/MinC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036223">
        <property type="entry name" value="CAP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004027">
        <property type="entry name" value="SEC_C_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33747:SF1">
        <property type="entry name" value="ADENYLATE CYCLASE-ASSOCIATED CAP C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33747">
        <property type="entry name" value="UPF0225 PROTEIN SCO1677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08603">
        <property type="entry name" value="CAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02810">
        <property type="entry name" value="SEC-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69340">
        <property type="entry name" value="C-terminal domain of adenylylcyclase associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103642">
        <property type="entry name" value="Sec-C motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Adenylate cyclase-associated CAP C-terminal" evidence="2">
        <location>
            <begin position="87"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="307" mass="34592" checksum="CC042B49567A7187" modified="2022-05-25" version="1">MSSSETSTAKTSNQVKDEVSAQVIADHMLVSDASKSLPQPKGEESIKMKSDLEDKKADANLGSGAKDQEVYFLKDENEQTKELCKNTIKNGNKTTVYFKNCKDGVYTISARVTKIMIESCQRCTFNFKGRIFTNTIEAWKTVDLKFNILNDQIKTLQVDMTSRINISYDTRSSLHSIIWSGVNDMKLHFQDEPDFVHATGFEQMKSQYPEQTLSFTVDQFTTRIIKGELLTEQIVRLANGYPTTEREAREFDANSEENAKRAEEYVRQKLKENGVNLGAIGIKKAEAGRNEACPCGSGKKNKKCCNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7P8</accession>
    <name>A0A8J4V7P8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lipoprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004507</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000160">
        <property type="protein sequence ID" value="KAF2074189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7P8"/>
    <dbReference type="OrthoDB" id="19902at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040310">
        <property type="entry name" value="DDB_G0292248"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31648">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25544">
        <property type="entry name" value="Ependymin_amoebozoa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035253812" description="Lipoprotein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="227" mass="24375" checksum="E524F1EF52EEB0FC" modified="2022-05-25" version="1">MKFLTLAFLMIMVALASAGCANPSGFQTTFTSNSFNVAGGQSAYESGIVNIDFVGQRASSVFEIDISGGEKGSGFTWNFGANQTSYYLSPEGQCYEASSSAQIPSGFPKVLKDIGSLTLGKFPVEMLEVESDTPALNQSVVFDLTNCVMVSTYLVNADESNPGYGTINFFNVGVPSDSDFQLPQVCIDAVGSSRPKIGFNHKRRNVEAVSPLSKTLQYTLNTFKIQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VAM6</accession>
    <name>A0A8J4VAM6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001375</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000032">
        <property type="protein sequence ID" value="KAF2077309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VAM6"/>
    <dbReference type="OrthoDB" id="19854at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="806" mass="92818" checksum="6E709970B3470B8B" modified="2022-05-25" version="1">MYSFDLKRKFENEGFDEEIKDTFQFYNNIYNQYPSTQLYSNNNNSNNNNNEYLNNNNNNNINIYNSYQIKKKIKHDNTVSSLQQRNEEQDYFNNYLSVELIVKIFSLCDPFSTPLLYVCKYWYQILLDNQTSLWRDIRVSISDKKPFSYTPKCESIISFFKTGYAKGLHHFSITPSLSTAPIPSSSSSSSSFYSSSSYSRSRYRDYSQRLPSPPHCNYKTTMINLDDNNNNNNSNNNNKSNQQKQQQQQQHVYRDIVNFTKIISENLTCCLKLLELNLTVPLNNQQLYNISSHCKYLTSLNFNSLLISDQGMRQSLSVMKNLQNLSIMASGNVNLEFIKRLDEPWTPKSTISPLTLHSIATYQKNLKSLKFSLLPHFTNQKELLFILSQQQQQQNNNNDQDNHQTLQQHNIIGQDQSLGNNTSMESMMILEQSFSSLCSLKNLHELELINCNIPNHILDNVLEQLICLEKLVLYRNANISLLKFNSKTLKHLSISSLSMLNTIDIESDSLEEFSSEGCESLTMTRLEAPNLVIFSFDSCSGKIKLPCAHKLSSLSLFECRDLSQKSLERLLDSLSNLKEFYVYLQQIENLRICSSTLKKLDIEAWHGIVSCILDCPKLEELKASESSLETIFLNADHVNNIELDFCDSLRTILINVLSCDNFSFTQNHPSPSSNIDLDHFNHFDSLNGSNTKIYDDNDDEYDEYDEDDEDEEDVQVDQQENIESSPSIEAINSRNRNTCSERSNQSFFSPVSLFFQSQYFIQNCTILSGNIVGKLSSKVIGSLSIDHKSLKTFVYDTNKVLKLTSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VB37</accession>
    <name>A0A8J4VB37_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000019">
        <property type="protein sequence ID" value="KAF2077804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VB37"/>
    <dbReference type="OrthoDB" id="20439at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="186"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="220"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="378"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="442"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="459"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="982" mass="108414" checksum="80BE8AB15C1A3F74" modified="2022-05-25" version="1">MNGQSDSSKMETTGTSSSSSASTTATTTTKPIKKTSLGLSSKSSSDSSVKKISSSTSTSSSSSGSSALKSKTSSSSSSALRTVTRPITRSASAASSPTSSPTSSIPTPTTTTTTTSSSSPTSSTVLKPGQSMSNITTRRPFLKKSSGLKTAPSLTSTTSSSTSKAATTKKSSLEKRSSIEERSEYSSRTTTTTTTSSSSSSSSSSSRSTKTIIDDENGLYSSSIQYQSSSSTKLEEFNPDTSPDFEKVVSDFIILDDVVPNYLENLRLQEDDGSGSNDQYHLKSEQIINPPPTSLYNNQLPPQDIVVGDEEEQAEEQVEEVQEQEEIEEISRDQKVIQEEKPIEIKKTTSWMSSFFNKVLPLSGMAAKSATIATTTISKSESLSASSPVSSFEQSSTTTTTVYQSTESTIKPPSPQPYIQPQQPQELKTLPPPPLMNHRHSQQLYNPMYQSSPNLFGHNNHHQYHHHQQQYHHHQHHHQQIRHSQQYSSSPNFNYLMLPHNQPGGLALLPFTIGPGKAVSIFQDWVKSLWFAPADFKEKLEQSLQLDHYFVPYFAFSSIVHSVTSGKVGFLKEQETVVVTSSERRLVQEEIEWMKASTNPYTQTHNDILVYSPIMNGAPNATTSNPLDPPIYENTKEIAEWRLDVSEPFHAQHPQLLHPIVDQQISIYSPNPAAAAAPYQPNPKDPLFLANNENIQSALNGKLVKVIGKDDWETAWTIAERKIKRNESYGKDSKLKRDFKCHSVSDVETETKFENIQYRTVYVPIYKLTYNYNNRDYEYIINGQSGKSHGIRPYGAGSIKNTAIKFLTGGVFSSEKIIGSSGGDDSSNNNMESTALISTNSNAGNNNNSNSTTTIQTGTFVKGADLNQQDIHYNEDEEQVLLYRDDLYYLLFPSSDQFLLYQMVGYIVLKNIGDQDILLESHKRMSDIVGGECILRVGETKTFSYKGSWCIEIKQGLHNCLQVVRIETNSGGDKPSLELSMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJQ3</accession>
    <name>A0A8J4PJQ3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Clu domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010553</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001190">
        <property type="protein sequence ID" value="KAF2068122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJQ3"/>
    <dbReference type="OrthoDB" id="18768at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025697">
        <property type="entry name" value="CLU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027523">
        <property type="entry name" value="CLU_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601:SF24">
        <property type="entry name" value="CLU DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT EIF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13236">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51823">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Clu" evidence="2">
        <location>
            <begin position="253"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="570" mass="63523" checksum="4D29C828BCB53EDB" modified="2022-05-25" version="1" fragment="single">MSDNTGFKSILAYWSVKDKNNKTTSINHNYNNNSIRNNNNNNNNNNNNNNNNNNNNNINSNNDYNIDNNNHNNSSISNVDNSDDNSQAYHSNTLYRPISSSSSSTSDQQQQQPSLSPTSNAASVSTYRVLMSSNVLSSDESNSNSNYYPQSTTSSTNQTNYSISSYDNISITPLSTATGTTGDNEQQILNNNNQNLMGSMIYYPKSISNHSYSSFDQNYLVGDSVGSRSAYGSSYSSNHYLASSYSSSYTSAAAITPDIELQIKERYLAETRFAKAKGRDWNKEFQSLLRLTPCLEKFQKLTFLAMDFVSAAENYGFIIIKEMFMPDELKTIKPVSLGGIAGGSKYICQGILFKFAYDQQIGDQWLYGGRFPSEVGACKAAGNELKGLSCFFQHVLDNEYADKVNVPLMCTIDYYGYRLIAMSVLPITKNTLVYGSCDGGNKVHTDITELNDIMEKVGKSMNLRGHLSGINPQFVYGPGDLEIHKASDDQYYALDFGRLLVPEAPLNSIGQEQSYRDIFFKLLRPELLKNLNTPLSSDAFSGWSANDPKNEEYNNDIVKVTNQLYNDIIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJW9</accession>
    <name>A0A8J4PJW9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009564</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000743">
        <property type="protein sequence ID" value="KAF2069115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJW9"/>
    <dbReference type="OrthoDB" id="24009at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1141" mass="130102" checksum="002294D702048765" modified="2022-05-25" version="1" fragment="single">MIIYDDQWNTPTCNKVWKVEVNKRFTLEVYFDRSGIVKKEKLVKIPKGTIALELKIKGSGSPHRKGADKNRYVELDIGLIPPTVTRIDIQEKKCRYRPYVLYEGEWIPDSVTMLSIPSLPFPLSLIPDNVKYLKVQQQLEEIAETSMVPPSIENLWIESLSRNYTPHDIPNTVKELVLGSCSLDDMHITPTFTSPIHINDLKVYNDEEIENTSTSDDSIKIDILQNMVECNLNVQSIVISRPFTLVNLPPTLTSLIITNDTMFNNSLSSLDPLPPQLKYLSLPSSYQQPIYQLQTHIPTTLSHLELTVTVYKTTFTNDWLGKKGYLPLSIIHLKVNLEINSIGEKDGQDCDETLSSGLQIILPSSIQSVCLVPNNEKYRKHVSIIDKQDYYSTTPLLPKIREGLKELVLPKKFNQVININSFPSTLRSLSILNPYYNHQLNSNNLPATLTSFKCVSDMIELDLSAYQSIKELSVHGDSIINYPPNIETLDLQLKEEDENYEHYYHSNHNSVEEEYQFNFGNTHLIKNLTYRSGSQSNLEKLLDLLPNVQSLWTHDIYLNVPSSVTELKSFLSRISYHPILPNLKHVHLDLETYVLNIPRVVNNERDEKKKSFIPRQLPMHDTFFKLWRNVYLKPMILEAFCRSPLIFDGKTGTLEHMDKFKHYNIIIKNKEQLVPALSKFDKADEIYIREKGEKDDLSKLISRSIKKLRCTFTTGIPKWISHLTLDNLNSFDYFTSEIPPSVTNLTIEVINPGDTKSFPTTIKYLTLDKLDSFYGTCPIPPSVTYLTLYNQLFNQINQDDIPSTVQKIEFKKLVFESYSVRSYDIPSYISSRLVNGVFYIYSKSNNRTTIPPNTTHLFWADSEQYIDNDGDGINMPPSVHTLVLGQTFNSAILSLPPTITQMIFNGRFKKPLKFISFPPALKYLSFASLDTLPQENELPNGITHLSIGDTQYFDSNSVPQSVHHMQSSFKGYDADQIPPQVKHLKIAGCGNQIYLPTTTIKSFVTSPYDKIIFRDHNSQRYLAQNKSLPFSSSAHTIDLIDNTSIMNVPYTLGNNIKSITFGPSINKVLLKGAIPNSVEKIMFGDKFNHKLNKSILPTSLLTLVLGRDFNQDLNDNIPSSLTELVLNTKSKPSFTSSSQFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKH2</accession>
    <name>A0A8J4PKH2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_009240</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000661">
        <property type="protein sequence ID" value="KAF2069447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKH2"/>
    <dbReference type="OrthoDB" id="2306at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000102">
        <property type="entry name" value="Actin-interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000167">
        <property type="entry name" value="Actin-interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19856:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19856">
        <property type="entry name" value="WD-REPEATCONTAINING PROTEIN WDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="54"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="186"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="231"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="317"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="477"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="518"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="561"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2069447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="595" mass="63533" checksum="FB759AD84D946C31" modified="2022-05-25" version="1">MSATLKTILSPSPVVTRGKSVFVNGDPKGNNLLYCSGNAIIIRNMNNLLEADMYYEHPAATTVAKYSPSGNYIASGDVQGNLRIWDTINKEHILKITIKALSGAINDIAWTSDNQRLIVVGDGKEKFGVAILWDSGSSVGEITGHSKQILSCDVKPTRPFRAATGSEDFVANWFEGPPFKFKSILKTEHSRFVNCVRFSPDGEKLVTVSSDKKGVIFDGKTGEKLIDLAADGAHAGGIYCVSWSPDSKQILTASGDKTCKVWDAATGACTKTFTFGSDVNDQQLGCLWQGDSLLSVNLNGDISYLDETNTARPKQIVKGHNKLVSTIAFDRATGALFSSSIDGVLLQWDLSTGLASSFSGPAHKNQITSIKVVDNKVYTCAKDDSAKISSLADQTYGASIGVDSPAVGIAVSGDLTVTASMKSVYVIKNGAIVSTTPAPYEPTCISTNGSQVAVGGKDKKIYIYTVSGNNLTQSHTLDAHRGELSKLAYSPSGALLAAGDTNREVIVWDGKTQKSSSWVNHTGRVNAIDWSQDSKYVVSGGLDSQIFVWDLEKSGPLLQIKNSHKGGVNEIVFTGANSIASAGNDNSIKIWNIQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKT7</accession>
    <name>A0A8J4PKT7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_009036</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000623">
        <property type="protein sequence ID" value="KAF2069642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKT7"/>
    <dbReference type="OrthoDB" id="2162994at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051140">
        <property type="entry name" value="GATA_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658:SF75">
        <property type="entry name" value="GATA ZINC FINGER DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="6">
        <location>
            <begin position="391"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="152"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="205"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="284"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="162"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="246"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="288"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="304"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="330"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2069642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="454" mass="49491" checksum="CD38A24ED22A14A4" modified="2022-05-25" version="1">MTSSSLLYTSHPSLTNVMSTCNINNNNNNNMNNSINSSLNSSNGINNPLNSSNGFDISNIDQCLKHCGCRNPKELIQLIRSDAIENIELSWPDDMGLWFVNVIKSHEVSLQNLVEGIKVTGQILLVDPTSARFDLSKKQKTILIRCSNINRYSSSSSSSSSSDDDYEEEEELDGEVVKSPQSKFSLLLDESEKLRKSFKSSAPAFFKPKNSSGGGGNSSPSSPPTKALKYHQSGAAGGTHERYTGKRSSSNNYTATATNQQDQDLDASWIEDMKKNNRIELVPDLSSPFYSSNGMTDSGCSKRSPSPLLGGSSCLSSSSGSSSPGLPIETTDSPPGVVSNSGSATPDLTTVDSAERAILEGQIHLPPLLRPRQYHACKIPKEDRPSKRRKNHTSLFCRHCGTTDTPEWRRGPDGRKSLCNACGLHYSKLVKRENMAVPQLSRTFDISELLNPTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKX3</accession>
    <name>A0A8J4PKX3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ComC supersandwich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010254</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000998">
        <property type="protein sequence ID" value="KAF2068419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKX3"/>
    <dbReference type="OrthoDB" id="19767at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="2">
        <location>
            <begin position="2"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="290" mass="32608" checksum="614C0D7A725937DE" modified="2022-05-25" version="1" fragment="single">NTGNNITTDEISFAYYISITELKELDLNGGTVQHYYLKDWTFTNVSTTVRKEYQYSTNITNNDVSTEISVNLRFYDKQEQVIFANNEYFMSPNSVKYIVTMTPYGFSSRLNYLELVMASNFTLNSNDTCSSISKGDSSINAEYIQIKVNDRYLYSRFIKLGEIDSKPQQIVNKFMDNKDQQTQTSAVSYVGVQIPFYTKSVLLDPDFSVLIDTKPVTEDDDDAVCGDKLKDTITNTGSKGLTKVQIAGIVIGTVGFAAVVAVSVSYAVVKNKQSTKARKNMQVKLENFNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL63</accession>
    <name>A0A8J4PL63_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_010087</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor Fz/Smo family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000918">
        <property type="protein sequence ID" value="KAF2068584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL63"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050949">
        <property type="entry name" value="GPCR_Fz/Smo-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31787:SF15">
        <property type="entry name" value="FRIZZLED AND SMOOTHENED-LIKE PROTEIN P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31787">
        <property type="entry name" value="G-PROTEIN-COUPLED RECEPTOR GPCR FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="95"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="97"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="131"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="163"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2068584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="176" mass="19450" checksum="C4CAEF0174F5DF9E" modified="2022-05-25" version="1">MLYLLFTITYNSFFKFYLNEDSYTQLLLDWVVCLTTASDSKSCILHLPGFIYRYIGLISTAATGFVSFLVYAIDINTFNIWRESTPVRYILRKCGVSSSGSSSSSTNKSNSRRSRGQSLGPKMNHRMSILQTQNSSTDSQSSNITTPDTQGSTVLSIAASGDSIDSTPRNASEQQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLB3</accession>
    <name>A0A8J4PLB3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_009552</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000739">
        <property type="protein sequence ID" value="KAF2069127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12087">
        <property type="entry name" value="TM_EGFR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035178611" description="EGF-like domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1304"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="5">
        <location>
            <begin position="424"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="4">
        <location>
            <begin position="786"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="3">
        <location>
            <begin position="1074"/>
            <end position="1278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2069127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1346" mass="150522" checksum="6B2FA9E05D0FDF81" modified="2022-05-25" version="1">MKLSYIYLLFVFLFLCQGSLGQALYAQVDPILPFGIEPYAGSNGICFFDFYVNNFFDLDVVSNYVSCNILLPVSVTTTCQMATIRTSHSTTTTLVKTTMTSDSSVFNNIQLQIILFNGTSFSVNLNRTSEVPLTYNCKTIPENLAFTHSITEARLLNTSYSISTFRFQSYLTLDPAYERPIPDELFSILPTVGFKINTRYIKGSTYLLTFDFLQGDPGPAFLLAFMVKLGSLGSYQVTVPLTYGKYPNVAMDSKVHYPFKNTPNIGNYLQYKSVDSSMNVAPVFYQDFTPLFGQSFANFSIYFKNTAYTDITGQYVMGNSWKNLGVIFARTTYESLSFSFVYPKPTYLTFNIDTFKNYHYRFKFYFDEMDTPNYYYYPFGMINTLANSITYQYQVFLKDYENLLILNCGVETVTQYPNNANNGTDTMPPTVESYSLLPLNETLSVLRLELSDNLSGVYFAKLITDGHVYYIKIENLVMGDIVGGTYELLVPFKKRTSFNLTIYDNAGNVASYSNDLLHYHMGVPQMVGEPLLASLLSTFYFDKNSVSVANSPVDVVLYADLVESGQNKTNRVDWNSLYLKVFISRLAKPLEVVGRFNTSKQLYQFPFTIPQKLMNGNLNYSLYINEQQVNSDTLIGVFGSQAQVFIDNPGEFDMMFPIVTQFLFSPNDTTSSSPLLQFSLTLDEPMKKVVVQIVGDYDHTGKNYTFVDNEPISINFQVDSARCRPQFYFIRYMYTEDLYGNKGEYIRHSSTGMHQFYKFPDLDLPQDVYYYPYISCPSTVVDTNPPSISSLFVQQVSDIHGQQVNITFIANDNTGMSSQDIPLCVFHARNNNYFAANATILLSGPNSIKFQCLISIPIGVPPQLLLSIYGIMDLHFNYIGFTAIDLGAEGQFTLTPFTKPIISSLSFDNTTNTVILTGHNFAASLSGVNITFDGTSSQIYNGISTNTTIILTNIPLAYNYSIQVIGADHSLSNSVIFIRAVPTDFSSSSLSSSDSSVGTPPPSPTPVVGCKSDCSVGQGYGKCVNGGCVCNSPHSGLDCKSVVDTKPVIKPNPVKPSVNITIPGTSSSESPQFTSFIYVVALRELDNTDTLINNYEFNSDKWILVNEGSFSNEQVTTVAYKYLIDNSYNTTIVSTVQVFEKETNVTFGNQQLFMNPSTIKFTFNITSYPFTKSTNSLQLVMTAALESTEKVACSYKEFVDDENSSQYLTLQIEDRSLFGRFIKFGMIDGREQVVTNTQLDNIYGGKELSKSTSDQSYIGLNIPYYTKYALLDPDFSVLIEQNTARDQSNSICTKESKKLTNAQIAGIVVGGVVFILILAAVVIYRLSISSKGSFRMAIKLRKIMGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM67</accession>
    <name>A0A8J4PM67_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycosyl hydrolases family 39 N-terminal catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_009153</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 39 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000645">
        <property type="protein sequence ID" value="KAF2069528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM67"/>
    <dbReference type="OrthoDB" id="15153at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1500">
        <property type="entry name" value="Glycosyl hydrolase domain, family 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049165">
        <property type="entry name" value="GH39_as"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049166">
        <property type="entry name" value="GH39_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000514">
        <property type="entry name" value="Glyco_hydro_39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051923">
        <property type="entry name" value="Glycosyl_Hydrolase_39"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12631">
        <property type="entry name" value="ALPHA-L-IDURONIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12631:SF10">
        <property type="entry name" value="BETA-XYLOSIDASE-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01229">
        <property type="entry name" value="Glyco_hydro_39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00745">
        <property type="entry name" value="GLHYDRLASE39"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01027">
        <property type="entry name" value="GLYCOSYL_HYDROL_F39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035306243" description="Glycosyl hydrolases family 39 N-terminal catalytic domain-containing protein" evidence="5">
        <location>
            <begin position="21"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolases family 39 N-terminal catalytic" evidence="6">
        <location>
            <begin position="39"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600514-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2069528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="494" mass="55791" checksum="3E53DD08C5136411" modified="2022-05-25" version="1">MNKTSILLITLLSLFVIVQSQSCSSSASVQFTADLKSTTEWVHYWERCVGSGHALLGMRDDWRSALKIAHDKLGFQQVRFHGIFDDDLSVVQVDASGNIVYSFYDIDQVYDYIVGIGMKPFVELSFMPELFASGNATIFHYKGNITPPKNYTLWSDLIVNFVNHVIDRYGMEEIQTWHFEIWNEPNCGFWSSNQTEYFNLLKVTFDAVKSVSPTLSVGGPATCQSQWLPETLAFTQENGIVLDFISTHEYPTDIEPVQRNIMNQVISKSRSIVGPDMQLFYTEYNDGLFSPQQLHDTTYASAFAIFNVIDVYGLPDLFSWWTFTDVFEEQGQQSVLFNEGFGLQTIYNIPKPSFKAFEILHETGNLRAATTGSHPTVGVLVTSSDEEVMVIIYNHNIPGAPIQNETVCVVVNNVPSNARFADIRRIDDTNSNPIATWQAMGSPNYPTPNQINQIYQSAEFKVATIQYKRLTPSDIQFDNLVIPPQGVIAIKIYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMD7</accession>
    <name>A0A8J4PMD7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Myb domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_008984</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000612">
        <property type="protein sequence ID" value="KAF2069698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMD7"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17997">
        <property type="entry name" value="DEXHc_SMARCA1_SMARCA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000082">
        <property type="entry name" value="ISWI chromatin remodeling complex ATPase ISW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000101">
        <property type="entry name" value="SWI/SNF-related, matrix-associated, actin-dependent regulator of"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.30">
        <property type="entry name" value="ISWI, HAND domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044754">
        <property type="entry name" value="Isw1/2_DEXHc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015194">
        <property type="entry name" value="ISWI_HAND-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036306">
        <property type="entry name" value="ISWI_HAND-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015195">
        <property type="entry name" value="SLIDE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623:SF49">
        <property type="entry name" value="SWI_SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY A MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09110">
        <property type="entry name" value="HAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09111">
        <property type="entry name" value="SLIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101224">
        <property type="entry name" value="HAND domain of the nucleosome remodeling ATPase ISWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="305"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="601"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="10">
        <location>
            <begin position="964"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="231"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="858"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="919"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1140"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="25"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="86"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="103"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="155"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="869"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="919"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="938"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1202"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1238"/>
            <end position="1247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2069698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1247" mass="143430" checksum="B42DCD13E772BD9A" modified="2022-05-25" version="1">MDTKSPPSATATVAAVNSENKDKQPQQPTATTTTTNNAPATTTAMDVDVNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNKSNNGSINGDSKVNSLNGSVVDQDEEEEEVEEDEGDVEMDEQEDEDEDEVHPFFDEDDTDTATATSPTLGGINRSDETEEEKKQRLEAIKLEKDRLKSVREQQKKQLAEVEAKQRQQLAEDKEKSAKDRLKFLLERTEIFTHFVSKNNATTATTSGKKKSAANAITSTPTKRGNTTEKEEDEEIMKEAIDEEEPHSFNFFTSNPPYIKSGTMRDYQIYGLNWLIQLYERGINGILADEMGLGKTLQTISLLGYLSEYKGIRGPHLIIAPKSTLSGWTKEFKRWCPFLRVVRFHGSKEERDKIKKEQLIYKQFDVCVTTYEVAIREKAAFKKFSWRYIIIDEAHRIKNENSVLSKGVRLFNSQFRLLITGTPLQNNLHELWSLLNFLLPDVFSSSEDFDKWFDLANTENQQEVIDKLHKILRPFLLRRIKTEVEKSLPPKKEIKLFVGLSQMQRELYKKLLSKDLDAVVVGSKGNTGRLRLLNICMQLRKACNHPYLFDGLEEPPFTTGDHLIENSGKMVLLDKLLNRLKARGSRVLIFSQMARMLDILEDYMLYREYKYCRIDGGTNSVDRENYIDSYNQPNSEYFAFLLTTRAGGLGITLNTADVVILFDSDWNPQCDLQAQDRAHRIGQTKPVTVYRFVTEKSMEEKMVEKAEMKLHLDAMVIQQGRLVEANKNAKPEELLAMVRFGADDIFKSKSSTITDQDIDAILKKGEEKTELLNNKVKDLANNPIKFTSDGNLYLFDGVDYKSQQTATGLWPEVLKRERKPNLENNEEFLKMVGGGSGPSKKVEKKPERKFKPPKQPIIYDFQFYPARLQQLYDKETAAYNKKVEWYDAKEEREKNNPTSVDKDPAAAEKEDEEEEPNFDDLTKDELELKEKLLKSGFNEWTKTDFKSFIRGCELYGRKSYQNIAETIETKTEKQVLDYSNVFWKRFKEVAEFEKLIARIEKGEERILKYKETQDSLNHKISMYKNPWQQLKIQYGIKKNKTYNDEEDVFLVCMMHKLGYGAWDELKEEIRKSPLFKFDWLIQTRTPQELKARCDQLLKYIIKEHQEEIEAQKEKERKRKEKEKRAAEALSGTSSSSKKSSSKDKKEDASVLGKRKKESTPPPPPAKSSKKGAAKTTTTTTATNGKSKVQTPETESEKEEDEKPKKKGRSTTTTNSRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMY6</accession>
    <name>A0A8J4PMY6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dihydroorotate dehydrogenase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_008464</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000524">
        <property type="protein sequence ID" value="KAF2070218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMY6"/>
    <dbReference type="OrthoDB" id="14784at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004152">
        <property type="term" value="F:dihydroorotate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02810">
        <property type="entry name" value="DHOD_DHPD_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050074">
        <property type="entry name" value="DHO_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109:SF1">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (FUMARATE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (QUINONE), MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydroorotate dehydrogenase catalytic" evidence="7">
        <location>
            <begin position="136"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2070218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="371" mass="40479" checksum="678E2E7C76311BB0" modified="2022-05-25" version="1">MTDIKWDINKPIYDIKKSWEDNLENGPFVQGYQHQNRVKVEQDKYIDFLGHKLASRLGVPAGPLLNSQWVKFALDAGFDLPTYKTIRSHPNLGHPVPNVLYLDLEGDDKKQLSKHDSGSMLHATSTLPHNMDQLAITNSFGMPSMSQEYLHKDIKLANSYLGQGQAMIVSVVGTASTAHDFVQDFVDTASLAADAGAQIIEVNYSCPNVVTGEGQIYHNPDAVYEISSALTKCLAPKNIPLIIKVGVMEDMDKMTQLFIKAQEAGVAAIAGINTLSMKVTDKDTGIPSLGASRLTSGVCGAPIRSAALDWVSSARNIIDKQNLSLKLLGCGGIVKPQHFDDFLNAGADIAMSATGLMWDPYIAMNYHNKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN59</accession>
    <name>A0A8J4PN59_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007685</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000424">
        <property type="protein sequence ID" value="KAF2070990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN59"/>
    <dbReference type="OrthoDB" id="17033at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF132">
        <property type="entry name" value="PROTEIN-LYSINE METHYLTRANSFERASE C42C1.13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="242" mass="27672" checksum="B22C31FDF8CAD8C0" modified="2022-05-25" version="1">MSDDEALGLTDIFPTVVELEKYKYNFYDNVVIEIKGQELQNVNVQPSTGLLPWPASTILSQYISKFNDNFKDKNIVELGSGVGICGLVASKYASYTVFTDGDPQSNKLLFDNIKDNNHLYKNNSNYSNQQPPIELLSWGKTSSLEDFKEKYNDLQFQVVIGSDLVYVDENIDPLFYTVDKILSTSSTPSPAFYLSFLDRKNHLPTLQSVSEKYNFTMETIDLDTFLDYKPSTMSRMFVFKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNC6</accession>
    <name>A0A8J4PNC6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009365</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000689">
        <property type="protein sequence ID" value="KAF2069319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="832"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1363" mass="155378" checksum="68C41181A3A3BDF4" modified="2022-05-25" version="1" fragment="single">VGKQQQQPNFKKRKQDNDNVYDDIDNHSDDSEIEIKQPQNSNTTNEIRTFTLEKGYIKSNITKLDIMLENPSYSDIILGDFIPDSVTHLEIPILYAFLCPLIPKSVKSLKLKSQITVYDGAFRMIPPYITNLSVFIVGDWNLPFSDIPPTVKKITINKLYPFANLNSISYMENAFNIALHYNMDEDISIISSIRSNTINPAIQQLMKKDNVFVKCEGSGAIPQTTKHLVRLCRDTDPLPFGITQLICSQDRAILPGELPESLIKINFINLNQCLSNVHLPLSLKYLIIPNMDQLIYRNTHIPKSVTHLGINLNSSNHEGIFRNSWLFKKGYIPESVRFIIINIYNLKPNQISDIILPSSILYFDLQVEEAYLDKLELFYKKIKFIDAQEYYHNAPEFVPTTTLETGNISKIDFGKYFNQLIKPGQLSSNITDLTFGQSYLQSIGEGVIPSSVDKLTLECKESIGAVSIPYPISSLSLPAILLPDNPLVDKTSIKDLSIDGKLNRVDFIHIEDEDEYMEHDEVDQTPILIEQLPFSSLEKLCLKNVKNIGSESMTDYSFPHLQELKIDTIYPDTQRVYVDFLSKVMVNLKSLFLYGNFKSHLKNIDFPSTKLESLDANEFFSINPNQIPLSMKNLSIPINEKNFNLIQSKNFVQPKIKNDNQDSLKKRFQLQDMMAHSFIYHDYRSKIQDIKRDGSRFKNLSIFTETGIKGLDEIPSNVIAWYFSNRTQSLPPIIPETVKKTACGYYPTIQIPSWITHLSLYYGDGKMVKKGFLPESITHLELDTLNIASQNNEFGFFDYDNEDQDDEDDEFIDYHNDFDDSRESQLVGALSDSDDEYHDDDDDEQLNQPDDEDEDEDQNDEIEAYFLTLKEALPSKLRVLNIFNLKFGVCSIIPPTVEILQIRDSRFKLVDKSFKNLPLTIKKFKIPKNCFHENVKKIPSFLATRLDGNYQVYEKHVPIGKKTSHLIINGPLVFKKGLIPNNVNTIIFGHSFNQPIPHLPSSITEINFGDGFNQDLSTISFPPNLKYLSFGYSFDKPLDNLPTGLKFLNFKVLHSAQSLASIPDSVDHLSIKYDFLDSDSDVEDELNNDDEDDNDDSLSPSNPLLVIPECIKFLKLGVSIENVKLPLNLKSITCHHICLGENKLKTSEKKVSETIDTTLSLENIDPSIKLNINTTGSFDKLINPNSLIDNIKSISFGTNFNQVFPKNSLPPSVEYLKFGFYYDQQFQIGSISSSVTQLELGTEYDKSLKGILPNGLKKLVIKKGFYHKDMLDWLPKSLEYLELGNDETLPSPQPFPIEKLPTNITTLILNDSVIISSPSKIPKTIKHLKLCKCKTWDYLPIDSLESLTLGEYNYPLNMIFKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNX7</accession>
    <name>A0A8J4PNX7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nucleoporin Nup133/Nup155-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_007229</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleoporin Nup133 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000373">
        <property type="protein sequence ID" value="KAF2071455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNX7"/>
    <dbReference type="OrthoDB" id="103454at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000972">
        <property type="term" value="P:transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037624">
        <property type="entry name" value="Nup133-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13405">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13405:SF11">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117289">
        <property type="entry name" value="Nucleoporin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="16"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2071455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1108" mass="127083" checksum="D9A1FE82B71A54D4" modified="2022-05-25" version="1">MYYSKSASAVGRKKVSTSPSDQPSSSSNNPTNSNNTTGSGNIQLDISRKTKENYSIQTIYDLPFILRETIKELNSDPSYNNVTLTGSITYEGLCYVVLLNDLFVWSIEKPQSCSRLNIPIDEINNQQTNQNHIVVGRSKLGQDKFTVFVCSPNGSFVRYWANSSKPNVTQDVTLETTVSNTKVNVYASDCQPYGIIVGNSNGSIHLVNIKQNILVAKKINKSQGMLSYLYLSRPSPVLFIASSQPSTSSQQQYSSVVNANNPSSLHTSTTRYTFVLTESSLLKYEISNNSEKIVIDYTIFKEVQKHFNNSMDLKFHQMYIDTMNDQNNEEIQILNMIFYETSASLNISKYYLIVVEIQDNQVLRVYKNDNFIAKKPITGGGIVSYEPRVSSISNNKHYLCWNDSVFYSSKSNGEIKFPERFISGSGVYNQEYHLISEEGIQKLDVAPFGVQRSSSLTSNQSTDNNTSNVHDQKPIMFEVPETDDMLSIKRNTVLKALNSISLKQQNKLNQYLDTCKNYQDLDSVIQQVSYFIIDQQPKSKFWADDAKLLGDLGGEISVQLKQQLEDKKKRHSFFIQFVEENGIKNLIEKSTLHLIEKNQVKISAAIELRNYQNQQEDKSKSSSGGASFSILPKLIQSLVSDRLPNWTNIGMNVYELFYSYVSGIDQILLCIFDKLDHQQQKQSNSVQILKEMIEGTDIFLRILTVYPIHLLDSPVLPNSENIHTLLSQVVYLLSTFIQIDLQTSATQILSQKDLGIPNVENALYDQLYRLTNIYLSSLKENEVEQFKNFKTRLIDPFVSSGRFNLATTLANKYDDYHSLINIYIADKEKRDIQNLKLVECLTKFQALGLLNNVFEYMLEKNLKAEILELPDTFNEPLYKFLSKYPDLAWIHSIRMKKWNNISNVLYNESLKEPDYLSKKEKYLSIAKISMIIDENSRLSEQQKDQQMEIINQNLNLIKSQKTFLPDIRNVLSITNIIEHILQRSNIESMDNYLICFNIIAESNLLLKEDESLDLYRHILGLAFSNEISIFCTTPNSDLQFDYGLKETNFYQLLLKLPSNDNIQEEFVVPLLEEFYKLKVKLIQDPSEKRQFQIGLTRILELYKEDNMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP96</accession>
    <name>A0A8J4PP96_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_007405</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000394">
        <property type="protein sequence ID" value="KAF2071268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP96"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04138">
        <property type="entry name" value="H_N_K_Ras_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000080">
        <property type="entry name" value="Ras-like GTPase Ras1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2071268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="190" mass="21409" checksum="5A6F91ECDA8B3C5C" modified="2022-05-25" version="1">MTEYKLVIVGGGGVGKSALTIQLIQNHFIDEYDPTIEDSYRKQVTIDDETCLLDILDTAGQEEYSAMRDQYMRTGQGFLCVYAITSRSSFDEIGSFREQILRVKDKDRVPMIVVGNKCDLESERQVTTGEGQDLAKSFGCPFLETSAKIRVNVEEAFYSLVREIRKDLKGDSKPEKNKKKRGLKSACSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPA1</accession>
    <name>A0A8J4PPA1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006971</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000347">
        <property type="protein sequence ID" value="KAF2071710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPA1"/>
    <dbReference type="OrthoDB" id="18975at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36337">
        <property type="entry name" value="OBSCURIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36337:SF1">
        <property type="entry name" value="OBSCURIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1114"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1228"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="68"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="364"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1230"/>
            <end position="1240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1253" mass="143862" checksum="03D6F9EADBE17A09" modified="2022-05-25" version="1">MNKMKSGTNYPNSSSTTDDHIQQTRYNTTANSTNTNTTTTTGTTSTTSNTTPTTSIDQSNNSTAASPNQHLLNNTLQNTHSNSSNNNNNNNNNVRKQKDELYWCWLLKEYIRLGHAKSVVRIIRIIDHLSPYLYFYKDLIYETQKSAITTTTTNEPSVPSTSISTINKTESEIIAAIQDENNISRNNINRRKDFLFHYQSRPIFDPKRTNFPYLYIPNLIRQRSDLFQYFNRSFSFNNILFALSNGTIDTLILQNFLPIPKSIPMIPFLLTLVYIKLCVSKGDALQSIMNSLMECKQKAHLLQSYDFPSIPIDQLLDSIMQGTLRFVDWNHDTYHLVSTEVFFNELFESFFLIGRESLLNREVNSSNNNSNNSSNTQLLNQSQQQLQQRNNQQQQQDKQQQKTSNILPSIPLKDEYRFKIIDIYLEKSSKMVYYHTRALAQKRGNIHSLTWTLENVNILHKCIKYYLDVYLSQQQSNSYNQETLSILDCCMRLAFSWVAACSGLHPTPNNQKYIELESCWWNSHPQNLQNELQHWMITIKRILHQVVGQESDKLHVICLVWLSYTISLYDDDQHFDTLQQQQQNDQPKQPLQLEETPYQLYTKTITQHPKLLLEFSKSSLVITSLSFALIGVQTLDQSAELIQILLSLWTNNQQQPSLENACLIFKLLQNVLSTKFKKSEFSDHTELVMAIIAWMRCISDEVYSSFYSSNPNCRFICIFIASSLVNAVQKNKNIYSLIPLEEKNILISKVQEIFYTLINQHTFLSKSFKTGLATAYCLSSIEPSFDKQASQSILMISLDVLFDELLGIDNCLISWLDKPQEINYASIKKIFEHNKSPLFLALAPFIQTITRLTLHLQDNDNNKLVLLKIFDFTYSLNEKWVQFTKDSSSTSTTKYPKSYIKSTLDPTMRAISQTLSFIFTDISSILTAMDSVLGVEIFSQMSYTSNDDIPYINSAIKSLVKKVATSECAVIRLVDKMPVYFELPKDDPSGPCVSKVILYLSALDSLVVHVPHSLIIEHIVPNLFGYMEYPNSKLNMMTHTVMGKVLSIPNFSLNIKMIPMYLKLSLKSFPKYTKLSSFYNVLINIIESNSPTNPIVLYSIKTISQTIIEQMKNNNTNNINNNNNNNNQDKTKINNNNQNNNNNLNLNSTTKGLVKLLLSLFTYIDIQIIELVLNEISKVIYSAPHQKIRLDLCSDLLTVITGNIENNSKKNICVSWYLKLIKDIQKTGTNNNNNNNQQNPPSSPNLLIYNQNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ29</accession>
    <name>A0A8J4PQ29_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_006605</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000312">
        <property type="protein sequence ID" value="KAF2072085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ29"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF298">
        <property type="entry name" value="MIXED LINEAGE KINASE DOMAIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="315"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="106"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2072085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="654" mass="76257" checksum="4E0299C2F4083C15" modified="2022-05-25" version="1">MFLRKLFIKSDTGKFKRFGQQMIQDKQLSESNLKEWIKLFRIQDDKYIRMFRYYHIFNNMPISQEIFDFLITLIISGDFSISSSLSYHNTEYQEVSTILPRSSSKRKKLKQMASETSSDSEEEEEELNTSRGVDNISRKSSLILTSTNFTKYKLNTQFIGLAILYRIIFNQQNINYFISKQHLSSLGTLYQSYIQHLKTTDDQLLMNYISNNSSRLLSIFFKLLDQETLLPMFFNCNLIESISRLTLEKPNLSGKLIQQDLDMSDKFVEFLSKHYNQEPYHSIIESKPEWIDLMISYSAKQKVGLIHQELTEGMVEFGDSVYDSPLCTVYKGHFNGLEVAIKQFSVEGISFEWPLFFKEVAIICVTQHPKVIRVYGAHTIKTDRPFIVTEFCHRGNIQQSLANYKVETGNSPPVPLLVNMAVDAAQSMAFIHSKSIIHRDVKTANFLVNKDWNVKLIDFGISRVMQTQLNMTVIGTPVYMSEEVIRGEQYTQSADVYSFGIVLWEMFTQETPFKSLTDYQRVNFVLEGGRLEIPTTVPSKIAHFIQLCWARDPTVRPTFRKILDFFYSLLNPSPENHRMVPVVTRFNDSPLPRIIFSYLDKVSMSNYGLASREMRSCLHRTIHSNPSMNENYWTELLDYTLHKYYNQQHQLQIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ39</accession>
    <name>A0A8J4PQ39_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000421">
        <property type="protein sequence ID" value="KAF2071017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ39"/>
    <dbReference type="OrthoDB" id="21036at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="180"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="242"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="270"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="341"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="368"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="413"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="522" mass="57561" checksum="00B3D8E0CA1D1796" modified="2022-05-25" version="1">MDPIIQIMDSIKSLSNRSLIETLKMYEVINDRYTKEELLDKFQQKVFQETYHWIQKLDYIVNPLAQELKCSVDQLPEVFKQDLGSTLTKLSEDLIDKVFRCLKLGADEEPDVSSEDKVTRIEEELLLQGVFKLLYRLDEDVLRLYCTDLSIQYDSDKQKVANSIMNVMYDLEVDEPTPTPTTTTTTTVTSNGNGNSGNGNSSNGSGNSNTTSTATTTTSSTSAGNGAHIKKEEEIIKQEPITTTAATTSTTSTTPIKEEPKKVPKRAAKKAAASIPTPTPATTMKRRAAVSKSADTPEDSEDSKDSEDSSEDSSEEDEPTPKKTIQRKRRETVAATKEENSNGSSSNSTSTTLGKRVRRAVEPYSVPSAASSSTTTVTKSTTTTTVTKSTTTTNGSNKKATKQPQVSDSSSSNEEEEEKEEMDEQEEAGDQFYDNHTNNNNNNGDSIAVKGHYINGKFIAPSISFIKLGYTANDLRNNYNLPDLLEYCKLHQLSKTGNKTKIIKSILNHLEGKVPQVKKKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ60</accession>
    <name>A0A8J4PQ60_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006695</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000320">
        <property type="protein sequence ID" value="KAF2071982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ60"/>
    <dbReference type="OrthoDB" id="10250130at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052637">
        <property type="entry name" value="KLHDC3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46461">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46461:SF1">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="91"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="487" mass="55554" checksum="0C8FBCD0976B0A6F" modified="2022-05-25" version="1">MISKLFKSFSLKNLTNGNSYNNNNLNRSTSTPNSFSIILSNQDDCINNDPTDSWWGSNNNNNNNNNNDTSFTISSYYSPLPSFILFDQLGDEIKLYILSFLSIKDLFHFAQTSKSMMHYAFDKVLWVERCRNQNGWSHGNDLYSKLSSTNLISYYHDKSFFSNDMKFQSIDANGNIPSKRYKHSATVFKDEIIYIGGQETDSKRFCDIVYYNTKTNKFTMPKIKGSIPKFSRHTAQLVNDKIYVYGGYNGMGTYYDLAIFDPIKKRWENILNQDLKGDIPLSRSNHTSVVIGDMMYIIGGNRSNKDGSHLLMDDMYCFDTKNKTWTNIVNPKGQVPCGRGGHCTETIDGKIYLFGGGVWDPSYQWTNRFNDLHIYNPTTNEWSKPDVRGDIVPMTSTFSISFVYGSFFYLFGGGSIESNSVTNQIYAFDTFTNNWQKLNFSSFPPKPRDMSTASVINNNVFIFGGYAGGAISYLDKITFDFIPKLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ74</accession>
    <name>A0A8J4PQ74_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006519</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000305">
        <property type="protein sequence ID" value="KAF2072160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ74"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="76" mass="8008" checksum="5CD236253C1C0CDC" modified="2022-05-25" version="1">MIFKALSSISSPFKSMKSSSQTSMTSGSSSGNGQNGNSCYYIIAIGGPCIWVADNANPHKYYFGYPPATATTNTIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQG1</accession>
    <name>A0A8J4PQG1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_006511</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the recoverin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000304">
        <property type="protein sequence ID" value="KAF2072168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQG1"/>
    <dbReference type="OrthoDB" id="15980at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF178">
        <property type="entry name" value="NEUROCALCIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0519">Myristate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="56"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="92"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="151"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2072168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="207" mass="23783" checksum="D051006CF9CE810D" modified="2022-05-25" version="1">MGSGSSKQDFREIAQHSRLDYKDLENLLKTFKSISKNGKINKDALKKTIEQKFGGMDPTFSNVLFNLFDRNGDKDIDFQEFCLAYGYLVNRSHDDVVNVSFKCLDLNGDGMVSKGELRSVVMMNKRMEKYMRHDKKIPLDKIQLDPKETSEIYAKADQLFNMLDQNGDGNINTTEFQNIVNGNEAIQKVFTDLLVKDHSVDFFLDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQH3</accession>
    <name>A0A8J4PQH3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_006783</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000328">
        <property type="protein sequence ID" value="KAF2071904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQH3"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000098">
        <property type="entry name" value="Mitochondrial substrate carrier family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="4"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="69"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="106"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="178"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="270"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="366"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2071904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="467" mass="52178" checksum="809532E3D2EE636B" modified="2022-05-25" version="1">MDKEDKDFIKNLFVSLDTNGDGKLTREELEQGFLKLKIPPTHLDNLINQVDSNHDGLITFKEFEDFTVDNIIKLKTVFNEIDVDRSGKLDFHEIEETIKKLGLPTYSEQELARLFSRIDKNKDNLIDFNEWRELLVLLPSNNLSVILSYWKDAQFLDPGSDSGGFIPPPPTKAVHSLYNTFVYMGAGAAAGIVSRTSTAPLERVKIVYQINHGAPQSLMQTFKNVYADGGFRGLFRGNLANILKVSPESAIKFASFEAVKKLFAAHDSELTSAQRFISGAAAGVISHTSLFPLEVIRTRLSASHTGTYTGIIDCGLKTYRAENSFRPFYRGLVASIVSTIPHSGINMMVYETLKHRIIKMSDNPYPPAAYLMGCASISSVCGQLVGYPFHVIKARLITQGTPVNPEIYNGVIHGLKTIVKKEGFKGLYRGIVPNFMKSIPSHAVTFGVYEFFKKTFDINLKEKKHHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR30</accession>
    <name>A0A8J4PR30_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pleckstrin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_006610</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000313">
        <property type="protein sequence ID" value="KAF2072068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR30"/>
    <dbReference type="OrthoDB" id="245697at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00014">
        <property type="entry name" value="CH_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000003">
        <property type="entry name" value="Rho guanine nucleotide exchange factor 10 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051092">
        <property type="entry name" value="FYVE_RhoGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001331">
        <property type="entry name" value="GDS_CDC24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673">
        <property type="entry name" value="FACIOGENITAL DYSPLASIA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673:SF80">
        <property type="entry name" value="PLECKSTRIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00741">
        <property type="entry name" value="DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="3">
        <location>
            <begin position="7"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="2">
        <location>
            <begin position="614"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="417"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="473"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="986"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="337"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="417"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="485"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="519"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="989"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1025"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1049"/>
            <end position="1059"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2072068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1059" mass="118941" checksum="0C3E2C040F465054" modified="2022-05-25" version="1">MKSSSFLSKKRAAIKWIENVLECKIETNKDFIETLQDGVLICKVMQTISPRLMPRISIPDPNTPQRTILFKYSENVSFFIQACTDMGVPRHKRFALSDLVHSSNSSPSYTNMKRIIECLESVSKIANSDPQYEFSIEWPEIDQEEFSEEEIHQAEKLLAQFTIRESKRQEVIKKSQSNDVPSVEATKNQLFQHMAAQKQLYPDNSPQKVKPSSPSSSFNHHHPPLHPQPTTRPISHYYTIRPKSYSLSQINNNNNNNNNIPFSSSPPSSASPLSSSPNIFTPPASTTTTATASTTAPSSLSPISSPNFDDVICSPTTLSSPLLQPTTTVNDDHIVINNNNNNNNNNNDEGTSNNNAITAKEQPRPYSPTIELQKEPLSTPPTIQQKPTFKDILNKWKNPSYFSNDHRAVDLYNSNSSNSTIQPNRQRSFTMPHPISSSSSGSGNKMNENNNIDVYSNCNIKNNNNHYLSQKKEEEEKIATTTTTSPSSLNNSPKLDGVSNRNSLSSPIKLNPIFTAGANSTPTTTTTTTTTTNTTSSNTASVAPIPSNNSTSITSTTATVTPTTATPSKPTTPTRPITPSMEVIKERAAIKIQRATRKWLERNRQRVLARDAAYRERIVKEITETEEQYLDRMNYLKTVVLKDLRDAVNKGSPIISIEEIQTIFSEVEIIEAYNQKFLHDFKTRTANFKKDTMIGDIFIKFSQFLKIYSQYSRNYSEAMNALNECKKQSKFKSYLEKVKHDRPEIGLRGLEDYLIRPVQRIPRYSLLLKDMISHTWKSHPDYAQLEEAFQKMNSVAEYMNEKKKEAENMMKLAEIQEKLDGESSAVLAKSFRKFVKEGDFVEVLTKGKKAPIVIYLFNDSLAITRPTKSSGSSFFSKQKTIRLQFEQIFLLHQLTMKEYESVCGVANSLKLQSKKDSQISIVVATHDRDTLDEWIKAINAEKAEDEKNVKEQNDRISSSINEKVIATKQKIEEKFTRVSGQYSASDVESIGGRSDSSSTGSLSNSTDSTGESGGVPADLKSGKMSLRERRVRLAQESKNKRLSGHMAAMNSSTDLNDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRH8</accession>
    <name>A0A8J4PRH8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000356">
        <property type="protein sequence ID" value="KAF2071630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRH8"/>
    <dbReference type="OrthoDB" id="21103at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="649"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="654"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="684"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="694"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="717"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="734"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="785"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="806"/>
            <end position="815"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="815" mass="92603" checksum="31510C00553444CE" modified="2022-05-25" version="1">MNNEKSNNDSIKSILSSFGPSGKKIAPMISKKPAAGPNSISIPSTLSFPKRTASALHPDKAAPAPPPPPPAEAKKKAATKAPGESKKAKELKRRFEERATKNGIQVSKISTDPKNKKTKYEDISLDDLLKDKRDQKSLFEDIDLDYVKPSERKDSKKEKKTVFDSNVLDALNETFDQDQIKNIQSSISSQHQDLLLNEYPNQQCIKNDIPKLSPMTIKDIDKQTEKHPIFNLLSNIQDDEAGVKAFLMNSSMVLFNNGKSPLPKSMIEFFYSFVCHGFDDNLSYKAFEFLRLYCDKYPLLISFQQFITTFKEYKIFNLLHCFNDDSFSAAGTNSLSKKKVVPTTPFPLMKFNSILSLIRKCIKSFSTKELKDMSILFILFSIDPKFLDKNPTFHKNIDKRINSSKIFAETFVDRSVNLSVKESIFSGLGELLAEYTLKTDYDISKLYELFEDLDNTLESTKTKMSLRTVISQNIPFNCSKIPVQRCYSLYSISKILEKKDIISIISNKNIADASYNILEPMVSRIRNNSIDIHNMINIVQLFDICTNNREELNRQTYEKIDKLLTVWNSKIKESKDFDFTRTRIKDLLILIQSKIVGISQTLRDYSTKNFFSPVKPKVSNSIKTEEELGKLADDESDDEGDKMDIDVAKKPEINNDNNNNNNNSSSDNGNKEKNNEITNNKKQSKNEDGKENKTTNIENRNNESTTTTEINSEDTNDNEKKTEDSNKNEETNTSKENANNNGDSNNSQSTKDNENNITTETKSENNEKPNNGEINGKNEKNEFTSSNENINNGNSNNNENNNNKNHSTEELKDTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRK2</accession>
    <name>A0A8J4PRK2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">eRF1/Pelota-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_006909</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic release factor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000339">
        <property type="protein sequence ID" value="KAF2071781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRK2"/>
    <dbReference type="OrthoDB" id="10254527at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.60:FF:000001">
        <property type="entry name" value="Eukaryotic peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.960.10:FF:000001">
        <property type="entry name" value="Eukaryotic peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000006">
        <property type="entry name" value="Peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.960.10">
        <property type="entry name" value="eRF1 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024049">
        <property type="entry name" value="eRF1_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004403">
        <property type="entry name" value="Peptide_chain-rel_eRF1/aRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03676">
        <property type="entry name" value="aRF1_eRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10113">
        <property type="entry name" value="PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03463">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55481">
        <property type="entry name" value="N-terminal domain of eukaryotic peptide chain release factor subunit 1, ERF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="5">
        <location>
            <begin position="5"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2071781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="440" mass="49344" checksum="90DAC065898DF3EF" modified="2022-05-25" version="1">MADEQAGADKQVEQWKIKKLIKNLEAARGNGTSMISLIIRPGDQIAKVSKMLAEEYGTASNIKSRVNRLSVLGAITSAQQRLKLYNRVPENGLVIYCGTILTDEGKERPVKIDIEPFKPINTSLYLCDNKFHTAPLGELLESDEKFGFIVVDGNGALFGVLCGSTRTVLHRITVDLPKKHGRGGQSALRFARLRLEKRHNYVRKVSELATQFYITNDKPNVSGLVLAGSADFKTELSTSDMFDQRLREKILKVVDVSYGGDNGFNQAIELSTEVLSNVKFIQEKKLISQFFEEIAQDTGKYCFGVQDTLKALDLGAAHTLIVWENLEYTRYQMKTPQGEDKVVILHKDQEKNVAHFKCPETGADNEIIEEMPLVEWFANNYKNFGATLEFVTNRSQEGSQFCKGFGGLGGLLRYQVDFATLNDYDNIGEENEYDDSDSDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRP4</accession>
    <name>A0A8J4PRP4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RGS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006557</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000309">
        <property type="protein sequence ID" value="KAF2072112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRP4"/>
    <dbReference type="OrthoDB" id="196547at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07440">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845">
        <property type="entry name" value="REGULATOR OF G PROTEIN SIGNALING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845:SF273">
        <property type="entry name" value="RGS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RGS" evidence="2">
        <location>
            <begin position="7"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="203" mass="22991" checksum="E6E85E53F699F0DD" modified="2022-05-25" version="1">MIYPNDVMNEVLRDEVGCGLFKSYLKTIYCSETLQFWLEIENFKANAPPATPTLSGPILAFSSAPIESSPSSSSSSPSSSSKNRPSSPSGKYLSQEELRVSAQNVFDKYLENGSEFELNIDAFSRELVRQALNQEIDSSLFDDVQKVVYESLWMDCFPPFTHTAAYHKYKQDTGKCHLPEFLRISFKKKSPKKDDIRMKTVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS02</accession>
    <name>A0A8J4PS02_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007353</name>
        <name evidence="2" type="ORF">CYY_007360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000387">
        <property type="protein sequence ID" value="KAF2071322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AJWJ01000387">
        <property type="protein sequence ID" value="KAF2071329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS02"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="66" mass="6771" checksum="7C27BB8640824A74" modified="2022-05-25" version="1">MTLIKSITSLGSVQNGSISKVSISSSSSSFGSLNQMGANQNSSLADLDICVKLLNLLGISIKAKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS58</accession>
    <name>A0A8J4PS58_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_007241</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Neuron projection</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PTEN phosphatase protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000374">
        <property type="protein sequence ID" value="KAF2071449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS58"/>
    <dbReference type="OrthoDB" id="16692at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016314">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08368">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd14509">
        <property type="entry name" value="PTP_PTEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051281">
        <property type="entry name" value="Dual-spec_lipid-protein_phosph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045101">
        <property type="entry name" value="PTP_PTEN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014020">
        <property type="entry name" value="Tensin_C2-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029023">
        <property type="entry name" value="Tensin_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305:SF97">
        <property type="entry name" value="LIM-DOMAIN CONTAINING PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305">
        <property type="entry name" value="PHOSPHATASE WITH HOMOLOGY TO TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10409">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22785">
        <property type="entry name" value="Tc-R-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01326">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51182">
        <property type="entry name" value="C2_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51181">
        <property type="entry name" value="PPASE_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0440">LIM domain</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Phosphatase tensin-type" evidence="14">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="13">
        <location>
            <begin position="86"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="C2 tensin-type" evidence="15">
        <location>
            <begin position="157"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="12">
        <location>
            <begin position="366"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="12">
        <location>
            <begin position="432"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="12">
        <location>
            <begin position="561"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="12">
        <location>
            <begin position="644"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="12">
        <location>
            <begin position="704"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="338"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="796"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="936"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="522"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="338"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="807"/>
            <end position="832"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2071449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="961" mass="109072" checksum="8A0BBA55314531A6" modified="2022-05-25" version="1">MGYPSESELYRNQMKDVQRFFNTLHPDHYKVFNLCSERKYKHDRFNNNVSEYPFDDHNPPNLDTIGLFCKDVEDWLELDPENVAAIHCKAGKGRTGTMIACWLLYNKHCQTGSESMRVFATKRTHDSKGITIPSQMRYVRYFEALLQHGRPVQKVPDITKILSTIKMNALPNFNIGGGCTPYLGIVQQNKFVFYTKPLNLKKGTLEIEIYCGNILLSNDVKIQFFNKNSKGHMFSYCFHTAFIEGNTILIRKLEIDKAHKDNKHFPSNFSIELHFIDKETLDQEDVLINDQMNQTDLKSSSNTTTNAVPTTHYATGLAKLYGNNDQLLTTTTTATKDNDCDGGKKENDQDNQQQSTVQRKQSTRLFICPKCKLSINSSDVSVNDGNNNYHWKCLVCNNCQKSLANENDCLFQESGISETLCTSCGTKSGFFKTCHACNLVIKSSDFEEVGNLSYHTSCFYCTHCLLFLGDKDFTISDKKELKCSSCLHKPSIVQKEEIQEVEAEEDVKVEVEQLKVEIEEPKEEEEIEIENEIEDEIEIEIEKVDLTQLEINNQENNSCDIKCTKCKLEIGLRPLVLDIGIWHRECFVCQHCEIILDQKRYFLRDDVVLCSECDIKHILNKPVVVSNDGELVASSASMMAQSEIICNGCKLTIDDEEMMSALGSKWHLQCLHCCTCQQPITGLFGEHQGLIYCKDHFEEITGVRCEGCNQMIVGRYLKANGRQLHPHCFICFICTRVLQDGVFYEKAGEIICDRCRSTDIIKRRTQLQLQQSMIGGKRSLTASAVASSGSGSSACGTDSSSSLAPPHSNTLSRSLTTSQAHQTSLTGSISEGNSDEHHNQHSTISYVHNIRSSFRNSQRINLSDLKKNRTLHIYPSFHRQMKMNINNNNNNINNNNHNNNNSPSTSTFTQTSPKQFIVAYPSSKNHSKRREFLNHTSHSMNNNNSTGNDNNENIQEINKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSW0</accession>
    <name>A0A8J4PSW0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005474</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000219">
        <property type="protein sequence ID" value="KAF2073223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSW0"/>
    <dbReference type="OrthoDB" id="20796at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="204" mass="23030" checksum="CE9CAE54AA484699" modified="2022-05-25" version="1">MILITSYNSKSKKLCNYCSENIPCNLMVVREFTPNDKIVIPPLTKPDYSVPSGLPKRPMVTSESCSSIPPNNNSDLSIPVVNRKSPDLSKLNNNNNISEQHQPSTTVIYATGKRPSISKSMDSINLNQQKQEPTILQSNSEEFDNSSSSEEDDDEINTHHHNHHNNNRNEFENYHLISTPVEVHKEAPTKAFPQKSHEYVTSQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT92</accession>
    <name>A0A8J4PT92_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">2Fe-2S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_005786</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adrenodoxin/putidaredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000240">
        <property type="protein sequence ID" value="KAF2072891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT92"/>
    <dbReference type="OrthoDB" id="268593at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140647">
        <property type="term" value="P:P450-containing electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000013">
        <property type="entry name" value="Adrenodoxin, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001055">
        <property type="entry name" value="Adrenodoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018298">
        <property type="entry name" value="Adrenodoxin_Fe-S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426:SF65">
        <property type="entry name" value="FERREDOXIN-2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426">
        <property type="entry name" value="FERREDOXIN/ADRENODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00355">
        <property type="entry name" value="ADRENODOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00814">
        <property type="entry name" value="ADX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="11">
        <location>
            <begin position="45"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2072891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="160" mass="17808" checksum="FED1C08A13372519" modified="2022-05-25" version="1">MNIIKLKSIFTPSSLVTSYSRCGDKSLFVRYFSSKASNTDKKDLVTFNYINKDGSKSTVSAPVGENVLEVAHDNDIDLEGACECSVACSTCHVYVEPKYYNMLPEPTDEENDMLDLAFDLKENSRLGCQIVLTKELEGMTVTLPSASRNMFVDGYKPPKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTA8</accession>
    <name>A0A8J4PTA8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">THH1/TOM1/TOM3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000261">
        <property type="protein sequence ID" value="KAF2072635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTA8"/>
    <dbReference type="OrthoDB" id="17382at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="295" mass="33946" checksum="B6A90968694155F8" modified="2022-05-25" version="1">MDLKFDISHGILVVLYFGLFIFSLIKLLIQKKSVIRVLFHFFLLLGCAFRVTSFTLFILLNEDVISVDGSFLYLTASLPSFFFLTFYSAALVLSVDLFQISTKIKPVNIKTLSLIVLLANICLYVMVIILFVLDFEVFPDEVASLNAPTSVFQKTIQFIIVGIYLIASVSFFLFLYIVYYKNYETYFRDANVNVEYNQLKQKSNGVIFFSFVCFFLRSFFTCLTIFYPGASLWWKDLAYYMGLEFLPLFLLLFIYLKVAVTTPGLHTFHPYSQLHDENSKLTANSGYIARVYGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTG0</accession>
    <name>A0A8J4PTG0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009565</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000743">
        <property type="protein sequence ID" value="KAF2069116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTG0"/>
    <dbReference type="OrthoDB" id="659430at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="477" mass="53429" checksum="C5956B382E157327" modified="2022-05-25" version="1">MPPSITHLTLLLQTINTLQESDIPSSIQKIEFKSLAIETNPSVSFEIPKHIASRLVNGVFFIYSKSNNKITIPTDTTHLFWLDDHYINNDDILLPPSVHTLVLGKGFNSAILSLPPSITQMVFNGEFKQSLETICFPLGLKYLSFHSLNESIQEHHLPNGLTHLVIEYGGCPSNLPQSVHHLQFSECGAFSSVIPPQVRHLRINRINSKLHVPPTIKSLITSSHDRVYFDNIYPLVFSVLEKDSIEYNIQFSLPFGSDTNIHLHALDSNFNTFIKPYTLGNNIKSISFGDVFNQVIIKGSLPNSVEYLEFGKRFNQKLNKSILTTTLLTLVLGSDFDQDLTDALPSTLTELMLNTKSKPSFTSSSQFPPNLKKLALPCYDGVLDIVPTTINHLEFNRNIAKQKYVIFPIELVPPHITTLVLNDSMRIQSYNLIPENITSITLCDSITPGTKIPCTVESVVLPSSFNQPLDTIFQTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTR9</accession>
    <name>A0A8J4PTR9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005281</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000206">
        <property type="protein sequence ID" value="KAF2073402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTR9"/>
    <dbReference type="OrthoDB" id="14098at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="97" mass="11524" checksum="AA225690CAF7B727" modified="2022-05-25" version="1">MFSATSLTLKSLNAGRLMKPIKQTHTWRMGRKKRDSAIKETEDFLRKYNTCLSKKLPKPRNWTEEDVPVPLYKQDHVKLLEKYGYGNTIYKTLQKYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTV4</accession>
    <name>A0A8J4PTV4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AN1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_005395</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000214">
        <property type="protein sequence ID" value="KAF2073296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTV4"/>
    <dbReference type="OrthoDB" id="431929at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14677:SF41">
        <property type="entry name" value="AN1-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14677">
        <property type="entry name" value="ARSENITE INDUCUBLE RNA ASSOCIATED PROTEIN AIP-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="AN1-type" evidence="5">
        <location>
            <begin position="9"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="AN1-type" evidence="5">
        <location>
            <begin position="95"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2073296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="169" mass="19084" checksum="D7988B5741DA8DA1" modified="2022-05-25" version="1">MVDTEKDLDKVGVHCNLKDCHLLDFLPFTCDGCRKLYCVEHKDYAEHSCPSPPVIKGGCTVTCDKCDTILPVPFGKDDTFVLRTHISQGCKKKPSINQHKCTFEGCSKSESIKILCPQCKNNFCLKHRFERDHKCKAPKQNVVMPKPLITPHLANMLDALRNSKPIKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTZ3</accession>
    <name>A0A8J4PTZ3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_006181</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000273">
        <property type="protein sequence ID" value="KAF2072514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTZ3"/>
    <dbReference type="OrthoDB" id="128483at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34500:SF1">
        <property type="entry name" value="DDE TNP4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34500">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="4">
        <location>
            <begin position="72"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="234"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2072514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="269" mass="31479" checksum="E2E964E85ED81ECA" modified="2022-05-25" version="1">MTLDYLRYYTTFADRCRIFNTCYKTYSTHVWKVLAANIKLKFIHFENRINFKVPDATSIQNNGHLTLSIDTTFCPINVKDESFYSAKHSANGVKYEVGTNLIDGEVMWVNGPFKGRMADINIYRNNLKNVQIKDGERFVADIAYHGEKEKLLTPHKEKIMFVNGKKEIFMEPIKKLENSFIKHHRIINENLFSVIKRFSFLEKEWLSSIDRHSQAFMFVVNLFNISVTLNRCPPNENETDESLEDGKDTPPISYEEFKNLSFPNTKSYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU36</accession>
    <name>A0A8J4PU36_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase C1A papain C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_005046</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000192">
        <property type="protein sequence ID" value="KAF2073627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU36"/>
    <dbReference type="OrthoDB" id="190265at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000117">
        <property type="entry name" value="Probable papain cysteine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035240816" description="Peptidase C1A papain C-terminal domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="3">
        <location>
            <begin position="60"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2073627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="297" mass="32864" checksum="E7337515E3868D48" modified="2022-05-25" version="1">MKMIKSIFVLLCIVTIAWGVNININSNVGPNKTCYKKSETEFVPRIVSPQPKDYVNLGDIPNNWDWRNVSGRNFLTMNRNQHIPQYCGGCWAFASTSALSDRININRQGAWPEVNLAPQHLIDCDGGGTCDGGDPASAYEFMADNGMIDETCKPYRAKNGGQCTPVCTTCSPDGTCQKVTTYTNVTVTEIGKVSGTNDIKKEVYTRGPVACSIRATNKLEAYTGGIFKEFSLDVIPNHIVSIIGFGVENNVAYWIVRNSWGSYYGENGFFRIVQGDMFENLGIELDCVWAVPDISRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU44</accession>
    <name>A0A8J4PU44_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ZZ-type zinc finger-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_005528</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000222">
        <property type="protein sequence ID" value="KAF2073175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU44"/>
    <dbReference type="OrthoDB" id="18674at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01767">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000245">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46115">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22562">
        <property type="entry name" value="UBA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Thioredoxin" evidence="13">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="10">
        <location>
            <begin position="169"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="ZZ-type" evidence="12">
        <location>
            <begin position="260"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="UBX" evidence="11">
        <location>
            <begin position="434"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="105"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="231"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="336"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="136"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2073175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="515" mass="57312" checksum="9510EFAD78B07B51" modified="2022-05-25" version="1">MSRIKVLVDGQLNGELKAAGKKLVVVDFTATWCGPCQMIAPEFESLSKQHPDVVFLKVDVDQCKNTAQEHRVSSMPTFHFWLDNRKVDEFSGADRNKLKSTIERLKPDTTFSTPGQSLGGGRGNYQSFLDNLESKQNQQQEQYQKQMGSNNTSSNSSNNSTANKTRPTSFEPNPIMKGELIEMGFPENRAIKALYNVQNSSSQNAMDWIFENMDNPTIDDPLDVQVVAAPAAAPAPSSTTTTTATTVAKTPDNPHGIPTIHSALCDMCKQQIIGIRYKCKVCPNYDLCQACRDLKTHQADHEFAEHTEDIQNYQLTPEERALQKQRLDEKVQEIRKKKAEEEAKREIEREISRRESGKSNSAAVEKWKEDAAKREAEKIRKEKEEDAKAKAKIKAKIEQDRLERFGKKPATAGASAPVAKPAAVPPVVPASAPKEYTDSTIQIRLLDGSTIKGTFPVDAKLIDIHTFLSKNTSQSGKFTLSNTYPRKIYTHDELKSISLKDAGLVPNGTLLVSKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUE4</accession>
    <name>A0A8J4PUE4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_005979</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000256">
        <property type="protein sequence ID" value="KAF2072699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="273917at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043634">
        <property type="term" value="P:polyadenylation-dependent ncRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000051">
        <property type="entry name" value="DNA2/NAM7 helicase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002058">
        <property type="entry name" value="PAP_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045862">
        <property type="entry name" value="Trf4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092:SF31">
        <property type="entry name" value="DSCP-N DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092">
        <property type="entry name" value="POLY(A) RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03828">
        <property type="entry name" value="PAP_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="5">
        <location>
            <begin position="1100"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="domain" description="PAP-associated" evidence="4">
        <location>
            <begin position="1331"/>
            <end position="1367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="68"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="346"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="469"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="633"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="866"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="906"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="957"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1279"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="68"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="346"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="474"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="506"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="546"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="560"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="609"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="650"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="686"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="712"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="730"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="764"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="794"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="815"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="866"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="876"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="906"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="962"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1279"/>
            <end position="1310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2072699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1481" mass="164963" checksum="4FF3470D54FA83A9" modified="2022-05-25" version="1">MERDYKKQVHSWILSIGHEELENILTDCNKIECDKLRAMYTEWQFRDGYFFDESVDIPGIPSLDEFTNSITTPSNNSNSSSSASSQSTLSSTTSTTSTTTTTSTTPKKKNSKTKDNASQEDNNILKKFKIEFRLSNDKSIYHFENLQPFLPERQVEEEKRIVKIIRLCDTKTRLDTFTIDSNSITNTELVQLLTKISQGGFLQVPCHLIKDGSLKDWRCEPPSWFISKSFFSLGEFIASWFERLIWTRFWEDHKIDGRKDQSNKQLPTKPLFKLLDSRKQNLKEFWSKMTVENRKRIALSKMGNILAGEIQSLNEKPEKVDLDFMEKFGNLVEDFGKLKLKDTATTATPTTTSKPPIKAEIPTPTTKLSSSVTKLRIPIHSQDMNKHRVHSSTSSSLSESPENLNNISTVFEEYLVQNPVKLIEFLCFSPLERAGSTVDMVMRQMGHQLQHIYSESLFMDLIHSEERQIKKGKSSSSLSISSSSTISSDGGSGSSSVVKKKKKKSTSNSTPTGSGGQQSSSTTNTNANTTTSSTTNVDSMEKKSTNNNNNNNNNNNQSSLRKKRKKKPAAKQRNSGKSIDDKGGSDTESEVIQTRPRRHSLPMDIAPTIKTQTGIPYLSTTEDEDGVVLSEGELEDYPPKDGGNSDNKKNNSSSTTTATTNNNIQSNSINKSNSTSHRILPRVGVNNGNSNNNSNNNSISSSPKKLNISNSAPPTTMNTPTKSMGRPRAISTSGDSSSVNPKPIFGGWEDPDDYADDDHGANDNDSDDENSDDDDDEQDIVKKENTNISPRPNITTTPVITTSTSTPTPVPAPINPTSTPTPTNTSISTSTTTSTTKNTPANNTIQSQIKDHHSQSRLPFNFFTSVESQKSTATTPKSPSKSNSSNNNVNNSNVNSKRLFFENQSSTNNIKSNSNNNNNNNSSNSSNANSSNNNNNNRSLKTTKSTGNLTSVFVAESENHQEQQQQQQINQTQQANQIQPQVQNQPTIQTSQQQSSQPNITTFKKSIMIKSNSTSSLPTPHQQQIKKQENEDKYKDWRHNKFFNQIQIFKQLNPFSPLQAYLQPWDEPPLPYPHPTFSIKLHQEILDFVSEVGERTYPHVQNCHIVINLIKSVVKKLWPNADLDIYGSFMTGLWLPSSDIDIVVNYGKNMSIKPKNAQFLLKVLEKQIRTDLNNSILTMLCIPSAKIPVIKLVTKENIAVDISFRESPTSVHTGIAARDLVAENVKEVMGLYPLAIVLKWFLRERGLNNTYTGGLSSYCLVLMLISFLKNNEHCPKIKNNNNNNINGNHNNNSNNNSSSNTTNSSSTVNTQPTLKATPCNCQNSDQISGFNIGCALMRFLEYYGVNFNYQMTGISIEENTFTFPLTKDDPVGNLFLINPLGNLENNINNNSNDATTNNNLDNNNNQTPPTTVWYNHSPFSLVVEDPFVKGKNVAAGAFNFGRVKAAFQYAFATLSNTSMNELSFEKESILSKILMPSNSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUF5</accession>
    <name>A0A8J4PUF5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">CYY_005855</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Plastid</location>
            <location evidence="16">Chloroplast outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000244">
        <property type="protein sequence ID" value="KAF2072844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUF5"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF135">
        <property type="entry name" value="TRANSLOCASE OF CHLOROPLAST 120, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="10" id="KW-1002">Plastid outer membrane</keyword>
    <keyword evidence="12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="G" evidence="17">
        <location>
            <begin position="6"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="AIG1-type G" evidence="18">
        <location>
            <begin position="174"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF2072844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="452" mass="52316" checksum="28BEDB681A8FCAB4" modified="2022-05-25" version="1">MKIDTIVVGRTGAGKSTLCNVLMDNNYFEVGHGLSSTTKSFDEKCVEYQGTVFNFIDTPRDEKLCAQKTESLFSKYPALRKCRNILYIGNLSSNSDNSLVRTKILEHLRGPKRSVFFDPSFSPSVVQENETWIQINDIIQDNYRLEQEIFFKRETKKLFNWRPQIDSIENEKIILLVGRTGSGKSTLANIITQGNQFEESGSLKYENQFQNTLWSFNKKTYLVIETPGIGDGDMKIRDLLSTISEICYKLKNGIAHIFYLSNGRFTTEDQICFKILTEVIFNNENANLISLVRTNFPGFRNFDKCKIDTDLIQEEISFLDKKKLGYFRNKVIHINNLNQDEEPSMKSRKDSREKILTLISCFQDQNSNPKVLNEFLDKIEIFGDLKIKLKDSSWNSKILKNQLGIEDLNLEIQKNLSKILANDIKDKNKPLWKIILNFFNELVENYPFLSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUQ7</accession>
    <name>A0A8J4PUQ7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Natural resistance-associated macrophage protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_004863</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000180">
        <property type="protein sequence ID" value="KAF2073836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUQ7"/>
    <dbReference type="OrthoDB" id="409173at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015086">
        <property type="term" value="F:cadmium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005381">
        <property type="term" value="F:iron ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00221">
        <property type="entry name" value="NRAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01197">
        <property type="entry name" value="nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037982">
        <property type="entry name" value="Nramp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706:SF33">
        <property type="entry name" value="NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00447">
        <property type="entry name" value="NATRESASSCMP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="281"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="441"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="465"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2073836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="519" mass="57847" checksum="A5167153D2778186" modified="2022-05-25" version="1">MSPRTDDSIIIDSANTASTINTDNVDIENLEGQDQKYLVDIPDLDKPEPAWGINFKRLWAFTGPGFLMSIAYLDPGNLESDIQAGAMGGYQLLWVLLWSTAIGLWLQLLASKLGVVTGKHLAEHCRDQYPTGPKIGLWLMTELAIIGSDIQEVIGTAIALNILSNGHIPLWAGVLITAADTFTFLFLEKYGVRKLEFFFCSLIAVMVIAFGIEYGISKPDQVEVIKGIFIPRVSHSNLSQAVGILGAVVMPHNIYLHSALVQSRRIDRKSDFQIKIANKYFSIESAVALFISFIINLLVVSVFAKGFFGSVDSIGLSTAADFLMDKYGKVAKYIWAIGLLAAGQCSTMTGTYSGQFVMEGFLKLTIAPWKRLLITRLTAIVPAIIVALVSTQHLDALDEWLNVLQSIQLPFAVLPVLYFTNDPEIMGKFKNHWANNIFVRFLSLVIITINVYLIVLFAMEESTANWMIALLAIGFFFYFSFCIYLFIGSKKFENVFLFFSKVRNFFRSGDQKYSTISNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUT4</accession>
    <name>A0A8J4PUT4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-synthesizing GTPase</fullName>
            <ecNumber evidence="2">3.6.5.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_008684</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000559">
        <property type="protein sequence ID" value="KAF2070001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUT4"/>
    <dbReference type="OrthoDB" id="1045173at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01888">
        <property type="entry name" value="eIF2_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03688">
        <property type="entry name" value="eIF2_gamma_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15490">
        <property type="entry name" value="eIF2_gamma_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000009">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000011">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000065">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050543">
        <property type="entry name" value="eIF2G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015256">
        <property type="entry name" value="eIF2g_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044127">
        <property type="entry name" value="eIF2g_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044128">
        <property type="entry name" value="eIF2g_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003077">
        <property type="entry name" value="PRK04000.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09173">
        <property type="entry name" value="eIF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="9">
        <location>
            <begin position="34"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2070001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="461" mass="50270" checksum="0D195ECFA1818DB7" modified="2022-05-25" version="1">MASVKAKPNLLPQDIATLNVNELNPLTPYVISRQATINIGTIGHVAHGKSTVVKAISGVQTVRFTSEFKRNITIKLGYANAKIFKCDNQECPRPGCYKSYRSNTEDSPNCERPGCGGKMHLLRHVSFVDCPGHDVLMATMLNGAAVMDAALLLIAGNESCPQPQTSEHIAAIEIMNLKNIIILQNKIDLVKEAAAQEQYGQILKFIQGTIAEGCPIIPISAQMKYNIDVICEYIVKKIPIPPRDFTSDPRMIVIRSFDVNKPGSRVDELKGGVAGGSILKGVLKIGDEIEVRPGVISKELDGKMKCSPIFSKIVSLFAEENELQYAVPGGLIGVGTKIDPTLCRADRLVGQVLGSVGKLPEIFTTLEVNFFLLRRLLGVKSEGDKQNKVKKLSKDDTLMVNIGSTSTGCRVIAVKHDLAKLALLTPVCTQEGEKIALSRRVDKNWRLIGWGEIQRGMSEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVE7</accession>
    <name>A0A8J4PVE7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005104</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000195">
        <property type="protein sequence ID" value="KAF2073587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVE7"/>
    <dbReference type="OrthoDB" id="22729at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="222" mass="25223" checksum="1BC4822201716B48" modified="2022-05-25" version="1">MGAVQSTQLDLRQDPSSMINDPRILESAKAQADEAVRNGVDANKIGQKQLRGTGLLVVLNLLKNILGNALPGPTPGRRLIIEVQDDSVGSLFVNINGVWKYMFMINGKDHQYNNFPIRVNTELQFLFLVNDLAIRWNYKTYNFAQTIIHIDKSYKRGFYCSVYGQSILGYDDYTFHEDDDRIVKGGNYPATRTAIFQLQSEPASKYTYDWSINSNTIVTIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVJ9</accession>
    <name>A0A8J4PVJ9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004258</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000147">
        <property type="protein sequence ID" value="KAF2074440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVJ9"/>
    <dbReference type="OrthoDB" id="20813at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="220" mass="24498" checksum="C82A092BAB7B9DE8" modified="2022-05-25" version="1">MYIFAEEIYSLFISDNSRYEINIYGKQKDRLKETLKNTFILLDTPESSLSTLSTPITSSTINSIGVTSTNSQSETFPKRKKKLRFRNLISKSSLSNIFTDNNGNIGESTLNRTTSSINLQEKSLSESNLSSSISSSSDDSFVVIFGTSAATATSNKKNNRGSTGSDSSSSDGEFEEFDRKNIQFGFSKCCNLFNDIQEELEQMMIENSLGEFLKSKTFKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW56</accession>
    <name>A0A8J4PW56_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GS catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_004845</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the glutamine synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000179">
        <property type="protein sequence ID" value="KAF2073849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW56"/>
    <dbReference type="OrthoDB" id="77835at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43785">
        <property type="entry name" value="GAMMA-GLUTAMYLPUTRESCINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43785:SF2">
        <property type="entry name" value="TYPE-1 GLUTAMINE SYNTHETASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS catalytic" evidence="4">
        <location>
            <begin position="159"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2073849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="504" mass="56444" checksum="C341E3DCE15F4CF7" modified="2022-05-25" version="1">MINNTIDSSEDVDNAFLKTRFLDIPQRLEVLKKIENDKRLKFIRVCWVDVANKIRCKSVNTRWLLEHRDNFFNVTVTNACMSFKAFEDAIVQEALPPNSGFGEVFLVPDWDSFTRHPSNPAYAQMFGSFYTVPKVETNAQGQSTTTHQRPLLAPWNLCPRNALKSAIDRLFANGQFTAKGSFEEEFYLIPREKNLQPTVNDLVWRDNSTFASCYSLDVYSDVLEKITDALELQGVPVEQLIKESGPGQFEITVPYTDILIACDRHEIIRQTVHSVAYECGFIASFIPRIYDNAAGNGCHAHLSLWKDGKNIIPDVSNPNQSGISNVGCKFIAGVIGHAKALTGLFNTTKNSFTRLKPNCWSGSNITWGLDNKEAFIRVPTSPMSAIKGNTNFEIKTLDHTANVYLAMAAVIHSGLDGIVNDVPIPLHTSLNPCTLSQEERTKLGIDVLPSNLITALEYLKKDQELVKNLGEDLVRAYTSVKLAEDKCLSSLTDEEIKLKLLELY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWC8</accession>
    <name>A0A8J4PWC8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004763</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000174">
        <property type="protein sequence ID" value="KAF2073939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWC8"/>
    <dbReference type="OrthoDB" id="18757at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053369">
        <property type="entry name" value="SrfA-induced_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32256">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32256:SF18">
        <property type="entry name" value="SRFA-INDUCED GENE K PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035230639" description="EGF-like domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="421" mass="46474" checksum="64D6C2807706D8A9" modified="2022-05-25" version="1">MKQKLSSCCIPTLLIGLSVTLVLASQCSNYYPFSSEAGDLNIYPGIGLDESLNQIYVIGKYNNSLNSPFGYPYVFSISTNSSNGNLTKQYPIKGEIPEGPGFTEYDRLYSYLEKYKYMYLRVNQRGSPQIGIYNPTTETFQPVWATRNIPLVIAFNQESTGIVFSDFGIYKLDKIPTTRDDQLDRVLLYQKQITNGISVQGTVIYISTMEGQFYRGTTLCRNCTQSNLIPLFQDPVAKSITGFLTSSSHMFYSSSLGIYSVPLNGSIDQRKTLVSEPVVSMVINKAGTIIYFTTSDAKIKSVNTTDGTITILYTNQKPVGQCECAVGFSLASDGSCTQCNGTTLWTSQGYPFCTKTIDGNKPEVCFQAWQCGYEPFSICDGKCTCLYGFYGPNCDQCDGQVAWSNGYPSCVPNPTISPLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWM3</accession>
    <name>A0A8J4PWM3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">D-alanine--D-alanine ligase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003277</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000101">
        <property type="protein sequence ID" value="KAF2075398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWM3"/>
    <dbReference type="OrthoDB" id="2017037at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008716">
        <property type="term" value="F:D-alanine-D-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011095">
        <property type="entry name" value="Dala_Dala_lig_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23132">
        <property type="entry name" value="D-ALANINE--D-ALANINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23132:SF23">
        <property type="entry name" value="D-ALANINE--D-ALANINE LIGASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07478">
        <property type="entry name" value="Dala_Dala_lig_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-alanine--D-alanine ligase C-terminal" evidence="1">
        <location>
            <begin position="140"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="335" mass="38407" checksum="3C9FF0EDD1DB8DD8" modified="2022-05-25" version="1">MISVIIEPSYDTRELIKTLNNLQLDYQIVLGPTDDYFNNINKTLQQAIKAPSGKPIRAIINLYEECNARGQMLADLLYNQPIPCTSPNASLYDITRYDLKSTCLKTNSKTPLYFFSEGMDSVDSIKLNLKGLMKKGVTNVFIKPGRGFNSIGIDKESCQPLMFQDESSFDTIIHKIEYVAQQFGSALVEEYIDGKEISILILPPLELGQQPLVLKPVEFCFKKALLDQNFITNEWKLNYEIEYWYEENAYDQEGCLEAMNMVKDVYQSFNFNTYVRFDIRIRKTSNNIVECFLLDVNTCCSVINYSTAVTILKANGKDNNWFVDYIIKSATNLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWU2</accession>
    <name>A0A8J4PWU2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphatidate cytidylyltransferase, mitochondrial</fullName>
            <ecNumber evidence="6">2.7.7.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">CDP-diacylglycerol synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">CYY_003576</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TAM41 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.41" evidence="6"/>
    <dbReference type="EMBL" id="AJWJ01000113">
        <property type="protein sequence ID" value="KAF2075141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWU2"/>
    <dbReference type="OrthoDB" id="341477at2759"/>
    <dbReference type="UniPathway" id="UPA00557">
        <property type="reaction ID" value="UER00614"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004605">
        <property type="term" value="F:phosphatidate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016024">
        <property type="term" value="P:CDP-diacylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015222">
        <property type="entry name" value="Tam41"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13619">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13619:SF0">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09139">
        <property type="entry name" value="Tam41_Mmp37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028840">
        <property type="entry name" value="Mmp37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="17" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00029893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF2075141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="392" mass="45029" checksum="656858A6DAC473AC" modified="2022-05-25" version="1">MKTNQRFLKSILGGLILNARFYSTTTGTATTKVNKDSLDKVNQLIKLFPKVKYGFAYGSGVIPQRGYQQQQQQQQNDTIVNTNNKPSPMIDMIFAVEEPIEWHNENIKRNPSHYSFLSYAGPSVVAKVQQIGGAKVYFNTLLNHNGIKYKYGIIDYKDLVDDLTHWKTLYVSGRMHKPILELPIVNESVSKEINLLNKTNNLSYCIAAALLMLPETFTEYEFYHKICSISYNGDIRMKGGENPNKIHNLIVDNIDSFRELYYPIIEDHFSNYIEVLLLNNQQQQEQLQPFSKSIIMDNINNFKLYSKKQSTRDVGLTMMFPKEIKSELLNKMRKNMKLNNGQMLTNEQDVSTIIHNIVSSSSFYQSAKGVLTAGIMKSIEYIRLKLKSSKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWW9</accession>
    <name>A0A8J4PWW9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Poly [ADP-ribose] polymerase</fullName>
            <shortName evidence="1">PARP</shortName>
            <ecNumber evidence="1">2.4.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_003952</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.-" evidence="1"/>
    <dbReference type="EMBL" id="AJWJ01000132">
        <property type="protein sequence ID" value="KAF2074720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWW9"/>
    <dbReference type="OrthoDB" id="10256774at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039253">
        <property type="entry name" value="APLF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019406">
        <property type="entry name" value="APLF_PBZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004170">
        <property type="entry name" value="WWE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037197">
        <property type="entry name" value="WWE_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21315">
        <property type="entry name" value="APRATAXIN AND PNK-LIKE FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21315:SF3">
        <property type="entry name" value="PARP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02825">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10283">
        <property type="entry name" value="zf-CCHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117839">
        <property type="entry name" value="WWE domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50918">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="1" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="WWE" evidence="4">
        <location>
            <begin position="165"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="PARP catalytic" evidence="5">
        <location>
            <begin position="402"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="3">
        <location>
            <begin position="598"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="707"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="712"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU362114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2074720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="754" mass="82998" checksum="8FD448610DF586B9" modified="2022-05-25" version="1">MPPKRKAVTLDEPVVASTVTTTTTTVSSSTTISTSTTTPPPPIQSDGNDNSDNNDSENESDQDTPIATTVTTKVSGPKSKKSLNLDVIVCKEGNYLPKEIGLLVSWKHNDNDFDPALLPTIESEYQSFLSNPSPSSKVIISPTISVDFGKNTMIDLANNSSDGSNTTEVLKRCKYIPLWVWEKRRSEFTSFPLDVSDKIDIAFVAGKSKVGIDPERYINLSTYEQARFDNANKTRKVKRIFFNTTEISNPAWYFKDSILGWQELPDSVNIEARYQSILKSSDELNIKLTNGDIFSFFTFTITKDKTNPPVEIVVKRDVGNIAPLKKTFSQLSEEEIKKLVNSVLSGDKSIDSIQPNIAAAITGATTTTSTTTSKSSDSSLYLPGEEDIIKSDKPMKYVFYPSRHFDSSAADFHFRTAESQFYRLLKSSSSSYKVTKVEYVVNPKLLKLFNKKKEEFEKLKYSDCNPVFGFHGTDSKNIAPICTNNFSVPGTNGVKNKTDSGWYGAGIYFSEYPEYSIGYISDCNKILLCKVLLGKSFKCNGLIVGQKCQSGYTSHLSPDEKELVIFHPDQILPCYVVHYDGGKVKANSTAHVAAQAQPSSNLLSGLKIATSGTGFNLSAANIKTLVAKHGGASAASINAAGITHLITNATEVANNSKITALESTNTHIVNEEWLYDSITAGKIKNTIEYSFYLRQTNTRGAIKPIGLNEDGALPDDDDEQVDTTPPKPQCKYGAQCYRKNADHFKQFSHPFLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXA1</accession>
    <name>A0A8J4PXA1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pesticidal crystal protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004275</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000148">
        <property type="protein sequence ID" value="KAF2074419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXA1"/>
    <dbReference type="OrthoDB" id="23795at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001907">
        <property type="term" value="P:symbiont-mediated killing of host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.10">
        <property type="entry name" value="Pesticidal crystal protein, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005639">
        <property type="entry name" value="Pest_crys_dom_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036716">
        <property type="entry name" value="Pest_crys_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35598">
        <property type="entry name" value="ENDOTOXIN_N DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35598:SF2">
        <property type="entry name" value="PESTICIDAL CRYSTAL PROTEIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03945">
        <property type="entry name" value="Endotoxin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56849">
        <property type="entry name" value="delta-Endotoxin (insectocide), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pesticidal crystal protein" evidence="1">
        <location>
            <begin position="43"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="481" mass="55499" checksum="F42D04C0DA7A85A4" modified="2022-05-25" version="1">MSEPDLTVIEKFCPLAAVGRKVLGDEYKLDSDDYMEIIKGTIDCIPVVGGLVNSVFSIFWKKAFPVEDPYLRKADFEKRMKKLITDMENKMDKKIDDEILKFCESKFQSLCSAGFGFAEIELIYDSEKQEGKPTSDSVKMYMAANHVSFRNLLLECLIFFSREDKIDLLCKLYLQTVVMYIAFLRDTHFHGLDWGLPEKTINGFGNNKSMASKTNETIELAHRYYSKFFKKLNTQTEDELLEARMMNAGHHGMRIEKKFKCDYRPLLAQMTYGDLTRYNLNQIWLQLTNEEGKLKEKKSGNEEDFELEMDLESRMDFRSYQYRLPVSNRIYIADFSKMNVMRGAFASTEEGRNSVNSVTGTIGMSSRDSNGLYFKCFYFTKPKNLTFRVLANIDSKAQIKMVIREIPEGEYAEDPDTSRDVATKSHCENVAYKKLFESSSGPIAEFVSKQYKFEKPGSVYFEAEVVGGNTRNLYTLEIIDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXD5</accession>
    <name>A0A8J4PXD5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004199</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000144">
        <property type="protein sequence ID" value="KAF2074494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXD5"/>
    <dbReference type="OrthoDB" id="19469at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398:SF1">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31398">
        <property type="entry name" value="MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="431"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="484"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="394"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="487"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="516" mass="59140" checksum="0B56FFD702F1B14F" modified="2022-05-25" version="1">MNLVNVIDKSTRFKNFCKKNSKYFLYLDIGATKVEDEPYRHRSKIGVLVSLVIFVILLFYIVVTISQFSKGVPTIIYSSLVASRYLPFPTPPLGILLRDKNNQETFDSRYYSVRYNYITIRNQDALPRVKVPLATVQCTFMDNENPQTGTQSIFTENAHCPNETVMMSGTYEMSTYNYLEIELRRCICAVPNDPTCTCASDAEFVENFFQGSVSLLVTEQIPTTTPLLNKISLIPENPDLSAPPKQVSNIKSMFWKSITPFYYKVDVFIRKQTIFNGPRYIFDYHYNDFYYVGKLDPKPEDYDSKNTTLFKAYIRLDSSDEQEIRQSPQVLQLIGSWGALYSFFALTFIRGVRVYNRKLYNLTTDINNAIKNLLHRTPSQVKFDRERDKEFDINNNNVNSSPPTTTIGASSSPTILVGDQSPVQKAYKIRSDSDENIPVVENNNNNNSQRNSREMDVFHRSKNLLLHSSAQSLTHSNLDSSWELPVNNNNNNNNNISLSSMSDLDQSQDKNNSPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXF0</accession>
    <name>A0A8J4PXF0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_004882</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000181">
        <property type="protein sequence ID" value="KAF2073824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXF0"/>
    <dbReference type="OrthoDB" id="412680at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF281">
        <property type="entry name" value="PAPILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035258180" description="Peptidase A1 domain-containing protein" evidence="4">
        <location>
            <begin position="22"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="5">
        <location>
            <begin position="48"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="420"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="433"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2073824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="717" mass="80523" checksum="FCCF71B943D3108B" modified="2022-05-25" version="1">MIFYKLFIFLTILFYFQNVQGSYVTSQIKAFPLKEVISVGNACPYKSYGLPVTIQNTTYNLLLDTRNIITTINAKDCKHGCIPADQYKPSPNSILERSRIFGYYVDDNAWGGELMSDKLILDTSIYVSYNLKFVSLKTQSKPISNPVCDGSSFLTSGLVGLGYSNDQNSFLRQYATKNFIDKIYSIVLCENNPRLWFGGFDSSILTSPMALYNLTSKSFGLEITDTTTPFIHLPPSLYQQTLQILGRSVPFTRYFSSRFFSEKKCILNPFNNMTNDEINAYLPPLRFSYRSTILGSEIVLVATSSYLIFDGDMVCPGIMETLEYNTIFGIPLFKEYLVMFDQENNKLGIGKATCKSTAWVPSSWTKCALTAIGCPIQKRTIQCQSEKGDILPEFRCSGLLKPPVTKDCIIRIKKTLKPTPKPTKRALGDDDSLPSSDSIISSSINETSTQPDPNTTPPVIRNLDSEIDIESEELVDSADFEDISRVKLSQCAEDSLTGTWKIVGNWSECDQICGTGAQTRKVYCIGKYGQKVKEAYCTAQKPPMKRICKQKKCVQDYVWYASAWSGCSNQCGGGTQSRQVYCVDSTIYKTVDNAQCLGPKPLPVRDCIGQPFCSKYKWDWKPCKPFCPEFTTISCYNMSTTEVVSDHYCQHLPNLFFPKACSCFVPPISSNETSNKLQEHSTEGSSSSSFSMPSRYYLDEKIITTMFLITILINILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXZ7</accession>
    <name>A0A8J4PXZ7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box/LRR-repeat protein 15-like leucin rich repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001640</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000040">
        <property type="protein sequence ID" value="KAF2077073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXZ7"/>
    <dbReference type="OrthoDB" id="18114at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box/LRR-repeat protein 15-like leucin rich repeat" evidence="2">
        <location>
            <begin position="267"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="F-box/LRR-repeat protein 15-like leucin rich repeat" evidence="2">
        <location>
            <begin position="479"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="F-box/LRR-repeat protein 15-like leucin rich repeat" evidence="2">
        <location>
            <begin position="666"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="133"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1059" mass="117728" checksum="587C27BC1C206DB3" modified="2022-05-25" version="1">MAHGEDDIIGDEDFTTSPEQQHRQPAAPQQQQRNISSLDEDDELPFSNGDNIVIGEVHDINDPQPYDPRNALYPPLNFPPQPQGHQFPSNISAPPNPYTPHQQPQQQQPPQQGQGQQQPPQQGQQPINPSHAPQSIPLQQQSIPSISSYPPNTILPSNPSQSPQSSQMPPMRDNNTGNLPMMFQPPPYQISSPYENVNIVYQNPYNQSPPPLSNIPIDADLMPNYTPDHLLDEPSSFGIGSIVQQELLSLKDKLINNLYIKPQIVNQIDDESLNSISECKGLLYLNLSSCNNFSKVVFNKAIGKLPNLKAINLSNCSQIVDDNIKTLAKSCKNLEEVHLNGCVQITDESISFLVEKCTKIKILSLSKCEKLTDKSIVSISNKLNGLTSLCINHIKYITQSSLPTLKKFNMKSFYAYNTLLTDPVVFETVPKWGSHLEVLNLGKCTNLVDNSISAIAFNCPNIKKLFLQSCKGITGLSINLIAQKCTQLNTLRVDYCTNLMDDAVVSLENLKCLKILNLSGLLKINELSLIRVLPRVPELEELYLYDCPRFSDLTVNQLCKSNPNLKVIRIDGTFFPTDESLCALSQACTALRTVNLSGLLKITDRTLFAFGNYQKYLQKLYLNGCKFIGNDSLYCITNNLFALEALRLDNSFQFSEDSLNSLGKLHNLKTLNISGCTHATNRTADIISFSCKQLTHLYLWNLPQLTSNCLPSLLINLPRLKLLRVDGCINIVMNGAISFPQKLCLEVFNCSETGIGYQTINSIASNCWLKELYCWGCENVTDDGLKELANHASNLSVLRIDRCKNISDKGARLLVQKTPLLSILNISNTNCGEDTINAVATYCKLLKKLYCFHCPKVVDRNIGAIAFQCQFINTINLSKCTNISDTGVIELSKCKFLKKVNLSYCSKITNASIIKLSLGCPMLKEILLKECVNVGEIGILSISTYCKRLESLDVSMCPNVTELAIVGIGRECLLLNSLEAMGISGKGNIDNGVIEVAVRSNINLKNLNLSNTHTSDKSIQMLVRMCPSLRTLNLVKTDISDTIIPLIYENCRLLKDFKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYK2</accession>
    <name>A0A8J4PYK2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_004236</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="EMBL" id="AJWJ01000146">
        <property type="protein sequence ID" value="KAF2074454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYK2"/>
    <dbReference type="OrthoDB" id="4096362at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF127">
        <property type="entry name" value="ZDHHC-TYPE PALMITOYLTRANSFERASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="462"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="414"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="276"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="352"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="15"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="291"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="360"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="373"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2074454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="529" mass="60618" checksum="BC0F8A4C84E7473C" modified="2022-05-25" version="1">MKKKTKLVGASTGDNNNKGVSNGSNNSNDNKNNNNNGNHKNKKKGNKKKTNKPSFSKIYFNGNFITGPDQLLFYIAMVLIVAPQLIYAIVICPTFEQKEIFSIITYVLLFLNIFYFLKAAFTDPGIIPRFKALQTEEGQDVVVLDIISEHTLRSLIGEEQEHQRTSANTTQLTSATTIQIPNHHNTRHYFTPSQQYDQYSNYSFSDIYNENLIIESFNSGINMMATNHQIMDNTSSIQTTGTTPPTNNITDSMIEEKIDLLDHHHDHYNIIESIKDQQTNSQQQQKQEHQTNSISPSSSPQELHHHHHHHHVNTTTATTFHSNLFDTFHRKMNHHKHHLNLKNQQHFQLEEVSSNEDNNDIDHRNQVERDEITSSTSSSSSSSREFSVISMRGDSDDTTIAEMDDISIDNDLQKSELKYCQTCKIYRPPRSSHCSICNNCVSKFDHHCVWIGNCIGERNYRYFLYFLYSTLISCILVLVMSIYTLHILVLKEPDTTTQDSTNSNSSNQQQTEIDKFINILVHDPKGGIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYS0</accession>
    <name>A0A8J4PYS0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Prohibitin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_006885</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the prohibitin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000337">
        <property type="protein sequence ID" value="KAF2071801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYS0"/>
    <dbReference type="OrthoDB" id="275637at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03401">
        <property type="entry name" value="SPFH_prohibitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000001">
        <property type="entry name" value="Prohibitin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000163">
        <property type="entry name" value="Prohibitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222">
        <property type="entry name" value="PROHIBITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222:SF1">
        <property type="entry name" value="PROHIBITIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00679">
        <property type="entry name" value="PROHIBITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="Band 7" evidence="7">
        <location>
            <begin position="41"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2071801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="292" mass="32703" checksum="157DFC0E9F02435C" modified="2022-05-25" version="1">MNMNFGKQFKNLPKFQAPKGGLGGGLGLAALVGFSILGFNSLVNVEGGHRAIVFNRVSGIKNRIYNEGTHIIIPWFERAEIFDVRAKPRSISSLTGSKDLQMVNITIRVLSKPSVNQLPTIYRTLGRDYDERVLPSIVNEVLKSIVAQFNASQLITQREQVSRLIFKRLVERARDFNIELDDVSITHLNFGKEYAAAIESKQVAQQEAERAKFLVEKALQDKRSIIVKAEGEAQSAKLISDSIKQNPSFLQLRKIEAAREIAQIVSRSPNKLFVNTEGLLLNLNDIEDKSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYX7</accession>
    <name>A0A8J4PYX7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phospholipase B-like</fullName>
            <ecNumber evidence="9">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_002165</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Putative phospholipase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the phospholipase B-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="9"/>
    <dbReference type="EMBL" id="AJWJ01000057">
        <property type="protein sequence ID" value="KAF2076551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYX7"/>
    <dbReference type="OrthoDB" id="443524at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007000">
        <property type="entry name" value="PLipase_B-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12370:SF21">
        <property type="entry name" value="PHOSPHOLIPASE B-LIKE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12370">
        <property type="entry name" value="PHOSPHOLIPASE B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04916">
        <property type="entry name" value="Phospholip_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7 9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035342173" description="Phospholipase B-like" evidence="9">
        <location>
            <begin position="22"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2076551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="556" mass="63187" checksum="CE148397F54D6BA5" modified="2022-05-25" version="1">MKLINLISTLLFASSIVCVLSIDPKTFENQFNKDPTSPSQWYSLLPGNKVVPGENVTGSIAWGYFKDELFLDGWGKLSIETVSTVSDEIAFTGAGYLEGYLSWNQIYNFSVNYFSTFLNTTNAKEFPIEVVEFVSNNYQYMDEQVKAASPTDPYWIQVANAMSQLNGLYNGYNDATVGTEYQLSFLEIYMLNLYGDMGDIISATMSDNTEFTPLSRKDVEQKMATDGHCTALIKLAPNNAELYVAHTTWAELAQMSRIYKRIKIPVASTPYGSETLFASYPALLVSIDDFYMIRPSQLMLTETLNSLLNNTLYSVVTPNSFLYWVRNLVANRLSNSGFEWVNYFVPYNSGTNNIQFMIVDYKLFTPNQPIQPDTLWIVEQYPGGYVAADVSFSLIENSYWASYNRPYSQEVYDIMGYPYYYEKYGDLFSWGFNPRANIFRRDQGNIDSLQGMQSIISYNEYKTDPLSDGYPGNAIAARYDLKAPPRHPYSWFYWGPHGGIDGKVMNKAMFDSFTVLAINGPTVTDECPPFSWSEFPDVSHVGLPEVFQFSWVTINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZD0</accession>
    <name>A0A8J4PZD0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lipoprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002428</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000067">
        <property type="protein sequence ID" value="KAF2076250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZD0"/>
    <dbReference type="OrthoDB" id="15547at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035303231" description="Lipoprotein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="14671" checksum="DA247501335DE739" modified="2022-05-25" version="1">MKNIIINFLLVLLFAQCIFCDTFFISRSGGVNEEYKSMEIDVNTGFYFDQSNHKGKVSEDDIRTIDNLIDSDAFKNIEPKYINQLENNQIYRYAFTSKKIGVIFDLNANYPKILDPVVLIFEKYSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZG1</accession>
    <name>A0A8J4PZG1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SKICH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000614</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000012">
        <property type="protein sequence ID" value="KAF2078063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZG1"/>
    <dbReference type="OrthoDB" id="20939at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2840">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="68"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="151"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="717" mass="83252" checksum="AF1CC3FB7334AD9E" modified="2022-05-25" version="1">MSQQPLDLDTEEKLLCLVEAFKHDRNVIFEILKKNNYSLDAAALELSELPSPPTSPPTRVLESQQQFIPPPNYYAQPPPGVQPQPVQPSTQPPKSNDKKTLMKDIASLLGNTELDQSTIYAVYQQSNNSIDETVKLIKDMTVDSNPTEQMRKEYEAQLQFERQLIEEQKRKETEALIRQQQQQQILLQERQMQEEKRLYEQMKVQQMIAMEKAKAEAEHRAKVEAEQKAKTEAEQKAKAEAERKARVQAEKMAADQDRLERERMEQFRLQQQKVQIEEMRKKQKEEQARLEEEKKARLLSLEVAKIQFEETRKLEQARQEKENLEKQIQIMQDMITQKEMNHLQVIQQQQQQIVDFEQKTKEIEILRQKLEKEQEGQMREKLNRENEELLKKQILELEAQKEAQDKQLLELDAIQKGKDKLETMLKDTQQQLLSHHVHVYSTLSPTNPEEIIVNWSLGSKFEPNTAYWIGIYPTHQPKHERYLAFKSISSASGEISFPGIIPGHYIARLFKDKYTFIQDSKESVKIGIDVNLAVHIIGDEINVTFNVSGGGDNYPIYRDWIGIYSQNQRNKKYITTLYANTNGSVTFKSPRAPGHYEIRYFAHPTKYNEQAKHSFELVDNDRIEISTPVASPGEAFRVEYTINTSPPSTSDWVGIFSVDETNNKNYILSKYTNGTGTGEINFTAPTQQGEYEARFFSYSKGKYIVHKVSNKITVTPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZJ2</accession>
    <name>A0A8J4PZJ2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003549</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000112">
        <property type="protein sequence ID" value="KAF2075159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZJ2"/>
    <dbReference type="OrthoDB" id="2123049at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2075159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="43306" checksum="7F8629BA8F658E27" modified="2022-05-25" version="1">MLDIKSIYRKEIEYDFNVLIVGEIFKSTNKHLLIGGVDGKIRVYLNKNEVETLETKAGAIQHLELFDTTRFGSIDIISGDNSGNVIIFSNHQILYKDTLNGSVTSIVTHQSSNNDFCIAVGNNNGIISTMKPHQPLLWKYQLPLTSAIIDTLSQSDVLRDSRDNLELYRQHQINSEGLGSGGTSEYKSVVQIKSVKGLDSFNNDFSFLIVSENTCFVHIIDQGKTLCSIPTPSPVNCMCVGYFDSRYSSDKQIALGCENGFVYILHDDFKLDLYCQIGYPITSLLKLNTSDIKKEDNNNSNSNDEDSDSDNSDSLFISGKKIIVDYLFCTGNFNSLKVLKDRNMITEYKTDDWIHTLSIGDVENNSEKMIVIGKLNNTIEFLKISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZK7</accession>
    <name>A0A8J4PZK7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_002290</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000062">
        <property type="protein sequence ID" value="KAF2076385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10263633at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1120">
        <property type="entry name" value="Carboxypeptidase-like, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055075">
        <property type="entry name" value="NOMO-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055074">
        <property type="entry name" value="NOMO1-3_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055073">
        <property type="entry name" value="NOMO1-like_9th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056190">
        <property type="entry name" value="NOMO_5th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056319">
        <property type="entry name" value="NOMO_7th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056187">
        <property type="entry name" value="NOMO_8th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051417">
        <property type="entry name" value="SDr/BOS_complex"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23303:SF14">
        <property type="entry name" value="BOS COMPLEX SUBUNIT NOMO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23303">
        <property type="entry name" value="CARBOXYPEPTIDASE REGULATORY REGION-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23141">
        <property type="entry name" value="Ig_NOMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22898">
        <property type="entry name" value="NOMO1-like_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22904">
        <property type="entry name" value="NOMO1-like_2nd"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22902">
        <property type="entry name" value="NOMO1-like_9th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23194">
        <property type="entry name" value="NOMO_5th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23660">
        <property type="entry name" value="NOMO_8th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="Carboxypeptidase regulatory domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49478">
        <property type="entry name" value="Cna protein B-type domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117074">
        <property type="entry name" value="Hypothetical protein PA1324"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035221951" evidence="9">
        <location>
            <begin position="23"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1219"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="domain" description="NOMO-like N-terminal beta-sandwich" evidence="10">
        <location>
            <begin position="86"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="NOMO second beta-sandwich" evidence="12">
        <location>
            <begin position="178"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="NOMO second beta-sandwich" evidence="12">
        <location>
            <begin position="382"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="NOMO fifth transthyretin-like" evidence="14">
        <location>
            <begin position="465"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="NOMO seventh transthyretin-like" evidence="13">
        <location>
            <begin position="690"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="domain" description="NOMO eighth prealbumin-like" evidence="15">
        <location>
            <begin position="757"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="domain" description="NOMO-like ninth beta-sandwich" evidence="11">
        <location>
            <begin position="847"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="domain" description="NOMO second beta-sandwich" evidence="12">
        <location>
            <begin position="1022"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF22902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2076385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1282" mass="146614" checksum="872E5C56ECBCAD7E" modified="2022-05-25" version="1">MKSAVFLLLIYLLLIVSSEVFSRDNSEINNTPQQQQSQDELNKDNNDIASSTSSHVLLNDCGGYIKLSKEFIVDQQNQQQNNKLIKNNINYKKIQVNLIEKSSGKIIETTVCSPNGYYQFPIYDYNYYQIQLIAPTGWSFDINLIIKDQCKDQDYNFELLGFKAEGYVYVDRNCISFNKQEKNIAGIDIILTDSKETKTIATTLTDSSGYYYFNNIVPGLEYRVIASHDTWSFIKPFTTFTSRVYDNYYIPQEDGGLVVGGYDMEGRLIDLKQQPVTTATFHLFYCSDYKNADGIAVERIEGCEPSQQAMHYDLDDRDPLCSVQSDSLGAFKFRNVPCGKYLVISEFYGPGPLFTLYDIEPRDHEYVMDSGGYAMEPGFKVSGFSLKGKVINHFGRGVRGVRISINGERKTTTNAQGEYILIGLLEGHYNIEATKKNYYFEEFQDYFVDEESDKIKPIKVLSYDLCGQVSFATPPPGVVVNPREIILGSKKYSEKKTLTDNLGYFCFSVTPGTYSLLLPISLEEKRRGMKFTTNSMTVTVEDEPVLGLTFSQLLFTLTGKIITLRKIKQHSEIPNSLIIELEQIQNSNSTEPLVVKEPVFATLVYPENGDIQFEFRNVLPGLYKVRVRNDVWCWGEAEREINLLTAEHDINDIEFHQMGYKFNIYSPNSNFISIAHRYNDQMLDTRILNKGHLNTYCLDRPGIHEFSVKSCFRFSQETYQFDTNSGVVGELLLNIEKYLISSNIIFDSSLINTTNVGEISFMVQIFDYQGLLLDLVETSFESNNVYHYSYWADLGDELEFVPIVNPVLVSKNPNEYKALLFYPKSIRVEVKQSDKCAPSVQEFSVKKGFYLQGSIIPPVEAVKIIATLNQETEEELGLDYRTSTAITFSDSNGQYRVGPLYDTNNYDIHAEKYGYLFKSENDGKNINFKTIELGNIIITVLEQSSQTPIPGVVLSMSGHDNDYKQEAQVPKNGSIGFHSLFPGTYLLSAAFKDYNFSPNKTTFHLYEGQEAKVTLYATKIAYSINGFIRSINLQKIPSNINIGLYSDDNLIKETLTNINGFYSFNGLAPGDYHMEVKDKQIYNSTPKRSNITVVDQDIEEIDFVIIFSKQTKTIDITGTIKVDRNLNHQNLYAQLYSQDNKLLQSIPINFFRSFDFKEVQLKTNPHRIVIERKLSDSKFLPLTNDIQPQFHLIDKDSCFAHYDFEILSNTMELPAENPILIPFSKLISTLIVLIIIAILIYPLKYLNFFEYVKEFFKQLKKKKSGSIKSRIFKQKKIKIIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZR2</accession>
    <name>A0A8J4PZR2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002689</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000077">
        <property type="protein sequence ID" value="KAF2075982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZR2"/>
    <dbReference type="OrthoDB" id="445357at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="120"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="191" mass="21239" checksum="732C8031D62E6076" modified="2022-05-25" version="1">MTNKKRNTSGQDNNKDEEDSSMNIDSNSSNSGGSSSSVLSSLPSEGTFTKYIKKPISDHIIPKYIPSHNTNPPNNEFVKNEEINILIRNLHKSNPLNKKNKRSIDTNDEEDDDVNGFEENQNQDQINNSQDNNNRSNNNNNNNSSSSDNGTRDSLPIITPPNPDKIVSPNNGKKKLRKTASKPTTTLSKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZV2</accession>
    <name>A0A8J4PZV2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_003330</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000103">
        <property type="protein sequence ID" value="KAF2075354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZV2"/>
    <dbReference type="OrthoDB" id="534815at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990423">
        <property type="term" value="C:RZZ complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046362">
        <property type="entry name" value="Zw10/DSL1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048343">
        <property type="entry name" value="ZW10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055148">
        <property type="entry name" value="ZW10_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048344">
        <property type="entry name" value="Zw10_middle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12205">
        <property type="entry name" value="CENTROMERE/KINETOCHORE PROTEIN ZW10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12205:SF0">
        <property type="entry name" value="CENTROMERE_KINETOCHORE PROTEIN ZW10 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20666">
        <property type="entry name" value="ZW10_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22766">
        <property type="entry name" value="ZW10_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20665">
        <property type="entry name" value="Zw10_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Centromere/kinetochore protein zw10 middle" evidence="2">
        <location>
            <begin position="365"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="Centromere/kinetochore protein zw10 C-terminal" evidence="3">
        <location>
            <begin position="518"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="domain" description="ZW10 C-terminal helical" evidence="4">
        <location>
            <begin position="666"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="57"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2075354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="814" mass="94389" checksum="1EA187162FA9D8BF" modified="2022-05-25" version="1">MMKDKGIENKKIVSTLLSENDPIKHLDSILNLSTPIISPTKQRRTLNLQKKKKRDNSNNATTTSGNNIDDDDDDLFHQTDKLESGVSSIISVLIDLKKSLFNTINTELEKSNTKKATIIHLQEEITTLETKKQELIHNIQDKNNGILSNIQESVNYDQELRERLSSIESILHILDYLGQYKNEITGFDDCVAKNDYIFANHHFQNMTNLMNHIPSQITSTHPHPRILPSLKDNLKKRKNLLKTTILELFTNAIVISPNEIQLSLGPIDQHVIHESSSPPSHHTFSSFVPKLVDSLNSLGLINFIISSISVNIFQTILQPLYFNSPPLSSSSATSPNDQPFYSITPKWTNQEEGDPFPKVYFEFNQDFKYISTEELYLVITNLINFICNNIFGSNKEYIKKFGDEFWNNISSSILEYSLKYRIPDDIKGLEWFSSLRDITIQFENHLSSIGLIKDQDKKLTSFVENIELHYSEKKRNILLFKAREIILNDRYKSIYSDESIFGEFQPNDGNISGYYFNNRIIFQSCHQILDLCKSAFKESFLSHSICQKSLYHGSRDILDIFYCIYLKHHKDKLETVPSLACLFYNSCMFLSHSCMFLALEFSSNQLVKKETFLDFSPRFKQLGNQFFSKYIKNQLESILSFIVEHCNQLDGTKDLLIFNKIESNFKRMILELSKLSETWKESFPREDYLELITKFVESIISIVIKMILNLQNIEVIETTRLVQLISIFSEFEKFFEFPGEGDVGKSRMKLVKNWKKLYQISTVLELSLSEIVNHYKQGLLKKILNHELRLLILSIFDDFELKTNFLAQLNKSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0A4</accession>
    <name>A0A8J4Q0A4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycoside hydrolase family 5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002360</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000064">
        <property type="protein sequence ID" value="KAF2076354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0A4"/>
    <dbReference type="OrthoDB" id="16991at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000297">
        <property type="entry name" value="Galactose-binding domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018087">
        <property type="entry name" value="Glyco_hydro_5_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34142">
        <property type="entry name" value="ENDO-BETA-1,4-GLUCANASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34142:SF4">
        <property type="entry name" value="GALACTOSE-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00659">
        <property type="entry name" value="GLYCOSYL_HYDROL_F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035191723" description="Glycoside hydrolase family 5 domain-containing protein" evidence="5">
        <location>
            <begin position="24"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="6">
        <location>
            <begin position="72"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2076354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="543" mass="61627" checksum="AF178855A97BA98E" modified="2022-05-25" version="1">MKKSSLLAIAILATLCLSSAVLAGEMSTAGLYVDGKYIKNEDNEILMLRGANRPGTEYSCVQYAKIFDGPYDAAHVHEIRNWKINVLRVPLNEDCWLGTNGPPESFYFGEQYRESIKKYVDMITSNNVAVIVDLHWASHNGTLATEQVAMPNNKNSITFWGQVADTFKSNSRVIFDLYNEPFPYGNTWDSEDAWQCWKDGTKCDVDNVTGMQQMVDAIRETGAKNIILLSGIQYATSFTRFIEYIPFDPLKQLGAALHSYDFNYCRSKGCWDVYLRPIYESYPLVATETGQKDCLYDFLLDFLHYCDKNSIHYLAWSWLTGDCNIPSLIQDYMGTPSNFGKGYKHHLDVLGNGEEPWWSDSFDMYNDKLTHWVDNWSTGDNIVNATAQTYAGKYSIQFTPSAEKSLHLMCWDCIKNQVHKSLEFWIHGGASGNQNLKLDLLKLQADKTNNIAQSYDLSNIAGGPIPPNKWVKVVVDLTTLPRQDSYDGFWLKADTDQPDIYVDSITVRAINEPPPDDFESSALRLPSPNTLIVFLFSILYLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0B2</accession>
    <name>A0A8J4Q0B2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000073</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000002">
        <property type="protein sequence ID" value="KAF2078573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0B2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2078573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="675" mass="80107" checksum="3191C9952445A7C0" modified="2022-05-25" version="1">MSIKPTSTSTSTIYQSSSFLPIVKNKYLFGLILNLCKYHDRVGYNYYDTPLDILFKRNRFDIFDHRWDLAFNSNNRIQLDIEHLLDISYGDIITFFKHNTSKERLTKVWPIFFPILQLVDLGQENDVVLCGLIIVECKFELQQFIEYQYSNEYISKKPITIESLEYFAKVIGSLGCIQIFGHSCKVFEKCFSNFPYNDFKYLCMVESSNAGKLNLFNRIISKYKPCEFKLDYNQYDMTKFPPSVSKAIMESGEPNIKINFDQFPFDNMSMEYMDYYGTDMKNREPKWSRNISFDHAYKIGDIKLLDYMYQTFDVQSKSFVVDHIPNLEVLNYYISKHNDLFTNRFSGLENACIHSLDLVKRLHSRGFNFSYLSFDACYEVLEYLNQKKIFDLIPSNFRAITKMYHYDLRIIKLFTQTYKDFYVKSSFFNEYVHRDCYDIVEYLLFEDSKQNRRQYSYGATLLSEIARNEKMFNLLLRHNQPVLQEISSKALQQFIPIMYSTMQCIKRTTKCKCLLSEFTKRGSDIWCFDPKEMFNPKVYECLLDGFSLEAADTDPSLYQHLTDIIKHAIKHSNFKLLRYSLSLLNYQGWRFFIQHFDLLEMNLYSIQEKTYSLIVNALSYELLVDHLLVLSKKKHYHPKPRLLAIISQILYIQYTNKEMLIPEPIVDLLANMTIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0I2</accession>
    <name>A0A8J4Q0I2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003099</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000093">
        <property type="protein sequence ID" value="KAF2075594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0I2"/>
    <dbReference type="OrthoDB" id="27226at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07516">
        <property type="entry name" value="HAD_Pase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000150">
        <property type="entry name" value="Cof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00099">
        <property type="entry name" value="Cof-subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10000:SF8">
        <property type="entry name" value="HAD SUPERFAMILY HYDROLASE-LIKE, TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10000">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01140">
        <property type="entry name" value="C2.B:_Phosphomannomutase_and_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01228">
        <property type="entry name" value="COF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01229">
        <property type="entry name" value="COF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2075594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="293" mass="32899" checksum="6F228A3923683F85" modified="2022-05-25" version="1">MYRVIALDLDGTILNSDKKVSEISKKVLQHLTSKTFKNANGQDVMVLLASGRAPYLVQPVEEEIEIDCNLIGYNGGLCLSTKSEGRSTLFSRSIEYPHMQQIFKYVDENNHFLNVYGESHVYGTSKAELRAKPDIYAKMTGATYKFFDHPYSELSESVAPTKSLIIFEDEQECDSMLEQLKPQFPEVSLVKSNCMTKEHKQFYVEFLQKGVNKGSAIRDFCKAKNISIDQVIAFGDAENDIEMLTEAGHGICLANGADQTKQAANYVSEFTNDQDGVARELIKVFNLPAELFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0M9</accession>
    <name>A0A8J4Q0M9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002151</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000057">
        <property type="protein sequence ID" value="KAF2076537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0M9"/>
    <dbReference type="OrthoDB" id="20265at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339:SF1">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0807">Transducer</keyword>
    <keyword evidence="2" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="480"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="170"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="649"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="690"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="171"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="182"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="198"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="242"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="265"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="701"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2076537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="813" mass="91306" checksum="637EAE6DEDBD35AF" modified="2022-05-25" version="1">MKFSNNFVLEHRRVKSFDNNLISSQQHSLATSITVNSSSLSPSPPSSSTLCSTKIVTNGNSKEQYQNKTNIPTTTVNNNNNLINNNNNNKHSIKNEKQLDCSIPFTIMKDDDIKLTNILDQNDFLNQPQQHQQHQHINNNNFILKCPINNNNNLNQIDAVNIKNIFHDQKASQSPEPSSLNTFTPTSSFCTSSSSTPINHNIENTNNYSKGFILTPPPPSSSPLHTQITTSTSDDDHHHQKTTTTTTTTSTTSTPPSPLKSQQKNLNHQNLKSESPKQSPLIHFSSCINLKSFVSDCEPISNRGQSTNNNLTDSDNVFLKCNSLKRNLQISNTYNNSDSEITQSQPLEHSLDNFIPISKKSVHTNTSQIYNKTNINNINIQESMIDQSNNIKLSNNNNNKNNHLNNSNNNIILNSNNYSRNTNNITMYSITNDNNNSCNNQHIQGENIVHINNSKNSEINNNNNNSSNNNSNTIEKKKAMILVAEDDPLNRMIALNYLGRLGHTVTLAENGLQAYNLVKEKQFDLILMDVQMPIMDGLQSTSKIREYQESLGKDNNPRTPIIGLTTISRDECLSAGMDEHLCKPAKFQQLDRMISSFLNKSVYHSCPSSPSNHLFSTSTPDLLQYTNKPNRDKLISTIQEQVQILPDKASEAVLSPSTTTTTTTTNHSYQKISTPRILDKFSLIKIDDNSNDCKNSDHNNDNSNNSNNSNNNDIIINNSNNNNKTDKEDDNNDNNQSISHMRVNSDKLATPKVTNAFFSKLSLTESFNSLDVNINSSNNNYTNDDENDKQEIFVIKVSRSNSEPSFYSKKEYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0R4</accession>
    <name>A0A8J4Q0R4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_002745</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000079">
        <property type="protein sequence ID" value="KAF2075942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0R4"/>
    <dbReference type="OrthoDB" id="10250130at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF18">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="F-box" evidence="4">
        <location>
            <begin position="105"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2075942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="525" mass="60004" checksum="7F47B9DD54243D78" modified="2022-05-25" version="1">MGSVVSCISENKKNNRSTDSPSTSRGGKKEWKKGSSNSTLTVSISVDEDGNKEHQQDLDIETYCDYEYIPNHNDKRMISKSLNDIQLNRFNLVKSSEFYLNQRQFKLFTSLPQEVQVFILQCLPARDLIQISQTNHFYNQLSKERTMWRERCFSKDWIIQNQFDTKFNFKSYYFEKNALNSKNFAKWVSPKFFGQLPSKRFKHTATCFGKKVLFIGGQETDTKRFNEIIYYDTETQTFSKPSVKGDRVPNFSRHSASLVHDKIYIFGGFDGHGTNFDLATYNTSNRYWTNIPKEAIKGSLPHSRTNHASAVVGKNVYIFGGNNNDENGRYQVLDDLHVLNTNELEWSQPNVTGDKPCARSGHCMTSIGTKLYLFGGGVWNEDEGWVEKFNDIHIFDTETHVWTKPPVKGDVQTSTFAISFNVGRFLFIFGGGSKPGHCVTNEIYVLDTETNYWIVPSIEDPSPPARDMGTACVCNGDVYFLGGYSGGPIDYFNKLKFNYKVLANLAKDQRQDLIQINQKQGECVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1V1</accession>
    <name>A0A8J4Q1V1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">S60 ribosomal protein L11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_002309</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000062">
        <property type="protein sequence ID" value="KAF2076404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="OrthoDB" id="1734943at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="60S ribosomal protein L11, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_uL5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_uL5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020929">
        <property type="entry name" value="Ribosomal_uL5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057266">
        <property type="entry name" value="Ribosomal_uL5_euk/arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_uL5_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003258">
        <property type="entry name" value="PRK04219.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00358">
        <property type="entry name" value="RIBOSOMAL_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL5 N-terminal" evidence="12">
        <location>
            <begin position="30"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL5 C-terminal" evidence="13">
        <location>
            <begin position="87"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2076404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="197" mass="22107" checksum="D40155F07CD674D9" modified="2022-05-25" version="1">MSKAATKNATKVASKAPETAAPVEVTKKDNPMRGLRIEKLVLNICVGESGDRLVRAAKVLEQLTGQTPIYSKARYTVRSFNIRRNEQIACHVTVRGEKAVEILEKGLKVREFELRNRNFSNNGSFGFGIQEHIDLGIKYDPSIGIYGMDFFVVLSRPGFRVTNKKRIQSKIGFQHRITKSDSVAWFKSTYDGIVIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2K7</accession>
    <name>A0A8J4Q2K7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000025">
        <property type="protein sequence ID" value="KAF2077575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2K7"/>
    <dbReference type="OrthoDB" id="23630at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052145">
        <property type="entry name" value="Mediator/Homeobox_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24330">
        <property type="entry name" value="HOMEOBOX PROTEIN BARH-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24330:SF19">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="364"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="468"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="725"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="332"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="364"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="404"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="432"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="472"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="725"/>
            <end position="752"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="906" mass="102333" checksum="D3F9610323F2BCD1" modified="2022-05-25" version="1">MDDFKPFSTRKRVQTRLIAPVDNNNNNNNNNNNNNNNNNNNNNNNKSDSNNNNIDNNMQNINNIINVDQDNILALEQSILPSSETIFVNNEFIPNSSLFGQNHEIDLTHVDQILIQLQSEIQNQQVQEQQQQQQANNSVGTPKIVKMLEIMEPEIQRSASNTDEVVNLLSKLDVSIVDTNSQGEDVDGDDDKESNFIDDGEIVNTKNIIILDEEDEDDEDDDGQGNGNPEAEVAVAKGDADGDNDKDNEDPDPIEFDQEDLVVGEKHLVIHEKVKERNNRQRLLREIKLNEMFDQITNGEPAPYITPTKFTKRRKKKDEEDQEDEQDILGYNNNNNSNSSRPYSSCSLPDINSINHLLVSQPSYNNQPASYSQRHISVPLPPPIQQQQPSAPPITPTKFFLNPSIFNSTPPRSDTNNSFKRKDHPEPSPIMSRTSSTYTSTESKTIPFILDSPVKSNPIKPLTANSTAFVPQQQQQQQQQQQQQQQQQQQQQPNPVFKVPLPPSQQQQQHPSIVRWKKLFSFKTSLHRKIREAITGASILSSKLLINNAAHLNVDYVILASLRLFLLVPSILNSTPKNEEGRLFTDELNNNTLMSKLKDGFKVKDIITSYKEITTDQWLSSKLHTPKGLLEVDKFNQILELVISTISHDDLLLSIISIPTTTQYMTINGFTVQASNPTQGLEIQKLLESMKRVVTTTRLNNLYNRYQSKEIQAIINSKNDNNIVAPPTTTVNTTTTSTTPNISNHNNNSPSNVEEPKNNGQSIKPPNFYPKSPEKVTSSISNNNSNQVNGFKPPLPANTTPTATTTATATIPQQAPTITNNIDQIKSQIELISDPKEKLDKLYQVIEDQNKFINQLQADNQVLVANNGTLFNNLQAYMNWQHSIKYKYDEFNSFMTHAMSTFQLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3R4</accession>
    <name>A0A8J4Q3R4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">IPT/TIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_001115</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000025">
        <property type="protein sequence ID" value="KAF2077574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="20492at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052014">
        <property type="entry name" value="Dictyostelium_Tiger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31341">
        <property type="entry name" value="IPT/TIG DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1847"/>
            <end position="1871"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="4">
        <location>
            <begin position="262"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="4">
        <location>
            <begin position="571"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="4">
        <location>
            <begin position="653"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="4">
        <location>
            <begin position="900"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="4">
        <location>
            <begin position="989"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="4">
        <location>
            <begin position="1065"/>
            <end position="1140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2077574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1890" mass="204515" checksum="DA5B59599ABF10B5" modified="2022-05-25" version="1">MAATNNYYFTLFVLLFSSVFLFCNVNAVYTLKQFPLNQHWYERIEERVSGINATLFCEQKTYNGLKGYLATFSFEEEWAFTQANGVTTIATAWISGSDAVKTGFWTYSSGPEKGQPMYNLFFDKSYSFSFFGGIEPNLIPNEHWVHTSGNAAAPYWNNIATTSNISAYICEYGGLDDPVMPTITTTGGRVIITKLTGYIVSSLTITLTNRVTPTNTFQCGSIQVINSTSVSCLMPVGTGRYSVLIIDSAKKNHTLLWQYELPFIQSVNPFFKKDESITLAGNNFGTVASAISVLVGTSQTVVCGSVTILSPHVSISCKLPADLTEKLLPISVTVNSLKHITYKAAIFSPKNKLYYSGFLTNANWATSTDGYVKNLRVDGINAHLAVLDSPDLWTFLNNTLPIPFAGNLWNVWLGVTYNPTSKKFFYLAGPKAGTQAALQYTSIAGDQTTWTADTRFVVNLYTGVLQGVLPTAASGTFAEYGGEDPAFTTNKTHGIDTSGGSIGIQIDNYGTVFSVLSVLFRSASVSFVRDFINSELDVNIPIGYDGPYQISVTVDGKPTAPNTQFIDYNPPVISSIPSISTRGGDVTITGTSFFNDITKNTLTFGSTPCLAPTLVTAHKVLKCTMPVGTGVRSTTVKVGGKTSAPFTFSYIKPSVTTVSSVSPAGGTVTIDGDNFGADATKITVSVGSFACTGVVLTTAHTQFTCSVPQGQASHAVTVTVDTLVSNNNILLIYNSPTISSAVQISDGIEITGTNFGTDLYQLKLFIGASEITSYCKSTMIKVTCTALPATISSGAITTTGAYVNPSYNFLLTPFVVSVDPTSVATSGGAVSIKGKFFETTAMGTPTTLSVIFNGVTYSNNGYQSSSLLVFSVPAGTGVGKSISVKTDTRSSNSKTIAYIAPKVTSHSQTDETISLVGTNFGNDKSKISISIGSVPIDSATLVLTSHTQLSFKAPLESLNGGLLITVDTQSFTYSRFTLKPVITLIPAPKVIGEAITIQGKYLSNVDQDSLSTNIKFTFNSVVSECTFISATSYECSAPPGTGKAVAFVVNELVRSIDTQYLYQGPTIETVTHTIYNVPQEITISGTNFASTGLLVTIGDQDCQSPVAIDDTIITCQFESNATPGQGNLDVSVTVDGQSATEAIFAYASFSVSKVSQVQDYLQISGFNFGDVSKLEIKIGEVALTTCVGNNTFITCNPLPIDTKSGYFDITNGPSPITPIKVLLKPFIYTISPTTIKTVGENITMTGRFFEATSIAENNVIHVFKSTYESIEFDLIDNTTLIAKDIVGSGSSNSLEILIKNRKSNVKQFSYIPPTISTVQHVDTVLNVTGSNIGNQSSITIYYNGQEFNATNIDSQWFTITILENSTNGELYIRVADQESNKIRVLLKPNIKSIAPSPYTTGGSISLSGRFWNIIDSSNSPIEYKFYYSLLQSTDKSFKLLACEAITKAYTVVCKQPSGYGNFSVKVIAEGVESNQFVLNYQIPIVFSSSSLFYNTPGNITIHAQSIASPSSVFINNKECTNAVLTDTEHIRCFYDASVAPNQNGDPLTVTVTSYGLSGKNDVYLYLKERRCPGIPECSANGDCDTHTGVCRCYNSTITTSDCSIVDPNVLPPTTDENGETTLPTTGNFNYTIAITHLREKNLKEETVKTIKVSTIHWVDRKKLSEHTNYYKGGFENDPAILELQVTYFPTDSSIDFAGDILSMPANSVKYEITVSNWTFTSSMNYLQVIYNSRTERTTTYECVDHPTNITKLNVESLSWFQIVSGGNTLNSKFADRIIVDSRIVGSNVKLLEQTDELYNDLRQDSNTFNILTAMSTPYFKKNVVVDPNFSALLKTSTKTECDSSEKWKIPVIVVLSTAGGVSIATAAAVFYKKKLAFKKMEKSISMKSVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3V1</accession>
    <name>A0A8J4Q3V1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001036</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000023">
        <property type="protein sequence ID" value="KAF2077649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3V1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="111" mass="11577" checksum="FDCD9A406C33661F" modified="2022-05-25" version="1">MTIFNSISSIGLNKISTSLSNTQNASNGLNLSTENQSSKRLMCCCVGPCIISSVGILSPPSSNKAVSNSITQASKEGSNELTIVCNNPCDFCVDAKKGSTFWNVICKHGAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4E9</accession>
    <name>A0A8J4Q4E9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetylglucosaminylphosphatidylinositol deacetylase</fullName>
            <ecNumber evidence="2">3.5.1.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000662</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PIGL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.89" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000013">
        <property type="protein sequence ID" value="KAF2078024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4E9"/>
    <dbReference type="OrthoDB" id="440160at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000225">
        <property type="term" value="F:N-acetylglucosaminylphosphatidylinositol deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10320">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003737">
        <property type="entry name" value="GlcNAc_PI_deacetylase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024078">
        <property type="entry name" value="LmbE-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993:SF11">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02585">
        <property type="entry name" value="PIG-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102588">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="279" mass="32226" checksum="D3DC843B5D02ED05" modified="2022-05-25" version="1">MDYFKKKRDASVKRTVAFVIAHPDDECMFFTPTILHYKRMEYNIIVLCISSGNQAGLGRFRSIELVKSCNELGIPGKNVIVIDWPTLQDGMDQHWTAADVASLLEEMTTRYAVDEIVTFDEWGVSGHPNHCDTHRGVIQFLQNYQQARSKNLPHTHRKQVEDQKQNKTAGQMKEIRGYKLETVGIIRKYIGIWDIWVSKLFGAIDGDSYHAKHNNSTDSSSSSHSNSNPTIMYTSTQLFPPCSYDPMKQHQTQLVWFRYLFIFLSRYSYINTLTEIKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4F4</accession>
    <name>A0A8J4Q4F4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zinc/iron permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_004938</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000185">
        <property type="protein sequence ID" value="KAF2073736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4F4"/>
    <dbReference type="OrthoDB" id="448280at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF44">
        <property type="entry name" value="PROTEIN ZNTC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="234"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="333"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="134"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="155"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="175"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2073736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="352" mass="38173" checksum="54571EBAC3765DF0" modified="2022-05-25" version="1">MMRKLLHGTHEHEGSGDDLEELRNPKIGLIVGIFFMTLLASYVPWIIGRAKVKNFVTIISISTCFAAGIIVAGGFAHILPGAEADFAEYWEHKDPENRYSGFPFPITIAIFVMFILLAIDKIVVEKGITGEKGHNHMNLSEHSQNYHNHTTSHTGEIDLKDVKEGAEETTPGGPDEEKGFKHHEDDGHGHHSHKHGNGKSHEANTSQAYLFLVALSVHSILDGLGLGTETHSEGFYGLLVAVLAHKLLDGFALGVPIYFANFSFAFSSACLAFCAAMTPLGIGIGMAISTVYDSEAAYLAKGIVLSVTLGSFIYISLIELLPSGLCTPGWIKTKLALAFLGWSIMSILALWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q997</accession>
    <name>A0A8J4Q997_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_001556</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000037">
        <property type="protein sequence ID" value="KAF2077171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q997"/>
    <dbReference type="OrthoDB" id="6105938at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047134">
        <property type="entry name" value="RNF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23041:SF78">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23041">
        <property type="entry name" value="RING FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="362"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="27"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="105"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="200"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="248"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="67"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="115"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="273"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
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            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="340"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2077171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="414" mass="45979" checksum="C497AA01A43B11C4" modified="2022-05-25" version="1">MNNNNSGGHDNSYDLQIRDQLLRMLAENAPPDRYFPASHINPYNQEDDSDDEDYTDSEEGDEEEFNFGTSPAASSVSAEENEGYEPGHLVPFTFPDSYHHATLLEPNNNNEIPITTTTTTTTTTTTTTANPLVPLRLGGSAEIIELDSDDVVETTKSSEEVIDLTEITTSPQVQNTTLLPASPPYLPLRFPHLSTSINNNNNNNNNNNNIINNNNNNNSNSDTTNRMQQHAFPYTSYYGPPVIVQIINGDRQNKKRRNEGEENEDDDEPVIVGTNNRSFSSFINANNGIPPPNKRLSSGGNHGNNSVETIHISENGPALSDRSEVWRLQQEFEKEKAQKEANNNNNNNSSNNNNGKKKSLECPICFDEMDSMMSTTCGHVFCNDCISKALKKKKVCPVCNSKLHGPKSLHPLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPN7</accession>
    <name>A0A8J4UPN7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009203</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000654">
        <property type="protein sequence ID" value="KAF2069481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UPN7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="68" mass="7002" checksum="2105CD65EE87DB8D" modified="2022-05-25" version="1">MMSSNNKQTDGTRQGPGGSGGEFRRIPATGPSATKGTYNATIDPGLQLPSSSAGSPEDFYSAPQDQPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UU41</accession>
    <name>A0A8J4UU41_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA-directed RNA polymerase</fullName>
            <ecNumber evidence="2">2.7.7.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_003683</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RdRP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.48" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000118">
        <property type="protein sequence ID" value="KAF2075031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031380">
        <property type="term" value="C:nuclear RNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
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        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="RDRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058752">
        <property type="entry name" value="RDRP_C_head"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057596">
        <property type="entry name" value="RDRP_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23079">
        <property type="entry name" value="RNA-DEPENDENT RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23079:SF55">
        <property type="entry name" value="RNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05183">
        <property type="entry name" value="RdRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26253">
        <property type="entry name" value="RdRP_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0696">RNA-directed RNA polymerase</keyword>
    <keyword evidence="9" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="277"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="623"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="175"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="215"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2075031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="2289" mass="265189" checksum="8E78984E96C70CCC" modified="2022-05-25" version="1">MISSAATELSKYKFIHCLCGECIVNFDEIYFVQDKNDNLSLEIDNLAGYNKTWGFIVYDESIGGANSSMQIDDDDDQVDGEEIFFNKAPNEFLMYKEFIPKDSSDLSRLVDNRRLFLSCLYCHETIGYHKIRLKSERGYSLCLIATAVTIQFDDPLDSWIEALHQYSGDIPVKQERQLELQYQLKQQQLQQQQQQQQQQQQQQNKLSSIDTSSSYTISSASSTSSTTTHSNISPLGPLTSIPSVSKIYRPTTYPKDEFPTPPSLCGGVEPYDYQIESFHVAIKQNTILILPTGMGKTLISILTLLEMYKLNGSIIDTQENKDIQIFEKRISLFLVDRVPLARQQANAIEKQTNLKVLVCHGEIKTRELLTKIYKKEYDVLVGTVDSILNVIDDGYLNISDFYFITFDEIHHANPGHGYVRIMDQLNRLDFNLHPRILGLTASIVENSLDLYRCMDQIRVLQTTMKCLIFKPLSLQQEESADNDDKGYSKSKFEPTIHSIKEAPYQDVFEKEIHDCLLKSIKEFQKEIDYVDLDLDSVAYSSRFLYGLKKLKFKVSSINDPAMDKKIQNILDIYDLISILMTQGPFATLLRFKEIEIQFPSVGPYIENIEEVYPIDSVPSNCLRFDKLLYVLSQSVTLPNFDKDFRCVIFVETRACARALVKLLNDSKYSVLCPQMFVGHAQYDGMDVNTQMTMIQRFIDGHTRLLISTSVLEEGIDIKKINTVICYDGVTSLRSFIQRRGRVRAKDENISYSILTNDSQLFSVQEIIRQENIMDLAISELIKSKEIQDKKDMLKYQHTKLTFSDKSGSSGGANGISTETITMGFHCVAKVLEFKNFVKQTYAATLLGISENYESDMDEFKSHYTILHFRKPKASIPAFMFSVYELLCKTFAFWMYYPSNPDLKLPNRFRFGMRCNFSHGNMKSPNCFVATKQEQYDTNIAIRKTQLEFTFGKFTTYQKYENIEKYIVYDSATQYNQVDSIYIFIIRPCVVNEVKGDKHHDRVNSPLGQSFAYRFTFQKHLREETMNNLLKVGFQLYFGAVDVQRSNTYSDFSIGSVYSVGNAEVSYLLHALATNRRFGSFEINDLFVEKIKQIVWNQGESVAKALIQYAMFCNEKFITNEAIFDNIDKAKSIYRDINTSLVNPHHLVTKSISVTPSRIIVHPPDEKLGCRILRHFGHQHFLLVRFCDEKEEKFGFLSKPLQKYVLDILTKGIKVNGKHYHYLGSSKSQQRESTVWMVEGLDVPTIRDWAGKNIKKDPRVFLFRFALQFSTTIPFEYIPPYHFHRIKDILNAKGDKNFTDGCGFIGKKILARAVKQLNYDPTVCAFQVRIGGCKGVLVLNEHLDDPEGIYLTESMIKYETEEHVRMHRTLEIISTNTATKCQLNKQFINLLSHLGTPDKYFFKLLLNQMVSSSQSLYNWEMAQGLVCKFLPESVSQDSHSLLEDIFIKRILNMHYKKNLETCLDKLSIQVERSRNLLGACDPYNLLKPNQVFIQIIEVNEQGERVPKPLEGRVVIGRPPCLLPSDIRLVDAVDIPQLRSHINIVLFSTQGEIPTCFQTSGGDLDGDRYFVFYDSKIIPTISPEYIPMEDEASQSPKLDPHIYYSNSLAEKFVENLSKSYLSQIANHHLAMCDLFSPEDLLCINLAKQHFIEVDSSKTGKHGKVDSQSRKIIKIHGYPHFMDRGDPNRTYYPSTKVMGKLYDQLKSLWIADFLHETKVKKENLVEGYEKYLKQAELSYSDYQKRINGLKNRFGAKAEEELLIGFYSDSRSRHAFNDRIKDEIKNLYLIEKKKLQEEFIKEFYTSSIDNTLLIHRKEIERKISAWYFVAYSDRSKNSVLSFYTFVSSIYKSNQLGYDQHKIRTNNLLEYFLNKSKSILESYTQCLDLAKQIQQLLRLSKIQPFTQCRVLLTGSFSTMLFDPVHSSIDLVIRVNGDIDKQSLTSKLDKIVKGSSLPIKRFIYDTAAPSFSFTTYSRKNVKFSFENQYQQNQTQNSMKILKLIHSNPYLLPFIHIILDWGKESNLIEDDDPKYSNNAGVGELNIQRLQRWKIPWIVIDFFHQNKYLKTFGQIDEERIGQSVEWWQNVIKRSQNYDFSIEKGGYLGEKLLKFFKDMTKTFGSLLYKNDKPYSVNYPFSNASPIFSFGAQGLAETHQYYYNQFFGGFHCISNTIDITEFLANSCGHECRKTLKYKKREQYDTFFRDKLRHSLEKVHCKLEYTQEKKNVVITISGNNFFVVLGTHMARKQFDQIEHKQMKIKYDSDDEEDYDQNLEYYLDEPLYDLEDLEYDNDFYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UUC0</accession>
    <name>A0A8J4UUC0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_003415</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000106">
        <property type="protein sequence ID" value="KAF2075286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UUC0"/>
    <dbReference type="OrthoDB" id="1367865at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.960.30:FF:000001">
        <property type="entry name" value="F-box-like/WD repeat-containing protein TBL1XR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000218">
        <property type="entry name" value="WD40 repeat-containing protein HOS15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045183">
        <property type="entry name" value="Ebi-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22846:SF2">
        <property type="entry name" value="F-BOX-LIKE_WD REPEAT-CONTAINING PROTEIN EBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22846">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="187"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="243"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="284"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="367"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="408"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="459"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="98"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="98"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="124"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2075286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="59338" checksum="10F361390CCF67F6" modified="2022-05-25" version="1">MNITSDEVNFLVYKYLLESGFQHSAFSFYNESFISKSEINAANVPNGSLVSVLQKGLQYMEVETHLQEDGTEVHCNEPFSLITPHVCQIKKKSNTASTAASAAQSSNKENTNTSSSSTGTAAANKDKDKDKKDKYKDKDKDKDKDTRMSDVIPMDTSSNTTTTTTTTTSTASNTNSSEIPESQVQTLVGHRNEVYICAWNPTCSLLASGGGDSTARIWKIPSGPCESAQHSNPTILYHDNAVINEKNIDVTTLEWNSNGQLLATGSYDGLARVWTQNGDLLHMFNQHTAPIFSLKWNRKGNYLLSASVDKTSIVWDVKTGTVKQQFEFHTAPTLDIDWRNNNQFATCSTDKMIYVCEIGKNRPIQHFRGHSDEINAIKWDPTGSLLASCSDDSTAKIWSMKTGDCLHNFQHSKEIYTVKWSPTGPETENPNKKLILASGSFDSTIKLWDVDVGKCIYTLEKHQDPVYTVSFSPNGEYLASGSYDKYLHIWSVKDGTLIKSYKGSGGIFEVCWNNTGDKISACFSNSTVCVIDFRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UV97</accession>
    <name>A0A8J4UV97_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">VPS10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_002338</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000063">
        <property type="protein sequence ID" value="KAF2076381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UV97"/>
    <dbReference type="OrthoDB" id="443634at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006892">
        <property type="term" value="P:post-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031777">
        <property type="entry name" value="Sortilin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031778">
        <property type="entry name" value="Sortilin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006581">
        <property type="entry name" value="VPS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050310">
        <property type="entry name" value="VPS10-sortilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106">
        <property type="entry name" value="SORTILIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106:SF27">
        <property type="entry name" value="SORTILIN-RELATED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15902">
        <property type="entry name" value="Sortilin-Vps10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15901">
        <property type="entry name" value="Sortilin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00602">
        <property type="entry name" value="VPS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110296">
        <property type="entry name" value="Oligoxyloglucan reducing end-specific cellobiohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035323506" description="VPS10 domain-containing protein" evidence="6">
        <location>
            <begin position="20"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="664"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="domain" description="VPS10" evidence="7">
        <location>
            <begin position="40"/>
            <end position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2076381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="742" mass="80974" checksum="E4E517FA67F82941" modified="2022-05-25" version="1">MKGLSILLFFCLIAALAIATPSVQKYQFKWKTSPVYLNSDVALIRDDNDNYWRTGDGGITWNIIKTGNDPVKAYHMILDNFDKNLVLLLQSTSINGGQTKLFVSQDQGKTFVIASYSGPLSRLLPSPKQSRYLLAHSGSKMVLSTDFGISWKDISGGKALYKAGLVLPPYWDPVSGGIFAVLDKFENSLPWLTFTNDTGKSFTKLVSNVIEINSTPQFTYLGVSDAKNVGQSFLYVRSNTQPVNNNQMGFLECSFPFGNDVIANEFRIIDDTDGAIWMGVVLYSLNKNANRYGNIYVSNDRGNVYTLSKENVAFLGDSFDFSPLFGVPGSFLANVVVNTGVDSSQPLLTQSWITYDNGGRWNRLTAPNDTETDTCTNCYLNVHGLSAFTDFTRGYGPFYTIENGPGLIVATGTIKPALSTQPKTDRIKTFLSRDNGLNWSTIYKTGTIYEFGAYGSLLVMADCVTGDTNELLYSFDQGKTVKTVQMPGAYDIINIVTDPQNANTKFIILAQDSNDVATVLSLDISGADLPPACTDNDYLDFQSTCLLGEQITYSVVAPGKSCVITTKPEASAVTICNCTVDDFECDIGYTETGNDTSTLVCALTGNTNSSSTKTDCDNGAKEYTVTRGFRKVAGSKCQGGVENQYKPSTLMCPGTASQKKSKGWIAAVVILILVALLGGAGFFLYKNPDKLELIKKKIGISKETKYSFIGIKPNSLADDEFGIEDDDAQILNDNDLQDDDNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVK4</accession>
    <name>A0A8J4UVK4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002036</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000053">
        <property type="protein sequence ID" value="KAF2076666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVK4"/>
    <dbReference type="OrthoDB" id="439743at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1266" mass="145919" checksum="0C8660655335C51A" modified="2022-05-25" version="1">MIVALLYFIYNYIKSLFFKSQIKVPSEFPREFEKKFMRWENKRGEIEYIGIYICEEKSNGWYPSHPLPVEITSLCIVVCRNRDGTPNVDLLPPIPEQINRLALYFESAYSNLKNTYTFNHYSLPRDYIPKTVTDLSIEILTSNLYDIFIGDYIPSSVATLSVPSVFPEMVKYIPSSVHHLTVKKQRSSVDNLIPSFIESLDITFTKYDGQEKSIPNTVKTLSINGIVKRQEKECPTIYTKTTDQQEIPANTLSLHWVNDIAIRSLEIPTSVTHIEFYDFNKPISQDQLPDSITSIKFKSYNQSLSLINFPTNLRYLALVDFDQMIYGNTQIPMSTVYLEVRITRKNGYSNNWLLKRGYIPESIRFLKINIGETFLFENIIVPSSIQFVDIDPFYKNRVIFLEKDIYYKDPIASNELNNGGAQHVFCNKLVSLDLGDCFNQQIKMEMLPPTLTQLYYGRDYTQEVVVPSSVTDLSLMSLQNQIKISPGSILPKIKHLTTRLPLYQSTDFSSILDPTTTVLDCFDVDFLQDKTFELTKPLPNTRKLILRLNRFNNVFESLSNQQFFDSTMVEDLSIENESNIYGQPNHLIETFPNLTMLSLHYNGRSIELSNLSSSIKQLKIMTKDPVSPDEIPGFVQDLSLYLDDSNYQYFLINQDKDNNETIEPQTDKPLHFITINSIDNESFFKIYRNISLKRMIQNFIDDMAPSFINFNYHESRSEILSRANNYDNLIIKTKNTIVSTPNIKGLYLSDKVNDLSEIHPNTTTLGCHYDLSIIVPPWITKLYIFSIESIDKVDWIPPSVTYLGLKKANQEPFKIPDTVTTLHLHSESQELLKVIPASVRILKLDFCLTLSFVFQLPKTIEKLIFKEFHLIDTKIPKHPLISNHQIYNPSKPVSKKITHLVWNCSKDVERDIIPNNVHTIIFCHSFDNHIHHLPDSVTDLRFYGRFDQHLSNVYFPPNLKFLTFGSQFTQPIYKPLPCGLKYLCIGNIPSYNNIPETVSLLKITSFRKSFSLPPPPPHIRYLSFNEHVDIAFNEIPKTCKSIVCSTDKWTAYNDNVLAQDQNTHFSQSQLHLYTKYCFNSLIKPHTLIKGIKSIVFDKYYNQILYPHSIPDTVDTVVFGREYNQVINSGVLPDSITHLEFGVNFKHNLSKQLLPAYLKTLIIKRGYYHDDLLQWLPASVQELELGDELLSNRKLFPIDKVPSHITSLILNESEKILSPSMVPPTITNLKLCKCINYDGKLPLTITSLSLSQLFNSPLESILTLSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVN2</accession>
    <name>A0A8J4UVN2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009845</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000827">
        <property type="protein sequence ID" value="KAF2068835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVN2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="156" mass="17441" checksum="3D811665744801D2" modified="2022-05-25" version="1" fragment="single">ALESGNTTVTNSEYVKLQVDDHSLYGRFIKRGIIDGRISTITNQLLPNYNHGESNQFNNIQSYIGIGIRSYKRLVQLDPDFSVLVDQRPAEANTDNSICFSSKSKRKLSGAQIAGIVIGCIAFVAIAVVCVSYYIYKKKKALKFNKNVENKLKNMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UW06</accession>
    <name>A0A8J4UW06_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_009457</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000713">
        <property type="protein sequence ID" value="KAF2069225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UW06"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044772">
        <property type="term" value="P:mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000001">
        <property type="entry name" value="G2/mitotic-specific cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 4" id="KW-0195">Cyclin</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="524"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="7">
        <location>
            <begin position="397"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="8">
        <location>
            <begin position="490"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="7">
        <location>
            <begin position="494"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="140"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="140"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2069225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="606" mass="68564" checksum="8960CD04463454E2" modified="2022-05-25" version="1">MSLQENSFVVQPQQQDQQHPEKRSSFKRCSSTDYDETSNKRALVDVTNTQNSNVVFPNTAAIDAKVVVKKNTINKENVNSSSKSTLVAPAVQDNRTFNNILNLLPNNNKQSNIIINSNITFNRKDESALVNSFNNFNKSINNSNNNNNNISNNNNNNSNNIKSNNNLNRLNQNSNQFTIPKNVNDQNNINNNFKSTNTPKPTSSPKPTLSSSPSSLSSLSTTTTTTTTTTTTTTTTTTNSNSSSSNTFVIHKCKPSPQKLIKKFTKPTPYPLTESQVISITNLQNYNNSISNNSNNNKNNSGLVLPNCFQSNKDRLATTDHDKQWCENIIDHLQGDFSSIPHFDIDTFPNPPGNDYCPEYAADIYKNALKSQVPNRPLDYMPSQPDLKSSMRALLLDWIVDIANDLNVKNETVFLTANFLDRYLSYDLVPRDKLQLVGATAFLLACKYEEYRSPSIDEIVSLSDFCFSNEEVLERESRMLKVLNFSLCVPTVKFFMSRYLRAACADPVVSYVAHFFSELTMLEYRLVTFFPSIVSAACIFLALKTYGLPWTSTLHYYTRVSVNDLFFQRCVQLVWETYITNDEKLQSIRQKYPELNCSQLQNLTFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWK9</accession>
    <name>A0A8J4UWK9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Saposin B-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_001028</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000023">
        <property type="protein sequence ID" value="KAF2077641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWK9"/>
    <dbReference type="OrthoDB" id="69496at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.225.10">
        <property type="entry name" value="Saposin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007856">
        <property type="entry name" value="SapB_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011001">
        <property type="entry name" value="Saposin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008139">
        <property type="entry name" value="SaposinB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051428">
        <property type="entry name" value="Sphingo_Act-Surfact_Prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11480:SF53">
        <property type="entry name" value="SAPOSIN B DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11480">
        <property type="entry name" value="SAPOSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05184">
        <property type="entry name" value="SapB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00741">
        <property type="entry name" value="SapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47862">
        <property type="entry name" value="Saposin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50015">
        <property type="entry name" value="SAP_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035310585" description="Saposin B-type domain-containing protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="4">
        <location>
            <begin position="33"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="141"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="143"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2077641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="174" mass="19290" checksum="00805267D476E3F8" modified="2022-05-25" version="1">MIKVLLSLFAVVLLCSSVVRAQEPDLVGSVDPAVVECDICELAVSFGEYLIKNQNFTKAQVQSEIQSVCSYIPKNYTSECKMFMFFAAPIITEALFKGESPQQLCATYKLCPKPTPAPSKVDDTPAYQDFRIVIQDAHSLLTKSREPVGRREPGGRREPGGRREPRGSRLGGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UX25</accession>
    <name>A0A8J4UX25_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008281</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000497">
        <property type="protein sequence ID" value="KAF2070395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UX25"/>
    <dbReference type="OrthoDB" id="21631at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="390"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="390"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="421"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="459"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="497"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="526"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="646" mass="75917" checksum="0E894A43D64B4F9E" modified="2022-05-25" version="1" fragment="single">EKQRLEEEEKERQRVAAELAEKQRIEQERIAAELAEKQRLEDEEKERQRLAAELAEKQRLEDEEKERQRVAAELAEKLRIEQERIAAELAEKQRLEEEEKERQRVANELAEKQRIEDEERKKQEEESEKQRLADLAEKQRLEEEEKEKQRLEAEKKRLEDEENERQRVAAELAEKQRLEEEEKEKQRLADELAEKLRIEQEEKEKQRLQDEENERQRVAAELAEKQRLEQERIAAELAEKQRLEDEEKERQRVAAELAEKQRLEEEKEKQRLANELAERQRIEDEEKERQRVAAELAEKQRIEDEKERLRLEAEKQRLEDEEKERQRLEAELKEKQRIEDEEKEKQRLAVELLEKQRLEDEEREKQRLAAELAEKQRIEQERIAAELAEKQRLEDEEKQRLAAAEKEKHHKQKEVVKVFYGEEEEDDEDQDDDTSSITTKGGITPDIKDKSEADDHSESTNTDVSISSSSRAESASASRKVSFSSLLPWNRKDSKPTTTTTTTTTSPTSSTASTNTHNPSIPSLKLNTATMTSSPTTTNMTLTEPSSPRDQKEFMSPFQASAFIELLDPVPKEKNLFNKNTDITRNVQKKWVELFGSLKKELQGSSKIEASVDSTMQALQDISKQRKCINIDMNNLVNSFCTSPFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXL8</accession>
    <name>A0A8J4UXL8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal protein S18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_000017</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000001">
        <property type="protein sequence ID" value="KAF2078646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXL8"/>
    <dbReference type="OrthoDB" id="21463at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.640.10">
        <property type="entry name" value="Ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001648">
        <property type="entry name" value="Ribosomal_bS18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036870">
        <property type="entry name" value="Ribosomal_bS18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13479">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13479:SF40">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS18M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01084">
        <property type="entry name" value="Ribosomal_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46911">
        <property type="entry name" value="Ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="287"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="297"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="334"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="372"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2078646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="669" mass="77921" checksum="39F9BD6AD9FC8AEC" modified="2022-05-25" version="1">MQSLLSKVRIVASPASTIAGRHSLRFYSSGNNKTPSFEEFKANRQKIQQKNELAKQLTQAKQSGNVQLAIDIENQIKSLGEINRTIENFAVKSKQQKESELEKWKEFLEIKGVKSKQELAKINKFEFERFKLSGLTDAEMLEGKDKQYQTTNIDLLPAGEDITIGNKKLDSYWLEKKAREGDLVELMLENLNKIEENPVDKEMFNQLIFQEEDNEDGLDLKLPKEIRDRIKALDAENPVSKEEILLNQQKLLKLVHDQTLRQDGINPDQGRESNAIINNIQVIRSQIKQQESQGEALDIEDEEDEQNNELEEELDENDEDEDEDEDELSKNDEEKEEEDEDEDEDDEDEDEDEDEDEFDDDEERMEEEAEKEEFATKENKKEKMFLQDLALSGQYRELRNKLNNNTKPDPLPEIESIILGKELKQQFDETVEKMTQDTFELQDKNHLRNFAKYSFDLQEEEAQKAKKKQQDDYNNSISAIEANINSLLNIEPPQEIVVPSTGKPFRPNHKHPNCLLCQDPHWRVEPINIPFLKLYLNESGDIIPKYYSGNCSKHQKKIARTIKHAKALGLISYKKGTFTITDPNEYPALPSEMAAYEKYFYGNYTEKEWEDLVDEVEERELLNQAAKEDDHDVPDDLVEGERYEKENVMLEFGLEVDDIDNLYDRKSNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXV1</accession>
    <name>A0A8J4UXV1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cell division cycle protein 48</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_007713</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000427">
        <property type="protein sequence ID" value="KAF2070963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXV1"/>
    <dbReference type="OrthoDB" id="27435at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051228">
        <property type="term" value="P:mitotic spindle disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19519">
        <property type="entry name" value="RecA-like_CDC48_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19528">
        <property type="entry name" value="RecA-like_CDC48_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000004">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.10:FF:000001">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000003">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000048">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005938">
        <property type="entry name" value="AAA_ATPase_CDC48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01243">
        <property type="entry name" value="CDC48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="6">
        <location>
            <begin position="23"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="CDC48" evidence="5">
        <location>
            <begin position="123"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="234"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="507"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="705"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="766"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="773"/>
            <end position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2070963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="793" mass="88643" checksum="6FC54628D38FC6E2" modified="2022-05-25" version="1">MASIEANDTTNIPILERKKAPNRLFVEEALNDDNSVVTLHPDTMDQLQFFRGDTLLIKGKKRRDTVCIVLSDPTIDPTKIRMNKVVRNNLRVRLGDMVSVHQCSDVKYGKRIHVLPIDDTIEGLSGNLFDLFLKPYFLEAYRPVRKGDLFLVRGGMRAVEFKVVETDPAEYCIVAPETFIHCEGEPVHREDEDRLDEVGYDDIGGVRKQLGQIRELVELPLRHPQLFKNIGVKPPKGILLYGPPGCGKTMIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPSIIFIDEIDSIAPKREKTQGEVERRIVSQLLTLMDGLKSRAHVIVMGATNRPNSIDPALRRFGRFDREIDITIPDSTGRLEIMRIHTKNMKLDESVDLESISNETHGYVGADLAALCTESALQCIREKMDVIDLEDDTISAEILESMSVTQDHFRTALAISNPSALRETVVEVPTTTWEDIGGLEGVKRELRETVQYPVEHPEKFRKFGMQPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVRELFDKARQAAPCVLFFDELDSIARARGSSQGDAGGAGDRVINQILTEMDGMNAKKNVFVIGATNRPDIIDPAILRPGRLDQLIYIPLPDLPSRVAILKANLNKSPIAPDVDLEFLAQKTHGFSGADLTAICQRACKLAIRESIEKDIESTRRRQNGDAMEDDGTSDPVPEITREHFEESMKFARRSVSDNDIRKYEMFAQTLVQSRGLGNNFKFPDSQQGGEQQFNQDEGDDLFRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYP1</accession>
    <name>A0A8J4UYP1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005404</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000215">
        <property type="protein sequence ID" value="KAF2073275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYP1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32142:SF55">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32142">
        <property type="entry name" value="B BOX-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="805" mass="94400" checksum="4365E1F2D02A7977" modified="2022-05-25" version="1">MIISLYRDVFRNLFLSTKIFNTISEIQRDRYSLKYNDIVDLGWMIRNGHVALAKEKLNGNRELYISPKDIFDVVADKYPEMFVVIFERFKYIALEYYQCNPLGIIEQQKNIDIVRYLYENGYGPVELHLEICEMDVGVVEWLVVNGWLKITPKLLAASTFCMKLDQENYRYEKEMNELIVKHIEKPISVDGAKCLILGLFRNPYSGLLSSVLPLFDPRVKDEVSTEGLSFGEIIKLILDSDRKEAERDAFDSVQHRQDIRFKDPTLEHLYHRWHAWVGQGDHAFYELWETYADRITVTPDRLLFFMTVQDVDCRTQDHLLPIGGYLQTLLCLTANIKLIEFLWSQGINFTVPAAHHEVFYQELVKKKDQEDRHLIFNFCGTAFGHVVSGKQLLHFCCVYGQVDNFHYYLPLLIDDLLEAEILEIMFTAKDRHQYSCFKVLSAYIFQTTRDTDECLNIFRSLSLVYLLDQCDKLFARVPLQKRNQLCSNLLVIAIDSEDYVGVKHIFTRYKAASFSTDVMGRLASCRNTTIIDYVYTNRNTIFLGVPPKNIELFFSKIFGMVVMTSLAGLNLMGVEYFFKNCISQSQVSPVQWSSLQPYFGQDDSHRVISRDIKNFHSLVYLLDHHNTYNSMDCFIDSLVNRTDANPLESFEDVDDLDFGQEVDSDDDDDEYYDQFNTESKQDDDFFKYEHSGCDTINLLFIEYLYVHQDRLFKVKPSFQIVFDRIVFEKKYLKKYKVVLAALINRYGCTLKDDHYHFLDRHLNGLTLFGRFPNSMFVQPNFKPPPLPLVLKDKRSFKKSKIDWEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYS3</accession>
    <name>A0A8J4UYS3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_005165</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000199">
        <property type="protein sequence ID" value="KAF2073515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYS3"/>
    <dbReference type="OrthoDB" id="14404at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF184">
        <property type="entry name" value="GTP-BINDING PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="4">
        <location>
            <begin position="117"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2073515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="417" mass="46833" checksum="EB2C36916738AE13" modified="2022-05-25" version="1">MFNINPYKKSKSPFSPSSSSKDKKKKKEDLISRLQVPSPTTENSLSLTSISEITNSLSNTHTHTDTSLTAEDASEIGEDYDPFLSILEEPGTEDRAAIEKETTEELQEMLAVLKGVQNECNVLLLGRTGVGKSSTLNTVFGIDIPVHSSESCTQDPFTYSRCVNGFKLNIIDTPGFLDSQGEAVDSNNMLKIQKYLSGKTIHCVLFVEKFTETRFDGAHQLVINQFTEKLGPQLWRNAAVVLTYANSVLPDSCFDGFDEDDEIGPWKKHYDARALQFRRFFSNILAQLPQDDYPPKHISVFAMENSRRCKRNEQGQRILIDGTPCLHLLISGLLKMVDPKTAFLFMGHLRAKKPGSKGHRGDQNDRERSIMDNVTEILKLFIVPPFDQLGKGTVAKILENWGKKLDRYGNLPNLLRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZ59</accession>
    <name>A0A8J4UZ59_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004585</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000165">
        <property type="protein sequence ID" value="KAF2074100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZ59"/>
    <dbReference type="OrthoDB" id="5854584at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039632">
        <property type="entry name" value="TMEM42"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31965">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31965:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="169" mass="18723" checksum="62E5E16F01828E4C" modified="2022-05-25" version="1">MSNKVTSRSNNENKAKENKQNNTGASTSKPTVVNTNAKMAFIFSMIGGIFASLSSVCGKIVVDSSFLSSLISQTQIDLVIRAISISGIFISTTMQWRYAAKAMDLSTNSLSSTVITTASNFFFTAFFGWLFFKETLSIVWWIGASFIMFGLFLMNLDSNQQLEKEKKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZ76</accession>
    <name>A0A8J4UZ76_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nucleoporin Nup54 alpha-helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_006095</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000266">
        <property type="protein sequence ID" value="KAF2072598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZ76"/>
    <dbReference type="OrthoDB" id="6162375at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044613">
        <property type="term" value="C:nuclear pore central transport channel"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006607">
        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036228">
        <property type="term" value="P:protein localization to nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025574">
        <property type="entry name" value="Nucleoporin_FG_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024864">
        <property type="entry name" value="Nup54/Nup57/Nup44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025712">
        <property type="entry name" value="Nup54_alpha-helical_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13000">
        <property type="entry name" value="NUCLEOPORIN P54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13000:SF0">
        <property type="entry name" value="NUCLEOPORIN P54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13634">
        <property type="entry name" value="Nucleoporin_FG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13874">
        <property type="entry name" value="Nup54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup54 alpha-helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="160"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="400"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="82"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="99"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="177"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2072598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="434" mass="48742" checksum="C9BF2E34B6D07FA9" modified="2022-05-25" version="1">MFGGGNLFGNPATSTTPSLFGTTGATATSTTPSLFGANPQQQQQQPQQQSTSLFGNPATSTTPSLFGTTTQQQQPQQQSTSLFGNTGAPASTTPSLFGTTTQQQPQQQSTSLFGNTTQQPQQQSTSLFGTTTQQPQQQSTSLFGTTGSLATTSSLGSLGTTASSSLFQPSTSNSFLQQQQQQQQQQQQRQSLSNIEQKLFDVIQSVSPDNINCRFLYWYYNYQQGPRVDPNSIPKPPNVTVDQWTYALSNNPDPNCYIPVPAKSFEDLVNRRKRQQETLDFLHQNTQQILTSMRDFQNYLNFQIHSQLELMRKKQIELLHRYIEVWSNLEIHKSKGKPFTITEEMISKKIQWLFEQINQPNSIKSKVEEIINQVKVGAMEKEKIKYELSAEAIESLYLLLKNMTNSIERIANELEIAVKDAQILKNELGHFKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0C9</accession>
    <name>A0A8J4V0C9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RED-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_004411</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000155">
        <property type="protein sequence ID" value="KAF2074278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0C9"/>
    <dbReference type="OrthoDB" id="3366823at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039896">
        <property type="entry name" value="Red-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012916">
        <property type="entry name" value="RED_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12765">
        <property type="entry name" value="RED PROTEIN IK FACTOR CYTOKINE IK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07808">
        <property type="entry name" value="RED_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RED-like N-terminal" evidence="4">
        <location>
            <begin position="80"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="202"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="295"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="27"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="83"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="303"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="375"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="396"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="417"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2074278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="529" mass="60876" checksum="DDBB0D11F0F582E1" modified="2022-05-25" version="1">MSTLKQDSSSSSNNNNDRNSKKKKKWVERDYNYYKNAPDKIDDPNYRDRAKERRNQNEDDEGQSTKSSGLDFNLLKRDDSHLNSSNTSSSSSGGNKETKKTITCKSVLGNSIYNVLLSSATNSNQQQQQHQKNKELFQKGRMIYVYELDPNFPSLIPTTILNSKEDCPPIKHVISGKLDTSILKKVIESYNPDKTVNIIKEKNNSDDSHNENNSPSNNNNNNNSLNILLPTNNDDDDSGSDIFSDAEEYVCDVVSKVNKQMPIDDNEEADKKFDYFSKSNTGVDSKEDEMMKEFNPIGKEGDEKEDGEDGEIEGPSMPPHDDQDDEIEGPSMPPQDDQDDEIEGPSMPQDNASDQDEDQDIGPSIPQHMYINNNEDQEDEEAYPDTDMNYERPNNEEEEQEENSQDEYEYPNTEDNFNQHEEKQKIEMEKRYQDNSDSDSDDLPIIDTNNESLKRKLLEQQDSYGDVIPSYQGFDRERGEKPQHTIDEMYKFKKEPKKKRSKTIDGEMHKVNSMLKKNYGDKYDLFNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0M3</accession>
    <name>A0A8J4V0M3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Methyltransferase</fullName>
            <ecNumber evidence="13">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_002778</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Steroid biosynthesis; sterol biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11 12 13">Belongs to the class I-like SAM-binding methyltransferase superfamily. Erg6/SMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="13"/>
    <dbReference type="EMBL" id="AJWJ01000080">
        <property type="protein sequence ID" value="KAF2075930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0M3"/>
    <dbReference type="OrthoDB" id="4310724at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003838">
        <property type="term" value="F:sterol 24-C-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000168">
        <property type="entry name" value="Methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050447">
        <property type="entry name" value="Erg6_SMT_methyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030384">
        <property type="entry name" value="MeTrfase_SMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013705">
        <property type="entry name" value="Sterol_MeTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44068:SF1">
        <property type="entry name" value="HYPOTHETICAL LOC100005854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44068">
        <property type="entry name" value="ZGC:194242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08498">
        <property type="entry name" value="Sterol_MT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51685">
        <property type="entry name" value="SAM_MT_ERG6_SMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12 13" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="7" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="9" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase Erg6/SMT-type" evidence="14">
        <location>
            <begin position="65"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2075930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="354" mass="39866" checksum="9E14DC23E90E9B05" modified="2022-05-25" version="1">MNTQQRAMEGDLSRIIAVARKERDAVNRDNKVQESLDQYRELFKGDDKKAIEARKNNYTSMVNQFYDLATDFYEFGWGQSFHFATRHKYESFEASIARHEMFMAHQLGLFPGMKVLDIGCGVGGPMRTIARFSGAHVVGLNNNEYQIQRGKRLNEMAGLSHITSFIKADFMKVPQEAASYDCAYQIEATCHAPDLVGCYKEVYRLLKPGGLFGGYEWIMTNKFNKDDPSEVKIKKEIELGNGLPDLVQPAVIVSALKEAGFEVITAYDVAETSDLPWYLPLSGSFSITGFLHTGVGRYLTGKFTQLLEVFKLAPSGSYSTNVWLQNAATYLVQGGEKQIFSPMFFILARKPEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0Q3</accession>
    <name>A0A8J4V0Q3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CobW C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_002594</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SIMIBI class G3E GTPase family. ZNG1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000073">
        <property type="protein sequence ID" value="KAF2076080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0Q3"/>
    <dbReference type="OrthoDB" id="272672at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03112">
        <property type="entry name" value="CobW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1220.10">
        <property type="entry name" value="CobW-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036627">
        <property type="entry name" value="CobW-likC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011629">
        <property type="entry name" value="CobW-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051927">
        <property type="entry name" value="Zn_Chap_cDPG_Synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43603">
        <property type="entry name" value="COBW DOMAIN-CONTAINING PROTEIN DDB_G0274527"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43603:SF1">
        <property type="entry name" value="ZINC-REGULATED GTPASE METALLOPROTEIN ACTIVATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07683">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00833">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobW C-terminal" evidence="7">
        <location>
            <begin position="304"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="446"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="446"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="497"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2076080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="518" mass="58810" checksum="AA43F2039EF02066" modified="2022-05-25" version="1">MSKNNISMSNKGKGKVIKQTTSQIQRSKNLKLAKAIRNVRARHVRKLPVTVLSGFLGSGKTTLLNYILNNQDGMKVAVIVNDMSEVNVDARLVKSDFSISRTTPKEKTVEKMIELSNGCVCCTLREDLLVEVAKLAKEGRFDYLLIEGSGISEPLPIAETFTFDGEEGMNLHHMTRLDTMVTVVDCSTWLKEYNSGKSLKDKDMGATDTDERTIVDLLIDQVEFANVVILNKIDKASPQDINIMEGLIRHINPDAKILRSEYSKVPLTEILNTKLFNFKKASEHPGWLQEARGAHIPETIEYGISNFTYRARRPMHPGRLDDLIAKGATVFAKVLRSKGFMWVATTPELIGLWNLAGTALSLTNAGYWLASLKKFEYPDKETVKMIKKSWQEPYGDCRQEIVFIGCEMDQSLIEKELNDCLLTDEELKLGKDVWATWEDPIALDEEVEIEVDDEDDEDDDDDDDDEDDEETTVEEEDDDNEMDQEQDEQNEKELIIKTKHNHNHNHNHKKPSISKDIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1J1</accession>
    <name>A0A8J4V1J1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001577</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000038">
        <property type="protein sequence ID" value="KAF2077130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1J1"/>
    <dbReference type="OrthoDB" id="10656429at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="276" mass="32117" checksum="CA8294D5BAE7AB96" modified="2022-05-25" version="1">MDQPKQINREPINRLIKYYVIVVRSILCVLFCYYLKSESNNELLASIRQSFTPYQIYLIVSFVSTLLFLITVYTILGPSYKDKTKQEMDAMSPPKRFIFTNFFKGRRAMAFEILVLFWSFSILYMTLNNNFTFNKYTQYERLKKVIIFSLGSVIALGYLLVNIRNRIENLIPQSTIFVNGGGSSGSNSVYNSPLKALSDTFDKLLNITLIHYAITSLFYMVLSYLTTFWTTPSNQYTNTLIFIEVPFTVCSVYLTNKLYNLNKNYQISTLKKKKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1J8</accession>
    <name>A0A8J4V1J8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zinc finger CCCH domain-containing protein 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_008165</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZC3H14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000481">
        <property type="protein sequence ID" value="KAF2070517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1J8"/>
    <dbReference type="OrthoDB" id="5589010at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043488">
        <property type="term" value="P:regulation of mRNA stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.40:FF:000006">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.40">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.40">
        <property type="entry name" value="Nuclear abundant poly(A) RNA-bind protein 2, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040366">
        <property type="entry name" value="Nab2/ZC3H14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043094">
        <property type="entry name" value="Nab2/ZC3H14_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002483">
        <property type="entry name" value="PWI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036483">
        <property type="entry name" value="PWI_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14738">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14738:SF29">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01480">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14608">
        <property type="entry name" value="zf-CCCH_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101233">
        <property type="entry name" value="PWI domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="245"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="328"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="245"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="328"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="80"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="159"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="169"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="184"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="411"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2070517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="452" mass="51076" checksum="D53C29DC7AF152DB" modified="2022-05-25" version="1">METLGNYSYTGEHVTFIQNQVKQYLDCDDILTEYISVMVRNNKTKSEIINDLSDFIGSEKATSFVGWLWNFIPSYFASATSNSNNTTTTSKPVSPTNESQESKKPTSRLILNAVKEASEDTKNIKLQSESTEMEQEEDTEQEHTNGNSGSVFKKKFNRDNNNNNNNNNRDNHRDNNNRDKRNRNNNNNNNNGRDPTFTITFNSDSYSKKRKNNSRDDDPLEHSLDEEIELMNGNNNNNNGTPSKKSKKERCQFWPLCKNGDACPYHHPTTQCMLFPNCQFGNKCLYIHPSIPCKFGVNCTNENCVYNHPQRSMMPAAEANCRYGFSCPNKKTGCSFYHPPLACKFGEKCTNGRSCPYGHGKPCLFGASCVTPGCYFAHHLTESAFPECKFGKECTNPKCKFTHSDRENTEDLSNTLPATPTSMETRDDLTVSMEAIDNIPQDSTSEAVTTEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V220</accession>
    <name>A0A8J4V220_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007648</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000420">
        <property type="protein sequence ID" value="KAF2071027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V220"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF180">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="420" mass="48250" checksum="CF5A127466B263B0" modified="2022-05-25" version="1">MDLKSFFSDAWNLKSFSCKSGEKKIFKLGDIPQLEHLSLNQLKWGNMKTDPFPWVTLKSLKIFDRIRFIVPQSLMEISSPYKAKVYPKYVKSIVINGPDELVDIDATFPPPNVKELTITHLGNIGASYHQLDLVKFCIGMNRTPFDSDLFTSFTHLTHLEILKYHGNILSPGIFPQTITIMSIGCDFSIECGVLPKSLKRLTFTKHFNSPLIPYSIPNGVEFIKLEVSGYKESIPNNVIPLSTKELHMTSRVIKPINAPPTTKINFYLKIIYRSVPEIPIYVTKFHFATTTVPLSEIKIPCSVTHLVLSHYNLSIQSKDIPDSVTILELPKRFTYIDARSIIPKNVRYLIIHSLCNISNIPDSVEYLMVRRNRNYKSTLDAQFPRGLKKLYIANYQYYFKNVPLSTILYPVPYRMKFKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V292</accession>
    <name>A0A8J4V292_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007386</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000391">
        <property type="protein sequence ID" value="KAF2071297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V292"/>
    <dbReference type="OrthoDB" id="17470at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="226"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="158"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="400" mass="45873" checksum="0F486857027EA4D7" modified="2022-05-25" version="1">MDNGNSKPHNLTVDSKKIHEDISQSINGLHSLINGHFGSLQQDITELLKRKDVLLDVTKVIDVSMIPDPITLNIGGFKHQTTKATLTKIPNSFFDLMLSGEIDIKPMTNKPNTYFIDRDEGDIQIPEDIRHCVKKEMEFYKINDHFKLDDTTTIIDQLNSLKLKLDETEQEFNSTKQELTETKQELALSKEESKKELENLKQEVNSLKDKDKLLDQINNSKLPFSLILNQHTFKIIRNWIDKTKLTNFELLYSASKTNRFKASIFHLACDGEGPTITVIETTDGCVFGGYNSQSWNSKGFYYGDNKCFIFTLVNKHGTPPTKYRPKENLKYFVVGHSDFGPVFGNIDNNDGLEEDELVIKELDSYQRFPMQYNDTTGKGKTTLTPSEYFHIKNLEIYKCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V407</accession>
    <name>A0A8J4V407_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001560</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000038">
        <property type="protein sequence ID" value="KAF2077113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V407"/>
    <dbReference type="OrthoDB" id="17928at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010393">
        <property type="entry name" value="DUF991_YecM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37519">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37519:SF1">
        <property type="entry name" value="DIHYDROXYBIPHENYL DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06185">
        <property type="entry name" value="YecM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="200" mass="23530" checksum="9D897248054BF0B4" modified="2022-05-25" version="1">MQLKDIIGDYDTFLDILFEKILNSDGFSADEIEKYQSDHICYRVSTPELYFEKKKQLLEIGVLLSEAVINGRPIASIKLHQPIVYKGRDVQMIELPMPKPGRPHWDGLEHMEMVIDTDFNEFINKHSKNSWVLGAITKEINPDIELEFTLTEQDKLKINHPSDRLDQHRTISVKFHRQPLDKVIEYELELERKQKENENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5Y2</accession>
    <name>A0A8J4V5Y2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">CYY_000177</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000003">
        <property type="protein sequence ID" value="KAF2078553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="154395at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF221">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="243"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="453"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="14">
        <location>
            <begin position="1044"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="1507"/>
            <end position="1726"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="1511"/>
            <end position="1550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="65"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="1410"/>
            <end position="1437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="66"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF2078553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1783" mass="202000" checksum="099F704B516C9FDF" modified="2022-05-25" version="1">MSCKSFTKEIGFCPVKDCNLSHPPKCRNDNACFKNDCTFYHSVSKCQTFPCLNKKICILRHVKKNSSSTSTPTSSTSTSTSTPIPTPTPTSTSTLTSTPTSTQTRPTFFPSHNQNNNDIFQNLENEYKSSIDLYNQQLKLENEKLLIKESEIKEHQLNISNSFEVLKTLKCGIDIFKSQIKVSNDTVESISNSFKEAKSTPIMKDSIREIKNIVSKIYRETWRLKHSLPALSKRKEIEDYIRSMKTLKNNFIIIKGYTGSGKSTQVCQYIADMNEFKGKTIICTQPRKLAVLSLRDRVSMEFNAENPNTVPNEQVSILTNSSNTKDQISSRILFCTESTLISKIGDIIQEMNRNPNTNNNNNNNNWLNNNVGAIIIDEAHERSINTDLLIGLLKMNKNLWSNLPVIVTSASLDTQLFLNYLNNCPLVEIPGKMFPVQVYYNTMVSDQSIVNCVVSKALELFKFKGGDILCFLTGQEEVEKAKLKFDQLYHSSKALEKDQNNCQEPISLCLYGKQLPDDQKKVFETTPKNKRKVIFSTDVAETSITIDGLEFVIDSGFAKSNIYDPQKNTMSLKVHTISKSSADQRRGRAGRTGPGVCYRLYSSNEYDEMQEGVRAEIFCRPLSTLVATLVSMSINPLSFNFIESPSRKSIEMAICHLKLFDAIQRNVDINENTPPSIISSYLTLHIGQMVVKHQVDPGIMNMIYQSCELGFGEAATRIAGIMNVGSMILWRGNDEKSKAEASTKHSNLLSETTNLLDPSVYYKIFSDWVSIKSKEKDHESISCPKWSADNFLNNKALGIVRSNSNELISALEDYQFWKFRDQTNEPSSNDIRKLCLYSQFNNIAVQIKPGVYKTIPIGNSESSIAMLHPSSIIKRGKDSDADSLPETIFYQSILNSSKPYLSMVTSIPDLNWINQATVNGEEISYNVEKVRRESKVEQITIENVSRFILIEILGKQGSNTDIVIGKLGCESLSCRVKDESFSCLYNPYKVNSNIIRERMQDMIEQVRYKLSIEAKEEQYLGQSRVVFGSGLKVVDFLTNSNQFISIYISNLPNELINTQSISNFIIKMIKDKLDYGNIKDHELIRQVYVHPSSNEKKGWAKVMLNNSNLAKQIQKIINGEVCNGEILKCTGSGISLPSTLSSIFGTLVITLINLDGGKGKLEFERALSPSELAQVQLLPIFKDFQSNGKFTLSSISYDEDNIREYIFNEKLPQPKKIILYRRPISQEFGYNDVLDMDKIIPQSSDIDSGIPFKDLRSNTYGIKYYFNDATLLESIFQNLMRLKDPKYKINVYYNSNVSIHFQINKLNCDSIDTLSKEFKSLHYGNRFKSVTNSATTSNGNNSFEYKLSSKNINQLKNTINSFHLLCNPSDIFTHSNKELVFSTYGRKCMAKLSIQTYLYWNNKTHTIMIYSNSKQEIEDAKNQLNSLVTELENLQIDTKFTLTLKGRLELVEQDKKDSNFIIKLVDGLKDSRISGRTLFVTGTKESIDKLEMIIKDKLVPRNRNNLGTKECSICFSEDEELFTFQKCGHQFCRSCINSDMLVSNNSIQKCASIGCDAEISLCDILNICSNGEDLDIIKRRSILEYQRKNSSNVYCCPNPLCLQLLKLPTKDILLNSGDINNSFVFCDQCKLEYCIFCSKKQGKATLSHRGLACSVSASAYTNLLDLLNTKCPNCQMVFFEFDGCYAVVCTGQGQGGCQAHFCGFCLHISSDSISNHNHVQNCAQNPGVGYYADKKVFYDIRNNEKKKKIIESIKKEQDMEKRLVLFNSIKSHCEFNIYEYEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7B5</accession>
    <name>A0A8J4V7B5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cation efflux protein transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_001995</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000052">
        <property type="protein sequence ID" value="KAF2076682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7B5"/>
    <dbReference type="OrthoDB" id="78669at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904257">
        <property type="term" value="P:zinc ion import into Golgi lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045316">
        <property type="entry name" value="Msc2-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45755">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45755:SF2">
        <property type="entry name" value="ZINC TRANSPORTER PROTEIN DDB_G0269332-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="284"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="323"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="360"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="8">
        <location>
            <begin position="181"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="468"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="29"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="98"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="490"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="526"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="555"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="582"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="609"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2076682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="646" mass="75083" checksum="BF65179D6C562BDA" modified="2022-05-25" version="1">MKDNYDPYSHDHDNQNQYQQPQYNVVGGSPSTGTTIVSPQSISSPVYDYNINSSTNQYNNHGHSHNSNSGGGGHHHHNRLGHPQPYYASYNDINSKPQDNNSNNNYNNYSNYYTQQQQQQQQQYNNNYDNNNYNNNNNYYPQQQQQFSPQQFYNNNLNGGNVSFQLLLNNLLNDNDAKKLAIWIVVMLIFTLYEIFYGAYLESLGLVSDGFHTLFDCIGLIISLMGMIFGRKGPNQDYTYGYDRWEVLGTFANCCFLLFVSFFLFLESIERLLEPPHIHDHGRVISLAFVSLIINLLGLFFFKRQHSPMDSNNNRNKGLIRNMNLLTITNHILVDSCTSLGVIFSSLLGKTMGLEISDSLISLIIAAIIVYDVLPVALRTAKTLLQTTPDAIKQDIDSAMIKIQKINGVLDCNERHFWAHSSGSYIGSINVIVRSNCLEHEITSTVRKHLSFIQDLTVQVEREHIHNHNHQHSHNQEQLNHEHKHHHNHDNHDHHNEHNHDHHDHDHHEHHDEHNHDEHDHHGHSHNNNNNNHGHSHNNNNNHHGHSHNNNNDQQQEHHHSHDQEDEVPKYQVQQEDTDEIEHEHEHEHDHEHIHDHHHNENDDDHNHSHGHSHQHKHNHSHDNDHHNHNHSHNTDGWGEDDDSTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V969</accession>
    <name>A0A8J4V969_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_002912</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000086">
        <property type="protein sequence ID" value="KAF2075779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V969"/>
    <dbReference type="OrthoDB" id="1926878at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23810">
        <property type="entry name" value="UBCc_BIRC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23802">
        <property type="entry name" value="UBCc_UBE2Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000159">
        <property type="entry name" value="Putative ubiquitin-conjugating enzyme E2 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116">
        <property type="entry name" value="(E3-INDEPENDENT) E2 UBIQUITIN-CONJUGATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116:SF39">
        <property type="entry name" value="BACULOVIRAL IAP REPEAT-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="5">
        <location>
            <begin position="190"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="UBC core" evidence="5">
        <location>
            <begin position="598"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="102"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="533"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="106"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2075779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="824" mass="94772" checksum="D244FBA7A38B745C" modified="2022-05-25" version="1">MAGNLERVFEKWQTSQADPYEFVWLQSTKNSIDLLFKGHEITITFPLDINNIDEFVFSCREKTVNNWIVELNKEIKQSPPSDFNDVLSLAVKIYQRKKIKLSNEKDDSETQEGEEEEDEDDSNVDEDEEDEEDDYGDDDDDDHHDDEGFQEALQSLKLKKIWAQKDLEIRSQLGEKKKVLKVKSIFSSDAAFSILTNDLFKIMQNTQEFGFSAEPLDDNIYFWKVKLFGFDKESKIYSHLQEIQREYGYDYIEIHVMFTPDLYPFYPPTVRVIRPRLQGFLLGRISHIEILELENWNPINDMKFVLDHLKQMIQTHGLLDVNNPMNALTYNKGSYSSLEHLLLRLEILSNIAPRANLKYGIPSTFVKLKGDNKVKEGDSQAWAKGTGYGRGSDTKGWNVNAFLAAQKERDEEIVALIKSIENEVRNASIPFDVLEESCLVPFFDSYYRDVSLLDIERHVELWDALMKLTDSLLDMENYLPLFAHLEFQDKSLSDLFKDLSFQCNLILKQIDTKEKQEGYGQIVDIYQKIDRVGKKMESKLQILKEEIKKLDDMRKKTQTTSISSTDCQAMYLSKLRPLIFDTYTSTKAEAKGMPSQQSRTLRIAQEQSSLIKSLPLTFDSSVFVRVHEQNIDIMKVLITGPSDTPYSAGCFVFKVTFPANYPFAPPSVILETTGHGSVRFNPNLYNNGKVCLSLLGTWSGGAGETWNPSTSTLLQVLVSIQSLILVPEPYFNEPGYESQMSTPTGKASSKKYTEGIRESMVEWAMIDMMKNPPKEFEDVVKLHFFYQKDKIKAQLNKWVDENSSSSKLLKLLSRVQTEMDSIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V9A7</accession>
    <name>A0A8J4V9A7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA topoisomerase I</fullName>
            <ecNumber evidence="7">5.6.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">DNA topoisomerase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_000180</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6 7">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">Belongs to the type IB topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="7"/>
    <dbReference type="EMBL" id="AJWJ01000003">
        <property type="protein sequence ID" value="KAF2078557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V9A7"/>
    <dbReference type="OrthoDB" id="47179at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00659">
        <property type="entry name" value="Topo_IB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.41:FF:000001">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.10:FF:000011">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.11.10">
        <property type="entry name" value="DNA Topoisomerase I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.15.10">
        <property type="entry name" value="Topoisomerase I, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.41">
        <property type="entry name" value="Yeast DNA topoisomerase - domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013034">
        <property type="entry name" value="DNA_topo_DNA_db_N_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013030">
        <property type="entry name" value="DNA_topo_DNA_db_N_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001631">
        <property type="entry name" value="TopoI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025834">
        <property type="entry name" value="TopoI_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014711">
        <property type="entry name" value="TopoI_cat_a-hlx-sub_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014727">
        <property type="entry name" value="TopoI_cat_a/b-sub_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013500">
        <property type="entry name" value="TopoI_cat_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008336">
        <property type="entry name" value="TopoI_DNA-bd_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036202">
        <property type="entry name" value="TopoI_DNA-bd_euk_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013499">
        <property type="entry name" value="TopoI_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018521">
        <property type="entry name" value="TopoIB_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051062">
        <property type="entry name" value="Topoisomerase_IB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10290:SF3">
        <property type="entry name" value="DNA TOPOISOMERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10290">
        <property type="entry name" value="DNA TOPOISOMERASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14370">
        <property type="entry name" value="Topo_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01028">
        <property type="entry name" value="Topoisom_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02919">
        <property type="entry name" value="Topoisom_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00416">
        <property type="entry name" value="EUTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00435">
        <property type="entry name" value="TOPEUc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56741">
        <property type="entry name" value="Eukaryotic DNA topoisomerase I, N-terminal DNA-binding fragment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00176">
        <property type="entry name" value="TOPO_IB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52038">
        <property type="entry name" value="TOPO_IB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="DNA topoisomerase I eukaryotic-type" evidence="10">
        <location>
            <begin position="430"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="375"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="736"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="788"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="20"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="125"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="176"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="197"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="216"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="228"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="269"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="active site" description="O-(3'-phospho-DNA)-tyrosine intermediate" evidence="6">
        <location>
            <position position="827"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2078557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="867" mass="97118" checksum="E18693727FBA4F60" modified="2022-05-25" version="1">MEIDSTDLPITNGKRELGLEENNSSNKKLKSENGNSSNGNGNKDVTMTTASMNVSSTTTTTTTVKSEPGLPPTSSPTSTTPKKASPIVKKESTSTPTPPTRKPAVKKQESSSEDSDSSDSSSDSSSEESSSEDDSSEDEKPNKKRPAPSVSKAAPKKPAPSPAKKKLAAKKKVDSSSDSDSSSSDSDSSSDSDSDSSSDSSSSDSDSSSDSDKPKKPVTKVTKKAAATKKTTTTTTTAAKGTSTKTTTTKTTTVKKEVKTEKKKKKGEEEEEEEYRWWEEEEGDDSIKWKTLSHHGPIFPPAYQPHGIKFLYDGKPLALNPEQEEVATFYAQYLETDHVKKADFRKNFFEEFLGVLDKNQKKIVKTLEKCDFSHINEYLKNKKEVRKSRTKEEKDKETKEKKVIQDKYGFAMVDGHKQKVGNYNIEPPGLFLGRGAHPKTGKMKSRIYPSDVTINIGEDEEVPPSPIPGHDWKEVIHNNKVTWLAFWRESVNGGFKYVWLAPSSKIKGIADRKKFETARELKNTIGKIRKAYKANWTSDDNHTKQLGVALYLIDFLALRVGNEKGEDQADTVGCCSLRIEHIKFDSKKQTITLDFLGKDSMRYFNTVEINPGAYKALTEIAKGKKKGEALFDDLTVTQLNNHLKKQMTGLSAKVFRTYNASITLQNELAKMTDEYETVEEKILFYNRCNREVAILCNHQKAPPKNLAEVLGKIDDRILELNDLKDLCRIKLGKTPNSKQKDIKEREEERLKKLIATAIEKKDKDTKDKDVSDDVKASFNRSRSIPDGDDKLKKKIEKYEEQIKKLEIQKTGKDELKTVALGTSKINYLDPRITVAWCKKVGVPVEKIFSKSLRDKFPWSDVDSEYEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V9R7</accession>
    <name>A0A8J4V9R7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002299</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000062">
        <property type="protein sequence ID" value="KAF2076394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V9R7"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07093">
        <property type="entry name" value="ALDH_F8_HMSADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000001">
        <property type="entry name" value="Aldehyde dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000012">
        <property type="entry name" value="Betaine aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43720">
        <property type="entry name" value="2-AMINOMUCONIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43720:SF2">
        <property type="entry name" value="2-AMINOMUCONIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="6">
        <location>
            <begin position="27"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2076394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="494" mass="54519" checksum="9BFEF45E0408511A" modified="2022-05-25" version="1">MTEEKFMLKLNNFINGNFVSPVDDVYMNNYNPSTGEVYSMVPDSSDKDVELAVEAASKALAGWSAKSPAERSVYLYRIADLIEKHIGHLSEAESKDQGKTIKTARTVDIPRSAANFRFYAGQILYTTEPSSAHHVMGQEYLNYTIRAPVGVCGLISPWNLPLYLLTWKIAPAIALGNTCVCKPSEITPMTAFILCDILNEAGLPPGVVNMVFGTGPRAGSALVQHPKVPCISFTGGTKTGEVITKLSSPYHKKLSLELGGKNPAIVFDDCNFEECVEVLVRSSFSNQGEICLCNSRIFVQDTVYERFVEKFVERVKQLKVGDPADETCDMGALISEDHLKKIEYYVDLAKQEGGRIMCGGQRAQVGDADSKFRKGYFYQPTVIVDCKPDSRTMQEEIFGPVVGIYHFSTEQQALSYANDIQYGLSSSVWTKDLDKAHRVAAKVECGICWVNCWMVRDLKTPFGGSKASGVGREGGLHSVEFYTECKNICIKLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJC5</accession>
    <name>A0A8J4PJC5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010188</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000964">
        <property type="protein sequence ID" value="KAF2068485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJC5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="191" mass="19933" checksum="DAD54D6ECA6CA469" modified="2022-05-25" version="1" fragment="single">TGIFTITSTSSSPRIYQDPLISNPYSSIYKSSSSFSRVDSLVSNSYECTYKSNSISPSPEPSSSPSTCPTLIPSSSPTPSPSPSPSPSSSSLPTSSPSPEQSSTPSSSLTLLASSSPSSSPSPSSSVGEPSFSSESSPLELSTIESISNEASYKTLSELGSIKTEIIQESLAQSNQPSIYHLLLLFLFILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJX6</accession>
    <name>A0A8J4PJX6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010342</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001051">
        <property type="protein sequence ID" value="KAF2068332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJX6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1121"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1121" mass="125088" checksum="2C542B3A13610470" modified="2022-05-25" version="1" fragment="single">EFYGDTIYTLATLEVRNSKGALLVNRDVELSVKSKDTSILSKQISDFDFQNQREAYEAKLPTTGETSGIIVTQQPGGVYYFYCNTSITVDQLFASNGREYYPPFTVYAILGGSNVTTTSSTIKLPIVNDITFVKTPIFSLINTTVIQELGTTSCDFNVWFSAESIDDNYYHSYELSFPGTNILPALATMISRNSSFVTFKATLTINTLIDSLLVVKNIFTDQSASLKFFPQISCLEPISSNTTDFQILEFGFGKSMALFATDLSTKNASITMGDYSTINLQPISFYSSLWGKFINLKPINTGIKSNQATLSTLVIGQQMSTYNYPNIGIFKNVAAGELNISPIFKPVPTILFAESSDPLFNNNYYIEFSFSLLIQNSIASIDYYIDGQHVFKDREIYGTSYSQYIECPYTANSLTVEFNFVNSTGSSWSATSVGPAIGANIFNYQITSIPSIDSLNNNNIMYGPNISFDGSFGYKPKTLLSLSRFTFLKNIIYSFQDFYFYFPYRFSGNLNVISSSSKISLAQTNPSTFQVLYQDSNYTTTLNSYNYNILVDTFNPLVKIQLDFNDKLDTILFNIQDQLGLNSTSCKASLANIEFNLDTNSFYYGTNINSTNYLFQFKLNRYFCNTPLQLSCCDILNHCIKSSVDIDNDQMIVPTTNRCNKNYQGDPKIVFIDYYFNNHSSVSFMIGVGNLGTANDYSISLILSNSIDNNDNFKTSLNLQPTFDQHSTNENSLGVYFYKATYFLDTITTSTRKYISLNLIDNQSKTSIHLSTNELDFISKSLNANFPTSLIFRNSNYLTPKSIISNILLVNDIASNSINITNTNWSNINNLVVCILGGHMTDLCYPFTLSGLATTKNNVISRLDDNVIRVGYNHIPKVSLLVYIKNICDQFSNCLSFSANDKNMSIVASNLGPTSSSNNQITLSKFTPSQISLDTTSRDRSITFNVDISSSATLSSVILPMLFIKQLVTNDILNCTLKYINVNNNTWSYQGSIQFPLAWGIHGIDEISVWNVYNSNGYFDGLLIPSSKFSIKQVDLSKPSIYQDSLVYQFNTKNLLFSGFNIGLLIKSPAFTPVVFGTTQTNIQVLNNTALSMDLSSYTVIGPFTMSIGGVVYTINEFSNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKA7</accession>
    <name>A0A8J4PKA7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ADP-ribosylation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_010300</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">GTP-binding protein that may be involved in protein trafficking. May modulate vesicle budding and uncoating within the Golgi apparatus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001025">
        <property type="protein sequence ID" value="KAF2068373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKA7"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000412">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 9 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="33"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="135"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2068373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="21738" checksum="7601CA7471C4806D" modified="2022-05-25" version="1">MSLQYIFNLVGDFINKIYSLFEGTKDAKIAMIGLDNAGKTTLLYKLKLGEVVTTIPTIGFNVETVEYKNLNFTVFDVGGQERLRVLWRHYFEGVKAIIYLIDSSDLERVNETKEELMKVLENDTLRDATLLIYANKQDNPHALTVSQLADRLDLHSIKNRKWFIQPAIATQGIGMYEGLEWLSKSLKESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKG7</accession>
    <name>A0A8J4PKG7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000885">
        <property type="protein sequence ID" value="KAF2068663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKG7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053370">
        <property type="entry name" value="QS_Complex_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35035">
        <property type="entry name" value="DISCOIDIN-INDUCING COMPLEX SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35035:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035303227" evidence="1">
        <location>
            <begin position="22"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="675" mass="73714" checksum="DDC4CD50E35136BF" modified="2022-05-25" version="1" fragment="single">MNKQVLIVFLFLCIFNYYSLAENKQWITPSIGYYHDGSNWNTPGVPLETDRAFIQIDAMVLTDAEIKVGTIGITHPDAILWADYNVNVTEVIILSEGSLIFASNATYAAGLGNRGGLVSFADAHYIGVTVNENFVSIKDVGPTFYSQFQNHYYTSIESTIDKITSFGGFFYSNDTFVVNNTNTAFYSYSAFENGYSEFKNSAMFFLDKVICNNAIFEIENSESHYFYQLTLSSSSLLNSLNSSYSFELGQLLVNDSTVRFTNDIVNVKDSLFGILGTGEVIAKNSNLNLLNSSVVIGGAGSLECSENSNLDIKNGTAYTIDQAEIEINHSNFKVDVFENEGFVLLGNSSFLIKNESTFLIDGVLSALNYSRIDIVNSQFTTIGHVLVYQNAKFLVFDCFKLNFIKYILGLADNAVFQVEKSKLQVDGLITLNDNSRLALYESELIIPGDITIQNESTLYSENSNVLVQGSILSESMILSNNSIFNIQGSLGTRSGITSFHNSTVNVNGNLLVLGGVLLSTTSNVTVQNNVTVVLGLVLAQDGSFIIENGSLNMVQGILHLNNTQFKNSHGVVYAADIEIYAGTTFVNDGKIVLSSNIMPSNESDPSSTDFKLENIGEFIIEAPSSNISVPMVNSGGSVDLKNNTVYIKQYSQNQGKLLLKGGFINSDTDIVVSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKG8</accession>
    <name>A0A8J4PKG8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010555</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001192">
        <property type="protein sequence ID" value="KAF2068119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKG8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550:SF10">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="619" mass="73679" checksum="7DF036D4244E0115" modified="2022-05-25" version="1" fragment="single">FDITWFDNSVSYANKQFFRIWNIFKSRDQIIVLPNEIQFYPNSVERSEPLNPKYGWYRTDIYVIELLVQQGYSADILKKYAEYGCEQDLYYSVGTIYSSLNDWKVVERLIKIGILSLSFMISRSIAFGHTQLFKLLYPLQDKVVKDKDYIIDAIWRQHIEILDFIGYDLNSSTHWFRNGDFTQKWCELSFLKDTYKSIQQHPDADTIATKILLNQIEYRRIENIKFMLSVFKFESFEKVLPAVLKRHDKTIIELFIQQRDIAFKSLPTNWETILESALCRYPSKNNVKLAECLIRYNLVSCPIKNHQIIDTVDPYIQHIAEERSLEYLVHNQHKFKTNPTNTQYIIDQILKRRPPVSTLLQLYVHQHFIELVFNNVFLSQKIFAFVYKIQSYSRSLRYDDIIEIDWMVSYDHLALLKEKIDRGSTLYLGYNGNELINKDFELFKRVFEEYKYFRNRHPNNRELYNIFLTNNFEAIKWFCENGYASELNLRMVKFSKDSDPRILRYLIENRWATVDYNTTFVEVSHSVFTPEILEVLKQHTPSPITSEQAKEAINCLLGNPIPLVFEYLSPLFQENIDPFEVSRACNSSHLSNYPIVEYLIENKLCKDESFIKPIYDKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLQ9</accession>
    <name>A0A8J4PLQ9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008380</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000511">
        <property type="protein sequence ID" value="KAF2070300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLQ9"/>
    <dbReference type="OrthoDB" id="10623759at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="141" mass="16700" checksum="7133DB7CB47E2B72" modified="2022-05-25" version="1">MSEINKVKKRTIIDNDDNDDNVRDNNSVSTKDIDNRDDDQDYKEFLKQKRKKEKEEQQRKFEQEQEDNIPLWKKIFYVVIRYAAIMIVFFILQKLLTGRGPFDLVTLSADESLIQKYSSYCPPGSQSCRDIIRDKLNGKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PME2</accession>
    <name>A0A8J4PME2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AP-5 complex subunit beta-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_009733</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000791">
        <property type="protein sequence ID" value="KAF2068946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PME2"/>
    <dbReference type="OrthoDB" id="646197at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030119">
        <property type="term" value="C:AP-type membrane coat adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038741">
        <property type="entry name" value="AP5B1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048981">
        <property type="entry name" value="AP5B1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048979">
        <property type="entry name" value="AP5B1_middle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34033">
        <property type="entry name" value="AP-5 COMPLEX SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34033:SF1">
        <property type="entry name" value="AP-5 COMPLEX SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21590">
        <property type="entry name" value="AP5B1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21588">
        <property type="entry name" value="AP5B1_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP5B1 middle" evidence="2">
        <location>
            <begin position="415"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="domain" description="AP5B1 C-terminal" evidence="3">
        <location>
            <begin position="1033"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2068946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1139" mass="129607" checksum="9EBDBE2E3740AC04" modified="2022-05-25" version="1">MDNQGWSSSEWYNVIQNWKNQKSFISSNTNTSTTGSNVNLSISGTTGDNDRSFGRSNSSLSMSGLGNDDDVSASIDSFKSTRYSSGGGGINTSGSGGGTSNITSSLSGSSNNNTSGNSSIEPPHVVVETLVKSLKTVSDDILRCNLLIFLQENSSLLFSLEEDPKRFERVFTILQSMINSQTDSYPLKCQVLSTMASILICESIIKTNPKLVESFIDLLLDIISKVNNSPDRLLRGSACLCLIEFELTYPCILSSYTPLFTQYCQAENSHLIQSYTNLLSTTLQNTTLNLYEDATVTYLKSNQNITSPTQSIPVQQQQPQQQQQQSSYSSSRNISSPQNSLNYGVRQLTKQTSTINIFEQSGSGEGGATVAPYSIFSIPPNIKYIPISLPKLNQMVLSNQGNVRLSESTEREVFKCASAIADQSAYLNSWGLLTIIHQLTQLIDVVNIPHNFFRNHLHLLYKFFFTDHTTLFHIILYLNIKFPDLYSQDELDYCFKRIIYLINDTQLSLENRVLAMDWFLSLPEALNQKSINIYNQYPLFYPNCFDSILLKESKLYVLSKCLASSPNQSPPDDLLKSLNCLEEFKYHSEQSIVPRIVFSAILEYLTTFPILLFSKVEQFLSDILIQYPQFLNIIISLLNGLQQGNAFCKKTKINIFLSLSKIIVLLKPLDFLRYLPLVERTILENQIDPTPLLYKIYDLIRKKAICLNGNWYIGNIILSICRNTMIYHHIPTIAKPVKIILSFMSSYFCNLEIRDRSSFYHRLISHIPDDKIKALLNTQSLQNYESNVSGLVSTPIPSDKYKTITSVSNFLSISQLDKPVVNSFDIFEPNDVTKNSISSNDDHDIDYLNYISVIREPNRKLNATITIPFQIKYKRNISQSNTPLKVYALLIQLAQSSYYSHIHPIRIPYLCYPEANTNLILSNSNTNTSDPSYLLMEKINITSNNNQDEKEKSSLMDQQKFKEFPYSYDIDISFKPIYPIPVSFIVKTTFNDDEGKTCKTEGTSISIQFHDLFMKIPIPLNLKSSPNQFLSNLYHRLWNQNPKNNTMIESVKRISKTREQIQNALQNHKLTKFLIVEQEKEEQESTNENNVSKIIAFLPPQYHLLFKIEIDKDSAIVHIKTDYWRCLSLIDSYLSTIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN68</accession>
    <name>A0A8J4PN68_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Beta-lactamase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009216</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000656">
        <property type="protein sequence ID" value="KAF2069471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN68"/>
    <dbReference type="OrthoDB" id="31129at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051478">
        <property type="entry name" value="Beta-lactamase-like_AB/R"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22935:SF94">
        <property type="entry name" value="BETA-LACTAMASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22935">
        <property type="entry name" value="PENICILLIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035226315" description="Beta-lactamase-related domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-lactamase-related" evidence="2">
        <location>
            <begin position="46"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="530" mass="60234" checksum="DA545031C6D0B0C2" modified="2022-05-25" version="1">MKNQLFTILILLLIVNVNLILSCPEYPTPIDIDQNDPLLVKAYAQVDELIQKKMKENRILSFIATIVYQDQIVWSKTYGNVDPLDPKSPPLTLDNNVRIASITKVFTDLMMYQLRDKGVISLDDPISKYYPEFSVRDLYNTKREISFRELASHQSGLPREVPCDFNLLPTDNCTEEVIIENLSKTFLILPQYKQTHYSNLGIALLGRTLAKAAKTNYEKYIFEKILEPLGMLNSSFSYEKVKDHMAIGVDLYPNGTYTHAGILELGWGTPMGGLFSTARDMSKFMSFWMNENPDVLDSSSVQEALSPIALVNDGQTAYGTPFEMYYDSVNSIWMKSKAGALDGYRSQMALVRPLKLGMFFSSLMYISSPDVFTRDAMKIIIPVYEALLLEKNSKPQNMNPEEGDQHKRHHPDQIPDQELVGQYADNEGGVFVVDNSTGYLLANFGDNNNYNVSHFSFDFPHVKRIQLVNTDPYECVFVEDGSNYELVYFNFGEKQSVQVMGQTMYLISKNPNDPLPSTSLNLKPNKRLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNH0</accession>
    <name>A0A8J4PNH0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008378</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000510">
        <property type="protein sequence ID" value="KAF2070310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNH0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="181" mass="21459" checksum="B13887FF9A029F11" modified="2022-05-25" version="1" fragment="single">HYFCQPQQQQQQQQHHNHHEPQRQYPNSPTPSVQTTISIDSEDSKYILYYHEKQSSPIPKPFEVHCEVNERRETVYSKLYHLIFPFTPQFEIKAVKLNLFGGYHNILHVIFKDNFSANDYYLLSKDPLIQAKVILDINKQHCQEKSLAYTITLPEDWVISDIKHKTIPMKFSAVEVKYSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNM5</accession>
    <name>A0A8J4PNM5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007942</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000451">
        <property type="protein sequence ID" value="KAF2070743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNM5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035171300" evidence="1">
        <location>
            <begin position="23"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="154" mass="17589" checksum="E815F7B68B8D2F11" modified="2022-05-25" version="1">MTKIELLVYLILFQILIQLINSESVLYCQYCRTNDGGDNQTDSEYQGCNGQAWCNKYQVNECSRFLSPCSEEIGSALFFRIRYSDDIHSFVSSSYNSEQDCINDYSPRSYHTLKDGECEDNYGINVRLYSLSSKTVIPTTTLLLTIVLPIILSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNW2</accession>
    <name>A0A8J4PNW2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007249</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000376">
        <property type="protein sequence ID" value="KAF2071425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNW2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="345"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="351"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="457" mass="52013" checksum="AE2CA6C821997196" modified="2022-05-25" version="1" fragment="single">MALFDQWEIPSIDDGWNYDDDEEMVLNICFDGSGVVKKDSLSEIPEGTVFLSIKIGDAEEKKGTKDRKKTKGSHRDHDESRYVEFDRDYIPQSVTFLGIHHSDSDIRPKVVYRGDWIPSSVTKLAIPSLPFPISPLPEQITYLSLFEQEEPLEIDMVPPTIQHLALMNSNLSHDLPNTIKELVLTTIGPCHDIYIKPSFTSPIQLMYIYSKHSRDKRVVPSNNRLNIQSITFTKRLPRLDAITLPSTLTSLILQPDTFNHSLAALDPLPPHLKYLSLPDTYGQPIYQNQHLPITLTHLELSIAFVGKGPIDFSNDWLCRKGYLPPSITHLRLKFVVLLYEDEESNEEDEKATEKQEDASEVATKEQEVDDDNQIDAKKIKLNNGAHSTTLKEEEVQEFPIPSALIILLPSSIQSICLEPDDDLHRSRIKVIDRDDYYSTTPTPPKIREGLRELVLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNX9</accession>
    <name>A0A8J4PNX9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008227</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000489">
        <property type="protein sequence ID" value="KAF2070462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNX9"/>
    <dbReference type="OrthoDB" id="417426at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="28422" checksum="BE5978EB9ACC36A4" modified="2022-05-25" version="1" fragment="single">SFNQALPKGCLPNSITELEFQRFNQFVFVPPHSLKSLSLSKEFNQELNSSMLPPSLERLSLDTDKEIQIGAIPHGLKYLDLGSNYKHEIKQGVIPNTVEELIITIYDNNLNTTSIPNSVLKLQLKCNVNVKQGIIPSSVTKLCLFDCLANISDIIPNSVEDLEINNGRYKNESFDCNWIPCSVTQLSIKTYILVNLHELPVNTKRLTFESEYYFGQIPSTIEYLQVLNYSRHIRIYNENYEEKEDDKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP16</accession>
    <name>A0A8J4PP16_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Geranylgeranyl pyrophosphate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_007156</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000365">
        <property type="protein sequence ID" value="KAF2071530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP16"/>
    <dbReference type="OrthoDB" id="6921389at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF44">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2071530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="306" mass="35635" checksum="955736D6117F6FFD" modified="2022-05-25" version="1">MQFSYPLTENDYSLLEPYRYICESSGKGFRNILIKAFDYWLNVGENKVKEVSEVVQGLHMASLLINIDDFYSIDDIEDNSKLRRGNPVAHTIYGVPQTINSANFVYFLMIDQCNRLGSPVATSIFIEEVIRLHRGQGYDIMWRDNNRCPTEEEYMRMVSEKTGGLFRLGLRLLQTFSENKTDYSQFIEDLGLYYQIRDDYINLISVDYHSNKSFCEDISEGKFSFPIIHAINSNLNDKRLLMILKQKTEDHQIKTHALDYIQKVGSIEYTRNILKKYEEKLYSQIKQLGGNPILEEMVTKLSNSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP75</accession>
    <name>A0A8J4PP75_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008496</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000528">
        <property type="protein sequence ID" value="KAF2070191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP75"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="113" mass="11879" checksum="CBDE5B964F356BFB" modified="2022-05-25" version="1" fragment="single">FNNVEKVCFATPSNSSIQTCLTFNDGHSECIDNNGASKSCVNYNGNTEACFVPNFHGNQVCVFNNGGSSVCATTDGSVSNTCLTTNGTKDSSSSTLVINSLFIVLAFILVSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPS3</accession>
    <name>A0A8J4PPS3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007410</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000394">
        <property type="protein sequence ID" value="KAF2071273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPS3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035247945" description="EGF-like domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="621"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="640" mass="71262" checksum="B659F60043C1B1BA" modified="2022-05-25" version="1">MIKYLVIFLILVALIKVDASRFSFVKEITIKDNSFPILSCIYKEPNQYYSVDKSKLYYYDLSNAFSNTTTLNEIWSISNTSIPSGGSTQFKLSKYNGTDFIGYLTTQESVIQIGPNLNNFEIKYFETTSPLTSIDSVYSTGGQNNWAYIGNPPSLNLISNGRQYQIKLITPPSGLACNFDQAGCYLSFNTGMSLYLLGQSWGSFVSSSTGQVTPRIGGVVNSRQNVAYYCADSLDDGPVLEAWGSFAQRSTIKLYTFGVWGTCKLMSFDEKQGQIFILTTNSLIGLDTKFRNAQIQSIDASGIISMEAVTQNDNDDQNNYLLMTKISATDSTAKLIITSYESPCLENCNGNGYCQYGKCICKSGNSKCGEEMEILSLFSSVISNDTISVVLSGYFHSPINNASFFVYLDTKNTIISDIISYDSSKIVFLIKKYIPNTSTIGVEINGLTVSISSKLLNPSLEIYNIYQLDQYIHINASHPFIEWYQYSVVLDNDQVEFNLNSTLMIIEPNVRTYSSSYVVSLTTNGIQKKYTVDMKPVILDQKPTIFQTTGDPLELTGLYFGLEPILLLDDKDLSIKYYDLNGQFISTKLPSGVYSNMTIYNGNLKTIQPIVFSPSANSSSSIFNCSFVFFISTFLLVLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPT2</accession>
    <name>A0A8J4PPT2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">isopentenyl-diphosphate Delta-isomerase</fullName>
            <ecNumber evidence="4">5.3.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_008865</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the IPP isomerase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.2" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000590">
        <property type="protein sequence ID" value="KAF2069814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPT2"/>
    <dbReference type="OrthoDB" id="510307at2759"/>
    <dbReference type="UniPathway" id="UPA00059">
        <property type="reaction ID" value="UER00104"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004452">
        <property type="term" value="F:isopentenyl-diphosphate delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050992">
        <property type="term" value="P:dimethylallyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009240">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006694">
        <property type="term" value="P:steroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02885">
        <property type="entry name" value="NUDIX_IPP_Isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000012">
        <property type="entry name" value="Isopentenyl-diphosphate Delta-isomerase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011876">
        <property type="entry name" value="IsopentenylPP_isomerase_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02150">
        <property type="entry name" value="IPP_isom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885:SF0">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0752">Steroid biosynthesis</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="12">
        <location>
            <begin position="111"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2069814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="292" mass="34009" checksum="315240D5822CA72D" modified="2022-05-25" version="1">MLKKLFFHSSVPTTATLLNKQYIANSRSLLGNRGVLQKYNSRNYSTITTTTTNTTITSNNNTRMDTPFQGHDQVQIELMKEECIVVDNDDKPIRAGSKKECHLMTNINQGLLHRAFSIFLFNSEGKLLLQQRALEKITFPGYWTNTVCSHPLWMESELIEKDAYGVRHAAQRKLLHELGVPHEQAPLDDFTFMTKIHYLSESKEDPQWGEHEIDHILVIQKDVTVNPEPNEVMDYKYVDKEELTQMFKDADENKIKVTPWFKLIALNHLDPWWNNLKNLKPLVEPTDKIHRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ30</accession>
    <name>A0A8J4PQ30_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleoprotein TPR/MLP1-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_006770</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000327">
        <property type="protein sequence ID" value="KAF2071913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="20472at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012929">
        <property type="entry name" value="Nucleoprot-TPR/MLP1-2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18898:SF2">
        <property type="entry name" value="NUCLEOPROTEIN TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18898">
        <property type="entry name" value="NUCLEOPROTEIN TPR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07926">
        <property type="entry name" value="TPR_MLP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleoprotein TPR/MLP1-2" evidence="3">
        <location>
            <begin position="1112"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="674"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="913"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="990"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1658"/>
            <end position="1714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1744"/>
            <end position="2004"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="36"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="131"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="444"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="575"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="714"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="809"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1018"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1169"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1285"/>
            <end position="1518"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1557"/>
            <end position="1584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="930"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="990"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1658"/>
            <end position="1668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1669"/>
            <end position="1714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1751"/>
            <end position="1773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1783"/>
            <end position="1802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1808"/>
            <end position="1823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1856"/>
            <end position="1867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1870"/>
            <end position="1886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1889"/>
            <end position="1911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1912"/>
            <end position="1932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1969"/>
            <end position="1985"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1986"/>
            <end position="1998"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2071913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="2004" mass="227646" checksum="C652FCEFD2F990C1" modified="2022-05-25" version="1">MTTVEGVTPQTNSWNCSFITRDEYNQYVASNPIAIVLKAESKIKQLNQSLESIQAKSSVEITNSQQLFQQLEKDFIDNKKELEQLSQQNGKLKNEIQVCTQKNDVLMLDIVKYKELSIEKESKSNTFSKLYDELQQEKSSLYSIIERKEKDLKEETEAVTKLTERLNEFIREKADLKTKLLAIENDKNSQDYQISRLQQEISELKKQNQFLDDNYKSQTNNFFNESKELKSFNLELKSKNETMNEENQRLLQANLTLRDKLKESQAQLTALKESCTKLTEEKLNMTNQFENELETQKRLVGLYKENADESSSKVVALQNQIDQLKECDRNLRKEHEEAYKKMEEAYDDLQEDYQKIIQEYEDYKASNANAASVSSVNGDLSQMDIPILSDQVKDRIIHTTNLGSRDDIRRELLDMVQRYEDLTKISLNEKKEKRFVQDHLNFILREVEKKAPLLQERNKEYQRLLKSQEKLLNSYQELQSQKDTMSLRSTTLVAENNKLRQETQDLQKQVRCLLQEALETKNNLNSTGLSRSTISNSNNFNASVSRYQQQQQQQQGLNTEDDQIIPDSLITFTNIEDLQLRNQDLLRQTRILSQQIQSQSENNKKLEIALNELEHLKANRQRQSEMIDSLMKQMDYMKNNNNAIRNRNSIGGNINNNNNTISTSSPLTLDDLNNSFNSGNNHSSNNNNNSIMYSNNNNNLNTSGPNNFNNDQVLESLQKEYEQLKANYSQLQKDYEQNIKDRVANDKEMLAKIDTLKDQCTKLRISESQLEGEKTLLKERLVITSDSLKSQTAEATDYRQKTQEYVQIIVKHQQQLELLNTSISRAEELRQQAELRLSHMKGENELLKSSEQRLLEMNKSLGYEKLSLETLLDKVNQLNSSKESSELELRRRIENELTSQEKIRISLKKELENEKQQHKETSQTLNHQLNDLRERNEKKEKEMNDLKESLIKLRVSEQTLLQKVSSLESQLNSIESRYNSIIEKSNSSSVVNVPSNVTNDSSSNNNQQQSQQQDSLGLTLAKSEIESLKELLSQEKQSMLHYKSIAISNEADLGKIKEEMTVVKVQLEEQLKDAQLELSKAQETINEQQVKLEKLESFSSTYQKDINTITAEKDSLEKEKELLQEKIKQLTVDQEAAIQESRVQHELCKKATENYERELLLHAEDIKILPTLRSELQEAKNRILSLTTRMESSVHEFENARSSWSQQENLFKSQISELEERLKDLKHHNSLLQAQIENIHSQSSQIDRITSMMVDSNNASSTATSGAEEEINNLRQLVQVQSREQKIIEVKNESLQQENMRLKQTLQHNQKIIDSLNQKLVEEQENMKNLTVSTKKHQEVLQQLDQMNLFKESNMMLKNETKRLAQQIEELGTKLAQAQDKMTPLVQENSRLVGENQILRNEIEAQKVEISNWSTKVQKLLNKYQSIDPEIHQKLLESNETLTHDLQTATTALDDLKRQYDEVKKHAKHWKDESKKGADIKKEKAELELTKNALEAQLTALKDQNTQIKTQLDKAQETITANQTTLETYEKMNRENQRKLLLVNKQQKNTATSAEEINTLKTEITTLKTEIETLKKTIEDKDKENFMLKTKLRVFESMKKPAPAIPATPATPVAPVAFTPSTPPAATPVATVAPGTPIPPQITEPGLKKRVLRKPAPSSLQSLLDPSKTTITTAPPATTTVIPTAPAVPTTTTTTPTVSTPTQITSPTVLSTTPSPSIFSSPFKPAATAAVPALSATSQVFSPQTISTSAPQPVITTQPTTTSTKVTPINTIPTSPPPEEEQQQPSTTSTTASTIETAPAPAAVSSIEELDEEFGNADEESIDSMESSQHATKKQKIDNDSSVVTLSDDEVDIEDTNSNTDNGNNIANTGEEDTNGENNEEGEIDDGAMNTTADENQNSPNTVLNNNEQSNTTTPSTTTTTTTTSTPTDTPTQPKRRLKLKSTVDTQPPDFVVMPAVSMAKSLASRMKPASASTPTTIDSTPTTPQQEESSDASTTTKTESENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQI0</accession>
    <name>A0A8J4PQI0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rab GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_007652</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000420">
        <property type="protein sequence ID" value="KAF2071031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQI0"/>
    <dbReference type="OrthoDB" id="63533at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001447">
        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2071031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="180" mass="21270" checksum="F2EEC9B14DD6A2A3" modified="2022-05-25" version="1">MTRYVENTFSENYLETISVDFRIKCFEGLHSTKRLCLQIWNNRSDERYRYFPVDYHKNSDVLFIFFDITNRESFDQLNSSLQQIHHTQEHYQEIYIVGNKIDLESKRMVGREEAKEFAQENGSIYIEISAKDGTNIKETFDEIAKSFFLRKINDEIPLTPIKGPPPLVVSENPRNTCITQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR06</accession>
    <name>A0A8J4PR06_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_006421</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000295">
        <property type="protein sequence ID" value="KAF2072267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR06"/>
    <dbReference type="OrthoDB" id="2017201at2759"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008764">
        <property type="term" value="F:UDP-N-acetylmuramoylalanine-D-glutamate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="entry name" value="Mur ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00639">
        <property type="entry name" value="MurD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005762">
        <property type="entry name" value="MurD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01087">
        <property type="entry name" value="murD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43692">
        <property type="entry name" value="UDP-N-ACETYLMURAMOYLALANINE--D-GLUTAMATE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43692:SF1">
        <property type="entry name" value="UDP-N-ACETYLMURAMOYLALANINE--D-GLUTAMATE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21799">
        <property type="entry name" value="MurD-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51984">
        <property type="entry name" value="MurCD N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="MurD-like peptide ligases, peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur ligase central" evidence="8">
        <location>
            <begin position="119"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Mur ligase C-terminal" evidence="7">
        <location>
            <begin position="312"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2072267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="449" mass="49036" checksum="BB38E3E53E0CD501" modified="2022-05-25" version="1">MEKHIDECNILKNKKVVIVGLGITGVSCIDYFLSRGISPKVIDTREDPPDLINLRGKVETHVGGFNDDWIMESDLIIVSPSIGKSHPSLKGALEKGIEILGDIELFCREINGKAPIVAITGTNGKSTVTTLVGLMAKESGLNVAVGGNIGVPALSLLDRPYDLYVLELSSYQLETIYTLNPAASTILNVTEDHMYRYPKGMEEYREVKLRIYEKSKACIINLDDPSTFPINGIDSGCIGFGIDNGQYHIAQCDKGETWLNVRGENVLNVRNMKISNQHNHLNALAAIALSEAVGIPLSSCLKVLQTFSGLPHRFHLAFENKGVRWINDSKATNVGSTLASLSGINMDKGTLWLLLGGEGKGMDFTPLKPCLMNRNVRIYCFGKDGPQLATLLPSVTTETANMEEAMRLLAKQTQPNDIVLLAPACASIDQFKNFEERGNIFTSLAKELG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRV8</accession>
    <name>A0A8J4PRV8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_006101</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000267">
        <property type="protein sequence ID" value="KAF2072578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRV8"/>
    <dbReference type="OrthoDB" id="19695at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="256"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="469"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="652"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="18"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="652"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="674"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="685"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2072578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="717" mass="83443" checksum="EA8758189A778848" modified="2022-05-25" version="1">MGDRYNNKNNKQFSNKGYNNSNDRYSNNNQYNDINKGYNNNNSYKNNNNKNNDRYNNNTQYNDINKGYNNKNNDNKYNNNNSKYNQNNNNKFNNNKDYNNNKNNHNKKKEDEEDEDNIKVYDIIEPEQDDDEDNTKLDEEEIDHIFDVVSDKKIEFTIDLEAVDYSDLSYYPSFKKKFLIQETEYELTSSKDFTILNDNTFDDEDNLQEQQQIVYLPDSKIPVASNQKSNCLPKEVKLFFDKTEFTEPTLFQKYAWPAILTGHDVIGTSLPGSGKTIGYLAPMISHVLDRMANSEKDIQDYEIESNIFSLILVPTRELGVQVYNNFKIITKFFGIRALPIYGGVAKQPQIQSLKKINPHVLISTPGRLIEMVYNQRVDLRSVSMLVLDEADKMLSKGLLPQLKQIKTQIRPDSQNILFSATFSESMRDIAKDWIKEPHINLKIGSMELPNLKHITQEVQFHAHHKKPRALVKVLESAELEKKKTIVFFNKIKELKRISIMCLKSNIKHQTIFGNIDQEKRETLINNFKSSRNLLLFSTDVIGRGIHVDDIQVIINYDFPKSLEQYCHRVGRAGRTKKAENAKAISFFTNTDSFLAKAFVNFLTEQGQKPSVPFLKQCNERIGTNFELVLSKRKEKELKKKRAAELEKLLQDVDESDSDSDLDDKYKKKYSGSESESESEEEKEEKEEKETEKKENVGEKRSFVNFDDLFKKNKKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSC7</accession>
    <name>A0A8J4PSC7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TRF2/HOY1 PH-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006594</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000311">
        <property type="protein sequence ID" value="KAF2072095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSC7"/>
    <dbReference type="OrthoDB" id="1516808at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057939">
        <property type="entry name" value="TRF2_HOY1_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33494:SF1">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33494">
        <property type="entry name" value="OS02G0793800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24818">
        <property type="entry name" value="PH_TRF2_HOY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRF2/HOY1 PH-like" evidence="2">
        <location>
            <begin position="313"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="459"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="477"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="538"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="727" mass="81154" checksum="BFE88EF8D9FB8244" modified="2022-05-25" version="1">MTSNINSPTLVPELLKDNCPSPFNSTLFSVPTFNNNTNLLSSSSPALLTNTNNSYSTMFPNISLDGSSASAVGGFGKEYLNPFLFTNSSMGFNLSPTNVPNGPLTPTSGFLNLNATNSLTGTPILSGNSPAGTPKVTPLDISQKNELRHKLISKSGNSAPSSPVQSSQPVSPMLHMQYIPSPQPIGSPSLTNSPMMVPFLSEYDTHPQYRRPSYPSPQELMFPYGSYPGIPYYQYYPQYITPYGFAPPPASASAAAVAAAQHQQLRQHQHQQQQQYSHTGFSIDGSTDSLNKSNSLNSSNTSIDTEVKLKPSNFPAFKISIGSWEYSAGFPGDIIAKFYFTKKQVVWELLKFGVKNKMVASFNDITAIIITNLSDDSASLTVEMSKIPKFYKEVNPQPKKNTMWVSCNDFTTNLSASTFRRHVLTFKKPALMKNIEKLQQSDVRLKKLLENQEYASKDIYLPGENPDSPDYYEKSANSSPLDNNRNNNNNNNNNNNNNNSNNNNSSSNGYNNNSNNNNNCNNNNSNNNTNNENELNKGLSSPLTDSTSVLTESPSFLNSEHLSLAENSDQYSIPKEYNDTFTSFMLTNSLIPQPPLEINNNNEIYNNNDNKLNQQQSEYSLSSSTDQDYNSSSYLNQPSFNNNQQQQVVDYQNYVPSLSNSTPQTTMFYKTNYTQSPDAQQQQQMMNFYIQQQQQQQNVNRNQRYFSNLENNNQQIDDNRCKKAKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSH4</accession>
    <name>A0A8J4PSH4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">nitric oxide dioxygenase</fullName>
            <ecNumber evidence="2">1.14.12.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_006288</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2 nitric oxide + NADH + 2 O2 = 2 nitrate + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19469"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.14.12.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>2 nitric oxide + NADPH + 2 O2 = 2 nitrate + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19465"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.12.17"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the globin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.12.17" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000284">
        <property type="protein sequence ID" value="KAF2072396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSH4"/>
    <dbReference type="OrthoDB" id="275556at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008941">
        <property type="term" value="F:nitric oxide dioxygenase NAD(P)H activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005344">
        <property type="term" value="F:oxygen carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071500">
        <property type="term" value="P:cellular response to nitrosative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046210">
        <property type="term" value="P:nitric oxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06184">
        <property type="entry name" value="flavohem_like_fad_nad_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.490.10:FF:000003">
        <property type="entry name" value="Flavohemoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396">
        <property type="entry name" value="FLAVOHEMOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396:SF3">
        <property type="entry name" value="FLAVOHEMOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00042">
        <property type="entry name" value="Globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0216">Detoxification</keyword>
    <keyword evidence="4 10" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0561">Oxygen transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Globin" evidence="11">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="12">
        <location>
            <begin position="153"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2072396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="415" mass="46794" checksum="708DF46565AB8ED6" modified="2022-05-25" version="1">MDIENIIKFIQESIPLLEEHGGKIATRLYELLFSRNPELLNIFNIANQKGGKQQLALSSTILHIAKHVEKIPEILPAVKKISHKHVALGIKPEHYLLVGESMLTAIHEVLGDDLATPEMMQAWSDVYDLISSAFCDVESEIYEDVEDKSGGWDGLREFVIAKKVEETPDITSFYLKPQDGKLISSYDALNYITTSIEVNDIIHLRHYSISDYPIPSNPRRKNPDYYRITVKKIPGNCSKGSQAGVISNHLHNNTKEGDIINLSAPNGEDFNMHESNEPLILIGAGVGITSCFALMKATLHLQPKRKIIFIQRHKNSEAAPFREELKTIIENNENEKKTLKVITSYSHPLDSDQSDHSGEIDKPWLEGIVQEYSELSNVFLCGPVPFMKHVNSLLKEIGFEKERVIYELFGPLADI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSH6</accession>
    <name>A0A8J4PSH6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DOCK family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_006237</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the DOCK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000279">
        <property type="protein sequence ID" value="KAF2072444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="18896at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08679">
        <property type="entry name" value="C2_DOCK180_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11684">
        <property type="entry name" value="DHR2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027007">
        <property type="entry name" value="C2_DOCK-type_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043161">
        <property type="entry name" value="DOCK_C_lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043162">
        <property type="entry name" value="DOCK_C_lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027357">
        <property type="entry name" value="DOCKER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046769">
        <property type="entry name" value="DOCKER_Lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046770">
        <property type="entry name" value="DOCKER_Lobe_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046773">
        <property type="entry name" value="DOCKER_Lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056372">
        <property type="entry name" value="TPR_DOCK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653:SF5">
        <property type="entry name" value="DOCK FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06920">
        <property type="entry name" value="DHR-2_Lobe_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20422">
        <property type="entry name" value="DHR-2_Lobe_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20421">
        <property type="entry name" value="DHR-2_Lobe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14429">
        <property type="entry name" value="DOCK-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23554">
        <property type="entry name" value="TPR_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51650">
        <property type="entry name" value="C2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51651">
        <property type="entry name" value="DOCKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 DOCK-type" evidence="4">
        <location>
            <begin position="863"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="domain" description="DOCKER" evidence="5">
        <location>
            <begin position="1721"/>
            <end position="2145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1019"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="43"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="85"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="185"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="225"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1021"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1031"/>
            <end position="1044"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2072444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2356" mass="265102" checksum="C675D2FB9E984A0D" modified="2022-05-25" version="1">MNPSSQNNGAPKPAVGKLPTQLWGGAPSGDQPKIVPNFKIAARKPAAAGATGGESATTTASAASTTTTNTNTPTTINKPVVPTSTPTPAPGTTPAPPGVAKLAPRGFLKPAPATGDATTSSSSTTTPTPTNGLKPSPLMKPRVVTDSPAAAVGSTSSATTPTSTPPLTGTVVRKVAPLGRPLAKPPTSNSSTPSSTSPALISPKSSSTPTPLSLEELSVSDDSKNTIVKSASRDNISSTGSSPLVKSVSRENISNSTNSSPLVKSISRDNISSTNSSPLVKSVSRENISSSSTNSSPIIKSESKETIHAITPDTSKTITQPTPNVTTPIIKQQQQQQQQQYEKRESSSVAKESTKNIKAAKKHLERVRVLSNRKGDFLTLNVQRNDILEIVEKGEKDEYQGSNGYKTSAFSISNTKTLFPLDNEPPTNTVKAIAVKDIKGNKNFTIIRGDLLCVLDGTSKKEQDVSVGRVKVGLDGWGELGDFPNTSIRLIGEEEYSLYSDLLDVIIEVSERLMDPDSFAINMPPNFTPKETEKEIERRQLVLRDRVKSIQLIREKILNASTDFSQLNISLKKEIELTRAFLNNEFYIINDSGLIIDTDMQSPLKLLKDHYSINGEKKSMKSDKLILVNSKDTTQADHHHHQTSASSRRQWEYGQIMIDVKLSRCTLPKKNYEFYFSLYNFTQSRYLTEQIMVPYDTTTSTNAPSFKFLFKNIEPADISEDICLVCKVVRKGTFKDIDEGQIKKGVNTDFRRPYGYACIGISVLDQTNERDSTLLFFSSDSDLNYSLLPDYLMKNNEEEIKKHVETIPPKATGPGAQVQQSGLPISLAFYDIPYDAFIQKNSLMGSLPVVEKLEHKVVFSESTHKFYLTLDYGKFTQGKKIEVCIRVRLDSGDFLPNCMSLGCSSSFVSELKSMVCYNTTSTVWNEFIHFSIPEGDFAKCHLLFTVRTCSSSGGNKEKNSIFAFAFLKLGNQDSTVLGNGDHNIVLYKTNSDIINVAHYIDISSSSNLIDNNGGAPTNALSNSGSGASGSSKDKDSSSKSTVRKNESLKVKSLLHSNRYIQHQSIIQLLNWKSHKEELPNLLDKFKFVEPIQIMRNLTKILDALFTIYDSTSMDDLAAGVGSKKSEPIGLMVYNTIVFIIGLLTDERTARFKQFKTQMDDYINNQFHFGNAHKHLLKCISYHLYDASHEDSAKISSTLKALEYIFQLITKSRLVYNKEKSTANEQDQGWKKELLDFLSMLNRLMSNVHPLLIVVKTMALRNYAVVMKGLNNFFTKQELCSILSEFIESVHYTEKQEHLNSYKLSVYYKILSTQLILDSETLLPLLPVLIATIDQHMTRGEELKLSTQLLALLLESIDQVSDQQTKLKCFNMTMIVFTKVMNLGESTLTALATEFQLGGASKNNNSTVTDPFVYDTTFLITSLFTMIQNLINLVNNNSTTLFEKLLLDMQPQENHIKMFIRKLFKIFDGFLEYGLFSPKWPTLLLFHQKTTLDAMNILSTYFYKHFKINNTTTNTVDVETWTLFLNIHFSFLISQPLRLNQNKINRLNYIQADLNQIRVRVLNSLLKAFDSLVQLNCQNQFYQTITEGLLYSLLNGNDDVNEFIQDLFYQLLKTEYNNTGGAKDKSSLSSFKKVESKTIEIIDKITIRERVCDEEIFKIFLNKRLIPSIDANASASDPFSKDARYFVSNITQLLSLLFDFRTLPTDRAFEEERTIATLKMMEYFKDRKDTYIKYLYELLNQHLTNNYFTEAGFAFLLHSDLYEWNEVVNVPLFNVLLQGQQQQISFPQETSSERKVRLIKMAIQYLDKGQVWEKCISLIQELKNHFEATYNFKSLSEILFQEAEFYEKILTIERFYCEYFRVGYYGKKFPVTIQGKEFLYRGFELERLSDFSARILAKFPNAELLKSTSEPTAEIQNSDGQYLLITIVTPSSIEEVQKKQKTFIKGTPANSINYLKKNDIKIFVYSKAFEKADAPKVSTNPNNKFGDLWIRNHFLYTDSSFPTIHRRAEVINKSFIELSPIENAINSVTSKNEELDEMVNKYEKNAQLNLNPLAMALNGIIDAAVNGGINLYREVFFDVPESKRPDQSFLSKLSLGIKKQAAIVEKGLVIFSSRCPEDLKGLNEKLESFFTKFKADMKILAANLDGTNRHKDLNTLPKLTSKVVKYFDIIKPSFFTLTETTATFGKKYLAPKISENYVLFSDKLTYDHIITGYDSDVYDFVGPYRYVSSYGKYQSFMIEDKKTKEKILYTSLHHPHKKNKKLARDLTLKHIEEQMKDFDIDHIVVGGDFNSESDDISDHFSALSLKPTFNGDVTTIKGSSIDNILISESIDVKEKKVHKQNSKLSHFPISATLSILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSV4</accession>
    <name>A0A8J4PSV4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carbohydrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005490</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000220">
        <property type="protein sequence ID" value="KAF2073208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSV4"/>
    <dbReference type="OrthoDB" id="17104at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019028">
        <property type="entry name" value="CBM_49"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052879">
        <property type="entry name" value="Dd_Spore_Germination_Stalk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33239:SF9">
        <property type="entry name" value="CARBOHYDRATE BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33239">
        <property type="entry name" value="CELLULOSE-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09478">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01063">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035290202" description="Carbohydrate binding domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate binding" evidence="2">
        <location>
            <begin position="53"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="152" mass="17092" checksum="335F5AE5B6F29C86" modified="2022-05-25" version="1">MKFISALLFVLIAVSFSSAMADHQCGPHNRCPDMHACVCDAGVFRCVFRCDEVIITQTVTRRWSDDNGQMSYSQVEVEIKNVGHRKVKDVTIGTVGMSLKDNNAIWGISVEDCDIHLPSYIDLLPHQSHKFGYINRGNMAAHLFVKNINIHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTD9</accession>
    <name>A0A8J4PTD9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Rab GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004416</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000155">
        <property type="protein sequence ID" value="KAF2074283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTD9"/>
    <dbReference type="OrthoDB" id="63533at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01860">
        <property type="entry name" value="Rab5_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001667">
        <property type="entry name" value="Rab family gtpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="173"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="193"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="22458" checksum="6ED1EE8103C505B5" modified="2022-05-25" version="1">MNNNNKIFQFKLVLLGEAAVGKSSLVLRFVRGHFLDYQESTIGAAFLAQTVCLNDTTVKFEIWDTAGQERYHTLAPMYYRGAQAAIVVYDIRSEDSFERAIKWVKELQRQGSPNIVIALAGNKLDLAAKRKVETAEAQQYAEENGLLFMETSAKTAQNVNELFVEIAKKLPKTPTTRPGSGRLPITPIDNNAAKKKTCCNGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUR2</accession>
    <name>A0A8J4PUR2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Raptor N-terminal CASPase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_005655</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat RAPTOR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000231">
        <property type="protein sequence ID" value="KAF2073039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10262360at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030307">
        <property type="term" value="P:positive regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001490">
        <property type="entry name" value="Protein raptor homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000357">
        <property type="entry name" value="HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004083">
        <property type="entry name" value="Raptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029347">
        <property type="entry name" value="Raptor_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12848">
        <property type="entry name" value="REGULATORY-ASSOCIATED PROTEIN OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12848:SF16">
        <property type="entry name" value="REGULATORY-ASSOCIATED PROTEIN OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02985">
        <property type="entry name" value="HEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14538">
        <property type="entry name" value="Raptor_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01547">
        <property type="entry name" value="YEAST176DUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM01302">
        <property type="entry name" value="Raptor_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Raptor N-terminal CASPase-like" evidence="5">
        <location>
            <begin position="69"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="711"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="844"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="875"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="719"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="897"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="907"/>
            <end position="924"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2073039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1367" mass="151604" checksum="357935F1D16DAE25" modified="2022-05-25" version="1">MDNRLSISNNNNNNNNHINNSNNNNNNNNNKKKEKYNKTKAYFHEERHKDNATPDVQRTKIAKWRMRERMKTVSVALVLCLNIGIDPPDVIKTFPCARMECWIDPASQPPQKSLDLIGKTLQSQYEKWQSKARYKQSLDPTVEEVKKLCLSLRRNAKDERVLFHYNGHGVPKPTGNGEIWVFNRNFTQYIPLSIYELQTWMGTPSIYVFDCSAAGLIVNWFNQFAEQRDKELESNGNNPNNGGSSHQRDCILLAACGANEILPMNPDFPADMFTSCLTTPIRIALRWFCSHSILTGITLDMLDRIPGNLSSRRTPLGELNWIFTAVTDTIAWNVLPRHLFQKLFRQDLLVASLFRNYLLAERIMRSANCTPISCPRLPPTYQHPMWQAWDLAADLVISQLPTLLANPDAEFKSSPFFSEQLTAFEVWLEFGSEHKDPPAQLPIVLQVLLSQAHRLRALVLLGKFLDLGPWAVNLALCVGIFPYVLKLLQSPAGDLRHILVFIWAKILALDKSCQMDLVKEGGHAYFISVLSSPNIPADQRTMSAFVLSTICNNCRPGKSACLIGNLLPICLSQIHDPDPMVRRWMVLCIAKMWENSEEAKWAAIKESAHEKVCLLLTDTTPEVRAAAVYALGELIGGAEGSEQRTNIELNLGLTLAVITADCSPMVRKELVIALSRIVSSYETHFVSVAQEIASDERMRAIVESKRLEELKRKKGRSAGGSSKESNTPTNTTPEDGVTPSGTPPYDDTSQSSVYACLWKIILNLCSDPFNQVAIRSQSIVKRISCKFSHEELINHNIYTRTITNQPHLNHSFSNSGGANGNGMNSSIGGVGAVMGGNGLNSSTNSTANLNNSSSGNSGFSLNSSTGSTLRNFVNISRITNKDPRSPTKAKLGAQRSPILSVSGNTANNGGLNVNSSNNSNNNLNGLNHSFSSISLNSNNSNSNINGGNTTPKTTPKLSSTLPGDFYISDNMPFEEDELYSDYYEWSCGYFSKPMMQPAEDLESLESIEKKWKYQRNEQIVEQSMESVLNIINQQPKNFSQIAIFSDQPPTSILQFHPFESYLVASDGIDQISIWNYDDSQKVNSFNNGNLPGTKISALKLINDYDPSMILTAADDGVVRLWRGYESNETLKMVSSWRAFPEFATTGLEPISRLVLDWKKDSGLALVSGDTTDCSKIRVWDIERELGIQDILIGSESYVTCLTNEKGGPLFAAGFNDGSVRLYDQRVQSKYSCVSALLEHKSYIVNIAMPSSLGGASVVSGSSTGEIKFWQSGYECSSTTIHNTDIVSSFSVHDYAPLMACGSPNQRIRIINFAGEEVCTIRYHDGFLGQRIGPVSCVAFHPYSILLGASATDSIISIYSGDSKSKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PV39</accession>
    <name>A0A8J4PV39_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ras GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000098">
        <property type="protein sequence ID" value="KAF2075483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PV39"/>
    <dbReference type="OrthoDB" id="24384at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="383"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="383"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="506" mass="59402" checksum="C6B07F316021572C" modified="2022-05-25" version="1">MFIVTLTGDSEIGKNKILDIFQKRNQIIDVNDNIRRYTRYRFYMKLWNQSRVIVNVHRERAEESYYDKGYHSSMYRGNALCIITHDINSQDSFRNVKRWIGDIERFCAVGTPIIVVGFYSDINDPDNIPREKFESVYPLPYFEFQTKDHTEQDQFYFDKFLLENLELYYNYYYKKNVYPMKEEDNTTTTTEPIVPTDARQDSVDELPIAFSKQFTNVQEQNRYESMFFRVFKNKLLFKLIFKHIHWIHQRYNISAYNFYSSPFRKLIVDKNFDFINKRLLVQDSSSSSGGGSDNSSIINNPLFMWDQFSFINVINFLRVNTDFKLFEMVFNRYQSSFDPSKEMYYFVDLEDLTMKMETFPQILESAVIGGNLKIVKYLSSVTSLAPPSPTSSISSTTSTTSTTSTTSATSTPPTSPIKVFQFTEECLEFAIGFGHKDIVKYLIEEKKVRILNESSYRNLISLSDLHKQYKITKYLDSKKVKSLVFNQASMPPPSPTIIQRLFNKVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PV72</accession>
    <name>A0A8J4PV72_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000096">
        <property type="protein sequence ID" value="KAF2075528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PV72"/>
    <dbReference type="OrthoDB" id="444581at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="496" mass="57161" checksum="327AFF88E1E5A9CF" modified="2022-05-25" version="1">MTISTTNTHRFYSLWRNRYIRDCIRNNVCRDTLIYATIEYLNNNHRYLSLFSYNDKVEYNIIIHFKIKNRDDVIGYVNNKHRDLIDRISIANGVEVAIDNDQQQQQASKVDRFDCNLFHQGLRSLSFYNQHIVGSGKLPDSIRELTIESRLRNNINNPLVDMILSELPPKLELLYLPSGLNINVPTCRLPDTLNEFSFRATHENLKPFIVPQNKVFDCTIDIKTDKDIQWLVQHTWVQQAAFWTKYDATRVLPSHLTSIKIDFLDGRLEKGILPSSLTSLYIFDYNEPLEVGVLPPGLLELDLPDYDHPLEQGHLPDSLTELGLGHYNQPLEPFVLPNNLTRLTMDSFNQECFQANTLPRSLSYLQFWSFKGSFEHVGPLDNPVFNGYKPHLIVDSLHESVATLLQNVTTFKISTNQIGSNVYLKGTAIQDLGFVYMSDRYNLYPGFLPSALQKLDIIGLDIKMDNLIPQSCVYLKTDIVDLNTKLIPLSVKIEKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVK0</accession>
    <name>A0A8J4PVK0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005153</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000198">
        <property type="protein sequence ID" value="KAF2073534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVK0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="786" mass="91187" checksum="B3A8FA197D5B5661" modified="2022-05-25" version="1">MDTSNNTATVLIKSILNNVVIRNHIHQYFTKPLGYYLFCNGQLYRNRENMNLSDIIHTKDQPFILDKLCRFRDILENRYSNSRSNDDDGCNQFKTYYQDWLDNDFNLKKKGQVLNTFDNQELVIHVIREISDLFFRKDKEEVASMLYPLIGIKALSEMVLLFVPDIMQKKSGDVISEIHGKRIVHVRNIDPTLSFFCQDIDTYLALRGDVALLQSILFRQTHLPCFDPIDKEMVKLVLSKSNYYNYDLLSAERRSGIFYDQVFGVRIIKRDDPFFYQYSPSGFGSTIMLCVFNEWVPLVIQFLSTNDWVGSGTRIKFIVQEYDAAQQIVSAVKELKLNNKISFLVYSTSQEAIDIVKLLTAAQHDNMYFSPHLAHQIEDLNFFSKEHSHLFDELSKILENPHIKDLIEYGKKLISLGKKSQKRDWLLVGIAMNELEFVKSCQESDSTQQSIADNLLMAYYCHGKNEMYHYLLSLFDAPELEDYLETSQFIIALKKFDLDGVKEIYNEWSRADQIRNLRHHTFIVLLHSIITASTYKDKQRGIQVLEFIKNEAINSDKSRDVFLGINIAWPLTYLVDKELALTALSYCSLGFQNTLLKNMIPGILVANSLDCLKIVIESLTPSELTTLEPQIQPLLSEYLTYAKSPSLKCALYLFERFQHVIHSSILDDLMSSAVRHNNHTLLDILLHRTKIRLIHTASAQQEQELLDRMILRLNLSSHLVKCNYLRANPEFRLTTIPGQTITEKPPNFSISYFSFEFSQTDTLKNANVFQSSYQRYHSIISKFHSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVL5</accession>
    <name>A0A8J4PVL5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000145">
        <property type="protein sequence ID" value="KAF2074470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVL5"/>
    <dbReference type="OrthoDB" id="16236at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="76" mass="8623" checksum="BF579A32BAA44683" modified="2022-05-25" version="1">MAPKVDMAVLPLVIILGITGVCTAGFGVYRLSTDNDMALSRRRQMLFINNNTPKMVDRETTMKFYNEIHNGKFLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWE1</accession>
    <name>A0A8J4PWE1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Alternative oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_004569</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="12">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alternative oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000164">
        <property type="protein sequence ID" value="KAF2074127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWE1"/>
    <dbReference type="OrthoDB" id="16906at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009916">
        <property type="term" value="F:alternative oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010230">
        <property type="term" value="P:alternative respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01053">
        <property type="entry name" value="AOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.140:FF:000004">
        <property type="entry name" value="Mitochondrial alternative oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.140">
        <property type="entry name" value="Alternative oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002680">
        <property type="entry name" value="AOX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038659">
        <property type="entry name" value="AOX_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31803">
        <property type="entry name" value="ALTERNATIVE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31803:SF3">
        <property type="entry name" value="ALTERNATIVE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01786">
        <property type="entry name" value="AOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005229">
        <property type="entry name" value="AOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 12" id="KW-0408">Iron</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="309"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="309"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR005229-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2074127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="38393" checksum="2B729D3A425E1772" modified="2022-05-25" version="1">MLSKSITSRYLFGKSNQSMPLAFTLFAPSHTTSQFKNINSQRLYSSTSNGNNSKHEIEKKRLEISQSNSPVYHQNSTLYSYTPREVLERIDREFVMPPIYVPVTFSDKLAHSSVKFLRKFSNLFFREKYVSYAIILETIAAVPGMVAGMMTHLKVLRNMENNNWIKILLDESENERMHLLSFMELTKPNMLERGLVAVAQGVFWNLFLICYFINPKTCHRFVGYLEEQAVISYTNFLRDLDAGLVKNVKAPPIAIEYWGLPADATLRDLILVIRQDEVDHRHVNHEISDKIKVNSNDPIRIHNHFLESPETTNNSSVAQDKKDKKTNQLHPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX13</accession>
    <name>A0A8J4PX13_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000106">
        <property type="protein sequence ID" value="KAF2075276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX13"/>
    <dbReference type="OrthoDB" id="21353at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="259"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="299"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="316" mass="35693" checksum="087C49F3D599E4C9" modified="2022-05-25" version="1">MTKKQSFKGPVTKWEKRWVTVGHMKLLKWVPLKTKRINQSVVVTRNKDGTTSRQTVAPNMETRLSRSKRAADRDRAKTDMSDEIQIMYLNSLPKTRRTVKKLDLLQKKQLQEAGIKDDDAAPSSADEKATPTRLQSRRAASNRNLKSPGIESNASDVDNDVADADAIEDTIIGEENGKLNGDQDIKDDNTLLNDGGKDDKDDDEIMDEEDDSKLEEEMDEDDKSDVEEEQDDKSDNDKSDNDDEQDDDDDEQDDEDDTNTNNIINPNLNDMEEEDKTTTNDDNTNPSADKIFDDSFSSGLTDGLNNNDNDTLFNNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX44</accession>
    <name>A0A8J4PX44_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003211</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000098">
        <property type="protein sequence ID" value="KAF2075475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX44"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035217688" evidence="1">
        <location>
            <begin position="18"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="305" mass="33542" checksum="A136B65F309E48E0" modified="2022-05-25" version="1">MKYILLLLAFTIGYINAQIINGYGNLSPSNSYVVDMTTYAKFSRAYRPQPQNMLAFGGLDTGASSYSLNAVYSNSANSITWGLVNYMNGGVSDLETANASSAYNTYQLNGISLVPAIDTLFYLGYQKSSAGQNQVDLITFKFGSQLTLPITVSRVAEGAYDYSSGSGYFVVSVTNSNHYQCFWVDVTTNQVVKNITINEFTPASIYKVFVYKQELYIVQPDSSGNYVEVYVVDWADQVANLVSRVLISIQPIEIQVSLTESYLAIFCVARNADTAIVPLISLSDFTVADSLEYLPVSAGNIFFIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXC9</accession>
    <name>A0A8J4PXC9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002052</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000054">
        <property type="protein sequence ID" value="KAF2076623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXC9"/>
    <dbReference type="OrthoDB" id="294251at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="425"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="464"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="742"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="66"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="352"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="464"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="761"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="770" mass="87046" checksum="4A7E80B420714ED7" modified="2022-05-25" version="1">MGQIDSKEQQQQQQNEQQRLRSSSSGMPPPSPNLMQDVSLANIPYQEIMDLLNKPSGYTSHGYDPGSSNTNTNGVGSSNSGNGSGGSNSSSRSLSRISSINDIPSVSSSINSSGAGISFNNNSSSSISNNMNNGDHQLLLQKQQQQQQQQQQQATQIIDCNGFQLPKEELEPEEEILQRLPIIYPLLNTKPEVVIEKILESNPFSLSIFPPSPSISSSSPSSSPLSLSSSSTFSTTLNAFDVNSSLRMLCLYKQHIELSTSNISIEQDGLIKKIGEMDMLCTKANQLMVKYATFTKQTISQLPDVNKMNAQVKDTQQTMNNILDKIQNIIQYLPDQQDELNYLNQSLNKPISSSSSSSSSSSSSSSSKEKPQLQHHDSNSLLKGVSLTNIIHSFMDKKSLWVDEILPKYESFKGTAKVRELARKGIDKDIRGLVWQSCIGNSLQITPASYSKMVEKIEKINLESNNNLNQGDSDNNSNNNSNNNNNNNNDHYDYLAKTEEKSLIKDSIVNSFSMIEIDLPRTFASFSLVNKETSQFRKQLQRILMSFAQYLPRVGYVQGMSYLGAMFLLHMDEYHSFVCFTNLIGNNRFIESLFHLNMKEIDRYMLCFQEMLLHTLPKLHEHFIDIGFLPHHYLIQWWLTLFCQSLPLNVAIRIWDCFIIEGDLFLYITSLGILFHYRKALEENNLEFCKTFLSNLPSDLDPDLLFKSIDHINISHCLQIWRKFEVENLEMSEELVSFKQSSSSYKNHDDDDQVDNEIDNGVNSNHRSSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXH9</accession>
    <name>A0A8J4PXH9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Choline transporter-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_004808</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Choline transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the CTL (choline transporter-like) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000177">
        <property type="protein sequence ID" value="KAF2073884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXH9"/>
    <dbReference type="OrthoDB" id="420519at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007603">
        <property type="entry name" value="Choline_transptr-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE (SLC FAMILY 44)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385:SF14">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04515">
        <property type="entry name" value="Choline_transpo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="365"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="425"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="457"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="17"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2073884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="531" mass="57814" checksum="EE1F4E3DE56E032A" modified="2022-05-25" version="1">MWAPEEDLKQPLLNGKDSGSSNGFSQQPKVKTERKCNDILFLILFLGVIAGMGVVSGIAYKRGDPSILLPQNEIANHIKNSTESFVISKLENAVAQMRKDKDILIYSVILAVVLGAVWIELLKTFTRFFIYFTLCLGVALVAVLGILFVGLGRKESSESTQIVGFCLIASTILLICVIVYLRKSIDLTCAMFTETCRGVQRSPSVFIVAGIVVLAFVGFLAYWTSSFVYLFSIPGHTVNPFNENSSADINLAGSGSASQNGTTTSVFNTKIRNLMYFMIFGFCWVTAFISAVFQHTVAGTVSHWYFSRNPSGETDVGSSNAFGSLGKALTTSLGSLAFGSLIIGFIEFMQFILQVCKNTNSSNKLVVLVVSCIQCIVGCIEAIVRWINKFGYIYVAMHGTSFCTSTRTCFDLISRNMFSAIVMDFIGSFVLLLGKILGSAASALFTTGLLYGLGRSLNPITIGLSALFAFCIFNLFTHIIGIGTDTIFVCYLEDLETNGPNNLYISPDLHDLLQEKASKSNEGNNNKSSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXM6</accession>
    <name>A0A8J4PXM6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_003476</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MPI phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000109">
        <property type="protein sequence ID" value="KAF2075215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXM6"/>
    <dbReference type="OrthoDB" id="26523at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010971">
        <property type="term" value="P:positive regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110032">
        <property type="term" value="P:positive regulation of G2/MI transition of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000751">
        <property type="entry name" value="MPI_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE DUAL SPECIFICITY PHOSPHATASE CDC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828:SF17">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00716">
        <property type="entry name" value="MPIPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="8">
        <location>
            <begin position="563"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="242"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="383"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="471"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="685"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="251"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="261"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="277"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="313"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="337"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="407"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="685"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="707"/>
            <end position="720"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2075215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="720" mass="79214" checksum="FA51D0E3D305079F" modified="2022-05-25" version="1">MGQNSETTLNLTVTSNTTNTTNTSTSTATKSFLILDKEANKENNINSGNITAFSPMVINHSSKKFGNGVSSPLSISLPNSGNNSNASSPIATNIFPLHNSASTIQQAPPPLFVSSPTASSTSLSSPNFVPINPFLPHAINKSNSDDSLNFLGGGASGAGDNNKPVKKKLRNSQSMFYTTFNHLSIHSQNNSLYQDSPLSRSTSDLSHYSYDIHDSDQFTIKSRPRTLTMESPRLRRLDFGSSKYSSNLSASPPPPPPPSPFLFDNNSNEDSANSASIGSPSTLSLSHPHNTTLYSSPPTSPYKFDGEAPPTPLQNSYQSPHQQRLFSSLGMSNYPSSPEPSPLPPTPIRSGAVDDAEAATNCLFSSAPPTPLCGDLSEQLLAKQHQDDDDSNSPNTSVDITPGSPYKTPSKSITTNTKSPINSSQSHSFVYPLKFKPIVQSPLSHSSDSITVKKRSRTFIDSRGDSHLHCSNNEGFSLYRPNKQSPDISSRPLPTISSNTIGEDNESLLSSSSTTITTTTTTDNINNHLLDGTEYKLMLNTCTGRPGFNNIAPEVLHQLITDNAQNIVVIDCRYSYEYNGGHVKNAINIPPLVSSENLLKMFFDTSLTSDKIIIFHCEFSSKRAPDCYNIFRDMDRRFNNYPTIQYPQIYLLSGGYKRFWETFPTSCDGGYTTMNDSRYTNEFKNEKKREEDLKKEEQRRKNKRSQSFHNFSAYTRSFNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXX0</accession>
    <name>A0A8J4PXX0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002812</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000082">
        <property type="protein sequence ID" value="KAF2075876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXX0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035220467" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="231" mass="26614" checksum="F7C9748B36DA264D" modified="2022-05-25" version="1">MNKNILLILFVFIGPCFSSTQLKYYSNSKCDGVAYKTIDLDKCSMYGAIVTLENGIKWYKDKWLEQKCDESNDYNEYTYGNCYHDDTWEDSFFTVSNEPHKEIQGSCYSSISLSVDFKNGFTIMQTKNASCYKEQRDFNGNYYQSLICTPQKTTVLSCKDSICASCDVSNEIEHTQNDYEYSESYYSRNKYVEADSDTQNKSQKQSSDATALSGPCQLITIVLLLFYFCLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYC7</accession>
    <name>A0A8J4PYC7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Lysosomal integral membrane protein II</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_001361</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD36 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000031">
        <property type="protein sequence ID" value="KAF2077358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYC7"/>
    <dbReference type="OrthoDB" id="195015at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045335">
        <property type="term" value="C:phagocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005044">
        <property type="term" value="F:scavenger receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006911">
        <property type="term" value="P:phagocytosis, engulfment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002159">
        <property type="entry name" value="CD36_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923:SF51">
        <property type="entry name" value="LYSOSOME MEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923">
        <property type="entry name" value="SCAVENGER RECEPTOR CLASS B TYPE-1 SR-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01130">
        <property type="entry name" value="CD36"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01609">
        <property type="entry name" value="CD36FAMILY"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="723"/>
            <end position="748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2077358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="765" mass="83950" checksum="725C0B42D0441152" modified="2022-05-25" version="1">MDKCKIAIFLIGGLVVIGGFALFIVSLLVFPNLIENKINSTIFSKVIVDSPDSFQYSDWAGENSVNNYFLQYYYAWNLTNPNDVILGKKPIYESVGPFNYRYNWEYLNVSFSDNGNLAQYSQKKTYTYDGTTYPGLDPSQVSITNLNPAYLGLMLQFAPLAAQFQLSSEDLLFIVGGSGQMNQFLQFFNSKNFTSVAYFVSNPVLYNKSFQALNTSLVGVVANPVEYIYQQWANATAIPAQGASWNGMLTSLASGQPSGISMKSAQLIFNASNPYSILNDAGLNVWISAYFQDGPSIKSLIDNLGLSASQLDVVMSWWVSILGSTLTTPYFTAQCNIPDLELLGVCQFINLIPLQGKSIATLTYVGQPYTIAGSPVEIASFSSPLTMTPQQAQQNIFSGPLSILGAIGIGEMFNASTSGNYTAWNISVSDGGNIVGYFSQGISPTYTAQSVQALYNNTGGFITTRTVDQWLWNCEDVLLDFLGIVQNCGLQQNGTISKPTTINTGKNNPYLTNTVTIYQTQTNLTAWNGTVAPAGYTESGQFAPNVPVQQNLTIFDENVLRPLNLTYAYNTSVDGIQTCRYYLNNNSFPVDPLFYNSIAGFANLTSVQNLTVFVTLWDMYEVDANYSTARIDGLSPSYENASIPLDLEPITGNALYYNLKLQLNLLVPNNTWFADQIVSPSPYASFPQDIYLPIFKIGQTANPGQSSINLLKSQFKQMKTARIAPVAVGAVVGSLMVVLGILISVLIFHRREQRKQHQGYLAING</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYF4</accession>
    <name>A0A8J4PYF4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_003858</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000127">
        <property type="protein sequence ID" value="KAF2074832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYF4"/>
    <dbReference type="OrthoDB" id="14850at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051052">
        <property type="entry name" value="Diverse_substrate_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942">
        <property type="entry name" value="METHYLTRANSF_11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942:SF4">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="4">
        <location>
            <begin position="53"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2074832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="263" mass="29978" checksum="A3BAD2ECD98B34E5" modified="2022-05-25" version="1">MTSKLESDKSNYKVLGDMFGANSQQYRAFRPQYPEELYEILEQHLDEKRDLALDVGCGSGQAAIKLSTLFKKVIGTDPSESQIKNAIQADNIEYKVLPAEDSGLESNSIDLITVAQAIHWFNLPAFFEESKRLLRKDGVLAFWTYIAMKINNNEEAEKINNKLYDETLIRYFAPQRALVDNGYKDIIPPFENVTRKVFPYIRKMSIGGLLGLYSSWSGYQKFVLTNPDPLPEIKAGLMKAYNTTDLDAPVIEGYWDMYIVICK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYH4</accession>
    <name>A0A8J4PYH4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_001221</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000028">
        <property type="protein sequence ID" value="KAF2077448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYH4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd24028">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000010">
        <property type="entry name" value="heat shock 70 kDa protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000005">
        <property type="entry name" value="Heat shock protein ssb1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2077448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="514" mass="58252" checksum="165441A8EF1A2173" modified="2022-05-25" version="1">MYCIGIDLGSKNTRVAVFRKGRPVVMIPNQYGDRSILSCIIFLADRYLIGEKESLYYENTLFNFKRIIGLKYSQIPLDDTNLWPFKVVNVDGQAHLQVTCDNETKTYSPEELLLIMILKLKENTQKFIGHSIKEASISIPCFFTYPKRKAILKACEIAKLKVRLISEPTAAAMAHDFGPEKKNVLVYDIGAYGANASIVTYEDLIYEVKASFGVYLGGEDFDNCLVGHFVQKLKTKYNKDISQDKRAMFLLKDACENVKIALSYSQQSQIDIKSLFKGTDFTISITRKTFEKCCAHLFQKVTDPIDLVLKDAQMRRTSIDEIILIGGSSYIPKVRQIVKDFFGGKQLKNSLDPDCAVACGAAMYSSSYQYRDDEDILLMNATLFSYGIETEGGVMEPIVHRNTGTPCKIHKTFLPIKEDQKSVLIKIYEGNMMLTRDNILLGVFEFHLPIPKENIPQKIRVAFDFSIITETIKVICLHEATGKEEIFNLTHDKVCSKEETIASQNQELSLRLEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYU1</accession>
    <name>A0A8J4PYU1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001960</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000051">
        <property type="protein sequence ID" value="KAF2076703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYU1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="116" mass="13808" checksum="D509316FC732D921" modified="2022-05-25" version="1" fragment="single">MISDTPTSSNGRNNNDILFYLIWRDHYVNRIIRNKICAGLVININLDYLNKNHQHLSILSTKENCIYIKLSIKDISEFYQYTNHPYRQVVNEISFSKRVDVSPILRLEQQQEKNQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYV6</accession>
    <name>A0A8J4PYV6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002510</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000069">
        <property type="protein sequence ID" value="KAF2076210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYV6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="85" mass="9984" checksum="F98333247B9900F8" modified="2022-05-25" version="1" fragment="single">MVFSVWFSYEDDEVELEDLSNDTTVSAIRKMLHEGKIFRFPYGTSQTDLQIGKLIDQKFEKFKARNTLGDIQFKLGETLYVRVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYX3</accession>
    <name>A0A8J4PYX3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioester reductase (TE) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001937</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000050">
        <property type="protein sequence ID" value="KAF2076748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYX3"/>
    <dbReference type="OrthoDB" id="20372at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44845:SF6">
        <property type="entry name" value="BETA-ALANINE-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44845">
        <property type="entry name" value="CARRIER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioester reductase (TE)" evidence="3">
        <location>
            <begin position="49"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2076748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="404" mass="46795" checksum="133199C70B7B7F9D" modified="2022-05-25" version="1">MTSFSLLQKKTIEYWYNETLLEDNFPTPNNKFLSSQFKNILIIGSNTLLNFYLLKQLLKRDDCESIYILNKFTIDEQQEKQNIFQAIESLTGAEISKDDREKLKLLNSDLKNGQKMFGLSKHIYENLSSTIDIIINSGSIVDFSLSYELSKIENVNGIKQVLKFSNSNPNYLMKIVHLSSISLFFNNEKVLEEKDLPELGLLNGKYGYYQSKTISEYILKEASSRGYKILLIRVPTLISIEKGVVDEFDLFISLLKKSLSNGIFPDMAHFENYNVVQVEWFSRVLFKLITDNSLVIQENLNIFNPSHFTQIRPIVHTISKEFNIPTINMDDWISHIGLSKDISIKNKAAIVNFFCKQKKFISPMSIKTWNYLVGINEHSNINIDYNSFLKFLNIDLNYKKNSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ00</accession>
    <name>A0A8J4PZ00_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000914</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000019">
        <property type="protein sequence ID" value="KAF2077793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ00"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58113">
        <property type="entry name" value="Apolipoprotein A-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="22"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="188"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="278"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="294" mass="34432" checksum="E29C4889A7750EBD" modified="2022-05-25" version="1">MESSRDLWKLFFKLSQDLNNASVKEETMVSELTNQVESLKQKLEEKETQNIRALHEKDARLTEAIANHAEALKEKELQFNQALKEKEAKYTQALKDQDAKYTQALKDQDAKYTQALKDQDAKYTQALKDQEAKYTQALKDQDAKYTQAHKEEVAQYTQTLIDKETIIKAKEIQIDFAQQKEIKLLFELKESENIVEHLKATIAEKNQNNVDLEKRIKHMENNNLMLFNDVKDLIKTHREEKEKLLKDTSDKEEMLKFISKEFPVPTKEKRNAFREFLSEEENEDEGQDGATVNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ47</accession>
    <name>A0A8J4PZ47_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 16">Phosphatidate cytidylyltransferase</fullName>
            <ecNumber evidence="6 16">2.7.7.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">CYY_001820</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>a 1,2-diacyl-sn-glycero-3-phosphate + CTP + H(+) = a CDP-1,2-diacyl-sn-glycerol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:16229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.7.7.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 16">Belongs to the CDS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.41" evidence="6 16"/>
    <dbReference type="EMBL" id="AJWJ01000046">
        <property type="protein sequence ID" value="KAF2076853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ47"/>
    <dbReference type="OrthoDB" id="10260889at2759"/>
    <dbReference type="UniPathway" id="UPA00557">
        <property type="reaction ID" value="UER00614"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004605">
        <property type="term" value="F:phosphatidate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016024">
        <property type="term" value="P:CDP-diacylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016720">
        <property type="entry name" value="PC_Trfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13773">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13773:SF8">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE, PHOTORECEPTOR-SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01148">
        <property type="entry name" value="CTP_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018269">
        <property type="entry name" value="PC_trans_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 16" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12 16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 16" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14 16" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="15 16" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="223"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="25"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR018269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF2076853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="450" mass="51809" checksum="FC71D1B4E935135B" modified="2022-05-25" version="1">MMDIKNRKATTGGNNKTTATQELKNQAKADSRDFDSSKDDESSSEDESNAKQVANNADNAKPNDPPGAKWKKLLVRSIMGAMMIGFFTFVLNTDHFIVSLFVIALQLLVFKEMIGLRYIEAKEKKIPSFRTLNWFFLLSAFFYFYAKPILVKLSEYHPDLFQHFVRYHLWHSFSLYCIGFVTFIFTLRKGLYRYQFSQFTWTLMTLMMVVVQSNFLISNIYQGLIWFILPVSIIVCNDIFAYLNGFFLGKKFINKPLMKISPNKTWEGFIGATFWTLLFAYYFAGFLIQFDWVICPKGNAGFMEALHCTPDPVFLPKEFIIPHEISSILSKYLGLTISSFTFIPIQYHALVLALFGSLIAPFGGFFASGIKRAYKIKDFDTIFPGHGGVTDRTDCQFIMGLFIHVYFNTFIKTLEMDPTQIWAGLMMLPMDEKIQIFEKLKSTIDLSLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZB5</accession>
    <name>A0A8J4PZB5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001734</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000043">
        <property type="protein sequence ID" value="KAF2076958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZB5"/>
    <dbReference type="OrthoDB" id="20232at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035158090" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="267" mass="30387" checksum="322828AE656B5C40" modified="2022-05-25" version="1">MSIYFRICCTIIWLSCCLFSCSESILTHRNFVGFQITSSSISHKFLTFPYLFFDFSSPYPFANSFCLPTKKYDPKKELVFKSGVKSEHSFECSINDPKAVQDMFNEDILIQVGLNPESAVACIMHSKLKNDTVNGQQHVIGFKLIVTASALGFSQTYYSVDICQVDVITVNRELEKNDIFQQIIVLDGGPVFISGSIFIGMGGFILIFSCLILFILSKKWGVDPYSELEDDEEQIGHCESNHFDISYMTEEERKLFYMKKVNDQLMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZE5</accession>
    <name>A0A8J4PZE5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002277</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000061">
        <property type="protein sequence ID" value="KAF2076420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZE5"/>
    <dbReference type="OrthoDB" id="79687at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051177">
        <property type="entry name" value="CIK-Related_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984:SF6">
        <property type="entry name" value="SCY1-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984">
        <property type="entry name" value="SCY1-RELATED S/T PROTEIN KINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="25"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="653"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="729"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="834"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="899"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="986"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="656"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="685"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="729"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="748"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="834"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="986"/>
            <end position="1010"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1016" mass="113681" checksum="8E3ABAF194101DCB" modified="2022-05-25" version="1">MDAVTSKVRGFLGSANNLINPIKDHDLKEQVSSTRFWKIHEGLKKTSNEEVSLFVLDKKLNEKLPKSTQEQINSFVKKEGQALQRLRHPSILNVVSVLEETKTHIYFATEPILSTLEDLMSYYRSRKRASADSSAPSEQSFKLKDFTFEELEVKTGIFQILEGLHFLNHTAKLMHRNISPESIFITKSLKWKLGGLGFTCSMETKEPPIQNLSLADLREFIYQNQDQSCILPNVEYLAPEFILNKRFENNSDLFSVGRLIYELTLNINLEHPSLDHSQISKLGTLAYYNNILDHIRRVTANNTHRLDSAKVCSILLGDTQMRGDLENFIRSSYFQDILTKTLVYIHNIVQKEDESKIQFYRGLLRILSQFSIRLQTNYILPILLQEVANERVSYVVFPNIVAICKENLFKKESFVSQVMPVLQPILTNPNIKPDILACLLENLTLFLTKCNLDTIKRTVVPVVLGTLCGPTQEIIFQCLNTCQPIAKFFDCEIITVGVIPRLTNLCVTGFPIHIRTKAIQWFTILIPSIEKKVIIDTVIPHLEKILAVDNSPPILEALVQTYEAISKKLGGEMLALKVLPALIPLSADKHLDLEQYKTIMRVIKEILQTYESERLNELLNLARYTKPEISEGDKFANVLGNISLPSALSNLGIGGTSTPTTSPPATVQPSLQPITITLPPPPTSPTNNLNNSSNNLFKTSPSNNNLNNSSGSGNKSFLDSPDFNSFGSTNTATSNVTSPVKNTQSNDPFSNFSSIGNNNINSSNNSNLSNAPPLSLNNNNSSSNFNNSFSSLNSSLNSSFNTPLSNNNSFNSNNNNKGGLLDGLSTITPISNNNNNNNFSNNNINSQISSNNMNKSNNNSFFNSPPILEPNKSTSNSNSRYDINLSSYVDNNNSFSTTSNMGSNMNMNSFDTNSYSSQPLQPQSSSSSLYNNNTFTMNSNSSMNSSSNNNNFNFQMNNNNNNNMMMMNNNNNPMNFNNTNNNNMNFFNQNNTNNNNNTNNNTNNNTNNNNKNDLFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0D0</accession>
    <name>A0A8J4Q0D0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001129</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000025">
        <property type="protein sequence ID" value="KAF2077588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0D0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="132" mass="15557" checksum="C6C115A1F6B18B22" modified="2022-05-25" version="1">MFATESVKIVGDISRFKNNADFKSYGLIKNNQVMYEKDFHFNNEDEINQIRNELKRPAVNQTFTINGEKYIIQAQDLLLNFMWAKLANGEKGTLYIRSSNDYKSIFIAHDPHEFYVGTYDKYIEFTNDILTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0J7</accession>
    <name>A0A8J4Q0J7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">SAM-dependent MTase RsmB/NOP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_002217</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000059">
        <property type="protein sequence ID" value="KAF2076477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0J7"/>
    <dbReference type="OrthoDB" id="427002at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009383">
        <property type="term" value="F:rRNA (cytosine-C5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1170:FF:000001">
        <property type="entry name" value="Ribosomal RNA methyltransferase Nop2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1170">
        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011023">
        <property type="entry name" value="Nop2p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023273">
        <property type="entry name" value="RCMT_NOP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054728">
        <property type="entry name" value="RsmB-like_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00446">
        <property type="entry name" value="nop2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF30">
        <property type="entry name" value="28S RRNA (CYTOSINE(4447)-C(5))-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22458">
        <property type="entry name" value="RsmF-B_ferredox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02012">
        <property type="entry name" value="RCMTNOP2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="11">
        <location>
            <begin position="266"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="16"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="556"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="16"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="92"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="115"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="130"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="177"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="556"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="576"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="601"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="615"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="482"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="381"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2076477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="634" mass="70859" checksum="00980E7DA74FB040" modified="2022-05-25" version="1">MAVDKKILAAAAAKKQVAAKAAPAPQKKVVGAKPVATKVSVPPPKKQTTPSKPQPKKAVKQPEPESEEEDEEEEEEDFSDEEEESTNKSNLFEDDDEEDEDEDDEDFNNRNTKAFSDENQKWLKVKGQEGEDDDEDEEDDEEEDGLEAQSRLIKQKKDKIESENIDEIQKEHKKSIDEEDKLDIEEEKEEGEAQSELMGVDLPAVYQRIKDVIQTLENFAKERKQGTTRQQYMGRLAQDISTYFGYSLWLVDVFLKIFPVGEALEFFEANETHRPLTIRANTLKTRRKDLAEALIARGVHLEPIKWSQVGLTIYDTQVAIGATPEYLAGHYIQQSASSFLPVLALAPQPNERVLDMCASPGGKTTYIAAMMKNTGVLVANDVNKDRIKSLVANIHRLGVKNASVSNLDGREYPTVMGGFDRCLVDAPCVGLGVISKDPQIKLSKSERDVITCTHSQKELLLHAIDSVDADSPTGGIIVYSTCSLTVEENEAVVDYALKKRDVKIVDTTIDFGVQGFTHYRDSHFHPSLILTRRYYPHTHNMDGFYVAKLQKISNKIPNGESTGAKQQVKKRKNPASDSEASNTSSSESESSNKNINKKAKTTTANNTNTNNKVSNQKKGKGKNRYFKSKINQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q115</accession>
    <name>A0A8J4Q115_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Monalysin Pore-forming domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002584</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000072">
        <property type="protein sequence ID" value="KAF2076122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q115"/>
    <dbReference type="OrthoDB" id="2368263at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031157">
        <property type="term" value="P:regulation of aggregate size involved in sorocarp development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040927">
        <property type="entry name" value="PF_Monalysin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038768">
        <property type="entry name" value="SmlA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35884:SF1">
        <property type="entry name" value="MONALYSIN BETA BARREL PORE-FORMING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35884">
        <property type="entry name" value="SMALL AGGREGATE FORMATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18063">
        <property type="entry name" value="BB_PF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Monalysin Pore-forming" evidence="1">
        <location>
            <begin position="51"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="261" mass="28952" checksum="46DF3909706A5944" modified="2022-05-25" version="1">MSTERGPLHPSTLNIPLSLSTADGDIHKADLENAKKIGVPTASSNFDPAKSRTCNSNFEIDEAIKLKSPPDSMVMVNAPWTEMAGEVPFKVKPICAYMNYINSLTVPGTIEKTLEKKNGYSSRFQTSVEIKAGVSAGIFGCNASLEVSTGFTYEETITSETTETWREILESGSYVIYQNCVLYAYWVQQPGGINGAVKVARLMGEYAESGIQWYEREGESYFFVPVFRNDPFTLKYTDALYAPVPYNDIISYVTNSGSNRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q149</accession>
    <name>A0A8J4Q149_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002772</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000080">
        <property type="protein sequence ID" value="KAF2075924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q149"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2075924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="101" mass="11214" checksum="307338EDF2970EB2" modified="2022-05-25" version="1">MSASQNTLVGLFVATSFFFGASYGTQKFNSLRDQARDTLKKDIKKITREEKAKSYEEGYKAAVAAAQPKTQSTQAYENEFEELMGNILGDEFVAEARLASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q198</accession>
    <name>A0A8J4Q198_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002674</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000076">
        <property type="protein sequence ID" value="KAF2076014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q198"/>
    <dbReference type="OrthoDB" id="16189at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="17"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="67"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="107"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="231"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="307"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="414" mass="46782" checksum="2B6B707F85E57D3D" modified="2022-05-25" version="1">MGLSFPIEDKLKWITNLRVQILVLTLVGWGIGMAIDRTEVNKYRMRADDIFTAAGNMGMVRTDGHKAALIILSIIETLLFGLGVGFFIAVGFRVFKIQNKMLRKRMIWCWACISFFMCSWWPHSTSHSLIFLSSADPIDNYIAMELSFHWTIMFCSLTLAYFQYDLISLMYEVAMMRLKMKENREVDPLKKQWYHNFKIHALVIIAIMWGVCMAIFFHFDPLDKTMKTYQIFFYVTFKIVDSVVCAIGFGFAYVAVRTIVPLPSNAGKKVSIISSCCITFLFLIQYPHPMVHTKLPATMTNVVAVDYGIHIPIIIATAVLAFYQFRMLELATDAKSSLSVAAQMRRKTSTAASNTNGISMDRQGETGNTGYSGSFGTSSVEESLSKDPSVNIEEVKAPKSVQVEIEIDIDKDAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1K7</accession>
    <name>A0A8J4Q1K7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001566</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000038">
        <property type="protein sequence ID" value="KAF2077119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1K7"/>
    <dbReference type="OrthoDB" id="10691888at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="724" mass="84488" checksum="0CCDF15973F81932" modified="2022-05-25" version="1">MSSCNNTTKSVSKLYRYWCIKQPLHQRTLSIQRLSPLNTDSNNKSSLFFVNKPYQRNFFASNNNKTQLSPTEQSSLVDFLDSVSSQKDYINIKSVEEQEKEKQQSTIVDSPLPDTLKEKISNITQHLFLSINSNNVKEIQKSLIRLTELKVNYAQPLYPIYQHALEYCLSRQEQGEQVDHIIDTIIKFTTNEKSKINFTNMYFYNIVMGNENLVNIFQDHLDGKEKSFQFIITQNIIHLSLLYNRYDIALEWYLARVNRYKLPTFQNLNNLFRFYHKEKNDQLGEKFWTAGLVFRKGQSFLEKEWNHYRSLDPYKIVQDFQIFAGFQSFVHNTSPVIPIQLQKVYFDLEIAIKEKKSSIVKKILMRDIYPKKLIPPKTLLIDALILYIEANPKEFLKSTKVPEYIKPLMGISPLDLSDDIESGVQNLISCGNYSPSVSTHNSILQGLFNTENYEITCKYLIAMFENGVPITSLKMVSSKLVENYSNYKDYIKSLLPIIQSQTKSDGNTPSYASNLFLKHALEITNQMPKDQQKGALDKLLENYKRAPKDQFSMRVGIDILAKIHPVPPTENLDHWEQVFNDQVTRFKDSDSFYLQFLFEKLLSLNQEALVIDYVEKDPTCLTTSHPKTLLDYFETIQKDHQKVIDRFLILQYHPKKIEYPLKLAWIILKANKIIQNPKVKEIIEQVFENRSFAKIDKITQLSTSSLSYLNLIIKDMKKANKPQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1N9</accession>
    <name>A0A8J4Q1N9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Obg-like ATPase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_001549</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. YchF/OLA1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000037">
        <property type="protein sequence ID" value="KAF2077164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1N9"/>
    <dbReference type="OrthoDB" id="424823at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04867">
        <property type="entry name" value="TGS_YchF_OLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01900">
        <property type="entry name" value="YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.300:FF:000001">
        <property type="entry name" value="Ribosome-binding ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000001">
        <property type="entry name" value="Ribosome-binding ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.300">
        <property type="entry name" value="TGS-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00944">
        <property type="entry name" value="YchF_OLA1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004396">
        <property type="entry name" value="ATPase_YchF/OLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023192">
        <property type="entry name" value="TGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013029">
        <property type="entry name" value="YchF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041706">
        <property type="entry name" value="YchF_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00092">
        <property type="entry name" value="redox-regulated ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF18">
        <property type="entry name" value="OBG-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06071">
        <property type="entry name" value="YchF-GTPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="5">
        <location>
            <begin position="56"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="65"/>
            <end position="70"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2077164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="420" mass="46023" checksum="7F2FAA5892490B81" modified="2022-05-25" version="1">MIKQFSKIVNFKTTTTGCFNQCGSIRFFAAAPAKDAGNANEKVKASVFVKSRGSVKSVGIVGLPNIGKSTLFNALTSSNAAMAANFPFCTIDPNIGKVFVPDERLDLISKLLKTQSKVGTQLEFVDIAGLVKGAAEGEGLGNKFLGNIRNVSLILHLVRCFEDKDITHVSEEIDPVRDIETIEMELILSDLQSLEKIIQEAGKKKQSNPEIAQRIELATRIAKALEGGVPARDIEIDEKEWPAFQSLHLLTSKPTIYTCNVSEQDASTGNKYTEKVKDFSRSKGLEIDPVVVSAKIESELANMESEEEKKEFLDLYGLTNNGLSKVINGARGLLGLQSYYTASEVECRAWTIPIGTKARQAAGVIHTDFEKGFIKADTISYNDFIACKGDEKVAKEKGKQRQEGPNYVVADGDVMFFKFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1S9</accession>
    <name>A0A8J4Q1S9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine protease</fullName>
            <ecNumber evidence="6">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_001537</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase S1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="6"/>
    <dbReference type="EMBL" id="AJWJ01000037">
        <property type="protein sequence ID" value="KAF2077152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="7464126at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056681">
        <property type="entry name" value="DUF7779"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050966">
        <property type="entry name" value="Glutamyl_endopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008256">
        <property type="entry name" value="Peptidase_S1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15462">
        <property type="entry name" value="SERINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15462:SF8">
        <property type="entry name" value="SERINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25000">
        <property type="entry name" value="DUF7779"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00839">
        <property type="entry name" value="V8PROTEASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="NB-ARC" evidence="8">
        <location>
            <begin position="446"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7779" evidence="9">
        <location>
            <begin position="677"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="627"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2077152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1952" mass="223392" checksum="3071A2DE3BD9FDBD" modified="2022-05-25" version="1">MEVKVIENDGESLEFNSNSNEYEGYSKSSDSGLAHYTHCFKPHDLDDSLWPHSAHGRLIISCRDSKTLYGSGVIVGPNMILTAAHNLFDSSSSVGNWPESISFIANGDKTKQRYQSAVLIRHCDHRKDPFDVGLLILRDAIGFKFGWYGMLSTSLNGHLKCFKIMVTGYPAEDLHNTKKYKEYDITGKISVKANTMFSYNLSTKHGQSGGAIWKQCPSIIENNSSSVFVIGVHTKEGGLGTQLTYDIFKLLVQYIKKYHFKSYSQPLPYSNNNNNNDNNNNNKTIEWYYNQAKKYQQENKIEKELEYLQLAFKLSAQKTLIQDPEWIEDYANSLLKVYNASLELTKRSVYLSESFNLFQKLFEQNCSPELISRVQDKLSIICQQGLGGLDINVYGKNHASVQPKLPRVTYTKVIHKIPDLPTNLIPRNDHQDNVNYIEKIHKIFNNVSSCRITITGMGGIGKTTLAKQFAYNCFEKKLFSHIFFISSDSNENLVKDYKTILKFYLGIRELDSSDSDCIIRFTQAIESLPSSCSTLLIYDNVESKQFLLNKIPLDNAKIIITSRNRQTSGDPWDSMISLELLSLKESVQLFEKWLADFIDQQDRDQLENLSNILQRLPLAIAHASSYIQNQQLTIQEYINEFREFKEELEDQDDLEEQYKFQASYDRLIGKTFNMAKTKLSQPEKLILEYLAYLNPDQIKTSTLRIILDHKTDQKINISLGKLYNFSLITQLDKQGTLSTHRVVQHVSLRQQHRDSKIKKNLTFILNRFYQYLYDDQASRYGENNVAEYYADLSTISNHVSFIGSIVQLHCKEIKCAGILMKHQLIMNLNHQRLSIVHTDQSALEDQFEKFDFQHKTIDKKDIARLVYQVISRDCLENDYHITRYSNNYIYSLIKIISDSVSKNLLSLDRAKVVVVYIFDLSRYLDLKNISLVIDNFILSINNGTFTDIQIQDIVSLTKQLSNPLMDGFNISTIIQIVCCVLDSKQLSNEKIQFLIGQTLSLSTPTINGQQITSIIESLLQLIQSKNLSNSDIDRIVHFILCLTKPNVDGYNVSIITKTMVNLIKQNQLGISQIQEIVNHTLLLTGVNINGLEMSSILQSITNLYKDNNDKNIKPIIKHVGTIINHISTLDQVSFVIKVVSDLFKSNTLEQVESIIKKTFFIISQEMNGGEVGFIIRSIEKLVQEKDHETITSILDNTCLFSKDINARNISCIIHFFTEIIENNVERDIQTFIINHTLQFEIQYMDGNEVKLIMKAILSLYKKLNLSNDQILNIIKSTKQFVISNSGEQIATMIRSLSSLNLPILESPNTTTTNRGPALPSKTSNIFISPVIKSILISSKNNPNSIVKHTNALSGYISSIISLIVSKKTYLSEKQMTLTTNLCQVLIKNSMDIHEISTIIECISSLFSRHNLSSENIEQIVSFTQSLMKQEAMDGNQISSIINSLSSTFTRYKLSMYQTNKVFDYVRMVSNKMKSSSTLSSVVQSIVSLFGQGRLTHQQIQSSMDFTLSTLTSHMDGYDISSILNLFSHLVQKNDDNIYKIIKHFKTISNEFYGSNISSILHKISSLFKEIPQEKTLSKIISNTTELLSNKMDGQEIISIINTITTFYKSPPGSSTYIDCENIDRIIIQTKRFKNIDGYQISLVINAIALLYQHNIGDYQINNIINHALSLSFPNIKCYDSIIKNLFLIYSLEERNKIVSVCKLEMKPFINPATLLYNFNNDKLFKGVQEQDEIEFKQTHIKRIQEILGKVSKQTQQQLAIIQHEIYLSTSLFYCQDQLIEKIIDYTVILTTHLKLKETDILLIIKHLSIISTSFCSQQDLLECLIKSSIVFKDIIKNNYHQFYLILQSLVTSNSNSSNIQQQISIIINILKSLPNDIMQLLLDDSNSCFNLSNIIQRLLYFESNYKGFLQDLHQQLVTISQRDQSLTDEKWIHMVIYKIFDPKTIFNPSNEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1U0</accession>
    <name>A0A8J4Q1U0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cellular retinaldehyde-binding/triple function domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_000451</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the patellin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000008">
        <property type="protein sequence ID" value="KAF2078261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1U0"/>
    <dbReference type="OrthoDB" id="75724at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.680">
        <property type="entry name" value="GOLD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044834">
        <property type="entry name" value="PATL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45932:SF17">
        <property type="entry name" value="CELLULAR RETINALDEHYDE-BINDING_TRIPLE FUNCTION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45932">
        <property type="entry name" value="PATELLIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="11">
        <location>
            <begin position="104"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="12">
        <location>
            <begin position="238"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="369"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="369"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2078261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="407" mass="47002" checksum="83CC741DD9064E44" modified="2022-05-25" version="1">MSGYIQDLSENQSKSLATFKQQFDENIGKVKSELDSQSTLKIYGVDLESDSSQRDIIFLKFLRARDFKSDQSYQMLVDSLKWRKEFNTDNILNETFPDCYKTIGGIHKTDKDGRPVMVNYYNNIDVNTVFGNGVAEFLRFKVQQMEMAIQQLQATNWQVEDLVVIHDYKDVSMFRMDPRVKQASSQTIQTLQNNYPEFLFRKFFINVPYIFQALYSLFTVFSSERTKSKFNVLASEYRKSLLNYIAIENLPHELGGFQNNNDADFNSITTIELKPKSTHDIELGFLDTDKTVEWEFIVLDGGNDVNSQILLNNTKQCLSIKSKESNTAGSFQIEEDGKYSFMFDNSQLNQTKTIAYRILSKSKSIHLHTNNTSTTTTTTTTTNIDDKPTTPQQEEEEQKQEQDKVEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1V3</accession>
    <name>A0A8J4Q1V3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein SEY1 homolog</fullName>
            <ecNumber evidence="9">3.6.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_000280</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Probable GTP-binding protein that may be involved in cell development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Endoplasmic reticulum membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family. RHD3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="9"/>
    <dbReference type="EMBL" id="AJWJ01000005">
        <property type="protein sequence ID" value="KAF2078413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1V3"/>
    <dbReference type="OrthoDB" id="1597724at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016320">
        <property type="term" value="P:endoplasmic reticulum membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01851">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000727">
        <property type="entry name" value="Protein SEY1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03109">
        <property type="entry name" value="Sey1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008803">
        <property type="entry name" value="RHD3/Sey1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046758">
        <property type="entry name" value="Sey1/RHD3-like_3HB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45923">
        <property type="entry name" value="PROTEIN SEY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45923:SF2">
        <property type="entry name" value="PROTEIN SEY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05879">
        <property type="entry name" value="RHD3_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20428">
        <property type="entry name" value="Sey1_3HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="9">
        <location>
            <begin position="1"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="721"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="9">
        <location>
            <begin position="742"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="745"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="9">
        <location>
            <begin position="766"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="domain" description="GB1/RHD3-type G" evidence="11">
        <location>
            <begin position="60"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="70"/>
            <end position="77"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
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            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2078413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="815" mass="93775" checksum="5470B65198BB24D0" modified="2022-05-25" version="1">MSESETNSTPEGVVVEPSENSYTDIVQFIDHAGEIVKETDNEKKSFLSTISKREDFLTKGFDYSVISILGPQSSGKSTLLNLLFNTRFAIMEARTGRKQTTQGVWMGIGGESTGPETFLILDVEGTDGRERGEDEKAFERKTSLFSLALSSVLIINMWSHDVGRYNAANLSLLKTVFELNLQLFQKKRNHKILIFFIIRDQDNVTPLSELRRVLLEDIEKIWKELSKPSEFANSRETDYFDFDFTALPHKNYSPDQFKTQVDILRQRFLDPSLNDFIPKKKYRNEDVPADGLYQYSFNVWETIKSNRDLDLPSQKEMLALYRCDEFLESSFNQFTSEIKSTRESIEKGRLVNNFGDSSKKILDDAIKRYDEPSSRYHPETVAKKRQVLNDRILTDLKYLFDKQIEKLYEKTIEFYNSLVQEAVKPVNGGKSSSSSLKRSGATNQNGGNIVIIPQFNIWAEGTKKKSIEYFEQTAKQSIVPGSDWTFVEELNVLDEKMTKEISQLRENQLEKLSSLMKENTFQKLNPILLKITDQASDNMWQKIRAAYNDEAATNEKEYQSRLTNDFAIGESECEEYLNKFKEGVLENLKSKISDRAEFIPMKMRKRFEEHFNMDDKKLPRKWKKQDDIATIFQEARLNAEKLIDLFSYLRIDEQDSKTSYFSLLPNGEHEENTNTNEIEQDKVIIAYKQCMTMCENFRHDTKNDYMQALSEQARLTSAGGIPGYVLVLLCVLGLNEFIAIITNPLLLLLVILLGGFGFVLFKLGLTGPFIDYSSQVLVHIINKIKDIVLHVENISHHAVSNNNSNSTNGNKEKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2P7</accession>
    <name>A0A8J4Q2P7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Chromatin-remodeling ATPase INO80</fullName>
            <ecNumber evidence="11">3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_000993</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATPase component of the INO80 complex which remodels chromatin by shifting nucleosomes and is involved in DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the INO80 chromatin-remodeling complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The DBINO region is involved in binding to DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="11"/>
    <dbReference type="EMBL" id="AJWJ01000022">
        <property type="protein sequence ID" value="KAF2077677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="372624at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000006">
        <property type="entry name" value="Putative DNA helicase INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020838">
        <property type="entry name" value="DBINO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050520">
        <property type="entry name" value="INO80/SWR1_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685:SF2">
        <property type="entry name" value="CHROMATIN-REMODELING ATPASE INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685">
        <property type="entry name" value="HELICASE SRCAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13892">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51413">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DBINO" evidence="15">
        <location>
            <begin position="620"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="907"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="1496"/>
            <end position="1651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="260"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="481"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="572"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="697"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="745"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1675"/>
            <end position="1800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="58"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="101"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="125"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="178"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="270"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="283"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="306"/>
            <end position="316"/>
        </location>
    </feature>
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            <begin position="317"/>
            <end position="363"/>
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    </feature>
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        <location>
            <begin position="388"/>
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        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="12">
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            <end position="490"/>
        </location>
    </feature>
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    </feature>
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        <location>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
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        </location>
    </feature>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
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            <end position="1740"/>
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            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019805"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2077677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1800" mass="202656" checksum="79F0FD75ECE0D459" modified="2022-05-25" version="1">MYSPRSASSSPSSRYSSYSSSRGRSSSRSRSFSPLGTIDYISLPASSQKSMAYPPPNYHSNNSSNSNSNNSINPPSVVQTSTYPPSSSSSSSKYRSKSPSHRNSSSSKTSSSSKSTSNSGGGGSSRSSSSRYSSSSSSSKYGSSSKSTSTSSSSRSIGLSSSASAIPNSTSSKDKHSSSSSSTSLSASTNSSALLGLAKSSTTPRHSPSLRSSTSSTNLLNSVSGINSSNSKSNALKFKKISRKDKEKILELLNEHESDNDFDFSLSNSTKPSPVLKSTNYHSSSSSTSSSTSSLPSSSSILTSLQPLLNTDNTPPSVLNNNNSKNSNHTSSMSKSNINSLINNENINVGKENNNNNSSSNVSTYQNESRVPMDISKTNDTNNHHNSHSISIGNSNNQNNESSFSDPDITEDYYFKMLHEYKKDKSKKKKSISDKYFKDIKLSDDDDNFIFNERTDRKQLKLMKKLLDNENMRDRERYKVMKKKEKKKIPLSKSSKYIKDRIKSSRSKSSSSSSSTHLYSKKKAYSDDDDSSSGDDLQALKEHLSNNPITSEDEDHYDILYNKSYKKKKKYSSSSSASAGTLTNLSGSTSGVNYSSSSSNNTTAFLATRYPEVDQDFHSKWATLIRKELPRQYKKYQDNHTNGLANNKKIGSMMSREIKKRYKAGIPQPKPVKDIQNRSRRLVKEMGAYWRKFDKDEREAKKRAEKEETNQRKREEEIQEAKRQQRKLNFLITQTELYSHFMGKKLTDETGGPSAPSTSAISAQIDASNSKKITEAATGDGQQPQQVEEYEDESDDELNEQDKQEVEKLKNEAILKTQKAIEQQITKTQNFDKDVDKIRNSNPETMAEEKNANNLATDVLMDGNNNSNANINGSNIPFGFGIDSLSQPSILNAELKPYQLKGMSWIVNLYDQGINGILADEMGLGKTIQSIAVLAHLAEEKNIWGPFLIVTPKSTLHNWKNEFAKFVPSFKVLPYWGNQKQRQTIRKFWNPKKLYNRNSPFHVLITSYNVIVLDEKYFHRIRWQYMVLDEAHAIKSSASNRWKTLMSFNCRNRLLLTGTPIQNSMAELWALLHFIMPTLFDSHEEFAEWFSKDIENHAMSQGGLNEHQLNRLHMILKPFMLRRIKKDVENEMPPKYEVEVNCSLTVRQKKLYQGIKSKISISEILGNNSFSEASMKNLMNLVMQFRKVCNHPETFERSECKSPLLFQTAEEAVNVTLPLSTTYLKTVKAVVNNPITFSIPKLVYRNCWFIQNQSDSNINDQLKQCTINRFSIFSPSTSNDNNSITSISNLIGLSPQELFNLKNNFSILDMYITNQTLEKEVYPVLDYIYSDKDSGLSNTIKYLLLEPTFTCKLSPHRLKSNYQQLVISPSDRCQWNQVLIRSVFSLYPKCTAPPIDIVCSDQSFARDKAAQFLNDPEVQIPLTNLVFNGMTNNIDIDQKQFEFNQLDLPANLYHNTLDQFVDNAGLVGELNGIFGSSSIWVPSFSKSLNDSGKLQVLDSMLKRLKPQGHRVLVYSQFTKMMNILEDFMIYRKYKYRRLDGSSKLEDRRDLVDDYQTDNSIFVFLLSTRACGMGINLTAADTVIFYDSDWNPTADEQAMDRCHRLGQQRPVTVYRLITKGTIEEKILKRAKQKHQIQSIVIAGGKFEIDNLSTGDDINEREVVSFLLDDDEVEEKFKNQFDPITGKKRKEPESSETSVQDKPFSPSDIPTTYISKGRPPKEKEPTPVTAPKRKGPKPGFKRNKVDPTLATTPPTISNTSPKNEGDPEMPSLPKPKKPPGKPRGKKAAAQIKLQEQQQQLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4F0</accession>
    <name>A0A8J4Q4F0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000638</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000012">
        <property type="protein sequence ID" value="KAF2078087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4F0"/>
    <dbReference type="OrthoDB" id="3219396at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="139"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="301" mass="35347" checksum="2072A887C7FF3E8A" modified="2022-05-25" version="1">MGSSLSISKLKRRRSSSQYQYNNMYNFDNHENHEHTIVQTDTMSNNNNNSTSSNRLSPSSEREERNKAQLILYQYYEIPSKLIEFNLMNLNYCGSLTPLPSIPVDLSFYVRLLMAPTTEFHNNDSDSDNTLGDKDLLSSLLSSELQVKIVLELKFIDIINLSLINKYWYQLCKSNILWKSILKRDVRDWTNIELVKETKRALLKFNNTDIFSWKKYYCDNRRLRQCKHCKVVYRDTYNSKVSCQRHSKVRDIIDSASLHIVLPSGVYWLCCYNKSIDAPGCEMEQHQESNGFSYLKSANYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q585</accession>
    <name>A0A8J4Q585_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004504</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000160">
        <property type="protein sequence ID" value="KAF2074186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q585"/>
    <dbReference type="OrthoDB" id="10506336at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="290" mass="33296" checksum="32BBE905191C6645" modified="2022-05-25" version="1">MQGVNSTRLLVRLSGEYTQKCFYAKRMVSKRVYETLKREKVDWDSLKTPQLFMDLCKPPDNRPVDTLKPKIAKTIEGIEQYLSPNLGSPQSIDEEIKKSENMENDLMELIDKIKYHEVGNPAAAAAEEPAVDRYNNKKKKAPSARQIITQILNDSLDSDRQYQQQHHQVNRNNGANSTAENDTWVSLRDIDPEESNHPLNDNLSLVERMSIAKISTASDSINTSTKKSKPRFYYQAGEGSPLFNFSAYQLEEINKLEQKSIELQKEREEYASLNKSKSDFIKDMKNLKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q7D1</accession>
    <name>A0A8J4Q7D1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">CYY_002928</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. ROCO subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000087">
        <property type="protein sequence ID" value="KAF2075746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10252328at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1390">
        <property type="entry name" value="ROC domain from the Parkinson's disease-associated leucine-rich repeat kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032171">
        <property type="entry name" value="COR-A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057263">
        <property type="entry name" value="COR-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020859">
        <property type="entry name" value="ROC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051420">
        <property type="entry name" value="Ser_Thr_Kinases_DiverseReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48005">
        <property type="entry name" value="LEUCINE RICH REPEAT KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48005:SF13">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DDB_G0278509-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16095">
        <property type="entry name" value="COR-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25497">
        <property type="entry name" value="COR-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08477">
        <property type="entry name" value="Roc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51424">
        <property type="entry name" value="ROC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DH" evidence="16">
        <location>
            <begin position="202"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="15">
        <location>
            <begin position="493"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="Roc" evidence="18">
        <location>
            <begin position="1175"/>
            <end position="1392"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="1947"/>
            <end position="2211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="111"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="845"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1532"/>
            <end position="1555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1594"/>
            <end position="1614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1767"/>
            <end position="1814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="2214"/>
            <end position="2504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="111"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="155"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="182"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="852"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1595"/>
            <end position="1614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1778"/>
            <end position="1806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="2220"/>
            <end position="2230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="2239"/>
            <end position="2257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="2264"/>
            <end position="2277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="2294"/>
            <end position="2320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="2384"/>
            <end position="2396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="2399"/>
            <end position="2415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="2450"/>
            <end position="2464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="2470"/>
            <end position="2492"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="1974"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF2075746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="2504" mass="275683" checksum="9780D0DB0ABAFADF" modified="2022-05-25" version="1">MDEKKKDKKKDKEQKKKDKELKEKEKKDNKEKEKEREKEREKEKDDKKSGLLSSIGGWVGDKLNTPTTPTSNPLPPLPPIPSASTPVLSTQATQENHKKAMEFWTTTTASTPTLNISNSSLNTTNPSSGSSNSSSTSSNNNSSSSNSLNVKEKTTLGNSGGNIPTKESSPTNSKRNVDNSKDSSSNNNSTNNIDPNGAIDENRKKLVSNLAQFLASYSEAIKVTIDVFYSPLKKNDNILSHDEVKSIFSAIEPIHGVNHVINEDFKKALNNWNPTTQASILSTFAQFIGFLKLYKVYALQYNYSLSMLCSLAFNNTRFDEFVKKGEQKLVDLNYKLAGIVTLPNNSSSMKPVSLSEILGTQYENTVTTTTTTSSSTFSVTGLRKVTNVSGGGTNSVSGKFTQEYTYSTLPSLLLLPIHFLARFHQFFKTLVDSIAVLSQDYKPYQKLYKQIGVVVREIVNESLNINKVISISKSIKSPTIGLFNSTEIIQSRRYLKEGTLIEQFNNQRTTYYTFLFTDIMLFTEKIEDSSTPTATFIPYEGSLYLLKKLERISSIQIDDVELGFEYRKGFQLKSKDNSIFYMTSSEKEKSNWFQAISQAVHKHISSGVGSSLASNNNSSSSLDDDDVEEEDLKDIGGLVKCVANGSRSKIELSPAILKSSTDPKPLFTALASSQIVNHLVFTPLSMNDKVMQMTLSMMSINKSIGRLCLSQNSINDNCAAALGDMLRYNHTLMTLELSDNSISDKGFIALVDGILSHPSISIVSFTLNQVTDVGAKHISKLIRFNQTLNALYMDDNPISTPLANELMDLWVQTQNTVMSLLVFSNITPEYNEKIKNKIKSIKSRLEKKKKTPSATASTASTGSSTSPQPASNTNKAITTTTTPSTGKGNLLDLSYLDYPEITVQLLNKLNMLNLDNRRIGDLKELYLDHNCINNVPANVFRELKNLSILDLSNNQLSSIPIEVSELKELKVLNLSHNNLTNLPNEIGQLVKLTHLDIGFNFIETINTEAISNLVNLRILMMQRNYFNRLPTSLFAKLPLLESFSISGSPCFHPLKQRVYEAIATRATKVDFSDSGVTCLPIEIGSIANLVELDLSNNRIKDLPPQIGKLVNLTSLNISHNLIEQLPWQISKLEKLKSLSISGNPLSFDVKLNLPDVMAGEDLNALLKYLSFSTSRDQSCMRMKLMLVGQENVGKTSIAKCLKKEIIPVSKKFRQTIGLGTKKSKTPTLAESEAFTPSGAINPLNTTLNISTDGINMDDWRPPTEDQSPPVTFSIWDFAGQEVYYSTHQFFISSRSVFIVVFDMSLYNPDETSRVPYWLQCIEAFGGSSPIILVGTHLDELPSGTEISQITADIHNKYFTKFPNLKLFLPVSCKSGKNINKLQNHIVKLGKSEKKLGDLYSRAYFQLESLILSEREMNSPPIITFQEFSEMAISCGIPSTSIPHAADFLKELGIIVYFDDLKSGLDQFIFIDPPWLTRLMATIITSKPNFVQSGVLEQNNLHQIWKAPDFPQYLHHVLLAILQKFEIIHPLPDPKSVNKTPIPPTPSSSKPSNSLVSRTKSNFLNAVSATGGASGSGGSLSKGSSLNIVKKLEQATNTSNNSGSSSSSSNSTDSSLVPKISTKYLVPVLLSEDRLNSIEKTLDEARKQNNGGILERIYQFEFIPVGLFSKLMIRTMHFTTVKEYWKHGLLVSREDAQCLIESQSHLNQINIKSWGKNASNLLRFIVETAEVLIDGWYKLRHQFLVPCNCAHCTSAYLNTDHMTIGSSVGGNLMTQSRDSAGSSSSPTSSALSSSSNNIGNSGLSSTTGDPNSTADTISEADLDFTDYFDGDSVSPGGTLKSKRKKNPTKFLTLYKTKQKSSKEKQPVNLSQISNLINQTLSMNLGNKYDPRTLFLYEDIERSFLSKKYEMLCRSPYTGEETMVPLGSMVPELVMSDIGPNFTLEYKDLEIIEQIGEGGFGIVYKAKLRGQIVAVKQIALTGNSQANAEDIYREFRREVWLSNTLTHKSIVSLKGYCLDPCCIVMEFISNGTLYGYLRKGLTIPWSLKVKIALNIADAIKYLHGFNPKICHRDLKSPNILMLSDLSDGAEVVCKVSDFGETRAVVTSALGRDKLSNPIWLSPEIMRGEEYTEKADVYSFGIILWEILTGLLPFDEFPVAHSSFMYQLEDEIMNGLRPTIPPDSNQDYTNLIKDCWQNDPLKRPAFPEIYQRLLNMQPNGGITNNSNTNSNTSRPKASTIFSNVNNINSSNSNNNNSSVSTPTPLKPVNSTPSVTPSSNSFEKPNPPLVRKLTQNFNQPTNSSANNNTSTPTITTTTTSHTPSQPKPPVVKTLPITPSPIVGKPPATHSPTLTAVPASKPPPRQLPKTPSTMGLLDRSASASSLNYTPSSSTPNHTSPPAKASSSVILKPSPSQSNMSKPLPKVPGVGGSTAAPAHPIVPKLNSPLVASSKVSLISPSSASTGSSTSHPLKPSASTTSIPTTQLNSSLLRPTGSQLFKKPPASNPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q9W1</accession>
    <name>A0A8J4Q9W1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000997</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000022">
        <property type="protein sequence ID" value="KAF2077681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q9W1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34078">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34078:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="130"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="165" mass="19053" checksum="57915B3C314113F8" modified="2022-05-25" version="1">MVYENQQPQPQQIQDTPQQVYYPPGYMPYIPPNNTDQRGNWQQPTSQSHIEYPAQAQYPQFQQQYAPQQVVYAPQPVSYPPPVTATPQKSGDDSEHLIGSVLIFIFGWMFFIPWVFGFLYIRSRNITAKILAIISIVMFFLTLMVLILILPIIFAVGSKHDSNDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4QA16</accession>
    <name>A0A8J4QA16_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000892</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000019">
        <property type="protein sequence ID" value="KAF2077771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4QA16"/>
    <dbReference type="OrthoDB" id="9449012at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032039">
        <property type="term" value="C:integrator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034472">
        <property type="term" value="P:snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00198">
        <property type="entry name" value="vWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000010">
        <property type="entry name" value="Integrator complex subunit 6 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057413">
        <property type="entry name" value="Beta-barrel_INTS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051113">
        <property type="entry name" value="Integrator_subunit6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12957">
        <property type="entry name" value="DEAD/H BOX POLYPEPTIDE 26/DICE1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12957:SF2">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25462">
        <property type="entry name" value="Beta-barrel_INTS6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="2"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="458"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="621"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="795"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="468"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="621"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="691"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="743"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="804"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="831"/>
            <end position="843"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="965" mass="108495" checksum="BC283AFEB3F99728" modified="2022-05-25" version="1">MFITFVVDTSGSMSQKTINGMTLLDCSKAAIEHFIKIRSKDSSMRNDRFFLITSEENNINAVKIGWKDNFNTFLQEVKNLSSKDMSNVGFSIQKAFDLLNQFRIQTSIDNYCQGRNPWFVDPAIIILLTDGSSLTNYNHILENFSLPKSPYNANSDPTMEPFRWDQRLFSIVLKFNSIPSSNPMPLPLEPAIAPMCDITGGKCQIATNMKSMLQQVEGLIQKLQVGVVVSFEPLQSQQHQYQQQQQQQQQQQQIPPSLPSSLHKMIYVRTQGGFWPIPESYYPDMSNMNLPVRSAHPVIRYSLVEIDPHIPESFPFDKYELESCPLVQYILANKINCIPVFIANSSQLPGQGEPFGYLRANSTGNSVNLFVFPYNFPRLWDLVDDLAQNQYKMIPSQKWKQDFDSYLLSVPPYYIHPLRSAFKRICNINLIPDSFDGPFLNFVNNMIKKLKSQSQSKLESERIINSKQQQQQQQQTQQQKDTLSSSASSSMSLLLPTASSASASSTNNSIFKSTFTKGRGFHQILDSGSTGGSSDVVMDTGEENIDYFGSDTGAPSSTNTVSLDPQIQKNVFDISRTSLLNQLDKMRDHILKKQVVDDESKHNLPISQMGNYHDTISKRESLRDIDDDKKPNHALFGNPFRKEKGPRFGVSIDEADEGSSLEGSDNSKKSPFGSIGKRRRLTRAYPPSTSPPQSNTSSSGSSTTTTPSVASNTNTNTNISNGPSLMDTGSSLPNPQPTDEVDQQQQQQQQQQQQQEQNYQEQHQQNEPKEVQPEPTISWSPIELSAIKSTDVVMTDTPIPTPQIPTTSIPSTQQQPVQSNIPQPPQPPPTATTTTTTQPLQQQFKPSPVVSNISTKPQSPPINSINNNTSTTMMMNGNHKMKRSTSIPNHEIIVGDSNIIKFILKEIRKPIKNNHDIIFNQLKYLSNETDKSVKLFNLNTILNLAQEYKKYSLIVKLKNYIDQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UP85</accession>
    <name>A0A8J4UP85_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009826</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000821">
        <property type="protein sequence ID" value="KAF2068851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="19433at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="614"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="domain" description="RNase H type-1" evidence="2">
        <location>
            <begin position="1242"/>
            <end position="1384"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1656" mass="191076" checksum="1DA202931D7D9981" modified="2022-05-25" version="1">MEILKDKTKSPDIIALQEIKSTKIDLTKLERITKDQYNVFSSLSPVANKAGVSTLVKKSIQHASIKAMDDEGRLVAIQLHLPNMPKDFIHVNIYAPVDNNAKKKFWENDLPTFISKLHNNVNRTPIIIISGDMNNTLTGNDKLHMDTDHPVRQAHNPGGTEHYHNFLRAAGYVDIFRELHPDKYEFTRTDWPNNSLSRLDTHLSRNLDTNLVYNIGSNVINWIVSDHSMVTMELNLKRAIFDHIPHRSRSRQEHDQKREILNTCTKEDFENFSITIEPSLKELWNATFENRPTPIPDINQLESIIKEFNKCLIQSGKKMLNWKTVGRKRETPISDEKLELLKVVNMATLANQIKQLLMNLKVKLHLLSTTEDAPTRTQLLNDYELYMRKLLSRFKSNKNTHQSMIEKTQTEIQRLNNTPIENLDHTLINTRIEASINAMNEDLIGIRRTRDNIKTAINRAIHKEWATRLQELGDKRDKGFYRLAKYNPALTANIQPQFLYVGNPDNPDTRTVIHCKEEILQETEKYFTNLLGESSYTIREPNQEPWHQEALEVLATPTLDSLAALQDTINLDEIKTAIQECSNGKATGTDEIYNEYLKHLPKVGLELLERILNGCKQLKKIPKQWKEGTIFTIYKDGSPNELKNYRGITLLSNIYKIYTSILNKRIHKYLDATNPLSETQFGFRETRSTITPLQVIGNIFSDARKFDKDIHVLYLDIAKAFDEVPHKGLIHALKLKRLPEEIIEILVDTLTDCTVKVITTKGLTKNIKIGKGIKQGCPLSPLLWNIFIDPTLHQINRRSLGYSIPDEPPQQISNLVFADDFAGIANTLAKLQEIADIFTDYLDFYGLKINVTKSAYTYNIRIQANNSNSNPNQTTYQIKYKNSTIPTKKKSESYKYLGAWINLELNWKDHKNYIERKFSATLKNLERKGLYPDQLTDCINSLIIPVITYGCPVVNYDNGTITKWTEQLAKAVKNAARFFNNTNLDIIYLNKENLGFGLFDLKKTLEHTTLHTILKALHLADQSYKTYVQLWDKLQFESELKDEIKYKNNLAVCLDGLKSTNTYAFFESLHENNLNIIFNADPLNIKSKANFVKFYDHKIKNARNKAKSIRRILQVNKEMKLEDLCRLNTTVNEYQLKEQNELTNILSEDWDDDKYLRKKFCINGNSRKIKNEILNLLPSDWLRDTTQSLPNQIPNTTRDIFVKSVKVKDDNNDKTTTIDLNQQYTLLNHHHLKDHKNYINQDLDEYVIYTDGSVQNDGSAGYGIYVDLPVEKVGIALNESLIKTINNVELLAILNALKIVPTNKKVTIKSDSAVSISIWKALDYSNNPLLFFGKAFYNTVLELRKIKNERDKHGKGNTIIEKVKAHMGVMGNELADYLANKGTKEESYCAISPLIYITEKENKVVPSTRVHTKQQTQILHKKALAKLEHQGEIYANGEQFIDKKQYLEVQSEIPMNQRTMLKKMILNNGPWKKTMFNMNHENVPDDKCRDCITRYEQIEDIKHILFCDYEEEARSNLNEQLAGLLKAKIGTTSCNTRYKTIESFNWWTNLHDTRDIQNPIFNTRDKYRDIIAAMGIPTIGLLNFLKTEIGLAEGAAKELIKTCSLIIIDFNFNNILQRIERRRAEELIEIERRKHTEEHALRDATDTTFGPLSFLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPN5</accession>
    <name>A0A8J4UPN5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009211</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000656">
        <property type="protein sequence ID" value="KAF2069466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UPN5"/>
    <dbReference type="OrthoDB" id="10394449at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="524" mass="59032" checksum="909989951B75A036" modified="2022-05-25" version="1">MIDTVFYSVWRNCFLRGIIRNKVCQHLLINVSTITELHNNHQYLSLFSEKDKIDNSIYIRLNISDRDEFNQYINSPYKSLANDLKFNDSYTKEGEKSNYIGLDCDQIHKGVARLSCWIDETTSGTSSSLPQSLTELKVYSRHRFSNSFLDSVLSNLPASLRKLELPSDYSITTQVELSHTLVELDYNTTGSNIKKLIVPSNKIFTNCRVTVESIDQLQWIHSHPFVGILRMDQLPIETITCGVIPENILSLDITHHGDVEDGAFPNSIVKLAYRSNSPINKITLPQSLTYLQLDSLSQPLEKGWLPSTLETLQIFNYDLPLLKGVLPDGLKQLILTTFNQQLDTGVIPNSLEDLTLASFNNELKPFVLPEGLLVLDLSEFTGSFVANSLPSSLNSLELGEFEGSFEYAPPMNNLSVLIVNQVNSSAIRVISNTCNLRISCRSIEKGLNLQDTAIQHLQLLLFTSNRLSLSPNLVPKDIQTLRLYRIDIKSSGLIPVSCKKLETDNIDLDLNLIPKSTKHTFNNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPS1</accession>
    <name>A0A8J4UPS1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000629">
        <property type="protein sequence ID" value="KAF2069616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UPS1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="96" mass="10052" checksum="B00C21FE44D254E6" modified="2022-05-25" version="1">MTTIRSTFAFVNRTIKSCKSTNISSRVFVATTTQSTTFNTTPTTSSTTTTTGLRSSTTTTGSSLLNNKSNRGISNNIVGGLNSLIQIGSFGDDDGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UR60</accession>
    <name>A0A8J4UR60_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">sphinganine-1-phosphate aldolase</fullName>
            <ecNumber evidence="14">4.1.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Sphingosine-1-phosphate aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">CYY_007031</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 16 17">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.27" evidence="14"/>
    <dbReference type="EMBL" id="AJWJ01000353">
        <property type="protein sequence ID" value="KAF2071656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UR60"/>
    <dbReference type="OrthoDB" id="10254570at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008117">
        <property type="term" value="F:sphinganine-1-phosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030149">
        <property type="term" value="P:sphingolipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000020">
        <property type="entry name" value="sphingosine-1-phosphate lyase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.140.2150:FF:000001">
        <property type="entry name" value="Sphingosine-1-phosphate lyase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050477">
        <property type="entry name" value="GrpII_AminoAcid_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42735">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42735:SF6">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE LYASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 17" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16 17" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="16">
        <location>
            <position position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF2071656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="531" mass="58595" checksum="FF7F25C616929D05" modified="2022-05-25" version="1">MRLLEYTNDYLKDYQPAKLVLATAGITAATIVAFNTISDKDNRDRIVKKLFSGVKSLPMVQQQVKKERSKIKESVIKSFKTDTSNPHFVLPLQGVDHTSLLAEMHNLMKVDESNWIDCKVSGCVYLGEEEHSKLLNEAYSMFSLSNPLHPSVFPSIRKFETETISMVANMLNAHSKVVGSLTSGGTESIFMAVKAYRDFYKFKTSTPEIVVPVTIHAAFDKACEYLKIKVKHIPVGKDYKVDIEKLRKAITPNTILIAGSAVNFPHGIIDDIPTLAKIAQQHDIGLHVDACLGGFVLPFAEELGYDIPVFDFRIKGVTSMSIDTHKFGYATKGTSVVLFGSKKLRRAMYFTITDWPGGIYASPTLPGSRPGGLVAACWASLVAFGRSGFRDKVKGIMETTKNIITGIKTEIPDVEIIGDPKAMVIAFTCNNIFFVNDAMSKRGWHLNALQRPNSLHICVTAKLIGMENEFIKDLKESVQETQSLKSSDLPKDGMAPIYGTAHSIPDREMVGTILADFIDALITSDSKPNST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4URG1</accession>
    <name>A0A8J4URG1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006527</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000306">
        <property type="protein sequence ID" value="KAF2072151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4URG1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550:SF11">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2072151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="674" mass="79523" checksum="6A8C810E7DEE4FFD" modified="2022-05-25" version="1">MNKDLYQSVFGNIFLSTKVYDHVHQSHADKYPLRYDDIVDVGWMIKYGHVGLLREKIKNNSHLYVKPKDLFSIVAKKDTQIFIYLFERYKYYALSSYDVLMKWMLQTMVNNDVIKYLFQNGYAREIDDETVSLLDSDLVAWLLEFGHIEPSQQVVNELALNRRAKETRERQEKSALNNALLLDINKPTFKVPLDTEIWLSWQDQGEEMFFKMWDSLSSRIRVTDDLIYFHISRDLTDEEDDDDLEYDANVLNIAQHILEAACKIGSVKVIEFIVKKGYFLEVDSMISEHSHDFFKKLINKTLSRDEYKIIEKLTSVHIKKRDQDQDDYQDEDEDEDDYDYQFEGFIDKFEFLQACCQYGDAEKFNYFYTRFKIYLDLEYDGAQLFRDCLESGNNHMIRVLYSHNLFFHPQDKEVFLSLISQFSLGYLLEDVERTIAKDNSVYPTLLCRAIQCNDLFGVKYIFSKHKFDSLDFKIILQISSCQNLAIIDYINKNKSLCFTGQGLANKDDLFHGISLPRLSKLCTSNLPLAEYLIRENCYDISKLTPKQLPFVQLFFGNDHTITKLYGFRQMVYLFDHNNYSSIDPFINLLVSDSENWLKYFEYIYRNQHKFQSKPSFQSILDHIVANLNEQQNSRNQVQESKNQVDALLHKYGCVLNDGQHEHLVQHNNQSSLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4US65</accession>
    <name>A0A8J4US65_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_005458</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000218">
        <property type="protein sequence ID" value="KAF2073231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4US65"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20617">
        <property type="entry name" value="CYP1_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000078">
        <property type="entry name" value="Probable cytochrome P450 515A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24303:SF8">
        <property type="entry name" value="CYTOCHROME P450 513D1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24303">
        <property type="entry name" value="HEME-BINDING MONOOXYGENASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12 13" id="KW-0349">Heme</keyword>
    <keyword evidence="9 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="437"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2073231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="490" mass="56380" checksum="4C25E8865747C51B" modified="2022-05-25" version="1">MFLLIIYPILVIIILFLWSQFFSKNGRSSGNKISPMHYKTIPSPKGLVPIFGHLLQLKKNPHEKIKEWYEEHGNIFKIKFGSVDTIVLTEYPVIKEAFIENGPAFVQRFVRRSRTDLNKGVNLANSNGEYWKNLKTILLTEMTNSKLKRLEHYIQQEAEGVSDYFASVAKSGEIQDPANYIKLYSMNVILKFLFGIHYPYTVEGHSDHMLATIRRYFRSTGQPFPGDFIPLLYPFTKDKPKEYFQDYATIREFVSEIAKTRLDTMDMTLEPNNILDSLLIQHKKVGDIPLYGVIATCIDLVLAGSDTTGNSLLFIIIALCNNPEIQETLYEELVATFGVDQQVSYSDKSKTPYLCAIIKEAYRRFPAAPLSVPHYLDRDVEFRGYLLPKGSQVFQNVYSTHLSEKEWDRPMEFKPERFLEQQTSKPIIPFGLGSRSCVGMNLAENEIYILAATLFRKYKFMRPTSEKLSEDGIFGLALQSPDFKIKVEQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4USN7</accession>
    <name>A0A8J4USN7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase C39-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004858</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000180">
        <property type="protein sequence ID" value="KAF2073831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4USN7"/>
    <dbReference type="OrthoDB" id="15857at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039564">
        <property type="entry name" value="Peptidase_C39-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13529">
        <property type="entry name" value="Peptidase_C39_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035169819" description="Peptidase C39-like domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C39-like" evidence="2">
        <location>
            <begin position="28"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="343" mass="38444" checksum="F2BD3C3D05F592BD" modified="2022-05-25" version="1">MIKSSLLLLVTIVVIAINAIPTAYQIQGIPYHRQVTEYNCGDASMEMVFGHLNREIDQYQLDDIARTSDTDGTSSYDIVRSFQFSVMSSSMGLDEKDHEVKYGFKKYPLGLLSIGFASSDFWIDELKSMIALDIPVITLMAYLPSDRGGHFRVIYGYDDESQTIYSLDPWDRQGQPRLYNMSYSLFENLWNYTEPQSPRGLPFFGAMAWSLPVQGQASTNGNQSSISYSFNYVNPFPFDNVNTSLPTPLQALTTITPLSKDILIMKPSSGISTPGSQVIQNKELNFLFEVECVAPTGCKGLEVQITTSILVTGSLPYTNYKQTTFYLGYNYNDMLGETIVITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4USV3</accession>
    <name>A0A8J4USV3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Lipoprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004655</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000168">
        <property type="protein sequence ID" value="KAF2074041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4USV3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035172799" description="Lipoprotein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="16531" checksum="148FADCA77757633" modified="2022-05-25" version="1">MMKYSLFLLFALVLSANCISFVEKNACLFQLSVNVEGKDQSIVGFKSGNDLALDIDFNPSSMNLDISFKIPQGQDPTEATIIGLDVNGNQVQEFSLGFKECLNGGSSSSTTSWITGTTTSRDSGSTVTTSTSGTGSSTTSSSSSGFTGFFTSTFTTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UUJ0</accession>
    <name>A0A8J4UUJ0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PA14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_003167</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prespore-cell-inducing factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000096">
        <property type="protein sequence ID" value="KAF2075526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UUJ0"/>
    <dbReference type="OrthoDB" id="17079at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011874">
        <property type="entry name" value="Fibro_Slime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011658">
        <property type="entry name" value="PA14_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051154">
        <property type="entry name" value="Prespore-cell_inducing_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001673">
        <property type="entry name" value="S_mold_repeat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02148">
        <property type="entry name" value="Fibro_Slime"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31137">
        <property type="entry name" value="PROTEIN PSIB-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31137:SF5">
        <property type="entry name" value="PROTEIN PSIQ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00526">
        <property type="entry name" value="Dicty_CTDC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07691">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00758">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035309088" description="PA14 domain-containing protein" evidence="5">
        <location>
            <begin position="26"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="domain" description="PA14" evidence="6">
        <location>
            <begin position="111"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="410"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="537"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="410"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="479"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="661" mass="70779" checksum="6E2E8012EFEDCC5A" modified="2022-05-25" version="1">MLSKYKQISVLLLILFFYNIQDASSQATLELSGIIRDTTPSRNPDFEWNIVTAQGIVLSTLGADHTPTYCCGDNSYPPIMPVVHNQTTFYAFFHDVPDVNIKIPYTLTLTPDPNNPNVYTYTNLAFFPIDGQGFDNPTNYPNETVYRDANGTPHNFHFCFEVHTQFEYQPGYVFNFVGDDDVWVFINNQLVVDLGGIHRALNVSLNVDTLGLTIGQSYPFDFFYCERHTTKSEISISTSLPFICPFFDGCGVCQGDNSSCCLPSNCDNKPGLDLVCLTQTCTDNMTCTASQLRCNDNDPCTVDNCFFGAGCSFSPYNCRDNNPCTYDYCSPTNFCVFVPIGGCVACENTVCQSDDLCIPLECHPLNGSTCISNPVICDDDNPCTMDSCYQGQCIFTVITDCGTNTTTTTTTTTDATSTTAPATTAPATTAPATTAPATTAPATTAPATTAPATTASATTGPDTTTTTTTSSTTGGGTGTIPTTTSTSSTTTTTTTTSTIGSAPPPCVCPTGYDCRLEHHPTVGWLILCTLQNTTSTTSTSTTSLPTTTTTRPATTGQVSTTVGSPPTTISSTSTTGSATTMTSTTGATTMPPPNPCLYSNVRCRRNQVCSNNYGVPLCIQNGCLTCEDLGCHKQGLFCKFVRNRLFSRDPDCCEYIPTCSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UUW0</accession>
    <name>A0A8J4UUW0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myotubularin phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_002732</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000078">
        <property type="protein sequence ID" value="KAF2075976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UUW0"/>
    <dbReference type="OrthoDB" id="271628at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF112">
        <property type="entry name" value="MYOTUBULARIN-RELATED PROTEIN DDB_G0290005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myotubularin phosphatase" evidence="4">
        <location>
            <begin position="222"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="98"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="684"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="839"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="102"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="684"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="709"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="726"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="744"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="840"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="868"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="913"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="924"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="958"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="992"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1028"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="1">
        <location>
            <position position="437"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="437"/>
            <end position="443"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2075976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1068" mass="121302" checksum="DD035B7366A8ABD6" modified="2022-05-25" version="1">MNIDDFEYPLSDEDQSFDRPQPIIVEGFSLLPGEIVLLATPNVVNLSLISTNHRIGTMYLTNFQFFFIDDSTHQAVISIPNGTVSEIKKLKGHVTVKYQDQNKNTDTNNNNIDTSNTKNNENNKINESTVNNNNNPLAKSGRNPIPSSNNDPYWYADGISEKALILEIRCKDFNIARFCLPHHEKGLECFNTLHRLVFVDNPSSFFSQSYSPFKGGQNGYNGWTIYDPIEEYARQGLLSQGSEWRLSKINQKFELCQTYPQMLMIPFSISDYLLNKSALYRNKNRFPVCTWRHKYTHATLCRSSQPAPGISRSRCEEDELLVQAIRKSTPVSTSSTSTSPLYILDVRSKSSVGFSDTSKDISNYTNCVLEYGGLPSPQELRESAQKLNKTIRTWDDKKSWPDVQNSGWLSQISKLLATAKKILNYIHVEGCSVLVQCQDGWDRTSQLTCIVQLLADPYYRTIKGFIVLICKEWLSFGHKFMTRTGSLTSLPSKQTSPLFLQYIDSVWQLTRQFPTSFEFSDKFLQVLLHHLYSNLFGTFLYDSEKDRLTQKIPNETESLWTFLYGCASNGIYLNGLYVKPANLDEAPPASFYLTSPRVGSDLSSSGGTSTANIEPRERSNSFIVKKNCEDANVLFPNIQGVQLWTDYYLKWRTPMRASRKPLTLVEHALGTPLVNGDLLFIQKKRRSRRSSKRPSKSTSTDSVKDGSKSSKKHHHSHHHSSKSKRFSKEKESLDIKIPESNIDPIKEETIETKPGEMDKQNLEVEQEHISTSKVDEVDLKNIIEDERDILILSSNPSSSSRNNPLLNVLEPDLTMSNIPTIEEEPEPNENGDGEVFVKAEQSQEQENEHVQSPNTQEDSGEPLVTVPSLLNIQENNSRRGTLCTSNSSPCLTSPDGIDNNSHLTTTTTTTTTSHLNNGQNSPQESKEQRRLEKEKRKLEKEERRKERQERKEKKREKKEKEEKEHPKPDITVVLQNPSKAKPISLTMPARGSKNSRISIFSSTSSPQHTSTNNLHHHHHTNSPTNPLSPTLESETLHSADQPQLTESRDNLKKSRFFKSFSMRKAQPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UW62</accession>
    <name>A0A8J4UW62_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009309</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000676">
        <property type="protein sequence ID" value="KAF2069375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UW62"/>
    <dbReference type="OrthoDB" id="809632at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05288">
        <property type="entry name" value="PGDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000121">
        <property type="entry name" value="Prostaglandin reductase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041694">
        <property type="entry name" value="ADH_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045010">
        <property type="entry name" value="MDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205:SF7">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16884">
        <property type="entry name" value="ADH_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="2">
        <location>
            <begin position="23"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="346" mass="37349" checksum="5FE15B89685D0E1A" modified="2022-05-25" version="1">MTSQNRKVNRRVLLASRPVGAPTADNFKMDEQPIPKPGEGEVLLRTLYLSLDPYMRGRMSDAASYAAPTPLGSVMGGGTVAIVEVSNNSSFAVGDLVESYVGWQDYGISNGKELVKLKGVELKHPSYAVGVMGMPGFTAYQGLIDIGNPQPGETVVVAAATGAVGSVVGQVAKLKGCHVVGIAGGPEKCKYAVEKLGFDKCIDHKSDNLKQQLQDACPKGIDVYFENVGGKVFEAVFPLLNTKARVPVCGLISAYNATSINHSEIALAQFQGAILKKRMRVQGFIIFQDYFIFYDEFKKVMGKWVEDGKIFFKEDVVEGLENSQNAFIGLLEGKNFGKLVIHVGDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UW70</accession>
    <name>A0A8J4UW70_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNAJ heat shock N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009379</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000694">
        <property type="protein sequence ID" value="KAF2069298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UW70"/>
    <dbReference type="OrthoDB" id="1717591at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031982">
        <property type="term" value="C:vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072318">
        <property type="term" value="P:clathrin coat disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23172">
        <property type="entry name" value="AUXILIN/CYCLIN G-ASSOCIATED KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23172:SF19">
        <property type="entry name" value="J DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="365"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="366"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="405"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="443"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="547"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="688" mass="78496" checksum="F7BB9E53B8563581" modified="2022-05-25" version="1">MESFTEKMFDFQRRATQAVLQKMGAADTTVDEQFDNTLSNFKRLGEFLHHMEEQTQQSLTNMEKMCYTNNALASSLCNFVFSELSNDDNEFSTGLLSKFFLKTSAQTNKTPAENTNEFNQRQSTFQESVQRLYDSLGTMGHENFKTTQESLGFQVLNPIKKDEDVNRVTKQLLEKRKKLILEFDSLKRTNDFDKIRSAKEEFETMSKKTNAHLQNRSFIRCKLIVHTIVSMIQQFCLFFESNAILLEGLASFIEPLVGAYQLSPDQLSEIYSAKKPTSNVTSPIQNNSPIESRSPNFPSTPPPTYKDHIDQHNSNINSEKRNNSFENDFSKMSFTNGQRASPSPPTSTNSTQKEAKLFSVWDEEANNTGSDDSTNNSNSATKNNNNTSNTTPTKKQNADDDWMYNSSSTPNNSNNKSTANMNTSSDTWEDPLIPESTPKQQYFNTSTSSFNHNSGNFSRPPSQPQTPQHNNNHYPQQQQPQTPNNNNYPQQQQPQTPNNNNYPQQPQTPNNQYPQQQQQQQSQQKPKSGFENIFGDFDFTPPPQQRNTANFTTGGNNNNNPNFNNQPFNPTHVSPHVDHSQHQRSLIEPIVSEKIKNWGEKHGKKNNLRVLLSTLHEVLWEESGWEKVTYGAVMTPAQVKKVYRKAIMVVHPDKVHLGTPEQKMLAQRIFESLREGFDVFKVDLEPGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWE1</accession>
    <name>A0A8J4UWE1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009191</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000652">
        <property type="protein sequence ID" value="KAF2069493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWE1"/>
    <dbReference type="OrthoDB" id="10678520at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="413"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="413"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="430"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="799" mass="92750" checksum="560B90048127F814" modified="2022-05-25" version="1">MKKNLKPSDSEDQNDQRSKRMKISNPNNNNNNNINNNYKKIMDSNSSFFYIWRNSYLRNRINGIKMANLVISMSPDYLNQNISFVSKVFRDPEYNIKFKITIDQGTNLKSLIDCQHLSIYSHCAFSLPTIPPNFIPSHITFLDITGNLAELAMGSLPESLEKLLIRQIFTQDIEPGLLPSRLLWLSINDFRGRIFPGGLPPMLTFLKFSESFDQEIEQKIFPDSLTYLNLGNFFRHLISREFLPKNIRHFKFYLGHENEIQNIRGLIQLLDNTRPLNNNGQPIQHQQINQAMIFDLFQQNIQNQQQLALIPQLPQTQHIQQLQQQIQQPPQIQQQILQQPPHQPQILQQIQQQPPLQPQLTPLQEILQHQLQHQHVQPPPLHQLLQQPIHLPQTITLTPQQQLELMLQLFENQQDDNEEDEEEEEEENQVESGQQNGQQNHLQEMINQQNQDTLNNLMVANDFITNLDISVHFQFLPGNLPPRLTKLYLDDYNFKLVAGMFPETLLKIVLPYFNQPLEPGIFPINLKSLQLSSFNRLLRAGVLPAKLENITFSRFNGVIEPNALPNSLSRIVFPNYKRTLEPNIFPNSLTYLDLSNYKAGLQRGILLQGLKLLLINDSIGPLEPQTLPQSLTKLSIKDFVGNLDVQFIPPLVNQIHISHLEALKPISTWPSTIKHLEINSQTKYDQPQSHRNRKIPEGFLPNSLQTLEMMGCYLLPKQFPTLLNTLIFHSLDRQFKEKEIPNQVKKLVVKRSNQNHIGFIPKDIEVLEIDKKELVRGQISKRLKKLKRFYVANQLEAFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXI5</accession>
    <name>A0A8J4UXI5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tetratricopeptide-like helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007696</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000425">
        <property type="protein sequence ID" value="KAF2070980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXI5"/>
    <dbReference type="OrthoDB" id="2017782at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="346"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="598" mass="69543" checksum="C53E6AF501200EC8" modified="2022-05-25" version="1">MNVLKFQKSLKYFNHLRQCRTLSKYSISSNSNQNILFQTPSSPSSIDNSFTCRSISTTSPLFKEIQQQQQQLHHQNVHEESSPKNEIDKKYQHYISSALEKFKVNSIDAALGYLDKAILLCDNKVQAYLLRGDINFEIQSNHTVDPINDYLKALELVSSTNNVIDESIATAQEQQNAIMSSIVDYYLLKRNIEKAKHYSMKLIEKNPTIDLYKYEYFNLLCYAKKIPEAAEIAKGLYQTKSDYGFLALGIVSRYQNYIDHSESLLRQITKMKQPVEIQTSNPLYNNKYVISKKGGYQRISQKTVEVENQVLFMANSILGDIHTNNKKEYEKGLEFYRNALALSKMGYVYSKMAKALLKMNDYQAALESINSALEFEEYYSSGTATLHDIELRGEILYHLGKYVESVSDYDHYLEHSFTMYPYHVYEIVKRGPSLEKVIISLAHLSKSNKVNLTPTQYQGPAGTIDLKKEYDKLNSLYLSDQHHPITDIKQLAAISEKYCLFFGKYRDLIQQGRVLVDEKDQQDINTRYLFFYNINSLILDSCYLDYQLRDKNNQLTKDIIDNILKDDKYVESNQPFERQEIHKNLIKVFSDFIQQHQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXM4</accession>
    <name>A0A8J4UXM4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000032</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000001">
        <property type="protein sequence ID" value="KAF2078661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXM4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2078661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="261" mass="30227" checksum="671ABC6A6BBC0D0F" modified="2022-05-25" version="1">MQNIPLPKDHICSTTGKCVYLQNFFRNFREEFKIALPIDKDIIYTPETIETSKLYHICFSKYIELIVGEIIEKGLQMNFTAKQEEAILESTKDRPELISFKATLKELKIKEAQEKAERARLLEIERIAALPPLVYVFKQTEFFPDLAKHLKNAFPQYNFEDKDITNFSLDPNPKYQILYAISSSGVRDPIVSAEKMAVIANHFPQNVIITNCWFVNGQVQISSSNNLNIYYEGRGFQPNQQNIKKLGDEFEKNFKLIESTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXP3</accession>
    <name>A0A8J4UXP3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ELYS-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007492</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000403">
        <property type="protein sequence ID" value="KAF2071190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="20729at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052620">
        <property type="entry name" value="ELYS/MEL-28_NucAsmblyFactor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21583">
        <property type="entry name" value="ELYS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21583:SF8">
        <property type="entry name" value="PROTEIN ELYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1181"/>
            <end position="2039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1184"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1237"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1358"/>
            <end position="1389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1399"/>
            <end position="1408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1412"/>
            <end position="1433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1475"/>
            <end position="1493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1497"/>
            <end position="1506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1513"/>
            <end position="1522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1525"/>
            <end position="1557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1568"/>
            <end position="1593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1605"/>
            <end position="1621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1625"/>
            <end position="1638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1662"/>
            <end position="1676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1677"/>
            <end position="1686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1695"/>
            <end position="1704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1735"/>
            <end position="1754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1762"/>
            <end position="1779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1780"/>
            <end position="1791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1802"/>
            <end position="1968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="2003"/>
            <end position="2013"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2014"/>
            <end position="2039"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2039" mass="235034" checksum="025EE8BD8F8F2045" modified="2022-05-25" version="1">MKKSSSTSMTTQQLFPIESPIESIGLYPTPPTILEPTSVSISSTITSDGRYSWYFKDNEIFLYELASGKRITTWCFVDEGRDLPVTAVGEIYLNNILYIVASIQYHENNNHLKVLDIESGDIVRTLGLNEFTVSFIQQLKKYSDNSSNDNSNNNDCIKQMKCPIALGSKNSCSVIIVDFYFDTFDKSIELSSIIYSLVLQDYNVYNGDGFYQNNNNKSTLIPFFILGDSNGNNRFDKPFEFLCNVNELEGSVVKTMESNSLKKVKSHKSAHVSCICYIECIQCIAVGYSMGGYQLFSSNDFSLCFSSAYREVVSSAVTHFLYQELEDDEDSTVGHMWVGRGTSVNPFIQPAATENKVSLLLYRLDFGKSKHIDDLRLVYSQNVDIAKVSGTHSLGRVFKCNYLHQGSESVLKSKACFTYELLDNAGQPKLCIKLFDLLLFERHGCTSPALESEPWLVEINIPQQYSLRKHFYACWLDANSISKRLYQDDNNDDYLFGGATSPPRVQPFAVNHSSFNIKLLSKGSIDTFYYLNPQEVLIDMMREKGAMVFVNPALTYQKFVDAKVFPTFFTGQDVVEQQRNNLLIFALNNNMFGLIIDYVCGGSRHHSNNANPKLILELVWNYYENIKTHFNINDIFSTVAQNARGNIGESFKRQLENVHVRILDIIGIFKSLVDRYLAENGASAAVPESLQSKLDNAIHTAQYLGVIKWAANNGLFSTITLSSLSQLNVYIVQKRKFFKELNNELLLIDLLYKDCEIYESHTYPPQNIQQLQSFFNLFKSPNHFSKKLSILYYFLIDQTLLEEDNRVEGGYNFVDSFLHIFSINLSEKYFIEGIWELDTHFLYESSDAILKKSFNYLNSTSKTKKYLYSILSRFFKVGHHSNAQLLMKGFGFQPESIDQVSLALEILLANHAVLESILYQRNFRCHHPELDLQSIEKLLFFIFDFTFKNKNLDRIMEFALDEVEEQVFLHYLFKINASNLISLYYIKRGRIPEAIRAHKTYQAGRIGTDAFEGYDTMNNLIMNFEQSLNQSQKFFGNNVNGKEVPLEDIPDLPRSISKSKSASTPNTFIYQPRIQTTPIPRFYNDNIQFSSILSNQPIENLDLLAKKQQEKQMEQVRLLQQIQDIERQKEEEQKRLEIERQNAIIAKEQEYLEQRKKAEEELARQQELIKKQQEIIRKQEEKIASTPSPFSSPSSLKSALKPHGSTSKKKTNVRFKESLNVEYAIPSKEQERWKYYDDDDYDEDEEAEEEEEEEDDDDEMADFDDDNDDEDDDNDQQQQEEDDDTDEIQDDDDDDEMVLNDEEMMPLPDDSDDDQNKSSDDDMAQDDQQDNQFLSQAAPNPNPFALFSVQPSFDQDFPMMPDMPMMPNMSSTTMPNMFQPPFSQSTQQPRPSPFVANDPTLSSRYQQSQDDKIEEDEEYEEDEYYEEDEEDEYQNNLSAEQRARFQSGGSEDQPLEIISSDEEAQQKPSQTTDQQVEDSDDDDVEEYESEESDREQQQQPKQTKAQLRFIQEPDSDDSDVEDQSNVKNSGKNSPSSSSSSSSSSLTSSSSSVSPTQSPIRENIKKDDSSDDDNGNDDNDQYEDNDDIEFDQDDFQPSQEVKEKTTTSSDPPAATDSSTNVVDANDLSDTDVDEEDDQQDSQVVFEPLTQKDQQQQQEEESSTIDKDESIVSEKGDESMVEEEEEKENESIISEKEQEESMVDDDEKVKEPSIIEEEEKVNESIISEIEQDESMVDDEKAQDKEQESIVSEKDDSIISIQEDSMVEEEEEEQEQDQEEEKDQQPKDKDHIDDEQMNSSQMVEEPMEEPIDEDQDVLFDQDDMNEKEEEQEEQEEQEEEEEMDVHEVEEEEEVEEEVEEELEVEAEEEAEEEAEEEAEEEVEEVEEVEEEAEVTEEVEEVEEIEEVEDEVINEEEPIQEEQEEEEEEQVLEEEEEEEEQVIQEEEEQEELVQEEEEEIIEEEAEEIESEEESPRKQKIIPITTRPTRRANLLAKETVQDMSPIKRLSKRENKKKSSSSSLSSTKKPSSSLSKSSSRSSKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXT9</accession>
    <name>A0A8J4UXT9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Survival Motor Neuron Gemin2-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007769</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000433">
        <property type="protein sequence ID" value="KAF2070903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXT9"/>
    <dbReference type="OrthoDB" id="21590at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22851">
        <property type="entry name" value="SMN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049481">
        <property type="entry name" value="SMN_G2-BD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20636">
        <property type="entry name" value="SMN_G2-BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Survival Motor Neuron Gemin2-binding" evidence="2">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="95"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="159"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="239"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="329" mass="38816" checksum="404A32CA04489B14" modified="2022-05-25" version="1">MNFSNNNNNNSNKKKKSTSTTVETNSMMISSSKKSMNQDTNNDVWDDSSLIEAYDEAINAYINAHDENKSSNSKRKQDQKSSQKNIGSSSPMKQNNSTTSTTTTTTTNPTPKERKKKVLVEGQEHNNYPRNGYYSQQSPPTQYHQFHNHQQHYIPPPHYQQQPPHHYNPYNNSQYHHPPPPPYQPPNHFNIPYQNNFNPLPIPPTIPNDDNNLTDLLLSWYYSGFYTGVFQERKRIREQQQQQQQQQQQQQQQHSYQHYYQQYPSYNNNHNNDEEEYEEEEEEEQEEYISGDNNQHYKQETRMNASKPKTATSPLKVYKKNTQSSKQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UY51</accession>
    <name>A0A8J4UY51_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007235</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000374">
        <property type="protein sequence ID" value="KAF2071443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UY51"/>
    <dbReference type="OrthoDB" id="17066at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15827">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 2-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15827:SF2">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 2-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="192" mass="22882" checksum="76A3CFE221B1DFF0" modified="2022-05-25" version="1">MMEIIKSCFKSYSELSQQWKTFRDKGTKLTNSLVNKHLTLSYIDLNDYSDSMKNNIDLQFKFKQQLFKEIEIEYEKLCSVQFELKSIYDKLYQNLTKAKPTFEKLLLSQSAATTTTNLSYDQNLLLKQYQNYEEIVRMYKLSLDNKQTIIENLMQFDDRDQLVVLVSTWNNDTHINYQKIDLIQEIFINSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYD2</accession>
    <name>A0A8J4UYD2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ABC transporter D family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_006157</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000271">
        <property type="protein sequence ID" value="KAF2072540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYD2"/>
    <dbReference type="OrthoDB" id="422637at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015910">
        <property type="term" value="P:long-chain fatty acid import into peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042760">
        <property type="term" value="P:very long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03223">
        <property type="entry name" value="ABCD_peroxisomal_ALDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000636">
        <property type="entry name" value="ATP-binding cassette sub-family D member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF67">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY D MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06472">
        <property type="entry name" value="ABC_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="353"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="220"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="510"/>
            <end position="729"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2072540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="733" mass="82813" checksum="ACDB720974346808" modified="2022-05-25" version="1">MDTNLVSSLIPDNRAFKRASSKLAALFSATQHRLPTTIGKKVLLLAAIGGGAFSLVRFIKTKKNNKQSALVLDIKDKDKDQPKKKNVTRVDAVFFRRLAKIVKICIPSMRSKEFLSLLYLTALLFARTMLSVSIAEIAGKNAQNLVARKWKEMGEGVLKFALVSVPASFVNSSLKYETDMLALRFRKRLSEYVHKEYLEGVNFYKASHLGGSDKIDNADQRVTSDIEQFCNSMSNLYTTLFKPVLDLVLFTRKLVVVMGWGSPLLMFGYFIVSGFLKKLIMPPFGRLTAKQSELEGNYRTVHQRLITNAEEIAFYDGSRKERQIINLSFGDIYNHTSYVSYLKCLVGIFDGFLVKYCASIVGYGCMVLPIYFGIRGSSGKDSTELTKDYIRNTQLMVALSQAIGQLVLLGNKITNMAGYTSRVSELLEMIKSIKERGTSQFTIVHEDEIPLSSANSPMISDKYDAAVDMSAWLQEWRRRSDELRAVKRSLYNNTVRQVSGGGTFVEGDYIKFEDVSIVSPEGKLLVSDLSFQVNPNSNVMITGPNGSGKSSLFRILGELWPLHCGTVIKPRKEDILFVPQKPYLVLGTLRDQIIYPHNAEDMKKFGVTDDDLQHLLTTVDPNGTILRQWSWDNRKDWNSAFSGGQKQRIAMARLFYHRPQFAILDECTSAVSDEVEDKIYNTCKVLGITLFTVSHRPQLRAHHDYQLLFDGRGKWEWTKINAAEDMKKPLSFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZ17</accession>
    <name>A0A8J4UZ17_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="3">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_006338</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000288">
        <property type="protein sequence ID" value="KAF2072343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZ17"/>
    <dbReference type="OrthoDB" id="1902587at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042282">
        <property type="entry name" value="FKBP6/shu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46674">
        <property type="entry name" value="INACTIVE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46674:SF1">
        <property type="entry name" value="INACTIVE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0697">Rotamase</keyword>
    <keyword evidence="2 4" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="6">
        <location>
            <begin position="51"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="266"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2072343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="314" mass="35121" checksum="D4D7892A7C28E586" modified="2022-05-25" version="1">MSNKSLTSSGERLIGSNSPPLKKPGVQLDSDGCLIKRVLKEGYSDTTPPPKSIVSILYEGYLSNNNIFDSNVQQQNRSPFTFQLGTNSAIEAIEIAVATMKLGEEAEIVTTQRFGFGKLGLPPFIPPNTTIIYKVQLLSIQLFEDIDNNTGISFNNSTSIDVFDIWIKKALKEKELGNQYFNSNNYKKAIRHYIKSIWLLNDPRLGGVSFSGDKEQNDSNSSNSKQLKDLLIVLYLNLASCNIKLQDGNRAIANCEKVAEMGGNTAKFYFKMGQAYSLNKQYESAKRCLVQAIRLEPNDKILRDELEKIKLNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZ89</accession>
    <name>A0A8J4UZ89_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1348 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004439</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000156">
        <property type="protein sequence ID" value="KAF2074265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZ89"/>
    <dbReference type="OrthoDB" id="14527at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009783">
        <property type="entry name" value="DUF1348"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31757">
        <property type="entry name" value="SLL0781 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31757:SF0">
        <property type="entry name" value="SLL0781 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07080">
        <property type="entry name" value="DUF1348"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="159" mass="19246" checksum="8E41F714AA7E0CE0" modified="2022-05-25" version="1">MSQEEEKRYPLPPFTLESAKQKVRLAEDGWNSRDAEKVTTAYSLNTNWRNRAEFIQGRDNIKQFLIRKWNKELEYRLIKELWSFNDNRIAVRYAYEWHDDSGQWFRSYGNENWEFEQNGLMLNRYSSINDLPIKECDRKFHWPIGRRPDDHPGLTDLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V014</accession>
    <name>A0A8J4V014_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004892</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000182">
        <property type="protein sequence ID" value="KAF2073798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V014"/>
    <dbReference type="OrthoDB" id="429467at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000636">
        <property type="entry name" value="calmodulin-like protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050403">
        <property type="entry name" value="Myosin_RLC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23049">
        <property type="entry name" value="MYOSIN REGULATORY LIGHT CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="7"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="69"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="139" mass="15566" checksum="48415B841286FCD1" modified="2022-05-25" version="1">MDLLTDEQKNEFQTCFQQFDKDLDGRLNPKESVMALKSLGVNLPESEVSAGTDFNSFLQVVVRKLQITDPADELQRAFNCFDLEGTGFVAVQYIRQVLTSMGDILNGQEIDQFIRDCDTDNDGYISSAEATKIILSKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V083</accession>
    <name>A0A8J4V083_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="10">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_003377</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="10"/>
    <dbReference type="EMBL" id="AJWJ01000105">
        <property type="protein sequence ID" value="KAF2075300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V083"/>
    <dbReference type="OrthoDB" id="10261556at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17920">
        <property type="entry name" value="DEXHc_RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.80">
        <property type="entry name" value="HRDC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004589">
        <property type="entry name" value="DNA_helicase_ATP-dep_RecQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002121">
        <property type="entry name" value="HRDC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044876">
        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032284">
        <property type="entry name" value="RecQ_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018982">
        <property type="entry name" value="RQC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00614">
        <property type="entry name" value="recQ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF120">
        <property type="entry name" value="BIFUNCTIONAL 3'-5' EXONUCLEASE_ATP-DEPENDENT HELICASE WRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00570">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16124">
        <property type="entry name" value="RecQ_Zn_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09382">
        <property type="entry name" value="RQC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00341">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00956">
        <property type="entry name" value="RQC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50967">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="81"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="275"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="HRDC" evidence="12">
        <location>
            <begin position="648"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="734"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="737"/>
            <end position="771"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2075300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="891" mass="101625" checksum="4A7095973BF5CC45" modified="2022-05-25" version="1">MFSNKNNSNHSNNNNNSNSKKLTDQEIDIRIEKVLKKYFGLNSFRNHQREIIKYCIDKYNEINNSGINRYNQLATNDNDDDPSHINAGDTFVSMATGSGKSLTYQFPSLVLKKTTIVISPLKALISDQTLKVQSLGIKAVQLSAGEIPLDVYKNLLKGYYRIIYVTPEKIMNSMDIIEKMVMNNVICLFAIDECHCLSEWGHDFRTSYRQLSILKSLYPFIPIMALTATSTKEIEDDVIQVLQLSNPLTFRSTKDRPNIFYKCMLKENENKDFQMILNIIKDVQKETNESGASTIIYCRTIDQTVILSNYLIQKGIASSCYHSQLTDKKRETILREFLFNSLQVIVATIAFGMGIDKPDIRLIIHYGPAASIEQFYQESGRCGRDGLPSLSLVLFSQQDFVKGHFRITSHQENMLTAQQKATKLNQMKFFLLNKTTCRRKLLLASLAEEYPLENCGSCDFCTEINDTNRLDLGNETLLMIRCILESHQRFGLSTVIKILRGSKGESSTKHSKNQYFGKGTSFSEKFWKELGTQLKMEGYLEESVQGLYTITKIAKKGWTLLRSATISELKTTKIPIVLLQSSTIFLKEFKLSYYSKSKLNNNNSNNNQDNSNGNSSQNQNSFFQLKLVSNSINNNNSNNSLELDYSLDDTTLELFKILIEYRKNLSQILEIPPFLLFSEKTLRSIAKIKPATLDSLGKIEGLESKKIKDHGISILLKVKEFLSSKNLNTFDQFPIKNNNNNINNNNNNSSNSNSNNTNNNSNNSNSNRVNSPHTTISNVIKTFNTNNQSNNKPNINIPTDDNIVKRKYDDFNSNTLNENNENQTRKALKLTINNNDNNNNNTKIEPSDDTHSFQLLYDKKVEIHLESSTFIANHIKSIRDQAYRNKLKQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0J9</accession>
    <name>A0A8J4V0J9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNAHis guanylyltransferase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009363</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000689">
        <property type="protein sequence ID" value="KAF2069317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0J9"/>
    <dbReference type="OrthoDB" id="20262at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008193">
        <property type="term" value="F:tRNA guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024956">
        <property type="entry name" value="tRNAHis_GuaTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007537">
        <property type="entry name" value="tRNAHis_GuaTrfase_Thg1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038469">
        <property type="entry name" value="tRNAHis_GuaTrfase_Thg1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12729">
        <property type="entry name" value="TRNA(HIS) GUANYLYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12729:SF5">
        <property type="entry name" value="TRNAHIS GUANYLYLTRANSFERASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04446">
        <property type="entry name" value="Thg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNAHis guanylyltransferase catalytic" evidence="1">
        <location>
            <begin position="25"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="281" mass="32819" checksum="B3A432B175F54D7F" modified="2022-05-25" version="1">MFKINTPKIIQQCKRYYTNGIQNKFNEIEKSFDFVLPPYQATIIRLDGKSFGKMTDNIKLNKPHDNRFHEAMLDASMALCNHFIGTQLVYSFSDEINLLLYSNYPSKQFLSNRIQKLVSISSSIASVHFSNKLESLLNNDKQSQQQQQQQIYSFFDSRVFVLPFEDVNEYFANRQKRCYANCLNSIISSNRNGNGKGNENVLGRKLKFQQEYIEKDLLISLDQYPDHFQKGFLITKSINENDDSVKWICSTSTQSTNNNNNDNSKLTWFHKDPNYIYNLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0Q5</accession>
    <name>A0A8J4V0Q5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002609</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000073">
        <property type="protein sequence ID" value="KAF2076095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0Q5"/>
    <dbReference type="OrthoDB" id="24030at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35885:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35885">
        <property type="entry name" value="CARBOHYDRATE BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035283013" evidence="1">
        <location>
            <begin position="21"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="309" mass="34992" checksum="B3CDB8B9C0EB26ED" modified="2022-05-25" version="1">MKLIITLLVLFLISIGYTRADTYSVVIWNNGTSVNVGLADVTSGNQAQLKLTLQDFYYQNPVIQSSTYNYATKLLSFYTFQISNRIPTLFVVDCNSWTVVSKTTYNSVFLYGGLASTNTPDNNVFTTYTDNSYIYVARLDSSQNIVKFDAVYGSYRGSVYVPTIDSYYVAFTNKTGLYVRIYNSNYQLSSEAAFGFSNNRYPVNDFPLNLVYDAKNNGVLAQVSMTDSRNNLYYALAYLDWNNNTFDVTNMYGNLKDRFTTTVPDSNNLDYAYTFAYLNFGATNLYIFNTATNTFVSSRPYNTLVLSAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0U6</accession>
    <name>A0A8J4V0U6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_003818</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000125">
        <property type="protein sequence ID" value="KAF2074863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0U6"/>
    <dbReference type="OrthoDB" id="24183at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057709">
        <property type="entry name" value="DUF7949"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF29">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25820">
        <property type="entry name" value="DUF7949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035304833" description="EGF-like domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="5">
        <location>
            <begin position="426"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="4">
        <location>
            <begin position="660"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="3">
        <location>
            <begin position="784"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7949" evidence="6">
        <location>
            <begin position="1037"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1005"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1010"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1023"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2074863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1137" mass="127563" checksum="8707833C0B9A5140" modified="2022-05-25" version="1" fragment="single">MVHPYKVLLLFFFLGVIHSFTVTKQTNEILNLLGPINGNCSRESSLIISDLVPHELLSYSVQPNFGYSIFVTYENASAKVVSFQTKLPFGPNPIAVSLDIASNVSQTVTLNMLYECTPPPIPIVKNLGFFYPMEMGPVYYYMLKVENTNAGREKIFYAQTNHSNWAAIPSFYSMDIITIRLSNSFASPPVEVSYVEVMVRFSYSNNYDHYVNVEIPLLAGPLQISNLVAQVFNYRSYYYPFIFQATQNLPFKRKLYVIDDPSQTRKAVASPVYGTLLNATYQFVMPVVGSTFNTTLWEIRQLDYGLLANERIFTTTPTYTNYWPLGPSGFEVVLYPEGSGSLAIVSSQRSNITRVESAFFFSYQLDSLNFYYSRDLMDGRYGMTGGNYSQFTFESSIWVSIFDNRPSFYLTVQGPSLPITNPNPYTLDNIPPFLTDLKLITIPGFNRYIIARITVTDDRSGFRYLSGVSNNNLNGNMLISSKDIFRGTGNDGVYETVIENPLFSLNIYSVIDYSSNSYSYGPEFQTTEQDRRLVFSNPPNSYVQQMNPRIFSNVTLASWSHNDIDVSNSSFTTIFRFNATNPDKDLSPFLDLLDFGYQENTRFFGQWNDTIKQYEVPVIIPLRAMSGVVTFKITYFKAFDSGFLNNIFPLSTLRIHSDDADMFGPTLEVTRLIDITDGALTIGWSFKVSDRLNGFHSGHFSVVSERHQNIYEFVFDPAKETNNFTLHFPHENCLSQIFYISNVYLRDNGGYISDYNRAFIDKLDDNSISIDLTCPPLPEDLAPPTLSFMDFQPNAIDVGSKERVVTFTFTIQDTGNGLLLEKLPFVYLSSNNEIAKEKATLLDGSTPFKADYSCSFTVPYGFGYPGYSLVSIYGSVDVNGNYGGFSSQTIKGAGFSYILDTSTFSFDADPLIESNAYFTNNGGKLMIMGKSFGTDNSSVVQIDYNDTSGYSQTSTPSFSTPSTLIINDVKPTNTSFKIQIIKSKNALIFYSNEFWVHPFIQPGFPPQRESSSSSSSSVSSSSTTNSEEIPTQAPNQCLNNCGGPSQGYCSATGCICHSPWMGIDCKSKVIIIPTPSINNTTPSTNVTVPTDNSEKSTLTGLISIVELRELDKNYQLVYKYPFTQWIWSDISTDNEPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1T6</accession>
    <name>A0A8J4V1T6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dolichyl-P-Man:Man5GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase</fullName>
            <ecNumber evidence="3">2.4.1.258</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_001248</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29527"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="Rhea" id="RHEA-COMP:19516"/>
            <dbReference type="Rhea" id="RHEA-COMP:19517"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:132515"/>
            <dbReference type="ChEBI" id="CHEBI:132516"/>
            <dbReference type="EC" id="2.4.1.258"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:29528"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.258" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000028">
        <property type="protein sequence ID" value="KAF2077475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1T6"/>
    <dbReference type="OrthoDB" id="20028at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052925">
        <property type="term" value="F:dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007873">
        <property type="entry name" value="Glycosyltransferase_ALG3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12646:SF0">
        <property type="entry name" value="DOL-P-MAN:MAN(5)GLCNAC(2)-PP-DOL ALPHA-1,3-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12646">
        <property type="entry name" value="NOT56 - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05208">
        <property type="entry name" value="ALG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="102"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="186"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="303"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="414"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2077475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="449" mass="51688" checksum="DD79A8F075F00B24" modified="2022-05-25" version="1">MTNITNQSFTKPGNRVKNDNVGSGSIGFLYKSSKSFVSLISKSDNFIYIALLLLAFEFILNKLIIDNVSYTEIDWSTYMQQIELFVNGERDYGKLEGSTGPVVYPAGFIYVYSILYQLTNQGKDILRAQYIFAGLYILVTAIVFAIYKRSVYESQAHPALFKQIPSFLIVFLCVSKRIHSIFALRLFNDTISMLLFYVSLYLFIRHRWSFGCLFFSLAVSVKMNLLLFAPSLLLLLLMTFGILRTIPKLMICAAVQVLVAVPFLQENVANYLIRSFEFSRQFMYKWTVNWRFVPEDIFLSKPWALGLLSLHLAFLCLFIFTKWCKKDGGIVKCIQRGNANKNPALLSTNFILTVMFTGNLIGITFSRSLHYQFYLWYFHSLPYLLWISNLPNPIKLIVFVMTEFSWNVYPSSNFSSFILFFFNLLLIVSLYKSNVPSSTTKIVSNKKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1V0</accession>
    <name>A0A8J4V1V0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">S1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_007864</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EIF1AD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000443">
        <property type="protein sequence ID" value="KAF2070817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1V0"/>
    <dbReference type="OrthoDB" id="1738325at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039294">
        <property type="entry name" value="EIF1AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001253">
        <property type="entry name" value="TIF_eIF-1A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21641:SF0">
        <property type="entry name" value="RNA-BINDING PROTEIN EIF1AD-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21641">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00652">
        <property type="entry name" value="eIF1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1-like" evidence="4">
        <location>
            <begin position="23"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="121"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="151"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2070817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="165" mass="18762" checksum="23E013326CE87697" modified="2022-05-25" version="1">MSHSRKHVTDKTLNSDITVGEGHTVVKVVSIRGGNICEVQKPNGEQTLTIIPAKFNKILWIKNGSYAIIDKEDDDPKSKIRSSIVNILSKDNIKELKKSNQWPIEFEDQVDTNKRTQIVKQKVVKNDDDSDDDSLSDLMENPNHKKHAFIDDTDSDEDDDEEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V450</accession>
    <name>A0A8J4V450_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Inositol-1-monophosphatase</fullName>
            <ecNumber evidence="12">3.1.3.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_005468</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>a myo-inositol phosphate + H2O = myo-inositol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24056"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:84139"/>
            <dbReference type="EC" id="3.1.3.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>alpha-D-galactose 1-phosphate + H2O = D-galactose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:29315"/>
            <dbReference type="ChEBI" id="CHEBI:4139"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58336"/>
            <dbReference type="EC" id="3.1.3.94"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 11 12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 12">Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 12">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.25" evidence="12"/>
    <dbReference type="EMBL" id="AJWJ01000219">
        <property type="protein sequence ID" value="KAF2073217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V450"/>
    <dbReference type="OrthoDB" id="10254945at2759"/>
    <dbReference type="UniPathway" id="UPA00823">
        <property type="reaction ID" value="UER00788"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008934">
        <property type="term" value="F:inositol monophosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01639">
        <property type="entry name" value="IMPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000013">
        <property type="entry name" value="Inositol-1-monophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000002">
        <property type="entry name" value="Inositol-1-monophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033942">
        <property type="entry name" value="IMPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854">
        <property type="entry name" value="INOSITOL MONOPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854:SF4">
        <property type="entry name" value="INOSITOL-1-MONOPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 11 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00035990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2073217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="272" mass="29731" checksum="4B44BBF757A3363A" modified="2022-05-25" version="1">MMEISYADYLQSAIDVAKDIGPTILKNYNSRERKIEYKGAIDLVTDTDKAVEEHIIKTLTGKYPHTKILGEESTKDGIYNWGNEPTWIIDPIDGTTNFVHRFPLFCVSIALSINKEIVAAVVFCPVLNELFTATKGGGAFLNGEQIFVSSVDTLSNAMVATNVGYDRTKAGIDHMLGNLDNILNQNVQAIRFSGTAAWEMASVSCGRLDCFYEWGIHPWDIAASTLLIREAGGVVVDPSGSDVNMEGRRVLCGGKPIVDLLSKLLLPKPSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4Q9</accession>
    <name>A0A8J4V4Q9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TraB family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004896</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000182">
        <property type="protein sequence ID" value="KAF2073802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4Q9"/>
    <dbReference type="OrthoDB" id="48306at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14726">
        <property type="entry name" value="TraB_PrgY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002816">
        <property type="entry name" value="TraB/PrgY/GumN_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046345">
        <property type="entry name" value="TraB_PrgY-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21530">
        <property type="entry name" value="PHEROMONE SHUTDOWN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21530:SF16">
        <property type="entry name" value="TRAB FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01963">
        <property type="entry name" value="TraB_PrgY_gumN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="527"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="486"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="501"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="554" mass="63812" checksum="47EDE088B13997D2" modified="2022-05-25" version="1">MSELQQQNINDIINENNSISKQQDNTFLIVDSNQPPTPLPNQQQLNGNNSDDINFELPSSAKVIHSKITGSTIILVGSIHIHQSSSDEVSEVIRKWRPDTVFIELCQSRVGVVMDQQPKKVEIHKKDDDIINIRQNRFKRFGKVFQKNNDDFEQEEEDDYRDNTFLEDTTTNTNPFDDIDVNDMTQEDNEIYTSEEQEEEEEEEEEEDGFDENDTFYNEEEDEIYFYGDDENESDSESSYLKNNSCVSPVISNPHYFDRQNNPDTEADDEKSNPPSQYHSLSSTPYTTTPPTPVGEEVTDNVSFQDMINIIKANGLSGILHVLMGELIRKAGKKSNVGPGAEFLTAFVEAKKIGARVVLGDRLIEITLQRVWNSLTRWEKMKFVFYLLWASLSDVTKEDIDAMRNSSEELINQLLNEFREKFPSVIQTIVTERDQYMAANLRVCQGKKIVAVVGKGHIPGILREWENYNINLKELESVYVNRNRNSNNNNIINDGNHKSKSNTNENSDNNNNNSKDSYFQDMDDHSWITKWASFLLIPMVGISLYFFNKKFNFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5J1</accession>
    <name>A0A8J4V5J1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tyrosine specific protein phosphatases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000139">
        <property type="protein sequence ID" value="KAF2074597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5J1"/>
    <dbReference type="OrthoDB" id="193277at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38745">
        <property type="entry name" value="PHOSPHATASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38745:SF2">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035298963" description="Tyrosine specific protein phosphatases domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="253" mass="29696" checksum="41B92BE50DB56856" modified="2022-05-25" version="1">MIFDKRGFIFCILFLSFAFIYVNAGDLDYKKVHYIQSTPPLANGNVNYIFRGNEPRENVNGTTIFAYDDLVSFIKQNGQENNITIPDQFYLIDIKLITGPLPSELPDIELEQNFFAQNSNLGEFHTNNTFGDLLDPQWFDEEEQISMAKTLPNWQKDDLQYRMDLYHNILFTERDLPVVLYIHCECGCDRTGEIMASWVMRFQGWNFAQAMEWDYSIAGRKINFANQWATQWYCLYLQYAENRSIDCTPPANW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5K3</accession>
    <name>A0A8J4V5K3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000136">
        <property type="protein sequence ID" value="KAF2074657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5K3"/>
    <dbReference type="OrthoDB" id="20930at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="105" mass="11728" checksum="FE2BCBAA3C40D4A5" modified="2022-05-25" version="1">MYIFILIAFIVGYLLSHFNAISFVYSTIVDITIITVANKKIIFAVFSIQCLLYFLIYLPLSKVANQLINDEKASIKLYNAALLPLQTQLQSPSPLTIATPTLSNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V675</accession>
    <name>A0A8J4V675_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003183</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000096">
        <property type="protein sequence ID" value="KAF2075542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V675"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="506" mass="58030" checksum="D19763148624C017" modified="2022-05-25" version="1">MTDISFYLIWRNQYIRKLIRNLLCQDLVINVNDKYLNEYHRHLSLFTLQDKIDYKICVRFNSTFNNYVNNKYKSLVNEIALEVDIDGVADYNLNLLEHGLTTLTLGIKNMTTASGQLPESLRRLDINIYERECESPPLLQHILSNLPCNLEMLCIPYQYEMTRECVLPQSLCELYYCSRYQSLGKFVAPPAKVYKGCALELDSMDAFRWMVENPWVREISYNRDLDFKDKVLPPHISAIKIEQGINLDQSVLPKMLESLFIYEGIHFSKLAYLRVLDLSHDYPVKLEKGIFPQSLETLSMDYDLPIEIEVFSPNLHTLHLRTFNQPLCAGSLPDSITILYLSFFNHPLQLCSLPSRLKVLSMRNYKQASISSLPSSLTSLCISSFAGSFQPSCAPLNRLKKLLIRSIDPTLSTTLLTNLKRIKISVSQLQSDTSFLNTSLESLHLTCHQKCTLSANSLPPTLKYLTLTNTDIQSNNVVPPSCTYLKSCNQDINPQFIPQCVTYIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6A8</accession>
    <name>A0A8J4V6A8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small-subunit processome Utp12 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_003070</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UTP5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000091">
        <property type="protein sequence ID" value="KAF2075647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6A8"/>
    <dbReference type="OrthoDB" id="30195at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007148">
        <property type="entry name" value="SSU_processome_Utp12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052414">
        <property type="entry name" value="U3_snoRNA-assoc_WDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44267">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44267:SF1">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04003">
        <property type="entry name" value="Utp12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="89"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="173"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Small-subunit processome Utp12" evidence="6">
        <location>
            <begin position="408"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="505"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="522"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="570" mass="64329" checksum="9FBA528138B28EE2" modified="2022-05-25" version="1">MSHSLFDNDGALFARITKDNRVQVFNSLNAKLVQTFSEKDQSNNQNTCIAWSSLKSTDKNINNKLLACGTKKGDIFVYDLIDMKRVAHLRGHNTKVKDLIFSTKVANCLYSADNEGNVIQWDVSNPKQKVKRFRADSKSLSWMAINKESTILATCDHDVKLWDLSSLEMLTKFSAHQSEIKDICFSSDSQYIFTTSGDRYICVWSASPSKEQQESSKPLLTLTSESQIRSINTYHINKQHIITALSDSNQISVWSFQDSKLSSAKTTPLSKIILPTNPTLNLTIFSTILIDNTQIMVSYGQITSTKFKKITFYENNSFIENIRIEDTFEDKSNSTTTANKSLEPALKKKKEVLSTQGSVVKVESNNVPLPETVEKSSSKNKIASAGTVIDLITQSLRSNDEVLLYKALRVNFSVVNNTVKLLPPPYAYSLLTRILKDIATDNTKSHVLLPWVNTIIRCHSTYLLSIPNLSKQLSILNAIIEDKFNLHDKLSKLVGKFELVESQSQSNQVTSVDYDPTLVYEEASDDDEEQEQDDDSMGEDEEDDQESEEFASMDEFEDGDEDDEDFEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7J9</accession>
    <name>A0A8J4V7J9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004675</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000169">
        <property type="protein sequence ID" value="KAF2074024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7J9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035249521" evidence="1">
        <location>
            <begin position="22"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="318" mass="35633" checksum="295CB64DD81F5BA7" modified="2022-05-25" version="1">MKAITTFLLLLGLCALGFVNAYPIKGYGNLTPYNIYTFNQQNNQKYAQALIPQPKQVIAISGSSSDVPQSFDIVYSNSKDSITWGSVNYANGKVSDKHVFGVPKTVTLNNVICAQVVVKPDNNTLYYMTTENKYGDDIIYWVASRVESQDDLNHGTTVWPMGYGDNAQGAFDYTTETDYYIVMINKYAQNSYKAFWLDPYTNSTVKVTNLNAPTSPIFKIFAYKETLYLVQPSRSNYDIIEISSVDWSGQTLNKVSSIKVSTQPNQLDIHVYGDYIAIISRGLNQPQAEVVLVELHKFTVLDFLQGLSSGQGSNYFIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VAT4</accession>
    <name>A0A8J4VAT4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MICOS complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001188</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000027">
        <property type="protein sequence ID" value="KAF2077489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VAT4"/>
    <dbReference type="OrthoDB" id="18689at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="16006" checksum="C817CE062D41F0A8" modified="2022-05-25" version="1">MENNNNVEEDGIFRVAARQLIGAKKETDQYLNKINQKEIESVFTDASKAIVSAVDVVSLRDNYNLFQQKMEKVPPVAIVASATILPILLFKKVPSVKDPLLAMIGFGTSMYYCYPEVVSKASKRTLELYKTHVQPLVSPPTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VB86</accession>
    <name>A0A8J4VB86_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Association with the SNF1 complex (ASC) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_000740</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase beta subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000015">
        <property type="protein sequence ID" value="KAF2077939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VB86"/>
    <dbReference type="OrthoDB" id="531008at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.250.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006828">
        <property type="entry name" value="ASC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037256">
        <property type="entry name" value="ASC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050827">
        <property type="entry name" value="CRP1_MDG1_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE , BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343:SF84">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04739">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01010">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160219">
        <property type="entry name" value="AMPKBI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Association with the SNF1 complex (ASC)" evidence="3">
        <location>
            <begin position="249"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="90"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="243"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="11"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="114"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="243"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="347" mass="39378" checksum="1CC9614F516A2CF1" modified="2022-05-25" version="1">MGITSSTEQKSNIQSSNANADQQQQRYIQQQLQQHQQKQLQQQQQQQQQLQQQQQQQQSYNQKINQTTVRPQQQQQPYKPLPIYLTPQQQQYYRQQQQQQQGSKSYGQSMGQSPRSIPSSGSNTGKTPSEFNVLMSPPSSPLVGPMANTAIIDPPIPTVFTWTGGGKEVYISGSFNNWKEKIPLSHSEKDFTLIYNLPPGVHQYKYIVDGKWIHSTEQPVAADIKGNLLNFVEVKSKDPSNDLNTLKLSTTPPGSYSKNIPEEEFQKIPPPSLPPHLRRALLNTQPSTEDPTLLPLPHHVMLNHLYSLPRKDKVTILGVTHRYKTKFVTTVLYKPVNESSLDNLDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJ94</accession>
    <name>A0A8J4PJ94_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010336</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001046">
        <property type="protein sequence ID" value="KAF2068337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJ94"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="433" mass="50964" checksum="CA40E406845702CF" modified="2022-05-25" version="1" fragment="single">NWFGKCLEWGSNELFVSLWNRFKTNDQIRVSETNNSISFCQDSVDKTRHSEYVYDYMACNWRYLNIHIIDFLIGEGCTGVMKQIYLTDDLLRIKYTPKEDFQLIKKFIKLEILEFHQVLVLCIREGNIALFEWLYPLREEYKFEMHELLQELLKNPSTRVLKLLKPEFSQIGWERLLSIKSLKLCFLKQILTSIPHQQNPLPTELLQLLVGNSSYANAKHVLSNYRFESITPILSTLAHRDNPAMVNLIYRYQDTTFINTPTQQDWDSLFKKCIRTNSDRGTTANLEYLINHTDHILSCPIPKDMDSINPNTLSAEAFMLIGMGGYFDPAKHDKFLQQALLHSQSRFPDFFYLIDKDPHYTNDFVQYIVNKSNTGHDDGPILYCLDYLYNNQHKFRDAPSDKLEICREILNQPIPEYLSTHIKSYYLNNKDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJP8</accession>
    <name>A0A8J4PJP8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_010567</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001201">
        <property type="protein sequence ID" value="KAF2068107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJP8"/>
    <dbReference type="OrthoDB" id="76265at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031543">
        <property type="term" value="F:peptidyl-proline dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071456">
        <property type="term" value="P:cellular response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051559">
        <property type="entry name" value="HIF_prolyl_hydroxylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12907">
        <property type="entry name" value="EGL NINE HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12907:SF26">
        <property type="entry name" value="HIF PROLYL HYDROXYLASE, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0847">Vitamin C</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="7">
        <location>
            <begin position="179"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2068107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="290" mass="34112" checksum="78D0B650F92905A5" modified="2022-05-25" version="1">MEDIISKLPSNIKYQILNQLNKDTAATEGQPQPATNQIRNIISQEYLIDLCSDIKSEHLSFFTKDRLHQLEYDGYFVVDDFLNDRDFVERVHNESVKQYQDQLLKPASMNKGADRWHDKQIRGDSILWIHRDPLKNNSNELNRPGVEIYESIPNVNKLLDRLDDIKNELDLVVPSFKVQKSQTQLAVYPSGGKYVKHRDSFFSNDQLNQRRITMIYYSNLNWTKGDGGELRLYTYNKNRKDLNANDKEQDHYIDIDPKADRLLIFLSPFMEHEVLECIKEPRVAITTWIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJV1</accession>
    <name>A0A8J4PJV1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Delta(14)-sterol reductase</fullName>
            <ecNumber evidence="3">1.3.1.70</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">C-14 sterol reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Sterol C14-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">CYY_009731</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="17">Steroid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERG4/ERG24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.70" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000790">
        <property type="protein sequence ID" value="KAF2068952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJV1"/>
    <dbReference type="OrthoDB" id="5326588at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050613">
        <property type="term" value="F:Delta14-sterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000246">
        <property type="term" value="F:Delta24(24-1) sterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1630:FF:000011">
        <property type="entry name" value="Delta(14)-sterol reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001171">
        <property type="entry name" value="ERG24_DHCR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257">
        <property type="entry name" value="DELTA(14)-STEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257:SF31">
        <property type="entry name" value="DELTA(24(24(1)))-STEROL REDUCTASE ERG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01222">
        <property type="entry name" value="ERG4_ERG24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="10" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="13" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="294"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00060577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00069705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAF2068952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="463" mass="54350" checksum="ED65939B428D7A83" modified="2022-05-25" version="1">MSQVRNRNNTEKKVVSDEERLTDQQKKELADLKKVHPANEFGGIIGTFLLTFILPVVVYWIWASLEFNEGYLLRPAELSVEGIKAFFVNLYNIVMEHAYPTKQATIIYFSWFFFQAFLQHFVPGRKVLGSPLPGGKRLEYTLNGWASWWITMIAVPAAAYFGLFKATILYDNYGSMLTVVNIWSFVFTFLLKIQAKIQDDEERMSGHFFYDFWMGFARNPRIGSFDLKLFCEARPGLILWVLMNFSIAAKQYETYGFVSISTLLVCAFHAWYIADYYYHEEAILTTMDIITEKFGYMLVYGDLAWVPFTYCFQCYYLYKHLVAGAPIQITIAYALGVVALKCFGFYLFRWVNSQKHEFRRNPDHLIWGKKPDFILTKRGTKLLCSGFWGICRHLNYTGDIILSWAWCLPCLFDSPAPYFYGIYFTTLDLHRCWRDHNACLEKYGDDWRNYCKRVPYVFIPRIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJZ4</accession>
    <name>A0A8J4PJZ4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_009609</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000756">
        <property type="protein sequence ID" value="KAF2069072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJZ4"/>
    <dbReference type="OrthoDB" id="10009520at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20346">
        <property type="entry name" value="BRcat_RBR_ANKIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22583">
        <property type="entry name" value="Rcat_RBR_ARI7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16773">
        <property type="entry name" value="RING-HC_RBR_TRIAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000022">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000019">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045840">
        <property type="entry name" value="Ariadne"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048962">
        <property type="entry name" value="ARIH1-like_UBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19422">
        <property type="entry name" value="Ariadne"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21235">
        <property type="entry name" value="UBA_ARI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="135"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="139"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2069072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="518" mass="60300" checksum="165C52E9A228D3BF" modified="2022-05-25" version="1">MNTDDEMYEDYDVDDDSAEESGNESIDDMEYDETGTQEFDFDDNGPSRTHSKLTRQKSFEVLDKSDLYSESHKLIKEVMDVLSIPSEACVSTLLRHMKWNKEKLIERYMENSDKLCADAGVPNLKLDKPVDKGIKQFSCLICLEDFSPSQTYALSCDHRYCLPCWKSYLEIKVSEGPECIYTNCPAPKCKVRVHEDAFKAIVSPEVFERFSSFILKSYVDDNPQVKWCPAPGCIYSIRCDRKERKEAVNCKCGFQYCFNCNDYDIGDHMPVPCSQVDKWLQKASDESENVTWMLANTKKCPECRSPIEKNGGCMHMTCKKNAGGCGFEFCWLCRGPWSEHGSTTGGYYNCNKYDKSKAKEEDEKATDAKTELEAYMFYYHRYESHRNAMKIADEQRKNAHLKEQQILSKFEVRSADTKFLMEATEQLLRNRRVLQYSYVYGYYLDKKSQERNLFEYLQEDLEKHTNFLSTLYEQSLDKLEDYQSFIKWKEQVTNYTRITKKFLDNFVEGVSGGLTAVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PK21</accession>
    <name>A0A8J4PK21_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009444</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000710">
        <property type="protein sequence ID" value="KAF2069235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PK21"/>
    <dbReference type="OrthoDB" id="21516at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="53"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="98"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="201"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="319" mass="35136" checksum="81E1012901F219C3" modified="2022-05-25" version="1">MNTENNNNSNNFVGSSSVGGTRVPLIGANKAYNTTSTTSDTPANTPINPPPSNIINDNNNNNNNNSQYTFKTGIMPQQNRPRTLSPLSKDKAAIAVDSNSSNNNTVSSTQLESNNNNIDNNVNSNNNNNNSSSSSNIAFEPSSSQSVINNNNNNNNILINQKKQMFNKLNGSGSIEIFQFDEEYEEELEKEAIQQENNQQTAATAGTTASTSTTTYNDDDDEQQQQTVDGNNRNRSNGLMQETIPISQSLPMGIPSRLTQSLKQNPGASMIVNLDVNNLEKKKYVTPQQSWVEILKEKDEMDSKLEISQSLKRKNKFNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKD0</accession>
    <name>A0A8J4PKD0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_010256</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000999">
        <property type="protein sequence ID" value="KAF2068418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKD0"/>
    <dbReference type="OrthoDB" id="6130531at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035191714" description="EGF-like domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="4">
        <location>
            <begin position="417"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="3">
        <location>
            <begin position="642"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="2">
        <location>
            <begin position="779"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1039" mass="115332" checksum="25C2A39CC18922F1" modified="2022-05-25" version="1" fragment="single">MKYRYFLCIFILFYSFVNISPVNALKAEVLSQQPNDYYANATGYCRVSFYLALVDPQYPVTDIRSYLPHFFIISPFPAFYVNSTTTILFVTLDIRGNVDSSSFPVSFLYYQDSQWTTVPTVDTQGDEYYFKCEGTPQPISQLTLLRPLEAGVMVDGKNSIFRGYVQTDLKRGFRDTTCSSILSACDVGFQGNGVYQATFYLLNTYTGDLTLTNPNNFVITINGSRFEFDNPLSTSLYQSQIVQSIIEGTYSSNSFSGALYAFSSNQNVLPIITEIFVPLIGTVKNFSASMSGSNLQPKFMTSKKLAIGSIIPYSQNVFITNTLSQSKTSGWDLGSLYYMDSSFYVQSSMKYIFGITTTYAAIVDTSYPFGLVSLSEPLPKFKISTMFSPYQSQYSINVGILYWSPAGRVIDGTIGGDTTAPVLEAIIMNPINNTHSVLSLQITDNLSGVGYIGVLDSTTTDNFYLNQQNLVNGTLRDGTFEKILPFQSFANFEIAIFDRAGNKVSFPRNMISAKFGLGISGFQRVDFDITYFKFKQANVDVSTDSVASTLYFNFTLPTDFDRKYTNVQFRPNLTPKKGFESKYYGGYNTATLLYEINFIIPAKTFPSYVPYTLYINDIKVGPYLIVSKFESDSRLLVTSSEFDQMFPVVTNFSIENSDVSLLNLVNIAWSLTVTDENFVKKVIVQVVGDFDPQGFNTTMEYTTRETSVVIPIKKTLNSAVLCKSQNYRIKYIYTEDWLGNFGETFRDVNGDIHPFYKFENANDNITVTCIQSFSNVIPSIIDAFINHTLNATTKVPNAVVNFTVSGSLGPSLPSCYFHSYPMDFLSVEAVLLGVNSDLNTIKYSCTIDLPFRYGPQIGVSIYGIVDSLGQVVGYPTSDMPATAKEFTNIASTHQGPLIDHVWRDGNKISVTGTRLYKCILIVYPENLPTVQLSSLDIATGVFIMAGNYTPSFSSTVYVSVYNIDNLEESNRLLLIKGPPVTPTPSPTPSSTPVTCSFDCGEPQGYGKCVNGACICNPPHTGLDCKAKVDNTTVITPNPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKQ2</accession>
    <name>A0A8J4PKQ2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">S1 motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_010390</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001080">
        <property type="protein sequence ID" value="KAF2068284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKQ2"/>
    <dbReference type="OrthoDB" id="18994at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000065">
        <property type="entry name" value="Programmed cell death 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045209">
        <property type="entry name" value="Rrp5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 11 PRE-RRNA PROCESSING PROTEIN RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270:SF10">
        <property type="entry name" value="PROTEIN RRP5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23240">
        <property type="entry name" value="HAT_PRP39_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="S1 motif" evidence="7">
        <location>
            <begin position="208"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="7">
        <location>
            <begin position="389"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="710"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="545"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="10"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="552"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2068284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="859" mass="97671" checksum="9B1EDC1EB1844F02" modified="2022-05-25" version="1">MVQKKQQEIKSSSTTTAKKNSTKPESTTKVATATTTVAKTTTKPKANTETSTTSTANTVDCEVIGFEEFEILVKYNKQNNGKIHIVHSGIKESLPASQFKKGQKISNVSVIQSNQLSLVPNVLNRSSLKVGMEVDTYTLGRTSNNDLSVWISHNIIGTIDIVSNFKSLKSFLKYKESSQYLKCIIKSISNEKIELNLKEEQPKKIKAGIKVIGVVTEINTLNMKLRLQNNLSGTVKLIDTCDDGFRSTPFSIYKVGDIISTFVKSVGSKDGISLSLYSNLLGEKKVQRNPQYASYPLVKRMIVEPSVGKHVWGYVLEKTDQMVLVELTHDVLAVLLTSEIEALYVESIHVGKIIQVTVRSEPKVEGSKKMVQANLFTGITLTDKNIKVGMVLPLQVVDIKDYGVFVKTGRVNALCHIKDISDDNINAAALKELYAPGDFVIGKCTNVEYNQEKKRFIINFSLKSSLFQDVDLESISKISWDKEDNEEELVKRLPYKSLEKYLIKDDSATNSQESIFTKSSIFSAASDSTNKRKLDDTKDLYEDDDQIDEDQVTDKKKQKVEKSKSKKQQEEQIKEREDLLADHNVAPEGPQDFERLLLGSPNSSFVWIKYMSYYLSLSEISRARDIGERAIKKILATEVLEQRNIWIALYNLENLYGSPDSLLKLFQRSIQYQDPKTMYLVLIGILEGSNKFDRVEEYFKLLFKKYKQSAKIWCRYGEYLLKAGKIDEFSQLLSKALPMLPKKKQIKVISKFGQMEFKQGDIERGRTIFEGLVSNYPSRTDLWNVYLDMECRDKQRISTRDAKEKVRQLLDRCINLKVSDKNIKMFFKRYLTFEKEHGSPSTVQKVKQLAIKFVENSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL76</accession>
    <name>A0A8J4PL76_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Kelch repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_009778</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000803">
        <property type="protein sequence ID" value="KAF2068900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL76"/>
    <dbReference type="OrthoDB" id="20542at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051746">
        <property type="entry name" value="Kelch_domain_containing_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46260">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46260:SF3">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="661"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="688"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="44"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="11">
        <location>
            <begin position="110"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="370"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="300"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="370"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="387"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2068900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="748" mass="84954" checksum="D4DEA601D17DEBED" modified="2022-05-25" version="1">MAFNRNSNNNNDNNNNNNNNSGSLFDLNQITNNKDSYQCSISSCDNFIDIDSISFMPCGHQICSSCLSLEAHDPINIPNCPACDKIINHSTRFTEQEAMEAQQHKKKQQQRYRRCFLNEHNQDYEYFCTDCNEILCFTCLQAHSQHNIKECKEGLDQSEKDSLLQSFSTTQIDPLIKSFEKRIHTLKINANKYDAFHKESLRNLMENAIKYSKEISILAASRKGQLDQMGEEKIKSVKDRIEALNSFRNFIESHRPLFNKIENDQLLLDEFDQMEINSIEDQQEKETMKKTILNRDFNAMAKFTQTLNKLKQDLANDDQQLEQLLSTLKFGLKAVDQADFQWLIKMYICTCQDHYKINCNCVSKIYNSNSAKPSSNNEQVDIDKSDPSSSSASSSSKTTYYASDQIFPMDDTISKRSPLKLIIKIGGKIPNIDGSYTGCAMYDVDTRQWEQIESLSSPQGILSNSCCFFNNFIYLFTSKSSLSYGLENKMGKWENISMANYGGDGVSVVTNKSTDCIYLIGGEKSPQNIISFNPSKRNHLVFDLLLNPPRTNCFSISIKDKIYIIGGISQSNTTTTTTTTTMVVSSKNTIAPAETKANKKTISVPLTLIEEFNLTEKTLTTYTNLKVTGIIQGACYNGDDLIFIITSREFLSFSIKSKETFTLNFPPLSFFMGVSLVYGFVGSPNNECVFLIGVGGIYYFDLLVLKWFTYDTVSKDELKDSKYSKALDPNYQTYFHACIPIYQSELFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLB1</accession>
    <name>A0A8J4PLB1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SMP-LTD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_009716</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000787">
        <property type="protein sequence ID" value="KAF2068960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLB1"/>
    <dbReference type="OrthoDB" id="14198at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21672">
        <property type="entry name" value="SMP_Mdm12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027532">
        <property type="entry name" value="Mdm12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019411">
        <property type="entry name" value="MMM1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10296">
        <property type="entry name" value="MMM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SMP-LTD" evidence="9">
        <location>
            <begin position="1"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2068960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="193" mass="21760" checksum="6ADAA06EAF16C04E" modified="2022-05-25" version="1">MSLKIYWERISEKQAVRAKDHLNNLFANINKPDIIGDIKITNLSFGSKPPQFQIISISDPDPKILGSQSPSGIEARVKFSYDGDAYFMFETELIVNTPTPKFICFPISVKVSSPHFSGIASVIYDTDKVCFCLLSEDGSSPLKDLKLDTQLGDTSQHVLMNLDKLESFIVEQLREQLQKHLVFPNRITLPIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLJ5</accession>
    <name>A0A8J4PLJ5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_008485</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000527">
        <property type="protein sequence ID" value="KAF2070195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLJ5"/>
    <dbReference type="OrthoDB" id="4187154at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="5">
        <location>
            <begin position="334"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="336"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2070195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="407" mass="48098" checksum="806F38FA0137D112" modified="2022-05-25" version="1">MQSDNNIYIFNNTNDFVEKDYSNNSNNNNLFEYHLLQQQPVLQYDHIINNNYNLIDNEYTFNNNNNSNIYDDLSQVPIDNFNSNEFDCLSNHSNSNNMNNPSDCNFSHTINVGIDQIYNDQYVNNSINNNSNNQYQQIISSSSSEIDYDNDQEILQEQYQQPQQQQQDIEDEVYNNDNEESKTKIEKLIELFYSQPQNQLVFSFLNHLSTENKDKIKIFPVIAPVSKFAKVGIDFNFKSYLESQKLDRIDSVYFVKPVAYLNKHYDQEIENLDKEYQFRIQTIRRLSEQQKLVRPVVQDVEPRLEQIESIFKIIKSYQKTYTANVVLQVLKKYANEFKSRRVLDSTQGDSMNSWFYHNSSCPYPSEDEKVILGASNNLSKSQIDNWFGNKRMRDKNKRDRPSFEDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM48</accession>
    <name>A0A8J4PM48_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000478">
        <property type="protein sequence ID" value="KAF2070540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM48"/>
    <dbReference type="OrthoDB" id="1879717at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47467">
        <property type="entry name" value="OS01G0867200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47467:SF1">
        <property type="entry name" value="WD40 REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="380"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="380"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="407"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="429"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="439" mass="48685" checksum="E8DA37B60E47526D" modified="2022-05-25" version="1">MDNNSNESKNTNSENNNNDNNNLEDTCNIFTTKVKSLEKTSPASLNSCRSLILNRKNYLVASDNKLIKLNIELPKIEQKIFSKIKGIPSPVRLETSLGREAVYQTGLKHEIQSLSLSADGKVIGAIDGIGNVALYQFQDDFSLSSKSIAYQSTDTFVEIGWAGCCFNPTNSNILAKTQFYNKKIDILDGDQITQSIGLIQNPTQISYFNHERSSSPLLAVTEFNQLKIYDTKQPPSSACIQKFTPSTTWLYSLGLSTNQQGYTDLIAVGGAGRTVSVFDTKKWTNCGNWKNCLKYEITSIQFSSKDSNVCYVGGLDSEVLAGNWDGSSGVDHFTGLRVDSRWLGISKLKDQELLFGFTGSSSVYWIDSADKLFQSTNVKRPSPIYTTSSKENNNNNNKKKNNKKQPKPTDLESSPKADKVHPLKKMKTSDGQSENTDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMD4</accession>
    <name>A0A8J4PMD4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">S60 ribosomal protein L35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008857</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000589">
        <property type="protein sequence ID" value="KAF2069819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMD4"/>
    <dbReference type="OrthoDB" id="528635at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00427">
        <property type="entry name" value="Ribosomal_L29_HIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.310:FF:000002">
        <property type="entry name" value="60S ribosomal protein L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3450:FF:000001">
        <property type="entry name" value="60S ribosomal protein L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00374">
        <property type="entry name" value="Ribosomal_uL29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001854">
        <property type="entry name" value="Ribosomal_uL29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018254">
        <property type="entry name" value="Ribosomal_uL29_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045059">
        <property type="entry name" value="Ribosomal_uL29_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036049">
        <property type="entry name" value="Ribosomal_uL29_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00012">
        <property type="entry name" value="L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45722:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL29-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00831">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46561">
        <property type="entry name" value="Ribosomal protein L29 (L29p)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00579">
        <property type="entry name" value="RIBOSOMAL_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2069819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="14428" checksum="781E34132A49E4B6" modified="2022-05-25" version="1">MAEKIKAFELRDKKKSELLETLKKLRSELSSLRVAQVKSPNPSKLAKIGTVRKDIARVLTVVNQTQKTQLRAFYAKKSVNKVPLDLRYKKTRAIRRRLTAAQESAVTARTAKQLRHFPQRVFAVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMI7</accession>
    <name>A0A8J4PMI7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peroxin-12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_009116</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Component of a retrotranslocation channel required for peroxisome organization by mediating export of the PEX5 receptor from peroxisomes to the cytosol, thereby promoting PEX5 recycling. The retrotranslocation channel is composed of PEX2, PEX10 and PEX12; each subunit contributing transmembrane segments that coassemble into an open channel that specifically allows the passage of PEX5 through the peroxisomal membrane. PEX12 also regulates PEX5 recycling by activating the E3 ubiquitin-protein ligase activity of PEX10. When PEX5 recycling is compromised, PEX12 stimulates PEX10-mediated polyubiquitination of PEX5, leading to its subsequent degradation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pex2/pex10/pex12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000639">
        <property type="protein sequence ID" value="KAF2069560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMI7"/>
    <dbReference type="OrthoDB" id="107372at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16451">
        <property type="entry name" value="mRING_PEX12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000357">
        <property type="entry name" value="Peroxisome biogenesis protein 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017375">
        <property type="entry name" value="PEX12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006845">
        <property type="entry name" value="Pex_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12888:SF0">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12888">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 12 PEROXIN-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04757">
        <property type="entry name" value="Pex2_Pex12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Pex N-terminal" evidence="16">
        <location>
            <begin position="24"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2069560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="447" mass="52181" checksum="D765595B6B32FC62" modified="2022-05-25" version="1">MFLFNFNNGDPNRPSFFEMFAQFQMMPSFKPALKYIFNVLSQRHPKLRYVVKYYDEAFYSMLLLLEYHYLKYYESSFSENFYNLKRIKSSIDEKNGGSNETFFSILKQLVSTPNIEQPEVLTKSQILKKSLSMLKRSKAAAKANASQDMSIKHHDRKLSLVFLVLIPYIKTKLDDYYRRESDPLSELGLLDEDEQLDHIDQGNQLGPVDSIENNNNNSSSQTVHNIILAYTSQKIIQLKKSSLSKKLKKVFLKSYPFINALYESLFFIYQLLYLYEYTNFYTPFLHLQQINLKRLTHNDIENHNTAIANRRRDRINFVKNWYFSKLFIQIVKVLDSILDCSKYILPGSIFLFKSLEWWYSENRISAPSLPIPAPPTPAKRAPGGLAIPQDKTMCPLCLKKRTNPTICGSGFVFCYPCIFTYVTDHSKCPITFLPATTETLRKIYETE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMP8</accession>
    <name>A0A8J4PMP8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_008730</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000567">
        <property type="protein sequence ID" value="KAF2069953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMP8"/>
    <dbReference type="OrthoDB" id="5065855at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019784">
        <property type="term" value="F:deNEDDylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000338">
        <property type="term" value="P:protein deneddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044613">
        <property type="entry name" value="Nep1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46468">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46468:SF1">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="5">
        <location>
            <begin position="14"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2069953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="231" mass="26840" checksum="B74E30745A180AF8" modified="2022-05-25" version="1">MTSKDNIVISFKDATLFDSDLWILKRPNEWINDSIISFYLEYLDDQVVGKHCKEDVLLASPSAIYMCNFIETQQEIQEMFKPLKMESKKLIFFPINNNSDPTIIAGGSHWSLLVFIKPLNQFHYFDSMGTSNYSEAKKVSSKFSFLISGSNSKVHIEKTPAQQNNYDCGLYVLSIIEFLVNQYIKYDDQDIFIKEFSNQINRDLFQSITPTLVKEKRNDILSIIEKLKLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMX7</accession>
    <name>A0A8J4PMX7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mediator of RNA polymerase II transcription subunit 20</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Mediator complex subunit 20</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MED20</name>
        <name evidence="5" type="ORF">CYY_007896</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the Mediator complex subunit 20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000446">
        <property type="protein sequence ID" value="KAF2070782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMX7"/>
    <dbReference type="OrthoDB" id="1854899at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013921">
        <property type="entry name" value="Mediator_Med20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12465:SF0">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12465">
        <property type="entry name" value="UBIQUITIN SPECIFIC PROTEASE HOMOLOG 49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08612">
        <property type="entry name" value="Med20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2070782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="197" mass="22648" checksum="5E2CF58D415B15F2" modified="2022-05-25" version="1">MGYKCIFLFKGTIPDIVKRIEFLGGVKVSTWAINCSLYLERLPEGTGMAPREFHLLVFDEKPKKCFMVSRDTILETDREIISILDKANSFKKRQTTEVIGSRYEIGDFIIRLGPIVFRQEVKGIAVEVEYSACATPMTSPFNCTKLLSEFINGNLGPFPEPIQTHFDFSQYPSLPKHYSDLHTSYQYVHFFRNTNIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN24</accession>
    <name>A0A8J4PN24_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008376</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000510">
        <property type="protein sequence ID" value="KAF2070308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN24"/>
    <dbReference type="OrthoDB" id="328123at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053331">
        <property type="entry name" value="EGF-like_comC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24032:SF16">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24032">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1189"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="777"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="803"/>
            <end position="812"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1235" mass="134342" checksum="AFB1574451F05904" modified="2022-05-25" version="1">MKVLKYDVFLFIFIIIGVVYSDPVITKVTPSLLVIDLMNEVTIVGTSFLTSPKVYCNNVLLGSSNTSPNLVVAKLDNVKSLPIPTGGIYNFQVIDATLTASALYPVSSIKLPTFSQVNTVAFLKTSSSTFSQTMADSIKIGYQTPQSQLLFNNASVVSYQVTYEMAKNGWYGFYDKDGQIIFGTTVTYNPVITNYEFEGTNLVVTGFAYANPMLVFINGYTCSIVSLTDTKITCTPASDFFVYSSTNTLNLPLQLLYNNGAKQTTILNGALVTIQSYTASGTLSLVNGDNNPAGLNSKLVGMNAGGISLQQYSDIMASFQIPDDAQCGDSYLTNADGAKVSNTILICPTPKVSSFFGFAKTSPKISFTGKFLSATKYGSMSSAIEIYYSFSNGSRLDCPSPAISVDNSFTYSFSCPNPVQVYDGVTVKVVAQNIANFKSEYSFPYPMFVFIDSITSAKYGTPTQVTIVGKNFNRSLNEVKIGNSVCTSAISSMDGRFITCLFNSDVELPDPSNNLLDVVVTTAEDSETASIFSYTCTAQCQHGSCQYKTNSCGCDAGWSGDSCEKVEPSITAITSTDFGVPNKVTITGTNFLNLNLQVTIGTSVCSNITVTQDLKTITCLFKSDIYSGGALTVTVTIDSTFTTSNAIFHYSNICPGGSNGEMCSGHGDCNTQTFTCKCDNGWSNQICSTIVPKISSISPIRYGTPDLVTIVGENFVNLNLQVKIGGSICSNVVISNDLTTITCLFQSNIPVNNDDETLDVNVNIDNQFMTTNKIFSYLKQCLNNGIVCSSHGICNQQTFTCDCYKGWSTSNCSMIYPTITSSTSIKQNSPGQITIVGTNFVNLNLQVMIGGSICGNVVVSSDLTTITCLFKSDVALIDNNIKNALEVFVSINSTFTTKNNVFIYTKPDQKCPTGSNGKVCSDQGTCNQQYTCDCFKGWESGDCSLQNTGVIVETPVVNENNTTSTIVTPSGIYYDVGVDMINELNSNGDIILSYSLKNTKWIYQSGQQKENFYSTELPNKSNLNVKLTINDKDERVYYNFAGDIIPILPKSIKYQIELYNWTFSSTQNNVEFIFKSGITESNNECEKETTTTTQSTDDSIRSIQITMNGETLVGLFSDRMILDGRPTYNKVNRLTTDQISKYQLDTTPVYTSISTSYFKNSVVVDPNFGVLVSSTPDECSNSKGGIASWKLAVIIVCTVVGASFIATLTWMLLKKNRAKILIMKNKMKSIRMSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNH5</accession>
    <name>A0A8J4PNH5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zinc finger GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008287</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000498">
        <property type="protein sequence ID" value="KAF2070388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNH5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051647">
        <property type="entry name" value="Mediator_comp_sub12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007:SF8">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="142"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="40"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="152"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="163"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="22456" checksum="68078C60E12FEB79" modified="2022-05-25" version="1" fragment="single">MNVNGYYHNQHHDQQQQQQQQQQQQFTYNKLDFDEDENELQRHINSNNIFQKEQQQLQQQHQQYQQLQHEQQQQQQQQKQISLKLLCPIHRVYCRKIQPPTGKNAGKIGYICRERECSFTNWFDDEPSILKYLEKLKIDANNNNNNSNNNNQPQSIYTPSPFNNNNNNNNNNNNNNNNNNNNNNNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNW4</accession>
    <name>A0A8J4PNW4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">IPT/TIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008243</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000492">
        <property type="protein sequence ID" value="KAF2070432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNW4"/>
    <dbReference type="OrthoDB" id="24408at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053331">
        <property type="entry name" value="EGF-like_comC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24032">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035303229" description="IPT/TIG domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="703" mass="78815" checksum="46BA2F417D9A117D" modified="2022-05-25" version="1" fragment="single">MNFYKLVFLLLLVTFVSSLNQEQYDRLLEIRTNFNLEEYYPGPEPIDFCSNESIFICSAKNSTIDRIVLHGNGSSVIPSFREFVDITELEIYNASDINHFIYNAGTYLPPSLPSIVCEYCNITKIPFQDSSLRRITLLNNPLNSEFNLTKGSAFVKIQNPDPLFANYLVRIVPQYICYTSNSYTSLSIMLNESAIYECTVSARALSIQLGSFFGPETNLSNLVEETFPSLRGDNSQLELIGNNFTFNALEIASDIFSMLFYRIQFNIEEEVKQINVSNPEVFSLSSIDSRILTNDGRFLYNTSNCKNIAIRNSGLKYIYSGMFSKANIVDLRWNGIVQPFPSFFFNSDAFVQLDYNKFYGSVDKSVCGGSISFSNNNLTGLLPSCLLCYLNIPEVKKIIAGNNFANYKDTDTVYPLCDSVQITSFSNFGNIGASFHGVNFPLFNGYISGNIPNISYSIYQTQPGIIYLSYASTNYQAILKYKELVVTIHATRDFTFRFPVGKQTPKVVGSNPPLPYLNIGYSFTFLINQLLDSNVTINVDGQYVCEVNKVLPSFDTTTVACVVKSFSIPEKLLVFSFTLQNNISFSYSLQFVRHFPILTAVSSVPKSGGLVTFYGFFGNNISISNSSDIDVTIGLLPCQIKSLEDQKIVCLIEESTLSGPQNISVNCFGVPGFYPNMFFFMEDSKDCENDCNGNGFCFRSQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPG2</accession>
    <name>A0A8J4PPG2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-terminal Ras-GEF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_008064</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000467">
        <property type="protein sequence ID" value="KAF2070625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPG2"/>
    <dbReference type="OrthoDB" id="546434at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="4">
        <location>
            <begin position="488"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2070625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="701" mass="80953" checksum="2816473A44A3E03E" modified="2022-05-25" version="1" fragment="single">MGSKASKALEEAQKSHTKEISLKEYGLKTLPLVVCTNFNYITKLNLNDNVIKEIPPDIYKLRSLKIFWVNNNKITTVPDEVGLLAELEDFQIDGNQVHRLNKVFFELKKVTDISMSRNHLKEIPTDFSSFTTLKSLALENNELTALPGTMSSMVNLKTLNVGQNNIEYIFNDFSNMPALTSLTLFGNRLAVLPENFGRLGTLVNLNLRSNHLESVPDSFNMLKKLTTLSLWDNSFQDFPLPICYLESLTELSFSNNTLRFIPPEIKHLTQLRKLYLQYNQIEYIPTEIRYLVNLNILLLHHNRLASIPCEISLLKRLESINLFGNPLPAKLVQSDQQTFMACMAAELEIRKDVCSRVIQQHYRVWKKQFRFRSVVRTLVEMNKLQRNIVLANHTNQMIQSTIKIQRWYRSRKLRIQWHELVSKVLLLIQTTNNSLNRNNSTNSLGYHNNLPHSTSTGSLNNINISVARLGSLFGFDEPDSEANVTFLDNHVIKAATVTKLVEYLCNINYSEEGFMRYFFSTYPSFITPLELFQLFCVRYHVEPPQNCSPLELAQFKKTNKPRIQERVINLFEYWIKYHLNDFQDNPKLLQNFNSFITNTLLFEKETSAKRLIAIFNETKKKHIDTMAELMKLAENAPKPILPLKSIDPHAINVLDLSSIEIARQMALIDQVLLSKISASELLSKKWSKCTEENQKICPNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPK1</accession>
    <name>A0A8J4PPK1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007925</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000449">
        <property type="protein sequence ID" value="KAF2070762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPK1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="177" mass="19864" checksum="27F2E0F2D4DB01C6" modified="2022-05-25" version="1">MRILFTSVKRINIVGFVDLKLGIRPNCSNLLNFNKIKIIWNNIIDETGFQVDMERNHEVILTIGHLIPSMVISWNSFHMLHPLAGALVTGTGFTGTWGFACLGADTGTSTFGSTLSSLSSSHIDYVYPLQQYAKNLFLQPREVAERSSNLSHDLDFDNNYFEKDNNSSNDTTTTNRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPK5</accession>
    <name>A0A8J4PPK5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008241</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000491">
        <property type="protein sequence ID" value="KAF2070446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPK5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="112"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="125" mass="14852" checksum="8443D8EE504D3FFB" modified="2022-05-25" version="1">MIWWFCLPLSVSHQFDRFWFNWKYLPPGPNKVLLYHLKTPNNNHLKTNNNHIKAQDNNSNHFKPHHNKRLKTHNNNNRLKTYHKSNLKTHKPELAVRGGETQNDEANNEDPAVQNDNQVADQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPQ8</accession>
    <name>A0A8J4PPQ8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutamate--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glutamyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_008908</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000597">
        <property type="protein sequence ID" value="KAF2069769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPQ8"/>
    <dbReference type="OrthoDB" id="428822at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00808">
        <property type="entry name" value="GluRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000045">
        <property type="entry name" value="Glutamate--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00022">
        <property type="entry name" value="Glu_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045462">
        <property type="entry name" value="aa-tRNA-synth_I_cd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004527">
        <property type="entry name" value="Glu-tRNA-ligase_bac/mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049940">
        <property type="entry name" value="GluQ/Sye"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033910">
        <property type="entry name" value="GluRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00464">
        <property type="entry name" value="gltX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43311">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43311:SF2">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19269">
        <property type="entry name" value="Anticodon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="An anticodon-binding domain of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="11">
        <location>
            <begin position="65"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class I anticodon-binding" evidence="12">
        <location>
            <begin position="417"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF19269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2069769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="569" mass="64382" checksum="FFF2B134B4D9D62C" modified="2022-05-25" version="1">MLGIRSLFNVSSKRAGSSLIDIGHSGNIGGRVVLVFQQKRNYCSCGGTEHSHHELGQQQQPSQHVRVRYAPSPTGYLHLGGLRTALFNYLFAKKNKGTFIMRIEDTDQTRFVPGSAKNLENCLEWAGIPYDEGPSRPGQCGPYVQSERLDIYKQYAQKLVESGHAYHCFCSSERLDASRLNLKNKYAMSLYDRHCLKLSREEIQTRLDRGDAHTIRLKIPHEKTTKFTDVVKGNVSFQNHLIDDQVLMKSDGFPTYHLASVVDDHLMGISHIIRGEEWLNSAPKHMILYEAFGWKPPIMAHVPLLLNSDKSKLSKRQGDVSVDSYMNKGFLPEALVNFVAFLGWSPEVKEGETPKEIMELDELIEKFSLEGINKSGSVVGMDRLDWFNVNHIRKMLMDKEKVVNFFLPKIKQQLLDMNIDIANVSDQYIIEAIQCVKDRVHVVDDFQSLLKGFFVSEIDYTTEEAMKMKAKVWKSDSKANVTTFATLLSQIPDSDFNADEIYKTLQIAANSIYQGLNEKELSQKVNQLMSNIRYLLLASPVGGGIPSTIQILGKENTLKRLGDGLNKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ81</accession>
    <name>A0A8J4PQ81_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008566</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000540">
        <property type="protein sequence ID" value="KAF2070114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ81"/>
    <dbReference type="OrthoDB" id="19329at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36768">
        <property type="entry name" value="ATP-DEPENDENT HELICASE/DEOXYRIBONUCLEASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36768:SF1">
        <property type="entry name" value="ATP-DEPENDENT HELICASE_DEOXYRIBONUCLEASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035178612" evidence="3">
        <location>
            <begin position="31"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="215"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="304" mass="35140" checksum="017323614311B554" modified="2022-05-25" version="1">MNTYKSSSLIYTTTLLVLVLLFGNINQIDAYKYGDIIRFSKKSYHDLRETDWNDIRVKFSPRFQVDRTITLSSLTSIPLGESSLYKISFSLDNDRFKTSWITVADGQGTFLNHIEFKFTYSNDKIVDVKWTLDYNDEEVHHKKKPDHIYLIYRWEEAQDIDISAGLFILFTVGLSISGFLFTFIAFQFQYIDTKNKSTKVSLPFYESPLSTMGYSNNNNRDSFRNSNNSINNSINDQDNSLLNNSGNSNTYYRSSPPNTLSISHDGSNGNFDFNNNNSNNNNNKDFYDIPNESEMDSFIETKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQF1</accession>
    <name>A0A8J4PQF1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">MoaB/Mog domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_007461</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>adenylyl-molybdopterin + molybdate = Mo-molybdopterin + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35047"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
            <dbReference type="ChEBI" id="CHEBI:62727"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>molybdopterin + ATP + H(+) = adenylyl-molybdopterin + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:31331"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58698"/>
            <dbReference type="ChEBI" id="CHEBI:62727"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the MoeA family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the MoeA family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the MoaB/Mog family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000399">
        <property type="protein sequence ID" value="KAF2071227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQF1"/>
    <dbReference type="OrthoDB" id="4349954at2759"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061598">
        <property type="term" value="F:molybdopterin adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00886">
        <property type="entry name" value="MogA_MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.190.11:FF:000001">
        <property type="entry name" value="Molybdopterin molybdenumtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.340.10:FF:000007">
        <property type="entry name" value="Molybdopterin molybdenumtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.980.10:FF:000009">
        <property type="entry name" value="Molybdopterin molybdenumtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="entry name" value="MoeA, C-terminal, domain IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.10">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.190.11">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045515">
        <property type="entry name" value="Glp_gephyrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192:SF5">
        <property type="entry name" value="GEPHYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="MOLYBDOPTERIN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="MoeA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="MoeA N-terminal region -like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01078">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="11 13" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="15">
        <location>
            <begin position="7"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="MoaB/Mog" evidence="15">
        <location>
            <begin position="417"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="169"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="183"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="200"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2071227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="666" mass="72092" checksum="9C16368D64B78EF5" modified="2022-05-25" version="1">MSSYQIGVLTVSDTCARGEATDQSGPEIISMIKSHYKALMGKSGENITINTTYKVVPDEVEHIQSLVVQWVHQGYKLILTTGGTGFTPRDVTPEAIKPLIEKRCKGIEIAMLKTSLDVTPHAMLSRPVAGIKSNTLIVTLPGSVKAVRENLAAILPAVPHAIGLINSAPKASTTSEHRSKDYSSNSNSTVTSISSDGCRGHHGHSHSHNHSHQEEKPHSCGDGVHRKRVSPYPMINVDDAIDIILKQCDLLDIGTEMKPITEALGRLLAEDIHSVEPFPPFRASIKDGYAVRSKDGVGKYKILGDSLAGSSSYDPQLLAAQDPKEKYCVVITTGAKIPDEFDAVVMVENTELIADEKDHVSILSSVAVGEDIRPVGSDIPAQSLVLKKGAKIGCAEVGLLATLGLVQVSVHKLPSVSIISTGDELVDYQTKQIPSGMIRDSNSPTLASIIQELNSHFGECASRENIHLHGIVKDKVEDLERVFKDAAARSDIIITSGGVSMGQLDLVKPLLEKMGKLQFGRVNMKPGKPLTFSTITTASDSTANKNKTTLVFSLPGNPVSTVVTFYLFVVPALRKLSGFSNYNLPMVEVKLNDRLRLDPERPEYHRVTIEWDFNQHCFISTSTGSQASCRLLSLKNANALLLLPKSEGYQEKGSMVKAIVIGPLHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQN0</accession>
    <name>A0A8J4PQN0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FAM86 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006874</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000336">
        <property type="protein sequence ID" value="KAF2071813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQN0"/>
    <dbReference type="OrthoDB" id="194386at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF130">
        <property type="entry name" value="PROTEIN-LYSINE N-METHYLTRANSFERASE EEF2KMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="357" mass="40891" checksum="76F129414439EBB8" modified="2022-05-25" version="1">MSNTSNQEQDDFIFRLKVLYNQMVHIPIMLATLRKEFPKFFNQNDDADTDLIQDLKSIPLQEKINNNILNHSNRPTTSYFKKFIKTIVDQLQKLDQEVHEEILEKFMKSLSTPTGLAGDPLCIKSYCLQDSKKEEWVTLVNENAYNLVGMTTWGAAYLLSDYILANKSFFSDKHILELGSGTGLAGIALQCVADPPCKKVVLTDYSPKVLNNLKENIELNNIEIQDFINTEDFQGESEKRFSVRILDWEIEDLKCLDQCLDIVDSEIIIGADIVYDPSLARYLVAILNYLLNKKAGSMAIISSTIRNQSTFEIFQKELVEKNLIVQDITNQCDLLNPSPFIYDRSQIVLYKIYANIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR58</accession>
    <name>A0A8J4PR58_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005999</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000257">
        <property type="protein sequence ID" value="KAF2072693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR58"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="205" mass="22950" checksum="0CF452DC273BF2FD" modified="2022-05-25" version="1">MNKLQKKNPALVKVSTDDSLFELYDNYINSSSGGSSSSKTSNSNNSRKNNSTNNSDNINNNNKINNPSINLLNEIQKSVSYKLATRYKFRQQDKDKILCGDDENHNERNVPNDKLATTSATSTTKKVEKNKKEMIKKTNVSNQIKHNSSKTSRYPLSEPLFNSNTNFNEICGPEILMAIGVIQQNHLKSLINQLSSTSDTSGNRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR73</accession>
    <name>A0A8J4PR73_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_005964</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000253">
        <property type="protein sequence ID" value="KAF2072723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR73"/>
    <dbReference type="OrthoDB" id="17471at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000335">
        <property type="entry name" value="ATP binding cassette subfamily A member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="231"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="289"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="316"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="345"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="452"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="531"/>
            <end position="769"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2072723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="849" mass="94317" checksum="64657C93E1800685" modified="2022-05-25" version="1">MNNSPERASALQQFSALMYKQFLLFRKNKVVTLFRLLTPIVVMSIYAITYSSINNSSNNKFYSDQSYPTNFADQIKCCDHVVGYSYNNDPSIPDIMPLLAKTLDMDISNFVPVPQFYMTNQTGLASDSAFNRTIPNFFATVFINRVNSTQLGYNLYYNYTTPSNPMLIAQSLASYNNPVVESYAGAIQIAVEKALINTLAQNPDLQLTVRKRAAPSALNSNNFNKTISLMLLQPFAALFIYFSLTFPTIMFLNMRAEESQRKIRAYLKTFGVYDEIYLASWLLDGMIGGFYSCVVLLIFGYACPGFDFFTKTTPSILFVVFMTYSFAMNCLCLLLGSLLSTTKGAVIISVVIFTLGLFTSIATCFIGNLIYNVYGNKYAPIGAIISILPFLNINKILTDIATGATPSFLNNPNINSDSSSSGEPAPNILYDWGNFTSTPIYDEPLVKGASPISGFASLNILFALALLFLFLAWYLDKVLAGPFGGSKRFDFLFTSDYWTPRHIPFSSVDQHTLRGNINSNDPDIAQESQNVTLENMSKYALIIRNLDKEFDGKYAVNNLSLAAENGKIIALLGHNGAGKTTTINILTNQTNPTSGTALIYGYDCVNQASSIHTLIGYTPQFDVNWPNFTAREHLKIMSSIKEKRVNLEQDINNILKQIRLTSVADNIVGSYSGGMQRRLSCGLALIGNPKIIILDEPTTGVDPANRLYLWRLIKSMKKDRLVLLTTHSMEEADALSDKIAIMSEGRLAAVGTPTHLKSRFSSGFKLHIISSQPDQAVNVVRHYLPTAIFDKSTSNTLVFTIQDIGNLSNFLKYLAQDATAQSFISDWQIQGTTIEDVFLKVAHDQKKHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSM5</accession>
    <name>A0A8J4PSM5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ras-related GTP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_006934</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GTR/RAG GTP-binding protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000342">
        <property type="protein sequence ID" value="KAF2071749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSM5"/>
    <dbReference type="OrthoDB" id="10020193at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990131">
        <property type="term" value="C:Gtr1-Gtr2 GTPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11384">
        <property type="entry name" value="RagA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.190:FF:000002">
        <property type="entry name" value="Ras-related GTP-binding protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000276">
        <property type="entry name" value="Ras-related GTP-binding protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006762">
        <property type="entry name" value="Gtr1_RagA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039397">
        <property type="entry name" value="RagA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259">
        <property type="entry name" value="RAS-RELATED GTP BINDING RAG/GTR YEAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259:SF1">
        <property type="entry name" value="RAS-RELATED GTP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04670">
        <property type="entry name" value="Gtr1_RagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12" id="KW-0458">Lysosome</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2071749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="298" mass="34602" checksum="3526AD0D37E22D46" modified="2022-05-25" version="1">MKRKVLLMGRSESGKTSMRSIIFANYIARDTMRLGVTIDVEHSHVRFLGNLVLNLWDCGGQEGFLESYLTTQRDHIFRNVEVLIYVFDIESREHQKDMKNYKSCIEAISQNSKDAKVFCLVHKMDLVPEDQRDSLFKQKELEIKQNSLPLKPTCFRTSIWDETLYKAWSSIVYSLIPNVRVLEHNLDKFCKICEADEVVLFEKATFLVISHSARKQHKDVHRFEKISTIIKQFFLSCSKSQANFQAMEVRNSNFAAFIDAFTSNTYIMVIMSDPTIESSATLLNIQVAKSHFEKFIQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSN6</accession>
    <name>A0A8J4PSN6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Ribosomal RNA-processing protein 8</fullName>
            <ecNumber evidence="13">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_004876</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Probable methyltransferase required to silence rDNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
            <location evidence="1 13">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the methyltransferase superfamily. RRP8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="13"/>
    <dbReference type="EMBL" id="AJWJ01000181">
        <property type="protein sequence ID" value="KAF2073818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSN6"/>
    <dbReference type="OrthoDB" id="10258825at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2150:FF:000001">
        <property type="entry name" value="Ribosomal RNA-processing protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000068">
        <property type="entry name" value="Ribosomal RNA-processing protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2150">
        <property type="entry name" value="Ribosomal RNA-processing protein 8, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007823">
        <property type="entry name" value="RRP8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042036">
        <property type="entry name" value="RRP8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12787">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12787:SF0">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05148">
        <property type="entry name" value="Methyltransf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="5 13" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="8 13" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 13" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="52"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2073818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="338" mass="39116" checksum="ABEFDA2DF137148C" modified="2022-05-25" version="1">MKNKAPVKKDKIVEKKKTNFKKNISKLQVVKSQNNTKKPVVTNNNNNNNKKQTQEKDNNKNYKTKTIESIETPKNEDSEKYNLWNPKPSDSNKSGSVGNDKAKLSNLQEEMDNQLKGSRFRWLNERLYVSDSKEMFKEFKEDPSLFDQYHSGFKSQVEHWPLNPLDLIIEDLKKIKEKKKIADLGCGEAKLAENLSKKHNVQSFDLIAVNDKVTACDIANLPLKNESIDICVFCLSLMGTNFLEFIKEAHRVLVQDGILKIAEIESRITDSGLFILEICKMGFELIRKNDKNQYFHLFEFKKTKDTKKLKLLQQAAAKNKKNKNSKEPVLKPCLYKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSR0</accession>
    <name>A0A8J4PSR0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006148</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000271">
        <property type="protein sequence ID" value="KAF2072531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSR0"/>
    <dbReference type="OrthoDB" id="9975416at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="170" mass="19849" checksum="83FE5C7591051DB6" modified="2022-05-25" version="1">MISLKKCNINDIEQLQELCKTTYINAFHTIFNVEDVKLYATKKYSIENLRAELEDQDPQQQSYISYLIVNQDIIGYIKINNYSNKNSLDIDRLYLLDKFKGMGYGNKVLQYVESTAKENGKTCLTLTVLKLNTPAISFYEKNQFTILDYQENIILGNNSYVLLHMIKYIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PST5</accession>
    <name>A0A8J4PST5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005561</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000225">
        <property type="protein sequence ID" value="KAF2073120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PST5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="581" mass="65713" checksum="78E160B2251E5B5A" modified="2022-05-25" version="1">MDTTEMSKFYFIWRNKVLQKRIQRYNHSISFRYNESTVPFTIENTIGRFDSFDISLQLQSCVVLDLTFPNSITSLFLTEVKNTFEIKLPKESIHTLYLFNYRGSIPEGFIPSSVRHLVFNGEIDQHAIPFGVERVEDINVQLKQQFYIPNSVKEISLLCAKETVTQGWIPDSVEKLTTGFTPDQGFIPKSVKVYTNTSDLKSLACLPESTRSLTLSNDHIISSGKETIPHHIKELEIISGKSLNFFPLLPAGIEKFTYRQTNQCIENLSIGGYHSLRSLSFSCQSFNCLSLPSNLQELSIYITGSLAKGPTTKARVNLFSGNPLPRSLARLSIESYYSEDIEITSNMLPESLKYLKIRSGVRLLRGCFSNTPNLSELYLESTSPKPKVINDIPSDMIPPSITRLTLGLHRAITQGSIPNSVTYLHIKRGITEPMDIKHLPQNLSHLVMDIQYYKSSTAVRFPPKLTHFSCTVNHQGQASLMPNTITHLCLDYIKKPTTPPINQPIPASVIYLATNYLDSLPPHIRTLVFYRDPKFLDVALLKHLDSLKLSTAGSTLCHFPSTLKCIKHLPSHCISNKQEWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTD7</accession>
    <name>A0A8J4PTD7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_006000</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000257">
        <property type="protein sequence ID" value="KAF2072695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTD7"/>
    <dbReference type="OrthoDB" id="8693905at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05122">
        <property type="entry name" value="PKc_STE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF22">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SAMKA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="343"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="64"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2072695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="627" mass="70277" checksum="ED673B0C8C7679AA" modified="2022-05-25" version="1">MNLLSNIFKKKNAKNSLLLENEEFFDVEKRSVIVPPQSNKRVKKKKENQNVKYNSKDNIVVAHGGGGAGAGGGSGGTGGEDDEEEPVPTVYCVSTVGGLQKNDRKWLKLSGITEEITIENFSVVLHVLNFLRRKDLIYYKFIDQPEQIIQIKESMALGNGSVTTIVPSVVISSVKSNQLLLTTANSNSSNNSQSSPIVCGISPYCSTNELIHQNQCSLEEFIVEPTSTNSNVIINNNIINSNDNSNQQQPHLNLNHLNLHHQQIINNNNSHNGTILQQQNNTILITPQSLSAGLSHSYYYPGKKKRIDANRVFISPSHFCKYPEEIDIAIEKLLNQGNPKELYKNLYFEAKGGFGSVFLAKNRAASHASAEKQMVAVKKMPHKTLRQKRMNLNEIGFLKYCNHPNIVKLLCSFLKNEELWLVMELMEGGTLREAVGNFSFCESKIAYICKETLKAISYLHSNGLCHRDLKSSNIMISMNGEIKLIDFGLAVDFNNEKEDIHMCGSPFWMPPEQILGRPHSLPVDIWSFGVCVVEMLKRKVPNHNSRLKAMVTIATEGLSFTKLEYPYWSDQVLDFLSKCLQTDPLKRASADELLAHPFLENSCDLKEIKDILPALFMSNTLSKQGIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTE9</accession>
    <name>A0A8J4PTE9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005157</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000198">
        <property type="protein sequence ID" value="KAF2073538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTE9"/>
    <dbReference type="OrthoDB" id="809632at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05288">
        <property type="entry name" value="PGDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000121">
        <property type="entry name" value="Prostaglandin reductase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041694">
        <property type="entry name" value="ADH_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045010">
        <property type="entry name" value="MDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205:SF7">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16884">
        <property type="entry name" value="ADH_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="2">
        <location>
            <begin position="19"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="347" mass="37957" checksum="66B3806A595C5BC9" modified="2022-05-25" version="1">MVVSSNVNRRIVLASRPNGAPVKENWKLEEVPKPQPKQGEVLLRTIYISLDPYLRGRMTEGPSYVECINIGEVFGGKIAESVYVVEQSNNPNFKVGELVEGSFGWQDYAISDGKDLIKLDGPELNDPVKAIGALGMPGFTAYYGFNEIGQPKKGETVVVAAATGAVGSLVGQFAKAKGCYVVGIAGGDEKCKYAVEKLGFDKCLDHKKGDLKQLLKDACPKGIDIYYENVGGKVFEAVFPLLNVMSRVPLCGLISLYNATSLQNGEINLPGFQITLLKKRIRYEGFIIFDHWSGYDKFKKETRDLVKKGSFVFKEDIVKGLENAPSALMGLLEGKNFGKLVIQVGDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTJ6</accession>
    <name>A0A8J4PTJ6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_009507</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNR ribonuclease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000726">
        <property type="protein sequence ID" value="KAF2069176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTJ6"/>
    <dbReference type="OrthoDB" id="2285229at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022966">
        <property type="entry name" value="RNase_II/R_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050180">
        <property type="entry name" value="RNR_Ribonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355:SF9">
        <property type="entry name" value="DIS3-LIKE EXONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01175">
        <property type="entry name" value="RIBONUCLEASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNB" evidence="3">
        <location>
            <begin position="592"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1008"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1008"/>
            <end position="1022"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2069176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1031" mass="118527" checksum="DBC8FB84DBF0BA17" modified="2022-05-25" version="1">MLYVKSYLGKQCILKNIRFFCTSTNTLLDNNSSKNNSFINIDSDHFTKISKDNGVHSVSAQNILDLIYKQQNENSNSSNNNSNSNNTSINTTTIVPLQQPAIDINKSLIDDIIQYNGPDKKRFDYAIDQLNRFDIILYFQEGKLSCEYGYVLETNSSWAKIMASSFGKTTNVQKTNILARIPLSLDGDVYNNTDVIEELIDFLHELSIVYPSDKDKAIPTDLLEKIKLFTKKDSLAEFKRVEEELKTKIAPNMSIPSHLQIYLKDEPVQEKDIDIQLEVIDSKAKDVDNNETDKQQQKQQQEEGKKEKEIIYDPIVDLVRIISTRMITINRNQHKYPFQDKLRKITMQFKGMDRYMSPTTPKMLSLMNHFDEYNFKDLCRTMYMDKESFNAKYRQWFDLFHFSALANNLASSMNYQFVNSENIVLFSEHFRNEMKRKESQCKIIQDKKIFLKYICNRLQPISQSPATKLLANDDSVPYIADHNNLPKPNEKQGISYTLSQSFKDYIDLIKMVNSRGDGFNQISDIVWRELLGCFPRKPWVTREILEILNSTLKSKNQARQELFRNHLISDVNYFSTGNINTKPKPSPLEPVRTTFSKGTSFTIDSIDTKDVDDAICLVKEDNGDYRIVVHIADVSSIVKPSTRLFDWALFKTSSLYLPDHTQFMLPEKVTKACSLEPAQDNNCLSFSFKVDVHGNLYDYHVTESVVNNIHKTTYDEVNLVFSDPKSPSLKLNSQQQQELTILLELAQRVKRNREKNAFQIYINQPTYKWDAKTKKIVQRQSVDNTSAQTIVSELMVSANRVASMIATENQLAIPYRCQTKPEFDNVFAHIDWHKYHPSAFILHAISQIDQSTITAENIGHFSLGVGQYTWCTSPIRRFTDILVHTQLKAWLKTKSHVLTKEYMDLITPTISKNSLEIKAIQKKVNRDWGFCYVDQQGPGREYNATIVPPIFEGAGSTHVHLLDQGILVPLVQNMEPTNEIFKVYSMEPSNGGKLTFGTSEMKNLVKSASSSFKKSNQNSMDNKSNHEVIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTU0</accession>
    <name>A0A8J4PTU0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009340</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000683">
        <property type="protein sequence ID" value="KAF2069341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTU0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="724" mass="85390" checksum="1192F299CCDF8880" modified="2022-05-25" version="1">MNNILFFKIIKNKCLFREILKLCKQHDEIGYSFYDIPMGYLCRKKKWDIFDDKFDKYIDVINNPSRPIYNNRHLLVDFTKNDFHDFFSHNTSYSRFLKIYNFFQKDIQNLHYQMKDSINLLELSILANAEIEIIDHLYKIKPLSSYNLYTYLRQVATCGSIEIFDYLFKVKTEAHLQDLHQTCVKACLSQNYKLAQHIIDLLLNYNVNQKFEINLFPLEVIKYLIAKVDSSKLEISKIPPDYDDSIDLIMDCLKGKYNINTSKNPILDSGKASRLGNLELLQQMKKIKKKFTIMHWNGWSEASNTETMDFFLDKITISQDEFTTYAINCKDLNLLRHIIEKRGFKTFNQRIWCANSQVLEYLSSISLLPPFEADNIHGVNIDIKTLELKYKSNPSALTTRTLSACIKQKNLECVDFLLSKSIVTHVSTNALQSSLMHCDFKMYNYLKNYISLTATNPLQLIMKNDMYFWVRSQSELILKMIREEHYECIEIMQDLNRASLFSIISDHHIQKLISLPAMKKYKMMNLLFNAPTTYHKSKLFRAFVESVELGILNLTRFLIPLLQFQFSKAQFISEVNNQDHLFASRRFKHRSLLEIAPFISDTVYKQLLDRFPLLLNYKVFSFLQDDQEFKRNEFIVDLLQKAKRSNDFSLINLLASMNIKMEFDTEPFISTDIDSKTLSFLHNEKLSLSCQLSSKTLQYLKENQFLEILYPPSYLNHNKCLFEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU08</accession>
    <name>A0A8J4PU08_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003951</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PDCD5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000131">
        <property type="protein sequence ID" value="KAF2074763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU08"/>
    <dbReference type="OrthoDB" id="10252486at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.140">
        <property type="entry name" value="PDCD5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002836">
        <property type="entry name" value="PDCD5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036883">
        <property type="entry name" value="PDCD5-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840:SF0">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01984">
        <property type="entry name" value="dsDNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015730">
        <property type="entry name" value="TFAR19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46950">
        <property type="entry name" value="Double-stranded DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="98" mass="11440" checksum="2E7FD58182CE41FA" modified="2022-05-25" version="1">MEDQQQFDPEVAQRQEQQRRENEERRQHILVQILTPQARERLSRIAMVRPEKARQVEDLIIAAAQRGQLAEKVDEPKLISLLEQISEKAPKTTITVCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU13</accession>
    <name>A0A8J4PU13_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Myb domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_004807</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000177">
        <property type="protein sequence ID" value="KAF2073883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU13"/>
    <dbReference type="OrthoDB" id="118550at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000020">
        <property type="entry name" value="SWI/SNF complex subunit SMARCC2 isoform c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000014">
        <property type="entry name" value="SWI/SNF complex subunit SMARCC2 isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049898">
        <property type="entry name" value="MARR_BRCT_CHROMO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032451">
        <property type="entry name" value="SMARCC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032450">
        <property type="entry name" value="SMARCC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802:SF41">
        <property type="entry name" value="BRAHMA ASSOCIATED PROTEIN 155 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802">
        <property type="entry name" value="SWI/SNF COMPLEX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16495">
        <property type="entry name" value="SWIRM-assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16496">
        <property type="entry name" value="SWIRM-assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52032">
        <property type="entry name" value="MARR_BRCT_CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Chromo" evidence="9">
        <location>
            <begin position="12"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="SWIRM" evidence="7">
        <location>
            <begin position="584"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="8">
        <location>
            <begin position="859"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="864"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="270"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="528"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="683"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="973"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1104"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="302"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="368"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="385"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="530"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="540"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="696"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="723"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="973"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="999"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1104"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1121"/>
            <end position="1154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS52032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2073883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1168" mass="128208" checksum="11D0F68776EDC387" modified="2022-05-25" version="1">MTNNNGNGTAAATTVKVKEFSVNIKDYEDSSYFKKLDAIRAALSKTLSDDTITSKGLATLLAQFLQFYDDMLGRNATNRPNLTKIPMSLFKDYNADGSLYEIFRVMHTFRNTNDWKKFDFKAANRRDKHIEMLVSIHDSLLNLGLLKLPSVYFEDSLNNIEKLKSIVESHGGKIVDNRDSATHIIKTPLEELDEPTNVEYLRTIEHKQKMNLVHWWYYPDSYDAWLSSSDVEGEEPDVDQQIPDKWIVSPRWITDTDIFNEWMNEIDYEEEDNTNNSNSTNNAAGNNANTNTPNKKDDKSVKGKRGRKAKKKVTTPKTGTTPDQEMADSSSGTTEEGNDSNTEESNKRKSTSVPSTPSKKFKQDDETSTPATPVDSTTATTEQQPATVVTPTAATSTPAIPVSAPTTTFAQQPTSPTIPQQPATAKPLPVMMNPLVGGGVVPSPGPLIAKPVPQMVTPMKQPVPGIPNPSTPQPVAINKTPASINTHIANRPRSSSKSTKMDTPSHIAQPMVVTNISQIPAQPQFPGAQPITSAPTSTDSASGVVGASPSATTPTATTTTPTTTATGVSTPPAAKTIGLLSAFSIPPSPCSWFKMSEIHEIERNQLPEFFTGKSPSKNPKIYKEYRDFMINTYLQNPYQYLTLTAIRRNLVGDACSILRVHSFLEHWGLINYFVNPEGGSYIPLPSPQTTQSIKQFHQDKQKEKESPSLTSSGSAVEKEKESSSSSSKENETSTTSTSTTDGTVTSPSTPATATTTTTTTTNTASAAAPTIPKQLITKASPFEFRNNIYSQFKYQCNRCYHDCTKLRFCVTNRPQTLEGQNIPDHMFPMNLCSECFNNGNYPNFLQGTDFTRIEMPAPEMLDEWTDQETLLLLEALDIFADSWTDVAEHVGSKTKEQCLLQFLRLPIEDPYLEDNISKSTQITPNEMELNNPLADMSNPVMSLISFLSTSVSPVVAAAAAQAACNVLISEQDKDKDAAGSTTGEDKEKEKEKEKESDSMDTSSINGPNNTNDDEYFSKVNIQSAAAAALAAASIKAKSLSKVEEKEIQSLILKIVNVQTKKLELKLKYYVELEDGLEKERNSLEKARQNLFAERYSLMKATHTNQQPQPFMTSPNKPLSPNPVSNNTLTSPSSTSTPTTTTTTTSTTPSTITPSVIGTPMNVDNTENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU86</accession>
    <name>A0A8J4PU86_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Prefoldin alpha subunit family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005447</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000217">
        <property type="protein sequence ID" value="KAF2073250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU86"/>
    <dbReference type="OrthoDB" id="433124at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23158">
        <property type="entry name" value="Prefoldin_UXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13345">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13345:SF9">
        <property type="entry name" value="PROTEIN UXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="37"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="163" mass="19203" checksum="F69A9F81DF0D3260" modified="2022-05-25" version="1">MKKVSIQDGSFSDKELIENEKLLRERIEYIDYYERFIQNLHIDLERLMIEREKIAEDIENYSELKSNIELIKENKLNSMKTMINLGLECYVQAKVEDTNSIFIDVGLGIHVKFNLNEGIQFINQKEAYLNVKLDNYTKKINQIKTKIDLMQKGINDLNALPNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUB9</accession>
    <name>A0A8J4PUB9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_005363</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000212">
        <property type="protein sequence ID" value="KAF2073310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUB9"/>
    <dbReference type="OrthoDB" id="19132at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14733">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47457:SF1">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47457">
        <property type="entry name" value="OS05G0345500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="184"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="2">
        <location>
            <begin position="681"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="337"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="571"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="62"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="118"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="574"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2073310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="798" mass="88624" checksum="CE6CA0C5664CD1CF" modified="2022-05-25" version="1">MSHNRNNSFQQTNSINISNQTSNNNNNNNNDASSTLLSLSPSYIISTSPGSVSSFFPITYSSNSSGSSNNSSSSNNTNSGTINNTPTSNNNSNTPTFKFTQPQNLSQIIQLQVNQNDINNNNNNTPSTSPSNISSSPSSLINTPSSSNNNRTNNTKNLHKGSTLYESPLFQNGFGNYLKSFEFSDVTIIANGKSHHLHKVILSHSSKLFSDLFDRAVEHQNNEYLVYKGKDTYELKCSFVEFFESVLFYIYNGRANLTSKNALPILSLSNTFAIKGLKKVATYFLTSSITKENALSMLNKAVHIDSEDLISKSITVICKHFNQIILPNSFYNLNNNNDELNSNNNNNNNNVNNSNNSNSVQQQTITSTSTSTDDTNINTSTINTQFNIDLIENDDYEDIVNNINNSLVINNSDNNIILDETKNNINNDKINNIAIDDKSLDTSTINTIPELLSLPLNIMIRLMKQNNLSVSNEAIVYKTVVKYIQMNRAVLNEADINSLFEVVRFPLFNYQQLEEVQDHPLIPRYLVTEALLQRLKYHEGPKNELKSSTPGTNTSNSPIITQINTIGGSTLHINSTSSSSGSGSNSNPMNATSASSLNINMDIQEESSLTQMRKTPRTAYATSLEHVADFDKFGLFYYIGTNGGKEEWSNPALRGRVRVTFSSQEKGNATDCVGRTPSECWTMDVPASWISINLGSSRTMVPTSYTLRHGGNSKADCLRNWTLQGSMDSKNWTVLVRHSNDSSLNGNFSTCTWPIPNCTQAYRHFRILQTGRNSSNHNFLSISGIEFYGDLYETRSQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVU7</accession>
    <name>A0A8J4PVU7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000136">
        <property type="protein sequence ID" value="KAF2074647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVU7"/>
    <dbReference type="OrthoDB" id="24472at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140860">
        <property type="entry name" value="Pseudo ankyrin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="721" mass="84967" checksum="C9E9EF5D06573F3C" modified="2022-05-25" version="1">MNTFFIIVKNKILFNQILDKCKELTELKISFGYFDAPWGYFCRNNRFDLFDKRWNEILELNIEVIDGADGDIRLLDRYIMDFTRQDVISFFKFNTCFERFTKVWAKYQDYIQRVDLDREVLIHAIECRADTRIIQTLLDYLYQYSSHHFTKALETSVALGSIEIFTLLYKKHIVLDKYNKTGIYNELMLNACKAKHIDLALHIIKTVLFPRGYTDYDLSPLPGKVCEFILKLYNNAYCFHMSQYPPENCEAMYFKNIYPAHYVSLTEMAKINNTALLKTFFEYIKEKRMHASPAPSDLSLCQSVETANFFIDQFPNTPWNVDLVAIHSTLNVLKLLVEKGYRLFTSVALGAPFKTLEYLFDSQLLDINDPPEFKECHLFTGNLSVESIKLLARIYKQDFRVSYSFLNLFLGKQAQQSNFMDLLGCLLENDVVSPIDSHILKQITIPNKGVEIFCLIYRYLNAEQKSEFIKNIQIFTQQVLPTLLVDVNIPAIKELYSRFELKPLYKFITLKDSYHRAFECSYIYKLYYRGLEERDRLFYGNALLKFAYTTSNINLLRYLESLNISFLDTKKIEILTKSDNSKSALLFSRMIKPTVILSHFCKSNIILYNLKYLLQKMNKNSGDTDTPSIKDFYEFLIQYLIYLKKHKFIEGIKDLCQLNLEFDSSEIDLFLHSHNLLVDKDQKRFPVALLPHPTLIYLERYGLDRIFRRSVQNNQNTIFEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW33</accession>
    <name>A0A8J4PW33_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_003618</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000115">
        <property type="protein sequence ID" value="KAF2075098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW33"/>
    <dbReference type="OrthoDB" id="420380at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.620:FF:000057">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF246">
        <property type="entry name" value="TRANSMEMBRANE PROLYL 4-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="6">
        <location>
            <begin position="103"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="228" mass="25750" checksum="9774E0544516A5FA" modified="2022-05-25" version="1">MMMMLNNEESEIVKDEKSPCEKPIKLIELSEKPRVYRIPNFLSDEECTHLIESSKNKLRPCNEISSGVHRSGWGLFMKEGEEDHPITQNVFKKMKSFVNLTDSCEVMQVIRYNPGEETSSHFDYFNPLTTNGSMKIGLYGQRVCTILMYLCDVEEGGETSFPEVGVKVKPIKGDAVLFYNCKPNGDVDPLSLHQGDPVIKGTKWIAIKLINQKQQPSNNNNKEGSCAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWQ6</accession>
    <name>A0A8J4PWQ6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Regulator of chromosome condensation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004552</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000163">
        <property type="protein sequence ID" value="KAF2074149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWQ6"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051210">
        <property type="entry name" value="Ub_ligase/GEF_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870:SF408">
        <property type="entry name" value="OS09G0560450 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="80"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="203"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="256"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="369"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="479" mass="52785" checksum="4F1C6AB189409760" modified="2022-05-25" version="1">MLLLSKSHLTAAVSRPILLTKANFNLSKSSLYNTLFTLCGNDVVESLTSNYNLSQNQRYFYSTTTTTTTTTISNTNDGKNKVFSWGSGLNGKLCHGLEEKKIVIPKEIQIPNNSDSGNNNPTHITTGTTYSIIAAFDNVNNTPVLYGCGDNRDAQLIVGNGKIHSIADLEILSTKNTVQLFKDKHIVDLVSGTYHNVCRLDDGTCLFWGTSNSGQLGNPVYSRVQFDPYDNKLLSEIGIKKIGCGTTFTLALSNQGQLYSFGSSSFNELGNGDCFNERVPKKINNPLLDQRKIVDIAPGFFHNMVLDDQNTLLVWGRNQESQCFPAEDGIGKGSFCDVQILDTSILESDEKIVKIGSSSFNSYVLTDKGFIYSIGSNEQGQLGVGKSFEKGRLNKVNLPISVKTFYTGFKSIIVTDGERFFGWGSNFDHQLSLETRCVYFDPVELQNLNDLNKKFKIQNISISMSHTLSINSPSQSPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX17</accession>
    <name>A0A8J4PX17_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004428</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000156">
        <property type="protein sequence ID" value="KAF2074254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX17"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="662" mass="77430" checksum="76A1D60B093943ED" modified="2022-05-25" version="1">MQTITPSTHCNNNNNHNNGTNAFFSILRNKYINRIIVGHVLYRDPFLSLDYLFKAGRFDIFDLKWQEYTSNGFCTMDFSDHDLQSFFQHNTSYERFLKVWPVIEPFCKPKNWIQACSRCTISFDKAIITTLIDVLFRVNHLSKSTFLSYDIFKELNHAESLDIVKYFLGKKYPLPLSTTPVTHLNFEYWDELSNITAHKNLEIAKYALKELENSPVKFFLDSLSVELIQYILDNYVNQESNRVRKLTFHTFPPKQIEKALMVINHSPRVEFMVLNFNFEKAAKKGDLQFLQYMYTQVKKMEASAPSSPFDWIDPNNHQSIQFFSTQFPKIEIDFKAIEKRQSLKINKKSGKDINVLKNYIEKNGVDKLDLLQVMKHAIEKRNTQVINYLLKEYGGYMLVNESIFLEAVQDFEVLKLILPFVALQPWYDKLFIQWDHIIPSNNIDIIKLVSKGTNLITKLVDSEQSSLFFDSLTLFVPVSVFKAIFESAVTPQFNILERILKYAVRLGNIKLVYYIKGLIAKVVPLQTEFSTPDNQYLNNLCHRSILAVIEFIHLTQKGYIHIPILCPQIFNTLFDKMLTHISRTKKYYQPLYLALLDWAVIRQDHTEINRLTQTGIPVDFDDFTTFYQQKGAAQYLTLDKSLLTPLSISILDDTYDPKTYFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX26</accession>
    <name>A0A8J4PX26_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_003889</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AFG1 ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000128">
        <property type="protein sequence ID" value="KAF2074825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX26"/>
    <dbReference type="OrthoDB" id="406045at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.640.40">
        <property type="entry name" value="Cytoplasmic polyadenylation element-binding protein, ZZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005654">
        <property type="entry name" value="ATPase_AFG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038446">
        <property type="entry name" value="CEBP_ZZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018553">
        <property type="entry name" value="E2_Ub-conjug_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057668">
        <property type="entry name" value="E2_Ub-conjug_enz_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040713">
        <property type="entry name" value="ZapE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31560">
        <property type="entry name" value="UPF0652 PROTEIN C16A11.03C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31560:SF0">
        <property type="entry name" value="UPF0652 PROTEIN C22H10.08"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03969">
        <property type="entry name" value="AFG1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09418">
        <property type="entry name" value="DUF2009"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="6">
        <location>
            <begin position="576"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="540"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="638"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="544"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="558"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="664"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="681"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2074825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1196" mass="137726" checksum="C8FF12EE3B36688A" modified="2022-05-25" version="1">MLRGTITTSLNRKLSSFIFNKNTTGLFNRSKRNGQFTTTTTTNTKSINNRYIGNKQPQQQQRLYCNITTTTTLASTKNEIDTREIQNIIKSVQEEPTDGPLSLYNQMIKENKIKVDAHQLGTVKLLQNLYNQLKHHNFFISTSNSTGIEPNNRFSKLFSSIWGGSDVSLLPTNPNSQDASLIKGLYLHGDVGCGKTFLMDLFFNSIDIQKKKRIHFHHFMLDVHKRIHKWRQTKSADENDPIPPLSRQLVQEAWLLCFDEFQVTDVSDAMILKRLFSQMFDLGAVLVTTSNRPPMDLYKNGLNRQLFVPFIHFLESKCIVHNLNSGLDYRLSGTRGTQKVYLTPINDETTATLQQLFKQLTHDEPVEQKTLLLSTGRKVTVPRSSRNIAWFEFSELCEKALGADDYIEISKHYHTVFVSGVPKMNEATKNFARRFITLVDVLYEHKVKLLCTAEAAPGQLFMSDGNATEVGADIRQLTDDLTLTPEQLSRFTGEEERFMFSRAVFFGSFFKSNPTDKPLAPQKNSKKRREPDMNKDLAKILDNQEFDDIDEEDDNSSGDENNNNINNGDKMVVNDLPEGMCIECTDQPGDVFCNTCQESFCHMCCYFFHRTDKRKQHKFVITLEDTSDKEISYDQLEKITKNPGLGNAVTDNNNNKRLQEKHQQQQQQQQQQHQQHQQTTSRDDEHDHDQDIQDHDENIQDQHEDDEEQEESFIHKDDSFLSGARTLSADWFRERSKFIPIRLTLKERNDLRLLEAALHVSEYTDKIDIIHPTSGTKSKRINEQLRNICAILSGLLVASDFKKGQQLVENKDFSDNQEFFQNVFEIGRRHKIMNPAKMRTDYGKMIHLLQDGANEEVKRNLNGLNLIKPLKTVYLYLEERGGLGLLEDPNLEIATREILADNKARHEVQSEIRQKEKAIRYLAKKHSSNQLSTDEIELCIYSICDNHTYLRENRDPVKKMKVLLKKFFSPDHVDNPAFSLTLQPGINGARLSHNHRRQYNYVYQSLTLWHFILHDMFKLWYMAECDLLSENRYQLSNTGQGLNRIQQAPRVGRMMGGILRETQKKVGNEWIGSSVVHLGDHNVPNALVFIDKYTQISRILNPIVIVIEFIPKIKDPNILTYIQNAFGGRENLIKLILSDFFKHAFDGSGADNFFDAGSCIDGRLTSAWNWCSKIEKKSYYSIFLLAGFSGFDGSFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXM4</accession>
    <name>A0A8J4PXM4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004003</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000134">
        <property type="protein sequence ID" value="KAF2074689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXM4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="103" mass="12072" checksum="3A65977615F640AA" modified="2022-05-25" version="1">MNKLPSHLRSGSKWFFIEAFLFSFTLGCVIILYEMAVSMFQLTLLKWIYLFFGAIFSLQVFLFFNPINFSKIKQFFKPSSYGAQMSNESEKSHDVSMKEIRQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXU3</accession>
    <name>A0A8J4PXU3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003364</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000104">
        <property type="protein sequence ID" value="KAF2075335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXU3"/>
    <dbReference type="OrthoDB" id="24413at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052050">
        <property type="entry name" value="SecEffector_AnkRepeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586:SF3">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2075335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="814" mass="97491" checksum="8B71BCB946343C39" modified="2022-05-25" version="1">MDKYFFNLYRNLYLRKYIFSIIKQENRSLEYIPYNYYNFPLKLICSTKNQELLLQRFNQYLEKATFQYENVFGRNKSQEYFTEYIDFDFNISIYCDLLKWRELDLNLFIRIYNEFKTQILLFKKQTPFLYSHLPYDLVVKSGNYPLFLFFLEKNEPIQSEFNFLKYLADCDSIDLIQYFYSNGYLSRLNEKSIVEGFTKIFSESKSIYYPFISQWFNSNSKDQESLKILERISGKVLNVALRSNQYDFVKLVLKINSNYNYDLLPRRNSVRYNSLFSINLITYPLNSVEYNQFFFFNNFFRINLSIEIIHCIYQSYPDLFFKYLYQEPIILQNYDVSKFLLENFSNIEIAPNSFKNAISVYKNLDIVKLIAKHRPNAVFIDIDYFNTTYEIIQYLFETFPNQHFCDHFIHNGFNGDIRIVKYLDKLSKKYPDRIIFSKKSIDNAARFGYLDIVKYLYTNRKEGFSVDAISSASSGYHYSTLEFLFRELPKYEINYLLLFSPKSLVVQYPTNFKKRKKFMAIKSLWDKQRLLHCSENSQHAFFSKYAREADLSMDYFYHHPETLSMSMEKLVYKAIVFHNIPLFNKIQKVNPKLVHNSFKKKAFKSAIIQYLVKVNSVSLLKFLFKNGLLNDAKFMMMTACVENNSFLILDYLFQRGPEMSIFFNTFQKPLSFVETRHQYDINYFFIELIYNCFSLKNFTPLYYLMLLKFKPNLNLNFNCKRFESMLNRLKEILYDRLNIKTKTINSVETLAVCMQQNNNNTSNNISSASKLLINQVLSYLLESTILEQLLVEDHDHSKDVFTSNTLQSLVFTFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXW2</accession>
    <name>A0A8J4PXW2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002847</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000083">
        <property type="protein sequence ID" value="KAF2075861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXW2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2075861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="590" mass="68515" checksum="3F3AFF6D49F21E9B" modified="2022-05-25" version="1">MIRNGHFGILEEKVRRGYTLSLDLDFTELLFRVKHRDTFVALFERYRAHMTQDGHLAPWCLGQAVTDNNLVALEYMVQKRYYAQGRYNLADEVFIKKDELDPKILRLLFESRLFRPQPCTISNFTFKYSQETFDVFGEYVQKIEAFRSDTAAKEAIQAMLRVPVPRQWFKALYPLFKPVDFDIEGVDIASIEILDPDLLVYLHQSSALSTELLQKIKINLRNVRIEHVDILERAIECGIIEQNEVKDLLKLFMKRNIGLTVHLLERFPDYEMDSSEHSVLRRSIDVYKDQLVWRLLDRGLDLGANTWMGKSLLHGPPDQFIELWNRLSDGDMGINDAALESLFKSCNLKALYFILDKGYSFEKDKFFSNQHLIGLLKITHADSFDFLKLLISRSDISFSQQDFHQLLVKAGGTYCSNLAAFKIFQLAYSKSATSIDQPAILDIYKKAIVKGNLFVVRFLDNCPNLVLTLTELDVIPDVNFDMVVYFTQEHKNTYIQTILASTKYLAKAIQYNDIAVSKYLLTHYKRERIDPLVKYIAQTRNLPILVYIHNHMSTLFTAKPASFQPVYDQAVLIKNETIVNFFKNYMKKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PY73</accession>
    <name>A0A8J4PY73_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_003118</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000094">
        <property type="protein sequence ID" value="KAF2075575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PY73"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20617">
        <property type="entry name" value="CYP1_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000068">
        <property type="entry name" value="Probable cytochrome P450 508A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF417">
        <property type="entry name" value="CYTOCHROME P450 508B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12 13" id="KW-0349">Heme</keyword>
    <keyword evidence="9 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2075575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="485" mass="55900" checksum="9493F9CDC5167756" modified="2022-05-25" version="1">MIFLSLLYVFLAYLVYNYVKKFKNYGEGQLKGPMPLPIFGNLIQIGADKPHESLTKMHEKYGPIYRVWMGDVYTVVVNDIDILKKMLVTHFDKFVDRPHSPSFQFYSKNYKDLSLADREAWKEHREIVAAALTKTKMKHIYELFDQQVQDLLASMAESEKSGVPFQPRRYTQRFSMNTMLMFVFNKMVPYQEGVDSGLLKEILEPADEVFHDLGTAKLGDSIDMLKPFLTIYFKLANTCLEKVLKFIRKEYQEHLDSFKEEYKNNPRDFMDIMINEFQNRKEKLESIYLICLDLLLAGTDTTAGSIEWSFLYMANNPEIQEKAYKELDSVIGKNRNVVLSDRNSTPYVNALIKEIGRVKPIGAFGLPHACSEDIVIDGHFIPKGAQVLPNFCGISKNTKYWENPDEFNPNRFLGNSNTHGFDIFGWGPRGCVGRGIAQDELYLSIANILKKYKISSTDGKQIRDDDIFGLTIHPHTFNVSLQERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PY74</accession>
    <name>A0A8J4PY74_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Electron transfer flavoprotein subunit beta</fullName>
            <shortName evidence="5">Beta-ETF</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_004475</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the ETF beta-subunit/FixA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000158">
        <property type="protein sequence ID" value="KAF2074229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PY74"/>
    <dbReference type="OrthoDB" id="276685at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01714">
        <property type="entry name" value="ETF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000011">
        <property type="entry name" value="Electron transfer flavoprotein subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000049">
        <property type="entry name" value="ET-Flavoprotein_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012255">
        <property type="entry name" value="ETF_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033948">
        <property type="entry name" value="ETF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN BETA-SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294:SF8">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000090">
        <property type="entry name" value="Beta-ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01065">
        <property type="entry name" value="ETF_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="6">
        <location>
            <begin position="23"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2074229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="250" mass="27241" checksum="0F981B5A44C39A22" modified="2022-05-25" version="1">MKIFVPVKRVIDYAAKIRVRPDKSGVDTNGVRMSTNPFDEIAIEEAVRLKEKGIAKEVVAITMGPKASEEVLRSSLAIGADQGVIVNTDKPLNPLSVAKLLQKLAETEKPNLIIMGKQAIDDDCNQTAQMLAGLLKWPQATFASKIEISESKDSVKVTREIDGGLETLSMKLPAIISCDLRLNEPRYAKLANIMKAKKVQLTTKTPEDLGVQLNENLKIVSVEEPAKRSGGSKVTTVDDIVASLKKHSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PY80</accession>
    <name>A0A8J4PY80_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_003095</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="9">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000093">
        <property type="protein sequence ID" value="KAF2075590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PY80"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="11">
        <location>
            <begin position="607"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="12"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="396"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="450"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="561"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="402"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="450"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="525"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="561"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="58"/>
            <end position="63"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="301"/>
            <end position="304"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2075590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="877" mass="98602" checksum="3E3E09721C82AF0A" modified="2022-05-25" version="1">MKKILLLSHLKKDKKDANHSLSSSGGTGHGHHNTSKKPAMPPKTYDHFANVMVLGYHESGKTTLQRQLDLLYGLEPLDPKYYQRLIYANTLATLIRLIENSENLNVSIHNDNLERVKRVMGTPIELARNRLPRFPLKLSYDCKCLWEDKGIQTTFTHSKFCSEYRTPGRAKYYMENMFRVFSPEFVPNEIDIISAYDQVDQIQTSTLLYKRFKMDLICCSGKLSQPKEYSNLLQYQPNYIFYVVSLKDYFSNNTVIPDSTSIPICNNHTHKNLLVEALNNFELFVKSDLLDQANQVYLIFNTCDIFYENIKQFDLKHCFPDYQGGCDPTQAIEFISNKFSQFLVEKEKAFKTHVINLLDKNHVREQFDILLDSLIPDAEMRGLTFEMALSGSTNSTISGVDTTPTSGSPLSGSTNSSVGSYPSHPSSRMPHQSSILVTPLHDHLELNLNNSQMDSSSLPSSASSSMSSLSNCLVSNSTTTTTTTTTANNINNNNNNNINNNNNNNNNNISKSKSTTQPAKAQTKAIDKENKAKEKELKAKEKENKIKEKELLKLKEKEIKEKEKEEKERERAEKEREKAEKEIEKQKAAAAAPPVVKKKKGKGLSVQSGYYAMQGRRKNMEDTHAVYDDLLTETNYSANRDNIDGLCAYYAVYDGHGGADTSKALEPIVHKCVVDSTSFQSGNFEQSLRDGYEAADKKVIPVCEKSGSTGVSVMLVGNTLYAANIGDSEAVLARNVGNAKSPQYEHTLLTYKHLANDDKEKVRITELGGMIIFGRLFGSLAVSRSFGDKEYKEGEKKFVVCEPYQMTLDLTPNDHFLILACDGLWDKVTYEEAVQITTKQLKLGKTPTEISTTLAQESYEKGSVDNISVVVILLNWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZA4</accession>
    <name>A0A8J4PZA4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">CYY_002505</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">May function both as a monomer and a homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EROs family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000069">
        <property type="protein sequence ID" value="KAF2076205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZA4"/>
    <dbReference type="OrthoDB" id="269384at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016972">
        <property type="term" value="F:thiol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007266">
        <property type="entry name" value="Ero1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037192">
        <property type="entry name" value="ERO1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12613:SF0">
        <property type="entry name" value="ERO1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12613">
        <property type="entry name" value="ERO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04137">
        <property type="entry name" value="ERO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110019">
        <property type="entry name" value="ERO1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="453"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="419"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="483"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="419"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="502"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF2076205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="513" mass="58158" checksum="B7648FAC2EF804FE" modified="2022-05-25" version="1">MKFKATTITVIGLILVVSVCFSIIGSIPLKSKDTVDDCCCKKSNIDESNNNELNVLLNKIVKTRYFKYFRVNLFSECPFWVVTQLCGSEGCGVCECDENEIPLPWRIEDTTSDKVDLSPPPPGFTRWKDKKEDMWVVSPGPDSDMSYVDLSKTPEAFTGYDGSSIWSAIYNENCFTRPVDQMCLEERVFYRLISGLHSSITTHIALYYHVIDKETNTWQHNSEFFKQRFSGHPDRIENLYFTFLFVLRSVGKISNLLTEYQFNTGNQVEDTETKELIQELLQSQLMCSPNFDETLLFRDTYEKDNLIGQFKEHFQNISSILNCVTCEKCKLYGKMQTLGLGTALKILFDDNVSLSLERNEIIALINTLRQLSNSIRGINTLRKPFDQSSISFWGHEFLFNFKSVFNSIWSKALHLSSSTNSNGNSNNSNPSDSENTVDAESPFSISQHIIPTAIVKMASIIFTILIVISISLCLKRNNDQSTIINSPSNSNSGGGSSKSSKKNNEKKLKKDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-06-18" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZQ2</accession>
    <name>A0A8J4PZQ2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RIC1 C-terminal alpha solenoid region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_000503</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000009">
        <property type="protein sequence ID" value="KAF2078213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10254560at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034066">
        <property type="term" value="C:Ric1-Rgp1 guanyl-nucleotide exchange factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040096">
        <property type="entry name" value="Ric1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009771">
        <property type="entry name" value="RIC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22746:SF10">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR SUBUNIT RIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22746">
        <property type="entry name" value="RAB6A-GEF COMPLEX PARTNER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25440">
        <property type="entry name" value="Beta-prop_RIC1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07064">
        <property type="entry name" value="RIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RIC1 C-terminal alpha solenoid region" evidence="4">
        <location>
            <begin position="757"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1085"/>
            <end position="1106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2078213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1297" mass="147986" checksum="A6D4DCDF54F1EE92" modified="2022-05-25" version="1">MYFTFGWPKIYFSEIQENFIDVTHNSNCSLIAFIGSTSLSIWSGDQHRIQLGYTIRSEDSINTFGKNEKLCWSPDSSSIAIITSTGYILVYRIEREGIDVLNFKFYKEHHSPYLQHDKRKISIKFSSSFKPSVLGALCFTSNNEYIFIFSKEGYLVKSSWTGELINQFSLDIVPFDSLNDQHSGGIPSSSPLVVTKVFYNPSYSIFGLVFDNGASAILKKKSKDKLRGYWLSRENSVSIALNLKHRLAAVGLKNGDVLIYELPGPVSVSSSNNNNNNNNSNNKAAFESMNNCKYLRTFSLLQFRELTPDAIGPISEMKWTHDENCLAVGWKNRGFCLWSVYGCKLACTIPQMNDMMFSTRYIEPCKEGVLAFSFGPESYHLVLLSNGNHNGEFFQLTFLKASLASNPSLNYSERIILQTEDRLMLLNYKGKELGDIRWKHLQIPSIYLNDNWPIRHIALSRDRNQYAVAGKRGIILYNSLNKRWKMFGDRNQEQEIESQCLAWYKSVVIVANYSLMQKKHEFLLYPKQHLDNSSLLYRYPIPQNQSPALLDCNDLYLTLLTNESFFYLFKFVEKNSTIELHLVHMLSMSVPSIPLSISLLPPIQVIYNSPNTATTSNFASPSKVVVNQQQFVYCLILYASGKLCLANAENALQHELTNEIEQYWFTNIYRDKELIGNTLWAYGKSGIKVWFPFSSEEIYTNKNLHHNRSLNFDNEVYPVGFLNELGVIVGLSQGISYSSCSAYPNYEIRIKTHPFLHSILKHLLERGGAEKAWNLSNRFYTIPHFTHSLELLLHETISDDDVKLSKPKIPKIEYVVNFLKKFPQFPEVVMRCTRKIDASLWKDLFKYIGDPVALYQKCLSNGRIEIAASYLKILQNLISDDCSRKCAIELLEIVLDFDNIDLAGDLVRFLYTSDDELDKCNGQVDDDDTAVEEEASKQNRANRSKESKLFYKNLDSILSSYASKLLKAKLLRNFLLFSRKTLCDDISTWLLSKKRSTILRTVEDLDMALNSIHVQFYINLPYENIPPFSPNSVLSAFSPTPSPTPGLLPSFSSSSIGNILTTNSEDEYEDDLISLSDSIGNMNNTENTTSTNNNNNTTTTSTTTTTKTETDPTLIAMMSQSPPTMVTKHEMIESLKSRIQQTGKVDVDVVPTHQPTHVYSFKSHQFMVAPIPSGNDFNDPYFLKESIDSSMDDLYYLLQELTNAGCYEWVLVIATVLLNTTLICKTLKKVPNIYENYCKMLSHQKPVGYALLLNFVEENVYPYLQLTQNDLRKQLSSNYHMKSSSSLDFQSIHHDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZT0</accession>
    <name>A0A8J4PZT0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001663</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000041">
        <property type="protein sequence ID" value="KAF2077031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZT0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="76" mass="8238" checksum="DA295D067F3D6552" modified="2022-05-25" version="1">MSIFSALSNIANPTKSIVSKSNTSLSNSMSNQAGVNKNGCWYIIIGGPTYYINDNANPHETYFGYPPSMKNAVVVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZX4</accession>
    <name>A0A8J4PZX4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001568</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000038">
        <property type="protein sequence ID" value="KAF2077121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZX4"/>
    <dbReference type="OrthoDB" id="17675at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31494:SF3">
        <property type="entry name" value="THH1_TOM1_TOM3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31494">
        <property type="entry name" value="THH1_TOM1_TOM3 DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="18"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="166"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="383" mass="43360" checksum="5C42415880048D22" modified="2022-05-25" version="1">MVQYTHGDDYDLKWDQLILTYVILRCTGLFIVAVLCSAQFYYEVRNILAGEYKITSRTITYFCLILFPILRVMDQLSYLINGELGVGTWAWIFGIWSTFFLFAEWIFIAGFWARLLYTFFISDKIILKNINRTWISAWVFTGIYFSWVFFITGYGCAYPSKSSSLYSYGLIVCIIIFGGLVIGNGIVLAMYMRAQDKTTKKFTETVSKTLRLSIVLLVIVILTILRDIIWAGLSLPRYSPYRHIPNAIMAICEITQCVVVMYALGTPMNYLKFQVAFHSMSMSGSINTVGLSNMSTKKSGHSGHSGHSSGGGNESKEQISIQINSADYNSGHEVDDDNIDQDNVKTDCQIEIENQPEPELNVVNNDQNITQSRENLVTVPIDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0F0</accession>
    <name>A0A8J4Q0F0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial substrate carrier family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_001224</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000028">
        <property type="protein sequence ID" value="KAF2077451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0F0"/>
    <dbReference type="OrthoDB" id="434783at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051752">
        <property type="entry name" value="Mito_2-oxodicarb_carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356:SF1">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="23"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="116"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="211"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2077451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="304" mass="33072" checksum="227679593B07DF2B" modified="2022-05-25" version="1">MTGTTTTTGNAGNVPPPAARPKHNLLHNFIAGGAAGVMEICIMYPLDVVKTRAQLQVGQGSSMFSTLMHMIKHDGFGMYRGILPPILIEAPKRAIKFASNQFYEDRILQFYGNTKITQGQAISSGVCAGITEAFVVVPFELVKIRLQAKENAGKYKNTMDCVSKIAKAEGLMGFYKGLESTLWRHAFWNGGYFGLIHTIKAALPKPTTEKHTLFNNFIAGALAGGFGTVLNTPADVVKSRIQNQGTGPVKYNWFIPSAITIYKEEGFLALYKGFLPKVLRLAPGGGILLLAHSFIMKLLTNNSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q122</accession>
    <name>A0A8J4Q122_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Defect at low temperature protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_002544</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">General regulator of phagocytosis. Required to uptake Gram negative bacterium by macrophages.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000071">
        <property type="protein sequence ID" value="KAF2076137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q122"/>
    <dbReference type="OrthoDB" id="337038at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010876">
        <property type="entry name" value="C1orf43"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21425">
        <property type="entry name" value="NICE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21425:SF2">
        <property type="entry name" value="PROTEIN C1ORF43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="220"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="220"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2076137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="269" mass="30867" checksum="1A54E39B69CBB473" modified="2022-05-25" version="1">MDSTSNTNSSSTSSSTSSQEEFIVGTFYFQHFLIILFVLAAMVIISLSLIQLRIRLRNNMIDRIPRKIMPFLRPGDLSNRQRTVILNEIVKSKHIQSQIQPTMVGNSPTVGHLGWGTPGSEYAKVHFKTSIAKSWIILERAATHYNPLLAREPKTCIRSYVQFLLDNNAHLDSNIAKYYIKIYEKARFSEKEFTLDEYTAFWSKFLILIQAFEEKDTFNNINNNNNNNNNNNNNNNNNNNNNNGNNIHSNNNNNNNNNINNDIPSLYIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1C3</accession>
    <name>A0A8J4Q1C3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_006005</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="7">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000258">
        <property type="protein sequence ID" value="KAF2072671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1C3"/>
    <dbReference type="OrthoDB" id="15796at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008240">
        <property type="term" value="F:tripeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04056">
        <property type="entry name" value="Peptidases_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11377">
        <property type="entry name" value="Pro-peptidase_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000040">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000107">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015366">
        <property type="entry name" value="S53_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030400">
        <property type="entry name" value="Sedolisin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050819">
        <property type="entry name" value="Tripeptidyl-peptidase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218:SF40">
        <property type="entry name" value="PEPTIDASE S8 AND S53 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218">
        <property type="entry name" value="PROTEASE S8 TRIPEPTIDYL PEPTIDASE I CLN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09286">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00944">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51695">
        <property type="entry name" value="SEDOLISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035171306" description="Peptidase S53 domain-containing protein" evidence="8">
        <location>
            <begin position="19"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S53" evidence="9">
        <location>
            <begin position="204"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="277"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="615"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="660"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="661"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="683"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="685"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2072671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="708" mass="77305" checksum="CCD6FEA299FBA44D" modified="2022-05-25" version="1">MKIHLFLSILFFIALLEATTHDRVIIAHPKPPLHWKPSHILPDPSELIQFKLLLKQNNLDKLNQYFWEVSNPKSPYYGDFLTSQQVNDLVRSNEESFQQVYQLLGQHEIHQDEIEEKADYILVRTSVERAASLFQAKFLKYKSSKTGAVRVRLHGPATLPSHILDHIDFVTGLSEFMDKIKTSHHNPAKNYDNADKASLNDDILITPPVLKSYYNVPPTEKGSAPSNFQGIAAFSDYFSQGALDQFVKTYSLQTPNVTINGSDCLSGGCDQYESDLDMQYISAMGQGIPILFMAHPDGEWILDYAQSVLYMHNPPLVQSISYGWAELSQCDITNGCSTYGYNSQQYVNRTDVELQKIGSMGISVLVSDGDDGAPSLGGSSGNCPIDPGTYCPTGGCAHTKTQCAELTFSTSDGSLCFFPQGIGSDSCSSMLNDPNLQTAMNAFSDANTKCKLAFESDRTNLPHVYSECACSSLSNVTNSGYTVSAYSFSESNGAIFTSEYPTSSPFVTSVGATQFLSRNSHITQELGCSILEGAKITTGGGFSTFQPQPDYQAKAVASWMANNQGSIPPSFAFNPTMRAYPDISFNGHNYNIFASTNSANFDQCPCTQLPVDGTSCSSPALAGLISLINDKLISAGKSPLGFLNPLLYQMALENPSSFNDITAGSNNCNRSYCCKYGYTATAGWDPVSGIGSPDFEQFEKYILSIKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q219</accession>
    <name>A0A8J4Q219_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000033">
        <property type="protein sequence ID" value="KAF2077290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q219"/>
    <dbReference type="OrthoDB" id="47172at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461:SF103">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING PROTEIN A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="1">
        <location>
            <begin position="101"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="255" mass="29140" checksum="584C19FCAE9BDD1C" modified="2022-05-25" version="1">MVLVQELERNEQSVIDQEYFYQLIDENKPVVFSSFAKSWDAIQKWTPSYLVEKVGDKQVDVSMCTFGPMSDIFTMTFSQYIEKATKNEFVIDESTPIAKRPYLRNFGLFDECPELKNDIKSAEGMFKNDIHNLVIQGAFIGSQGSTTVLHKDTADNIVSVVSGAKFIAMIPPKDETKIQSCHQDDEISVQDIYDKKSQDTYQSRIENHPVFANVENVRYTILRAGEALYIPLGWNHYVSNLEFTVSVSCWGKKMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2B9</accession>
    <name>A0A8J4Q2B9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Paramecium surface antigen repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000047">
        <property type="protein sequence ID" value="KAF2076842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2B9"/>
    <dbReference type="OrthoDB" id="5912242at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052326">
        <property type="entry name" value="Diff-Dev_Assoc_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33459">
        <property type="entry name" value="DD-GDCA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33459:SF7">
        <property type="entry name" value="DD-GDCA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035316314" description="Paramecium surface antigen repeat-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="36527" checksum="397D67435CB82BD5" modified="2022-05-25" version="1">MKTFITLFILVLVFWISFNGAQDSHDHDHEHEECFACKNLNDYDCKGCIDSMCSPVAFNSTEYQCIVPKGVGEECIPSQCKTGTVCSGYSETHNIKGVCIDGYYANLGEYCSKNTDCTGYPGDQSCVDGKCSNLKKDCIGDNNCGSGQVCSNQTCIALLKSGDMCNPSVFNCPYTDFCHLSTKVCTKFYTGKLGEMCGEVKHCDIAENLNCINKTCQKIDTTVCSSDNCLSCSCDGKCMIQSFGLKKKDATNKYLECIQDNSCKLVGNLYSKNSCIKQNCAKEFCAYEQSYDTGIFCGAKVVPACEGVEMSSSTKPIYSVLFTFIMVLLVILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2I5</accession>
    <name>A0A8J4Q2I5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_001852</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000047">
        <property type="protein sequence ID" value="KAF2076824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009927">
        <property type="term" value="F:histidine phosphotransfer kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16922">
        <property type="entry name" value="HATPase_EvgS-ArcB-TorS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000010">
        <property type="entry name" value="Sensor histidine kinase RcsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047:SF72">
        <property type="entry name" value="OSMOSENSING HISTIDINE PROTEIN KINASE SLN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13188">
        <property type="entry name" value="PAS_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="10">
        <location>
            <begin position="272"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="11">
        <location>
            <begin position="406"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="473"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="9">
        <location>
            <begin position="1190"/>
            <end position="1310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="326"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="964"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1050"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1108"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="964"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1068"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1108"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1137"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="6">
        <location>
            <position position="1243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2076824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1324" mass="150048" checksum="0129380156734B6E" modified="2022-05-25" version="1">MSSTGSSLNNSNSNNHQHRQQPQPQQQHQQQQQQQPQEMITFSFEEEEVDMVNECKCKCCAKKYNNPPPVQDNVQSDFNSQFRDSNPVMYNELLYDLFNSVPIMLGVCDLVDLKNSGFENPNPNEYDLKFIISNKCTFDSFRSFAKDKPLGSTSIDNYYSVKKLDLPLYFRTMWVQHMLKSLKRNKIVKFMYPRYSDKGDPPSPEDFYSDRIIWKKSTVNHMGWSENGDPRFFFTSEEVTKEKFQKEQLVNHFKTQLETLENRIEQRVDEGIETRFKYVLECIPQMVWITNYQGKIEFVNRKWKDYLGIDNSDQYLNWKSISFNPDENNNNNHQNNNSQTTTTTNNNSTSLNSNSLDPSNQFTSNNNSNSNSPSLNSNQNSNQNNQNMVVDFDFLWKDSLREMKRFETEILLKSISGEYRWFLVRADPFIEPGEIQSTSTIKWIGTCTDVNDQRTAQERIENAEKSKAIFLQTMSHEMRTPLAGIMGMNSWLLSSSPQTLTGDQMDCCHTINMCAEALLVLINNILDLSKLEENKIVLEETEFHPSKIVEDSVDILSTQAETKKLDIIYQISYNALPKVIGDFYRIRQVLTNLISNSVKFTPINGQIIVGCEIFNESTTNKKRFSMDLIEDAEKTNIKGRYGKLKFWVIDNGIGIPEEGREKLFQTFSQYDASTTRKYGGSGLGLAISKRLTQLLGGDIWFDSQKGKGSSFYFYVEVFFPDDHQKSNSNLLLSPNSSSNNNINSSSGSLSSTSTPTSSSTTFNNFSFSNSNILSAAATSTFSNNAELNSLSTKIFLLSNNKTLVESLAKWIRDWCTSQSVEIRFNLEQIEEITKKSISQGKKLDYLFFVEDSYTTQFSETWSFSQNYLLPFHQETLSNSSSSSTNNNSNNSNSNISPMHNIKTVVLSFSNTNYPNATLLKKPIKYHHLKECIFSHLLFFKTIYSLNSLEIIKNIISSQKNNLTQPKITINGGSSGSSGSSSSSSSTSSSSNINSPNSFINSPKNRKNSILINNNNSSSSSSSVNHNGSNNNSKDINNNHEDDVLLNLDENQIENSKKRSFDSSTSEYSLNKNNSSNGSNHNNENINAKKLKSEELELMNHIISPKSSFTTTTTTTTTTTTTSTSTSTSKSPNLLSINNGNNNNDNNSNNNEITQATVGCIPISFKRERRNSVVNDTDIPLEMTGTRYPLKIMVAEDSQVNQKVASRFLTKLGYKKEDIVFVVNGKQAIDYLENNEMVDVILMDMQMPELDGCEATNRIRINYPTTGPHIIGVTANAFNEDKDKCLLSGMCQYLAKPVKLDVLAMELKRAWLTRSNHKVCLCKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2W4</accession>
    <name>A0A8J4Q2W4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GBD/FH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_000816</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000017">
        <property type="protein sequence ID" value="KAF2077854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2W4"/>
    <dbReference type="OrthoDB" id="427644at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056771">
        <property type="entry name" value="FH3_FHOD1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014768">
        <property type="entry name" value="GBD/FH3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45920">
        <property type="entry name" value="FORMIN HOMOLOGY 2 DOMAIN CONTAINING, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45920:SF7">
        <property type="entry name" value="FORMIN-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24959">
        <property type="entry name" value="FH3_FHOD1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51232">
        <property type="entry name" value="GBD_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBD/FH3" evidence="4">
        <location>
            <begin position="47"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="557"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="347"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="557"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="582"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="611"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2077854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="621" mass="68109" checksum="8050078A6A38B8A3" modified="2022-05-25" version="1">MLLTITLQVDPINGNTGGGNSLTASSNLGGTTTLTKSQRASINVSTLHTSSGSKTYNLDANSSYKTVFLAICQLFGISEANSSEYCLQIESNKKYLNWPPSQDEFKNKQIIKYLLENGDSSLLLKFNPSFRSIKWVSALGSQDKEKDIIFHLKFKLQEPEFAESFIEQNGMEGILRVVTNSKGNAQTYALASLRACLEYISGMDVLTKTPHLIKQLFSLVDSSVVGVVRGSLELLFVLCTFRKEEGFKSIHLAAKSTAQAQGKKPYSDIIKLLDSGDLETKINAFTLLNVLLDNCPSNEKVAKMVKKWGDLGLNEKLRTLTDIQQQEFQVQLEIYETVSGVNLRTRASRLEAICKRLKSKITEFEAQQPLIAILKEELKLSQQLIKEASTDRIFLNSHPMQRYMGPLITNYPNDLSFLKTTQIEREKTSEFDKRIALVSEQLRQETKLAEELKNQIISSKKVFDETIIDMTVENQRLTASEIKYKMELEALKASGSSSSGGSSTATSDPATLESLNLANLEIQRLKLVIEERLPPNEAQQLISSSLLINSEKLPTFTPVSPDSSDSTDPAAAAAVVDGGAGAPPPPPPPPPPPPGGAGGPPPPTSSSPSTWQQKGCTSRPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2Y9</accession>
    <name>A0A8J4Q2Y9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription initiation factor TFIID subunit 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_000319</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TAF13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000006">
        <property type="protein sequence ID" value="KAF2078335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2Y9"/>
    <dbReference type="OrthoDB" id="10266074at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07978">
        <property type="entry name" value="HFD_TAF13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003195">
        <property type="entry name" value="TFIID_TAF13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380:SF5">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID/SUPT3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02269">
        <property type="entry name" value="TFIID-18kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2078335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="105" mass="12640" checksum="92CFD9F4E02FD0C6" modified="2022-05-25" version="1">MSTKRKRMFSKDLKHMMYGFGDVREPLHESIDLLEELVFEYIQEMTLKAAQVSSKRGKFQTEDLVFLVRKDPKKYYRVIELLRMNEELKKAKKAFDETNVEEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3K5</accession>
    <name>A0A8J4Q3K5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SNF7 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_000036</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000001">
        <property type="protein sequence ID" value="KAF2078665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3K5"/>
    <dbReference type="OrthoDB" id="17380at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1060">
        <property type="entry name" value="ESAT-6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF10">
        <property type="entry name" value="GH13992P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="24"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="117"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2078665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="218" mass="24995" checksum="B230077D8DCB57AF" modified="2022-05-25" version="1">MKFFGRPKAQTSSQDTISKLRLTLEMLDKRQKFLQDKADREELEAKKLVTQKRKREALLCLKKRNNFQNEVNKLQGSYDTLSSQIFALENAKMNMEIMNSMKEGAQTLKDLHGHLTVDKVDDTIEEIQTQMEIHEEISRAISQPLGTQLDDEDDLLRELAELEQEDLDAQLLQVTAPSRETPVVNNAATIKFPTASTVKPEISKEEEEYRALQESLAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3L0</accession>
    <name>A0A8J4Q3L0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Purple acid phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_001242</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.2" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000028">
        <property type="protein sequence ID" value="KAF2077469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3L0"/>
    <dbReference type="OrthoDB" id="45007at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00839">
        <property type="entry name" value="MPP_PAPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.380">
        <property type="entry name" value="Purple acid phosphatase-like, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041792">
        <property type="entry name" value="MPP_PAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025733">
        <property type="entry name" value="PAPs_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015914">
        <property type="entry name" value="PAPs_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008963">
        <property type="entry name" value="Purple_acid_Pase-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45867">
        <property type="entry name" value="PURPLE ACID PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45867:SF10">
        <property type="entry name" value="PURPLE ACID PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14008">
        <property type="entry name" value="Metallophos_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16656">
        <property type="entry name" value="Pur_ac_phosph_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49363">
        <property type="entry name" value="Purple acid phosphatase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035342707" description="Purple acid phosphatase" evidence="3">
        <location>
            <begin position="20"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Purple acid phosphatase N-terminal" evidence="6">
        <location>
            <begin position="23"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="4">
        <location>
            <begin position="131"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Purple acid phosphatase C-terminal" evidence="5">
        <location>
            <begin position="354"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2077469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="426" mass="48684" checksum="AB1743F72F180729" modified="2022-05-25" version="1">MDRKITIFIILAFVGIALADLDPHHVKLALTKQTDQYRVTWFTNDQGSSPSLLYSTNTFQPQSGAGSAAVFGVQGSVESFDTPHWNGHSNTAVMEGLSEWTTYYYSVGDKETASWSQVFNFSTRGFTQTPFKFAVYGDMGHGGVGIDRDENYTLSGVVQRESELDFVLHVGDIAYADLKLSTIIQGNETVWNEFLDIIQPVSSILPYMTCPGNHDIFYDFTVYRKTFFMPTDKPTQSWYSFDYGQVHFVSFSTEHDYFPLSSQYNWIENELKTYREKNPNGWLVVYAHRPMYCSVKWKWCKNKHESRKLFVKTFEKMFFRYNVDLYIAGHVHNYERSLPVYKGEVAGSYENPKGPVHITIGTGGNKEGPLHGWQEAPEWSEGIRFIGTGFGTINILNSTTLQFDFIGNEKNDVIDSFTLTKGDFFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q428</accession>
    <name>A0A8J4Q428_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000234</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000004">
        <property type="protein sequence ID" value="KAF2078484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q428"/>
    <dbReference type="OrthoDB" id="10394449at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="533" mass="60924" checksum="30131AB27E09E1DC" modified="2022-05-25" version="1">MTGNIDLFYKVYRNTYIRNIIRANVLRDLIINVGNLDYLTDNHNYLETLSTIDKIHYNIFIRFEINDPDTFAKYTSHPYRHLITCLDLSFNIFQHMEDDVFDCDLVPQEVQMLSFYTPATQKGKGTLPQSLTDLRIRNIFSNSKSWLIDDIMANLPRDLKYLLLPSYDIEAPGCVLPANLSRIDYKSNVGCLKNLVGPTDRVIEGIEVQVESAEDLDWIENNKWVNSINTFRLNFADIQIPLHIRKLTGYFEVNTKDVPSFIESLTCNSLNGSLVSPNIKHIRVSEWLGKLEKGILPVNLESLDISEYNFPIEDSVLPSNLQVLNLGTFDQPLELSALPNSLTKLTLFQFNQPFPSLPNQLKVLNIPQFTQKTIPIHLIPSSLTRLELNSFDGIFENAENGSYQFNHLVSLSVHNINKSISNILFGSKFIKIGFGAYDNDVCLQPIDPSIRNLCLFFKGESDINLYPNFLPKSLIKVQLVVFDFFFNLETNSIPEGCKFLEINNPIDTLNQEVLPKSIKSIKCIPNPFIHDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4K7</accession>
    <name>A0A8J4Q4K7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000600</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000011">
        <property type="protein sequence ID" value="KAF2078129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="778"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="851"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="992"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1060"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1260"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1401"/>
            <end position="1455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1480"/>
            <end position="1526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1541"/>
            <end position="1619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1631"/>
            <end position="1653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="782"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="992"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1103"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1142"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1169"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1196"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1271"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1296"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1418"/>
            <end position="1435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1444"/>
            <end position="1455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1496"/>
            <end position="1512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1513"/>
            <end position="1524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1541"/>
            <end position="1552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1555"/>
            <end position="1585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1597"/>
            <end position="1607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1653" mass="184905" checksum="C7EC3B96596FA6B4" modified="2022-05-25" version="1">MIPSNSRRELNRDDSFMLKHCKKFENSIKNTSTYSEQEFNNLNDIVSFVLDSTEQYTKIKTNGIMLVGPSGSGKSTFLDFLSGAQINVENDGIYARNPIQSCKLNSFLPNLYSPCDISGYKDTTFIDYPSNDKNGPLYEILKESTKLKIANSCSNIKILFVIQQSDLTDGRGGIILGQTADKLKSLVGDISDLYENIFLVVSKADPSQDILEQINACLNHINKQDNGELGNFKPILQEIIDNNRFTAFCNPPVYSTDNDLDYVPLSDPDLSRETLLALIEKLEYRDIKGSNRFLVKSSPHLKNMVDGYYHDGPILGAISIVQRNVIEQFNQYFEKISKQKASIDIVNIPTFLSTLEQINDFINLNIDEITFQDFCYQLISKLLPLENYFPSSKIPLDDINEALSIPAFYSKFSSNPFQTLENFNFKELFQFNKLFTSISNDLNSIKQPVYKIQGSDLICFGYDLRFSDVVSKVDNNINNIEIIALNQIDIDHDLTGPSTNLSIYSPVWNITRKCEINLSATSNLIPAGVEESNDYLNNGPARGLPGLPGSGGGNFYGVGQVFAGIKNLSITSNGGNGGTGGSGEVGINTGIPINFLNPSTVNIQGQMVDSTNYEIQQSEMNAPIKNELLVNFMKQHWITNEGTFIAFIIKDKFYGLSNVGSKINRFERILFDVDIERIGDEDYPGGVGGIGGLGGHSGQVCVLNLNDNTDITNQISVIRLNGGNGPNGIRGKYGPKGIGKKEVKITFSHNFLPNGSSNPTATLDLLLPETVSNAPYPRSLFASFNSDNQNQPEPHPNNAESSKNSFSTFITEKNQAFNHIDIMKSTFIKKIIHSDTEDLIDDGDGSISSLSTSSLLESNESDDEFNPQEIVSPDDLTFSQDNIPEPTLVDEPMHPDLFVSNLEEDDENQDDLYLSNLFYISEPIVENENLLESNIIQDVEKDSPSTILVEFDTVEKNLDINIENETIQQTIEALPEVKDAIHHVIDTLVKSNSFGSESESESVSSQEELLSDQEKESTEDPIQDKYCIIETQVLENEPMETEPTINLESTLDIDGKQISVESEPGSSQEEILSDQDSQENVSSENCIDKNNQDEKDIDEALESQDQPINQESTLESNILDETIVVESENESEPSFSPDELDQESKELAENPSHDNSMIEFKEDEVLESQDPTINQESTLGSDLFVEKEISEDESESGSSQEELLSDQGCQDEKDIDEDLESQDQPINQERTLESNILDETIVVESTEDPIQDNYRIIETQVEEDEPMETEPTINQENTLESNILDESENESGFSPKKLDQESELTEDHIHDNSMIEVKEDEVLESQDPTINQESTLGSDLFVEKDRETVAQSISHDSDAYIEDCIQDDEKNIPDKYFTQEDEEHIPEVETKEAECPLHDSLVDNLVDPPQENNTIVETPKDQVETLSHHPYHYEDSGAPFENTKPSESKDDIDSKQENIVVLDNLLPDAKLSQESISLLENNSYDEMQDDKLESESVSESESSESELSESDQEEKSIQDNDIKYSPDIIVEVDSDIVEREEPIVIDQKEPIDVHQQPTPKYNFSPVQDYSRPTNFSKSQTLLTPTPTRPISKPPSTPLTTLIKNQIPLNPPRPSTPKKSISERISMFEKLAKESQQTVTIKSPAPKRSLDQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q7G0</accession>
    <name>A0A8J4Q7G0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_002855</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000084">
        <property type="protein sequence ID" value="KAF2075821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q7G0"/>
    <dbReference type="OrthoDB" id="17063at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="274"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="43"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1176"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1222"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="43"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1222"/>
            <end position="1247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2075821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1253" mass="139210" checksum="2540943537961B23" modified="2022-05-25" version="1">MSLKIGVCSSIKIANTASTTYHSPASASISGGQHNQYNHQYSQNAHHAGSSQGQDGQDGNSTGITISQTNSNVNIGDQMAPVSNNIVLIGGVGSTINSSDSIGSSLSSNLSSLQYTPSASPSVGMGPSQQSYYTQSIIQSNLQANQIIFNTVCFHPKSPIMYAAIRNEIYFYDFISNSVVGKLYVDHNETIKHLAAVTTSSNTVLLLGLTEVGVAYLWDPETNKLLKIVPQIKEFDTRTITAKCIAPNKPTIYFSKLNSKDIVVVDFKTLPYKLKGHKKPISSIAHHPTKSLLASCSNDGSLKIWETRTQMSYPSSFEDFASYENSRNIEHSSNFFLAFESLTGKYMIMVGSSGLTLVYGDLSSNNPQEIIANGFICKGNTIMSVQHHPHLAVFLVLSITPAGNEEITCWEVNISNKSIVPSSLIPTFIPETNDPFSYLPKNSKPLSVPKLTATHLVLHPTKNYMTLQWEVSPTFNVSISPLAPNLNSQSFASVRHVYQQIYSINSFDSLNHSFPLVSNVPIPMGFFFQPDGTFNYPTEITFFDGTYVKCYYPLNGTTKKLIEQPVINNNADEITKPKKFLYNEELQLFALIYDSHSLTTQTQLSKYTISDIHGSFSQQGDGVDCVFMNGNSAVGHQILILGFDGKLAKVANVTKQGMSAFKNHTLTPKITSVHQTPLQDGRVVLYFSQEKACLYYSKNLKPEFKDNYAVDCSSDSPSLHLYPNERVFKVEWQSDTLKSGQNICAILTDHRIMITNAQLKIINQISVPPNHKSNNAYFHSIFWLEWTLLYTTPTHLMYTTLQNNIPPRPITTLSISPIILSTILPDRMIFGYQGQQVPGRNETSVRCQAVGLLECLIFGVLSLPPFIQFGEKKFISSCLQNVVSKFDYKRLSRSVLDKLREKSFIDLAYSLANEMHSSQSKQTLLDKFKLAWTSKQYSDANKHLSEEFARMLALKSPTDTEKRQLAKLKENMRDFGRECMNAGHYTLAKECFTKLQENVYLLQISILLNDRDSVISIRSDAESKGDIVLLAAADKYLAKRASANKVNPPVVKILPWEPTPTINLGIKVGVDYLAPINLNSITRYYPISMPFSGAAPSLNNQRHKLRAPDELWPPQDYKYSVALSPPRTLMSLVANRLSTKSHMSSTQTLRRSPSAENVLKVGKDFPIKMGANIEEYDYDDDSSDDSGADADVDSETEEDLVIVTKSLEQQILVVVENNQEIINNNQDSNQNNIDSSNSSTPSTNSITGGEQDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q9A8</accession>
    <name>A0A8J4Q9A8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Acetyl-CoA synthetase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_001521</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000036">
        <property type="protein sequence ID" value="KAF2077201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q9A8"/>
    <dbReference type="OrthoDB" id="10253115at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031956">
        <property type="term" value="F:medium-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05926">
        <property type="entry name" value="FACL_fum10p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000008">
        <property type="entry name" value="2,3-dihydroxybenzoate-AMP ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045310">
        <property type="entry name" value="Pcs60-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201">
        <property type="entry name" value="ACYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201:SF5">
        <property type="entry name" value="MEDIUM-CHAIN ACYL-COA LIGASE ACSF2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="5">
        <location>
            <begin position="41"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="6">
        <location>
            <begin position="449"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2077201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="539" mass="60347" checksum="3B6968E3E021A1F8" modified="2022-05-25" version="1">MTKVTLYEIFKNTTTKNPTKTLVTIPSQQLNTNTNEFSFLNISYQKFLNDSTSFANKLQSQSFKPNDVISIVIPNSYSILSTFMATTFSKCIAAPLNSNYTKDEFNFYFKDMGSKLVIIQKGYKEAIEACIELGIKYWVLEQFEQFDQQRLYYTIKENEETIFTTETIGSNEFIKLDNEPEPNDVALFLHTSGTTSRPKGVPLTHENLATSSKNITDTLHLNEQDCSLVVMPLFHVHGLIGVCLSTFYSGGSLVVPVRFSASVFWNQIKQFKVNWYSAVPTIHTIVCSIQSSAPESTKMSNKGILRFIRSSSSSLSPTLLGTLETFFGCPVIESYGMTEASHQMASNPLPQDGTRKPGSVGRGVNVKISIVNDAGDHLEQGQAGEVCIQGKNVMHGYHNNPQANIDNFTKDGWFLTGDIGFLDAEGYLFLNGRKKEIINRGGEKISPLEVDNALLENDKVLEAVCFGVPDEKYGEEIWAAVIPKEGQELTVEELNQFLQKKLVAFKIPKKIILTTEFPKTASGKIQRRFVSEYFLKNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4QAJ8</accession>
    <name>A0A8J4QAJ8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RasGEF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_000720</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000014">
        <property type="protein sequence ID" value="KAF2077996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="20818at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF99">
        <property type="entry name" value="RASGEF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2 3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="6">
        <location>
            <begin position="874"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="5">
        <location>
            <begin position="1340"/>
            <end position="1580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="23"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="202"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="297"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="352"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="538"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="587"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="635"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="667"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="744"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="109"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="316"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="352"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="371"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="538"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="598"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="618"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="638"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="681"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="744"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="779"/>
            <end position="817"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2077996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1603" mass="178962" checksum="29E9388762CC4158" modified="2022-05-25" version="1">MNNINNHTDFDLYSTFTGEKINNGTSSNTSSGEGGGSSGATTTTTKNIPQFLKIEDRTNTTPTTTTQPKSVATAAIGNNVNTNTINFQPLASIRLNSSSLASTSNHREEEEEEEVEEEEEEEFEIKRITPRDYNNSGRGLLNSSILQSSDSLVNSTLPKSTTPISTTLITSSSLPSTPSLISNSNNNNNSLIKPSAKWKFASSPGKTRPLSVYKPIQGSPSVSSYPKPTFCLDKEAQLIPDYTNNNNSSSSSSPIVVLQGPRSSVIEIQNNSNSNNSSGNEQEEQQQIYSNSLPASMFHKHHNHNTSHTNDESESHLSSSVLTSSNGTPMLSRKSTLIDKVKYIFSPDLTQNGGQLFQDTPQQKEQQTTATNQLPPSQQPQQQSNPTTNTKDKFTINIFKIFEFPTTETIISEHPCAYRRKVSKAGKLYITDNYFCFYSLIFNKEIKKFAHFKDVLTIRKVSSIIIGQSIEISTIYKKYMFGVFENTEVAYQALTNQLSQYKSLTEQRNKSSINTSSVSVISDQKKRSRFFSISTTVSQPVNSNSNSNNSDKKKLSSSTSTYNLNLKKINDSSEDALVAPQTARANVTFSVENNSNNDGKDSGKDNDSESSNNSNNSTPSNVTPQQSSPITIIKSHSIMRTRSGSLPSYSTKKSSKLNYLISPQKNLDKLESTSPSENEITPPSSVGSGSNGDSNPNTTTTTTSTATATKPIISHRKNNSAHKSITFSPNILANLFNLNNMYEASSTTPNSSSPATASQMTAVDNEKDSSTVNTNSIPNSLANSVDSLVSNSSISSTPSSSTPPVVQPQQQQQPLTPTRDRSFTTSFNPFSKSKNKNFNKSHNDSFDRINKMLITRASRDDVQQYCFKEDVFGIPLESMRMAEDSHCPVFVCKLVNHLETTHDSIESIYLTTSCSSANTSQSILSDGSISISNGGGLSSSNLYEGVSRLDQLAKMIKRGKEDIYKHMDDMIDSPLLGQLLKLFLNKLPTPLLNDGLLCESLRSIINPSEQHYKLSFVRSIIFSQPQANWSVFKLLLQHFRNLAKIEFYPITSDDNNNNNDNSQPFEITNQIDLLFHRISTIFGPILFKINNNASVQQNDNSIEIFYYILIHFNEIFNDNDDSTQYIVKKGNQVLKNGTVSQIILKLLDIHYKDETLLETFNLTHHDYFLPTESYIALLVNRYTNLVSQHPTSITSSTGIAVNDISNEWRIKVRERILFIIRSLIEFKPMFWSNIHECFSSVELIQTFSDKFISKHTTTAEELKYFQYLLDLSTKIIDTGNAPNALSMSADSISVMNLHNGHAPAPSAFSPPQSPTGNSHQPMLGASRIQDMKKFNIIDMDATQIAIQITLIDEKSFRMIPVFELLKKKFTNPDLSPNYQNMVKLFNRWSAFVGSEILNHSTPGQRAAVIEKFIEIAIALKSLKNFHGCYSITQGIYHYSIKRLYLTWDKVSKKSMALFQELQNLFSTESNHKVYREMLHASSPPLIPYLGLYTKDLLVSEDSNPTFLIPKSPSSVIGEEKEQIINLEKMRSIYQVIKNWKLYRVNPYPMKTNPIQRDKIISRNILNDDELFEKSLLIEPRIRGQTTAALNPPLVNWSNTPLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPH6</accession>
    <name>A0A8J4UPH6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">COP9 signalosome complex subunit 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_009426</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CSN8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000705">
        <property type="protein sequence ID" value="KAF2069256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UPH6"/>
    <dbReference type="OrthoDB" id="5351233at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010387">
        <property type="term" value="P:COP9 signalosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000338">
        <property type="term" value="P:protein deneddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033205">
        <property type="entry name" value="COP9_CSN8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033464">
        <property type="entry name" value="CSN8_PSD8_EIF3K"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13339">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13339:SF0">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10075">
        <property type="entry name" value="CSN8_PSD8_EIF3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="8">
        <location>
            <begin position="10"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2069256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="192" mass="22174" checksum="E3E6A20127A0AA58" modified="2022-05-25" version="1">MSFKDIQDKINNKDFDGILKYCQDIECESSEKIASLYGAYLFCYLIKNDITNARFLWKRIPSQLRSNESLKSIWTLAKSIHQFDTKTIYTLLGSFGAEYQPFVVFFKESFQNRTFNLISNAYTSISLSDCINYLGISQEQTIELAKSKGWTFDESSKTFTPLAIPTTQSNLPQTDKQLQNLTNYVLFLEKSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPL3</accession>
    <name>A0A8J4UPL3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Porin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009289</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000672">
        <property type="protein sequence ID" value="KAF2069391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UPL3"/>
    <dbReference type="OrthoDB" id="7827681at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008308">
        <property type="term" value="F:voltage-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="entry name" value="Porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001925">
        <property type="entry name" value="Porin_Euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027246">
        <property type="entry name" value="Porin_Euk/Tom40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11743:SF70">
        <property type="entry name" value="GH26960P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11743">
        <property type="entry name" value="VOLTAGE-DEPENDENT ANION-SELECTIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01459">
        <property type="entry name" value="Porin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="25879" checksum="59BFBD7231A24F54" modified="2022-05-25" version="1" fragment="single">SIVATNEVNKAGKWVGTINPKLTLNPHLKTPGTASVSLNTDAFNKGEITFDSLFPGLKTVLTVDSKKIFSTEFQYKKDKFAVTLLGKNDKSVLSSVSFAINSMFSVGVQTEYNFAKSSVKGVNATLVAKPRSDILITLTDKYIDGLLSVSTLYKPSAKVLVGGDVTVDLKAPQASPAFNLGAQYFINDASSLKFKINEASKLGLAYLITVNNNTKASASWNINGKDFKQGNSFGLNLTVSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPT7</accession>
    <name>A0A8J4UPT7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008973</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000610">
        <property type="protein sequence ID" value="KAF2069706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UPT7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="144" mass="16380" checksum="0E8C757F9B38E764" modified="2022-05-25" version="1">MLLIILLLVCFFFIKFGLFNYIRNVSGFIVLAISLCILAVLTVITIYFQKKETKRLVNLVNELNTNFCFRNIRFIIHPKTHTKTIEQQLEIEINPQFNSNSNYCLILPPPYSSLNITPGSTPGSIVESTPLLNSSNNNNNDNIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQ57</accession>
    <name>A0A8J4UQ57_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008462</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000524">
        <property type="protein sequence ID" value="KAF2070216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQ57"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="415" mass="48370" checksum="13F0BB9E606FDB4A" modified="2022-05-25" version="1" fragment="single">MNANNGLKELVLPESFDRDIYGQLPSTLQRLTILNTEFDKTLQDLPESLISFECHSDNVILDLSGHKSIRELDVFGKTVITYPTNLESLYLRDDDQEELEFSFNQEQIKKLCLDYYLLSKLKLTNLKYYIAKYHNNKMKLPPSVEILECSKDFGFDHNYITPNVKEIHLQLTEASLYHLSKPNEQKIVKRIYNQLPMVDNFFKLWRNVVIKSKILESFYDLIRTDLRYNIEDETLVHGDRFKNYNIVVCNENELEHLLKFRPERVHIHYTCSKIEKQLSTLIPKSTKHLIIDSYYPKIDYPQWITHLTLKKWGDIEASQLPPSVTKLTLLNNPFGELEGKIPPTVTYLALYSLKANPGEGIPASVTHLTLIDRPINTIDHNGLPDTIKRIKFTKLSLDRDSTNYEISSFISSRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQX6</accession>
    <name>A0A8J4UQX6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carbohydrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007373</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000389">
        <property type="protein sequence ID" value="KAF2071311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQX6"/>
    <dbReference type="OrthoDB" id="18207at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035174336" description="Carbohydrate binding domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="17173" checksum="BCFE32EC12046513" modified="2022-05-25" version="1">MKFIILIIFVLVTLTNAQTSSSCSKLLSPHHLANKCIIVSIAGVNNYSLSQEGYKGYMEAVDGGFKNKGIIVNLQTYETYCYNGKRQPFSVHAAFNYDNVKIFLNGTISIQNTYSNMKCFDYFATFESNFYLYSLTLRALDTCYTLDYGEAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4URJ1</accession>
    <name>A0A8J4URJ1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006379</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000292">
        <property type="protein sequence ID" value="KAF2072301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4URJ1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="30252" checksum="D6474496CE0359DA" modified="2022-05-25" version="1">MTLETNTSDSETQHLLSQTNNSHINNNSNFQQSQLPFQQTQPSPTHISYQQQQQQQQPIFQQQYPHVEINPIPTNCSRFEIGVQYNNAIGTFNGFDPHTCDPILLNSMTQDEYREAVLSFNKVIVVHNWIIFIPIVLVGMAIMMVLVSTVSYFFIYPGLFLLVMVTLLLTVFLYRRKKRAINQVVADLNLKYQSRQIKFDVIFKTKKTKSMRKRNPICTIYIYIPIQQVDTYQQSYQTQQSQLYPQQFQPQQVPTYHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UUL7</accession>
    <name>A0A8J4UUL7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BP74 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003054</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000091">
        <property type="protein sequence ID" value="KAF2075631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UUL7"/>
    <dbReference type="OrthoDB" id="14772at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056422">
        <property type="entry name" value="BP74_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053344">
        <property type="entry name" value="cAMP-inducible_BP74-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35883:SF1">
        <property type="entry name" value="CALMODULIN-BINDING PROTEIN CAM-BP15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35883">
        <property type="entry name" value="CYCLIC AMP-INDUCIBLE PROTEIN BP74-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23621">
        <property type="entry name" value="BP74_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035253808" description="BP74 N-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="BP74 N-terminal" evidence="2">
        <location>
            <begin position="22"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="139" mass="15957" checksum="0665459BE46ECF12" modified="2022-05-25" version="1">MKNYTFVLLLLLLSLLCVCFAETAYFAVKPAHYDRDEFIIQLHDDRKISEARKILSGDTTGATHVMGRIRKVQKSYNPKYDYHLDPETISFFEMAIEVCDSSATYLNDHLDEACGAFLPGCFWCPWGSSLVRELKDKAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UV07</accession>
    <name>A0A8J4UV07_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_002588</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000072">
        <property type="protein sequence ID" value="KAF2076126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UV07"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035290260" evidence="3">
        <location>
            <begin position="23"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2076126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="229" mass="24522" checksum="D1EA4AFF2CC7A0DE" modified="2022-05-25" version="1">MKLFYIVLVFIAFSIAISKSYSIPHEIGSSSKLFISPHLQFDQASSEINGGDSGGSGSNGSLLPDSSSLPPGSSQGDSSDSLTSGGSSTIPISSSSNSQNSNSNSNSNSNSNSNNNNNNNNNSNSNNNNSNGNNGHHNSNGSKDKNDSKEKHDSKETSEEEHKKAMKRMSIIIGACFGVLVFILLVLFALYTLMKRRSANNIKNNFTYFKFGSNVDDAPLLSLEYDDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVP1</accession>
    <name>A0A8J4UVP1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">CYY_009814</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Required for proper chemotaxis and phagocytosis; proper spatiotemporal control of F-actin levels in chemotaxing cells. Negative regulator of the PI3K (phosphatidylinositol 3 kinase) pathway. Predominantly phosphorylates serines and threonines and tyrosines at a lower level.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. SH2 domain-containing protein kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000816">
        <property type="protein sequence ID" value="KAF2068865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVP1"/>
    <dbReference type="OrthoDB" id="339325at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000476">
        <property type="entry name" value="PAS domain-containing protein tyrosine kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000659">
        <property type="entry name" value="SH2-protein kinase domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF306">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE SHKC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="14" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="31"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="17">
        <location>
            <begin position="401"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF2068865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="511" mass="58003" checksum="278A8D99E73F5FC2" modified="2022-05-25" version="1">MDPQYSRDQFNNFQDFNSERSGPPEIRPEEITFEELIGTGSFGKVYKGRCRQKAVAVKLLHKQNYDAATLTAFRKEVHLMSKIYHPNICLFMGACTIPGKCVIVTELVPKGNLETLLHDQKMQLPLYLRMRMARDAALGINWLHESNPVFVHRDIKSSNLLVDENMRVKICDFGLSALKQKHKMLKDQSSAKGTPLYMAPEVMMFKEFNESSDVYSFGIVLWEILTRKEPFSHHRELEKFREAVCVKHERPIIPADCLESLRKLIEKCWDKDPQHRPSFKDIISSLDHIIIDAAISDLRGREFWKKNFITEQEVPSEAFIDALCNWAKAPTRNQCDKASLEYLNIKCLKAVLADSSKNEGSIVNIEKFGKILEFFGPMNETTAPGTTSIMDTIREVLSHKWFHGDLDTTEAASRLNGQPIGSFLIRFSSTNAGCFTISQVVGDNNSIKHQRVSRQGPKFIYQNILYNSLIEIVSKSAGGNNGDLPLNSNLGVPNYKFLALFCNVSSSSDTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXB2</accession>
    <name>A0A8J4UXB2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000273</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000005">
        <property type="protein sequence ID" value="KAF2078406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXB2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2078406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="193" mass="23223" checksum="CBE067E5D2681495" modified="2022-05-25" version="1">MQQVPQEEEEEQLYSRPSKKIKLSYVSESPNFSVHCFLNVSLIAPFFISPNGYIQNRVLMQEKLLECYSYLTFATPSLQEMYCFNLNVLRSIDPTFRMFLDVPYREGDEIKFQHVFLQRPLDFFYGYSHFKMELLKSCLNKHFLKLIEYILPFFNPQELQFVQENIKTHKLEWFGNNNTNPYLDFINKNDNDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXC2</accession>
    <name>A0A8J4UXC2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007955</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000453">
        <property type="protein sequence ID" value="KAF2070725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXC2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="718" mass="83479" checksum="8B0AFB278209394C" modified="2022-05-25" version="1">MNCRNKQQKLIFSHYDNLFPLIKKDFELFKILYSEYKYSSTRSIEDQDGSEQRIVFKSDFHRVVNTIQHIDAVKWMCNNGYGVELEVGDIVFGDESNPDVLEYLIQQGWYKVAYNSIICNHQKKNSVFTLPILEVFAKYTPTPITSEQVKEIIEQLAIGPIPMVFEILSPLFQDELCLDIPARTFACAPMAPSTLLIINYLYEKNLCKDITTLMVSDEFNTISKVSSFFASVLNIEVVNNGDQSIGKHIQKLIGHVPKFSKQTSEMIERNITLRTDLFSLPLEAIFALLDHGLQLSRHWFGRCIEKRSNHLFISLWNKLSSDHQLEITSNGRCILFYPNAKDRIRFHSLLHNWTGTNLEVLLFLIAQGMAGDELKRSIVYDFYRTMAKMYHHERDYKLIVELMSLGALECDTILDNCIKQRNISMFKYIYKHRVTDADNSYLLDSCIEQQDYQFLQVIESDNVKPNLIQSLNSILSFHRHEVRFIRELLKSSCTSYQTLIILLNTHIEANCFAVVKYLLTTFSYKSIKPFFGSLSKTTKPIIDLIYQNRDTIFSVAAPNASDWEHLLSLAIFQNNCALVEYFLHYKIVTSITISLKRLRFGLPSSQLLHLIRPLLSSDLHQEILDLPFISVNPTFHDIMYLIDHRVYESIDPYIDHIVSMCHQTNYYQTIAQTLEYISKNQHLFKQPVKFDNLSNLPLPQKLLTLFKDQSSRNNIFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXC4</accession>
    <name>A0A8J4UXC4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008383</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000511">
        <property type="protein sequence ID" value="KAF2070303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXC4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="443" mass="49747" checksum="024E77EAA89178B0" modified="2022-05-25" version="1">MATIKQEEIRVNHPDDLHVLKGLGSGQFKFMGYIGEMSEKARQLNQEMNGATQTPATPISISQPPQLITTKNVAAAFKNNDWNTIYKLQAQADKDLSPIYKKFEDDPSKSWVMVRDEATGMKYHVSYVNTGSYAWTFSSYVDYGSDPENSHVKFTCSCQYGTYSKNTNIMGIHRYFLGLVKFASELTVSGFLAHFVNRLLRKGINFLSKDLGSLLTDAAADAGLDALFTIGTYVMPIFVPCAVFVIIFIGISYLWKWLNKRFTIYININNWDKDNDWYIRNQTFSNAFNPGKDNAKSLQIKIPKVLPPGSKVWEKFWVPSDISSNLMHTLDTVVSYASLVYENDHHFAQGLSMALTVSLEKTNIGMSFAFQCPWSAKNAQYMEGTIQDPDQFLSKARSHWIESTATNKITLGNGTNVEATIDKLKGGDPNGEDTYCVTINIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXG5</accession>
    <name>A0A8J4UXG5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Pterin-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">CYY_008233</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(7,8-dihydropterin-6-yl)methyl diphosphate + 4-aminobenzoate = 7,8-dihydropteroate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19949"/>
            <dbReference type="ChEBI" id="CHEBI:17836"/>
            <dbReference type="ChEBI" id="CHEBI:17839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="EC" id="2.5.1.15"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>6-hydroxymethyl-7,8-dihydropterin + ATP = (7,8-dihydropterin-6-yl)methyl diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">In the C-terminal section; belongs to the DHPS family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">In the N-terminal section; belongs to the DHNA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000490">
        <property type="protein sequence ID" value="KAF2070453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXG5"/>
    <dbReference type="OrthoDB" id="615426at2759"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00155"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005740">
        <property type="term" value="C:mitochondrial envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003848">
        <property type="term" value="F:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004150">
        <property type="term" value="F:dihydroneopterin aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004156">
        <property type="term" value="F:dihydropteroate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00739">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00483">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.560">
        <property type="entry name" value="7,8-Dihydro-6-hydroxymethylpterin-pyrophosphokinase HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="entry name" value="Dihydropteroate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045031">
        <property type="entry name" value="DHP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006390">
        <property type="entry name" value="DHP_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006157">
        <property type="entry name" value="FolB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000550">
        <property type="entry name" value="Hppk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035907">
        <property type="entry name" value="Hppk_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01496">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00526">
        <property type="entry name" value="folB_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01498">
        <property type="entry name" value="folK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941">
        <property type="entry name" value="FOLATE SYNTHESIS PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941:SF1">
        <property type="entry name" value="FOLIC ACID SYNTHESIS PROTEIN FOL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02152">
        <property type="entry name" value="FolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01288">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00905">
        <property type="entry name" value="FolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55083">
        <property type="entry name" value="6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="Dihydropteroate synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00792">
        <property type="entry name" value="DHPS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00793">
        <property type="entry name" value="DHPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00794">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pterin-binding" evidence="17">
        <location>
            <begin position="336"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00009951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF2070453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="637" mass="71708" checksum="B91DC0F6DAE679E7" modified="2022-05-25" version="1">MNSNSNNSSQILNSDKIIIKDLHVQAVIGVNANERVIKQNIIITIKAFRDLTKCGSSDSVHDTISYSTLSKAIISYVESSHHYTLEALATGVAKICCLGFAIDRVKIVVQKPGAVKLAKWPGVQIERTLDYFKSNSFVEIPSSLNNSNNNNNTNNNNSNSQNNNKNNIVYLSIGSNLGDRYENLIECLKQLAKKCYVLSTSFIYETPAQYYLEQGPFFNIACKVSTDLLPMELLAFVKSIEKEMGRQETFRNGPRVIDIDIIYYNRMIMKTDTLEIPHEKMWERDFVLMPLNDIAPHFIHPSLHITTNQMKINLSSNSTKKMLRVGKVDWSLNEKTFIMGVLNVTSDSFYDGGKYNDEKRCLEQAKKLIESGADIIDIGAQSTYPGALQITPEQEIEFVVPLIKAIRKQFANVLLSIDTYNSSVAREAIKAGCNMINDVTGGFKDVQMLSVAKELRVPIILNHNQPTPQYLQQQQMVLDTTQQAPQNKATLKDIYFNNTLINNTDPSIINILNIFFKERVDTAMKMGLYRWQLILDPGLGFYKTYPQSIEILRRGKEIASLDFPILIGPSRKGFVGATIAKQDPLTDEIPKANSDRRLYGTVACCCIAAAWGVNIIRIHDVPEIRDSILISDSIYKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXH0</accession>
    <name>A0A8J4UXH0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histone H2A/H2B/H3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007764</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the histone H2B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000432">
        <property type="protein sequence ID" value="KAF2070920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXH0"/>
    <dbReference type="OrthoDB" id="9832711at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="196" mass="22190" checksum="948221A7562B64DE" modified="2022-05-25" version="1">MYNRGKLGKRKFTRIGYHSKHPREVQFLKKLIKECQFLKKKKLPIRVTASHVLASMIRYVAKEIINTAIELTAIDCCTSKQKQQSTRKPIISDRTIQTAVTLVLSGELSKYAVLFGTKAVQEKKKQTISVSYVQAQIKASCAAVSFTKDACIYLVGVLEYIFLELLDITVVVANYRKIPVCSYIVPYGLNNHVIMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UY05</accession>
    <name>A0A8J4UY05_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA/RNA-binding protein Alba-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_006852</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000334">
        <property type="protein sequence ID" value="KAF2071835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UY05"/>
    <dbReference type="OrthoDB" id="416729at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014612">
        <property type="entry name" value="Pop7/Rpp20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15314">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15314:SF1">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="AlbA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DNA/RNA-binding protein Alba-like" evidence="5">
        <location>
            <begin position="58"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="158"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="158"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="201"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2071835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="218" mass="24088" checksum="C7F3EFC91AEB5F39" modified="2022-05-25" version="1">MTTDNNHDLELHDNDSVSSDDDDELLMDEKDKEFQMINDPTLYTYVRRVVQRPQTNKNDIYISTNGKYIYYVKRAKQLLFTQGLKEITIHGLGSAIGLAVKLSLYLERNIGGLVLSPTTSTEEIIDQYDPLVNDLEPVLKIRHCSSIHIKILKNSVSTGTATTATTTPTTTTNTQESVTEESSVNPLSIYPTSYKSRGGRNNRGGGRGGRGGGNSSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZX7</accession>
    <name>A0A8J4UZX7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pesticidal crystal protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000982">
        <property type="protein sequence ID" value="KAF2068447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZX7"/>
    <dbReference type="OrthoDB" id="22869at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.10">
        <property type="entry name" value="Pesticidal crystal protein, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036716">
        <property type="entry name" value="Pest_crys_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38082">
        <property type="entry name" value="ENDOTOXIN_N DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38082:SF2">
        <property type="entry name" value="PESTICIDAL CRYSTAL PROTEIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56849">
        <property type="entry name" value="delta-Endotoxin (insectocide), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="519"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="568" mass="64728" checksum="7C96E0F9BC1DC6ED" modified="2022-05-25" version="1">MTKGLGSKLKSTSDAFLSSTKENVKEKYDGVVDDLKKDPFAIIDIEKIGEIFNDPLGFVGDLITDKEFIGQMIGAAIECIPVVGPILSSVFSIFWPQDPKMKAVTVKELDERLTKFKKEMVKIIADKIDASEKETWKELCKTFFEGYIFKISQMKRSVLILKREIKDRNGVIADVPEATKERIRVTFLSLNESANNISNFCKKQKISNQVIEIYMQTILLQCVSTIQLCSFWYQIDLDKYYILGMQKEEEGDEEVKSIKQEYHDLLVECLKKITPAVYKNIVDSKPKLNKYYKNTAGVMFNTNPWIYPIPLVIAPLNVPRVATQLHLRSSTPTINVPLERKTPFIYRIDGANMLPDQMKEGHNGSRPIPEIDPLTKCYGTFIDLSNSINISLPERKNVTIRIFGIYTKSISNLSLGAKLKIKQECVQESIFCLPPSLKRGNNFDGKVENSLLVAESEGELYLEANGAKSGFTYLKRYRNVNTICLNIGQESTGNFYHRGCIKFLELIISDVSAKDLQDIDNVEILIQEKKDQIKKLHKIKNFTEDHVKEFEVLNAELRKLKARLNRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V057</accession>
    <name>A0A8J4V057_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Oxidoreductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003528</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000111">
        <property type="protein sequence ID" value="KAF2075180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V057"/>
    <dbReference type="OrthoDB" id="2129491at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051450">
        <property type="entry name" value="Gfo/Idh/MocA_Oxidoreductases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377">
        <property type="entry name" value="BILIVERDIN REDUCTASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377:SF2">
        <property type="entry name" value="BINDING ROSSMANN FOLD OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G00560)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="1">
        <location>
            <begin position="21"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase C-terminal" evidence="2">
        <location>
            <begin position="157"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="433" mass="49576" checksum="75A99E627BF3BE05" modified="2022-05-25" version="1">MSNNNNNPLEDESKFDTEKPVRIIIVGCGNRGYVYSQYAIERPNRLQVVAVADPIQFRRENVAKPFNLDSQYIYKDWRDILKSKEKIADSVLIATPDTEHCEPAIAFAKKGYHLLVEKPLAITKSDCENIVKAVKENKVILAVCHVLRYSPYNLKIKELIEKNYIGKLLNVQHLEPVGYYHNAHSYVRGNWSQEKTSTFMLMAKSCHDLDLMHFFINKKCKKVSSFGSLSYFKKENKPKEAGNSKICFECPIINNCPYSAKTIYIDHFPWKKNTVVPTKEPTIEDVKEALLNGSPYGRCVYECPNDVVDQQVVILEYEQGITATFSMVSTTADMCIRKTRIFGSHGELECNGDTIIWSDFIKDDKEVFRPNVIQNTRMVNHSSSDYYLIRSFVYAVATNDPSQILSGPDDTLESHLQVFKAEESRLKNKVLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0E5</accession>
    <name>A0A8J4V0E5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004329</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000151">
        <property type="protein sequence ID" value="KAF2074353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0E5"/>
    <dbReference type="OrthoDB" id="15362at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="139" mass="15815" checksum="5BB3E2F7AA5E8F8F" modified="2022-05-25" version="1">MSTETQINSPTNSQGYNWGIWKADWKQWWGSTLGWESLEKQGLEERKKLTPNPRGTPLKKGNSSSEDVSIGVHGGKIDHPATVASFLPEWKYPAFERNKYDDELLRQKIRETPKEQVIPTKEYVTGHPKGQYVTGTPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0G6</accession>
    <name>A0A8J4V0G6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BP74 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003053</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000091">
        <property type="protein sequence ID" value="KAF2075630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0G6"/>
    <dbReference type="OrthoDB" id="14772at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056422">
        <property type="entry name" value="BP74_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053344">
        <property type="entry name" value="cAMP-inducible_BP74-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35883:SF1">
        <property type="entry name" value="CALMODULIN-BINDING PROTEIN CAM-BP15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35883">
        <property type="entry name" value="CYCLIC AMP-INDUCIBLE PROTEIN BP74-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23621">
        <property type="entry name" value="BP74_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035171365" description="BP74 N-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="BP74 N-terminal" evidence="2">
        <location>
            <begin position="19"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="133" mass="15128" checksum="3BC34FC9796DA901" modified="2022-05-25" version="1">MKSIILFLLFLFVALSVAETAYFEMKPAHYDQAPFIIQLHDDRKIAQARKILSGDETNETHVLGRIKKITKPYNPKYDYHLDPDTISFFAVAIEVCDSSAAYLNDHLDEACGAFLPGCFWCPWGSKLVKEINI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0R3</accession>
    <name>A0A8J4V0R3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">4-hydroxybenzoate polyprenyltransferase, mitochondrial</fullName>
            <shortName evidence="9">4-HB polyprenyltransferase</shortName>
            <ecNumber evidence="9">2.5.1.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Para-hydroxybenzoate--polyprenyltransferase</fullName>
            <shortName evidence="9">PHB:PPT</shortName>
            <shortName evidence="9">PHB:polyprenyltransferase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">coq2</name>
        <name evidence="10" type="ORF">CYY_009126</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the prenylation of para-hydroxybenzoate (PHB) with an all-trans polyprenyl group. Mediates the second step in the final reaction sequence of coenzyme Q (CoQ) biosynthesis, which is the condensation of the polyisoprenoid side chain with PHB, generating the first membrane-bound Q intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>an all-trans-polyprenyl diphosphate + 4-hydroxybenzoate = a 4-hydroxy-3-(all-trans-polyprenyl)benzoate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:44504"/>
            <dbReference type="Rhea" id="RHEA-COMP:9514"/>
            <dbReference type="Rhea" id="RHEA-COMP:9564"/>
            <dbReference type="ChEBI" id="CHEBI:17879"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58914"/>
            <dbReference type="ChEBI" id="CHEBI:78396"/>
            <dbReference type="EC" id="2.5.1.39"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="9">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Secondary metabolite biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Mitochondrion inner membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
            <orientation evidence="9">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the UbiA prenyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.39" evidence="9"/>
    <dbReference type="EMBL" id="AJWJ01000641">
        <property type="protein sequence ID" value="KAF2069557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0R3"/>
    <dbReference type="OrthoDB" id="18170at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008412">
        <property type="term" value="F:4-hydroxybenzoate polyprenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13959">
        <property type="entry name" value="PT_UbiA_COQ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.140:FF:000008">
        <property type="entry name" value="4-hydroxybenzoate octaprenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01635">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006370">
        <property type="entry name" value="HB_polyprenyltransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039653">
        <property type="entry name" value="Prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030470">
        <property type="entry name" value="UbiA_prenylTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01474">
        <property type="entry name" value="ubiA_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048:SF28">
        <property type="entry name" value="4-HYDROXYBENZOATE POLYPRENYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048">
        <property type="entry name" value="PRENYLTRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00943">
        <property type="entry name" value="UBIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="204"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="275"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2069557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="359" mass="40451" checksum="649DD274CD93A46E" modified="2022-05-25" version="1">MYFGKFISPIFQKGLYFKPNNKISVFGQPNFYIGNRKLCSFRSTKNLKTSLILSKQDIKESFVKKFPVSARPYVQLSRIDRPIGTWLFLIPSYWGLFLASPTPDLKLLSVFAVGSLVVRSAVCIINDMADYKFDKQVERTKTRPLASGQVTHKKAFVFLGAHFASLYAMTLPILNNQTILLCLSSLPLAVLYPFMKRFSYYPQAVLGLFINWGVLAGYYAIAGPIGLTAVIPLYISSFLWTIVYDTVYAHQDKNDDRMIGVKSTALKFAENTKPILSVLSVATIGGMALTGSLLSMPLVYYLGTGVCATHLFYKLYRVNLDDADDCSDFFKSSRDFGLIYLVTIFLSHLYQDDKKKEIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1I1</accession>
    <name>A0A8J4V1I1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin-related 2 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001609</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000039">
        <property type="protein sequence ID" value="KAF2077100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1I1"/>
    <dbReference type="OrthoDB" id="17592at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20558">
        <property type="entry name" value="CYCLIN_ScPCL7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF106">
        <property type="entry name" value="CYCLIN-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="249" mass="28278" checksum="F02893C00753B35B" modified="2022-05-25" version="1">MGGPMTLLESLKTILPTKEYNEKDKDSLLLEIITLVICKMVKAGDDIGRNTHEQFMPPNKKLPAISIKDYMSRLMKYSPCSKECFVASLVYIDRLSIECGMLINSYNIHRILITTLLISTKYLDDIFYNNEFYSQVGGVSLKEMNLLELDFLKMLNFSAFCPISTFNEYQREMETSKDRFLNFFNPSTLVQKISLSSPLNSSPMSSPRRSQSPSTNGSNFFQRRGSCDYDPTRGQGSSKDSTFQTNCAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1K7</accession>
    <name>A0A8J4V1K7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cAMP-dependent protein kinase regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_001511</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cAMP-dependent kinase regulatory chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000036">
        <property type="protein sequence ID" value="KAF2077190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1K7"/>
    <dbReference type="OrthoDB" id="417078at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007154">
        <property type="term" value="P:cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045595">
        <property type="term" value="P:regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023052">
        <property type="term" value="P:signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000120">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000006">
        <property type="entry name" value="cAMP-dependent protein kinase type I-alpha regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012198">
        <property type="entry name" value="cAMP_dep_PK_reg_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000548">
        <property type="entry name" value="PK_regulatory"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00103">
        <property type="entry name" value="CAMPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0114">cAMP</keyword>
    <keyword evidence="4 8" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="6 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="10">
        <location>
            <begin position="85"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="10">
        <location>
            <begin position="208"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000548-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2077190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="343" mass="39148" checksum="A1045C399E65EE9B" modified="2022-05-25" version="1">MTNISYNQKATEQVEDPINDQQQQDQLQQQLLQQQQQQNMIGTRKRRGGISSETLSEEPSIAIPTVPKTRETQDRLDQALEKNIMFNHLEEEERKGLFSAMVEVHYKSGDVIIRQGDEGDNFYVVDSGVCDIFVSKNGATPVLVMEVFEGGSFGELALINFSPRAATVIAKTDVRLWALNRITYRKILMDQTMTKRKMYEEFLEKVSILKDIDRYERISLADALEPCNFPAGDVIVKQGEPGDKFYIIVEGEVLVSHQTDLNDPSSSHEVIRLHSGDYFGEIALLLDQPRAASVTAITNTKCVEMDRQRFIRLLGPCENILRRNMERYNQYMTSNSPKLTHHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1Y8</accession>
    <name>A0A8J4V1Y8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000022">
        <property type="protein sequence ID" value="KAF2077685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="441660at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053331">
        <property type="entry name" value="EGF-like_comC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24032">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035198977" description="C-type lectin domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1264"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="35"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1319" mass="145499" checksum="116DC8FE5E6EA4A6" modified="2022-05-25" version="1">MYYQVIVVIVCLVFIIVNVTADDDNQLIVSSVNTYNNHLYRLYKAPKNIGQQDAIQACLSRQGYLITITDRYEWEFMVALVQSSAVGNVKMWVSGTDVSETGNWVFSSGPESGALIYNYFTDICYSYCNWTSGEPNLQFTEHYLAYDSAKMSVGNFNALMYFMCEFDNGNPLLPALSSLGGEIPVNFKGVALANLAFTMTNMVTNQNIPVVVNTDSSILSIGPNVGLYNLTIVNKATGELYSNGLWQYSPPALTSLALALDGVTLSITGSGYGVTASRLSVRTASLGCKITSLSQTMIKCTLDRALSPTETLLPVIVSVDNGSVMTTSFRVAVLNPASSTYYSSISTISTFPDVYTYYIFNNGDGGMRSEGQNGYLAILSNDNRDFFKRTFNAYPAWCLWQPVQYNQTAKRFYSMYGPKAGSFVSVYGEDTLQKDPTWTSKTRFYLDAIKSNLVAVDESVKYRCSLLIQLGGYSPEFTDTSFTVPTKSSTINVGVKHIGMVNDASTFTASSGTVSNAKRNIDSQSVDLTLPDGYQPFTIKGSINNIAFTNSLTIQYQSPSIQSISANANTLTINGSNFYKDAKLIAFTSGSSPCLNVKITVAHQQFTCTLSASTLFAPKYNTLSVGSKTVYYVFGKNYYDLTDAPIVLTSYKIEGYNLTVTGYNLAKASVARFPPGNVLIPSSSYNRSTDISLGAYQNTFVLPPKTKSGLAAFGPNLNSDIVYNQTILLPPVINAIEDMTNQANGTVFSMNGGLMVRWIDNTDTPTPLFYRVGKEDIKPLLCDIDTTNTYSVFLYQNCFVEIGTSNASMISILYAKNWTSKDYTQVYVFYRYNPIIVQSTSLVYQIPGLVNITVKNLPSNLTNVVITIGGSECRTPSITQDDKITCFFNADVPLMALGQSLPVTINNIATNVTITNNVFLYMPRVNCPNPCNNGTCDYSAGICLCAPGFFSYNCLSTVPPIPTRPELDSEFGSITLPGGKQINFTTSVSHIRERNMLDETVKILPMNKIMWVDNLTIPSIPDTGVPATYIQKGIFYQETVIVQISIKIYENDTIIDFAGEKINISANSVKYEISVANWEFNSTTNYLQVIFSTNSSQSITNQCDDSRPTDAISNSNNTSTELQPQLLQLTQANANDVVSSLTWFSLDANGNRLEARFSNRIIVDSRVQDSIVQIIDTTDPEIYPQQQPPPTQTQQPNNIDNINTVSPQPSINNNYQHKLYTSISIPYFKTRAVLDPNFRVLIERNDGGKACKSHANKGLSESKWRMTVIIVTMSVVGVFVTIASVIFLIKRSRFKRATNSLKRKLQRAASSNNIEYLDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1Z9</accession>
    <name>A0A8J4V1Z9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Saposin B-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_007717</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000427">
        <property type="protein sequence ID" value="KAF2070967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1Z9"/>
    <dbReference type="OrthoDB" id="69496at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.225.10">
        <property type="entry name" value="Saposin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008138">
        <property type="entry name" value="SapB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008373">
        <property type="entry name" value="Saposin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011001">
        <property type="entry name" value="Saposin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008139">
        <property type="entry name" value="SaposinB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051428">
        <property type="entry name" value="Sphingo_Act-Surfact_Prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11480:SF3">
        <property type="entry name" value="BCDNA.GH08312"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11480">
        <property type="entry name" value="SAPOSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03489">
        <property type="entry name" value="SapB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01797">
        <property type="entry name" value="SAPOSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00741">
        <property type="entry name" value="SapB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47862">
        <property type="entry name" value="Saposin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50015">
        <property type="entry name" value="SAP_B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035264259" description="Saposin B-type domain-containing protein" evidence="3">
        <location>
            <begin position="19"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="4">
        <location>
            <begin position="28"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="4">
        <location>
            <begin position="123"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2070967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="203" mass="22078" checksum="C3A99E966D5032C5" modified="2022-05-25" version="1">MLKSLLVVILFLAVFSNAARVPPKEGDITTACEICELGVRFIIEVAQKNSSITYVTEELQHLCYIIPRGLQGACQEVISQYTPDLIRLIITSDNPEEVCSNQLDICSSSSSSDSDNVENVGDDATTCRICTFILNDAIAIMKAGGSRAEHLMGALNSDCSRYMPDPANTITCQYIVNTSMKQLLAYNKKGLDTVAACGQCLYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2D0</accession>
    <name>A0A8J4V2D0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">CYY_007281</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000379">
        <property type="protein sequence ID" value="KAF2071402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2D0"/>
    <dbReference type="OrthoDB" id="20451at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031090">
        <property type="term" value="C:organelle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20336">
        <property type="entry name" value="Rcat_RBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16773">
        <property type="entry name" value="RING-HC_RBR_TRIAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000051">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="359"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="363"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="884"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="792"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="885"/>
            <end position="899"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF2071402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="986" mass="112903" checksum="82004DAFB8BF75D4" modified="2022-05-25" version="1">MASTSVSHNHNNNKSTPQSIYVEYEVPDTRKRFTVSFIKDDKVHETLCVVIPNKFTWSGFIHLLLTKFKLEPNLKNKIRVKTNDGRRINDLTNTYNNQLIQFSLSNLYEEEQEDIQLDAQGLPSLKSSFNNSTTDKDSVKRLPFLEPGQNVESQFIKDAIKLLSTYKDHVIIGESYPYKVKVLLPLGGNSSLYFVEQPYPEEGEQENGNYKFNTIIHCNDIETFTKIKSVIDLKTDKDSIVSLASKVVNLSPKKKSNEYKGSVSEPTRSLFPNDFKVHFNISPNDFLSDHYSLTNLMSKKFDLPFSLARDFLFQVKWLFKKTRGFEKANFDEKYPTHTKRDVSQYNFNSLNLNLQDGDDVFDCPICYCDCGIQDTTELLCGHRFCNDCLQSYFISSIEDGNGAAVSIACPSVGCSNKFVDEVTIETLLPAKNITRLQENYVNDLMFFTRSKKCTCGYIYKKPYDTSCFIPFYECKCGANICLDCGEEDIHWPLSCKDARTDEDLQSYDWIAKNTTICPSCQFPVEKWTGCNHMTCSRCRHEFCYLCGNDYRGHMQCPPGRTTTYKYQKFFKALRGREMNPDVIQSFIEQDTKPAISQALKSIHNNMIFLSMRRKNATKTFIMSFEALFMTLSEYAYSKGGSITQDSITFLQRCTRKFMRETRKPPSFYQVLIDNSPVVKSLSANICLNGSASVVVSNVFVNTQFSEFKNEIVSALKKKNIEGYDVKNIRILNRYGGQIRSSLEIFDKEKLYIIKDSEEKFIAPFDDDSDEENESDQGELVTVTFGEMTKTFNELKQKKHAKLDNHLKKLEKTLKEKEAFKDHFKQLLLAPSTIAQDVVVDNKVDGDDTATTAADTTATTDDDGDEEILLSQFIKEFVKINKSDSDTATSESDSGNGSSSITDKEEQNLKITERILEIFKFENLEYDYVYQIVSISPDLNSACDIIYSELPPDEINSDDDHDEFYDTIEDRISGSVKRCGKSFYNQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2M7</accession>
    <name>A0A8J4V2M7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006962</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000346">
        <property type="protein sequence ID" value="KAF2071717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2M7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="151" mass="18622" checksum="60ED0ADEC3BDD455" modified="2022-05-25" version="1" fragment="single">QLEKEEESKSKDLSSFNRNLLNNIYNSKDEQISKSASTSSSTSSTSNRNNNNRDRDRDRYPNNNNSNNYYKRNDNRDRERDREYERDREREEKERKLEIERLKKEKEEKEEKEEKEKKKYERRNDSKSIQLARERYLLRKLKREEALTSFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6L4</accession>
    <name>A0A8J4V6L4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002750</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000079">
        <property type="protein sequence ID" value="KAF2075947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6L4"/>
    <dbReference type="OrthoDB" id="285802at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044772">
        <property type="term" value="P:mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20529">
        <property type="entry name" value="CYCLIN_CCNJ-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046965">
        <property type="entry name" value="Cyclin_A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20916">
        <property type="entry name" value="CYSTEINE AND GLYCINE-RICH PROTEIN 2 BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20916:SF18">
        <property type="entry name" value="IPT_TIG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001771">
        <property type="entry name" value="Cyclin_A_B_D_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 4" id="KW-0195">Cyclin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="203"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="314"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="106"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="433"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="116"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="128"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="438"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="468" mass="53909" checksum="1A8133A98C9BD248" modified="2022-05-25" version="1">MESNINNNNNNNNSRNTINNNSINLSTTTTTTTTSTSTSTANTPNPNINILLNSSQIIQSDKVEQNAVALRLRKNNNTNKKIPKVKLKIDQLLHIFKNLRKLPNQQDGQEIKCDQNKTNTDSQLIGLSPDLNTSSNTDNNSKNSQEQRSLLIAAVWEREFDLVAENKDHYDTLVVREKYYTRMTNLESLNFPNLTSTRLTVLTYIRKIGICQCVTRHTIETTFNMYEAYLDFKPYTPREQFQLIALTCFYIACTLFESSEKVPTFSDLNFATESNYTINDFPAMEIDIAKTLKWNLHFISKSQYIDYLEPILFHELDKESVKNNPNNITKHKFNKLLELIMTLFEYDLNSRRIVPSLAACACIAFTRKFLGIEPVWKLSLIMATGYHLEDFQDILSYLISTFTKQVLYLISTDITFHQEENPIVAEEEKPDQVPDVLNNNNNNNNNNNNNNNDFDLDPNVTVPEELYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6Z4</accession>
    <name>A0A8J4V6Z4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ER membrane protein complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_005307</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the EMC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000207">
        <property type="protein sequence ID" value="KAF2073394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6Z4"/>
    <dbReference type="OrthoDB" id="124397at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039856">
        <property type="entry name" value="EMC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055217">
        <property type="entry name" value="TPR_EMC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12760">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22890">
        <property type="entry name" value="TPR_EMC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="100"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="EMC2 TPR-like" evidence="5">
        <location>
            <begin position="102"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2073394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="332" mass="38707" checksum="BDBEA75CB80F2292" modified="2022-05-25" version="1">MTTELIEYQSYHDQLHKSRSFNWVLVRDLLRFLRKSKFRKSLIVSQYGYKLVDQYFSKLDPQEGWDLLEQVIVCCLDCNQIEKAQTLLNRLVAKFTTNSVRVKRLIGMLYECQGLIQEAINVYQTILQEYPSDSMSMKRQISIYKSQGIHNRAIQLLNTYLQIYMCDFEAWLELASIHINLLSYKSALFCYEEIILNAPINYLFYVKYAEISYSLGGADNFILALKYYTHSLELNSPLDYPEQDHPPTNLPSIYGIIMSIYSFCESSHGGVNKLKDSQVNLMEWAQKQLLDITNQYTPDKKDIVSAFIKLTDIPKKSTTPKDVEQQQPTIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7W7</accession>
    <name>A0A8J4V7W7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ribose-phosphate diphosphokinase</fullName>
            <ecNumber evidence="4">2.7.6.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_004295</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000149">
        <property type="protein sequence ID" value="KAF2074399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7W7"/>
    <dbReference type="OrthoDB" id="413572at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000001">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002320">
        <property type="entry name" value="PRK01259.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF32">
        <property type="entry name" value="RIBOSE-PHOSPHATE PYROPHOSPHOKINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="13">
        <location>
            <begin position="6"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2074399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="325" mass="35615" checksum="20493BCB4F4A454D" modified="2022-05-25" version="1">MSSHKLVILHGNAHPELAKEIALNLNSTCGNALVSKFANSETHVVINESVRDVDLYIVQPTCNPSVNDYLMELLVMVDGAKRASTHRITAVVPFFGYARQSKKDKSRAPITCKLVANMMEVSGIDRVITIDLHSSQIQGFFNIPVENVYSEPLFVKYIKKKIKPQGDIVIVSPGVGGVKRAKAISDKLESELAIIHRANKEFLLSSSSSFSFDIDTMLVGEVNNKIAIIIDDIADTCTTLRLASNALMKKGATKIYALVTHGVFSNQAIEIINDSPICELVITDSIPNNHNKEKCPKLKIIPIASVLAETIRRTHHGESVTSISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8B7</accession>
    <name>A0A8J4V8B7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">IPT/TIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_001044</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000023">
        <property type="protein sequence ID" value="KAF2077657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8B7"/>
    <dbReference type="OrthoDB" id="20492at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052014">
        <property type="entry name" value="Dictyostelium_Tiger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31341">
        <property type="entry name" value="IPT/TIG DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="749"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="5">
        <location>
            <begin position="611"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="679"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="703"/>
            <end position="734"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2077657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="799" mass="87765" checksum="F661F01E64A4697D" modified="2022-05-25" version="1">MLDYSKQTKVDGNTGYLSSFDNEAEFIFIKQYYPLTFPWYISLGVKVATLEPPDYYYTAGPRNGKNDNNFYNSGLGINPEWMREESYVTMNFEDRAIITRTIEDSGLDIYGGLIEFGGYLPTVRNYQTPYLINTDGGTIFVQLNNFGTSLSTTLVQTTNLLSFSISYYKGGISMTISHVDLVAQKIPIKIIIDKVEATPSIYYDRSHPSITNIKPSIDTAGGVITITGESLFNQESAIAVKGIVCNDIQFTTPHKQFTCSIGPGAGMKSISIDVGGKVSNTFDYNYQYPTITSIPPIPTSGGIVTIQGTNFGIDKNDLSIDLCTNFEIITPHTTITCEFNQGQGDLSVILTIGTYSTPPFNTRYQYPTISNIVQTNGDIDIIGDNFGWDQSKSIIKMDNDLVIGSSCTLVSNTNIKCNNLASVYGPFSISNDKGVNFGTTYKYDLKPYITSTFEGVCSNPQEKLTLKILYMVSKSSTFKIKYGGQYFQVNVLDSLTSEFIVPDGSGVVPLQFEIDNTRLSNEISISYCPTVIKSIDFNIRTRKYEITGTGFSSNSNKGLITSTNGLSKLNLQVDQFNSTFISIGLPMDAKSGLVMIDTSYQLSNKVWINITPEIESASNPLEQGSPITITGKYFKTVDIDGNNIGLEFSLNGNKLPCQAGSNETSVVCQVPPGSGKITINAKSSSAKNGESDSTDLTLTYIKSPNNNSNNSDSNNNNNNNNNSHSDGTNNNSSTSNINNSNYNLWKLKAPLIVVSCFVGVGLSVGFIIFLRSKAASDFLNRFRSRRSRMHRNEDSFELM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8T5</accession>
    <name>A0A8J4V8T5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000593</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000011">
        <property type="protein sequence ID" value="KAF2078122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8T5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2078122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="113" mass="13807" checksum="8FFADD8A70D7C9A3" modified="2022-05-25" version="1">MDRLYRSIFRNVYLSKQIFTHVSVIQQDDNSLKYDDIIEVEWMAKYGHIGLLSDKLHRGAALFYERPWDFKVFKALLKKDFEVFKIFYQRFYKYNTYIKDDRNDFHDSVVLGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-03-15" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1N7TL88</accession>
    <name>A0A1N7TL88_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glutamine synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">glnA3</name>
        <name evidence="6" type="ORF">CYY_000500</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>A core phylogeny of Dictyostelia derived from 50 functionally divergent proteins retrieved from five existing and six newly sequenced genomes.</title>
            <authorList>
                <person name="Singh R."/>
                <person name="Schilde C."/>
                <person name="Gezzard T."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EMBL" id="KX274476">
        <property type="protein sequence ID" value="ANT82576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AJWJ01000009">
        <property type="protein sequence ID" value="KAF2078210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1N7TL88"/>
    <dbReference type="OrthoDB" id="415358at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040577">
        <property type="entry name" value="Gln-synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052725">
        <property type="entry name" value="GS_Type-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022147">
        <property type="entry name" value="GSIII_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42974">
        <property type="entry name" value="GLUTAMINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42974:SF1">
        <property type="entry name" value="TYPE-3 GLUTAMINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18318">
        <property type="entry name" value="Gln-synt_C-ter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12437">
        <property type="entry name" value="GSIII_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51986">
        <property type="entry name" value="GS_BETA_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS beta-grasp" evidence="3">
        <location>
            <begin position="89"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="GS catalytic" evidence="4">
        <location>
            <begin position="188"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ANT82576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2078210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="734" mass="82341" checksum="1F6759A21F5E8719" modified="2017-03-15" version="1">MASYRRADFIQYIDSREPVSTIDQDEIAENLTNIYDSDTYGLNAMRQTLPSHCYKKIREVIASGQPLDAQIADMVANGMKEWAIKKGATHYTHWFLPLNGLAAEKHDSFINIMPGDDKVLLEFSGMQLIKGEPDASSFPSGGLRSTWEARGYTVWDATSPAFIRREKNGAVLCIPTAFCSWTGEALDQKTPLLRSMEYVSEESLLTLQNIFNEKYKKVFPTLGIEQEFFLIDRKFYLSRPDLVNCGRTLIGAKPPKGQEMEDHYFGTMNSRIISCIQEVEWKMWKLGMPLKTRHNEVAPGQYEVAPIFERANLAADHNMVLMDVLKASATNHGLVCLFHEKPFANVNGSGKHNNWSLSTDTGLNLLDPGSTPSQNARFILFLAAVIRAVDVHSDLLRASVAVPGNDQRLGGNEAPPSIISIYLGQELDNLVSNIVNSTNNQFSADADMNLGVIGFPPLPKDSTDRNRTSPFAFTGNKFEFRAVGSSQVVNFPCIVLNTTVAESIKFIREEILKEMQTNSRQNAFNKVIKDVLSVHSRVIFNGDGYSQEWRDMAKSRGLLSLKTTEALSLMSSEKNIKLFSADGILSPVELACRQEVLFEIYAKSMTIEAKTLYDLIATLIVPACFKHQKNVADSLNSIMPFIQGQKSFCQPTAQLQHLSDLVDNINRLIEQNQKLMGLIKQCSEIKSEHDRAFFLQQNVSAAMLDVRKISDELETIIDDTIWPIPKYSEILFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJ77</accession>
    <name>A0A8J4PJ77_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010397</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001084">
        <property type="protein sequence ID" value="KAF2068277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJ77"/>
    <dbReference type="OrthoDB" id="6130531at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053331">
        <property type="entry name" value="EGF-like_comC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24032">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EGF-like" evidence="1">
        <location>
            <begin position="778"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="1">
        <location>
            <begin position="909"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="924"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="924" mass="100829" checksum="B1326B286EC48FE0" modified="2022-05-25" version="1" fragment="single">NECSKKETTTQSTNSDDTIRNIKITMNGETLIGTFSDRMILDTRPTYNKVNKLTTDQINKYQLDTTTATIYTSILTSYFKNSVIVDPNFGVLVSSAPDECSISKVNSVPVISNITPPYSLNSLSSYITIHGTGYDAGLKIYTDGVECPNSDSTPTPTQHYCLITTLSTNNQWSVQAKNGDGMVSNIYTQFTITFITLSQTNENVYINGDCSGLPDEYRSKIKASSTNKQISVPVKWSQANLTFKLTKEFFSAADNFLIIDSISNSIIANERFYYSPVITRLYYGESQIFITGFVFTNATGSGMLLNDVICPTIIHSETEASCTPLNRFFITTSGPFTVSMVRVSTKSKTTLSDSLLTITAFQNIVVNNQVMSTLSIQNLDYNKSYKIGGISPTPIPVDSFDSTSLQFTYPYDAQCGYAFVILASDSSRVSNSLFVCPTPIINSIIPPVNGGNVYMNGYFLNNPMVDSDPPSSALKIESYISKNEYISMWETTSFDFTYSSSNSTYSVNYIIPRGTLDKVIIKTLAASVSTQFLVVYNPIVKSCTTLSYKVPGLVTISGLNFLSTSIPFNVAVRIGGSRCTNVLVNPDGSQITCQFNADVNVVDLQPLPVSVVVDDIYQGSSNVFSYVFICLIGGNGKECSGNGVCLNNDCVCDKGWEENDCSEIYPLVNSVSSTKYRVPAYVTILGKNFVNKNLQVNIANSICSDVAVSQDLTTITCFYQSNVVVNNVKDYLAVTVSIDSQFTTSSNLFPQPNQECPFDSDHKLCYGNGICDSFTGKCSCYKGWESSGCSIVVPLINSITSTKYKVPGTITIIGNNFVNLNLVVTIGGSFCGNIVVSQDLTSITCLFQSDISVTNINEGLDVFVGINSTFTTTSSKLFLYTKPDQQCPIGSNGQICSGHGNCNQQQFTCDCESGWESSDCSFQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKP9</accession>
    <name>A0A8J4PKP9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010088</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000918">
        <property type="protein sequence ID" value="KAF2068585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKP9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="113" mass="13068" checksum="441ADE189D9084FB" modified="2022-05-25" version="1">MNEKTKSRTEKFFITAYVFSFSTIWVPLITIIETYVFVRGMAMFAIITLYEIELIFLVYPVNHHLIIKFFNSAYNLPTPESTTTPQNISSPSNATSEKIEELETIETNQHVQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLC8</accession>
    <name>A0A8J4PLC8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_009635</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000762">
        <property type="protein sequence ID" value="KAF2069048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLC8"/>
    <dbReference type="OrthoDB" id="5575at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003287">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein 200 kDa helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041094">
        <property type="entry name" value="Brr2_helicase_PWI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048863">
        <property type="entry name" value="BRR2_plug"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF4">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21188">
        <property type="entry name" value="BRR2_plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18149">
        <property type="entry name" value="Helicase_PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing helicase BRR2-like plug" evidence="8">
        <location>
            <begin position="129"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Brr2 N-terminal helicase PWI" evidence="7">
        <location>
            <begin position="312"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="DEAD/DEAH-box helicase" evidence="6">
        <location>
            <begin position="534"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="228"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="426"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="228"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="261"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="278"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="294"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="438"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF21188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2069048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="627" mass="70805" checksum="31D313AD24B45678" modified="2022-05-25" version="1" fragment="single">MSESYARSKQFEYKANSNLVIHSERNINDLKEPKGEPETLWGKLRGQMGDRVNYSKPAELLEKMQTLKRKNNEKLGGVDATQDTVVPSKKSKGTGGGGGAGYHHGEQHTDVLSATESFQGLYRPKTRETRVVYDKLLTFINGYIGDHTTEIIKGAADEIIAILKNDLIRAPEKKTEISKLLKGMSEEKFADLTNLGKAITDYKDQSSGVSSGTNLDSLDDTQGVAVVIDEDEDEENLSDFEIRDESDDDDNEDVGKLDSNNNDDDNEDKQEEQENMVDSENHLPTKTTSSDSSIKLKEEKEKEKQQQSSFDKSLINPLDIDAFWIQRKISTFEQDPHIAQKLAEQVLDILRQQDSRKCENDLVGLFDFSRFDFIKLLLNNKSTVLYCTLLAKSENEQERKKIEDEMSNDPILYTVLNQIKGIDIKGGNSNSKNDGKKQQASTSSTATTKQEEVIKKKKILNLDELSFQQGSHLMTNKNFKFPKGSKREQHKGYEEIHVPAKANPPFLNEERLIKIEEMPEWSHKAFAGLTSLNRVQSRLYEWAFKSNDNLLLSAPTSAGKTNVAMLTILHEIGKHIDQYGNIDRDAFKIVYIAPMKSLVQEMVGNFSKRLEAYNIVVKELTGDQSLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLR0</accession>
    <name>A0A8J4PLR0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_009391</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000696">
        <property type="protein sequence ID" value="KAF2069293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLR0"/>
    <dbReference type="OrthoDB" id="19593at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021629">
        <property type="entry name" value="Mediator_Med23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12691">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12691:SF10">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2069293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="586" mass="67088" checksum="E416DFFCD2F5353D" modified="2022-05-25" version="1" fragment="single">MTLPQPSGTQQQQQLNTPTSNVQNLNTPLSNSGGINNSSGGGGITPQSSSSSSSSQQQHSQSSSTSQQSSDIIDSFLKQQSEQLTTILNNNILASINNNLNLINSRNNSESEIFLVLDHCKQKINQIILSNISVSVALKTKLFFFIVNFQNSILKTFTNTSLKEAELLQKFYVWKNVSTYLKDHLLYFSKWKEFLYLGIQAMNSYPLIHPAIQKLCFEDTLDFFKLLVSFSHLEYPVYTILKEIKLFNSHACLEFQELVIPFYNRANRLSNLLTLRGLPDTFRLVGTTISLNQDIMIPYTSQLTESPPFFTTNKPLNEKYSVIVSHMLSQGAGVDSIKEFLFPQVQSNTLRSKIINIITEAAILRTVQLLHKYENNTNESNKYQIITQSWKEWVAFSDQFFSLVEENLLDFEQFIMLLQNKRMLSGKPIKDNIVIYLLSKTISNETKVMFNLDLQKVNGNHLGFLIPSILSFLNESQFNDPYLITQNISMHFFLMRILTIIEGSQDSIIPQYIKRLLEQQPQAFYPHKNKWEIEKSKPRMEFFSSNEDSTLISVVSNFFPFEVESELMKFQTSGENGVFSNGEHII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM49</accession>
    <name>A0A8J4PM49_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000634">
        <property type="protein sequence ID" value="KAF2069594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM49"/>
    <dbReference type="OrthoDB" id="46564at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF151">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="240" mass="27429" checksum="F02D264249A21294" modified="2022-05-25" version="1">MEQENDNLDIDVGDLFLNKSYTKKPIEYFGVDILVNVLNSASTDFDLTGQVIWPAAQVLTQYIISNQHEYRDKNILEVGSGVGVCGLFLARLGQPCTLSDNNETVMELLRMNVQESIDRGYPCKEVTLDWGVEKDINNCLSSDPNGYDYIIGSDVVYWQTSIVPLFETVDKLLHKSSTSRFIICYQSRSTQTDNYLLKKAQEYGFSFEFIPIESFLNRDYLTFTDSTISIINLIIFKRNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMD2</accession>
    <name>A0A8J4PMD2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Acyl-coenzyme A thioesterase 13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Hotdog-fold thioesterase superfamily member 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Thioesterase superfamily member 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">CYY_008991</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels. Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Can also hydrolyze 3-hydroxyphenylacetyl-CoA and 3,4-dihydroxyphenylacetyl-CoA (in vitro). May play a role in controlling adaptive thermogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a fatty acyl-CoA + H2O = a fatty acid + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16781"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:77636"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:16782"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homotetramer. Interacts with PCTP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the thioesterase PaaI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000614">
        <property type="protein sequence ID" value="KAF2069683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMD2"/>
    <dbReference type="OrthoDB" id="46529at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000021">
        <property type="entry name" value="Acyl-coenzyme A thioesterase 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039298">
        <property type="entry name" value="ACOT13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003736">
        <property type="entry name" value="PAAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00369">
        <property type="entry name" value="unchar_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660:SF1">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660">
        <property type="entry name" value="THIOESTERASE SUPERFAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioesterase" evidence="19">
        <location>
            <begin position="61"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00064709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00067273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00083956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAF2069683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="155" mass="17280" checksum="F4E525F0AC452614" modified="2022-05-25" version="1">MEKQTQEELNKIKEKLDGLLEKWIGFNQYDANLLSKIKLVKWEKGSVVYSMNVDPELCNMFGTLHGGAQATLVDVLGSMAIFSLDPTPNTIVPSVSVEISVNYASSAPRDSTVYIHSNCYKQGKNLVFTETTIKNDKDEVVCKGSHTKFVLKSKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMR2</accession>
    <name>A0A8J4PMR2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Vacuolar protein sorting-associated protein 16 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_008868</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the VPS16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000590">
        <property type="protein sequence ID" value="KAF2069817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMR2"/>
    <dbReference type="OrthoDB" id="1792at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.780:FF:000001">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 16 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.780">
        <property type="entry name" value="Vps16, C-terminal region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016534">
        <property type="entry name" value="VPS16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006925">
        <property type="entry name" value="Vps16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038132">
        <property type="entry name" value="Vps16_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006926">
        <property type="entry name" value="Vps16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12811">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12811:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04840">
        <property type="entry name" value="Vps16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04841">
        <property type="entry name" value="Vps16_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007949">
        <property type="entry name" value="VPS16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vps16 N-terminal" evidence="4">
        <location>
            <begin position="3"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Vps16 C-terminal" evidence="3">
        <location>
            <begin position="507"/>
            <end position="821"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR007949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2069817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="829" mass="94394" checksum="7D37FA2992AEF8E9" modified="2022-05-25" version="1">MITSHWNTIGNSTYSKREIYNLTWDVDLTQQIAVAAPFAGPIAVMRDPTKFIEVNSQNMRPYLRIYTASGQEISSIPWDSTKGSVVAIDWIEKERLVVVLQAGLVLVFNVFCEQILQFTLGDAVKLEDIVECKIWSDGIVVYTSASNFYSVPSINDHFMGCGRVQKMSSIPEEPTHRPGWAILEPHFTLSQTVELFVSVNSSLYIIDEDKTDQQLDSLGIILKMCISPCGKKLACFEQKGTLWIIGTDGNTTNPFKFETGATKPPVIKWCGSEGVMMYWETTKDPVLLFSAGDSWVKFSPEQPVSLVTEIDGLRIISNQSSEFFHKVSDVSYDIFKIGSTSPAAMLYDATDHFTSKSPRADESIRSIKSDLEQAVNSCIQAAGFEFNGSDQSKLLKAASFGKCFLDNYNPNYFVNMCRSLRVLNAVRHYEIGIPLTIQQYRYIGPDELIERLINRRKHLLAWRICDYLKIKSDVVLNHWACTKVRTDIEDAELSKIIIKKLESVPGISFANIASAAYLAGRRKLAIKLLEYEPKAADQVPPLIKMGEREMALNKAIESGDTDLVYLVLLDMKRNLPLHDFLEVAFSKMVALDLLISYCKQKRDLALLKEIYDIKEQSKEMANIFLYEGFRNPDFDGRLKGYQKAIDCYHTSKDKDDQLYGKLTEEQIKLEVLQKELEGSLQEPFSGMSISDTIFKLISLNQGKRANSLKSEFKVPDKRFWWIKIKALSILNDWEELMKFSKEKKSPIGYEPFVEVCLEQKNQVEALKYIPKIQDTLARVQFFIQINYFREAAETAFKDKNADLLNLVAKKCTNEEVSKSIEQMRSQLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMU5</accession>
    <name>A0A8J4PMU5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TTI1 C-terminal TPR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008799</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000580">
        <property type="protein sequence ID" value="KAF2069877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMU5"/>
    <dbReference type="OrthoDB" id="49511at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052587">
        <property type="entry name" value="TELO2-interacting_protein_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057567">
        <property type="entry name" value="TPR_TTI1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049362">
        <property type="entry name" value="TTI1_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18460">
        <property type="entry name" value="TEL2 INTERACTING PROTEIN 1 TTI1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18460:SF3">
        <property type="entry name" value="TELO2-INTERACTING PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24181">
        <property type="entry name" value="TPR_TTI1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21547">
        <property type="entry name" value="TTI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TTI1 C-terminal TPR" evidence="3">
        <location>
            <begin position="224"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="260"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="359"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="204"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2069877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="597" mass="68711" checksum="FD533066E705CCAD" modified="2022-05-25" version="1" fragment="single">NNNNNNNNNNNNNNNNNNLNSISSTKHLKEQSIYLDNTILISLIIDCIGVMSKVIPINPIKYKQIFISKVIYQVLEKYGMAGSDKTNMISNSCLTTLTSIMTSFKYQSLQDIVYKNSDYLLDTIENHMRYLESFPNTPNVFEGILNITGFGFLPFLSDTVKMMLQSLDIASTTTGVSQQRKNIQIYIKILLKIIIVLYNNASHQIQYQNELNNLREQLIKNNKNDNNNNSNQEEQEEIINQENRSIQDIKQFFLDHHQNQKEKQDKRHITTNPSNLDDGDDNSGSNDILNHNNIIPLPSDFGIPDFQETTELSRQLVQDILEKCIHFVSSKNRLIKLDVIDIIDKSLTIISTGNRKYGSQEIEEDQNDVNNNDDSNNNNESNNNNKSNIGLKNTKPKIALYPMIHLVWPSIVKRVSEPDRVVCKRALKVIITISNLSTDFISQRFWEQLWPILSNVLIEENVLSNQKDLNEKRKQQNSLFTPSYKIQHSILSTLSIVLGTCTISQDQILQVARDTLVYLDSHQPESFQQFTREIWKFLLLHDPDSLFLLLFHLSLGRDQNPMYHLSPNSNLIDIPTFKITSNDFKSNAILLFQQINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMZ4</accession>
    <name>A0A8J4PMZ4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009344</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000684">
        <property type="protein sequence ID" value="KAF2069336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMZ4"/>
    <dbReference type="OrthoDB" id="1062969at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="441" mass="47769" checksum="B492063593346494" modified="2022-05-25" version="1">MTQTQVRINHPDDLANHFHLFGKLTIVGNAVDEIQKYRATHAAAGLSTPRAVNDDSNTTNVTPAKQPALLTTSEVKAAYQTNNFAKMKDLETKANNDLLPVYTNITDKGDSAWVKVRASNGQEYYMSWSATASAWSYTESIKAVNDARQAADPATKYEAVVQFGTYSASTTIAGIHSYNMGLTTMVVESVIAMILAKCISQFIADGLGFLVAQFAVRLASAAAELGLESFSFAVPEFLIGAVAGCLCFAIIFIGIAYLWNWLNKQYTLRVQVFNWDTANDWTLNTQAKDNAVNPGADSDTNQLNIKIDKMTTPGNTVTPPGFGGGVTSLDTVCYYSIIIYQNSSTFAQGLSFAFNASRDSSTGFSIAFTCPRFSDNAMYMQPSVVDPSAFLKQASTHWQSNPQYINVYPGGVIPIGAKFDALSGASDDLYNLNININQAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN37</accession>
    <name>A0A8J4PN37_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chaperonin Cpn10 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_007733</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the GroES chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000428">
        <property type="protein sequence ID" value="KAF2070960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN37"/>
    <dbReference type="OrthoDB" id="184876at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00320">
        <property type="entry name" value="cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.33.40">
        <property type="entry name" value="GroES chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020818">
        <property type="entry name" value="Chaperonin_GroES"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037124">
        <property type="entry name" value="Chaperonin_GroES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772">
        <property type="entry name" value="10 KDA HEAT SHOCK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772:SF0">
        <property type="entry name" value="10 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00166">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00297">
        <property type="entry name" value="CHAPERONIN10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00883">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="103" mass="11041" checksum="5625A010A288FBFE" modified="2022-05-25" version="1">MSGLKRFIPLLDRVLVERLAADANAKTSGGIFLPQTKTSNNSFAKVIAVGTGSHKSDGSFIPLLVKEGDTIIISPKAKPISIPSNEKTFELINEYDVLGVVEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNM8</accession>
    <name>A0A8J4PNM8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008103</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000472">
        <property type="protein sequence ID" value="KAF2070584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNM8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="400" mass="45227" checksum="6F7079AED840C442" modified="2022-05-25" version="1">MCSFILIWRNKYLKDIIIEYLYALLESSVKCSSSSPINRYRHYCLSVDSQYDIQFINRYDRPRALIFNHYFSVQPHKLIDIVPRSVTRLKVELERNNPIPPWVTTLFLKEWTAVKNGDIPKSVTDLTLCSMFRSDNLMLLKGKIPGSVKKLAIPSLDKEISHIIPKSVTHLTLLKQPINSINDRYLPSWVKHITLGTLCVSDADSHSITPSLSARLGGTYFICTSATRANIPRNTTHLIWMDYTDDQGISAANVPSGVHTLILGEKYNAPIQSLPPSITQMIFTATFELPLSSISFPPTLKHLRISLPKETVIKASSFPATPLLSHLSLDQQCFIESLPPSVHHLQLHSGYTGNKSFPSTVKHLKVISINQFMDIPSTIQSVCAYSPSYFTYPHPRDNEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNT0</accession>
    <name>A0A8J4PNT0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Acetyl-CoA carboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">CYY_008806</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP = N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13501"/>
            <dbReference type="Rhea" id="RHEA-COMP:10505"/>
            <dbReference type="Rhea" id="RHEA-COMP:10506"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83144"/>
            <dbReference type="ChEBI" id="CHEBI:83145"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.14"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>hydrogencarbonate + acetyl-CoA + ATP = malonyl-CoA + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11308"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000581">
        <property type="protein sequence ID" value="KAF2069876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="14612at2759"/>
    <dbReference type="UniPathway" id="UPA00655">
        <property type="reaction ID" value="UER00711"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004075">
        <property type="term" value="F:biotin carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001295">
        <property type="term" value="P:malonyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000005">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000003">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000005">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000010">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.460.10">
        <property type="entry name" value="Biotin dependent carboxylase carboxyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1770.10">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049076">
        <property type="entry name" value="ACCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049074">
        <property type="entry name" value="ACCA_BT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013537">
        <property type="entry name" value="AcCoA_COase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728:SF3">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08326">
        <property type="entry name" value="ACC_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21385">
        <property type="entry name" value="ACCA_BT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0092">Biotin</keyword>
    <keyword evidence="10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="18">
        <location>
            <begin position="17"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="17">
        <location>
            <begin position="170"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="16">
        <location>
            <begin position="642"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="19">
        <location>
            <begin position="1451"/>
            <end position="1809"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="20">
        <location>
            <begin position="1813"/>
            <end position="2135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAF2069876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="23">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2238" mass="251601" checksum="4C2E1009D8AE707D" modified="2022-05-25" version="1">MIQELETYIKSHGGNKYIDKILIANNGIAAVKAIRSVRKWAYSNFGNERIIKFVVMATPEDMKANAEYIRMADQILEVPGGTNNNNYANVDVIVDLAERAGVQAVWAGWGHASENPRLPDMLSKTQNKISFIGPPAGPMQDLGDKIASTIVAQSAQVTCVPWSGSGLTVEYAKTGVPEQVLRAATIATVEEAIQVAGKVGFPAMIKASEGGGGKGIRKVLSMEDLPAGFRQVQSEVPGSPIFFMKLVSNARHLEVQIIADQHGEAISLNGRDCSVQRRHQKIIEEGPPLAPTPETWESMERAAVRLVKEVGYVGAGTVEYLFADNDYYFLELNPRLQVEHPVTEEITGVNLPATQLQISMGIPLFRIPDIRKLYNEKPNESSMIDLQDYKKRAAPRNHCIAVRITGENPDEGFKPTSGQIHELIFRSTPNIWGYFSVGPKGGLHEYADSQFGHIFASGPTREEARRTIILGLKEISIRGEIRTPVEYIIHLLETDSFKQGEIHTGWLDQLIIQKVKTIKPNTMVVVTCGALYKASSTFTLRQQEFSHQVHYGQLPSLELLQNTVPVELIYENIKYQFEVSRTGANSYSISLLKDKSIFIDANIQLLSDGGLLILLNGATHVCYGREDVTGLCLIIDSKTCVFSEEYDPSVLRTSSPGKLVRFLVDDGSLVSKGAPYAEIEVMKMYMPVTAPEKGRIRFVLSEGSVMSAGAIIANLELEDSTSIQKSTPFSGTLVKMSPPQLIGEKPHQLLKHTVNNVKNVLCGYESFNLQSIVDSLFIQINDPLLPVFEFKEALSVIQSRIPSNLFQLISQFINQIPFTDSKLLAKDISLYLNKLLLENESLSNTISATIKPILDLTEKYHDGVEAAGIDLIKHFLEEFILIESDIQHRNIQTVLKQVRQTFKDNTQKVFDIALSLHSQSKKYKFIMILLQKISDSNLVIQFMEPLKKLSTMGGSSMDISLKAKQIIVRSQLPSSNEKLKNLESNIQSIIDTPSKDEDERLKKILNISRQSNEISDVLIPFFSTNYSDEVRRLAMEIYVRHSYRSYHVEDSKVTINNNDPTDFSFIEWFFYITLPNSGSVSPTLSPLIRSSSGTLPSPRPMSIFNGLSMIRTDSTDNITAMEDQTKLRYGMMVNFDSIESFGDNLSKVLKVYNNGEFSKARNNNSPIGGESTDILSVILNYYPESISQENHTIQSIVTILRSYVKELNNAHIRRITFICSSTSSPLKYFTFRERHLYLEDPIFRHIEPAMAYHLEVRKLNNYDITHIPTESQRIHLYYAQEKGKREMDPDADRCFFVRSVIRYSDLYGQSNDIKVDILLSQVETLLSESIEALEIAMSNKKYSRAQNHSIFLNIMPDVNFDEQMIGYVVQEIGDRLGKKLWKLRVGRVEVRGKIRKENSLIPVRLFIQNPTGYAFQVQCYYEQLNSAGQQVFTAVPGSQRGIWEGLPIDTPYPIMDAVQRNRFKAQRLDTTYVYDFPDLFREALQNIWMEYMEANKNNTVKVYPSPRGVLEFVELILAPGTSTDFPPTIPYDQLPMDQRPKLEETYRPVGYNDIGMVAWKMTFYTPEYPQGREAIVISNDITHQIGSFGPQEDLLFKLASELARKEGIPRIYLSSNSGARIGLADEVKNKFKVIWNNESDPSKGIKALYLDHQDYVKLSETNSIDAKQDPTNPDRWIISDIIGQKNGIGVENLSWSGLIAGETSQAYDEIFTITLVTGRSVGIGAYLVRLGQRTIQNDAPIILTGASALNKVLGKEVYESNQQLGGSQIMYPNGVSHISVNDELHGITSILNWIAFVPKSRGEMVPIIPPIDPIDRDIDFDPVVSGTKYDTRHLIQGVTSEHDPSVWISGMFDKDSFQETLGGWANTVITGRARLGGIPIGVIAVETKSIEKVIPADPANPLSQEQVSTQAAQVWYPDSSFKTAQAIADFNKGEQLPLLILANWRGFSGGMRDMFDEILKFGSMIVDNLRNYKQPVFVYIPPGGELRGGAWVVLDSTINLEMMEMYAAQDSRGGVLEPNGIVEIKYRDPDLIKTMHRLDAKLIEWDKEIPLGVSVNSLDEKQRAIKAQILCREKELLGIYQQIAIKFADLHDTPGRMKAKGVISQVVPWKNARKYFYQRLRRRIYQEHQINLMAKVYPTMNRLNRLELLDSWLKEITGDKYVTGNDNDEFIANSIYNNLGLLSDRITTLSSQYIQSQIIQYSETDSEATLDAIISILPTLSIEKKNLLLSLLSSSSDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPK3</accession>
    <name>A0A8J4PPK3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007188</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000369">
        <property type="protein sequence ID" value="KAF2071493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPK3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="2">
        <location>
            <begin position="67"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="516" mass="59982" checksum="8F033C4C42D5B34E" modified="2022-05-25" version="1">MDPKKINCEFCSSVLDNPCRLLNCNHVVCNHHIELSLNNDFYKCPINDCLIQNYNFEKEPIEEIKLYKELNCLYHRNAWSLYCKNCNLVLCQSCILDHPPSHHIVGIGDQKFLEKAQKDKSKIDILLENSKDSLDSRTKLFEKKIEQYNELESTIDLYMEGLQTSFLNTIGLLRNHIKQDIKTKCLEATTKFNNLKKIQNSILGTSGTLSPKPTGIPDDLSKVRQYQDSLNSYQQLGQTTTIAEYHIHGYLSEFENVFKQINQNIIIPIENRRQDTVVLPISISNLQTTHQKDIYVERFNNNINPTILSKLDYKIDNPIQSFSTLHLQGSIGIGKTSALLNFGLYCLEKSLFKYIFYIRCGDFYLKDLKTILLNYFFVDEEELRDEACVKQQFKNINHESTLLLFDNTLYNVLLLNDLMFFEQNGIKMIIASRLDQNSKDLDTSKNKLVLEPFTIPESIQLMKSCLNRDFPNHQLEEISKLLHFNPQKIKEECLILNSKSGLSMNEYIDNLKYVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPQ3</accession>
    <name>A0A8J4PPQ3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">mRNA-decapping enzyme-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_007462</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DCP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000399">
        <property type="protein sequence ID" value="KAF2071228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPQ3"/>
    <dbReference type="OrthoDB" id="440673at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09804">
        <property type="entry name" value="Dcp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010334">
        <property type="entry name" value="Dcp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16290:SF0">
        <property type="entry name" value="DECAPPING PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16290">
        <property type="entry name" value="TRANSCRIPTION FACTOR SMIF DECAPPING ENZYME DCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06058">
        <property type="entry name" value="DCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="126"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="138"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="212"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="234"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2071228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="327" mass="37458" checksum="CAD97756EE745E61" modified="2022-05-25" version="1">MNRDSTQSQSQQQKLSALQRLDNKIYSILGTSTHTTAYKFEETTKQWSRKDIEGALFVVNRTVEPYCKLIVLNRLSTKNLMEDITEKMIIKCSGPYLLYKNSEEINGIWFYENADQEKIFNLLKESQKNPPIPPPPQQQQQQQPQQQMLMPGIPPPGFMNTPNPNWPHMYMMQPQAMNQPPPHGVAGWPMPNNYMNQPQTPVSLESNHGNIPTTPLSPSSPLPVHHQSPSPSPSHHHHHHHHHQQPQQLTPNKINQEQEEHQQAITNNTVPLLSVLMNSVIPKNDDVENANGNGEILTKKQLQDSLRELVEDDKFITMVYNSYISDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPR8</accession>
    <name>A0A8J4PPR8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_007416</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000395">
        <property type="protein sequence ID" value="KAF2071258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPR8"/>
    <dbReference type="OrthoDB" id="1929172at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035165411" description="GOLD domain-containing protein" evidence="9">
        <location>
            <begin position="21"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="10">
        <location>
            <begin position="30"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2071258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="220" mass="25551" checksum="2AE9468FFC322661" modified="2022-05-25" version="1">MKGTLAVIALTLLLVQCSYSFMFEVIAAHEKCLTEEFRQDTLVNGKFEVSDRLSKNIAYPTDIVMNSMQMRFYVRDPNGDLPFDNHDARKGPFTFNAHVPGEYQFCFLDNYKPGALVMPLSRTVSLEYKTGVEANDFSDVVTKGQLKPSEAELRKIEDVVESIKEDIMYMKTREETMRNTNESTNSRVKWLSFFCFFLLIGSAFFQINYLKRYFKQRKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPS4</accession>
    <name>A0A8J4PPS4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_007033</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000353">
        <property type="protein sequence ID" value="KAF2071658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPS4"/>
    <dbReference type="OrthoDB" id="9999863at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140107">
        <property type="term" value="F:high-affinity potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051143">
        <property type="entry name" value="TrkH_K-transport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31064:SF30">
        <property type="entry name" value="HIGH-AFFINITY POTASSIUM TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31064">
        <property type="entry name" value="POTASSIUM TRANSPORT PROTEIN DDB_G0292412-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02386">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="86"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="302"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="374"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="432"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="494"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="537"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="567"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="138"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="147"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="162"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="183"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="199"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="229"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2071658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="641" mass="72486" checksum="42CF1F856F2E049F" modified="2022-05-25" version="1">MAILDIRFQIAKKKFKNFLIKYADRNHFYRAHFLYFVIVGFIGACIIQLIEGHHSGLHFSDSLFVSYSAMTNTGLTTVDISKWSDFSLFFVLILMILGSAVLLSLPIVLVRRYYLRKAYASCPPPSGNSRTSVHIEHYQTSSDNEDNAGNNSKSNNNNNGHVEQHHEFDDHSLSLFSHNDFSGTDDENSDSDDEDSNHHNNSNNNNNSISHSTMNVSQNSIGIPMDHPINSNNNNINNNDNNNNNNTAILNSNKNSNPPSLSEKDSIISIESSAIKKPKMKYQGVLPPTLEENLEYRSLGKLLYIIPLYIISIYTFAFFVMGIYIQSNHQAQQIMNENKVNGWWWALFHGVSGFTNSGLSLFSENLIPIHHNPFLLLMVSFLIIIGNTLFPVVLRQIIRVCEKFSKDPDPYTNLLENPRSIFTHLFPAKETFVLFVVWFIFNVFQISLMSILESNEKAFNGMSSGITFLNYYFQSISTRTCGFNSIDLTLLSESVLMLFLGLMFVSSYPFVISLKRSAVNGKYSGSDNQSREVMKDLLIRDLFIPYVCILLITILEEAKLDSQEILVFHIIFEVISAFGTVGLSVGLSHLLSTSSKLVFIIVMLLGKHRGLPDSIDTAVAIDMPISSYLKKYRRRKSALLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPU8</accession>
    <name>A0A8J4PPU8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008824</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000584">
        <property type="protein sequence ID" value="KAF2069859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPU8"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051948">
        <property type="entry name" value="Hsp70_co-chaperone_J-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360:SF3">
        <property type="entry name" value="J DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="8"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="375" mass="43399" checksum="F328CDBA1DC84EB4" modified="2022-05-25" version="1">MDKTQLPDLYEFMGISPEAKEDEIKKAYRKLAIKYHPDKNPGSDEKFKELNAVYEILSDPQKKRTYDLFRDSNMSMPEFAARTGQDYATLEREIEGFFCGSCIGILYSLIAMSVNIIFGIPLTTAFFPSMVFFISPVILSKGGSFSKGFAIGGYAGMISFPFVFTIQVLSISKKIIEKPIKYLIGLVDNSKVNNEQQLQTTLLLDEEDWEFINRKKTEYEKIDDIERNWTFLHNENEKKVDDTNNLKLFKSTTDNIFTTLKVIKEHYQQKDNSFESDLILTKLTESLVYINDIKTIIRNVEDSFIEENVNSSDSSNQDQFSKVSTQITSVLKDSLMFVQSEKGSFHTNMRILTLLKNCELFLEEYVFNQFEYYQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ57</accession>
    <name>A0A8J4PQ57_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_007106</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000361">
        <property type="protein sequence ID" value="KAF2071573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ57"/>
    <dbReference type="OrthoDB" id="252020at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061574">
        <property type="term" value="C:ASAP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15481:SF0">
        <property type="entry name" value="LD23870P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15481">
        <property type="entry name" value="RIBONUCLEIC ACID BINDING PROTEIN S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="346"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="425"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="40"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="71"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="124"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="136"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="157"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="258"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="286"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="439"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2071573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="467" mass="52143" checksum="E2B4AA1674CBCDA7" modified="2022-05-25" version="1">MSRSPSPNRGISPPPRRSRSPMNRNSNKGGSRSRSRSNSRSRSPMKVNRGGSRSRSRSPLNSSNRNNSRSPLRRNNNKSRSRSRSPPRSISPRRNSNRNGSKSRSPIRGGGSRSRSRSRGGVRGNNNNSNNNNNRSRSRSPPHRRMSSRSRSPPLRSRSRSPMRGGSRSRSRSPPYRGGGGRGGGRGRSPPKRRRSRSPPHSPRSRGGRGGGGGYRRDMSISPPPYRGGGGRGGRGRSPPPHYRNGGRRGRSPSPPPHYRRKASRSRSRSPPSSYRPRSRSPLHKNSNNNPTGASSTSPPSTTTSNTAKTHSRSRSRSKSPMPQKQKDRSRSRSRSRSRSRTPSLSPIRVEKLTPNTTKEHVREIFENFGTVKDVNYLLDKEKNNQPLGIAIIYFIKKDERDKAKKYMDGAKIDSSVISVRFNVPKPQYHKRGGGSGGDYRNNNRDNNNGGGRHYRKNSPPPRFRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQE6</accession>
    <name>A0A8J4PQE6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_007704</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transcription factor STAT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000426">
        <property type="protein sequence ID" value="KAF2070971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQE6"/>
    <dbReference type="OrthoDB" id="19300at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09919">
        <property type="entry name" value="SH2_STAT_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000446">
        <property type="entry name" value="Signal transducer and activator of transcription"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.340:FF:000007">
        <property type="entry name" value="Signal transducer and activator of transcription"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000099">
        <property type="entry name" value="Signal transducer and activator of transcription"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.340">
        <property type="entry name" value="Rel homology domain (RHD), DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.240">
        <property type="entry name" value="STAT, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041604">
        <property type="entry name" value="EF-hand_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037059">
        <property type="entry name" value="RHD_DNA_bind_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001217">
        <property type="entry name" value="STAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015988">
        <property type="entry name" value="STAT_TF_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015347">
        <property type="entry name" value="STAT_TF_homologue_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041410">
        <property type="entry name" value="STATa_Ig"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11801">
        <property type="entry name" value="SIGNAL TRANSDUCER AND ACTIVATOR OF TRANSCRIPTION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09267">
        <property type="entry name" value="Dict-STAT-coil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17901">
        <property type="entry name" value="EF-hand_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18214">
        <property type="entry name" value="STATa_Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47655">
        <property type="entry name" value="STAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SH2" evidence="10">
        <location>
            <begin position="443"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="548"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="29"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="50"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="65"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2070971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="567" mass="64191" checksum="1BF60300BE586FF7" modified="2022-05-25" version="1" fragment="single">TKNHLQQSMNQQPQQHQQQPQTPQQQQQYLNHSSNGNQSPLHISASSQPPSSPLSSPLPSPTPFSRPNIINSSQQQIPIGMQQQQQQQPIMQQQQQQQNQVSTPQPILDTIYKLLSEQEQTLVQMIHEQSILLNRLPPTLDENSVQLLKNLSQKQITLSNQMNTEMSALDATKKGMILEPTDLAKLFALKQDLQIQFKQLSLLHNEIQAILNPQHASTKPNVALVLKSQPFPVVISKGKQLGENQLVVLILTGARSNFHINGPVKATMICDSQPTTKNNPTTPLEMDSQPIYPATLTAHFPLKFLAGTRKCSVNLKFGVNIRDMDNTTTTVESDASNPFVVITNECQWEGSAGVLLKKDAFDGQLEITWPQFINTLQRHFLIATKQDPVRPKRPLSAFDFKYIQTHFFGNRTIIHQQDFDKFWSWFGKSMQTLRYQRHISTLWQEGIIYGYMGRQEVNDALQNQDPGTFIIRFSERNPGQFGIAYIGVEMPPRIKHYLVQPNDTAAAKKTFPDFLSEHSQFVNLLQWTKDQNGNPRFLKSHKDSALGSFAPKKLQPTPIGGYEPLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQH7</accession>
    <name>A0A8J4PQH7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Alkaline dihydroceramidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_006938</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alkaline ceramidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000342">
        <property type="protein sequence ID" value="KAF2071753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQH7"/>
    <dbReference type="OrthoDB" id="15687at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006672">
        <property type="term" value="P:ceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008901">
        <property type="entry name" value="ACER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46187">
        <property type="entry name" value="ALKALINE CERAMIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46187:SF3">
        <property type="entry name" value="ALKALINE CERAMIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05875">
        <property type="entry name" value="Ceramidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="114"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="214"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR608901-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR608901-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2071753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="295" mass="33675" checksum="AA2AFFCBE5485F83" modified="2022-05-25" version="1">MGHHHHHSIIDPSEYYWGEPTSNIDWCENNYVQSPYIAEFYNSVTSAIISAYALYGIYLLTRMHNSYHHNLMAKEGFIGRLNIAYFSLFLVGAGSVAFHATLLYQNQLFDELPMIYTSLILLYILMTVGETKNANGTYKGGFLGNSLLRHILPYLLVVYGLFITVTIIVIKDQPKILQISYGALVIYIVLHSAFLIEKRDSESISTQRSPDNYLYRYAFCAFLAGYALWVTERFFCQDGYVIVGLQLHAIWHILTGLGVFVWTQFLICKLLEAKGYKIQLQHFLGIPNVVAIGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQW0</accession>
    <name>A0A8J4PQW0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007141</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000364">
        <property type="protein sequence ID" value="KAF2071542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQW0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="267" mass="30424" checksum="A128CA9E7B0101B7" modified="2022-05-25" version="1">MEYNLGIGETIEVIFPQDSFHHIVGLHRVGTNESIETDTYFAPVITSYNCTRKNILIDDQTQKVSECIFKGSRLPTDKIISLVNGLNIYEIGGTSDCMKVNLIGAMVPRPFINIYILTTFKLFLEATGVKFIPSPFIVGGFLYEQVIHSDVILISFKGYYLDPKYFNVLYTNQATSYNLSNCIYVDQTSDKEYYYEELTCTLPSLGMSEFDGDLVDLVPILKEYFFENVTINPAHNFTKLNWQSTSSSLKPIYYIIFSIFAIIINLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRD0</accession>
    <name>A0A8J4PRD0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000365">
        <property type="protein sequence ID" value="KAF2071525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRD0"/>
    <dbReference type="OrthoDB" id="19907at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="313" mass="35765" checksum="A5223F6CE47D5AE6" modified="2022-05-25" version="1">MANDTFFVHNNNTNQQQQQQQQELSQENNESSYKCPHCEFFYTSNSLLPNLTGFGNNQCPHLLIDYTSVSTVNATYRSASNLTATMLGTTKIPLVIAKRFIRYIRDRNYNTPPGFYTIHQIKSRISNTFQGITSFSIKKTIQESNLQDEKKEILIESSYYLTSNPIVDASQFESFIVKNYIDTDISSLFSINQFINNNDMMNNNSNNSSSDTNIIDSNSNNSSNNNSSLYYIAFNQDQLKTLFLEDDDQDLNFNSRWMVIFLPNQLPLFIFISNNLTTTESDKVFFGPLQISDSLVSNIKTLPIIHLPDVAVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRK9</accession>
    <name>A0A8J4PRK9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_005991</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000257">
        <property type="protein sequence ID" value="KAF2072685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRK9"/>
    <dbReference type="OrthoDB" id="21050at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="196"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="289"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2072685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="304" mass="34050" checksum="38FAB0C1FB3CCBCB" modified="2022-05-25" version="1">MSSANQDNNNNQSSLIHQQNSEKVFNQKCTTTVKSNGIANNSNNDKLLLSQQNSHDKVDYDLCGLFKIYFPLERNFENLDIKDHDQIKTTDSSINTISAGGNEKFIPVLDCPMSWSELTDLSESLYSTSSSSSSFIDFDELSQRSSSSISSSSSSSSISSSSCSSIKVSKKPTQQNSPSNNSSGNNTIGNSKKGSKKLFIGGITFDDIKDKSDLIELRIKKIKHLFESFGKVTRFDEHWDKEYCFVTFVNPKITLHVFQSISNQNKKMKLINTIKESLIHENNDPMAAPSNTLYTKYPNNRKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS01</accession>
    <name>A0A8J4PS01_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Right handed beta helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005296</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000207">
        <property type="protein sequence ID" value="KAF2073383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS01"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035252357" description="Right handed beta helix domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="480"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="487"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="533" mass="59455" checksum="B69D4580264D2CC9" modified="2022-05-25" version="1">MAWKICIGLLVLVWLGACMGQTVELTTMYVDLYSTNMDPSCGMTKSQACSSISAARDSFLLLPVAQQSVRQAPYVYSPLKIIMTPGTYGHANTKIDFFTFNVIIEAEIAGTVTITGLDMDDAFIVAPYAPQATNNYTYITINNIHFDQFDKQVVQIDRKAVSFVRFNGCIFSNSRDAITLSGSSESSSLVFNNCIVSKNRDGALVFASDVKVLMYNVTFSNNINYQIFKGYASGITLENTLVVENSYYYMENELWNGLINIFGGDLTILNTQWKENNSPFQIYARQYDDLTQSPHLSQPTRIAIASSQFINNNAETNPHSYSTLIYQGSFSGMLNITDSYLAYNRGNDVGAIYSDSETTVARTSFLNNFGAEAAQIYHRNQSYYQNCTFTYDDTIEYTESKSQLYMEHVPYIAFTDTVFIYTDTDIINIPLIQCKNSTIKFYDSRIKSSDSSSTYPIHCIWGCTKAYSRYKLGCERDLPTSAPKASPNLSPSNETAKSVTNETSSSSAHLTHPTSILLSICISLTISICSSHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT10</accession>
    <name>A0A8J4PT10_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_005735</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000237">
        <property type="protein sequence ID" value="KAF2072942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT10"/>
    <dbReference type="OrthoDB" id="18453at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000444">
        <property type="term" value="C:MIS12/MIND type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007128">
        <property type="entry name" value="PMF1/Nnf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15459">
        <property type="entry name" value="POLYAMINE-MODULATED FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15459:SF3">
        <property type="entry name" value="POLYAMINE-MODULATED FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03980">
        <property type="entry name" value="Nnf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-0137">Centromere</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="19"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2072942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="223" mass="25780" checksum="D65DCCB04A546E1A" modified="2022-05-25" version="1">MSNNNSNSNNNNSNSNSSGLNDSVTSSNTEQSRNASPDILEYFNFEECDAIRVKKLMLFHEYIVEKTLSEFPFEIFIKLYAKLSPLNIDPYLTAVYNNIVLDSLQTAIRNDFQMICNERNVIPKLIELEKLIRDQPTLLTTGKRCPPMLNYSPDEIIKSNIVELKIQERVKLVAQYKTILEEKNQMNKTLVDLETRKLELMNLIEQKCKNISHIVEQSKTLDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT29</accession>
    <name>A0A8J4PT29_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Right handed beta helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_009760</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell outer membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000798">
        <property type="protein sequence ID" value="KAF2068921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT29"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003368">
        <property type="entry name" value="POMP_repeat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01376">
        <property type="entry name" value="POMP_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02415">
        <property type="entry name" value="Chlam_PMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2068921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="32923" checksum="0CB88E6A7E0C950E" modified="2022-05-25" version="1" fragment="single">DHRSFSLQEFYESWLYIFGDQFESSSKFKNPTIVMKPGVYKGLQNTNLMISSSVNIKSTSGSTKTLIDCEGIGPGIMVNNEYFSLSGFSVSNCRGNLGGALKIDSEMTTFSDLQIFNNLASYGSGLYVTSKSVTIKDSSFVNNTALEGGGAIYSNSSTISFENTIIGCNSNSSKYDIIATKKSYLEFDVVAIDIIGTSCEDESTIVNSNGDSLCFKTGLCKSSTTTPPQVDESYGKFTCNFDGQCDIFTENCLSCPNDCQQCRLKAWKLEYFSDHIPDVHPLQNGSITSVPTSNLTNFMKGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT46</accession>
    <name>A0A8J4PT46_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_005680</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESF2/ABP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000233">
        <property type="protein sequence ID" value="KAF2073002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT46"/>
    <dbReference type="OrthoDB" id="287393at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034462">
        <property type="term" value="P:small-subunit processome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12263">
        <property type="entry name" value="RRM_ABT1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039119">
        <property type="entry name" value="ABT1/Esf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034353">
        <property type="entry name" value="ABT1/ESF2_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12311">
        <property type="entry name" value="ACTIVATOR OF BASAL TRANSCRIPTION 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12311:SF7">
        <property type="entry name" value="ACTIVATOR OF BASAL TRANSCRIPTION 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="106"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="26"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2073002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="281" mass="33275" checksum="61B9600332207EF0" modified="2022-05-25" version="1">MANKRKISQIDTTKTNDKLKKKLKEEEEEEHIDDEIENEQEDDDMIEDDQDNIEEGDQDDDVEEGEEGEEEGGDSENNKKKKKVIKIKAMDPEKIQKLKEENENRGIVYMSTIPARMKPAKLKHLLMKYGNVNRMYLVRGNVERKQHRNDMFKEGWIEFNDKKEARRAATLLNNNPMGGKTRDIHRDCLWNLRYLPKFKWHHLQDKLTTQRMERDKKLKMEINRVRKENLALLEQVKISKHVTAKKSSKDKVVRTFKQRSFHEGNNETLSNSIKKSVISNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTS4</accession>
    <name>A0A8J4PTS4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005448</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000217">
        <property type="protein sequence ID" value="KAF2073251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTS4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052050">
        <property type="entry name" value="SecEffector_AnkRepeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586:SF3">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140860">
        <property type="entry name" value="Pseudo ankyrin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="692" mass="80020" checksum="469B9EA671735B46" modified="2022-05-25" version="1">MTMMDQYYKLIFRNKNLRNNIIQYIHVQNAVSKKYCDIQSVGWMIKNKHVGLLRDKLAQKEYLYLVPEDIPLVFSLGMGLFKVVLERFNDYFPITEYSQYVYVSYAAANNDFELMKYFLLINGNIDSVFKSYDPIHGAAKSNNVEMVKYLFHNYSIRFPLGAILESLRHRNLELLTFFTSNKKHLAKTRHDEYYMQEIWKSSAEDLELFKLMEKTFKSKLRVDFPFIELAVKHYNLIEYILQNYNHQFLLTYSYPATRPPVISILESLTKSGSLKTIELLDRKGLLTHASSYAIIAHQALLSNQIGIYNYAKQKDPSISVNVALHQIEGVMKDVQLVVMYQEMGAQITSECVIQACTSGNLGVFVYIFESMNPTTSNSRIRIDKIDYTESIVKKCLASNNATVLEYLCKNGYLSTIDINLLHEYQIKIGKNSELELVKFILTHATKMDALSIYYILLEAALTSSVEVFKFVYPYHTESKLLNTEQIFNLVNAGAKKGCLEIVKLIMSSGNISLPPREVLFQSICSNFDMLSFFYDNHFPLVFHGVSISCKKALQTEQWECAKYLMNKFTVKYNEVLMGLLSCPNFELVQQFLAKCNPIPAEDHKRFYLSGVGNGYLDVAKHFYRPQVTVLIDTLLFVSVVQKTYVHMLEYIKSHVFTIPKTFSTLRLSKDLLYYDAYKPYIINYCGITPRSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTY3</accession>
    <name>A0A8J4PTY3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003979</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000133">
        <property type="protein sequence ID" value="KAF2074703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTY3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="194" mass="22203" checksum="F3542F4BDF476949" modified="2022-05-25" version="1">MDKTAELKKEIATLSNGFNELVNGINVKMFDLKFQIQKIDKLLVELDGQEPVVKKQKKEKKEEETTPEKPKKTRAAKKSATTASSPAKKRTLAKKDETQLNEGKRRKGKERKGGKGKKEKDEKEKKEKEAEKEKEEKEKKEKDEKEKKEKEAEKEKKAKATKKTAAKKEEAPKKVTKTKSSKKKSAKDSEEESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU54</accession>
    <name>A0A8J4PU54_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pre-mRNA-splicing factor SYF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_005042</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the crooked-neck family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000191">
        <property type="protein sequence ID" value="KAF2073657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU54"/>
    <dbReference type="OrthoDB" id="10067343at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000349">
        <property type="term" value="P:generation of catalytic spliceosome for first transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000023">
        <property type="entry name" value="Pre-mRNA-splicing factor SYF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000137">
        <property type="entry name" value="Pre-mRNA-splicing factor syf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000182">
        <property type="entry name" value="Pre-mRNA-splicing factor SYF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055433">
        <property type="entry name" value="HAT_Syf1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056350">
        <property type="entry name" value="HAT_Syf1_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055430">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045075">
        <property type="entry name" value="Syf1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246:SF5">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR SYF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23231">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23233">
        <property type="entry name" value="HAT_Syf1_CNRKL1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23220">
        <property type="entry name" value="HAT_Syf1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1-like N-terminal HAT-repeats" evidence="13">
        <location>
            <begin position="22"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA-splicing factor SYF1 central HAT repeats" evidence="11">
        <location>
            <begin position="183"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1/CRNKL1-like C-terminal HAT-repeats" evidence="12">
        <location>
            <begin position="390"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="754"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="435"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="754"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="798"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="810"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="822"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="836"/>
            <end position="846"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2073657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="846" mass="100441" checksum="8ADC2683E3E4D8A8" modified="2022-05-25" version="1">MPSTDQFIKIINEDIVVVPSIEDLPFEEDVGRNQYSVDSWLRYIEFKKESTQKERNFIYERAIRELPKSYKIWHQYLLERVENVRGSCVTDPLFESVNNVFERALVFLDKMPRIWIEYCEFLMLQEKITRTRKTFDRALISLPLTQHPRIWELYLKFILKKNVPSLTCIRVYKRYLKIEPEKVEEYIDYLIKIKEWQEASNQLLMVLEDDKFKSTKGKSKHDLWLLFSDILSSHPKEISQTIRVDPIIRGGIKRFTDQVGKLWCSLADYYIQLAQFEKARDIFEEALHAVVTARDFSFVWESYTQFEDSLIVAKQEILDENPSEESSLEFDIMIERYENLITRQPFLLSSVLLKQNPNNIQEWLKRVKLYQGNAKMIVQTYQEAIEAIDPQQPKGKLYTIWASFAHYYESNKKLTQARQVFENGLTINFKTVDDLASLYCEYAEMELRHKNFEQAIEILKRATVSPKKHIHIADSEPVQKRVFKSIKLWTFYADLEESFGTFHNTQSIYERMMQLKIVTPQIVLNYTRYLEENKYFEDAFKAYEQGIALFPFPHVQDLWITYITRFINRYGGSKLERVRDMFEQVLSKVPAKESIIFYLMYANYEEQYGLARHSMAVYDRATKNVDVQDRYKLYLLYINRASEYFGVTQTREIFTQAIENLPDQSARDICIKFADMERKYGEIDRARSIFVHGSQFSDPRTSPQFWSAWTDFEKLHGNEETFREMLRIKRSVLSKYLVLNPNLNLINQLNNIKNNNGNNGNQNQNNNNNSNNNQNKNNGIERRTVQPTINPTIQNEKEQEEEQEQESDTNIDKNIPKIKNEDEIDIDEEEDDNDGIDFKEMPKTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUL0</accession>
    <name>A0A8J4PUL0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_004453</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000157">
        <property type="protein sequence ID" value="KAF2074243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUL0"/>
    <dbReference type="OrthoDB" id="19324at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20617">
        <property type="entry name" value="CYP1_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000078">
        <property type="entry name" value="Probable cytochrome P450 515A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF201">
        <property type="entry name" value="CYTOCHROME P450 515B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12 13" id="KW-0349">Heme</keyword>
    <keyword evidence="9 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2074243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="479" mass="55049" checksum="471DEA86E1F42C8F" modified="2022-05-25" version="1">MEIITTLLLTIAVILFCSIYFKNKKRDDYPPGPFNLPFLGALYLIKQNQAHLGLHKLYKKYGKIFSMKFGDVDTIVISEPSIILEMFHNQGAKFYDRFITPSFEIVGGYRKNIGFTNGPHWKKIRGIVNASLTKSKTRQLEDLINFQFDKMHDSLELELKKTDIINMKTFFKKLTFNVIFNYCFGGFVPYEDESIPEDVKIFVLNSERLLSHLAAGKASDYIHCLKPFSSYQELHNIMDGIMSFIKPNVEYHIKTLDENKPRDFIDNLIIEIKNDPAKTIDVSCIQNICVDLLVGGTDTTATTLQWMILYLSNNPHYQEKLFQEIKSISPDGFPSLSDRGKFTFFNAVLKETFRRSPIVPLGLSHMVAEDAQVEQYFLPKGTQVLANFYSASLDESIWEEPLVFNPMRHFNSQSPVLVFSVGPRKCPGQNLSEDELFLIGTKIFKLFKFSRPSEELFDESPVMGVTLEAKFFNSKIELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUP0</accession>
    <name>A0A8J4PUP0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004397</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000154">
        <property type="protein sequence ID" value="KAF2074303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUP0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="449" mass="52543" checksum="13FA18CE8793E97C" modified="2022-05-25" version="1">MGFAFSKNPPLDKSTKFTNDAWLAINGQVFEKEEESIEIGDPSSIKIPFIIQVKEKLEMDKLDTESRQELDNVDDNEYVDDDPATDPVSIIFEALNTYPKLKSTDQFYLYTLFLPSTSSIQKIIDQSQGDAISKDKYRKFTKKEDLDEKSVVKKDIEKGDKGFPKCKTLHIFFTFQSKKDKAHYSDLLLFKDSEEETSASYSINEEQKVEIARYNNHTYFTLRDNPKAEKSTEEIEIPSYQDRVTSYFVDSDKQLFFSSTPNELKMRAIIILERILFPLFKILCEDDWRVECWDTTTSHLTQTSVINMAEKTFLMDCINEIADADEEIQNKFYATGQYRNLFEAQKFKFNSPIAPLTRDRDIIIEKPTTKDSFVESNPLRLGQYYDLFLKRNNKPWCHGKQNTLLCFGRIDPKKVKDSPNKEIKAIIDDIRYEILRYKKSRYISKSHRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUV4</accession>
    <name>A0A8J4PUV4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000193">
        <property type="protein sequence ID" value="KAF2073610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUV4"/>
    <dbReference type="OrthoDB" id="18211at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34958:SF1">
        <property type="entry name" value="ARMADILLO-LIKE HELICAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34958">
        <property type="entry name" value="CONDITIONAL LOSS-OF-GROWTH 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="182"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="219"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1037" mass="118263" checksum="7E6A11EEE2B71C96" modified="2022-05-25" version="1">MINANKKIINQIIRNVVQLNSIPDGTMPSPEASFSLNRYFDDPNTVDIVYVNMIDRVKNEKSHYEQVLSRILKLIKNNIALHPPSYQILLELDTFCLTLSSTNALLQKTITYLRALIANQVKNNPHYESIKKLHLSDNRSNKNNSIGLLRNRSHSASNSTGSNRDESIFQRSSLDLSNLLSTSVSSTSSNSSSSSSSSPLSLNDSPLRNTLLGGGAGGASSSQNGNNTNQNSNNNNNHHQHHHHDRNSSGGGGSGGHNQTFSPSLYSSNHQVLPLSASSSETGFKSTDNNNLNSSNGLKDSTSSISSNSPLVSATAYYNDGFIRDLISIKAVSQPSIPKIKSLLINSPYGGIQAEVKGKEAFRCPDDSSNWSSINVVRVTKKKIENKDLAILTFKKKKPTLFQYKYYKDQEALKITDAEINQVIRVIMNPATQQDRSIATKIFIKILLDIYCKNSFVDQATEKLITDYFVQMISNGNRECKIHTFNILFNLSIHINLYSEQRLEDGASSIISSSMINELQDSIFTILLALINHCIITREREEKVWSESLNCLMFFITEQGNIIKDRLYQLNPQIITVFLLNIQDLSDYTKRVLVRMLVNFLYKDQILNQQNNLFLNQDELNQIGGIDIILKLYTTIRSNEAKNNLFVIIFDYVLQTALKIQNIVDTQLTRESPLLLELFRRADAPQYFTQIFKCIPEKDFVSDFFLFFSNEHSKDEASFSYEDLIIKFSMRINEFAQRYQKVDQNFENYILDCQDNPEQCVELLNDWIKQNDDLYLFNAENWLFSLLKKVYVEKEGLNNPLYSFSSNIFVQLPTNSSPKVRKMYISLTERLILLLKYKLKYGESKPNDLFEMFNESFHRLLLVFGEKSENNILHVVDILFDLISGRSNSSSLRSKESKLDTNFSLFLSNQLVVSISSLKSININIIHYLFSNITESNNTREQRLFLLHLLIQRSHDSDDLLKIGGIVFFKNLLSDLNTQIAYHSSYFLLTQLEAESPEQYRTILTRLLTKARENNDENLISNPFFQVQGIIEMTHKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PV77</accession>
    <name>A0A8J4PV77_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ataxin-10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_004539</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000163">
        <property type="protein sequence ID" value="KAF2074136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PV77"/>
    <dbReference type="OrthoDB" id="379794at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051374">
        <property type="entry name" value="Ataxin-10/CTR86_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019156">
        <property type="entry name" value="Ataxin-10_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13255">
        <property type="entry name" value="ATAXIN-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13255:SF0">
        <property type="entry name" value="ATAXIN-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09759">
        <property type="entry name" value="Atx10homo_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ataxin-10" evidence="4">
        <location>
            <begin position="490"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="288"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="288"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2074136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="599" mass="68783" checksum="B9CC9569354B782F" modified="2022-05-25" version="1">MDNTKRMTESLRERLSLSLECRDTEWYQSCISDLTQLLSFSKDYENRVDIGSSRPFLDIVTNFVINNTAIDKYFNSDSSSSSGNDGDKLKLSLLSIRLLRNLCANVPQNQFEIISSEGLLDWVFDSLLFDQQHPANKHSSAIVNQCKSILQSTQQLLINMIVSNESNQNMLWYRCFPSNFLKLVKYHPLDVDQCTYQLTPGSLMLIYNCLLNNAKHRHQLSESKDLLEQLLFLLEQEDLEETGTEKQQQSDEYHNQIFHWIHLIFKSLFSNDCFIRCYQTLSSNYTTPTTTIESTPASEIPGQDDQDPDQPQDTNTRGRTNLYQIRLLKLLDAMVASNGKNVKNYIEKDSIIDVNCCFFLVYELSVLYNMDFNRRGQKEKLSNTTTLNDYDFQAIFFIIKIFANLTSYSEEMIHIAITKFKTDTTQSVQVLNIDDKSDKGDPNKHDLNTLLRKNGLVAICIGSLHGNYSSSTPTDKDTSNINCETEDKGFKKDLIRILGNLAYKNKANQDEIRELGGIELILNHCRFDVKNPYIKEWSVFAIRNLCEENQKNQMVIENLRNQGVANAEELESLGIKVEMQNGSIKFKSTRPPPNSTNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW96</accession>
    <name>A0A8J4PW96_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004418</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAPS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000155">
        <property type="protein sequence ID" value="KAF2074285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW96"/>
    <dbReference type="OrthoDB" id="295029at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007587">
        <property type="entry name" value="SAPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46345:SF8">
        <property type="entry name" value="FORMIN 3, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46345">
        <property type="entry name" value="INVERTED FORMIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04499">
        <property type="entry name" value="SAPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="16"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="777"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="835"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1036"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1078"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1179"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="777"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="790"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="835"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="854"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="902"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="966"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="992"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1036"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1078"/>
            <end position="1096"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1220" mass="135890" checksum="A2E0AC8E80444067" modified="2022-05-25" version="1">MEEDDEVKKINERRRLREEKKRKQEEEEKREQEEKLKREEERKLRKESRNKERAEKEEKQKKELEEYIKRSEDERRQRREERERKRLEEDRLAKEEEEKRRRLLEERERKRREEDEEKKKRLKELEESRKRILKELEDDSGSDADSQVDTILSLKKKRQAARARIESILASVDPQNNQYTTNGESVINMLLTDELKEEVIDLIAGTFRALSDYLSHPNSILTMVQYLSDVEMGNLVFNARQELRDGTASVEIKNSLARMQASYEILKGGIPIIAKIITDSTNYHILDQLFSLISNPPPISVTNMHIFIDLVSSFLSRKPDTLIYICDNKDTILPSLMQHLNNSETVDLLIKMIDVEMTQFNENSSSSSSSSSSFFGLFSSSSSSSSSSSSATNAKEKPNFTNELTPYLILRINEISNTDAIHEHLDLEIDNIHRLVIDIIKKYPKSKIAHLLCGQSFSSNILRNAFKLALSLPFTSPISSSILSLTCSLVEASIAFSLPSNIKGSVNSSSSLSSTGDLPSIINLLFKQIVVLDSNEPGGESRELSPLKAISEILTIKKNVSSNSTPEPFNIVKLKSLHLVNSMVKANISKLDREIWESGILKKCIDIFFEYETNNVIHCMVEQFLTLLIQRNLGSEDEEFMCHLLKDCSFVSRVVNILSGNDLNEPFDNSGNVVLYSGGGSIGSSSGSNLNSSSGSSSSKDVTGSGSLGYNNNISKLNGLFSGVFDKPSTSTAESESNIIGNNTIVPPAVIPMGSLRRGGDKPKIRRKVSFFSDDAENLAQEEEEDQSKEQQQTNNSNSSNSSNVEEIGTVLDDKNVSISSNSPSTDEALKKKLMTNSGTNSITAALTSTPPLKADKKKEEKEQKKKEKLDRKLKREEKRLLKRLKEKEREKEKEKKENGASKKSISSISSSSSSSSSSLSKSKNSNTTTTSSNHSSPSSSSPLSASPILLKKPTFSTDLPDDSSDTLSDSGSAISDSSSNGGSKVDQDLDNNNSNNNSNSTTSSTNTSTADMSKLSLNDFVSVDKDSILSSLSHLTTNDSTSTTTTTTTSTNTPVELDNIDVTELEQSVVVVCYGNENGNNAESTTSTTTTENCNIKNEEEQPQQEQEQEEKPKKKKVNHNIGHIISLCIEVTHIVNKSKNHLLRDIVDECCASQWDDVVVPKLRQEISYRNTKPIGTVSMRKGRKGGPTNPFANIESFPNTQNGVPIPPTEMTSKFFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWW8</accession>
    <name>A0A8J4PWW8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_003421</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000106">
        <property type="protein sequence ID" value="KAF2075292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWW8"/>
    <dbReference type="OrthoDB" id="449052at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02205">
        <property type="entry name" value="CBS_pair_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF36">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="90"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="335"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2075292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="406" mass="46244" checksum="F3AFACCF5531B479" modified="2022-05-25" version="1">MLNIRVNPGAFIENRLIKFKNSIVSTLSGEQSNNQYCFSNLHKEEEKYYQPINVSMTDEETTSPCNSSTTTNLENVQSLLELSIEDIPKLSSKNLITLKRSDSIDTAFKTLSTNDISCCPVMDPKFGFIGIIDTFDIVACVLSLFNKNESEKGKQMKNVGINYFSLSIDPISVVMRGSNINEQSKIMESNSVFSLLDAFDRYQLNRVMIYQDTRGTCDMYKPPVLSRIISQKDLFKWIWNNPSAKSQLIECCKSQTIESIYSKNPTFVTDSTQTIEVLKMLHISKISSIAVVNDKGRIKNEISTNSWKRMKGMNEKNIDLLFQDCSSFLQKTKKQHKEIYTCSPTTNIDIVWDLIARKGIRRVWVLNEKKLLIGVVTISDLFSHLKVLLKTYLDSIPSSFVTNSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX82</accession>
    <name>A0A8J4PX82_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tc1-like transposase DDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003197</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000097">
        <property type="protein sequence ID" value="KAF2075505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX82"/>
    <dbReference type="OrthoDB" id="10006939at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="462"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="540" mass="62334" checksum="7241FD83D0C78262" modified="2022-05-25" version="1">MIFRSAIDDNSDDNSQDTVAIDKGFNKNKDQTEEKKKRNYTKLTKDGRLRFLEEALSGESGIVSRLSEKYNISKQTGYNILEKYNGCSQSIETKKTGPKLGKNKKITREIGNFILNSIKEDNSLSGKQISNLIENKFIKSISISTINKFLKENDYTFKVSMQEEETRNTETLIQGRCEFAKLVLQDLKIDSMDSDKVIYWDVTYFLTNIISRKGRSKNVTPAVAPKEEENGGEEEGEEDVEDEENEREKEEIENDNKCQNIKYGNTTLPMISDQNIKGGYANTLGCCCAITGKEVLLVRTQFKHFTSDDFITFFIELLDLLEKRYPMTSFTIIGHNERINPNSFGFLVKPPYNRHIQNPKDSPFLNPIEYVFNQVKANVASQQKKDIGHLINSVKNAFNLVQPENLQNYFLTAQQFLHLCLQKQPIHAWRTKLVSKVSIPKNPSEQKWLFSHRTIFDSEINSLGRPLARDNEKNPPKPSKQEKEERKQKFAQREEDIQKKLEKEEKKRKKESECSQKVPKSTSKIPTKPNISRGRPEKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXR1</accession>
    <name>A0A8J4PXR1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C-CAP/cofactor C-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_003910</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TBCC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000130">
        <property type="protein sequence ID" value="KAF2074764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXR1"/>
    <dbReference type="OrthoDB" id="194775at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007023">
        <property type="term" value="P:post-chaperonin tubulin folding pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1250">
        <property type="entry name" value="Tubulin Binding Cofactor C, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017901">
        <property type="entry name" value="C-CAP_CF_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016098">
        <property type="entry name" value="CAP/MinC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027684">
        <property type="entry name" value="TBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031925">
        <property type="entry name" value="TBCC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038397">
        <property type="entry name" value="TBCC_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012945">
        <property type="entry name" value="Tubulin-bd_cofactor_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15139">
        <property type="entry name" value="TUBULIN FOLDING COFACTOR C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15139:SF0">
        <property type="entry name" value="TUBULIN-SPECIFIC CHAPERONE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07986">
        <property type="entry name" value="TBCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16752">
        <property type="entry name" value="TBCC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51329">
        <property type="entry name" value="C_CAP_COFACTOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-CAP/cofactor C-like" evidence="7">
        <location>
            <begin position="205"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="54"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00026055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2074764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="367" mass="41652" checksum="27EA184F6EBFAF29" modified="2022-05-25" version="1">MNNPNNNGNNKGSSTLDSISKEIEDRDEQRKLQQLRNKEIHQQTQIDLSNSNNITKCINEINQLEKEIENELQQIDLSTSSKDSTTTPLTIDSIKNTFNDIEHKIATMSLLLAESNHFLPLFEIKNLNLTISNVQKNTNTLKDKVIPKQKFSFKRAAKSSTPKVTNNTTIDTSIPSPAVAPLVDNESIFDNSNIKGFNGKELVYPPTATTSTSSSSDTINDLLISNLVDCKVILKMKVLTACKINNIKNCTIISHSPIDGSIFIDNCIDTTFSFSSRQIRIHYSSNCRFNIFVKSNPIIEGCNGIKFSSYPHRQLQLTDQDYSRFMQYNFDLININADTEKYKDVNDFDWLQTKPSPHWELVKEEQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXT0</accession>
    <name>A0A8J4PXT0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ARF/SAR superfamily protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_002657</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000075">
        <property type="protein sequence ID" value="KAF2076043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXT0"/>
    <dbReference type="OrthoDB" id="18085at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="129"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="129"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="317"/>
            <end position="324"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="445"/>
            <end position="448"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2076043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="503" mass="57219" checksum="FA603C775D283A7C" modified="2022-05-25" version="1">MSLSNQIMDFLHSVMEDIQHQQQPQQQQIYNIQQQQQAQFLYQQTPPPLHVELDSKDTSPTTAATILDGATPNTNLKMNQFIDLFQDNVNINNSDSPITNNDNSSSNTTSPIATTTTTSTLEQDLLLVQQQQQQQQTSSSQFNTPTTISSSSSSPSSSIPTSPSNNNLNDNNSNNTDNTGNGNDDIIPKKQQVARITTNNFLEALPQEIVNYIFLKVGANGVKDLMRVNKLCHKTGMNEKLWLYFVEHDFRLEKLEIGQYIKLNGGVRALYSNLYFKKKNSLPRAPSSNPLIQYFFRPISKIPALFSRKEYKALMYGLDGVGKTTMLYKFARGENVRTLHTNGYNVEIVEYKSCDFICWDIGYEKTNVPVWQYYIQDTQAIIFIIDSCDRQRLRLVKEELWNLVNDKNVQKIVNFKQQNNNTNNSNNNSNNKNTDTRVKVLIYSNKTDQISENPMNTLEVAQSLSLYNLPKNISWHVQGCSATSEEGDGLYEGLDWLSSQFDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PY65</accession>
    <name>A0A8J4PY65_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003146</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000095">
        <property type="protein sequence ID" value="KAF2075560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PY65"/>
    <dbReference type="OrthoDB" id="29523at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050656">
        <property type="entry name" value="PINX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149">
        <property type="entry name" value="G PATCH DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-patch" evidence="2">
        <location>
            <begin position="10"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="236"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="301" mass="35096" checksum="B1387160D33018BF" modified="2022-05-25" version="1">MDLSKFEGWMKKSGWKGEGLGKNEDGLVKAIHVERKNNTTGIGATRFQWDNLWWDNVYSSTSIKIGGDDQQDLFDTKESFVEETTTTVAVKKSSDKMSKVELKEYKRQTALEEQMERSKKKDNLYFGTFTKATLTLEAEIEQEEQDDTTENKDDDDSNNNDQQRPQEKLNDVSNDVFLACQGRVLRKYTPIGKLQRLKDQEEGKFSVESLNNNIKVYKSSENKIETTKSKRKLDDISSVVSNNDNNNKDDGEKPLKKSKKEKKEKKKRKRKKRKLVKKNQVKKKRNRNQNHRRHPKIKILV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYD4</accession>
    <name>A0A8J4PYD4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002462</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000068">
        <property type="protein sequence ID" value="KAF2076221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYD4"/>
    <dbReference type="OrthoDB" id="10263328at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="6">
        <location>
            <begin position="21"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2076221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="833" mass="92262" checksum="6B74A4AB739215B1" modified="2022-05-25" version="1">MDLLQALVNGQSSDENTRKHAEEFIQNASKINFPAFLHGLATELSKESNPEPTRQLAGIVLKNSLNSKNEEINAGLSSQWLAIDEAQRSVIKDILLQGLSSPAHLARHTCAMDVACIGLIEIPNNQWETLVPTLIENIKSTNDFLKEATLEALGFICQEIDPSIMTKYSNQVLTVITTGMKDPNAKVQLAGTRAMGHALEFVKSNFEIKEQRDYILKVIFDNANSQDSNVKQVAFECLVKIVTLYYEFILTYMIDLYNLTVSVIEKDPNEGVVLQAIEFWTSLAEEEQLLYDELDRKQLIIPQALTQLIPVMLKTLTKQEEIDDGWGVTQAGTTCISAMAEVSGDDILPHVYPFIQNNITSPEWRLREAACIALGSILQGPTNFNELPNLIPHLVNMLKDPNEMVKDTASWTIGRICNHQISAIPNYLEVLIKALIEATADPVSKIAAHACWAIHNICQAFEEGPIGPISSLAPAFNPLAQCLLNAAHRPKPDEDDRKLKTNAYEALNSLISFSGAPVEPIIEILKLTLNDFEQSLKLSTNNPNLNTETYDNVSNLQALLCSTLLAVSVTIKEAIEPFAEFMMTCLIQVFSNQSAIIYEEALLAIGSIIHALDVKFKPFVPKTLEILSKCLVIVEMGSVTSIAVAIVGDIARIMGKEFTPYCNEIVQLVLSVLKNPKSSMSSKPNALSCLGDIALSIGAGFGQFLQIVMPILNQASFIPVYDEEFLNELRCALFETYTSIIHGLKSDEQSAQIQPYMGDILRFVGHVYEDKDRYDTVTVAAIGLLGDIAQALGDPIRPALEHEVVKGFIKEGYEKNFEIAQWTQRYIFGNKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZH8</accession>
    <name>A0A8J4PZH8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000564</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000011">
        <property type="protein sequence ID" value="KAF2078093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZH8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035323480" evidence="1">
        <location>
            <begin position="31"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="317" mass="34930" checksum="5688AE938CAB7363" modified="2022-05-25" version="1">MVTINSISSATMKLLNFICALFICISVVKATYPQNFYYYNSSGIFISEGENSDNPTLVPVSSSYRQLGAITKVDSQNLYAIVISDWNSVDYISIDLSSGETNTIQQIVYSNDITTGPLWGYSLDLKKNVLFSLSPTTAFHIELLINDLETQNQTVLSFGPNGVSGEVASSYDQSTGMYYAMGPYLQQFRLYVCDTNALSCKNSIINGLNYTSQKYDIAAFNGQLFVFSTDNGNVLVHTLNIDTNTLSLRDTIALDDGNVVFVQSKYNFDENNLMVLGTTNGANALTFINIINTNPVEVEGGFSIDQLITSYSLIYVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZV3</accession>
    <name>A0A8J4PZV3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TPR repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_001643</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000040">
        <property type="protein sequence ID" value="KAF2077076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="17009at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00102">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051191">
        <property type="entry name" value="DCAF12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025139">
        <property type="entry name" value="DUF4062"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19860">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19860:SF24">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN DDB_G0287407"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13271">
        <property type="entry name" value="DUF4062"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="IPT/TIG" evidence="4">
        <location>
            <begin position="79"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4062" evidence="6">
        <location>
            <begin position="289"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Orc1-like AAA ATPase" evidence="5">
        <location>
            <begin position="543"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="1032"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="1160"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="247"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1324"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1374"/>
            <end position="1438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="247"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="258"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1335"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2077076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1503" mass="171546" checksum="1630D7A5E6FE136E" modified="2022-05-25" version="1" fragment="single">FIEEKQYPVSNALKQEIQIVDINSIWKQLERFALKVKGSSLPGVTIALQNNTTIKPRATVSNALTTVNQPQQLQDVSDPLISMLVPPSGPNSGGIQVSVFGVGFAPGPSFRIKFGDILATNYEFHSNTAVLVTIPSGISVPPGQVAVYASNDGKTFGFPIQFQFYDSHVYKIPTPREQDGVVLRCQLDNLKRAIANIQTMESILLRRLSTITDDKQMLSQLYMTTNFPESGISSAFSPEYLEGADNSSSAYGSSIQSDVEDDDDEDDIADINDATSSTPKDEFDDREIRIFISSPFKDMQLDRDQIVKVVIPKIRKLCIERDIIMTYVDLRWGVTSSQSEMAISLSMCLKELERSNLMIGLFGERYGWSTQEKSDPKSQQLLAQTLEKAASDFPWVNKYKDCSMTEIEYRMILNNHSSNKNAMFYFRDPYYIEETSSKERNNFVSEGQRSKEKLDKLKLEISKSTVFKSAEYRRPTNLADVLYEDLEKYIDKKYPSSSELGPFEKERFLHNVFSKNMTKLYIQNENYNLSIDTFLTKTKSVPLMVHGDSGMGKSALLANWVKQHKEQHPEDLIVTHWVGSSPSSNKHTSTLIRIMKEIRYLIEKENTTKSKDSLFASRSHLSWIPEIPEESASSEKISADFNQFLHDVMTHPSISSRRLVIILDGLEKLDKRDSCEDLIWFPRSLPLNVKFLVSTTTASRQFEVLTKRTAEPLAIQGLTSAERKSMVRLYLQKFGKKVNDQQEIVIATSGSTLNPRFLRLLLDDICIFGEYERLNERINKLLKAKNISELYEIILERIETDYDPKGKGLVREFLSYVWGGRRGIEMDMLSALLSKKGIDPSEWNSLLVLMEDYVSSWSGMISFLNNDIAKAVEKKYITSDRIRTDIHADIAEAFTKEGDLTERKIEELPYQLIHSSKWEDLKNVLCNLYMFDRLYTPNNKSDLFHYWNVLEKQTKPPKNAAERDDPIPYSCSDEYRSLGSRSFIQASGLVISDLWFRIGSFLEEMSQFEGAEAIYTKCRGLYNNNSQNVEAAKVDRAMGRMYHTMGKFEKSESTFKQALAIFTKEKGQEDLDVVLTLNLLGTCATSRRQYAEAKKMLNQAMAICESKYESNSVLIADIAYSLGSVHFVEEHRKLDVAEEYFVRALELTEENLGDMDVSYARILNRLGSLYIEKDQFNDAEPFFKAALKIYEARFGLDHSRVSQILRQLISLYEMQEKYDLAIQCATRAISISKKIYGNTHNLVTNTQVRLALLYNSKGMKPQANSILNEVKALKEKEFGANHEQVKTIEKVLSEVNPPAVIPKPPPPPVFKPVVEELKRVLPPQPLLGKTAHPPGFIPPPPPPPPIMRPELNAYRPSLAQLMKNSIKNNPILPQQQQMQQPMQQQQQQQQRQNQQPQLPQMQQQQQQMQRQPQQQQMQQPQQQQRQNQQPQQMQLPQQQQNGYVGDILAQYDMSNLKRVQTSDRSNAVDAVNNLIGAKLSKKPMVMRKCMNEAKSMDLNSLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZW1</accession>
    <name>A0A8J4PZW1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4166 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003345</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000103">
        <property type="protein sequence ID" value="KAF2075369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZW1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025311">
        <property type="entry name" value="DUF4166"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13761">
        <property type="entry name" value="DUF4166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4166" evidence="1">
        <location>
            <begin position="19"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="197" mass="22552" checksum="2D0A6DB0E9ADAF87" modified="2022-05-25" version="1">MSVYRNVFAIAMGNRYNGLPKVIQEFHNSHHQVWNGQTLVNGSSNILVSTMRFILGMPQPTGQNTIPISLVVTFNDKGEQTWKRKFAGKKFYSTLGNCQDNNNNNNDKSSLIYEKFGLLTQYFRLIHHVDPYERLSMEFVYCCILGIKLPKFLEPTIVSNTSINPNTGLYQFNVKVDLPIVGNLISYQGYLNKPKYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZZ0</accession>
    <name>A0A8J4PZZ0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002579</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000072">
        <property type="protein sequence ID" value="KAF2076117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZZ0"/>
    <dbReference type="OrthoDB" id="18850at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31630:SF6">
        <property type="entry name" value="PHYTANOYL-COA DIOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31630">
        <property type="entry name" value="PHYTANOYL-COA DIOXYGENASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="406" mass="47796" checksum="946CDBF685C00F14" modified="2022-05-25" version="1">MNVDHNHNKLIVESEFKEDPDYCIPKYEIQFDPEGYTLSFSIEQQSEYLNFFKTFGFVIIRDVLNKKECQSTIDDIWNYIQGKSNSIKCENPKSWDEWPVITSPIFTRQALMNRSNINIYNIYSFLLNRYDLYINHERYSFVRPTKNVPHPYPLITPHLKNINQITNTATTTTTTTTTTTTVEEKENVMFLDYHHWKTEKNLQLDMNPFKYIEEKDDTKRQHFFSKLDYKQHLDFFKENNEPGNLLLNESHIHGLINLTDNNYTDDGGLILVPGSSNHLVEWAKNNLKLKQHQEEYEEKDIGNNKILRIPENNALYNQTIRVTARAGSLILWDTKTIYGYESSNTSRPHLTQSLKMFHSDLLPKQQLEYRSKQIKQLIETNDSDSVSFISNLSDLQKKVLGLNLWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q066</accession>
    <name>A0A8J4Q066_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CoA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000095">
        <property type="protein sequence ID" value="KAF2075552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q066"/>
    <dbReference type="OrthoDB" id="5138418at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33303:SF2">
        <property type="entry name" value="COA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33303">
        <property type="entry name" value="CYTOPLASMIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA-binding" evidence="1">
        <location>
            <begin position="10"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="142" mass="15642" checksum="5A16AB5531E2A657" modified="2022-05-25" version="1">MANAEIINILKTVQRIALVGASKDVSKPSNKVMKYLLTQGYDVTPVNPVLKDQELFGKKVYGTLGDIPHKVDMVDVFRNSEAALEVTKEAIDIKANVIWYQLGVINKQAEDLATKAGLKVVVNRCPKIEIHKLGLEKPTSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0E2</accession>
    <name>A0A8J4Q0E2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000001">
        <property type="protein sequence ID" value="KAF2078633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0E2"/>
    <dbReference type="OrthoDB" id="16434at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032053">
        <property type="entry name" value="Ribosomal_mS34"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28589">
        <property type="entry name" value="28S RIBOSOMAL PROTEIN S34, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28589:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16053">
        <property type="entry name" value="MRP-S34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2078633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="123" mass="14043" checksum="8BACAB4A35C44737" modified="2022-05-25" version="1">MSTNTTKFISALFSNVAKKKLKGDNFLDLCFSLPNRGVGHKITKKGWPEKESYWTVTKVSFGKTGTDFGLRGKVHGVLTWGGKTDNRIRQIPNKFEKNWTPYPYIKAVESQESKVEQTQEEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0G4</accession>
    <name>A0A8J4Q0G4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002274</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000061">
        <property type="protein sequence ID" value="KAF2076417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0G4"/>
    <dbReference type="OrthoDB" id="151517at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052050">
        <property type="entry name" value="SecEffector_AnkRepeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586:SF3">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13606">
        <property type="entry name" value="Ank_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="722" mass="84057" checksum="B3492D8FD6E341EA" modified="2022-05-25" version="1">MKKTIKLIDILYKKIFQNKYLRDCIYKYIRPHSQPRYNDVEWLKEKLLLDKDEYGSLCINNKSASFISSLEITLFKQVFYKYQDSFPKGYPPITKWAAHYNNLPVLEHLIELGYTPDEATMDNACRVGNLEMVQFLYRKADMREIDFLNLESAVMSGNLDLVVFLMGILQTFPMQQKIRLLEKSAALLHADIFRYLNARFEYSVPVSRDTFATIRNREILELCFPAQVITTFSNTALQELCICLMKGNHIDCINYLLSCPSDTVEQEIRKSVILLSLHLGKLDLFQMFDKADVDLSFITLAQIHGCLLTTEQELCYFIEKRKVVVDSIACLHFVQDEYQNLCRYLFNLPEYLIVGKNEIVCKNTKTDISIQLIRKAITTNSILIFETIKSKGFIKPNGDIDTIVDWSEFGKSNHYIKIFEIISNMLSPTQNELLKILEKSASTQSFDNLEIFKIVYQKLSNTEINNILNLACENNRIDIIKYLLSAGCRVDGLELPFGTTSNYEIIKLLFNNGCKRITNNIKLMSCVKKGYVNNFDFYFKNIKSFGSSLNGVNLQEMLYQAISHQRLYILKYLFENFNHQFEPTTEILKQLGSCSSVAIVKYFHLNQRVLTKKPRFQISFKKAIKMGDLEIAKYLNHHCNCTFTLELFLKCVKQRHSHIMEYFKSQVDKQMILSITENQVTDYSFGNSYIFQRYLSHYKFTVMDSLSSKPSKTSLFFSTLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0L3</accession>
    <name>A0A8J4Q0L3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001112</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000025">
        <property type="protein sequence ID" value="KAF2077571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0L3"/>
    <dbReference type="OrthoDB" id="15859at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00821">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="21"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="336"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="540" mass="62938" checksum="6D9948E0BC5DC4BB" modified="2022-05-25" version="1">MVKVERKKELLKWFSFGRIKSDISYSSLMKKAGGNGKGYLDRFFVLHRNYILYFKPGKTDSRPEDEQEPQGYINLGECTLEDTKSIFKTMPLTFQISHKCGRNYLIKAKDEKIIEQFLLLIRQRIKSLIGLSISALGNTEEKMKIISDLLPRSMKLPDRVKPEGATTWICKMSAYYEAYREWSPFLNKIEQFSEICYGTTKEYVDWFGGPEGPRLSMIRCEEIVLDNWIEYIKKTFAEISTYEETRFFAEDYQDIIKHIKNMIILIDGYNMYMRQCHPTVKYVDKYLEEKQVFKQHIDQFQLVPSKSNIHFRGNGTDQPLFIEGFIKASGKIKITNNDDSHRRGSDSFSDQYTDDDEKQHHHSNTAAVVEEKELVEEWKFSTSRLTRIDNGRDEDSSLYDWEFINGHFENERLGSVLWNYKTWLWSHPSKTDYKIRFDWDPISHSFVYNQPKITTGGPISISIQPSLNKLSITKKPHPATVYADWRLSKNFILHSVVIGNKTEPLFSNLEVSGAVPLPAILIIAMIPHIKESLEDLGIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0T5</accession>
    <name>A0A8J4Q0T5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Potassium channel tetramerisation-type BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000022">
        <property type="protein sequence ID" value="KAF2077706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0T5"/>
    <dbReference type="OrthoDB" id="17470at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045068">
        <property type="entry name" value="BACURD1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING ADAPTER FOR CUL3-MEDIATED RHOA DEGRADATION PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145:SF8">
        <property type="entry name" value="RE57120P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Potassium channel tetramerisation-type BTB" evidence="1">
        <location>
            <begin position="58"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="196" mass="23237" checksum="21676890B44139FA" modified="2022-05-25" version="1">MENKNRRKHIAESINGLKSLILEDINLISQEVEHLEQRKKDLLDVKKVIDESMIPDPIILDVGGKKFKNTKSTLRKIPGSYFDVMLSGEIDIKPMIDKPNTYFIDRDPTYFSYILNYLREDGKVFINDYLRLEIRKEMLFYRLLDPKVILPKEAFRTFPQWNDSREFKLIFKASEHGFKAESFHEDCDGKEDNYNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1J1</accession>
    <name>A0A8J4Q1J1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001158</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000026">
        <property type="protein sequence ID" value="KAF2077540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1J1"/>
    <dbReference type="OrthoDB" id="2410195at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="74" mass="8573" checksum="64B30A3B1787B03D" modified="2022-05-25" version="1">MKPSSLIYIFDTILDPNNGMYKEGVWFDIKMIHYVGGKERSIREWELLAKLAHLKINITPNFPNIAIGRIILSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1S3</accession>
    <name>A0A8J4Q1S3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Rab GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001555</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000037">
        <property type="protein sequence ID" value="KAF2077170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1S3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="248"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="287"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="249"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="300"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="324" mass="37128" checksum="FBD46379534E33C4" modified="2022-05-25" version="1">MTSTQTLISTRNIIIASNEDPAQESPRRNVNKSNNNIHQLHDIQFNDELKEEIQDLENPPPDIVFQGDPKYEFSFTTVVLGYKFCGKSSILQRYCNNVFDTSYLKNTIGLEFFQKKIRWSQDCVINHNFLDAMGDERFKPMNIGYLRKANVAILVADYDSLEGTLEKAIKFKEDCIFGQEIMIVLFINKVDRQFSAEDFKRKDAYVRHVTNKHPDILKYFFTSALNGYGITEAMNFINNEMYKTREFRNSPSSLHSSVESNSSSGSNNSSSNSINKKNNNIFQIFKSSNGVDHDGKSRSNNEPISLHSDNNNNRNNNKKSICSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1W3</accession>
    <name>A0A8J4Q1W3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein Asterix</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002088</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Asterix family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000055">
        <property type="protein sequence ID" value="KAF2076602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1W3"/>
    <dbReference type="OrthoDB" id="284718at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045048">
        <property type="term" value="P:protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005351">
        <property type="entry name" value="ASTER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13193">
        <property type="entry name" value="CGI-140"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13193:SF0">
        <property type="entry name" value="PAT COMPLEX SUBUNIT ASTERIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03669">
        <property type="entry name" value="ASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2076602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="102" mass="11218" checksum="0EEFE18A03698024" modified="2022-05-25" version="1">MSLSKKESAIVHGFEMRPTKSGDGAENPGDIEYWSLLCFAFAFIGIIVKYKICLWISVVCCVAYLANMKAKDAGLRQIVSSVSLSLMGLVMAYFGPNSHMFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q222</accession>
    <name>A0A8J4Q222_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CAP-Gly domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_000885</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynactin 150 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000018">
        <property type="protein sequence ID" value="KAF2077840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2130750at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034453">
        <property type="term" value="P:microtubule anchoring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028750">
        <property type="entry name" value="CEP350/CC187"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022157">
        <property type="entry name" value="Dynactin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13958:SF3">
        <property type="entry name" value="CAP-GLY DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13958">
        <property type="entry name" value="CENTROSOME-ASSOCIATED PROTEIN 350"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12455">
        <property type="entry name" value="Dynactin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0243">Dynein</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CAP-Gly" evidence="9">
        <location>
            <begin position="31"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="80"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1304"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="382"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1130"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="106"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="130"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="172"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="251"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="295"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="311"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1328"/>
            <end position="1337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2077840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1496" mass="166344" checksum="8333AC36876E0E83" modified="2022-05-25" version="1">MAEEGKSIPVGSRASITGKPELGLGMVKYVGMTKFAAGRWVGIELDKPAGKNDGTVQGERYFDCKPSHGIFVKTNMVTLESGDGGEADDMLMPPPATPAPSGIKQPASSSIRPPSSTGIRPPSSLGKGRPSLAPTTSTPTPTSTSNTSPPTTATGSSSGIRKPTESGIKRPSLTPSQAAALTSATSTTTTTSTSPPVEEKKSTSSPPIAEKEETVSPKVSSPKQASPPTEPIEETSTSTATKIPRPSIGGISKPSTTSIPKPSVSKLKESSSSISPPTTTVPTTTTTTTTTTPTPVVPEIPKPTPTPTPQPTTTTTTTTTEPVTPIKISSSTNLIEPSIPAASPMSERKKPKIMLGEDDDEEEEEINDLLNKSISNPMSVQMNETIEKMMKTINELTEYKNNSTEEINTLQDKIKEITSAKDKASKDYEKQIQQLEKSISSKEKENDQLLKQQQKYDESIEKQLTKDKGDFEKERQSLLDQINQLNENLELLTLDKELVEEKLELKEMEYEELKEEMEMIKIEFETMKLESLEEKPRVIDESLPTDVIVLQEQNEKLKEAVVKLRDMTINDKHDITKKTKDIESLTKQFNSINEKSSKLEQELSEKLEEIVELKQALEDAEVSESLITDLTDKNLELSEELGELKGTLADLEDFRDLYAELTENQTALEKSLRQDINAKEIENLNLSGQLANSQLKLQEAEKTMAQFRDLVGRLQHKLEEMRKRDEENSEKSSSWTAIQQQLLSQNIQLQNQVIRATAMEIDHQLEKQRAKEAELHLGYVYEYLPEQGFHADNDAIKMLLLLQRILLKSELAQKYLNRVYKVEEIGTTKMLGGEQEETLTASQVGHSLQVTHILDHLNISATWLSSTLERCSSEHWLRAGRSAKDMRNQEKILDHLLSIIKNEQFGSAYATTDLEKITNKLDTILANVFGSDESLICKPEWSILLNYILNIQYYIKQIMLADLNISSMTNTTDTIFPQKEMSKVLSVCRKVVKNLVNKPHIRTATIIRDLLNKSENTCKSILSDLVSALPKMQQDPQSQRQQLVITVTAKSDLLVGQLDVAGVGIEDEYSVDGSGDDHNKTSLEKLFHQMHTQLSDVVETILAGDLDLSEQDKEALSKIQSPWIERATILKQSLNEVGQLRDTIASKEAELLENLKSIKAKEAELIEEKRKEESFDKRIKILQKAENELTEKIERDTKEREENEKKYRQAIKLYQKERGTFEQEIKSLQQKNQALLSEAQKKAAAPAPTISQDQLFLQADNIQTISLKKTIKFLRTENIKLKSEKAVKELYQIIPQSTLISLKSKSSGVTKDSSNSSPTDDNNDENDNSNNNNNINNVMGVEQSKKQQIKFNEIMDLSKQVSHMMSNVLESLATPKILDISSSNNKKSNSGASSSSNPLSFGLNEIEKQKLVTKQYERKAKQLQSKLTSFISSSNGSFGLGNYADANLSRLLQKPSILGGANNSGGNSAHFEFSKNSSLLGDKDIQLALSSQRTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2C3</accession>
    <name>A0A8J4Q2C3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001976</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000051">
        <property type="protein sequence ID" value="KAF2076719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2C3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="69" mass="7833" checksum="6CAD373B7456D793" modified="2022-05-25" version="1">MTIVENFKHLSIGQSIGESVGVKDQIQYTPNQNQLISNSHLAVESSQDDNCRAEINKIVYQKKKRCEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2E5</accession>
    <name>A0A8J4Q2E5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001196</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000027">
        <property type="protein sequence ID" value="KAF2077497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2E5"/>
    <dbReference type="OrthoDB" id="843225at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23659">
        <property type="entry name" value="USP_At3g01520-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31964">
        <property type="entry name" value="ADENINE NUCLEOTIDE ALPHA HYDROLASES-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31964:SF113">
        <property type="entry name" value="USPA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="1">
        <location>
            <begin position="2"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="124" mass="13819" checksum="CC68E7196CDCDE91" modified="2022-05-25" version="1">MTRFLVSLDGSDNSHKAFQLAQSLYKPGDIMHILTIAKPNEALHKAEDLLQKYEDLASINGMKNERVVLRSSDVREGIERAIQDYSIDILILGTRGMGPLKKIFISSVSDHCMKNACCDVIIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2E7</accession>
    <name>A0A8J4Q2E7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutathione S-transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_000184</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000003">
        <property type="protein sequence ID" value="KAF2078561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2E7"/>
    <dbReference type="OrthoDB" id="17477at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051:SF8">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE GSTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="89"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2078561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="29175" checksum="6AE18F0EF16E1893" modified="2022-05-25" version="1">MSCKLYHCPFFASSFIAQFIVDLKIQDQVEIVLFGYDKMKNDFHYEKHPQGRIPFYTEGDDFSLIESSAILLHLLDKFDTDKKLSNWVDGKQKARFYELLLFHPTTIYPAVQQVFAQTLFQPGHPAYNPQVTETNTKIWNEEIVPHLSKHLGSQKFFFGDEYSVADIILSYPLVLANDLGLLTPQHKALYDYYSRISQRPSFQEIYSEANREYLPTLEKYNESWVRKAERDKKLAELKIQKEQQQQEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3Q8</accession>
    <name>A0A8J4Q3Q8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_000396</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000007">
        <property type="protein sequence ID" value="KAF2078304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="9332038at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14212">
        <property type="entry name" value="PKc_YAK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000087">
        <property type="entry name" value="Dual specificity tyrosine-phosphorylation-regulated kinase 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000380">
        <property type="entry name" value="Serine/threonine-protein kinase ppk15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF17">
        <property type="entry name" value="HOMEODOMAIN INTERACTING PROTEIN KINASE, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="298"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="75"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="145"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="658"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="801"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="856"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1097"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1150"/>
            <end position="1382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1398"/>
            <end position="1433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="87"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="145"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="184"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="658"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="698"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="715"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="801"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="891"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="936"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="963"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="991"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1107"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1157"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1174"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1200"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1263"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1288"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1338"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1363"/>
            <end position="1377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1400"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2078304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1450" mass="164584" checksum="5A2E196E1712933A" modified="2022-05-25" version="1">MTTSSTQMDGGVGVDIDSPTATATLLSAVQQSGLSDSAAFLGNNSNNSNNSNNNNNTTTTATSMSYSSVNIMDGSNTSGINHSGGCSSDMNSSEINSNNNNISGSGNSGNSDNTTPTPTSMTATIKEDKESALCIEHKHQDKQIDISLQQQQQQQQQSQQQEQQQQQLEQQPQSQKPKQDISKSHKRNQSIGGGKHKAVLHDYSFSFYSNNNNNTTDNSTTIELNDVRHKGTIESLTKNLLRFYKKCNQDFNYISSLNPRRVLTHPAEPISNEQCDNANADYIVYVNDIIGSVNGQRYKVLDSLGQGTFGQVVKCKNCDTDELVAIKILKNKPAYFQQGQLEINTLKMLNVQFDPEDKSHILRLLDSFIHKRHLCIVFELLSANLYELIKQNMFRGLSTNLIKVFLIQILDALVVLSNANIIHCDLKPENILLQNVNSPAIKIIDFGSACFENQTIYSYIQSRHYRSPEVLIGTHYCSSIDMWSLGCISAELFLGLPLFPGNSEYNQISRIVEMFGVFNHDLLQRGKHSMKYFNRNFDSDGQPIYNLKSEEEFQRETKSTLVPSKKYFNYKTLPEIIQNYGFKKNMNQSDIEKEKQNRIIFTDFINGLLQLDPNQRWTPAQAKEHPFITGEPYVGPYIPDPSKRRHFIHSAPQSIPSNIYINSNSSNHINNNNIPQYQNHNSNIQQQQQYHSNSNSNTPILSSSNGGPTKKHNIPPFKNFPSGSSNNNNNTNPNEFKLGESYSPRSIFPNSFSNSASLSHLPLGQSPSIFGAQIPYSPYFSSSNYYGSSFGSDKQSYNNNNSNSILSTSGSNVFNNNRRGRSKSDALGFNDHSLSSSEGMELPINYQQQQQYVDFNNPYNDNIMKPPMYPNNSPQRKRSQSGFPSDYMQYNNNFNNNNNNNNNSTGPPPPPLFVPRMHLDHWSGPNRFRSHSYGEQYQQQQIQQQQQQQQQQQQQNRYFNEYQKVQQQKQQQQQFQMDSPPSHLSPPIPQSPYNLTPQMISSQDPMSPVLMSDSSLNWDPSPSEELLFAFDQLPSPANNNINSTNSNIQNLTPQLSSTSSNIKGVVPPFPTHPFKNNQQQQFIEDVNSNFNNLHFNEQQHQHHQQHQHQQQKLYYQQQQQQQKPSYGIPHFSIQGDHGTQYEDTLLGLSPFTDFRQNSNSSSNSNNMNNNNSNIFQSPPKSNYINKNSPPGKSTFYSNYQQPLQHHQPHQQPQQYQQSSSSNIHSNNNLLSSSQPFSNSAPSKFQPIQQQQQQPTQNQFIQNSPPGSFSSNKQPIKNRPRNDAFSRISPPNQRYGQQPQNMKSPPTPSKTNYNDFSSHDLNTNPLKLSSDDIQRSTKYNNNNNNNSNQQQYYPNNNFNNNQNIKSPSINSLKNNDNDINPLKLSSEDRLKHQNQMNQVFNNNNQNNNQNNNNNNNNNQNNSSNNNNNNNNSRKVFIGSYRDKDQSSPPPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4R5</accession>
    <name>A0A8J4Q4R5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">UDP-MurNAc-pentapeptide synthetase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_000068</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000001">
        <property type="protein sequence ID" value="KAF2078697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4R5"/>
    <dbReference type="OrthoDB" id="7627253at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047480">
        <property type="term" value="F:UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="entry name" value="Mur ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1390.10">
        <property type="entry name" value="MurE/MurF, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02019">
        <property type="entry name" value="MurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000713">
        <property type="entry name" value="Mur_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051046">
        <property type="entry name" value="MurCDEF_CellWall_CoF430Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035911">
        <property type="entry name" value="MurE/MurF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005863">
        <property type="entry name" value="UDP-N-AcMur_synth"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01143">
        <property type="entry name" value="murF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43024">
        <property type="entry name" value="UDP-N-ACETYLMURAMOYL-TRIPEPTIDE--D-ALANYL-D-ALANINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43024:SF1">
        <property type="entry name" value="UDP-N-ACETYLMURAMOYL-TRIPEPTIDE--D-ALANYL-D-ALANINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01225">
        <property type="entry name" value="Mur_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="MurD-like peptide ligases, peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63418">
        <property type="entry name" value="MurE/MurF N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0133">Cell shape</keyword>
    <keyword evidence="9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur ligase N-terminal catalytic" evidence="11">
        <location>
            <begin position="14"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Mur ligase central" evidence="13">
        <location>
            <begin position="94"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Mur ligase C-terminal" evidence="12">
        <location>
            <begin position="303"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2078697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="428" mass="47274" checksum="95E77DCC39C1A876" modified="2022-05-25" version="1">MEQLYQLYLQHPVVTTDSRACLPNSIFVALRGENFNGNAFAGKALEQGCAYAIVDQAEYAEAPKTILVDNCLQTLQELAKHHRGQCTGTTLIAITGSNGKTTTKELVASVLSQKYKTLFTQGNLNNAIGLPLTLLKLRNDHEFAVIEMGASGPGDIKTLCEIAQPDIGIITNVGKAHLKGFGSFEGVIKAKGELYDYIRSKKGKIFINHSNQFLQEISSGLDKFEYGTTSGLFVSGKVNSERSLYFSFDWKTNNSELQLIQTQLVGEYNLENALAAAAIGSFFNVETALINSALQNYVPKNNRSQLKKTQHNTLIIDAYNANPTSMYASLLNFKNINVSPKAVILGDMLQVGEYSKEEHQKIVDLLVECQLDKVFLIGECFSSTKNQYNTFGTLSDFIDKISEINLIGFTILIKASRGLKLERTIEYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4U9</accession>
    <name>A0A8J4Q4U9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000326</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000006">
        <property type="protein sequence ID" value="KAF2078342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4U9"/>
    <dbReference type="OrthoDB" id="1026733at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01767">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050730">
        <property type="entry name" value="UBX_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322:SF93">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="2">
        <location>
            <begin position="520"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="440"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="307"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="592" mass="65464" checksum="7AF08ED025705AD2" modified="2022-05-25" version="1">MSNNNSHRSSSSSSSSSGRRSNIRTTRDLSPSSSGGGTSFIGLSGNNRHRNHHSSSRSSSSSGNGSGSPNNNLNRHQMVVEEAVMDDGGYSRVTTQSVQTSVNPHNGSVSQVYAHSTTTTNKPTPTRTNSSNAFSVTSSSSSSSTSSSTTTTSSSLGGSGGISGGPQAGTSPGIGVGACNVNANGGGHFGNYTPFLQRDFMTNISNERNKGPLSHVRPQDIFEQAQEYRRRLQEQQGGGGGGDTSNGGYSGTRPPRAFIQDEDDDEYEDDDYEDEYDETELMKQYQQGFDDEEEALQRAIRESLLISSPKQQSNTSNNSPSNRNFHSNTPSSPTNSINSNNSNNSPSNTNKKKPVRTPSPVYNNNRKVRFGGGGGDHDNEIRESVDQYYEGESESPDEDDQYFEDNPDAQNFLTPHEKARLGNKRMIKESRRILQEQQREYQEALMRDKEREEKLKKEEREKEEKKQMEEAMALSLQMEKENALNTKKTRLPADPSLSLSPDQIKASPIKICEISISLPPDGQRITRKFYVNNTLQSIRDWIDIHLSESQSTINTDTYEILTTYPKEVLADNSKTIWDLNLYPKTLLNLREP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q503</accession>
    <name>A0A8J4Q503_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_000367</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">PML body</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000006">
        <property type="protein sequence ID" value="KAF2078384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q503"/>
    <dbReference type="OrthoDB" id="9975959at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070260">
        <property type="term" value="F:5'-tyrosyl-DNA phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09080">
        <property type="entry name" value="TDP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051547">
        <property type="entry name" value="TDP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15822">
        <property type="entry name" value="TRAF AND TNF RECEPTOR-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15822:SF4">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="12">
        <location>
            <begin position="212"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="168"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2078384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="478" mass="55335" checksum="882C86119C0DEC4F" modified="2022-05-25" version="1">MNRSEEPNIEIKDTNKICGLTPPPPPPPPPPNKQLKLSNNPNIIITSSSTSTTTTSKFVHNQQNKNNESKIDFTQQLLKFNRDQLLKTKIKIRTPDGRVFEEQLLANSGQSFCEYKGLDEYTTTKIYNQVTGLSSFSYCKNVKSWVPYIRATSEFEIQSPPPTSTEAVDQKVDEQQQQEKEEKEEILVAAIKEQVKEREKEMENNHNILTFITFNVWFDQYFWKERANHLFKIIKEKTPDIVCLQEVTPVFLDYLTSQLWVKDNYCLSDNGNLDTVYPYGVVVLFKFKTMFLESMCICPLPSTKQSRKTLCCTFKYKYNKNKSTMISIATAHFESLDINSSSRVRQFNYISDHLDSSNSNHMFIMGDFNFGTNYIENDEIPKRSFNDLWCQFNNTDDNGYTYIKKKNRVDRILFKSNNNSSFSNGTNDYSTLMPYQISLIGGDPIPINEHSRSLENIAADELIYPSDHYGLYGKFIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UNN3</accession>
    <name>A0A8J4UNN3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010482</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001142">
        <property type="protein sequence ID" value="KAF2068191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UNN3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="150" mass="17430" checksum="BE651C7278ACA6C3" modified="2022-05-25" version="1">MLSWCFATLQNRPSSMFFNRIKDQWDDLRGTALTARHRDSIIKPSKNQCIRNTNTKPLSLKEVYRIRLNLPDQIPLTYFQIERIFDFNSEVATRRSMANHFETISKIAYNKGRNTPLGSLPVPYRLITKDKSNAHMAVDTILKNPTCTHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPW4</accession>
    <name>A0A8J4UPW4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Short-chain dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008848</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000588">
        <property type="protein sequence ID" value="KAF2069826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UPW4"/>
    <dbReference type="OrthoDB" id="1933717at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="61" mass="6579" checksum="22F6E87D5738355F" modified="2022-05-25" version="1" fragment="single">LRSEGFKVNSANPGFTATDLNQHRGPKHVSQAGEFIARIASLPPGNIPTGSYFNEDGLLPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4USC4</accession>
    <name>A0A8J4USC4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_005241</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SID1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000203">
        <property type="protein sequence ID" value="KAF2073456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4USC4"/>
    <dbReference type="OrthoDB" id="416618at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051033">
        <property type="term" value="F:RNA transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025958">
        <property type="entry name" value="SID1_TM_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12185:SF14">
        <property type="entry name" value="CHOLESTEROL UPTAKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12185">
        <property type="entry name" value="SID1 TRANSMEMBRANE FAMILY MEMEBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13965">
        <property type="entry name" value="SID-1_RNA_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="84"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="186"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="210"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="265"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="379"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2073456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="413" mass="48098" checksum="42D0301A562B37C6" modified="2022-05-25" version="1">MSSLNTDANETDTLVEIPSTVEELMIQSEVQRKIKSQPVYLKYFWKILLIVSAFYSLPSIQFVFFQYSDPSIQCYFNYKCARNFAGLTAFNNVLSNIFYITAGTAFLLITHFTAHKEDGVHGLHTDMSLYYCMGLTILLEGVFSALYHVCPSKLNFQFDTTFMLIGSGLLFFTVHQKRHATFTAGAFKAFSFFALFIFFNFLSLTNINAYVFWTLFFLLFGYISIVGSAYLLAHRRLVFNNSLSVIWFYIKKLRHPSQIDDRPRFITILIANVLSWGMVLANVILGFLSNIAKDFSNLILGVIILNFLVYLFYYIYMKFKHGEKVHFGIWILFIVMLVSWTVGLWFFEIPVTNKFLTFDESLKLNEPCVVFDYFDTHDVWHFFSAVGLFSIMSIVYFIDWDLRYVPRSLIHVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UU91</accession>
    <name>A0A8J4UU91_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000928">
        <property type="protein sequence ID" value="KAF2068563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UU91"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="65" mass="7116" checksum="1D6E9C07C6222D03" modified="2022-05-25" version="1" fragment="single">MEAMDTTSAPTPTPLQERVVLCIGKTGNGKSTLCNVLSQTMDFKESPNSVSETKEHKKQEYIIDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVR8</accession>
    <name>A0A8J4UVR8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Putative zinc-finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001859</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000047">
        <property type="protein sequence ID" value="KAF2076831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1922977at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019607">
        <property type="entry name" value="Putative_zinc-finger_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039278">
        <property type="entry name" value="Red1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21563">
        <property type="entry name" value="ZINC FINGER C3H1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21563:SF3">
        <property type="entry name" value="ZINC FINGER C3H1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10650">
        <property type="entry name" value="zf-C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative zinc-finger" evidence="3">
        <location>
            <begin position="913"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="104"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="176"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="340"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="648"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="850"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1082"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1407"/>
            <end position="1426"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="462"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="54"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="122"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="148"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="208"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="260"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="340"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="378"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="414"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="649"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="690"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="708"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1095"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1126"/>
            <end position="1135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2076831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1871" mass="216094" checksum="81CD834711DBC9A6" modified="2022-05-25" version="1">MENNDGIDIVPTYILSQEQQQDEQQQDEQQQQQQQDEQLEQTHNDLVDDKIVEPSTTTATPTPTSTTATTNARAKLLPKGAYNSESMNKLQSLKQKILETKGRLRNKQLEDAEDGEILDEPSTNNSNSNTTTSDPKQQTSPKQQQQHLKPHKGKKNKKGNDRYPQIYEQVEDMDISEDFNNNNNNNSLLQPPPTSSSFNYQRNNHFQQPPPPPPPNFNPHHISALQPPAPTPLHYTLQLQQQPPPPMNMMNGYNNSFDHHHQHQHHHHQHHHLQQQPPPPPNYNGYNMVSNNTSQLDLLLNQISSQTPNPVVSSMLQIVKTQQLQIASFQQQVQKLQQLHSNNNNNNNNNNYATPYQPQQQQPQQILDDEEGNIDSGSSSNSTTTTTTTTTTTATTTPTPSTNNNISSNNVTPQFKNLYNSNNGIVSDPSLVINPEDHSDSDSDSDDSDHGISTDDMIQSLAEKQMTELERLKKEIAVKEEANLRKKLEYLKSMKLQKEQQQLQEQQQQQQQQETYRQATIVDVSTKSINSNTRTSKQVNSATANTNDVDGVRNQVMKLSVDILKCSQELNKNRSSIKSIENELGTLNTKKGELTNELLQVDSLIQENSQKLLQEKNHSMSLSDQHSELLEILNQKKALLKQLEEYSLNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNKSSASIDQQQKKRALDNDNSHSRESSPNKLSPIDSSTSSVIKNKKTKVDQLKEKLDISRVQLSEQLLRSQLLQANKKKLLLSKTNGETSIDQTMMIENDPIVETFKNNKNNNNNNSISQEIQDFMLSIENTSLKPSIPLMIDFIDCQHPQVFQKIDPVQSNKLLLLERGNNTSSSGSGSNSNSNSSTTTSKYISPLISLRSYRFSPNYSVLVGNEYLSSDNYSIKLNPFKKFCMYELDGICRDNECTYQHQRDYKQTPHDIIKDIISFYSLDSNTSSEILKSCLSCIGSIPIDQIISNIMEIIKRKSNNNNSSKQQQQDESNLFLDIPKERKELLFNRLKSYEDQVLLLQKIQYYQQRTIKTENDINKPPVARKEQNLTKKLKKVVEQQDDENFIHLTKTKEVLTKPKQRQLKNNNSNNNSVEELDQQQPQPTQQQEENEEWNIDDITINNNIDIGDKEEDDELDKDSMKKRYGGTSFISQQEYHSDPSNLQLCYSYLGSNCGSTTTSDISKDEKNRNQILLALCDIVKYNTQSMLFWKAYLEIFILKFENSSKEIQELISKAIQSTSNNSSIIFHMMRIKLITNTLSQKVDLIINTLDDIFNGVLVFKNNLEKSFWLFTFILLYISNEIEAGYRESILIDLFKFFNIQVMAGDVDDQDNNINNNSNSNNLSSKSFLFKYKESLIKYLEPREIQNLKLTLLSIYIFNQVPSITCFYQNIYSLNDNNNSNNNNDNDHKQQQQQQQDDSSINLVSRPFLLDFNSFFNNKSYLTNDENENTILKFLFESLLDLGNGDNFDQQQQSTTLYFNYIYFTNSFNDIFNSFIFKQVNDNSIYSFLILLSEKLNVSIPMDIINNGNHNSFELMIKLVQTLIQDKSKCIKLIESFIKSLYSNNGNDLDVQILFDILIKRNDSIYSLEYNQLPHIDVKNQKVYIWILYSVYIYCIYDSIDEAFKLLKESLKTLKLVDRKLIWIELFKLIEKTNTIDIDQLIDEFFIDVPTEFQYPFSSYTESIPYDIPPFLSTVLKPIVSKDYSTHNLILDIYFTFKNDHRPLIKSFALKFPDNPFIFKKYIEIVQWTNNFEYIKSILKSIKPKRNIPLSSWKLAIQAGKDFNIDPQQISKLEKNALSFHPFNYEISLDWLKNWMKEDKDFLIDDDEKLLIILEKLCYFGINLKFPQHIFDIVNLKYKSMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWM5</accession>
    <name>A0A8J4UWM5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Centrosomin N-terminal motif 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_008959</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000607">
        <property type="protein sequence ID" value="KAF2069718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWM5"/>
    <dbReference type="OrthoDB" id="21683at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012943">
        <property type="entry name" value="Cnn_1N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07989">
        <property type="entry name" value="Cnn_1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Centrosomin N-terminal motif 1" evidence="5">
        <location>
            <begin position="157"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="221"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="325"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="415"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="546"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="78"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2069718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="595" mass="68118" checksum="EA7E5826C4F0DA32" modified="2022-05-25" version="1" fragment="single">MPPNSTTGGDDLADIEDDSDFDFNPETPLKNRFGKSATTTTTTTTTTANRGLNGLSSASTSTSTSPPLFSYRSSPPMMNGVNSNGNYNYQQQPSSSSSSSMSSYKGSSMSSINNNNNSNSSFRINRMNGSATDLTSSVSIDDMLPTPRSADNNDSNLKELSTQLEAYKKENFDLKMRLFYMQENVNRVTTSQDFDIMSQNVDLQVKLSEKITELEEKDRMVLKLGSAIDQSKAKIEKLKKNQAGLEDTIRKQKDDIDALNDDIAQKQQHIELQQQQLVDQRLLAQQPPVIYEQVPVVSAPSTRVVTQYINDPKDMETINQQDHLINDLKLKNNDCLRQIQDLNRQVAEYRNHDKYIKQLEDNIKELKQKEEKLLDTIDQLKQDLLAEQMKPLPHQDNSAVYEKIRKLQELHIKDLEKQKELQNKLDSALKKLEALKEHYESLRGNYELLLDRYNQAKTQLEAATLRIIELESQLEQKDLDFSALQHDIDQLRHQHQLAMDQADVNHLNELNSKAHLITELRNQIKRIRIDNQISITNEQETSVQMLHTFEESINGFQHDIQELENQLILTQNDKQDLEAQITHALAEKDQLAQER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWW0</accession>
    <name>A0A8J4UWW0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_000742</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000015">
        <property type="protein sequence ID" value="KAF2077941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWW0"/>
    <dbReference type="OrthoDB" id="21026at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052410">
        <property type="entry name" value="DRC5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24107:SF2">
        <property type="entry name" value="NLR FAMILY CARD DOMAIN CONTAINING 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24107">
        <property type="entry name" value="YNEIN REGULATORY COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="383" mass="44664" checksum="45707E3617D31DD7" modified="2022-05-25" version="1">MGNTSSKNKFLQNLIQIDVDNGRRKFTENPDVLEKDGASIIENNDEDNQIIVQSKKKYKKDKKNNNYNNNDNSQMNLIHVQDRIVLISNNDTNHNNQLTINHMPESLIYKIIKYAVGDIFPSKHFFKISRVSRLFCKLSLKSIKNLFYDFPYLYETNKHVNHCKIVSRFLAIDNHYFVKNNIQNARLGFGLDSVGFCLMYPQLMECKTLRSLRILFYHSIVPKERIKSVLNILIENHNIRKLNISQDVVHEKTCKYISMVIGTDLTNIKELVYQKAKINSEKFELLMKGCTRSTTLKFLDFSKNVISDENLDLITHYLENSKTLEYLDLSNNQISDVGCHLIIQACKKSKSIKKLNLSGNLVPNDQIESFNIEYSKHFVYFRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXA9</accession>
    <name>A0A8J4UXA9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">serine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Seryl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_008439</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="1"/>
    <dbReference type="EMBL" id="AJWJ01000520">
        <property type="protein sequence ID" value="KAF2070243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXA9"/>
    <dbReference type="OrthoDB" id="10264585at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00770">
        <property type="entry name" value="SerRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000055">
        <property type="entry name" value="Serine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="entry name" value="Serine-tRNA synthetase, tRNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033729">
        <property type="entry name" value="SerRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="10">
        <location>
            <begin position="229"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="311"/>
            <end position="313"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="326"/>
            <end position="329"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="397"/>
            <end position="400"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for serine binding" evidence="8">
        <location>
            <position position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2070243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="479" mass="54372" checksum="B91F38BA7659D1A3" modified="2022-05-25" version="1">MYRIGSRIITNTRPQQWGYLNYTTKNNTTTTATKQLMNDETHVLQHIKPQLNFDYIKDNCQQIQDNAKKRNSSVDVHNIVDLYQQYRVVKKETDTLRAKRNIVAANMKGVAKGDTKERNRLIEEGKFIKEQVNICENRFEKLHNQLYSEALKIPNNTHPDVPIGPESNARVLEMSGEKPTHLQEFAFKDHLELGESLGVIKSAAQTTGHKFYYLTREGAMLEMALSFWAFSKMTNQHNFIPVITPDLVHPSLAEACGFQPRSEATQLFWIKDQDLCLTATSEIPLAGMYSNSQIKGSELPIKMVGYSHCFRAETGHGVHNKGIYRVHQFSKVEMFVVSTPEQSDEILDSLLKIQIEMFKELGLHFRVLDMPSEDLGAPAYRKYDIEVWIPSRNDYGEISSASNCTDYQSRRLNMKYTATNGGPSAFVHTLNGTACAIPRLILAILENNQQPDGSVLIPKVLQPFMGNKERILPLNGSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXB7</accession>
    <name>A0A8J4UXB7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nucleoside diphosphate kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_000288</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the NDK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000005">
        <property type="protein sequence ID" value="KAF2078421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXB7"/>
    <dbReference type="OrthoDB" id="25346at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46161">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46161:SF3">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE DDB_G0292928-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside diphosphate kinase-like" evidence="8">
        <location>
            <begin position="4"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="6">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2078421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="148" mass="17163" checksum="4C76C17D9306805C" modified="2022-05-25" version="1">MSLTRYTLAIIKPDILEKQSKSIEKILSTIQEKFIIHEKKQLQLTIEQAEQFYSDHRGKFFYERLVCFMSRGPIIPLVLSEKNVNSSSSIKPWRDFIGPTHRDKAREINCLRGQFGTSDTKNAFHGSGSEKEAVDEIKFFFPDFKSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXH8</accession>
    <name>A0A8J4UXH8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Adenylate kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_007723</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the adenylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000428">
        <property type="protein sequence ID" value="KAF2070950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXH8"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019205">
        <property type="term" value="F:nucleobase-containing compound kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2070950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="537" mass="62086" checksum="82F850B0A383B5B3" modified="2022-05-25" version="1">MISKNIVMSSLMKRGFSIPQKAYYGTLCINTLNNNNNNNIYKINNNSTISNNINYYFDHQHHYHHNHHQSSLSLLKRYYSVNSSNSNNEDDLIFQKLEKERQDEQQAIENQEQEDQDLDHPNLLFQQHVNDTTSSSTTIIDDDLEIKDPAIIFNTVWNKLEQKYGKENMRFPQEIILVAGAPGSGKGVNTPFISSVRGITGAPIVMSSLLDSSEAREIKSQGGMVSDSFAFQTILEELRKVKYRSGVVVDGFPRTTTQVELTNALYEKMKELRRTFFKTPLASCFPRPIFRVTVLFVEEKESVERQLKRGQITRETNIKLKEQGLPLLEERDTDCSEEAARKRYKIFRDHYPTLQTLKKHFPFSIIDASKTLDRVQQSIIREFKYQSSLELSQETYDLVQRIPLVTDITLHARVDLVTRLDDYQNRHTDLFTSVVHVIEKEFVPTIRRHSIAGSAMIRVTNSLFSSALAIDIALDVLSERGFRATVDVKTTYIPHKINPTTYEIESITQTEYQFKVDFEKPLIREEYSNITTTHMMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UY95</accession>
    <name>A0A8J4UY95_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_006330</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000287">
        <property type="protein sequence ID" value="KAF2072360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UY95"/>
    <dbReference type="OrthoDB" id="28875at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15845">
        <property type="entry name" value="SNARE_syntaxin16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF83">
        <property type="entry name" value="SYNTAXIN-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="291"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="218"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2072360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="314" mass="36558" checksum="B3FD095BD86CEA24" modified="2022-05-25" version="1">MATRDRTPAFEKIKAEYKNRRPTRPVVGGSNFYEPDIDHHDNNIPLVERKNKDANNIQLKSLNVLPAWMQRVNDIDSNLTKIKTQIEKLSELHAKHVLPTFDGESQSDLERNIEILTSEITRLFHVTHQIIKTLGSDAKLSQEDTKMKKNIQTSKSAKLQNLSLTFRQKQRTYLSNLQRSSNSFSDWDQNYDNKEEELIEDIDLDFTMEQKKYVNVLEFEVNARDQEIRKTLASIQELSHLFQDISLLVVQQGSLLDQIEYNLDQTEQFVHDGGVEVEKTNQYHKEYRTRLCILLVLVALIVAMIFIMIIKAII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYV8</accession>
    <name>A0A8J4UYV8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chitin-binding type-4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_005015</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000190">
        <property type="protein sequence ID" value="KAF2073665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYV8"/>
    <dbReference type="OrthoDB" id="17911at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.50">
        <property type="entry name" value="chitin-binding protein cbp21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004302">
        <property type="entry name" value="Cellulose/chitin-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051024">
        <property type="entry name" value="GlcNAc_Chitin_IntDeg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34823:SF1">
        <property type="entry name" value="CHITIN-BINDING TYPE-4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34823">
        <property type="entry name" value="GLCNAC-BINDING PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03067">
        <property type="entry name" value="LPMO_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035258233" description="Chitin-binding type-4 domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-4" evidence="3">
        <location>
            <begin position="28"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2073665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="246" mass="26626" checksum="C9553C7D3958402D" modified="2022-05-25" version="1">MNKLFSLVFLIVCVSFTNGHGYGVGSLMSRQIKCTTSPSFSIWWPEDGSGIGDLGCKNAFQYVKAKTGSADAARYQFVQKNEYSVLIPNYAAGYSALTAAVPKSLCSAGAVSAGGQFGDKSGMSIPNTWLLNKITVPSTSASSKNVTFEFCATAAHNPSYWEFYVTKSGFNIKTQNLTWNDLTLIQSTPNTPAVTNTAANCESSKAYKIPITLPARYQGATLLVRWQRIDSVGECFINCSDFIFSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYW1</accession>
    <name>A0A8J4UYW1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ribose-phosphate diphosphokinase</fullName>
            <ecNumber evidence="4">2.7.6.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_005030</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000190">
        <property type="protein sequence ID" value="KAF2073680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYW1"/>
    <dbReference type="OrthoDB" id="413572at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000001">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002320">
        <property type="entry name" value="PRK01259.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF89">
        <property type="entry name" value="RIBOSE-PHOSPHATE PYROPHOSPHOKINASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="13">
        <location>
            <begin position="4"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2073680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="316" mass="34539" checksum="FA8AF353E1DDFF53" modified="2022-05-25" version="1">MESVKILSGTAHPEFAELISRNLNIELGKTHCGKFSNGETSVVIGESIRSKEVYIIQPTCSPNVNDNIMELLVLVDAVKRASSKSITAVIPFFGYGKQNKKEKSREPIVSKLIANMIEKSGVNRVISMDLEASQIQGFFNIPVDNLYAEPLIVKYIEKHIKGDRVVVSPAVDGVKRTKLVSDKLNCDLAILDRTTKEEKNDMILVGDVKDKIAVIIGSTADTCETLALSAKVLKRSGATKVYALVSHGELSGNAIDTLNNSELEELVITNSIPIGEKTKQCPKIKVINVAPMFSEAIRRIIHGESMTAQSSKFTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYZ5</accession>
    <name>A0A8J4UYZ5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_004873</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000181">
        <property type="protein sequence ID" value="KAF2073815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYZ5"/>
    <dbReference type="OrthoDB" id="1919336at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050140">
        <property type="entry name" value="SRY-related_HMG-box_TF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270:SF161">
        <property type="entry name" value="SEX-DETERMINING REGION Y PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270">
        <property type="entry name" value="SOX TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="HMG box" evidence="5">
        <location>
            <begin position="110"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="3">
        <location>
            <begin position="110"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="96"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2073815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="173" mass="20071" checksum="BE5FB59DA0A4E49B" modified="2022-05-25" version="1">MNQQPQQQQQQQQQDGSQILPLLNTFKLPDSLTNPFENESNEDDSDFDNQSDLDDLDESDNNNNNDSDQEDEHDDEDDEEQDEEEQQPVTTTTKVAKTRGKTAAKDKDRKKKPPNAFILFSQQRRKELKLNNPDLSNATISTLLGKEWMELHPADKKKFMEESTKIKKQCEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZ53</accession>
    <name>A0A8J4UZ53_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004639</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000167">
        <property type="protein sequence ID" value="KAF2074070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZ53"/>
    <dbReference type="OrthoDB" id="590761at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF18">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E HOMOLOGOUS PROTEIN, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="458" mass="52305" checksum="8EA384674C4126DF" modified="2022-05-25" version="1">MDTTSFIETDGQTEYSHVSYPDQEMPSSNQDQTICFKSNGLRMALQNIMSDGKGYILSGFEIADNVDLEEEMEYNPTKDSPQKFAENAEAKMNLEAKFRSHLDTNHIKAETITKLLTSISNSPMTFIFKPSLIPAQIPATTTTTSTTSTTTHTSPTPNKIIPARASNFINSDSQIQTNQMVHHLSNTYYFYYMPKRPKNMNDYLTGLQFISGPIKTVEDFWSFYSHMIRPVDEFSSLSDIYLFKEGVKPIWEEKDNKKGGKFVVKTRRNYTSKWWEDLLLAFIGDQIDGCENINGVVISFRANDNNLIGIWNNNCEDIERLSLSLKQLFKIEKLDYKPHFNKLDRIFKDGQLGGSEGSEGTTLNSSIDSGMDYFRYQPSLNSSGNSINGGNIKLNTSGSGISLSNNSITSPLNNFMQQQNIQDYYQQMQQQTQQHYMQMQQQQQQQQQQQQQNQQLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0K6</accession>
    <name>A0A8J4V0K6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009329</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000681">
        <property type="protein sequence ID" value="KAF2069347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0K6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="335"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="472"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="625"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="99"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="143"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="198"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="247"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="495"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="630"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="660"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="709"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="721" mass="79917" checksum="C69BEA9B4AFBF368" modified="2022-05-25" version="1" fragment="single">MGDIILNVFGLVDKRLNRLKGLRVRTKSDITSVLPQYAKIQEILSNAKKLLEKDPDNPQVIQQNLNQIEIRIKLTTQETDTLHARLANNDYVETVPQYSNNSSTDSSANSSVNSSPVYYKEQRGTFGIIFKTANSEYTTIKPSSSNNNNNNSSNSNSNSNSVDIDNEENIKLQKLEEEEDRELEIKFKPMTTLDLGESSNTNSNSTKSSNTNHSDKVSPRPPLFGEITLGNSRGNSPRNHNGKHRSGSFNTFTTPSNSSSNSNASHSTDSPTKPPSHMSNSFNLYENNQISVQQKREQITKMLQDQHFGKAPTSYNPKDISFSYKDDVLKNIKQRSSIDDTTNNSNDIGAGGKKRANTTVPKPPGGGSFIRKNATKTETSKAEDNGQDEQPQQQTPKDNIKDDKQQEIKVEELKVEQDTVVLEQIVDEARVEQQQQQQQQKVEEVVKIEEIQDKLNETVICNTTTTTTITTTAVNDQDSEDENTVVENNTPDTSDMSEDSEEELTTTVPTQTTTTTMEQVVEIKKVKVIKDINNNNSSLVIDKNSNNTTTTTTTTTTANNLNIIEREEQQLNSNNNMISTSLVDTFLLRNKMESDLSISQFIQPYINSSDSIILSSSTPSISMFKQSYPSSNGSNSNSNGSSGGSLNGSNSNISAPTIGLKSSQQKISQKLKLSKHTPMRKQSEARIGHARSNSHNSHSKLRTTTTTTSLEPNNDFISEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0S4</accession>
    <name>A0A8J4V0S4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Battenin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002523</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Lysosome membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the battenin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000070">
        <property type="protein sequence ID" value="KAF2076170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0S4"/>
    <dbReference type="OrthoDB" id="5965864at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003492">
        <property type="entry name" value="Battenin_disease_Cln3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10981">
        <property type="entry name" value="BATTENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10981:SF7">
        <property type="entry name" value="BATTENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02487">
        <property type="entry name" value="CLN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01315">
        <property type="entry name" value="BATTENIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0458">Lysosome</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="339"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="377"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2076170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="406" mass="44952" checksum="CE4DC599DB80BEFF" modified="2022-05-25" version="1">MARNALFIRNWISFHFMGNINNFSYCVVNAAGQSLARYFNNENNIGLILWANIAFGFAARLLNTFVLEKVGPKLKITVNSVIMAAGLIGVALSVYINFAFCIFSIALIGISSSFGESVILSHMKQFPSDLVNGWSSGTGVAGVSGTLFYIGMVTAGLSNSKIFYIILPTVGIYWLLFFFGLKVPGLPGTEDNTKTGKGDKEGLLKKDQLAENDTSVAVEDDQPGVEPMYGPPGETKKQRYIRCAKLVWWNALNLSLVYFFEYVASVGGADKAINSTDSQDWFVKNFYAIISFCYQLGVLCSRSSLQFIKIKRIEVLTVLQGANMVFWILQAQYKFIGSVWPLFILMIYCGLLGGASYVNAFYLILHDKKIPNEDRELCINYAALLVTVGITSASCFILIMDHTFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1D0</accession>
    <name>A0A8J4V1D0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000501">
        <property type="protein sequence ID" value="KAF2070367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1D0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="192"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="280" mass="31898" checksum="A7E7C8A14E0933D5" modified="2022-05-25" version="1" fragment="single">MELTKSSMRETDGGGRFFISTSINSSSSPHNNHHHHICNNSHSHYSFNHNHHHPIEINFLPSNFGNSTTTTKTISSSSTTEYLSSKKRSRENIVYTSSSCNGSSHHHHHHHQTECEEYEESEEEEELVFDSYIENDNDDHNSLSIDKELLENSNSNSSRRSSISSSSKHLLEYHYVNSNNNNNNNIHKSRDNIVNNNNNKNSINESPIKIQSTPHLHHQSGFSPSTLTVRTFSPSLSLLDQQQQQSDLCNNNHNNNNNNNNNNNNNNNNNNNNNNNNNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1G8</accession>
    <name>A0A8J4V1G8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein phosphatase 1 regulatory subunit 21 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008222</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000489">
        <property type="protein sequence ID" value="KAF2070457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1G8"/>
    <dbReference type="OrthoDB" id="19247at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040024">
        <property type="entry name" value="PPP1R21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049372">
        <property type="entry name" value="PPP1R21_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019343">
        <property type="entry name" value="PPP1R21_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21448:SF0">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21448">
        <property type="entry name" value="SMOOTH MUSCLE MYOSIN HEAVY CHAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10205">
        <property type="entry name" value="KLRAQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21636">
        <property type="entry name" value="PPP1R21_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01254">
        <property type="entry name" value="KLRAQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein phosphatase 1 regulatory subunit 21 N-terminal" evidence="3">
        <location>
            <begin position="65"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="498"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="547"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="424"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="507"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="547"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="569"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="998" mass="114513" checksum="4F0CC4400E940313" modified="2022-05-25" version="1">METNNNNNNGVGGGGSVGSNLNSLLSSSTGSFGSLLSPIQSGLSGGNNSNNNNNNNSAEYDSKVQKIVANYQKIKGQNAILKKALVQEQQKTTDLEKELKEKSQALTTAIQEHDVLEFNNQRLTKRITLIQEANTEKKESGWGFGKSKVELQKKEEEINILKSELQEKIDDNESLHSVTTEKENAYKALDSQLRGVISEKDSLLVEKDLKIESLLIEQKESEKKLVDQTRGLRENIGLLEKSLKQVYITAKQRDDALQQLSQIFENGLSNQPPINFDYPIENITPSNERFSFDDIIFDKTFQDITLNSASFNSIADKNQWSQPYRAIIICVQNFISYHLDIISKLTSSHQSIVKSLNEKHQNEIIDLNENKSIITLQKEEIEFTLNSKTEELTLKEKEVLLLSQELLEKTQLLNQQLQTSRVIDEQIQNERAEYQKTKSQLEQQIITITNNFQDELKKAKDQHQGLLRHQKQLLNSKIEQQNSIILNLQKQLSSLSVLQPTNSNSGSSNSLIDLDTSNNSNNNSNSNNTSFLDLSFIEGQLNNININNNSNNNNNANTGGSSLLDFDEQQQQQQPPPATTATTIINSAEFNSTDSTSPKVQSERFKLTVLDDTGDQLESLNFSITDKEREKEMKIYYENKITQLLNKISNIDAKTVRYHDQYQQLLKKGSGNDNVKKEHEATLLELKNTKDELEVTRFNYDQQLRMMTEQFVSLNESISQLDSELLNIKQHKILCAKCKTWNPLEFIFSPENPNLYCSKGHPLQTLQIIKIPFKLFDFNPNTKKFAKKKSTDNLYGSVYKLKRNNLNWFEIGCKQLHVPIDRYNVYWKIQIKEYTKPLYFHCSITIDKEIYTFRQKVIERETILANIGEWRLFKTNIIAPPPNSIKGNTHMVVKCMLYNIDQFSTTRLSNIVIDYFQLVPIYEELPTTTTPPIVYIDTIPDFPSPSLYEPIDCQHFIPIEQDQDSALYQNQSKLLLQRCSERRKEMIVLFNKENYFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1H1</accession>
    <name>A0A8J4V1H1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NFACT RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003244</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000099">
        <property type="protein sequence ID" value="KAF2075463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1H1"/>
    <dbReference type="OrthoDB" id="200398at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039730">
        <property type="entry name" value="Jlp2/Ccd25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13049:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13049">
        <property type="entry name" value="DUF814-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NFACT RNA-binding" evidence="3">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="138"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="138"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="182"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="206" mass="24219" checksum="893A577A62852816" modified="2022-05-25" version="1">MVLFFKISDPNYLIYMGIDKYENEDLIKFGWPEDIWFHVDNLSSAHVYLRLKKGETINDIPADVLEECCQLVKQNSIQGCKESAVDIVYTPWANLKKTAGMEAGQVFYHNENAVKVVKNVKKNREMVNRIEKTREKREVDLRGERDRRDKLEREEARAKQNEKKRADIKAAEEKKKAEELKNYTSVMKETNMKSNKFNSIDDDDFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V201</accession>
    <name>A0A8J4V201_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_000977</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000021">
        <property type="protein sequence ID" value="KAF2077730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V201"/>
    <dbReference type="OrthoDB" id="18172at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000180">
        <property type="entry name" value="serine/threonine-protein kinase STY46-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050940">
        <property type="entry name" value="Actin_reg-Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46485:SF5">
        <property type="entry name" value="CENTER DIVIDER, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46485">
        <property type="entry name" value="LIM DOMAIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="31"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="305"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="460"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="317"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="475"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="488"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2077730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="508" mass="56252" checksum="CCE72C0740AFA9A4" modified="2022-05-25" version="1">MMNIKDIQGLPFSASGDDENDLSSVEYSDLVMDSQEIGRGSFGQVQKASYFGTDVAVKSLSSLVNIDPDYFKFMLREIKILKGMRHPNIVQYIGACLNEGRYMIVTEYIKGGDLHQLLKERGVNAIPWNVKMKLAFDVASAFSYLHSKKVIFRDLKAKNILIDEQGTTLRAKVCDFGFARILDISKDMNANKEGYLTICGSETTMAPEVIVGSNYNDSCDVYSYGVLLLELILGSRVVKTQLKRSPMSAFDMNLEKAEYLAPESCPKDFFDLAKWCCAYQPKNRPSFMTIVKGLKELMASPVDQLPVKGKSKPYKDEESDYDGEQSYDSDDNVNNVNNSSIQIGLDDNNNDGSGNDDNDNINNTISGGENSGYGSVIYNDTDKSAPNIDYGGRKQQNRNIFNLSFFTPPPGVVPIKIEHEEEDEEDQVPEKSLSSLGFDDIKSLIKGSDQSSVLRAISSPNLSLNNPLDSAAPQHLSNSVTGVLSTSKPKKKTSKKSGKKKNHNKIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2P2</accession>
    <name>A0A8J4V2P2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009709</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000785">
        <property type="protein sequence ID" value="KAF2068969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2P2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="91" mass="10404" checksum="C5DCE6F72B5E7F48" modified="2022-05-25" version="1">MVWGNQLHSHLYKAKLAITILQVEYNPSGDQLAVHYRNCSLYILTRTSKSYNYISTFTLINQCRIALSINLTPVLQAMLSRSMTSSNHSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2U9</accession>
    <name>A0A8J4V2U9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009550</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000738">
        <property type="protein sequence ID" value="KAF2069134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2U9"/>
    <dbReference type="OrthoDB" id="444265at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22677">
        <property type="entry name" value="FHA_Kanadaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000053">
        <property type="entry name" value="Os06g0275900 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050923">
        <property type="entry name" value="Cell_Proc_Reg/RNA_Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23308">
        <property type="entry name" value="NUCLEAR INHIBITOR OF PROTEIN PHOSPHATASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="94"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="476"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="178"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="316"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="378"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="412"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="511"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="520" mass="59914" checksum="F113FB0577771168" modified="2022-05-25" version="1">MSDSNNQDNERKKVEVDMPPPSFLPKRKNADMPPPPSPPKQQQPQQPQQPPPTFEPIYKEPFWSKEPIDNEYQFEVIKGGVVLENLPINKKAYYLIGRLPICELPMEHPTISRQHAIIQHRSGGQLFLYDLNSTHGSFINKKRVQPNVYNPIKNGDVLKFGESTRLYVVCGGPEQIDSNNSNNNSNNSNSSKDSNGKKSFVDYYMSSEKTSDAAILAQSRDSWLKDDHDDEIRKDYENDEVQDTDDDHFGKMKKKKHRDDDDDDDDDQDENDQFYDRTSQNKDKLLKKYIQLPFNELVDKLNALDLDKIHLQTEFSKSSFSSSKQQETDSQDSLDQFMNENQHSLKKVAQQKIMSSLVDVEKEYKLVESIIKSKPEFRKARKDISSYLKSKEKEKEKEKEKDNKDKSDILGNNTNSSNSNKTTSTSTPTSTATPTTTTDKKRTLNIFKSGQEDEAQKTKKPLILLDDEIPEEYKVKKVEKKQQQTKPLTSKSLKHQQESEFVDLISRESASENSNERYGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2X0</accession>
    <name>A0A8J4V2X0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dolichyldiphosphatase</fullName>
            <ecNumber evidence="6">3.6.1.43</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_006680</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for efficient N-glycosylation. Necessary for maintaining optimal levels of dolichol-linked oligosaccharides. Hydrolyzes dolichyl pyrophosphate at a very high rate and dolichyl monophosphate at a much lower rate. Does not act on phosphatidate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a di-trans,poly-cis-dolichyl diphosphate + H2O = a di-trans,poly-cis-dolichyl phosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14385"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19506"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57497"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="EC" id="3.6.1.43"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the dolichyldiphosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.43" evidence="6"/>
    <dbReference type="EMBL" id="AJWJ01000318">
        <property type="protein sequence ID" value="KAF2072017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2X0"/>
    <dbReference type="OrthoDB" id="302705at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047874">
        <property type="term" value="F:dolichyldiphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03382">
        <property type="entry name" value="PAP2_dolichyldiphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039667">
        <property type="entry name" value="Dolichyldiphosphatase_PAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247:SF1">
        <property type="entry name" value="DOLICHYLDIPHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247">
        <property type="entry name" value="PALMITOYL-PROTEIN THIOESTERASE/DOLICHYLDIPHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="7">
        <location>
            <begin position="55"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2072017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="227" mass="26590" checksum="4190FFFEF242FF7B" modified="2022-05-25" version="1">MDEQVYTALTFVELTTVHYHHEDPFGLLNAYITLIPIGIAIGVLTLVMFRRDVRTISILLGLFMSEATNYVLKKSIKEHRPQMWKELRGKQSYGMPSSHSQFMFFFATLMTLFYLRKKINFKSQKLPLISLIGLYILAASVAYSRVHLYYHTSKQVFFGSMVGLILGFIWYNVIEKLFRPYLFPLIINHPIGKYFYLRDTSSIDNLLKFEYTNAMRAIELNSKNKHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3C2</accession>
    <name>A0A8J4V3C2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_009090</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000634">
        <property type="protein sequence ID" value="KAF2069599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3C2"/>
    <dbReference type="OrthoDB" id="10045365at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF5">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035156656" description="EGF-like domain-containing protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="934"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="516"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="642"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="538"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="670"/>
            <end position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2069599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="978" mass="106916" checksum="B4C59671DC22A9BA" modified="2022-05-25" version="1">MNNKLIVIFITLIFALFKVESQITKISPSFILAGTTPTTLLLSGSNFGLNPVVTVGGNPCNVKSGSSDILIQCQTTGPVNVPSTSVVVNSLAPFTLPVFKYNSFTQFNEKVYIYGDFMSIQSKLLSVSCNGYRLTGYFLNSTTYTFDLVKGFIESTKMTDFYDDTTTLIFSQSMAYSSVVVKSSLTFNQIILEGYNLSPLDYVNVGTTPCQVESNTGLKLVCLPPTSFFSDKITNYTMSLSLKTSTIVNNVIFQRPLFSYISALQNFTTIQVENYIGFENNLSKLSIMGLLHSPTPIVQSSSFKVYFPKETGICGYAYIADTSNPSQILRVSNTILLCPRQLMHGTNIPNNDDGVLSIYGSYLNTTIYGSTEKSMSFSIIKNGIATQTEPQSITYTDNLYFLRLPIPQGPESFVISIIPIWDDAISSSLIRFSPSLSSITRSRYNVSTLITIKGTGFITGQIDVSIGNGKCSNVSFVDSQTLTCLFDSTPIPINGKLMSVFVIFKAGPFSVTKEMFEYICTNDCINGGCSPVPDTCVCASGWSGPNCVQKTPIIVSISSTVYQVPATVTIVGNNFFNVDLRVEIVGSFTSICGDALASQDTKSITCLFKSDIKLNYIGDALHVVVYVNSTMVYKQSIFTYIRPDECPIGSNGQVCSGHGSCNQQQTTCTCEQGWETNDCSVKSLGGVGVTPPIVNENNTTSTIVTPNGNKFDIGIAMINELDSSNSNIIQSYNINNIKWKKVVKDNNTFIYSTELPNKSTLQAMLTINDKDERVYFNFGGDIIPILPKSIKYQIELYNWTFGSTQNNVEIIFKSGLTLSNDECNGNDNDNKKASTQSIDNSVRNIQITVNGETLIGTFSDRMILDGRPTYNKVNKLTTDQISKYQLDSTAAATIYTSILTSYFNNSVIVDPNFGVLISSTTQGCATGTKGLANWKIAVIIVCSIVGTAILVTTIWLVVKQNHGSVKIIALKLKRKSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3W0</accession>
    <name>A0A8J4V3W0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_005728</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PNO1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000236">
        <property type="protein sequence ID" value="KAF2072962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3W0"/>
    <dbReference type="OrthoDB" id="1932641at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22391">
        <property type="entry name" value="KH-I_PNO1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22392">
        <property type="entry name" value="KH-I_PNO1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000009">
        <property type="entry name" value="RNA-binding protein PNO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000048">
        <property type="entry name" value="RNA-binding protein PNO1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055212">
        <property type="entry name" value="KH-I_PNO1_first"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055211">
        <property type="entry name" value="KH_PNO1_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826">
        <property type="entry name" value="RIBONUCLEASE Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826:SF13">
        <property type="entry name" value="RNA-BINDING PROTEIN PNO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22891">
        <property type="entry name" value="KH_PNO1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="163"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2072962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="253" mass="28382" checksum="394F8C87075AF74A" modified="2022-05-25" version="1">MSTKPTTPSVDQEENVLSEESVQQESNKSKSNKRSRDADMTPVSEQGEENDDNVNDSNKKPSFSQITIEDTETEQIRKITIPFNRLAPLKSNWQQIYEPIVTHLKLQIRMNTKTRKVELKSSKTTKEASAIQKGADFVHAFSLGFDVNDAVALLRLDDLYIDSFDVEDVKILKGDNLARAIGRVAGQNGKTKFTIENVTKTRIVLADKRIHILGSYQNIRVAKDAICDLIIGSPPGKVYAKLKTVSSRISERF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V435</accession>
    <name>A0A8J4V435_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005525</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000222">
        <property type="protein sequence ID" value="KAF2073172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V435"/>
    <dbReference type="OrthoDB" id="9989223at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.80">
        <property type="entry name" value="E3 ubiquitin-protein ligase SopA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051082">
        <property type="entry name" value="Pentapeptide-BTB/POZ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14136:SF21">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14136">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN KCTD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141571">
        <property type="entry name" value="Pentapeptide repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="11"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="400" mass="45448" checksum="6C952CEE5337FEE4" modified="2022-05-25" version="1">MIEKDQIYSDEWVSLNVGGKIFHTTTSTLTSCKTSVLYKMFSKESNLPVSRKDVNNNWLFDRDPEYFRVVLNYLRTLPTTHNDLVIDEGVSLKGVLQEALFFQIDGLVKLIKKIESSQSDFSRKEILLHRSSIKFNNKKLNNLDLSSIEFIKESFNFSNISYSNFDGCKLSLSTFFNVKAKNATFNNTLADNVNYSTSNLKNSQFLKSDLSHSSFNNCDLSYSRYDQSNCTGSLFQRATIVNGSFKNTYLEHAKFQKACLRGCDFTGAMIQNCHFTSADLRNAIFDWKNIPPSGVVFFKKSKITKQQYDQIPLENKDRIGFKILADDAIISPKKQKYINRNTNLIPNVSNSSSNNSNNNNNNNNIVEELPAVLTNTTLNNSTNIAPNNNNNNNTNNNNNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4C3</accession>
    <name>A0A8J4V4C3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mannose-1-phosphate guanylyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008094</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000471">
        <property type="protein sequence ID" value="KAF2070589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4C3"/>
    <dbReference type="OrthoDB" id="285674at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06428">
        <property type="entry name" value="M1P_guanylylT_A_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="2">
        <location>
            <begin position="4"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="3">
        <location>
            <begin position="284"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="409" mass="45975" checksum="5930B395010D9932" modified="2022-05-25" version="1">MTSKAIILIGGPSKGTRFRPLSLDIPKLLFPVAGKPMIYHHIEACSKVPSMKEILLIGFFKEEQLKDFIQQTSKQLNVPIRYINEEKVLGTAGGLYHFRDIILSSDNPESIFVLHSDVCCSFPLEELQQFHQKYKRHCTIMGTQVAKEYANQYGCLVRDENTAELLHFAEKPETFVSSLINCGVYCFSPSFFDVMGKTMVDLRASNVNINSDYPEITRNRNLETLRLEQDIFVPLAGTGTISVFPYQGFWRQIKNAGSSVYCQELYLNHYAKTKPEILKKGDNIIGNVIIDPSAEVDPTAIIGPDVYIGPNVKVGKGVRIIHSIVLDSTIIKDHACVIYSILGWSSEVGLWTRVEGIPNYTPFLYSQEKRKGITIFGSGAQANGEIIVRNCIVMPHKQLDRSYSNEIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5W0</accession>
    <name>A0A8J4V5W0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA ligase</fullName>
            <ecNumber evidence="15">6.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">CYY_003473</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="15"/>
    <dbReference type="EMBL" id="AJWJ01000109">
        <property type="protein sequence ID" value="KAF2075212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5W0"/>
    <dbReference type="OrthoDB" id="206088at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07902">
        <property type="entry name" value="Adenylation_DNA_ligase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.30:FF:000003">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050191">
        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031916">
        <property type="entry name" value="LIG3_BRCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF9">
        <property type="entry name" value="DNA LIGASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16759">
        <property type="entry name" value="LIG3_BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="ATP-dependent DNA ligase DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00333">
        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="9 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 15" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="12 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="18">
        <location>
            <begin position="362"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="19">
        <location>
            <begin position="852"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="19">
        <location>
            <begin position="1015"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="205"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="627"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="946"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="628"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="955"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="992"/>
            <end position="1008"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAF2075212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1119" mass="127259" checksum="79F3127D367E9D1B" modified="2022-05-25" version="1">MSDTEKDKGDSSGSLYSMLTLCKDLEKESSHTGKSRIISEFTKKFDGDLYLLAKLMLVKEDKRVFRLKDKSMIKVCAHIWGADLDDMIADLDNGDFTETCKKFYIEYGNYPKRSTLTLQEVDDFLENLTKSGKFEDQVKLVKKILKKSIPSDWRLICRIIDGDLKINTGAKFFLDGLHPNAYDFFKKANNLKAVIEKIIKHDFDKNKSKQDDDDQDMDEGQDDDDNDDDDDGKKKKKKATKSTATKASGAFDSGIKLMTPVKPMLPKASKSVADVIKSCEGTFYAEIKYDGERIQIHKDGNKFSCYSRNLKPMMPHKVEHVTEYIPKATKASQMILDGEILLMDTKTGVPLPFGTLGVHKKGGFKDATVCIFLFDIMYLNGQSLIDRPFYERREILEKNIKVVQHRVEFSEITEVKGEADKGKLTDLLNRTFKEKLEGLVVKDGDKPYEPGCRHWIKVKKDYIAGMGDTADLLALGGYYGTGNFGGIITVFLMCTWDKKTKTYKTVCKASSGIDDKTITKMQKGILEKMTKISKDHSKVPDWLDCLKQYAPDFIVKDYRDAMVLEIGTAEITQTKHHTSGYSLRFPRILKVRDDKDYKTATTLEELHHLGKDAKIVPYDDDEEVVLNPSKKTTAAPTTTTTTTTTTTTTTSTSAKKSSSTTCKPTIKELPTDSNLFLVHFGNAKLWAKYGLSKNIGKYFEEDELKQFNDEEKYKSKSGTLTHMENNNFTKKKVCIFQIPCIEKKPGKDASFNIGLFKKSLGNLYSLAESTNSKVVLFKQNYPEWSDIEDTIQNHEDQIDYLVFADKTSKDSNGSNGDVAADAAGVTEKIITTTTVELLEHEIISRTEEMEEDKEWPFDGVNVVFSDNLDKDLVTRLKKYVIARRGKVSDQWVPGGINETTHFVCDAVCPMFTHVDRLGGLVVQSKWIEDCFLNDEKLNEDMYIFVNNSSNSSDDGDSKSSDSKKEEKSNSSNSSNNEDDEKKKNNISPNKRSRQEEQDNGKYQDNERDSVKIKTIHPSIFKKCNIYLHDNVNEKEKLKRYIIAFYGDISEHITDKTTHIVISLPPNFSLKPKEFIYKQLEKNFTNQKNQKIPVLNSLWIWDSLNNEKLLDESKYILTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6E3</accession>
    <name>A0A8J4V6E3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Signal peptidase complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_002934</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Enhances the enzymatic activity of SPC and facilitates the interactions between different components of the translocation site.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Endoplasmic reticulum membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SPCS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000087">
        <property type="protein sequence ID" value="KAF2075752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6E3"/>
    <dbReference type="OrthoDB" id="29558at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009582">
        <property type="entry name" value="Spc2/SPCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13085">
        <property type="entry name" value="MICROSOMAL SIGNAL PEPTIDASE 25 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13085:SF0">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06703">
        <property type="entry name" value="SPC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2075752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="183" mass="21341" checksum="4EBD6944F0624F34" modified="2022-05-25" version="1">MSDENEKKEKPVQVTLYDSLSMKQTLDDAVVKYITDKLGYKQNHKMNYIKVLLGFIGCCLAALAQFYPVPFPQNKPILIVCVFFYAIISTILYYINMFIQKDYIVFAVKESNEKEIQVSSTLPRYDPNYTIKIEYRNISTPTPITFTKSIDQYFDIKGYFVQDKFFNDLSGVFKKFITTNKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6F7</accession>
    <name>A0A8J4V6F7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Leucine-rich repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_005895</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000247">
        <property type="protein sequence ID" value="KAF2072794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6F7"/>
    <dbReference type="OrthoDB" id="20811at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027038">
        <property type="entry name" value="RanGap"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113:SF12">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="270"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="383"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="278"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="383"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="429"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2072794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="670" mass="74645" checksum="14B621BF7852C08C" modified="2022-05-25" version="1">MNINQLPITLLEHILQLALNNGYSIGLHHLGDNNDNSSSSSSLISSPNNLSESSSPIVSPILHSHVIDKSNSNYSPDLFNINSSSSSISINSNNNSNSGNDFCPLVNYYNISIVCKLWKDVSKKSFFSLHLSPLALYKVNQSNKIYNSIISGSSNNSRRSSIAAIQVCSSLDDNNNNSSNNSNNDNDLNNNNKRVFCENNGSNDSSPCNNSVEINLKPFKNLNIHQFFEVFQKFTSVKTVRLSNFNFIKTNSSQPLNLFKRLKRGPSPTLVGEKVCESNSNNNNNSNNNKNNSNNNNNNNTLNKNNNSNNNNSCFSTTSILLGGMNSPFMMSSFHFPPSRFDIPNFNLNNQLYIPTTTPLISKPPNNNSNIFINNSNSNNLNHIVLNSNNNNNNNNNNNNNNNNNNNNQNNSNLNTNTNNNNNDTDNSKLNISHTTNTSPILKDNNLTKKIKLFSSTSALDEYKVNQIFYENTISNVKKLEIVSCSNFKSIEFINYFLNSSFLKKLVINNCDLKNWDCSVIFFTVRSSESLTSVDLSNNKINEEGAKTISIVLKENKTLKEIKLNNNQIGSQGMILLSQSLHENSTLQSLSVTHNRIGLDGGLSILQTLHSNRSLTSIDLSNNLFSSPELLKSYCMIFIQNQTLVLSPFVKVKVNSTILNDNVDQLDEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V747</accession>
    <name>A0A8J4V747_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bms1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002227</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class translation factor GTPase superfamily. Bms1-like GTPase family. TSR1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000059">
        <property type="protein sequence ID" value="KAF2076487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V747"/>
    <dbReference type="OrthoDB" id="119302at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012948">
        <property type="entry name" value="AARP2CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039761">
        <property type="entry name" value="Bms1/Tsr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007034">
        <property type="entry name" value="BMS1_TSR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030387">
        <property type="entry name" value="G_Bms1/Tsr1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858:SF1">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN TSR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08142">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04950">
        <property type="entry name" value="RIBIOP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22298">
        <property type="entry name" value="Tsr1_G-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00785">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01362">
        <property type="entry name" value="DUF663"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51714">
        <property type="entry name" value="G_BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Bms1-type G" evidence="6">
        <location>
            <begin position="87"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="377"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="419"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="469"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="37"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="395"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="429"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="475"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="490"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2076487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="809" mass="93774" checksum="23DD6DE632F03497" modified="2022-05-25" version="1">MEHHQHRSGSLKQSNKGFKGGKHDSKGAVKRRAQGKVETNQPRQSLKNLAIAPSKFEIQKQKQKHLAQSKKNEILERRRLGLPDLLPPRIVSIVSLDENCDTSKIKTLLLQKLVNNGTVDESSMETTTGQENTVFSTVSITSKVRMMLMECRSNDVEIIVEYCKLSDIVLFVVDANQPVLSERAERIFSIVKAQSVPTVMQLIQNFSEIPLKKKNDLKKQFVSSFHFHFPDEPKVLPIDTEEDASQVNRYLENIHINQIVWRNQRAYMIIEKAQVVPQSDIVTIEGFVRAQNLSAKQIIHIPDFGDYQIEKIELVADPHIRRKSFYTGEMDDTQAPTIIDQAEKQDRDTIQTYNTPDPLDLEQPIITNEEIEMAKNKKKKLVPKGTSSYQSSWYLDDDEQEDQDDVDDQEMDNMMDDEQVEHEQQQMGQDEEEEEEEEEGVEEEKEEVEEAKTEDNKWKYIRDKSQITDELLKESDDEGNDMQSESEDEETRRKDERTEIEFPDEVDCPHNVPARIRFSRYRGLKSFRSSPWNPKENLPLDYAKIYQFHSFNQSMKNSIALLERAPVKPGVYVRIHLVKGPKQLVDAINVKPHVAVGLYRYENKISVLHFNVEKYKTYQDTVKSKETVFFQYGWRKFSTNPIYSLSSPNCDKQKFEKYLFPGRNTVATIYGPISYPPTPLLIFKNSSCDDLIATGYLSSINPDRIICKRITLTGFIAKSISKKFVTVTDMFYYPEDIMWFKKVELYTKLGRTGHIKDSLGTHGRMKCLFDGTMNQQDTICMNLYKRVYPKWIDENSNLLVSEEQAKLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7L3</accession>
    <name>A0A8J4V7L3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Alkaline dihydroceramidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_001728</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alkaline ceramidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000043">
        <property type="protein sequence ID" value="KAF2076952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7L3"/>
    <dbReference type="OrthoDB" id="15687at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006672">
        <property type="term" value="P:ceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008901">
        <property type="entry name" value="ACER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46187">
        <property type="entry name" value="ALKALINE CERAMIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46187:SF2">
        <property type="entry name" value="ALKALINE CERAMIDASE DCD3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05875">
        <property type="entry name" value="Ceramidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="208"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR608901-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR608901-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2076952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="291" mass="33624" checksum="076D5F09836E205E" modified="2022-05-25" version="1">MAEIQESIEQQYYWGVPDSPIDWCENNYEISPFICEFYNTFSSFIISFFALYGIYLLTSISMSRDEYTTQHIKIVKQVGIRAKVLLSYSSLAVVGVGSAFYHATLLYKNQLFDEFPMIVTASLFIYCLLTMEPVSKSDSKYYQIFRWLLPYALVTYFLIVSTTIFIIRNVPTILQVSFGVLIVSNVIISTLHVRGLNQDYSVSNQKKLLTYCTVSMLIAYFSWLAERKLCNEYGYVIPGLQLHAVWHALTGLAGFYWVQFYICSILEKNQYKTNIIWNWGVASINGYFKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7N2</accession>
    <name>A0A8J4V7N2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001706</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the gonadal family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000042">
        <property type="protein sequence ID" value="KAF2076997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7N2"/>
    <dbReference type="OrthoDB" id="21617at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010849">
        <property type="entry name" value="Gonadal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13054">
        <property type="entry name" value="DIGEORGE SYNDROME CRITICAL REGION 6 DGCR6 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13054:SF2">
        <property type="entry name" value="PROTEIN DGCR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07324">
        <property type="entry name" value="DGCR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="304"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="8"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="249"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="260"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="488" mass="56801" checksum="1B06A2F521C07D11" modified="2022-05-25" version="1">MSDTYSKNINNYNSNNDYNNNNYNNNNNNNYNNNDTSDYEDHQYQTNQSDSEYNPYQQAEILEEVYDISDDDVDQIAKLGLITRTGEDDKENIDWLIDLAQIHINQYPSPNQDLAEQEKKILESFETINKEYSHEHTKGKYSKLVKELYKSANRKFRNPYPLLKLYKSFREIRKEYEAYTLNQYYIKKYRKLPHLFNLEYNKQVGAKVNEQSAVEKAERKKKRKKLEDQEKIVTMPVGTPIPLILQKKGSTNASTEPQPTTTSTTSATAVASTDFSLDSKLLDALIPIDSLSQKNIAGFASKIIDDNSNNQNQPQQSQQNTTSGTPQKPQDQQILSDLIKLSQDPLVLDQVKQLLDQQRFKEAQLLSQREELLHNQLERKKFHLQKFCDQLLECGNDNNLKKEIQRKLSLEAKMVHDHSQEELRQFSLKIVNEMNNILTFQQLSLQRLGIVGFFPTNNSVHLEQQMKILECLFPTLIETLKRPSPFRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7T4</accession>
    <name>A0A8J4V7T4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001579</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000038">
        <property type="protein sequence ID" value="KAF2077132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7T4"/>
    <dbReference type="OrthoDB" id="9991235at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008832">
        <property type="term" value="F:dGTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006203">
        <property type="term" value="P:dGTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3210.10:FF:000030">
        <property type="entry name" value="Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2760:FF:000004">
        <property type="entry name" value="HD domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050135">
        <property type="entry name" value="dGTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045509">
        <property type="entry name" value="HD_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373:SF4">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE SAMHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19276">
        <property type="entry name" value="HD_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="2">
        <location>
            <begin position="625"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="125"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1002" mass="114881" checksum="02708358049C70F4" modified="2022-05-25" version="1">MSTTTIDQYTLFKDKLPEYSQEGFDNDYFEEKILEPLSSNVNFLERSSNVLSPTKHQSPIKISSPKPKSSNNDSSDEDDDNNSNAVGAYFCQLCKKRFQNENTWNTHIGSAKHIKLVKESKKKSSSATTTVSTPKGNNNTPTKDKKKSTDTSTPTKTTATTTTTGSNNKQQKESSFTFLATQKEELQKVLKIKSSKPNLACKSLFVIAKAYSQNHYVRDSAKCLHYILDVLSATATTTPSPTPTPTSTFHINTGNIYINATLSSKTNLFLARLCRLFDQQLSDINYIESFYKLNIITKEKLQSLATSLSNNTLDPLLLLNDWIGQITLHQQQVAAEVQDESSNNIDEEKEKKQQQIIAMYNMLNEVAGGLCFSKMNYQSIVLYLLSSSIAYAHQLMSQYHLAIKRIIKLLESSKQYHIVIDLIKNQLILNNNNNNKNNSNSDSNDSTTTLIPTPTIMTIIKYCLVIQDRIRLKDALLYLNNNRNCYDEQDETIQCITSIGQSYIDNDYNKLDDIKDEYVDDDEMEFDGPGFAPESTPPKFTIPHNFSSNLYQTPSTPSSQEYAYNLIKTSKIRKQSKIINDVVHGHMDVPDYILEFVDSEQFQRLRDLKQLGTTSFVFPCGTHTRFEHSLGVSHLAGKWIDKIKMTQPDLEITEDEQKFVRIAGLCHDLGHGPFSHAFESWANTLPTKRFHHEEMSVKMLNWLIDDKGLDYSTDDVKYISNLISGKNRPKERGFIYDIVANDRNSVDVDKFDYLARDSYYLGRSTVCDFTRLIEFSKVIDNEICFCSKEIYNLYELFHTRYSLHKLVYTHKVAKSIEFMISDAFSAADPYLNISDQLEDPHEFINLSDSLLRTIETSKVKELEKSRNIIKNIRNRNLYKFVDEIIVSSKNMPLELAQEIAKEGNGITVDDVIVQNLKLNYAFKDKDPVEHTHFYTRYDDSHKIKIKKEHASHLIPNTFQEERIRVFCRSKDKCEMVQKSFRKVLKNYSITPNPSFTVSPYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V856</accession>
    <name>A0A8J4V856_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chorein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004019</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000135">
        <property type="protein sequence ID" value="KAF2074669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V856"/>
    <dbReference type="OrthoDB" id="43807at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026728">
        <property type="entry name" value="BLTP3A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22774">
        <property type="entry name" value="CHOREIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22774:SF11">
        <property type="entry name" value="CHOREIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24917">
        <property type="entry name" value="BLTP3A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="263"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="551"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="860"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1144"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="289"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="301"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="335"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="561"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="590"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="605"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="873"/>
            <end position="887"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1215" mass="136513" checksum="460E79250D1E3A66" modified="2022-05-25" version="1">MEALVLKVLKKYLKLFIKNFTSDNFSMSVLKGEGTLVDLDLNETIIQELLLIPPQFKVTHASCDLLTAKVPWTSFKKEPIVISLNSINIDLKEPDEIIPVVSQLKKFKKKNKNKRNEITENLQIEVKSLKLNVASLYGNTLMIEIEDVLIQSTNSNFQPGDLLQIKSIDKEQGIESLHKLITARSISIRIQDMGGMTTPILENMPIKILYCTKRRIKDWIQLSAKIEFVLKQINLRWTLSQWHSLNDLIHGFQATLARAVPQATTPGVGAPTPKEHKNKLFSSKKKDSISSGTSTPVNIISSTNSLGNSSTTGNSPLSTSPSISKLKESGLGGDSSTTSSLATSPMTSSTGEFGVQPTPKHSDFSYDFHIERWKLELVDNYSQDSDSGYHFKGEGLHFGFTSANTVAKPINPDTNSPFYVMPIRETILSVVMNTLSVNQVLSFSKNISKATLADTNLESRGTKVYNKTITSISSESIPERISIHNYTGPFLLKGNLLFRKPITNQDNPDPLANIVHGPPLIGLELNLHLNDFKLHGDRRSWKTLISFLAPPESDESDIGSESESHSTTTSTTKSIIDNLESVSLDDKNDQQNGKQENSNNGTQQHQHHHHKGVIEKGKKKISTFKRKLKLGDHWKNQIKIILKATNTQLTIPEEKIPEFQGLKLIINLGSFVMCNHSDWKVVPYLADGLQFIDNKVPQADSITVSGRDHRFSFQMENIALSIIDTKDSSVHPVLEPSTISLFLRISRTNTLLMEKRIPKIDVSFISSDFNFKITTQQSLFLDHVANKYLSPKKMKNLLGARISKEAKKRLKSLDDKHLDKTITIKNKVEKTLQRYYWNSFISIQKGDFFLPLSHFLEPKEKSSKKGDNDDNNDTTATTTPQGATTIDTPLEMISNVVQDLNLIPQSIITSVDNDEANQSSAENQPLSQLKVENLGIILQNNQQGQTIIFKIGSFEAYGIDHPKLSTSTILRPLPIPDDEYIPAPNTDSTNLFVTYKRRQKALAASTRSNILIDEEMEEWTTDVWVKLQGTQVRVMKKHLSPAQKMATKSLSKKIKMPDVKNFITKVVSLVERKRDKISQVKKGIKKVNLDIKWGIELGNCEILLGEKSKNSIYSCSDFEIAPDYQPKGIIKITDTNRKINAKAYRDIEEELLKKNSEFATAEEEKDEYNNRIGALVAEIDKLKKTHHTELSALRSQYEELEVKFVATKLKLAEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V886</accession>
    <name>A0A8J4V886_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000025">
        <property type="protein sequence ID" value="KAF2077567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V886"/>
    <dbReference type="OrthoDB" id="10526492at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035290265" evidence="1">
        <location>
            <begin position="20"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="144" mass="16676" checksum="75A527C91D2F9088" modified="2022-05-25" version="1">MLKILILLILILSINLSVSIKFFIKNGESWEQLAATNSVDDSNLVYQINLKKEENFEFMVVCNASFEDLPPRTYTVDITSDGTEEAILYRDYIHNRYEGKLPYHVDYCGYNLFPFLLTNQNALLNGDYIFNISRLGLCNFIDIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V9V7</accession>
    <name>A0A8J4V9V7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phosphoethanolamine N-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.103</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_002192</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N,N-dimethylethanolamine phosphate + S-adenosyl-L-methionine = phosphocholine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25325"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58641"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
            <dbReference type="EC" id="2.1.1.103"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:25326"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N-methylethanolamine phosphate + S-adenosyl-L-methionine = N,N-dimethylethanolamine phosphate + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25321"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57781"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58641"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.1.1.103"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:25322"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Phospholipid metabolism; phosphatidylcholine biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.103" evidence="5"/>
    <dbReference type="EMBL" id="AJWJ01000058">
        <property type="protein sequence ID" value="KAF2076514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V9V7"/>
    <dbReference type="OrthoDB" id="18809at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000234">
        <property type="term" value="F:phosphoethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44307">
        <property type="entry name" value="PHOSPHOETHANOLAMINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44307:SF2">
        <property type="entry name" value="PHOSPHOETHANOLAMINE METHYLTRANSFERASE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01209">
        <property type="entry name" value="Ubie_methyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2076514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="284" mass="31722" checksum="D4C998E05B61B819" modified="2022-05-25" version="1">MSTNTMSTNTKNQIDQTPEGWDTISKAYSHGFTQLYAIDALDLTIGKNGTKDDIKILDVGCGTGALTLNALYKIKDSKNSFILSTDFSPSMLKILSDKVRENKIDNIETKIMDGQNMVGIQDNTFDFAYSVFGLIYFPDRNKGFKEMHRVLKASGKVAITSWLSTTPFCEMLTTGLSHFTTIDAKQSPYKKGLLSLADKQQFKQEMIAAGFKDVEIHTVHHNMVIPAGTSCASLFHDNPVYECLFDYIPREKQDELDKVMDDYVSKNYPNLILNSAGYIGIGTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJ79</accession>
    <name>A0A8J4PJ79_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010413</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001097">
        <property type="protein sequence ID" value="KAF2068260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJ79"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF29">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="90" mass="10112" checksum="42FB7CEDF90C50FD" modified="2022-05-25" version="1" fragment="single">YIGIGIRSYHRLVQLDPDFSVLVDQRPAADNDNSVCSSSSKSKLSKAQLAGIIIGSVAFATILVTVVVYYVYKRKQKEFFESKLKLKRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJ93</accession>
    <name>A0A8J4PJ93_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010357</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001059">
        <property type="protein sequence ID" value="KAF2068320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJ93"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="37" mass="4245" checksum="78BAF36EE9FB914F" modified="2022-05-25" version="1" fragment="single">NNNNNNNNNNNNNNNNNNNNNNNNNNNNNSAKKNFIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJE7</accession>
    <name>A0A8J4PJE7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010102</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000923">
        <property type="protein sequence ID" value="KAF2068575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJE7"/>
    <dbReference type="OrthoDB" id="26679at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="114"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="61"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="279" mass="32220" checksum="B2222DF393995FAD" modified="2022-05-25" version="1">MSYHETYRDGTHFNYILNYLRDEGDIQIPEDIRHCVRKEMEFYRINDHFKLDDTTTIIDHLNSLKLKLDETEQELVLAKEESNKTRKESEKLKKEFNSFRNKEVKFLIQSELSSILNSSTFEIINDWIDEAKLTNFELLYYASESNKFSAQRFHSACDGKGATITVIETTDGCIFGGYNSQSWNDTGKFYGDNRCFIFTLVNKHGIPPTKYLPIKTIRSYVGSSIGQGLVFGYNDFKILEEDSFQSFPTSYADTTGKGNTTLTPSQVFSIKNLEIYKCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJI2</accession>
    <name>A0A8J4PJI2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001168">
        <property type="protein sequence ID" value="KAF2068154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJI2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="419"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="450"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="536"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="689"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="187"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="219"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="306"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="340"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="423"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="483"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="571"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="582"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="620"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="708"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="722"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="762"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="785"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="785" mass="85591" checksum="F29FEDAB6864A90F" modified="2022-05-25" version="1" fragment="single">MKKTSSFVDNENNNINNNNSNNNNSNNNSSSNESIPHHRTTFKDRLTATSAKEHNHHLLSPRRIHSTSNIHSFAPGWSTNKSIELIHIDLKKIKQTSNTKETTVQEQDDSVQLGSSSEFDGSKGNSILLQAKSQLDIFDSPRVSTPTTTTTNSTTTTTTNTSSSSSILNSPNHHRAINNHSPLTIKPSLSLSPSPSTQLQQHNHTHHSLFQQLPTNVSTSSSISSISSTSSSSTISTSSSISIKKTNEEEEDEDEYSEDDIDYKDKKNSMAAFLSRIQHQQQQHQQQPPPSIIKKVMPLKPLPLDVQKSQTTQQQQDVKKYKSMKHPLTRVYSAPTIRFNNNTNTNTNSNVSSIPTPSSPSHQHQPPLPISPQQQQQQQQIVSSPSMSKLKVPLISLKGLQELNGLEVAVKTSPRLQQIELQSSPSSTTPMSTSPSDLKILKNRPILFNLNTESEDSNSSSSDSDDYDDHRVSAKSHAIQPTNINNNVIHTNINNSNSSSNSSNNINNNINNNIPTRPMLHSRKSLSNIFHIDISPHPSPLTSPIASPRDYLKPSSSSSDDNNLHNHPKKERPSSIRKRSDQTSPSLTPRTTLKNRSSSMSPISHDNNTSPSSSFLNMGPSSPSSNSVNNSNNNSSLTSSPSSLPLTTNSSSSLTTTTTSTTTTTVIGGSSNKPKFALNLDQLGSNTHLSNIPTGASSARLPPRKLSGTGNTYFSNKTKINTPLSTSSSPASILISPRKHHTLDSSTSSSPSSPPCSHDDNNNNNNNNNNNNNNNNNNNNNNNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJI9</accession>
    <name>A0A8J4PJI9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009936</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000859">
        <property type="protein sequence ID" value="KAF2068740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJI9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="195" mass="23145" checksum="95142F57A5069844" modified="2022-05-25" version="1">MDTLFFSVWRNTVFRRYIRILVCENLVINVDLQYLNDNHQYMSLLSDQERLDNNIYFKLLISTRDELNEYNDNPHKHLIDDIEHSHFNLRLNYNVVPAEENYIDCDKICDGVRRLLIYADENTRVRGKLPQSITEFNLNGDDDELYYMASFRNREIDAMVSGLPNLRKLVLPDNFSFANKVELPSFCKSITFSMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJJ2</accession>
    <name>A0A8J4PJJ2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010084</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000916">
        <property type="protein sequence ID" value="KAF2068592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJJ2"/>
    <dbReference type="OrthoDB" id="10059102at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050966">
        <property type="entry name" value="Glutamyl_endopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15462">
        <property type="entry name" value="SERINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15462:SF8">
        <property type="entry name" value="SERINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase S1" evidence="2">
        <location>
            <begin position="58"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="754" mass="85887" checksum="164390502D7E03F1" modified="2022-05-25" version="1">MDSISFRSDLKFDVLNNYDGYSRKSVSGLCQFTYPNPVITANWPISTIGQIKTKINGLNKETFFGTGALVGPNQVLTCGHNLVNEDKDTRAGTFGWSSEVYFSIKDSQGRLQEIKATHLLVHPEYRKTRSPKYDVGLLILEKEIGYELGWMGIFSADPDYSQGYSRSTQVFTNGFSSKSEEKQFELSKDIKSIKLTNSFEYTKVPTEKGQSGSPIWKRWPSIFSKQLVKNSDLCVFVFGIHTQEGKGVEITSELFNLIVSTIKSSHIYKDVGLELNPYIQIRDLQFDTSSQKPFLNQAELETDPLVKESFYRKSFLCTNDFDTVAAQKYANLLVELYSSTKDSSFICEAYNLYFKLKSYPHLFDTAEANTKLIESAYPFWTGDTKNLNFLPDISTKIDKKVIEQPPREINKSLVYIVTSSFEQAQALNSIFQKFGPTYQVSESVESKFQFSIQISNRPINIIVTFQNSISDLKDIFNETPALVLNVGLCAGNLSSDKNVYLGDIIIAKKAFFYDGGKRTNTGDLRNNNSEHSSFADQARSLMEDKTNWKAYPVIEKQPDHLKHLIYRVLYSIGENNSDKYLEDYDPKKMISSYKVLSAYKPTEIVRELDTMKRKGEVKFDQKFILSDTLKETVSLSLQQYGQYPLSYSNESPKALFGTFGCGTALEEKVEFVDNVKKSAAFIKCQEFDYETIAIDKETADFYKVGNDYNTSKFLSIKSVSDYADDESNSYYHPFCYQVTASYSLELIQNYFLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJK4</accession>
    <name>A0A8J4PJK4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RZ-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_010445</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001118">
        <property type="protein sequence ID" value="KAF2068229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJK4"/>
    <dbReference type="OrthoDB" id="2423195at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046439">
        <property type="entry name" value="ZF_RZ_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20173">
        <property type="entry name" value="ZnF_RZ-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51981">
        <property type="entry name" value="ZF_RZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RZ-type" evidence="7">
        <location>
            <begin position="29"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2068229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="116" mass="12834" checksum="C69DDD4304C3CF9E" modified="2022-05-25" version="1" fragment="single">PINSSQLNPRYKDTINDTWADIEVIKAKLRKRVLREIASVVQAMGGAAGHWFKCSKGHHFYIGECGGAMQRGICIECKEVVGGSHHQLVSTSSHSDIDGSVSQLYKPMEIDHNQLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJQ6</accession>
    <name>A0A8J4PJQ6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009748</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000794">
        <property type="protein sequence ID" value="KAF2068935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJQ6"/>
    <dbReference type="OrthoDB" id="10250320at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF35">
        <property type="entry name" value="CYCLIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="27216" checksum="BC8376962162CB14" modified="2022-05-25" version="1">MSIYQQIFRKFYSEKSGKHKDSWEYFVLGLRVYQWLSDNDPAYTNNLSLDEILEAIDEIFDIILSILEMMKSPGDLLTPIVYYANAFVNRAGIKHNQLFNLLLTSTIVTLKFWSESAPTNNKMMADIFEFPVNDINLMERRFLCGIDYNLQLNQTQIDNFLSKINITSALKDSCKQFSRSKMSSSNVNIVSSSPSSSSSSSSRSNSIQKISSSSVNSNSPINANSFSTTAAAYTHPTPIANC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJX0</accession>
    <name>A0A8J4PJX0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010354</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001059">
        <property type="protein sequence ID" value="KAF2068317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJX0"/>
    <dbReference type="OrthoDB" id="22274at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="144" mass="16347" checksum="F3DC12BECD49D441" modified="2022-05-25" version="1">MCVHDDIYIPAPNTFNYQLFEDLTGDLDNFPLIGNGCTEKCKKKMIKIIKILMKSFTQLAIGYFQDAKLGLDRSAPRCDLVFYISYIQENGVDDDEDFIEIVESAMNVKSVNGEVVHLKQDDTQSQDLSNLTKNGKKKVSNKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PK61</accession>
    <name>A0A8J4PK61_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009353</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000687">
        <property type="protein sequence ID" value="KAF2069325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PK61"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1105" mass="129185" checksum="6A7B4E4B455A94AA" modified="2022-05-25" version="1">MFIFLSLKKIGHFLDQDIQFFSHISTQKLSIINQLISFLPPFLLKFSQLQNMFMNPHRPDCDFKLFVNGVSHLNNKLNGLGQGAIDILIKSFIFIGPEIINPLISLAKEDTTDSDLTKIIEILFHMKYNSELSDDQIQTYINYTNHLLDRNIDGYEDKLSTVLVVLSGEHLKQFNNDEIQNIINQSKQLINQSMSDYEISSIISTITKYQYHNQIQNIINFTLQLFNQKMNGKDISKIITTISNLITKYQFSNEQIQSIINHTKQLSNGNNHIINLEELLSIISTLSNLITKYQLNNDQIQNIVNYTKQLISPVAQFFKNGYTIAVSLVILKLSKGITKFKFNFDQIQYIINYSKEFIDKRENISLHFNIIFLIIKYPLNFDQIQNILNYTKQLTDPPSGLIISTLSEQITKYQFNNDQIQTVINYTKQLIHFETMTENHISSLISTLSKQISDNQLNNDQIQVIINNTKKLRHSCLQLECSTLPSLLSKQMLEYQFNNDKNGIIIKYTTQFINENMKDSYEFLLIKSTLLKQMTEYKFDNDQIQNFINLTKQLINQNMDIYEISSIISTLSEQQTLYQLKNDQIQNIIDYTNQLNNQIMIGNQKPKIIQALSYQIGNHGYNQCQIKNIIDNTKQLINEKISGVDISSIISTISDLITKNQLNNDQIQNIINHTNQLINGNNQNMEELLSIISNLSKLIIELNFNNDQFQISINHTKQLINEGMSGYEISSNTLNLSREMHEYRFHNDQIQNIINYTKQLIHQNMDVQEICGTFRTLSYQIFRFQLNNDQIQNIINVAKQVINQNTNDKLIIRMKLSQIISLITELSIQIFQDQFHFKESSKNDIVLNFCFHLDFLSSFVYISSLYNNNPLAFEQSYISFLSFYLTRDSVTFLNLLEMMITCNSELRNYVVAKMTEENWKWGDFDTQTLCLKNYPELQSIKDFPPTINDRITIPDLKKEDLNVQSCDFDILCRQFSFHPKLFKKSVFVSFKKIQNYLNDQHDFQFLIFQSLFPYDDDQLELVCGIIGKLHHLNQEYFCCNHHISNNIQRLYYYDFKFKNIGGVNNNFLQLLDSHLSDIDNNSKPEWMKTVLLNTFNPKIKFNGNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLC7</accession>
    <name>A0A8J4PLC7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Short-chain dehydrogenase/reductase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008661</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000556">
        <property type="protein sequence ID" value="KAF2070017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLC7"/>
    <dbReference type="OrthoDB" id="5545019at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42901">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42901:SF1">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="264"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="322" mass="36451" checksum="612BAA268CFC2E69" modified="2022-05-25" version="1">MISIFINSFVYYIPIVCFLLILFNKIALYIIVLGSKEQDIRKKYQCEWALVTGSCAGIGKEICDRLARQGVNIVMVSKSKDALQEYSKQLMSKYRIRTLTLSIDLKKSDSVQQLFDLTESIDIGVIFNNAGYLTMEGFDQTTSKDKIEMLECNSIAPMRIAEHYYRKWLDQGRRRGAIIFTSSSTAVCPTPFSVMYASSKSFLSTFAESLSIEARHQSIDILAIRPGLVKTHLFDNVPKHMILKLLSLVSQSPKDIVDVMFKSVGRYGIVVLDVGIFGILSGILRNIIGVNMMASLVSFTSFYLLKDFSIYYKHINNKKKQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLS3</accession>
    <name>A0A8J4PLS3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ABC transporter D family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_009271</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000667">
        <property type="protein sequence ID" value="KAF2069414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLS3"/>
    <dbReference type="OrthoDB" id="422637at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:long-chain fatty acid import into peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:peroxisome organization"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:very long-chain fatty acid catabolic process"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LYSOSOMAL COBALAMIN TRANSPORTER ABCD4"/>
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        <property type="entry name" value="ABC_membrane_2"/>
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        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="157"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="119"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="515"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="477"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="482"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="494"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF2069414.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="733" mass="84160" checksum="C28AC5A0CCEE2961" modified="2022-05-25" version="1">MSIKPDMSKNYNINDIDDSEGSDEMDYDFDHTISYVDEKSPILGSQQQQQRNRKEKTSIPINDPSSSSGSSDEDTRKKQQYYQQKIALEQMKKNQFDWALFKRFCGIIKILFISPLIPVILVAFLFGVATSQTYVSKFTGILLSNVYMSFTTGDKSLFVSSLIKGFIFIGISAFMDALIKFVVSVMAWRWRKTLCLTLQDLYFTKNLYYKIIAFDDRIDNPDQRLTSDIDNFTTTLASVLSQCITGPMVVIYYTYLTWTTISWYAPLIVYGYFFLGYFANKLVMSPMVSINFLQDKLEGDFRYLHQRIRNFAESIALHNLSKDKQDGIELKDSPSSSNRPKKAKLHNYRYISDSQDSNLVEEEQAKKQFENLLNNKKRVIFWQFGLNTTSDLFTYLSPLINYMIIALPVFFLHTKAALLPSDVTVQSFNCIMLASGFSQYINVSTSISDLSGYISRIATMIDVCQMVANEQSFETKVEKESNINSNNNNHSKSTDIGTPSEKSNSSIVLNQGDFINLDDISYFTPRKHPLLSNITLTIKKGENLLIMGPSGSGKSSLIRVINGLWPFFHGSISKPQNDYLFFLPQQPYLIFGSLEEQVLYPYSKKTHRISRPDLRLLFSRLDLGYLLDREKAIRKGNSINDLTHNWLNSLSPGEQQLISIIRLFYHKPKFALMDESTSSIPQPLEERVYAIAKELGITVISVGHRVSLLNHHDKLLRFDKEKNWYLEQIKKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLT6</accession>
    <name>A0A8J4PLT6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008349</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000506">
        <property type="protein sequence ID" value="KAF2070332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLT6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="24"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="171"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="249"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="638"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="318"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="112"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="178"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="204"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="286"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="638"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="672"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="704"/>
            <end position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="735" mass="84268" checksum="D7865574B33AFEEF" modified="2022-05-25" version="1">MAHFSGGAPSKSYQHHHYDYSHDENINIPVTPIPMKKPAQQYHHQSGGGNVNNTPTKTSSNYRPTTTSTTTPGRQQQQQQRQQQYDDDSAYDVDIPTIKVNGHQQTKTPFKGSGHSNGNSNFNGGASTFNPLKHGSQSFVNGINGVDETIEFQDHFQDDANHIINNINNSQYKRGNESEQPQYQPKQQSYQHRVHPQQSKRENGQQQQPQDTYENPSRQQTHNIQQQPTTGNFSQDYVVNLQLQLQSTKEELSEERTKVRKLKSDIEQQQKQRQHQQQEDTDDYHYQQSHNNQQYQQSPTQQQQQYYQQQQKPNHNQYEYLKKQIDSYKSQIDDLNEELHQEKLKQKNNDELSQEIYNLNNSNQELHDRYLKELSKVSRIDTMKRQLEEDNNNLLETIERLKLNNEQLVQDKKRAEEKTSEREYDLSAAAKRIDELEHTVQEKEYEYQQLYEEMEANSKLKEDIKELQQYRDSLNKDYENAKKRIHDLESESARHKLVLKEHSDLKTQCLALESQNIELAKTLKTNTTNAKMAIEAQKQSLFNAETKIKELEKLVQHSNGHSEYLKAELNKVNNQYQTSLIENKSHIQQLTQHYDVISQSKELQINHAKDNLYHVLHIIERNNPDLKKFIPAQILTSISSNSSSGSGSSTSSSSSSVGASGSTSGGSGSVGGSRRPTPTPTTTPISPSSATTTPSPGRPVPIYRPQSQPQPSQSQSQSQPRGNNSSSSSIGSFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMS4</accession>
    <name>A0A8J4PMS4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_009475</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL43 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000719">
        <property type="protein sequence ID" value="KAF2069201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMS4"/>
    <dbReference type="OrthoDB" id="10258345at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.30:FF:000002">
        <property type="entry name" value="60S ribosomal protein L37a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00327">
        <property type="entry name" value="Ribosomal_eL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050522">
        <property type="entry name" value="Ribosomal_protein_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00280">
        <property type="entry name" value="eL43_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003058">
        <property type="entry name" value="PRK03976.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2069201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="91" mass="9971" checksum="0741833A8A6AE5CF" modified="2022-05-25" version="1">MAKRTKKVGIVGKYGTRYGASLRKQVKKMEITQHGVYTCSFCGKDAVRRTCVGIWKCNGCKKVLAGGAWTMSTAAGATVRSTIRRLRELNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMS8</accession>
    <name>A0A8J4PMS8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphatidylinositol glycan</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_008892</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000595">
        <property type="protein sequence ID" value="KAF2069785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMS8"/>
    <dbReference type="OrthoDB" id="192611at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003923">
        <property type="term" value="F:GPI-anchor transamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1460:FF:000021">
        <property type="entry name" value="GPI-anchor transamidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028361">
        <property type="entry name" value="GPI_transamidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001096">
        <property type="entry name" value="Peptidase_C13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48067">
        <property type="entry name" value="GPI-ANCHOR TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48067:SF1">
        <property type="entry name" value="GPI-ANCHOR TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01650">
        <property type="entry name" value="Peptidase_C13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500138">
        <property type="entry name" value="GPI8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019663">
        <property type="entry name" value="Legumain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00776">
        <property type="entry name" value="HEMOGLOBNASE"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035293045" description="Phosphatidylinositol glycan" evidence="7">
        <location>
            <begin position="26"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="373"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR019663-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2069785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="412" mass="47929" checksum="836DE8EFF3C3B4B8" modified="2022-05-25" version="1">MKINIYLFLLYLLTIIAISNNVVNGQSNNIKHETYNPKTVEQFYRGGHTNNWAVLVCTSRFWFNYRHIANVLGFYRTVKRLGIPDSQIILMLADDMACNPRNSYAGSIFNNENHRLNLYGDNIEVDYRGYEVNVESFIRVLTGRHEPEVSRSKRLLTDEKSNILIFLTGHGGDEFLKFQDNEEISSYDLADAFKQMWEKKRYHEILFMVDTCQANTLYKRFNSPNILAIGSSRYGENSYSHHSDPELGVAVIDRFTYYTLEFFENVDPHNVTLHQLFNTYSPQKLQSHSEYRDDLFSRPLDKVPVTDFFGSVMRADITPSHEKKKQIITTEDDNISKYQKKSPNRVISLKNNEKSVSIKDKNSFQSKSPFENIPFIISLIGFFVLFSLLSWSSDYSKKILLNPDLIQFGKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMT5</accession>
    <name>A0A8J4PMT5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008647</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000554">
        <property type="protein sequence ID" value="KAF2070028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMT5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035252355" evidence="1">
        <location>
            <begin position="20"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="183" mass="20476" checksum="FF7015B1FBF47E9D" modified="2022-05-25" version="1">MKIIGSLLLLLATINLCMGDFTARLNFTRGGDRVSVTFDPNQESDYTTVLPTGGSMFTVEMPNYSHIGRAFIVSLDALDAKNSKPIDQNLVMYYESSGMMPTKSGVRISICGPNRRIFRFGLGLDQDINTNLVKMSIKPDPTRSCQYYSLPKYSYVNYISSDSTLLKPTLFIVVLLSAFFFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMW6</accession>
    <name>A0A8J4PMW6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Superoxide dismutase</fullName>
            <ecNumber evidence="2 5">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_009499</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the iron/manganese superoxide dismutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="2 5"/>
    <dbReference type="EMBL" id="AJWJ01000724">
        <property type="protein sequence ID" value="KAF2069184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMW6"/>
    <dbReference type="OrthoDB" id="239262at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.990">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="entry name" value="Iron/manganese superoxide dismutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019831">
        <property type="entry name" value="Mn/Fe_SOD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42769">
        <property type="entry name" value="SUPEROXIDE DISMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42769:SF3">
        <property type="entry name" value="SUPEROXIDE DISMUTASE [FE] 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00081">
        <property type="entry name" value="Sod_Fe_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Manganese/iron superoxide dismutase N-terminal" evidence="6">
        <location>
            <begin position="22"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="7">
        <location>
            <begin position="115"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2069184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="203" mass="23324" checksum="97C9485E4D29E0F4" modified="2022-05-25" version="1">MFSRIVVRVQRTAFADIQKRFKYTLPDVPYATTGISNFLSQNSVSEHLKLHKLDVERANNLIQRTPWENTTINQAIVQSSTSREDAPFFEAASSHFNHSFFWRSITDVKQTPSVYMKKAVEIDFGSFKDFQIKFSQNASALNVPGFTWLVFHDKALKIVNTFGNGSPLELRNCHPILCLDLFEHSYISDYQSNKNVNINIILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN87</accession>
    <name>A0A8J4PN87_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008517</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000532">
        <property type="protein sequence ID" value="KAF2070160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN87"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140860">
        <property type="entry name" value="Pseudo ankyrin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1095" mass="127515" checksum="E534A78DF6FCAD1B" modified="2022-05-25" version="1">MNNSNSSNNNNNHNNNLFYKIWRDNVLKRDVLRSIHTINITRVGYGTRKISNIKKYNEIHSLLWILDHGYIGLLFDKVLLNTSPLVFCREGLSKLFDIVNDSNEKINMFLNNNSSSSSGFKSISKFFSMFINNYKESLYNYSDILLDLIIKSNNFIAFSIIVTDMMNLNSDNRNNNCLHQLYISRKNICDAFYYGAFKILDYIRLHQSSCFKDWNYSTLFIISIGKRLNSGYIQLSNEMFRYLLFQSDPCNGADGGDSIQQLQFNCKSLNGIEKYFTISQLSQLDSNILVELDKCDFYKSLSSLDIQQFINEMDIFTTTPVILLNSLLIHYFKEKSNIHNNSNSCNSILNSIKNYWYNNKKIESVNSNNNDSNNSNNNDKNLIISILLNWKNEPKFYYYLILQQIKKIIVNQFHQNQLKQQYYSFLWKPCSQPNYYHINTIKVSILLYIEFKEQLDKDFIYNSVIDLTNNDNNNNNNNEIEQITILLNIFQLSLKYADIELFQLYIKIQDKIKLSLLLTRMTDKDIEMFSKSTASSLQIEFIKYFLKNSSSNIIELFLPIIINQLIIDNNIETVKKTFSYLLDHVETITINLNLFLNKQLLNSLTNNRDLEMIQLIHHQFGLFKNKNIDLLKKWPNQVLTMAIKNYNMVLVEYIVNNQLHLTTNTMALEEAANIRSFALIKFLMSFGFIVTSKVLLNCYIQGDVKSLLYLIKNTTVDCHELSSFISQDNLIQLLKNDFPLDFSSGRFGDLVFIYSDTLSIDQIDRGTVEVLYNELISNNTSYRNHTRALNIILELIQSNRVEYQTMILDLMAPSFFKNFLNIFLLNKMIAHALTSGNTHVMQAFLSHPIGANANVFSVVFENQDVYQKFFETNQIHMIKTFIDQLPSVFKIASFPTHSRYEHLVEYLVMNGHIGTLNFLINHFNLNLKHQNDNLLVSSIKSGRFHMINYILTLISFKDSKILKTLDIFKKYKESYSSDGSNNSFFYSFSDIDCLPEIFSLVSFRNNCNNSSHSNASNSNSSNIRIGDIQDDYYFNDHLEFTNKSPLSPKQNFNCFTNSSRNNKNSSGDAIYNQSEIYSKKDEIEYFLKNIPLSFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN92</accession>
    <name>A0A8J4PN92_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Lipase maturation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_008596</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lipase maturation factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000545">
        <property type="protein sequence ID" value="KAF2070087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN92"/>
    <dbReference type="OrthoDB" id="434126at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009613">
        <property type="entry name" value="LMF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057433">
        <property type="entry name" value="LMF1/2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057434">
        <property type="entry name" value="LMF1/2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14463">
        <property type="entry name" value="LIPASE MATURATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06762">
        <property type="entry name" value="LMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25179">
        <property type="entry name" value="LMF1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="294"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="397"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Lipase maturation factor 1/2 N-terminal" evidence="8">
        <location>
            <begin position="165"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Lipase maturation factor 1/2 C-terminal" evidence="9">
        <location>
            <begin position="406"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2070087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="591" mass="68728" checksum="C8C859B70439F325" modified="2022-05-25" version="1">MNSYIQRSINYIHKNQELVSKTFLALVYLICLKNYLQFIVYRVFISPTNDSFALTQWLLLRGLAVIYFISWISLKCQVLGLFGKNGIIPIEKTVTYQKRDRSSIWTSPSLFLFTNTISDNLLLNFCNIGLVCSILLFFDIAPTLNLTVLYIFYLSFKNVGEDFFQLQFDNLLLETGFIALLLPPFKVIPFLPGLEFGQVYHRSIWYLFHFFNIRLLVASGICKLSSGDVNWLNGKALDYHWWTQPMPLSTSYYFQKLPTMLKFVGCISHFLIEIICPILLMIPLFKSINSIALLTLQIMIMVSGNYGTFNLVSLLLGFSFFSDNDLPVVVVETIRDLVHISNYTIELPLFSNLQSWLNVYMALIGVAIVSFTVMMSFIPFSQVFRGIMLPPLSLYKVYMYIEPFGLLNYYGLFAVMTTSRKEIIFQGSIDGVEWKEYEFKYKIGDINHIPLFVLGHLPRLDWRLWFNQFHGFSINGEFWFHSFILKLLKNEKDVMDILSPKNKNMFVVDKQQKDSNSKITPPKYVRAMITPYKFSFDSSASRKELIRKDEQGVDQIWENGKWWSRKLPLLQYSPIAKLSDNGEKLVYKAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNU0</accession>
    <name>A0A8J4PNU0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009227</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000659">
        <property type="protein sequence ID" value="KAF2069454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNU0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="236"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="296"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="341"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="398" mass="43792" checksum="701FF1E5793210D5" modified="2022-05-25" version="1">MDNQNLWGNITNNNNNSTNNNNNNNNNSTPSSVFNTSPTPSSSIFSSSFSSSNTTGGSSFFSSFGQPQQQQQQTTTTTAFNSPTRTLSTPLTTTTATTLNPNSTPFEPKSFKNAAINYNNNNHNNGSIASAIGSTKNNMLFVTVPLSLFGDEQTLQTDLKKHFDKFGTSLISNVKRKKVFYTCSVTFSTIDEASEALKKGRSFNNSLLVIKPHKVFPGTTPFINNVTQTTTTTTTATPATTITPQPIQQQVKSNSNTSGGAKHDDVSLILKKDDGLMLRSQERQLRFQLMNANKSPAAAVAQPSPQIFTPPPPLPSYNSNNDRQQDNDDNDGDDQGGFIDNDNEQDFIDNDDVDDDGESSIGIQELDDQDYIDQEEQQPQQEEQEEEKKKRKKKVIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNZ5</accession>
    <name>A0A8J4PNZ5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008190</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000484">
        <property type="protein sequence ID" value="KAF2070492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNZ5"/>
    <dbReference type="OrthoDB" id="5855668at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04047">
        <property type="entry name" value="C2B_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037768">
        <property type="entry name" value="C2B_Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF138">
        <property type="entry name" value="COPINE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="152"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="321" mass="34558" checksum="0F54281CBC3FD23B" modified="2022-05-25" version="1">MSYNNYPGQYGAPAGGAPNLNKNSPSPGQYNLPPQQQAPPPQQQGYPPQQGGYPPQQQQGYPPQQGGYPPQQQQGYPPQQQQGYPPQQQGYGGYPPQQPQYPPQPQGYPGMNSTSYGAYSTTSTYGAYPGYPPQSYGYPAAPGYPPAPYGAPAPYPGVGVVAAPVVGVVAAPMVGEVNYILHLKGSRLDRKDVFSKSDPFVTISVPRNPLYAGKVKDSKKGGGSTANWSVVHRTETIRDNQNPVWQPFTVGLMAICGGNMEKPIKVECWDYDPNGSHDKIGSAVTTLREMQVMKEIRLVNKKRIGFGNTSGVIEILKCAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNZ9</accession>
    <name>A0A8J4PNZ9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007984</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000457">
        <property type="protein sequence ID" value="KAF2070703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNZ9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="91" mass="9932" checksum="CC438F339DE3785F" modified="2022-05-25" version="1">MGPYMVVLPNCANTAGPINALEYTVNIMANISKKIHPQNGTSLLCSNSTITPQSSDSVDTYCPSKSCNFSIPTQILFVHQASHLQAQNNYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPC0</accession>
    <name>A0A8J4PPC0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008112</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000474">
        <property type="protein sequence ID" value="KAF2070565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPC0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="18"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="707" mass="83414" checksum="C10A94CF700F746A" modified="2022-05-25" version="1">MGNRASNKKKTQIKDQLIIQRILQYVHVTDLFNTQLVCWRWYEISTSNHVWEAMIRKKLLGVLGDRSRVGPDQLIGSIDKLFYRTLYRQLFPHDYLLHLSHHLVFASLPLFINRGYGQDTEMFYQTVQKVPGMSMEEISKLKRADLLFFNDDMFENNYQILYDSPNAYYEQDEKRALLNVPRSCVYPAPTLDQDTKVITIVQDQHKFHYGRYGNDNRDVIVDSIRKQTTFLIPNENNQYTKAVVVRYIEANYDRPSRGYFDDNEGGIEMVGEVSLLNYDLGQPGKEKKRYREEKKEEENDEEDDQKEGYGFTPEKTTLKVFGIVSNIFNQDPDGQDKSPYTNNCTIGSGDEETDIGIDVGSREFYVSREKIQQIESFCNGSLLGKTQRDLVFLLQNTEYVELLQEFDFNSISLDQDTMYKLGDIKFFLEYSQRNPLQIQFISFNMEIKFRMVIQMKRVCFFCSDICHVDNYNPDCPMKNCEESVQGRESLYNAFVQIYFYKFVSKYYREMENPGFQHYMFDTEFSIPYCTNTNSNTPDKMVYFKITFGSQDPLPDRDHYGDDPFFYFEFKIEYSMVDINDAKPRSQHSFSTLYIRDQDKNKPQANESFMDGVRESFENVFPNFKFDIILLLKAFKLDLYFIFAFQTNKKVYKSSPFLQLRIYASYKSLFEFECDSGLGLMTNIYCAIDEEDWNCKDDPKYLSSRCNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPH9</accession>
    <name>A0A8J4PPH9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FAD-binding FR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_006837</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000333">
        <property type="protein sequence ID" value="KAF2071845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPH9"/>
    <dbReference type="OrthoDB" id="1856718at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030586">
        <property type="term" value="F:[methionine synthase] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050667">
        <property type="term" value="P:homocysteine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.990.10:FF:000007">
        <property type="entry name" value="Methionine synthase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000001">
        <property type="entry name" value="NADPH--cytochrome P450 reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF84">
        <property type="entry name" value="METHIONINE SYNTHASE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="8">
        <location>
            <begin position="60"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2071845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="507" mass="57554" checksum="8911FD26FB740D6C" modified="2022-05-25" version="1">MSDQSLDSTSQPPIIDKPVAPVKKKIVLKRAAGTANANHVLRIKVDKIENESIKNVVSLATDQHATIECAKYLTLFKKDTAESSPTTTDIDIKRVIEITLDTNSSTISYQPGDYIQIFAPNPHNLVLKVLNRLGLDKNQFISIESLDLLKYELPNNLKRSNNQKLYYIFRDMLDITGIPSKKLLKLLSEYSSDQNEKNELLKLSSVEGKEEYSKLISTRSNLISLLDKYKSSQPPLDYLLDILPPLSPRDYSISSSPLVIENGKLNFVFSVVDMIIGNERIRGHCTSWLESICLNTNKIKRQDNNSDDDFLLEIGLDSLCLDNNEKPVIPIMFKSSPHFHLPEDLDKPIIMIGPGTGVAPFIGFLQHISKQLKFNPYTWLFFGCRSEKRDFLYKDTILDMVESKTLKKLTTAFSREINTTSTIDLSLDITCGYVQEKMKNHSKEIFDLVYNQNALIYICGDAKGMAIGVRNSFIDIFQNELSISEKDATDIFTQLIKDKRYLLDVWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPS9</accession>
    <name>A0A8J4PPS9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_007395</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000392">
        <property type="protein sequence ID" value="KAF2071288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPS9"/>
    <dbReference type="OrthoDB" id="19313at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097039">
        <property type="term" value="P:protein linear polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23820">
        <property type="entry name" value="RWD_RNF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051628">
        <property type="entry name" value="LUBAC_E3_Ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770:SF13">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770">
        <property type="entry name" value="UBIQUITIN CONJUGATING ENZYME 7 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26200">
        <property type="entry name" value="Rcat_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="229"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="69"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2071288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="525" mass="60714" checksum="4B93E171FD210AA7" modified="2022-05-25" version="1">MDSLEDGYQEIQNQEIEVLKSMFGESFYQTVVNNSDDQDDSLSIRNNIKFFVNVPEGFSIATFNSLKDSKDNNNTTTTTTTTTTTTTTSTNDNSTKEDLKLYPINHLPFVELSFEYTKNYPDSCPKIYLSCSWLRLDQMYAVVKKLESEWSMGEFVIYKYYDWIKTEMFRYLNIEQYQLFTNEPMDFQQESSDQVLWSCQDDWVSLLSALPILLQHNLHESKRSFFMEPQHECPICYCEYPSDQFILLNCLHYSCQDCTSNICKINIQSGDIGLLKCSTIGCNQLFDQSYIKSVVTANEYEKYETYLQKEKGLFKCTRCKDGWSFIDNYTRSSFCTKCHYSSCTLCNGEFHPGLPCPNMVVVSGDNQPIVAKQALKRCPSCGMIIDRYEGCNKVVCRCGCTFCWVCLKTIKGYDHFQQVCELFGMPKVRGTNTKRATIVYFSDYTHCGRCKSMVLRANKNNHCFCTQCRQTICFICKKFIKGTTHFSQVGSICCQHGDPPNHTYDQSYIINSNAKTNYYYDEIGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPZ2</accession>
    <name>A0A8J4PPZ2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006794</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000329">
        <property type="protein sequence ID" value="KAF2071892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPZ2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="75" mass="8127" checksum="2A4DAF105F2281CA" modified="2022-05-25" version="1">MIFNTISQIANPSQSMMKSSTKGGASMDGASANNQLSRRGCCGGRGRRNDNIDICIDIDIDIDIDNNNNRCGNWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQH4</accession>
    <name>A0A8J4PQH4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_006402</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000293">
        <property type="protein sequence ID" value="KAF2072295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQH4"/>
    <dbReference type="OrthoDB" id="18650at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17330">
        <property type="entry name" value="MFS_SLC46_TetA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF111">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="116"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="340"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="412"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="440"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="509"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="114"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="61"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2072295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="550" mass="60314" checksum="CEA1D1F3EB3E2BF8" modified="2022-05-25" version="1">MGKNDNGNTPILECESEPEDEGPHHPQNQRSGSVSMIILNSPTLHEVVDNPKSSLISTTNDDTDYSEISDDSTESDNQSVKEEDLESIHIEKDDLLSNDADAKPPPISKSQWARIIMIYMAVFSDALALTLVTPFLSEILIEKWNIQEDNVGYYSGILVGIYSLARFISGFNLGHLSDKYGRKPFLILSLLATGIGTIIFPLVPNVYLAIIIRFVEGILSNTMALCQATLADMVDKKNRPVVFAYMGSVFALSRCLSSAIGGFSVKIAQDAKNPYLLPCIIGGSIVLLSGLLILVFHPETHPKLAKKSITEETVEPTLNVEQKEYSFFEGLKLITKNRTVLHLMVMGSLNSFANGGLLLGIVLFASLSIEEKGMGFDSFKIGILFTVFGLVGFAFQLLFFKRLSKSLGLKKQYLMGMILLGASMLLYPVIYTGYLIQGEAMVWVISIFVVIIMGIGWMQAMSILQGMVANASDPKIQGLTQGSYQSLNSLLRAFGPVVSGAIFSLSTNWSAPFILFIVLAVIYLTVGKISHHLPESVDVLKKKEVTQEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQP8</accession>
    <name>A0A8J4PQP8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_007521</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Golgi apparatus</location>
            <location evidence="9">trans-Golgi network membrane</location>
            <topology evidence="9">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the syntaxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000406">
        <property type="protein sequence ID" value="KAF2071166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQP8"/>
    <dbReference type="OrthoDB" id="546861at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048193">
        <property type="term" value="P:Golgi vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21443">
        <property type="entry name" value="SNARE_NTD_STX6_STX10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15841">
        <property type="entry name" value="SNARE_Qc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.90:FF:000004">
        <property type="entry name" value="Syntaxin 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015260">
        <property type="entry name" value="Syntaxin-6/10/61_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF124">
        <property type="entry name" value="SYNTAXIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09177">
        <property type="entry name" value="STX6_10_61_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="233"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="163"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2071166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="259" mass="30226" checksum="3A4FDDC6F43FF448" modified="2022-05-25" version="1">MASADQDPYYLAEQEVASSVRGLMSLHETWKNLIFNTNTYTDKEFRWTSRELRKLIATLTENIDDIHDTIKICEKFPAKYNISSDEIEKRKSFVRDVRAKIGEVTRDFDDPQYNRKIEDDKSKELLSNERRTNIEKSSRFEGLRRAHEEDNNDFLREQAGMQQKLIEEQDQGFDMLHDQVRDLGEMGKAMESELKLQGGILDRLEHRAMKSSATLASVMRKLDRFMEQTSSKLQWTIIAILAIIFIILVVLSSTILKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR94</accession>
    <name>A0A8J4PR94_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_007104</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000361">
        <property type="protein sequence ID" value="KAF2071571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR94"/>
    <dbReference type="OrthoDB" id="6614653at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050218">
        <property type="term" value="F:propionate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43347">
        <property type="entry name" value="ACYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43347:SF3">
        <property type="entry name" value="ACYL-COA SYNTHETASE SHORT-CHAIN FAMILY MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="4">
        <location>
            <begin position="16"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="2">
        <location>
            <begin position="82"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="3">
        <location>
            <begin position="524"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2071571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="645" mass="72372" checksum="349C1085DBE2F1E9" modified="2022-05-25" version="1">MIENEQQQYSSEPFNYDHDYKQLVDTPLEFWNEIANKFVYWDTPFVNVMGGTIEYPLWFEGGALNACYNILDKQALNPDIKNRTALIHTCPYKGIEEKLTYSELYDRVCEFSTALRSIGIKKGDRVLIYMPNINQTIISTLTCFRLGAICNIVYGGYPSHQLAMRIDSFKPNLLLSSDFGIYGTETISYYGLINNAIELSNHKVDNIIIYSRKDLVFNHTDSIPEGSLDWYELINKSTNQFTSINREYTPVESTHPLNISYTSGTSSQPKGIVKSTAGTLVAIMYYLCNNIALNKVPGVYFSKTDFGWETGQIGMVYTPFLNGLTSIIYEGSSVEPKKGLWSVVEKYKVNYLMICADLELLMKYDDSHLFNLSSLRALMTGGRKVLESTSIFYKKITGLHLIEVYGSTEAMSIIGNSTRTGVTQLGVTGFPMPGFSLVIVDENSQPVGNGEAGELVIKLPVGPGVATTIDNNHQQYIKSYLAKHKGYFATGDLAIKRDDGYINVLSRLDDIVVVNGIKISMEPVEEAISSHEAIAESIAIVIEQPNQKDYTIGLVVLKSIFLNKDKNNIISQIGQLVEKKVGGLPNFKTTLFIERIPKNRNGKTLKKMIANIFNGKSFEIPSTIQEEGQDIIQEIQQIYKDYCMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRF9</accession>
    <name>A0A8J4PRF9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">IPT/TIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_010459</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001127">
        <property type="protein sequence ID" value="KAF2068216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRF9"/>
    <dbReference type="OrthoDB" id="24520at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052014">
        <property type="entry name" value="Dictyostelium_Tiger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31341">
        <property type="entry name" value="IPT/TIG DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035307529" description="IPT/TIG domain-containing protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="4">
        <location>
            <begin position="531"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="4">
        <location>
            <begin position="623"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="764" mass="82327" checksum="587F077C8981D5A2" modified="2022-05-25" version="1" fragment="single">MKATSIVVIVFFAFIVLSVNAVAPVIDSLILSWGRVGKTISIIGSNFGTETPTVWVDGLPAATNHISDTVKSFTMYGSDTARQWTISVSNLVDEMSNSLVLFAGGTNPDQFYQINEKYFIVGDFGQIPLKDSLKVTRNGNDFYGVTYINSTHMSFNLILDLAQSPFKLYYGSYLEGTYNTPGQYAPVIDSSAFTNQGLSIGGYVFNQQTVVKTGSTFTSTCKITTQTVINIYCVPEARSLLNPVNQFQVTLFTGKQTTIPVTFTHLQSFLNDINNICILKLAGFQNNLGLPTQIDGLGTVSAISASLVSISEIKFTYPLDAKCGYAFVFAGTTRLTNNMLLCPRSVISTVDAPRADNGYVLTVNGYFMNPYLFDPTSTLQVQALTFSYVYADGITHFCPVTQVVWQPSNSSYTLTCTIGTPKAFKFYINSTVAESNFFLVGKDPIVISATPTDYLVPGVVTIIGDLFTSQPKVKIGNVECIPATLVNNTMITCMFPSNVAVSNFKNPLSVQVSLDLGFTSNAFVFLYNRPNPVITGATSTIYGIAGKVTVTGFYFYSENPVFVSIGGVECLFQSGNGDGTGIVCTFPSTAGSQYDYDTPLSVHVSIDNTYNATTAVFYYFRPNPIITSSTSTTYGTPGQVTITGNFFSSKGLVVSIDGTPCTSPTVIGVTQIVCTFSSNTVVSNFKTPLAITVKDSLYTVTNSVFYYVRPNPVVSSATPTKYLSAGQVTITGKYFSYPDSLSVTIGASICSSPVASQDGNTITC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRI8</accession>
    <name>A0A8J4PRI8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">Nicotinate-nucleotide pyrophosphorylase [carboxylating]</fullName>
            <ecNumber evidence="5 12">2.4.2.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">Quinolinate phosphoribosyltransferase [decarboxylating]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_005705</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12">Involved in the catabolism of quinolinic acid (QA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>nicotinate beta-D-ribonucleotide + CO2 + diphosphate = quinolinate + 5-phospho-alpha-D-ribose 1-diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12733"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 12">Hexamer formed by 3 homodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the NadC/ModD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.19" evidence="5 12"/>
    <dbReference type="EMBL" id="AJWJ01000234">
        <property type="protein sequence ID" value="KAF2072993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRI8"/>
    <dbReference type="OrthoDB" id="10067394at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00331"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034213">
        <property type="term" value="P:quinolinate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01572">
        <property type="entry name" value="QPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000090">
        <property type="entry name" value="Nicotinate-nucleotide pyrophosphorylase [carboxylating]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.20">
        <property type="entry name" value="Quinolinate phosphoribosyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004393">
        <property type="entry name" value="NadC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027277">
        <property type="entry name" value="NadC/ModD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037128">
        <property type="entry name" value="Quinolinate_PRibosylTase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022412">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00078">
        <property type="entry name" value="nadC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE [CARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179:SF3">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE [CARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02749">
        <property type="entry name" value="QRPTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006250">
        <property type="entry name" value="NadC_ModD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54675">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 12" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase N-terminal" evidence="14">
        <location>
            <begin position="39"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase C-terminal" evidence="13">
        <location>
            <begin position="116"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR006250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2072993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="290" mass="31958" checksum="073B86163B31086A" modified="2022-05-25" version="1">MDPSLLFPPNAIQTLVRQWLDEDIPSFDYGGAVVGNDIKTAHLLGKQRGIFSGAPFFNEIFTQLNCKITWFIKDGQEFNLDSNGKPFVLAHVTGPSRNILVGERLSLNILARASGIATRAKEMRDLVDQVQWKGKVAGTRKTTPGFRLVEKYSLLVGGLDTHRMDLSSMIMLKDNHIWSCGSITNAVHSARSLGGFSLKIEVECQNKQEAIEAIDAGANVVMLDNYTPDRLAQDSKELKQLYPHIIIEASGGITPSTIQQYALPTVDIISMGNLTQGVPHIDISLKIQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT23</accession>
    <name>A0A8J4PT23_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TPR repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006187</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000274">
        <property type="protein sequence ID" value="KAF2072500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT23"/>
    <dbReference type="OrthoDB" id="1924189at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26312">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26312:SF87">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="81"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="156"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="29695" checksum="C39C97FD43F443BE" modified="2022-05-25" version="1">MGCCCSKTKEDKEQDNELNKRDLRTSGSKGKESVKNAKTNGGANQIDKLGPKVNTTLAFESVKVHEKQEILDSLDDNPNDTTAMANYSIILAKEGKMKEAQDLLTKAIEIDKENSRVWQCYAELYERQNNITKAQEIYNQGYKFASPKIALGDDDSNLMLNYAISLQKSKNYDKAEKLYKRAVTSGPGNPETAGRYGSFLLEIREDIDRASQYLKQAADFTPPHRGWCEYYAKFLKDYEKNDQESTKYQKRATLYVASPSTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT36</accession>
    <name>A0A8J4PT36_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004602</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000166">
        <property type="protein sequence ID" value="KAF2074073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT36"/>
    <dbReference type="OrthoDB" id="20650at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="161" mass="17749" checksum="DE2FCF733DEFE3EB" modified="2022-05-25" version="1">MSSFGNRGRGGRGGRGGRGGRGGRGGRGGSNSDFRIGGNVSEEQRKYFTDASQEIKDLGLKVTAKRVKKDIEKEKLAASGRKIKKTYVPFKTAITKHKIDKEKQDQLISEAIMMGEKPRLAKKAKEEREVSSGGLQLKNFRDGVLHIKKSDVRSVFKGKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTR3</accession>
    <name>A0A8J4PTR3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">UbiA prenyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_009387</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Quinol/quinone metabolism; menaquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000695">
        <property type="protein sequence ID" value="KAF2069296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTR3"/>
    <dbReference type="OrthoDB" id="203513at2759"/>
    <dbReference type="UniPathway" id="UPA00079"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042371">
        <property type="term" value="P:vitamin K biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026046">
        <property type="entry name" value="UBIAD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929">
        <property type="entry name" value="1,4-DIHYDROXY-2-NAPHTHOATE OCTAPRENYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929:SF4">
        <property type="entry name" value="PRENYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0474">Menaquinone biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="144"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2069296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="245" mass="28243" checksum="0CE01D6ED4B47723" modified="2022-05-25" version="1">MFMNATTIKASNTKLDSITLLFVSICFKMNIVKKYLIATRSFFNITCFVFLLEGIIVSYVQTQSIHLGKLVLVFLLSVLTHNMSYLANSLIDFCTGSDVAETSNDRTLFELITFKELYLFLVFISCLICSLLFIVYNVFSVIEFYQFLYFVIFQVICAWIYTPLKYFAMGHIIFSGFFMSVYFWAIFVCTGSFPVWGPEVKYSLLTPILLTLAIHGNYIRDYDADKKAKIYTTATIFKKKKCNIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUC0</accession>
    <name>A0A8J4PUC0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HIT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008994</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000614">
        <property type="protein sequence ID" value="KAF2069686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUC0"/>
    <dbReference type="OrthoDB" id="672793at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23089">
        <property type="entry name" value="HISTIDINE TRIAD HIT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="2">
        <location>
            <begin position="12"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="122" mass="13497" checksum="FFE800BF382457E8" modified="2022-05-25" version="1">MASSKEIAENSLFGKMVTGKISCEKVYEDDYCIVIKDISPVAPVHLLILPKHAVYSVSCLSNDSEQDKTAMGYIMTIIPRIASILQIKSYRLVINEGEQAQQNIGYLCIHLIGGRELAWPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUQ4</accession>
    <name>A0A8J4PUQ4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003461</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000108">
        <property type="protein sequence ID" value="KAF2075243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUQ4"/>
    <dbReference type="OrthoDB" id="413993at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053044">
        <property type="entry name" value="Metallo-hydrolase/TatD-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47345">
        <property type="entry name" value="CUT9-INTERACTING PROTEIN SCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47345:SF1">
        <property type="entry name" value="CUT9-INTERACTING PROTEIN SCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="140"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="345" mass="39374" checksum="283AB9048DF409BD" modified="2022-05-25" version="1">MTPMEDQQQQQESEQKIEFDLHKHDIIDTHCHIQEDTDSIEKAVALPFAKSWLMGTTVADWDRVDSLANAFEQQQRGDHVLRCFGIHPWFVYQYHPPKELLGLPITTSLVQVKPKEGEGQLKEAEGEGEEEEESGKCSCTSNDNTTSSNSNSNEIYKIDNTWEEKMKALLEKYPEAMVGEIGVDKVTKVKATGKNEQEAQWLFLSKQIAMAKQYDRLVSLHCVQLHGKLLDFFMALPIDQFPKRIALHTFGGKPSTVSQFSKMKDTKGDRFYFGISFINLTSSRIEKLIQAIPDDRLLLESDQNTPLEAEQSLFRVIQTVSKSKNWSIEATIQKTKLNALRFLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUS5</accession>
    <name>A0A8J4PUS5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ribonuclease Z</fullName>
            <ecNumber evidence="4">3.1.26.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_005191</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.</text>
            <dbReference type="EC" id="3.1.26.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase Z family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.11" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000201">
        <property type="protein sequence ID" value="KAF2073482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUS5"/>
    <dbReference type="OrthoDB" id="527344at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042781">
        <property type="term" value="F:3'-tRNA processing endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990180">
        <property type="term" value="P:mitochondrial tRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07718">
        <property type="entry name" value="RNaseZ_ELAC1_ELAC2-C-term-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047151">
        <property type="entry name" value="RNZ2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027794">
        <property type="entry name" value="tRNase_Z_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553:SF63">
        <property type="entry name" value="RIBONUCLEASE Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23023">
        <property type="entry name" value="Anti-Pycsar_Apyc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13691">
        <property type="entry name" value="Lactamase_B_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNase Z endonuclease" evidence="11">
        <location>
            <begin position="11"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2073482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="707" mass="80276" checksum="6050CB1EE4581349" modified="2022-05-25" version="1">MTKSYIDVTGDNEDATPILFANFDENRYLFEAGEGVQKVIRDLEAVSLPKVKAIFITSLSWDSIGGLIGLIFSLSDAGVESINMYSPKGLFKILKASRAFSCVPSLKIHLFELNNHLSHHFKVSNMNVTSIPLYKTLPTFKDVKMSRDIIVQNHYNNWDYEQMNQEDDYSENQVDQDAIVCYIGSTPDLPGKFHPDKAIKLGVEKGTNFSLLNSGQSVISKTTGLTVTSDMVKDPDQPGTRFALLRCPSKEYFNSLFNNDTFESFYSGKEKLCVVYHLVPESIITSQEYQQFIEKLSFENKNIKHILVNSENCERYPSYPSSDHYVKKLKALVPPLFQLQDKKQNDRQFNKLDIKNVISCEKVTRIFMAPLAKVGHLEIRPYQTDIHDIWEFHHHQDSYPEFKQEAQKVQQEIQRIENIQDKVQYPKVLFTGTGSAVPSRFRNVTGNYIALSEDKGLLLDAGECTYSQLYRSFGQDKIKDILLGIKLIWISHEHADHHLGVARVLLKRSQYTDEPLVVAGPPRFISWLTKLSVCSPNINFIGASITEKSEHLDQALKELDIESLVSIPVIHCDESVGVCIQLNSGVRIAYSGDTRPCPEFIEACKDSLVLIHESSFEDDQLEDAKSKNHSTIGEAIQVGRDMNAKCTLLTHFSQRFCQVQPNPPETIVNTEEKPFAAVFDFMYISPYQYSLLPHITKAINILLSEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUZ2</accession>
    <name>A0A8J4PUZ2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004693</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000171">
        <property type="protein sequence ID" value="KAF2073986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUZ2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040405">
        <property type="entry name" value="DDB_G0275255-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34411">
        <property type="entry name" value="DUF6748 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035216211" evidence="2">
        <location>
            <begin position="26"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="34"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="106"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="155"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="314"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="338"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="657" mass="74756" checksum="70BA6C0F4CA85B2C" modified="2022-05-25" version="1">MLSSSIKSLFVVIVLIVIIFNDVKAATPTPTARNNNSNISNKNLYNNNNNVLNNNNNNNNINNNNINNKNNDKITNNNYNSDNIINNNNNNYNNNINNNNYINNYNNYNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNYITSTPKNVDLKNNNSNNNTVNKNITITSTPSLNKNNTIITNMTGNSNTTKIAQNNNSNNNTINNNNIANNTNINSNNINNNLNNTKLNNTKIKNSDDIINDDINTNKNSNNNNYKQTQKNNNTSIKEESNNDNSPTDSFNTTNNNYNNNNNYTNSNYTNNENKSIIIEETNNGNNTKNSSLKNPDEIDREQNEKNSTTISNSNNNNSNNNNNGNQSNNVENESKAKPCTDKERAPPIIPSHYYMIKPTWGFNRKTYEIRLATEYDSDDRDLLMIERVSTISIPFLDFTDHNITALLDNNNFEVMVKGYVTGSEVLGYTLHVVDNELYKMIPIPGIEFDFLYQRYYTLDQNNQYLESIPSVDIYNISEVNSGGKNNLSVNGPNLKINRILKKIPSIQQTWVEYRINSGNAIITRNRNIELDDPNFEVEFVFSRIPEDIVCPKVLYAVHCNPDMTESYQRTMKRCVRFSGCVKKTECPLLAPKEKQCPKGFTPVILPRKPSGCDEITCDPDFLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PV78</accession>
    <name>A0A8J4PV78_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Maspardin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_004420</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000155">
        <property type="protein sequence ID" value="KAF2074287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PV78"/>
    <dbReference type="OrthoDB" id="10264550at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026151">
        <property type="entry name" value="Maspardin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15913">
        <property type="entry name" value="ACID CLUSTER PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15913:SF0">
        <property type="entry name" value="MASPARDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="5">
        <location>
            <begin position="87"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="277"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="277"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2074287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="316" mass="35910" checksum="F6BD8FAFFA6C57A4" modified="2022-05-25" version="1">MNSNKKDVYQWFRSTIPQQRISIGSVNEKVWKFYDYGPKDITPLVFISSGTSEIHYKQFVSLCPKGYRCISIQFSPYDTIQGWCRGFDRFLDRLELDKVHLFGSSLGGYLAQCYYQQKPSRVLSMILNNTFSDTQYYHDNSSCSAMFSILPEFMLKRIILSNFPQGLLDADIAETVDFMVDQIENLSQEELASRLTLNCSPSGVINPSVGLENITIIDSLDATSVPERLREEVYKYYPQAKIALLKTGGDFCYISRSDELNVHIQVHLRQYGLDPVNNISNSNNNNSNSNSSNGDGNHSFEIESKGKSIKESDIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVC2</accession>
    <name>A0A8J4PVC2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Coiled-coil domain-containing protein 86</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_003056</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in nucleolar integrity and required for processing of the pre-rRNA for the 60S ribosome subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Required for proper chromosome segregation during mitosis and error-free mitotic progression.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CGR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000091">
        <property type="protein sequence ID" value="KAF2075633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVC2"/>
    <dbReference type="OrthoDB" id="277961at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026570">
        <property type="entry name" value="CCDC86"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005579">
        <property type="entry name" value="Cgr1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13557">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 86"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13557:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 86"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03879">
        <property type="entry name" value="Cgr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0164">Citrullination</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="227"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2075633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="279" mass="30666" checksum="53B26610A9D666CD" modified="2022-05-25" version="1">MTQTRRTSTVNNLDLEKAQQTIVKKRRVGSTPVKAAAPPPSESESSESEVEETPKPKITRRKVAAVAKPAAVAPAAVAPAAVAKPAKAAAKRRVATKTVVAVEKENTQEEPVVIADEATPAIIETTPAVAQVETTTPTTVTIIDTPTIKTEAQEEATTQTANASASFRRAIPLSQGKPVSGRVWKQPSQKFHKMINVKSLKTTFEVKEQARMERKRIKDKENEIKQASRQAKTNMYNEMKENKKIKEANTKKSEVVQVIKNTAKIKKMNKKQLKLIRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVE4</accession>
    <name>A0A8J4PVE4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_003941</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000131">
        <property type="protein sequence ID" value="KAF2074753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="427769at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057709">
        <property type="entry name" value="DUF7949"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25820">
        <property type="entry name" value="DUF7949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1315"/>
            <end position="1338"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="6">
        <location>
            <begin position="403"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="5">
        <location>
            <begin position="617"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="4">
        <location>
            <begin position="763"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7949" evidence="7">
        <location>
            <begin position="1024"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="3">
        <location>
            <begin position="1082"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="986"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="986"/>
            <end position="1010"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2074753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1361" mass="150961" checksum="91A794DB3D3A10AE" modified="2022-05-25" version="1">MQDITPPSYLNKYSTASATGQCQFVYSLVFTQIINEGPITVPITSPIQLASLNYKSTNNIQSFYDISVDVPIGESQFFVGATSGAVTKQFLISYTCEAPPTTLDITFFNDTLYDSYGPSTDGPILTFKVNNFLKTGPALSATSSSQSLIVDQNSLILLSDNLYGLALKFSDGTKWTDIENDFTISIEYKGSTFALPSNHLIKSNIKINNQIQIGSYTLYSLNPLNSTKSYHLYPYIEYQSSLDEHPLWVMTSSVNAFNSRLSLSMGSFSNGLITSVLYKGGVTTTSISLSNNNNPVPIISKPVTFQPPPSTDTVYSSSTDLTNGILSSILSTNMPIKQCLILSGLPTPYFYYSDSFYPYGITQGTSLSHIRQDEFFVSPLKKGIVYVSYPLTMNSYELIPSIYDDSPPNIESIETFYIGSILIYRVHITDDSSGFFQIDKIGNYTSLVSGTIQDGIYEFQLNIAEVFFPTTVLLIYDKALNCKVVDMTTFQTAGKHSFKPNEFNSVLDINRVYWKYNDVDVSGQAVDNILYICSNVTTLKPHILPYNQATTLIWYRPVLGIYNSSIGCYEYEYPIHKNFIPGSYDSYIWLGSLWRSINFLIPMFGEESKLRVRSTFGDALGPLIGSITLLPSGSVLIQSDTEIKWKLTISDPINGFKTGIVSIVSQVGWVRYNFTLSPTVEGHMVSGDQYMGFYEISIPINSICTSQKFSIYFIQLEDQNGWISEHPTTNIFYINPLMKYIDDIDATTSISIDCPSSTDISAPTLSSFDFSPKWIDPTYSGELFSNTPRMVHFQFQVSDNVGILKEALPVIYLSDQNFNRISQISELVTYSTTYATYECKFNIPFGYGYPEGIKVSLFGLVDFNNNIGGYTIASLNSTFPNTIEVSNTFSPKISILSTLPLYSDRDFYVMGTNFQSTDVLMIQDERLDIISNISSISSMKTISKFTMGALQGDFVFITFRREVSNGVYEYSNSFKVKIMGTSTDSSSLSSSSSSSNSKSSSVPASSSDSSPETIPPTNAPQQCIDQCSGNGECTLNGCICKSPWVGLDCSSKVIIIDPSINSTIPVTNVTIPPTNNGESVLYAIISILSLNELNNNGDIINSYPFTKWVASNNTNSKYSEYTSLSYLYTTTVTNKYDQSTTTLNVSIDYFDKDAVNITFANQILTMNPYSLKYSVNISQYSFSSSLNTLQVVLSASIESEKDDNECSSIQTGNTVESESEFIKIQVNDHSLYGRFIKRGIVDGRIRQITNTPLNTYSKSTPSKSQTLIGINVPHYQKMIQLDPDFSVLLDNRAATIDSPNAVCFEKKSKLTGAQIAGIVIGSFALVAIVVVCIVYYLYKRKQKAIFMKNIDQKLGQINNKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVL4</accession>
    <name>A0A8J4PVL4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_008231</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000490">
        <property type="protein sequence ID" value="KAF2070451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVL4"/>
    <dbReference type="OrthoDB" id="433924at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051219">
        <property type="entry name" value="Heterochromatin_chromo-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22812">
        <property type="entry name" value="CHROMOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="4">
        <location>
            <begin position="788"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="479"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="628"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="679"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="19"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="42"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="79"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="490"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="651"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="680"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="693"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="758"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="768"/>
            <end position="782"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2070451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="849" mass="98730" checksum="E2CF58E56A34EC5D" modified="2022-05-25" version="1">MSSNNNSPLASSLRLNLSTNTDDSNRTIINLESPHSTAMSLNNNSGNNNNNNNSSNNKQQDDVIVIPDSPKDDSSHMTSSNNNNNKNNSNNNNSYSNNNDKPVQQTSPTQQSIQKQTIQQQQQQQQQQQQQQQQQQQQQSAPRIVNNFNSSTATLTPGQILNTLNSLTSYSQFIQLSNYVSTLRVTFNETLQEAHRNSCLERIHIPKTIEETVSLITICEQPKQYYDMFFVSSLCTQPTLDKSYITLKTLLVNHPALITKINEAYKLLNTKEKRRAFDAAHIPSHTAKCLCCDKSCLINQANIESFLNRTLASCYNCKNNFFYYSCDSCGGGYIMCEKFVNGYCAKCIICDSIIYSYLKCPSKDNTDSLKIIQLYYKKLTESQQKAQQQQHHQTLQQQQQQQQILQQQQLKQSQQKIAQQQQQFVFQPNPAQQLYYKQTLQQTAALENQKYAQFQQQYQYQQQRQEQLQQQQRQEQQKIAQFHQQQTSMLQNQQKPLQPQLQQSQPQNIPPQPTSGGLKKAHSIIKPPAKIAPTTTVVPPTITPTANVQMNIKPPAKVLTKEELLVLEIKEIERRVRLKVHEAWAEFNLIGARRRKKLEATTNKLTKPGTILFLKWHPEFHKKEMERIKASRNQEKKRKREIQEANHKKSNSSGRINNNKIPQEKKKVSVDIFEKIPKTASNSSASSSSLKSKNVDIEKEKKEKREQERQKERERERQRQKQKEREKQKEREKQKQKEREKGRDKKEKEEKENEKTKRLIKQKLKDKKIKEKEPVPEKKKDDSDEDVYSVESILDKKVEDGQVQYLIKWEGYGIQDATWEDKENCFCEGLIAQFEKSFSTNTVRHHYLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVN9</accession>
    <name>A0A8J4PVN9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_004629</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000167">
        <property type="protein sequence ID" value="KAF2074060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVN9"/>
    <dbReference type="OrthoDB" id="10250002at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000495">
        <property type="term" value="P:box H/ACA sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031118">
        <property type="term" value="P:rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02572">
        <property type="entry name" value="PseudoU_synth_hDyskerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21148">
        <property type="entry name" value="PUA_Cbf5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.10:FF:000001">
        <property type="entry name" value="H/ACA ribonucleoprotein complex subunit CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012960">
        <property type="entry name" value="Dyskerin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004802">
        <property type="entry name" value="tRNA_PsdUridine_synth_B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032819">
        <property type="entry name" value="TruB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00425">
        <property type="entry name" value="CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003280">
        <property type="entry name" value="PRK04270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127">
        <property type="entry name" value="CENTROMERE/MICROTUBULE BINDING PROTEIN CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127:SF0">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT DKC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08068">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16198">
        <property type="entry name" value="TruB_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01136">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dyskerin-like" evidence="5">
        <location>
            <begin position="29"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="PUA" evidence="4">
        <location>
            <begin position="278"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="411"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="411"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="423"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="441"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="503"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="514"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="524"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2074060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="556" mass="62808" checksum="EAC73316442175A1" modified="2022-05-25" version="1">MSDKKKSSSSSSKEEKDYQIKPEKTPLLDTSKWPLLLKNYDQLLVRTGHYTPIPNGHSPLKRPIKEYVKYGIINLDKPSNPSSHEVVAWIRSILRVQKTGHSGTLDPKVTGCLIVCVERATRLVKSQQGAGKEYIGIVRLHSAIDGTHELSKAVDTLTGALFQRPPQKSAVKKRLRVRTIHQSKLLEYDPERNLGLVWVDCEAGTYIRTLCIHIGLLMGVGGHMQELRRVRSGIMSEKEGMVTMHDVKDAQYEYDNTKDETYLRRVIQPLEAILTNYKRIVVKDSAVNAICYGAKLMIPGLLRYEQDIEANQEVVLITTKGEAIAIGIAQMTTASMATCDHGVVATIKRVILDRDVYPNRWGLGPKAQKKKAMIKAGELDKYGKPNEKTPKEWTNTYQYYTGSTDAAPAAAATTPSKAAEPVKAAKEEKMKSADSSDEEVVEKKKEKKEEKKEDKKSSKKSKKDDSDDEKMKESSDDEKAKKKSEKKEKEEKKEKKSSKKSSKKDDSDDEKKEKKEKKEKKSSKKDDSDDEKKEKKDKKRKSSEESSKDKKKKKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW62</accession>
    <name>A0A8J4PW62_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Importin subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_003968</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the importin alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000132">
        <property type="protein sequence ID" value="KAF2074736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW62"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="438"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="443"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="485"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2074736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="495" mass="54195" checksum="A78FEF1C0291C0DA" modified="2022-05-25" version="1">MDETIPDATEFNEFLKLFNSTEIQDIVKGTSGLRKLLLYERCTPDQVLGATVLPRLVAFLESSSPEDLLYETCWMMSIFAFLQYIKEIVGTGLLVPLVKLLSLDTTSDRVRDKITFCLGNVTKQSLQCRQQLLELGIMKYLIEIVENTSDQQLLRNSAWLVALLGRGPYPPLQMTIGSVGILHKFLENSDPEIQLEGIRGISYIAARPEGIQSVIDSGSVKRVVEIMNHSETSTFVALTMIASMGMGQPSQSDYMIDLGGLKIILELLDNPSSRIKAHAFVGIGNFLANPSQIQKAIDLGFFSKFATFGNSATKDFNSLCEIVIIFNNATLHANEEQIEYFVSIGLIPLMCKLSGLEIKYASLDIPNLVLKTFINLFKTGDTLKEKNNGINPYTQAFKVANGLASLRFCVGSSRDPERSRIGSHIITAYFPEILNSNTPPLVNNNTATPSTTTTPPSTTSTTPSTTSTTSSTPPTANTTANSSADEQNSKGCIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWB2</accession>
    <name>A0A8J4PWB2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome P450 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_005476</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000220">
        <property type="protein sequence ID" value="KAF2073194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWB2"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20617">
        <property type="entry name" value="CYP1_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF417">
        <property type="entry name" value="CYTOCHROME P450 508B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="424"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2073194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="477" mass="55125" checksum="D4E9015BA564DC76" modified="2022-05-25" version="1">MFIILTFVFIFYIFYSFIYKNVVYRQKVAIGPMPLPIIGNLNLIKSPHIDLTKLGEKYGKVFPLWFGDRYSMIINDPVVFRDTIVKNSMSFISRPKNISFSLVSYGHINILNSEYNQWNRNRGLLKQYFTNNALIKTLSPTVNTQVSTLLDILKRKEKLNEPFKPRFYLDRINMNIILGFVIGKDLGFDLGDPNIMKLNKSLQDTIRLVSLGSPGDYINILTPFYSTYFKLFGSAIDEADNFMSSVYDERRAKYHPDNETHDIMDVLIHALPDPKERKCIVFMCLDLFIAGADANSNLGEWLFAYLSENPMIQEKAYRELVSVFGENGFATSEKKQSTPYFNAVLKEVMRLKPSLALSIPREVTEDITINDVFYPKGTIVIPNIYSINRSAEYWENPLQFDPDRFLDNEFNQAFIPFSLGERSCLGLNFANQTFYTIYANIILNFKFHPAEKVTYEDEASLVIHPKNDNSFILKSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWE6</accession>
    <name>A0A8J4PWE6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Brix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004309</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000150">
        <property type="protein sequence ID" value="KAF2074375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWE6"/>
    <dbReference type="OrthoDB" id="264354at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10480:FF:000002">
        <property type="entry name" value="Ribosome production factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10480">
        <property type="entry name" value="Probable brix-domain ribosomal biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044281">
        <property type="entry name" value="IMP4/RPF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22734:SF3">
        <property type="entry name" value="RIBOSOME PRODUCTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22734">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN IMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Brix" evidence="2">
        <location>
            <begin position="110"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="315" mass="36820" checksum="A7AE22853C457520" modified="2022-05-25" version="1">MKPKAPVDKDDISKDELKMRRSPNDIKCKSKRMELVQKLQAAKKTSREKARKQRKKERELLGEAAPPKQVPRTIESMRRADETMVDLNDAEYEDEIQKDELQSYFDGRPPKVCLTTNQKPSKNAIDFSKVFEKILPDAKFFHRRKYNIKEIVDFCNNRDYTDLVVINETEGVINEMIISHLPNGPTATFRITNITMPEDIPGGGEMTKHKAELIVNNFNTRLGFTVGRMFASLFPQDPNFKGRRVCTLHNQRDFIFFRQHRYMFESMEDANLQELGPRFTLKLKTLQKGSFDTKNGEYIHIHKQNMDVDRKKFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWK6</accession>
    <name>A0A8J4PWK6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">FUN14 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_003535</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FUN14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000112">
        <property type="protein sequence ID" value="KAF2075145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWK6"/>
    <dbReference type="OrthoDB" id="163794at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007014">
        <property type="entry name" value="FUN14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21346">
        <property type="entry name" value="FUN14 DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21346:SF10">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04930">
        <property type="entry name" value="FUN14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="180"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="50"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="54"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2075145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="255" mass="29040" checksum="37969C0C7C6DB052" modified="2022-05-25" version="1">MHKLVNPFLKNSFKSSFVNPFKGNTSRFSNNLYNVPNQIKSNQVLFYSTTTSFNNNNNNNSSNGNSHQSGNSSRSNNHNNGYQYQQQQQQYKFSTAASKSLKPFVGIGGVLAISATTALSSPVLCESTLKELDNRFATIPVEEYEEIKHLRKKSSFQRFLGITTLSGVLGYSSGFATRKIGMFVIFVVGSIFTLIQVLSYYNYLTIHWERIFREVNPKFDKENRSRYFRTFINILTNNLHVKAGFLSGFYLGFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX27</accession>
    <name>A0A8J4PX27_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_004402</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000155">
        <property type="protein sequence ID" value="KAF2074269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX27"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902554">
        <property type="term" value="C:serine/threonine protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047173">
        <property type="entry name" value="STRAD_A/B-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48014">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE FRAY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48014:SF28">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE FRAY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="59"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="343"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="434"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="348"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="464"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="481"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="502"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2074269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="523" mass="59033" checksum="CB48B08E57DBD4C4" modified="2022-05-25" version="1">MSSNISQFNTDGPVKDPSKRRSVPANLHSSISHERRESIIRELQKIPTSNQYPNTAEDYQLLEPIGEGTEGKVWKAHCISLNEDVAIKVVELERMDPAFVKDVIKEAKVMNGNNHPNLIHYHTSFLTDSSLWIVMDYLGGGSLADIIKYKYPNGLPEVLAITILKALLKGLEYLHSHQRIHRDLKSDNILIGDDGSIELADFGVTAIFEKNCLCSRKTIVGTPCWMAPEIISEKGYNQSVDMWSFGITAIELIKGKPPGSDLPPSKVFMNLLFGNSPSLAEEEERGECSHAYRDMVDKCLQKEPSRRPSASKLLEHKVFKYAKKSNYIVSNLLHNLSPCEDRYRESLSQEGSLNSSPNSSRPSSPDNYSANTDSPKSPIAKSCSSIELKNRQAGLPQFMPRAASHPVDLQNLEFQNNGTNSNSNQHIQHTQIVTPPSATEQKHQGFLHHKKSNPTSPEKERKKGFFNHFRRHSIAKLFGSPKDDHHKDEHHKEEQPTPHHGEQHHHFHFPWKHHHSPSHVEST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX68</accession>
    <name>A0A8J4PX68_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_002181</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000058">
        <property type="protein sequence ID" value="KAF2076503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX68"/>
    <dbReference type="OrthoDB" id="19711at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF713">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="5">
        <location>
            <begin position="187"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="316"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="356"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="396"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="436"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="579"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="621"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="110"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2076503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="654" mass="74614" checksum="88BEA845FF22F441" modified="2022-05-25" version="1">MLTDSFSFPSFFQWTKENGSMKNINNSSINKGTDDRVDKPQEKKLKSLNNSNSSNNSNNNMLSCQTACEVQIVPMAMDNDCRFLDYNIIDQSDLDLNNSFDDDDGMDYQTSSSTSTTTTTNTTTSISPFSLITYPLSLLKSIAKNNNNSSKKEEEDQQLIEQQLYQYQQYQQHQQQNQQVESIKKKINSFEELPLEIIVHIFSFLDYKDLNTRVSLVNKNIKEISEVYTIWKYLYYSEFRLAKGNKKKKLFHLDINSIYNNITPTAATSKLNNSNVVTESIGNNTTEIFKDYKQLYKKRFIVEKNWRRGICSVQTLYGHQDGVWGVQFHGDMLVSGCEDGVMKVWDINEGECLNTLIGHQDVVNSFHFEGDRIISGSDDSTLKMWNAHTGQCVNTFRGHQGSVWMLEFKDNCLVSGGDDKMVRVWDTNTGQLIQNLEGHSGRIYYVQMGNNLVVSGAQDRSCRVWDLRSSECVHSMTSNSPVHCLQINGDLWDGGDWAVASGHNNGSISIWNLRTGSLQAMLSNPLCCPVWHIQFRKNNIFTSSCNDLHSWNLGKALEPVNHSLDPTPTQHQLTSSKVFKGHTKSIKHFQVKDNRLVSGGLDNKIKVWDLDKPGNNYLYTLVGHTKSVDWLEFKKDKLISCSADHTIRIWDFYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXF4</accession>
    <name>A0A8J4PXF4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007413</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000394">
        <property type="protein sequence ID" value="KAF2071276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXF4"/>
    <dbReference type="OrthoDB" id="408373at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050228">
        <property type="entry name" value="Carboxylesterase_BioH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43194">
        <property type="entry name" value="HYDROLASE ALPHA/BETA FOLD FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43194:SF2">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN LPX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="99"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="363" mass="41338" checksum="658F38523D74D82D" modified="2022-05-25" version="1">MSSITIEKEDSNNHSSSTDYIENNEGKTNIDQEVPPILNPPFSDHSIEIYRDGHYAKLTNGWTWYQLFEPQVNHENSTITPRLLKNLKEKTFTMPTKFIVCFHGITWWSMSFHRLVQSFVENGYTVLLFDFYGRGRSCAPETLTYSLDMFVSQAIDLLDYLEITDMINLVGYSMGGAVATLFASTFPQRINRLIGMGPAIVPVPIPFIGKLVTMGYGVGKFLFSLFGAQSLLRKVETERFSMDIHEPENIDPLIIDDLVEKVKWLIEKKPGYLNSFHNTLCSIDFGRGNVELLTQITSQQNLKVLIILGNKDQIIPYEEALLIFKNNLPHAKIETIMNCGHAFTLEKPLELSEAIIKFLKEPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXZ0</accession>
    <name>A0A8J4PXZ0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">SP-RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_002769</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NSE2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000080">
        <property type="protein sequence ID" value="KAF2075921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXZ0"/>
    <dbReference type="OrthoDB" id="26899at2759"/>
    <dbReference type="UniPathway" id="UPA00886"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16651">
        <property type="entry name" value="SPL-RING_NSE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1010">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026846">
        <property type="entry name" value="Nse2(Mms21)"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21330">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE NSE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21330:SF1">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE NSE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11789">
        <property type="entry name" value="zf-Nse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SP-RING-type" evidence="11">
        <location>
            <begin position="152"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2075921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="250" mass="28546" checksum="08C45A32A40C52ED" modified="2022-05-25" version="1">MSNLQMDSLPQVQDLDRMQKNYAKIQQLIDSHMFNIHQAALSLATENEQSELFKDLDKSYLTCLGLKDFLSKHDGDINKLKNEVAKATQSSQSSQFSQRIGTPDLAADFFKHENMLKNLNTTPYEKTQKFKDYRLSIWNANHSEPMDNPGDEDEDLVIASQTFDINCPISKKILEDPVRSKDCNHVFSKVHIYALLSQNRGGMLCPVAGCSKRVDASKLELSAEHVEMVKRELRRRKAEKPKEVQDITDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYR1</accession>
    <name>A0A8J4PYR1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial</fullName>
            <ecNumber evidence="4">2.1.1.201</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Ubiquinone biosynthesis methyltransferase COQ5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">coq5</name>
        <name evidence="5" type="ORF">CYY_006893</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Methyltransferase required for the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 2-methoxy-6-(all-trans-polyprenyl)benzene-1,4-diol + S-adenosyl-L-methionine = a 5-methoxy-2-methyl-3-(all-trans-polyprenyl)benzene-1,4-diol + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28286"/>
            <dbReference type="Rhea" id="RHEA-COMP:10858"/>
            <dbReference type="Rhea" id="RHEA-COMP:10859"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:84166"/>
            <dbReference type="ChEBI" id="CHEBI:84167"/>
            <dbReference type="EC" id="2.1.1.201"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of a multi-subunit COQ enzyme complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion inner membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. MenG/UbiE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.201" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000338">
        <property type="protein sequence ID" value="KAF2071786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYR1"/>
    <dbReference type="OrthoDB" id="6329284at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008425">
        <property type="term" value="F:2-methoxy-6-polyprenyl-1,4-benzoquinol methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000208">
        <property type="entry name" value="2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01813">
        <property type="entry name" value="MenG_UbiE_methyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004033">
        <property type="entry name" value="UbiE/COQ5_MeTrFase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023576">
        <property type="entry name" value="UbiE/COQ5_MeTrFase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01934">
        <property type="entry name" value="MenG_MenH_UbiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01209">
        <property type="entry name" value="Ubie_methyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51608">
        <property type="entry name" value="SAM_MT_UBIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01183">
        <property type="entry name" value="UBIE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01184">
        <property type="entry name" value="UBIE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="174"/>
            <end position="175"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2071786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="302" mass="34210" checksum="FCDC833789268DA4" modified="2022-05-25" version="1">MIPSLRRLTTGTYGFVSRRFFTTATPKDNNTTNEQPQTHFGFQTVNKNDKESMVKEVFDNVSSSYDLMNDVMSMGIHRLWKDELISTLNPTPGSHLLDVAGGTGDISFRFLEKIKQSPYYLPAIGANPPKNTSLPSSATVFDINQSMLDQGKKRGILNGYNETTDPSIDWVQGNSENLPFKDETFNCYTVSFGIRNCTNVDQVLKEAHRVLKKGGRFLCLEFSQIPNPALRYMYDTYSFNVIPVMGQLVVGDRDSYQYLVESIRKFPDQETFADMMRDAGFQQVTYKNLSFGICAIHSGWKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZH5</accession>
    <name>A0A8J4PZH5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Oligopeptide transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002845</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000083">
        <property type="protein sequence ID" value="KAF2075859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZH5"/>
    <dbReference type="OrthoDB" id="627262at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045035">
        <property type="entry name" value="YSL-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645:SF0">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="278"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="311"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="335"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="372"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="427"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="452"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="538"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="590"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="663"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="693" mass="76655" checksum="683042C1F27E8FE8" modified="2022-05-25" version="1">MMNRLHEEEEEENGVILVDSDINHETYNSSDDDYNESLRNITDKKGNEHDGTDIHLKQITFRSIVAGIVVGGLMCFSNMYFGLQSGWVTMGSLQSTLLGFLFFKLLEKRLAIKFNQFENVLLQTIAVATATMPLAGGFVGIIPALKVLYQGDNPDTQTLPSYLSWWALTLWAFSLAFFGVFFAVPLRKQTILVEKLKFPSGLATAQMIKVLHKLRNDQVHPELLMQEEEHHQHHPSAIDQDNSSAEFETKELTNSSQIMMQEDEYTEQEKYWNTKWKVLFYSFTASALYTLFSYFFPILKLLPVFGTNAKMFFWGLTPSLSYVGQGMIMGTKTGISMFVGAVLGWAILGPMAKALGWAPGAVGDFTTGASGWILWISLFIMLSESIVSLLVLLVQYVLHRFGIGRASVYDDKSSADPAPLSERVPHMWWIIGLVVSAITCIVIVSPLYDVKFYETIVAVVMALLTSVLAVRALGETDLNPVSGIGKISQIIFAVIAPKNILSNLVAGAIAEAGAQQAGDMMQDLKTGHLLKASPKVQFYGQLIGSFFSIFFAVLSFELYSYAYDIPSVELRAPTHVVWLSMAKLVNGGQLATNVLGFCIVAGVLVSFIPIIEAIKPSTERFLPSGIALAIGMYVQPNYTIARCIGTVAQFYWKRKNLNSFREYMIIVASGFVLGEGITSIITAIMKAIGVPHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZS2</accession>
    <name>A0A8J4PZS2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000433</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HesB/IscA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000008">
        <property type="protein sequence ID" value="KAF2078243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZS2"/>
    <dbReference type="OrthoDB" id="333486at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.300.12">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016092">
        <property type="entry name" value="ATAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000361">
        <property type="entry name" value="ATAP_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050322">
        <property type="entry name" value="Fe-S_cluster_asmbl/transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017870">
        <property type="entry name" value="FeS_cluster_insertion_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00049">
        <property type="entry name" value="iron-sulfur cluster assembly accessory protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10072:SF41">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY 1 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10072">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01521">
        <property type="entry name" value="Fe-S_biosyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01152">
        <property type="entry name" value="HESB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core" evidence="2">
        <location>
            <begin position="19"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="135" mass="14921" checksum="B26B00F71EEEBED6" modified="2022-05-25" version="1">MSISGVVKLGKPSRKAIFSLTDSASKRIREIMKENKIENCIGLRLGVKERGCSGMSYTLDFATQKNKLDEVVTDKDSKALLSVIGTEMDYIEEPIKKEFIFINPNATNTCGCGESFTTKDYSTPFSLPPKNNVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZS5</accession>
    <name>A0A8J4PZS5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_002101</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probable methyltransferase.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000055">
        <property type="protein sequence ID" value="KAF2076615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZS5"/>
    <dbReference type="OrthoDB" id="20143at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050869">
        <property type="entry name" value="H3K4_H4K5_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197:SF44">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="7">
        <location>
            <begin position="38"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="MYND-type" evidence="8">
        <location>
            <begin position="86"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2076615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="435" mass="49766" checksum="C2159B70F36465AF" modified="2022-05-25" version="1">MINEQKNAVDIHLRNHKSNSNKSNNNNNNNDNIIEENDLIIVKYSADKGKHVIAKTQIPKGDYVSFREKPFVATVNFKDPFEKEICHRCFIYFDNKDSHDSCSCQQCKSVFYCSEQCKEHDQYSHNLQCGFLKKLLSASLFDHEIKTLMKMIVKILVQNHIDDISDSNNNNNSSSPLNSTTLLLKDIFKLVSNKDRFTSQRIGDFVKAFRFIEKSCFGGLQQDHPLLKSFGVVDSKSLEFQNKMIELMCILECNAHEIGITIPSNTNTCSYRYCSIGAGLYYTSSLFNHSCQPNISKIIEPGQSYGNHTMLAFKDIEMGEEICFNYIPISLAFDDRQTKLLYSYFFKCACPACLHVPKKTNNNKKNNNNNNNNNQFHNRFLKQYLCKKETCNGIITPFKVSPSSVNNTLTSLANDTHEQDIEYSCNICNPHILGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZV0</accession>
    <name>A0A8J4PZV0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_000380</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000007">
        <property type="protein sequence ID" value="KAF2078288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZV0"/>
    <dbReference type="OrthoDB" id="26095at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000001">
        <property type="entry name" value="Tenascin C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057015">
        <property type="entry name" value="B-sand_ComC_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053331">
        <property type="entry name" value="EGF-like_comC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013111">
        <property type="entry name" value="EGF_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24032:SF76">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24032">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24143">
        <property type="entry name" value="Beta-sand_ComC_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07974">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="905"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="619"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="623"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="642"/>
            <end position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2078288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="952" mass="105899" checksum="60BAA05092D2DFBC" modified="2022-05-25" version="1">MAVRAGLSNQYPINPTYGRRDFCADDYNFTCSSDGNLGWGTLTSITKNVFDKSYFTCFKTNPIAFYCSNCNGITSISQPVASLGILWLETGPDTVSNLEGTVKLSYLKNTYDIRFDTLLNYDKMNLYFENDLQPSDNFSPFSFHVTASNIVNLTYATVSALEYTLDTNFDQDWSNIHTWTQVNGFWVWGSIATASTFPIEFSNMPIKSLLYTMSFSYIAFSVTSKVSISDNYDRLNEIYFYPGFNFNGDIPFSHIPPSVTKFTMTNGNYENITVDLFINHPNINTLNLANNNINGTLDNNICRVGTFALQSNQIGGELPSCITCHLSFKSLNNTLARNKFTNINNPPPCTTLTAYMRVDVATNRTYLYGTDLGFSNVYFKSTPTKDWKVDIPNQSFYIVGAIPQIYNLTFLYSNHTFLFSTLMPPNVTLISVDSKTITFNGTCFSNNKTEVSVVVGDVDCVVEYSDFNSIICSIPSSQWKNVTDMPTTINIGGMNTTVNVTMVRPEPRCNCQDRGECDLVTEQCKCYFDYQGPECYPQHYVTSVKESTVNGGDVFIYGNFSYYHNNPNVLIGNSECIIDELNSTTILCKSQPGTGIWDLVVNQNNLTWVGSNVYQFKPLTQQCPQNCNQHGECDQITGICICNDGWTGLDCNAGSFVNSETPGSRPPSVITIGVDGTGTINNEQTKFDILITALIEYTNKGEVVQVYDLKSKWSLNSTSNNTNVFKQKLENTNCVLTTTMEEIKQDKVLTFINQQVALLAGSIKISVDVQGYPYASSLNYIQLRIESIAGTLDNSQEPNPCNNDTTTIQTNSPVNNDQIQLDYMVMKKNSKVMYGRFINRVLSDGRESLLTTTVISKDSNSIVVGLNLPHCKSCLIDPDFSVLLDSDFKSKSQCANVATPNKKGYVIPVAVVVSVAGASIIGASAFLIYRKKFVEGQLQMKLKKLAQFNSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZY1</accession>
    <name>A0A8J4PZY1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001583</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000038">
        <property type="protein sequence ID" value="KAF2077136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZY1"/>
    <dbReference type="OrthoDB" id="15871at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="3">
        <location>
            <begin position="54"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="191" mass="21328" checksum="84E027D94734F46D" modified="2022-05-25" version="1">MELLNEDIIKEYYVGNENVFTLGKQNYMNGSIQLEFVGKYLVKSKISESPSHRAMIELNVNGGLKKTLCTCKEYQFILPCQHIVSTAFELIDYIKDHSNNSLTNASSAPASSIPSLQRTVGYQILLKEYSFSKSNEVRDLLVEMNNTIAIETQVEEAVNNNNSSNLVEKDENNNLTPSAPAVNQSDLVVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZZ7</accession>
    <name>A0A8J4PZZ7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carbohydrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001351</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000031">
        <property type="protein sequence ID" value="KAF2077348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZZ7"/>
    <dbReference type="OrthoDB" id="19156at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019028">
        <property type="entry name" value="CBM_49"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052879">
        <property type="entry name" value="Dd_Spore_Germination_Stalk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33239">
        <property type="entry name" value="CELLULOSE-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09478">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01063">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035174304" description="Carbohydrate binding domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate binding" evidence="2">
        <location>
            <begin position="62"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="165" mass="18220" checksum="26D2988CF64F5878" modified="2022-05-25" version="1">MKFLSLFSALLLVLAVVSAETDYHRPRPPYQCGPLACENGQVCKIVNGQCQCIPSQQLVQDVTLTQKVIGTWTVGSTHYTQYDVVIQNNMNRNIKNIIIDTDSTFKLRQPASANLWNMVMLANGDLVLPSYQPSINAHASYTFGYILVGTPATPANLAIKAIIYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q004</accession>
    <name>A0A8J4Q004_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TRUD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_001502</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000036">
        <property type="protein sequence ID" value="KAF2077181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q004"/>
    <dbReference type="OrthoDB" id="447290at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02576">
        <property type="entry name" value="PseudoU_synth_ScPUS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.20:FF:000003">
        <property type="entry name" value="Pseudouridylate synthase 7 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.20">
        <property type="entry name" value="TruD, catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001656">
        <property type="entry name" value="PsdUridine_synth_TruD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020119">
        <property type="entry name" value="PsdUridine_synth_TruD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011760">
        <property type="entry name" value="PsdUridine_synth_TruD_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056961">
        <property type="entry name" value="R3H_PUS7L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042214">
        <property type="entry name" value="TruD_catalytic"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00094">
        <property type="entry name" value="tRNA_TruD_broad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326:SF21">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE PUS7L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25094">
        <property type="entry name" value="R3H_PUS7L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01142">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037016">
        <property type="entry name" value="Pseudouridin_synth_euk_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50984">
        <property type="entry name" value="TRUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01268">
        <property type="entry name" value="UPF0024"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TRUD" evidence="5">
        <location>
            <begin position="322"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="460"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="668"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="677"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2077181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="689" mass="78156" checksum="06BC8DB28D4718F7" modified="2022-05-25" version="1">MSDKIENQCVDQTTATVETTTTSDEKTSVASSLSTNPPTTTNTPQPTLAKLERTEETFGIKHYLSKTKPFTGLLKHRFTDFIVNEIDENGNVVKLVDTNYQKDEKTVEYDTTPEQDLEKLVGEEKTKSFMEFFNGPNKTDAKSFFIFDKDTDKDHRTLVHKVIKTRFILATETTNDNEVKVWPEANPKKKAINDRSWPADKPKYVQFVLAKENRDTMDTISLLSKYLRNSPKQFSIAGTKDKRGVTSQRVTVYKITPDRLYDVNVKLSKLNPPIRIGEYKFVQKQLMLGDLSGNRFSIVIRNVQGANDQEITESIEQFKQTGFINYFGLQRFGTGSIPTHEVGRAMIQGDWDKAAALLLDPREGERSDALAARQCYKETGNIKKTLDMMPKQLNSERMLLMGLKTSKSSQDAFFNIPRTMRMLYTHAYQSYIWNHMASKRIELYGLKAVVGDLVIVSEEEKNPKPSAATESDDKEKADAEPVEQDIEQDETSLSKKVIKVGYVTEQDVLDNKYSIFQVVLPIIGSTVDLPKNEIAEAYMKELEKDNIQLSQFVTKNRAIDLRGDYRRLLEKASDVTFKILNYDDATLPLAVSDIDALKKLAEPQDIPTGQHKALRVCFNLSSSTYATMAYREILKVPSDIISQMNMANSPSITNSVLNKRKLDELESTNTTTTTIGDNEKNDNKEIKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q083</accession>
    <name>A0A8J4Q083_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF3472 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000003">
        <property type="protein sequence ID" value="KAF2078513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q083"/>
    <dbReference type="OrthoDB" id="5576763at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021862">
        <property type="entry name" value="DUF3472"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11958">
        <property type="entry name" value="DUF3472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035316308" description="DUF3472 domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="29290" checksum="CE79211904FAB6EB" modified="2022-05-25" version="1">MKIVCLIIGFILATVVFSDPNIDFNWGNHAGNIGYDKAVLPIKVLVAPTIGKTYYYALCNKFLGDDQSAIYMGLQPRQPGKNLVIFSTFGTGTQSEHSQCVSGADGGSGTSCSLQYNWNLNELYYVVMQRASMNNTHQSWEGYIVTEQGQATLIGRFSVPSTRRGLKPSSYFFDEYFPFNGASKDPTKRKCIEYTKFSITAPLASYNNQWYPSTPTNIRLDTGKDSCAMYWNKNNARANETFSNGFYSYDIENGFLPPVPTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0D8</accession>
    <name>A0A8J4Q0D8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000024">
        <property type="protein sequence ID" value="KAF2077603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0D8"/>
    <dbReference type="OrthoDB" id="24318at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="545" mass="61461" checksum="AAD08745842E881D" modified="2022-05-25" version="1">MNELFNSIWRDQFLRSKIRNQRFNHRTIGVSLSYLERNSKYLALLDTKTHDIYIQFSTETELSKYLAHPLNAMFNSVRLQLKSTECLEGVKFPSSVRQFSITFLSGQLDLAMLSLCTNITDLTMCFRNVSTTYRGDLPAGLKRLDIKGVESEQHKPEIGPLPESLTSLTVSNYKLCLPESLPPSLTLLKLSWEIIEDASQKIIVPASLTDLNVQEEEMYIIKNRFTVPKGKLFSSARVKLLTEEDVEQLNQCPWVTNVTIYNNSLLKLLPSTIKSIYFFGGQDKDAGFTAGSLPPLLHRLSANQVDYPIVQGFLPSTENTLTILELPQYNHVIPSGLLPASLTKLDISSYNHPITQVGVIPSNIKYLSIKDVAKISANVLPSSIIELNIYPDKLMISNNVLPHSLLTFRAYFKRISSKILVFGQSVTLPKSIINLSLFSDSDSKSQSIKSNLIPPNVRNLELSNIIIKKGMIPPSCFYLSTNYHPIKEGLIPKNVKQLFITHYEKIDFIPPTVELLKLNNPLESYPFSDLTVKRNGVLYSSNKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0N6</accession>
    <name>A0A8J4Q0N6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002818</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000082">
        <property type="protein sequence ID" value="KAF2075882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0N6"/>
    <dbReference type="OrthoDB" id="21595at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00014">
        <property type="entry name" value="CH_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050606">
        <property type="entry name" value="Calponin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385">
        <property type="entry name" value="CALPONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385:SF14">
        <property type="entry name" value="TRANSGELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="2">
        <location>
            <begin position="10"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="291" mass="33194" checksum="DCA3B80D53707CBC" modified="2022-05-25" version="1">MNSSNNNNIQQKEQAIKLWIEYILSTKLDTSKSLYELLVDGRILCKIMNKFHNRIDYIHYSDVDPVPLFKRFENITLFLKKCRLYLPQEYLFHTNDLLQNENIEAVYDCLLHLSHYFELNNSNNNDIQNTSFNELFDFEDSGSNSSTNNDNNVVINNSNNNTKTTTKDTIKNIQVGNNIKPNTNTTNTTNVNNIIKKKGINGYILDCLVEVKYTIVIPFLVFMTMGFGIKMGRSMYISLSQFNYSNFLANLSTKTTSSGNSISSIIGNSSSGSSYFSNFISKLSLSDNNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0P2</accession>
    <name>A0A8J4Q0P2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000904</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000019">
        <property type="protein sequence ID" value="KAF2077783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0P2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035150761" description="Chitin-binding type-2 domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="87" mass="9981" checksum="7A60E695D7FCCB81" modified="2022-05-25" version="1">MKLLYIFLIVALFVACIAAESNRRIPCDGYCDCDEVNKGCPKPRLCKRPHPRDQTLYIQCSYHGGAYTYRCREGQVYNDQALRCVNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q164</accession>
    <name>A0A8J4Q164_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_000779</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000016">
        <property type="protein sequence ID" value="KAF2077901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q164"/>
    <dbReference type="OrthoDB" id="410592at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF5">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1168"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="2">
        <location>
            <begin position="8"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="2">
        <location>
            <begin position="441"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="2">
        <location>
            <begin position="531"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="2">
        <location>
            <begin position="707"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="2">
        <location>
            <begin position="794"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="3">
        <location>
            <begin position="940"/>
            <end position="1152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1216" mass="131058" checksum="26A255EA6020C2DC" modified="2022-05-25" version="1">MLCVGGAPIISRIYPTWGLIGNDINIEGTGFSATVALNSVSASGYPCVIQSATTELIVCHLEDESIPRANALDISVSTSSVVSNSKPFGLIEIGGYGQNNKFYNWTGGVYSSIPDKSKIAVRSVDLSVNIPVTYEDHQNMYFILNVEMAKQSEFQLIDSSTNSVVSLYKPLLGFYVIVDDVKFYENSMVITGFAYTSTTTVTTSQIMGQNDPCTITSFSDTSITCTPVSTYFINTMTYPFTIIKSGILKVFTRIPTFIQGYENLGVDICNLNVSSTNLGQTIRIIGLNPTPIVPTTTTISTTDTIVSFTYPLNAQCGYAFVSTDDSTSFQRLTNSMIICPTPTVQTVIAKPDSSNNNIILFNGIFLNEQLYGNTITTTISYTIQFGGGAIMSCSNPSHLWIPANSTYTASCQVSATPTTSFMLIAATLDGQSSSILVGYQPTITGVSKTDYKVPGVITITGSAFSSLDLTVLIGGSQCVNPVVQGDGLQMTCTFGSDVTVVDFSKPLEVFVSIGSTYNDKKSLFYYNRPTPTISSSTRTYFGVPGTVTITGTYLYSTNLVVTIGGSPCTSPLSAPDGNSIACQFDSSVILTIPLEVSVTIDSTYTAKNSVFYYELTTPTLVGSTSTVYGTSGLVTITGTSFYNFGLVVTIGGATCSTPIASEDNKEITCQFAGVTVTDIKNPLEVSVTIATNYNAKKSVFYYTLATPTITSSTKSLFGTPAIITITGNSFFNFDLVVLVGGSICNNAIASQDNKQITCTFKSDVAAGVNTPLEVHVSMNTNIEAKANVFYYAKPTQVSISSSTSTKYGVPGIVTIFGNNFINNQLLVKIGGSECTNALATQDSKQLTCRFKSDVVAAENTVLDVFVSVQSLFNATEPVFLYIKSDQNCPIGSNGQECSGHGTCEQQYLCKCNKDWESSDCSIPNNGGGTVIPDPNVNPNDTSSTIITPGGTMFDVGIVLIQEIDSDHNIVQSYNVTSIKWTNITKQDNQHIYTTTLPNNQSTLNVKLSINNLDENVYYNFAGDVIPILPKSIKYQVELQNYTFSSSLNTMQFIFKSGILQEGGECVYESDTNTQPTSGDSIRSILMMLNGETLIGTFSNRIVLDNRPSYNQVNKLTDTQINQYNLNSQSLYVAITTTSFKNDVVVDPNFGVLVTSTPDNQNQCNKKFAAWKIAMIVVCCVVGVALLIATVMLIKKRAVVKAFNAKLKKLDNHQANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q238</accession>
    <name>A0A8J4Q238_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tyrosine-protein kinase ephrin type A/B receptor-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_001383</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000032">
        <property type="protein sequence ID" value="KAF2077317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q238"/>
    <dbReference type="OrthoDB" id="20798at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011641">
        <property type="entry name" value="Tyr-kin_ephrin_A/B_rcpt-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46104:SF1">
        <property type="entry name" value="GENE 9195-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46104">
        <property type="entry name" value="GENE 9195-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07699">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035295901" description="Tyrosine-protein kinase ephrin type A/B receptor-like domain-containing protein" evidence="3">
        <location>
            <begin position="29"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="461"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein kinase ephrin type A/B receptor-like" evidence="4">
        <location>
            <begin position="360"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="113"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2077317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="513" mass="54175" checksum="118B4BF888AB963D" modified="2022-05-25" version="1">MRNSISLLLKIFLLSFVLISLYNGLANSERVKIAITDTDKLVENDNSFLERKHRNKGDFPPHDADDGDQGFTPIDENSDGSDSNQDSKSESSNSNSTEPTEAPTFSPDPTPTPSNSSSESSHSSEEPTNTPKPTPSNSSSESSHSSEEPTVAPTVSPDPTPTPSNSSSESSESSKSESESSHSSEEPTNTPKPTNTPDPTPTPSNSSSESSHSSEEPTTTPKPTTTPEPTNTPSPSPTPTPTPTPTTTPKPTPDNSTCQMCPAGTFANIKNPNCSGCNSGSCKSPTANCELCPSGQYSNVQGASECQPCLDGTTAPLNGSATCSSCEAGYSSDSEHIKCVPCAPGLFSNEGEVCQDCAPGQYSNKGSPFCSKCPTGTYNGFAAQQSCFDCGTRFYNPEQGSTSSNKCLLCPSGSYCPFTATENAVVCPEDHFCAEGSMSPTKCTSFYTSSLGSDDCHPNKWLYVIIFGVTGAIVVGIIVVWKYVQIQKYKKAQYTSEVTRLIPKPEGPEYGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q239</accession>
    <name>A0A8J4Q239_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP adenylyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001386</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000032">
        <property type="protein sequence ID" value="KAF2077320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q239"/>
    <dbReference type="OrthoDB" id="17043at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003877">
        <property type="term" value="F:ATP:ADP adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008796">
        <property type="term" value="F:bis(5'-nucleosyl)-tetraphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004780">
        <property type="term" value="F:sulfate adenylyltransferase (ADP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009164">
        <property type="term" value="P:nucleoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009165">
        <property type="term" value="P:nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009163">
        <property type="entry name" value="Ap4A_phos1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043171">
        <property type="entry name" value="Ap4A_phos1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045759">
        <property type="entry name" value="Ap4A_phos1/2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019200">
        <property type="entry name" value="ATP_adenylylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053364">
        <property type="entry name" value="Fork-head_TF_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42746">
        <property type="entry name" value="DIADENOSINE 5',5'''-P1,P4-TETRAPHOSPHATE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42746:SF2">
        <property type="entry name" value="DIADENOSINE 5',5'''-P1,P4-TETRAPHOSPHATE PHOSPHORYLASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19327">
        <property type="entry name" value="Ap4A_phos_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09830">
        <property type="entry name" value="ATP_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000846">
        <property type="entry name" value="ATP_adenylyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ap4A phosphorylase 1/2 N-terminal" evidence="3">
        <location>
            <begin position="3"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="ATP adenylyltransferase C-terminal" evidence="2">
        <location>
            <begin position="255"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR000846-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="355" mass="41000" checksum="6BC2BBE06F38F01D" modified="2022-05-25" version="1">MSSMALWDKIKKVSERATQIGAIEKIESSPLFINENGIKFMVSVAKSLLKRPFNFQKSISTENISSLSNSNNINNSPKEILQPPKKFIDPFLPCDKDLFVQELCGTHNLVLNKFNVSNYHSIIATKEYEKQTDPLNRYDFKAIWECIIDCNMLCFFNCGPSSGASQPHKHVQLLVTPFHTCEEKDLTCKLEQLQQQQLSDIDNDYLKCPIESIIKLYNEPKETIFQVEQLPFKNACIYYQTDKLKSMDKDQISIYFEHHYRSLLNHLNLNSDQDNSSSNNINNIYEDTTTDFKSYNFIMTKEWMFMIPRSCYQAKGISINSVGFTGAILVRKEEELDTIKSYGIMNILKDVSFSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q268</accession>
    <name>A0A8J4Q268_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000793</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000016">
        <property type="protein sequence ID" value="KAF2077915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q268"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35885:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35885">
        <property type="entry name" value="CARBOHYDRATE BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035311949" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="316" mass="34647" checksum="A67836A2366AAD23" modified="2022-05-25" version="1">MNKTLFCLVVFLCVATFTSSADATNYGVVISNSNDTTIHIGLVEYLNASYAPPPSLVITGLVYSGGYYQQSTYNYNTQVLTFIATDPVTQNTFLYNVDCNRWRIINSLSLPGGAGFRGLASDQTNNNDPYNIWITSQTGNGYVIVSKIDPLTLKAVVYDTIVGTYYTSTLGSNQNYVVTYGNSSGIFVNVYSTLYRKISQQKFSFANIAVAPKGPINMIYSQLTNTLLATVIVSTGEASSFSTLAFVDWKSSTFQITTMASFINSIPSVNVPDVNNFNYIYSVGYVGSQYYLYSYNIYDQQLLNAAPISIPIAAAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q411</accession>
    <name>A0A8J4Q411_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BolA family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_000862</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the BolA/IbaG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000018">
        <property type="protein sequence ID" value="KAF2077817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q411"/>
    <dbReference type="OrthoDB" id="4983at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.90">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050961">
        <property type="entry name" value="BolA/IbaG_stress_morph_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46229">
        <property type="entry name" value="BOLA TRANSCRIPTION REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46229:SF3">
        <property type="entry name" value="BOLA-LIKE PROTEIN DDB_G0274169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="97"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="124" mass="14049" checksum="66B2E2E03EF2C5B8" modified="2022-05-25" version="1">MSAADRPVENEIRQKLIKEYNPIHVEVINESHMHNVPKNSESHFKVLVVSDVFTPLSLIEQHKHINNTLADYIGTGKIHALSIVSRTPVQWDRIQKKKELEQQQQQSNSSLVDPSPSCKGGFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q468</accession>
    <name>A0A8J4Q468_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mechanosensitive ion channel MscS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_000836</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000017">
        <property type="protein sequence ID" value="KAF2077874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q468"/>
    <dbReference type="OrthoDB" id="544685at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016688">
        <property type="entry name" value="MscS-like_plants/fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31618:SF1">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31618">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="225"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="268"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="344"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="525"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="554"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="5">
        <location>
            <begin position="571"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="184"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="48"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="137"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2077874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="762" mass="87114" checksum="62C88B46DFFD322D" modified="2022-05-25" version="1">MKSPKTDRDYQLVDEEQQKTRRRSSNNSNSSNNNNHRVSLSLPPPTASINNSDNISINMDPNNNNNNKSSSKIKSPLSDTGERKLTRINSSGEDTIVLSPKDEKDGTHKIVNRANNLTQSDPSLNITCTDIKPPSPVNNGATTAVNSNNGSHQGGLHTATSFPEIQKHPLGKAQELYHVVSVGDDDDDHHHHHHDGGDPPPSDDEDDGDEEKKVKKKKRGFFRQLFTTKFMIIIILLLLVLVSAVGVVFRIFWEDVEIVDVVVLRWALYIDICVFSYMAVYFIIRGFISLFESTLYLQQHVYYYINGLVRPLSCLIWAIIVYFATDPVLQLPDYVDDDMDKFFIFLRAVMYVSLFYCGRVALVKVLAARTNRKAFYTSLKRSLLNEELLDQLSTKNSKNLTQTVSTSIKKKKRMGITLWIESLKKRNQLSGKLSSIAENYTQKDAKIVAKRILANADRARKGYLVKDDLKYYVKAKHLDKAFDTIGSLHDNIIKRDDLINWILRVVRSRKTLEYRLRDHEDIGRVINEIVNFIFWILMFLFVMTLYGYEASVFLVPLSTTILGLSFAFGTTLRNVFESLVLIFFVRPFEAGDKVVLGNLEPLIVDKIGILFTSFISWDGKAVYMPNPVITAARIENHQRSEEVSVSIDVTVNFNTPIEKLYGLEAKFDKWVKAQPEKWRPDVWMSFASVLGTNQITLKYGGSIIASWQDSKRWRPCKNEFYIKMKDWISEANLETYPARQQIELLHPIPIELSHFQPPVRPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4A6</accession>
    <name>A0A8J4Q4A6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome-b5 reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_000142</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the cytochrome b5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000003">
        <property type="protein sequence ID" value="KAF2078517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4A6"/>
    <dbReference type="OrthoDB" id="260519at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06183">
        <property type="entry name" value="cyt_b5_reduct_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001834">
        <property type="entry name" value="CBR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370:SF207">
        <property type="entry name" value="CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00406">
        <property type="entry name" value="CYTB5RDTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0274">FAD</keyword>
    <keyword evidence="3 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2 10" id="KW-0349">Heme</keyword>
    <keyword evidence="7 10" id="KW-0408">Iron</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="12">
        <location>
            <begin position="28"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="13">
        <location>
            <begin position="177"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="140"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601834-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2078517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="445" mass="50563" checksum="35F2CCBBE1DCB7AF" modified="2022-05-25" version="1">MTFGTPMISDMIIKLAEKTTERHRGKIGQFYTFEEISHHNQRHDFWTIINGKVYDLTDYLMMHPGGYNLLFKQGGRDGTHDFEGMFHSRNAKAILEKFYIGKVLPSQTHVLPHPSHSNDNNSLSPFKVPTSIHPKSHIKSTTTTTPTTSTTTTATTNLSTDINMSTSTSSLQPPLEIELIQAKIISKTKLTPDTYNYQLEIDDKYKHLNWISPLTHVSLSSVDLYKDIGFKSYTPIRQSNNVLEFLIKGYENGTISKLIHNSKPGDYLMLKGPIQTSQSFEIQVSSSPQSPSFLLLIAGGTGITPMLQVLYDKLNQQKERVCNIVLIYSNKTREDIIWREQLDQLKQQFGTHLYIHYVLTANQSPIVEPMMDTTTTVESFNRLSKYHYGRINEDMILSCLEPILAQGIIFTLSDALVCGPVEFNRFISDQLFSIGYSLNKIHLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4J7</accession>
    <name>A0A8J4Q4J7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004869</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000181">
        <property type="protein sequence ID" value="KAF2073811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4J7"/>
    <dbReference type="OrthoDB" id="19024at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="373" mass="43409" checksum="8AD10F920E4747C8" modified="2022-05-25" version="1">MNINNVAVNQSKFLLRAFDQIKDMKPISEISRLSTSTKLDAMEAISHPKDFTLRAGMTQRNKIIVAEDIESKRSVGSYGCNIKDMKVFGRKKTVFYAFDFTVNEEFRNKSLGKLFINHLNQSVNNEYLKNPDNEPLLFASTSKSNGSINKVLGGVEMKEFYDQRQCAWKLDEEMVLPSMHGSQFSVHVYQEKRLKQVKRQWEDNFKSFDMTPYDFNDILVHNNQYYETTYVAQLKKDDKVFGEASISLWNQDKIFSLKDKKGSSKQHRQLFSCYSNGFEKFFTFDYLLKYVHNDQMRKGVDYLFGGFAINDPIIKHFPLVNGIKNLDFTANVRLNNQQESKLLQKSRDESTPYFNDPRDYGVVAFHELGKTMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q6B3</accession>
    <name>A0A8J4Q6B3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003857</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000127">
        <property type="protein sequence ID" value="KAF2074831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q6B3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="167" mass="19952" checksum="167534102FFA360F" modified="2022-05-25" version="1">MDEKKDTMACDRHGSPKGYTHLQQRDARFLTIILYQDHDRAIKLFYFNMKSLNFGFNFLYFLDDQILSPVDWDSKHLYDKVKLFLSSTNEICFKYIVSSNVPNLQYYKDKRIHYKPFSIANISTNNDWTNICIFNNADKGFTLNSYDNLYHCIACKTNIKYWNNHHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4QAN3</accession>
    <name>A0A8J4QAN3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000622</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000012">
        <property type="protein sequence ID" value="KAF2078071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4QAN3"/>
    <dbReference type="OrthoDB" id="20527at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035316316" evidence="2">
        <location>
            <begin position="20"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="363"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="404" mass="45406" checksum="1D134AE25D4700AE" modified="2022-05-25" version="1">MNIIIVIFLLSTCCNVISSTMFGGYMSIEEDREKISSTYLFPTNSNDFIIISTLHGDLFRLKVSQQRIVKNVVVDNLYQNQESGSTSTGYLDDDNNRFYFATTRNSFNTTPPTVDITCFDGQQLSVLNSFMIENESIPFNQNSFFVDVTTGYIYLIGSSRIFKFVYNVQGLQFVQSITYPISCIFPSSAAFNSNKQMIYLGCTASNGFLYEISMSNLNVTHTHMYYYGESILSLLLAPLDSYTFIGGTPKGRGITDSVPFHIVSFSIDGQDPGEYRDRQVSYGEPSTAASDGKQYGVFVFEHEIMLINLVEMDNSVVGLDYYESFVGLTSVYSSSTNQVIVTTNSSYIYFYNMPNTTPPHLVVPYYIAFPVLIVGILIILLICKFIHSCIKCKKSKNSDYTPIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UNQ6</accession>
    <name>A0A8J4UNQ6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010408</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001092">
        <property type="protein sequence ID" value="KAF2068266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UNQ6"/>
    <dbReference type="OrthoDB" id="2378640at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="57"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="260" mass="30637" checksum="FF470232BB3B06CA" modified="2022-05-25" version="1">MDTYYFPKVKCLFCKQEQTLFKLEEHVYICLNDILKQNDLRPFVTSTEINKLEDKHKDQIKILEDKHQDQIKILENKHQNYINLIRDKHQDQIKILEDKHQNDIKILEEKYRMDRDFFIKTFAELNISSDSSSVCRKRKLKDDYISIPSYATTDAISYAATYAISYATTNATTQQQLIQQQLMQQQYLIEDPHGNNIAKLPTTKQKEELYLKKAKHIYLKSTSNQKRNSLAGEDCYFNNKLSYLLTDNGLPNGISDEVQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPQ5</accession>
    <name>A0A8J4UPQ5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="2 9">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_009149</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="2 9"/>
    <dbReference type="EMBL" id="AJWJ01000644">
        <property type="protein sequence ID" value="KAF2069541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UPQ5"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000013">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000046">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008352">
        <property type="entry name" value="MAPK_HOG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01773">
        <property type="entry name" value="P38MAPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="301"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="213"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="672"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="66"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="213"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="672"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="713"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2069541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="724" mass="85125" checksum="30FA7CA4841AEF2B" modified="2022-05-25" version="1">MEPHYDHFQAQLEADRYSQPPQSMFNATGDIPIEYLGPDRVQQQHHQHQLLQQQQIQQQFQQEQQLQQQQLQQQQYQQQQQQYQQQQQQFQQQQQQIQQQIQLQQQQMQQQPQISSDNLNQLKIQLEQLNLQIRQQQYQQQQLEAAQQQEQERQQKIQMIQEQQQIQQLLEQQAQAQQHQQKIQMERQQDEIIRRQQVLEQQLIDQIKEKELNNNNNNTINNNINKIPSPPSTSLESSTSGVATTTTTAAPSSAPSKEEYLTTTITKLDDASSYTPLTQASMNETVSYFVYGSKFTIPKRYSLVKCIGYGAYGVVCSAIDHVTNEKVAIKRIGYIFDNLKDTKRIYREIHLLRNFKHENVISVKDILKPQCRHTFEEVYIVSELMDTDLHQIIATPQDLDNEHYQYFVYQVLRGLKYIHSAGVIHRDLKPSNLLINSDCFLKICDLGLARVVDAAHVGFMTEYVATRWYRAPEVILSWNKYTKAIDIWSVGCIFAELFGRKPLFQGKDYIHQVKLIIETIGSPTEDEINQIPTEPARAYIRNMGYKEKKQFSQLFPDADPLAIDLLEKMLCFDPDKRISVEDALNHPYLASLHDPADEPTCNHNFNLNFEAWDLSKEILRELIHNEMLAFHPDNETDTTMNIENPQQTFQPQQTQQAQQQAQQQQTYQPQQQQQQAQLQAQQQQQQQQQQQQQQQQQDQFTNNPMERMKFLMEQTNSLERQLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQY7</accession>
    <name>A0A8J4UQY7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Heat shock protein 70</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_007311</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000382">
        <property type="protein sequence ID" value="KAF2071371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQY7"/>
    <dbReference type="OrthoDB" id="3789372at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10233">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000172">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000001">
        <property type="entry name" value="heat shock 70 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000134">
        <property type="entry name" value="Heat shock cognate 71 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000016">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000026">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="610"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="613"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2071371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="644" mass="70769" checksum="06D93AF312022E5E" modified="2022-05-25" version="1">MSSIGIDLGTTYSCVGVWQNDRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRKFGDSVVQADMKHWPFKVVAKEGDKPYIQVEFKHETKTFSPEEISSMVLIKMKETAEAFLGKTIDKAVITVPAYFNDSQRQATKDAGTIAKLNVQRIINEPTAAAIAYGLEKKGAGEKNILIFDLGGGTFDVSLLTIEDGVFEVKATAGDTHLGGEDFDNRMVNHFVEEFKRKNKKDIMGNQRAVRRLRTACERAKRTLSSSSQASIEIDSLFEGIDFYTSITRARFEELCADLFRGCIDPVEKVLRDSKLDKKTINEIVLVGGSTRIPKVQQLLQDFFNGKELNKSINPDEAVAYGAAVQAAILNNEGGEKVADLLLLDVAPLSMGLETAGGVMTSLIPRNTTIPCKKSQTFSTYSDNQPGVLIQVYEGERSMTRDNNLLGKFELSGIPPAPRGVPQIEVTFDVDANGILNVSAEDKTTGNKQKITITNDKGRLSKEQIEKMVADAEKFKSQDEAQKEKVELKNKLENYAFSVRSSIRDEKIAGKISAEEKSTIEAAVEEAIKWLETSNSAEKEEFQHQQEKLEKIVNPIMTKLYQEGGMPGGMPEGGMPGGMPDMGGAPNKSGTVDELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4US80</accession>
    <name>A0A8J4US80_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">RNA-binding region RNP-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_005390</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000214">
        <property type="protein sequence ID" value="KAF2073291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4US80"/>
    <dbReference type="OrthoDB" id="29221at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041591">
        <property type="entry name" value="OCRE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13948:SF3">
        <property type="entry name" value="FI21118P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13948">
        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17780">
        <property type="entry name" value="OCRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="158"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="14">
        <location>
            <begin position="238"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="664"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="domain" description="G-patch" evidence="13">
        <location>
            <begin position="745"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="270"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="540"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="702"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="94"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="270"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="280"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="540"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="714"/>
            <end position="730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2073291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="830" mass="95500" checksum="E7CDC6CBA64778D4" modified="2022-05-25" version="1">MHKGNNTSSSALEEEEGSYTPSERASYYRDSDGRRPRSRDREYKSEKRSNSRSRSRSRDRDREKTKDNRYHDRSRSRSRSRSRSSQSRSRSRSRSKDRYSQKRYTNRDYSDIDYSYNKKSYHRSSSRSRSRSRSKSRERRGGRDNRRKPINLDEEPTSTIMLKGLNYITTMEGISDSIKVYSAYESISLKLDKKTGKSRGFAFVEFKNVEMAVSFMKETNGSLKIDDRKIHLEYALPEIQYEWLCDSCNASSFAHRPSCFKCKQEKPEKPRFVSTEKDNSNNNNSNDSSSAPVPSNTLVILDCKSEVTEDILEGIFSQFPRKHKEIRISRKKSSVAFIEYYAVDDSIEVYKHIQLYGGVNINQQMVSVYYSKSSDSDPSHTSINGLSSLDMHQWLKNYGNSASIPNNSNIKIPDGYIIEPNSGFYYNSSTGYFYDPAQYTYFYWDQNLQGYYYYDNTTNSYLPFAPTTNNTTTATSTTITTTTTTTTTTTTTTNNAATKDGKKKLVFQPSKYQGIKKKGEMDIEKWNQKGKLLKEAFDEEEKKYNKHIEQQQKHHQHQHHEEPQQQEPQQQQQQEEEYDPSNPSEGVIASSIQQQPTPVNQPIKFSINNNSNTTANQPIKFSFGKPSSTAPVKVVAPVITKRPQFNDENEQQQDPTPQIEPSDIVCKICDRLFISHEALTRHENESKLHASNLEKLGIFKKKEKPTSSSSFSHNNNNNNNNNNNNNNNNNDNEDRKRKPDITFGENSIGFKLLKKTGWNQGEGLGKNKDGMTSAIQVEYRSEKQGLGSDPVDPKFAILPGDDYQTKLRKKANQRFHSLGGKLDQDNPFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4USI7</accession>
    <name>A0A8J4USI7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chitin-binding type-4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_005016</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000190">
        <property type="protein sequence ID" value="KAF2073666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4USI7"/>
    <dbReference type="OrthoDB" id="17911at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.50">
        <property type="entry name" value="chitin-binding protein cbp21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004302">
        <property type="entry name" value="Cellulose/chitin-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051024">
        <property type="entry name" value="GlcNAc_Chitin_IntDeg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34823:SF1">
        <property type="entry name" value="CHITIN-BINDING TYPE-4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34823">
        <property type="entry name" value="GLCNAC-BINDING PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03067">
        <property type="entry name" value="LPMO_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035284533" description="Chitin-binding type-4 domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-4" evidence="3">
        <location>
            <begin position="28"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2073666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="246" mass="26747" checksum="912B7FCBE3437655" modified="2022-05-25" version="1">MKNLTICLLILSIISFTNGHGYGVGDLMSRQIKCTTSPSFSIWWPEDGTGIANLACRAAYQHVATKTGSADAARYQFVQKNEYSVLIPNYAAGYSALTAAVPDSLCSAHAVNNAVQFGDKSGMSIPNNWLLNTITVPSTSTASKDVVFEFCATAAHNPSFWEFYVTNSGFNIQTSNLTWSDLTLIHSTGDNLPVTNTNANCEASKAYKIPITLPARYQGATLLVRWQRVDPVGECFINCSDFIFSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVI2</accession>
    <name>A0A8J4UVI2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pseudouridine synthase RsuA/RluA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_002092</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000055">
        <property type="protein sequence ID" value="KAF2076606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVI2"/>
    <dbReference type="OrthoDB" id="418349at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02869">
        <property type="entry name" value="PseudoU_synth_RluA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluA-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050188">
        <property type="entry name" value="RluA_PseudoU_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600">
        <property type="entry name" value="MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600:SF86">
        <property type="entry name" value="PSEUDOURIDINE SYNTHASE RSUA_RLUA-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Pseudouridine synthase RsuA/RluA-like" evidence="5">
        <location>
            <begin position="137"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="86"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="338"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="522"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="354"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="375"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="532"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="557"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="578"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="594"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2076606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="610" mass="68682" checksum="88B6D630130597F0" modified="2022-05-25" version="1">MYTLEIIVPKQHVPEKFDKFISSSRVVVVETGELVKLSKSSVPKIVTSKSVSVNGALLKDKSIKVRPGDHIIIKIANIDPYKVQNQNEQKSKPVHHQQQQQSQPQKSNVDTAAPAPTELKLVATDIPLNILYEDEYISVINKPAGMLVHPVQHSTTLSSTSTAAIQNSNKENQTHTLVNALIHRYGLENLSTCGGADRPGIVHRLDKDTAGILIIAKNDDVHEKVKKLIEDHTNSMINTVNEYGVSTTDPTRKKPKITIQKKYHCLVLGRVRTNHGFISKPIKRHPNDINKMIVDEEGKESITEYKVLKVWDNLDLKVYDQTKNRNLSTNSTKNEILSEYDNVPMMSQSMSSSKNKKKAQKKKTFNSMSLLSVDSDSEDEDKEDEEDQDESEDEEEESSEEESESESESEQEESDSDNDYDQDIKDLIQKTKRIGFNSFTLLEVTLHTGRTHQIRVHMAHEGHPIIGDPIYSSKSKQFMVPYLLLASMNIVFKHPIDATIKEFSIEHPEHIKSFIDSLNKEEKEKENRQLKSASSSTISSSNSSSSSAKPSKQSINKPSSKLPTTQASSKHPLPPPQSSKTRSTAQSRKGSSVMKKEDELDKEFLEFYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWD5</accession>
    <name>A0A8J4UWD5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SP-RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_009200</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000654">
        <property type="protein sequence ID" value="KAF2069478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWD5"/>
    <dbReference type="OrthoDB" id="21045at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16650">
        <property type="entry name" value="SP-RING_PIAS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF4">
        <property type="entry name" value="TONALLI, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02891">
        <property type="entry name" value="zf-MIZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SP-RING-type" evidence="6">
        <location>
            <begin position="404"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="286"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="541"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="299"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="315"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="542"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="597"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="635"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="654"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="672"/>
            <end position="696"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2069478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="746" mass="82556" checksum="66FEB9EEEFEB9C30" modified="2022-05-25" version="1">MTDNLTTSEWEAISRKVQRSDLRDRFRSPHLLGTLHVPELKEILKVLRETRRGVSLTGNKPDLIQRIVRTLNPNTSDSYLLPTQQRHNNHQHHNHHSTPTTVIQPPISFNAKQGASMPHGGHLNFGYNSLSTLTEAQLIHFRDRQNPYFKEIKKLSTTYFKDSTNISFHIDDDTMKLLEMPTKEYGVQIRLFNRNTLSDILYQGDVRMSINGKSPSIVIKKIRYQTFSKPYIIQKSIDITEHVKKKDNRLYFVFPKNEGFLIVQLVQSRTTQDIVDSIYLKSLPPPNIVEEATSNSGNDDDKMDTTEDKQPNGATDSTTTTTTTTTTTTTNGTTGESTSNSSSSSNSSSSSSSTLNGKTTTTITNENTQTNGTSSSSAATTTTTTPAPTLAPLPPPALTKKVVEDDDIEKGQEDFRIRCLLSYQRMKYPAKTVDCPHIQCFDLAAFIEYSNQQQLYNCPICDRIAPPSMLIVDLFYKDLIANSPEDCEYITIFPDKKWEYKMEQAINLNEESPTLNFKSNSNGGGSNDIIYLDLDDDDDDVPVSSTTSTTTSLPTTTTQTTILQSSLPSSLAEPTLIPTPAQTPVFGAPTQNRLSQSTPGTTTASTTEAATTTTTPLSSSTPGTTTTTPSVATPVPSPPPPPTITIPSTPTPIPALAVPLTSTSTPSSSSIFEDDDEYNQEEEEELDDDSNSDDEYGYIPYYREREAMFSESEDSDSEVSDSYFNFAPVRLNIDPNDVILISDDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXE2</accession>
    <name>A0A8J4UXE2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000231</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000004">
        <property type="protein sequence ID" value="KAF2078481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXE2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="498" mass="57249" checksum="CB1A7E3ED7EB9441" modified="2022-05-25" version="1">MTESFYLIWRNQYIRKLIRNRVCQNLVINVNENYLIRNHQYLSLFTNRDKLDYNIGIRFEGRDDDYLDINSNNRDLINVVDFKKDTGNFNLDIILDCVQKLSFSTNSNTMRATGKLPPSITWLQLMKDDFYCIKPLAQQILSNLPDNLQWLELGSHLQYNFTSPCIMPQSLTDIKSSSYFYYQNLKWFVVPPNKVYKSCVLYIDSMESFEWLLENKWICNVEIDPNVLIMLMGQYQLPSHVTKVNVNVGIVRDISFLPQTLESLEGIYGASFSHLTQLKVLKIYDISVEKLEKGVLPPLLQELLIIYNYPLEIDVLPPHLTALCLPIYDCPLYTNVFPASLTSINLGSFKQPFHPFVLPNKLKSLSMQKFTQPTFPPNSLPLSLSDLALDRFKGSFDQCQPLDNLQSLKICSLVPSLATLLTNVKKLDIEVAGDNDDDLSGTCLANTSIKSLYLKFVFQRTLYPNTFPPTVRYLTLINADLKSDDVIPSGCVLKNEYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXH9</accession>
    <name>A0A8J4UXH9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000003">
        <property type="protein sequence ID" value="KAF2078556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXH9"/>
    <dbReference type="OrthoDB" id="299565at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006540">
        <property type="entry name" value="Lactococcin_972"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09683">
        <property type="entry name" value="Lactococcin_972"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="145" mass="16530" checksum="0506F4DFAC20EF8F" modified="2022-05-25" version="1">MWIDIFFGFFLCVCIIWIPQYFGQLLLSQQPPQPQQPQNNPQPGLFYLVLQLFKQIDFLAALRLLINVERNKTVILKPHPGAVWISDLTGDSVVSAYYHPTKRHTSTTIGALSMQRSTVGPGEWAYSRQTRAASGNRTFYNTLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXP0</accession>
    <name>A0A8J4UXP0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000405">
        <property type="protein sequence ID" value="KAF2071175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXP0"/>
    <dbReference type="OrthoDB" id="1938039at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="23"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="123" mass="14514" checksum="64F0AFC763E06434" modified="2022-05-25" version="1">MNIDDLYGDIKINQSFHSDEEDDEDQKKLIDQQKSRIQELEKLVELKDKEILELSLTIKGKDETIDTLSKQKDSSLKANEILCKNISCLFKTSLLEIQRKDKEISSQRDTIEQMNQQQHYQKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYZ4</accession>
    <name>A0A8J4UYZ4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein L1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006438</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000297">
        <property type="protein sequence ID" value="KAF2072238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYZ4"/>
    <dbReference type="OrthoDB" id="10251727at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="385"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="449"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="273"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="449"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="482" mass="53033" checksum="28EA48C515946077" modified="2022-05-25" version="1">MPTKATSTPTTTTTSKVQLVNKDKALEAINNLFKYIKGKNATDRLNILEESKKFYVNFTTTKYNTEIQFKPYHITLPNPLYQNTVKTFVIVKGNKEEAEEFKNVLSQLGQAVDVISQKEFGKTYQTNEQKVSLRKQYDEFIIDSNYRAKVQYLLGKEFVQSAKTPKVISIDITKPEEFVNQLNQLNRKALIKSFGKKLLFTIPFGDSELTANQLIENLAVVVNEITEKLPEKLASIEALSVETLSLYLPFYTNITVPSNISLNKKGQVVSTLPTTTTETTTTTTPTKENKKRAIEEKPVEPSTPVVSSKKAKVESTPVKAAEVQKQTPVKKAETPKKVEAPTKKAAVEPTPAATPKQTPVKKAETPKKVVEKVETPLVEKVETPVKKATVEPTPAATPKQTPVKKSTPVVEAPVVAEPVATKKVVKKTVTATEAPVAKKVVKKTVATEATTEAPVAKKVVKKTSTATATESPVAKKVVKKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZU4</accession>
    <name>A0A8J4UZU4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NlpC/P60 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_010361</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C40 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001062">
        <property type="protein sequence ID" value="KAF2068312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZU4"/>
    <dbReference type="OrthoDB" id="20888at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.10">
        <property type="entry name" value="endopeptidase domain like (from Nostoc punctiforme)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000064">
        <property type="entry name" value="NLP_P60_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051794">
        <property type="entry name" value="PG_Endopeptidase_C40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47359:SF3">
        <property type="entry name" value="NLP_P60 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47359">
        <property type="entry name" value="PEPTIDOGLYCAN DL-ENDOPEPTIDASE CWLO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00877">
        <property type="entry name" value="NLPC_P60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51935">
        <property type="entry name" value="NLPC_P60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035161042" description="NlpC/P60 domain-containing protein" evidence="5">
        <location>
            <begin position="24"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="NlpC/P60" evidence="6">
        <location>
            <begin position="45"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2068312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="178" mass="18606" checksum="9724515424C538CF" modified="2022-05-25" version="1">MKSISSILLVVCLIATAFYSVQANASMFVDVTNEIKSMNFTALPASTCAKAASQAKHYASCGCPYVWGGTSCGCGGSGGMDCSGLVYTSYREAGYSGITRVTTTQVHQGSGCNAGCSPSNTHNCKVGDLFFYCFGSGCPDHVLMYVGGNQVAECSVPGTDCSVHAPYSENYQTCRSFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V056</accession>
    <name>A0A8J4V056_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Derlin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_004708</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May be involved in the degradation of misfolded endoplasmic reticulum (ER) luminal proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the derlin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000172">
        <property type="protein sequence ID" value="KAF2073963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V056"/>
    <dbReference type="OrthoDB" id="1716531at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000016">
        <property type="entry name" value="Derlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007599">
        <property type="entry name" value="DER1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11009">
        <property type="entry name" value="DER1-LIKE PROTEIN, DERLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04511">
        <property type="entry name" value="DER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="122"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="224"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2073963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="258" mass="30348" checksum="16158D9E42B700BF" modified="2022-05-25" version="1">MAQPFEDWYKNLPIVTKLYMTGCVVTTIGVYLELVHPLQLYLNFPLITKRYEVWRLVTNFLFYDHIGLNFLFHMYFLVRHSRLLEEGSFRGRSGDYLFMWIFGASLLLIINAILFYSKLYTSSILFLAPSMAFMVVYVWSKRNPNIHISFLRLFTFSAPFLPWVILTIGYLFHHSLANDILGIVVGHIYYYLEDVYPTVSNRRLLKTPSILKSLFDNNNHALDPDQQRHAPVANWAQPEQQQQQDQQQQEQEPVAVNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0T3</accession>
    <name>A0A8J4V0T3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">SNF2-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_003906</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000129">
        <property type="protein sequence ID" value="KAF2074803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000015">
        <property type="entry name" value="lymphoid-specific helicase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014012">
        <property type="entry name" value="HSA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029295">
        <property type="entry name" value="SnAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07529">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01314">
        <property type="entry name" value="SnAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="599"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="907"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="156"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="519"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1197"/>
            <end position="1364"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="467"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="523"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1208"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1269"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1322"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1355"/>
            <end position="1364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2074803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1364" mass="157338" checksum="F0D064D347254C06" modified="2022-05-25" version="1">MSPNIFLEDKTNSPVEGSSKLNGYNHIDHQNMNTTTTTTTSTNGSHNTIFNDVNYLKILNVIKDMKLKGTEESDPQYITLLQIAKLYQIQYNKNNNSSSNDDVMQLDNGISNNNNNSEEEKDIFRAQLESYKYLSRSLPIPPKLLSTISSVLEYDQDKDTTTTETPIQQTQKTKDNNEEEEESQQLEENNMKLRYYPRGVLKLDSENNVIINDYGMGFLQQQQHLYLQQQQHHMFQEQYLDEQQQQQQQQLLQQKKHQSQQEKPNVKGLQESLVSIDERDLRMHTKIHYRIDELKEMPLNHLPVEMKLKAQIEMKQLRLVDLQRKLRIDIAGEINNQIFLDSALETDLLVKPIPRVVSKPTEIMYDNTATQLPESILVTHKHKFLEAVLAHQKQFKDYHTDNAKSRNFVVKQIARYHKEREKREQERLKKERIRLLRAQDTQGYRDLLAKTKNDRLEMLLGQTDELLSSINNLMKKEEIEKKEREKERERERLEKEKLEKERLEKERLEKEKLGIVDDESNITDKSTTSNNTTTTTTTTTSPIPNPIPIQPIANISSSLTSTTTLIQKKSLLDEPIEQPEIMTGGKLKEYQLTGLEWLVSLYNRNLNGILADEMGLGKTVQSIAFISYLYEKLNVREPFLVVAPLSTISNWSSEFARWSPKLPTIIYKGKPDERYEQARQIPKNGFVVVITSFEYIILDKKRLGRVQWCYIIVDEGHRIKNKDSKLSVQLRLYHSKHRLLLTGTPLQNELGELWALLNFLLPTIFNSLDTFENWFNAPFAGAKGGKSVIQVNEEESLIIINRLHQVLRFFLLRRLKSDVESQLPDKKEKVIKCNLSAMQIVMYRTLLEHQVLLIDPESETFKKNKMKRRGFNNIVKQLQKICNHPYLFKDEWAIDQDLIRASGKFDTMDQILMKMRASGHRVLIFTQMTEVINLMEEYFSLKEWDYLRLDGSTKPDERSHLVVEWNRPDSPHWIFVLSTHAGGLGMNLQTADTVIIFDSDWNPQMDLQAQDRCHRIGQTNSVSVFRLISANSIEEQILARATDKLEIDAKIIQAGMFNTHSNDQERRAKLEEYLHGFPRDTTEEAPTDLEEINRLICRDDEEFAQFQEMDKEKAKIDKQLYKNKVPSRLITEAELPAWMLESPIEEEEDTTFGKKRAAAISAANNPVDDLTELQYAKMVEKGMSLEEYKKYLVDKREKRNGGGNDQFSSDDDEDEDIEDESSEDEKKKKKKKKIVIKNSSSSSSTPKRVRGGASKKAAATPSKLNGNSTDQETTTTTQDGQEGDQVDVGGVDINTTTASGDEAMDSSVNGVNDDTGDETKTSKQRGRKKARVTGGSDEYISALPVHSNEPMTTRSGRKLRTVQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2L8</accession>
    <name>A0A8J4V2L8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_009784</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000805">
        <property type="protein sequence ID" value="KAF2068894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2L8"/>
    <dbReference type="OrthoDB" id="10009520at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20346">
        <property type="entry name" value="BRcat_RBR_ANKIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16773">
        <property type="entry name" value="RING-HC_RBR_TRIAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000019">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045840">
        <property type="entry name" value="Ariadne"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048962">
        <property type="entry name" value="ARIH1-like_UBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19422">
        <property type="entry name" value="Ariadne"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21235">
        <property type="entry name" value="UBA_ARI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="283"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="287"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="125"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="19"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="48"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="125"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="162"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2068894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="680" mass="77797" checksum="4112DEFDB628915D" modified="2022-05-25" version="1">MFYKLKKILSPNGKKKKSSSSSASSNVVKNSNENSSYNSYDDIDNKNYKSQTSSSSLDASSEVENENETKINPIITTTTTTTTTTTTNSLNDDEKKPPTTITITPSSSFLRTVSSQLLNIKKNISSNNNSSKIDNNSPKTTIKVNGDEDDQDDACNQSKDDNQDDETNESFIDNDEEYSDNDDDDDDEFIGFQDIEKESDKNFNCLSPEELKLQQQKEIKEIAELLSIPMSSAITLLKHFNWKKEILILKYIESPKEISKELGIEYNSNNNNNNGNNNNNNDTIISCSICGDENNLQEFTKIKCNHYFCNDCWGGYLVLKITEGEATIRCPSHKCNSVVQDTIIQRLVAPVLYEKYQSFATKKFIQQNQKHIRYCPTPGCDNTITLRNDTVSSTTSVQCSCGFKFCFQCHRQSHLPATCEQMALWETKCQDESETSHWKTANCKECPKCKVAVEKSGGCNHMVCKHCQYEWCWNCLSVWKGHNDFYVCKKTLSSKATKKNQDALKEIEKSKLELSRYLQYYERYQFHEKARHQEKSIREQAQTKMRELERSSTWAEVQFIEKGIDQLLDCRNVLKFTWIYSFYSFPNSNISISTPTPSTTNTTTSTSTSQDDNAKYLFEFLQNDLEKSTEKLSDLMEDILKKSNTLDQPLRLEIMNQISLLKSKKDGLLDAVSKDSLFEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2Z3</accession>
    <name>A0A8J4V2Z3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_002354</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="AJWJ01000064">
        <property type="protein sequence ID" value="KAF2076348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="266718at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007224">
        <property type="term" value="P:smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22983">
        <property type="entry name" value="PROTEIN KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22983:SF6">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="6"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="249"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="493"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="545"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="574"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="727"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="926"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1055"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1828"/>
            <end position="1852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="254"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="269"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="285"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="498"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="552"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="574"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="629"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="727"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="747"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="941"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="966"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1059"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1088"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1834"/>
            <end position="1847"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2076348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1928" mass="217239" checksum="F7AE22DD5F16DD8E" modified="2022-05-25" version="1">MDHDNYHIIDLIGEGCYGKVYKCRKKYTNKIYALKVIPKKGKSIKELNSLRSEIEIVRNLSHPNIIQFYDSFETDHDFHLVTEYAYTDLYQIIQEEEEKHVFNNDSDNSNTASKSPITTRKDKLTLSRDMIQSICYQLVQGLYYLHSNRVIHRDIKSQNILISSTGRLKICDFGFAKTISSHSVMLTSLKGTPLYLAPEILKEQPYDHKADLWSLGILLYQLVTGKLPFVTDNIGSLIHLVLDNTLKLSPSSNKQHHKKRHHQRHQRNHSMASSSNRLSTSASDNGDDEDDDDDSNDDDNDDEYSNDDDLNNQNNSLPYEAIEDIDIMSLLKGLLCKDPEKRISWPEILSHPFIASFKVSPISLNLFLHRLKPTLTNSKVAKQQLYQYKQHQLDVIEKEREKKRKSKKKILTFKSPSTSITATPPVAVVIESEMKQDIHIVNNNNNNSNGNIQIDHLQHLQHLHSHQVSYSIPSTISSLKLEPTLQSGELYKDEQISDDSNSNSNSNNQRDGDDDEKITNFLSPMSGIRIDTPPMSAVQSFRSKQLLEEDVSPPSTSSSSSTSSCFSSVTSASVSFTSSPPITSTATPAPNEAFSSPPTNSLPPKTPIHHPLPAAQLALPSRHPITSPGSQQHQQHSSGNSSGNGGMVRSISNGSISATSPLAKPLYMSMKPAPVLAIPQSRPQTPGVLISPKYLFQPPQTPNIFELQQQFQQQQQQQQNAYLSSNVNSINSTNNNNNKNKIPPLSTPLTRPQTPNVLRPQTPNIYRPQTPKISPKSFNYSTNNNNNLFFSVMKSNQILQQQLQQQQQQQQQQAYQLQSPSIYQQPFYPQQQQQQQQQPSFNDFHFIGERIEIESNDGIEGYDFWSYHESLSSQSEENSIKLLGDKSFIQKIVYHLQASCTLQIEVLPVNSILRTFSNLMRAHKNISKSSGGNGSSSGNNTELTQEQVSNSVKTPPPTPYIQYSFNQNQSGNNGNSHGNANANTYCTDLIYKASNLHLLINLLSNILASTLNGNGGMPQQQVLNSPFINLVECLDTIHIFFQTIQPSLSLLIPPPSPISSPHTPLTPNTPTSSPTKKQFMMHAHSNKQQHTQQQQQQLQQQQHHQHHFNLFLSIVNHLLKNISLYNANIQTSIINMITCFFGKLGDAPLNLFDIYKKILENSDILSNLCHYLFIHVHLNNSKFTPAEIVQQREFVAIIKCLAAFLNTCPTAVMEFPMDAKRGNYYAHQQHVSSVLCYHTAGNIIGDGLSVGPLVSILFEALENPLLQQDVLSLFLHCSRASRLLVEAIHKEEKHNLETNATNKKSLFDYLNDTNTNSNSLILLLLGTILMHIPESIDWIIQFDIVQITLTYFCCNDHRLSSCASFFVGAFLSEAMHVEKEQLNNNNSNSNNNGDDLVDAADNDEEISMIIDQVIESIPMKFIRRLFGSKRVPGQTMRDLEGGCFGRPYLGLLDGAACILLRLIKRGDKEFLETMLESGIWEALCHQISDSTTEVELSPHGVIYALRVVYEVLSKNTAQHISYLVKNNLLVSLCQLLESSHLERLKDWPSIQLGGSSGINALVTQIICILYLPLSSRSKASLDASLLELIHHIMSSQQLIRNVIHLLTLLPLESLDLPLGLLSNVILEDPQFSTQFVQFGGLDPQYIHLLLNTEKNSSILLVDSLIILSQLARVSDENYKAIHKADIYHFLKKLLNHPDPNVRSKTCNLIGNLFKFNDYFYQPIRKNGILPLLVSRCSDPDPNTRKFASFALGNAAFYSDLLYSDLADSIQPLTTLLVDLNEDEKTRSNVIGALGNLARNSHILCTTMIEAGVLNAFIYYLNDDNVDDDNIGTNDQKEEKEKEKENNGKQRQHQHTNLLKGVLFSLGYFCLYEQCRNIIKIQFPYLNNLLDHLLSSNQEDQSVISKYIYRIKKMMSFENSDSPRNDLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4U9</accession>
    <name>A0A8J4V4U9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">phytol kinase</fullName>
            <ecNumber evidence="13">2.7.1.182</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_004761</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>phytol + CTP = phytyl phosphate + CDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38055"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17327"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:75483"/>
            <dbReference type="EC" id="2.7.1.182"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="12">Cofactor biosynthesis; tocopherol biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the polyprenol kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.182" evidence="13"/>
    <dbReference type="EMBL" id="AJWJ01000174">
        <property type="protein sequence ID" value="KAF2073937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4U9"/>
    <dbReference type="OrthoDB" id="5673at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010276">
        <property type="term" value="F:phytol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039606">
        <property type="entry name" value="Phytol/farnesol_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32523:SF8">
        <property type="entry name" value="DOLICHOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32523">
        <property type="entry name" value="PHYTOL KINASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="42"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="191"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2073937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="240" mass="27236" checksum="58FAE365C563030C" modified="2022-05-25" version="1">MEIKMILVYGFFKTLAICLVWLKITQFLTKNRITSNPTSRKIIHIGTGFIYVMCWSFFPERDPFSRYAVALVPFLISLQFTLIALGYIKDRETVNSMSRSGRAIELLYGPVSYGIVLTLCTVWYWTESPIGVTSLCILCFGDGFAGLFGALYGRKRLPINSNKTYVGSISFLVFSFFGTILLLHLMQMLGLMYYLSITSYIPSLAITCFVCCLVESLPIEDWDNVSVFLASINLLRFLGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V536</accession>
    <name>A0A8J4V536_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">U5 small nuclear ribonucleoprotein 200 kDa helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_007184</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000369">
        <property type="protein sequence ID" value="KAF2071489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5575at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000393">
        <property type="term" value="P:spliceosomal conformational changes to generate catalytic conformation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18021">
        <property type="entry name" value="DEXHc_Brr2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000002">
        <property type="entry name" value="Activating signal cointegrator 1 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000133">
        <property type="entry name" value="Pre-mRNA splicing helicase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000004">
        <property type="entry name" value="RNA helicase, activating signal cointegrator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000368">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein 200 kDa helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003287">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein 200 kDa helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000012">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000024">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000004">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000001">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000062">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000254">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF4">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="11" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="110"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="761"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="966"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2071489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1582" mass="180879" checksum="00F82AEF789E7353" modified="2022-05-25" version="1" fragment="single">SLTNKQISETQIIVTTPEKWDIITRKSGDRAYTQLVRLVIMDEIHLLHDERGPVLECIVARTLRTIEATQEMVRLVGLSATLPNYEDVATFLRVKPEGVFYFDSSYRPIPLEQQYIGISDRGIKQLHLLNDITYQKVSERAGENQILIFVHSRRETGKTGKDLRDRLVQDDYIGKLIKTMGIREILREEAKNTVKNAELKDLLPYGIGIHHAGLSRSDRTLVEELFADQSIQVLISTATLAWGVNLPAHTVIIKGTQVYNPEKGWTELSPLDVTQMLGRAGRPPFDKEGEGIVITSQQELQFYLSLLNTQLSIESQFISKLADNLNAEIVLGSITNIQDAVYWLGYTYLYICMLRNPPLYEISYDEIEADPELEQRRTDLIHSAASILEKNSLIKYDRRSGKLISTDLGKVASHYYITNASMSIYQEHLKPTMSDIEFFRVFSLSYEFKNVVVRDGEKFELEKLLERVPIPIKESIEDPSAKINVLLQTYISNLKLEGFALVVDMFYIAQSASRITRALFEIVLKKGWAQLAKKILNIAKMIDNKMWASQSPLRQFHEISPKILNQLERRGIPIEDLYEYNSQQLGNAIQNAAEGKKLFKLIHHFPRVELTAHVQPFLRGLIKVELTLTPDFEFDEKYHNNSIGWWIIVEDVDGEKILYYEFFSLKKRMMTSDQMVSFTVHLTEPLPPQYYVRVISDHWIGAEYNLSISFRHLILPEKYPPCRSLLDLQPLPIDVLKDSRAESLFKPTFKIFNAIQTQVFNCIYQSNESAFIASPTNSGKTVIAELAVIRAFQQKPNGKVVYLAPMQELASLRFRDWQYKFGKVFGLTVSELTGDAVTDNKILDKSNIIVTNAEKWDILSRRWKQRKALQAIDCLIVDELHLIGGSYGPTMEVVVSRMRYIATQTGNPIRIVGLSSSIANARDLVLWIGASPTTCFNFHPNVRPVTLDIMIQGFDFPHFNARMLAMTKPTLSHVSKNSAGQSIVFVPSRKLSRSLAKDLIVQVTSESDLHHKKYLNISEQDLTMYLENVDSIALKQTLEYGVAFYHEGLTDKEKRVVENLFKSGAIKVLIATHSVCWCFDVYASLVVIMGTQLYLGKDIRYVDYSMNDVLQMIGRAGKQNLDKHAKCVLLCHTPKKEYYKMFLNDPLPVESHLDHCLHDHFNSEIVTKTITKKQDALDYLTWTFYYRRLNQNPNYYNLSGVSHIHLSEHLSELVENTLLDLEQSHCINIEEMDEEDGDNKISPLNLGIIASYYYLKYQTIELFGSSLKATTKRKGIVEILSNAPEFAVIPIRHREEQVLAKMAAHLPLKIDNPNFSLVSTKVNILLQCHFSRKPISADLYQDQKIVLENATRLLQAIVDVISSSSWLTPALAAMELSQMITQAMWDSDNTLKQLPHFTKQRVDDCVQAGVESVFDLMALEEKSRDQLLKMNEFEVTDLIQACMRYPDIDISYQVQDEDDLYADSAMSIEVVVERDIDEENINDSVNIVNAPFYPKEKVGGWWVLVGDPKTNQLYSIKRISFTKKTKVKLDFATPPVGSHTLTLYLMSDSYSGCDQEYEIQLNIKPAQIDEDDEDDENNEMQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V595</accession>
    <name>A0A8J4V595_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000801</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glyoxalase I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000016">
        <property type="protein sequence ID" value="KAF2077923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V595"/>
    <dbReference type="OrthoDB" id="5371818at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07253">
        <property type="entry name" value="GLOD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050383">
        <property type="entry name" value="GlyoxalaseI/FosfomycinResist"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21366:SF14">
        <property type="entry name" value="GLYOXALASE DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21366">
        <property type="entry name" value="GLYOXALASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="2">
        <location>
            <begin position="5"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="136" mass="15248" checksum="CCE112AC5CAE565C" modified="2022-05-25" version="1">MKITRLDHLVITTKDISKSLYFYTNILGMELDENLFNEKKRYAVKFGNSKINIHVRAGEFQPAAKNPTIGSGDLCFIVDGDINNIKKEVESKGITIEEGVVKRTGALGPIDSIYLRDPDENLIEISTYNIDVAIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5H4</accession>
    <name>A0A8J4V5H4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Malonyl-CoA decarboxylase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000547</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000010">
        <property type="protein sequence ID" value="KAF2078163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5H4"/>
    <dbReference type="OrthoDB" id="426718at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050080">
        <property type="term" value="F:malonyl-CoA decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001294">
        <property type="term" value="P:malonyl-CoA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.150">
        <property type="entry name" value="Malonyl-CoA decarboxylase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038917">
        <property type="entry name" value="Malonyl_CoA_deC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007956">
        <property type="entry name" value="Malonyl_CoA_deC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042303">
        <property type="entry name" value="Malonyl_CoA_deC_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28641">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28641:SF1">
        <property type="entry name" value="MALONYL-COA DECARBOXYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05292">
        <property type="entry name" value="MCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malonyl-CoA decarboxylase C-terminal" evidence="1">
        <location>
            <begin position="214"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="477" mass="55408" checksum="D7539615FE97D341" modified="2022-05-25" version="1">MMFSRNARNIKHSTTCLLKHFDIKHSRLFSTTTTAKSFASRVDKILIKNILNSNISNSSSNNNNNSNFSYVQKHYTTIVNNNSSSNNNNISMNDFFNNYQQNTIDKPLESKIDLTSKFLLQFKNQTNNNDSWDYSSIESFYSKDNQVTPTVLSNFLLIRDDIEKYILLKMNSNTKDTPTILGQDLILFDTLLGTLIKKNMKVDDLYCTPLQLSMGKEIIHQIINNEAVHPYQDKQNYIDELKQRISKNKMCLVLFHPSMKNTPLMSLYIVLKEKVPDGMESLEEIPKQESDICCAIFYSISSLHKGLKNVDLGHILISKALNYIQSNFDKNHTWTYFTLSPLPTFKSYVKKLSNKDSNLQKVIQEFESKAQSEKINMLDRIRSILEPLAYQYIQEKRKGGKTLDPVCNFHLKNGANIYRINWKGDTSEKRLEESFGMMINYEYDTKIKTQNSINYKTNHILSENQLFKDKLNKMLNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5U2</accession>
    <name>A0A8J4V5U2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Squalene monooxygenase</fullName>
            <ecNumber evidence="13">1.14.14.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_006564</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the stereospecific oxidation of squalene to (S)-2,3-epoxysqualene, and is considered to be a rate-limiting enzyme in steroid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>squalene + reduced [NADPH--hemoprotein reductase] + O2 = (S)-2,3-epoxysqualene + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25282"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15440"/>
            <dbReference type="ChEBI" id="CHEBI:15441"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="1.14.14.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Microsome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the squalene monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.17" evidence="13"/>
    <dbReference type="EMBL" id="AJWJ01000309">
        <property type="protein sequence ID" value="KAF2072119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5U2"/>
    <dbReference type="OrthoDB" id="1678617at2759"/>
    <dbReference type="UniPathway" id="UPA00767">
        <property type="reaction ID" value="UER00752"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004506">
        <property type="term" value="F:squalene monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000166">
        <property type="entry name" value="Squalene monooxygenase Erg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013698">
        <property type="entry name" value="Squalene_epoxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040125">
        <property type="entry name" value="Squalene_monox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10835">
        <property type="entry name" value="SQUALENE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10835:SF0">
        <property type="entry name" value="SQUALENE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08491">
        <property type="entry name" value="SE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 13" id="KW-0274">FAD</keyword>
    <keyword evidence="5 13" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Squalene epoxidase" evidence="14">
        <location>
            <begin position="186"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2072119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="490" mass="54144" checksum="9E1550E54A54736D" modified="2022-05-25" version="1">MEPINSSLFIEDQKFDIIIVGAGVAGAAFAYSLGSKGKKVLCLERDLSEPDRIVGELMQPGGVKQLRELGMEDCFDGIDSALVFGYGIFKESKGVKLQYPKDKNGSIINGYSFHHGRFVQKLRYKASTCQNVTLVEGTVKSLIEEDGVVYGVRFIENNKIIEISNQDNQNNQDNQDNNSNIKEVRAPLTLVCDGCFSNLRKNVINDPTPLFTSTFVGLVIRGCQLPFQSHGHVFLCNPAPILMYRIGSDEIRVLIDIPGKCPTNNDLKEFIKTVTVPQLPESLREALLTALDTDQLKKMPNSRLHPQTISKPGVIMIGDAWNMRHPLTGSGMTVALSDVNILSRHIGSISDLSDRKQISKTLQMFMVERKPMASTLNVLAGALYQVFSASNEHLRQACLGYLDLGGEFSAGPVSLLSGLRPKPRVLAMHFFAVAFYGVLKNLFPFPTPSKIIRSYRILCAASDIVVPLLRSEGVLKYLTFLCVALRLTNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V710</accession>
    <name>A0A8J4V710_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RGS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002339</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000063">
        <property type="protein sequence ID" value="KAF2076382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V710"/>
    <dbReference type="OrthoDB" id="196547at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07440">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845">
        <property type="entry name" value="REGULATOR OF G PROTEIN SIGNALING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845:SF270">
        <property type="entry name" value="RGS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01301">
        <property type="entry name" value="RGSPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RGS" evidence="1">
        <location>
            <begin position="3"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="153" mass="18525" checksum="93CE6446A4DFB0EB" modified="2022-05-25" version="1">MVGLDQIVQDRNLALMFRKFLYERFNNENFSFWLEAENYKYLSTPTQRCSRSKEMYEKYFSSNSKYELNIDHFAKKDLEDKINTGNPNIDVFNQIQNTIRKLMEMDVIPLFIKSEYYKKYLESKTIDIDISSERDRSVTLVMMEEFFSHKQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V812</accession>
    <name>A0A8J4V812_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Lysosomal dipeptide transporter MFSD1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Major facilitator superfamily domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">CYY_001360</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Lysosomal dipeptide uniporter that selectively exports lysine, arginine or histidine-containing dipeptides with a net positive charge from the lysosome lumen into the cytosol. Could play a role in a specific type of protein O-glycosylation indirectly regulating macrophages migration and tissue invasion. Also essential for liver homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-alanyl-L-lysine(out) = L-alanyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79415"/>
            <dbReference type="ChEBI" id="CHEBI:192470"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-alpha-aminoacyl-L-arginine(out) = L-alpha-aminoacyl-L-arginine(in)</text>
            <dbReference type="Rhea" id="RHEA:79367"/>
            <dbReference type="ChEBI" id="CHEBI:229968"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-alpha-aminoacyl-L-histidine(out) = L-alpha-aminoacyl-L-histidine(in)</text>
            <dbReference type="Rhea" id="RHEA:79375"/>
            <dbReference type="ChEBI" id="CHEBI:229967"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-alpha-aminoacyl-L-lysine(out) = L-alpha-aminoacyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79383"/>
            <dbReference type="ChEBI" id="CHEBI:229966"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-arginyl-L-alpha-amino acid(out) = L-arginyl-L-alpha-amino acid(in)</text>
            <dbReference type="Rhea" id="RHEA:79371"/>
            <dbReference type="ChEBI" id="CHEBI:84315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-arginyl-glycine(out) = L-arginyl-glycine(in)</text>
            <dbReference type="Rhea" id="RHEA:79391"/>
            <dbReference type="ChEBI" id="CHEBI:229955"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-aspartyl-L-lysine(out) = L-aspartyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79411"/>
            <dbReference type="ChEBI" id="CHEBI:229953"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-histidyl-L-alpha-amino acid(out) = L-histidyl-L-alpha-amino acid(in)</text>
            <dbReference type="Rhea" id="RHEA:79379"/>
            <dbReference type="ChEBI" id="CHEBI:229964"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>L-histidyl-glycine(out) = L-histidyl-glycine(in)</text>
            <dbReference type="Rhea" id="RHEA:79395"/>
            <dbReference type="ChEBI" id="CHEBI:229957"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-lysyl-L-alanine(out) = L-lysyl-L-alanine(in)</text>
            <dbReference type="Rhea" id="RHEA:79399"/>
            <dbReference type="ChEBI" id="CHEBI:229954"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-lysyl-L-alpha-amino acid(out) = L-lysyl-L-alpha-amino acid(in)</text>
            <dbReference type="Rhea" id="RHEA:79387"/>
            <dbReference type="ChEBI" id="CHEBI:229965"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-lysyl-L-lysine(out) = L-lysyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79403"/>
            <dbReference type="ChEBI" id="CHEBI:229956"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-lysyl-glycine(out) = L-lysyl-glycine(in)</text>
            <dbReference type="Rhea" id="RHEA:79407"/>
            <dbReference type="ChEBI" id="CHEBI:191202"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="18">Homodimer. Interacts with lysosomal protein GLMP (via lumenal domain); the interaction starts while both proteins are still in the endoplasmic reticulum and is required for stabilization of MFSD1 in lysosomes but has no direct effect on its targeting to lysosomes or transporter activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000031">
        <property type="protein sequence ID" value="KAF2077357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V812"/>
    <dbReference type="OrthoDB" id="424834at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052187">
        <property type="entry name" value="MFSD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512:SF7">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="20" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="361"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="398"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="451"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="486"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="517"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="21">
        <location>
            <begin position="85"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00044876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00044878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00045018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00045709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00046376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAF2077357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="574" mass="63520" checksum="53E34552F70041D6" modified="2022-05-25" version="1">MDYRDDYSSSTSTEDDKGADIILSSMTVLDSKDDMKESLVANIDTLQEQQDTHNNDTSTPTEFKHNIKSTNKIQSILNFLKNEKKKIIVTFLIINLGFPLYLSYNNPTSMVKVFNNYFNLSPTQFGNLYTVYALPNLFMVFLGGIVIDMYGSNRCSIIFSGIMTLSAIFAALSTTPPTYSVLLLSRILLGIGGESLLVCVNCFITEWYPAKEISLIIGLESAWVQFGSLFAFGVLPSMYAMLGSLPMTEWVVALIAIISFILNLLFIIFSKKLVFAGSPKLDQHKLLKDQETGDMTGAGSPTLGGDNLLEVDLDEEDYIDGADHQDQIHQQQQQQQQMKIVIVFQQLKQAFKLVRLISSRMWLVIVIAFFGYSSFFGFDIICTDMVIEKYGYSDTTAALMMAAETLCTGACSPVFGWINKKLRRKILSMGIGAFVMGFGIVLVIITPASVVPMPFIIISGLGYGLFSNAVYSSVPTLVDANVTGTSFGLISTSYNAGIVLFPLFLSAFKQATGNYNLSMWTLVLSAFITIALLVFLKKLDEKEKDTDKKMDAIPIMDLFKFINKSSSSSSTPTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8L3</accession>
    <name>A0A8J4V8L3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003527</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000111">
        <property type="protein sequence ID" value="KAF2075179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8L3"/>
    <dbReference type="OrthoDB" id="19499at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021837">
        <property type="entry name" value="CfaA/B/C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33576">
        <property type="entry name" value="CARBOHYDRATE BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33576:SF9">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11912">
        <property type="entry name" value="CfaA_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035261214" evidence="1">
        <location>
            <begin position="17"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="218" mass="24933" checksum="2513A17526225783" modified="2022-05-25" version="1">MKGFLFICLLFTICNCIDYSYFYPPQKNEAISGVGYIVGLNQGCVSYDAQGWSAEAVAENDGQSVNLVLYEQHDCSGSPQFNQTMDAQNNIYTIPFNKYDPNNRFMVGVYDPPFQVETVMVMQFYPENNQCVGQAPILKMFFNGTYFNSTDPIAQEYCDNDVPMMKRCESAHCQNSPYPSNCTQYFSNYIWSNFFCRIGVPTDSSSLFDYNNEVLIHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VAE7</accession>
    <name>A0A8J4VAE7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_001621</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000040">
        <property type="protein sequence ID" value="KAF2077054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10017054at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="5">
        <location>
            <begin position="15"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="5">
        <location>
            <begin position="139"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="309"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="784"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="834"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="337"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="419"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="715"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="1080"/>
            <end position="1222"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="1262"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="784"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="834"/>
            <end position="848"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2077054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1406" mass="162709" checksum="D177EED01F6CF9E3" modified="2022-05-25" version="1">MNKENNNNKDKDWVGGQQKVFTNWCNIYLGKRELSIQDLKTDLYDGILLGNLIEVLSGNNNILSKCKKMNSRVHHINNLNYSLDAIKSEGINVSCGSEDITDGNLKLIMGLIWILILRYQIQMPKQNLNSSTNGTTSGRSPSEMMLNWVKSELEDYNLPINDFTKSFSNGLAFCALVHRVVPQHIDYENLKAENSLYNLQLAFDTAKNILNVPAILDPSDIIDELCVQTYISLFPKVYNNIREQQQQQQQQQLDSNSLVSPNKRSSIQLTKSFIDQQIQSQQQQQQRVLLSPSRSSNLLSSIDISVSDISSNSGLSRSESNTSVNSIGSSRSNSSNNLLIEKKIQELQLQLSDKEKEIQMLTSEIRDKVSENVELQHKAMDFEELVRLINNLTSEISENIEQQEASEPRKQFEEIVKETKRKGEEINRLNQTIQAIKEEKKSCDCAPQILLFETKVKELETTVVDKEKGIEKLNTDLQSLEQERQDLVNKLDSLTTQENEKIVAIQDLENKYNELVAKSKETETSLLQSDGDLSRERENFIKEKETLIKEKETLVKEKELLSNETVELAYQIQLLTKEKETLVHEKAESTLKIDQLLQEISALKQENDQLLLNNKSQDEQLLTKLKEKQDNIESLNNEKQKLLDQVRELEEIQNKVEEKEKMIVDSNQEKQDLLDQLSQYKINVQEQLESIGTLNKDKQDLSVQLATLQDMDSALKSKDQEISDLHSDKEDLLNQMENFKILKNNLNNEYESLVKEMEALKNNQHSLSSDQGKEIQELKDKLDQREKDGLEKDNLVSELNGKLSDREAQSNSLNSTLQERDTELNELKIKIQQLKDQQQQQSSNNQDQAEMEKNRQLLEQSENEIKQLKEKMDEKQREYSSKIEEKDNELKELNSRIQELTNKLQELQNRLDEKDNELKELRDRVDELLNQLESSRNENKQLNIQIQEITIVINNITIEKQNLQSQLDNYKQLESKVDDKQQFIDQIQLEKDQLLEQIQGYKSIESQLDEKESILKQIQMERDQLSKDLEQLKLDQEKTILEQSNEFENMKSTKDKEIESLLSTHSNHIKEIQQQHQIDIDNLKQINQTLSSNSDQKEELEKQITISNELRALLEKNQATIDQLQNENQALKEKQDSIDTTQAQEYKQLESTVNSLNEDLNQYKLEKDSFSNQIQSLQQENEQLAKQNREKESLFSDKESELTRLLSQIDEKDLLIQRLNQQLLQVNNSNIEISSFADKYETVVKEFKEYRLTTETKLQSILAEKQSESADLKSTHQALQDRIDKLIKEHESNSTLYEKTKKDYETKLLAAKSQLQSDLDSHEALTNNLSDQIKKLSEKNSKYKEKIKEQQSQINTLQTTSISTTTNTNTSSAFCKYNGILLFASVLVYAALKIFGPTESNFEKPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VB55</accession>
    <name>A0A8J4VB55_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_000811</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000017">
        <property type="protein sequence ID" value="KAF2077849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VB55"/>
    <dbReference type="OrthoDB" id="9974421at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000057">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006693">
        <property type="entry name" value="AB_hydrolase_lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025483">
        <property type="entry name" value="Lipase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04083">
        <property type="entry name" value="Abhydro_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000862">
        <property type="entry name" value="Steryl_ester_lip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035253815" description="Lipase" evidence="9">
        <location>
            <begin position="21"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Partial AB-hydrolase lipase" evidence="10">
        <location>
            <begin position="31"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="336"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000862-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2077849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="392" mass="44028" checksum="41DE2C49880A8CE1" modified="2022-05-25" version="1">MNNIIKTILLIVVSIQLVNCLTVSQEASSSIEEIVASYGYPIEKHSVLTTDGYILTVHRIPFGKNGKTSNNKPILLQHGLCDSSFTWIFNLPGYGLGYILADQGYDVWMINFRGNAYSLGHTKFTDKDSAFWDFSFDQLGLYDTPNTIDYILRQTGATKCAIVGHSQGNQAAWISFLRVAGFSNKVSYVAALAPISTITNMRNDFFNVMAELHVDGALVGLGFQRFLPKPSTLRKEFILYCGLCKRCCATGIEMLVGPHNGPFNDTRNPVISGHEPAGTSLKNMQHFSQIKRNKKFQMFDYGKDNQKMYKQSSPPVYDLTKLPNNLNLAFFYGKEDVLSTTTDVQILINQLPKGVLKHSQLVDNYAHIDFVWSLDANTLIYSKIIEQLKVYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VBY2</accession>
    <name>A0A8J4VBY2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000001">
        <property type="protein sequence ID" value="KAF2078659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VBY2"/>
    <dbReference type="OrthoDB" id="17512at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.340">
        <property type="entry name" value="Rel homology domain (RHD), DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037059">
        <property type="entry name" value="RHD_DNA_bind_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="714" mass="80176" checksum="E0F049784C78BA2C" modified="2022-05-25" version="1">MDDLLQQLQPLLLINQLNQHHEESLEKQKAIFNPSTVAVSSKSDKVIDDFYKEENDYYFKKSKELKTLCNNFIHKQNHLILQGLELQSNSINNNSNSSLSTSSPIGSEPSFNQLRIRSSSSGSAQNTTLLSSSAAILSSSNSSLSCSSSSSLSSSISSGGNPLARSLSISFEDFPLLMLEQQRYMIQYSNLLIQELSQSSSIINIHKEHIDQLVRDCGFVVSTMLHEKLAELHNLSKHISILQKELEKIVIYFSSEEGSRNDNNNATCKGQLDLLSNSAILIFQKQPFPGIIKRFKQIHSDEFILELLTTSHFPYTYGKVKVHSNSPISSLSSSGSLNNPSIDSPTNSNTINTSTTTTTTTTTTTTSTTSTTTSTTSLSSSSSNIPIPSGSSSLSSSSSSSGSNIGASASTTNANSTSSSSTQINANSKSDLFEQQTFDLISAPEGNRMIAKIPIKFTKGTTQFSTLKFTLNLIHKETKEQFTIETENSEKFVVFTNDSQWVDYYCLLLKEEVFLYQADSQQYLFQQVLHPRVIQIVQMYFHLCLKIYKKHPSHYPNLSAEPLKDLLNQISDDNMITLDQFDSFISVLSPVFKVLKYQKHAMDLWNSGLIFLFIKDEKIQEYLNGKPGKFLIQFSLNHPYTWVIKYFDEKESMIKEILVDMKLDLTGDRANKSLVTYIENNLQSFKEICQFDQKHNQIHFLNKNEALTQIKQKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJC0</accession>
    <name>A0A8J4PJC0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MACPF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010307</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001030">
        <property type="protein sequence ID" value="KAF2068367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJC0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MACPF" evidence="2">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="567" mass="64785" checksum="BD8DE672BBB72533" modified="2022-05-25" version="1" fragment="single">KSTNVRTENKDEWTESVSGSFSASVSSPTFSVSGSVSAAVDKSQSEQQQQEKQESSTTSRMMVYGGIPAAFSPAEDGQSSPGFKEWASSIDVLPVPVDYQLYPIKMLLNSLWVNKHGVNIRALWEEAEISYYKGEAFSEEPNPNNPLVSFSLIYEFNVDSPILKQNYWGSNLSPILDITYYVVESGAEVSKNLKIPIEFQYTNCKGEMFVSDFVQDNTPVGKKEGEITAFYMNTPNPAGTQYKTFEPHPTKSLKYPLKFDFINVDFFLSTKKPIIKITLPTGFPPLTNSPSKPSKIISWKTYKAILFNENGVIVAPISGTRNYYATAAFEHYWSYNATSRNVKYPSFGFPNSNEVPFICYPANTADQCTDGIEFQYRGPSVPISETDPRPKPILPKAFSTAFNRDYNVPSPSRTWFSFSNPTADPLYPKNLFFVENINKLGLNARVNWLKIYYPNIDDPWRFDILTYHINNEKSIYDSTNYINTDFAENTDLSYKFFKLESFGPTNLVARQLYFYHNYNYNGGIIDSYEYFYQNAPYYSSIQNKNSNFDVFEDLEERTSHRFFEKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJG0</accession>
    <name>A0A8J4PJG0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CDP-alcohol phosphatidyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_010201</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000971">
        <property type="protein sequence ID" value="KAF2068472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJG0"/>
    <dbReference type="OrthoDB" id="196717at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014472">
        <property type="entry name" value="CHOPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414:SF75">
        <property type="entry name" value="CDP-ALCOHOL PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414">
        <property type="entry name" value="ETHANOLAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015665">
        <property type="entry name" value="CHOPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="222"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="250"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2068472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="354" mass="39784" checksum="E1777C8E61A7145B" modified="2022-05-25" version="1">MVSITLTGLFCNIAMYLIMYFYCPTLTEAAPRWTYFAVAFLIFAYQTLDNVDGKQARKTKSSSPLGELFDHCCDAVSVAMFAAVMSSTLRIGSSWAFLCVLVGIWPFYLAHWEEYHAGILVLGEFNGPTEAQVLFIIIEIITGIFGSDIWTYGRFAVGNLAAIAVSVGALATMYQNFRNTYALPNRIQFSKCLLQLLPIVTFSILIIVWVSVTELIHSKPHLFIMTIGMIFSYIQSRYITQRVCHDDCHLTYPIFIPLAIVVFNSILAKSGHAIIKEEIALWILFSISIVQFLLFAYFTTIQLCNHLKIKVFTIPYSSGGSSNQEYSNSLLSHMEEGDIKDNDPNSHNSDEAMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKW8</accession>
    <name>A0A8J4PKW8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009773</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000802">
        <property type="protein sequence ID" value="KAF2068902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKW8"/>
    <dbReference type="OrthoDB" id="1935586at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="402" mass="45469" checksum="2FA4BA22E3912524" modified="2022-05-25" version="1">MTVAHRPQADGQTERVNQEIKRILIKFVSEYGNDWANIIGLVEFSINSAYNKTTKFSPFQIVYGFNPATPVNHFSPKMGKINIDTIKALVRDNILDSQVEAQKYYNKGRATNTLKEGDKVFVKRSDFADIKDVVAKKLLTKNAGPYIIQEIRGNNAVLDLSNTIHERKNHTLNIDQLIKIKEDIDFPRVGISMPNPISDDNRYEIEKVVNYDKDKNKLQVKFKGYGDIEWVEIDEQNKYLVDKFIKEKGEESILNSRKRKQEDAKIKANNKKQKVTINKPSIVVVPQNDSKHKVTKTVINKPTVATQPEHKVANKIVKQDTNSKTNQPATKSVVVVNKTVASQNVSSTANTNNPKNVIVNRSANVSVAPRKNYSNIKFNNKYMAIGKPTTKGMLVPLPSNHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM31</accession>
    <name>A0A8J4PM31_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_009120</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000639">
        <property type="protein sequence ID" value="KAF2069564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM31"/>
    <dbReference type="OrthoDB" id="17983at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006838">
        <property type="entry name" value="ADTRP_AIG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10989">
        <property type="entry name" value="ANDROGEN-INDUCED PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10989:SF16">
        <property type="entry name" value="AT02829P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04750">
        <property type="entry name" value="Far-17a_AIG1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="88"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="194"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2069564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="267" mass="31615" checksum="BCDCDB23593B8C01" modified="2022-05-25" version="1">MKFLLFIIHSFFFYIEIYGILEYIKVFNTMGVYGHYDIITLLTFLTFWSQLILAFFYSVSILVDLIDLTKLFDKTSDSYYSIVKQRDLFFRAIVFPIGFSLTVIFWTLYAYDRELIFPSFLDTFFPKYLNLIQHALPGFLVLFEILIVNHQFHLQRNLIENNNTYKKNLKAKISSSSIEYTPITSNVKQDKTNFLFFVFFYLSILVYTRCHKGFWLYPILEVIPMHFKILFIFIACGFGLLAYKVGGDINQSRWGGRFVFTISDSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMC5</accession>
    <name>A0A8J4PMC5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000618">
        <property type="protein sequence ID" value="KAF2069668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMC5"/>
    <dbReference type="OrthoDB" id="444581at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF173">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="565" mass="64011" checksum="D32B913BE199D76F" modified="2022-05-25" version="1">MISNILSYLFKENANINKNNHTINNPCDLNRNSSVISNSSSSSSSSKRYSGNSLKNELFFTIWKNQFLRFSVFLNFGNYDLQVIFQKVSDFEERVEQSCMFTKFKSIHLTICCDLKDIDNLITIYNKHKSTLKQRITFFQLNVYARATVVLCIPDFVDKVSFSNSYTGNIYANELPNSLKELEIGERYSGVITSLPDSIKKLSLGLSSQCLMTKNFFPQNLKVLKFNCSVSHPLKKGMFPDTIEYLYINFGYAKGENPLTSECLPKRLLYLNVLNYKYKFPSDCIPKSLEKFSIGPNYELEISDQLPSTLKSLKISVLDDDLKRFKCLETLSIGSIRKFSGFSPFSSCSLPSTITNLSIIGEFNCVIPSSVTQLEIGEFANIPSTIPKSVKKLKIGGPNHSFSSLPSGITHLTFNDSFNESLSHIKLPDTLLHLDLGCHFNYPIGKNDLPESLTSLTFSTRFNEPLTAGVIPSNVQTLKFGDLFNQPLVENLIPPSVCHLKFGYRFCQNLFDYLLPPRLTLLSLDSSYIRDFDLPKTLSHLQIYNRLWSFTPDSSLLSLQKLITN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMJ4</accession>
    <name>A0A8J4PMJ4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_009101</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000637">
        <property type="protein sequence ID" value="KAF2069575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMJ4"/>
    <dbReference type="OrthoDB" id="20727at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173">
        <property type="entry name" value="ANKYRIN REPEAT CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173:SF74">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="183"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="259"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="401"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="469"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="412"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="474"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="496"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2069575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="545" mass="62988" checksum="60BD25A9ED697A15" modified="2022-05-25" version="1" fragment="single">MDSFHTFLFQCYRHFDNFVGRYLQVSKSLKLDFLEIGHFILNAVEEKQILINGRNQDKGLLLNQYTEELSKFVDSFEIHVLLEYAMCLCFFSANDELSLELLRSNHNLFVRYLIGKDFKEGVGFCFAVPYASRVLLQYLVSIGMDLTMTIDNSFIMTSTLYGNIQAFDFFVEKALKLNHRNSNGDTAILFAAMNGDTKMIEKLIMCGEDLLVVNNHGFNVFICAAFHNQHEAVRVLAKHIQEKYGQKKKKEMIDHKDKDGETPLHIGARLGFASTVKALLEQDAEMNIVNKTLHTPLDMAKKAIAAMKKDPQYLSNQNNLQIQKKLTHFRTELKNLVYVQQLLPQDFRDNVLFSKPFFENQICSTDPFNQVISLFKEHFEIREALISKQLEDLLINEKKSKSLSAKKKPKKNNSNNSNNNNSNSDGRKKTSTTTQIIQQVSEKINDQSNIEKINENLLNINNDKLKINNDKLKSNYNNNEKANKNSNNNKKSNSKTKNESNDKDKQSKNNIPEIKKEIKVEQKKEQEKESEKEIKVEQKKEQERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN12</accession>
    <name>A0A8J4PN12_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008447</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000521">
        <property type="protein sequence ID" value="KAF2070239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN12"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="147" mass="16561" checksum="793BDB00037ED837" modified="2022-05-25" version="1">MQPLCGELLNEALEKAALGGHLSVIKYLTSTYPSLLAQRGQPQPRLTVPLKVLLDAVIYNHFPVVKYLVPLFFPDPILPPTTLKSLGSSGNLMMVQFFYKRLKLTDFRPTLSNAVRDGYPDIVDYLHDKGIYQLEEILLMETTAQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN90</accession>
    <name>A0A8J4PN90_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008223</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000489">
        <property type="protein sequence ID" value="KAF2070458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN90"/>
    <dbReference type="OrthoDB" id="16938at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="135" mass="15394" checksum="04E213CD333A3F05" modified="2022-05-25" version="1">MLDNRLAFNIKRNVILKDKNGNISEIDIVYGFIFKKYIECKCYTSQPVPLKDVAKFKEVLLMNNISPHQGLFFTTSTYVPRASTIGILTIDGEQLKSMERTSFFVGIFKSFAYVFGTALGLGLISVFIKEEYKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNM1</accession>
    <name>A0A8J4PNM1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008920</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000599">
        <property type="protein sequence ID" value="KAF2069756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNM1"/>
    <dbReference type="OrthoDB" id="659430at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="34"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1040" mass="118839" checksum="BC192C59D672EA20" modified="2022-05-25" version="1" fragment="single">ENNHFTNNWLCRKGYLPPSITHLKINFYFERDAKDNNNDDGEQDDNNNKNNNKNNNNIDDHDEHDDNDDVEIETKEEKMEIERNKDKELNNDQDNEIEIDSNSTHPLKKRKIDQNDDGDQDPLVSKQDEFHEDEEGYEPLPSSLKILIPSSIQSVCFCLDYKSYRSHVTVLDKDDYYSTAPTPPKIRQGLKELVLPERFNNRIHKNTLPSTLQSLSINNLDYNNPLNSNNLPLSLTSFKCNSDEIKLDLSEFESIKELKVYADAITYYPPNVEALKLISPSKKGYQFKLNKHLIKSLVYSCNTRDQFRFSNLKELLDTLPNIQYLSSEFLDFEVPASVQELKCLHKSFRCTDARPISSPAFETLHLDLGYYLENHSQTQDNHQRGEKIINNYKPNQQPMLNTFFKIWRNIYLKPLVLEALYDLTKSPLRFDSKTGTLEHIDRFKQYNILVKDVTQLGPALLKFDKADEISFYGEENGTTNNNLSSFISKSIKKLRCSFTKGIPDWITHLSLENISLCDIQVDDIPPSVTNLTIDNNDEAYIGKIPPSVRYLTLNKLMHNLAHCIPPSVTYLYLANTAFNDINSTDIPSTVQRIELKNLMYQKDKGEYDIPSFISSRLVNGVFYIYSKSKNRITIPSNTTHLFWLDNQYIDNDGDGINIPPSVHTLVLDETFNSAILTLPPTITQMIFQGDFRQSFACISFPPALKYLSLGELLGFMQEEDLPKGLTHLSLGDTRGVKSLPQSVHHLESNSMQNYDRIPPQVKYLKFQSRYKIFLPTTIKSFKSPYGKITLEEPTDSPLVKKSSLVSSFYSDSTSIHLYSCKHFNSFIVPHTLGNNIKSISFGDSFNQVLLKGTLPNCVEKLVFGDVFNHKLNKSILPTSLLTLVLGNDFNQNLNDNLPSSLTELMLNTGSKPSFTSSSQFPPNLKKLALPYYDGVLDIIPTTINHLEFNRSITQQKYIIFPIELVPPHIKTLVLHDSMRIQSYHLIPASITSITLCDSITPYTKIPCTVESVVLPSSFNQPLDTILQTVDDHDDKDDGDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNM7</accession>
    <name>A0A8J4PNM7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_008389</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000512">
        <property type="protein sequence ID" value="KAF2070297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNM7"/>
    <dbReference type="OrthoDB" id="417037at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="6">
        <location>
            <begin position="22"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2070297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="314" mass="34752" checksum="FA8C6E8812B09310" modified="2022-05-25" version="1">MESMLKFISENKGFFVAVGYGVTSVSITFFNKAVLNYYNFNYSNALTLGQMIFSLFFLVLMKQMGKISYPDVNVDLCKKLFSLSLLFILMVVSGLAALAKTNVPLFSALRRLSTLVVILGEKMLLGKVTPADETQAVVVMVLGALVAGWGDVTFDFVGSIYILFNCVVTAGYLIYIAKKTKETGLNTFGLMFYCNILSMPATIVLTFITEWEGISTFTGYADLGFQFCFFMSSIQAFLLNYFIFLCSTLNSPLTTSITGQIKSILQTIIGLFMFGDVILSPLLIFGLVVSTLASFWYSFIKYAQTAARKITQSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNN5</accession>
    <name>A0A8J4PNN5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PA14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_009274</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000668">
        <property type="protein sequence ID" value="KAF2069409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNN5"/>
    <dbReference type="OrthoDB" id="21110at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022829">
        <property type="term" value="F:wide pore channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011658">
        <property type="entry name" value="PA14_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052784">
        <property type="entry name" value="Perforin-1_pore-forming"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46096">
        <property type="entry name" value="PERFORIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46096:SF3">
        <property type="entry name" value="PERFORIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07691">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PA14" evidence="4">
        <location>
            <begin position="257"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="MACPF" evidence="3">
        <location>
            <begin position="462"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="710"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1062"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2069409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1062" mass="119607" checksum="2C511C9E93222178" modified="2022-05-25" version="1" fragment="single">DAINEIRKTNYPKDTAVYLYVKEGDYFGVNNKGLDIDFNLNIISTKGSSKTIIDCQKSGYAFRFSGSVDTSISINGFTIRRCASSKGAAIHVSNYDATIEDLVFFENRAREGSAIYVADSTRLLMIDDCAFHHNTGSAALYLTNIENVDIRSTDFNSNDNDVVCGGNTFITSADGSFFGSTCNDQCQIVDEIEYDDLCGQTRFFSPVNCDFDGQCQSTENSQSCPSDCPFNQFPCNNNGLCDSNENFDTCPKDCSRQDHPGWKLDVYDVLLDKPMLSSASGDNRDSNLYSSEFLAFSEFTNFKSRMPVFSARMTTYVTVVQGGYYFFKLETNNIDAIIFVNGRVLFDTYRQGIQEKFVNQRKLLLSQGDKTFIEIVFYATSTIQRDLSLEWRNEKEQLYKSITGQYSLTKDQFYCGDGVCNEVHPESCLIDCYSLIEEICPGQSPPAKLQDYYQNTNQDLVGTLLNNQYILSLPGINYMSHGIDIRTGVPNASPIFDLTYCDNTSFSIVQNPYRGLVYSVPQGLFAQISPKCTTDSKTKTFSTARQMAQEEAIEKEFGFQVPPILLWKVLVSGAYSESESTNTASEMEKRNSGTVSKSTIMCESTKVHLVKEKLHPKFIADIAKTFVKDLQGVYNQDASDENLKQLIIKYGSLYHRSAILGGKLEIISTVKSSYSRKKTENEISNARDVTVNAQASGGILTGTATVTDSLDSTTKEVDQSEYESNSERSSVKVYGGKSGSYGLDEPNAFASWAQTVDLVPYPIDYKVGMVADVIPEYWFFQGEYNVKEAWERCEMNLYSEYFKSRQYKYIDFGVKVGRDQTMYNVQGIVIAVEFKLTIVDSKGVSHVFENVPAFDSKNNMILSYNDNNGIRSLKFEHSSSTIPLPDNIEIYNIFSSRFYSLKKDVGSNLMVTQSSTPDLLVLDFKAVHYEALQDDLHCDIQIKIIGTLNHYTETQPISIFSRYVPQDYTWTILADNFLGKIIGIEFSFFRQSDSSRFSVFFDSLLLRQSCPIEEISSCLPLGTVFDEIGYTKAYERWRDQTILTNNQQETDPVYISIELLGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP62</accession>
    <name>A0A8J4PP62_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane protein 19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_007782</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000434">
        <property type="protein sequence ID" value="KAF2070899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP62"/>
    <dbReference type="OrthoDB" id="30881at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002794">
        <property type="entry name" value="DUF92_TMEM19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13353">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13353:SF5">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01940">
        <property type="entry name" value="DUF92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2070899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="33593" checksum="56BD9C1B688FC0C6" modified="2022-05-25" version="1">MDYIIDTTTKEGAAIGRLPLAFLIVSFFGIHGYRKGSLSLSGLISAWLVGMISCYSSWTIAVTLLTFYYSSSKLTKYKAKIKKALEDNHTEGGNRNYIQVFSNSLSATIYAIMFFLYTNRLSTIINYNYDYFGSFLLCAIIGHYACCNGDTWASELGILSKSQPILITTLKRVPKGTNGGISKTGILASLSGGLLIGVFYYLATITFSTDNDEYRSLSSTFHLLPILLLSTLSGLIGSLIDSILGATLQASLWSENKKVIVSEERKIPSSERTKLISGKKILNNHQVNFISSLATSLLAGLLGQLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPA5</accession>
    <name>A0A8J4PPA5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7743 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008153</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000479">
        <property type="protein sequence ID" value="KAF2070535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPA5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035258177" description="DUF7743 domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="2">
        <location>
            <begin position="436"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="588" mass="65902" checksum="196F5B546516EE35" modified="2022-05-25" version="1" fragment="single">MWKLCIFIFCTIQLCLGLPSLSQVPTNQVLKSYMESSCAQKYLLHLTDVPEVDSPNFRELTNGNVMISVGNVFSKGPGDIYFTLDILQITSEGTYPINLSLEYPNGTFYDFGPVLEQECVSPPSTLTIKPFDDKLKIPRVYPENNMMVYSIVIENIDRALNIAPYEIDCGLGVYYCEIYGSSVWNQFDIFVDLKAAIVPKDVVTPITATLKGNVAYQFTIPPPLNSSFVTPTTVQTQTLTAAMAKYLPNYYSINTESAPLQEFSFIGFSSSARSTLSDLNSMVFYQDSTKKCSLHWNHLFRDRGLNFQGFSINNNQLVSSFPDPIEYVAYDSPKNYLSNTNAQLSLITTYNIFTIELYNLYNPKMNYVIGQGFGPYSEFKNNKYPMGFLGQNGKRFTFRFSFLVSKYYKGESGNLFYAGFGGLTSSTLSGQADSLTPDVIAPVIVDLKYAPLENGMIHIVCKVTDNLSGVVNIYFYDLSDNVITFMTLQNLVSGVSTDGSFEIVVNPLIYSTVTRVMIQDAAGNMDIFYSSDIYTLPNIPGWFGYAKLLDQIQPLFNDQYIGVSNINQITNLQFSENNLNLLQPKTIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ61</accession>
    <name>A0A8J4PQ61_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006710</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000321">
        <property type="protein sequence ID" value="KAF2071973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ61"/>
    <dbReference type="OrthoDB" id="19802at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05379">
        <property type="entry name" value="CAP_bacterial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31157">
        <property type="entry name" value="SCP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31157:SF1">
        <property type="entry name" value="SCP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035172770" description="SCP domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="2">
        <location>
            <begin position="31"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="160" mass="17350" checksum="C2AC49649D589B36" modified="2022-05-25" version="1">MYTIKSTCLTIFVILISIVVINAQTNPSLHLSLVNAERARVGLKPLLLSPCLMASAQVQSNYQSSINTMTHSSPLGGLFERTRPFGVACNSVAENVAYGYKDDASVMKGWMNSSGHRANILGQFTLFGVAVSKDNNGDLYWTQQFARGTCNRNNSTAPAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQK8</accession>
    <name>A0A8J4PQK8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_006335</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000288">
        <property type="protein sequence ID" value="KAF2072340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQK8"/>
    <dbReference type="OrthoDB" id="21539at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034063">
        <property type="term" value="P:stress granule assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025762">
        <property type="entry name" value="DFDF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019050">
        <property type="entry name" value="FDF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025761">
        <property type="entry name" value="FFD_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025609">
        <property type="entry name" value="Lsm14-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025768">
        <property type="entry name" value="TFG_box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13586">
        <property type="entry name" value="SCD6 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13586:SF0">
        <property type="entry name" value="TRAILER HITCH, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12701">
        <property type="entry name" value="LSM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01199">
        <property type="entry name" value="FDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51512">
        <property type="entry name" value="DFDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51513">
        <property type="entry name" value="FFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51536">
        <property type="entry name" value="TFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DFDF" evidence="4">
        <location>
            <begin position="424"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="FFD box profile" evidence="5">
        <location>
            <begin position="506"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="TFG box profile" evidence="6">
        <location>
            <begin position="531"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="36"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="188"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="327"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="447"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="547"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="FFD box" evidence="1">
        <location>
            <begin position="506"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="TFG box" evidence="2">
        <location>
            <begin position="531"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="39"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="133"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="220"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="327"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="400"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="455"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="566"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2072340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="593" mass="63612" checksum="096FB1455915D14A" modified="2022-05-25" version="1" fragment="single">RAFGTEGRRQGNQIPPSAEIFDYIVFKSTDISDLNICEPAANTPSTTGATPAASSTSSTLPTSTPSSTSATTVSKPTSSATPSTPPVAGATTPSASAPSSLPNSGGNASTPSSASSTPSAGRMPSMPTGFPSSQPPQPNPQQPNPQQPYGQQMYSNYQFMPPGYSLPHMYNPYFNYYGYPYMQNQGMPPQGMPPQGMPPQGMPQGMPPQGMPNSPFYKMPQQVQPPQAVPVTPLSSSNPAATPAAATSTATPSSSSSSTPSTTSSTTNNNASPSLNSAPSTNAPAAAPTTTPSVVQPVISTPTPVAPKPTTAAAVVAAAAGITPTIVQESKPQQQQQQQQQDRQPKVYNNNQQQQQQNKPRQYNNNRQQNNQQVNQQQNNQQQQQQPNQWRKNTHNKNQNGTTSPTNVTNNTQQNRTGGYKKNYPQKQTAKDLEEFDFDEANSRFSKEKVAQELTSSSSSTTTTTTTTTEESSNINNSSSNNNGTEAEDVEIISNGVSSITVSTIQAYTPSSFFDNISCEALDKQSEHPAPKNKSLYEQRKLDQETFGFSSVRYGNNNRGRGRGNGRPNTSSRYYNNNNNRNSYQQKQASKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQN9</accession>
    <name>A0A8J4PQN9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008257</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000493">
        <property type="protein sequence ID" value="KAF2070429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQN9"/>
    <dbReference type="OrthoDB" id="48988at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="1">
        <location>
            <begin position="73"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="506" mass="57969" checksum="2770E256AA774758" modified="2022-05-25" version="1">MIKNIFTINNTLKLFSRHKNGFIYFSSSSMSLNGATSLSTENYFKKLNNNNNSCSSPVKLPKLSWNTSKYALNTDYLTIYETQHKATLLKAIKNGCNVIVTRNNSATISPVISDLIENKEIERASIVVIKRSMLSKSISDLSCDQIDKDIKESLDKLGLDHLDVYMVDLSTFIQIDDQLINHLFENIFPHLESLIVKGQLSSYGLSFNQDSITNQSKSIESIIMKRKENNCNNFSFIQYPFNIYQHQTITNSPYHNGTKNIMEYAKENELVTLNSSPSLFTTPSLQRFKSVRDHSNVDIEKILKESFDLTIHMERNNPITKDESHSNKFKENKDLIGSLTWGHTLIYERSNPKLANLWSWKQILSSKIRPLVLKSVFTLFSSNINNNWGNSYLRAINSLFETYTKSLELQIFDQQRDMDQSFENHPYFKNIKITDQKQQDSTTSTSTPTPLSLYQKSLLVASKGADIIVEDPSNEDDLKEIQSIPFDSNPINNNQIIELSKKVIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR34</accession>
    <name>A0A8J4PR34_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007361</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000387">
        <property type="protein sequence ID" value="KAF2071330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR34"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="67" mass="6730" checksum="709D679FD9F9CBE3" modified="2022-05-25" version="1">MTLIKSITSLGSVQSGAISKSTISSSTGSSSFNSSSIGANQNSSLADIDIGAWLLNILGISIKAKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRW8</accession>
    <name>A0A8J4PRW8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005921</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000249">
        <property type="protein sequence ID" value="KAF2072775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRW8"/>
    <dbReference type="OrthoDB" id="24471at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550:SF2">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140860">
        <property type="entry name" value="Pseudo ankyrin repeat-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2072775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="723" mass="84970" checksum="6C5268AAE6D99A54" modified="2022-05-25" version="1">MVDINILFKRVFGNRITRDLISYYVKHSRRYSNCTIDWFVKHKLWQMLKDKMEAKEPLLLKHKNCLFEIPDYPLFKQLFIQYHDYFPLAHPKLFHLGAVNNNMEILKFLVEKGYKGYTKKAFQAAWSSKNYEMVRYLVEYIPWMHKRKLVLTNLNKSILSRDIELVKCFVQPKFIVTHLSVEEKSFITSTAFSTGDVEMFKLIYQYYPYKPCIEWFKETPKCKNMTEIVKYVIEAFPGKIKTEFLETFANATDDHCNFELLIYMVDAKLIDHTYLLEPIFSEAAFRKQELKYYDILKDEATTNLKTLTVSRVHGSMVSVKSLEEFLDLGLKVTPSCLRMAVRNHRERKTLWWDLFYALWQDLHPSLKEQKTMKSLFDISCEEGNSKLLQFLIDQIPDTNTRPKIVFNCWVELTKSDANLPFIKILLANFACENSDEIIRAIYEAIRFGSIDIVTFLFTKLSSSTLRNVDDMSETYKLAAYHSRYDILKFLIGQKLPFKNDILGKSANVAIAALLLENGHLYSPTLTYVDAVSRDISLLTFLFEYKKGKQAINPQAIKEAIAQSKFLHFKYLMDNKVEWSFHTISPEILEAIGKSGNIEMFQYLHHKNPGQPEDYGNYCFGPAMEYGRLSLIKHLIEKFDPTMSNFVNTTNFKFLILKKYSWVLEYFKPEIMNKNSKLSKIITGDFLKKVKPEKDEDDDTSFDKYYNNFTNDVKKPLFGFKLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRZ3</accession>
    <name>A0A8J4PRZ3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_007364</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000388">
        <property type="protein sequence ID" value="KAF2071314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030587">
        <property type="term" value="P:sorocarp development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000054">
        <property type="entry name" value="ABC multidrug transporter atrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002175">
        <property type="entry name" value="ABC transporter G family member 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="578"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="605"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="632"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="750"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1157"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1190"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1230"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1271"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1298"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1424"/>
            <end position="1444"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="131"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="818"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2071314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1450" mass="165010" checksum="9D244DF2A8A2F262" modified="2022-05-25" version="1">MDEVELQDIQQENSNGSSSSSTPQTPTGLESPELEVKRGKSEFTRLASQLELESHNLRIDENRKGDIEAQPEAGADTEEDFKLRKYFENSQRMAMDNGGKPKKMGVSVRGLTVVGQGADVSIIADMMTPFNKFLNLFKPSTWKASNGSTFDILHDVNCFCKDGEMLLVLGRPGAGCSTLLRVISNQRASYVSIDGTVNYGGIPSDDWRYRGEAIYAPEEDTHHPTLTVRETLDFALKCKTPGNRLPDETKRSFREKIFNLLTSMYGIVHQAETIVGNEWIRGLSGGERKRMTISEAMVSSASINCWDCSTRGLDAASALDYAKSLRIMSDTLDKTTIASFYQASDSIYNLFDKVLILEKGRCIYFGPANKAKQYFLDLGFTCEPRKSTPDFLTGVTNPQERMVQPGFEGRVPETSAEFEEVWKKSQLCQQSAQEQDDYERQVKEEKPYIAFAEEVKDQKSKTTRKKSPYTTSFITQVVALTRRHFQIIWGDKFSICSRYFSVLVQSFIYGSVFFLQDNGLNGIFTRGGAIFSSLLFNAFLSQGELPMAFMGRRILQKHRAYALYRPAAFHIAQVATDIPIISIQVFLYSIIAYFMFGLEYKADKFFIFCFILIGTGLACTNLFRAFGNFMPSMYIAQNVLTVYLIFMLTYGGYTVPYDKMHPWFQWFFWINPFAYGFKALMANEFMDQSFDCRDAAFPAGPAYSGIFDANRICPIAGAQQGDLLIHGEDYLKAALSFKTTDRALNTVVVYLWWILFTVMNMFAMEIFDWTAGGYTHKVYKKGKAPKMNDIEEEKRNNQIVQQATSNMKDTLKMRGGVFTWQDIQYTVPIPGAPDKLLLDNVEGWIKPGQMTALMGSSGAGKTTLLDVLAKRKTIGTVTGKSFLNGKPLEIDFERITGYVEQMDVHNPNLTVRESLRFSAKLRQEPSISVQEKFDYVEHVLEMMEMKHLGDALIGDLESGVGISVEERKRLTIGVELVAKPHILFLDEPTSGLDAQSSYNIIKFIRKLADAGMPLVCTIHQPSSVLFEHFDRLLLLARGGKTVYFGDVGEKSKTLTSYFERNGVRPCTESENPAEYILEAIGAGVHGKTDVDWPAAWKSSQECQLVNQELSNLENGIGLPDEWKHSDGTPPREFATGPWYQLWEVYKRMNVIWWRDPFYSFGRFVQAALVGLILGFTYWNLQSSSTDMNQRIFYVFQALILGIMLIFIALPQFFMQREFFRRDYASKFYSWFPFALSIVVVELPYITITGTIFFVCSYWTAGLDYNADTGFYFWIIFIMFLFFCVSFGQALGAVCINMFFAMIIVPLLIVFFFLFCGVMVPPDALPYFWRSWMYHLNPARYFLEGILTDVLENVEVRCDAQDFVLFTPPAGQTCEQYTEAYFTYPKATGYIEDRGDGQCGYCIYKAGSEYTNNLDWHYSNRWRNFGILVAYWVFNTSLVILFVFLTRKATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS75</accession>
    <name>A0A8J4PS75_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_006454</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the eukaryotic ribosomal protein eS31 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the ubiquitin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000298">
        <property type="protein sequence ID" value="KAF2072231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS75"/>
    <dbReference type="OrthoDB" id="472at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000008">
        <property type="entry name" value="Ubiquitin-40S ribosomal protein S27a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002906">
        <property type="entry name" value="Ribosomal_eS31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038582">
        <property type="entry name" value="Ribosomal_eS31_euk-type_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01599">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01402">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="11">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2072231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="154" mass="17479" checksum="D3B216E55424B7BC" modified="2022-05-25" version="1">MQIFVKTLTGKTITLEVEGSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGGGKKKKKKAYSTPKVQKRKPKKVKLAVLKYYKFDENGKIKRMLMECPAETCGAGIFMAQHHNRLYCGKCHSTLIKKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT51</accession>
    <name>A0A8J4PT51_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Crossover junction endonuclease MUS81</fullName>
            <ecNumber evidence="13">3.1.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_005859</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Interacts with EME1 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, D-loops, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication fork intermediates. May be required in meiosis for the repair of meiosis-specific double strand breaks subsequent to single-end invasion (SEI).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with EME1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the XPF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.22.-" evidence="13"/>
    <dbReference type="EMBL" id="AJWJ01000245">
        <property type="protein sequence ID" value="KAF2072816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT51"/>
    <dbReference type="OrthoDB" id="5963188at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048476">
        <property type="term" value="C:Holliday junction resolvase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048257">
        <property type="term" value="F:3'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20074">
        <property type="entry name" value="XPF_nuclease_Mus81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.670">
        <property type="entry name" value="Crossover junction endonuclease EME1, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.110">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027421">
        <property type="entry name" value="DNA_pol_lamdba_lyase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042530">
        <property type="entry name" value="EME1/EME2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010996">
        <property type="entry name" value="HHH_MUS81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033309">
        <property type="entry name" value="Mus81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047416">
        <property type="entry name" value="XPF_nuclease_Mus81"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13451">
        <property type="entry name" value="CLASS II CROSSOVER JUNCTION ENDONUCLEASE MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13451:SF8">
        <property type="entry name" value="CROSSOVER JUNCTION ENDONUCLEASE MUS81-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14716">
        <property type="entry name" value="HHH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47802">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10 13" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="11 13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 13" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 13" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC4" evidence="15">
        <location>
            <begin position="618"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="122"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="210"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="377"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="547"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="130"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="165"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="247"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="379"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="552"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="566"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="585"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU369042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2072816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="932" mass="105394" checksum="32AC3D730B1D90E2" modified="2022-05-25" version="1">MMSKTITTSSSSSSSTSSAKIINKENNTTTTTTTTTTGFNPHIISFLQKEIDTIVNIQRSNGQVPDYRKTIVYKKAIKSLSLYPLPIFSSKECEILNGVGPAILKKIDAFLLNTSHPIYPSGPPTPLKNILQQKHTTSTPKKSKGITLQQQPSPLPSPIKDNSRKQSTPVKSSTTVNIDNYDEEERELLLSTPVFKKALITKKKEDDQIRLPTLNNNPTKSPFKSPKFKRKYDKIASPLRPNRHATSIKLSLNSSNNSHKPSSSSSSSTSSFSSFKLGISPFKGYSNSINNNAYRSPFKSPYKRVITPLKQTFTYEQRKATTISPSPFKSPSQYKPTLTTPTKLLKKRKLDFGAVESKLADNQQEIDDSFDIFLKDYDNNNNNNNNNNNNNNNNNNNNNNNYNNNNESNSFDQKFDYIYDDYGDNLGELYLNKGIIVNSKMDDSQDLDFSFDFNPTSKKTIEEMKTISNNNNNNSLDFDDDILFGSECKNNSSNNNNKINSTKSNIDKGEDFDDFDDFDDYLNERNQTKNQKMNSIDIDELDFSFESNSDNNVKETKAKSADSKVSKKTKAKSSKVKSDDDGDNIDTNNNNDNNNNLNINNINIIPLDLSIIKFKKIKCVIDNREVKSVQERNHIQKKLEEMNVCSEIKKLEVGDFVWVGIGDDGQEYTLNYIIERKRTDDLSSSIIDGRYKEQKFRLSKSGCENIIYLVEGVLSTSSYKNNSGGNSQFFYRKQKWGAANFGLSPDTLSSALVTTQLHDNITVKETKCLDETIEYISNLTKFFESKLVFNQKGASGSGTGNNNSNSNYLNGKEKYFYKSGKQICSLDTFNSVNIKSKGLILREFFAIQLMQIPTCSEQKVFKIVEKYPSALSLYQAFKSQKKNPQELLKDIVYGKLNKKIGIQLSEKIYKLFTESVYAIDDEISDKDDENLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTB5</accession>
    <name>A0A8J4PTB5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LSM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005226</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000202">
        <property type="protein sequence ID" value="KAF2073478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTB5"/>
    <dbReference type="OrthoDB" id="368909at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="101" mass="11818" checksum="22C7A80E0A8E0FE8" modified="2022-05-25" version="1">MEVNVNEPQTDAIKKIRSWLNCFLHIETVGNRSFRGQFICLDKQQNILLKNSCEDLQPEDESIKQEQDQKQDKEKLRDMGVLIVPLKYVESIQVLEKITTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTL6</accession>
    <name>A0A8J4PTL6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004230</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000146">
        <property type="protein sequence ID" value="KAF2074448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="17903at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00821">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="871"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1447" mass="160808" checksum="03F612A133A28DA7" modified="2022-05-25" version="1">MRELNKALTLTNNGYRGSDGGRGGDGGNGVNWGQHGGHGGRGQKGERGGNGKPIDASLSILDGGILVNYLLHNGENSIKGSDLLELGNPITELQISVRGGDGGTGGRGGNGGHGADGYPGQNATQYSSGTDGGPGGDGGDGGDGGEGGDSGNGGSVHLALHQVDMDLMMILKQRIPDINPGTPGEGGVPGNGGNGGHGGCGGSSYTWTTTTTVNTENGSQTQTHYHSNPGGFSGRGGSGGAAGAFGQAGNFGAVGDFTINLTTTEGSLSYYVAPYDLEVSQIQFIDTIGYNVIEPGSDVKLMVLHKNIAPMDVGCPTPSHQAIMAFLANNEWVYCDVSSRVEIKRWILGGESVYLPRDILFSIKDIRQNPYKLPREPFSVLSTMDHECLVERVNQTFANVSKTKSPFTIEYPAKESLIKGLSCVTLFDESPIVYSIWNKSQLGIGQKIREFEMQNTGIAKSPDRVLFTTLKINLETRGGDSFINPNDFIIHDIYGNKYNEPHNGLFADVPAIEANHLKCFATTFQFCNKATKLYTRILVESFLYLGHIKNMADAQLIQSRPFEIQLTEGYNSSDFSSQFLLVTNNQSEFNEVTQWRINANALSTTLNTWNLNLYQDLDLSHTKLDGRSLLQDFRGKTMIYINNQYVQDEVLFHATQFIKPRELFNAAKFHGLNSLIIGKQFNITQELMPLNNFERVYSVFFKDYKQFLHNLKYKIPTGLGASQSLPATSALVSPQGDDVAMYLHIDKKPRYIVFFRRVLTFAIYKTHLCSYPKEKHHIQSITVGEMIENTFALTINKKIFKFDIKKVDLKKKPSQNTQELFGILHNSIRSSVVPLSTLSSIGAGGPRTSAGLRASTGLRSSVGPIAIDNSSSTFSSWLHVKKSVKSTFSKRFFELDSDKMILTQYNDSKKGSSNMVTYDLSRYSLTRGNHYGYETSNIDHIFHIIAPEIQLTFKVDDLEIYSSWITRLSPESIEYPNNFSDQSSNVREQIINGKADARGIHLSPRSPILEERAIPTITITKKLIFAKPKEKHLIKEAKKLSEKLNSRFPNQRNIVKYDYNPYKLKGSIKNLGTLEIHRSLDKTSPHIVQVLKNTDQDIRDPDQIAHYSTFYNLMKSMDFRNKLSHLDKLFASVPEPQEEVLSHIAKLSHIGMLILAIVSDITEEQYHFRDASKWRDKLSVLKIKHNLNTLNIFKRFKFEFRSTKLFNQVMSELNCHIGIVLKKFQKTSDTLLPRRRGTDVTNATTTLWQEIMDVHLCDYQLVNDKPTFKKGISSDRAKVMDKIKKLLDKQEKAWKSFALGNSLEWGYNKKKSDNDLVIKAYRSPPFSGALVDSMVKISGVMSKSEFGTHYIPPVAIGKVVEERTCFTDNATRKAFVNNIQIKDLGCLPEKVDSTLLQVVEQPPPPLPPTDTFIPFVDEKTIFITPDPILTLDSSVHTTTFEAKDSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTS7</accession>
    <name>A0A8J4PTS7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Armadillo-like helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004966</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000186">
        <property type="protein sequence ID" value="KAF2073733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="18066at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046579">
        <property type="term" value="P:positive regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904515">
        <property type="term" value="P:positive regulation of TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037474">
        <property type="entry name" value="ScaA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37516">
        <property type="entry name" value="SCA1 COMPLEX SCAFFOLD PROTEIN SCAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37516:SF1">
        <property type="entry name" value="SCA1 COMPLEX SCAFFOLD PROTEIN SCAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="428"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="804"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="111"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="288"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="804"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="828"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="838"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="866"/>
            <end position="896"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1500" mass="168375" checksum="0CB05F0466977ED6" modified="2022-05-25" version="1">MSTDSPTSPSSSSIMNRRGTFSKAKSFRRAGLNLEVNASSSGQPHVFRFLNGEEYSGPDICHSPPITPRIVPSSVNLESEEYKQVVYLGANGTLYNKYYIAIKYIGIDDSSPAPSPSSSSSSSNSSPSTSSPSTNNKASAASTKGNANNATTPAPKDIKQFLQFNSNDPIYQKFPEHLDYDNYFDYEQSLIEWKQQVQKNLGVLQLPHAMGRTYSRPKVFYDRFLRKNSEASNDDSLSFESERKLTDSDIKESNDDDNTIYSNSPSDPHNRLKEEDAKTIDGRDRSGSDTSTGSFDGSAPLDSSPIDGSPSTGSLAGFIANGTRSRSNSSTYFDHRINRSNSLSPKQTLQRLTDSGDTHYNKMMRGGSLLNMDEDRWFLSKDPWNDQLILQEPIPEIYDNFEEYEFAMRNWSTELATKTSILPPHSTQFIQLPNKDKNSGAGENGLAHSSEDNKLKGRMGKDYSALEAELSRLQFVDLHIQSPSMKRIVLDDSTIFDAIHKSSKLSINRFTHLFDDIPLVLGKEWSMLPNNLKIDISDTFDKWYRKKLISHRQTSGSYAGGVWATHLLMPNIKSGWKDSITKKQSILPPLNIKAARRLDINSEADGKRVEFNCPIPEIPINFNKLIQPNMNIQYLLGMFDASSTGPATTINLNSSLTNIGTSGSSTIGSGIGSNSNSSNLIASLGASHNGNTVFGGFEDQRNYIKTLQAYDRRLIHSFRLDHLYSWNDAKYTPVELQGQIQELESVVTQPNFDLNILWDLICNPSIYLDKFQECFDFPDHKLYAVTLPMIPAIVPLIGFSSSSTGSPTNTTGNRTPNTPNVLHLGSPPNRSGSTGSFSLGSPASSKLPLSPSHNNSLLSSGLESPRATSQNGSSVIGNLASSANGSSPRSQVLANGSTPSACSNQFLGLITSESFVNLLKFLDRSQDPLVLSKVATLVTLSLSSELKGTMIIESIIFFKDINSLYRIAKAISFFDAVPLDIFPCPSHINEILTPVIAKGSTLEVVRLVFMYYYLGIIQERLPFYSNNVGVLGFINSSKKDTAERIGININTDKDFLSRIFRALGRKSFTVSNAFLFILIQLMKMTESPSVQAFLKIKDGLITHLRDLCESKFNHSQFAAQRIFTILQEEQVWREFLFAEYSENTKKMESQLLTDLTNCDEKGKTTLLSRLVFNLCMNTLENISTPVSKHVYKFLLNEFIFYQLFNSIIKSKKINHNTENLSKLFAVLCKTLVKLSMLKNNENIKSKRNQNDSNTEQIQITPNNLFEILTFLQQHHQLSDKYANNIRTNVLVALRHLLKPADIFEILKKEANLYNKLLIPSCRDAKNPEFNRNSWRLFFQIIRYHPNHLEYLEKNKFLNQFMDIISLNAGNIVLTNALYYLTKLFALVTYETKKNQLKQQLGSSALGLGGVNIDVKYTEKDVKNLLSFFVERHLFIKIHMIYKRFSESKTYPGFVQLANLYQTISTLPICQKLLKDTIKNAEYKEGIVKVSKWFKLDENTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU37</accession>
    <name>A0A8J4PU37_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA (Uracil-5-)-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_003887</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000128">
        <property type="protein sequence ID" value="KAF2074823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU37"/>
    <dbReference type="OrthoDB" id="10250660at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030390">
        <property type="entry name" value="MeTrfase_TrmA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045850">
        <property type="entry name" value="TRM2_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010280">
        <property type="entry name" value="U5_MeTrfase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45904">
        <property type="entry name" value="TRNA (URACIL-5-)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45904:SF2">
        <property type="entry name" value="TRNA (URACIL-5-)-METHYLTRANSFERASE HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05958">
        <property type="entry name" value="tRNA_U5-meth_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51687">
        <property type="entry name" value="SAM_MT_RNA_M5U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01230">
        <property type="entry name" value="TRMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="582"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="32"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="591"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="632"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="526"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="526"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="448"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="498"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2074823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="642" mass="72437" checksum="05CACF1DEACEA54E" modified="2022-05-25" version="1">METTTNIVPESEVKAPIPVDVDMKTEDNALSTEPTTTTTTEPTITTPTEPTTTTTTTEEVHNNKPKVLMVFNLSKYMDGKKFTKFLEQHKVTYKKCKKIVGDTYGSLNYEDEKQRADIIEKLRQVKVGNKQLKVDDKEDKMENSKKRNENPDFKAEPLKSVEDICSPWHKIEYQDQLKKKEREMKDILTKMRIEIRKNSEHNLPSWLEKFSKETACPLEPIIPSATTQYYRNKASYTIGYDLENVPCVGFALGKTGNGVTAIGNPQNTPILSLKSLEIRKQFEDYIKNHSRKPYDKPTHTGFWRQLTVRDFTTGESMATIQVNHKGLSQEEIDKEKQDMKQHFSALPDPITSFSIQLYDGLSNAAPVDHPFETIFGPDYIHEQLLGCKFRVSYNSFFQVNTKTTELLYSKVLEWANVSKDTILLDVCCGTGTIGQCVSGKVKKVIGLEISPDAIQDSIANATLNNIENAEYILGKAEDTMPGLLKKFGKDEEYVGIVDPPRSGLHGDVIKAMRSFETMKKFVYVSCKQTSLVQDAIKFCKGISKSVHGTPFRPTKAIAFDLFPHTENCELVVLFERVDSITPSDDKTAAQSTTENSNSNNNNNSSNDTTTESIVGDKRKREDDNNDSNSISEQDRSLKVAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUL3</accession>
    <name>A0A8J4PUL3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Arf-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_003516</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000111">
        <property type="protein sequence ID" value="KAF2075168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUL3"/>
    <dbReference type="OrthoDB" id="10266696at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048205">
        <property type="term" value="P:COPI coating of Golgi vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08831">
        <property type="entry name" value="ArfGap_ArfGap2_3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45686">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE ACTIVATING PROTEIN 3, ISOFORM H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45686:SF23">
        <property type="entry name" value="ARF GTPASE ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="7">
        <location>
            <begin position="20"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="149"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="236"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="406"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="150"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="193"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="208"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="238"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="283"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="314"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="348"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="372"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="412"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2075168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="499" mass="55662" checksum="2082ED94CA143567" modified="2022-05-25" version="1">MDDNSSSIDTNTLASPEEVNNFFNKLVNNSDNKVCFDCEAKSPKWASIPYGIFICLDCASTHRNLGTHLSFVRSIQYDKWKYYQLKFMECGGNTNAKKFFSQNGLSDIKKLESKYQSSSAKDYKEILLKKAKKAFLDSFGVDVDSISSSNNTTTTAAAAKPTLNTTTKPSPATTTTNNPPASNNSGLIFKHNTSGSSNTSTTTKTTTLQAKSSPIVNNSKKQQILVDVSTDPDEDDFNNFFSNSPPKSSSSNTNKKKISTKKIDSKAFDDDWDSTPQQKPEPTNTSNRSNNNNRVKDFLDFQGYDQDDFDSPPSRSSNIDNMYSSSSKFSMEQKKKQSSPNQSPPYESPRDYRDSRDSRDSRDSRDSRDNRYNNSGNNNNYNNNNNYKNSESDYARKNFSNAKSISSSQYYGEDKEKPDPEKQARLSQFSGATSISSAQYYGRDESPSLRDMNAGDFARQLVLTAKSDFTSISSAVSSSGKKLSNIANNLINDLQDRYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUS8</accession>
    <name>A0A8J4PUS8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">CYY_005624</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000229">
        <property type="protein sequence ID" value="KAF2073069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUS8"/>
    <dbReference type="OrthoDB" id="21046at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031090">
        <property type="term" value="C:organelle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20335">
        <property type="entry name" value="BRcat_RBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22584">
        <property type="entry name" value="Rcat_RBR_unk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000051">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="316"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="320"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="48"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="129"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="255"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="598"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="132"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="142"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="195"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="272"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="285"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF2073069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="691" mass="80868" checksum="B0A402392378F1D9" modified="2022-05-25" version="1">MTSSNIEMLSMTIEGMRVYVTKNHKNDTYLNNCLFSFDKIMEDLKNHNIKNGNGNSNSNSNSMTNKQQMNGKDKKDENGGYPNHFKYHEEEEEEDDIEKIAIEINGLEEEGSTDIEFEDDYKYTCGKEESIQEQVKPQTQIQPRPKQQEGQPQKQQEKTSPTKLQQKQPIQQQQQQPQPQPAQQQKQQDSVINVDSSNRSKTDNNSSSNNNNNNNNNNNNKINIDNNINDNNNNNKTKISENKVEVIVIDLDDDTDQDEKGEVSLKKRKVENNPTTSTTATPKANLRESHDSESTERELSQKDELFPEEIQLMDEKMVECFICLSEVSSFDTHAVSKCEHQLCRDCMQQYCLTKIKEYEYPIMCPFFKCTNEIGLDDLNLIVDEELVVKYTEYSFQKTIEGNADIYSFCPTSDCGYIFFWEEGDTDFLCPKCKNRYCFKCRSDYHVGSSCEAYQKWAKENGKGDELFTELVSKQNFKKCPKCKRWVEKASGCNHIVCLCKHKFCYFCGETHPCKCGNDPHVRPPQNNQMPPQFNPQNALLPLQLQQQQHLQQLQLQQQQFQLQQQQQQQYQHYYPPQQVQFFQATSPDLDRMVNNQLNDLMANQRKRQQDNLQQQQQHQQQQQYLQQAQYQQTRAQQIEGEIRNLQTQIYALNTNNPQYPQLQFRIQTLNQELYNLLQPNTPQQTGKKRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUU6</accession>
    <name>A0A8J4PUU6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_003395</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000105">
        <property type="protein sequence ID" value="KAF2075318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUU6"/>
    <dbReference type="OrthoDB" id="70519at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="80"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="148"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2075318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="21322" checksum="81452AEEFE5DB7C7" modified="2022-05-25" version="1">MSSQKKCNHTHSHNDHYEHDHSGNEGTHICNHVTSSSVSQSLDELSFERGIWGSVLSGDYDRVDTLLNKNKDLSNARDSSGFYPIHYAARSDSNFRILALLIDNGADPNLQTLGGATALHRSSFCGSVKNTDYLLNLKNIIVDIQDDDGMTPLHKAYEQGKTDIIDLLLKSNASTTIKDKRGRLPIDLLKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUY2</accession>
    <name>A0A8J4PUY2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Autophagy-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_003302</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ATG8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000102">
        <property type="protein sequence ID" value="KAF2075378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUY2"/>
    <dbReference type="OrthoDB" id="6738456at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004241">
        <property type="entry name" value="Atg8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10969">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEINS 1A/1B LIGHT CHAIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02991">
        <property type="entry name" value="ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="lipid moiety-binding region" description="Phosphatidylserine amidated glycine; alternate" evidence="5">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604241-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="135" mass="15696" checksum="0D96D02334585487" modified="2022-05-25" version="1">MTLLTKSFKQEFSLEKRKSISAKIRNRYKDRLPIIVERAPNSNVPDILKKKFLAPSNMIVQNFIMEIRKHLDTSNQSNEKASIFLFVNKTNLPPSSQLLSNIYDKYKDEDGFMYIVYSGENTFGGLEPLEEEEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PV79</accession>
    <name>A0A8J4PV79_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008437</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000520">
        <property type="protein sequence ID" value="KAF2070241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PV79"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035190208" evidence="1">
        <location>
            <begin position="23"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="77" mass="8446" checksum="CD22DA8575C39B42" modified="2022-05-25" version="1">MSIKYLLVLVLVLTTLLAVANSIDTPVEAEVTVTDSGEAQGNVGLKSFCYEIGHVACLLHELTDKQCVWYGNKCRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVL8</accession>
    <name>A0A8J4PVL8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">palmitoyl-protein hydrolase</fullName>
            <ecNumber evidence="4">3.1.2.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Palmitoyl-protein hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_005113</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Hydrolyzes fatty acids from S-acylated cysteine residues in proteins with a strong preference for palmitoylated G-alpha proteins over other acyl substrates. Mediates the deacylation of G-alpha proteins such as GPA1 in vivo, but has weak or no activity toward palmitoylated Ras proteins. Has weak lysophospholipase activity in vitro; however such activity may not exist in vivo.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>S-hexadecanoyl-L-cysteinyl-[protein] + H2O = L-cysteinyl-[protein] + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="3.1.2.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AB hydrolase superfamily. AB hydrolase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.22" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000196">
        <property type="protein sequence ID" value="KAF2073564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVL8"/>
    <dbReference type="OrthoDB" id="2418081at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000276">
        <property type="entry name" value="Acyl-protein thioesterase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050565">
        <property type="entry name" value="LYPA1-2/EST-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003140">
        <property type="entry name" value="PLipase/COase/thioEstase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655:SF21">
        <property type="entry name" value="ACYL-PROTEIN THIOESTERASE 1 HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02230">
        <property type="entry name" value="Abhydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0719">Serine esterase</keyword>
    <feature type="domain" description="Phospholipase/carboxylesterase/thioesterase" evidence="14">
        <location>
            <begin position="11"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2073564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="224" mass="24395" checksum="A97DD3371EA9C940" modified="2022-05-25" version="1">MSKEQIISVLPTEKHSATVIFLHGLMDTGSGWEGAIKMIRSQGGLEHVKFILPTAPIIPVSINFGMKGTAWFDITSLSPGGNEDIAGLDKSKKFIESIIQDEKTEYGIDSNRIILGGFSQGGALTLYTGYQLQEKLAGLIVLSGFVPSLSLPSKMREENKSTPMIMYHGTADQLLNFKFGEMSFNLLKTQGVNGTFHPVAGMGHSASEQELREVSQFIKSTLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVQ2</accession>
    <name>A0A8J4PVQ2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutamine amidotransferase type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_004128</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000141">
        <property type="protein sequence ID" value="KAF2074545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVQ2"/>
    <dbReference type="OrthoDB" id="10252281at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051857">
        <property type="entry name" value="Asn_synthetase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45937">
        <property type="entry name" value="ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45937:SF1">
        <property type="entry name" value="ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="3" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="5">
        <location>
            <begin position="2"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="650"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="259"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="652"/>
            <end position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2074545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="685" mass="77773" checksum="F934A4A51D738581" modified="2022-05-25" version="1">MCGITAIISGIDILDQDNIKYGDIKPIKQDNESNTTGAKESLQDVVCDLKTRLLNRGPDSIVLHEKKIQCTNGTDNSKVDVEINLISSVLGMRGELTVQPLIDKQGNILMWNGELFDGYEIGQHDNDTMLMSHLLSTITNNPRDVLDIMVKVKGPYAFIYWHESCRRLWFGRDVLGRRSLLVNRTQDRFILSSIGSFPNGIDDSKLPLPQWNEINTFGLYSIDFPEIIQSKQVKLQSHSWDIEAELNLGITAIGFDNADDDDNDNQVDDDNSNETSTTTTTKDDIDQFHFYPFGKRTQLTIEQYSELTFDSYVDGLYNAISKSIYTRICNLPNVSNTTPKLIRPPFHQIEPTPLVGRLGVLFSGGLDSMVLAAMSDKHLEANEPIHLINVAFGVDPSVEDYDKVTDRKAAIAGYYELKAAAPNRDWKLIKVNVTEQEMEWAKRLVYELMYPAITIMDMTICLALWFASRGEGILHDDETKTRYMTTCKVLLMGSGADEQMAGYGRHRSAYARGSWPVLQGELNKDFNRIWKRNLGRDDRVLSSSRREPRFPYLDENVIEYLNNVPLPYVCDLSLPQGTGDKRILRQVGVRLKLTESTKLIKRAIQFGSKSSKQLNKNIPARITQKCGKETFSFTTPLYVPENDPSLAQSLKTKAHKVHLSARQKRLSKQREQQQQQEKVEDENIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVS6</accession>
    <name>A0A8J4PVS6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000482">
        <property type="protein sequence ID" value="KAF2070511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVS6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="160" mass="18943" checksum="252B8B259FFAAAE4" modified="2022-05-25" version="1">MSTTTRLKKIKDEKEDFIVQIEIFKDARASIDLPTEIHKYMKSKHSYDPRKTWTFPYVDHVKQVHFAQANLECGRETIEDLGSYSEFWKVKKDFKYWEFKYFLESFKLATPVSVRVTKIIIDKEQKNFRFSTGCIDDGDSNDTSDLSNTDEDDESDFSDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVV9</accession>
    <name>A0A8J4PVV9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carbohydrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000142">
        <property type="protein sequence ID" value="KAF2074541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVV9"/>
    <dbReference type="OrthoDB" id="18792at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040405">
        <property type="entry name" value="DDB_G0275255-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34411">
        <property type="entry name" value="DUF6748 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035310535" description="Carbohydrate binding domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="293" mass="33034" checksum="3B6F9C0B68871C64" modified="2022-05-25" version="1">MKLNIVIFILGILVLFVCGQEGPTDSSPSTSTTIVTNTTLAKNSNNFFYSIAQDYIKCKPTHPICGGYSMKKVNLFEDEVYVYDIRTNIHLEMDNINEYILEGYVTQGGDGKNIFVAEGAHRILPIAIESSARIDFIYYGFVQDEKAILKLNTGNITLIKSYSDPYTTGPFNLQSDWLVNRIKHRSLFTGYFDFYDDSVFKMQQVYIEMPDAGPCPPQPLFKCQADYRVSYTRNIDRCLIAQGCMKNGPCGLWVPNCQPGYTLSIYISGPNACPHYYCDPAFLTRSSGVKLPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVX3</accession>
    <name>A0A8J4PVX3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BP74 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000135">
        <property type="protein sequence ID" value="KAF2074680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVX3"/>
    <dbReference type="OrthoDB" id="2232403at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056422">
        <property type="entry name" value="BP74_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053344">
        <property type="entry name" value="cAMP-inducible_BP74-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35883:SF1">
        <property type="entry name" value="CALMODULIN-BINDING PROTEIN CAM-BP15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35883">
        <property type="entry name" value="CYCLIC AMP-INDUCIBLE PROTEIN BP74-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23621">
        <property type="entry name" value="BP74_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035156595" description="BP74 N-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="BP74 N-terminal" evidence="2">
        <location>
            <begin position="20"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="134" mass="15328" checksum="74F982DE4B200884" modified="2022-05-25" version="1">MIKLIVLLFTLILINKSLAEKAHFNVKDSSGEHFVIELTDHDLIRHARDLIYNKAKERFHVQGKIVKEKRAYNSKWHFHLDPSSISFYDTPMDSCCSTELAGIGKRLDEACTPSFLPNCFCCLSSSKVLNEIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWG2</accession>
    <name>A0A8J4PWG2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Vacuolar protein sorting-associated protein 13 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_004528</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Mediates the transfer of lipids between membranes at organelle contact sites.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000162">
        <property type="protein sequence ID" value="KAF2074157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="428159at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026847">
        <property type="entry name" value="VPS13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056748">
        <property type="entry name" value="VPS13-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056747">
        <property type="entry name" value="VPS13-like_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026854">
        <property type="entry name" value="VPS13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009543">
        <property type="entry name" value="VPS13_VAB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166:SF111">
        <property type="entry name" value="INTERMEMBRANE LIPID TRANSFER PROTEIN VPS13E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25037">
        <property type="entry name" value="VPS13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25033">
        <property type="entry name" value="VPS13_M"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12624">
        <property type="entry name" value="VPS13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25036">
        <property type="entry name" value="VPS13_VAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Chorein N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="domain" description="VPS13-like middle region" evidence="8">
        <location>
            <begin position="1394"/>
            <end position="1920"/>
        </location>
    </feature>
    <feature type="domain" description="VPS13-like middle region" evidence="8">
        <location>
            <begin position="2009"/>
            <end position="2206"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 13 VPS13 adaptor binding" evidence="9">
        <location>
            <begin position="2291"/>
            <end position="2827"/>
        </location>
    </feature>
    <feature type="domain" description="Intermembrane lipid transfer protein VPS13-like C-terminal" evidence="10">
        <location>
            <begin position="3333"/>
            <end position="3465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1351"/>
            <end position="1372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1966"/>
            <end position="1991"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="3620"/>
            <end position="3641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="3656"/>
            <end position="3769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="3880"/>
            <end position="3900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1359"/>
            <end position="1372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1977"/>
            <end position="1990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="3620"/>
            <end position="3629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="3659"/>
            <end position="3675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="3684"/>
            <end position="3702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="3722"/>
            <end position="3736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="3742"/>
            <end position="3769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="3891"/>
            <end position="3900"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2074157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="3900" mass="445575" checksum="EBCF8D88271AC296" modified="2022-05-25" version="1">MASKILPGILKKLLGKYIEGLDTLSLPFWKGEIVLENLKIKKDIFESNDLPFELQSGVIKRVVISIPWLRFLKDPIVVNVDGIFLLFGRRDIHRFSGSDATLGDKNNNNGNTKNSNTISSEDLKTIQSTLQEESREFSGLGADGFQFRFLKKILKCLRLQVNNIHICYQDFKTSPGESFSVGITLEAFSFTSTDNQWIPTKQNYEEKGSNGTLPEQFQYSMHESASSAFKNQNDNSGAKGEFEDIHNSQVVDQVTSYKLASIFNFSIYWDSMFGQTKRVTPEDMDVLLMHSIPRQKVKVKHKYLVQPVSGFLKIILVEKEIETVTNDGVFSSAMTQFNISIGLKQIDVHLMDIQYRDMLNLLSFIQDWRRVLKYKEFRPLVPIHGNATKWWHFAFKAILSDIKKKKAGETTWKEVEENRKLRECYIKLYTERELKGDLSRLETKLIEEIEKSVTYEDIILFRTISEKEFQLKRKEEIENISESGGWWLGGWFGFQNKNVSETNDKITLNSEELKFLEVFLKLDLKKLAGKPQNTILFNFSSNIKKLSINILDWRDEQSVVALSTIEIEELNLFFEQAYNTRLKFSAGSFNIIDKCSPNTKFENIIVEKHSFNGHLGSSIDLELEKKIFDLYIILNPPQEPTDYFITMKLDPVYFINSKLFVDTMIPFFTHGNQEALDSLKYSVIKRIKILQEFLISELKHLIRNQKVADINIDVSIPSVVFPESYDKEDTLLLVCNLGNFQVKTQPHNSEWESLDFSEINDQSNEGIEDVRYARYVSILKDRVKWWRDNCTLPQVDTPIETQSYLKNYVQQHRKTINQGMDEDSRYPEHMDINIDNQKTTQDTTVDDDSDWNEISINVNNAQFYDGILFTITDINVKICNFSQISNYNYLVSPFNLNTFIELCSRPYDLKLPQIKCKSELSKIQVNISDKDLYEIAKIIGKNGILGYFMDMERKSGKELADKYETVQIKTKKSKGFEIHFSLFSDSRSILNSRQKTKERLTKTTLLRFFFIAQSINMQLHYLESSTVSFQLDKLRCRSLAKFMHMNVQVDLAFIEMRDSNSITPVLSIFPHRTFDKCPQSHHRSNSNNSPRNYSNEANSKYPLSIVATSISKNSLEYDYIDFILVIQTQFVKINIPKECAMALIAIMGTIVSDANRYYQKKKSLLTVEGSLYKNRIGVHTHPKRRKMSEKLEVNEFDPNDLKTKIQFKVPSFAFTLSTIESDLISFQFEDISINGLFQLPLVDVDLVVRDMRFIDYTNMDGVYPHILISQKSQNLLNSPLVNMHIVTHSLEKAHEWGFNQLINLNIGKIQTTLLFQTINLIRNFIHDISSAIKKYFPFLFQVMAIVDGNEKKKEKSDNDNNNDNQNNNSFDNQDQIILSAADEIKDDQDRIIEIVKKKKTFTNLEIVIDNNVLIIPKNSRSKSSLRCSVGKVILTLDQMNPRTESINVKVYGVNLSSQYEDVLDPIISDPIKLDLSFLHSQETSLKDKSIETILSITKIHLTLSQYQYNLLYNIYQQNLNQTIIPPPPSEGNTVERLNEILSIKLDIKEFVELNFEDEKTNDSRFFSMLISKPYIYCSIFNNGDTFIGIKVTGISVRDKNRLIVPPEGYQEIINIGLSAEEDPTLHRMINQPFVEAPFEILSFNMESGFINIKIDIKQLNVFCDLFWMAKLITFLSPILDPDSILNINQISKQNQPLAPPPPPPLSSSHEILNSSKRTYEKEWKLYMIIELSAKSANIMIGEISTENPLVLLMSSQLGISMQFGADGMIKILLDYDCQKGLLGIKAKDESNLNISRSILAASIKEILPPKHSTHFLWFDLNKSAKLFLESFKTNIHLCFVPEGNQFIFCELNNLSLVFSGKSYKYLLDIYDNLQKIIYDSSKSKDITDESIKLRESQTINSKVDLPIPINIKRKNSIAIHNNNDNSNNNIKPINPQIERHTNQFYKIKTKSVEMIRAHQARVSEKKGSFYDPEIEGSTSGSSSSSSSVSSDPLSRAIVKKPDLSIVPHSSNQNNSNTVFTANLTFTQISISLKNLVIIVMDDLSKHKMNTPIFNIKIAYGFCKSMIKSNKIDVEFDSVFSMDYFNNRIAYWEPFIEPWLFKAMMGLGKELSFDLVSTDLLNINFTIPLMDNLSLFLQTYSKEFGYPFNLIVANGGDEKKKKRESTRFSLIGTIKKLKHKKQKSYAPFFIQNQTGSRLRYRLETHSGQPVEGISKDDSNSEARVVIIENYGTFFELNSGESSPIKFLPDVQINLDTFSQLVLAVELLGVEKSISIPMDKIKIYEYPVFLDSTSSTKLFCNVSIQKGTKYISIYFPLVLHNKTSFPIDIATVTSGFGDAQTPNYSTVIKVGQKRAPLAINRMKNVLLKMKPSSDEFKWSTETLNINEISENKDEKFSCISKSGEKIIYIKCFFYEQDGCIIISLSSMLKIKNLLPYPISYKLKTPVENIPILRKEFESTAEQESKIEVYDLPIIDKFKFMLKIEKNQDNKGEWSEVKSISEKEDNQSIQIISINDSKNLNLNVLHLNMEFISLQGERVLLLYNQYWIFNKSGLNLSTKKSYRSKDYMASVFYLNHDETVDTSNKDVVVPAEDWYEWHQKREYPLLFSFKKNKDVDNSIRLRVGNSKWSHKISITAIGDRGTLAIQSHHVGAKNMLRTYHIGISVDLAPNLKTKVVVFTPRFIFHNLHPYPIFVRQNGDHTDSTLIRIKPLDSVPFYYFVENGHLNLKYKIDHPGFDWSPPFSIQTFSDFCFSIVDGEKSPSIPCAPYLRIKTNLEVATILVLISELSEPPYKILNNTDYQISVGQKKTDESIDIKDNSSLTYVWKHPMDEHILEVNIFGQHRASFKVDKIKTFKPIEFYHEGKSVAIKVTVEAEESTRVLILSDKTTLVLDKYEETLRQSFRLHLTGVGISLINSNPTELLYVSVDEILCEYFISSFLQKLEMKIMNTQVDNQLSKVTPFSHPVLMFSEPSIPNTPFLHMRIVRSMKMKSIDYYHHVSVKIQEINVKLEEKFLYVLLEFANSLDFSFWTGNKKTKTTLHNMDMLTPIYSFDIGDGFIDDMVDRSLPSLYGKKMYFETLSIDPLSIYLTFDLSNKSGSIRSLEQAPMVRSFRRIGFVLVSFQQAHIFLNKFHLNHAFGSNEELLAPIFRHYINEGLSEVYKILGAFNLFGNPVGLVTNFGIGFKEFFVNIGRGLMGNRASFGTGGKSLAKHSVYGLFDSGAKFTASAGKAISTMSMDQRYILERQYIIGESPNNFMQGLILGARAAKTGVYRGLTGFVRLPYEGGKSQGVKGAFKGIGKGTAGLVLKPVSGGFDFASKISEGIKNSTQLNIERKRVRFPRPLFSDSPLETYDASESYGHFLFLTYIITAGKLKRIAADQINQPLMEVVSKDERYISHLIYRNKKTLLFTNKRIVCLYDTDGFRTKFDVKYAKIIGINENEKNGGRIVIKVNKSKKLGMFSKKKNKQVEATLKCTGAEKTYIYEKILEYCKIFNPTVADCEDFEIKDPIPIPPPPPPPLVQTIQPYPLQYNDPIHYFNPNNSVNNQNNKIQPIIFQNDLGPMRNDLNPILANLLAQQPNQNMEIPIWISQGFNPPKPPETLIPQRFRDPNLYNPPINTPYTTLSHSTSMPTISQQPPQMVPPPPPPPLPSHVPNTIVEEPSFKVPKTGPTTTTSTVTPSLDIGHLKAQTPSLSTSGILKTSISNPDLSKKYRPSSVRFSETDLKEIQNQIKTPSTSNSRIVTLPAPSNPIPSPVLPPNSLPPPPPPLYAPPIVPSSPNKVSPLVIQRLQYKKSTPTEITFSPKTPGINYSDKVDLALNTILEIQKLQSQQMSQLQKNQQDLQKLLIQQQSSEFQNIMNQQQQIQSIIAQYKTIAKSPSSTLINSKPSIIEKPNLEQGTPGSANM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWT9</accession>
    <name>A0A8J4PWT9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protoheme IX farnesyltransferase, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Heme O synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_007711</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UbiA prenyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000427">
        <property type="protein sequence ID" value="KAF2070961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWT9"/>
    <dbReference type="OrthoDB" id="5211at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008495">
        <property type="term" value="F:protoheme IX farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006784">
        <property type="term" value="P:heme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13957">
        <property type="entry name" value="PT_UbiA_Cox10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006369">
        <property type="entry name" value="Protohaem_IX_farnesylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030470">
        <property type="entry name" value="UbiA_prenylTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448">
        <property type="entry name" value="PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448:SF2">
        <property type="entry name" value="PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00943">
        <property type="entry name" value="UBIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="145"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="298"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="341"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="400"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="100"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="100"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2070961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="443" mass="49830" checksum="A63EFF7974292A32" modified="2022-05-25" version="1">MNLCNRSLGLFSRSIVQQHSSFIVANSASSCKLFTTSTFNRYNSDNSSSNSNSKSNLFINNFSNSSISYGSTLYKPHKETPYIPLFIQNKHQLYKRYNSSTSTNNATATTIPVEQQDTKKEQQQQETKEEINEILKKKPWWKRGFFDLIKFPITTYVSFTAVAGYIAACPVETFSMSTLAIVGLGTFICSASANIYNQEMEIKYDKMMPRTKYRPLITGEISRQTANRASYGLLVAGMLTLATVSPYSASLGASNILLYINYTAMKRESSFNTWVGAIVGAIPPLIGSFAATQTLEPIGALLFTYMFLWQIPHFLAIAEMIKTQYNHAGYKMLTITNPTRVKPVSLLHSLAGMLMPLGFYLYYPTINISEVTLAAMSLNFLLFMLPLTFPNIFKGKSQKIIYGISLVLLPLTLLIACIFRQPYIYYYDDDEEDEKSLVNSNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXZ2</accession>
    <name>A0A8J4PXZ2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">nucleoside-diphosphate kinase</fullName>
            <ecNumber evidence="3">2.7.4.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_002531</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">Belongs to the NDK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000070">
        <property type="protein sequence ID" value="KAF2076178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXZ2"/>
    <dbReference type="OrthoDB" id="2162449at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04413">
        <property type="entry name" value="NDPk_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.141:FF:000002">
        <property type="entry name" value="Nucleoside diphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00451">
        <property type="entry name" value="NDP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001908">
        <property type="entry name" value="PRK00668.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11349">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside diphosphate kinase-like" evidence="8">
        <location>
            <begin position="73"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="6">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2076178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="221" mass="23699" checksum="3C86BBE4E8EC2887" modified="2022-05-25" version="1">MFSRLFTRAIPRAFAASGYAKRAFTTAATGFVKPSVSKKLVLGATLATTAIATTGLVACADKTPLVGVPGTKFERTFIAIKPDGVNRGLVGEIICRFERKGFKLVGIKILHPTKAQAEEHYADLSTKPFFAGLCNYFSSGAVVAMVWEGKGVVKTGRTLIGATNPAESTPGTIRGDLCIEVGRNIIHGSDSTDSAKAEITLWFKDSEVAEYQGNQTWVYEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYC4</accession>
    <name>A0A8J4PYC4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_002506</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000069">
        <property type="protein sequence ID" value="KAF2076206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYC4"/>
    <dbReference type="OrthoDB" id="1932233at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007203">
        <property type="entry name" value="ORMDL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12665">
        <property type="entry name" value="ORMDL PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04061">
        <property type="entry name" value="ORMDL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2076206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="17712" checksum="24C3FB307B791DD6" modified="2022-05-25" version="1">MDEFQINRNQSWMHSRRFWVVYIFAIIISKLTISLLFPHYEYGWLFITASHAIVTFLGMHWQKGIPFFFPANQGKYNRLTLWEQIDKGQQYTPTRKFFTLVPIVLFMITLHANGYTGIPFYINAISSFVVVLSKMPKMDRVRLFGINKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ08</accession>
    <name>A0A8J4PZ08_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000328">
        <property type="protein sequence ID" value="KAF2071906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ08"/>
    <dbReference type="OrthoDB" id="18757at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053369">
        <property type="entry name" value="SrfA-induced_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32256">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32256:SF6">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035152224" description="EGF-like domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="396" mass="44170" checksum="B5E04718D4FBC0A3" modified="2022-05-25" version="1">MKFIVLLVSLLFFINGIFGNTCTIDSECGWRRSCPNSLEIHAGVGIDHSENKIYVMGVSKSFANTVIASIPINGGPIQIEYEITTDFMDLSALGIFKYLPKSKLLYTINSQRLGLMIGLYEKNGTLTSIWNARGFKFQADFNEHKQETLTCGFGIYKYESIPKTSGEANKQLYKNVNCLGLGRLDNFVYGVKAIETGSLFFKGSLECKDREESELSHLFKEPLQVSDMAVTSTHIYYSTMAGNGTDGIYEMPLSCDHTKKRLLVKDTTKRISLHNGIIYYATEGKLKSVSISSCESKTLYCNNGKYQGECACAKNYFGSECTKCNGQEVWDPKGSPNCLITDKYSITQCIYQWNCNTPFGYCDGANCRCRSSFYGSKCDKCDGKVTYKDGVPSCEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ16</accession>
    <name>A0A8J4PZ16_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Prohibitin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_000868</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the prohibitin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000018">
        <property type="protein sequence ID" value="KAF2077823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ16"/>
    <dbReference type="OrthoDB" id="275637at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03401">
        <property type="entry name" value="SPFH_prohibitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000001">
        <property type="entry name" value="Prohibitin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000163">
        <property type="entry name" value="Prohibitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222">
        <property type="entry name" value="PROHIBITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222:SF0">
        <property type="entry name" value="PROHIBITIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00679">
        <property type="entry name" value="PROHIBITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035294471" description="Prohibitin" evidence="3">
        <location>
            <begin position="24"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Band 7" evidence="4">
        <location>
            <begin position="24"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU366048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2077823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="270" mass="30063" checksum="E360123221723F4C" modified="2022-05-25" version="1">MNFLNRLIPTALGLGTILSLTQTSIYNVDGGQRAIIFDRISGVKEKAIGEGTHFVIPWLQTPYIFDIRTTPRNIRSETGSKDLQTISISVRVLFKPDIEKLSTIFSKLGMDYDERILPSLGNEVLKSVVAQYDASELITQRESVSREIREGLTKRAKEFNVLLDDVSITHLSFSQDFTNAIEHKQVAQQDAERSKYIVLKNEQEKKAAIIRAEGEAEAAKLISTAMASGPGFIELRRIEASKDIAESLAKSKSVTYVPEGGNMLLNMNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ35</accession>
    <name>A0A8J4PZ35_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mitochondrial substrate carrier family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_003025</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000090">
        <property type="protein sequence ID" value="KAF2075652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ35"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="130"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="231"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="366"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="88"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="459"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="11"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="467"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2075652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="486" mass="53678" checksum="A4EA668C69EB919E" modified="2022-05-25" version="1">MSKAESRSLKKQNNQIPTTQSHNISSSSKSGQPYISKYYGNLVKNGKVLLSNNNISTTKLIAFSNQTTTFAKNTINKIYNSNNKQLIQSRPFTTTTPKPTRNIPNINESSSNSSNISRSILKNEKSKVKNQNLNYLLSGSIAGAISRSATAGFERLTIIQQVQGMMINDVNQRYIGCIAAIKQMLKKEGFLSWFKGNGANIVKVSPNSGIRFLTYEFCKKQFLQDPDTEKLSVPQTLASGAFAGLTSTFFTYPLDVVRVRLSLQGSSSHEMAATRYKGILHGFTKIHAEEGIRGLYKGLGTAIMSVAPWVSISFASYEGLKKLATTQTFYDHFYGKDSSLSKELNDSEKLNLKQQQLEIKSNSKGKDMIIDFICGALSGAVTMTICYPLDVLRRRMMVQGIGGSGQVIYKNGLQAFKKIFKTEGVGAFYQGIIPAYFKVVPTVAISFAVYELCKGSIGGQQHHSTENNNTNNINNNKNNNSDENDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZM4</accession>
    <name>A0A8J4PZM4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_001732</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="AJWJ01000043">
        <property type="protein sequence ID" value="KAF2076956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZM4"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF13">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="11"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="111"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="111"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2076956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="630" mass="71218" checksum="9BF0FAFE82A15089" modified="2022-05-25" version="1">MNPPTDPLAGYKIIKVLRSGGEGKAILLSKGETYYVCKERAFDSLIEATAGLNEAYALASINHPNVVKLEGVSVFEREKQVILCIIMEYCPKGDLLDFLLEISDTISEISDQTSDTDSVSSKHSSTDDLASLSISSEPTSSSTSSKTSPSPNKHNNNNNTTAQTCTGFKINKIHKKEERKCIIKMTMDLTKAALGFQKKKPNGDIEIREETIESSEFKKNGDSKFLIEQNQLIEWILQLFYGVQALHKQRLIHRDLKSENIFIASNNSLKIGDFGLAYKTTSKAINVKGAVGTYPYSAPEVLEGKAYDKSADIFSLGCILFEMITLKNLVQNRFYFGQEMIDDTFCKMGFISNFPQKYERLSPIVLRMLDKNPNLRPSIENLIEQFENMDSSTLREDILLKQHQQHGSSKNFKGMSKQLERQHFAEASKVLAQAFLVDPRFSSIFPPDQPTSLGHLTALFEYLLKVIAKKAVIWGAFTLDNRIVSVMVWFNPDKQKKIKITDVIVGGINFLTRYGLKRVKQVSHLLKFIDEILVSTNKTKEKYWFLAYVATLEGYRNTGVGTDMLKHITNWADHSGLKCKTFSFANNSHSYFNRLGFETINELNKENTKDLPKGVDNVWILERNPKPLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q062</accession>
    <name>A0A8J4Q062_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutathione S-transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_002394</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000066">
        <property type="protein sequence ID" value="KAF2076279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q062"/>
    <dbReference type="OrthoDB" id="19091at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.550">
        <property type="entry name" value="Membrane associated eicosanoid/glutathione metabolism-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023352">
        <property type="entry name" value="MAPEG-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001129">
        <property type="entry name" value="Membr-assoc_MAPEG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35814">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35814:SF1">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01124">
        <property type="entry name" value="MAPEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161084">
        <property type="entry name" value="MAPEG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2076279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="18243" checksum="AAD006F6868B8EB3" modified="2022-05-25" version="1">MINLVPITGTFMGILGGYYLYLSARVIKERVRLQISVGDGTSNVVNALIASQKSGLPIDEKELPKKYTDLVGAIRSHANFGEYVPMVGVLALILELHGANPLFLKTMLGLFTAARVLHVEFGMKAKNYLRWGRVTGTMITFTTIALSSIGCIYLSNEHTFKALLKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0B1</accession>
    <name>A0A8J4Q0B1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Swt1-like HEPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002383</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000065">
        <property type="protein sequence ID" value="KAF2076327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0B1"/>
    <dbReference type="OrthoDB" id="21574at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041650">
        <property type="entry name" value="HEPN_Swt1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18731">
        <property type="entry name" value="HEPN_Swt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Swt1-like HEPN" evidence="2">
        <location>
            <begin position="80"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="388"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="431"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="481"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="575"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="215"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="431"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="488"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="575"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="586"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="608" mass="71154" checksum="4B45092CF86960AB" modified="2022-05-25" version="1">MHLEITLKNLDEWGIGSNQLMASKALSICRDFLIFKLSELLEDNTNPTKNNSNSIDLSIQDINEIKRLVQLQGKQRKAKSNSPNGSSDDSDNHLDDMDLLDIIKVFSRKISYYVSDSTTVKYWLHEIYQFRNGWAHQRSFSLDDTNRLLDTLERLFYTFGEGNMAFAIQTLKHTHYFHTFNKIYDLLSLEKESNLYDIFKFISKINNNESDDLNNIKNNHSNYNNNYNQNSHNNNNQNNQNNNMNIQNNNQNMNNNINNNINNNMNNNINNQNMNHNIFSNFNMNNNNNQNTNNNQNNQNMNGNFNNNVNNNNQNINNNQNINGNFSNNNNNNNLNNFNNNNHNNFNFNNNTNNMNGYNDDFNFDDSYNNNYQTMLRDVHMSYENSNFQQQNQQIQQNQFQNQFQNQQPQFQNQQNQPQFSLVDHAMLHQQQKNQNQNQNQNQNQNQNQNQNQNQNNNQQSFQFHNQPLSDVDFNDNKKKRKFDEKTQSPNMNFSQRINSNNNSMNMNMNNMNNNMNNNNMNNNMNNSLFNNNNINNNSNNNNMNNNTSLKNMSNNSVNFTTPSVKKPAAVHSILGNTNNTSQGVGKKKKPKKKKGSQNEIDNFQKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0M8</accession>
    <name>A0A8J4Q0M8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000024">
        <property type="protein sequence ID" value="KAF2077601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0M8"/>
    <dbReference type="OrthoDB" id="10653936at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="410" mass="46345" checksum="4D3425EF561DBD1F" modified="2022-05-25" version="1">MIEQSASLTNTIVSLYTDEEVFQSLIANRMITNLSGMNLIPSVEVQNKMIELLKTTDQTSIVNSTIISLQNLGKNTGFYIGIKDWDLLINVVSSINHYFTDFQILESSIYPFQSALAIFQYLVKAYEDLFQKDDISPLQLILDNRKRDLLLQNLMYIINICDPNTFNLCLKLLDMLLVLYEELVLVSMAPEIFTQYKNLSNWRKAYFVRSLSNGSAKCKIIAYIISLVSAPSSLLKYHTPMDQLCNLLRGKPTYSTTHHSTPINYASVTFNSTGRRVSQLNNNNTATSSSTSTTSTATNTNTNTTMRAAANAKKETNTTQSFLSVEQASTNFPTDDEPLGQLELYLILMNQLLQCVLSIKTLTTAEHDRIVKESETFFKMAANNKPKMENQVEYDTNISMMKYLIESNKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q109</accession>
    <name>A0A8J4Q109_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_001936</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000050">
        <property type="protein sequence ID" value="KAF2076747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q109"/>
    <dbReference type="OrthoDB" id="418495at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015038">
        <property type="term" value="F:glutathione disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03419">
        <property type="entry name" value="GRX_GRXh_1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000093">
        <property type="entry name" value="Glutaredoxin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011767">
        <property type="entry name" value="GLR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011899">
        <property type="entry name" value="Glutaredoxin_euk/vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014025">
        <property type="entry name" value="Glutaredoxin_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02180">
        <property type="entry name" value="GRX_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694">
        <property type="entry name" value="GLUTAREDOXIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694:SF18">
        <property type="entry name" value="GLUTAREDOXIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00160">
        <property type="entry name" value="GLUTAREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00195">
        <property type="entry name" value="GLUTAREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="5">
        <location>
            <begin position="14"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2076747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="99" mass="11171" checksum="33362E71353800EA" modified="2022-05-25" version="1">MDKVRALIRAHKLIIFSKTTCPYCIRVKNLFKQLGVNPFVFEIDVEGDSSIQPALYEISKSRTVPQVFINEKFIGGCDDTMSLNNKNQLVPLLKEAGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q152</accession>
    <name>A0A8J4Q152_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002536</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000070">
        <property type="protein sequence ID" value="KAF2076182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q152"/>
    <dbReference type="OrthoDB" id="21485at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="380"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="548"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="381"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="562"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="573" mass="65065" checksum="466E2768A1ACC1FC" modified="2022-05-25" version="1">MELLVSEPTFSFCGVEGNIGSEDLIRSCNISVNQQSYKYNQDFSLVQNIGNDSLLSSLSNTGNDHCSNINGDDIINVCFSELENCSVPSINKDDIDQLQMNFTNPNENNGDSNDCTINCNNSSNINNNINNQNNDKNNNRIQENDNNNNNEMQYLQVINDIYADDIASPSSCHSTISINSQNMFESVQSPQSQTIPLSPMICSNLGSPVQHSFLNNNNNNNNSNIPIISKKRNYEHDNNFNFIVNPPKPTTTNNNSNVVSNYLKLQIVSEDYRFSLFSPSFIKIKDLKQRIFKEFSLLFEPNCIENNNPLHFSNIELTKLQDEFNNDLSENSMVYELLNDSDCVYATIPKFPIVYFNLFPELAPQDLIDDINRSFKKYKTSNNSNESSMVSSPLTPYQSSPIQFDSTIPNSPSINEKSTTTPKKSCNTTQNQNNTTTTTTRCYPYCEVTQPFQRNCECSKCHEIRPHILQAKENEKNLCTKWCVTSQGRKQKITSFEFCSICLSRFRLQKYTTKQSHQCTECQRPWMILVNDGKCRRCTKEKERQLKTPLPSNTATLSPSPSTVPPTPTTITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q171</accession>
    <name>A0A8J4Q171_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_002453</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000068">
        <property type="protein sequence ID" value="KAF2076212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q171"/>
    <dbReference type="OrthoDB" id="10264376at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051858">
        <property type="entry name" value="WD_repeat_GAD-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16017">
        <property type="entry name" value="GASTRULATION DEFECTIVE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16017:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="250"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="351"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="400"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="446"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="52"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="616"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="674"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="73"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="90"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="155"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="206"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="636"/>
            <end position="649"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2076212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="701" mass="77782" checksum="0D50D511CC6885EA" modified="2022-05-25" version="1">MSSVKRLRETEINEEKDENKEIKLDENIKDIDPKFLQPTAKVNTDVEFQSNTATLKKNTPATTTTGGGGFKFNISSIKLGSQQPQTNDNSKNEEIQEIKRSQDYRETSVNVGPPRPASGNIGPSKPANIGPSKPSNIGPSKPANIGPPKPSTNKDEEDSSDSDDSDNDNSKVNNLGPPKPSTSNIGPPKPSTGNIGPPKPSTTKDDDSDSSDDSDSDSDSDLSDTDEEKEEINRWVELAKSLPITSEVLIKGHHRPVSTISLDPSGSRLLTGSYDCKVKFWDFNGMDSSLKSFREVEPNEGTQLRSIQFNLAGDMFLAMPYTTQIKLYDRDGFTKGETVSGDRYIQDLNHTKGHISTLTAGCWHPQEKEEFISSSIDGTVRLWDMNTLSKNKSVIKSRNNKGTRVAVSTCTYDYRGDVITAGGADGSISMWDKRSTLIKPKTTLEAHQDQSNVTSIQYNRDGIHMISRAMDHTLKVWDLRNTATPLAVWDDLMNDYMETDCIFGSFDRVIVTGTTAPKGGFGTIVMYDLASLSKMRQIRVDDNCSVLACAWNPRINQLFFGCSDGTTRVYYDAEKSVKGIKLCVAKAPRVKDPTDFEPERPILTPHSLPLFRQANAKKEEKRARNSKQTKKPEQPVTMTSTAKGPNLTQHLIKNTDLVNDTSWQQDARESFLAKADLEPKFFKNQPKTILDTSKQEDEEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2E2</accession>
    <name>A0A8J4Q2E2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_001938</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000050">
        <property type="protein sequence ID" value="KAF2076749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2E2"/>
    <dbReference type="OrthoDB" id="1929172at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035268476" description="GOLD domain-containing protein" evidence="10">
        <location>
            <begin position="23"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="11">
        <location>
            <begin position="32"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2076749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="207" mass="23810" checksum="8E6B7B503932208C" modified="2022-05-25" version="1">MKYTGLVLFAIIASLVICTTDALTLQIEPKSQECFFNFIEREKTTLILYQVTRGGLLDIDVRIFDPRGNTIFSKLHFDTTMKGRQSFVAAESGAYKLCFNNEMSRFTAKVVSFSWTVEDSNNNDIAKGDSLTPMDQSIQKIERTLQSIIHEQKRLRYREQANRDTSESTNGRVVWWSIAEVVVLVVMGVAQIWYLRRWFDSRSSGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2M9</accession>
    <name>A0A8J4Q2M9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000554</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000010">
        <property type="protein sequence ID" value="KAF2078170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2M9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="381" mass="43642" checksum="B7E3C3D0E8CC8A52" modified="2022-05-25" version="1">MTGTLDNRFYIIDIESGNILKEFDMSKNLYFQSPHIKVGFFDAIKGHFYLALSFRNDSILLNKFSADDFKILHQVMIIRDSFYLQNNVIGINHERNGLYLVSAKHTYKFNLHDLFMQYKKPIPSCHSYSSVFHSESEIILTCYSPDKPSRLIFFNESIGGNGEFREVDLGIMIPKSIVHLEKWNVVLVPNNQMDDLSESLITLFIGDHKHIFSLGYYGLSQIGISNGKSHALFTFKNLGVLVQYKENRLHVNDVSNLGPFYITSAIYNQHLDKIILGTNSSTILSVSFKIPHDQKEADKHSTNSHKHRSHPLEWWKILFIVIGGLVSLGLIGYGIKKFKYGYSSSILDKMIIVNDKAYSDNPPTRPPSINYILKDGEKYEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2X5</accession>
    <name>A0A8J4Q2X5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001666</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000041">
        <property type="protein sequence ID" value="KAF2077034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2X5"/>
    <dbReference type="OrthoDB" id="415359at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019311">
        <property type="entry name" value="Fy-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16525">
        <property type="entry name" value="PROTEIN C12ORF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16525:SF0">
        <property type="entry name" value="PROTEIN C12ORF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10154">
        <property type="entry name" value="Fy-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="317"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="247"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="322"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="417"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="697" mass="78596" checksum="C8D5C5D78EBF65F7" modified="2022-05-25" version="1">MKIVQNDISFDLSFPVGKKFVMAEFLLKAGEEVSSAVDQFIDEYDIPIYLRTNLLSTITSLLESESSNIKDLQDKSFFENIDKSTENIKIKSKHLANQYTEKTVYWNKFLKNQETEEQLTIEKRINTFLYLVNKSNDTKERIINQEELFATEFSLLLGKKYDDIRFMNEKHSKEMEHAFKNLLETDQIEKIVASNMNEMELLEANYDQEINDMKVKQKNDFTKFLDDLYWLEKTKDDLEDIEKFNEPTTTWTSAPSTNSNNAANNPRRSSMSIGGIKNLEQQQQANNNTIESAKTSIIDSFDRGVQRASSFFSFDRFKKNDTNTQQQPQQQQTGNSNSGNEKTPVKTSPSTPTTPTPSTAAQPIPTSNNSSSGNNSTSPNGSLSISPNRNRSNSKSKFSTSPNHSSNEQMNNHRKQSIGSSGTGHHSTIQNLKENYVISVGVQKKKLFNFRLIEESPLQLCRPISTGEYKKSKRMDYIQTLYSDSLSALILLVDPELSFSSQIEKNWIKYCNMSTELHFDPVDKQIEQVKQSLMNSGSNDSGYSKTPILHNGDFFITKHSNLSDVQVVFHLVVDSKHRTPLGDSLLPTTSDISKGLRNILLTASKYGIGKITIPIALTDSELDLNITNPQLVPRTVSVLSVTRASLTSLHEMTSIKTIQFSLPPCLDSGNTNASGPIATKWKSAINPFIVNNIDFNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2Z1</accession>
    <name>A0A8J4Q2Z1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SIS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005498</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000221">
        <property type="protein sequence ID" value="KAF2073181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2Z1"/>
    <dbReference type="OrthoDB" id="311172at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070095">
        <property type="term" value="F:fructose-6-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042593">
        <property type="term" value="P:glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009750">
        <property type="term" value="P:response to fructose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1080:FF:000004">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000087">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054017">
        <property type="entry name" value="GKRP_SIS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005486">
        <property type="entry name" value="Glucokinase_regulatory_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040190">
        <property type="entry name" value="MURQ/GCKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10088">
        <property type="entry name" value="GLUCOKINASE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10088:SF4">
        <property type="entry name" value="GLUCOKINASE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20741">
        <property type="entry name" value="GKRP-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22198">
        <property type="entry name" value="GKRP_SIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22645">
        <property type="entry name" value="GKRP_SIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01272">
        <property type="entry name" value="GCKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SIS" evidence="2">
        <location>
            <begin position="60"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="616" mass="68529" checksum="0650995DBBB6B025" modified="2022-05-25" version="1">MSSITERTNELSSELDRVDSRGICKLFRQCDAQIFTGYSDYESINDSSIQNEIIEFVECVVDRLRKQTVGKVNIVATGAGTSGRLAFFIARSFNRILGDDSERITFSYLIAGGDLCLTVGIEGAEDNISQAIVDLESQINPDDTLVYIGITCGFSAPYVAAQLDYLLNLNSDKHIISLMGFNPVALARPLVIEKWTRSFKDITNQLESNRVTEQQKSKLLRRHFILNPVFGPEPLTGSTRMKSGTGTKILLESMFSLILSQYSATKYPLTIKNTSHGDSVHEQLISILKAYEETFRDTYLNIDSIAPMIDSVGNSLRAPNHSGHLYYLAAQSLGIVGFIDASETVPTYGADSQDVNGFLLDDKSKQASGWNVMANRDGDLSNVSVEYKISQKDFIDKIPSMSAKDTIVISVASLEEYNQLSTDILPLLVSNSGNNVPTIYLIQFINAKNQYSIPSHSAIKNIVIKLNHCDIIANVNSFYEISLKLIHNALTTGGFVLAGKVYGNRMIDLGLTNNKLYYRSCGIINSIMKLNNEEKARHYLLRSIYSIDSDKEIPSELDSLPVYKHIEYAEQNKIKNVVPVGLLLASGKFQSPSNIREEIQKQPIIRYLLDQNKPTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2Z4</accession>
    <name>A0A8J4Q2Z4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide-like helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_000777</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000016">
        <property type="protein sequence ID" value="KAF2077899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2Z4"/>
    <dbReference type="OrthoDB" id="433738at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF8">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="153"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="539" mass="61799" checksum="7E71CC36D6070542" modified="2022-05-25" version="1">MINRYLKVINKSKFNTIHNSNNTRLVATQIYKFPQNNKFSSITIINNNVSKTLNQSKLHYVKRDYSTSINSSTKTILNNDPLPKQYKQYIENALKEFQARDHSLAVEYLRSAIKSSPNLPNAYALMGDIYSFNRVNMGAIEYYEKAFAIKQTESVALSLGFLYSMGGEFEKAKQVYEKIIQINPNNDFAYYQLLKITGQRKFVDNLKEMNSGYYSIAKSMIATTDKEIMDKLGDFILEQDKLVASKPIVEAKKYTIGIDSSGYQRVLEADVHGTTPHLEALIVLGSMLMESNPQKALKVFSRCQDIHLFVELAYPQICFTLEEHGVSSEDLLQFLDRYIDAHPGKMVDPQLWLIRVDLLLHLNRLKEAIKVFSILIPYYILSEDQSLPFIIGRLSTADMAIRCYGEQLGQLLQQDTSDKHKQFFSPFRSYFSKENMDLFSLKDTGKEYNILLNQFTCIHEAYSQFAKDSKAQQIIDSDPLNADLHDALAGSILWCYQALSDLKIMKEKGYNKIPQLQPEMILGVVEVYFQNISQIIKGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q327</accession>
    <name>A0A8J4Q327_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphatidate cytidylyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001571</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000038">
        <property type="protein sequence ID" value="KAF2077124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q327"/>
    <dbReference type="OrthoDB" id="5673at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037997">
        <property type="entry name" value="Dgk1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31303">
        <property type="entry name" value="CTP-DEPENDENT DIACYLGLYCEROL KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31303:SF1">
        <property type="entry name" value="CTP-DEPENDENT DIACYLGLYCEROL KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="231" mass="26134" checksum="00F74F07A7E2B402" modified="2022-05-25" version="1">MLLFTKKQLTNLLRYLGIGVKRVPRVIKELRRKGFHVVGLIIPLVLYVGINYTTFFTKEVALWIMSTITLVYFIWEMLRLNIPAVKEFCMRTYGGMMREKEKDRINGSFFYLLGSTLCIYAFNPTVTIASLLFLIIGDFSAALIGLSYGKTKIGNKSLEGTCAMFLSCLLISLVLFRDCNLGEQLAFWGSLAASLVELFNPSWIDDNLTIPCFSGLAIHFMAYRLGTEIVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3A3</accession>
    <name>A0A8J4Q3A3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000004">
        <property type="protein sequence ID" value="KAF2078500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3A3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF180">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="518" mass="59437" checksum="5E45F30B55CC868B" modified="2022-05-25" version="1">MMSSFFLIWRNQYIRRLIRNLVCQDVFINVDDEYLCNNQQYLSLFTNRDKLEYKISIRFMGKASDYLHINTSNRYLINDVELNIEEKFNLNEIHDGVHKLKLVVGPTVAGVGQLSDSITNLSLIINIDQDYVDIGDDSLPTVVQQVSSNLPCKLQELHFRNIGCIQSTCILPLSLTDLNYGSNYSGFKWLVVPPNRVYKNCVLDLDSIESFQWLVENKFICNVNINLSIVPLLKSQQLPSHVTEVHLRRGIVEPDLLLPQTVEYLHFSNYGTPFSHISHLKLLYIYDEYPIKLEKGVLPPSIESLNLTYNQPLEPGVLPKQLTSLYLYTFNLPLCVNVLPSSLTDLYLGYFNQPLNAFVLPQKLKKLCLHMFQQPTFLRNSLPVSLTNLTLYKFKGSFDQCQPLDNLKKLHVYSLVPSLAGLLTNVKKLDLWVDSKIDDPSGACLVNTSIESLNLISKRFSTFYPNSFPPTIKYLSIVNVVIETSNVIPNSCRYLKSRNREIDPKYIPPSARYYKSIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3G1</accession>
    <name>A0A8J4Q3G1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TM2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_000538</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TM2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000010">
        <property type="protein sequence ID" value="KAF2078154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3G1"/>
    <dbReference type="OrthoDB" id="10262359at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007829">
        <property type="entry name" value="TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050932">
        <property type="entry name" value="TM2D1-3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21016">
        <property type="entry name" value="BETA-AMYLOID BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21016:SF25">
        <property type="entry name" value="TM2 DOMAIN-CONTAINING PROTEIN DDB_G0277895-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05154">
        <property type="entry name" value="TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="TM2" evidence="8">
        <location>
            <begin position="19"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="99"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="104"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2078154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="152" mass="17880" checksum="AADAACF9DCC5E457" modified="2022-05-25" version="1">MSHHHHHDDYHHHHHHHCKSTFLAYVLWFFFGVFGVHRFYLGRPVSGFIYLFTLGIFGLGWLFDIVYIPHMVRHYNNHHFPESTVVVAPSPIIYQISPEGGQRYPYQPQPYYASPYQPQQQAAYPQPQQAAYGNNGSQNYNTQPYNPPPYQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q423</accession>
    <name>A0A8J4Q423_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_000264</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000005">
        <property type="protein sequence ID" value="KAF2078397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q423"/>
    <dbReference type="OrthoDB" id="20651at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090694">
        <property type="term" value="C:Scc2-Scc4 cohesin loading complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061775">
        <property type="term" value="F:cohesin loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140588">
        <property type="term" value="P:chromatin looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034087">
        <property type="term" value="P:establishment of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071169">
        <property type="term" value="P:establishment of protein localization to chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033031">
        <property type="entry name" value="Scc2/Nipped-B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704:SF18">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN DELANGIN SCC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="211"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="289"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="366"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="154"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="453"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="551"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="800"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="154"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="461"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="506"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="554"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="565"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="590"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="800"/>
            <end position="824"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2078397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="834" mass="95962" checksum="48DA7F3F9A8772B0" modified="2022-05-25" version="1">MRGSTNNNHNHMNNSHTSSNNNSNNSNNNNSNNNNSNNSYYNVQRSRKAIIYRPNSDYDSSYSQSLSWQQKNNNYRNQNNNNNNKIIKEEINNIRNLADLNLNTTSSNNNSSNSIISSISDQDLQEKIRQQIQNQLNLHLLATAATSTTITTTTTTTTTTTTTTTTTTDDGDDDTTTTTTSADQEKETIEKPSYEENVCCKGKLPITLSRDTLFIGNLPLEITNLSFGSFLSEYHPISYHLKKDQNNNSEGYGFARFRTTEIAQEVMNSLSEMKFGTKQLKVSWATENCTLFVYNIETGASIGQIFELFQIYGELSKEECLFIHNYCLIKYKRRECAEAAVISLQNFEFGNKILTISWGDAIVQRSSVYLSFNPKDSNIKDGFHHFVFKVASKFGKINHYNIPKGIDGLFYEGYGFIHFRDSSNGEIAAEGLIKHLNSQTIEGVQIQCSFAQKKKDKKTNIRTPNNNNNSNNNNTTHNNKSGPIGMSLSFPVSPDSQSIHKQPYKQQQQHQQQQQQQQQQQQFHQLQQQQQQLQLQLQQQQQQQQQQQQQQQQPISKMNSTFNRQQQQQPPQQQYSNFNLHSLNNSSGNIPSRSNQTTSFNSKLNPIGTKSNSSSISNNNFPNNNNFNYFNTYNQSNNNNNNIIENNNDYQFNNYNLYNNFDNNSNIVSKPINQFGSSKSSIENNLNNTTTMTDYDYSILNMSNNGAVDNINNTPIINNDILQNIDHFQQVIQNNSQLIDQLQHYPSYQHYPISTIQLPYQQQQQQQQQSYNHQTINSFHNVGHHNHNHQHQSIPTNQYQYSSYQHQQQQQQQPPHIQQPHIQQPMPRSQFNNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q563</accession>
    <name>A0A8J4Q563_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000239</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000004">
        <property type="protein sequence ID" value="KAF2078489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q563"/>
    <dbReference type="OrthoDB" id="24077at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF180">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="530" mass="60603" checksum="474E3ACCA9768C7E" modified="2022-05-25" version="1">MDTAFYLVWRNSFLRSFIRYRVLKDIEIDVDQDYLIKNHRYLSLLSPKEKFAHKIAFRWKISTGDQFKQYLESVYKEFVDELDINIINDEHVEQGSREFTNIDCGSFHDGLFRLSFFVDKYTVGHNRLPDTIVDLSMYQSFYTQDPYTSVFVDGIVAHLPKDLKSLCLPNSYTFSMPMALPDTLVELNYASTYESLKHLVVSPATDAELCIAELINEKELEWVNQQSWIRSIKLSIPNYRINQTIAPHIRKVGITCQVEFGVDSFPVDLDSLVIYNCPIPNLRGILPPRLKVLALEEYPHPIENGVLPASLEDLSIGMHQHEILPYVLPSGLKRLDMTLYNYPLQLGVLPNGLKNLCFEEFNHPLTPGVLPSTLNTFSCTYFDSTIEPNTLPASLTRLGLYSYSGPLDSFGLLPNLRELTVIKLDHSLVSVISSPSITRLKLSFEDIDPSLSLSHTHIKYLHLQFHGIPRFTLTPNFLPLSLLKLKANKININSSDIIPNGCISLDTDMKDLNIDFIPKSVKYINKNKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q566</accession>
    <name>A0A8J4Q566_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004527</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000162">
        <property type="protein sequence ID" value="KAF2074156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q566"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="79" mass="9045" checksum="D61099BBD0EB5EF7" modified="2022-05-25" version="1">MSSERISKHTLKNMEMAGINKRFKAHSIRHAMASALADLNVDKERIRSAGQWKSNVCMDKHYFRSNRTIDALQEIGSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q5W0</accession>
    <name>A0A8J4Q5W0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="21">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">CYY_004184</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) + 4 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 4 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56008"/>
            <dbReference type="Rhea" id="RHEA-COMP:14356"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:139493"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) + 3 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 3 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56028"/>
            <dbReference type="Rhea" id="RHEA-COMP:14358"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:60628"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 21">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="21"/>
    <dbReference type="EMBL" id="AJWJ01000143">
        <property type="protein sequence ID" value="KAF2074516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q5W0"/>
    <dbReference type="OrthoDB" id="30822at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000017">
        <property type="entry name" value="alpha-1,2-Mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050749">
        <property type="entry name" value="Glycosyl_Hydrolase_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742:SF6">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE IA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="13 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15 21" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 21" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="38"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="18">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <feature type="active site" evidence="18">
        <location>
            <position position="325"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="18">
        <location>
            <position position="441"/>
        </location>
    </feature>
    <feature type="active site" evidence="18">
        <location>
            <position position="464"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="549"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="392"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAF2074516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="561" mass="63939" checksum="A4DEE60DAD21FFDE" modified="2022-05-25" version="1">MLDSFKKNVIAIVLISVFIIGTISILMFQNGGLAQGAPPFKDNERDNRGFDEHEDRHRTPTHPRNKRDIDVDDEDYNIDQPTKEPLLPKEGPFNRINGVYEKNKKVNIKRANQVKEAMKFAWDRYSTNVFGYDELRPLSNKPNEWFGLAITIMDSLDTLKIMGLNDEFEQGKQYVKNTLNFEKKDDKVNVFESVIRVLGGLLSTYQLTGDKLFLNKAEEFGKLLLHAFHDKDPLPYGYLNLKTKEGQYLSWTGGCAILSEAGTMFLEFETLSQLTNNPIYKEKVDKILSSFIKYDSRSPGLIPIMLKPDGTKSCSSEISLGAMGDSYYEYLIKTWIYSNKTDNRYRDLYVKAANSIIENLYSEVNGAGLVTNYYEGTSKTSSRGGQQEHLTCFAGGMFALGAAAQVSKDATQNEKYMEVGKKITETCYKSYEMSPTGLGPEKFRVDEKGRVAFGGSPEYKLRPETVESLFVLYRLTGDALYQEWAWNIFQSIEKHCRVENGYVGLHDVSNPNNKEDLQQSFFMAETLKYLYLTFTDASVIPLDQFVFNTEAHPMRIVQTKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q8M3</accession>
    <name>A0A8J4Q8M3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_001854</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000047">
        <property type="protein sequence ID" value="KAF2076826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q8M3"/>
    <dbReference type="OrthoDB" id="10256233at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF13">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="9">
        <location>
            <begin position="152"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="183"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="470"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="806"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="5">
        <location>
            <begin position="152"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="245"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="806"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="834"/>
            <end position="851"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2076826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="851" mass="97893" checksum="AA1F2DBB303BE78A" modified="2022-05-25" version="1">MNQYLLGHLGKKRNILYRLISNQSINKPTSNALFLSSLNNINNTISSSTLFQSSLNNSSSNSNNLIQINQYSSSTTSKRNHRFNNKKQEVFFDQENENQQQQQELILDEDYEESSNNSDNSRTINSKKDIIFDKPYSRRNALKRNKYGVVEREFKEMGLKEEILKGIKDLQWDKPTDIQNHVIPFIFKGRNVVFSSQTGTGKSGAYILPIIQKLSGFKDYLMKSNYGKDKILKHQQEEQDKPNSQDDDNDDNQDDFYVKEEEQEFLDDQDGEKEIEYYEKLKRYRDVLKNKEYNDKDPLALILVPSKELAIQVTKDIQQLCKYLVKEDGKTPLFRIQSFFGGVGEANQIEILEKGIDIMIATPGRLLQLCNKDVVSFHQVRFTVIDEFDKLFNLGFFPDTKELFDLLPLVKNKNRQNGMQTILGTATIKHKEMDKMITRFAPGHLLVNHNKELVAPTNVKQFFYFVNYHQKTSLLKYFLGRKGKTSLKGLKTIVFARTQQRVEKLAQRLHESGIKCLYIHADMSVSQRKDIIDQFQQNLTTQGEEIKILISTDVVARGIHIDNVEAIINFDVPHVAEDYLHRIGRAGRLGASGKSLTFISPEKLIFKVGERTVGLNEKHLMLKVQDSFGVKVKIDKVPGTFNTGIELKSLEDKSAANTSADMESIEKLAVAEILMKKKIIEPIKSPLNKDGIPRVKFNPNAKVRDSKMSKIIIDRKGQLYDLPALTDFKEGQYENVMNEFDKNRAKSRGISFEQKKVYTPLPSSIVTPRQKLKLNLDNLNDLDNIKGLGNNKTRYKFDDDDVDIDDHDHGGDNDNNDDSKQYGKFNFPKDSKPKSTSRYSKYNNKNFNKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q9N7</accession>
    <name>A0A8J4Q9N7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001185</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000027">
        <property type="protein sequence ID" value="KAF2077486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q9N7"/>
    <dbReference type="OrthoDB" id="3047760at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="14"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="183" mass="21863" checksum="46777499EB02012B" modified="2022-05-25" version="1">MITLDSSNEMPIWAVQLINRLDQITNRLDQITNRIDQTNNRLDQTNFKLDQTNNQLFQVRYMNSVNYNCNIRYINQQRLQDQRPIPLLKENYGIGNHPGLQTEGRFLPKNPQIGDVPGFFPEHHNDRLSNSDLTYLSRFYNNNFGIQEYDSLEQKNEKFYNWICYSTLDYDQTYYLQNHPSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4QBM8</accession>
    <name>A0A8J4QBM8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">[myosin light-chain] kinase</fullName>
            <ecNumber evidence="2">2.7.11.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_000111</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">May phosphorylate a specific serine in the N-terminus of a myosin light chain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[myosin light chain] + ATP = O-phospho-L-seryl-[myosin light chain] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22004"/>
            <dbReference type="Rhea" id="RHEA-COMP:13684"/>
            <dbReference type="Rhea" id="RHEA-COMP:13685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.18"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[myosin light chain] + ATP = O-phospho-L-threonyl-[myosin light chain] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53900"/>
            <dbReference type="Rhea" id="RHEA-COMP:13686"/>
            <dbReference type="Rhea" id="RHEA-COMP:13687"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.18" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000002">
        <property type="protein sequence ID" value="KAF2078611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4QBM8"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004687">
        <property type="term" value="F:myosin light chain kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001435">
        <property type="entry name" value="Probable myosin light chain kinase DDB_G0275057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001250">
        <property type="entry name" value="Probable myosin light chain kinase DDB_G0275057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="22"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00050882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2078611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="330" mass="37765" checksum="C7FF67EF836DA10C" modified="2022-05-25" version="1">MIRLFKKMGLSRKKDSKIEDYYDLGPEIGRGAFSVVRQATHKETGESVAIKAISKQHVSDSDMKRFTREIEIMKKLKHKNIIQLIEVFDSNEYLYLCLELIRGGELFDKIVEKGQYSEKDACNLVRQIVSAVEYMHQHGVCHRDLKPENLLCSADDEPEEFVRIADFGLSKIFEGGEELKTACGTPDYVAPEILECKPYDTSVDMWSIGVITYILLCGFAPFYADTHHELFQKILDLEYDFPEPEWSGITDLAKDFISQLLVINPAERWTASQCVKHPWLAMSHEDKELKSLDSAISSMKDYVKNREASYANILSKVRASQSTPNLFNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPS5</accession>
    <name>A0A8J4UPS5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_009040</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat PROPPIN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000623">
        <property type="protein sequence ID" value="KAF2069646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UPS5"/>
    <dbReference type="OrthoDB" id="1667587at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048720">
        <property type="entry name" value="PROPPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11227">
        <property type="entry name" value="WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21032">
        <property type="entry name" value="PROPPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2069646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="277" mass="30850" checksum="D664F4CEA671B6AF" modified="2022-05-25" version="1">MNLSNCKEKHNNLLFANFNQDYSCFACGTEKGFLIFSCDPFKERFGRVFDGGVGIVEMLFRCNILAIVGGGKKPKYTPNQVMIWDDYQNKCIAKLEFKSEVKAVKLRRDRIVVVLENKVYVYNFSDLQLVHQLETSINPKGICAVCPGTANVLACPGLKPGYVHVELYDHKQTQIIPAHESALSQIALNKDGTRLATASEKGTLIRIFDTATGEKIKEVRRGTNRAEIYSIAFNNDSTALCVSSDKNTGHIFDLSRQGATAAAIKEEEAQQKNRQST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQA5</accession>
    <name>A0A8J4UQA5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008254</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000493">
        <property type="protein sequence ID" value="KAF2070426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQA5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="73" mass="7852" checksum="AD93F74657026244" modified="2022-05-25" version="1">MSIFSALSSISNPTKSLNQKSSTLLSSNSSTSIGSNKSTTWIITSIGGPSTWYPDGKNEVFFGTIQQFLDSRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQM8</accession>
    <name>A0A8J4UQM8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007792</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000435">
        <property type="protein sequence ID" value="KAF2070891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQM8"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="2">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="194"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="345" mass="38886" checksum="36CAA5DFD6360266" modified="2022-05-25" version="1">MYGNPGSGKSTILSTLINIPGGFRSGISQLTGLTKAYKSITIDGVAYIDTPGLADIKDRDLAAIEIEHSLKTNQRYKLFFVVRPVEGRISPADLATINIVLQSIPQDINYGIIYNKLSKRTYVEYNNTNVLDTLHLSLLERPPHNYLLLRNLEELEDTDNVIPEDQEFKDGLNKFINEFPETFLPKEQVQPLPANEYENQLKEMEENHQKVLKEYQNQINQLQQKYDSSCVGYETETEEKSSPFEREGPSSESMKLFKVVKKTVVEKGESIDKYLRINCIKGNGQTEYGKWNFVGNHLVVTNRETKTNKSKWYKGILIIAKSGLVSAAKLGATSAVKSLIGSLGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UTG8</accession>
    <name>A0A8J4UTG8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">aldose reductase</fullName>
            <ecNumber evidence="3">1.1.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_003949</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.21" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000131">
        <property type="protein sequence ID" value="KAF2074761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UTG8"/>
    <dbReference type="SMR" id="A0A8J4UTG8"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000007">
        <property type="entry name" value="NAD(P)H-dependent D-xylose reductase xyl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="7">
        <location>
            <begin position="18"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="6">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2074761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="303" mass="34292" checksum="B20967BC2812264D" modified="2022-05-25" version="1">MSQPIIKLSNGKQVHAFGFGTWKADKGLVGEAVKTAIKTGYRHIDCAACYQNEKEVGQAFKESFDSNVVKREDLFITSKLYNTCHEPHNVRKHCEITLNDLGLQYLDLYLIHWPIAFEYTGETIKDPEFNENGLVKTVPVSIRETWTEMEKLVDAGLVKSIGISNFNVQSINDLLSYARIKPVVNQVELHPYLSQPHLKIYCDKVGIHLTAYSPLGSGAFVEDATVGEIAKKYNKSIPNILCRWAIQKGFSVIPKSSTPSRVIDNFNIFDFEISEADMQALDNLNKNYRTCDPIHFWGIPIFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UTU2</accession>
    <name>A0A8J4UTU2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010442</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001115">
        <property type="protein sequence ID" value="KAF2068233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UTU2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="688"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="897"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="688"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="699"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="956" mass="104680" checksum="C136BC140BC1EC99" modified="2022-05-25" version="1" fragment="single">MSATTLTPFIIFIALYPKDPSTAPSVVGNGSGSTTFIPNLYSPTTLDGFQDTTFADCPGDFDTNGPLFEILNENAKMKICNASENLKVLLVVSHSSLGSAGSYGVIFKQTAEKLEKLLGDKAENIFMVVTHASRNPHIMDQVTTDLNNILKQPTTSGIDDFKPILQQIISNKRFTCFCNPSEEAEDGSDYSPPSFSSVDLSRDALLQSIRGLAFKNVKGTTFSVSSSPNIQKMIDGYVNGTQVLLGAISIVNQKVIQSLNAYIDLINHQRVQVDISKLNDLLDKLTDICNTNDQKVSLFDYSKLLISKINACSYLPLINESEMNEILNCLSFYSLFSSNPIQIHPNSSWKTSILLTNLYSSFNNDIQSLMKPNLNTQDNKIFYHGYDIKLSDISSSVKSGVSDIQVVALNSIIIDCDITCPSANFSIYSPKWFIPSTAKFDLSSSPNTTKVEPSQGYQAQGNPGLAGYGGGNFYGVGEQFDKPFNLKIISQGGQGGQGGDGTQGKPAISGQRISINTHDGAVGYADPDIARTKIESMNRDSEITSSYLLNFFNKYWFTNNSPLSFVLRTYQEGNKFQSDFVSRFDYINFHVDVTRINYGEDGGIGGQGGIGGLAGQIVILDLSTNSNISSQFSCETSQGAQGVNGTQGRGGNQSLSLANVTICFSHNRGAIYTSDGIASATIRVNSSSDCSTGDSADPNNSLFNNNNQPQPTPHPNNAISSKENYISFIFQQNASFKQIDILSSQFVKNEINNNASGPNLDEIISRLFAFENNFSQKNNQDAIQCFYQQLQKDLISVAGSLTSEDPNIQTIKYLYQLMCTGISRLKTFNDSCVVIDMKVFLQSISQNISILQELKKMDTIKVYQSNYTNNIQAKITEATGFIKTLQNNITQSENEIIDNVNTIIDKISKLVDDAEKENQEYQDEIKQLKSNLIGREIVSGISTICAGISIFIPQVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWK5</accession>
    <name>A0A8J4UWK5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008890</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000594">
        <property type="protein sequence ID" value="KAF2069795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWK5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="589" mass="70306" checksum="EA804BF632B99A0D" modified="2022-05-25" version="1">MDLLYRILFRDKYLSRLVYEKIKEIHDGMYIRKYEDIVDIEWMLDHKHYGILKEKLKNNNSKLFFNFNAKSKRKLFYVPDIEIFGLFIKRFKDDIKPVDIAEVIFTNNYEKIKCMADNGYAYFENLYSFETPYTVDPRILECLIQHGWMRIDVDAPYHYLFRYTQESADVFRRHIKTQTISKAKSIKLLDWILNKSTQHMFTLFYPLFEKPLVFLPPTLESFQQILKPEIRDPHLFEYLCKECKLDETQIKELFIRNVGNDGNTDTLDIVENMIDGFNNQDIKNMIEKCFQRNSFIWLKSILNKYPDFSLKYINVFGSFTNPSDHSFYNDIELLELLLDRGMVLNESWSYNSIKYSSDNCNGFKYLWNRFSSDIQVNKNQILYNNIDITRQIVKICCIQSNHTILLFMMDQGLKELVENEISSQEYWILTNFSHKNNEKVIKTLMKLFTFKIKDYKAIVLNYGFTISTNLFKLLFPHIVHSISKSDIDLLYSKAFANCQLNKIKYLDSLGYQNSQSVLNLIPKLSSLPVEWAQYVYDRKLNLSYFHLLEKAVEANNYLLVKFLLTMYKYKNHTISTNQMPPHLKELFDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWS4</accession>
    <name>A0A8J4UWS4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">IPT/TIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_000881</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000018">
        <property type="protein sequence ID" value="KAF2077836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWS4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052014">
        <property type="entry name" value="Dictyostelium_Tiger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31341">
        <property type="entry name" value="IPT/TIG DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035224845" description="IPT/TIG domain-containing protein" evidence="4">
        <location>
            <begin position="22"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="775"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="781"/>
            <end position="867"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2077836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="889" mass="95858" checksum="73FDCFBA0C15CBD1" modified="2022-05-25" version="1">MKFSIYLFLCLILCCTTAIFAARTIVTTRSIPLPITPAKSTSFSTTFNSLYIAFENGFYWTTQENIVAKIPENTGSLVSSGSYSGTFNLYKITANNGASTSGYTCAASSSKGIYYASNNFFGSPKEISSTPASIPEASAFISGGAQFIISFDYSPYRLYQIYFGGVKFGAQTYNTAPSDLVCDFIALRCMVISDSNLDIYVITTNAAPVPTRYSLTAGTRANGVVNNAKTLVYYIRTDSSTSFTLEAYSYTGSLAFSKSFPNTALYIYGSFIKMAFDNIQGQLMIATQNYIIFCDWQGQSNYLIPNPVNDVFVGLDVMLQNVQSDNDNYLLITTPSSIYPIKYDSYKYCNYGYSSYGTCVCNKGGDSDCFFAITSATTADSGENLEVTLGGSFQNPSPQRSEITIYVGDKIVDTANINSISTNEIKFIVDFYSPSSLITLTMNGQNSTFQSTIYPDLTLAYLGQVNEILTFSGIGLKSFIPFKAVFPDQDNLEVNLNFINSTAVSLELSLIDSNQMDFSIIVDNSQPQTFSIDVAPYLIGNIPSQLSDKGNSFDVTCYYINSNPHAFINDKTISVTAKSGNVLNIQIPAGYYENLTLGNGVKNITLPLSYNTLSIESVEQTNIQELTITASEFGIDQNQVVISLSNEGLTISGFDPANGVIKVVLTDESLKGELLIEARQTKTINVNLKPNITSITPINPSYKGQTITINGLYLSTNVEFNNNNNEKSFLDCSELTTTSISCDIPQGTGLFTITSISKLLDANDSKSNIYKSEYEKVPVDSSSSESPSESSQAGSSQSTSQGASSESGSSSSSQGTTSSPTTSSPTTSSPTTSSPTTSSPTTSSPTTSSPTSSPTTSSSSTTSNEPSVAVLLQPKTVIVTLFMFIFLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UX39</accession>
    <name>A0A8J4UX39_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000471</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000009">
        <property type="protein sequence ID" value="KAF2078181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UX39"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="129" mass="15506" checksum="1495A84BE617A9DC" modified="2022-05-25" version="1">MDSLVKLNESLNTNIENVKKEQQRSKEKIELLAKANQSLHQQLTQQSKLNEQNLEIHSRVSREIGACKQDLFYFKNKFESKIIDYSSFKIINYWIDDCRTMGFELLYKHQRMIFQHQHFIQHAMEEAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXK8</accession>
    <name>A0A8J4UXK8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007631</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000418">
        <property type="protein sequence ID" value="KAF2071055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXK8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="186" mass="21216" checksum="2C4B076FBA166C86" modified="2022-05-25" version="1">MTTNTNNSNIQITISDASEKESISLNQLEWDFKQYRETVSYGGQPDTPMPSELSYDEQVESFKNATIENMQFYKDLPSPDDEPVASTQITPDEIIAKNINTVYCKITYLVQRPIIIKISFNRPKDIGNYELLSLYARAYQYVYGFFPTGNKYQVFGHALDEITFNGCSIIKILDDGSIACEYSFDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXW0</accession>
    <name>A0A8J4UXW0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">methylmalonate-semialdehyde dehydrogenase (CoA acylating)</fullName>
            <ecNumber evidence="2">1.2.1.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_007113</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.27" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000361">
        <property type="protein sequence ID" value="KAF2071580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXW0"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004491">
        <property type="term" value="F:methylmalonate-semialdehyde dehydrogenase (acylating, NAD) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006210">
        <property type="term" value="P:thymine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07085">
        <property type="entry name" value="ALDH_F6_MMSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000002">
        <property type="entry name" value="Methylmalonate-semialdehyde dehydrogenase (Acylating)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000003">
        <property type="entry name" value="Methylmalonate-semialdehyde dehydrogenase [acylating]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010061">
        <property type="entry name" value="MeMal-semiAld_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01722">
        <property type="entry name" value="MMSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43866">
        <property type="entry name" value="MALONATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43866:SF3">
        <property type="entry name" value="METHYLMALONATE-SEMIALDEHYDE DEHYDROGENASE [ACYLATING], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="5">
        <location>
            <begin position="37"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2071580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="526" mass="56727" checksum="BE6DD07DC4582A96" modified="2022-05-25" version="1">MLSSKLINTSRFFGIRNFHSSSSLLAHNTKLFINGQFIESKTKDWVEVKNPATQELVTRVPISTKDEMQAAVDAAVAAFPGWRDTSVSNRARIINNLRVLINQNMDKIAALITEEQGKTLPDAKGDVFRGLEVVEHASNVASLMMGETMENVSKNVDIYSYNQPLGVCAGITPFNFPAMIPLWMFPLAIACGNTYVLKPSERVPSASMFLADLAKQAGVPDGVLNIIHGSKDAVNFICDDPNIRAISFVGADHAGKHIHDRGTKNGKRVQSNMAAKNHATILPDAAKDRTLDALTGAAFGASGQRCMALSAAVFVGEANNWIPELVERAKKLKVASGACPGADLGPVISPESKNRIHKIIQTAIDEGANVILDGRNVTVDDKFAKGNFIGPTIITGVTPNMTCYKEEIFGPVLVCLKADSLDEAIKLINKNPYGNGTAIFTNSGAAARKYQHEIDVGQVGINLPIPVPLPFFSFTGSRGSFVGANHFYGKTGVQFYTQIKTITSNWRDDDVSSGVATHMPILGQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UY94</accession>
    <name>A0A8J4UY94_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 11">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="1 11">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 11">Tyrosyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_007071</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>tRNA(Tyr) + L-tyrosine + ATP = L-tyrosyl-tRNA(Tyr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="1 11"/>
    <dbReference type="EMBL" id="AJWJ01000357">
        <property type="protein sequence ID" value="KAF2071623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UY94"/>
    <dbReference type="OrthoDB" id="337870at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00805">
        <property type="entry name" value="TyrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.240.10:FF:000001">
        <property type="entry name" value="Tyrosine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02006">
        <property type="entry name" value="Tyr_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054608">
        <property type="entry name" value="SYY-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024088">
        <property type="entry name" value="Tyr-tRNA-ligase_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024107">
        <property type="entry name" value="Tyr-tRNA-ligase_bac_1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766:SF0">
        <property type="entry name" value="TYROSINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766">
        <property type="entry name" value="TYROSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22421">
        <property type="entry name" value="SYY_C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Tyrosine--tRNA ligase SYY-like C-terminal" evidence="12">
        <location>
            <begin position="413"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2071623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="476" mass="53136" checksum="AD5983D7485CA3E4" modified="2022-05-25" version="1">MFLHRTLVGGVTKSLFKTQYSNALRHSFYSKSQSRSFCSASVTTGANVIQELKSRGFIYQMTASEQEMIDLTNAKKPISLYAGFDPTADSLHIGNLLSLMVLLHFKRHGHNPIALVGGATALIGDPSGKSTDRPLLDTDFIANNIKYIRENITAVLGGDVQLVNNYDWNKDISIIHFLRDIGTFFRVGSMIKKDFIQNRIGNNNESGNDPNGISYTEFSYALLQSNDFLHLRNTQDCMIQIGGSDQWGNITDGCELIKKKTGKPGYGITIPLLTNSQGKKLGKSEGNSIWLAPHRTSPFYFYQYWIQVTDQDIERFLKLFTLHSLEEINAIVQQHNEAPHKRIGQKAIAEGVTTIVHGKRGLEEAISTTDLLFGNKLQESKTTTSDGFDIGFLLSRANAIEMPKSESMGVRVVDIFAKVSNMSKGQVKTLIGSKSLYINSEPITDIQTLITPEALIADQYMIFRYGKKAYYLVKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYP7</accession>
    <name>A0A8J4UYP7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="18">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">CYY_005353</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 18">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="18"/>
    <dbReference type="EMBL" id="AJWJ01000211">
        <property type="protein sequence ID" value="KAF2073335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYP7"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
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    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
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    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
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        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
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    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
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    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
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        <property type="entry name" value="Cation_ATPase"/>
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        <property type="entry name" value="Hydrolase_3"/>
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        <property type="entry name" value="PhoLip_ATPase_C"/>
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        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
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        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
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        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
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        <property type="entry name" value="HAD-like"/>
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        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9 17 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 16 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 18" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="213"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="448"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1048"/>
            <end position="1068"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1074"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1126"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1154"/>
            <end position="1172"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1181"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1215"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="20">
        <location>
            <begin position="196"/>
            <end position="254"/>
        </location>
    </feature>
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        <location>
            <begin position="1014"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="32"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="664"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="66"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
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            <begin position="82"/>
            <end position="124"/>
        </location>
    </feature>
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        <location>
            <begin position="669"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="15">
        <location>
            <position position="537"/>
        </location>
    </feature>
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        <location>
            <position position="537"/>
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        <ligand>
            <name>ATP</name>
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        </ligand>
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        <location>
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        <ligand>
            <name>Mg(2+)</name>
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        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00034036"/>
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        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
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        <source>
            <dbReference type="RuleBase" id="RU362033"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    <sequence length="1251" mass="141599" checksum="445D86107513C94C" modified="2022-05-25" version="1">MLNITIYNDNHSNFVMDLAREITFVVGRRNNISSPLKSTSSSTDIPLTVLNSTTTTTTTNNRFSIEEEEDQQEQQEEDNFLNNNNTTTSTTTPRLRKSNSSTGNNIMSSSSSSFSYSASHSPPSVGVVNGVLTSTDIEIEEVDRPNQYSPLMGGHQKTDSHSSIECDQRTILEKTVDFIKGNHIYLSRTIDLQLEDQPTKFPPNVVRNQKYNFYTFIFVIFYEQFKYFFNLYFLLVALSQFIPPLQTGYLFTYISPLAFVLSVTIFKEAYDDFKRYRRDKEANSAQYLRLTKNGFVSIPSSDIKVGHYILVETNQRVPCDMIFLRTTEKNGASFIRTDQLDGETDWKLRRSVNMTQKLATDESLLSLRSTLYAEKPKKDIYNFIGNFTNIDSSETESLSVENTLWSNTVIASGNVIGCAIYTGRETRCVMNTSTPSTKTGLLDSELNNLSKVLFALLCCLSLLMITLKGFRGLWYIYLFRYLLLFSAIIPISMRVNLDLGKTIYSWMMMRDPNIPGTIVRSSTIPEELGRIEYLLTDKTGTLTQNDMVFKKLHLGTVAFSKESLQDLENELRLSYSQAAKNNLNGSSSNNNSSRNRKNFNQKIRESITAIALCHNVTPVIEDESSHQQVLEIKKKKKKNSVSSFFSNIVKGGDYEKLSESDLKKKSDDYQGSSDRNISSSGEFLDHDSSNAISAKHQTYQASSPDEIALVKFTESIRLVLTGRELHTITLLNPMGEEEHYEILNIFPFTSETKRMGIIVRDKDGIITFYMKGADAIMAKLVQSNDWLEEECGNMAREGLRTLAFGKRTMSEQEYHNFSKLYADAKTTISDRAAKVQEAISTIEKDLELIALTGVEDKLQTKVKPTLEMLRNADVKVWMLTGDKIETATCIAISTKLVSRTQSIFQISVNDKNRALEKLNQFSRMRDACLVIDGHSLQMCLDNFKEEFINIATKAPSVVCCRCTPTQKADIVRLIKEKTKKRTCAIGDGGNDVSMIQAADVGVGIVGKEGKQASLAADFSITQFSFLANLILWHGRNSYKRSARMSQFVIHRGLIISFIQCVFSAIYYFAAISIYNGMLLVGYATLYTNAPVFSLVLDEDVPQEIAFRYPELYHELQKGRSLSYKTFFIWVLKSVYQGGAIMLLSIILFESSLNNIVSITFTSLILCELLNVATEIHTWHKYMIASIIVTLLSYLITMMIPQILAFELSFILSFEFVWKVAVITVVSCLPLYLIKFISRKIDPPSYTKLTYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZH0</accession>
    <name>A0A8J4UZH0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005664</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000232">
        <property type="protein sequence ID" value="KAF2073018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZH0"/>
    <dbReference type="OrthoDB" id="23795at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.10">
        <property type="entry name" value="Pesticidal crystal protein, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036716">
        <property type="entry name" value="Pest_crys_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35598">
        <property type="entry name" value="ENDOTOXIN_N DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35598:SF2">
        <property type="entry name" value="PESTICIDAL CRYSTAL PROTEIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56849">
        <property type="entry name" value="delta-Endotoxin (insectocide), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="277" mass="32069" checksum="69EFAD7B57796907" modified="2022-05-25" version="1">MSEPDLTVIEKFCPLAAEKLAFPAKEEYLRKADFEKKMKKLITDMENKMDKKIDDEIVKFCEAKFASLCSVGAEFAETQSIYDEEKKESKPTSESVKRYMAAYHAAFKSLLLECLIFFSREDKIDLLCKLYLQTVVMYIAFLRDTHFHGIDWGLPEKTVNGVDHIKSFAYKTNKTIELAYSYYNLQVMKGNVRKEESRISVNSVIDTRGITTIASNVLFFKCFYFTEPKNLTFRPTLIQMLKLKMVIREIPEGKHAENPDTSRNVATKSHSENFAYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V026</accession>
    <name>A0A8J4V026_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_004839</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000179">
        <property type="protein sequence ID" value="KAF2073843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V026"/>
    <dbReference type="OrthoDB" id="21075at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="8">
        <location>
            <begin position="549"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="42"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="135"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="481"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="583"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="787"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="960"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1004"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1062"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="42"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="482"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="589"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="617"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="628"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="638"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="696"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="793"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="824"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="838"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="851"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="960"/>
            <end position="989"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2073843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1236" mass="134923" checksum="19A804FD6F0D409F" modified="2022-05-25" version="1">MSNSTGTKQEWSGTRYPVICKYFKSGTCKLGDSCRFSHSYGTTGGSTNSSTAATLSNKSTTGNSSSSSSNNNSSGGNNTNASLRSTSTAASAADDKINSKKVYNSQDGTILHSPTLPPSSSTPISYVNATTINSSASSSNTITSPSLSSSTSTTASISTSTTNTNNNNSNSNLNSSMSNLSLDDNEEFISYHTRSNPNRMAGRRPFLQLVVLYAADPRVLRFSKEVQSMFLDRGIDVFLKTEENNQSIKTENLAEIITGSKADCLIVLGDRNLKNRSCQAKRRGKLVEMDIEEAITCIWNEWSLLLPKEDEELTDEQVFNLLEKHCKLRHVKDRLSKFKTSINEFKEIKLPSKNHHYQSTRNNIVENPTFLDEKIASKLTSAQKSVMRFHLQVVEAIEYVEDMPFITQTEKLTSTRGSVVGPEVSISDSKPVISESNREKLLYHLSLILVKIEKLGNVLSEFGSPLWDAYYEEYREKQQEMNNNSSAALSNNNSSNHSFSNSNSGGNNSVANSSNGNHSVSNNSSNSSNSNNTNGTSYHIDDEPVVDMKPGQWRCLLCTYINDSDAYSCLMCLTPQPTANGDNDHHGWNNSNGKSGKNSNNSNSNQKKLSVQTTYVKVDDKSKKDTKLSIQTDQPKLSVTTTPTVQTTSTSSSTTSAPTTNVSQSSSSSTTTTATTSISTPIESEPFSTPVPPPNSLSNSSTTNLNNNNLNSSLPSIPTTNNSNNINNNTIVNPLSSSQPISFSTILSKDAYSSGLYGTNALGEPSSSSFLTTVPPEHYEEDFPALSEKVGSKSKNAKVTKNGIQSPSSPTSSANNLQPPLSPSIHSINNNNNSHINGIQSPTKLKPQQQNSFNLPINSQNNNNNNNSFPQSPNGKLFGVFESSSDIINSPRGTSASFNSVVNPTTNVNGTPTKGPNYYNIFANNTWESSIFGKDSSSANGSSYNDPLFSSWSAFSNNNNNSNIETNNNNNSNNNVNSKQSNGNTSSSSLFDLNNNIFQQSPFSSGGSSSFFNQPNSASTSSSASSISSTAFPSMYSQYSNSNSLSSLQQQYQQQFFNQQPFLNSTSTPTTSSPIPTPTNSSSTSSTTTTTTSSSSSSTTTTTNSNGSLNNTISNNNTNISYNNNNIQQQIQLQLFIQQQQNQQTQQHQQEQFEREKQRAREIVNEKYKHSQPPQRPCCYCGEESMLECYNCAKTGYATYFCSKEHQALMWKEHMRVHQFYANNFQQPQQQSFLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V094</accession>
    <name>A0A8J4V094_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">UTP--glucose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="2 5">2.7.7.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_003374</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>alpha-D-glucose 1-phosphate + UTP + H(+) = UDP-alpha-D-glucose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.7.7.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the UDPGP type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.9" evidence="2 5"/>
    <dbReference type="EMBL" id="AJWJ01000104">
        <property type="protein sequence ID" value="KAF2075345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V094"/>
    <dbReference type="OrthoDB" id="932129at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003983">
        <property type="term" value="F:UTP:glucose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031154">
        <property type="term" value="P:culmination involved in sorocarp development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-alpha-D-glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00897">
        <property type="entry name" value="UGPase_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.10.10:FF:000001">
        <property type="entry name" value="UTP--glucose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000002">
        <property type="entry name" value="UTP--glucose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002618">
        <property type="entry name" value="UDPGP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016267">
        <property type="entry name" value="UDPGP_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43511">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01704">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000806">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000806-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000806-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2075345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="502" mass="56401" checksum="FE889CEC245C4E5F" modified="2022-05-25" version="1">MMKTDNQSPLPQSPQLQSFGSRSSDLATEDQFLKRLEVISQTAPNEAAKKEFLNKEIPAINKLFTRFLKNRKKVIDWSKIKPPPSEMVLNYKDLQSCSHEKTAQLANKLAVLKLNGGLGTTMGCTGPKSVIEVRSEKTFLDLSVQQIKEMNEHYNIKVPLVLMNSFNTHQETGKIIQKYKYSDVKIHSFNQSRFPRILKDNLMPVPDKLFGNDAEWYPPGHGDVFFALQNSGLLETLINEGKEYLFISNVDNLGAVVDFNILNMMDTEKCEYVMEVTNKTRADVKGGTLIEYEGKAKLLEIAQVPSAHVEEFKSIKKFKIFNTNNIWVNLKAIDRVLKQNLLDDMDIIINPKVADGKSILQLEIAAGAAIEFFNNSKGVNVPRSRFLPVKSTSDLFIVQSNLYSLEKGILVMNKNRPFTTVPLVKLGDNFKKVSDYQARFKGIPDILELDQLTVSGDVTFGANVVLKGTVIIVANHGSRIDIPEGSNFENKVISGNLRILDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1C9</accession>
    <name>A0A8J4V1C9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acyl-aliphatic-L-amino acid amidohydrolase</fullName>
            <ecNumber evidence="3">3.5.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">N-acyl-L-amino-acid amidohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_001726</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M20A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.14" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000043">
        <property type="protein sequence ID" value="KAF2076950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1C9"/>
    <dbReference type="OrthoDB" id="3064516at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004046">
        <property type="term" value="F:aminoacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05646">
        <property type="entry name" value="M20_AcylaseI_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000177">
        <property type="entry name" value="Aminoacylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.900:FF:000001">
        <property type="entry name" value="Aminoacylase-1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.360:FF:000005">
        <property type="entry name" value="Putative Aminoacylase-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052083">
        <property type="entry name" value="Aminoacylase-1_M20A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010159">
        <property type="entry name" value="N-acyl_aa_amidohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01880">
        <property type="entry name" value="Ac-peptdase-euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45892">
        <property type="entry name" value="AMINOACYLASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45892:SF1">
        <property type="entry name" value="AMINOACYLASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036696">
        <property type="entry name" value="ACY-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00759">
        <property type="entry name" value="ARGE_DAPE_CPG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="11">
        <location>
            <begin position="184"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="375"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR036696-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR036696-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2076950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="405" mass="45960" checksum="6D5E14B1DCD5F29F" modified="2022-05-25" version="1">MQQEDSSVTVFRKFLQIRTDHPNPDYEASAQFLMQKANEYGIDAEVYRETGTPIVIMKITGTDPSLPSLLLNSHVDVVPAVLESWNYEPFSGYKDEKGNIYSRGTQDMKCVCMQFLEVAHRIVQSKLKLKRNLYLTFVPDEEIGAGSKGMEVFVTHDKFRQLNVGLCIDEGLASPTEEFTVFYGERAPWWVHITAVGNAGHGSRFIENTAVEKLMRTINKMLEFRQQQFDSLHKGHHECGKKLGDVTTLNLTVLKAGINNGDNYSYNVVPTQAEAGFDIRIPPTVNLDEFLKQIKEWTAEEGLSFTFANYTEKNEMTHLNNDNKWWSTFKSSCEKIGIKLVPEIFPAATDSRFIRNLGIPAFGFSPINNTPILLHDHNEFLNENTFIRGIDIYMGIIPSLANMEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1R5</accession>
    <name>A0A8J4V1R5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Actin binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_007966</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the vinculin/alpha-catenin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000455">
        <property type="protein sequence ID" value="KAF2070712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="29742at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.230">
        <property type="entry name" value="Alpha-catenin/vinculin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.810">
        <property type="entry name" value="Vinculin, Vh2 four-helix bundle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036723">
        <property type="entry name" value="Alpha-catenin/vinculin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017997">
        <property type="entry name" value="Vinculin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006077">
        <property type="entry name" value="Vinculin/catenin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46180">
        <property type="entry name" value="VINCULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01044">
        <property type="entry name" value="Vinculin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47220">
        <property type="entry name" value="alpha-catenin/vinculin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1592"/>
            <end position="1621"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="724"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1264"/>
            <end position="1314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2070712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1771" mass="194480" checksum="BEF36167170A26D4" modified="2022-05-25" version="1">MEQLLEAISDAVSSMVLYSVEADESNAAIPNILPGAQGVKSTVDYLVDLAGKSAVLWDNFNQPDMKEKMLATCESIRESTGFLLESATILSNMPFNKPAKKTLLKGAKGIMEHMVVLLQQADLYEVTRLIRYCRKTESKLKIFIGLEPGETYFATAAQDFVTSTVDVGKIVTKRINEIDDYGLKKRFEEATSALKVEVPVVLQYFAYYQRDPNDKNNFNLGHKTAKTIFDLIEEIITVARLSAKSPFDLSMIQGLDLRDEDDLRDANILIAQEREKLLSAIQAGDGKEASRALKAIKKGLNDQIIITKALAKSADNPIQRKRLEDASLQAQRVLDSIVAQFGQAVEELLSQPDNSILLERLRMNLDTIKSASDQMVTSSARLSSNDVAEAQKILEDLVEKCKANIDRSEIDKLKVNVPLLSSTFNDVIELAESISKSTEDPLVKSLIENATVEARKRGSALIGTIERLTRDLERDPNNKDVASQLHQALIALEQVGKDVVKATSLGTSNDIYQAHNLVHEELGKLKQAILEGNKKEMQASLRAVRKAMNDEVNLAKSLLRVTEDQQMREALQAVILKADQDLNDMITDLYSSATAAIEDPNNPSAIEKLDQAINEIDQLNSNFLGAVSRDLMANNTKELEAKLTQLVHSIKNGDQQESVAVLKGIVENIKKQALFAELASGFINEQDPHRATRIKERALDLSNTGPDLVKAVKANLVEMNAANLQALAQSINTVRETNRDLNNAVLLTTEEELMENSAKIDQEARRIQANLERGLPINMNEVNSLMKKMNNQIRLAHQHANAMKDPNSKKLLLDSTNNLNKIVSLLVDACKNSAANPNDEAAKKQVKDLLDAALRANMELIGGSFSTADELVYGTPNLLKLISRLEEALLTGDPDEIKRAFKELNDELTRQLFLARIAETSITDPERRKQLLDAINDLESLQNALYPSVMDFLNNPNSLEARDSLNQLLRQLKGGVERVSTISSANPSEHLETKSYTIANELNKVQKALDKNNANEANDSIQKALAGIKQQIQLSKHIADNTNSIPQKRAIIEISDKLEKQALVLQQAVKESIANPSDAKAKAKVAQATAETRVLMAQLIAASSNKVPEEQIIATANAIKKDIDNLKQVLASGKPQEINDAIKAFKSGEALQKLELLKAYNAKVQDPYKKRQIANAISDLEKKLNDSINYVDNTIGNNNPNAEQIKQLKNLTEQASASAEHVIKASTPSHEDRILSQAVKISETLDNINSSSKKGKKADVDANVRQLREEVGDTIQLIKQASEASKDKQKQVALNEVAEKLKNVTQQIGNAANNAVDKPTDAALQKQLVDLGNQAKELLAKAVNNSSNSENPNQLLESIVFKASNDINRMASAAESGDSTFIGDAVTNLQDTEKRIKILSQIPNANIPQAALKSLEQTIPTIIANGNVLLTQPKDAAAKQSVPTHSQKAIEDLSSIISATNSAPEDKIIGNGAVINRELDQLSTNSKKPNSKPAVQSNVDAAYKTVAEQSVLARALANQTSNAARKQELLNKAKELDKLLPQLDQVNQSIQQSSSPTVDKATTDKLDQIVTNIKASNQKVVAEAINEKLEKEAKERREREERERAEQERLQREKEEREKAEQDEVAKAALKIAERTKNLAKDNTSEGKLYSTAQGIAGIMRDLSIAAANNDKKGMIQCSKLLAEHITLYLQQAKETAAKCTDPKLKEQIITAAQAAKNWTVQLKIIAAVKAASEDDDSTSNKQQLVKCARGLAKAVVHTINAVEIGQIRAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1R8</accession>
    <name>A0A8J4V1R8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001328</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the csb family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000030">
        <property type="protein sequence ID" value="KAF2077400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1R8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008601">
        <property type="entry name" value="Dicty_CAD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05720">
        <property type="entry name" value="Dicty_CAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="112" mass="12648" checksum="00F33401CBF14475" modified="2022-05-25" version="1">MSHIGVPISVSIDSNGKHSILGNGCVLPMPKIFPTPCFLMISGPAKFEGDRQIWEGRKEVKNVIVEYRGKSFPFGDCRVDVINEDFDEDGINDYRINLWPKNPVKDMPFFGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1Z1</accession>
    <name>A0A8J4V1Z1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002776</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000080">
        <property type="protein sequence ID" value="KAF2075928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1Z1"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF81">
        <property type="entry name" value="PROTEIN ABHD13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="84"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="288" mass="32956" checksum="B798C3B9F17B1A29" modified="2022-05-25" version="1">MVLNALLNTIYWGGIIAATGLACILSLIYVGQERLLYFPDTTHLLSPYIYKFDDNSYEEIFLTTSDGIKIQVWFFKQPNSKTVPTLLFCHSNAGNLSHRLENIRQLYDKVQCNILILSYRGYGKSQGTPNEQGLKLDVDACMEWLLNEPSIDPTKIIAFGRSLGGAVAVDGTARHSKNIKALILENTFASIPEMVDVVLPQLKLFKWFCRNNWNSAITIRELTCPILFLSALNDELVPASHMKTLEKQAYQCRKKTIIFEEGQHMTLMRQENYYDYIKEFMISVFGDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V260</accession>
    <name>A0A8J4V260_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000755</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000015">
        <property type="protein sequence ID" value="KAF2077955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V260"/>
    <dbReference type="OrthoDB" id="1062969at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="437" mass="48828" checksum="48C91CE2C370DEF7" modified="2022-05-25" version="1">MSPPILFNHPDHLPKFFGKLQIIGTADQEIKKYRALHATDAALLKMGQVTPTLTKVTQQPLLNCSKEVQAAYQNNDFATIKKLEAKAQSDVQPVFDNIMSLGDKAWTKVKSTETGEEYYMSWSKSAAVWSYTPAIRPSTADEIKDDPNAKFQAVAQFGTYSQTSEITGTQKFDLGLPEKAVESVISLIISQTLSTFLPQGLNFLASQFAEKLSQFAVEKGLEFFKFVIPEAWMGPVTMSVVFVLVFIGLKVLYDWLNRKYTIRVQVFNWDNQNDWQLTTQALSNAVNPGKEKNTLALNINIDKMIDPQNVTYPPDFKPNETLECTVFYAVIIYENDATFAQGCEVYIYAFLSTCAKQKRGITYAFSCPRFSDNGQYMEGAVLDPANFLKTASTKWSHDLKIKVTAAGINEPVTACMSALSGADENIYDVNIQLNKDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V322</accession>
    <name>A0A8J4V322_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_002241</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the proteasome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000060">
        <property type="protein sequence ID" value="KAF2076438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V322"/>
    <dbReference type="OrthoDB" id="429533at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03763">
        <property type="entry name" value="proteasome_beta_type_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000005">
        <property type="entry name" value="Proteasome subunit beta type-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF4">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6 9" id="KW-0647">Proteasome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0888">Threonine protease</keyword>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2076438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="265" mass="28413" checksum="0A7D6D109C8F1046" modified="2022-05-25" version="1">MEELNRGGFTFDLCSRNTLLEKTGLKMPGFLKTGTTIVGVVYKDGVVLGADTRATEGPIVADKNCEKIHYIADNIYCCGAGTAADTEMTTALISSKLKLHKLSTGKEPRVVTALTMLKQMLFKYQGHVSAALILGGIDINGPSLHTIYPHGSTDQLPYVTMGSGSLAAMAVFEAKYKTDMTRDEAIQLVAEAISSGVFNDLGSGSNVDVTVIAKEGVTVLRNYLTPNERKFRGPEYIFPIGTTPVLSKLVAPLSSKVIIEDVVMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3D6</accession>
    <name>A0A8J4V3D6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sec1-like family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006214</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000276">
        <property type="protein sequence ID" value="KAF2072482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3D6"/>
    <dbReference type="OrthoDB" id="10262287at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1910:FF:000005">
        <property type="entry name" value="vacuolar protein sorting-associated protein 33A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043155">
        <property type="entry name" value="VPS33_dom3b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="561" mass="64039" checksum="7B6360AA9B351DA0" modified="2022-05-25" version="1">MGFLNLIADPQYLQSQGAEQIFPLKRGPLETNCKNVVYIVTPDIKYMHIITDQVKQHAMDGLKKEYSLVFVPRGTILCSRVLEEEGVLGNFTIHEFPLDIVPFDEDVLSLELGNSYKEYLLDGDRTSLFYVAKSLMKFQAMFGTIPVVKGKGQCSRLVMDMITRMRKEMGEEFTVPPEIDSLILIDRDIDTVTPMCTQLTYEGLIDEIYSIKNNAIFLDETIVNPSQQQQQQAQSTGKAIPPSGKKVPFPLHSNDKVYSEIRDTNFSILCGLLNKKAKDIDEYYKKFKSTQSITAIRDHMKKLASYQMEHNSLRIHTCITEEILDITKSSSFMKGIEAEQNLLAGVNLEAVERYLEESINKKDSIYKVIRLLILYNSTNNGIKPKYLEFIKREIIQTYGTEQILTFYNLEKLGLIRKSDPKPTFNVIRKELNLIIEDLNESNPSDIAYTYSGYAPLSVRLIQHAIRPSGWRSIEEVMRLLPGPTFEEIQPLPQGATTNVKSDRKPITLVYFIGGVTFSEISALRFLSRQDQANRDFIILTTKLINGNQMIDNFIEKLTPDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3P9</accession>
    <name>A0A8J4V3P9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F5/8 type C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008663</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000556">
        <property type="protein sequence ID" value="KAF2070019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3P9"/>
    <dbReference type="OrthoDB" id="5985199at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045335">
        <property type="term" value="C:phagocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098636">
        <property type="term" value="C:protein complex involved in cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046871">
        <property type="term" value="F:N-acetylgalactosamine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070492">
        <property type="term" value="F:oligosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000016">
        <property type="entry name" value="Contactin-associated protein-like 4 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2080:FF:000001">
        <property type="entry name" value="Discoidin-1 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052487">
        <property type="entry name" value="Galactose-binding_lectin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037221">
        <property type="entry name" value="H-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019019">
        <property type="entry name" value="H-type_lectin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46938:SF1">
        <property type="entry name" value="DISCOIDIN-1 SUBUNIT A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46938">
        <property type="entry name" value="DISCOIDIN-1 SUBUNIT A-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09458">
        <property type="entry name" value="H_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141086">
        <property type="entry name" value="Agglutinin HPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01285">
        <property type="entry name" value="FA58C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F5/8 type C" evidence="3">
        <location>
            <begin position="9"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="253" mass="27872" checksum="FC859BC770181A03" modified="2022-05-25" version="1">MSVAGLVPCLSNSMLNLRTSTNYNGVHTQFNCVLNYKNNGQNGIAGSEAWCSSNIDTNQFLVAGGEVPKTFMAVAIQGRGDADQWVTSYKIRYSLDNVSWSEYRNGAAINACTDRNTVQTHFFDAPIRARSIAIHPLSWQGHISMRCELYIKPLTTSVVQSGSNVYTGDNCALNTGSGKRTVSIPVQFPEEFAKVPKVALTFDQIDATDATNQTRIGVQAENVTNKGFNCVFYTWNQNKVYSLRADWVAVSNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3U4</accession>
    <name>A0A8J4V3U4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">RING-type E3 ubiquitin transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_005774</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">May catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides thereby assisting the folding of proteins. May also function as a chaperone, playing a role in intracellular transport of proteins. May also have a protein ubiquitin ligase activity acting as an E3 ubiquitin protein ligase or as a ubiquitin-ubiquitin ligase promoting elongation of ubiquitin chains on proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cyclophilin-type PPIase family. PPIL2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000239">
        <property type="protein sequence ID" value="KAF2072917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3U4"/>
    <dbReference type="OrthoDB" id="30774at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000014">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase cyp65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF1">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN-PROTEIN LIGASE PPIL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0697">Rotamase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="14">
        <location>
            <begin position="35"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="13">
        <location>
            <begin position="313"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="278"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="476"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="248"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="214"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="278"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="476"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="496"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="528"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2072917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="565" mass="63214" checksum="34C6996C7E7F30BC" modified="2022-05-25" version="1">MGRSKSHNKQYITHTEWVNDFGGKREGDSKNNSIKPLPFNCCTLSLQPFDHDDAMSNDQGYLFDMLNLIPFIKKHGVDPISGKACTIKDYFRVHFSKNSEDKFQCPVLEKEFTDFSHIVVIKTSGNVYSFDAVKKLNIDAKHWVDLLNDTPFKKEDIITIQDPTNRKNLSASHFVQQNIDVQSIIQGSTSSLNNVNEATNRIIKELNDKGIPTTSGNTNTAVVSSNKPTTTTTATTTTTIKKTLTPEEEKVEREFEKFKKEREEKERALKKHSIQAPSFTSTGFSHNQMPTEKHPSETPGKVTKKKGYVQMKTNVGDLNIQLHCDLAPKACENFLTHCTDGYYDGVLFHRVIKNFMAQGGDPTGTGTGGKSIWGRSFKDEFVPSLMHSERGVLSMANSGPNTNNSQFFITFRSTPHLNNVHTIFGKVVGGLDTLKKIEMVSVDDNDRPTKPITIVSTSVLENPFPNVIREEFEEKLKQEKKKNQNKYDESDERGSWFSNPANISGNSNSSTGVGKYLPSASSTTTTSNKRTNQSLNLPSASSTTPTLQQEQQQKKSKTYGNFSNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4Y5</accession>
    <name>A0A8J4V4Y5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Non-specific serine/threonine protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_007371</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3/PI4-kinase family. TRA1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000389">
        <property type="protein sequence ID" value="KAF2071309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="18898at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003151">
        <property type="entry name" value="PIK-rel_kinase_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046807">
        <property type="entry name" value="Tra1_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046805">
        <property type="entry name" value="Tra1_ring"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF1">
        <property type="entry name" value="TRANSFORMATION_TRANSCRIPTION DOMAIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02259">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20175">
        <property type="entry name" value="Tra1_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20206">
        <property type="entry name" value="Tra1_ring"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="FAT" evidence="5">
        <location>
            <begin position="2806"/>
            <end position="3410"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="3189"/>
            <end position="3222"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="4">
        <location>
            <begin position="3718"/>
            <end position="4046"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="166"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1098"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1165"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1283"/>
            <end position="1378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2141"/>
            <end position="2188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2750"/>
            <end position="2777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="3290"/>
            <end position="3337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="3415"/>
            <end position="3516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="166"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="193"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1165"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1289"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1314"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1334"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1362"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="2143"/>
            <end position="2154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="2159"/>
            <end position="2172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="3297"/>
            <end position="3325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="3417"/>
            <end position="3511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2071309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="4088" mass="460789" checksum="E884ED9325AF6A14" modified="2022-05-25" version="1">MNPAYFESYAAKITQLQKNNDFLPMLSLVTEIRDNIEVVHSVEYPSFLKHLFPVFEDILMKGNVQFNDGPEQKVRTIVLEILSKLPNNEVLRPHVTSLLHLSMKLLENDNEENALVCLRIITELHKVYRATLESEVQPFLNIVINFYTQLPITIEKTFNPTAVTTPTAGASAAAPTATSPPPATAGGEVQVGTPSNITTPPQSTTSPKPIAPATTSPPTATATTAANIIQPNKPPEPHMIPSNASFKLLTESPSVVVLLFKLYSQFFASNISKFIPLIVQTLGYQAPANAATVYASQYGDFIAAQVKTLSFLAYILVQYYEQIKGYQDIPQYVIQLLRHCPTHASSTRKELLIGLRHILNSGLRTRFLLYLDVLLDERVLLGTSKTSYESLRSMAYSSLADFIHHQRAELTIQQIGKVITIYSKHMHDPTNPVGIQVMSAKLIISLMEFIPRKDLDFKNRIIVYKSLESIINKFSSLKKQIPKLLEAAASGASNTNSSNTTNSDGSVNINDPIKDCRLLVKTMTSGLRNIFWAMTFRHQQLATAPPRVMSAVDESLLYIKLFKNTVKCFSIYGGANASLYEEKEILEHFTSSFIMLDQRIFQEVMSFLMPFLYQRSLEDQSLLLIPQGFLSLTGNHASKIFPEILTPFIMEKLKNTSGADKPDICLIRLIRLLFNGVTLVPDNDFTVQPILSTLILTILKLSSECKHADSMQYFILLKMIFRSCSKPELSKEIATILPGIFETINDLLIQSCYPQPIIHLLIELTLTIPVQVSFVLPSLHLLVRPFIMALESDSMELISIAFRMLEVIVDNITGDFLLNVFRENKFDFLQAIKKHLRPAPYFFGPHAIRILGKMAGKSRSTYALSPSTFNNTPTSDTEYNVFKLLLPVESGNTTASAPLPDLLNIPMDKAIESIKQILVFQQNNDVFYQKHAFSLLQHFLSLFLQPLESVDNQSLFKDLQQNIMQTNIPITLNSIHSNSYSNIEDNMSTLNINNNTTASSSTTPSTTPSISHTIEEYSQEIKNFKDLLFCLFISSFNDNLKDQAVPFLNQVIYHFVLYLSKRPYSSPVNSLKEIDPKIFIECLLDVIAIIPIDNTTPTAAPATSATTPATTTNSPPLNQSTVVPTPTPTPTAIANARDYISSIFDSIFECSDKIYGSCSIKKEDPLSPDDKKDLSTSQPPTTPPSPIFRYLVESLIHNCYSKNYTNKNASCIGIKYIITRCNVYWVQLYEPTLLRGLLFITEDCTYQGFQPIIDLASDVIHSLLKICTPELIPPPLPVVTKTEMEVDETATTTASTASTTTAMEGVEAKPAESTPVTTPQPTNPSTTPAPNPTSPTTATPATTTPGTTPATITTTTAAPTATPTPTPPPAPPKPFSTLSQLSENQRKLLNRVLDILFRSLMATQAHTRQIAQKMLLMISDDITHIPIHLLLSDLNITIQRILQKPLKILSTVAQTGVVEAITFCISQKPSLIPINADSVRILQDCLNTAGEDPTITIKANSPKIISQVSALRIASVEMIATAMTCPEFLAFDCVDFKNRIIKAFFRGVTARNKDLAAAAKKGLSNSITQQRLNRELLQLSLRPVLSNLTDLKNLSVPFLQGLSRLLELLSNCFNAALGEKLFEYLKIFEENGKLNAAANKFKDSEEVKISAAIIDVFHLLPPAIKLLDQTVNFVQRLEQNIGKEVTSPYREPLIRFLAKYPQKTLEIFMNPNIPFQPTHAQFFRLVLKQTELAKPIIDELANTYNVWLEPALNSQTPEIRYNALALMGIITKSCPRWLPEKTNILDILIQYWRSPKHYSNYHAPEITNQTLKETKILVKCFLQYCRAHQEETNLFFHMLIVLTLKGSMDFNFLRDFYQNELSKNSSNEQKKSIITTFLIFFKDQTIPSDIKVQAIQNLITPILNSYFALEPSQRTIEVHVFIQLSKQTMEPENDEMLLIEILQMQTILIKNMHTELIDCRKDWIKCALYHLKNEDLTCKQWAYVLACRFIEAYETPIKIVLQVYVSLLRAFQMESKNLVKQALDILLPCFEVRFPSDSKNIWVKWTKKIIVEEGHSVHQLVHILHLIVRHPLLFYPNRSQFIPHMVSALPKIALVSNITQENKKLAIDIAELIITWEKWRNNEHPLGGSSAGASVNMTTSTNTLSVSTPSTSIPPQTPINDASVTTPGQISTPIAGDGGSKDPNAGEDYKPPPSVIEHISVFLIRMLTSNLSTPPNAAAATASGDKSNSNEKCSELLKAALTIWPDVNIKFSLLEKPLNNPDPLVMSTALSILNIIVEHQVTAFIPANLQALQQSIFTSINNDDPKISALVCTFFKKVLATFPPLDTKNTPPEILNFYSTIGTHCESILINFEKNNNLSVLNLINVFKEENPNFLDTFIPHIIKVIVRLTRDYLLSLEADAPGATTAANGTPTKPAGATPLLASANPVNTNNAATKAKSLDNISGLCKCFDFLKSKLTFLNAEQKKLFLQSLQVLLERSNDIDLLTHIISIVSYLLFKDQNNLTSKDKMNILLKMNRLEQLNNTELISKYFNLIIEFYNDQSSSKQELSQLEQSFMMGLRFSDPVMRKSFFEILSKSIGTSLFQRLHYIIAVQQWDTLGNTYWIRHALDLLLGVLPCSKFVQLGSNNSKLPKLFNNSNTSAMNIDTPSTLNESLREHMDWLETNKNITFGQLNENLRELLFNDVYLVHNLWVHLFSSVWSLLSKEEHYKLSKSLNILLTKDYSQKISLVGLNPTPMKNITQSVCNNIFNQNNNTGKDGSGNSGTPPATPTTTQTITPSTLREPNIIKTFLDTISTCTPIPKIPVEIISFLGENYNCWFNAIKSIENNLIHDGGKLSDQNDVNWDYLSSLISQINEKDLLFGVYRKRFTCDETKLGLILEQFYMFQSSQEVYLSAMNKYSTVGAKPTSKVENILWEDHWIECAKRLNQWNIVFEFAKEKNLYDLLAESSWKMSSWQPNTIKKVMTQSETAIRKILQGYYLVMEKKYGEIDPIVVSANQLILKNWISLPENLIRSHTQNLVEMQQVVELQESVHILKEINNPNTNRMILVNYSKSIFNIWRERLPNKDEDLNIWYELMTWRQQVISLIGNILDTTPAAPAAGTAPESSNIVQPEIAWTMNKYSHIVRKHNIIEVCLNSLSKMFNLQIDVQEIFLNLKEQIKCYLQLPTHYDTGISIINSTKLDFFTYLQRSEFLYLKGEFLNRIGNYEDANQSFASAINLFENSAKSWIGWGQFCDNQFTNNKSLDFETKSQWAESALSCYIQGIKCDPKYAQRYIPRIFWLLYLNGSGEVPQQPPPNTQQQQQQQQQQQQQQQQQQQPPQAGSEQPQPQPTQLPNEPKKLTPAQSVFQAFLSSWNILPQWIWLNYMPQLITGASLTPNFPGYGFLCWQMIGKICFLYPSTSYYHFRKFVSEIKQANLNNPNNPNNPNNPNNPNNPNNPSNPSNPSNNPTTPTSTSVATPITPTSSNGVTPITPTSTAAPTPLPGNTTGSNAAATTTAAPSTTTGANPPTSSPFNYPPLKMADTLSNGLQQYHQCLIYEIDCMLDSFKMLASTVPAVYQFNATLSHLLNECLKIQHEEIPNNLIELFKSIYNHFFKEIPSIKYPKLPELINRYKVLFENDFVDILGDPTTTSSNEPFRMGIFIGKLVDWIQRVPDFELCTITYKDGSNNYYYENSIINIESICPLLVDFKPVILEVPTQLILSRDPNLDHHTKIDRIGLTAKMVKHSNGMLCPRIFIYGNNGKVYPFLIENEPIFVSQLQAPTRVPRRVERKLLLMSSLNAMLLKNRETRRRGIYLNDPATVVISPTMNLVQYIGGGEMTRFGDVASFASKSNTTATLLNQIQSYREKNVGQKQTKLQLYREVSKQFGDQHLIKYIQKNITCLDDQYEFKINFANHWGLHAILGYVLFSKFGQLSPNDIYFSRNNGYVYFNQWAFELANHSASFDVTPESTSVPFRLTQNLRSYFNPLFIEGNLTSSILATCVAMSELKDLLVNSINIYIFDEYMCMNSVTPLVPSEANQNRNIHYEFVDRTVKTVNQMLSLRINNITPNSQPDKTSFISPIVKKVNELIQLSTNSTNIAQMDKSSIPWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V533</accession>
    <name>A0A8J4V533_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Monalysin Pore-forming domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000021">
        <property type="protein sequence ID" value="KAF2077713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V533"/>
    <dbReference type="OrthoDB" id="2368263at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031157">
        <property type="term" value="P:regulation of aggregate size involved in sorocarp development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040927">
        <property type="entry name" value="PF_Monalysin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038768">
        <property type="entry name" value="SmlA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35884:SF1">
        <property type="entry name" value="MONALYSIN BETA BARREL PORE-FORMING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35884">
        <property type="entry name" value="SMALL AGGREGATE FORMATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18063">
        <property type="entry name" value="BB_PF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Monalysin Pore-forming" evidence="1">
        <location>
            <begin position="49"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="279" mass="32250" checksum="015FA9504D833B5D" modified="2022-05-25" version="1">MDIRGDHDKQEENVQGASVKDLFISNPNVTEEFLTILSNLPSYDMSKSPVVSSNEDVALNTKHKDIPHVFLMNRFTLFSGYKPVKYEVAAIPGETAVWARVNWNTRCKPVAVYTSFIDSKNVINLGTYSLEYRKGSSIVESWWRETTLSVGAQFKAYDASASVTTGYKYETTFSEEIVKGWSETLEKGDYMLYQNKILYAYEIRILDREDANYIARLINTAYDYDKSIPKAHVRGNRLFYFVDFNVNDPFFKRLSSNLYQTISFQKAVDYLMTDGWSRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V714</accession>
    <name>A0A8J4V714_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DDHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_005239</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000203">
        <property type="protein sequence ID" value="KAF2073454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V714"/>
    <dbReference type="OrthoDB" id="431378at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004177">
        <property type="entry name" value="DDHD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058055">
        <property type="entry name" value="PA-PLA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23509:SF10">
        <property type="entry name" value="LD21067P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23509">
        <property type="entry name" value="PA-PL1 PHOSPHOLIPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02862">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01127">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51043">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1013"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="domain" description="DDHD" evidence="4">
        <location>
            <begin position="420"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="91"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="515"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="592"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="658"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="251"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="22"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="135"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="515"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="598"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="658"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="698"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="710"/>
            <end position="736"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2073454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1042" mass="116416" checksum="75C6F9927A089ACE" modified="2022-05-25" version="1">MTEQNTISMSSNGIIENMISYQQQQQQQQQESSTPTTNIDTNSNSSNAVDIDSLSISSIKRKIDAIDIDNISQITDNDDVGDKVKVELIDTNTNNTATTPPTPTHSNNKQRKDKEETIVIDDENTFELKEINHQNNSNGEGNNNNNNSNNNIDDKISIDLENDNDLDALEQTLDSFLNEQQSNNDINDPLTSLSNEEISKLSNLSPPLVSPLTALINNSNGFSSGAEQLDHNTPPPNPYEIDPVLKKKYVEEEMAQEKLKQQEKIEKEKQSVVDHIVFIVHGMGAQLGDSRVITLEQNVNLLKKNFNQFQKEDQQIKVDFQIIEWHSKLRNTADLNENIEKISPSGAKKVRDFVNETLLDVLLYMSPLYNQEILKEVSCQINNVYETYLNTHQNFKGHVSIFAHSLGSVIIWDILSKGLIKFKVENVFGMGSPVGIFLTLKGINIGQLDIKSELPNCKNWYNIFSKTDPVAYRIEPFIDSNYLNYEPCNIEVQPEKKGFQKFTGFFSFKSNTATTSSTKLTNTNSSSTTTTTTTEMLSSSPSTTTTTTNNTNPNNTNPSTTTTNTTSNDVEMVDKTNQELVENVNIKKFKIHPLEPDSPSTSNSPIIINGSNSNNNNSNGGIVTDSIINNSPSSTTSYLSGSLSGSLKSKFFMSFMSSNSTSVSKSPTQQQPLPTTTITTPPPSTTSTGPTIQKEPISKQSLPLNAPPQSSLLSEAVASSNSSSSASTTIPSSSPPKNIQENIMLSKELMVDFEKGEKRYDYCMEEQSSLWMNAMPAYVGSIFSHLCYWDSGVVALFIMKKLAFSSKSTNNSSNNNNNTTATTTANGANKDKDYIYSKRNMKEIGDLNLGVDLDEIDKLLEGRVTNINDVYKSQEFKQMIDAWKSQKNQLRASLIEFWNDYQEKTEFVSWWNKLNNQYKTALLLTSIEELKQDLDNYEEYSGIACPELLDTDTLLKSVDSEYDYSAQPNVPAISTVLFRMINQAVDSKENDSSNFTGLNRMKLHYSDLEDPQFVVQSMLVVRSCILLGFFVNIVLLYRSQDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7F9</accession>
    <name>A0A8J4V7F9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10">Peroxisomal membrane protein PEX14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 10">Peroxin-14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_004773</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the PEX13-PEX14 docking complex, a translocon channel that specifically mediates the import of peroxisomal cargo proteins bound to PEX5 receptor. The PEX13-PEX14 docking complex forms a large import pore which can be opened to a diameter of about 9 nm. Mechanistically, PEX5 receptor along with cargo proteins associates with the PEX14 subunit of the PEX13-PEX14 docking complex in the cytosol, leading to the insertion of the receptor into the organelle membrane with the concomitant translocation of the cargo into the peroxisome matrix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9 10">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the peroxin-14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000175">
        <property type="protein sequence ID" value="KAF2073919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7F9"/>
    <dbReference type="OrthoDB" id="441517at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040554">
        <property type="entry name" value="KPWE_PEX14_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025655">
        <property type="entry name" value="PEX14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006785">
        <property type="entry name" value="Pex14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23058">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23058:SF0">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17733">
        <property type="entry name" value="KPWE_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04695">
        <property type="entry name" value="Pex14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="3 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peroxisome membrane anchor protein Pex14p N-terminal" evidence="13">
        <location>
            <begin position="129"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Peroxisomal membrane protein PEX14-like KPWE" evidence="14">
        <location>
            <begin position="609"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="76"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="173"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="407"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="459"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="622"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="246"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="323"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="11"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="34"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="178"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="212"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="408"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="463"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="494"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="523"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="555"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="572"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="585"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="630"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="658"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="688"/>
            <end position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2073919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="698" mass="76442" checksum="AF87617437162BB2" modified="2022-05-25" version="1">MSDIQDSGLLSSDNTINNNTTNSSMDTFDQQQSNTVAAPAAVATNNQEETDYADHEELTRQRIQKRKEEAKRIIEERKKKEAQQQQKGNSAEQEIYEDDGNQDVDPQPQQSTGRIAAVKPAAPPKPNVREDMVRRAVSFLNNPSVKNTALGRKVAYLEKKELTSEEIKEALRRVETGNSSSPTSNTSSSAALNTVRGGKRIPPVITPGGGTLNTTSNNSGTNNNNQLVSSNNNNNNNNNQYYQQIQQQAQQQQQYYQQQIQSYQKRIDDDNQRIATLINASHRFSWGNLLFSVTALVGAASGVAYLTSNYIVPFFSKNKKTRESETDKKISDLQEQLSKLQSTILSQTSDFRDTVHSLKSIIESQNQTFLATQQQQLQQQKSTSSSLNSSAEILEMKKELKNLANLINSNNSNSNNNNNSNGYVSSYNNNNNNSNNNGYKPINTFPPTINSVKASNESEFRIPPNNFSSTSSSSNTTSSTPTTPTKPSRPIPSVVTSNNYSNLAWQPPTDQPPAIPSWQQKPTSTTTTTTTSTISTTSVPIVNGNSNSSSGSSSPTKQLNSSTGFKMEPSESKFTPSLPASNFNSETTDSSSSSPTTTTTSNGKVEEAPYSTDFLDVINQLKSGKTPPGIRDDIDDKPIENGTVNKGAKQAPKKPWEKNNTTLAPITNKLSSDEIQSLSTDNIDNDVVVDHDEKPSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V851</accession>
    <name>A0A8J4V851_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L7a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_004049</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000136">
        <property type="protein sequence ID" value="KAF2074654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V851"/>
    <dbReference type="OrthoDB" id="29563at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001921">
        <property type="entry name" value="Ribosomal_eL8_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00882">
        <property type="entry name" value="RIBOSOMALL7A"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="5">
        <location>
            <begin position="137"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2074654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="280" mass="31192" checksum="D6B92C319B72168F" modified="2022-05-25" version="1">MSKNTQAKPAAKAAAKPATTAVVKKKTEKKPKTGTIFNALYTKKVKNFGTGFGVLPKRDLTHFTHWPRYVRIQRQRRVLVRRLKVPPTINQFSRVFDKNTAISLFKLLDKYRPEEQSVKKARLLEAAKARAAAPKGEKVAKAEKPVSFLKHGIDIVTKLVEKKKAKLVVIAHDVDPVEIVIWLPALCRRMDVPYCIVKSKSRLGQLVNLRGTSCVALTGVNNTDINDLSLLVEAAKQMFNNNSEHRKQWGGNTLSGPTRAIIAKRVKAANKETAAKAKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8W2</accession>
    <name>A0A8J4V8W2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000487</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000009">
        <property type="protein sequence ID" value="KAF2078197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8W2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="410" mass="46726" checksum="1DA38E8D6A8B45B4" modified="2022-05-25" version="1">MDLFFQVWRNNYIRSHVRNQTIDGCRIDISFDYLERNHRFLSMLDTKLFDIDICVHLSNSGDDQRFQDSPLRHLATSLQGVYDDRKHIDHLPRLKSFGTFENEVGGVIDFSYLPDTVTEVSIALLFAKSIINFPKHLKKCYMLDNGISVHTPVGKDFFPASLESVVFSEYYRGIITHLPENIQEIEMDSGIKMFTNLDKVLVGKSVKEKERIFRKSMVTTYDKNQLASLRALPWLTNVRFPHTSDFKFEENIMPSHIKRLSILSSQPNQIPLGSLNQGLVYLDAPQFSSPLTNGLLPSTLQEINLNSFNYPLDFLPTGLRILKLAQFNQVISIGSLPTSLTHLEIPKFSGSILKDTLPSSLTFLSIGHNNSHTQVYHGGIKKLDVDFQTILKDQYSTLCLCQSLVSHLSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V9L8</accession>
    <name>A0A8J4V9L8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Roadblock/LAMTOR2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002422</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GAMAD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000067">
        <property type="protein sequence ID" value="KAF2076244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V9L8"/>
    <dbReference type="OrthoDB" id="271745at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032008">
        <property type="term" value="P:positive regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.30:FF:000004">
        <property type="entry name" value="ragulator complex protein LAMTOR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037587">
        <property type="entry name" value="LAMTOR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13323">
        <property type="entry name" value="LATE ENDOSOMAL/LYSOSOMAL MP1 INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03259">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103196">
        <property type="entry name" value="Roadblock/LC7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Roadblock/LAMTOR2" evidence="2">
        <location>
            <begin position="17"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="124" mass="13842" checksum="41CA97FD4C43181B" modified="2022-05-25" version="1">MLRSKILPQVLTQAISADIKGVLLMKEDGSLIACSESQNNNSTEHNTSKVISAITSNIWTSYNRTKDLQYQLIDCEEGKFVVTKVASLLLCVYSDATIEFGLLKSKAQKLREYLQEPLSKVETI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V9Y8</accession>
    <name>A0A8J4V9Y8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000055">
        <property type="protein sequence ID" value="KAF2076604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V9Y8"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42264">
        <property type="entry name" value="EPHRIN_REC_LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42264:SF3">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1027" mass="119873" checksum="30EE9C105C748B3E" modified="2022-05-25" version="1">MIRNLINSKRICPLTKPNALITNNISSTSNRSKLLHKNSNSHSNNVSSNRSNLNTLFLKSNNYIKKQQYCTSTTTISSSLNNNNSNDDSITYQFDFESLQEKNALVDYFKDKLKNQPESGSKELKYYLNNINNFNDNNITELFKVYTSKNESSYDIDILNLFLESFIKSNQNQHMINSVLGILSTSFNLAYNATTYSLLLEYYLNSQVKDKNELQEFVSKIIESKVKFNDTMDYMLVEYYLGNMALKKAVKFFNKTHTYNDRFTALQICEIIEFLILENEFNNSLTFLQQAIKTHPKFLSTHEFLYVLNLFALNNRSDDCFSIINFIDHYFKSIENSNNSIGNNDSNNSNNSIEDINNYLDLMYNNLIKFYSNQNNRTMSVLMLEKMIQNNIQTIDSFKPFIKLCINNNDEKLLLSYFDQLDIYKIGLTPDLIYHAMTYFDSVTGNEDQVSRLAKELLKNPDYYALVFIYAEKVGNSSLVTFLTRNLTTTAKDFADRMTNYMILYYLLMDDFESSLQWYTSRQVTFHIRPTFDTYYYFIRYSMVSNEQGTASYWQQRADELVASDHRLTQEKIDEIKSSINYKFDKEYKTDPIQSIESFYDEFNLTVNPKLLKFNHELLDYIFNKMQQQPTTTQRNPTSSIQLNENDKLLDNYMAKKDYVNLEKCIKRLFKMGYCPPKGLLAKVLDCIYINNSKNNSSVFLKFFYSIPIEERPLHFISGFYSCLLKFNINAGINLLSKKDFILFSINSDIWNSIMISLVYANLKLAISLSPKVIFKNKIYDLTYIPLLAHELNQILFKYFLKKEKLHSEGHNNSTNILISKLKVKYHKLNNNSNSNSKTSNNNLPNTIDSSNIDYRQDILDKYSQIIIDNIDGLMYILKGVGDVPSFNILNTCIMALLANNQYREILKLVNEQPEVNRLSCILTIRAIREVFKQDQKKTQIASNAFIAKNRVNLQISKEEFEYLMNSQSDLSFSSQFFNQFITKLLDKEMETLIYRQSILNSKPPPLLTEGSYDFVVSMIEFDITEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VAI3</accession>
    <name>A0A8J4VAI3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ABC transporter B family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_001495</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000036">
        <property type="protein sequence ID" value="KAF2077174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VAI3"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18582">
        <property type="entry name" value="ABC_6TM_ATM1_ABCB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03253">
        <property type="entry name" value="ABCC_ATM1_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000004">
        <property type="entry name" value="ATP-binding cassette sub-family B member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000186">
        <property type="entry name" value="ATP-binding cassette sub-family B member 7, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221:SF402">
        <property type="entry name" value="IRON-SULFUR CLUSTERS TRANSPORTER ABCB7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="273"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="387"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="421"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="161"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="481"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="108"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2077174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="724" mass="81375" checksum="9162C6BB4A0F3464" modified="2022-05-25" version="1">MYRIVNGSANNTFALSNLLKNTIKSSTKCYFSSSGGAASSLAKQHQHQQQYKEKQQRQQQNIFKLSNLAKSSLPSSSISTSSLLNDIKKEEPFIKFYSSLNKNKTTTTNTTIEQKDNSKQPIKQDDKGKKKEQENSTMSMLKTVAKYLWPKDNNDSKMRIVASVILLFGSKILTVQVPFIFKDIVDHLTSTGTPMVFTLPLGLLMGYGAVRIASMGFQELRSTVFSKVAHDAIRDVSTNTFRRLHQLDLSFHLSRQTGSLSRIIDRGSRAINFLLNSMLFHVVPTAFEIGLVSALMYTQLGWEFSALSLGTIAAYTVFTVKVTQWRTQFRVKMNSMDNEASNKMMDSLINFETVKYFNNEKLEVERYHNYLKEYDRASLKTTSSLSLLNFGQSFIFSLAMTAIMIMTAQGIVNGTMTVGDLVLVNGLLFQISLPLNFLGTVYREIKQSLVDMDHLFSLLNLKPKIHDKEQSKPLELKNGTIVFKDISFAYNDSKQVLNNVSFECEGGKRVALVGTSGSGKSTILRLLYRFYDVNSGSIQIDGQDIRDVTLDSMRKNIGVVPQDTVLFNDTIYYNIAYGNPSATKEQVEHAAKLAHIHDVILNMKNGYDTVVGERGLKLSGGEKQRVSIARAILKNSPIIFYDEATSALDTATEQVIMHALRELFKNRTTIMIAHRLSTVVDADEIIVLGHGGVILERGNHHQLLDIENGKYRSMWLAQQFENEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VB71</accession>
    <name>A0A8J4VB71_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000772</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000016">
        <property type="protein sequence ID" value="KAF2077894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VB71"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="265" mass="31287" checksum="2556748A834F76CB" modified="2022-05-25" version="1">MLAGRGSSNNNNGNGNGNGLDKDKQPIKSILEDKKISARESEQQLEKIREKVKKELKDKSTVDLKEKKELLKKQYFQLANKILPEHLDMFCELEKTWNFTTYKSNPEIYMEMCREHGKYSTEFNIPIADIKNRNQLCGFLFKKLTDLQFNIILFNELLGNSQASFEEGDVYAKLVSSYVLVYFGYILRNLSTYFREVQTRDARHIEPHKLVKYNLPDLNHLDLKYKDHDNYYLIQIVEKTKSHLIELLSFIYKNDTNMKFIFNQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VBV3</accession>
    <name>A0A8J4VBV3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_000192</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000003">
        <property type="protein sequence ID" value="KAF2078569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VBV3"/>
    <dbReference type="OrthoDB" id="18717at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000060">
        <property type="entry name" value="Serine/threonine-protein kinase isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF298">
        <property type="entry name" value="MIXED LINEAGE KINASE DOMAIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="499"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="71"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="355"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="402"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="104"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="402"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="426"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="526"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2078569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="752" mass="85676" checksum="C755023135E13CC5" modified="2022-05-25" version="1">MKGGGYYHQQNYNGYYNPSTSPLVSPSPMVAMVGGGGHQMERMGFPINNNINNNINNMNNGAYGHQQHIPQQQHQQQHHIQQQQHHIQQQQHFGGLSPNMNHIQQQQQQQQQQLNNKLNYLRQHQRNSSFDEFSLTTESLGMGGFKELTWEEIYLCYHHFQQMDDGHGVLKSFSQLVKYISSLYQPDTPPRTQFLLAIGLLYPNAIPQQQSPTMILSLTLKQLIEAYSYTKSMVYNGNNNRNTTPPILNQINLTVGQYQYLNIRKFTPQQACAIIDVFNSVKDKSGGVPTIKLSLFGADPSMLPATTLPFHEFVQYIGLGIIPFEHLKFPQQQSSSPPIEGQQIFITEIDDLDNNINNNNNNNNNNNNNNNNNRQPVVYFDNIDDNFNNNYLNHINMNNYLNINNNQNQNNNQNNSPLNPTKHNHNNSFNHNNNNNNNINNNQNNNNNNNNNNNNNQNNNNNNQNINQLQPPQQQQLKTSGGVNKPEYHSDIEISFSELSIISKLGEGTFGVVYKGIWRGSSVAIKQLKINEHVNNQVLEEFRKELTILSRLRHPNIVLLMAACTSPPNLCFVTEFLPGGSLYDALHSKKIKMNMQLYKKLAVQIAQGMNYLHLSGVIHRDIKSLNLLLDENMNVKICDFGLSKLKSKSTEMTKSIGSPIWMSPELLMGEDYTEKVDVYAFGIILWELGTGELPYAGLDSVQLALAVTTKSLRPPIPSVWPQNLVNLIQSCWHQDPHMRPNFSEILNQLEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VBW8</accession>
    <name>A0A8J4VBW8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome P450 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_000114</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000002">
        <property type="protein sequence ID" value="KAF2078614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VBW8"/>
    <dbReference type="OrthoDB" id="15258at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20617">
        <property type="entry name" value="CYP1_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24303:SF31">
        <property type="entry name" value="CYTOCHROME P450 307A1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24303">
        <property type="entry name" value="HEME-BINDING MONOOXYGENASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0349">Heme</keyword>
    <keyword evidence="9 12" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2078614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="478" mass="55380" checksum="C6AFAA5772F46180" modified="2022-05-25" version="1">MLISIVLYLLLGYIVWSFLEKNVIGIRNKVKGPLSLPIIGNLHQFASPHFTFDQMANQYGSVFRVWMGDFYALIINDIDLYKGIYVKNNKLFINRPLSPGFQRISGDFKNIIHSPNPVWGENKDLVKHNFTNKALVGPTKPIFEKHMKILVDKMDEAYDQSKLFDTKYYIERCHMNMVLDFFTSSHVGYEHDSDEWVAKFNDSLIGVVEDARYGDILSSVGFLSPLYKRKLAGKRPSNQNIIDYLDVELKKHKETYNPELKRDIMDALITSFDESKHDRINYIIHDFFLAGVESQVCCCEWFFIYLSTRPEIQNKAYEELKSVFGDSKVVDYSMKNKTPYVNSIIKELLRIKTPLPLSIPRIAEEDVTINGIFIPAHTLIIPNMYHIHRNEKYWSKPTEFDPERFMVENETSLPYIPYGLGERGCIGLNIANSCLYHVIATVLLNYSFSSKTLLSLEEQSDLVIHPKNRSINVILKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJ47</accession>
    <name>A0A8J4PJ47_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_010564</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001198">
        <property type="protein sequence ID" value="KAF2068110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJ47"/>
    <dbReference type="OrthoDB" id="24449at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052014">
        <property type="entry name" value="Dictyostelium_Tiger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31341">
        <property type="entry name" value="IPT/TIG DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035252353" description="EGF-like domain-containing protein" evidence="3">
        <location>
            <begin position="26"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="517"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="572" mass="60787" checksum="05588A8E550C2206" modified="2022-05-25" version="1" fragment="single">MKSFYFILFCFIFSSQQLFQNVVEAQAPIILSIIAQDNALVIGIDDSGTGQNIEQVIAGSFSCAITQVEADLVVCNPPREFYKLKSFTVQVTNILGTSSPFSYTFQNGIDSFSSSNNIVLFIGQMRDTMDTILLNGITPSPSSFSSITSSYLQFVYTPSIRCGLAYVKYNALRVSNDILFCPIPAILDFSFLNNQIVLTGKYLEPTSYDNLEIYLENSDGSKNTCSSTPVANGNYNIICLANTAYRSFYFNAVYVNTNQKTSIAFNNNPLINGVEPTIHGVPGLVTIRGLNLNSAGGKTVVTIGGEACAFQSSSANTIECSFQSVVADPVPLQIMVTLDNAYIATADIYKYKQGQCPTSNGADCSGHGTCKPDFTCGCSTNWLTDDCSVIYPQLTSISSTYFGEPAIVTIVGSSFASSNLIVTIGGSSCSNPSVIDENTITCTFQSDVAQNAQLDPLVVFLSIDTKNTAQKALFSYATKSSSSSSSSSSSSGVDNCPVGSNGKICSGHGLCKDSIACVCDKGWGSTVDCSTISPNIISSTSTKFGSPSNVTISGDNFAPVDLKVKIGGLLCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJT9</accession>
    <name>A0A8J4PJT9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010449</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001120">
        <property type="protein sequence ID" value="KAF2068227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJT9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="41"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="101" mass="11356" checksum="CB17CD5C73179787" modified="2022-05-25" version="1" fragment="single">MDTIKVYQSNYTNNIQAKITEATGFIKTLQNNITQSENEIIDNVNTIIDKISKLVDDAEKENQEYQDEIKQLKSNLIGREIVSGISTICAGISIFIPEVAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PK51</accession>
    <name>A0A8J4PK51_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010251</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000996">
        <property type="protein sequence ID" value="KAF2068422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PK51"/>
    <dbReference type="OrthoDB" id="23204at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="723"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="725"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="735"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="791" mass="86793" checksum="147D9DA8C1F35B12" modified="2022-05-25" version="1" fragment="single">VNYQVDQCSPSKVISLPTLTYESDQIEETIEIITYPSCNYAFGNVSYTVRDVVNNISSTSYSSFRASQEFTLDTQLPQCSQIFYHDWTSPPIQKYYNNFPGTIDVIKKASCKYSSDASIFITAPSPIQNIVVNGVSIYQTPNQPGLYQGLPYGVYDLVIIQCSYHYVSVSIGTDNDWEFPAVVTPVTGDCSSSNGKITYENNGIFTSVNDLSDFTAVSSGYHLINFILSNGTCGGSAEIYVPTQSNFVVESTVIAQPLCQDSKDGVVKVTIKDTNGLDYLPTSIQVDAFTTTPNNTFAVKAGNYNMLVKRDSCSWFHKVSVEANEPEFAFKKVFDSIEYCSQKTFVTITPSSSNVLIDKVSSEYGFTPYSNNAFSYYPETFRKTTQMFITYNKVCLKVLRVEESSDFYKNIQWPTITVPSVDCTDPSVQNSVAQISNTPNMIMLPSSDLFYPTYTFAPNSLGFVDMKTQCSNVVEINNLNQGSVAKTITKPTCPGSIDGKIQIDFDPQSNIHHVFSSDFRYLPVESGSELNTFNNITNSEYHLIRSFRNNPFCSVVENVNIVVDEPTVSLSSVGYCDASDTPISDVGVVTNTLSITTTNVTYNLDGVVSSDPVFKGLSVGEYNSTVTIYNSVCRRLIQSNSVSVAQLPSVSVSVDVSTCMKAVINPTVTTIQHQYTIKDSTDVVHSSISMGSFTFTATKKDTYSITVSDSTCSYKTTFSIAECPTQPPTNPPTQSPTNAPTSSSSSPTTGSSSSTEPMPSSSFKLSPIYSPSLSLSLYLLHYYLNKPLSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLG0</accession>
    <name>A0A8J4PLG0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_008603</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CCC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000546">
        <property type="protein sequence ID" value="KAF2070077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLG0"/>
    <dbReference type="OrthoDB" id="73465at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02434">
        <property type="entry name" value="Nodulin-21_like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008217">
        <property type="entry name" value="Ccc1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31851">
        <property type="entry name" value="FE(2+)/MN(2+) TRANSPORTER PCL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01988">
        <property type="entry name" value="VIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="293"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2070077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="326" mass="36096" checksum="C1AE96DB4328BE5D" modified="2022-05-25" version="1">MEPIELDDREDEAVLSGSQTVNKEYMDEPLIDKSSDIENDDDNQTTNGDQDENDNKSNVKVIKNKHLNKVFDKIKSIDKEDLKFDHSKYLKSIIYGGMDGLVSIFVSIAVVASGDAKITVLLVIVLSKLIAGAISMGMGDYLGTQADVDYAKGERKREAWELEYYPEGEKKEMVEIYTSRGVPYDVASEVVEILSKNPKGFIDIMMVEELGITPDAETEVAWKNGMVNFLSFLVFGIIPLLPYLFFMIIAASAGLPNKENIGTFITVIILSVVTLFGMGVFKAKETDTVWWKNGISTVLFGCLGALIGFLTVEILKLIFPDIDLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLJ7</accession>
    <name>A0A8J4PLJ7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Leucine-rich repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_009477</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the ANP32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000719">
        <property type="protein sequence ID" value="KAF2069203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLJ7"/>
    <dbReference type="OrthoDB" id="2160613at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045081">
        <property type="entry name" value="AN32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11375">
        <property type="entry name" value="ACIDIC LEUCINE-RICH NUCLEAR PHOSPHOPROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11375:SF0">
        <property type="entry name" value="ACIDIC LEUCINE-RICH NUCLEAR PHOSPHOPROTEIN 32 FAMILY MEMBER A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="173"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="200"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="246"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="262"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="286"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="351"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="364"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="393"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="443"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2069203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="483" mass="53666" checksum="64147904F8F97897" modified="2022-05-25" version="1">MDQHIQNAIGNNDPSTMLQLVLDQKRLAKLSDLEIFQELEILSLNGCSLQSIDSLPILPKLRKLQLNDNRISGGLGVLGNLLNLESLSLGGNQIKSIEDLEPLNKLSSLKYLDLFACPVTLSPNYSKTIFSKIKSLIALDGYDKNGKEVEMDDEEDEDDDDDEDDNSEFDDIIEDEEDGNNNNNNGHRRPKSNGKSHSNDEEEEEEEDSDQDESDEEDEEDESDVGGGRHTSDKTTTKSPPNSKPNTTLTTNNNNNNNNNESDQDESDSEEDESEDTSSYKYSMATKTPTTTTTTTTTTTTSTSSSSTIKSNYQDSDDYSSGSSPPSSPSSSDSDDNSVKNNSSKSVKKSTTAYDMLNQSYHSEDDEDYQSSSDDDDDSSGGSGGGGGKFNQDQEDDDDDSIDTDEEQEEEDDYDQEEEDKLVEEIKRQRNQGIYNNNQKDESEAEVDEDDSDDEDEDEDEKIYQKRKNSTSDLPLPKKRAIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLL9</accession>
    <name>A0A8J4PLL9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008436</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000520">
        <property type="protein sequence ID" value="KAF2070240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLL9"/>
    <dbReference type="OrthoDB" id="10412622at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035249468" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="73" mass="8242" checksum="14F0E08064E20AD5" modified="2022-05-25" version="1">MSFAKNILYVTLILCVIFALTNAVDVDNGVNSNVGENLMATKKYCSKLSKLDCILNELTKFTCIWLNNRCEVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLW5</accession>
    <name>A0A8J4PLW5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_009312</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000677">
        <property type="protein sequence ID" value="KAF2069368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLW5"/>
    <dbReference type="OrthoDB" id="552049at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051964">
        <property type="entry name" value="Chaperone_stress_response"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054076">
        <property type="entry name" value="ZUO1-like_ZHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44029">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44029:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21884">
        <property type="entry name" value="ZUO1-like_ZHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="7"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="288"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="582"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="302"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="603"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="219"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="336"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="354"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="364"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="389"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="408"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="431"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="441"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="470"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="565"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2069368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="628" mass="73924" checksum="3A5074E674CC1CE6" modified="2022-05-25" version="1">MTTKQRCYYEVLGVEKVAKADEIKISYRKLALLWHPDKNQHQITLAEERFKEINNAYQILSDPNERKWYDDHRDSILRGGRGGGDDDSDDEDDINLWAYFSPNVYSSYNDNDENGFYMVYDRIFEIIHQAEYDADNDVSSPPCFGTSKTPIAEVLRFYNHWKEFCTRKKFSYVDKYNMRDAPNRYVKRLMEKENQRDRSRAKQTYNEKVRHLAKFVYNRDRRIAEYQQQQAELEEKRQKEEEIRKQKAEEEHREAMRKFKEEKQAEYERDRAKGVYDDVPQEEDIQEFYCIACEKSFKSDRQLQNHENSNKHKQQLAKLRAAVAMENEMFDGDDIGTGTTPTTNSDMDDDDDNNNNSFSSSSKSKSKKKKKKSKVINHYDSDNSDNDDHESKQESTHSDYRKENVHGLKTKKNNNNKKKSKQQQQKEEEEERKRREKELQEQEEQEEEEDDFSDEDDMLVNSLLRKSNIVDDDDDDEDEDDEDHEKDIQDEDEDEDEDDEEDKEEEGDDDNDDGDDDQDSESEGEKEEKKDTKKNTKKPTPTPQPTNNNNKKSSSKDKKSKKKSTTSTTTTASSTTGSDEVLYCNVCKQGYPSRNKLFQHIKESKHAQAIPSYPINNSSSSTSKKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLZ9</accession>
    <name>A0A8J4PLZ9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF5898 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009270</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000667">
        <property type="protein sequence ID" value="KAF2069413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="23627at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045417">
        <property type="entry name" value="DUF5898"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34871">
        <property type="entry name" value="DUF5898 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34871:SF1">
        <property type="entry name" value="DUF5898 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19250">
        <property type="entry name" value="DUF5898"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5898" evidence="2">
        <location>
            <begin position="350"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="535" mass="62130" checksum="D21C6A174699C54F" modified="2022-05-25" version="1">MEEDLKEKIKQLEQDLKEAQEKNKAMKEEIMAVEKAMEEKMKSFQKEKESKMIKNLFVKIPTGQRSTINHTPSSSKHRPCKFEKSPFSLLENFSELIAKNGEKESSLLVNTKKNCFKFENESVLSIYVRYLLEDTIKILGLEDQISIDYQKPVFDLLPECWFLKFGKEFIGSIEVKVSDDSFDNPKIVGQVFDYAMGSAFHFSKLESFCILTTFNRAAVYFTQQSKDLAQEKKDLRKVHLLKDPMVSEKFYKTVKSKDRFPSETRVLYRSKIYDNTEPENFLNLFCSAIVKMKSSQHDPTKEHSRMRAAVVLTEKGKGFETKEFKIKQGFTELKTLDQIFLKKLYMVKGLGGGGDGFCSLAYTGNGYPVVIKVLNAGGTRRDLEKEQLMWYEMWGIQTEICTYVDEPHLVMPYLKVVSETDFGGDKKEIFFESVKKSCKDITQKGFKHLDLRLRHIAKLKNQKETNYVFIDLSRVESGITDPKKAMEEMMNDLEKDFDDYMKNKSKKSTINQFFVDRCLDRFKTSSVKQNTPEFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM43</accession>
    <name>A0A8J4PM43_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009974</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000869">
        <property type="protein sequence ID" value="KAF2068706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM43"/>
    <dbReference type="OrthoDB" id="18713at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="1">
        <location>
            <begin position="6"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="103" mass="11424" checksum="6F88F2B4C84510FA" modified="2022-05-25" version="1">MPMINMNIYCPSTNQTIGIEGLTEGSSIKDLKRIIYERTGTPPEHQTIQIDNKKIPKRYDSKKWEKLGINDKTNVQMIYAMDGGCGCGCDICGCGCDACCQII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMD1</accession>
    <name>A0A8J4PMD1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TRAF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_009556</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probable adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000740">
        <property type="protein sequence ID" value="KAF2069124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMD1"/>
    <dbReference type="OrthoDB" id="26354at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001293">
        <property type="entry name" value="Znf_TRAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131">
        <property type="entry name" value="TNF RECEPTOR ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131:SF69">
        <property type="entry name" value="TNF RECEPTOR-ASSOCIATED FACTOR FAMILY PROTEIN DDB_G0273435_DDB_G0273505"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02176">
        <property type="entry name" value="zf-TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50145">
        <property type="entry name" value="ZF_TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TRAF-type" evidence="9">
        <location>
            <begin position="164"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TRAF-type" evidence="8">
        <location>
            <begin position="164"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2069124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="434" mass="49985" checksum="7F342631F2349F9A" modified="2022-05-25" version="1">MIEVPLVYDLESILQTDNTDGYECAICASIKSQQYQCTRGHLYCLDCWKQSIGKESQGKCAECRLPITLDTISRNLFYEKTFQNETVSCPYYNHWQSNNTTTTTTTTKTTITTKKEEVIQDKCKDMKYKQLVDLHYKECIYRLVKCAGEGTTCKVMNFKDYQQHAATCPYQVFLCKECGKEIYSKDTAGHIKECIEKVVNCKYCNTALEQHKMLDHVSNQCPEFEGMWIFYEISAFERIKRKDIESKITKSQPRSPKNPFSPTKVFKGPLPFSNLFQFEQMIGVISEHLEIGKATATNRIYWEVAIDFVQYKGSFHIDVKDPKVQISITSSPSPLPSSWKLGVSFFNTQYQDVFTAVYTSNQFKSIIIDKKSFKFNNNNVPYRSTAGIGGEQMVSITAMDHYQSLSFEELRLQDLINTHKHLMNLTLLINKTPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMV4</accession>
    <name>A0A8J4PMV4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007849</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000442">
        <property type="protein sequence ID" value="KAF2070825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMV4"/>
    <dbReference type="OrthoDB" id="10566412at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="133" mass="15159" checksum="2C2E9C5632D1CC8B" modified="2022-05-25" version="1">MCPIKIKPENLQFDKKKQNNKVEFMITNDGFDLVDLEVNLLSNDSDISKKITIPDIRGSAIICEQNKPAIIPINPQEHMLFYVIYKGENDEKSCHSQSTIPTNNLGKSNTEIELKYTDETGHRNRISVPINVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMV8</accession>
    <name>A0A8J4PMV8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008515</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000532">
        <property type="protein sequence ID" value="KAF2070158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMV8"/>
    <dbReference type="OrthoDB" id="21006at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025224">
        <property type="entry name" value="CCAR1/CCAR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025954">
        <property type="entry name" value="DBC1/CARP1_inactive_NUDIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045353">
        <property type="entry name" value="LAIKA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14304">
        <property type="entry name" value="CELL DIVISION CYCLE AND APOPTOSIS REGULATOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14304:SF11">
        <property type="entry name" value="SAP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14443">
        <property type="entry name" value="DBC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19256">
        <property type="entry name" value="LAIKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01122">
        <property type="entry name" value="DBC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="1051"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="261"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="680"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="882"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="91"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="114"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="261"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="293"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="317"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="360"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="395"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="411"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="707"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="768"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="882"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="917"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="948"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="975"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1008"/>
            <end position="1038"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1122" mass="123727" checksum="AFEA22A1782A7D73" modified="2022-05-25" version="1">MDWQQQQQQQGYSQGQMYNNNNNNNYNNNNNMGNNNNNNMNQRYGSNQSNYNNNNMGNSNNQGYSMNNNNNQYNNQGNFNNNTSGGGNKYQNQNQNQNQNNQNFNSFMGGGVNNIGYNNNSNNNSNSQVFDPRMSSRQSSPNQQSMGNMGMNMAGGSNMNMGGNNNMNNNTSNQMNMSNQMGNQNQMSRQGGNMNNQMMSNNNMSNQMGNNQMPLMGNQMGNNNMNMNNMNNMNNMNMGGNNNNMNSNMNNMNQMNMNQMNTNNNNMSTNMGNQMNMMSNQDYQQPQQPPPQQQQQQQQQQQQSHQTKGYNKFNKDYSNSSSNTNSNYNSNSKYSSGGGRMNKPYKGGKGDSGHGSGYYSRKDESSGSSYKDKSFHKEKKGDYSSSKKGVGGASSGSSSTTTSTNAGDDQSSSSYNLNSRRSSEPSSNNSSLDRDKKYVVRIPSHHFNQENYDYLSLKKNFPKLQISPDFTMLVSSWVETVNNSNGNGGMGGDGNGTDTNADEDNTGTLNYYQEIPFVIDSKDFGITKVDVNDTCFSSKYRSTIPSFTSDNGNIKYNSKVMLFTYYPSSSSGGGGEEVSFLKKLKFLVSKEDKEINCVGGVWDPLLDGNDINDPQTLINTAIRTTKEYTQLDLSGCKKWTKFLEIHYYRPPTDTSSFYQEVTVIYVPDVSSIEPVEPLSLIKKPTTSPQQGVTSGGEKDIGSDAEVSVDGSNRSSSSSNSGGNSSNDGNGNIEQNNINIPSLPTADQDIGSGGGDEVIVADQSNDDSTNPTTTTNTTTTSNTTTANTLAKSSSKPTPSALEIAAKYASKVYPITTPYSEGNKYKAMMLSLDGLLDYDETDRHEGTFEASLFAELFFLMLSRDMGKTILNSIVNFRPFVPESSSLSTTISSIPEEPAQENSSTESQSTDKKRKRDDQSPVEEGEDISDESMATKKPSIDGDNENAVDPNMTTTTSNADELNDGDEKTTTTTTDTDQNMKDKDKSTEEQPPSALVNEEEGGITEPSPQESINTNNDNSSNNNNNNTTTSTTNNNADSSISPPNQKNRASNERVADPKIQEAFEFFDQNGSSYLKDEDVETIIHNLGLYLSKSYVQNLLQKVTTEYSSKQSRVYYDKLIYSQNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNN7</accession>
    <name>A0A8J4PNN7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008893</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000595">
        <property type="protein sequence ID" value="KAF2069786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNN7"/>
    <dbReference type="OrthoDB" id="16024at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043721">
        <property type="entry name" value="DUF5662"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18907">
        <property type="entry name" value="DUF5662"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="145" mass="17164" checksum="137C695BF3C134E6" modified="2022-05-25" version="1">MKTTNNTDITNEMREYFYKRTEKHINRVRELMMLMEGYETLKRSDLLERGIAHDQSKYLEPEVTGYIWLSWFHYCKNSNIKFAYPSDTIIEMVNNAVDHHLKSNLHHPESHSNINNMSTLDIVEMVCDWSAISQELNQGSCLNYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNV9</accession>
    <name>A0A8J4PNV9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007985</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000457">
        <property type="protein sequence ID" value="KAF2070704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNV9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035282987" evidence="1">
        <location>
            <begin position="24"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="310" mass="34893" checksum="2C38F60CD6FBB2A3" modified="2022-05-25" version="1">MNIKLILSFLFFVTLANINACTGQTIYALSQKSLNTMSWGFTYKTEVLLDIPIYKIHQVSSVSNEGFLDVITSSSSSEISLIEIDSATGASQTIYSAPLDIDTDQFVDVFYESHFNYVNALYWVAAHDGHLQNTLQLVKIDFRNNQVQNLTTLPLGEANFAGCYDQSLNFYIIVQFQGSLQLNISVYDTEDTTLPINSNIVISPFVSSTDYVILAAEGFLYLYAKIGSSVYVYSIHGYPVKTITFLYSINPNFQNPINNIRVTYNSYVYLDVDSSSESSVYQLELTDMRLVEAFVRTPIVLNSFYDIVVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNW8</accession>
    <name>A0A8J4PNW8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000464">
        <property type="protein sequence ID" value="KAF2070643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNW8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="593"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="721"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="86"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="307"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="382"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="407"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="427"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="476"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="504"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="593"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="631"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="721"/>
            <end position="730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="908" mass="101319" checksum="EC8DAE147BC87077" modified="2022-05-25" version="1">MEVENNNKNINNHNYSNGSTNTTKNKNNSNNNNNNNNNNDMDMTNIDSINPNNNSKNHNVSNINNLLNEEDGHPNDDQTLDYGVTNLLNFNNQNKNMGTNSNNNSNSPNPNNTNSTNNNSNKNNNNSNNSNSAANNAANNAANNAASGYFYIDDSQFQLCRELGYFHNTIIFENLDIESMQFSRISDIVRTSKSTHAKNIVRNSLEKLYNLYKQYDMFNENGNSIIQLEDLLKIKDQDKTKYMPQNSERAGQIIDDLVQTFSLTGENALIKAFRLQYFWHGYSKHCREYMKYNSGNKRGIKRRRKNTTNGNSSSNGTSTSNSNSNSNSNSNSPNQTSSNGNSSQNSNHNSNHNHNSSNSNNNNVRSSSTSTSISNDDDEVETTTTTTTTTTTTTTAKRTGHHHDKSSSSSLSTSFSNNNTVPSPQINGINNNNTNSNSNGNNNSNSNSNSNNNTNNNNNNNNNSASNTPNITPSNHQQQHSQNIANHHLHHQHHQEQKLPSKLPISQSVPSSGTTSPVLDGKPPKPLSTIPTTIPTTIPATIYVPSNVSIDNVTNEFIRIKTKYYPFGWAIAIKNTQGGDFDIDNIQSLNGSNGILKSSDNNINNKQSKDKLEYNDDDYEDDEHINNINTNDDDDDTNIDDDNDDFINDDMESDDDLSTKESNDLIESVSKTLQNYKSNLLSPKKMILGNSNSGGSSNNNMILGNHLNGIKFLNGGVVNNSSNSNNTNNSEVEYDTSDNNNEDVNIKNKFMVDTAIEEKIQLFNTIKLNSNSPILNSLNNNDNSNTNNTNTNNNNDIPLVITGFELKKTKTLTYDGYYIVPIVDSLDPYQKSELIEVQLRTFKYGETIQILLFKTHDEKGEIIEDFDSHPIEIFNYKTTKPSDVYQLELKVTQLEEQITQLKNLKIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP47</accession>
    <name>A0A8J4PP47_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008544</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000536">
        <property type="protein sequence ID" value="KAF2070131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP47"/>
    <dbReference type="OrthoDB" id="21648at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036435">
        <property type="term" value="F:K48-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032435">
        <property type="term" value="P:negative regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903094">
        <property type="term" value="P:negative regulation of protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031397">
        <property type="term" value="P:negative regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46340">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46340:SF1">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="492"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="542"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="630"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="75"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="411"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="492"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="550"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="638"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="688"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="724"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="771"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="800"/>
            <end position="809"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="867" mass="99497" checksum="5B9709844667EE8B" modified="2022-05-25" version="1">MTKGTTTPMTSSNSTTTNNNNNNNNNTIKSGVVGNHDPSFEKVVHYNDQVKTATTNEAGIANNESKVITNGMNHSSSSSLSTSTSTTTVDPSHIPPLPQAPVSITSPLPPSPPLDAFDPLKYAIESKMDETDTESESDEYHSGRYIDFWNKDYTDLTWRCHWLDLQIRDLQYQSLICDQLYSKLLRTKQKMVPDVKSLSNPNEPLQSLRTTSLTKLRKPRKLHRRSVIRISSKQMRNEMESHPLFTFHKGYYKFEDDLKQNINNNLDEKIEANIKKPKVEDRFKLSAYNNYHSDDELDIVSIDTESRKYKKRKDKYKEKEKEKDDKLGKDKEKEKEKESKRPSSRSKSDKEKKKERKEEKKREKEKEKLKEKKKEKEIVEKEDVKRKKRKRHHQQHIESKIHSKRQHEQQAARQQQSKRGASRGNFDLDHVVAPSPGPNFTIPVVHYKEIMTPEWREVDGQVKEFILPSPLLLGSNKSDKLDKLDHHNDIDGNNNINGNSNGIKSIHTTTSTTTTTTTSTTKEPIAKFFTINEEDEMNQLEQQKNNDANNIDDQDTSDEDTDEEYIGRRHAEQEIEEKKRYFRLLMSSSMSAARRKKDLPKEYAEMTVNDLMPSYRFDKKNISLIGKSWNGDLNYIDSSDDSSDDYSDYDDYYGDDDSDDSDSSSNSEESDSDDSSSDLDTDDEHEYNNNSSNHHPSKSSSSRSKNNVHNNHNNNNTHKRSNSTGSTTAVSTKTPPKVSPPMRGKSAAALKSTPTTNSITPAKRKRGAATATLNTQPPTTNRRSGKKQPPNINNIKQQEEQEEQEEQEEIQEKKMDIDTPVGGWWSDYIWQTIEIEDKPGIIKFKRIKTEEIKLDTSNNDNNGNENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPN3</accession>
    <name>A0A8J4PPN3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008172</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000482">
        <property type="protein sequence ID" value="KAF2070506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPN3"/>
    <dbReference type="OrthoDB" id="18857at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF262">
        <property type="entry name" value="FYVE-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="59724" checksum="F36F685AB4138345" modified="2022-05-25" version="1">MSTCIRYLTKALKGHRSLSASAGVRRNTLYTAPTTTTIVGSLTHKSNNVFYNRIDNTKDSRLSFPTTYRLYTTITAETTTKPTTKSFHTLINLQEANDLTVADIETLLNEIPVPAIEFENLIKQHQLEKSNQKEGEKRTYISELEKLRFPNQDYGDDGTGEKTINVQGQATKVQMIDNSGLKATIQSKNGSSYDGQANRDGTPNGFGHLEMKNGDFYIGFFKDGKMSGTGKYEYANGDSFDGEWLDNVRHGHGKSSEMEGKLVYEGMWEHGKRHGPGVFTLPNDAYIKGIWEHDELIFGQEINEDQAEYLGEYKDNQWHGKGKLMVKQSTLYCGEFKNGSPNGQGSWTSANQIVENDNWVDGVANGMGRFVSKGLEVQGNWTNNNINYGVIKRDQHGVYIGEFNKFIPNGSGKEYFVDGSFYKGSYVDGKRSGQGYFQWSDGTSYRGEWVRDYRVGVGKLITKSGDVFQGEFKETLDPQQGLKLCGKGIINKADGSVYSGEWMDGVPEGWGTFTSKDKLTTTQGIWSKGKLINTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPS0</accession>
    <name>A0A8J4PPS0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007036</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000354">
        <property type="protein sequence ID" value="KAF2071643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPS0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052050">
        <property type="entry name" value="SecEffector_AnkRepeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586:SF3">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140860">
        <property type="entry name" value="Pseudo ankyrin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="786"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="877"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="786"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="832"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="882"/>
            <end position="896"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="904" mass="103705" checksum="21CF210043697E4B" modified="2022-05-25" version="1">MTEPMGRGTVLDCSHTTILFEGLKNGESNGESNGESTFNDHPNQVKICINTNDEDEDEDEDGDEDEIEEDSDNHTTDTQSILDRQAKNIHISSEQITAYFFGIFRNQYIRKQIYANLKREGGLKYEQMTIQISIRKRYYGLLDYKIKLGHELGQITYTPYLVTLICKDCQIPSLFRKMYERASSIFLNGHFEAMNKVIVGGNLDNLKMLHNIKELMTDKCASWITKEGPILACKNGHTDIVRFLLSHKQYRDLFSLFALEIAIVNKHTPIVLLVIQEKFDFTLDLNICLKAIESYCQDPATLFTILEASQTHINLLAKPLLISDIVDIDQRESFVLNPKLYPKLSSTAMRQISELLHQITLPASQLAFTADEIKKMASSGNIKVIQRFGLQYDLEDLVRSGNTKLIKNEFLMFIENKNKHLNPKSVSFKVADITAEMGKFELLKFFYRHGLKCSAIGMNHATCRGATKVVKYINKHSGESLACTEDALDGAAANGHFELFEYLFENRVEGCTASSLNSAITKNNEQLLSYLLEETKPEIPKYAKQAAKITRNSKIMKKLRNGFIHNLINLIITRSVLTGCSELYTERFGNTYKPKHTWERIDHLVEYNDLSDFSNERPKFAIQRTNHLNWKTKPDLVMYRKNNDNTRDIKIIDITVIDFATESAIQKQEELVTKKYQHLIKEFESRRNTVQFFPLVFDSAKPISNIQNFMFHLHEFVDIKQIEAIYSDIRVSDFKIPPNPLTNPSNNNINIFHFNNNNNNILNNNFMHNNNIFNNSNILQHNHFHNSNILNHNNNNNNNNNHNHNNNNNNNHNNNNNNNNNNNKNNNFMHNTIVNNNLSNPAPPMNSSGGNINPFVNMNQMASTPTNIPTSISGIFPPENSVPKNNFPTSLNTQNHFHPYPRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPT1</accession>
    <name>A0A8J4PPT1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial import inner membrane translocase subunit Tim21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007006</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000351">
        <property type="protein sequence ID" value="KAF2071673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPT1"/>
    <dbReference type="OrthoDB" id="10547996at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="144"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="337" mass="38781" checksum="371BA023069A5FB0" modified="2022-05-25" version="1">MIGSKFNNILKSVVQINSSKVLSVKPIYNNGSIQFRFFSSSQQNKSSNQYNNKYMIASTKSLLNQYESLYNSINNKNNSRYFSSAPPKDPMEEKLKSMMSEQDKYEQYQKEKKARKEEIEKKRAEMEKEEGEQTKTIGQDGIHKPTTTSTTSTTKPSTKKEEAKEEKKIYDDEHEQSMFAIYSTFTVGFLALLATCVYNIASGFSENDNPFQLPYDKFLIIQEIYHNELLRIFAEGHPDFNQFAGASYSLSEQNMKHQEIGDTFIIVIPIVSKKNYLLGHLCIDIVQEGKVISVGNFFINGINGVIYQLRVPKTKYFTNDPKYSQIPMMPPQEQQDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPW5</accession>
    <name>A0A8J4PPW5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Purinergic receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_008775</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Contractile vacuole membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the P2X receptor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000576">
        <property type="protein sequence ID" value="KAF2069904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPW5"/>
    <dbReference type="OrthoDB" id="494673at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031164">
        <property type="term" value="C:contractile vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035381">
        <property type="term" value="F:ATP-gated ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140417">
        <property type="term" value="F:intracellularly ATP-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071476">
        <property type="term" value="P:cellular hypotonic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050848">
        <property type="term" value="P:regulation of calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.940">
        <property type="entry name" value="atp-gated p2x4 ion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059116">
        <property type="entry name" value="P2X_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10125">
        <property type="entry name" value="P2X PURINOCEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10125:SF31">
        <property type="entry name" value="P2X RECEPTOR E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00864">
        <property type="entry name" value="P2X_receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="314"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2069904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="356" mass="41475" checksum="3E582F928677DC5E" modified="2022-05-25" version="1">MDTQSKVFFLDFFYSYNTKKMVKIRDKRLSFLHLFFVGSIILYVLIGPFLVQQKYLEREIPVGSIRLSLLIPPEREQYLPYCLNETNTMYSGYLNKPCVYWDETLMMYPLSEENSLFITTRVTIKTQEVNNCSLTQRNCTHNTLETSEYYIADVDKSTILIDHTMVSPKLGIQYNANELSGILLDQDDKLIEDIESPNVVGVVGEYDILTLEMILKAAGIDTLDQEGFENSSRSIRDDGMLLFCFITYSNTYTYNTNMFRYTLRFHLIQDSKFKILEPIYLDGVNKRQILNRHGVRIIFIQNGRLGQFDFQTTLLTFISGMGLATFAGWIVDFIAIRLIQEREKYAKMKYQDSDKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPZ5</accession>
    <name>A0A8J4PPZ5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nuclear condensin complex subunit 3 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007705</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000426">
        <property type="protein sequence ID" value="KAF2070972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPZ5"/>
    <dbReference type="OrthoDB" id="27187at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000793">
        <property type="term" value="C:condensed chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027165">
        <property type="entry name" value="CND3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14418">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14418:SF14">
        <property type="entry name" value="NUCLEAR CONDENSIN COMPLEX SUBUNIT 3 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="772"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1106"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="558"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1108"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1137"/>
            <end position="1174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1174" mass="135912" checksum="AE9BA1BABEAB038F" modified="2022-05-25" version="1">MVVAKSTSSKKRVATSVSPSSPTKLTTRKPSTTTTSTTRAILTGSSGKLSSDDVKNDQQQQKQQQLVIQIQTLFLNCQKSIAYHKKSIQVLLDLLVKCSSSTKLIKSYIQIISSIVDRVLAISKRDPPIERVVKFVCNFISTLISSEISTAPTTTETQQQKDSTLLSNENENENNHILLYDQFFNRVMNHLIERCNSKDKSVRFRSTLMIGEVLNSENLREDYEFDEQLWGKLVDTMMDKSKDRIPGIRKQSIYSLSRMQDTSYSDDIVTLRLIELLKFDTSPDVRNTVLQNIIPTKKTLQSILEKTLDINPIVRKQSFLTIGQKIEIKFIPISLRMGLLKNGLQERDKLIQDSCSNMLLNCWLEKTKDILKLLEYFNVEFYEEETELGLMEIFSKGKFGVENCKEFDLFNLSKEQAIYIRVLSQYLKKKEPNNFELLDSFLPSIIKFNDVLLYYLSKSEQQDQENNIDPDSLHFIQSQLILVAQCLDYSDEAGRDLLSKTVKSLILSIGVDDNHLDSLIELLYILHPNPNEYISIIISSLNQLTENIHSQEESDQFQLEQENRLEETNEKLVELEKKKEEIKKSMKSLLKSAASPNIRSIIDKLKDKLTLVRQEYSEQLDLKNEITDSLNIKYIIEQKIWVKTLTILSNFLKHLKISFNHKSISKIINLIKSSCLNHKQTIIVNMAIKVLGMFCLTENNPKDLHDNIEILLELLNKYENQILSPNSSSKSDLTEQIEIFILIIQSLFDILLWYGYKLNSFLYPKKPKQQQQQESLSSKRKLNDDGNNHNSDSDQDEEDDDQVKYSFDYILNRLLVVLKNQQFYSSIQDQGSNLDETIQLTLVEGISKIIYNQIYDPNHRQSSIELYQGLFSYLVFMFFNQNNQGKPIQQVLSSFFNIFVISTRDFNLSSSIIEESFLPSLLLFSKSITEQQSDSKPTTSSTTTSTMVKFFIWLTNSLVSSQIKYRQQQLIQNQSIPQEERKEREREIGVELVSFENQVYFNFVYKLSLETLSDTSIGKNYCKLFGLFQLDSSVPSKQIKQLLFLASKMLSEIRAKTFYNQLFKYHQYLVSLDLDKDSHLSDADQDHLNELVDQFKQISNSKSTLTKNIKKRKLNNTSRSTKKKMVTKKKSKKYSSEEEEEEEEDTEDSSSEEEEKGSENDDDEEMMEFEVDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ77</accession>
    <name>A0A8J4PQ77_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007867</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000443">
        <property type="protein sequence ID" value="KAF2070820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ77"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="102"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="146"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="292" mass="34018" checksum="7EF5690D40FDCF43" modified="2022-05-25" version="1">MNLNRYNEEETVDGNFNSFLNSPSMRFVLRTTTNTNDHYPSTTSFLTDTNQFNNNTGIGKSKDNIIRNNNSSGGGSSNNSSKDDQDDFIRYLDEVFKTIQSSPSYNNYKNYNSNYNSNSQPSTQQQQQQQQKANDESLFNGKHQKQQQQQESPQLTSQQLYNQVSTIQHQPHTPPQSSHRHPNNYTDHSNQKIQHKPSQQQHQQHQQNQQQHQHQNQNQQSTTTNSKEDIKKNSLLNLNFVIQEIMHELQDLRKQLKLTSNPELIQLLSRSINSKEQQIIKLEHNKRLLLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR64</accession>
    <name>A0A8J4PR64_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">isoleucine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Isoleucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_007922</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000449">
        <property type="protein sequence ID" value="KAF2070759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR64"/>
    <dbReference type="OrthoDB" id="10264412at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07960">
        <property type="entry name" value="Anticodon_Ia_Ile_BEm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000152">
        <property type="entry name" value="Isoleucine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.830">
        <property type="entry name" value="Ile-tRNA synthetase CP2 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02002">
        <property type="entry name" value="Ile_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033708">
        <property type="entry name" value="Anticodon_Ile_BEm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023585">
        <property type="entry name" value="Ile-tRNA-ligase_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050081">
        <property type="entry name" value="Ile-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42765:SF1">
        <property type="entry name" value="ISOLEUCINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42765">
        <property type="entry name" value="SOLEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="10">
        <location>
            <begin position="83"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="11">
        <location>
            <begin position="740"/>
            <end position="895"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2070759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1013" mass="115012" checksum="BA897E032F8B3F2F" modified="2022-05-25" version="1">MNSVLLNLRKSLNNPSFLTSNSNHGLVSLKNHYCKSSSSATDTNHSYANTLNLPKTSFSMKANAVTREPTLLYDPYKLYLWQKDNNKGEDWILHDGPPYANGDLHMGHALNKILKDIVNRYKMLRGHRVNFIPGWDCHGLPIEQQAFKKIKKGNMSAVEIRKIATDFAMNEIAKQKKEFRKLGVMGDWEDPYMTLEPRYEVGQIQTFFDMYSKGYIYRGVKPVHWSPSSRTALADAELEYNEAHVSKSIFVKFKTTSLSQGLADALKGTSVSVENLNAIIWTTTPWTIPANLAISVSPTINYVVCKGNNNNEYYIVSQERLDSLRKSFSMPLEVVAEIQGKELAGTVTAHPQFNRRSPIILGDHVIEGSGTGLVHTAPGHGVEDFQVCKAHPDIPVISPVDDAGLFTIEAGEKFVGLDVLGDGNEAVITDLASIDALVFQEDYVHKYPYDWRTKKPIIIRTTNQWFVDLQNVQQPALESISRVNFVPPSGTNRLSSMVGKRNDWCISRQRVWGVPIPVFYRKDNDEPLVTDQTIQHVKEVFAQHGSDAWFSMDVKQLLPKEMESVADQYIKGNDTMDVWFDSGTSWRGVLVQRGIIPEDGAADLYLEGSDQHRGWFQSSLLTSQCVRDREPYKNVVTHGFLLDEKGIKMSKSIGNTIVPATVISGGSNKAQYPGYGVDLLRLWVASSEYSRDVSIGPTILTKILDSIKKLRNSQRFMLASNFDFNPALHTVPYEQLSDIDKYALHKLHLLQDSINKSYDAFSFQKVYNELINFSNEMSAFYFDIIKQRLYVENPNSLQRRSSQTVLFEILETVNKSLAPITIHTSEDVYRHQFHFNNPSDSIFMKGWFNIKQEHNNVSVYKKFNNIITVRDSINRILQSMRDKGIIGRSDEAKISISITNGPEPLYDILSSMSNQLNDIFCVSGVEVTLDSNNYKEPEQEEKDKFENSPITIENDQFELDQYLSLNLTKYMGKVKITGKRSDKHKCPRCWRHQSETVDHLCNSCDTIIQSLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRY6</accession>
    <name>A0A8J4PRY6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005323</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000209">
        <property type="protein sequence ID" value="KAF2073353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRY6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="520" mass="59982" checksum="6DB38493A8DBBC6A" modified="2022-05-25" version="1" fragment="single">IKDLDHFHIYNSHQNKHLITHIIVSSHFYVNHYNIVQKQPQHQQPILFGLSITTPEDPLILATELPLSIKYFKYTTVSQRLTKEYLPQTITSLYIDISVSIDQIPKSVKTLTIDSSYQSKRDEKRLENMKFHHLSQLLPPSLTKFQFNDFSHKAKIDFNLNQMPPNLTYLAIFGDFTFTGNGNGSLKYLKLNTKVSDRPYLIPTSVTQLKMTTPSINSTLLPPNLNHLEYNFIGLHGETATAPNKFPEKLTTLSLKNCSFTNISKNNWPSSVLDYRNWSEQSEKDVPYLPPSLTKARLSIHNDMKEFPILPSSLIELKMVSTRRFETFAGPSPLHSYFPNTIVKLALEYSDELFIGLIPTSVTDLKLHYSKPIPKGAIPNSVTKLHLFNSSSDLAKPIVIPNSVTYLNFEFERQLYPELEIPDSVKTLKIKYMEFLKITPFLNYFKTTFKNITRFSTRSSIYKKIKLIVEKLRDKKLFPNLKQINVVGGNYHFTDQNMVYKEVDYKSNSVGSSNFKNKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS51</accession>
    <name>A0A8J4PS51_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_005197</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CCC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000201">
        <property type="protein sequence ID" value="KAF2073488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS51"/>
    <dbReference type="OrthoDB" id="73465at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008217">
        <property type="entry name" value="Ccc1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31851">
        <property type="entry name" value="FE(2+)/MN(2+) TRANSPORTER PCL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01988">
        <property type="entry name" value="VIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2073488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="281" mass="30687" checksum="AF9D3201A9FD8CE6" modified="2022-05-25" version="1">MPPQQKSASINSNEKSSIINESIPLLAKSSPSSKPSNKFELSKYLKSIIYGGMDGLVSIFVSIAVVASGDASIAVLLVIVLSKLVAGAISMGMGDYLGTQADVDYAKGERNREAWEVEYYLEGEKREMVDIYISRGVPSDIAQEVVDILSKNPKGFVDVMMVEELGIMPDAEQESPWMNGMVNFISFLIFGIVPLLPYLVYLLVADLDHLSNNQNLPTFLAVIGLTIVTLFGMGIYKATSTNTKWWKNGFSTVFFGCIGAGVGYLTIFIIKKINPSIDISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSI6</accession>
    <name>A0A8J4PSI6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_010025</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000889">
        <property type="protein sequence ID" value="KAF2068651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSI6"/>
    <dbReference type="OrthoDB" id="442460at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.130">
        <property type="entry name" value="Ubiquitin-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47764:SF2">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEASE FAMILY PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47764">
        <property type="entry name" value="UBIQUITIN-LIKE-SPECIFIC PROTEASE 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="5">
        <location>
            <begin position="406"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="59"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="132"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="342"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="695"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="87"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="133"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="350"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="727"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="841"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="882"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="920"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="943"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1014"/>
            <end position="1024"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2068651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1024" mass="116014" checksum="938187E36D517B43" modified="2022-05-25" version="1">MTSVESADEEDLSYITEVGKKEVILTPGKPSTQHIRPYSSPFSLPTERVNHVNSIISKVTQTPNEISRNSLSHSRTPSPNKTPVPTPVTSIITTTTTTKRPSYQQSSLSNSSPINLDDDIEEENDVKRITPIKLQQQTQPSSFKQNPSLIQKTTTSTIPQTRGSTRNDINSNNISYTALLTKLNHAENDDAFVVELNAKEIQVPKTPPHVSFERCFFFKESSKPRCLIRCLHNLSRMGFVYDIYPIDSILINKEYKEVKIQMNRYQHILLNSNQEWKPQVVGENDPHYFLFKDVEITEILIEKLNQLCQCKVIEPKSQSNGDISVSEDFKKQYNDLKILDQKKRPPLAVNNNNNSKSASNINGGSFGDFNSSNNNSISKPIGVNGYGGSNGSTKNSAYTSSFSKPSTIHELDLPSPIPQKKTKYKCSDELMIIYPVSNVDKDNKLVAQVRIIRGDLQRLNQEEFLNDKEAYREIKKWTGNEDIFQKDFIFVPINQSQHWSLMILCFPGESLDTDPEKRIDYKRRPCMLYLDSLYKKPGVFPEKLRSYLTLEWMNKKYGNGTEEKRKYTKDNYQLLIPHVPTQKNFSDCGVFLLHYIELFCKNPEKNFKNPLENPAWFKNSEIIQKRDVIKSLIFKLRKEQDPNAETEEEENKFIIRLTQPKDTTTYADTSSLEIVSPIEDFESPKTTTTKTFKSILEKEKEEDPIESDGSAKDKEQPKSQIFSAMDKTSKIPKKIDTMKTGDEKNDFNDGKYGSKWNSNWSEDDDDGQKEKLKPNTSDDDQMRKVGYNPLASSSIHFRPTRPVVVPEQSLNGYDPSPPTSTTSTTNSNDKKTTHTQKVSTSRREPLKKQESSSESSSEESESSESSSESSSEESSDSDTKSKSKKKKVTITKKRQLSSESDSDTDTKAKVKPKPKPKVTTTKERRKTESDSEPSSPESDAKSKKVLANKITKKRPNPSSSSEESDEKAKKKSKPTLAVSKAKPHAKLKPQPPISPKLISISDAEDDSEISKNDDLDDSIDQAFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSJ6</accession>
    <name>A0A8J4PSJ6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_010011</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000884">
        <property type="protein sequence ID" value="KAF2068666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSJ6"/>
    <dbReference type="OrthoDB" id="364892at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01681">
        <property type="entry name" value="aeEF2_snRNP_like_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04096">
        <property type="entry name" value="eEF2_snRNP_like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16261">
        <property type="entry name" value="EF2_snRNP_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1430.10:FF:000002">
        <property type="entry name" value="Elongation factor like GTPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000010">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000006">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000003">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000002">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000058">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1430.10">
        <property type="entry name" value="Yeast translation eEF2 (G' domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF10">
        <property type="entry name" value="EUKARYOTIC TRANSLATION ELONGATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="7">
        <location>
            <begin position="17"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2068666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="837" mass="92612" checksum="88A51E6A09AB5951" modified="2022-05-25" version="1">MVKLTIDQVRALMDHRENIRNISLIAQANHGKTTLSDSLILRGCILANNVAYYQRYIDMPQEERGSDVKSSDVSLYYEIQDTQDLPNDTTNSAFLINLIDTPGHVDFSSEVTAALRVTDGALVVVDCVEGVCIQTETLLRQSVAERIKPVLFVNKLDRFLLELELNTEDTYGSFRRTIESVNAVIGTLQDELLGDITVAPEKGTVAFGSGLHGWGFTLDKFAKLYAAKFAVPKERLMARLWGDNYYDVEAKKWTTDNVSVSGKHLQRGFCQFVLEPIYQISQAVLSENMEKVNKMIEALKINMDRDDMLELKGKALLKAIMSSFLPAGDAILEMVVLHLPSPVVAQKYRVANLYEGPMDDECAVAIANCDPNGPLMMYVSKMVPTIDKGRFYAFGRVFSGVIRAGQKVRIMGANYVPGKNEDLFLKSIKSTVLLIERRPEQIEDCPCGNIVYLVGVDQFLVKSGTITTSEVAHNIRAIKFSVSPVVRVAVQPKNPSDLPKLVEGLKRLAKSDPCVLCYSEETGDLIVAGAGELHLEICLNELTNDLAGVELKTSDPVVSFRESVSAESSIVCLSKSPNRHNRIFLKAQPMAMELQNEIEDGSISATDEVRSRANYLTDNYGWDNNDGKNIWSFGPEGVGANILVNATKGVQFLNEIKDSFVAGFQWATKEGVLCDENMRGIRFNLIDVILFSDAIHRGAGQLIPPTRRVLYAAQLTASPILLEPVYLVEITAPENALNGINDVLNRRRGHVIAEERRIGTPLFSVKAHLPVLESFGFTTDLRSHTAGQAFSQCVLDHWSSLGVVGVDKKASEVCTSVRKRKGLKEEVPPLDNFLDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSV0</accession>
    <name>A0A8J4PSV0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Profilin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_005539</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the profilin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000223">
        <property type="protein sequence ID" value="KAF2073150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSV0"/>
    <dbReference type="OrthoDB" id="421374at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003785">
        <property type="term" value="F:actin monomer binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045010">
        <property type="term" value="P:actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030837">
        <property type="term" value="P:negative regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030838">
        <property type="term" value="P:positive regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00148">
        <property type="entry name" value="PROF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.30:FF:000001">
        <property type="entry name" value="Profilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048278">
        <property type="entry name" value="PFN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005455">
        <property type="entry name" value="PFN_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036140">
        <property type="entry name" value="PFN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027310">
        <property type="entry name" value="Profilin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11604">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11604:SF0">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00235">
        <property type="entry name" value="Profilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00392">
        <property type="entry name" value="PROFILIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01640">
        <property type="entry name" value="PROFILINPLNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00392">
        <property type="entry name" value="PROF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55770">
        <property type="entry name" value="Profilin (actin-binding protein)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00414">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2073150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="126" mass="13369" checksum="E075DD47ED3DDEED" modified="2022-05-25" version="1">MSWQQYVDEQLIGTQNVEEACILGLDGSTWASSKGMGLKAGEGQGLAALYKNPANAFSQGITVGGVKYMGIKADERSIYGKKGATGIAAAKTNQCIILGRYNEKQQPGNAALVCEKLADYLIDNGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT27</accession>
    <name>A0A8J4PT27_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RasGTPase-activating protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_004627</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000167">
        <property type="protein sequence ID" value="KAF2074058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT27"/>
    <dbReference type="OrthoDB" id="17673at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF149">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GAP" evidence="3">
        <location>
            <begin position="167"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2074058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="469" mass="53444" checksum="351577EBCAE22905" modified="2022-05-25" version="1">MTELPPIGRVYLNVFEARNLIPMDIGNSSDPYAYVTIKSADLKQIMSSTLRTKIVYKTLSPIWNDEFVFDLTHFVAGARETFYIELWDEDKISKDDFEGAACVDILDYINKGITDVWIPLTGKNNTPSKKNRGDVHIYFKYVPFNSLMDTLSSDEHSIVRHLSRQLVTDEFTKALMYYIGNYGFLVTSIKDIIKQEIETTSDPNTLFRTDSLATKLTILTFKTIGSNYLRETLQHLILNIQQNNIYLEVDPSKVDEAQCAENIGKLTDMVEKFLDAIYKSIDIVPFEIRTLCKYIYQQTGLQFPSDNTNIKAVGGIMFLRFIVPTIFSPETLSITPEAPNSNVRRTLTLISKILQNVSNQVLFGSKEDYLMSFNTTIQQHIERLSAFLIEIVTINDSISDSMAALQQTKSCLKQSLKCDPTQLAKYTDTIITAIHDKKQNLIDKPTLKKELVDAIVYQHSLIAKRDARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTE1</accession>
    <name>A0A8J4PTE1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ras guanine nucleotide exchange factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_005330</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000209">
        <property type="protein sequence ID" value="KAF2073360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTE1"/>
    <dbReference type="OrthoDB" id="28357at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF368">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="267"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="381"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="541"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="8">
        <location>
            <begin position="722"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="7">
        <location>
            <begin position="895"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="58"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="103"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="214"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="601"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="633"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="69"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="103"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="114"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="135"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="214"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2073360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1138" mass="130128" checksum="4D7974509CAE66B9" modified="2022-05-25" version="1">MERVKHLKVPPTNYYGNRSRSASIDMIVMDEISKNGNWTTNNTTNNPPLVVVGDLDVNNNNNNNNNNNISPNLTSQSSGSTSAQNTLSQAGVFHINLSQLNNNNSANNSANNSPRYFSSLSSLQQPTHPSPPVSPRTQMTAPNLSTFIAQHTPPPSPKPSDDLRHYRHSNHLSPPPFSPNPLHHSGIYRNLSFNSSINSMSSIDISSKLLLQQAQQQSQLSNNNNNNNNNDTSDSNNNGNELTQSKIINARRKRKKQNKTIKRFRRVLFKIINSHFWIGLMVLAIIYILFIDDIIGISGSPSSNSIDVTIIVIKVIVLFFFTVDIISNAICYGRQYFPGTIIFWFDVISVLSIIPDIVVYSNNSFLSPNLHFLTIGKSARIIRICGCLVNVSLISTIYNRFLRTKHHVPLPSDGLEVEASKLGEKLLNLTTNKIVLLVLFVFFATQLFVYQSNVPQVYVESTLSTFEYMVSTFGYNSTLFENFTNSYIGASHNRVLRLVIGSHYIIDQSIDNIQNNAVLKYSFSKSSLFINNTEYIKYTSILHLCLTIFVIIILLIVNLLIVNDSHWLVINPLENVLTIVKFLSKQNSAMSKASAVRSRVESIGNKSEDTTSSSGIEDAEEPENEADFLLGMLGDIDDSLQAAKEKVEEESNQNTILKKDIEDLFVEKYILQIHLRSLIRKITFSDPIGMYLKTKQLRLLATGDDNESDDIRYKCDQENNVDKSLVLAGTIDKLVENLSIPEVQDLKFANVFLLTYRKFLSPTELLERLIIRFCVTPTMELPEKLLVSKESVEEWRKAKQEQIRVSVFNTIKLWISKYNFDFYSNPELYELFHNLVHKIMPFCRMDKYATHLDTFFKKKMGSFKPEREYIAPVPLQAEEIAQMMVLDDRLLYNFEINDIAIQMTLIEFDLFKVIKPQEFLDLAWTKKNLKAKLSPNILRFIDHFNNVSFWLQTLIVRSGKVKERVSILKKVISLGEHFIQLNNFYGAMEVLSSIESSSITRLHKTWEQLPLQSTQSLAALKKLLSPVGSFKDYREKLRQTTSACIPYIGIYLSDLTFIHEGNPDYRDDLINFSKQREIASTIISIQQFQNTYYYYETNERLRSQLLDIKGIDNDTIWKMSLTIERREERGEKKIKKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTE6</accession>
    <name>A0A8J4PTE6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ER-bound oxygenase mpaB/mpaB'/Rubber oxygenase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004392</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000154">
        <property type="protein sequence ID" value="KAF2074298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTE6"/>
    <dbReference type="OrthoDB" id="545169at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046366">
        <property type="entry name" value="MPAB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36124">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36124:SF1">
        <property type="entry name" value="ER-BOUND OXYGENASE MPAB_MPAB'_RUBBER OXYGENASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="330" mass="38276" checksum="1D244635D2BD94BA" modified="2022-05-25" version="1">MIEISTILSYLPGFLISVFIVYSTYNYYQRKVAQKKWEKRFEELDIKNNDHVDEIIKQIVGFDFPLEMFLSLNFAFYRTFCSPTISNVYKKTGIIAGDCEKRANDTDLLMHTWMDYGVDSEEGKASISHLNKIHGLHAKVTRNVDFLFVLCCLAADACDFCESYGSRKIQYREQLAIWEFYKRVGARMLLKDIPNTLEDCRKFIECYTEDNRQSRVNPDGGMLTEAFTKLVIHWYYALPEFVCRMAVSVLIYQMSETFHRKLDLAKPKPWEFALINGVLSIRKNVLKILPPRTVPYKVSDEVMNGFYGCPVSKETLSKVGPIDMLAKILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTV5</accession>
    <name>A0A8J4PTV5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ABC3 transporter permease C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_006230</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000278">
        <property type="protein sequence ID" value="KAF2072454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="312032at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32522">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32522:SF5">
        <property type="entry name" value="ABC3 TRANSPORTER PERMEASE PROTEIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="640"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="683"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="732"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="792"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="824"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="886"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1235"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1283"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1328"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="domain" description="ABC3 transporter permease C-terminal" evidence="8">
        <location>
            <begin position="644"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="domain" description="ABC3 transporter permease C-terminal" evidence="8">
        <location>
            <begin position="1239"/>
            <end position="1351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="24"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="61"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2072454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1365" mass="153144" checksum="3D45025346F6BD56" modified="2022-05-25" version="1">MSKNYDDHKSHLLGDNHDGFGEFDSSFNNTFQSNSNSPQFNNGSSKYQKLRDEDMDDNDDSSPSTYSNPHTDSPFLKSNGLGGKTSSLSSFETFSLGHGHKIDREKDDGSSSFNMSTVKGFFQYLYVSLRYTLYGDLRVALKYVHNNSKRNKKSLLLGLFTIFLVVLFISFLQNLIQASPIVFLKLSEDQTGELDLVITGDPNNYGSANTSLLLATPVYETSSEEAQYNKVDTREKLAEAMDENDFNGVSTDQQISRATSLLDVVKDTVASVNNQLNFIGDLSGYFVNSTFLNQTLYGASTVHGVAPRWLSIGNIKNANTDISASLILMVMNTNLEKSLNLGRGWTHPALVGNQIHITKSFLTRIGVEPNKGESVHLHMSFVDVLERLGTQALPTVKDITNVIEQYTQQPIPDSFNITALVDIYQSIYPSKNMTQVLTDMFDNGINITKIDATTYSYIIFPKIIISNYYSFFENDLTIDKELVVVDAIKSSGGKFPNSIGNVGVLESSFVEQLIVEKLDNISQQLNSSVFQNIVQLAEFVESIYPNNNYSMIIDQVINLQDRITNFTRQFKIDYYSMSSVVMLQNRVKIYTASDQEMMNGFMAFTNQIASRLGYSYPVTFTTPLSTTVQMFMYTKLSLNQIFNCVATVLLVLGALMIYSLLLSDVEGKTFEYGMLRAQGMRHYALILLLLSQALYFSIPGIIFGLFFGWCVYSVVAYFVYCKFVLLPIDLTFYSTSIISGLFMGLLMPIVANIAPIQRALSRTLRDALDVYHQVKNETIVKIMKLEEIGLDVLQTLLAVMAVGVGFTVYYLIPLSFTFRNYGLFFGILTGILMGMLFGMSMLAQAIQPFVEKAVIFCLILGKDRKSLYNLVRKNLFSHSSRNSKTATMLTISLTFVIFTGCVFRLQGHNIQELVRLGIGSDVAVVATSTFNPIPEDKIRAFLDNDIANNTQSIVTSYSTVTFPLDKIMNIRSTHLTTLASYPDVPVRVYGVEQNFLSSVFLDFYDYTELSKSLNFPSIKGTSTPDVISSLYVNQHKQTIPEDVGGVIVPPPSVISNGSVLYEPDNPIWWWVRNHLNNTYVYKNYTDMVISEAFRLTSGADTNTPFSLVIKYKQFEGALNVLNLLSKPQSMVGMFPSFFFSTYSQTSWNSPVLVNIDEFYNIMNLVFSLTSDPAVQLPAVPPKSKILVKLKASATQSQRDNVINGIRNFIKTDNIQVVDTQFLLNNTETAITILNLFFYVVSSASIILCFFMLWVSFSANIHENSWEFGVLRSIGLTSFQVTRVYIYEALVLIFSSMILGLCIGLGIALTLTLQFDLFTELPFSFEFPYIMFIGVLISSIFIAIFVSYQSSKEYRERMIASVLKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU09</accession>
    <name>A0A8J4PU09_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">LMBR1-like conserved region-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_005280</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000206">
        <property type="protein sequence ID" value="KAF2073401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU09"/>
    <dbReference type="OrthoDB" id="73273at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006876">
        <property type="entry name" value="LMBR1-like_membr_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31652">
        <property type="entry name" value="LIMR FAMILY PROTEIN DDB_G0283707-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31652:SF0">
        <property type="entry name" value="LIMR FAMILY PROTEIN DDB_G0283707-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04791">
        <property type="entry name" value="LMBR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="199"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="312"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="411"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="458"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2073401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="491" mass="55254" checksum="13ABAD84587CED40" modified="2022-05-25" version="1">MVNVFMIIVSVVVCVLVILASLYLIVYFQHPDDKNVAYFPKVIVILGLVLSAISILMLPLDVANNGGEGGFPMDMMWIVVYIIVGVFAIVICPFAMFFYESEEGDPDAKSGQFAGAVKGTMAILFVFAVLTIIFYIFFGVAEIPTIAVSAKFIQTAGVPAADATNTTNAKYIDIVSTTTPQTTEENRLRKETLQFRVSIALFIITMVSTLGWLLFVVFGGIGLIALPFDMIADFRNRPQRIPYDKYLERKQKIGERATNLYDIGKEIQGRSPGGVMSKKDRRNYNRFRQAVFLLEEDYERLKISYKRQGGKVILYYAQFFGGFICLGISVGWLLHIIIFMITQPEPFNPFLNNLVVALDEAWGFLGTIVYGLLSFYLLLCCIKGNFKFGLRLFFLFPIHPMRVGETMMNAFLFNVGLILICSVSVTQFCSMAFSNYTSLTAINALFDTSVRNLKVLKYFWLVYIFAIFIMAVLTGIFLLVKPKDKPAKIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUY7</accession>
    <name>A0A8J4PUY7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RGS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_005020</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000190">
        <property type="protein sequence ID" value="KAF2073670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUY7"/>
    <dbReference type="OrthoDB" id="194358at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07440">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050745">
        <property type="entry name" value="Multifunctional_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24189:SF50">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24189">
        <property type="entry name" value="MYOTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="173"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="208"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="RGS" evidence="5">
        <location>
            <begin position="277"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="399"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2073670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="502" mass="56440" checksum="1C57CB7DCCEB6B6E" modified="2022-05-25" version="1">MNINPLSFFSGKDKEYTEEELKQDNKLLHKSVKDGNTKQVKKILGGKKGKQLLTMVDELDQTCLMNAARNNNIEMFELVLSHYRICKVDINLPDKNGYTCLHHFFSMAADIDIKMVIILLEADDIQVNVTNRDSNTPFHYFCQKFRAPNNAILKEVFEILLKKNVNINAINKNGESPLHKAIFNTTVAQGMITLLLENKANVNYINKNGESPLHYCVRLGRADLVMILLQYGADRTIIAKDNKPLYQIAIEENFTKLADLLRDGGDLSQINDSSSTELLSLLQNPTLREDFRVFLRDELICEENINFWTDVEAYKYLKVDANRLLKEFHRISEKYLAESSPSEINVPFSIKFNIANFKKTLPTVDSNPSSPILSGSNNSSNSNSNNNLNNCLDSPCINSHNNNDNSSNSNNNNSNNNNNNNNNNNSSFSIAVSSSPSFTNNNGSESPTISGISPSTSSCSLSMSAVDYDKLQKIFNVAQHHIFNLMASDSLSKYKKKSFLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PV37</accession>
    <name>A0A8J4PV37_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_004567</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">JanA and janB regulate somatic sex differentiation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the janus family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000164">
        <property type="protein sequence ID" value="KAF2074125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PV37"/>
    <dbReference type="OrthoDB" id="10249612at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101006">
        <property type="term" value="F:protein histidine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007548">
        <property type="term" value="P:sex differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.20.20">
        <property type="entry name" value="Janus/Ocnus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007702">
        <property type="entry name" value="Janus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038596">
        <property type="entry name" value="Janus_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12258:SF5">
        <property type="entry name" value="BCDNA.GH02250-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12258">
        <property type="entry name" value="JANUS-A/JANUS-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05005">
        <property type="entry name" value="Ocnus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143724">
        <property type="entry name" value="PHP14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0726">Sexual differentiation</keyword>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR607702-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR607702-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2074125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="160" mass="18389" checksum="09B31271477F43CB" modified="2022-05-25" version="1">MSNQDLFTNAVVYKSTTTPNRTFQCIIDHQDDEKYVILRLTPYDTTTNSEAIEDRWVLASTETEAYHSDIKNWYCSNTADAHKNIWRSPVCMGGGIIKVDHENKTVKTWGTSGGYGDPPLEIVEQILKTVYEPQGYQLDIKITDEVRELIKPKHRGYWAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PV97</accession>
    <name>A0A8J4PV97_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RGS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_004377</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000153">
        <property type="protein sequence ID" value="KAF2074317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PV97"/>
    <dbReference type="OrthoDB" id="21584at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07440">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845:SF192">
        <property type="entry name" value="DOUBLE HIT, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845">
        <property type="entry name" value="REGULATOR OF G PROTEIN SIGNALING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01301">
        <property type="entry name" value="RGSPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RGS" evidence="4">
        <location>
            <begin position="870"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="351"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="459"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="632"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="709"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="987"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="352"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="465"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="486"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="502"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="709"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="747"/>
            <end position="757"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2074317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1009" mass="112866" checksum="CB1FF4EA82575856" modified="2022-05-25" version="1">MDNNLIHQSTTNIHGESLDDFQSLKDKIVGNTASGGGGNCTRERIEQLELRLDYECFGAYNFLNLNNSNPINNHKLSPTDSSFLSPNSCNTTTVSCHASNMAMSNSSPPNSNRSLSYISLASSNTINMHSSISTSGKYSFYQTPMSSSSSFSDISSLSNCSISTEMSMGTLSNGNQGIIIDFSNRDFTKIPNRLPCEKEKIECFNCSRNKISIIPDDFSLNFYCLITLNLDYNKFKKIPSAIFKMESLSFLDISNNKIESIPKEIEQMSSIKELYLGNNLIESIPFQISELTLFIFDIDNNPLTCPPMEIVEQGFESIVDYLVEQNGRNSNQPSNTLTSSTSLYEKKKIKNNSKNNSSNQNNLSPPTQNVNTQQNNTKKIDSKTYSKADCKLFTADGHSMVIQSSPTAITNTYYRPKKMKQRPPSPKNSKDIKNLIEIQQSLLLLPEVKINSYNSLKSSHKKMDSSFSLSKLPSFSKDDEKDIDQTDSNLSVSPTSPIFEKLKHKLSKKDKKNNHNNNNSSKKKEDKETTPISALKPISSEPTMFDDDTNVKYLDNHHSLSKQDSQEMSIKEILNSFNKFNSNSIMSNTDYSIVQQHNTYKKGLNSSDENESYLNHTTTDSFDKSDYFADDSSIDSTKSSPTEDSKLEETIGFEDYENNECSSMKNQSLASMLSINTTKVISNISQSQSTSQPSTPTSVVSLTNLFTAPSSTSEPLSSSCTTPSPTLNQSSSIPNQLSSSSSSISSVGTMPRSQQKQPVFQKMATVFHFGGKSNNFKNQYNTISSRNTAPLTTSSPIFSHQTFLPCPSPNQSPSATIKAKGLRNSTALEKHDIDTAEVSIFPFGVNRERRGTIASELDLKNHCTTIRFNNLLEIIKDNKAIQVFRTFLIGEYSVENIDFWSCVENYKKTENKTNDMSNSIYEEFITDNSINQVNLPNNVVKRIEENLKQQTNIETVFDEAQSHIFKLMESDSYERFKKSSLYFQLNQNTNTSSPPHKDGSSSPNHYLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVC7</accession>
    <name>A0A8J4PVC7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Superoxide dismutase [Cu-Zn]</fullName>
            <ecNumber evidence="7">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_004798</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="7">Binds 1 copper ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the Cu-Zn superoxide dismutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="7"/>
    <dbReference type="EMBL" id="AJWJ01000176">
        <property type="protein sequence ID" value="KAF2073910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVC7"/>
    <dbReference type="OrthoDB" id="14286at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00305">
        <property type="entry name" value="Cu-Zn_Superoxide_Dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.200:FF:000001">
        <property type="entry name" value="Superoxide dismutase [Cu-Zn]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024134">
        <property type="entry name" value="SOD_Cu/Zn_/chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018152">
        <property type="entry name" value="SOD_Cu/Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001424">
        <property type="entry name" value="SOD_Cu_Zn_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10003">
        <property type="entry name" value="SUPEROXIDE DISMUTASE CU-ZN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00080">
        <property type="entry name" value="Sod_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00068">
        <property type="entry name" value="CUZNDISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00332">
        <property type="entry name" value="SOD_CU_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6 7" id="KW-0186">Copper</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Superoxide dismutase copper/zinc binding" evidence="8">
        <location>
            <begin position="13"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2073910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="155" mass="16151" checksum="DD731161F5807D56" modified="2022-05-25" version="1">MTKAVCVIKGDTVQGTVKFTQENKDAPVTVDYEITGLAAGLHGFHVHAFGDTQQGCISAGPHFNPHGKEHGAPTDENRHVGDLGNIESQGPEGPTKGTITDKVISLFGEHSIVGRTMIVHADKDDLGQGGFEDSKTTGHAGARLGCGVIGLAQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVG6</accession>
    <name>A0A8J4PVG6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MD-2-related lipid-recognition domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_005074</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the intermembrane transfer of phosphatidylglycerol and phosphatidylinositol.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NPC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000193">
        <property type="protein sequence ID" value="KAF2073617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVG6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015918">
        <property type="term" value="P:sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003172">
        <property type="entry name" value="ML_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039670">
        <property type="entry name" value="NPC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11306">
        <property type="entry name" value="NIEMANN PICK TYPE C2 PROTEIN NPC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11306:SF0">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROL_PHOSPHATIDYLINOSITOL TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02221">
        <property type="entry name" value="E1_DerP2_DerF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00737">
        <property type="entry name" value="ML"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035327883" description="MD-2-related lipid-recognition domain-containing protein" evidence="7">
        <location>
            <begin position="21"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="MD-2-related lipid-recognition" evidence="8">
        <location>
            <begin position="24"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2073617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="141" mass="15421" checksum="AB62A2FC5BB3DD94" modified="2022-05-25" version="1">MKAIFVVFLCFAVFAPAVFSFNDYSDCGSQAFSISSIGLSPEKVVREKQFNITVFGALKKDADKVQVGLEIKFNDTVLIDKKSDLCKLVKTCPIKASNISLPISAVLPKNSPAGQYQVRISATNDKNEKIICALVKINLDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVR3</accession>
    <name>A0A8J4PVR3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ADP/ATP translocase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">ADP,ATP carrier protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_004590</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell. Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane.</text>
    </comment>
    <comment type="function">
        <text evidence="16">Catalyzes the exchange of ADP and ATP across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ADP(in) + ATP(out) = ADP(out) + ATP(in)</text>
            <dbReference type="Rhea" id="RHEA:34999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:35000"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 16">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000165">
        <property type="protein sequence ID" value="KAF2074105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVR3"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005471">
        <property type="term" value="F:ATP:ADP antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140021">
        <property type="term" value="P:mitochondrial ADP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990544">
        <property type="term" value="P:mitochondrial ATP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000002">
        <property type="entry name" value="Putative ADP/ATP translocase 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002113">
        <property type="entry name" value="ADT_euk_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635">
        <property type="entry name" value="ADP,ATP CARRIER PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635:SF14">
        <property type="entry name" value="ADP_ATP TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00927">
        <property type="entry name" value="ADPTRNSLCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0050">Antiport</keyword>
    <keyword evidence="11 14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="9"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="113"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="214"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU368008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2074105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="307" mass="33278" checksum="F795F678DE9674F5" modified="2022-05-25" version="1">MSQKKNDVSSFIKDSLIGGTAGGISKTIVAPIERVKILLQVQSASTQIAADKQYKGIVDCFVRVSKEQGVVSLWRGNLANIIRYFPTQALNFAFKDKYKKFFVRHSPKESPMKFFVGNLLSGGAAGATSLLFVYPLDFARTRLAADIGTGANKQFTGLGNCISTIYKRDGLIGLYRGFGVSVGGIFVYRAAFFGGYDTAKGLLLKDPKKASFWASWAIAQVVTTAAGVISYPFDTVRRRMMMQAGRADILYSSTWDCWVKIAKNEGPAAFFKGALSNAIRGSGGALVLVIYDEIQKMLGFEGGVGSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVY6</accession>
    <name>A0A8J4PVY6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitinyl hydrolase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_002652</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000075">
        <property type="protein sequence ID" value="KAF2076038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVY6"/>
    <dbReference type="OrthoDB" id="29137at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2230.10">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035927">
        <property type="entry name" value="DUSP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006615">
        <property type="entry name" value="Pept_C19_DUSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF21">
        <property type="entry name" value="PEPTIDASE C19 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06337">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00695">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143791">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51283">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUSP" evidence="3">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="2">
        <location>
            <begin position="350"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="699"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1008"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="327"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="700"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="747"/>
            <end position="759"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2076038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1033" mass="117078" checksum="41DC935707245327" modified="2022-05-25" version="1">MMRDEKFEQKKIIKENWSHELVAGESYNIISTNWLKQWMAYVNFNDQTPNGINGSSNHHHTPSSSSSSSHTTIDQDNSSNSNNNPSDKPESIYNRDLIEGIDSEGEFIISKKVLEKDDYEIIPNRVWTLLEGWYKGGPEIKRKCIELGLRNEKIVEVKPMILNIKYSQSLYNLLNINKSSSSSSSTATVQQQFKDLPSPPQNGYDFIYQKSRKSTLLEFKEYICKKENIDSTKLIIYNNNPQKSKPIKKYFETLEDLKFESVNNLYLDMDPSSSKDHKFFGKVKKIFKKSSSSSTSSSSSSSSKGSVSSATNGHHAETNGHNHKDNASSSENSPSYSSNGKEIVLHKSVCGLTNLGNTCFMNSSLQCLAHTTPLIEYFLSGKYINDINKTNPLGMKGQIAEIYGKLMKDMWSGTPCVSPKHLKWIIGKYAPQFCGISQQDSQELLSFLLDGLHEDLNRVQKKHYQEEKDENKETRADSIVATEQWDNHLKRNQSIIVNEFQGQYKSTLVCAKCSKVSITFDPYMFVTLPIPVPTERVFEVLLFRNKPDAGGIGPDPNGVYCFNNAMAPIRFCLKLHKREDVEALRSGLSKLTGIQSSCIALGETFKNKISTFLNDQRSLMSIKDRDITIAYELPIAGDDISRIHVLHKRRGELILLPYVLLLKYSETTTKDLYRMIWERIGHRVKKGWKQAVKKTISKENISNSSSSSNSSNNNNNNSTPIISSSTTANQKDEESAISSPTDISQFGEEDDTSSVTDDESFENTVYPFILRTTNAYGMDCDRCKDCKGCIIKCDDSLLTSLYKNPKYWRDGCSNVTLDWRPEALEFFDATELEDPNSQFTIQHQTTELKTEFKSEINLNDCFSLFTKNEKLGPNDTWYCPQCKAHIEGSMKKLELWSAPKILVIHLKRFHFVHRHEKITAFVDFPLDNLDISKWVLNKNGGPYNYQLYAVSNHMGGMGAGHYTASVKNRDQWFLISDSSYHSIEKSKVKSSDAYVLFYELKPQENANITNSTLNNNHATTTTTTTTTTSTSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWU7</accession>
    <name>A0A8J4PWU7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F5/8 type C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_005319</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000208">
        <property type="protein sequence ID" value="KAF2073374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWU7"/>
    <dbReference type="OrthoDB" id="5985199at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045335">
        <property type="term" value="C:phagocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098636">
        <property type="term" value="C:protein complex involved in cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046871">
        <property type="term" value="F:N-acetylgalactosamine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070492">
        <property type="term" value="F:oligosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000016">
        <property type="entry name" value="Contactin-associated protein-like 4 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2080:FF:000001">
        <property type="entry name" value="Discoidin-1 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052487">
        <property type="entry name" value="Galactose-binding_lectin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037221">
        <property type="entry name" value="H-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019019">
        <property type="entry name" value="H-type_lectin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46938">
        <property type="entry name" value="DISCOIDIN-1 SUBUNIT A-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46938:SF2">
        <property type="entry name" value="DISCOIDIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09458">
        <property type="entry name" value="H_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141086">
        <property type="entry name" value="Agglutinin HPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01285">
        <property type="entry name" value="FA58C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F5/8 type C" evidence="3">
        <location>
            <begin position="11"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2073374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="255" mass="28303" checksum="FEE87FB4A1A5FAA5" modified="2022-05-25" version="1">MSTVPANSVACIANCLLQLRSSTDYNADHGAKNSLLNFANSTTDKNFTVSNSWSSAGLDKNQFIMAGSDAVKEFVAISTQGRGDNDQWVTSYIIRYSLDNINWFEYNNSQVFNANTDRNSIVTHTFNPPIKARSIAVHPQSYHISLRWEVYSKPLDTTNHNSFVQVGTVSIGDRSLNQGNGDRKITRKVVFPKAFGSIPKVAIGSHLIDAQTDNGQMRWRVDAANITNTGFDCVFNTWGSNCVYDIIVDYIALEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXB5</accession>
    <name>A0A8J4PXB5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_003686</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="AJWJ01000119">
        <property type="protein sequence ID" value="KAF2074990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXB5"/>
    <dbReference type="OrthoDB" id="9332038at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000078">
        <property type="entry name" value="Serine/threonine-protein kinase PRP4 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF103">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PRP4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="469"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="394"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="14"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="95"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="225"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="283"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="364"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="498"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2074990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="788" mass="91487" checksum="349D7358715C075E" modified="2022-05-25" version="1">MSLDDTKSNISLDNNEKKRKEIDNLVGEKQDESKKLKIESSAIEDGEVEDGSITPPPLPSKTLLNSGKDISHSPLSPSSSSSQNKNISNITSPKKNYDRDNSSRNNSNRDYDRERDYQKRDYRDNSSRNSRDYEREKDNDRDRDYNNSRRGDRDYNRRDSRDSRDSRDSRDSRDSRDSRDYKDSRDSRDSREYRDDRSKNDRDYKSNNNNSKAIEKVTISSNNNNDKKDTSSPKENYEKSQHDNSKEKKIDLSPVTITPQAKIDNNSNPINQDESLIIDEEDKEAKEKRLLEESRLRRQRIKEKYEEEQKLKQQGQQPQEQQPAIINQQQQKDVKIEEEPKDDDENNTFEWRKGDDNTSNNINNEEDQDSCSSEEDLKKRGIIDEKDLKHKLKESTTKSTTTDNKSKKKEEEKDDDDILDMFSDSPENGLEISIVNNSKTIYSSINLTDNWDDADGYYKYRVGEIINQYQVVLPIGSGVFSTVVSAKNTKTNEDVVIKIIRNRPSMNRSGLKEIDILTKINSNNNHHQKTHCIQMKDSFTFRNHLCIVFEPMSMSLHQLIKKFGKNIGLSLNAVRVYAKQLFIALKHIKNAKIIHADIKPDNIVVNESKNTIKIIDFGSAGETHESEITPYLVSRFYRAPEIILGNKYDYAIDVWSVGCCLAELFTGKFLFPGRTNNDMIRLFLEYKGPFPRKMLKKSQFLANHFNDSFVFLKQENDVVSKSIQRVPYEIHKPVKDFMNFLMPKNLTLPESDLKKLVQLKDLIEKCTILDPEKRITPSEALNHDFLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXG6</accession>
    <name>A0A8J4PXG6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7035 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002901</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000085">
        <property type="protein sequence ID" value="KAF2075818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXG6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF29">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035293046" description="DUF7035 domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="2">
        <location>
            <begin position="613"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="686" mass="76402" checksum="1ADFE39942A2FE32" modified="2022-05-25" version="1" fragment="single">MRANIYLFLNVLECIQLGTIVKPTNLDEYGYSGCQYVYTLILIDAVNTITILSPTTSQFISNLDGNQKFTITFNQVFGDRGTFSLTAVANQVETTFTLDDFECLEIPPITMSLNQELVYSTNYQFFTWVAKIETNPPMTKHLPGKSCNMNLVSGPLAFYPQYYPINICSAFMTISSIQLNTPTIVNFNPSISQNVNIELNTPYTKDPFEGSIDAIASVGTKSFNFIMNYKDNVNMNPFFLLLRQLPTVQGNQVNGTFIFFINYKGGYYYDFDIDSDYSFSVFENNNQVDKVTLHGTSVKGSQVVYLTDTSGVAAVPSDPNAAYLKMVYTMRSSFYDPRNYNTIVLTNILSKTYVSFPFGYTSGNIVAGYTMSFSYKSSSHFGYNNFKTRLAANAPKTNTNTLPVSFNTQPPIVESFDLIGRGVGKGVYRIIVSDSIELIYGILSNYDESAIVLMGTESVVASDGGHNVTFEFLLNSLSYDLFVYSHVGLYQIVTPPPLGGLFDLQNLRNIVFLVNDVDTTNKVTNNTMMVYYSDPGFIIRLARSTSAGQDIMYFDSVYDTATQAYIIPFIIPPNRMTGNYNYIINVGNPGISCPSEIFDVLFEPLRVRSDNYDDIPPLITAMSVLPSSIVSSVDDFTELAFVVTISDTYNGLLNGSFWITSELDPYGYNITIDPSMADSGDQYLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYE8</accession>
    <name>A0A8J4PYE8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sodium/hydrogen exchanger</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_002204</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000059">
        <property type="protein sequence ID" value="KAF2076463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYE8"/>
    <dbReference type="OrthoDB" id="196264at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098719">
        <property type="term" value="P:sodium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004709">
        <property type="entry name" value="NaH_exchanger"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00840">
        <property type="entry name" value="b_cpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF187">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01084">
        <property type="entry name" value="NAHEXCHNGR"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0050">Antiport</keyword>
    <keyword evidence="6 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0915">Sodium</keyword>
    <keyword evidence="8 9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035161013" description="Sodium/hydrogen exchanger" evidence="12">
        <location>
            <begin position="26"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="71"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="108"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="136"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="267"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="354"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="427"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="13">
        <location>
            <begin position="58"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="495"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2076463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="536" mass="60072" checksum="A0F4CD8C67F6A9E2" modified="2022-05-25" version="1">MKYSFLFSLFSVILLSSFFVEQTLAAHDINHGCKDIIGCGPPESKQVLIFVVLLFFSVMLVYFISSLNIHYLPESIAIVVYGVVFGVIDKYTSSHIMDNVSLFDSDKFFLFLLPIIIFETGFSLPQSDFFRNLLPILMLAIFGTLISFILTGFGVYLMGFLGVSLPISLREGLILGSVGCSTDPVATLAIFKALDVEPLLFILVLGESILNDAISIIAFKSVLTYGVSEIWKPVLIFFGVVFGSVFIGVATILILSITLKLFDIGKYPAVETIFIIIFSFISYHISDSIGLSGIVAAFFSGMTMSHYGYRGLSEETKHTISQLYRTGAFIGETIAFIYIGISLPIHEFHVNVPMIAWTLFFFLFSRAFSIFPIFFILNNLKLKKQPYVSLAIQTVIWFSGLRGAISFSLSLSPALDDFPNGKHIQTTILICVYITLFVFGMGTYPLLKILKIKTNQREQTLESINTMHIDDDTKIGKLGSMIRTIDDKITQYFSRPAPPPPLLREEEEEDEKISSDEENSHTNFEYYQTVKLDNDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYT6</accession>
    <name>A0A8J4PYT6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA (guanine(46)-N(7))-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_000989</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42708"/>
            <dbReference type="Rhea" id="RHEA-COMP:10188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10189"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74480"/>
            <dbReference type="EC" id="2.1.1.33"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.33" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000022">
        <property type="protein sequence ID" value="KAF2077673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYT6"/>
    <dbReference type="OrthoDB" id="42736at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043527">
        <property type="term" value="C:tRNA methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008176">
        <property type="term" value="F:tRNA (guanine(46)-N7)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033443">
        <property type="entry name" value="PROP1-like_PPR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003358">
        <property type="entry name" value="tRNA_(Gua-N-7)_MeTrfase_Trmb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417">
        <property type="entry name" value="3-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417:SF14">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02390">
        <property type="entry name" value="Methyltransf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17177">
        <property type="entry name" value="PPR_long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51625">
        <property type="entry name" value="SAM_MT_TRMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="PROP1-like PPR" evidence="10">
        <location>
            <begin position="282"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="8">
        <location>
            <begin position="318"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="8">
        <location>
            <begin position="388"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="112"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="133"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="177"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="223"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="245"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2077673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="784" mass="89329" checksum="66A55D990018E1E4" modified="2022-05-25" version="1">MMDLKRKFESIGSASSTKLLVKNIPKSLTKNDAIDRLKKIIQNPKVSITIPIVTPPDVAAKLEPETTIAFLTCTDIALYPAIKSLVNGKLVSDKKLVVDFYQSKPIAPVAKPVAQSTPVAVVKQEQKEKVDSNNKNNSTSNSNNTTETAAKKEKPVKKQKVNNDDTKPTTTSSNDQSEVKEKVSDQQQQDKSKKPNPSKEKNDKQQQTKDDNNDNSNSSVDNNKQDNKENNNENKKQQQQQQQVKKQENKKKENEKKEQQKQEGEQQIEEIKREPTVRNNRAVVTKITQQLVSYAEVKDFVQLTKSYNYLKKIGAKPDHITYGVMLNACVRCQEYKKAREVFQDALEADNTNEIIYTTFLKALCEINIKEAKEFIDNMLKPEAKIAPNLRTFNAVLRACMRTGDVEIAQVIIEAMKKVNIQPDSTSIEYVMKMYSHHLMIPAAWELLASVFEEMDNQLSPIIFSRLALASLLRGDIKSAVKALSITDNILTKAPQAIQNSVNHKNKGKLSDKNKISSSLFERISRQEINEECDRVRSHLHKLTDAKFKSKITNMESFNRIFFIPHTFEHALARTVPAAMIEDKPIGHSNLLSLFNSYKPDGSNSNSNRQLKMEICSGHGHWVTERAGQDLNADWISLEIRYDRIFQIWSKMILESIDNLYVVGGDAMKSLRKVVPSDILDEVYINYPNPPVWSGADRLIDEKFLKEVNRVLKKDGTLTIVTDDKPYSDQIIQSLTKSKKLAKIFKPVKDNYLINLSEDYGYSYFNKLWNNGQRIKRYCIYITKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZB9</accession>
    <name>A0A8J4PZB9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002936</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000087">
        <property type="protein sequence ID" value="KAF2075754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZB9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2075754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="251" mass="28766" checksum="DDC90D1A8D33C3E8" modified="2022-05-25" version="1">MGNTSSAPKLVGSKRIGIHRSTSFAKNSTNPSGNIFDQETVFPISKVRKIVEKPDNLLKSANNNPNKVGRLYLCKKDNGLIHSINFKSTYHFSLVLDIGGHSDEHTLRGALMHIYFSSDKRIIYSQRPIEYIKLSYNSVEDAGTLVYPYDNIERLQTDYPEYIEMDMLSDKNHLWYYYILIQMDNWLKEFIEGHGSVFSIHCNCQSFAKYLCAKLSGVQFPCPSIFGERSIDKVVYTSYSVIETSFLKKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q003</accession>
    <name>A0A8J4Q003_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Inositolphosphotransferase Aur1/Ipt1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_000361</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000006">
        <property type="protein sequence ID" value="KAF2078378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q003"/>
    <dbReference type="OrthoDB" id="17559at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026841">
        <property type="entry name" value="Aur1/Ipt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052185">
        <property type="entry name" value="IPC_Synthase-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310:SF7">
        <property type="entry name" value="PA-PHOSPHATASE RELATED-FAMILY PROTEIN DDB_G0268928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14378">
        <property type="entry name" value="PAP2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="290"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="329"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="413"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="439"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Inositolphosphotransferase Aur1/Ipt1" evidence="6">
        <location>
            <begin position="327"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2078378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="487" mass="54678" checksum="4D13412A633AC6C3" modified="2022-05-25" version="1">MGVPQAIEIQNQSSTDFKYFHLNEDSEVDIGGDGPKEMGGVGMMPKLSVWGWFTPSQYHYKKGLQDSSTTIGEVDMHSLKHKSIGFHSHQPFLQSDGGSDTEDNGKDFKIDIHPSSDSMMMEEDTEYAKVYNGANLSTNNKGPIMYPYSPVYSDIAFSVLVGVVCLFSIIYSLLVGPLAIFFAFAVSIIILVSYISTSFAKNNKWYLYSITVLSVGLGLTIPSFFQATGAVVLGTNRLTWDRELASADSSLLGWIFPLGQMGLFIDKSSFIGPYSFIGELSTEIFQLSYISYYIWGYFMEVWILFNLWKCYLSKDAAQMRMMPIWDQRLKMFICSWISTYFVVFSINLIFPAVSPRVFLNDQYENKLTGFGFAGFVRDKIENAAKGSFGSFPSGHIATSWAISFASYPISRTYAFVSGVAAFLITIATMYLRYHYFVDFLAAIPVIIICLLFGGFYSVSSIKQSLLNLFVSIKKFFKTTIAKFNKSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0P5</accession>
    <name>A0A8J4Q0P5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Band 7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_002053</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000054">
        <property type="protein sequence ID" value="KAF2076624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0P5"/>
    <dbReference type="OrthoDB" id="2105077at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000004">
        <property type="entry name" value="Putative membrane protease family, stomatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2090">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043202">
        <property type="entry name" value="Band-7_stomatin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264:SF19">
        <property type="entry name" value="AT06885P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264">
        <property type="entry name" value="BAND 7 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Band 7" evidence="3">
        <location>
            <begin position="39"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="439"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="601"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="662"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="441"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="455"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="601"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="614"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="662"/>
            <end position="713"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2076624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="784" mass="89144" checksum="F381E4D5500A2C48" modified="2022-05-25" version="1">MSVLAQSTFKRAVSMLKKPSFNISALNTRNIHNNESKRGFFTIINQYEMGVTFTLGKVTSVKKPGIRLQIPFIQSIELVDTRIVSFSLEKQEIISKDNISLIADAIVYYRVVDPLKAVCEVTDHDRVIRELAQIKIRDILSQNTLDDVLHNRDKFSEEIFEDVKSSAESWGVEVQRINLKDIKFEEGMARAFAKKAEAERLREATIINAEAEVLVSKKILEAAKTLESSPVAVRLRELDALLQISKEPSKSYIFVPTGLFGSTAQSEKPRWIENAFISGTKPEDPCGQVISMDEHNEKKESYSFYLSDNQSYLKLNIVPNEIKSMMKERKFSNPAQFFGAILCLHEYSFNDFCIDVKRCSLVKKCKLSSIKKLLPLSEQPRVIALLAERNSILPRTKSLPILDSEKLRDLGFLEQGTEKREFKKLPGLVLNKNPIKKEQEQIPNQLPLPTSPLQKQKDQEQQEQEQKKQEEEKKLKAKIFEERFTNISALEAIISDDQLEILDSLEGWSLKAPEFSSQSAILTQFTPTKSQKPKNTSNVGKVLDFEDITDFSFSFSPSLPSAVKKRKRQPLENESDESIIFNLQDVFESDAKDLKIDKQDNILNNNNNNNNNIDNDDDEDPFGSDDFAPDYRQFSDNNEFEIDNPVLFDDIKVKSERTNIVNNSNKNKITNNDNENNNNSNNNNNNNNNNNNNNNNSQIKNNEASNSNNSSFSFGDSPISKLPTQAIGVGEQKPTTPSNVLKFDEIGVDIECHFPQKSTIPNQQKCQMKKYGDYTIIVMLDSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0S1</accession>
    <name>A0A8J4Q0S1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Arp2/3 complex 34 kDa subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002713</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Arp2/3 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
            <location evidence="1 6">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ARPC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000078">
        <property type="protein sequence ID" value="KAF2075957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0S1"/>
    <dbReference type="OrthoDB" id="148331at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1460.20:FF:000009">
        <property type="entry name" value="Arp2/3 complex 34 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007188">
        <property type="entry name" value="ARPC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034666">
        <property type="entry name" value="ARPC2/4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12058:SF0">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2_3 COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12058">
        <property type="entry name" value="ARP2/3 COMPLEX 34 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04045">
        <property type="entry name" value="P34-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69645">
        <property type="entry name" value="Arp2/3 complex subunits"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="292" mass="32883" checksum="76C400B1229C529C" modified="2022-05-25" version="1">MLLLETHNRILYDEVVAYFNSDRRTDNIFADFDGVKFHTFTSDDKSTLTVSISIKGASDLLKQGGSALLKSVYGDLFQAKPEGGYDVTLIIPSTYSGNKDELAKKVGLLKRNLVAAPFLMVFEGVESKKALPDIITIDYRSDESFYIKPQGDNVIVVFDITFKDADDVVLSKIFLQSFVDARKTMNNTPSVTFSQKDPPLELKGVKGVRTGAHHGFVSFVLFPAHLKKPQETADLIQTFRDYLHYHIKCAKAYMHTSMRNRVESLIQVLNRAKPEPVTTVKRTITGKFFKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0S8</accession>
    <name>A0A8J4Q0S8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histone acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_002728</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MYST (SAS/MOZ) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000078">
        <property type="protein sequence ID" value="KAF2075972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0S8"/>
    <dbReference type="OrthoDB" id="787137at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000022">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000155">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.60:FF:000001">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000002">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.60">
        <property type="entry name" value="N-acetyl transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002717">
        <property type="entry name" value="HAT_MYST-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050603">
        <property type="entry name" value="MYST_HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025995">
        <property type="entry name" value="Tudor-knot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040706">
        <property type="entry name" value="Zf-MYST"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615:SF219">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE KAT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01853">
        <property type="entry name" value="MOZ_SAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11717">
        <property type="entry name" value="Tudor-knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17772">
        <property type="entry name" value="zf-MYST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51726">
        <property type="entry name" value="MYST_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYST-type HAT" evidence="15">
        <location>
            <begin position="200"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="13"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="13">
        <location>
            <position position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602717-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2075972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="480" mass="55372" checksum="F9854C8AF8E36DC7" modified="2022-05-25" version="1">MTDNTTPAVQNKDSSSTNGNGSDSNSSSTPSPPTADTSIAPESNNSTPQTNSNGNTNNANNNNSSPNLTSTTPPTTGAKKILENGTHIYCKWRDDAYHKCEIIDKREAETETGTYEYYIHYHEFNRRLDEWAHESRFDFNRIEMENKPHAPLAEIQNSEGQNRKITRQIKRKYDEMNHIQKGAEDESKLSAIEKEHEEITKVKNINVIELGRYEIDTWYFSPYPEEFAKCDKLFLCEFCLKYMKKKKTLNRHKLKCDLRHPPGNEIYRNGNLSVFEVDGKKNKIYCQNLCLLAKLFLDHKTLYYDVEPFLFYVMTECDQRGCHMVGYFSKEKDSPDGYNLACILTLPPFQRKGFGKLLISFSYELSKKESKIGTPEKPLSDLGLLSFRSYWTQVLLEILRKHKGNLSILDISNMTSIRTEDVISTLQSLNLIRYWKGQHIISVTPKAIEEHLKVYSKQTTKIEPKCIHWAPLNPPAKKHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q131</accession>
    <name>A0A8J4Q131_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cationic amino acid transporter C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_000661</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000013">
        <property type="protein sequence ID" value="KAF2078023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q131"/>
    <dbReference type="OrthoDB" id="5982228at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029485">
        <property type="entry name" value="CAT_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243:SF4">
        <property type="entry name" value="CATIONIC AMINO ACID TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER YGJI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13906">
        <property type="entry name" value="AA_permease_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="297"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="341"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="393"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="421"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="460"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="488"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="529"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="553"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="Cationic amino acid transporter C-terminal" evidence="8">
        <location>
            <begin position="526"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="596"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="671"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="601"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="620"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="683"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="711"/>
            <end position="722"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2078023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="722" mass="79390" checksum="A7CF0A210F50E61C" modified="2022-05-25" version="1">MDNIRYRLAKFGQTSSRKKSIFQLKYELENDSYQTTEHIPKVSGDDGEPVDNSVNLENQTRGGMKKCLNVTDLLAFGVGAIIGAGIFVLTGVAAHDKAGPAVLISYLISGFACALSGLCYAEFASRVPCSGSTYSYSYIVVGELVAWIIGWDLTLEYMIASATVARGWSGYLKSIIEAGGGYLPHPFAPIHLAQGFSLDLIAFFSVIALTLIIAFGMKESARFNKIFVVIKIAIVLFVIVVGSIYADTNNWKPFNPYGTRGIFGAAAITFFAYLGFDGVCNVAEEVKNPQRDIPIGILGSLGISTVLYVGVAVVLTLMVPYDMIDVDAPLSRAFQNLDLKWAQIIVAIGAFSGLTTAQLSGLISQPRLYYSLSRDGLLPKWFSYIHPKYQTPFYATLFTGACCAVIGMFVEISILADMVSIGTLLSFTLVSTCILILRYPVSHSASLSNSKYPVNRFPLFLQNPAYVVCFIIVFAAVTSASYVYSLSWVITLVFGLFGLAFSVIPFFFKETEENESTSINASKKSFKCPWVPLIPVVSVWANTYLMLSLSWQTWVRLVVWLIIGLLIYVFYGQKNSKLGKEQELDNFNLKYQNEELDSAEESNTNGNDENATNDSTNDNNHLKIPDSEKQPLSPKMASSPLSKIKFKTTPNGLYGQLPEDVYVEPISLSNIPIEDSNNRNVGDDDDNNDNNDNNDQDEKPSSSDSSEGESGDLKKEKDELIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q186</accession>
    <name>A0A8J4Q186_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Actin binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_006043</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the formin homology family. Diaphanous subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000261">
        <property type="protein sequence ID" value="KAF2072641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q186"/>
    <dbReference type="OrthoDB" id="410721at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045010">
        <property type="term" value="P:actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.150">
        <property type="entry name" value="Formin, FH3 diaphanous domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010472">
        <property type="entry name" value="FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014768">
        <property type="entry name" value="GBD/FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010473">
        <property type="entry name" value="GTPase-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46345:SF8">
        <property type="entry name" value="FORMIN 3, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46345">
        <property type="entry name" value="INVERTED FORMIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06367">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06371">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01139">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01140">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51232">
        <property type="entry name" value="GBD_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBD/FH3" evidence="6">
        <location>
            <begin position="19"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="FH2" evidence="7">
        <location>
            <begin position="655"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="482"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1040"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1095"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="404"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="522"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="536"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="562"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="587"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1040"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1066"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1111"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1137"/>
            <end position="1161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2072641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1188" mass="131694" checksum="1C1A4050584B8ACA" modified="2022-05-25" version="1">MRLFARKKKDKDGNDSKETSENEFLSSLPAKANKRYSMAYSSFSPPGTTSNSFKSMNPDSYSPSLTGLKIDDKGKAVDTPEFFVNTISVLPNIEILASLCSTLQTKPSSWSKALIEANGIEVLLDVLVHIEKGGQKDSDIILQSLTVTCLLSLLNSKFGIEKAITTPSSMNKLILCMDTGKVDTRSSVFEILTAVCLISEKGYQLVLDAMTHFKQSRKEKFRFTFLVESMKKLADDKEYLTTCLALINGLVNSSDEIDERVQLRTEFSRLGLDNIIKENKKIPYEECPDLLTQIDVYEDEARNDQEELTEQFSDLHFNINDPKEVFTAIMEQVAKRPLHHPFLHILQCLLSLPSDSDAGMLSWYSIEKLVQQISVNKQMIGDNDYSGHAEKIGLEDLLASSAPSVAMQTEYKAALEELQKVKDQLKKANFDLNIANTELSSRSQESTVLKSNMFNTVKLKDQEIQKLKQTVRKMDAAFFQAPTSDEIESSTNSGRGKMDSNFFSPPPATGADKPPVAPSHPPSDKPPLKSALKSDKKVKKEKSEKTKKKKSDKTKATDDESTTVVTDVNSKNSNGTTNGSESSSTAPAPPAPPAPAPPPPPMMGGPPPPPPPMGGPPPPPPMMGGPPPPPMMGGPPPPSGGLFGGGNKPPANAPKFQLVKPTTKVKQLQWTKLPNRKLNDTIFTNLGNIKTDWLNLDEIESLFFSPDAAPKKLDASDKKSTSNTKPGTVTVIDGKKSQNLAIYLSKFKCTIEEMKNGICMLDESLFNIETLKALETYLPTEEDMEAIRDYLKNGELKMLTKAEHFLLDLDSIPNIQERVKAFSLKLCFPDKLKEIKPDLELFTKSIHQLKSSKKFLKVIEVILIIGNFLNGGTARGDCLGFKMDTLLKLTDTKTFNNKSNLLVYIISEIEQKFPECLNFLDDLDLVNEASKISLAQISGELNLLKKDLESVTNGVGKMKQNREDMYFFSSMEDFIKDAGIELKIAQEQYAEAESEFQKLAVWFGEETKIPSEELFSVLTRFLQTFDKSFKDFQRDKESAERAMKRDQMKAQKTAALKKISGKASAGKNSPLNSSTSSMAEGMVDDIMQSVRDGDAFKQRRQKKTTTGPSITVTDSDTTATETNGKESTSSGRRLKRSDKSDKSDKSSSSPKEEDTENKKDVNVAAKALTVVMRARQNYSRIDQFDFDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q196</accession>
    <name>A0A8J4Q196_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NAD+ kinase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_000584</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000011">
        <property type="protein sequence ID" value="KAF2078113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q196"/>
    <dbReference type="OrthoDB" id="24581at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.30:FF:000009">
        <property type="entry name" value="Poly(P)/ATP NAD kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF0">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2078113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="441" mass="48957" checksum="6C095694B4A1E319" modified="2022-05-25" version="1">MIKNIIFNRYTCNRAISNYSSWLLINNNSNSSRGHINFENNIFNPSNNRSISSSSSNTNNSQSNNNIDSRLITPLQLLSNKVEYKIVRETNNPNSSQQQEGSRTRFKWIQKPKTVLIIKKHKDKKTTTFLNTIASFLNKEYGMKVLVEPNAINGTDPSCVETYSEVDGHLLGKVVDFVITLGGDGTLLHVSSLFKEEVPPVISFHMGTLGFLMPFSIDEFKESLSNVIKGDFLCTNRMRLTCDVVSEQPKLINNNNSNNNNNHSNIDQSQQLITKTFQVLNEVTLHRGSNPHLTTIKCTVNGHNLSDFIGDGLIIATATGSTAYSLSCGGPMVHPCINCILLTPIASSSFSSKPALLPDDSVLKLNMISQKGRTISATFDGTRSVKIEQGDYLVIRKSTHPFLTINKINETTDWVHGNGKLVEMSEKSSIWNQSVYTTSAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1W8</accession>
    <name>A0A8J4Q1W8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pirin family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_001466</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the pirin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000035">
        <property type="protein sequence ID" value="KAF2077212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1W8"/>
    <dbReference type="OrthoDB" id="198735at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02247">
        <property type="entry name" value="cupin_pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012093">
        <property type="entry name" value="Pirin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008778">
        <property type="entry name" value="Pirin_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003829">
        <property type="entry name" value="Pirin_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903:SF8">
        <property type="entry name" value="PIRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903">
        <property type="entry name" value="PIRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02678">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05726">
        <property type="entry name" value="Pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006232">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pirin N-terminal" evidence="3">
        <location>
            <begin position="41"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Pirin C-terminal" evidence="4">
        <location>
            <begin position="192"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2077212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="34151" checksum="6CF68E8CBB832F28" modified="2022-05-25" version="1">MNNNNNNNNNNNNNNSRVLSNIYEGRCQEDGDGIQLIRTIGSTIRSVDPFILFDEFIKDNSIDDYISGFKSTPHRGYSIVKILLEGNDIEYKDNKGNCGILKAGSIQWMNAGRGLIHSSRLKQEHQIKNHFFELWVNLPNHFKMGEPSYQNFQENDIPRVKQENGGQVIVFAGCFKDQIGPLNDPVSNPIFLDVFLPPNSSLNLPFPVDNSTIVYVYQGSAKFGPKDFEKQVFESQLGVFSREKDQTHIDVYTNKDICVRFFIASCTPINEEMVRQGPFIMNTEAEIDQAFYDYYNGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q231</accession>
    <name>A0A8J4Q231_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000817</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000017">
        <property type="protein sequence ID" value="KAF2077855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q231"/>
    <dbReference type="OrthoDB" id="10373474at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37508">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37508:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="178" mass="20280" checksum="36ECD8986D744B17" modified="2022-05-25" version="1">MKYLQLYRQYQDLHRKSNADLATSIVKLKSLNDNGNYLQVSLISLDIVKKTMGKVKTTFENTALFWRQVAKHCSRLSSIENVEDTRDCFKKDPSFFNDFVDAIKASGLEWLSLAHINHIAATSILQAKDNVDSVMNDLPDKSEAQKIIQSCTKEIQQMVLEENVKLDKGSVLQIKDAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2S2</accession>
    <name>A0A8J4Q2S2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Essential protein Yae1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005586</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LTO1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000227">
        <property type="protein sequence ID" value="KAF2073091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2S2"/>
    <dbReference type="OrthoDB" id="48036at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019191">
        <property type="entry name" value="Essential_protein_Yae1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052436">
        <property type="entry name" value="LTO1_adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28532">
        <property type="entry name" value="GEO13458P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28532:SF1">
        <property type="entry name" value="ORAL CANCER OVEREXPRESSED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09811">
        <property type="entry name" value="Yae1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Essential protein Yae1 N-terminal" evidence="2">
        <location>
            <begin position="20"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="129" mass="14894" checksum="02B1D17467CDC9E2" modified="2022-05-25" version="1">MDEFDQALNIESDTYISSKEEGISDGKRLGYIDGYQLGFEKGTELGQEIGYYQSCVSVWNNLVNIYKSTQKFTPRSLQNLEKLTKLLDNYHLNFNDENIMSSLNEIRVKFKLTSTQLGLQTKEQNELSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3A2</accession>
    <name>A0A8J4Q3A2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Bidirectional sugar transporter SWEET</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_000615</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SWEET sugar transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000012">
        <property type="protein sequence ID" value="KAF2078064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3A2"/>
    <dbReference type="OrthoDB" id="409725at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047664">
        <property type="entry name" value="SWEET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004316">
        <property type="entry name" value="SWEET_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791:SF31">
        <property type="entry name" value="B BOX-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791">
        <property type="entry name" value="RAG1-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03083">
        <property type="entry name" value="MtN3_slv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="110"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="250"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="250"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2078064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="300" mass="33674" checksum="2FA2B8A0B0961C5F" modified="2022-05-25" version="1">MSTSSAEVVNDEGNHQAILATQILGNVFVALIVISAMPTFWKMDRTRNVGNFNIYPFIAMVGQNIMWVVYGTVCKVEGLVYVNAFGMFINLLHVMVFLSVCGEVSQKRKVILATFIDVTVMGVFTLVVVWLAPEDKFNSIFGWFCSILLVVFFMSPCYFFYSMYKARSVDSLSIPLLITSIIAGVAFGMYGVLLKDWFVTVSNFSGTLSGVLQLLFFVIMKVFIIKPEDMVKKDDIIEYQEEIKLEMRKEVKGKDDDDNQDKETDNENDLDDKGHVQEEESIASEQGHQTIDVGGDSGSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3B3</accession>
    <name>A0A8J4Q3B3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DOCK family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_005337</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the DOCK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000210">
        <property type="protein sequence ID" value="KAF2073346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="47328at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027007">
        <property type="entry name" value="C2_DOCK-type_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043161">
        <property type="entry name" value="DOCK_C_lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043162">
        <property type="entry name" value="DOCK_C_lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027357">
        <property type="entry name" value="DOCKER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046769">
        <property type="entry name" value="DOCKER_Lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046770">
        <property type="entry name" value="DOCKER_Lobe_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046773">
        <property type="entry name" value="DOCKER_Lobe_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317:SF80">
        <property type="entry name" value="DOCK FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06920">
        <property type="entry name" value="DHR-2_Lobe_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20422">
        <property type="entry name" value="DHR-2_Lobe_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20421">
        <property type="entry name" value="DHR-2_Lobe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14429">
        <property type="entry name" value="DOCK-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51650">
        <property type="entry name" value="C2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51651">
        <property type="entry name" value="DOCKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 DOCK-type" evidence="5">
        <location>
            <begin position="589"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="domain" description="DOCKER" evidence="6">
        <location>
            <begin position="1525"/>
            <end position="1964"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="548"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1135"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="16"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="66"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="549"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1164"/>
            <end position="1193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2073346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1980" mass="224218" checksum="F71F2F83B437D686" modified="2022-05-25" version="1">MDVSTGNNSNTLDVKSNLATSSSAAFTNNSSSTLSPSTKTPSQNNIQNNSSNISVSQSPMSQSYNPSPKQPASPSIHNSAQATTPTTTGVIAKSPSASSGATLHNVEYKFEEYEELFFAKQIINDPLKISVIKDLLSIQNQKRTTNIVGSNVPEQVLSEHDKLPQGIQESINIFKQEWTVVNRDLPPPLTSTPFKVLPIQTYTCDGNFDESSAEEAVKKLTDELIPNPPIPTPDPKSVEFMKTQRVPLFQTYYINDDLPTIELPRVGLPLTENVGFQFIVECTQFKPTLGEFEPFFGRMFLFDANPESKEGVVITEEFHFDLGKNLELLPKPNPSKSMEEIEAPTKIQKCLFNLNYRSSDIYLVICFDKVILGDPEETTKPYFNTPGKPKEIQKFQNEVTAGLSRLGNFKQPFVWGCVELFDSNKKFIFDQPDNDLKLTYFSKSSKSPSDLLTFITKDMKKQHTSKSENLFDCNLKITPLATNNHVIHSSTNGTTAATSEEKYRYNETVQGRIDYLLRPKEPYHPGGDGQPSAPLVREVIQFDEELAVNTENGGTNESNSSPDNIKDSMSNLSAPLSTKETTEPFLTYTNLLYFYPKSVNLTNYKSDKGSSARNIFLEIKLLEDDTNVNNRGLKSIHGTSTTPILTKSLYTSVVYHNKKPKFEDEIKINLPPNLTPNHHLLITFYHLGCRQSKKSDKPETALGYCAIRLFDNQQIILDGKHKKPVATVFPPKYLAQEARDPKDPSANLKFWVDNKRPVFSFKTRVISSLYPQDPVLSALLRDSLDGGDQATLTDNFKKLSTVSAKYKTQFFPAIIRVLFKVIVTQSLPIETVSAAFNALLQLVDSVPEDILSQYITYVFNSESVSLTLYDSLIQTWNHLLESSEQSQILLSIQYSWFLFGIIRKSMIADVNKKGDLNTGRNRTPRFQEEFLNRLRHLFDQLLMHLKQSTKLLLAKKFIVNIAFFINDLLDIMNRGFIFKLIYNYVTGLDTSNNSMEMTELKSRFFRVLSLSDSFIALNLPNTFTFPPLADVYTTFYKKHFIIGLLLQEVSACLAAKEKDLRLKMVHTLREIMAKYDLNPLYNSQSMRERICSLYFPYVLIVVNAYDQISRFSADEQRSCALGLFLHKNPKIVEEETLSPTPLPPHIITPDSTDSPKRKDKKTPKNNSNGGPNVSQKEGNASISSSGNSVTLASPANRDKTKALIEQSMSSSMGGSQKAERTYSICQQQASTLGTSLGSSSATTAAPGGSSPLPGLRSFANSMTIDPSLTLFGNDIVTLMNNNLTCEVSLTVLNCLIVFIKENKQELLKNQQSTFVDMIFKVILKLIKQEQAHSITKVMFTVLSSLVSEFRTTIFKMNNSICSELTQAIFDYCSSKHAPDRQMGTTLIVLFIQNNLKEMGHFSRMKLQSTVSISEILNNREKENEAKKKKHISVNAEEEKEFYNYLYADLESITKYVKQLCNNSLLKSSVVTNSKFSKPARPQAPIAQQIDELKQRLIGVIHNNVQIQQYSYDPEMKADLYYSLSTTFIESPDLRITWLKSLAEFLKANSNYEEAAQVSIIAAALVNGYLKLLNRIPKELMDDFSIISPNVPNELTLPDPTLLEDVEGEICKLADFTEQGFVNLLKDAIYVLKKGSFFESCAETYRLLLPTYQRNRDWQRQRDSYQELVMLCNQITIENTVKQRIFSNYYRVAFYCQQLIPEIHGKEYVYKELNFVRLSDLSERLQKQYGDKFGDKFIHLLPNNKPVEVSSLDPAHLYIQIVSVDPYLVPEELKIRKTPFEQNTNLNKFIFEIPFTKSGKVHGESVTEQWKKKVILTTQSYFPYMKKRLLIIKKEEIELTPIEASIELIQKKVNVLRAELNSRPANTKTLQINLQGCLLLQVNAGPLAICSSFLSEKEYSKHNAEHITKLQEVMREFTNVLEFSLKFNHELTKDSEGGSELQEQLTIGYNQFREKVSEYVQLSNEVEDLSININPLALTHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3E0</accession>
    <name>A0A8J4Q3E0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Leucine-rich repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005321</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000208">
        <property type="protein sequence ID" value="KAF2073376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3E0"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052394">
        <property type="entry name" value="LRR-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="618" mass="67061" checksum="5C145188B7F42480" modified="2022-05-25" version="1">MATTKVENINELPIKTLVKIVDCLNKINSSSYYFVNYALVCKFWTQNVIPLAWKELTIATSQPSEPLIEYNEVGMLQKFGKESQSPPMGFERLVMRIGKVTEYFDALKSVTTVDLRNNPSTNSVIDRLSDSLKNNTSVTSLNLYNNRLMQKGGISIANALKKNTGLVYLDLGFNLLGANGGNAIADALKANNTLVHLDLSANQFGIRGAGPIIEALRVNKSIKYLNLQTNQLRDESSLPLADVLRVNSAFVELGLNDNEIGSKGGIALAKMLKGSKTLTKMEFGKNELGEEGGLAMADLIKFNKLVTYVRLDWNKLGVKAIKAISDALKDNTSVNYLNLSFNNFGDEGLVTLAESLKLNKAIRTLDLSRVASGLVGHKALADSLRVNKCIQTLDLTNCKIANEGGVEIARAMVDNKSISTLSLNNNLISKNTLTAFANTLNKNTTLTSLSLTNNNLTIDATENIFKALSSANKTLTTLDLSNNLLGADGGIAIAKYLPRTSFSELALANNQLTSVGATAILSSINANVHTLDLANNQIAGDVCKPLCTSISTLVKLNVSTNKLDDDIVAPLIQSIKESKSLVSIQISANQFSKDSNNSLLNTIRQNKSIFFYDLVEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3F1</accession>
    <name>A0A8J4Q3F1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Saposin B-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000648</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000013">
        <property type="protein sequence ID" value="KAF2078010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3F1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035229262" description="Saposin B-type domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="13873" checksum="39841F9F82CCAE2E" modified="2022-05-25" version="1">MNFINLIVISLFLFSTTCHGIELLFDDAKEHYSQEVINQYYSMCTILVPQYLELFKTSSLQNMSNHEIANYESQFCQNFKQLKDYELCKTLVFQKVILTLTLSSPIEKICYNIAYFPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q419</accession>
    <name>A0A8J4Q419_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000877</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000018">
        <property type="protein sequence ID" value="KAF2077832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q419"/>
    <dbReference type="OrthoDB" id="18792at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040405">
        <property type="entry name" value="DDB_G0275255-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34411">
        <property type="entry name" value="DUF6748 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035190209" description="Chitin-binding type-2 domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="275" mass="31011" checksum="4DB57671ECE1BFFD" modified="2022-05-25" version="1">MISTKSIIFILLLLVIGIYATETVTENTDAVEKVKKHHTKKTLYYTLRKDYRKCASCGGWFLKQVNSGDSKEIYVMALDYDKHTTTINDTNSDQYVVGGTLKKDQFHVSDITRCLPAIAKKDSKLRYYVYHNEKKKDYIVELNTGKKEKVSGVIEQFSTNVSHIHKEWLEAKVKTDSILLGAIGKKKFLEASSVFIRLPDPKKPCKALPLPKCTAGHVPAFSRQVDRCLKFEGCVAEGICTLSIPVCDTNYSLVSFNSKPNGCPKYYCDPSFLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q663</accession>
    <name>A0A8J4Q663_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Armadillo repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003958</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the importin alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000132">
        <property type="protein sequence ID" value="KAF2074726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q663"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2074726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="451" mass="51126" checksum="E636B3B2DCF2B493" modified="2022-05-25" version="1">MEGLPVYQDDEGKENCENLNSEDLEVVYKSLNYFVTLMEIDYYNTTDPVQKIIDVGTVPKVITLLHNSPDNLTHLACRFFIQMLCIHPEHINIIADYGVLIPLIRLVEKSSDNSMLVGIALWIIGSIAEDPKYREELYENGPLQTFLSLLQKSNASFTIGSVSLIVKNLCQGDVSVEKGFKLLEILSLVMKSNNSLSLSNGASALKEISKLKEMIFYLIEWSLVKLSLDVLKNEVYSRFYADLLLIIENTSKENPQHVFSIGTSSVILRFLFGPINPEPAPDGEDEVDPTNTTEAKRLIVLNILSNLIPSANTDQIQEFIDLHYFETLINLLNNDTDSKKVINLLFLLLKNEDVTFDQMEFLVSHFEFLQVFIIQLEAPNLSSQDKITIIKSINHILSIGDQIQLQKKAENNSFVANFTSIDGFSRLKTIYETDNTIETHYPLSNILNFEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q6V8</accession>
    <name>A0A8J4Q6V8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003425</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000106">
        <property type="protein sequence ID" value="KAF2075296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q6V8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2075296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="252" mass="29732" checksum="CC16E4F8CD9092AE" modified="2022-05-25" version="1">MVPSRLLSSFCASMSTIDEDTKVIEIVRDGVYPKDPENVILDWERKETIYLIPDESNNYTKSVWASCKEVEDVNEDDYEYYPFNRYEKGNGNPDIDIECYDKFDFYVSKDKLEQIESFCGGVGVLSETTHNLCFNLTYTYYNKDQDDFESDVLQDPELIYKLRDINKFIEYSKENINDIEFIGFNMKVKLNILREMSRLSFFCPDMVHFIGDSECKYGSEKSCNQSTCNMGRLELYEAILQIYFYKYVSRYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q7U0</accession>
    <name>A0A8J4Q7U0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002535</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000070">
        <property type="protein sequence ID" value="KAF2076181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q7U0"/>
    <dbReference type="OrthoDB" id="3784at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00761">
        <property type="entry name" value="Glyco_tranf_GTA_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049625">
        <property type="entry name" value="Glyco_transf_61_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050834">
        <property type="entry name" value="Glycosyltransf_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43685">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43685:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 2-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04577">
        <property type="entry name" value="Glyco_transf_61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="4">
        <location>
            <begin position="185"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase subfamily 4-like N-terminal" evidence="6">
        <location>
            <begin position="458"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="3">
        <location>
            <begin position="624"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 61 catalytic" evidence="5">
        <location>
            <begin position="1033"/>
            <end position="1221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2076181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1275" mass="145545" checksum="1DD37DDC0EB916DE" modified="2022-05-25" version="1">MKKLLLFLKRNFISIVIICSLLFHIIVIHKYASFNIHRDQDLVSARQSQQYLKKSNTKDQLVGGVIEKYLYKSDKYLSNYFGNGEKSINYGDNTNFEKTAHLASQHPASIVEMDSNLDTLPSLCSPIDFDQVEQCEGDKYYSPSVVTPTPVLDENKMDYTLKPNSEHRAKFAYQPQDMTQEPIITILTPFYNVGPVILETADAIFGQSLQNFQWILVNDGSPNKTLVEMYLEPFKKKSLTDPRILVVDLPKNKGLPGARNEGLVFAKGKYLIPLDPDDMIENTYLEKAVWFLETHPEYTLTNAWSLGFGYKEYYWKNGFQNGDINMKENMITVATVMRTKVLKEIGGFDANLRTGMEDWDLWMRMANNGHWGHTLQEFHFWYRVSPPGKWASIYNETKFNEFMANQKKKYPVAFTQGVPKLSRPEQEKMESVQDVIPFDNKLKKCRPRLLLLIPHMEVGGADQFNYNFAQGMVQDGWEISIATTRDAPNKWLPQFLRVTPDIFIMPRFSKVTDQTRFLAYLIKSRGFDVVFLSNSEYGYHYLSYLRANAPGPAYVDYTHSETPKWKNGGYARYSAGAEVLLDRAIFASEHLRQYCHKIGHNPNKTATVLIGIDSDKYAPKPENRELVRKELGFPDNVLVIVFVARLESEKQPEVFAEVLKRVDEMGYDFRAISIGDGLLFEDLNATIVKNGLADKVKLLGNIPNSIVSKYVSGSDVFFLPSKVEGISLAIYEAMSQGVCAVSAKVGGQAELVTPDVGYLVIPGTPTEVDEYTEIIADLASNLTHVADLGKKSREKILNGFSVKDTLFRLKEEFCNAAIVSKYTTSLFNNKVVSKLSNEVAILGFEYERASEELLPIWNTLQTTIKQCAPPAVVVGKKIERKPSPYPVHHPEEVLEHLNITTLRVVDDILEEKQKVDRKMKIILKDYQDQYIRRNPRVANKDFKANDFLHPLNADVETWNNILPYPFAQQEYLAYIPNAFIGTGASGTVFDWDRIFLLKKANKQLYTMPSDEYQRCQVIKSKKLVSLIQVYFSYGNFFTEQLPKLSLAYEEINNDPEINILVPNTPYARMTMEELLKFNSSRILYYNPGEGWDPCKVFYAEMLMLPTPIPTGSPPREMMDSMRNIIYEKNNIDIVSKEPTKYVIYSSRAMTWSKKRMVANEQEVIDTIKAHLPKNHELYIWSGDEKLTPELVHIASKARALIGMTGSNLIPMVACRSNTTFIEFMHENPWLTYWSAAESLSLVPWMLPIKGCGHDSESIQVPIEDLVNTLKLALSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q9B3</accession>
    <name>A0A8J4Q9B3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UV excision repair protein RAD23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_001470</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Involved in nucleotide excision repair.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RAD23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000035">
        <property type="protein sequence ID" value="KAF2077216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q9B3"/>
    <dbReference type="OrthoDB" id="419317at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14280">
        <property type="entry name" value="UBA1_Rad23_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14281">
        <property type="entry name" value="UBA2_Rad23_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01805">
        <property type="entry name" value="Ubl_Rad23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000002">
        <property type="entry name" value="UV excision repair protein RAD23 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000003">
        <property type="entry name" value="UV excision repair protein RAD23 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.540">
        <property type="entry name" value="XPC-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004806">
        <property type="entry name" value="Rad23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006636">
        <property type="entry name" value="STI1_HS-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015360">
        <property type="entry name" value="XPC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036353">
        <property type="entry name" value="XPC-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10621">
        <property type="entry name" value="UV EXCISION REPAIR PROTEIN RAD23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10621:SF0">
        <property type="entry name" value="UV EXCISION REPAIR PROTEIN RAD23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09280">
        <property type="entry name" value="XPC-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01839">
        <property type="entry name" value="RAD23PROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00727">
        <property type="entry name" value="STI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101238">
        <property type="entry name" value="XPC-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3 5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="7">
        <location>
            <begin position="157"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="79"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="200"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="86"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2077216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="347" mass="37575" checksum="1F0F382F7C8D3E20" modified="2022-05-25" version="1">MKITIKNMNKETYLLEVDEEATIGDLKNLISEKHNQNPTWQTLIYSGKLLNDDKRTLSSYNIVESGFIVMMIKKPREGISPAAAAPSTPTPTTTTPSNTTTTSTPSTSTTTPVPSNNTPVTPNPTPTTESTPSTSSPSTDTPTTTTTPSSTGFATGAELEATIRNIEAMGFPRDQVMRALRSTFNNAERAVEFLLSGSIPADNDEAGDEDAPVAPTGTGGQSPFDSIRSHPHFPLLREAIQRNPQIIPEILQQLSQSNPELVRQITENPQEFLRIFQGGEGGAPQTGGGQPGTFTIQVTEEENQAIERLQNLTGLDKSEVVEAYFACDKDENLTASYLFERADEPDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQP6</accession>
    <name>A0A8J4UQP6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small ribosomal subunit protein uS3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">40S ribosomal protein S3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_007716</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000427">
        <property type="protein sequence ID" value="KAF2070966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQP6"/>
    <dbReference type="OrthoDB" id="10248446at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02413">
        <property type="entry name" value="KH-II_40S_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1140.32:FF:000013">
        <property type="entry name" value="40S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.20:FF:000006">
        <property type="entry name" value="40S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="entry name" value="Ribosomal protein S3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057258">
        <property type="entry name" value="Ribosomal_uS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_uS3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005703">
        <property type="entry name" value="Ribosomal_uS3_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01008">
        <property type="entry name" value="uS3_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760:SF32">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="Ribosomal protein S3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="KH type-2" evidence="8">
        <location>
            <begin position="22"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Small ribosomal subunit protein uS3 C-terminal" evidence="7">
        <location>
            <begin position="109"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2070966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="216" mass="23847" checksum="BE7ECCEAD99A00D1" modified="2022-05-25" version="1">MNSSLQISKKRKFVADGVFHAELNELFTREFNKDEGYSGVELKTSPGLTEIIIRASKTQSVVGPNARRIQELCSLVQKRFNFKEGTVVLFAEKIMNRGLCAVAQAESLKLKLLAGLPVRKACYAIVHQIMTRNARGCEVIVSGKLRAQRAKSMKFRDGYMIKSGKPSDQLVDFACRHVLLRQGTLGVKVAIMLPSFDGGKTPGASAFCQPDIIQIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4URC9</accession>
    <name>A0A8J4URC9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Signal peptidase complex catalytic subunit SEC11</fullName>
            <ecNumber evidence="4">3.4.21.89</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Signal peptidase complex catalytic subunit sec11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_006690</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalytic component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Specifically cleaves N-terminal signal peptides that contain a hydrophobic alpha-helix (h-region) shorter than 18-20 amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.</text>
            <dbReference type="EC" id="3.4.21.89"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S26B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.89" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000319">
        <property type="protein sequence ID" value="KAF2072001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4URC9"/>
    <dbReference type="OrthoDB" id="10257561at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009003">
        <property type="term" value="F:signal peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.109.10:FF:000003">
        <property type="entry name" value="Signal peptidase complex catalytic subunit SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019758">
        <property type="entry name" value="Pept_S26A_signal_pept_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015927">
        <property type="entry name" value="Peptidase_S24_S26A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001733">
        <property type="entry name" value="Peptidase_S26B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02228">
        <property type="entry name" value="sigpep_I_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806:SF6">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00717">
        <property type="entry name" value="Peptidase_S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00728">
        <property type="entry name" value="SIGNALPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00761">
        <property type="entry name" value="SPASE_I_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="145"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S24/S26A/S26B/S26C" evidence="16">
        <location>
            <begin position="40"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2072001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="180" mass="20281" checksum="163865DCF0AD94F7" modified="2022-05-25" version="1">MSELIGKLNPFSNIPKYQIAQQIINFGLIVSTALMIWKFLMIASGSESPIVVVLSGSMKPAFERGDLLYLDMNEGPFRVGEIVVFKIEGKEIPIVHRILQIHEKSNGLVDILTKGDNNPVDDRGLYAEGQLWLNRNHIIGRARGYLPYVGMVTIMMHDFPQLKIFLVVVLALFVLSTREQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4URL5</accession>
    <name>A0A8J4URL5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">CYY_006281</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000283">
        <property type="protein sequence ID" value="KAF2072406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4URL5"/>
    <dbReference type="OrthoDB" id="9984778at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021319">
        <property type="entry name" value="DUF2921"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050731">
        <property type="entry name" value="HRD1_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763">
        <property type="entry name" value="RING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763:SF162">
        <property type="entry name" value="TRANSMEMBRANE E3 UBIQUITIN-PROTEIN LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11145">
        <property type="entry name" value="DUF2921"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="345"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="384"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="411"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="507"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="538"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="596"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="86"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="205"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="225"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="245"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF2072406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="644" mass="73544" checksum="CAD00A7A00883DC4" modified="2022-05-25" version="1">MKYLSILFIIVVYSLISNIDIVFSFDQEQLGSTASTAINQVYYPRNISHIYKGVWTETNGDLSKLSFHKNSGSILYTFTNIKYNNNNNNSSNSTTPSPTTTAANTTNSGSHSNSNNNTTTDFDIVEGYLILRDGVYSNNYQLKYQLYGIYEYKIGLLTLVALPIADNYYLATPNPLNFDVIKGMDSNHSILNLKEQMNNMSKLNQVFFPSDNNSNHNNNEDDKTSSSSSSSSSSSSTNSGDKHTSNNNNNLSSTHTSHGTSSKLTIVKSPYINDNSSSFIFVFTQQLNNLTKEQYQDLVEHPTTIHSDEHEALYFGKGQFISLFAGIVLDYQSSATRMSVYRSKILNYIVMVSLSSFVQIFILIRQMDYTGTQTGASRISIYSIGMQTLLDAYLCLLHLIAGVLIEQIFNAFATAAFFQFVTFSLFEMRYLLIILKARRPQAFADGWTSLRREFSLFYLKFYTCLFGGIMILYYFSGLLNFFVFLMYSFWVPQIISNAKRSIKKPFLWKYVIGISITRLVIPLYFYGCPSNFMQLEPVYGFSQFLVFWMIVQVVVLWLQSFLGPQFFIPKSLLPPKYQYYRPIPAVIRSREEGQGCVICMNDVEANDTDYMITPCEHLFHSRCLQQWMEYKMDCPTCRAQLPPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4URU6</accession>
    <name>A0A8J4URU6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">SNF2-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_005939</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000251">
        <property type="protein sequence ID" value="KAF2072751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17996">
        <property type="entry name" value="DEXHc_SMARCA2_SMARCA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000008">
        <property type="entry name" value="Chromatin structure-remodeling complex subunit snf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003020">
        <property type="entry name" value="SNF2-related domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014012">
        <property type="entry name" value="HSA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07529">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00573">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4 8" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="751"/>
            <end position="823"/>
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            <begin position="1175"/>
            <end position="1338"/>
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            <begin position="1479"/>
            <end position="1646"/>
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            <begin position="1961"/>
            <end position="2031"/>
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            <begin position="1"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000313" key="14">
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            <dbReference type="EMBL" id="KAF2072751.1"/>
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    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
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    <sequence length="2417" mass="273358" checksum="6BD2280F08D32171" modified="2022-05-25" version="1">MNGSPINSDFSQQQQQQQQQQYYNGGVNPATAGTTSAIASTTIGVGSNNSINSSSSSNSGNPSTPTVTTSTDPKPAYNMPLSIDQQIQKQYQLHPNQQQQQQQHFYQQQQQQQPQQQQTPPISSPNMMINKQAPQQQPQQQQQNVANNNTQNTQSIVYNNNPSSNMTNSVNSPPIPSQQQPQQQQIPKENIIQIMNYIDHLQTVKTEISDPKYQNAINWVKHIPLQQLTPMESALYSKIVTTHNNSLQQQQQIYQQKQQQQQQQQQQQQPNMNNQAFSSNNNNSNSSNNPPPQHPSMGMAKQPFYQQQPNVVQPQQAQQAQQPQIQQPQPNQVGQQQMMTNQNYFYAQQQAQPNLQQQQTQPNLQQQQPQQQQPPQQQVQYRQGNIQHQAQPQQHQSPNIPSSVPTSSMQQQFPQPLLSQEQIRMVRAQLEAYKCLIKGEPISLSCLEILGLSSLYRPEGRPPLPVGKAPVVTTPNTTPTTNVTPTGYAPTAPTGTTAIPQQPNINNNNNNNNNNFMNQQIAQQQLALRQQQGKLPIQPPSSTLQQPLQSQLTSALLPTGSSPSQISQQTQTLLNPNSAKPLSAISPTVGSQTQSALGATPNSIAPATAQLILQHQQQQLQQQKPIPPVPTIVTPPPQPLDPAKALRSDTLIPIPMDPIVIRDSYELHVKGKLTKQIDHLKELVPTLEEGSVEKIQSSIHLKELSLIELQKRVRAELLEMSDSKASTAAKSSSASRSKKSRDLAKARAAAAANDTQSEDKTKHKDFLAAIVQHGRDFKDYHALKMTKVKKMTKRVTNYFILQEKKEQQQREKEERERLRALKTNDESKYLKLLEQTKNQRLRELFDQTNEFLDKISHLLQKEKTMIEEEEENEEIERKQKEQQQQILLQQKEQQEREQQEKEQQEREQQEKEKQEQQQKVQKQLSISNLLDNSVDDDVPVDKQVEQDKDFVEQQNGKDDDKQEKDKEMKEKKDDDKDVEMKTTIIEKEVQESKVDDISMSDKVDDKEKVSSSSDSGEKIEEKDKEDGQINDKEHIDKNSKEIESKQNLSSTPQSPTTTTTSDVVKDTTDESKKEDSETKSNALVVSETQPTTTTGVPTTTTTTTTTTTTNDSNNNNNPAAPTAATAKPQGQSYVSKAHSYYSKAHSIQEDVLTQPLLLEGGTLKPYQMQGLQWMVSLYNNKLNGILADEMGLGKTIQTIALVSYLIEVKKNNGPYLIVVPLSTLPNWSQEFAKWAPKVRTISYYGDKQARKSIDISAAQFNTVITTYEYIIKDKNTLSKIKWTYLIVDEGHRMKNYTSKLSVTLGTSYHSRYRLLLTGTPLQNSLPELWALLNFLLPNIFDCVDDFEQWFNAPFASTGEKIEMNEEEQLLIIQRLHKVLRPFLLRRLKKEVESQLPDKVEKVIKCELSLFQHKMYDLIKSKGVAKLAAGNGQDGNPRLAKGLKNTYVQLRKICNHPYLFYEEEYPIDMNLIRFGGKLDLLDRLLPKLKEAGHRVLIFSQMTQLMDILEHFFVYRNFKYLRLDGSTKSEERGPLLTLFNAPNSEYFIFVLSTRAGGLGLNLQTADTVIIFDSDWNPQMDLQAQDRAHRIGQKQTVRVLRLVSAKTVEENILARANFKKELDKKIIQAGQFNNKSNRSDRKKMLEDLMQEDETAEMERQGLPTDQQINEMIARTPEEFDLYEKMDKERHERDLIEWKQEGRTGESRLCTEAELPPWIKQEVEVNDDFSFVVNPNSKRNKMEMEVDRQFMKMIENGETPRRRRTASAAISTLNSEIFEDDEYSDSSSSSSDSDTDFDDPSERAPKRRSRAQQQRDQRPPPPPVDNDSDSLSTPSHGHKRKETFDDSDDDEQQPTPNTTTTTTSTTTTTSSSSGRSAKKKIKKTPAKEKKSSSSSSSGGVGVSSSSTTTSANSSGPPTPTLPSPPPVKKQGRGRPAGTLKKKPTTENEIKYLALWELIRSDTDEEGRKRSEIFQKLPSKRDYPDYYQVIREPMDMKIIKEKIIAKQYPSPKEFARLVNVMFYNAQIYNQSGSEVFEDAVHLQNIFTNEFNKNFPNFALPKPEPPLSPSAYSQPPTSAPTTTTSTASGDETSVSNLSRISSGVSKDSDQDDQTSISKINSNIPPENIFKENHHHQSAAVVNDPPVATPKQSSINDIVNNNNNNNSNSNTYNQMNQMQINQMNHMNQFNNRPLPSINSNHNQQQPQQQQQQPPPPKPMTTPLNENKKYAFSPSPNSSPNSSPPSSPISHQRQPIPIKLLPPQKAHQQHQLQQQTKPSSNNNNNNTNNMMGNSSGFGGAQHLSPQKPKFNPMSLSSMMSNTDDEPSSPKKHTSPSPLLNHQYNNNNNNNNNNNNNNNNNNNKSSINNINNNNNDIKFSQPYNRNVANITGSKRAYSDSDESYQNSENDDDDNDNNSDDDYDDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4US02</accession>
    <name>A0A8J4US02_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_005703</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000234">
        <property type="protein sequence ID" value="KAF2072991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4US02"/>
    <dbReference type="OrthoDB" id="2160351at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051421">
        <property type="entry name" value="RNA_Proc_DNA_Dmg_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031774">
        <property type="entry name" value="SF3A3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024598">
        <property type="entry name" value="SF3a60/Prp9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021966">
        <property type="entry name" value="SF3a60_bindingd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12786:SF2">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12786">
        <property type="entry name" value="SPLICING FACTOR SF3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16837">
        <property type="entry name" value="SF3A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12108">
        <property type="entry name" value="SF3a60_bindingd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11931">
        <property type="entry name" value="SF3a60_Prp9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="246"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="318"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="325"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2072991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="483" mass="56933" checksum="19D1A616BB6B3254" modified="2022-05-25" version="1">MSSTLLEKTRNLHESIERYELLIEHEMKSTPKTTKENVLQNHRVNHYLNSSIKCANNLIEIYSDQDNTRADELSSLSRQGKLFSDFYDKLKEVKEYHRKFPNIKEDRNNVPLLFTPNVLFSGNESNGKFLDLNEIYEDYINLCTTSTKIDYITFLGSFYMFTYNDISRMKVLAYREYLDKIYKYLVSFIERTQPLLDLNQTISKCEAEFTDKWDNSDYFNKENTSNNNSNNENTELTEQEKSPLYCKACKKLFASENVFNGHLKGKKHIQSTERMNSNNNNEFKARKPTSLLEFKILKLATHLFDQIDATKENVLKKQSRKYSENEFEEDEEEDEEDITNIDDFEVPTEEPTKHRIANYPVDALSGKPLPYWVYNYLELGVEYKCEICGNRAYRGRKAYEKHFQDTRHSYGMSCIGVPNTIHFHEITKIKDALELWAKIKDQSSKSAFRSDRDEEYEDENGDVMSKKTYEMLVKQGLIKPKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVM9</accession>
    <name>A0A8J4UVM9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Actin-related protein 2/3 complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_001983</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the WD repeat ARPC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000051">
        <property type="protein sequence ID" value="KAF2076726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVM9"/>
    <dbReference type="OrthoDB" id="406844at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001331">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017383">
        <property type="entry name" value="ARPC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10709">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2/3 COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10709:SF2">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2_3 COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038093">
        <property type="entry name" value="ARP2/3_su1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="50"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="139"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR038093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2076726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="369" mass="41146" checksum="D98E1925FF642BB2" modified="2022-05-25" version="1">MSTHEIEHLATCITAHAWNADRSRVAICPNNNEVHVYAKQGTSFVCEHILSEHDQVVTCIDWAPKTNRILTSSQDRNAYVWTFKDGQWKPVLVLLRINRAATHVKWSPQENKFAVATGAKLVCICFFEEEHDWWASNHIKKHKSTVLKVDWHPNNLLLATSSSDFKVRVFDAYIRKADGRSVTRPYGEVPFGEPIFEFNECSSWVHALKWSPSGNSLAYSSHDSVFCVADFSTNPPTVEKIKLRSLPLRDLLYITENSIVGVGYDCNPLLITNSGGWKFSDEMDKASTSGAAGAEATSARKLFQNKVDLGESSKSDTKLSTAHQNCISFIVPFKSVGGVVSDFSTSGLDGNVIIWHVKALEAKNKLKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UW23</accession>
    <name>A0A8J4UW23_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_001610</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000039">
        <property type="protein sequence ID" value="KAF2077101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UW23"/>
    <dbReference type="OrthoDB" id="192247at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00121">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051165">
        <property type="entry name" value="Multifunctional_ANK_Repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123:SF33">
        <property type="entry name" value="PROTEIN HOS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00061">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="205"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="MATH" evidence="6">
        <location>
            <begin position="252"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="MATH" evidence="6">
        <location>
            <begin position="392"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="538"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="605"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="638"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="671"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="814"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="884"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="994"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="54"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="739"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="62"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="741"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="754"/>
            <end position="792"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2077101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1161" mass="131102" checksum="A60B79FDDCE7D3CE" modified="2022-05-25" version="1">MSNVVSTGSSSSSSSSSSSSSSSSTSPTTTTTTTTTTTTTASVNLNIVVPNNTYLSNNYHDSSVNNNNNNNNNNNSGYSSNSGYNSNGNKNNINNSNVIISNSSSSSSNGNNNSSNNKTTTTTTTTNTSSTSKIIDAARKGNVEIINKLLKKNWKKINKRDERGDTCLHKAASFGHLECIESILKYSSSNHLLPNQVNINNRNNEGYTPFMLALLNGWKECAKVLLSYGSDLNEKLPNNPGIPIAYSINNGTGVYSWKIENFSRIKDRKIYSNVFLVSGFSWKLVGYPRGSKSDDNFSLYLEVANHEALTEGWSHLANFTFTISNQLDPTKKVTREVMAHRFHKNHTDLGFSQILKKDMIKDKKAGWLVNDTLYVEFKIEVLQNFIYNVDESPSYTWKIQNILSMKDRATSPLFRVGNCNWMIALYPKGKGGGNNLSVYLKVADGNSLPQDWFYLVSFKFSIIDQKTGQKFTRQVEGKKFKANVEDWGFPQFMKLSLLYDPTSNGFLKTEDDSILIELQMEIINDFTRKKPKENLNYWGYSLLHWFAYKGSYDYVRFLLANGANPNIIDSKGRTPLDWCAYNGDLQTTELLISIGGADLNSKDHEGFTPLHKAVMNGHLEVAHLLYKFGACVNAKHTSRVSPLELAIKVNRPWCVESLIEWGADLNLSDRNGRSPLHWAIFLGDPKLLSLLLNYGCPDTIIEKKSINNNNNSLMVTNSSSQRNSYEYENLIDTQIDDQDNQFEGMNNDDISYSNNNNNNNNNNNNNNNNNNNNNNNNNNNNNQIINNQNTNINNINNNNMKSKTPININCKDQDKYTPLHRAIWRGKRSFVKFLLEKGADPTLCTKDNRNSLHIAVAANEYNIAEILLDYTDDPDIILHGFDQLNHTPLHKAVINGNLKMMELLISRGANANRYQTTPAINEAIKNNDILSMVYLLDLDADIHKIAKPLMHLLSKKVKSFKSANELAGGAIENDISPCTLSKDLMYLVNNEQYKDITFIVEDKPIYAWKGILCSRSDYFRAMFETPLKESLESQVKMESISYTSFLHIMEFLYTGGIPDESCLSFEETIQLFVASNRFMLKRLKLLCEKRMIKNLDVHNIYTMVNLTDIYESTFLVDECVKFLSDNYFQVPQTSQILKIPSFRKSLLDVLKKCSISSSNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWB6</accession>
    <name>A0A8J4UWB6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Blue (type 1) copper domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001314</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000030">
        <property type="protein sequence ID" value="KAF2077386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWB6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035327934" description="Blue (type 1) copper domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="176"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="219"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="28098" checksum="E546A17F265781C3" modified="2022-05-25" version="1">MRFAIALIIALALFANAAWARNHYINWEDHKHEDLTLHVGSTVTWISGDEAPHRIQSVDKMELKGLDFNSAKNATHTFSNEGEFLYSCAFHPEHKNVIKVISLVPQKAVYPWLAGEKKADPYSAAALAQRNKRIIYEKTVNSKKNANNEELVPKNSTATPEHPTPSVTPSTKGTHPATPSSDAPTKPTKKPKKKDSDETILPKPGDSSAHKGKGGINDEAAASTEATKAPASSQSFATKTSSNIVLLFVSCLFVVLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWW2</accession>
    <name>A0A8J4UWW2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_008512</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000531">
        <property type="protein sequence ID" value="KAF2070170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWW2"/>
    <dbReference type="OrthoDB" id="449052at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02205">
        <property type="entry name" value="CBS_pair_SF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF153">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="109"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="180"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="259"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2070170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="312" mass="35598" checksum="0E38153254CB9369" modified="2022-05-25" version="1">MSNINLDVLYSIKVGQILANDKTGEIYSVHSDYPVKDAFELMIRKKVLSLPIFDSVHRKYNNFIDMVDIVCFCMKHFSKKELIDFDMNYILESKQIFEKFKCGDICDLSGRNAYLPVESTAPLKVAIDLMSKWGVHRIPIIDAEGTLISILTQSKIIEYLYKNLEKIGDLSVLLQDIPNMGNPNVITIQKDALVMDAFKLIQENNISAIGVVNQIGILEGNISVSDMKMVGYDGKLLSRMFLPVETFMDMIPKNPTIQMFNNILCVRDTTTLEETITKFYLSKVHRIYKIDHEGRPKSVISQGDVLKYFSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWW6</accession>
    <name>A0A8J4UWW6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_008490</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the proteasome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000528">
        <property type="protein sequence ID" value="KAF2070185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWW6"/>
    <dbReference type="OrthoDB" id="37597at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03761">
        <property type="entry name" value="proteasome_beta_type_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000156">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF3">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6 9" id="KW-0647">Proteasome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0888">Threonine protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2070185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="270" mass="30029" checksum="16F2EA046F928464" modified="2022-05-25" version="1">MNIDFEALKEPTLFDDIVDDLKFLDKPHFELPPNSNPGEFLQHVFEHPSAPKKLEFAHGTTTLAFVYAGGVVVAVDSKSTQGPYVASRSVKKVLEITPTLLGTMAGGAADCSFWERELGKRCRLYELRNKELISVAAASKILANIVYSYKGYGLSMGTMITGWDKTGPCLYYVDNDGTRLKGQRFSVGSGSTYAYGVLDSGYKWDLNDEDAYELGRRAVYHATHRDAFSGGAINVYHVQQSGWKKISTDDCFKLYQKYYDQVPINKPAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UX79</accession>
    <name>A0A8J4UX79_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MRH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_008106</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000473">
        <property type="protein sequence ID" value="KAF2070575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UX79"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23361:SF20">
        <property type="entry name" value="MRH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23361">
        <property type="entry name" value="MUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="4">
        <location>
            <begin position="96"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2070575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="237" mass="27210" checksum="C0AF82AB3C547DDF" modified="2022-05-25" version="1">MKLLHYTKLHPTIAFLGSSESCERYFGYQRLKRVLMPRVLMSVDIFRKYILPICKYQVKRDFQQINTGKMKVLALVFFITLSSFFIATSAKTTPLESCTFDTEHHQYNLAPLGGLSFNASVGEPGERFNIVFSVCGYNYRCLKYKELSGSEKKYQSCQLYEDKYVYQSGLPDRYTVTDNGNGVDITYSSDTFYSACIRRNKLSFTCDMHNEFQITSTSTPVACLFEIKISSRHACPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXT1</accession>
    <name>A0A8J4UXT1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007829</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000439">
        <property type="protein sequence ID" value="KAF2070858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXT1"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47932">
        <property type="entry name" value="ATPASE EXPRESSION PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47932:SF74">
        <property type="entry name" value="BOX HELICASE FAMILY PROTEIN, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="274"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1218" mass="142292" checksum="9AE259689BFEF2D5" modified="2022-05-25" version="1">MIPYLHHIRKIRSTNNGNSVIGPSIKNHHLLGLNNNYSSLLSSSQVSLHRQYASDNNKHNEDYTKNKQMKRDKDRRQGHWLKALTNLFSFDGNNNQQQQQETHDSNNISNVVDQDKFIQTIKNQAIEKINKDKSKTIEEEIKLEETIITPTTKTNSTNEQEDVEIKITSEEGEDVTLVLDEQQKQQKDQQSTTSTTIKEPFTEIKDPEAFLEEKLKQAHDQLIKQKNIQSSFKPIEKEKIINELKQEEQQEKEGEGEEISIVELNLIEQQQKQSKFEQKRKTPGTEEDDDDGLITNGAFDLSIPIESLSFSQKSWRKKFRQILLDAYNPRYIENYLGEATKQYNQKYDDDKLLFSTKTKQLDIFYEPGTIKALFDQMNWEEIDVQSYICNAFISYFTLIKDNDYINRSIELFKSKGFAYTPMTFSTMLDYYSQQGDKETVSALIETIKSKYSLKMPDSLMVPLMKACLMADKFADANMFYTLILDSTYRYNTQVLDHLLQYHLAKSNSSTILRFFQRNLPYSHDYVKNSKYFGTFMTEAIKKGIASQDIISFLISRKLVNPKLDFFNLYFHLLRHSDPTMRRYGAQLYNTFSHKKPNMKGQEIYSNSLKALMLIGDADNVMDLFHHYANEKTLFFHYQAMLEFFYNKKKHQHLEKILDHMCENKFYKMAFIPLLYFELTGNKELVQIIEKRLLVRGQEPQCAELIPTVTNYMIYAYLSLDMYEMALTWLGKKSIDYKSSIPNSLYENFATYHKERNQKGLEQIWLSHLVEKKINVIQSTILREDRMEDKPAKEEHQQKEKNLLVTTLLSELEHKWAIINVYNPTTETLKSVVDKYNANKHQNPLFEENSKSKRFIPELVQTLQDQYLKKDIIPPGASLIKAFNLLAKHAPTRYLEMLEKASPRIRAIVFNPTQLSLTFDSNFGEALSFLVQGKDLSISSITFNTIAHHLLKRGQVELAICVIRRMMAEKMRIDVDSLLIDILVDIDKVNHPIVQELTNYFSYYNYIIPRIVTSELDRLIAQGSVDEAYEFFMKAPKYPETLKAALGIFSAKFPIPPITNLMHWSSLVQLSTNTNRLFYEYFYETLYNNNLTNIVNLYVDKMDDDSFYFMSQKEFLIVLKSCKNAHKAIKLLKKYPHAELLPQTIEEAKRYNAIVKDVEISYRLEYKTKIYTEPAPEPLYTFKQIEQPDVETILSNFTNINPTDVIIETGPTGCRIISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXX3</accession>
    <name>A0A8J4UXX3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cation-transporting ATPase</fullName>
            <ecNumber evidence="12">7.2.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_007622</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.-" evidence="12"/>
    <dbReference type="EMBL" id="AJWJ01000417">
        <property type="protein sequence ID" value="KAF2071068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXX3"/>
    <dbReference type="OrthoDB" id="48943at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140358">
        <property type="term" value="F:P-type transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000023">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000068">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047819">
        <property type="entry name" value="P5A-ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01657">
        <property type="entry name" value="P-ATPase-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF14">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12409">
        <property type="entry name" value="P5-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="514"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="554"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1020"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1048"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1088"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1129"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1164"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1202"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="domain" description="P5B-type ATPase N-terminal" evidence="15">
        <location>
            <begin position="186"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="14">
        <location>
            <begin position="366"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="39"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="96"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF12409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2071068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1261" mass="142374" checksum="31CD5A0348F4D2BA" modified="2022-05-25" version="1">MIQNENHDEYSDVDDANENNNNNSGSLFGLKEKLQKGFSSIASSSVGSSSSPSLEPRKTKKRTIHKESPPLLSTNNSEDENEDDNDVLQDIDINDGSSNNGGFKLNNNNTCSLSSGEDEGEASPPLLQCNGGESISLQQQQKQITLSKQSSPIMYGKINNDFKKKLEKLENNDQQEEDDMLSIDKMVGYAYCTNGEVLYWISIIFTAGIAFLIYYWFNIFYLNLRYKKVSFSSSNLVLVHSCDKRIETYATKSFEPRKLNEIYSKSKLIQLVDQGLDYQEHAQQHLFYGDNEINFPIKSIPLLLLEEVLHPFFIFQLYSVFLWMAEEYYYYAIAIFLIATISATLNLVEIRSNLLSLKKISYFSCMVDVLRDGQVQELESKDLVPGDIIQLRQNFTMPCDVILLTGQAILNESMLTGESIPVTKYSILSDADIQAYRNVSVDGFRDDTKDFTREKRSLLYGGTMIMKLTGGTGSGTDKKVYGMVRHTSFQTTKGKLILSILYPKKSHFKFFAESLKFVGVLCSIALVGFSISVWRLNSLGEDPKTIVLRALDLITIVIPPALPMAMTVGTGFAMVRLKKSKIFCISPPRINMAGKIQVFCFDKTGTLTEEGLDLYGILPTIYQQQQDTQAGSEVAFTQVCKDITQFDNYLFKMLMASCHSLSVIDDKVSGDPLEVKIFEATHSVIHEDHASHYISIPHADPMRPNENITYLEKFDFQSQFQRMSVLVQFQSNKQSYSFVKGSPEMLKKLSLSDSIPSDYDKQLAIFTEKGYRVLSCAYKPWDGPITADKDELRLKTESQLIFLGFIVMENKIKSQSPPTIDILQKANIKTIMCTGDNPLTAKSVAKQTGIIKKDSILFMGILDTCENADDGSVITWEHISKEKGDYHRLDPSTLTLDSDCSNDFNLIITGPVFKKLYHNYLATGSQKFNNMLKRGIVYSRMTPDEKQVLVEELQRVGLYVAMCGDGANDCGALKAAHIGISLSESEASIAAPFTSTVTDISCCPTLIKEGRASLAVSFKLFQFMGMYSLIQFTTVIFLYFIASVLGNWMYLYQDLWIIFPLVIFMGMTRPCDTLSIKRPSGRLISGAIVGSLVVHVLVCISFQTVIFFFLRTKSWYNDDIIDDENIINYVTTTLFTYGCFQYLIIAFTLSFGKPFLKPLYTNRYLFLTYLITLITSLLILFLPTQKVWDFGQLLVLPVSWRLTLFGFICANLVLSTIVEFSFYMYKKRSKKKKVQNVSLIYSKEVPIDPRITENSVLLPIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYB8</accession>
    <name>A0A8J4UYB8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aminopeptidase</fullName>
            <ecNumber evidence="11">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_006979</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9 11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the peptidase M1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="11"/>
    <dbReference type="EMBL" id="AJWJ01000348">
        <property type="protein sequence ID" value="KAF2071698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYB8"/>
    <dbReference type="OrthoDB" id="15827at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000001">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000002">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000002">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF174">
        <property type="entry name" value="PUROMYCIN-SENSITIVE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3 11" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="14">
        <location>
            <begin position="21"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="12">
        <location>
            <begin position="235"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="13">
        <location>
            <begin position="525"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="307"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="10">
        <location>
            <position position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2071698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="858" mass="97250" checksum="B075EBD74F956E24" modified="2022-05-25" version="1">MCDHKHLVDKNDRLVLPNNVVPNTYDLHITPDLKNFTFKGEVDITVEVTKPSKTITIHSIELVIHTAKLGDQVASKIEYYVPEEVAILTFEKEIEPVKSAKLSLTFDGILNDQLKGFYRSKYVVDGEDRYLGTTQFEATDARRAFPCFDEPALKAVYNVKLTVQSHLTALSNMDVTETVENADNTKTVSFATTPIMSTYLVAFIVGELSYVEAYTPEKIRVRIYKTISQTESSEFALDVATKALSLFINYFGIPFPLSKCDHIAIPDFSFGAMENWGLITYRESILLTSEKTTLLTKQRIANVIGHELAHQWFGNLVTMEWWSQLWLNEGFATFMGYFVTDKIFPEWNVWLDFSDTYRSGALSLDSLANSHPIEVPVRSSSQINEIFDAISYNKGSCVIQMIEGRLGDNFRKGLNHYLNKHSYKNTNTEDLWESLSLLSGIDIKTFIDSFTKYSGYPVIDFKTTQTPGTFELTQKQFRLDGSAEQPTDPIWNNYIKVETASGSSEFILDKKSDTLTVPNFKQGDWIKPNFGQTGYYRINYDPAVLATLIPRIKSLELPSVDRLNLITDMYNLCKTGSVSISSYMDLIVSYSNETESPIWNTICDNLLALATFVHDQPYYPKFASIVSKLLKPISKKLGFDPIKGERSSDTLLRDKILATIGEFGDQEVIQESKKRFELFKNDPSSLPVDIRSAVLANVIKNGGETEQQDIIDRYLKTTAASERTSFLAAIAKSPKQELVEKALDFSLSSNVRSQDTYIVWVMTPSALKPVVWQYLIKNFKSIYAKFPDSGLFPRMISGSLQPKMEESRIAEVEKFFTENPVPAAARSIKQDIERIRINSKWANSFIPTLGEWVEKHSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZ98</accession>
    <name>A0A8J4UZ98_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000153">
        <property type="protein sequence ID" value="KAF2074310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZ98"/>
    <dbReference type="OrthoDB" id="23602at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1101"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="223"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1116"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1167"/>
            <end position="1190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1230" mass="142546" checksum="49FD4C6AD23EC2B9" modified="2022-05-25" version="1">MNQLFHSKSYKATTLLCKGGNFINGTNGRLYSNKNSALSAPEIEEFFVNLKSTRKALDIDPLPVNLEALEADKEEADKQIEQPRPLSYYDPYRQTLSKKFRERNDAVMAPGISAGKKDFKSLPHQRLYNDKQSKQFMKQQSPSQLEQRLELPIEDEMKYKAKQIKYIHRTIREDATKASKFRIQKTEMQPISLYPSQLWIKETGVSKASGNIGGDDNNDNIENDNNNDNNDYGDNKSHVAPNEFLVNRTKALIVDNLFPLDFNSSNFYQTNSVVNEAKRDINDKSFNDIVDDITNESVDEINSNDAPSVLKYQNKKTKDPLLVHQLKLNYGLDCERTHSVFESDLKLGKYTSTEVNELYQWIKNTKHFLQPSVINTFLQYYANQQDEKVFGSILNRYESNEIEFDSISFSIILTYFCQKQETVSLKYWIKTIKTSQFKITQSVLGPDLVRAYLTLDDLNSALDVVKLFRETNSSMLNNTCYRSLHHFVNYLTPRKEYDKLVEIIRVYAVHSPNFILNSIFYKLLIELWRNDYNLSHLVTELEESGLCSQNFYRNCVAYLLDMDQYDLSQTIYEIYLQRHKPNFRMYNLFMVYMIKNDDYSSCLDLMKQMDSNQIASDAIIDYSILEMLARKNQLELLDTFIDYLFNSPSCWKKMSFIIVYCQIHSIDTIFSRVVAKIPNLPKASMSILTNQIIRDYLKLKRYHTALRWYGDRIVKYNLSPNKFTLDFFVFYHESQIKELKAKRAHTQAIASEKTLLEFWKSRHQAFKIHKLNYPNKHLQDAYDNLEVMGVMREIDKLTPMGLLRTQVLRSIPNFLTTTPSSQQMELLHKNIIEQSMLLQGTNEKKDFHLTHYYRLNQTDNLQRALLKYIESDTLPYGPIFLQAMIDIRTQDPSAYVSLLKDCPANMRSIVFDLKFYCDFIKRDISNAMTLLAQENPLLWKDSDVIWNSVVVGLLTAGQLDLASKSIFSATTTLRILDITEVQERIDNPGPEIPISQESFDFINANFINAPEPYIWKRKKKEGKERRANAAATATANENAAASFEHDIESITNNNVNNSNNNKNETINEEFEEQSHIMTMNTSLTGKPKVFMTNIELSEEDIVLDRPDSSRKEKKNSNNNTTRFNSSSSDDGAQYFKINQIQDDYDDEEGTHNYDLDTYDPSSISQAKPYKSKVSSSSFKTSSSSSSSRPKYSGKKSYKNTTNATKETIDYSAIFRTNKYNFLDPDSVNQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZQ2</accession>
    <name>A0A8J4UZQ2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">3-hydroxyanthranilate 3,4-dioxygenase</fullName>
            <ecNumber evidence="8">1.13.11.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">3-hydroxyanthranilate oxygenase</fullName>
            <shortName evidence="8">3-HAO</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">3-hydroxyanthranilic acid dioxygenase</fullName>
            <shortName evidence="8">HAD</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_003962</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 8">Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>3-hydroxyanthranilate + O2 = (2Z,4Z)-2-amino-3-carboxymuconate 6-semialdehyde</text>
            <dbReference type="Rhea" id="RHEA:17953"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:36559"/>
            <dbReference type="ChEBI" id="CHEBI:77612"/>
            <dbReference type="EC" id="1.13.11.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the 3-HAO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.6" evidence="8"/>
    <dbReference type="EMBL" id="AJWJ01000132">
        <property type="protein sequence ID" value="KAF2074730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZQ2"/>
    <dbReference type="OrthoDB" id="204928at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00330"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000334">
        <property type="term" value="F:3-hydroxyanthranilate 3,4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043420">
        <property type="term" value="P:anthranilate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006569">
        <property type="term" value="P:L-tryptophan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019805">
        <property type="term" value="P:quinolinate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06123">
        <property type="entry name" value="cupin_HAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00825">
        <property type="entry name" value="3_HAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010329">
        <property type="entry name" value="3hydroanth_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03037">
        <property type="entry name" value="anthran_nbaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009763">
        <property type="entry name" value="PRK13264.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15497">
        <property type="entry name" value="3-HYDROXYANTHRANILATE 3,4-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15497:SF1">
        <property type="entry name" value="3-HYDROXYANTHRANILATE 3,4-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06052">
        <property type="entry name" value="3-HAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 8" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2074730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="181" mass="21104" checksum="DEA9303C6AA67EC4" modified="2022-05-25" version="1">MLLPPINISKWIEENKHLLSPPVGAALLYTNPDSSYVIMIVGGPNQRTDYHINQTDEFFYQFKGDMILKVHYQGEFKDIKIKEGESFVLPGNTPHSPQRYKDTIGLVLERKRESQSIDRLRWYCDNCKEILYEESFHVDNLDLGKMLKPVIEKFYASEELRTCRSCKHISQKPVVNDNLPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V009</accession>
    <name>A0A8J4V009_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Alpha-type protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_003724</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000120">
        <property type="protein sequence ID" value="KAF2074985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V009"/>
    <dbReference type="OrthoDB" id="301415at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005826">
        <property type="term" value="C:actomyosin contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016905">
        <property type="term" value="F:myosin heavy chain kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031037">
        <property type="term" value="P:myosin II filament disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903013">
        <property type="term" value="P:response to differentiation-inducing factor 1"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16968">
        <property type="entry name" value="Alpha_kinase_MHCK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.200.10:FF:000002">
        <property type="entry name" value="Eukaryotic elongation factor 2 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:003342">
        <property type="entry name" value="Myosin heavy chain kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001299">
        <property type="entry name" value="Myosin heavy chain kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.200.10">
        <property type="entry name" value="MHCK/EF2 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004166">
        <property type="entry name" value="a-kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051852">
        <property type="entry name" value="Alpha-type_PK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45992">
        <property type="entry name" value="EUKARYOTIC ELONGATION FACTOR 2 KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45992:SF8">
        <property type="entry name" value="MYOSIN HEAVY CHAIN KINASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02816">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00811">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51158">
        <property type="entry name" value="ALPHA_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Alpha-type protein kinase" evidence="12">
        <location>
            <begin position="563"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="947"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="1028"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="1068"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="1109"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="499"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="370"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="510"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2074985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1143" mass="129173" checksum="42F611031E563514" modified="2022-05-25" version="1">MFNISKKKKDSTPGTPISSSQSTSSGSLMSPMVTPASPGFVSRQCPFKKFGCSSFLVSKAEYENHLKEDSQFHLQLVVEKFDHQFDMHTQLMAHFTEQMEDQLEKTMKVVRNHMDTFGSTIQGKIDEGVEKCMTFSKKVEQQQQQLAKRLITQQIVEKKSSSPMVKGMDDLSIASNQNDGANQNVKAMLETELNGMEIRFKKDIESIQQEFSNKLDKKINEIDYKVGEKIDKKSMTNAVDSMLEKSSSTLELLVLKKVEEREKKIQLNIEGKLESIKDKIKIVETQQLDSQSEIRKLKLLDSGSSNNNSNNIPGSRSSISYGLSSSSLSSFTPSSNNNLNKSEVLEEIKKLEEKIYKKISEESGAHKSDLKDLSSSVKSQKNDIESLEKDCKHQFFKQSDQIKQLEEDLKKSDSLLLLMQNNLKKYNEFVDREKDRESERIKLLDSIKKLEANQKKIEAEIQEGNEQVERVLREEISSPINSVSKSPLLSKRNSLLNNNSLLNNKDKDVNSSSSLNNSTNNSTNNSINNSPSITSTSPQNSNICSSPKSLESNMDGETGILWEYDPIINKWIRLSIKLKVERKPFAEGALREAYHTVSLGVGGDENYPLSKNHTFPSIDSLSPISRKNEATNQLKNGTKFVLKLYKKEAEQQTSRELYFEDVKMQMVCRDWGNKFNQKKPPKKIEFLMSWVVELIDRPNSNGTPLLCSIEPLLVGEFKKNNSNYGAVLNNRSTPQAFSHFTYENSGKQIIIVDIQGVDDLYTDPQVHTNDGKGFGLGNLGKTGINKFITTHKCNAVCALLDLDVKLGGVSSLSNKKQLQTGTMIMPDIVEELYATDNNIKIGAKELPKAEFTRKDLKCTNTIQSFRERVNSISFFDQQRYLCAGYGDGTFRVFDIKDNLKCVHTVSGHRKSIECITTNSQYIFTSSPDHTIKVHAIRSSGFKTIETLVGHTGEVTCIVANETHLYSCSYDKTIKVWDLSTYKEVKSFEGVHTKYIKSLCLSGRYLFSGGNDLIIYVWDTETLSCLFNMQGHEDWVLSISSCGPYLFSTSKDNVIKIWDLNDFHCIETLKGHWNSVCSSVVKDRYLYSGAEDNSIKVWDLDTLECVYTIPKAHSLGVKSLLMYENQLISSAFDGQIKVWDWTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V091</accession>
    <name>A0A8J4V091_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003359</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000104">
        <property type="protein sequence ID" value="KAF2075330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V091"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2075330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="121" mass="12697" checksum="FB2A3A259F93B393" modified="2022-05-25" version="1">MLPTSGAPGDHPLSNSLIYLQLVFAFGTDPPSCSALSKGNLGGILDGRLVSISNVPLFNSTESNAVIGRGHQDSSNLRKAVIDPDFSVLLAKEQQGSICTTDNITNSKKLTTAKLVSNIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0N8</accession>
    <name>A0A8J4V0N8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009204</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000654">
        <property type="protein sequence ID" value="KAF2069482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0N8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="97" mass="11626" checksum="8C5F5788B79E7EDF" modified="2022-05-25" version="1">MIQITMKGLILYSNMFLYKLPNLQESLVLLDRYKNCTIELVIECYYRYFKKFLEPQKLLVLTNPNSCESIQSEMKSLLDVDLNIVDFQDIWIDYNKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0V4</accession>
    <name>A0A8J4V0V4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal RNA methyltransferase FtsJ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000123">
        <property type="protein sequence ID" value="KAF2074908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0V4"/>
    <dbReference type="OrthoDB" id="20105at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37524">
        <property type="entry name" value="RIBOSOMAL RNA LARGE SUBUNIT METHYLTRANSFERASE M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37524:SF1">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE FTSJ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="1">
        <location>
            <begin position="333"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="502" mass="57803" checksum="C0EF63E6E96076E3" modified="2022-05-25" version="1">MISINRFISPPKAIRLLCSRYYCNTTTTTTATATKPNISSDNIFTNEKQYDGYRTLTIVKGKESNHESFLLQQLRDNVPNCGWNKSKSPFIIVGNLSPWTIKQFQLKKHGITNKDGDDNNSNNSNQDHKPFCGFAQQILPNSILVENKSLKQIVEYASEHIIASVKNCNYKKWRIHILSPQGYIRTRQGKLYSIEQDIVDKVSKTHPSLSKLFNHQATKNQKEIIPFEDQETLVQILFTSNHSKKASAYISVSTPPIIDYLSNIQNHIPAGQIIEFTSHVKENLLKLFVGDSDNKRNIHIDHIISSKQMDPLSITQEIEDSVQDHLIEKFIPSRSYLKLLEMEIRYPQFFKMLPNQLVADLGSSPGGWTLYSLSKGCKVVSIDKSPLDAKYFDFSDKKLHFLEQDAFEFNEPPHTIPFDWLIIDMKVPPKKTLELLEIWLKEKRCKHFVVNLKYLKSDFIQETNRFIQANIMPNCTFINAHCLDTSGDYELTLFGTNKHEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V125</accession>
    <name>A0A8J4V125_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008677</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000558">
        <property type="protein sequence ID" value="KAF2070007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V125"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32556:SF18">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32556">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="329"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="329"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="546" mass="63617" checksum="B9DADC4713A9DE83" modified="2022-05-25" version="1">MLSTYLIKLILSHILAESKRDMIDFYGSRCSEFVYVWGSNFALVNKQWFLIAKELMISHSNVLINFLNHDDEGNDDNDNDDNSNCGDDDCHDANIDDNKNNDNKSSIKRSLCGGQQLQQRHKKQKISNFKILSKKSVQHINVRLAEIDDYFEKINQCTSLRSITVVHHHGNEDGDYYSVALSYHKLEVLNKLKRIDKINVNFFLNIGSYSEPSYQTEIEPITFKFKTLHFSVYHDNDTGNPFNFIYGLINLLRPNSVDFYSIDETDEGQSHLTFFHSLSKLSFESVVIDQDYISLYSLYRFLGSARLTVFKFNLQFGFLSALYNNNNNNNNNNNNNNNNNNNNNNESKYDFNRMDTTDGQKINEDNQIYCVYYGPSSESIPGYSRSLWKECLQLLKTNTILTELSVGNTRCGYDNCFGTETLNQQLIDDLLDSLSNNKSIKTLYFDFQDLDEYHTQKKIIIDKNLRQRRQKQPKKRHPFINADFISSLLQNNTTIERLYIGRERNRQWEKPLLDEIMNSKPPSTKCFIGYAPNMIMSYQFSSKAYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V157</accession>
    <name>A0A8J4V157_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Formin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_001982</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the formin homology family. Diaphanous subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000051">
        <property type="protein sequence ID" value="KAF2076725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1668162at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010472">
        <property type="entry name" value="FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014768">
        <property type="entry name" value="GBD/FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46345:SF8">
        <property type="entry name" value="FORMIN 3, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46345">
        <property type="entry name" value="INVERTED FORMIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06367">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01139">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51232">
        <property type="entry name" value="GBD_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBD/FH3" evidence="5">
        <location>
            <begin position="375"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="domain" description="FH2" evidence="6">
        <location>
            <begin position="861"/>
            <end position="1248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="114"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="258"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="390"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="773"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1256"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1367"/>
            <end position="1592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1669"/>
            <end position="1948"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="63"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="115"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="259"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="390"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="401"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="419"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="782"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="811"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1256"/>
            <end position="1266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1303"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1381"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1414"/>
            <end position="1439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1495"/>
            <end position="1506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1509"/>
            <end position="1529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1540"/>
            <end position="1559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1575"/>
            <end position="1585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1684"/>
            <end position="1695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1699"/>
            <end position="1717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1727"/>
            <end position="1741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1770"/>
            <end position="1789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1793"/>
            <end position="1802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1837"/>
            <end position="1853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1874"/>
            <end position="1908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1909"/>
            <end position="1918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1919"/>
            <end position="1948"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2076725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1985" mass="219118" checksum="DAD66772E356E678" modified="2022-05-25" version="1">MDDTISSTQGEPTSLPIARENPVTTDNSITINKDISGGDDDIKKSSISTSTPHNNIIEKERTSPLPSSPTTTTENNSPATVPPELSKQQSFVKGFVDNFNKSKLSPIQPKIITKGGASLQSSPSSGNLNSLKNSSNSSSNNNGSLRGSSIHNSSSSLNNNTPPRSATSLRSSHEMEFPGINHTVKVSAWGLLRLKDGGHVKDFELNQIETSFGRGNGEHGEQINKGNSLVMPDYGFSLDKWISSKHFSIILKKTSMACSPSSPMPGSDSPLSTNSFNESESSQTSGVSSSSSKSMSSKESHHFIVPYIVDNSINGTFIKKQRLKKGERVLLEDNSEITLSFSESLGTTNPANSKSITFLFKNNTTLMSEEDREALLDLIPEDIEQPLFSTSASPFTNSPINNNPQFNTSTNSPNPTNSKIQRGSSLSNLNQSKNRPDALYAQLKADHSLKNIQYLNQYLKNCDEHLRESLFQTDTAALLIEIISNNNKKLKLTDMDQQIDGEIVDILHHTCKFSNGVKSILKCNEAMFSDFLFLILNHNQYNANLKKQVLSICISLTSGNEQGHRLVMSAIEKIQSIKRDRQRFKWIVESLVLEHDMDYKVQCMKLFNCLIGGTKSSQAKTRIKNEFISVGALSRIQNILHQDSCPELDSQIAKFNSYFSMDEKESNCRTINLKDPVSLLIAIQQQLRTEKNDASALTSHLHDLFTISFLDEGLASSSGNPPQGVNRTNALALLSQFSKTLSSSVHSYDDFKKSIPNFEENLRKYAGIKESTSLHASPLAKRQIEKKEDNEKKEEEKKEESAPAANLKSPPLVPPPMLPVGPPPPPPPPPPNGSKCGPPPPPPPPGAPPPPPGAPPSASKKSKADMPSVQMKQLYWAKLNHTKIKKTIWDDKFDIKIELDKPSIENLFCVKKVNAVAKKETEAEKKVPSLLEDKRSYNIGILLTKFKFTPIWIIDCLNSVDEKKLTKDTVAVLQQIVPTAEEEEKLKNYDGDKSQLPFVDQFLIEILKVSKIRQRLECIMYKITFDSNLDEVITSAKAVESASEGLMKSMQIKLFLHYVLKIGNFMNAGSAKFADASGFKIGFLKKVGDTKTIDNKSSLLRHIVSIINEKNPSLLITPTSLPSIEKASRLLWTEVLELANSLKKGMASVHREVDLQLKSTGNDNFTTKFKHFVSTKESHIDSLQVFIKQVEECYQNALKYFAEDGLQPEEFFTMIFDFINQVAKAHKENLEEIAAKLPKEKKYATKNGAVLNTISTTNFESTNTPSDEIDTRSPVVAPKEEKEKGGSFFSKFRGKKKNKNGEISSTESSPAIGPISSSVDSTPIGQSPKIKSHKKKESFLSHFSPKLASLSPKIASLSPKIKSKFNSITRSLTKKHSSKNSQGESPSTSTQTPETLNNGNTKVNEKQSMEPLQEISQPIPVNNNTKPKIGSLKNNSYLHNTKKSDDPTPSSSTSSKKDLPTFVVKKNPLKHSRSFSYTPQSKISEKRGHMRSKSLSRVNKLQSNPFMQFEKKSIPPKDKVSTLKKDEKLQQPSAPAVQVEKPKPDPPHPQVKESIKKNFPDTVPPPAVKKEDAPTSPTQPQQLAESPSDFREVKLKTTGKSLIDEEKGIDKSVSPGKTRYQQIHKTFNGRKINNLKKPHQRTLSLNAKDAELIKKGNINIDDIKEKLKSQQESKPYNKHKKRNSITFADQQSNLKSNEKLSTLIEESSSSTSSVKSTPTMKPVVHQDFNQQEPANQFNLRPTPSKEDVKPISNNNLKAHTTLKPTKFATPPSQSNSITSSPLESASATATPPLKEDSLKKEGFFTFGKHKKSKSSSSEKKKENPLSQSSESLPIPGDSPSAHSDHSTSSVPTPKSKKTISKFFTKLFSKKSKSSNDNGSGSSQESNSGGDHSSPQQQQQGSLGNSPQQAFQEYANNAYNTASPTNSITSITTPTSTTSASSKSTPSKTNLEMIFQNKKIITPDQMGMQESDVPMVISIPNQLVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1M7</accession>
    <name>A0A8J4V1M7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001454</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000034">
        <property type="protein sequence ID" value="KAF2077265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1M7"/>
    <dbReference type="OrthoDB" id="20283at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035166879" evidence="3">
        <location>
            <begin position="27"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="34"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="350" mass="39479" checksum="89C9ACABE1CF81CC" modified="2022-05-25" version="1">MKHNRRVTTLVLFLFLSTILESFVNAVNIDTLNSNNKVNNNNNVDNNNNNNNNNINSKQQQQQPPTPTPTPPYIDELYQSIYQHMESLPSVDKEGFLKRLGIELPPVNFTFDVNNSSVNLPMSIIADKLNISDVRMYSPSVVFPLTFQSTNMTYEIALQSQNLPMTLNLSPNGSEVPISLSIQGSNSIPVTLHLDRLETPLLNYIIIGLLSGLIFICLAINVYQQISINSLKKRLDRIDISDQVLYGKRYKQGNTWYCSINNQDPHLNNNNNNNSNNMNMNNNNINTIVDDVLEIRPSSPNINNNSINNQQNNQTLSNHCIMCTSCGITIIINGKTRFCFQCGNSIIRNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V212</accession>
    <name>A0A8J4V212_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MADS-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_010199</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000970">
        <property type="protein sequence ID" value="KAF2068474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V212"/>
    <dbReference type="OrthoDB" id="2284405at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00266">
        <property type="entry name" value="MADS_SRF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000002">
        <property type="entry name" value="Serum response factor b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033897">
        <property type="entry name" value="SRF-like_MADS-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="8">
        <location>
            <begin position="62"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="138"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="256"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="189"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="19"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="256"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2068474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="285" mass="31562" checksum="B2673FE0ACF11D32" modified="2022-05-25" version="1">MSLSQLQMGPNTGLFQNGNNNNPSSPTSTPSSLTPNSNEDLKKEDTSEPDSPSGESSTSKKAGRRKIKIEFIDDKSRRHITFSKRKAGIMKKAYELSTLTGTQVLLLVASETGHVYTFATAKLQPLITRPEGKNLIQSCLNTPDNPTSPTIPVPQQTPPQQNIRYNNDPLMMGGYPSNPESLYNQDNSIQDKELERRQIKEQQKQIQQQQNNSQQQNYIQPSMSSINGLDMGGLGYSPSYMGFYPTNNVQSRYIQNNSSASSSPASPNQFNYPHPIPLNQYGGPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-06-18" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3Y6</accession>
    <name>A0A8J4V3Y6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_008406</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000515">
        <property type="protein sequence ID" value="KAF2070274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="66620at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030587">
        <property type="term" value="P:sorocarp development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002639">
        <property type="entry name" value="ABC transporter G family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="456"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="488"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="539"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="566"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="596"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="703"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1178"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1211"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1254"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1291"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1321"/>
            <end position="1341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1418"/>
            <end position="1439"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="106"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="837"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="792"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="12"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="809"/>
            <end position="818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2070274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1447" mass="163419" checksum="3053855E25530A48" modified="2022-05-25" version="1">MVKPDPSDREISSPNLSIPSNSNNNNNNNNNNHNNNNNDINNNNNNHLFDSDDENESLRQMSTDINDGDDDFFPIPLEELVPNFNSGDLIDKINEKKRDNKTGTYVYVRNVTYNVKNRENKKERIDLVEDISFYLKPREMTLILGSPGCGKSTIFQLLSGLAKQHNIKGTLLFNGHPINPINHHRDVSYVCQDDIHMPTLTVKETFRFALDCQGREGLTSEDKKEIVDDTMELLGLTKARNTVVGNNLLRGISGGQKKRVTIGVNVIKGSNLILMDEPTSGLDSSTSFEIMTYLKQIVVYGYSPALVSLLQPSAQLTSLFDNLMVLTKGKICYFGPMSQALPYFKKLGFTCPKHNNPAEFFQEVVDQPERYSFIHPPKCKSSDDFVQAYRDSSLYKDLITTMDAHKDGIVDDTVNPELLVDSTEEDLPMYPTSIFYQIYICVIRGLRMTSRNYINVLARVFKGVFFGLVIGTLFWRIERNQVGGNDRFGLLFFVMTTIIFSSFATINSFFAERTVFYSQKNLHYYKTIAYFTSSVLNDIPAGLVESVIFGSLVYWLCNLHASFIRFMYFLSILILTDNLSLAFAKFCASVSPTVEIANVLGSVLLSMWLLLSGFTAPKNTIGGWWIWFYYISPYTWIFQGLVINEFTETDYYCRWYEYLPPLSDPLLNQTYPTGYSGHQTCQFTTGEQFAAAFGIDNPNGFKWTCLAIILAYIVFFYGLAFLCLKYFNFENKKSKLEVKKRKKTNKFIEPVKRAFGRGEPDLDVVEEEEEFKYGPAPQPKFHGTSYYDNNNERPAVVAPPHGGQDDTNRNYLDNQNSPLPEEHTKHSAILSETGSYLQFKKLSYSVEAKDDDGAKQRLQLLKDIDGYVVPGKMLALMGPSGAGKSTLLDVLAGRKTGGEITGEILVNGQPPDKYFNRVVSYVEQFDVLPPTQTVREAIQFSAICRLPKEVSKEERESFVDKIVEVLSLKKIANYKIGLLGNGLSISQRKRVNIGVELASNPELLFLDEPTSGLDSGDAFKVINIISNVAKKMNRTVICTIHQPSAAIFEQFDHLLLLSKGETIYFGPLGDQSSIVLDYCSKRGMDIKPHINPADFVLDIAEENKMVDGPNGEKIPFDAIKSYQESEFKTECEETLARGIIPEGYQAKHYSSPYASSWGVQFRALMRRNWLSRLRRPSIFVSNILRSILLAVVLGTLFVRMDYEQQDARNRVSLLFFSFLFAGMVGIGSIPTTVLERGVFYREVSSGFYHSTAYMLTYVIASYPFVLSTGLLYIIPTFWIAGLDSGHHSSKFWYCLFVFILAYVMYDSMALCLAVALPNEVVASTVCGVLLSLTTLFGGFMISRPNMPNGWLWAHYLDMLRYPLEAACTNEFTGLTFECTDGKGAVQVPIEGNITKPYCPITDGDAFMLTYGFHKSLRYVDIAPMMGFIIIYVGIAFISFKKVRWVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V491</accession>
    <name>A0A8J4V491_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_005375</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CENP-O/MCM21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000212">
        <property type="protein sequence ID" value="KAF2073322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V491"/>
    <dbReference type="OrthoDB" id="10050372at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031511">
        <property type="term" value="C:Mis6-Sim4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018464">
        <property type="entry name" value="CENP-O"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14582:SF1">
        <property type="entry name" value="CENTROMERE PROTEIN O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14582">
        <property type="entry name" value="INNER KINETOCHORE SUBUNIT MAL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="34"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2073322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="360" mass="42922" checksum="975C1484D1AB2065" modified="2022-05-25" version="1">MLHNNDETFEKCSGNEEIESNHETVEKEIMTTQYQLLLEKKNELLQKINEKKKQINNFNNNNNNSKTMKQVILNNINNNILNQQQAFEKYNYIEQSSRQSLDSIYSEGYKVFNQFYGFSIVTNSSHSDHFEYNHSDHLIFRFDTFFENKYNEIYFLDIKKTEGHGKVSCAIFKHTLPYWIPIDMIVGTVREEISIYDERYNRDHPSSWQICLDKQLLHESIQTILKSFVYRKEEFNKLLSYHSIAYYFHNESYSHMEVRVNYKSRTLYFKIDYSQLTDCVPSHINLKENNKVLIDSPVQVLNYELYDRYPSTSFIQMEDATEKCPPPTPTPQRDKIIYYPDSLLQLINNNIPKKSNSINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V695</accession>
    <name>A0A8J4V695_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation of fatty acids protein</fullName>
            <ecNumber evidence="10">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_006096</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>an acyl-CoA + malonyl-CoA + H(+) = a 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:50252"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:50253"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ELO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="10"/>
    <dbReference type="EMBL" id="AJWJ01000266">
        <property type="protein sequence ID" value="KAF2072599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V695"/>
    <dbReference type="OrthoDB" id="434092at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030457">
        <property type="entry name" value="ELO_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF126">
        <property type="entry name" value="ELONGATION OF VERY LONG CHAIN FATTY ACIDS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01188">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="68"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2072599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="262" mass="31070" checksum="CFBEAAA47319F36D" modified="2022-05-25" version="1">MEPILEIYNTLKERYEYYLTLRDERVKNWPLIESPLPMIYLVIGYLAFVLVGMKYMKNRKPLELKYPMIFHNLLCALISAYMTFETLYQAYINNYSFVCNPVDYSETGIGMAKILWLFYFSKSIEFMDTVFMILRGKLNQVTFLHVYHHSSIFFLWWIGVNWTAGGDAYLSAAVNSFVHTVMYTYYLLAALKIQPWWKKYLTQLQLAQFVLNVGTSIYVLSQDCPFPRWMMWAMIVYMAQMLLLFGSFYLGAYITKPKKKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6N2</accession>
    <name>A0A8J4V6N2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial genome maintenance protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_005650</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
            <location evidence="1">Mitochondrion nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MGM101 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000231">
        <property type="protein sequence ID" value="KAF2073034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6N2"/>
    <dbReference type="OrthoDB" id="17164at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000262">
        <property type="term" value="C:mitochondrial chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009446">
        <property type="entry name" value="Mgm101"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31404">
        <property type="entry name" value="MITOCHONDRIAL GENOME MAINTENANCE PROTEIN MGM101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31404:SF0">
        <property type="entry name" value="MITOCHONDRIAL GENOME MAINTENANCE PROTEIN MGM101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06420">
        <property type="entry name" value="Mgm101p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1135">Mitochondrion nucleoid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="272"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="278"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2073034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="370" mass="41400" checksum="4D8E45A49A7B3C83" modified="2022-05-25" version="1">MLNRLINRGIRGIKDVRAYNVASLSSSSSLVLNSNNSNQKLSFSTSNQEKYNNHGYGGNKVLFNDERFYSEEKYQGISKEPFSKEITDVLLANIGSEDIEIKPDGLIYLPEIKYRRILNTAFGPGGWALKPFGPPVVDNGSLIRPYALYCLGRYVAESIGEQPYNESSSFISFATATEAAKSNALVRCCKDLGIGSSLWDPTFIRQWKAQYATEKFFENQKSKEKRKFWILKNGVPNQISYPWKEIDYTSVPSQDSFTPSFVNTPQQETIYTQDSEPTTTAKSASTSASTATTTTNQSGATIENGSEETTIDIDDIVPPQMKKYAGKTWREVIGDPKGLQYLEWVVSKFDSNSEVRLLAQAMLDFARNNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V732</accession>
    <name>A0A8J4V732_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">VTT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_005164</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TMEM41 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000199">
        <property type="protein sequence ID" value="KAF2073514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V732"/>
    <dbReference type="OrthoDB" id="3364966at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045014">
        <property type="entry name" value="TM41A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="VTT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43220:SF18">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 41B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="VTT_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="VTT" evidence="8">
        <location>
            <begin position="125"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="23"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF09335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2073514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="293" mass="32774" checksum="E19316E01C9F13D3" modified="2022-05-25" version="1">MSTVNSGGNSSAQLRKRGDKEQSSTTATTTSTTKTTVTTKQDSDKEPLPLSVLLLVFVLSISIIFVLFFKFPSLSEHHKSLIRFPRNFQDVKTLGDVFSQYTDDNYFIVITTFGFIYIFLQAFSIPGSVFLSFLSGGLFGIWVGFPLVCFVATIGATLSYLISYYICRNIVKTLFPQKLQLFSEQVSKQRNNLFNYIIFLRITPFLPNWFINLASPLLDVPISTFSIGTFIGIMPATFLAVKAGLSIQEIQKPSDIFDLKSILTMALLAILSILPTLDPVKKRLDKLLNIKNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7R7</accession>
    <name>A0A8J4V7R7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nas2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_004459</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000157">
        <property type="protein sequence ID" value="KAF2074249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7R7"/>
    <dbReference type="OrthoDB" id="72325at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000107">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040815">
        <property type="entry name" value="Nas2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035269">
        <property type="entry name" value="PSMD9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12651">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12651:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18265">
        <property type="entry name" value="Nas2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nas2 N-terminal" evidence="3">
        <location>
            <begin position="14"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="98"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="109"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2074249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="253" mass="28473" checksum="C3E51BA49DB33475" modified="2022-05-25" version="1">MAESNNTIITVESVQQLIKERTSLERELESLTKFLTTGEGSTFGLHGSFVDSEGYPSPHLELIIEVKKARNRISCIQYDYKKLMKTIEQNLEILHSQSKSNPIARPPITNNNTTQQSNSNNNTNVNNNNNNNKNNHNNDNDMDITQDSIISTHKEEVKKEVEFVYLDLISPGSPSEKAGLKSGDKIYQFGSVGPIYSLDSSISYLQAIASIVRNSEDKPITIKFYRDNEKMTTILIPKKWNGQGLIGCFIKPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7X7</accession>
    <name>A0A8J4V7X7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004247</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000147">
        <property type="protein sequence ID" value="KAF2074429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7X7"/>
    <dbReference type="OrthoDB" id="437at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="93" mass="10006" checksum="52EC13BFB63AE126" modified="2022-05-25" version="1">MAPSNWQALATEAFNFLKTRYSSANIKSDFNYFLAKAKAPKEITVGDAKTVGTAAIYGVMGWTIGEAVGQSSYIGYVVGYDVNVQDAINAKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7Z5</accession>
    <name>A0A8J4V7Z5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Rab escort protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_004194</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Substrate-binding subunit of the Rab geranylgeranyltransferase (GGTase) complex. Binds unprenylated Rab proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the Rab GDI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000144">
        <property type="protein sequence ID" value="KAF2074489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7Z5"/>
    <dbReference type="OrthoDB" id="1923006at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005968">
        <property type="term" value="C:Rab-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005092">
        <property type="term" value="F:GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.405.10:FF:000020">
        <property type="entry name" value="Rab proteins geranylgeranyltransferase component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.519.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018203">
        <property type="entry name" value="GDP_dissociation_inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001738">
        <property type="entry name" value="Rab_escort"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787:SF4">
        <property type="entry name" value="CHM, RAB ESCORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787">
        <property type="entry name" value="RAB GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00996">
        <property type="entry name" value="GDI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016550">
        <property type="entry name" value="Rab_ger_ger_transf_A_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00891">
        <property type="entry name" value="RABGDIREP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="101"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="105"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR016550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2074489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="588" mass="67058" checksum="212C1C9F05282FF6" modified="2022-05-25" version="1">MEEEQRLNIKKEKETWIDVDQFDVVLLGTNFIESILAGALARAGKSVLHLDKNSFYGGFDSSFTLSQLATLLEESKDVKDPIPPYLSNVIVTDNSKIFEEEERLEQERKDKEEQEEQERKRLEQEKEKQTEQQDTKDDETKGGEGEESKGEEQQQEQTPIVKKPIKKTLHELGRFFSIDLAPTLLYSRGSMVNLLISSSASKYLEFKSLDQNYLYAKNTLYNIPSTKGSIFKDSTFSLREKRLIMKFIETIRELTQPTENEQDKQEQEKKLEELNSKHDGNFLKLVKSFGMTELVESFVLYGLSLIHENLESISLKQGVESVQLYVSSLLVYGTSPFLVPYYGCGDIPQAFCRLCAVYGGTYVLQRSVESLSFDENNILQGIKCTEGQPIKTKYFIASPAYVKTLDTQNTYPTITKRYSRFIGITNKKLNNTLEQSFITLPPNTCSNKNVINILQLDSICVPYLKGKTLYHFTTLSEGESTAEEDLKSSVDNFFANNTQEATSTDKPRLEWSSYFNFTFETLTNDIKEKNVLNKDGGVLVCSDSPIGSTIDYASQLEEAKKLFEYICPNEIFLEKVPEPEDIVWGEAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V807</accession>
    <name>A0A8J4V807_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transposase Helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001345</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000031">
        <property type="protein sequence ID" value="KAF2077342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V807"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027805">
        <property type="entry name" value="Transposase_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13613">
        <property type="entry name" value="HTH_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase Helix-turn-helix" evidence="1">
        <location>
            <begin position="41"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="121" mass="14161" checksum="C166D05F5D3DE0FF" modified="2022-05-25" version="1">MNYIKNNDLSKQLTGVPTDILNVISDAYEKIHYKQNKLSSEALLLTLPYRHYPSMLLLATIFQISKHSAHRIKNKVQTFLYTLFKPEITMMDPKRSLEEGHLFEPPKLTFISYFGWNRATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VAB3</accession>
    <name>A0A8J4VAB3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_001740</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000043">
        <property type="protein sequence ID" value="KAF2076964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VAB3"/>
    <dbReference type="OrthoDB" id="6105938at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042755">
        <property type="entry name" value="COP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44080">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE COP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44080:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE COP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="23"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2076964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="388" mass="44417" checksum="9A7F7D4007A01696" modified="2022-05-25" version="1">MQDTDHELNLLIPVKPFADEISCPICFNTIKNSFMTKCGHNFCEVCIFECINRNHRCPCCNTEATKESLVKNHQFDKIISIIESEKEESSKNYVSGIMSKKLDVNIDDNNTANSLKSPVEQVFNKYLTTSILSFEGFYQDLYKKYTENVKKIENETKNVTDKLSSGGSFNHILIEQAKAEQNIKLKKLEESFKTSSDLLINSVEEYLKKTAILPSFVNIFTAISIPDRNLSFDHISLKPTSTVSDIRAILIEKLNQRGGNYTFVSFNDSSYFIVEKPLALNDNQEIKLIDELRPLSFYNIPPGSKILLKGGVHLQGDTPKECFIKTYVAGVNDVMDYYRCEDCNYNWICKPCSEYCHSGHNLRIHIPNHKTTFACCYDSRHKKCKIIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VAK5</accession>
    <name>A0A8J4VAK5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_001438</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000034">
        <property type="protein sequence ID" value="KAF2077249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VAK5"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20617">
        <property type="entry name" value="CYP1_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000068">
        <property type="entry name" value="Probable cytochrome P450 508A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24303:SF31">
        <property type="entry name" value="CYTOCHROME P450 307A1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24303">
        <property type="entry name" value="HEME-BINDING MONOOXYGENASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12 13" id="KW-0349">Heme</keyword>
    <keyword evidence="9 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="430"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2077249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="484" mass="55842" checksum="FF35657683CA98BD" modified="2022-05-25" version="1">MSIILLILFIFISYVFLNYIKKFKNYGPKSLKGPIPLPIFGNLLSLLPLPHIPLTKYHETYGPVYRIWMGDKYTVSVNDIDIAKKIFIKNADCFMEHPETPTFKFYSCNYSNLVMGRGQNWKQRRDLVARSITKTKIKHIYTNLDQQLDGLLNCMDYHVKSGLPFTPRGNFSRFTMNNMLKLVMNDEIPYDHQGKSQGPMKDLIHQCEVLFTNLGSGNIGDFIDLLKPFLSIYYKLTNSCLPKILDIVGKVLQKHVDTFKEEYKSNPRDLFDVMLVEYDFDPLKYITIILIACDLLFAGSDTVAGSLEWANIFLCNYPEYQEKVYSELLCVVGKDRKVALSDRISTPFLNAFIKESSRFKTIGPFNIPRNCSQDIEIDDYFIPKDSQVILNFSGIAANTKYWDNPQVFDPTRHLEKNQEFSLFGYGQRQCVGSNFGQDELYLSLSNMILKYKFSSHDGNLINESEAFGLTLHPATIFKLKLEHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJK7</accession>
    <name>A0A8J4PJK7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009876</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000839">
        <property type="protein sequence ID" value="KAF2068800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJK7"/>
    <dbReference type="OrthoDB" id="21656at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051647">
        <property type="entry name" value="Mediator_comp_sub12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007:SF12">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="116"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="323"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="730"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="265"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="380"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="148"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="185"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="750"/>
            <end position="781"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="781" mass="93140" checksum="E82F100EB35D00A1" modified="2022-05-25" version="1">MSSNIQQANSSDGNRKRRNKTTTTTTTTSTSTNNNNNNNINNINVSKSKEIKNTTNYIDWKFLSLLDIDYMIKYNQIDTLEYVIELILKVNPYQFNSDPCYNKYFFTSSLLNKNSSTTKNRYRNQQQHKSNNNQNQLDTDATESDDSGYDKTTTDDQRTKSTSSSRNGRNTRFKKGFSNHQAYQEFDEDEEDDDDYEQDQEEEDNQDEEEEDEEQEEEEKEREQDYKYNHSRKNKNNSSNINKKPDISKLFKLSQLAIQYLMDGNDSSKQTIDIYTSEINELDNQISTLQQKYDNLLQEYSFLSRERELDLSLKLERLQYNQQKHNGSNNNNNNNNINNINKDYSNGNNIHLRSNSSTTSSSNTFLKRDNRFQHNQHHPINQTEQQLKYLQEEFDKLKLDSQIKAENHQKEMEKEKKKYSHANKKLEKYKKEVDKLQTFIYTNYNQDNDQNHQIMEQYNQKIQKEYLQYFQQQTFIDELNYQKQQELELIDQQPQQQHQLLIQQQQQQQQMQQQQDNEISPNKLSQSVSLINRPYKLTSHIQHNHDQIEQEREKISIQLNERLSQKGINQDEGLTKKQLEHYLLQLEMERKNDENIEEQLEMRQHLDGFIEDIITNQLYNFLIDPNNSTYSVNNTINENQRNQSNNQHNNRINQNSYQDINYWISQWLDENQLEIVVDLFKQFNIQHLTDLIDLISNQEEFNEIFENYQDIKSILSVNLEDLVLNNINNNNNNNDDIDNNSHKDNILYSNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKP6</accession>
    <name>A0A8J4PKP6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010034</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000893">
        <property type="protein sequence ID" value="KAF2068643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKP6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="84"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="247" mass="27853" checksum="990107B5C3C81AE5" modified="2022-05-25" version="1" fragment="single">YLKLNNNNNNNNNNNNNNNNNNNNNNNNNNNNNDIFDNQVTVLNNKEEEIITDDSISGDEEINDKGSGIIGTIDELRNLIHNITNSTTSPSQSTHTTLDPTSEEQHGVFYIDPVTLGAIIKDHYISDSIDLEISGEMVIASSDENKQQEEPKIKVFEKSILDHIQRKSRDRDIILGKQRTPNTTPPFSPCLSPNNQNNNDIDVSSLSEPISPRKEEEKEEEKEQQTNDQIQLENGNKENDNISSIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKR8</accession>
    <name>A0A8J4PKR8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Complex 1 LYR protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009088</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I LYR family. LYRM9 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000634">
        <property type="protein sequence ID" value="KAF2069597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKR8"/>
    <dbReference type="OrthoDB" id="190541at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20269">
        <property type="entry name" value="Complex1_LYR_LYRM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045291">
        <property type="entry name" value="Complex1_LYR_LYRM9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36758">
        <property type="entry name" value="OS01G0342800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36758:SF1">
        <property type="entry name" value="OS01G0342800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="2">
        <location>
            <begin position="11"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="74" mass="8710" checksum="7607F4E275A89802" modified="2022-05-25" version="1">MNSVAGGNKGLSLYRNIVRAINTKLPQQAQNYYWAFTREHFEGHKEETDPETIDFLVEKGYTSLRWIIKKYTNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKS9</accession>
    <name>A0A8J4PKS9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000892">
        <property type="protein sequence ID" value="KAF2068645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="24355at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035159578" description="EGF-like domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1292" mass="146251" checksum="AA46B1232D8B7963" modified="2022-05-25" version="1" fragment="single">MFKYKNFYLCCLFLALSILSTVSHAQVIESFIDYSYDTCIFKHTDYTLDLSGFYDPTNQTLAFGSNFYFNLCGQTNNKCTQFGPLALACNSNAFGERGVPFLKFSDNGITLNIPYIGMNKEIEVKVGTKLDITSIPTTNVLSVQIPRMVIKKNDPTKKTLDISGPVSIPIFTSTMKINADFIKSLDSPAIDVKIYSYEGVYLYDCNQIVLNQTQGYLSCIYSPTGREYINTLYRLEVTMGTLIGYDLFYFKQTKEISQSLFSSVDWDRCLFTNTDYSFNLSSYYNPAQPVLSVPFHDSNQEFFFNLCGTKNAQCNDDFACFNGYQFKSRNAILSIDDEGPKLLITYSNSFYPSDVQVEVKFNTKNDIYINNYNNNRIIVQSPSIAVIKSIYFGFADRQFRVPGGLFKLENLNDHLQSSPLPSIHIQISDNNGPRDCPVVEVNSGLGYITCQSNTLGTEIFSLYPTNYINLTIDTQAKGYKSIGSAYYGYGPTSDTEYIPMIESDFDRCIFRHYNYTFDLSQGAYDPSNPITTVETKTFVLCGNTIAYCGFQYCPFINFYGWTERNAYLIQNEGRYKLLIEFYAVYPYLVELDNLDSNNFDYQSRVYMYLPKMVRKQNHLLFSLKNDEILRSSTDVTIIGDFIKQIPNDSTIPIDISIQGNGVDGNYFTNPCQDNTMVIDRIQGTINCQIIPSGYEVVGKYYRVQVTMGESSGFNEIKYREPIVYSTQVMINSDYVRCIFTHKAYTIDLSDLVKDSIFNVGRTYLAICGGAFQKCSEFPDLAKYGCDFSDNSRSYFFNRRVATLKKDEEGIKLVIKYQSFQNGNEALLEIGIGTEKRVKFLQNYVFRTPQMKIKNTNLVTLQTPSLPRYAVSTLRFSSDYIKYLPANNLPPVSVKIYNSVDNTDYRECPQPIIDQSQGFISCQCTPTGKESAFTNYTVMASINTVDFYSSESLFFYAPQEISRQPMIKSNFERCIFKSNNYIFDLTQLYNPNDPVYIVDNYKFILCGDVNNKCEGDGLACNLPVRYSRREASVLVDQQAVKLLMVYYSYDYIPNPQFSVEVFPGAVGWYSNERLIIQSPLVAVRNIISFTTDYSISRASQSTMTINGDFVKGLTSDTPIKIFVRTFDDKYYSDCTQTQINSNQAVVTCQINPTGNELLDSPYQLVVDVNNTDSGSSYFYFHPKSCPSSCGKGTCDTQKGVCLCEQGYTSYDCSLKLSLKPLSNPSIIPSPIDQLNAGYQSPTSSDLVFTIDLVSLAISNSFGYILNSTPIRSISNVQSVYGTNSATFSSQSFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL07</accession>
    <name>A0A8J4PL07_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein CASP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_008818</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CASP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000583">
        <property type="protein sequence ID" value="KAF2069865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL07"/>
    <dbReference type="OrthoDB" id="10257567at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012955">
        <property type="entry name" value="CASP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057476">
        <property type="entry name" value="Cux_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14043">
        <property type="entry name" value="CCAAT DISPLACEMENT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14043:SF2">
        <property type="entry name" value="HOMEOBOX PROTEIN CUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08172">
        <property type="entry name" value="CASP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25398">
        <property type="entry name" value="CUX1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="752"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="domain" description="Cux N-terminal" evidence="14">
        <location>
            <begin position="87"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="CASP C-terminal" evidence="13">
        <location>
            <begin position="491"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="561"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="656"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="803"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="205"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="455"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="597"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="28"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="676"/>
            <end position="699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF25398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2069865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="822" mass="93834" checksum="B132BA6D632DCD56" modified="2022-05-25" version="1">MDDPVQQQQQHQQQQSIPTSPQSNVVENSNIQSFASSPSNSSAYFGSVPASAGSMTPNYSITSSNSQIPPPSSSSTTTAAAVTQSNNNNSFESILESWRVFNVEKKKSDLGDLITQIGGLKEDCLKSRKVLAKQTQDFKKVADEEKIKNFGSLLKLYQDEVDKLTKRSKFSETCFLGVYKDLNEITDPVPALTAALEEYSKVDITTKLEIENKKLLTELNEFKKEFQEIQNQEVTIRRLEDKIKEYENRFESSALEKVGIREQELRQEFSEKINLIKAREQELSRTNALLQEELNKLTIAHSQSESLLLDTKNKYEDELNIKQSEIDMLTTDFERISSTLSSIQKENLSSREESMKPLVQRVARLEYELIASGASTAKNLETISELTQQLASERESSVSFQRQLEIEREKVDKLDTHIQNNPSPADYENIKKELATLQAIVGSDEISNHPNSDQKNVFKEKNRQLENECTKLRLIVSEYETDIIKKNYDIDKLIQIKDEQYALIQKLEEDLSIKGQMDNNMMMGGNVGVHPSLISSSNNLRLSNDITELLPNNSLTLSPIVNNNNNNNNNNTNSLNNSSLTTNDLHTNSGIKDDKMLDIVTSQRDRLKLQVKDLEVEKSKLLKEVEKNTMEISSLKNDNIKLYEKIRFLQSYDKNRTNSNKRNNIDKNFDIESGLNNNSNNNNNDFNNNSGSNNSNNNYNEEKYGKLYEESINPFLSFNKKEKYRRYREMNTAERVIFNGSRFFLSNKYSRLFLFIYSIFLHFLVFFTLYRLATITTEQHIEGFNNNNNVIPQVTPLLPSQKEDGGDAGALDSLFSSSISDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLK7</accession>
    <name>A0A8J4PLK7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009855</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000831">
        <property type="protein sequence ID" value="KAF2068824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLK7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="424" mass="50221" checksum="1D264624C7247A14" modified="2022-05-25" version="1" fragment="single">FDISWFDNAVGYANKQFFRIWNIFKSRDQIIVLPNEIEFYPNSVEKWEPLNPTFGWYRTDIYIFELLVQQGYSAEFLSQTLDDAFTYYSIARSYSSLDDYKVVERLIKMGVLDCETMIYHSIEKDLHQLFKLIYPLRDKSQVQDQEILETVINTQRMELLEFLEYDATVPIDWAQINYSLNQAFLKNGYKTIQQHPDAEKVATELLETQIDRHSFENIKFMLTKFKFDCIGPFIPSILRCGDSNIVELFIQHKDTAFKDPPTRQDWERLFEDAFLHTGWDSNFSIFNSLVEHNLLPNPIASDLQKVLEEEVLSLISPRVLLLIKPLLDPIRHKFLLDAPLLHKDTQVHDFMYLIDTQIYDTVDPFVEHIIECNEQHTVQEKLEYLVCNQHRFKNKPGNTQYITNQILNTVKSPKLRSYIDKHYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLN2</accession>
    <name>A0A8J4PLN2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nodulin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_009325</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000680">
        <property type="protein sequence ID" value="KAF2069354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLN2"/>
    <dbReference type="OrthoDB" id="410267at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576:SF158">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 12-LIKE CONSERVED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576">
        <property type="entry name" value="UNCHARACTERIZED NODULIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="402"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="440"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="469"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="494"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="530"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="571"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="289"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="615"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="70"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="299"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="321"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="333"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="615"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2069354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="636" mass="70383" checksum="D0AD8B0C160FD494" modified="2022-05-25" version="1">MIQNDNNSSTEDLVNASNSSTSTFNSSVDFDDSSMNSKPTKTATEDPAFEYDEKDIELQSINNHNNNKNNDQDSSHELKKFYGPPHPLRDNEKAMKYTSLAWGAVTILISGTLYGFSLISHEIKEKLDYSQSDISEAISIGDIGIYLGASGGYFYDRLGPFYTCLLAAVLYTIGYMGCWGVVSKRLSTQSPLLLAFFLFLVGQASHATFTAAIVSNVHNFSLKHRAKLSGLLVGCFALSSGIFGIIYKSTFKKSKNVEGYLMFLAILLTSVTLIGSYVLRYIGHEDDKQTKRVKKTNDSSLSQSSQSIQEQHTNNDTSNINEKESPKTKREVGDEEEEDDNDQQDQHREVKLQSSSSSTKTSEGEGILSIHDQSNIDPFSGPNYLDGKRDISGLKLWTNIEYIITFINYFFVAGGSLMFLNNISSIVASLELNDSYSSDLVIIFSVCNLSGRVGFGLLSDFISKKYSRFFLLSFCSMTLSIVLLLFAIFMKKFFLVACVLVPIVYGGTVSLMASLISLRFGSRRFGLNFGFLNFSSASGSLVFGELSGHIYDSFANEKHNCYGEKCFRSSFLISFSCIFACTFLTLSLVYILNQRRQKAMAEIINNNINNNINNASGSGNNNTNGNSSTSDDIPIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMF6</accession>
    <name>A0A8J4PMF6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_009028</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000621">
        <property type="protein sequence ID" value="KAF2069652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMF6"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061118">
        <property type="term" value="P:regulation of positive chemotaxis to cAMP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900024">
        <property type="term" value="P:regulation of substrate adhesion-dependent cell spreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00876">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001763">
        <property type="entry name" value="Ras family gtpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2069652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="188" mass="21509" checksum="EC367CE4AC9CF288" modified="2022-05-25" version="1">MINVKIVILGAGSVGKSAITIQYVNGEFIDYYDPTIEDMYRKVIEINNEHYMLEIMDTAGTENFLVMRDLYIRNGQGFFMVYSITAKSTFYELEAIKDQISRIKDTNTCNIPMVIIGNKCDLEKDRQVKSSEGEELVKKWGGNIDFLETSAKDNTNISIAFEQLVKQIISKSPKQPTTKKKSRFCYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMX0</accession>
    <name>A0A8J4PMX0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Armadillo-like helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007826</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000439">
        <property type="protein sequence ID" value="KAF2070855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="17150at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046579">
        <property type="term" value="P:positive regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904515">
        <property type="term" value="P:positive regulation of TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037474">
        <property type="entry name" value="ScaA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37516:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37516">
        <property type="entry name" value="SCA1 COMPLEX SCAFFOLD PROTEIN SCAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="315"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="383"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1234"/>
            <end position="1310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="111"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1252"/>
            <end position="1287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1310" mass="149334" checksum="FD88CE5A7298A4D2" modified="2022-05-25" version="1">MSSNDNNKGTHRRIGINGRSKSTVSLFQINFDGPSKTLKTPQQKPYQIPSKKPEDFLTKVRDRESTGQPVYLGNDGTFYDINYVPVFKHKLPDKNSNSNSATTSPRIESTDSNNDSSNNNIDNNDSQLVDDIEDLQERLFHLYNSIPQIDEFENFFDYEQAFLGWKFAVDTIFKDSVNFEIRLPLITGRSYYRPKVSDFQKLRENSTFDYRPSVSSFSIENDSDEKSDPLNSEVDDDPIRDSAEYDLNLIKDLENASEPWDSLLVPQEPQPADYDTFEDYDDALVRWAAICSTLSFFPPHASQLRDLIPIQSISESQLNQESPETTNKYNQNEKESPNNLGRLQLSCIIKSGIKEMNFLHANNTLPTCNLILEKERLEAEKKKEAEEQKLKQQQLQQNSRQTYSPKLQMQPSIKISDETKSNVQSFVNTMLQKRLTNMSNHKSYTSPHIATIHGTFPLPLLTTSQKPTMCFKNDHLRRLDLGLKQIQDHFDCPSRIGGVSVDFEVPNHDVSFEFDRLFSDPAYQGIVMNQLRSLRTEDRYNYLYSWYHPLVPPEEHQKHKEEIDFIIMSQHRITKDKIGIQNISDILYGDMYLDKFSDLLDSSFSEDFDGGKSYATVITKCVTPENIHELLSLLENSKSPLFQAKMSFLIMNFFSGNKGSSILEKLIQDKDVKNLSTLTNSFDFLSEAPVELFPWSEETNQLVKQVLGDSIETLWKLIFIYYYLNVIGRVLEIHVHLYFSKSVINQSKVSITHSIASLLQQNTPNILDKIFTGISHRSSTVSSFCLFILLQLMHNQEGLAVHNCLKAENSNLFGRIKRLCDSKLKHVQFSSRRLFSVLGKAPWVDFAYKEYNKDLEACAGVNDFIGSDEKRPSQLFLELLLEFFTTSLDRTWEAATASLPITNTVSTPTTPPLSPVGSPPISMVSPGTSQPMITIGIKNKFSFVLDGVFFHYLLAYFFKNSKVANYQMYVITSLLAKLSKTHWRLGNINVTHNVKTSQKKWGQQNLYLSPLDIRNMSSKINDPVSDGRYSYLIKTNMLCTLRHLFKCYPAYEIIKKEDDFYSRLLSYCKDGTSSDFNKEAWRLLYQLMRYHTGTLEILSKDILSNFLELVGTSSHITVITSGLHYITKMFNIFEIESKSMKRNKADLKIIEKEMKTLNALFISKSLFIKIHMIYKKFTTRSAKEDPVYGLAFIELAKFYNTMYSPHCSKLFKATIKKPEYKDGLTKIFNMFSHPASDGEQSSSEGQGGGNIGKSSSRMDNSTGCSSPSPSTASPVSSPTPSNTNPPSKGKGDVLRGLFGSFKSPLLSSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMZ1</accession>
    <name>A0A8J4PMZ1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007859</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000443">
        <property type="protein sequence ID" value="KAF2070812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMZ1"/>
    <dbReference type="OrthoDB" id="10666913at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027844">
        <property type="entry name" value="INTS15"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14964">
        <property type="entry name" value="INTS15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="280" mass="32557" checksum="CA3040A9FBFE8769" modified="2022-05-25" version="1" fragment="single">VPCLPTLDNIMESIYNDDDIVLYSSNSKNNSNNSFKYNRYNITYYLSNIIISDHLLLTQSTNFENLPTICFKLSHQIIEIILSNYLKPNQHSWKEFINPKVYSLISDYWITLNPLLILTPVYDNGNGNSKSDQPQQHLQDLISILVFENIENDLEYYSMIETFVLSLHSILLDIFFNFPFNYTEKYTEQQRNSINLYLNNCFTTTYLENLINSINNQTTANKEDISLSIDKLTQIIQISLTPLQCLSPNIDKGQYISVLKKIIHPTSLLNTILNENIIDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN30</accession>
    <name>A0A8J4PN30_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008416</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000516">
        <property type="protein sequence ID" value="KAF2070269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN30"/>
    <dbReference type="OrthoDB" id="73919at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47800">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47800:SF5">
        <property type="entry name" value="FER-1-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="1">
        <location>
            <begin position="21"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="245" mass="27827" checksum="7EABD2E10A68A644" modified="2022-05-25" version="1">MDPSLQQQYYPQQQFTLPIMDPQHPKVLARPMGAELVGTQGLLQLRILSGENLVAADLNNKSDPFCKVRAYSLKHGPTTKYIEKTLNPIWNETISIAIQDIEKEIVVIEVIDFDSIGQNDPIGFFALDLTKLPKGIEVITRENLQDVKHGRVEIGVTAVDFGLINIPPTYIGHYIQYRQDILIGKTQKEIKSLVSEYRKDISMGRANIHEGPFIIGKLYPPEGFKLRGGWVRKDKKKHLSYSHEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNI6</accession>
    <name>A0A8J4PNI6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_008031</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ATX1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000463">
        <property type="protein sequence ID" value="KAF2070653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNI6"/>
    <dbReference type="OrthoDB" id="689350at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016531">
        <property type="term" value="F:copper chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006825">
        <property type="term" value="P:copper ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000008">
        <property type="entry name" value="Copper transport protein ATOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051881">
        <property type="entry name" value="Copper_transport_ATOX1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46365">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN ATOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46365:SF1">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN ATOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-0187">Copper transport</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="HMA" evidence="8">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2070653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="68" mass="7182" checksum="69C7DFA1122E1E6B" modified="2022-05-25" version="1">MTTYTFNVEMTCGGCSKAVTAVLSKLEGVSNIQIDLPNKKVACDSTKLNADELLTNISKIGKKCSIAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNP3</accession>
    <name>A0A8J4PNP3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007449</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000398">
        <property type="protein sequence ID" value="KAF2071232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNP3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="786"/>
            <end position="836"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="855" mass="100421" checksum="C93F592A7D3A75BF" modified="2022-05-25" version="1">MSNTSLLCKCCSPPRYYELPVLLSVCDHIVCRDTVAQFGDTYVCSLCNSISDQLDTESDRVRDILNGIERKYICKLHNQDIHEVCWTCNTFKCDFCNCNTGPHDCTALFKLDCIKVSNILHENIRKFMQDNTIKIQDNVDHYEQDIRNNNQIITRIRELKQYIKNRLDNYYLQSLETEFSVLENRLSSSINQLAETKQNIINYLNNFNTVILGKHRYLQIFDWFKEKNRVCSYKVYSSTTFKEYDTIDQLLNSYYKPHQDSLTPITPPTDHQQQQFKNTLKQCIDIKESIDTRPLLCYPIINSYKFFNLPKHPIVEPIINNQLDLFLNSKTTPNNNNYIDNVFVILNKDFDDRVTRLFYYISESKVQFKYLFFVRSLDDYKNILIKLDKNFSHSPIVGERELIDLFNSHISNLTSVLIVYHHFFYYKYKDEIQHKNSNIKTIIITYDANFKANHPNVNIFGQHVLKPLSNEGLEFLFNKLTDSIVSKDDIFYKPLMYTITRMDPEFTIEYLTEDIRNKKKTPNDIIVSKLSYDVDTISPSELKPEHIIFKFPVGEMLLSFEVNYLKSQTKQNNEWVPITIMQSPNECTDPYQLILTSEYAQIIVNNNTNGDVYNIKFDLRFKKICFNSLYRESCTNLIPLSGTFKSRSHGIQSLSLSNCFGTILYRSIQYYNRILDKIEIDAIGYSFSTNNNNNNNQNSEKRIAIRLEAGEDRINFINIEGNIHFIGDVKFFKDTDQTLIVTSNYSRFANNNQLIFKSKDNQPIPDRGSFSGNLCGDIILKDGNHLEQEIRNNNQVITRLRELKQHIKNRLNNHYLQSLETEFSVLENRLSSSINQLTETKQIYFQKMTYFTSHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNR9</accession>
    <name>A0A8J4PNR9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_008836</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000586">
        <property type="protein sequence ID" value="KAF2069846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNR9"/>
    <dbReference type="OrthoDB" id="18010at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024586">
        <property type="entry name" value="DnaJ-like_C11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052243">
        <property type="entry name" value="Mito_inner_membrane_organizer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44157">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44157:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11875">
        <property type="entry name" value="DnaJ-like_C11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="4">
        <location>
            <begin position="50"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="128"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2069846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="581" mass="66624" checksum="BA526C09F03BE302" modified="2022-05-25" version="1">MNHKHKDQNQFIHDAAAEQDNQQQQSSKRRQKRHIDNDYDDTTSVETEVDLYAVLNIPRDADEEDIKNAYKKLVITYHPDKQTSEEMKIQSQENFSLITLARDILLDDKLRAIYNEFGLRGVTDSQSIVKKYDNVENLLDAMDRIEKEKSEEKILKDFKATGQQSISLAYMHSYRYFWFKSLSASQSFSLNSKYGAFEVNPHITRKNNDATLGISARYRVPVFQNKTFIYDFEYNENTIPINRVTFHSPLAANTQGTISMTTVKHYPVMFGGSIKRVFSPQVEGIASVVITRVQKSITLDITRTVEKRILNLNLQLGTVTGGQATISREIPIGKNTGFEFGFSAYTGFNRVENGLGSVFTTIGTRVNKVLDLSFTMKYTPTRYLYIIGIHHRFQTLEIPIPIYTDISLLNSLVFFTLPSVTLSLFKLLVINPIVKLKENKRIQERKEKFASACLEARRKAEIDIKLMKSTVDKKVLQEKAKNGLVIQEALYGKLNDKAVSGADNLEFPPYINVTVPLQYLVDDSKLMLHSNKKSDLLGFWDPRIGEEKQLKVTYFFQNKLHIVVVNDNDQLMIPLRSHLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP40</accession>
    <name>A0A8J4PP40_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000447">
        <property type="protein sequence ID" value="KAF2070778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP40"/>
    <dbReference type="OrthoDB" id="21001at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071763">
        <property type="term" value="P:nuclear membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33416">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33416:SF20">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="427"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="741"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="901"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="970"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="181"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="397"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="741"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="904"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="974"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1006"/>
            <end position="1024"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1024" mass="115997" checksum="D46ED03ABC86FEE1" modified="2022-05-25" version="1">MNISLDIILNKIEELVSDSDFKDSTTFSKVRKRKNDKEEKNFLFKFNQILRQLIEYKDDRSMVHIFHLRFIHTNNHIKRSKILKLIAIITEYLRLSNKCTEGSSLGFQPLRQSYAMAAASANTFSSSNNNNNHISNNNNSNTNNSPPSSNQGSFLTLENSNNNNNNHNDDTDVNSFSPIQSGSNPSSPHSSFDVECLDPTNNININISQPNITTVNNTTVGSNNNATFSTLNFSSPLSSSISSVAASLSVSTPPLLHISPNLSSAATTPTISSTPMSISTSSSPALLSSSTIPTTKIEVTSEMKVAKELLDYLSSHQMDPDNSLIGCELNTVYLDGVFKYRSQFSFNKRTLVKMIKSWGSMKDELLLKEVRKMSSNLNNLDFKKGELVIVDCNPLSNNNNGNNNNNNSNSNNFDSDHNNEFDDDFFDLSDHDNVNNNNNNNQPSQINMSGEIDESIDITSNSNSYIFARFEEEFNRTCKLYTNPSEDTKTILSFNINNVYKLSPEIIEMTKQEKAIIKLVHDPSISFNHMLSFKERQFFNDYIESKGADLTIFEKCKNNTLSVIDLPILKSSIQLLNDLDKNLVSTPLYSNVLKMAVQESKPFLENARKTCFYIPLYLQLEEFVKNIESNREKLLSSRIRQNFIILDSCYYRVHMEKKEILEGISKNKLSQWWHSLIKEHVESFLIRLGFNIHAVPADYLEKPPQNLSTVKQNLDLLHSVLLIPNHQQVLYTQSLSQSSTPSVSSPSISSPSLSSVDHHQHTQQNQQLEPIHFKEFKRVLTILTETIYPAIEDFILKSKLVILDQFDLFNQKASVLEEKGELDLENLKQLFQKKKETLEKSKSLQIEKIIKDIEYCIEMKRLEHHYHEKTFDVVTITGLVFSRIHRLENEMRTVLENWKHLDHSNNSNNNNNGNNNNNGNNNNNNGNNNNNGKNESGGGSSKWSFSSSDFHKSINANRLFLPIQRERVKATALSNSGGSSRSTSPSPSSHSTHETKSSNSSIKSVKSVEDNDHHQNNEDMERSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP78</accession>
    <name>A0A8J4PP78_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Condensin complex subunit 1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_007476</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000401">
        <property type="protein sequence ID" value="KAF2071208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10263978at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000779">
        <property type="term" value="C:condensed chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010032">
        <property type="term" value="P:meiotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026971">
        <property type="entry name" value="CND1/NCAPD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032682">
        <property type="entry name" value="Cnd1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14222">
        <property type="entry name" value="CONDENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14222:SF1">
        <property type="entry name" value="CONDENSIN-2 COMPLEX SUBUNIT D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12717">
        <property type="entry name" value="Cnd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Condensin complex subunit 1 C-terminal" evidence="8">
        <location>
            <begin position="1062"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="90"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="248"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="587"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1401"/>
            <end position="1487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1548"/>
            <end position="1607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1653"/>
            <end position="1712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1417"/>
            <end position="1428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1429"/>
            <end position="1462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1476"/>
            <end position="1487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1548"/>
            <end position="1559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1659"/>
            <end position="1673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1686"/>
            <end position="1700"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2071208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1712" mass="193419" checksum="1AEA82CBA0273D0F" modified="2022-05-25" version="1">MSKKTTTSTVKRGSGKKKDEEMIGLPYEVLEDIGVLNISDEIINKIWRGETLQDNEKETMDFIVRKASAENLEKVLKRFKQISRDIEPMETPLYDQLPQSIDKLDINEKKKKPKKPTTKKKPPTRGGKRSAARKEESEEEEEEEEDEEDDDQDEEEKVPKSMPFNVNNNNKDVENEFVDFVRDSGFSFQSLTVLLYWLMNRHKTEQVKYIAASMYFYLFVFMRRCFHPIAFLSAIKLLSIKTNITNANNNEETQSDNKRKNRPNTKQQQDEDEDDEDDDMDQDDDDMDQDEDKDSKHDWFNSTLLLLKDLDWVLDRFSLYPYQETLVETIELLVSLSRSFHYKKTGGTKGKGKRAVMASEKEIEVIRAAHALLSNIVSNSKHGPLHSVVTMLFKELVPILTIHPGTNTYPPVVPKQLIYDRDTTLAFLKQLVDLNPQSIEEHIHTLLQHVCIRVAERAEYRSNTIQSVLSLFTMISSHSRFINNFFMAYSRNSKNNYRTFSVEVSACLLENLEQFVDKDNEEVLVPNIMGILVNRSSDKFSTVRSKALSCLSTLLEPKEESSVALKTIEKHLKNVFNLDYKTSIIPDSSSSSNDKGYVDSDDEQDDKKRKGSSNSNSIPKPSLLSFISKRSQDEKSVVRKSALQVLEVVCLQNGASKSVLDILLARTLDSSPLIKKQVIITLTNLLREHPQDNELVDTWRKAVIPLITDRESTVSEKCLESVKSIIFDSIPKLFDKKKNEIDPSYKYILRLLEDIRIDSSPYIHKVCMVMAHKKMISKEVIKNIENIIKFKSESTAASHTAGAWYMLSEIANCAPDKLDQNLIVATWNKLKDTIMSTTATGTATTPNEKEKERERELNASLLGYIVTIIESICSHFSLDVAKSLFNDIFVRAKEFRYEPMITQLFISCLVKLTVRIYRENGGAKKIESVLADWTKSILGICDEQLAVYVLPKSLTDTEKKIPVPDEQQVGLFLFTIGEIIQIHQAVIPNRLKVMIQSLIAPSLSRLVSDGTDSDPAEDIPIPTSVRAHAFITLGKLCLNDDRLAKRCIATFAKELEISDSHVIRNNVMVVMCDLCIRYTQLVDNYIPNIAMCLRDPNELVRRQTLILLTRLLQEDYVKWRGSLFFRFVEALVDPSPIVSQFANFCLLNVLQVKFGTTAAANGAGPTLNLLGSTTANVFFSHFVECIFVLNNCKSHPTYNQTNNQIFSLNIASTKHSSAQFDLTGDENRHRRMTIYETFLQNTADEHKFQLMSKLCMEVLNDVIEEKLDLKQCHGVIYDTLSILACKDIKLQSLSKLANGDTEEELNAQETALEAAKGKLITNIVKRNVIENIVPIIIELKHLLESNRSPLLRKLMLYLKELLKDYKKEVTEVLAANKQLSKEIEYDMKNFSRKAAQSPAKAVRRVSILPGTPKPDMTNFTVPQLKTPKSTSSSSTTATDSSSHTATSSATSSALKSATSSQTPLKTPFGKLGRLSITPSKISSDRQSITPFKQMMMMNGVDMSDTASSNSTPKNSTFVKPVRSARHSIAGVPINGSLGTTPFKDPITPSPFKAPAPRNKPIPLSIRFEDSDDENERDSTPLDKPTFVPVPSSIPLRLRPADNSSSPLDTKVHADIFLYSPDKPPALKKWNIKAKSSASSEDYDNDQDLLNEAMQNQFDSDQDSDYDNSDDDQETTPVIKKNVNSINNNNNNNNNNNNNNNKQKSKDCSSTNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP91</accession>
    <name>A0A8J4PP91_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CCAAT-binding factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008069</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000468">
        <property type="protein sequence ID" value="KAF2070612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP91"/>
    <dbReference type="OrthoDB" id="10263185at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030692">
        <property type="term" value="C:Noc4p-Nop14p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027193">
        <property type="entry name" value="Noc4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12455">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12455:SF0">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCAAT-binding factor" evidence="2">
        <location>
            <begin position="420"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="655" mass="75257" checksum="E36746BAB1BD6234" modified="2022-05-25" version="1">MVKESKFDKNRKFETAKTVVSKTKAASTEKVGASTPATTKSTVAAAKKSTKATVQKATVTPLEVIKNIELKVIESTENANKIIEIIKICKSNSNELEIIFQGIKSLEKIFNHLLDKDNQVMCLEFSKLKRNEKPTASKDPIIVFTQWLYKIYVSYVDMLKEFLQHEDPSIQIPVLTSMMNILLKESQLLSSLPKELFNSADQQPQQQVFYHISKSMFKTIISTMLLSNHFSSKLFEHFTNQYVSMYNDLFYYSLYSVNEICMSLVEQAKSNPEQFESTHKCSVSKFAENLFDFLTFFEPIYEAPIPDWSFWVGVPLQEFIKDSSATLKKKLKSAKFVKPASITDKDVDQLNNYWNTLTKVSSYKNIFSKAWISFLTLPLPASIYKHVLLGLPERVFPHLTDPRVLMDFFSNSYDLGGVTSILALNGLFILIHKHNLEYPDFYKKLYTLFQPGVIYAKYRARFFKLADLFLSSKFLPNYVVAAFIKRSAYLCLISPPFGSLILLPLIYSLLQRHTNCHHLINLPVNINKQQQQLQQKNNIGGLLIKSEMEKTVTSHTYGNDPFIPTEEDPAKCNALKSSLWELQILRDHYAPEVSKMAKVFDSGLKNTIDMNEFSSVSYQSMYELSFKKKSADVPLAYTLKNEFISDKDFMNDWKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPG4</accession>
    <name>A0A8J4PPG4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007654</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000420">
        <property type="protein sequence ID" value="KAF2071033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPG4"/>
    <dbReference type="OrthoDB" id="10394449at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="525" mass="59471" checksum="B6B429A890F39322" modified="2022-05-25" version="1">MDSIFYLVWRNCYIRGYIRNKVCQDLVISIDSVSELQDNYQYLSLFTNKDKKDHCIYIRININSIEELKEYIECKCNYVVNDLRINDCKIPLDLDQIPQNVDTLSLCVSGDGSMSGRLPQSLVKLIVVPSWFDDFSNSSLDTVLSNLPTSLHTISLPSYYNISSTTKIELPSSLEDLDYQTKRSNLEKLIVPPNKMYINCQANVKSLNDLQWLQSQPWIGSLKLVVPTTQTLTPGLIPSHLKKLLFYHNGAVDDGVFPHYLINLSYRSTKNPIINRLGVSQLLHQQLKHLDVSNFNQQLDKGMLPVTLETLILDSYNSPLLPDVLPTRLKYLHLNMFDHQLDVGVLPHSLLGLKLQSFTQELKPFVLPPFLQDLCLEVYTGNIVANTLPCLLNNLILYSAKGSLEHAPKMNHLSFLKVRVLDQSVATMISNTNKIFIATGSIDQDFNIQNSAIQHLELLMISKRLPLTPNLVPNQIKTLKLSGFDILSKGLIPTSCTKLRTNNLNLDLSLIPPTTTYSLKFTPNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPV4</accession>
    <name>A0A8J4PPV4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Aquaporin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_008814</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000582">
        <property type="protein sequence ID" value="KAF2069874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPV4"/>
    <dbReference type="OrthoDB" id="18905at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47002:SF2">
        <property type="entry name" value="AQUAPORIN AQPAE.A-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47002">
        <property type="entry name" value="AQUAPORIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="244"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="106"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="106"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="119"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2069874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="414" mass="45644" checksum="AD25F0D88E1F5190" modified="2022-05-25" version="1">MTNRYSITNCNDDININNNNNNNNNSNNNNNNNYHNHDDNMIRYGSNRLRQRLRRRSGGKIEAFNKKALSRNFLGGVVQYNSPMQQGFSDVEPFLDEDDQQQILSPSRLQSPRLQSDIAQQENQTPQTPQSTTATIHIEQKLKRPTFKQKLTYFIGVHEWKSNLLKAGIIEFIDFNIDSPVIQNNINSNPTLVSSYKVPITIGVLQIFILGFTILACGPSSGGHLNPVFTITTMLSGFCSLSRCVVYVVMQLVGSIVGSAMAYAVIPYHIAIRTQLGGCSYGAGITNGGAVASEFMFSFFNLFIAFGTVLDPRQKNHIMGAPFIVSLCLAISIIAASGISGTTAGPGYNIARCFGPAVIIGNLQNNFWPFVVGPLIASFAQGVLMFFLNPVTFYYSYTMDDYRRGCDAGGIPTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ48</accession>
    <name>A0A8J4PQ48_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_007060</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000356">
        <property type="protein sequence ID" value="KAF2071634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ48"/>
    <dbReference type="OrthoDB" id="265044at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061118">
        <property type="term" value="P:regulation of positive chemotaxis to cAMP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900024">
        <property type="term" value="P:regulation of substrate adhesion-dependent cell spreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00876">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001763">
        <property type="entry name" value="Ras family gtpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="229"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="184"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="229"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="259"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2071634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="270" mass="29431" checksum="8792614974E00A6D" modified="2022-05-25" version="1">MNNLKVVVLGASGVGKTSLTTRFVNGEFVENYDPTIEDLYRKVIETNKGENYMMEIMDISGTERFLAMRDLYIRNAQAFILVYSITSRVSLLELENIKNLIVQVKEKPLSTIPIAVLGNKCDLEEHRSVSSEEGESLCKKWGVGDFLETSAKIEMNIQSAFDCLIKQVASKPQYINSPKKSKNQNHSSLNSSLSNSGSSASLSNLSNLSNSSSSNSSIASMSMTSSNTSISSLSKLSRSPSSSSISKKSKSSSTSSSSKKLKIKNKCSIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ66</accession>
    <name>A0A8J4PQ66_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007840</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000441">
        <property type="protein sequence ID" value="KAF2070836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ66"/>
    <dbReference type="OrthoDB" id="21373at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012479">
        <property type="entry name" value="SAP30BP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13464:SF0">
        <property type="entry name" value="SAP30-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13464">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR PROTEIN HCNGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07818">
        <property type="entry name" value="HCNGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="168"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="297"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="338"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="370"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="399" mass="45360" checksum="666D5CAAF78A1A42" modified="2022-05-25" version="1">MSLSLLSQYGDSSSEDEQEQQQEKQEEKINNNNSNKNNQPQQEEEEDDEDNKLLNISKVNDKPYGLLITKENQPEIYDKEMWKKDNNINEFEYPSNDNDNSIIHTKLENTEQENNLDKNQCEPDTPTDIRSPVEISTPPVSKHPDFDNLYPKSINSPLGIGTPPHNQSSNSSSSSSNTSTTTTKTKQIDLSFIPPQPKGQVDSVILEKILRYHKMKQEGISINENLRNSQAFKNPSILEKLIVFCEIKEKGSNFNPQVFNPYAYTPDEYYLALNEKQKESELKRESERINRMKIDFTSPSSSLSSTTTTTTSSSGVDDKPKEKEKKKKFNKWEKAKYKAQQQKQQQNQVPQRGAVISAAPTTNVKKRDSATSTTSAVSSITTTTTAQQQPAHKRFRINP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQS0</accession>
    <name>A0A8J4PQS0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like-conjugating enzyme ATG10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Autophagy-related protein 10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_008187</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATG10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000484">
        <property type="protein sequence ID" value="KAF2070489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQS0"/>
    <dbReference type="OrthoDB" id="4089664at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061651">
        <property type="term" value="F:Atg12 conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032446">
        <property type="term" value="P:protein modification by small protein conjugation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007135">
        <property type="entry name" value="Atg3/Atg10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14957">
        <property type="entry name" value="UBIQUITIN-LIKE-CONJUGATING ENZYME ATG10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14957:SF1">
        <property type="entry name" value="UBIQUITIN-LIKE-CONJUGATING ENZYME ATG10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03987">
        <property type="entry name" value="Autophagy_act_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0072">Autophagy</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2070489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="231" mass="26879" checksum="698852F096E6076F" modified="2022-05-25" version="1">MTSFQEFKNQALSLINGWNEWCWVTHRQRVDEDGYLVLKLYKNKANCILTSTTSSSIRNDDNQIEDNDIDMSIVDSSVNSNNNNNNNSVDQDTDIHIYEYHVIFSRSYQVPVLYLNVYKKDSTVLSWSDIWNTLPLSKYDSSSHNIVPYFSQVEHPILGIPFYQLHPCETPNLMKQILSVSKEEHDDEDNNNNNDTIENLQQYFLSSWLSIVSPLVGINIPLHFLKYMSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQS6</accession>
    <name>A0A8J4PQS6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zinc-finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_007460</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000399">
        <property type="protein sequence ID" value="KAF2071226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQS6"/>
    <dbReference type="OrthoDB" id="298344at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018866">
        <property type="entry name" value="Znf-4CXXC_R1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10497">
        <property type="entry name" value="zf-4CXXC_R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zinc-finger" evidence="6">
        <location>
            <begin position="471"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="203"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="310"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="358"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="426"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="546"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="601"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="47"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="69"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="227"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="263"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="310"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="369"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="387"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="546"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="625"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="638"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2071226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="690" mass="77113" checksum="5D8249945F510555" modified="2022-05-25" version="1">MSSEDEVMNNGIEGTGSSSSAESMTVSPMSPDMGNGSSGGNGYNGNHGHHHQQHHHYSSRGGVPPPSSIIPTTSPTLSTSNPIKVPLINKLHSHVMSSNIESSPVSMFVNSSVFDSPSLHKTPSSSMGTSFLPQTPTSSFLDSHRFDTSLNSPTRMFFDFNNIYSKSPSSLLPHPLLPHTPRGTRIIDGGEEDISIISNINNALRPSTSPVSGKMSFRGDVHLPPGHHSSTAQSHQQHLSSHHQHSSHSPHHSQHHNSHQHHSQQQQQQQQQQNNQQSNGVDKNGNLFSTISSLSSTLHSMNHHTTSALASSANMEQASMSSEGDYENNSCSTPSSSHSEIEVVAKDFQMTDSEENNNFSSIAYNNSHSNHHHHHHHSHNNHHHTHNNSNLNHSQYNSQYNNNNNQNNNQYQPTLVNITAINNQNQHQFNNNHNSNNSNSNNNSNCTITTHYSQEDSNKKKKRKKDFDDGGRWCHQCKALKYQYIQCQALELGKLKCNKRFCSACLLKHYNKTVAELKSESAAWECPFCLNTCICAACKRKRGTKEENEKLEEEHLQRSTTSKKEKKPKAVPIKRSAPIDFQASLDTSINNNINSLLQRREYEKSKESENQTVDVMNGGNHQDEQQQQQQYHQQQQESDGDEDFDDGDVVDDEQDQDDDMGTGSKQKPKKLVKKHNDPITIGTRASSRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR21</accession>
    <name>A0A8J4PR21_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">S60 ribosomal protein L21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_006111</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000267">
        <property type="protein sequence ID" value="KAF2072588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR21"/>
    <dbReference type="OrthoDB" id="1539250at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.70:FF:000001">
        <property type="entry name" value="60S ribosomal protein L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3260:FF:000002">
        <property type="entry name" value="60S ribosomal protein L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.70">
        <property type="entry name" value="Ribosomal protein L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001147">
        <property type="entry name" value="Ribosomal_eL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018259">
        <property type="entry name" value="Ribosomal_eL21_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036948">
        <property type="entry name" value="Ribosomal_eL21_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20981">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01157">
        <property type="entry name" value="Ribosomal_L21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01171">
        <property type="entry name" value="RIBOSOMAL_L21E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2072588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="160" mass="18338" checksum="DAD7CA070C1D8F56" modified="2022-05-25" version="1">MPHSFGLRARTRYLFSRGFRNHGLLKTTTFLRTFRLGDYVDIKSNGNVHKGMPHKFYHGRTGRVWNVTPRSIGVIINKRVGPRIIAKKIHVRIEHVVPSKSMAAHNKRIEENKKKLAEAKKAGTFVSLKRQPAAPKNSFFVNPRNSEVETIAPLKYELLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR49</accession>
    <name>A0A8J4PR49_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007197</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000370">
        <property type="protein sequence ID" value="KAF2071481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR49"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="179" mass="20471" checksum="5C95981DE7D061E7" modified="2022-05-25" version="1">MNATAIYFFNFDDKSKRVDNSNNNSNSNSSYNNNSNNSINNNSSNNNKNNKYSSDLSTEKRETPHQQQEIETDNFQILYTNPITPNFTNSTPTSISITLPQKVKTQFPNLNSITLNYNTFIKVENLNQNQLNELNNDHLLNFNVYTLDQGIVFISYPILSYQFVCLLTIVNKTTSLACK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR51</accession>
    <name>A0A8J4PR51_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytoplasmic tRNA 2-thiolation protein 1</fullName>
            <ecNumber evidence="6">2.7.7.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Cytoplasmic tRNA adenylyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_006942</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="6"/>
    <dbReference type="EMBL" id="AJWJ01000343">
        <property type="protein sequence ID" value="KAF2071737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR51"/>
    <dbReference type="OrthoDB" id="198857at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002144">
        <property type="term" value="C:cytosolic tRNA wobble base thiouridylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01713">
        <property type="entry name" value="CTU1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000054">
        <property type="entry name" value="Cytoplasmic tRNA 2-thiolation protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03053">
        <property type="entry name" value="CTU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056369">
        <property type="entry name" value="CTU1-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032442">
        <property type="entry name" value="CTU1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000541">
        <property type="entry name" value="Ncs6/Tuc1/Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020554">
        <property type="entry name" value="UPF0021_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00269">
        <property type="entry name" value="TIGR00269 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807">
        <property type="entry name" value="ATPASES OF THE PP SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807:SF12">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16503">
        <property type="entry name" value="zn-ribbon_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01263">
        <property type="entry name" value="UPF0021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2 6" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal" evidence="9">
        <location>
            <begin position="75"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Cytoplasmic tRNA 2-thiolation protein 1 C-terminal" evidence="10">
        <location>
            <begin position="302"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="341"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="346"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="78"/>
            <end position="80"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR004976-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2071737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="367" mass="41640" checksum="44FEB30913F4EC13" modified="2022-05-25" version="1">MDNTTPTTTTNVENSTPIKKKSGGKLCELCNVARPVLKRPKTGQMICKECFYTVFEDEIHHTIVSNNLFKRGDRVAIGASGGKDSTVLAEVMTLLNKKYDYGLDLFLLSIDEGITGYRDDSLETVKRNQQQYQIPLKILSYKELYNWTMDEIVKEIGLKGNCTFCGVFRRQALDRGAVILKANKIVTGHNADDIAETVLMNLLRGDIPRLQRCVNIVTGSEGALPRSKPFKYTYQKEIVMYAYFKKLDYFSTECIYAPNAYRGHARDFLKDLEAIRPSVIIDIIHSAENFHFREENKMPVQRNCIQCGYICSNDVCMACDLLKNLNSGLAKVKLSINLKRKDKDNNNNNNSNNNNTTLSNQLVIEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRG6</accession>
    <name>A0A8J4PRG6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Paired amphipathic helix containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_006042</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000261">
        <property type="protein sequence ID" value="KAF2072640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRG6"/>
    <dbReference type="OrthoDB" id="10265969at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000003">
        <property type="entry name" value="Paired amphipathic helix SIN3-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1160.11">
        <property type="entry name" value="Paired amphipathic helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003822">
        <property type="entry name" value="PAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036600">
        <property type="entry name" value="PAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039774">
        <property type="entry name" value="Sin3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346:SF0">
        <property type="entry name" value="SIN3A, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346">
        <property type="entry name" value="SIN3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02671">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47762">
        <property type="entry name" value="PAH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51477">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="163"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="3"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="203"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2072640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="313" mass="36994" checksum="21892AADF7D81ADE" modified="2022-05-25" version="1">MIHSNKSNTREDLEKDNERLQKENWELKNYFKVGYSNTVNNSLYFQLETSNLENDKLKQENKAIKQHLKKLSEENDNHNNKMDILFNKIESLANINDKLNIRIENYSNKMDLLSEKIESMEEAIDTKNTNIDYVNLENLNNKIDLLLNKIESIERVNEKLLNTPYSPQHHYGGKQQQPSPSDIKQPPQGQLTQQIHQHLNQLIQIQQQHLQQQNQQQQKLQQLQQLQLASEAAEKKKALEYVKKVKDRFSEQPNNYKQFLKILNNYHKESQTIREVYEKVTELFSMHRDLLDEFTQFLPKTAASVPPTQPTIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRR2</accession>
    <name>A0A8J4PRR2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cation efflux protein cytoplasmic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_007540</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000409">
        <property type="protein sequence ID" value="KAF2071134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRR2"/>
    <dbReference type="OrthoDB" id="78296at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1350:FF:000001">
        <property type="entry name" value="Metal tolerance protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000005">
        <property type="entry name" value="Putative Cation diffusion facilitator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1350">
        <property type="entry name" value="Cation efflux protein, cytoplasmic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050291">
        <property type="entry name" value="CDF_Transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840:SF13">
        <property type="entry name" value="CATION EFFLUX PROTEIN CYTOPLASMIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840">
        <property type="entry name" value="MITOCHONDRIAL METAL TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="Cation efflux protein cytoplasmic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="241"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="9">
        <location>
            <begin position="177"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein cytoplasmic" evidence="10">
        <location>
            <begin position="372"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="60"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2071134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="458" mass="52091" checksum="37FCF8AB07EE0683" modified="2022-05-25" version="1">MLHQYSTYSNDNDDDDDGQDTVHGDLEDISINDDNDQYTDEGIKTPLFNHNIKIVERFYQKEQDSDDDDTQDTRSSSITIDVMPSAAYSASPSPSTIETSTLTYRKHVKTSEELRRIIESTRVNDDELKYKSKSQRKFYKKQNELIDQILTPLDQLAPNQQETQDNDFKVKVAINGSLLVNIVLFSMQITAAIITGSLTLLATSVDAFMDLLSGFILFMTERAKKKKNYFDYPTGKSRMEPVGIIIFASLMSTVSVNLIVEGCTSLIKQNDKHIDLDILPICFVVFAIACKVIMYFYCRVLTHSSSCMILATDHRNDVTVNSFGLVMAILGDHVVWWLDPTGALIVALIILRSWTSEAYEQIQLLVGKTASPEILQKLTYIAFTHSPEILKVDTCRAFYFGNNLFVEVDIVLPRDMPLHKTHDIGESLQEKLELLGEVERAFVHVDYDYKHKPEHKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRZ6</accession>
    <name>A0A8J4PRZ6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006464</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000299">
        <property type="protein sequence ID" value="KAF2072219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRZ6"/>
    <dbReference type="OrthoDB" id="10039976at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053144">
        <property type="entry name" value="Acetyltransferase_Butenolide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43233">
        <property type="entry name" value="FAMILY N-ACETYLTRANSFERASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G03350)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43233:SF1">
        <property type="entry name" value="FAMILY N-ACETYLTRANSFERASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G03350)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13673">
        <property type="entry name" value="Acetyltransf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="46"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="146" mass="16419" checksum="A471093FEFF58F75" modified="2022-05-25" version="1">MDTTQQPQSFDINQVEYKVNEPITEDQFKTLLNETTLGARRPLSNDKAVSAMLKHADILITAWVGTRLVGVARSLTDYEYCCYLSDLAVSIPKCGIGKELIKQTCKQLSPSCRVILLSAPQAVEYYPKVGFQQHNSCWTELAEKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS20</accession>
    <name>A0A8J4PS20_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_006571</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000309">
        <property type="protein sequence ID" value="KAF2072126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS20"/>
    <dbReference type="OrthoDB" id="10263353at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02669">
        <property type="entry name" value="Peptidase_C19M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000068">
        <property type="entry name" value="U4/U6.U5 tri-snRNP-associated protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033809">
        <property type="entry name" value="USP39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF16">
        <property type="entry name" value="U4_U6.U5 TRI-SNRNP-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="13">
        <location>
            <begin position="45"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="12">
        <location>
            <begin position="167"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="297"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="17"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2072126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="492" mass="56888" checksum="103FB607E1D974BA" modified="2022-05-25" version="1">MENNTNNTIDKKRSIEEDQENNNEQDDEFDVASMRSKKKQNVARKDCPYLDTVNRNILDFDFEKICSISFHNHNVYACLVCGKYFQGKSQDSHANLHCLQTNHHVFINLHNEKIYCLPDDYEVIDSSLDDIKYLLNPTYTKQQIENLDKNSKYSRALDATQYLPGIIGLNNIKNASFVNVIIQSLARIPYLRDYFLDLNNIKNNKSILINSFSELLRKLWNPKNFKTQLSPHEFLQAIQNKSNKKFSIGVSSDPLEFLQWLLNTFHTELGGTKKPKSSIIQKAFSGLVEITTETPIKKKSTDDNNDKDEKQDDDKTTTATKTTITTQPFLYLLLDLPPKPVFKDEMDKSIIPQVPLFNLLSKYDGVTPTTLLNGEVKRYKLLELPNYLILCVKRFSKNNFFLEKEPTIVNFPLKNLDLTDYLPKNTPASKYNLLSTIKHEGQPNSGTFSVYVWHKGSDKWFEIQDLTYKETIPQLIAVSECYLLIYEKVVNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSB3</accession>
    <name>A0A8J4PSB3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_006398</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000293">
        <property type="protein sequence ID" value="KAF2072291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSB3"/>
    <dbReference type="OrthoDB" id="25146at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46115">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2072291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="111" mass="12073" checksum="A611F7C1DF84A18D" modified="2022-05-25" version="1">MAGEYLEPKNTAEYNAFFESNKGKPMLVYLTATWCGPCRAIAPVFTRLAGEYPTIVFIKVDVDNCAEVPIVQSVTGVPSFYGYDKSGNSIDNFAGANPAKVKELAEKLKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSJ8</accession>
    <name>A0A8J4PSJ8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_006235</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000278">
        <property type="protein sequence ID" value="KAF2072459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSJ8"/>
    <dbReference type="OrthoDB" id="20849at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046975">
        <property type="term" value="F:histone H3K36 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19172">
        <property type="entry name" value="SET_SETD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006560">
        <property type="entry name" value="AWS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050777">
        <property type="entry name" value="SET2_Histone-Lys_MeTrsfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044437">
        <property type="entry name" value="SETD2/Set2_SET"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22884">
        <property type="entry name" value="SET DOMAIN PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17907">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00570">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00508">
        <property type="entry name" value="PostSET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51215">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AWS" evidence="11">
        <location>
            <begin position="307"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="9">
        <location>
            <begin position="355"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="10">
        <location>
            <begin position="477"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="505"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="12"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="76"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="108"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="139"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="183"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="193"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="241"/>
            <end position="251"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="505"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2072459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="657" mass="73912" checksum="DC6ECAF77D436A73" modified="2022-05-25" version="1">MNKLNRIEAIKQQKQQEQQQQQQQQQKTFDNENLKTNNNDSDNLKRNDNSNSLINDHRDLKKVKLDTPPTSPPTTDKFKVNDIPRPPIKKDLINNNNNNDNDKPKSPPSASLSSLYNSKSFAIPLKNNNGTTTPPNGDNKNERNPSNKKLLEQPNKLNKTSNNSHSSSHSPNNNSSKKKNDESNSDDSDSYSDSDSSSYSDSDSDSSYSGSQSSSSDEYTSSESEDEKKSPNKNKRSIVKSSTTTSTTTSTLPKKVTTEYITDLISQGKTPISKIAVLIQYSREKNFKFLSTNLYLERRERILDDDDDIDICNCLNIDGDCGEECINRKSYIECDVKHCDVVGCRNQRFQRKLYSPIEPFHAGPKGWGLRATQDIKKKDFIIEYCGEVITKATCINRMQKAENEKFFYFLTLDAKECLDASKKGNHARFINHSCDPNCETQKWNVGGEVKIGIFAIKDIPNGTELTFDYNYERFGASKQICYCGSSNCRGYLGEKSSKKAIISSSTLNNNNSNNNGSNLNGSASNGLSSSTSTSTSSNGNGSTYLNKSKSNKNQLVPLSEQMLVFNHQNHAKFLLNEKVDDYHYQSELSAESENHIIVSKKLFLSRNVRMLKLYYLNFYKSCLGSTDVNGDTDEKKSNVVKRRSLSQVINDLKKQVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSM1</accession>
    <name>A0A8J4PSM1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004922</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000183">
        <property type="protein sequence ID" value="KAF2073788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSM1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="161" mass="18383" checksum="40A0C28C4FD1F7D5" modified="2022-05-25" version="1" fragment="single">KEKIKAKQEKESKVELSPDLAHSNNSINSYETDEIEDEEFIITNEPSLNVSNGETPTIQSQQKDYKKEYEALAHLYLEQSSKFKIFVSRLTEIETYFAEFNNSLYSKNMKAEALGLGIAEILGVGLTDLSLSQLTELENIHKESIKEIETLKQNYTSNLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSP3</accession>
    <name>A0A8J4PSP3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_005597</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000227">
        <property type="protein sequence ID" value="KAF2073103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSP3"/>
    <dbReference type="OrthoDB" id="185175at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032425">
        <property type="entry name" value="FERM_f0"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044785">
        <property type="entry name" value="RopGAP1-5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23177">
        <property type="entry name" value="MKIAA1688 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23177:SF35">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN GACA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16511">
        <property type="entry name" value="FERM_f0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="3">
        <location>
            <begin position="712"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="222"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="267"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="458"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="269"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="299"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="468"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2073103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="962" mass="107714" checksum="51F9BC4299E63752" modified="2022-05-25" version="1">MVIENVKERIILTVKIPDLGSTKKILFDRVDTIKDAVMLIIEKLPPGALDPIDYNIYLPQKSQWCKLDARFNKYQFKENQEIEFKRDTRGGVTHMLSNVGNLLTKPYRTVYIKLPEIMPVGMVSCPPSPSPSSPSPITIGAEFITQHHSNSNPLSLSSTPISAVTPILNSSRARTSSLSSINEFNQSRSSSLCSSAISSASLTSSTNSKPILIPKLNLNDVSVSQSQSQSQNNSPTSSLSSSPRTNYPPPFEQKKSLLKIIGLSPRTQSSNSLSMSTGSMSNPNNSNSNNNSNSSPSSLVSTPRNIGTYIKSNNNESATKVESFDFDDDITYKEMLIKIFQRYQHIDKELLEDYSLVTKDGVWITEKSKTIVELNIKHMDELEFKRMTQKVKIIFLNRELVLNLNPNDTIKEINHKIIVNYLPSLIKLNRSNSILSSNSNNRASLSLEPIIIDDFNSNSTATDRTRSNSESNSPLSFSFNSSNNIGSSQQQNVEMYIKRKGSLNNGQSPVVDNIRISSDKAFTFQIGNNANDLPNELLEFKDFKLHLCSRNINPNINFKHDLLLDEKRTLSSFSFWNNIKLHFKNSTKGHHVDSKKIAPSNPYYLSIESDPLNASFVLELDSSLVISDILKSFIKSLTLNDNLKIVDSLEEYGLYFDTINGGGKREGIEYGGSLYLDPIKTLSHYSMIEPMDKLIFKRTNSIFGVDPSSMPMKLDPVTGFNIPTLLLDLKAKFIQMDGFSSDGVFRVNNYSETSFNEIIRCFNSQTLLEEKNNIFIDPNSIASFIKRWYIKLPKKIYSTLDDETLLYASTSESAAEHALESIVEPYRSLLLWLVRFLSEVAQSAAINKMSAKNLAIAIGPILVSQYSRLNEQPHHHQPSHQLHHQKRNSANLEKLHQSTLFLQHLIKLNLRENGFIPLSSSSISANTSSFISMGSVHSSNSSPISSSLISRSPSLNDIVEEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSZ1</accession>
    <name>A0A8J4PSZ1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006252</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000280">
        <property type="protein sequence ID" value="KAF2072440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSZ1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2072440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="748" mass="88061" checksum="17D76A6BE865F934" modified="2022-05-25" version="1">MSRDIDILYRDVFANKYISRQIFRHVQEEQIDRYSLKYDDVVDMGWMVRYGHLGLAREKCRDKNKEYFISYKDLFSVVANADTELFVMCFEKHKYQLLGYYCEQPLMILDSDTKQEQIKNTEVIKYLYESGYAVDLSDLDVSVMDVSVVRYLLENGWLVATQEFLMGLQEQVNCPKEMIELVIQYTPLPLSLSNSIKLLGQNILSPHPNHFDLLFPHLDQGATCFTYDTSLNYDQAIALIKVRSAQVSKLWKVSEKLPFEIDQDCKGLPEEFYLWYDKGDDAFFSLLDGVSYIIAVSEDNIIFSPNDNDTQEIDMRDFIRKTCCIKASIHVIKFFFDQGYHMIFRLDQSQMDDMFFKELIKKTSDQDRSLIAQFTTDNYEKIVTKKDVLKACCRYRHLENFHYYYQLWKDSLDTESLIPKLFKEAVDDRQVQKAIESYGYKLSDFHDSCFSYLSLYSFRYHQDDLDRFLGKINDKRRLDLCCSEFISRAFNQNNYPTMKYMFESNKFKFPPLDRYVYNILGSTHNLDLMDYISSNRSTCFINPTDKIVNKFFACVFDEACQILNLPLIKHLLAKYVDIEKITKKQLKLISPYFGPNTEISAVIDNFNEFAFLVDKGTYTSIDPFIESLCQLFYGEDQEDEDEEVPQFNTNLLFIEYLDKNLVQQPVSFQPLFDRIICDLPLIHTYSPLLGALVRRYRCVLNDSHYPFLAKEKDGLTLFGQVPDSIFLSDKYKRARAIKEKRPSKKLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTM7</accession>
    <name>A0A8J4PTM7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000263">
        <property type="protein sequence ID" value="KAF2072624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTM7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16148:SF14">
        <property type="entry name" value="MYND-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16148">
        <property type="entry name" value="NF-KAPPA-B-REPRESSING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="626"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="627"/>
            <end position="664"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="957" mass="111537" checksum="47A3E7A44A7663C7" modified="2022-05-25" version="1">MGLVISKFFETNFEEQSKSFNVGLTYTFADIVQTIQESSTIPEFVHNLFSKINRFELDVYYDNPTFQSCTYASKNANDFKYFITCTKCKTTPLCLYCFSTSMHPIGCKGKFSIEQSSLQRCCCGTNDDYSCPHHSSYVKSVFKAGTFSQHVNYINVTADTHAFFRLLLLYIGGTVRNSASLAQLKETKVLDLLEYLATLSSKSKILKKIITDEFFGIDQPRDNHNEKEKQLYSPYKFELQDYYNVSSMQRYGCVLYMETRGEIYAAHLSFLIQMFRTSYRFKSHFFRYTFEPIFQGEREIPDTLWGLRPILLYEFFVDLNIVRSHITKMNLICHSFDKVGFNLKTMVDPYRKIDIYRNESCLQYLIHAFRNSEDIRRKPFKNEAARLLMLVDFIDLLSVSEKSVYSMSSNGRLPSDIGWYADEAISELIHLLQYVEPILPFLCEAQVKDNQRGIRSKFFFAKQILIASLSIKSELKHARFLSDTVHMCSTFPLACRLLNYLHPHDKKLNDNPMYRGKAALYPVQDMAVFGLLSALQIQVSHDFRSFCFHYFRRIEPIHTTYKLLIIILQYRYSSKTDQDDIIYHFVNLIHRGIPIPPTTGLSNSLFKFIAPQQILKSLDLISTLSTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNQNNNNVSFRIGEPNRYIFNNETSTILKNDDFLVRRSPTPPREFDSPTNTRANNRVLKEEYKHLIDPYYAWISKDYLIRHPPNATDKKKLFGEVITIEPPTKKQHFQFLEQEPVKLFFLLRTTYFYTTLFVELKNAIGEKAIFKDDYYSIMPEIKPEHKVSVIQDIMYLLAWCIQNYYDRPLFEGTLDSALNQSVRMAKKNDTKNLDEFFANQSLTPFDELNASPIVNIVKKNNISSGGYKSIFEILKSPQLSKALGSYQKIRTFILNEFVKIDPRFKNLIENNNNNNSNNNNSRKTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUH1</accession>
    <name>A0A8J4PUH1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008886</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000594">
        <property type="protein sequence ID" value="KAF2069791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUH1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="77" mass="8475" checksum="70D99E9E508DB2F2" modified="2022-05-25" version="1" fragment="single">MATLLYQQQQQYTYNQYDDDYSSDEEYGQSEIYDLQDRLEKRIGGGARSCGSGSTSAAASGAKKQTVRKTSKASTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUJ1</accession>
    <name>A0A8J4PUJ1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">tRNA (adenine(58)-N(1))-methyltransferase</fullName>
            <ecNumber evidence="2 8">2.1.1.220</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_004964</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>adenosine(58) in tRNA + S-adenosyl-L-methionine = N(1)-methyladenosine(58) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43152"/>
            <dbReference type="Rhea" id="RHEA-COMP:10365"/>
            <dbReference type="Rhea" id="RHEA-COMP:10366"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74491"/>
            <dbReference type="EC" id="2.1.1.220"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM61 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.220" evidence="2 8"/>
    <dbReference type="EMBL" id="AJWJ01000186">
        <property type="protein sequence ID" value="KAF2073731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUJ1"/>
    <dbReference type="OrthoDB" id="1925287at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160107">
        <property type="term" value="F:tRNA (adenine(58)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049470">
        <property type="entry name" value="TRM61_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014816">
        <property type="entry name" value="tRNA_MeTrfase_Gcd14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133:SF2">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE CATALYTIC SUBUNIT TRMT61A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08704">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017269">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51620">
        <property type="entry name" value="SAM_TRM61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 8" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61 C-terminal" evidence="11">
        <location>
            <begin position="63"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="253"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="255"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="113"/>
            <end position="116"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR017269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR017269-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2073731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="331" mass="37044" checksum="BDB739A0C819DF17" modified="2022-05-25" version="1">MLSQKKIIEDGDRVVIYAGKDKMQVVTIDAKSGEYCSKFGNYKHKNLIGKEIGTKIQSDNNNGFVHVLSLTSELWSIALDHRTQILFNLDISTIIFNLELKNGSKVVESGTGSGSLSSSIARTIAPNGHLYTFEFHEERAEAARKDFRDNRIDKHITVTHRDACKQGFRLDHIEGMVGSIDAVFLDLPSPWEAIDHAIAVMHEGAMLCSFSPCIEQVQNTCLKLAASQFQEITTIEVLIRTFDTRLQEFEELNLSNPYTSTTTTTPPTTETNTTTSTSEPKAKKKWNQNKRETFEIGGIQGLKKDSLLSKPISEARGHTGYLTFARWVPEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUT0</accession>
    <name>A0A8J4PUT0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_005114</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000196">
        <property type="protein sequence ID" value="KAF2073565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUT0"/>
    <dbReference type="OrthoDB" id="10254455at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001054">
        <property type="entry name" value="ATPase, AAA family, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF117">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="306"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="585"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2073565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="817" mass="91844" checksum="A686DF494B258564" modified="2022-05-25" version="1">MTLATTMIPVLCKVKVLLPLFIDIAREALLCNKVNDSIHSNIHRVYISPSDVSILTRSSTVAAAGSQNNVCVDSRSHFLVLYSDSTCSVVIAVCQFVIEPKLLERDVRQTSNSNNDSSTTNSNITIHFFKEEIDLLKLDIDKQNSFHALFVPKYRFSSIDTIYLSNIIDDKNDYQSEFDYQWARVGLKVGSTLNNNGGVVNLFQRLGKINKYQYKLLLSDNNSSNTISDQSSSVIKEYSIITNQTKIIYNNSNNNNSNSMKDLNIFDKQTYNRIKSIISIYFNISSNSSNSNNNSNIIYNKLNISKPQSLLLHGPISCGKSTLIDQICREFNIKLIKVNLSDIIKYQPNSKGLQIKYTQAKQQLKSNILLLFDSIEDIFPQLQDSAVNNNRENVTTLQQEFVNIIQEKHPSIFIIGITNRIKSLDSHTRNTFEEEIKMDPPNSAQRLEIFNYYLLNRNSDGSNSNSNNQKVIETINDGCSGLVIGDIAILCRDAKFNATKRLSTSTTTTKENIEFLETDFINNYGQYRTLDKNGGNGLLSKISPSTPKVSWDDIGGLEHVKKVLKEMVVWDYKHHDSIKRLGITTPKGVLLYGPPGTGKTMLAKAVAYEANATFIPINISEVVQGEVGESEKFISETFRIALACSPSIIFIDEIQSIFGHKESSGGNARKLISQLLIEMDATFSHSTDKRVMVLAATNMPQAIDESFLRPGRFDRLLYVGPPEPQERLNILNRIVSSQGIKLAPNVDLEKIAYYAARFTGSDLNALLKKSGLYAIQRDINTPHITQEDIQKVYFESKPSISDNQIAILKDWERSRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVH7</accession>
    <name>A0A8J4PVH7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003901</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000129">
        <property type="protein sequence ID" value="KAF2074798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVH7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550:SF11">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140860">
        <property type="entry name" value="Pseudo ankyrin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="659" mass="76849" checksum="CFD8EAA8028206C1" modified="2022-05-25" version="1">MDRLFKEVFQNAFLRKKIFYQVANDVNCKTRSYKYCFDVYWLLRKKYYSLLQLKLKHNEYLTFNKQAQELIFQLQDTNLFIECYKRLNFSSCGQVDSANSVALLINAARGRNRGNIYYFLEKINYKFSRSMILLQYLVEMNDVPLVREYLKQTKYVEDEIPFYLAIETKNTEMIKLIFDAPRKDYGIKVFEKHRERLLTKAMASGDINIFNMVRNYVDEAASSFFSFKKANVLANDQILLSRVFNTSIRSTNTMKYMIENYGASVISRENMFILVATHGCRESVEYLIDSKILTKETYCEVMAKTLLCYGHFQLHEFLQIKFNLPFVPAPKDFNYFRMLNPTKSLDEVKYIYETLKVFPRFDDLEKSASISLDIFKYIFHLFDPATVVADETLVNSIVTKALTNNHFDIIIFLFQNGITRSVWGRLESLPISQTIYFIQQCFKLCTPSPQHVPHLLETLGKFIQFNSDFKMFKTLFNEIYPFVDDKKLFYGSCLCPAARAGRLKVIKFFFDNGLKPDQKSVFPIVLESARGGSLVVLKYILEKSYQDTQEIMNQMTSDSSILMNAIANDNLDCLEYLIPMFPPNFSIPTYSLASSLGSSGNLFMIKYLYGLSAFSTNTTLFGQVLHECRMNGYFDIVRYLLEKGVQDLECNLRLMNPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVJ3</accession>
    <name>A0A8J4PVJ3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_004707</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000171">
        <property type="protein sequence ID" value="KAF2074000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVJ3"/>
    <dbReference type="OrthoDB" id="19446at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="93"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="37"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2074000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="485" mass="55344" checksum="B6DC86EF8794A013" modified="2022-05-25" version="1">MSLANFVVTFRNRIIIGAVASIFLAFGLFKALKGNKSNSNNDNDTKQSNDSSKSNNNSSTTATNQPTSIVANKPLRITSQGINKTENNNQLIDFDLIQLPHHILFQIFQLISPSQFLRCTQVSKKWKSTMLNKLDKAWIYYSKSHWNFTTIPETDSYFEFFKITFIEEKKRLGFAPEKYIITIMEQSAEFTDPSRWASERIFQNSDWWKLRFLEEMNSTLKQTRLVVNNKKNSNNNILNSNNSNNNNNNNSLITKSNSFDNEESSSSSSSSESHIILGNIYFTSSNSPIGTFRFIDKMDAQTKFRNQKWLAKAYIGEESPYTGDVVGYFNFTLFGLDCPEEVFSKYKPSIAEFLKTLTYQTRGTLTRLDSGYTVQSVLFEDCKLSAVFGKYGMNDGDALLDTDNNSAYIASLIETIQKFLDKKGVGYNSIEDGAKVNYTGQTSHNPIRYSGHLGKEALQWEIGLWIYNTVILNNKKLWGRRLEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVW1</accession>
    <name>A0A8J4PVW1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RNA polymerase I-specific transcription initiation factor RRN3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004832</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRN3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000178">
        <property type="protein sequence ID" value="KAF2073872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVW1"/>
    <dbReference type="OrthoDB" id="16705at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001042">
        <property type="term" value="F:RNA polymerase I core binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001181">
        <property type="term" value="F:RNA polymerase I general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007991">
        <property type="entry name" value="RNA_pol_I_trans_ini_fac_RRN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12790:SF0">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12790">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IA RRN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05327">
        <property type="entry name" value="RRN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="591"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="592"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="619"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="681" mass="77863" checksum="7EABBB4ADA782656" modified="2022-05-25" version="1">MPTMEVHTNQPQKPTLPSYEALWNKAISSSKPNLQELITFDWTQSEQTVNSFVDFLVNLVTYKSIYAYECYNLLIGSFEPYKISIKTGTKVAYEKWELIAMKVHFTFNKILSAIPLSSSILANLITDKFPHRSHPAEVQHFYFKNALFVTRYCPTISQTILSSAISSLIQIDVSISLDEMPDDEDLQFDIENENAAAAAFNSTSTSNLSQEAQEGKINAQKLDILMNLMLDYLDIRFGIAKKGSGNIFYFDNCEQQDLSTQDELFSNLIKIFDVTILPTYKSKYTQYLLFYVSKIYQRSSTFAPLNTNASLLTQAANTSNFLTPSFNKYPSSPKSPNTRFSTSQSPKKHKSFLHIDPVNIATGGKILYAESFIRFLFGKLCNEKVPVMYRHACAAYIGGFVSRASFLPNHIVKLVFRDLLNFAIAYVDKVDNKNNPNCFPDPQAHSLFYSVCQSVYYIFCFRNKIILDFSKDFANDKRDEKQREFWQDLHEAFRKISMSRLNPFKVCLKSVVRQFCSVCIRVGIFTKCTGILKNNKKIPLAKSTTNNSPTASGGSNQFDSFFPFDPYLLFHSSKFFANSYTHWKDIKKQGDEENLEELSDEEDEDSDDEDDLLDKLSSESDDEFSDFQGDSDDDDDSDSESESDSELSNDSDSEVDHLTEEFMSFTPEVRLGLEYHFKHRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW23</accession>
    <name>A0A8J4PW23_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase S28 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_003603</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000115">
        <property type="protein sequence ID" value="KAF2075083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW23"/>
    <dbReference type="OrthoDB" id="1735038at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070008">
        <property type="term" value="F:serine-type exopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.980:FF:000005">
        <property type="entry name" value="Clan SC, family S28, unassigned serine peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.980">
        <property type="entry name" value="Serine carboxypeptidase S28, SKS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008758">
        <property type="entry name" value="Peptidase_S28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042269">
        <property type="entry name" value="Ser_carbopepase_S28_SKS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010">
        <property type="entry name" value="PROTEASE S28 PRO-X CARBOXYPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010:SF11">
        <property type="entry name" value="THYMUS-SPECIFIC SERINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05577">
        <property type="entry name" value="Peptidase_S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035150759" description="Peptidase S28 family protein" evidence="6">
        <location>
            <begin position="23"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="466" mass="51144" checksum="AD57177A65DD7B17" modified="2022-05-25" version="1">MMMNRFLSALLVVCVISNSVQGLAFPKGAASDEVANSNPSNPLIFTQTLNHFDDEDTRTFSQRYFINDAYYNYAKGGPVILYINGEGPVTSAPSSSSDAVVIYAQALGACIVTLEHRYYGGSSPFDDLSTENLKFLNSRQALNDLAVFIVSFTNNLSNAGKVVTIGGSYSGALSAWFRIKYPHLTVGSIASSGVVNSILDFTAFDEWVAYAAGQECANALRAVTKAAESMIFQGGEYEAQVKQLFSAESLTDNGDFFYFLADSLSEGIQYGFHYELCNPLIEAMNSNGDILEVYSNYTHNVWSPMLGIPDEYATSWQQNTTVDFNKADRQWWFQTCSQLGWFQPAPENDPIRSTQVNMTYFQNHCEQVFGVAMWPNVENTNIYYGGNNTAGTNILYINGSQDPWSRASVISTLSPTQQSVTVTCMTCGHCVDLRGCPGGCPSPNNLDQVRQLSIDSIKSWLAAASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW28</accession>
    <name>A0A8J4PW28_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable enoyl-CoA hydratase, mitochondrial</fullName>
            <ecNumber evidence="2">4.2.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_004699</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000171">
        <property type="protein sequence ID" value="KAF2073992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW28"/>
    <dbReference type="OrthoDB" id="2018133at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000019">
        <property type="entry name" value="Enoyl-CoA hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.12.10:FF:000001">
        <property type="entry name" value="Probable enoyl-CoA hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004517">
        <property type="entry name" value="PRK05862.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF54">
        <property type="entry name" value="ENOYL-COA HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00073937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2073992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="281" mass="30706" checksum="623EE41B54C0A930" modified="2022-05-25" version="1">MFSQVLKATSKNSLVFRQYCTANNSGYQYILTEKKGRVGFVTLNRPKALNALSDGLINEINNAVHLYQDDKDIGSIVITGSEKAFAAGADIKEMEKVTLPEAYNRDMLAQWHDLTKIRKPIIAAVNGYALGGGCELAMMCDIIIAGDKAIFGQPEIKLGTIPGCGGTQRLVRAIGKSKAMELILTGNNLSAIDAERAGLVSRVVPAAELLSEATKLAEKIASHSQLTVAMAKEAVNESYELTLQEGIKFERRLFHSTFGTHDQKEGMNAFVEKRNPVWENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWC0</accession>
    <name>A0A8J4PWC0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase S28 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_004582</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000165">
        <property type="protein sequence ID" value="KAF2074097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWC0"/>
    <dbReference type="OrthoDB" id="1735038at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070008">
        <property type="term" value="F:serine-type exopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.980">
        <property type="entry name" value="Serine carboxypeptidase S28, SKS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008758">
        <property type="entry name" value="Peptidase_S28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042269">
        <property type="entry name" value="Ser_carbopepase_S28_SKS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010">
        <property type="entry name" value="PROTEASE S28 PRO-X CARBOXYPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010:SF117">
        <property type="entry name" value="SERINE PROTEASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05577">
        <property type="entry name" value="Peptidase_S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035262628" description="Peptidase S28 family protein" evidence="6">
        <location>
            <begin position="18"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2074097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="479" mass="52739" checksum="E4434A173BAEDB17" modified="2022-05-25" version="1">MKYIILSLFLTILVTNAVRLGHDSALNKGLKYYRGVDKLSASSVSDPSPMWYTQQVDHFDPSNSDTFQQQYYVNSTYYAPGGPIFLVLGGEGPLTAGYVTGHFVVNLYAQKFNALIVAVEHRFYGKSMPKPDLSTENLKFLTTQQALADYATFRQYIAQKYNTGNGKWISFGGSYSGSLSAWMRLKYPHLIDGAIATSAPVLPQLDFPEYFEVVSTSVGPTCSAVIANVTATVTQMLANDRASVQSIFNACDPIVTDLDVATFMESLSGGVAETVQYNNDNNKYTMSNITTMCAQLEQGDPLQAFASFNNRYNTFSGANCTTSSYNSMIQSMQDVDPNGDNAASRSWTWQTCIEYGYFQVGESPNQPFSSTITLDYFLQQCTDIFGPPGYQYQPAIDWIIADYGATDYQGSNVILPNGLVDPWHILGVLQTTNPNIQISIIPEGAHCSDLYPPKPNDPASLIATRQKEVALISSIIYSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWF5</accession>
    <name>A0A8J4PWF5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_004691</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000171">
        <property type="protein sequence ID" value="KAF2073984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWF5"/>
    <dbReference type="OrthoDB" id="272141at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14137">
        <property type="entry name" value="STKc_GSK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001060">
        <property type="entry name" value="CMGC family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050591">
        <property type="entry name" value="GSK-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039192">
        <property type="entry name" value="STKc_GSK3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057">
        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057:SF0">
        <property type="entry name" value="PROTEIN KINASE SHAGGY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="31"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="328"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="328"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2073984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="397" mass="44203" checksum="09F72EAFA806CA16" modified="2022-05-25" version="1">MTIPDNTSSLINDKKEVNMKESSSGVKSYPYDIIRQVGQGTFGKVYEAKNQDSKRVAIKKVEKSNHFISREYDILKVVNHPNCLRILDMFYTAEDNKKMQNLVFDFIPYTLAILLKKRHLTISFIKVLFYQLCQAIKHIHSKNICHRDITPNNILLNPKGELTLADFGSAKILDANHTSMSYICSRYYRAPELLVGCTNYSTKIDIWSIGCILAEMLIGKPLFPGTNSHDQLGRIIEVLGSPTKEDLEAMKPSKPYVLQLPNINPKLFDTLHNVTDKSVIDLLSKIFIFDPIKRASIDEILEHPFLEGVNLSTIELFDEMKCFTTNSSSTTNAASSSSKPSTSSSNTTASLNGSSSNKTTSESFLSRLHTSAITSTNNLKSLSNSSGGVITNPVTTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXG8</accession>
    <name>A0A8J4PXG8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_003615</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000115">
        <property type="protein sequence ID" value="KAF2075095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXG8"/>
    <dbReference type="OrthoDB" id="18952at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd15841">
        <property type="entry name" value="SNARE_Qc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12791">
        <property type="entry name" value="GOLGI SNARE BET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="8">
        <location>
            <begin position="59"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2075095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="151" mass="16557" checksum="FCF25E835E3EAE4C" modified="2022-05-25" version="1">MSNNNNNRPNPASPYLSDNARNALFDNDGIGTSSRKWGGGRDQSAYSNMSNREILDGQKKTMDDQDKHLDSLSHSIGRTKEMAIEIGKTAEDHSRMLDELDVHVDSTSARLSNATRSVISLARETKTTGYWVTICLLFLALIVVSGLAATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYB5</accession>
    <name>A0A8J4PYB5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Vacuolar-sorting protein SNF8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_003918</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the endosomal sorting complex required for transport II (ESCRT-II).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the SNF8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000130">
        <property type="protein sequence ID" value="KAF2074772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYB5"/>
    <dbReference type="OrthoDB" id="283883at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000814">
        <property type="term" value="C:ESCRT II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000085">
        <property type="entry name" value="Vacuolar-sorting protein SNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000397">
        <property type="entry name" value="Vacuolar-sorting protein SNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016689">
        <property type="entry name" value="ESCRT-2_cplx_Snf8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040608">
        <property type="entry name" value="Snf8/Vps36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12806">
        <property type="entry name" value="EAP30 SUBUNIT OF ELL COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12806:SF0">
        <property type="entry name" value="VACUOLAR-SORTING PROTEIN SNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04157">
        <property type="entry name" value="EAP30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017215">
        <property type="entry name" value="ESCRT2_Vps22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR017215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2074772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="244" mass="27899" checksum="23A547F95591ABC8" modified="2022-05-25" version="1">MRRGVGIQAVQKHTLQQKQLQTIGEQINQDSVNKIKDQLVVFKENLEQFAIKHKKDIQKNPEFRKHFNDMCLMIGVDPLASGKGFWSQVLGVGDFYYELGVQIIEVCLKYRSSNGGLIEMNLLLEYIRKMRGKNSIEISCDDIESAIGKLKVLGNGFNILKIDSKKIVQSVPCELNKDHTDVLVVAQQKGFTTVSIIQSELKWNLERTNSVLNFLLQEGIAWIDDQSTEVMYWFPSLWGNSFEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ33</accession>
    <name>A0A8J4PZ33_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_006751</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000325">
        <property type="protein sequence ID" value="KAF2071936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ33"/>
    <dbReference type="OrthoDB" id="21010at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000005">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052235">
        <property type="entry name" value="Nephronectin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24050">
        <property type="entry name" value="PA14 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24050:SF28">
        <property type="entry name" value="UROMODULIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035211967" description="EGF-like domain-containing protein" evidence="8">
        <location>
            <begin position="24"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="998"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="229"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="308"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="351"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="251"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="361"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2071936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1048" mass="117793" checksum="5062813CD1002C4C" modified="2022-05-25" version="1">MKLIHFITIILYICTIIFHRVQSKCPPNFIEDTNGDCTGWSSGIKNIYLSSCYFNNSQTISFILQEPIIKEKFAWNPQQPYTNPLVKNVHFCKSMKVNDYNGTSFDLAYEISMLDNEIAYAICTHKNGVNYTAIRSFSDAVPEDFQFKSLCGNNTIRLQGIKKNIKNTNLETCFYNYVCTNPVSSYQQYFFADCSSKDAEIGGYLATCVANKGHITYTQKIELGMIIRQSGSCSNICVNGLCNSETLSCSCYNGYTGINCDRQALKVACDDENLCPMENSQCFEGTDNNTCACNRGFISITNATQCLDINECSLGTSSCDLKSTICENTPGSFNCKCLDGFTKLNSNQCIDKNECEDPTICSKKDNFECKNTQGSYTCQCLPGYFLNGTTCIDKDACSIEGSSCPIDSKCVILDDNHFYCKCSIQDYILLNNKCIPFPTIENIRTAKSKGELKMDIYINQSIVEDINEIRIDTIPCKEIIQFGDPSLGFLSLSCKFEPGNHVNWTKVVVTINHSSKEFVPTILPAFIESITSAPTTGGDVVLSVITEMDLKDENISLSIGDFNCRGSMIVSSNSIICRVPTGTGIKKVLLNVNNASSTDSILFSYMPPLISHMTKNIDSSLGGWVEIQGSNFGTRPNAIHVYFDLNRKVICDSVANLTNHNSFSCFLPPSNHFKVPVVILVDGVLTKDPFYYYYSSATKVECLNDGINYLGVCQCKQGFNGPKCNISDEDLLFNQGKASFNSNEDPRINFLVENDTMIDTRFGNIGEYSEKELIIEYDLQSMPWEVEMKTESTFSKYIFSSILPNNATVFINILRFNSNFDLQVDSTDQSSQTSLKMKNGSGKMEITIQNWPFSSRLSHLEIEYITDIDKDCVIPSISYAIQNYENQSLVEYDFHYLDQDSPLDNENYQLLWIQYHSGYYTFYTKFINTGILDGEETPVKFLKPTIDETANLVEITGILYVPYFKNSAKIDPEYTLYTSSDPMGNNSLCVDKKGLEKWVFISSITIIIILGIFTIASFSWYLYKRNQPEKVEPFSNLLNSEETTISIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZI7</accession>
    <name>A0A8J4PZI7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Translation factor GUF1 homolog, mitochondrial</fullName>
            <ecNumber evidence="8">3.6.5.n1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Elongation factor 4 homolog</fullName>
            <shortName evidence="8">EF-4</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">GTPase GUF1 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Ribosomal back-translocase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_002848</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.n1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion inner membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the GTP-binding elongation factor family. LepA subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.n1" evidence="8"/>
    <dbReference type="EMBL" id="AJWJ01000083">
        <property type="protein sequence ID" value="KAF2075862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZI7"/>
    <dbReference type="OrthoDB" id="1074at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097177">
        <property type="term" value="F:mitochondrial ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03699">
        <property type="entry name" value="EF4_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16260">
        <property type="entry name" value="EF4_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01890">
        <property type="entry name" value="LepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03709">
        <property type="entry name" value="lepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000078">
        <property type="entry name" value="Elongation factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000015">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000007">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2570:FF:000001">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000004">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2570">
        <property type="entry name" value="Elongation factor 4, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00071">
        <property type="entry name" value="LepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006297">
        <property type="entry name" value="EF-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038363">
        <property type="entry name" value="LepA_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013842">
        <property type="entry name" value="LepA_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035654">
        <property type="entry name" value="LepA_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01393">
        <property type="entry name" value="lepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43512:SF7">
        <property type="entry name" value="TRANSLATION FACTOR GUF1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43512">
        <property type="entry name" value="TRANSLATION FACTOR GUF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06421">
        <property type="entry name" value="LepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="9">
        <location>
            <begin position="52"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="61"/>
            <end position="68"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="144"/>
            <end position="148"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="198"/>
            <end position="201"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2075862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="667" mass="74408" checksum="9CFFAEBDEF2F8A36" modified="2022-05-25" version="1">MLSLLLKATPTLSSSCRIPSNVVSISFKSNDKYRYFCTYTPEKINVSAYPPNKVRNFSIIAHIDHGKTTLSTKLLSYTGTLPKNIYGSGEDSLGLKEKNENRREQYLDKLQVEKERGITVKAQTCSMVYKHKEDGENYLLNLIDTPGHVDFNYEVSRSLMACQGALLVVDACQGVQAQTMANYYLALDSGLEVIPVINKIDLPTANVEMVKQQLKETFGFHPDDCVLVSAKTGLGIPDIFPAVVERVPPPTGDPKKPFKGLLFDSWFDRFRGVITLVNVLDGQVKKGDVIVSASSLQEYEVLDLGVMHPEQEPTGAIYTGQVGYITPGMKTTQEARVGDTFYKKDFPVEALAGFKPAKQMVFAGIYPVDSLDYQSLKEAFEKLMLTDSSVSMSREHSNALGQGFRCGFLGLLHMDVILQRLEQEYNQIVIATPPTVPYKVVLTNGKEKMVSNPAEYPDPEEIANTQEPMIVGHIVTPSEYYGAIVKLCMDSRGIQLNHEHLEGERAKLTFSLPLSEVVTDFFDNLKRISSGYASLDYEDDGYKEADVQKVKVLLNTEEVDSLSAIVHRSRAQRHGRLIVKKLKKIIDRQMFAVAIQAAIGNDVLARESISAMRKDVTAKCYGGDITRRRKLLDKQKEGKKRMKQMGCVELSQEGFLKLMKSGDVDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZW0</accession>
    <name>A0A8J4PZW0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA (guanosine(18)-2'-O)-methyltransferase TARBP1</fullName>
            <ecNumber evidence="8">2.1.1.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">TAR RNA-binding protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_001600</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">S-adenosyl-L-methionine-dependent 2'-O-ribose methyltransferase that catalyzes the formation of 2'-O-methylguanosine at position 18 (Gm18) in a subset of tRNA. Selectively mediates Gm18 methylation of tRNAGln-TTG/CTG and tRNASer-TGA/GCT. Gm18 modification can enhance the stability of modified tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>guanosine(18) in tRNA + S-adenosyl-L-methionine = 2'-O-methylguanosine(18) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20077"/>
            <dbReference type="Rhea" id="RHEA-COMP:10190"/>
            <dbReference type="Rhea" id="RHEA-COMP:10192"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74445"/>
            <dbReference type="EC" id="2.1.1.34"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:20078"/>
        </physiologicalReaction>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.34" evidence="8"/>
    <dbReference type="EMBL" id="AJWJ01000039">
        <property type="protein sequence ID" value="KAF2077091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="241340at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141100">
        <property type="term" value="F:tRNA (guanine(18)-2'-O)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18091">
        <property type="entry name" value="SpoU-like_TRM3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.10:FF:000010">
        <property type="entry name" value="probable methyltransferase TARBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056921">
        <property type="entry name" value="TARBP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045330">
        <property type="entry name" value="TRM3/TARBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044748">
        <property type="entry name" value="Trm3/TARBP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12029:SF11">
        <property type="entry name" value="METHYLTRANSFERASE TARBP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12029">
        <property type="entry name" value="RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25050">
        <property type="entry name" value="TARBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TARBP1" evidence="12">
        <location>
            <begin position="324"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA/rRNA methyltransferase SpoU type" evidence="11">
        <location>
            <begin position="1453"/>
            <end position="1593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00093266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00093594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00093636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00093656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF25050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2077091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1603" mass="185825" checksum="E220CE74E7362F63" modified="2022-05-25" version="1">MTDIFFKQLYTFSSKFKDNSSIFKDEKSIIFESFLDCLISLDIPHVQAQSLLSSTIDQLSEHNNNENVLYVHILLLASKGLLNRLLTISQDQEQVAQVDKKVIENLFLKCYFPLLVVENDNDDFIFKKVVEIVTGFLLPIYRFDSNSFSCVVDKFIELSSRLYNTILDESKELLLNTNNNSNNSSSNNNIKNNNNAKIIILLKKFFENVNNSSNLEIQSIKEFRLKIFKDSIHLLLSFGLIDVRNTIMTELIRPLIENQDELFKELNEIIYKMILNDMGVTVSIDGYKLLSLFVDQNYFNSFVFDSRFWESLIKGLKDIEPLNKKRSSYILKKVIKICIEQENELDKYQDQWQKYFKWSSREGLQLQKLWNTFFLIYESLDEFTLHLISPVWKELDSLIILNSSMTKLDFHWIDILFDRAMTHQNPAVIKALVIEILNSKQYTKYLPLEFVTGTLIQSVSNPILYKGVTEATIHTSVQFFFNNYFDSLDCVEKKSTLYNSLFTSMVENHFYKDFLTNLVKFIQDSCQRTKENIPLVNDKFFSTLYNLLEIAVKRLHNTGRLRIYKYLIKILARITIFDQFSYQKVSKLFSTIPLSLHTNVKYHQLLKQYLNCFNEKDWLFNNIKKDLDNSSDDNDQGVIVDKDLIIKFKTESFIKTRMVCYLNQDQYNLIIEFILNSCKSSFISNSNNSNSDYKKIINLASILSISHLPYHNLDSIISISEFSKIFINYLTSTMNQPISKDPIVLLELEESLFLILSNMVKYLDNSEIHRYLNLVFKQIDSVLTFDQTDLSQIQKVVLVQRLFGIFRERSNIKTTNFTIQQVEKVLEYLFRLELVKKSNQDKSINDHWGSINSYFIKTKWMLGRNLFDFILSNISNIKESSLLLTKSREYLDVLLEELSGSNFYCSLPVFHCLSMIFPIGARVDISDENSAFDQEIFEKVLYSAWTTARDPGTLTYMGCFVQLAYHPQVVSKQVDQDSYNHHECLARYLKKTSEQSQYTFGLGNLLALKITSIWKEYPEIANLYVEEIYDFIVNGPLPREDSDLALESQGAMHMYEPLIGIPSHVFNLHQDSACETSQDEEKFGFGTAPFKESFGRAVFAVFIKNIAQLAQDKESPKHSLYNNLLNDIGFKFVEMNFDAEFTKKKEYILNTLPHRKKIRIWQTLCTISPYINPDEGKLNQFADQLIKIFYLFNLPSVRRYVNIFIVNFMKRYHQIIDSHLLQLVDNVNLRGDILSSIVVTAGNVLLNIENNDQLFEKVFCKILALGCSGHMIVKVVACSMISQLIDNMSDLKNRLSPGLMVYLAHLDFFFKNNAQAAKSLEKQKGQNGTESDKELISPEFENIFYNIPVLEILSPTEIINPGIFDFLLNDYNSLLIDKELKNNLIKKSKNNVKQDNVDEKEEEGQQEKINSIQDVDEYIENNPTSYQKKILPWAELDLESRDSLGKKVKQDVIICASFVDKVPNLAGLVRTSEIFNISTLVIPDIKVMSDPAFLQITVASEKWLNVEEVNPTNLKDYLLAKKKEGYSLLGVEQTSTSKQLNQYTFPQKSLLLLGHEKEGIPAEYINLLDQCIEIPQMGITRSLNVHVSGSILIWEYTKQQLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q058</accession>
    <name>A0A8J4Q058_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_002013</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000052">
        <property type="protein sequence ID" value="KAF2076700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q058"/>
    <dbReference type="OrthoDB" id="10261837at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037370">
        <property type="entry name" value="Pleckstrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12092:SF16">
        <property type="entry name" value="PH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12092">
        <property type="entry name" value="PLECKSTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="648"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="105"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="168"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="242"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="378"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="457"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="65"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="276"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="105"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="171"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="242"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="467"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="500"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="553"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2076700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="753" mass="82341" checksum="E6C986A3EA11E532" modified="2022-05-25" version="1">METDINQITHQQEMSVDNNNNNSIDRENSSSKNSNSETVVVATLDAAPATAAVDDNSNGNGNSKIADLESKIKELSLQLESERTKQNQIESENLNLKKKLEQLSPNKTTPMSATPSPLSPALSSSSGNSSSGNNSNGSSSSINSKKLEETISKFEEQVMNLKLCLTKRTPSHSTPASPKLTMQPTTPLLSPSMQSATLTQSPSLTASSVLSPMEQQRILQSKLDSFEQKYSSIKNILLDISSGEHEQDHKSSSSPLSPSTTTAAATETTTMSDANIEEMKDKIDNIKEVVEDLKKEINDQDCIIKQQEEVKEQVEEEVKQEEQIKPLDKNESKEAVSNISVDINNNTNNIDNSNIVITDIDELNNVNQQQQQIVNNISTQDDSQQQIESSNDNPNQNEKTTLPPTATIDTQQQMNPDEVSEINQQQNVSPPKALMVDIFSDEKKEAPVKYSIALEDLSSLPDPPSTLPTFIVNTNTTNNSKSGKSSKKLNVNPGQSPVGSPTITPKSTSTTNEDSNNTIPTPISNTNNTTTTTTTTTNTTTNTTESQQTDLENSNSSSSVSTTNSINNTNNNSPTPTTTLTTPTPTGIVNETNVNNDNNSSNSNNNNNNSGEDNNNEQDNNRFSTMSSTSSSSIIEEYSNVKVVALTDIVKEGNLKKKGGGEGGRRNWTTRWFKLKNDSISYYKKRKDARPKGIIKLSHAKVETHSGKPFGIVILSGINSKNIMRDYLLMAETKKEQDEWIQSISEIINKLQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q063</accession>
    <name>A0A8J4Q063_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome assembly protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_006360</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for efficient biogenesis of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RSA3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000290">
        <property type="protein sequence ID" value="KAF2072326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q063"/>
    <dbReference type="OrthoDB" id="69550at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051898">
        <property type="entry name" value="Ribosome_Assembly_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028217">
        <property type="entry name" value="Rsa3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28127">
        <property type="entry name" value="RIBOSOME ASSEMBLY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28127:SF1">
        <property type="entry name" value="RIBOSOME ASSEMBLY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14615">
        <property type="entry name" value="Rsa3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Ribosome-assembly protein 3 C-terminal" evidence="9">
        <location>
            <begin position="319"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="9"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="85"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="150"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2072326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="376" mass="41878" checksum="304E53C2F56A2874" modified="2022-05-25" version="1">MGKSDSESENSSMSESENSTSSSSESESEKTIKRKSTFTNDSKKKKKILKKNSSSDSESDSSSDSESSSSSSDSESSQDEKIVKKNSSSESESGSSSDSESDNSDKESKKNVKETKEKESSDESDSSSSSSDSESSDESRNSSSSSSSDSDSDSDSDDNDKDSEKDKSDSESNSSDSDNESNKDQEFKLNENIDQLLDTLHGKNGNGLVSTLSFPNKKRMAKKTQEEKDYFIDKASILSTRLLLLLRNTLSLQTQDKSSLTHLLHQLSFQSSNYNDLSIWKDIQKINLNILNQVDQSISLDKNEKQTTTTTNSNVNKNFREFFIDKMTNEFGDDLDKLRDDPFYRDSMLTMILEGMESGIDLYSLQDKQLVLKNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0C7</accession>
    <name>A0A8J4Q0C7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Armadillo-like helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_006317</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000286">
        <property type="protein sequence ID" value="KAF2072371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0C7"/>
    <dbReference type="OrthoDB" id="2012278at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039868">
        <property type="entry name" value="ARMD3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013636">
        <property type="entry name" value="ARMH3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13608">
        <property type="entry name" value="ARMADILLO-LIKE HELICAL DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13608:SF3">
        <property type="entry name" value="ARMADILLO-LIKE HELICAL DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08427">
        <property type="entry name" value="ARMH3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01158">
        <property type="entry name" value="DUF1741"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Armadillo-like helical" evidence="6">
        <location>
            <begin position="611"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="12"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="35"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2072371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="853" mass="97927" checksum="B228815C475C4759" modified="2022-05-25" version="1">MQHQSQAVHSFNISISSKSPPYNSSSTLPPPQSHPSPTISNSSSSSSESPGLNPYKNQSGYYLPISTTDKQEKQQQHQHKYNNNSNSFTNTVDTLNSLAATNSFAIECPKHKQEVDLRDYIKKNSNSDIYSTFLNLDYYNRYFNQNQKQQTQPSILSPSSISSPTSPSLDNNNNNSFCLCDLKTMLLSRSDFIQDIDTDSNLLQEYHRLKMLQEQQGPKHTEILLRKEDNVGGRKALKEKFVEIFEAFFNDEDPSSGDPKFWEQLFLLKVNIPFLERCIILSSEDRLLVLKSKLNNIFTQSCFWLKNSDPTRLSHIIEILSIMLKSIFRKKFNNFGFDVINILCGIDKADTVFPEFIKDSTQLLKTSDVNIKIGIINLFNIIATATDYMNQNTLLQFFMTVDISRTLLELLGDKELPKSSKMNIMTLLCYLSNYQKYETQNPYIKSLSNLDDIEKIKQLLDVITEGIKDHNKLFEEQFRQPNLGMAAKVGGYFSSWIYTPPVVHNCSFFETGSCLLILYELFYQNETFVKQLVGGGWSREKSCPEILSQFFTFCSYLASDTPKESKALYSKISLLILMLISESRELQDYFHDPKSTSYIFIYSKKSLFPDPKELEKHPLSFHVVDILSQFIKCNLKGKQSMDTHQMSVDCIQRVLSYKKKTQSRLPHKWADLWSSLFALLQVLPTIQDKSFLNQSIQVGVATVNIFNLFINYGDSFLPTPNDYDDLFYEIIRSGNVVDNFYIFIQQQPQNDPTGALLNSLLNIKSIVQHFSGKLQEWSSANPEVALTAPQVSKIIKDNYDTLRLKLQENLDQYNSYVENKEIALFRHYVKELVCDLKKQIVVTPNAPPSPPMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0G2</accession>
    <name>A0A8J4Q0G2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002929</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="EMBL" id="AJWJ01000087">
        <property type="protein sequence ID" value="KAF2075747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="20544at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19673">
        <property type="entry name" value="UBR-box_UBR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055194">
        <property type="entry name" value="UBR1-like_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF37">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22960">
        <property type="entry name" value="WHD_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBR-type" evidence="6">
        <location>
            <begin position="72"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="4">
        <location>
            <begin position="72"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1523" mass="178144" checksum="1653B05BA2CFEB9C" modified="2022-05-25" version="1">MITSLNNHPKPIQVVPVLTSMHDAPFNYKYCLESYLEVKLKSLKERDIDNFVASLSGETILTLIEKTFIKLPFCQQEWTANSYFFKCNDCSIEKNSSVCVNCFKKSDHLLKCKSFSLEKSLSGGCCDCGNSDCVIEESFCKDHSVSEDPSSQLDEKTKKSLHVTIRLLVLFILRCIDELGATPVNDRDHIQVLETIKNIFQWFEKVSKQSYSILYIIAQEFSGRSLKENSFEIYKPIDLPFLDMVPYNFNENAIYKLLLESKKFPQIIDYFMPLAFTFFGNKIFKIIFLEELLKFFKDFSLDQKLSSQKRSLVEQLIPLQSISFPFINGQSKQNIFIVIFNTYTTIFKKVAMINLKIPSQRSSLNLILDDIVEFRFFPELFKKEQIVNQIYENDEIIRSFLQTVNEIQFMDTFTRSIDKADENTLPSLLGILSIEGEIKLAFGNLFKTLHKYKFNTTEKYFDLIVRGFQISSPNQGYHFCDGLELPNCNILKGIEPLSLYTLRYGIVHVFFSKIVFGSPDFSFNYLNSNFDRKTMILLTSKAIIRSVYVSQYESNLWKRNGVDIYAQYIWLNLELELFFIQLMTILLGPKTIITLCLSSFSSNFQHDPQYKNMLPHFLRFLIFVLQYRHHIQIDSDSQKYHLIQHLASGPKTYTQLKDCRPEYWEGISEDSIDLVAHFKESDKKYHLKPTMWERYDPYYPFYYEKESKSKSMENFWEHLKQKNHISQESYPLPCKIDTLHDNMKQVLSLLQEPLLYEMFSCNISSLISLPTVKGKTVKYRINSDSELIKFVQNEIDYQVNCLESENNEPYYDFQSGNQYLDSLLNHSLYTIVMALKLFIETNFKTMDKQTISKIHRCLDFYFKDTNRNTGKKQEFDSFLSEMNVSYTMNPILCLLKPFTFIVDDGVENDKSEFVVFNLIDLVIMMSVKMENENHCKDKKDLFHQFYSFLNQINNTTLENHLLSSINLKSFLSDKDLLEAQERKKKALQKQTEIKEKIRLQQQSISLDKYLSYEEESFDPISPDNNTDGQVCVVCKLHSPVPLCEIGYVSNSAVKRLSIRETKSQFKDPGHYLEAVGDELLFYQNTWHDFNLYSIFYIIQFGCSTFAQCCGHVIHKSCLEQLHPSVLKQNECFYCPMCKRVSNFILSTKVEKMDSDFANSLAMTFLLHDLTNVDPPPKNKDIYKCLLWKYIISNIENLELLTRPNHNYLEGLEKQSSFGDYQNPMIFSETEFKRHLKTLHLIYVNIMAITSKIDPTFFELDKNMDFFTSRDPFVLLCIRHFLYPHKDLMTETLPACFKLMVSDVRSFQYGSTQKNISYSHPLKGKESIDSYLKPFIRKAFLFIHYCIKSQESIGLEFFNNIDLLKTKLGLSSPLSLDLKFIPIPFERLFLNNFFITHLPTFTYLPSKHIELALNNLNKPLPTKKCRDNDKGFCLFCSTLVCLNDECCPQQNLIHSFGCALQSVTVKVCTPQTNVIKETPYKHQYIYDVYFDKHKQAGGLRSTSDFRLNESILRRLYVDWLKNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0Y2</accession>
    <name>A0A8J4Q0Y2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000049">
        <property type="protein sequence ID" value="KAF2076774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0Y2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14596">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14596:SF72">
        <property type="entry name" value="ZINC FINGER PROTEIN MSN2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="429"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="609"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="429"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="451"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="609"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="644"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="703" mass="82196" checksum="94D74B4BA2AAC771" modified="2022-05-25" version="1">MEEDIEEISSNNNQQPPSSNEDDDNNEEDDKQQQRLKGHKDNFKKSLDILYRAYQAIFHFIRDEKPSIVFHSPNSTRKNSNTYKYRMVYFDSNALPKQAENFVRHPNDPDAVISFTYRFELVHTKTLFKNSFVQFLLDMIKSRYKSYVLNDKRILKSPLNNLFSIDQFADTLYQSFMQYNPAQILNSNDCRIISTALCNHYERKEYKRHPTSYKVKSFKKKDIIEKILVSKGLDYIVEEDSKTQIHNLVNINSIKSNMLLSGDSISSYLEIPDKENAKQVLGWDNEDDRIGRPDIIPDIFLLDYLLAKLLSNVGTILTENTYTSTKNPFLIYSFQTTFDNRPFQIPTDSKSKLIVLPLSMKGHYSFALFSIDSENCIDIYHIDPHVGADCPLCRKKSFLFHFVKKNFFHVENIRYNHIYTQQQTEFEENTNINNNPNHNHNSIPDDDDEYNNSNNSNSNNNNNNNNNNHNSTSDNRGFFTILNIVAMVQDLNQQWIDNCNFNISQLFEFINNQRLALVEGQPNTLFNFKIVEINRLEFKHLLKLDYIDVIKKTKKILLTNQPIPTEIHESILRISRYLNQNFSTTESTSIVNNNYNLPFNSLLNLFNNQNQINSNNDNSNDNNNNEDDQENVNNQEQNQDDNMDSNNNIDNNDNNIDNNNNIDDDNNEKRKDLEINIDDNDSKGNLLKKQKTINQYVKSSPDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q194</accession>
    <name>A0A8J4Q194_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="7">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_001777</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="7"/>
    <dbReference type="EMBL" id="AJWJ01000044">
        <property type="protein sequence ID" value="KAF2076939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q194"/>
    <dbReference type="OrthoDB" id="29137at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2230.10">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035927">
        <property type="entry name" value="DUSP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006615">
        <property type="entry name" value="Pept_C19_DUSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028135">
        <property type="entry name" value="Ub_USP-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF24">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06337">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14836">
        <property type="entry name" value="Ubiquitin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00695">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143791">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51283">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="DUSP" evidence="10">
        <location>
            <begin position="83"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="297"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="632"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="969"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="58"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="638"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="650"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="667"/>
            <end position="676"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2076939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="996" mass="112534" checksum="718EBED21E7959B0" modified="2022-05-25" version="1">MTESPGLNNTATDSTSVGSFECISSSSSSFSGKTTAEGQFDSPMTDAAQPSSEQDNSNNTTTPINEDNENNNNKDNEQQPQQQLAKEERDVLVETQKTTQLEEGQKWAILDNGWWAKFAEGEPVGPIDNSLLLIGNNIKPGLSEGNHFTTVPDTVWKKLVQVYGGGPEIMRSVVKNYTNRYQIDLKIPFMLQFMKSSSLQTIVQIPAYKHETISSLKERACKEFQLKPEDVRIWDYYNQNKYSELKDREYVSNSNFIENQHVLLEERLADGSWPAVKQNNHQQFQNTRTGSARPGITGLNNLGNTCFMNSALQCLSNTPPLTNYFLNNTYVQDLNKTNPLGCNGDLATDYASLIKDIWAGTLNSVAPRDFKSQIERFAPQFAGYHQHDSQELLAFLLDGLHEDLNKVLKKPFIEGKDYDGRPDDVIAKEQWQMHKARNDSIIIDWFQAQLKSKLVCPVCSKISITFDPFMYLSLPLPSQTQKQFNVVFINKDSKIKATTHEVCLIAQTSTGKDILEKISEQVSIPTKNLVLCTFSHMSSSFKIVDPKQGLDNINNRDSVIVYEVETDDEQNPLVVYQQVVVNMKQQDSYYSSRSPFILSVPSTVKTTGDLYKVILDRLDAYLNRAQIDEIFNSHSVEQEEEEEQEDNENDGEKPEKQEHDSEKAVETNASASNADSVSEDDTTTPMIGPLAPSHMSSGSGYFHSSNGYNHSSYASSRSGMDYNSYGYNSNNRNVIPADKKDPRFVFQILDSTKNLMTSIPTTKIGEITLILDNGNMIDTDPLNQAFSRTHPHREVPVEREVSLKRCIELFITEEQLGPDDPWYCSTCKEHQRAFKKFDIWSAPPILVVHLKRFSYKKYNREKLDTLVKFPIEDLDLSEFVLDKSSPKPVYDLFAVSNHYGSMGGGHYTAYAKNHTENKWFKFDDSHVSEVHDPNNVETEAAYVLFYRRRDTAAPATTAEDVTMDLKVNSESSLENDDSNNNLNNTTTTTTTTTTNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1T0</accession>
    <name>A0A8J4Q1T0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">S-adenosyl-L-methionine-dependent methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_000436</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0677 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000008">
        <property type="protein sequence ID" value="KAF2078246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1T0"/>
    <dbReference type="OrthoDB" id="18859at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007213">
        <property type="entry name" value="Ppm1/Ppm2/Tcmp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011610">
        <property type="entry name" value="SAM_mthyl_Trfase_ML2640-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00027">
        <property type="entry name" value="mthyl_TIGR00027"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43619">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASE YKTD-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43619:SF2">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04072">
        <property type="entry name" value="LCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2078246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="392" mass="44840" checksum="6912D62426F8660A" modified="2022-05-25" version="1">MIENSENSNIQQVLQSVGKTSVWISAIRAVLTAHFIDSFGKGNDQEGGQAVVSGGGAGASNINLSINKTKEFELYAIEIIKKKFGLSNFKDPRMEIKYPVDLVFDPYSFYFISKLNTKELILSTYDQYFNFTYDKQFDQLSLEMKSISKTELKRKEMDRIETNFHSVLSWADLMSSHVFYSIAKRTHLIDKVFLDSYQEGLQQFVVFGAGMDTRSYRLPLDAKCTIFEIDLPGVLKFKEKALQEAIKEIPPVGQCKIQFIPSDLSSLDWNHKLKDMGFDNSKPTLWVAEGLFMYLSESTIESIGELVSANSAPKSKYFVHSLMNRTSTTPYYQPTPHDFFKMKADTVKEEFKYILNLNCKQYLIRHGFSFNVKEIQEMGNNIISIYSMGSNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q251</accession>
    <name>A0A8J4Q251_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000172</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000003">
        <property type="protein sequence ID" value="KAF2078548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q251"/>
    <dbReference type="OrthoDB" id="20940at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.980.20:FF:000015">
        <property type="entry name" value="Histidine kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.980.20">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="421"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="239"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="362"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="426"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="498" mass="55766" checksum="1942AC7D3F1A7673" modified="2022-05-25" version="1">MNHLKSILVEEDSIEQKIIKQQSMDNMSHFMLDKNQNDLKYKPQGPVGASPQGFYFNFKANPNNDSFQNVCQHKSQCYICTRETPPCLSSRSVSWVSILRVVFFSLKKLYPEKEFFNLKKDVYGYVAAHWSLICSKKKKTAGWRKQLQDALSHCRRLFESGADHHECYGFWKLKDLSDPWKESYPNEQLMINRSNNMNINNQSNNMNNNNSNPSSPISSVPQMFTSPLLTPDFSSKTNSPSYEYSPTSSPSKESPLIGNSCINFQSFSPANNSTNSIHLVPISPMPTPPFNFLQSPIANSSSSTSPSFINTPKTLMGNYGNSILDLSNMGISSPVIMHSRPRSKSLSSSPFVLDSPNKFRRSNNGSVNSNSHSTHLTSFNFLINNNNNNNNNNSSNINNSNSPASFRSSCPRLPRLVEDYSEEKSISSSSSSSSSSSSTNLVSTPQISSSIEKLSISNLVNNNNNDSIYINSNNSNNNDIHQVSFKFSRKRSIDLIFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2F3</accession>
    <name>A0A8J4Q2F3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000045">
        <property type="protein sequence ID" value="KAF2076902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2F3"/>
    <dbReference type="OrthoDB" id="21358at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="350"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="412"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="463"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="117"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="417"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="463"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="687" mass="75491" checksum="6EFD9BED763112C1" modified="2022-05-25" version="1">MKISLIQCSSFQETILEEPVVFASKIVYRQQLVEKSAAVVFYKYSSTNHLIYTQDRPILEKMTVVEQDSSKTPPLFVTCSEFKSNQWRPTLCIYCFLPKSKHFVAVDSNSKITATATTTTTTTSSTSTTTSSALGPLSPSFTSSPSSPSSSCSSSQQPVTKTTLFTFQSSTISTTTTSTLPSPKLEPLSTPNNLPKPLLRKKSKSLPHAFIPKISKAVVEKEVLVPSTETIIIDNSDSYSSGGSDDEITPCTFTVSKLDSPSILKPTLVEKECNEKEKEINNNNNTITSSVSITISTSKSSSSLISSPYTYSAKSNSIETSSPSMKPLSLIPPLPLLSSSSPSIIEDEITQDKQDIEEEKNSKTTTTTTTSKRNSIQPLRLKSFSFSASSSIPDNAAFEDDPFLDFEDLVDDDEEQSQTTTFSISTQTPSQTTTFTSTTTILHSPRLIEKTTTTNISSSITTTSNNIVSTTPTPTPLTLNSSPSTNSRSINNNSQSSPTIVGVNKLDLTQPQPQPQPQGKPLSSIIVPSKRRSIKMDQFKEKEDDWDQGMDLTSFLKKKNSIQRDYSYCSNKFNEISSVKDEFQRQSIDFGGSLHPNAFEAFKGILDAKQKQIKKAFERKKIDEDDCLMLIDELNTAKELLQDLLISDENELNINNDSDNNLVGSTTPNQINNNLSQQSICIQSDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2I0</accession>
    <name>A0A8J4Q2I0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001031</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000023">
        <property type="protein sequence ID" value="KAF2077644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2I0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035223398" evidence="2">
        <location>
            <begin position="23"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="289" mass="31434" checksum="E0883BC2DCF0DC67" modified="2022-05-25" version="1">MLKINFFATILLITLSIGSIQGDLYPLSADWRSCSFSSPDGKYMVDLSEFFTWEPQTFYAEYNGRDRVVFAISICGFVSCLGNDVMACRDFTPIATFANREANWYTGTSSNSPSLVITSTAPDGQPFKITIEPALDNVSVITRQSPNSNIFKLYAAPYLWKYSPITTSLASFSSPSIISIFGDLSFLPTDVTLGQVRIFHPDGTSTQCQYPTFNIAHNVINCLNTPQSSDTIYDKNTIIASFVKSNIYSYEYWALLRYTNSPTSSTSGSGSGSGSGSGSGSGSGFGSFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q364</accession>
    <name>A0A8J4Q364_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal protein S15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_000728</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000014">
        <property type="protein sequence ID" value="KAF2078004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q364"/>
    <dbReference type="OrthoDB" id="441444at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_B">
        <property type="entry name" value="Ribosomal_uS15_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_uS15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005290">
        <property type="entry name" value="Ribosomal_uS15_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00952">
        <property type="entry name" value="S15_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23321">
        <property type="entry name" value="RIBOSOMAL PROTEIN S15, BACTERIAL AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23321:SF26">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US15M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00362">
        <property type="entry name" value="RIBOSOMAL_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2078004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="580" mass="67947" checksum="812020ABEA075D73" modified="2022-05-25" version="1">MLRLFQRSLIVSKDLSRSTSNTAFKYYTTTNNDSKVNKREFNKYKAEKRLMNSLFEEEDITKLPSTNQKIDTEFISEQLGLGEKKNALNINDFLSGDIKNKPGYENFDMTTEESRYALEQLEQILRAPNGRLVVQNLIADLKQTFGLETDSSMEAKLKELNKDMSLKLGSDIEAFLKQHADNISALDNKQKDIVKEYYEKHPYLRHIRQTTFILDLVALSDQYKLSDGDTFEENVKKRFSIRSTPENEGVDQFAQQKPLEEINYIRDGSDNQFVTNTTQDVFEDNIIEVIRSGALSLYFQNLVDNAKEAALPASYQLEQLKQQQLLANELDIADRMTLRDQKELDQQEEQDQDQQEDPEKYLEADVIPELDLMLPVEQQQQAKDPRDLLKYYHIYPRQIRKWIDYSHTPLGLFTSYGVWYNLPEWYSKHFQPTPPENFINPQGNSNIFKQTELPEDLQNVYRFGVTEEEAKLLHPKLKEFLSFRNASQPEVNSYRKQLCIQKYGKNSFDTGSPSVQIAVFTERINTIIAHLAKNKKDFPTKRKLGMLESKRRNMIEYLKKKDVEEYYKVTKDLKIKNYNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q373</accession>
    <name>A0A8J4Q373_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000657</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000013">
        <property type="protein sequence ID" value="KAF2078019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q373"/>
    <dbReference type="OrthoDB" id="2364174at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2078019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="277" mass="31384" checksum="78D48C2862CFA71C" modified="2022-05-25" version="1">MPLPLVARKSLRDNQEHLDAAVEKIKAALGVEWTFEIDFETIMAKVTDSDYVKNSPGECFYKEVAGYVAECIDRAAKNEMVKEALIEANTNSKIIVALNEDKKNSSYWTYKFNNGTLELLFKSICNTSDICYFKLENIIPTPGVYSLPARMNLKAAEEKFNEAFEKIKVATGADWSLDEASLEALYPSIDKSYQESIGDIFSEIVEYAAQNITKRLGDEMVKEAFLELTPNSKLVFKCNPKLGSYWEYAFQDGNLVISFRSICNTSDVAYYDFEKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3Y4</accession>
    <name>A0A8J4Q3Y4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_000893</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000019">
        <property type="protein sequence ID" value="KAF2077772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3Y4"/>
    <dbReference type="OrthoDB" id="10056939at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="6">
        <location>
            <begin position="497"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="499"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="185"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="293"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="522"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="18"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="185"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="221"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="232"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="304"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="321"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="334"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="350"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="522"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="536"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="570"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="591"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2077772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="600" mass="68182" checksum="CA8725ABF7397F9B" modified="2022-05-25" version="1">MTTTTNHPELENNTHSCNLNNIENNDNNDNNNQHTDSEQQQENHLYSEHNQQLNNNNNNNSQQEQQQPSLTLEYTQQLESPNTLTQECYNTNDDNHNINNHNSNIINNTNFNNDNGYTNTQQMETNHTDFIYAPYQMDANNIPCYQVFPQYFVHPTYTYPPPFTHALQNHTNNYYNHYQPIQQIPQQPHNQPTISVSSPSSPFTSMSSPTNSPAGDGYCNSSTTSATTPPTFIQQQESLSTPKTLPSPHVFEGSIPSPINAPNKRQTMFLDQNYNSPFVSPSYSVNAPENIFQNQNIQGPCPNKTTTTTTTTTSSTSTPSKSKHKSTKLKNKSQPQQRTASTLKPNQSLPINQNTPSSPSPSTNSTNNLNNINNNNCNQFTNLSNMLSQKERNSIEYCWYESILNSMIIDDNHLKVEGNYSFLEGYLLFKKEFKEVFGEQLSTETMDCISLNIEIQNEISQLLKFHFDSIDTFYTNYVHIINGYSLIPTKEPTSLLKARPKKGTKLSPQAKEILDSWLKSHLEHPYPSPEEKENLQKSTGLSPNQISNWFINTRRRKIPQLTKTDPPPNQSQQSQQSSPSTETITIIAANDTDDEDDQED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q7I5</accession>
    <name>A0A8J4Q7I5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FAD-binding FR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_002787</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000081">
        <property type="protein sequence ID" value="KAF2075896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q7I5"/>
    <dbReference type="OrthoDB" id="1856718at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030586">
        <property type="term" value="F:[methionine synthase] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050667">
        <property type="term" value="P:homocysteine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.990.10:FF:000007">
        <property type="entry name" value="Methionine synthase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019180">
        <property type="entry name" value="Oxidoreductase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF84">
        <property type="entry name" value="METHIONINE SYNTHASE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09791">
        <property type="entry name" value="Oxidored-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="7">
        <location>
            <begin position="135"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2075896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="569" mass="66116" checksum="DAF4D407ECCDAE78" modified="2022-05-25" version="1">MDNSSSNSNSSKDELEDIVLEELPPPPAYPADDECCGSGCIRCVYDLYDMQLERYETLCKKIEERNKLLIEKIKNRNNSSANNDNDNDSNNNNNSTSGINSNFDLKIKITYNTDINQSQQQQQHSYLSSKIFKEMGIRFAKINSFQQLTSNESPNTVFHLDIEPTSSDEIRYKPGDYALILSPNDSDLVNKLLKRLDLLKNKDEKVLLTTCSDDDSNNNKLPEYLPIEACSIKDIFTWNLDISFIPPQFFLNIMASYCTDSEEKKKLVYLSSKQGTVLYNETVVQHHINFVNLLIKMKSCKIPLEILLSFIPSHQPRKYSISSSQTMNINNNNNHSHNNNNNIHITFHLLKKDKLKSTTKEKDKYGLCTYWMFDQIEKFKVNQQNTLFHFSIEPTKHFTFPDDLSIPIILISTGTGLAPFRSYLYHRRYLLSKNSNNNNNSKCLLFFGCRNQDWDQLYKQEINEFVQESIITQLFVSYSRDIKNDDKKKYITYYILENGDEIFTMLQHSNAILYLCGSLEKIGKSIDDCLLQIIIQYHKSNGNKTIPKQQAQLILDQWKLDKKIRKDIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q826</accession>
    <name>A0A8J4Q826_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_002311</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Rho GTPase-activating protein involved in the signal transduction pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000062">
        <property type="protein sequence ID" value="KAF2076406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q826"/>
    <dbReference type="OrthoDB" id="185175at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050729">
        <property type="entry name" value="Rho-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176:SF129">
        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN AT 16F, ISOFORM E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176">
        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="7">
        <location>
            <begin position="19"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="490"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="239"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="451"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="493"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="524"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2076406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="598" mass="68213" checksum="614BB0DD74453DD6" modified="2022-05-25" version="1">MEPISPTASSSKKNNAVTKTFKKILKPGEKKKKQKATTLIAVKYLIQYLEQHCLELEGICRISGNTIKVKELKKQLESEIHSGSELSHDLSKYDKHVVSGTLKMFLRDYDEPLLTFDLYKNFLASADIKEKGAKMAFIKSLLSALPKDHSDLVQVVLKFLYTLQLHSQKNKMTCSNLAIVFAPTLLRLKEETLESMMTDSNIISEISRFLIEEFNPLYEMNTTILYKLKSQDLGIKEEKKSVSEQLEDAKLTIDQLTKQLAEEARERSILETYSNSLDQKLQEQSECLKSVQEEKDSLSELLNEFRETNRKQSEEIQLLTDSKNKIETEKNQLQSLKEQLEKQNTQLNQDLQFIKQEHDKQGKELESIKKLYQTKLLDFEKLSKYSSEQSSQIATLTKDVGVKTDENNAFVQKVNLLTKERDDAILTSERVQGQITLLLNTNTNGKKGSLSKSVKKDYENAQKKLKDYEAKISLLEKEKYQLTDQLSKLKPATPTTTTTTTTTSSSKFIIGKKPNQKDKKGSPAPTPSTPPPPIDEDDLISVKKFAFYWMNLALKTDALVKGENCNIPGSEILEDLLQKNVRVALWNDYVLTKIKELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPE2</accession>
    <name>A0A8J4UPE2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Acetyl-coenzyme A transporter 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_009578</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000747">
        <property type="protein sequence ID" value="KAF2069106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UPE2"/>
    <dbReference type="OrthoDB" id="6415790at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008521">
        <property type="term" value="F:acetyl-CoA transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035348">
        <property type="term" value="P:acetyl-CoA transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000289">
        <property type="entry name" value="Acetyl-coenzyme A transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024371">
        <property type="entry name" value="AcetylCoA_trans_1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004752">
        <property type="entry name" value="AmpG_permease/AT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778:SF9">
        <property type="entry name" value="ACETYL-COENZYME A TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 33 ACETYL-COA TRANSPORTER -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13000">
        <property type="entry name" value="Acatn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="17"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="194"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="315"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="348"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="412"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="486"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2069106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="517" mass="59692" checksum="D3AA068876A60AFF" modified="2022-05-25" version="1">MFYNYLKKTFGHDLHNILWLIALYLIQGIPIGLSFGTIPFLLHKHATYTQIGIFYFSTYPYSLKLLWSPLVDSYYWKSFGRRKSWIVPIQIIAGLFFIAISFKIEYLVEHVQSFILPVTAFFFILIFLMATQDIAVDAWALTILSKSNLHYASICQTIGLNTGYFLSYTIFLALNSPSFANRYFRSTPSEEGMITLSGYVFFWGCVFVMFSIYLALFKGEENNHIIMKEKVDRVEENVYNYSSSSSPITTPPNELNHEIDLEPIQVYKQLWKIIKMPHIKTLALMFLMSKVVFQANESVLNLKLLEKGLKREDIAFFSIIQFPCVILFSILSSNWFKHKPLTTWTYSYIVAVLFVFLNMLGVVNFPQNQEIGYYFLVLLVCSLCFAFTYTLMSSSQSSFFLKISDKSIGGTYVTLLNTIANFAGTYPKFFIFILIDFWTTSKCIVNNNNSNSSNSTTNFSSPITFDNISKELCHEKGGEYIILQDGYYIVSVLSILYGVFMFFVLKKYLIPIERIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQ35</accession>
    <name>A0A8J4UQ35_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TAP42 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008574</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000541">
        <property type="protein sequence ID" value="KAF2070111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQ35"/>
    <dbReference type="OrthoDB" id="10261753at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051721">
        <property type="term" value="F:protein phosphatase 2A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035303">
        <property type="term" value="P:regulation of dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.540">
        <property type="entry name" value="TAP42-like family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038511">
        <property type="entry name" value="TAP42/TAP46-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007304">
        <property type="entry name" value="TAP46-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10933">
        <property type="entry name" value="IMMUNOGLOBULIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10933:SF9">
        <property type="entry name" value="IMMUNOGLOBULIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04177">
        <property type="entry name" value="TAP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="40340" checksum="C579BC74334CCFDE" modified="2022-05-25" version="1">MSESTTTTSTPTTSTTSTTNTLDLSLYQSFVFGQKLYTELDTTALCSNCEEYQDKVKQSIKYLFNASIQVSRQSVFSKNEELEDIRTDLLKYLLIPYYVAELYLKINDVDRVKHLKISKIKVMDFLNQCENLDLIDKEDLNIIHREGKADPVNRREELINRSKKTKEIKQKLEYIKQKREVILKKIGQDDEYEESETGGDEEVDRDFSMLLIKDAIIKAIQLLESIEFELPMLIEIQSIKDQHGGVLPPKAPPKSANIGNFQILPDGRRVMLDKVFRPSHILPSLTPQEAVEIEMRNGGMVSGKGGAESSSKKDSEDEEEEDDDDEKLKKKRDWDDYKDDNPKGWGNSLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQK3</accession>
    <name>A0A8J4UQK3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007892</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000445">
        <property type="protein sequence ID" value="KAF2070801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQK3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="155" mass="18377" checksum="74ABD156B74CBB76" modified="2022-05-25" version="1">METMEFNQDNFFEEEEEIDEDGIKTFTNCSQFLSIIRSFFEDFLKIMVTNGIYFPSISKLVIVLTNIKENDFIDFSNSIKSENFSLVRFLISKQVRLDPVFLETIMRLQFDSVWIYTEDGELEYDIPVVFEWYGDKLLEYLAFVNDEPMSTLTNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UR09</accession>
    <name>A0A8J4UR09_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_007238</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000374">
        <property type="protein sequence ID" value="KAF2071446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UR09"/>
    <dbReference type="OrthoDB" id="1919336at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112:SF22">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION FACTOR A, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="4">
        <location>
            <begin position="48"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="2">
        <location>
            <begin position="48"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="85"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="91"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="131"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2071446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="144" mass="16597" checksum="9EE1EE53368FC297" modified="2022-05-25" version="1">MAKKEEPVSSSDSEGEVETKKVKKTAKKTKKEEPKKKATKRKKDENAPRRYLTAFIYFSKEYRPTVKSENPEAKFGQIGSLIATKWNSMSADEKKRFEELSKEDKKRWERECKEYEAKKQNTKKAAPASDNETDSSDSESDSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4USX3</accession>
    <name>A0A8J4USX3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004558</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000164">
        <property type="protein sequence ID" value="KAF2074116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4USX3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="655" mass="77016" checksum="FE68BC40BA4CD3A1" modified="2022-05-25" version="1">MDKLYRCVFSNLYISTKIFTHVHQIQLNDYSLKYDVIVDAGWMYRNGHIKLLKEKINNTNIKLYVCNKELFSLIANADTCLFIVLFERTKDSALDYLDSKSAYDDFLSNIRNKEVLVYLHKEGHLKRIKFKLVGRMRVDVVEYLVDTGYMVLTPNVLLHNRVFGREYNGQEVNREYNEEEMYLLPDKSVIMHHKREMVELVLKHVAKPISQTYAQQIMAYLYSNPTLYIYELLSPHFDPGIEKTKIEINNQIKVPYSSAAGVIERHLETLRQRASIYQYRSKPIDLVHLIKQDCRGFWMWLLKQGDEAFFSLSTQRITTSLNAVFFYFGDIESRDEMDPSFDSLDVYPDILEAACMHGCVKVIKYFIDSGFNSQRINTRILDHSYTFFKSALRHTSDEYRGIMTDLAKFLFTISCAKILQSCCEHGYVDNLNFYLARYKVPDDSQTYQETITHFYDLALYNNHKHICRALEAQGHPYSDCPDDNLRLIERHGFKYMLDDVRRHIDRVQDSNKTDMILELLKISAIHNDFVGFKFILTEYIKVVKGNIIDVAAMMIAHSSNLAIIDHVYQHNATYFESPATFSLFVKKLYQSSADQNNFQFMEYITNETNSYSPSISEAQLYRKQQKHRKKQLLEFSQQQEIELQLQHKDKKCILM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UTR7</accession>
    <name>A0A8J4UTR7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Snurportin-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_010531</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Functions as an U snRNP-specific nuclear import adapter. Involved in the trimethylguanosine (m3G)-cap-dependent nuclear import of U snRNPs. Binds specifically to the terminal m3G-cap U snRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the snurportin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001175">
        <property type="protein sequence ID" value="KAF2068143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UTR7"/>
    <dbReference type="OrthoDB" id="10003593at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061015">
        <property type="term" value="P:snRNA import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09232">
        <property type="entry name" value="Snurportin-1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017336">
        <property type="entry name" value="Snurportin-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047857">
        <property type="entry name" value="Snurportin1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13403:SF6">
        <property type="entry name" value="SNURPORTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13403">
        <property type="entry name" value="SNURPORTIN1 RNUT1 PROTEIN RNA, U TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21974">
        <property type="entry name" value="SPN1_m3Gcap_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Snurportin-1 m3G cap-binding" evidence="10">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2068143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="215" mass="25199" checksum="784798226BABA694" modified="2022-05-25" version="1">MVYIPSNFQDEWECVLVPNGIRCLVITNHNKTIVRSSDGQIFNTFQSMLPFGSETSREGGTTPSNQHCILDCVYDSDKQNYYVLDILSWKGYMLMDCDTEFRFFWKKTKLMETIEATKRSNQNPFPFLLPISCSTTIEDFEEILYNLNEDLSSKQCQYQPEFLLFYHKESHYEFGSTPLFTSLSIQYLEMLINNLKENDNIEEKEEELDGDEALE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UUU7</accession>
    <name>A0A8J4UUU7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002779</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000080">
        <property type="protein sequence ID" value="KAF2075931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="102511at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14596">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14596:SF72">
        <property type="entry name" value="ZINC FINGER PROTEIN MSN2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="773"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1922" mass="221753" checksum="6FFB2B1AA4495A0E" modified="2022-05-25" version="1">MSIETINDSKTSSDTAATLGGSTSSISSISSTTSIVSSNSNKDSNDKSVRVSDRLSIFYNYFDKELLSEDEMKVEFSRVENKLSTILELYSEPMKNSKDQILSGKIKVKGEEVQISKNVQDFTLELSKVLNLDEISTYIFIKNYSTFILNLNYEKEEFYPLFIEYYSERSSLLCILGSMIRKQKGSSKYFKIIDSFIQKNRSSIESKLLSYLISFQTFTIPNYLKDYKYLQQDYCYYIVKEQLLIIETLFSYSYTKPSMDYPNDLLKLLKKFVIEFSIGNYSTQITNIPSSTSFTTSSSSSATPTTTTTDYSNIKYQDQKEFYQREIISNLERIELVSVLLIFNSNRDQPTKSILQQKDQEIVRVLSEIIANSSKITPAISTCLFYTILNLLPHSTETLQDCLDQGLPLSLLYNPYEYIYNIALYGFSSDNVLLEGYCDVILLFFNRLSEIINFDQIKVFKRVVDIYCLLYEKGGESFSQYLWNNRLNNPIFKYSISPLFLKNNIQSVLSILNSAIYPSTSTLVFQELFNISLNSETEQLSQFEFNTMIQPYLDITNQIENNFGAFVNKEINLPKQKLYRNQLKSFTLVNDNEVEIKYTLHDYIIDIFTVGLPQMNLEMYHVLNDSDTFYICNSYRIAISLFSKMINNDINSRVYFWNLFNQNRIFDSIFQVYFTLCDQLTFTNINNNGMNSKAVTDGLSSNMVIDIISEIISLFTSFSISFSNELLDILSAKEIWSCGPSSILRSQSKKGSFIYLLDLEKCCTSNNNFNNDNNEIDNNSNSNNNNNSNNNNNDNNNNSYILTKRIVQFLNSCFIKNNLSKVKTSNNSNNNQNNNLVNIDSAIAIQIQVLSNYFILLENNNHISTWEIVKEIFNSIQSILSFSQITKSDGLKSLSIEIIKSFSKSTGGNVIQSALINLLDLSSLLSIQNSILRTSDTDSILYSSLSLCKTILESMVLNQQQQEYFMEFINIFFKIKNNDNCLFTTIFGYTTSFTDREDIQICSCNILIYIIRILRNIDSKYCISTIFQNNISDNDSNSDNQNNINDKVNDFIKILYPTENSIYSTQLQTFALQFLVECSVSQVQFFNKILNLVKGTIFQLFKSNCNQETKLSMTLFNLFDNIYQNSIYFGEFTQEILNDNQEFWSCLIQVLKSASNQSNRLLTPQENVFLCLKKSYSLRIISLAVLLDSSIFTKQFQQTYQDQWNNCFRKNIDLLIKNEFFTCIDPEKRNPLNKLSNLLVPLDQLQIIPNSFLPSVLVYNINNIIKSHNLNNNNNDNNNSQQQIDSSKIDSFIDCLQNRNTDLLLSSAQLILSKSFSKFFRVLLHRKVNLLVKSTDSGLVKLVNILIEKSIYVLNNDIKNIENDLLTNLVESISTCTQIISKFNSNNNSFDELLPSFIDLINQMLSKKIDNIDQNIIYHLLSSFLYVIQSKKSITIQTFNQFINSLSILITNKLFRKVLNNNSNSNKSLPSICNISIFILQSFINLLDDSSLLPTILPHQLLDHLIQLLQHSLDTPNDSNSNITSIAIINLMISFCKQPLISESLLVKNILGILSSRKLAYLEYSFDPYISKSNSTPSEFYDQDKKRNPWHKVWSLILQFVITISRSLDTNETLQEQLANFILSHKDRLYCYFDPLNENSIQPQPQYYSKLWFEQIQLITSLLAVFTNYTKRYYLQLSLFNEFLVLSFRTLVSMSIQVSELSFIENNFKPFTRKEKRLNKLIIKELNNNSSNDNSSNSNSSNNNSLLFSNSNNSNSRIFDSLNNRINNSKFKFYIESNYMNIISNCLTILIHINEFIKTCEEFLPKLPLYTNIATFGTICQIGQYIISKQIQFKNLFKNSTEFNDNSSGSKMISIKEITDINTLQERILQVAEKTSLILVLYSIQYQVKEDPIYQCLLHLNQQFNIKSNQNQLLSLLTKIYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UV21</accession>
    <name>A0A8J4UV21_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ADF-H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_010456</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the actin-binding proteins ADF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001125">
        <property type="protein sequence ID" value="KAF2068220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UV21"/>
    <dbReference type="OrthoDB" id="25672at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11286">
        <property type="entry name" value="ADF_cofilin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017904">
        <property type="entry name" value="ADF/Cofilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11913">
        <property type="entry name" value="COFILIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="5">
        <location>
            <begin position="4"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2068220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="140" mass="16038" checksum="E68F35595E0F9F93" modified="2022-05-25" version="1">MTSGVQISNECVPVFNDSLKLGKSIRAIFYKLDQKSEKISVEKTIPRQETNFETIVSELPLQEPRYVTIDYEYMSEANLPKSKIIFLSWCPNDSAIRAKMLHTSSLQSIRRALVGVNFEIQATDVSECSHEIILERINRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UV30</accession>
    <name>A0A8J4UV30_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_002486</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000069">
        <property type="protein sequence ID" value="KAF2076186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UV30"/>
    <dbReference type="OrthoDB" id="442460at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.130">
        <property type="entry name" value="Ubiquitin-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47764:SF2">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEASE FAMILY PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47764">
        <property type="entry name" value="UBIQUITIN-LIKE-SPECIFIC PROTEASE 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="5">
        <location>
            <begin position="264"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="56"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="492"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="500"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="511"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="533"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="595"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="606"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="643"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="691"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="727"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="743"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="764"/>
            <end position="776"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2076186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="800" mass="92172" checksum="B38ED8CB799B1830" modified="2022-05-25" version="1">MTRKRTRGASPHDQPLKVSKLNFSDSLIEQIIDDNKGTHENPLNLDEDDDDIVDVTPTTNGNGKSTTTTTTTTATATPTTEQSTTSTVDILKIMESIKQDNIYLKPNLIAFGSNNPIRSTKPVVFNETEIEFEISLKALQPYQSKSDDVVVIIRYKDISKFSVSFRNSQTDIEINVKEFTFKKGWLKKVSELRSYEAGIPHDIYLRSISKDEFTLMRPQIGLRSEFLNVIINVEDKNASNKTIIARYPHRASSSKCEDITRDVINITSDDLSRLEPFQYLNDSIVDFYIRYIKDKYVDAKDSSRFHFFNTYFYNILTTQSKIEDAYTKISKWTKNTDIFSKDFIFIPICENFHWTLVIVAFAYLDSDKQSSSQKSVILYLDSLHSGLGKITNKIREYLSLEWENKKSKPSNGEIPEKKFNFKNLPLYRCSVPKQNNLCDCGVFLLHYIELFCRNPEKNFDDPLNKPQWFPISEIEQKRESIKTIIKVLALEQDSERQEGDTDSSDSDSDDEKEKEKEEKKVDTENNNNNNQEVNDKMDIEKEKSLDSKKNDLDTSVDEKNENDKSLEIIEKEEKKENENDNDKSLEIIEKEEKSSTVNSNNNNSEKQNENDKSVEIIENNENDKSVEIVENHENEKGTKVEESLLPSSLPLTTNTNDDNEKETKVEESLSPPTINNEKENDKGIKINVNNQDDKSLEIVEKETKVERSSSPTINTKSDNEKEDDMIQINNQNNKSMEIVNSNNKKEEEEAFKSKEEKDLNPQENSNINDISNNKDSNINSFIEKVNQDETTYGFSNSKWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWT9</accession>
    <name>A0A8J4UWT9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Right handed beta helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000843</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000017">
        <property type="protein sequence ID" value="KAF2077881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWT9"/>
    <dbReference type="OrthoDB" id="1046782at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Right handed beta helix" evidence="2">
        <location>
            <begin position="481"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="691"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="101"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="695"/>
            <end position="712"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="712" mass="80087" checksum="3DFF1DB181F8FF2A" modified="2022-05-25" version="1">MKKAFNKLSNKINNFVEDYKNPNEKLPSNQQFNNQKQYNQDPNNQYNNQYNQQYNSQYNNQYNQQPQQQFNNQQQYGQDPNNQYNNQYNNQQQFNQGYQAATQYPQQQQQQQQPQPQTLRKYPSYPQPVNSQQPIQNQQSQQQINLQKPNNHSMMINNNNNLNHQNKDDYKIIQQQYYQTNNRPLPKPPVNNNNNINGIPGAHADGIHDDTQAFSEYLNSCTQNPIIIPSGTYLITSSISFAKSNKTILGSSNVKIIGNISNVWNNEPISEGFINTKEYLFVLTGDNIVLDSLVIVNAIDNPKSSSAFYAWGNGFTLKNCSVDNFNQGLCFGKLKDGSGTIPPNSTFTNLTISNNQITNIIGKHGGSICYGDAIAFFGVTHVVISNNFISAKNGFTPRNGLNAGPEGFIRSSDIQYQNNTIRGDWDYAITTEGSDDAVISGNDIQGANICSIIERGKNIKVCDNKIHIHGRFVQGHTESTAIQFYGVDHGEIHGNNIQGTAKYAILVKPSHESAGGNHTSIQFNTIDGDFFNCIYFGNTNHGVIKKNTILGKNKDNGSVGLQLWYANKLEIHENCIDTPQGTACICSGTDQVNVLSNQMMNSRAGFYICRGSKCVRVENNDLKNVKQTKFDQSHDNQDVCHSQNYGIDCHSPTHNHNNNNVNNGNTVNNLNNHFNNMNINSNVNNNYYNNHPHTHQPQPNYQTFNTNLKASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UX19</accession>
    <name>A0A8J4UX19_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008309</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000500">
        <property type="protein sequence ID" value="KAF2070380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UX19"/>
    <dbReference type="OrthoDB" id="2015333at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="188"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="224"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="51"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="259" mass="27244" checksum="734D68A375703856" modified="2022-05-25" version="1" fragment="single">GAPGAPGAYPPQQPGAPGAYPPQQPGAPGAYPPSNSGYPTATPPGAPGAYPPSASGYPTATPPQGQYPGAYPPQGQYPGAYPPQQQYPGAYPPQQQYPGAYPPSASSYSSYSSSTTYGAYPQTGYPAAGGYPGAYPPTGGYGAATGQSMCASTLLTSAYVRPTYTYPSVYLRSAAFILPTGVAPHIAAKMMRASSTFRMFDTNCSGTLSKKEFKKCLKYLGYYFSKGQSKLIFFSIDKNYSGSIDEREFVEWMVTVSPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXA8</accession>
    <name>A0A8J4UXA8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carbohydrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008006</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000460">
        <property type="protein sequence ID" value="KAF2070680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXA8"/>
    <dbReference type="OrthoDB" id="10635035at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019028">
        <property type="entry name" value="CBM_49"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052879">
        <property type="entry name" value="Dd_Spore_Germination_Stalk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33239">
        <property type="entry name" value="CELLULOSE-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09478">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01063">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035184406" description="Carbohydrate binding domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate binding" evidence="2">
        <location>
            <begin position="53"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="153" mass="17422" checksum="2481C748383EA0CB" modified="2022-05-25" version="1">MRSVTIIVLILTIFHSTQAWHAECGTEICVDGQFCMDRNGNETCVKSSMGSFIGISNIPENTWVENHQEYTLFRSYIINFGENIRHITIGTDTPLELKYNESSIWNINYNHKTLSEFATPPYQAGIKGGKSFGWGFIIKGKACPQMKIRYLVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXI6</accession>
    <name>A0A8J4UXI6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Insulin-degrading enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_000071</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the peptidase M16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000002">
        <property type="protein sequence ID" value="KAF2078571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXI6"/>
    <dbReference type="OrthoDB" id="952271at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000012">
        <property type="entry name" value="Protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050626">
        <property type="entry name" value="Peptidase_M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032632">
        <property type="entry name" value="Peptidase_M16_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054734">
        <property type="entry name" value="PqqF-like_C_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690:SF18">
        <property type="entry name" value="INSULIN-DEGRADING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690">
        <property type="entry name" value="NARDILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16187">
        <property type="entry name" value="Peptidase_M16_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22456">
        <property type="entry name" value="PqqF-like_C_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="9">
        <location>
            <begin position="31"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="10">
        <location>
            <begin position="187"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 middle/third" evidence="11">
        <location>
            <begin position="371"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="Coenzyme PQQ synthesis protein F-like C-terminal lobe" evidence="12">
        <location>
            <begin position="760"/>
            <end position="859"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2078571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="950" mass="109023" checksum="5FC2DA12DB2BD18A" modified="2022-05-25" version="1">MVSAIDNIEIIKSPNDKRNYRYLKLDNQMEVLLVSDSAIDNAAVSLRVGVGSYLNPPEALGLAHFLEHMLFMGTEKYPIEKEFAQFISENGGSYNAGTSSVYTVYYYNINQASLKESLDRLSSFFICPLMSDSSTNREINAINSEHNGNIQNDVWRVANLRGMAFKDHPASRFSTGNFETLNKPGMRDRVVEFYNDNYSSNLIKLSIVGPQTLDALEEWTREYFSPIKNKNLPDPVHPPLVLDKSVYMHYVPVKSKNTLRIEWPIPDPAYTFTNFSKHTGPIISHLLGHESLGSLLSVLKKKNYAYGLSTYAHPTSKTIFSVVNDIDLTDTGLENIDEILNLNFQFMKLLDNIPKYVVEETIAHNTITWNNASKSRALDYSRSIVENLYYTKKPENVLKLRYFSDEIDLEEIKTLAQKYLTPENMLVVVGSDKFKGKTDLVAEYYGTEYSYVDITPERVEKWRSGFPTDPSLYIPAPNQFMPKKFDIKNEQNTTAEIPMPSLVYSQNGIDMYYSPDTWFNVPMAQIKVRFESEHMSGTRTTTNLFLFEKIAKDILNEEYLYDMILSGISVKFINWLTHFELKVSGYDDKIFFALKTAFSLLYAFDCSPECFSRAVEKVSTRYINYQLETPYQLAIKESNLYLSTVSSENQDKVNYLSQLKREDFVQWIRNYFKTISFTFFIFGNVTKEECVDFCENTVSKFDRTPTPKADIARCRKVFLPKGVEHQVRRYTADAAQTNGAIETTVQIGVHNIKDIAIVTLLTGITASSFFDNLRTKQQLGYVATSLSSFVGNITFRFIVQSNVCPPHQLVAKVKEFFPTLIDTIETYRADQGFEKLVESTKDTLSRRKQNQIDAHSLFWDSFTMYNEFNFTPKVVKQLETITIDDLLTFVKRHFLTKSELRIFSTLIYPKTFEIPTNDDPEIIILNDFGTFRAKSLLCPAIQNPTSPIQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXZ0</accession>
    <name>A0A8J4UXZ0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GP-PDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006926</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000341">
        <property type="protein sequence ID" value="KAF2071760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXZ0"/>
    <dbReference type="OrthoDB" id="197419at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08561">
        <property type="entry name" value="GDPD_cytoplasmic_ScUgpQ2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43805">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43805:SF1">
        <property type="entry name" value="GP-PDE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="392"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="2">
        <location>
            <begin position="71"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="411" mass="46765" checksum="4307200C3C9F1109" modified="2022-05-25" version="1">MGLKSLFYLLIVLTCIFLYYQNNVNPLFQHPRSKPIIFEKFHRSVDTNNIGNNSKKTTVATVGYDNDFYRPLILAHRGSRYLLPENTVLAFKTAVDLGADVIETDVRLTKDGVLVIHHDKLVDRTTNGVGDVENHTLEELVALDAGYRFSPDNGTTFPYRGKGVKIPTTRQMFEQLDPSVQLNIEIKENDVAVADALWTEIERAFESSARKPRSIVVSCRYCVPTLHIRKLAAEYAAKKGLDSRPITTSACETDATRFVVLSQLYLAKLYYTLYPTFHFESFQIPTNSGPIRLDTELFIDAANYFGKHIHYWVINHKDEINRVLDISNIDGIISDRADRVAQIYKDRGIFSKSLVIPKPLPTNTTGTYFIPSIDIEEVHTCISLTCLALQRIHHIILSIILTYIAYRVFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYA7</accession>
    <name>A0A8J4UYA7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Signal peptidase complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_007022</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPCS3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000352">
        <property type="protein sequence ID" value="KAF2071668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYA7"/>
    <dbReference type="OrthoDB" id="10261524at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007653">
        <property type="entry name" value="SPC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12804">
        <property type="entry name" value="MICROSOMAL SIGNAL PEPTIDASE 23 KD SUBUNIT SPC22/23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12804:SF0">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04573">
        <property type="entry name" value="SPC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016089">
        <property type="entry name" value="SPC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2071668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="171" mass="20129" checksum="8338A053ACC64699" modified="2022-05-25" version="1">MYSITQRANTIICFGGMVLFGVLLLNVLSRAFFPNHVDVDIKLNSVTRLNKRNSIEYAEVHFDLQTDLRPLFNWNTKMLFLYVVAEYQTKQNVISQEVLWDHILMDKEKANIDVKNIFKYYLVDQGLGLRNNTINLTFNYNVIPISGLLTRHKDGSHQFKLNDRYLLDKYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYM4</accession>
    <name>A0A8J4UYM4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Coiled-coil domain-containing protein 58</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005544</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000223">
        <property type="protein sequence ID" value="KAF2073155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYM4"/>
    <dbReference type="OrthoDB" id="5593818at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="15421" checksum="F95BB83792F3FD3E" modified="2022-05-25" version="1">MESADHNNSSSSSNTSNNISSLRVPKTCNYVELKDFLVSARKENTNIEMNKIRDIDSECRKVWQDIETRSILRKQVITNCIQETELSLQKAKESNNDSLIRSEKQKLNFLNMEKNVEDILVDYNLKIFQKMCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZ13</accession>
    <name>A0A8J4UZ13_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BPL/LPL catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_004814</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the biotin--protein ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000177">
        <property type="protein sequence ID" value="KAF2073890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZ13"/>
    <dbReference type="OrthoDB" id="16525at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004077">
        <property type="term" value="F:biotin--[biotin carboxyl-carrier protein] ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16442">
        <property type="entry name" value="BPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004408">
        <property type="entry name" value="Biotin_CoA_COase_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00121">
        <property type="entry name" value="birA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835">
        <property type="entry name" value="BIOTIN PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835:SF5">
        <property type="entry name" value="BIOTIN--PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="4">
        <location>
            <begin position="122"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="284"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2073890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="407" mass="46040" checksum="06872FF08F1A2D20" modified="2022-05-25" version="1">MMNSLLKNKTATDIGGNLIKHTFSSMSSNSGAITIPWDIRNTVLTLYSNSSPRLDINEAQQRFATPPFPTTKVVRLYSSLADAEKDRESSSDKDTDTETLRVVVANDYRHQRQQHTYQFDVAQYFGAIQTHLFGQNVIYAPLVSSTQTLCLEYFAYTQQGLVMLVDQQTSAKGRGNNKWVCPLGSLLFSYKIRQSDGNKLPFLQYLVGIAMVEAIKSHPVAKDINVRLKWPNDIYGGNLKIGGILCQSNYLNNEFDVVAGVGLNISNDEPTTSLNKMIRDHLASSTSTTTENSSDSKQEQQPQIYITREQVLSKFFTIFEEMYIQFNKQGFDPFVQRYLNVWMHSNQIVTIKENDKKVKIIGLEKNGFLKAVEVDPISLNEISPMTFELHPDGTSFDINELVLKKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V041</accession>
    <name>A0A8J4V041_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Enoyl-CoA hydratase/isomerase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004791</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000176">
        <property type="protein sequence ID" value="KAF2073903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V041"/>
    <dbReference type="OrthoDB" id="1737613at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003860">
        <property type="term" value="F:3-hydroxyisobutyryl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045004">
        <property type="entry name" value="ECH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032259">
        <property type="entry name" value="HIBYL-CoA-H"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004127">
        <property type="entry name" value="PRK05617.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43176">
        <property type="entry name" value="3-HYDROXYISOBUTYRYL-COA HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43176:SF7">
        <property type="entry name" value="ENOYL-COA HYDRATASE_ISOMERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16113">
        <property type="entry name" value="ECH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl-CoA hydratase/isomerase" evidence="2">
        <location>
            <begin position="78"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="427" mass="48453" checksum="426DB7C617438D08" modified="2022-05-25" version="1">MTRDNSGNTNNNNDDELNINNTLSELSDSKESTTTTTTTTATTATTATTDNSNVYIYKSYSICNDEKILVQEYRNGVAMIILNRPKALNSLDIEMLTSLKNILRKFEGDNRIKVVLIKSIDGSRAFCSGGDIRQFASYQGHFDSLNHFVKTEYCMDYIIHTFPKPIITIVNGIVMGGGVGLSVHSNYRVVTENVVWAMPENVVGFLPDVGTTYFLSRLGAFGLYLALTGDRIGARDLISTKLASHYIPLNILDQVLQEISTDDNVENHRQIGFILNKYSKTLPLEGKEMESVYLKYKDSIQRCFGYNPKSVQDIMNRLEEEKIKYNTEWGSKTIEKLLNSCPLSLLVCFESLKRALHLDIEQVFEMETRLGTRLAIRPDLYEGVTKSMVKRTKPNWSFPTIDSVPIDYFNSLFDPFENQSHEFDINY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0K8</accession>
    <name>A0A8J4V0K8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor eIF2B subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">eIF2B GDP-GTP exchange factor subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_002841</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000083">
        <property type="protein sequence ID" value="KAF2075855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0K8"/>
    <dbReference type="OrthoDB" id="269919at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10470:FF:000009">
        <property type="entry name" value="Translation initiation factor eIF2B subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051855">
        <property type="entry name" value="eIF2B_beta_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45859">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45859:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2075855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="348" mass="39180" checksum="44816EBC42785C0D" modified="2022-05-25" version="1">MERQLETFILHLKRKTVAGSYQVSRATAEVLRHYISKEKWQNAQGLIEQIKAVGKRLIDAQPLEFCIGNIIRRVLFIIREEYLTMMKSNTNSDSASGAKQHTLLTYEDPEDIDYSESFPRLKASIIDGINELIDELEGLHRNVAEQAIEHIHANETIMTLGCSKTVEEFLKEAGRKRKFGVICVETAPSLEGHKTALSLSKAGIDTTLITDSAVFAMMSRVNKVIIGTHAVMANGGLVAQSGTHTLAVAAKYHSVPIVVCTGLYKLCPLYAYDQDTFNSFGSPGEYLKFEEAEYLESNVHSYNPTFDYIPPELVNLFITNIGGHNPSYIYRLLQEYYDPRDIFEDEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V169</accession>
    <name>A0A8J4V169_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008514</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000531">
        <property type="protein sequence ID" value="KAF2070172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V169"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="150"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="248"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="358"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="200"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="73"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="179"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="259"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="358"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="383"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="426"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="471"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="518" mass="59049" checksum="AB8E676B542A4CD3" modified="2022-05-25" version="1">MTDKNLKYTPPNSPLNINNSNNSNNSNNKDIQKTMVPEPSENNSNSNSNSPVKNMINQKKRSFDNDDLTNEDNTNKKAKESNEDDSRSRYNLNMLLKASQSPNQSPVPSPRGSPKSSNILKILVSKDQEYEIPTHFVTQNQQQTLSNINLKSLSSPGSSSSTPPTLSNSNQNIPTLLIPSNSSSSSSTSTTPNTNDVKKYEELKMEKSLMEKDYQNQIDQLKNQLKQQQQQQVQILLQLQQQQQHQQLQLQHHQHQQQRKSQSGSSSPKATVDPVEFLLHELKEIKSQQLEMKNAIAMETMQHLSLKMEEFKKAFIEYQTFFENRLNTTDERMHSYCLKVAELDKIIYHYFYPTSGKIPSSPSVSSHNQSSKENSPRDHSPHLYSSHSPRFQHPSAPTTPTSKSQPITSSPLPEKPTDYTKKPNPILQQKLQQRIHLQEQQQAAQQQQIGATQQQNQQQSTSNPQTPQPLSPTHFSTSSPQNKGLHPSYPPAYSIDMYHDSRYYPYPSHPYPPVSYYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V173</accession>
    <name>A0A8J4V173_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dual-specificity kinase</fullName>
            <ecNumber evidence="2">2.7.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_008492</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.1" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000528">
        <property type="protein sequence ID" value="KAF2070187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V173"/>
    <dbReference type="OrthoDB" id="9332038at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14210">
        <property type="entry name" value="PKc_DYRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.10.30">
        <property type="entry name" value="DYRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042521">
        <property type="entry name" value="DYRK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF22">
        <property type="entry name" value="DUAL SPECIFICITY TYROSINE-PHOSPHORYLATION-REGULATED KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="477"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="18"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="181"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="47"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="104"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="212"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="265"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="308"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="348"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="367"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="381"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="391"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2070187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="788" mass="85729" checksum="A8E86380F3E027B9" modified="2022-05-25" version="1">MKSSLSQRIAARRSVCLSSAPVFNSNGDFIDTTSSPSKQSKEEPTFSSSGSSNSNSSKERGTTTTTTTTTTTTTTTSSSAGNTPTKKTSDRPSIKPGFRKAKESWTTTTPSSSITGSSSSFSTKKTTSNSSSSSGSNSSGATSNSINKSTSSAFIGSSASSSSSNLMDDSVQIKHTAASLAKMKVTSQTISERETRLKSRSVSVSNGSMFIPPRTSTPTNTTPTKPSSSSSKSTTDSPKETTPTKSSIDDVFARLAAVTKPGMKSRSQSVSAGSQPSSNSSSSSSASNNSTKESTSITNKIIPKILRSTKSSSTKASTLDKKETPPKPSSSSTTTIQPVKSILKNSHSSSTLSSSTTLLPNIKDTTNSINNNNNCNNSLSKTQSLNPVAGSTSSSSSTNTTPTKPCTTIMTPSVALKLYINELTLSEQSEILDYTQIFFTGNTNKKVKFNPSLPNNGYDNEEGEYKVIEHDHIAYRFEILSILGQGSFCQVVKAYDHKTTQMVACKILKNQKIFHNQALTEIKILEYLKNNDPHSTANIVHLNNHFYFRNHLCITFELLSMNLFDFLKMNHFQGFNVSLVRRFAAQILTSLKFLSKRNIIHADLKPENILLKSPTKSGIKLIDFGSSCFENEQIFTYIQSRFYRSPEVIFGLRYDKSIDIWSLGCILAELYTGTPLFPGSDETEQLAMIIEVLGAPPAHIINSIEQSRRSLFFYQQEEGGEYIPRQFKNTSTGESFGVGSKTVGDLIKSSDEGFIDFISQCLKWEPKDRITPEDGIKNHPWIVELNQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V200</accession>
    <name>A0A8J4V200_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">4a-hydroxytetrahydrobiopterin dehydratase</fullName>
            <ecNumber evidence="3">4.2.1.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">4-alpha-hydroxy-tetrahydropterin dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_002714</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin = (6R)-L-erythro-6,7-dihydrobiopterin + H2O</text>
            <dbReference type="Rhea" id="RHEA:11920"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15642"/>
            <dbReference type="ChEBI" id="CHEBI:43120"/>
            <dbReference type="EC" id="4.2.1.96"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pterin-4-alpha-carbinolamine dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.96" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000078">
        <property type="protein sequence ID" value="KAF2075958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V200"/>
    <dbReference type="OrthoDB" id="277398at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008124">
        <property type="term" value="F:4-alpha-hydroxytetrahydrobiopterin dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00914">
        <property type="entry name" value="PCD_DCoH_subfamily_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.20">
        <property type="entry name" value="Transcriptional coactivator/pterin dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00434">
        <property type="entry name" value="Pterin_4_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036428">
        <property type="entry name" value="PCD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001533">
        <property type="entry name" value="Pterin_deHydtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002018">
        <property type="entry name" value="PRK00823.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599:SF0">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01329">
        <property type="entry name" value="Pterin_4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55248">
        <property type="entry name" value="PCD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2075958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="100" mass="11519" checksum="B84D214FE073B0BF" modified="2022-05-25" version="1">MAPTKLTQEQRSTLIPAGWTLVEGRDAIKKSFSFKDFNQAFSLMTRVALVAEKMDHHPEWFNVYNRLEVTLSTHDCQGLSMNDINMANKIEEFVSEILKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V372</accession>
    <name>A0A8J4V372_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Sulfiredoxin</fullName>
            <ecNumber evidence="2 10">1.8.98.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_009219</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>S-hydroxy-S-oxy-L-cysteinyl-[peroxiredoxin] + [protein]-dithiol + ATP = S-hydroxy-L-cysteinyl-[peroxiredoxin] + [protein]-disulfide + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17545"/>
            <dbReference type="Rhea" id="RHEA-COMP:10593"/>
            <dbReference type="Rhea" id="RHEA-COMP:10594"/>
            <dbReference type="Rhea" id="RHEA-COMP:13681"/>
            <dbReference type="Rhea" id="RHEA-COMP:17976"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:61973"/>
            <dbReference type="ChEBI" id="CHEBI:61974"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="1.8.98.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the sulfiredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.98.2" evidence="2 10"/>
    <dbReference type="EMBL" id="AJWJ01000657">
        <property type="protein sequence ID" value="KAF2069464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V372"/>
    <dbReference type="OrthoDB" id="10023328at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032542">
        <property type="term" value="F:sulfiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16395">
        <property type="entry name" value="Srx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1530.10:FF:000001">
        <property type="entry name" value="Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1530.10">
        <property type="entry name" value="Conserved hypothetical protein from pyrococcus furiosus pfu- 392566-001, ParB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016692">
        <property type="entry name" value="Sulfiredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21348">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21348:SF2">
        <property type="entry name" value="SULFIREDOXIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017267">
        <property type="entry name" value="Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="ParB/Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="5 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ParB-like N-terminal" evidence="13">
        <location>
            <begin position="11"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="68"/>
            <end position="71"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Interchain" evidence="12">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR017267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR017267-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR017267-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2069464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="107" mass="12162" checksum="EC37AF9A3A0E3522" modified="2022-05-25" version="1">MSIYTSLISVTEMPISVIHRPLPSYLDEEKVISLMETLKKNSEDVPPIDVNWVVGKNPDNNYYMAFGGCHRWEAHKRLGLPTIKARIVKSTPQDIKVYLGSSTPEFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4A3</accession>
    <name>A0A8J4V4A3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_001296</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000030">
        <property type="protein sequence ID" value="KAF2077368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4A3"/>
    <dbReference type="OrthoDB" id="276546at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02932">
        <property type="entry name" value="OYE_YqiM_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044152">
        <property type="entry name" value="YqjM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43303">
        <property type="entry name" value="NADPH DEHYDROGENASE C23G7.10C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43303:SF4">
        <property type="entry name" value="NADPH DEHYDROGENASE C23G7.10C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="6">
        <location>
            <begin position="55"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2077368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="424" mass="47743" checksum="85AFB2F602D2774C" modified="2022-05-25" version="1">MVQQQQQSQQQEIIQHVYDKELPIQQFSYQHLREKPNPTYQHNGAVKQGEKVPLLFTPYKVKDLQLINRVVVPPLCMYSAVDGFMNDFHLIHLSSFAKGAAGLVIFEATAVCPEGRITYADAGLWKDDHIAPMKRIVKLVQGFGSKAGIQLAHAGRKAATIPMYLDSSKGELGKDDPNFWQAVGPSPISFSPENNFQVPHELNEKEIENVVHQFKMAASRCYEAGFDFIEIHGAHGYLISSFLSSTSNKRTDKYGGDFEGRSRLLVEIIKAIRTVWPTEKPLGLRLSCNEWLDESANGWTMKDTLRLAKLVKSLDVDLFDCSSGGNNIKQSIRVRKFYQTPFSEAVKKEVPELTVAAVGLITTGEEAELVLQKNQADLVMVGRGFMRNPQWTYQAAAELNTKIDFVLQYEPANRTFNFLKPEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4S8</accession>
    <name>A0A8J4V4S8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA N(3)-methylcytidine methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_001151</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">S-adenosyl-L-methionine-dependent methyltransferase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the methyltransferase superfamily. METL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000026">
        <property type="protein sequence ID" value="KAF2077533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4S8"/>
    <dbReference type="OrthoDB" id="417697at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013217">
        <property type="entry name" value="Methyltransf_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026113">
        <property type="entry name" value="METTL2/6/8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22809">
        <property type="entry name" value="METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22809:SF11">
        <property type="entry name" value="TRNA N(3)-METHYLCYTIDINE METHYLTRANSFERASE METTL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08242">
        <property type="entry name" value="Methyltransf_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037755">
        <property type="entry name" value="Mettl2_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 12" evidence="5">
        <location>
            <begin position="118"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR037755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2077533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="321" mass="37387" checksum="417EC4F64C837A79" modified="2022-05-25" version="1">MTNSSSNNDINTNSKQYSKDQDHSYNFLYPIIDDAMGIARLYGYIDKESQKLVSPQLIERYEKEADRFWDKFYKKNNNNFFKDRHWLTREFPEVLQHADYKKTGDEEVDAKHRLTMFEVGCGVGNTTLPLLELNSNLYFHSFDFSQHAVKLLAQAVEENEGYRGRCVSFVFNAIDGYDSMPVKAEPNSMDLVIIIFVLSAMDPKTIPSCVDMCHKVLKPGGKVLIRDYAIDDMAQNRFDSTNINEIDQQVIASGSKNKLGDNFHVRFDGTRAYYFSLEILESLFKEKGFKTEQNIYVERKVFNRKHNNLMERKFIQSKFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5G8</accession>
    <name>A0A8J4V5G8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">CCCH-type zinc finger-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_004175</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000143">
        <property type="protein sequence ID" value="KAF2074507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5G8"/>
    <dbReference type="OrthoDB" id="10259600at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036002">
        <property type="term" value="F:pre-mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039171">
        <property type="entry name" value="Cwc2/Slt11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048995">
        <property type="entry name" value="STL11/RBM22-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089:SF6">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21369">
        <property type="entry name" value="STL11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18345">
        <property type="entry name" value="zf_CCCH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="9">
        <location>
            <begin position="150"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="222"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="150"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="296"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="305"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2074507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="398" mass="44575" checksum="18F9AC302A8880DF" modified="2022-05-25" version="1">MYKSKQKEETPQQQDFPIACESCLGDSPYMTMTKEEFGKECKICTRPFNVFRWKPGRGTRFKSTEICKMCAQVKNVCQVCIHDLEFGLPVQVRDAALASFSGQQTAVTEKGIEYQAAMNQKMLDNGEISYDNFQPTEVIKQLARTAPYHKKNQPHVCTFFLKGNCNRGDACPYSHSAEQHTDPELAHQDIRDRYNGINDPVAERMLKRAQYPPKPPLDKSITTLFLGDLDFEKVKEEDIKAHLFPYGTLKKLKIVTAQNCAFATFNTRQEAETAISQLYSKFNINENDIRLNWSKANKGKPKVMSNNNNSNNSNIASTETTTTETPNTAAETTTTTTEATETSNNTDKTPAPPKPAGVKNFPTFKFNAVPPPSKTNTNTTKPVYSSMDPSSYGGKYAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5N4</accession>
    <name>A0A8J4V5N4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Polyadenylate-binding protein</fullName>
            <shortName evidence="7">PABP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_003871</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds the poly(A) tail of mRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the polyadenylate-binding protein type-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000128">
        <property type="protein sequence ID" value="KAF2074807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5N4"/>
    <dbReference type="OrthoDB" id="19742at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12378">
        <property type="entry name" value="RRM1_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12379">
        <property type="entry name" value="RRM2_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12380">
        <property type="entry name" value="RRM3_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12381">
        <property type="entry name" value="RRM4_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000003">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000091">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.10">
        <property type="entry name" value="c-terminal domain of poly(a) binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036053">
        <property type="entry name" value="PABP-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006515">
        <property type="entry name" value="PABP_1234"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002004">
        <property type="entry name" value="PABP_HYD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034364">
        <property type="entry name" value="PABP_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045305">
        <property type="entry name" value="RRM2_I_PABPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01628">
        <property type="entry name" value="PABP-1234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00658">
        <property type="entry name" value="MLLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00517">
        <property type="entry name" value="PolyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63570">
        <property type="entry name" value="PABC (PABP) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51309">
        <property type="entry name" value="PABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 6 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="10"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="98"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="188"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="284"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="PABC" evidence="10">
        <location>
            <begin position="488"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="434"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="442"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2074807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="564" mass="62133" checksum="33C7F049DF66BE4E" modified="2022-05-25" version="1">MANTFTPISPSLYVGDLLPEVSEQTLFEVFNQVGLVSNIRVCRDTNTRRSLNYAYVNYYNAGDAERALDTLNNTPIRGKPCRIMWSQRDPSLRKSGVGNVFIKNLDKGIDHKALYDTFSAFGNILSCKVVTDDNNVSKGFGFVHYESQESADKAIEKVNGMMINGQKVFVGPFKSSKERGQPTEIKFTNVFFKNLADEIDSEKLKDLLLPYGPITNITIMVDEKGKSKGFGFANFESPDSAKNCVDNENGKVMFGKPIYAGRAQKKIEREAELKHTFETKYQGVNLYIKNIDDSIDNDKLREVFNPFGTITSAIVMKDDKSSSSKGFGFVCYTSPDEATRAVTEMNGRMIGTKPLYVALAQRKDIRRAQLEMQHSQKFKSGGIRQQPMAPAYSGGPVFFTPAPGPMNPQVVYQQMMPRAPRTWNGAPVGVPQGQYPINYGVRGQPRPNGPRANGPRPNGPRPDSAQPIPAQPIETAAPAAQPAAAQTEAPLTLASLNGLPRDQQNVALGEHLYPLIQSTQPELAGKITGMLLDSLSVDELFTLTQRSDLLQEKIKEAIEVLGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V668</accession>
    <name>A0A8J4V668_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_006176</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000273">
        <property type="protein sequence ID" value="KAF2072509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035249522" description="EGF-like domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1313"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="7">
        <location>
            <begin position="413"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="6">
        <location>
            <begin position="632"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="5">
        <location>
            <begin position="785"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="4">
        <location>
            <begin position="1071"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="994"/>
            <end position="1017"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2072509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1356" mass="150475" checksum="D41CC5E535E9B7E4" modified="2022-05-25" version="1">MCKLILIVFLVVLFGWIGEGFNLTDLTNSLVNDKYAELDNNGVLTCVFEFTILLQDDDGVTLLGTTPSSPHFYIVDQSATKTLYQLSAALPVGTGPLLLDVSNGASNSQLSLPYECTAPPSVIDIQFIDFNNQLIYTKSQGQMNPFLIFRVKNFYKYVPPKLNFSFPFVYYAGVGNLGSQVYVLGFRPTNFNIFQDFSMNVSYSGSSNVQIYPIKGLAAPSEYDEVELVYDFYPLNSNNDTEYGNLYSVFQVKTNNTDLMSTYEDIYSGSVFPKPVKSESNGFTTYYNVFYPTTTKSVSLLTFTQENNPTNYSQAFNFKNYYSPYFLDMPTLNMTVNSQHTYSFSALTNATFQFNKNSLFLNSVTYPYGYANGTTKSYYRQFSLYLSPYFNSTIQVNNNLIVIPFTVTNGIGNDYLAPVIQAINVIPLANSHLVIVRMTITDDMSGFQNFGSDVGKYTDIVSGNSTNGIYEFLVNIKQVYSLNSFGTICDFANNCKLYDVFNFFNVNQSILNYRVFNFSSITNVEFEYQNIDVSNKSVDNRLFMYASNRYQDEYPSIYQYLSKGDIDYSNFLSPIHGVWNPTASRYEFSLTIEKNTIPGTFAYIMYTDTLIYSPHIEALFPNAKLNITTAQGDMFGPVITNINFPRGKSNTVVISPTDQYTTLEWILTIQDEVNGFKKGMVSILSDTDGVRYNVTLNETTRIGGDSKLGQYSIKVQVNKVCKSQYFSFGYIYLEDTNSYFSNYQNISLYSFGSNEFNSFYKLIGDIKSFGIQTVCQDSDSDSSPPMLTSFVYSPQVINPFAIDPSARTITFQIEAIDSSGLLLAATPSVYLQDYSVNVYQQTCSLTTSNSTWALYTCVVLTKYGFGYPGGIKTSVYGLVDNKGNFNGYSMAALEEAGINTLIDVDPVFSLGILSCTEISRFGTANVIVRGSGFLPKDRVEIDYGNGTIALLSPSAFSSSLIQFLNLGSINVNAVYLTVKRNTLTFSNRFKVLVQQPPPEPSSSESSSHSDSEIPPNPNQECFSGCSGHGECTVKGCVCTGNWMGVDCSSQIIIVNPTINNTNPSTNYTIPSTNEGNKVFYAIISLVGLNELDSGGQVVHQYSFDQWIVGGKGSIVPRYSANSTTLYSTNLTNALDQSITTLNVSIDYFVESVNITFANQVLTMNPYSLKYSVNISSYTFANSLNSLQLIMTASIESPDSDCSSLQTGNTVESESEFVKMQVQDHSLYGRFVKRGLIDGRVQSVTNTINAQYNNNHPDTTPTKSQTYIGINIPYYLKLAQLDPDFSVLVDTKDASQSSNATCVSNAKKLTKAQIAGIVVGCAAIVTVGAVSLAYYIYIKRRDASFSKSISHKMDSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6H2</accession>
    <name>A0A8J4V6H2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000244">
        <property type="protein sequence ID" value="KAF2072839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6H2"/>
    <dbReference type="OrthoDB" id="22912at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021837">
        <property type="entry name" value="CfaA/B/C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39523">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39523:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11912">
        <property type="entry name" value="CfaA_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035283014" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="24444" checksum="9C74A42D61307F86" modified="2022-05-25" version="1">MMNKTTITILLISLLFIIINTRASFIKTDIITDVDHLLALQGAQSPMLNFLPFTNSDCTGAAKGIGFGVQEIGCMQNYTNSILAYTFYNSTTVNLTISYDDFCDNYHDSQTFELNQCTESLVNTAPQPVYYIVYQSNTPKFPKKSVVIKEMAQDCLKIQSYWFVSNNTLVNVNAQESVNFYCTPQGVPYKEQCTTSGGCFTNVVDLQCSASNTFQVECT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V709</accession>
    <name>A0A8J4V709_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005256</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000204">
        <property type="protein sequence ID" value="KAF2073439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V709"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="66" mass="6924" checksum="D82513E9AA33B9FB" modified="2022-05-25" version="1">MTIIESLGNLNTVNAGSSLATPKQNSNSKISKSHETSNSIPSSNQVMFFSPPPFPDQGASVSCNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V797</accession>
    <name>A0A8J4V797_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">U3 small nucleolar RNA-associated protein 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_004957</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
            <location evidence="1 5">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the NRAP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000186">
        <property type="protein sequence ID" value="KAF2073724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V797"/>
    <dbReference type="OrthoDB" id="10251401at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032545">
        <property type="term" value="C:CURI complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006409">
        <property type="term" value="P:tRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005554">
        <property type="entry name" value="NOL6/Upt22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035082">
        <property type="entry name" value="Nrap_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035367">
        <property type="entry name" value="Nrap_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035368">
        <property type="entry name" value="Nrap_D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035369">
        <property type="entry name" value="Nrap_D4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035370">
        <property type="entry name" value="Nrap_D5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035371">
        <property type="entry name" value="Nrap_D6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17972:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17972">
        <property type="entry name" value="NUCLEOLAR RNA-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03813">
        <property type="entry name" value="Nrap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17403">
        <property type="entry name" value="Nrap_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17404">
        <property type="entry name" value="Nrap_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17405">
        <property type="entry name" value="Nrap_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17406">
        <property type="entry name" value="Nrap_D5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17407">
        <property type="entry name" value="Nrap_D6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Nrap protein" evidence="7">
        <location>
            <begin position="233"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="8">
        <location>
            <begin position="410"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="9">
        <location>
            <begin position="551"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="10">
        <location>
            <begin position="726"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="11">
        <location>
            <begin position="884"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="12">
        <location>
            <begin position="1048"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="349"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="33"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2073724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1224" mass="139668" checksum="C635A26C74F93E44" modified="2022-05-25" version="1">MAPSTKRKASSSQDASLKKNKTEEENSILALGMDDDYLDDGDVNEYDDSLYGGEAADDSGSDGDDENQDYIDGDDDEEMLDDSEAAVGRPTILEEKNDKNDKRFVNLKVKNTSITSDKPSVAPTNEEIQNLKETQDLFNSNLFRLQLLELFKQVKVDYSKLTSLESALHQIKSIIEKMPDQDVDVYSNNIDNVKIFDSKGESKDVSIKFTKPTNVDVIGSFMSRTVLKQNSNVDMIVEIPSPVLNSKDANDYKYFTKRNLYIWTIRQELIKHARFVDTSFTNFDGDINKQILVIKPKEDAKTGAKTKFTIRIIPTIAKETFKYQNKFNPNTNCILLQNETNQFKIETFKEDDSSAQQQPLPTTDKVDKKKKPFDSIQRSPFYNNAILEDIFIADHMQLIYSKTTSAPVLVDTILLLKLWLDLKQIPTINGFHLTMILIHLYTNGKINKSMSSYQAFRMVMVFITRDFQSKPYLYMNLDEESPIASTPLVYREAFEKLYPVAIVDPSGMLNVASRVSVWGLRTLVQEAKLVLENLDSGNGFEEIFMTKNHFYLSFDLVIKVQLDKDNCLNTTPTNDYYQVDRYLEHKIYTTLRKAFSKRIKKISIVNNNAQKNATWTTDMPQPEDRIVTVGINVNPENWLSVIDLGPAADHVNAAKFREFWGAKSQIRRFKDGSISEAVTWTPKNNLRHLVLEEITKYILNLHLKVPTTNVSSIVHQLDALIYNPEVEDQTLLALKAKDTLVNTIHSLNLPLAIETFPSMAPGLRYTSVHVTQDTTFTNNEPLTVLLHFEQNKNWTNDIESINALKTAFILKIARELQDTPYNPKLSKEYVDLQCEGFLFRLIPYYPKEADFMREQWKVENLPQLELLQRSTLHHTYVHSLHTAHPTYGVTVRLALRWIHSHLFTEYIDHKTIELLVASLYRVAENSSQLVPQTALSGFLRFLFLLYTFNFEEKPVIVDHLDNITKDDLNKIQSTFDLQKQSANPPLIFIATDKDRTNTWFRSTAIKDQAIFNKIKLFAEKSIHLIESNYLENNSTFNWLSVFETSFLEYDVVFQLNETLVPSYSREISNLIYNKKFTNPSIAAAAAAAVSAPTTKKSGGIIFKNVSAAKPKPTTNSTSTQNSGLVCGLNPVSTLVSIIKEKYSNYCKVYYDSVGGSKILLKWNDDIETPFAFKPSKSQYTTPMFKKDNLIKINIDEILNEITLIGGKLVKSFKKNENTTTSTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V9R2</accession>
    <name>A0A8J4V9R2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002336</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000063">
        <property type="protein sequence ID" value="KAF2076379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V9R2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="157"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="542" mass="63977" checksum="7BBCC5D17B47FFEF" modified="2022-05-25" version="1">MSTKTQKLTNSRESPVLVQRGGTGYTRYVQQHQRQSRHANETLSQRLGHFYPMIVTLVVCSVLYGIYTHTFQPTPIDSIHINNPIGYELWIKFKDVASHYYELWKQTFTKLKEVGPSLPETGESHAPPEYQLPDIIPSTPPRDGFRNPNFHHPHPVDNTIQREIDRITREMDQHIEEARKKGQTAMQMLEEKRNKILGNLKENIENMKQSGEKLLENKGEQFKDSFDKIADQWENNIQLDKQFIQENFEYAKEKGQDFMDKIQNNIHQHDIKDKIQENVEAIKERGQEFLGHVPSQQQIVNNIHENVDYAKLRGQDILGHLPTQEQLKSNLENVKDYVDEKIPSKEDLYQDYLNMKQKGQTYYGQVKDNLKENLEYVKDNVAEDLKKKVPTDYDWTTLKGVGKDMHREVPINMKSPKVDENIDILEPIREKIPTKDVVLENIDNVLKDFKHKGREFIQSIEDKREEYKEKFSRDPARIKVEIDKVLKEIKESNNPHLLQSLKGRTFETMEDLQELQYKLRDGYREAREHLYRNKRNINNNQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VA29</accession>
    <name>A0A8J4VA29_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CHCH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001981</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000051">
        <property type="protein sequence ID" value="KAF2076724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VA29"/>
    <dbReference type="OrthoDB" id="13601at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="93" mass="11191" checksum="8225D4629589391F" modified="2022-05-25" version="1">MGKGQDRDDNPFKLSPRYRNKIPVIRQTKADCTNFFSEYGICLSQYDFDPSKCSEQLKNIEDCRKETRITRRQSPFNYHLHMIMRNKYRIPIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VBP4</accession>
    <name>A0A8J4VBP4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000271</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000005">
        <property type="protein sequence ID" value="KAF2078404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VBP4"/>
    <dbReference type="OrthoDB" id="17639at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="113" mass="12921" checksum="7535E73290417EF8" modified="2022-05-25" version="1">MSGTLNTTGIYNSNSSVNDGSTPKRRAPEVTMCDEVRKASLRCGESFSKHDCKLIFDAATKCRTLKTKLDEEEVTIKKYLRENVTDQQKVTLETRLNEIRQQKSKQYPVPVEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJ42</accession>
    <name>A0A8J4PJ42_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_010530</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001175">
        <property type="protein sequence ID" value="KAF2068142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJ42"/>
    <dbReference type="OrthoDB" id="16161at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15853">
        <property type="entry name" value="SNARE_Bet1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039899">
        <property type="entry name" value="BET1_SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12791">
        <property type="entry name" value="GOLGI SNARE BET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="12">
        <location>
            <begin position="38"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="62"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2068142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="136" mass="15593" checksum="016ADCB0411B037C" modified="2022-05-25" version="1">MDRSRKKNRNILGFEDENNGGNRFDRNGADSNKVNSEQLFIQQNDQTIEGMNQKANTLKRIVLDIESQVQESNNLLDRLETDLSGARALLSNTMSKLSDLSRSATNTHMLYLIIFVVFIFMLIWFLTTWTSKSNSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PK18</accession>
    <name>A0A8J4PK18_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010268</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001006">
        <property type="protein sequence ID" value="KAF2068407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PK18"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="146" mass="16875" checksum="67A73CFE3F332E92" modified="2022-05-25" version="1" fragment="single">YNNNNNNNNNNNNNNNNNNNNNNNNNNNNINNNINNNSNTSKFTNSLSINDNRSYPLRRVNSTDSVIIDGRMKDLAYNNPHNVFINNNNQNISPLGSPLLNSNRYNNNRIPNNQKFVNKGNTNFPFSYQQQTTNKYSLDNNQEYNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKJ0</accession>
    <name>A0A8J4PKJ0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009127</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000642">
        <property type="protein sequence ID" value="KAF2069550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKJ0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="64"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="168" mass="19237" checksum="7A60F9BA062CEC50" modified="2022-05-25" version="1" fragment="single">PTKYTLQDINPTQPQPQPQPQPQPQPQPQPQPQPQPQPQPQPQPQPQPQPQPQPKPQTLPTSLQPSIFQQSQFQQSVFQPSILQPSQFQQSTFQPSQFQQSASQPSPLQQPPIRLISTKYGPYIIPLDFCIVDYCNNYSGSFHRIPLQKGMLKKSDFLVKEYEVYLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKZ6</accession>
    <name>A0A8J4PKZ6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009917</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000851">
        <property type="protein sequence ID" value="KAF2068765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKZ6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="106" mass="11822" checksum="0C7E82D5ACE69E24" modified="2022-05-25" version="1" fragment="single">IVSNGWIGDIKEIVLSYGLWPLSSTSYPTGPSESVPPFTSRAFSCKQVKIYQTCPQGESRCLPKNTPLTYSQSYTKVFASRIKTFSLTAQQFEQASLHIPMYPLGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLC3</accession>
    <name>A0A8J4PLC3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Leucine-rich repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_009539</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000735">
        <property type="protein sequence ID" value="KAF2069142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLC3"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF46">
        <property type="entry name" value="LEUCINE RICH REPEAT-CONTAINING DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="287"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="289"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2069142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="486" mass="54850" checksum="2D8142A5A4A8B277" modified="2022-05-25" version="1">MVKSQIKDLIKHKHSKLDISGHKLTSIPKEIKKSLDKKTLSILELDLSFNLLKSSVLNEELNQMSNDFSGLTVLSLAANPIGDSFKFISSSIIQWKTVAELNLSGCKLTTLENNTFTPLVSLRKLTLANNYIKSPQDLCFSPCKSTLEELYLNGNTNLTSLPSSLYECKFLEILDLMGCGLETSAFTEKIGLITGLLEMNLGNNKLTRLTNAIGKCKRLCVLNLQDNFLNDLPISIGICIGLGQTSNGINLYGNPWGKNTELASRQSKGPLEIMDYLEKRLNFTNGPVKLDDNDQVDEPKKHLSPQAPVYYHENKPNSPSVENRPPHYNNLNQHRPPEYQEPINIPGPRNDNPNSIDLLKSEFKSTIQTYYEQTTHNQNNITKLIEIGNVLTNMQEKLDTMFHQVGIYKDFSYNSPIPTPKDAKNIQLFKINCLKLEKLAFSSKHTLDYIDSLTTHLKQRRLSSVEFDLKIAHIFSFLSLVFPKLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLI9</accession>
    <name>A0A8J4PLI9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008550</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000536">
        <property type="protein sequence ID" value="KAF2070137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLI9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="298" mass="33554" checksum="57038E5529FDBB6F" modified="2022-05-25" version="1">MDDTITFRQHSLFEIDQQQNVNDDDNSDDKLMEMVLSPINISAQKNRLSSSSSLFNSSTTTTTTGNITTTTRTTRLFFPPMLNISTRQSFLSNHRNSNSVYINKIHINRKRKEQQQKGFMSALSLYDPSKIKTSTTTNNDNNRNIDHDGVDVNNNNNNNNNNLNNQILTGFSNYKTMPSFINSFEGLDLATSYYNVNSTIKTTSSTSASLDPFSNSAILTLTTTTTSTNSGNQQQQDQTDFLSDFENMSLSQKKPARLRFASTSHSHPPSVVKVPLKRLSPLKHFHHKSNNSARIHEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLP2</accession>
    <name>A0A8J4PLP2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009313</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000677">
        <property type="protein sequence ID" value="KAF2069369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLP2"/>
    <dbReference type="OrthoDB" id="24334at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="105"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="721" mass="79975" checksum="F82C3922243D4429" modified="2022-05-25" version="1">MTISTFPTPNNSINIIDSSGSNNVTPLSNSTAIAATTSPASASSSSLFLMIECIYCKSNIPYNNYNDHVYNEVQNTLRLHGIPLFPDHKEQQILNQQLSEINQKVILQDQKIKKLKSKKKRLKEKLKKFTSTSTSSGSVDNTISSNIGSVNINSSNNQITLIESKVVKPSNHGYTIDQIFFRIWRSLSIRNNIKSMVLDDIVIKATPTFISSESMISIPDSAWYSCSSDDRKIGELKNIERIRELELNLTTPLVQKILPKDIRKLVLKCNRLLVFEGIIPDKVEVLHLHGFPQSITSNLFPSCLKVLYLYEGFNHFIFPGALPNTLETLYLFDYSLPLQPNVLPNSITSLSFGPAFTQPISSNVIPSSVVELNFFGPIQLSTTAFLPKLKKLSVHINSQVIKYLLGPNSISELRFIGYLCTQPLKPFDIPPTVTCLNLGDYDHFQRLEPGVLLPTLKSLNLGSKLTKEPVKLILSSLTTLIMPSLKTIMGPIPSTVTYLDISGCQDPIKPNFIPNSVKTLIFNHSRDDVCIGAIPSSVTNLIFDSTFASHLPRGAIPDTVTSLRLGGANYMETIGSGVIPSSVKTLFLHTGHKQQPFILIPNSVTDLEIVDTYQMKPRFLPNSIQSLTVSNQNHPFLFGSIPPSVHTLFLVNNYSVIILPCEIPETVTKLKIKSVNYSVIEGSIPHSIKILDIDSPILSNRHNIPSTINTFIKSGTFDKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLR1</accession>
    <name>A0A8J4PLR1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="10">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_009283</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="10"/>
    <dbReference type="EMBL" id="AJWJ01000670">
        <property type="protein sequence ID" value="KAF2069399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="15304at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19673">
        <property type="entry name" value="UBR-box_UBR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.30:FF:000002">
        <property type="entry name" value="Putative e3 ubiquitin-protein ligase ubr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2670">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042065">
        <property type="entry name" value="E3_ELL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044046">
        <property type="entry name" value="E3_ligase_UBR-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055194">
        <property type="entry name" value="UBR1-like_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF49">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18995">
        <property type="entry name" value="PRT6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22960">
        <property type="entry name" value="WHD_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBR-type" evidence="12">
        <location>
            <begin position="91"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="9">
        <location>
            <begin position="91"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="970"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1019"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1024"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1036"/>
            <end position="1046"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2069399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1625" mass="186322" checksum="F5CD6F77B6BF948E" modified="2022-05-25" version="1">MNNISIFNEIYQYLYNSNNNNNNSNSDSNKHIKNFDSIWLFFNQLLSIKPPNRILDTLVECLTVNNNDSNSSQLKTFDSIKQYYSDVVIKSVCGKVWNNSLVYKCLDCQVDDNCSICLDCFEEDKHIGHRFRFIKSTRGCCDCGDSSAWRKEGSCRKHSGLANVQDNDDNKNSSSSSNNNNRKPLPINTSLIFIDVILELVLIELIESSTRSRAAPPPTNAPITIPPSASHKTMITQRLIKIVNQFIEILGDTFTMLCANKILLKSKTSKNLLKYLLRPRDIEMENFVIALFICHPLRDELAMCLASNFECLENSNLSVQLFTVDAIAQQIVEQQTLTPIVECFLSHVRENKDNETDLVKSGGFLSKGYFKSLVAMSYIIRTKPSFLWLIDHPNQMLEALEALQLCQGSSENIRYVTSYISNDDFWIDNYYLEYFVGRYLSNLFFGKLLYKHAALVKETSIAVYKHYLLNSILQFVDANNGGIKWKRGVRDKLVPEYRIQKQPISFHLPLHRSLCSVLYFYSLHYGKTPVQTLVLPDSTLAPDQLACLLAEHVMRIFVLVFMHDAKTWMRNGESINKQAYNYKMQQEFFATDLHLVQYLLHIYVDPTLFYSQILDRCELTCWLVLDGHVPDSVSVADQNSITSGTRPPHRPESDRINAVVTENLLELIVHLVCERTKLADMSTEQILRRDLIHRLAVGPQTFSEILGNLSEDLENDPIVERVLLTVSDFHTATESVNGKYHLKPECWTEWTYFYPYYSRIDQQKSEENYILYFKKLNNQKEPIRPPLVTLAPTLPIFNQDKGIERIIHNDILHSILYTVIFNACSGSVRVNDRLLTTALYLVSLCLDTFDPSLIPSIANEKIKNDQFDFGFTHNIVFNFYHPVKESQVIFFLQASVHSLSSLLNHMLGVLRFDTYHPIIRYILNHNVLKQHKDVKGILSTSSSSTSLHLAQGESISSTNSSIATLITSTTTTTTTTTESTTANSSMSTSPDSSLLKEAFRKKQMEILKKFTEQQKSFLIRNSNGDEDDQDDNDIDQDIKHSTKNKDQYQPVNNNKETVLKKDYGECIVCRENMNTVNGVTNKPFGYIGFIQPHSLLSCNPLANNCQENQTNDLLFSDINASDGSFINFCSHLVHLCCHQLLVSSGVADLKYGFQCPCCRRQSNVIIKTTDIVFNIPSSSSSSSTSLSDFILDLKSNNLNLSTTMNQNNQNQNQLQQQQQLDMIYSHLAEFEINNPSRYLHIWNLVCSSLSLFELSKRKDNVKQVPVLVNSRESFQNLIKYCISIISGGTAIDKQQAIIDFKKILNTIFNQQTSQTFLSLDMFGLFVKFYLSIESINNKNNNISFNYYVSIFVIGNLIQSILVVNDNIKSYTDLYNYLKSKLIDNNDNQFITFSRNLEKQVKDLCIPFLKRILLFKTCIDSNIDIIGIDQDQDEYEILIKYIFNSSNIVLFNDDLMEIIKQWIISFSIGNLDNNNNHNKLPLRSEKEFELKVDLPLDYLKLLVNNTKGKCDSCSSLEEDISSPIRLVCLLCGLGLCHFHFTMHHSQHRIGVYMVIPESYIFIQMNKRSRRWGSIYLDQHGEEDLYFQRGNPLLLNGKRLDKLKQDILLLNNIQYLPYINIPPSRVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLZ0</accession>
    <name>A0A8J4PLZ0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_009205</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000655">
        <property type="protein sequence ID" value="KAF2069472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLZ0"/>
    <dbReference type="OrthoDB" id="15258at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20617">
        <property type="entry name" value="CYP1_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000068">
        <property type="entry name" value="Probable cytochrome P450 508A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF417">
        <property type="entry name" value="CYTOCHROME P450 508B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12 13" id="KW-0349">Heme</keyword>
    <keyword evidence="9 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="432"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2069472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="486" mass="56164" checksum="4A4F7D40EF62AAE4" modified="2022-05-25" version="1">MIQTILIIFLLYIVYDFIYRNKKYTKNDPPGPIAYPLVGDLPRLSKLPHINLLKLHQKYGDVIKVWMGDFYTIIINDPVVVREIFVKHFDNFTDRSHPLPTKLLSNNFRNLISGDYVYWKRNRDLVSHAFTNTKLRSVSSLIELEANRFIQMMNDDYVATGQPFCPRLFTKKYTLSVTLQYIFSGTLPLSPNDKNVDEKMVVLAKEMDNIFLYIGRIRAERFISSYAWVCYMLKKYVFAVQNTTIVQCMKDIMLDHIKKLDPENPKDLLDYILCNIDIKDPEEIKIPLMIGTDLMVAGTETTASAIEFFFMVMVNYPQFQELAHTELAQVVGKGNKVTLAHKKSTPYFMALLKEVIRKYPIAPLSLARSGKQDILIADKYFIPKDVQLFINIHALSNSDKYWDKPNEFNPDRFLNNNHSDVFLPFGVGPRNCIGSNLAMDEYYIFCANMILNFTLSSVDGKPIDETLSSSLSLILQNEFSVLLKHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM92</accession>
    <name>A0A8J4PM92_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009104</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000637">
        <property type="protein sequence ID" value="KAF2069578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM92"/>
    <dbReference type="OrthoDB" id="24485at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="576" mass="68457" checksum="B2638824BD7DE922" modified="2022-05-25" version="1">MARTIIQQPVDHSENKHFYQSFNSNHDISQHVVEQQREDDDHCELDSTTEIQQHDNRNKKLKLENNQNSLFVDVFKNITIRTHIFSFIHRDLVPFYKYSDIHSLKWILNYGSTQLFVDKVKRNEYLSDILYLSNRLNTFKSINDIEFYTIFNHRYRVYIYDIAMINVKDFLDTILNNNNNNKTLPVGSKRKEMETESRTKPLEENVKDINTSYINDLSVEELVQEYKFNKRREFNNFKRALESKHFQLAKYVLDHWLNQLQFFEQSHSPDSMINIGDKDTIEYLCRMDTKAKFHLGIGLQNIFSRPDFQQLVQLLEQSGMIDKSNTYHMDYFKHKRNLSYFIENGYEHSPQIINTIGLGSKEQFSCLSMFLDAGLDPDGDIISLINQVSIHREYQLYNELFRRYPATNDLLDVYPSILRRYNAYKAKYNALFTPNYQQYALLSEIPTRIKRIGQLGNNLNIKSLFQNHKMQLLSPYMEYLDQVRAQQTHYTDNVEDDFYIQSESILTVLFYYLVQSNHLHILKMMVQECPDIFQAFKNQDGFTNFDLVTSFSIKNKSFEILDFLQTLCGSPKEKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMA4</accession>
    <name>A0A8J4PMA4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Ergosterol biosynthetic protein 28</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_009050</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERG28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000625">
        <property type="protein sequence ID" value="KAF2069633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMA4"/>
    <dbReference type="OrthoDB" id="6485510at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005352">
        <property type="entry name" value="Erg28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15451:SF19">
        <property type="entry name" value="ERGOSTEROL BIOSYNTHETIC PROTEIN 28 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15451">
        <property type="entry name" value="ERGOSTEROL BIOSYNTHETIC PROTEIN 28-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03694">
        <property type="entry name" value="Erg28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="8" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="11" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035281561" description="Ergosterol biosynthetic protein 28" evidence="14">
        <location>
            <begin position="26"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="75"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2069633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="14558" checksum="E14772E6F13E58E5" modified="2022-05-25" version="1">MFGLQNIQVWLLTVAGLRLLGASQAFFNDSRLKSNVYSGDPKSVTRLQARLFGNWTMMSCTLCLATAYSPYNRELFFVTWMSFFYAFMHFVTETFIFKTSKVKDAIPPFIVASTSMIWMGYLLLNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMZ5</accession>
    <name>A0A8J4PMZ5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008559</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000539">
        <property type="protein sequence ID" value="KAF2070118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMZ5"/>
    <dbReference type="OrthoDB" id="60283at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550:SF10">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="743" mass="88013" checksum="9D7A49CCA67E6EC9" modified="2022-05-25" version="1">MQQLFKNVFNNVYIRKKIFNFKKRGGLGYDDIVDVDWMVDNEYWALLREKLERGDPLRLVSHLKILEIKPFDLFVLAFTRFRHHFSVHEPVLNTLVAYHGVTDAYYYLEQMGYTGDLYYTLKNAWYHENYQFIKDLLDSNREDKEKMQIDLNLTQAVAEKNVEMLKYLFEYVKQCDKDSDGAPVPKKGHLFIYKANTRARTQLLAQLYTMVCVDGSVEVFDTLYQYFPIYFTPDWFKRVTKENELLLIHIFNKCQEEIKGSWISKRYNILFHMALGCVKEQVYCREFYQFIYAKYQECLFNEDDFGKIQDFIARLAFKHQDTELYDLFKSENCTLDSIAISEVKDGFKDLDRVSELYQWSLKISKECVLNAVAQDRDDIFPFLFKYINTKLLGGVKSNLLQVILDRKAVKVLGYLVEKKIILKGSVTKWDQWNLDNNLAFIKTYVESYPLKNIDEYMFALKRCASQGYLKTFQYLFNQFNQSTLRTLENQSLNTDQLYGEAVAHCQNEIIRFLAIDEKIPCLDTTIFYSAISVESLEIFNEAGHSNITTLANEDLLISKNIPCFHVFKYIIDGAKIGTDHSMYLQSLLYQIVQKNKVRHFKYLLDTYGNESIDINIHRLIIQNGNLEILKYFDQYILNNRSFIVETYERSLEIAIKNNQLDILMYYLNNNTYNNNNNNNNSKKIIINNTILDLVNQKKMAMLEYLLKTEFKESLMFLKKEDLEIDDEEYYQNFLKQLIENLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNA2</accession>
    <name>A0A8J4PNA2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000528">
        <property type="protein sequence ID" value="KAF2070190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNA2"/>
    <dbReference type="OrthoDB" id="19026at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13673">
        <property type="entry name" value="Acetyltransf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="230"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="315" mass="35305" checksum="8C82973C7B5D9205" modified="2022-05-25" version="1">MSTSIIKLLKDCFSLKDTAEILNQAFADYVIPFTVTELSLQQKITSEDVMVEHSIGLFNKDTGALEGFLLHAANDHQHPTQLYNSVTGIIPAARGRAVERCYTMMRPHFESLGIQSVVLEAISSNDRAVRAYEKCGFVTKRLLHCYKGEISKDALARALASKPAPDIRVQKVSQLSPQLYEFMLAPPQSADDKTPVMVPAWSSTTISCKREFDIGVNAIWTATTTDENGKDIIVGYVSMNASNCRLRQLYVLPQFRNKNIATTLINTVVSNFDASSNYNIIIDETFDNLTNFFTKRLGLSFFVTLKEMFLEYNKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNI2</accession>
    <name>A0A8J4PNI2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA exonuclease 1 homolog-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_007556</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000410">
        <property type="protein sequence ID" value="KAF2071127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNI2"/>
    <dbReference type="OrthoDB" id="21204at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031736">
        <property type="entry name" value="REXO1-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15870">
        <property type="entry name" value="EloA-BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA exonuclease 1 homolog-like" evidence="3">
        <location>
            <begin position="266"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="166"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="101"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="170"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="197"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2071127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="360" mass="41115" checksum="FCB7F1A2FDF34209" modified="2022-05-25" version="1">MFKTNAFKDIECPYDNCHLNSICFFNHNLRSNSINNNNSNINNFNSNSNIASYNQQQQQQQQPPISNRLSLQQQHQLNRLQQQQQQQQQQFQQLQNFNEIEDNDGEDNEDINGYDPGSTKIDIPDEMIDDDKIVSNPCVSSVPSKPPTSLIVSEIDQKIFTKKKRVDMKSNIQQQQQHPHQQQHNHHSQPIVSNTDATTTTTTSSSATTPTPTPTLQKLKTSIWNPTLKNILSKTPSKEILDLIAQEDIYNPIKWNSLGIEPAQLPTIITESRTKVPRSVRQIMLFHIVERCVAVVPDKRKAIEEATAIEESIYADALSKNVYLNLISMELPKLLAQLEEVYSFKPTNLNMTTQPDHEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ24</accession>
    <name>A0A8J4PQ24_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007678</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000423">
        <property type="protein sequence ID" value="KAF2071002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ24"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47941">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="233"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="268"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="424" mass="48091" checksum="16E9F0EDAB789F5E" modified="2022-05-25" version="1">MQSISKIGINFIRSKKCLGIYNSISNSSRNRFYCTNSSNSNKDNKDKNNIDDIPEADIVDEKKYIIHKTTTTTTSSIQINNDEESVKIIKDQELENDSSVELKEQLLNYADGLEKSPINPFEYSIKTVDDALQLPDIKPINIESTYAVGAMHEKLKESMPFLKELVEKGHVENAFSLFQVMERYYVPVSTECYDLLRQKIEAAMVEVALFNEAIKLETINPGSVDPDRLYIPTRETLNTVLNYYTETDKLVEAFRLLLTMKTVGVMPNQHTYRSIINAALRYEDIDVALIAFENMRSDGIQLEEQSYERLFEACCNSVHYDGASDLYYELINNFHIPTSNRLAFLTTLGITRLMNWFGLPKSVRSGRGWVTSLQISPSRFIFPPKNENAKLLSPNVPKSILLKLTSSGGHNNNTNSLGGLLPFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQB2</accession>
    <name>A0A8J4PQB2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cell cycle control protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_007777</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the CDC50/LEM3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000433">
        <property type="protein sequence ID" value="KAF2070911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQB2"/>
    <dbReference type="OrthoDB" id="340608at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005045">
        <property type="entry name" value="CDC50/LEM3_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926:SF0">
        <property type="entry name" value="CDC50, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03381">
        <property type="entry name" value="CDC50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015840">
        <property type="entry name" value="DUF284_TM_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="28"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="266"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2070911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="306" mass="35025" checksum="7A1AF3EF9A3FFAC9" modified="2022-05-25" version="1">MSSKDPQHSKRPANTAFKQQRLKAWEPILTPAPVIISFIIIGVIFIPIGAVMINASNSVIEHTVRYDDLCGKEPNCTTTIEVPKTMQAPVYFYYRLENFYQNHRSYVKSRNDNQLRGEIVSAYKDLQDCEPFKSLDDSHDPQNFYLPCGLIARSMFNDTFVLKQGTPIPLKKEGIAWTSDKEKKFKNPPANAVGIRIIPDFEDEDFIVWMRTAGLPDFKKLYRIIEQDLQGTYTVDIQNNYNVSSFSGKKYIVLSTTSWIGGKNPFLGYAYIIVGIICFVQGIVFLVKHKVSPRKLGDTKYLEWNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQW3</accession>
    <name>A0A8J4PQW3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008111</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000474">
        <property type="protein sequence ID" value="KAF2070564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQW3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035288683" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="88" mass="10143" checksum="4B2012B7CA914FDB" modified="2022-05-25" version="1">MIHLVVGVVLLVILLNIVLSNEIGIPHDEYLKEQSGVVSMKDQQEAEYKDYNIITWTLCNIFWGVLSTLYILLSSIFGFHVEFKEQDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQX3</accession>
    <name>A0A8J4PQX3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">AAA ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_007105</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000361">
        <property type="protein sequence ID" value="KAF2071572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQX3"/>
    <dbReference type="OrthoDB" id="47330at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19499">
        <property type="entry name" value="RecA-like_ClpB_Hsp104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000017">
        <property type="entry name" value="ATP-dependent chaperone ClpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000120">
        <property type="entry name" value="ATP-dependent chaperone ClpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000025">
        <property type="entry name" value="ATP-dependent Clp protease subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000010">
        <property type="entry name" value="Chaperone clpB 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050130">
        <property type="entry name" value="ClpA_ClpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638:SF176">
        <property type="entry name" value="HEAT SHOCK PROTEIN 78, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="143"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="540"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="domain" description="Clp ATPase C-terminal" evidence="10">
        <location>
            <begin position="709"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="73"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="357"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2071572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="802" mass="89378" checksum="DF0FFF861D7C813F" modified="2022-05-25" version="1">MNRLSKISNSVKQIIGPKYQTRYFSSPSPSVGTIVGKSNNILLNNSIGGNKNTLSSSSLLFNQQYDLNNKLNKRFYQGGQGGGQQPPQPPQEPWVSPDNVPAGEALKKYTKDLTEIAKNGKLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGDVPESIKNKRVLSLDLAAIVAGAKFRGDFEERMKSILKDVAHFKGEVILFIDELHTLVGAGAVEGGMDASNMLKPQLARGDLHCVGATTTSEYRKYIEKDPALARRFQSVMVQEPSVEDSISILRGLKERYEVHHGVRINDSALVAACVNSQRYITDRFLPDKAIDLIDEAASRLRLQQESKPENIENLNRQIIVMKIEIEALKKEKDQLSKDRRAKLENELKEKEKECQELTAVWKKEKEEVEKTKGIAEKLDNAKIELQKAQRRGDLGRAGELAYSIIPTLEKQLPKEGHEHNFSMISDSVTAKDVALVISKATGIPVHSLLMGEKEKLLKMENTLGDSVVGQPEAVTAVSNAVRISRAGLHSHNRPLGSFLFLGPTGVGKTQLCRSLAEFMFDSPNALVRIDMSEYMEKFSVSRLIGAPPGYVGYEEGGTLTEAVRRRPYSLVLFDEFEKAHREVSNLLLQILDDGHITDSQGRKIDFRNTMIILTSNLGAEVLANLPDETPSSTAREEVMGVVRSRFPPEFLNRIDDIILFNRLSRKDMDKIVDIQLNDLRKLLTEKQIALHVKEDGLNWLADQGYDPIYGARPLKRVIQKYLLNPMAKLILEGEIKENQTVTVSVDDKSQLSITSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQX4</accession>
    <name>A0A8J4PQX4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_007478</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000401">
        <property type="protein sequence ID" value="KAF2071210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQX4"/>
    <dbReference type="OrthoDB" id="18578at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056840">
        <property type="entry name" value="HEAT_IPO9_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF9">
        <property type="entry name" value="IMPORTIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25018">
        <property type="entry name" value="HEAT_IPO9_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="6">
        <location>
            <begin position="31"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="946"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="946"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1017"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2071210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1017" mass="115831" checksum="73F14E4D42A2D353" modified="2022-05-25" version="1" fragment="single">MTTELSAFQINKENVLQFLNGLLDPNLNKQYESHLDVYCFNQNGFGLTLAQIGIDTSINVDIRQFSFIILKKFVQERWEGKQIPSDAEKAEIRHIISPLLADPSPKIRTAIAMCIGKIGVYDWPAKWPSLLDDLMKCINNPGNMNLFSGAMRCLEILCCDESDHENIAQIMISSFSVLNALLKRIDLEPLVHIKITQVFKKIAIYTTTNMDVNKTLFKTLLPFINEWGDIFALHLEAKIDPKFINEMFTIQANLIETISMLYNDFQSSVSPFIERLFSSVWALFKSSYPYYEQIEIFGEDHTPKTLEEGVFGIDKLVSSLLEFLTLVINKKKYKGIFEPIIRDIIYQVILYMKMPNDWLDLWETDINQFLDQEDDHSYNPRVISVELLTSILASYPAARGAMVELVKFLFVKASEKQNPNWWRYREVCLFILTTYANEFIAIAKKNPQLFDATQLLQNQLPLDFAASDKQEDEEYTGILKGRSLTCAGAYGRIVDSSISVQYLNQAVQVISSSTSPLPLKLAAIKSMGTLIESLPLEMVNTGIPKAIQVTCSFLSQLSEDSLLLVLDALLLFTKIDRDITGSMESVLTPSLTAVWINFANDQLICETINDIFRVMASCPKSYPGILKNLLPTLNIILCAYDKSLYIGIADSTVDLLGIVVSKLDSSIIDEFLLRQLLDPLLNILVSIYNDESSRKLARAALLSVIPFIYKLSTQVLVEYKPVLENKTLPPQSSLADCLFFIFQSYIVQSELISGNAIPILKALISKHTSIINNHLANINNLLIDRLANCKLSTTKEALCVVFARLFLIYKQNALDYLFTVPSPDPEFASALEFILSEWNKSHPDIQTYLDTNISLLALVSLFDYSTDKRFQSIQVHSTPITNEKSSRNINKKLKEKWTKCNLYEKATVNIYESYKLMLLDEKEAEAERKKKESAANGEYDDDLYEEVEVEEEEEEEEEYIPEEQGEGFAPAAEYQHILDSIDFTNNENDDDTDNFEVDTDILEIFDKEDQYYNVKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQY4</accession>
    <name>A0A8J4PQY4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_006715</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000321">
        <property type="protein sequence ID" value="KAF2071978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQY4"/>
    <dbReference type="OrthoDB" id="19324at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20617">
        <property type="entry name" value="CYP1_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24303:SF31">
        <property type="entry name" value="CYTOCHROME P450 307A1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24303">
        <property type="entry name" value="HEME-BINDING MONOOXYGENASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12 13" id="KW-0349">Heme</keyword>
    <keyword evidence="9 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="377"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2071978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="432" mass="50468" checksum="CB79D0EE6201CA15" modified="2022-05-25" version="1">MKITEYYQGRPFRLWMGDIHSIIISDPETIREIFVKHCDNFINRIEMPSISVVSSDYLSLSASSQPHWEKVKHVMIQAFTQLYKTKAKEISVVKVEKFIHVLKLYTKSGECFTPRRYCQTFVINIMLKFVVGKSINYTEVREDRELLDRLIKPMEDVFTHLGGHHLGDFVSFLQPLYLFYLENFNDPAADLKKYIDEEILKHQKTYDPDNHRDVLDLLLSNLDHVNGDLKTIRALLQDFLNSGTETTSTTLEWFFLAMANNPDVQEKAYQELVKIADKETMLITYCQRQYTPYTNALIKEVMRKYPVLPIGLLRNCSNDITINGTHIPKDTIVFQNLHYLFKSDRYWDQPNEFKPERFLEDNHSDIFHPFGVGPRLCVGQSLANDILYMGIANVLLNFKIQSINAKQVDETELFTLTVHPNLFQVSLKSRLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRD6</accession>
    <name>A0A8J4PRD6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006417</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000295">
        <property type="protein sequence ID" value="KAF2072263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRD6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="251" mass="28217" checksum="26B3B35F8C28F4F0" modified="2022-05-25" version="1">MNQINNFKKKLKENKRNDSTTAAAVVDSNINNNVLNNNINDGKKSNSDNRVLDKNETGINGDNLFPTSGSVAKGHSLTTPLKSKNSLSLGLLGKGRKSKVISSNSMTFSEDHFFSTENLPTLNEQDSLKKPLQELNLSKRNINNNSNSEKVQKQIQPRYMKNLTDLQEEAKDEFMNLKVENEKQITQALVDSSNGNLTNDKVNQLIETAKLLVDKNETTLENMYAQLNFYPENINTSENLVEIINKLNEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS76</accession>
    <name>A0A8J4PS76_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Electron transfer flavoprotein-ubiquinone oxidoreductase</fullName>
            <shortName evidence="18">ETF-QO</shortName>
            <ecNumber evidence="18">1.5.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">CYY_010128</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Accepts electrons from ETF and reduces ubiquinone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 18">
            <text>a ubiquinone + reduced [electron-transfer flavoprotein] = a ubiquinol + oxidized [electron-transfer flavoprotein] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24052"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.5.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="18">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ETF-QO/FixC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.5.1" evidence="18"/>
    <dbReference type="EMBL" id="AJWJ01000936">
        <property type="protein sequence ID" value="KAF2068546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS76"/>
    <dbReference type="OrthoDB" id="437331at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004174">
        <property type="term" value="F:electron-transferring-flavoprotein dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.20:FF:000015">
        <property type="entry name" value="Electron transfer flavoprotein-ubiquinone oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040156">
        <property type="entry name" value="ETF-QO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049398">
        <property type="entry name" value="ETF-QO/FixC_UQ-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007859">
        <property type="entry name" value="ETF-QO/FixX_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10617">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN-UBIQUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10617:SF107">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN-UBIQUINONE OXIDOREDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21162">
        <property type="entry name" value="ETFQO_UQ-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05187">
        <property type="entry name" value="Fer4_ETF_QO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 18" id="KW-0274">FAD</keyword>
    <keyword evidence="5 18" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12 18" id="KW-0408">Iron</keyword>
    <keyword evidence="13 18" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 18" id="KW-0813">Transport</keyword>
    <keyword evidence="14 18" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="ETF-QO/FixC ubiquinone-binding" evidence="20">
        <location>
            <begin position="256"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="ETF-QO/FixX C-terminal" evidence="19">
        <location>
            <begin position="493"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00052682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU366068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF05187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF21162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAF2068546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="601" mass="66313" checksum="4630304BAF69AADE" modified="2022-05-25" version="1">MFRIGNYINRGTTLFKCSTKTTSTATTILSNNSNFLKSIRFYSTREELPERDSDQFDVVIVGAGPSGLSAAIRLKQLSQESGKDLRVCIVEKGAEVGAHILSGAVLDPKALNELIPDWKEKGAPLNTPVATDKFYFLTEKSSIRLPTPRLMHNEGNYIISLGNFTKWLANQAEELGVEIYPGFAASEVLYDEKGAVRGIATSDMGVAKDGSLTSNFTRGMELNAKLTLFAEGARGSLTKTLFKKFNLRDGVEPQTFGLGIKELWQIKPEKHNLGLVIHTLGHPLELETYGGSFIYHLEDNKVALGLVVGLDYKNPYLNPYQEFQKLKNHPLVRDMLEGGNCISYGARTINEGGLQSIPKLVFPGGALIGCTAGFVNVPKIKGNHNAMKTGMLAAESAFEQLVEAQEKQDAEPLVVDSYPENLKKSWVWEDLNEVKNIRPAFHWGFIPGMIYGALEMYILRGRAPWTLSHGKPDNERLEPAASSKKITYPKPDGIVTFDLMTSVSRSGTNHEENQPPHLKVRDSDVAKKVNYDVYGKPESRFCPAGVYEWTEPEEGDTTTKPKLVVNAQNCLHCKTCDIKDPTQNIDYTVPEGGGGPAYNGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSP4</accession>
    <name>A0A8J4PSP4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MMS19 nucleotide excision repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_006198</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA metabolism and genomic integrity. In the CIA complex, MMS19 acts as an adapter between early-acting CIA components and a subset of cellular target iron-sulfur proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the MET18/MMS19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000275">
        <property type="protein sequence ID" value="KAF2072489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSP4"/>
    <dbReference type="OrthoDB" id="342900at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039920">
        <property type="entry name" value="MMS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024687">
        <property type="entry name" value="MMS19_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029240">
        <property type="entry name" value="MMS19_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12891">
        <property type="entry name" value="DNA REPAIR/TRANSCRIPTION PROTEIN MET18/MMS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12891:SF0">
        <property type="entry name" value="MMS19 NUCLEOTIDE EXCISION REPAIR PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12460">
        <property type="entry name" value="MMS19_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14500">
        <property type="entry name" value="MMS19_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="MMS19 N-terminal" evidence="7">
        <location>
            <begin position="46"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="MMS19 C-terminal" evidence="6">
        <location>
            <begin position="558"/>
            <end position="1009"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2072489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1056" mass="119553" checksum="7046DE68C1D46579" modified="2022-05-25" version="1">MNQNELLQLTEAYCNNNNEDSVRSGALNHILLSMSSKKIEIHSLVLSLGDYLKSRDSILRARGTLLLSEVLCRLPDLPLNQDQLHFLSAFFSDRLQDYSCAAEVIKGLTGLLTNHTLEYPNNQKILRGIFTEVHPSTLPHSHRKMVLQIINIMFDKCLPEIQELKSDFMAGYLQFIDGEKDPRNILYSFQLLPKVIKNIPEYAKFTESLFEILSCYYPISFNPKPNDPNSISKDDLSNALLSCFVCTPLLAQHCIPFLIDKICSNLVETKIESLHTLVYCCKNYGPYAITPYLEDIWSTLRTNILTHKKENVIEESKKTIFQLTRIFTRDETVLNSFLEIIIKECLHHIKSSQDSKISIYCASILFQTVCASPISSKIVLKTILPNLFSFFKDISIAAGGNESGTLKINEQVSIIGLLNDIIKANMISFQQQEKQYDGENPLTPYHDTLYQLLSDSLLNSSSAMRSASIECLSNLYISKKSNDSFNFLLDREKRDTILKVLVSLINSTDDTLRQKSLDSIYNIATNEETTILNGYVVPTLLQMINSVSADNTRELQYYLDAFGKLCSHQLLLDTVIPQIQGLIQHNIKESWSTKEDFEKSKLVLLSISKILDSSTNETSMTICCQSILFPLIKGLFKQIIYIATAINNQQQQQQHLDYFNIICTPCLDTIRSIIQNTSRSSQENAITDVIDVYLNGNLSLLFPDNVQEFKPFASATPLIYKYLIPIFTCIISQSKTDLSSNQSLKQALFDTSLDLNVPDHISIECVKSYASILNKQSPNEITIEYFKSNIIAPILSNESSLPLKLRLIVLLTWCTKALLTNGHPINIELATFISSLISNPDSDISKLACKSFYTLLCDSDILNDKSGSVIKILYQQKFFTLMFSILLNSFKENKDNQMLSSKYLIAISNLLEHVSKDMLLGELDEILPIVMHSLKSFVVTNNNNEDTNESQQSYLLLESSLQTLLMLIKETPTSFTSYLDSLVPSLIKLSVSAPNQKSRKSSLEILYFISVSIPYHNIYPFRGKVIAGIVPALDDKKRLVRRDASKCRNSWYILSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSQ7</accession>
    <name>A0A8J4PSQ7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">U3 small nucleolar RNA-associated protein 13 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_004844</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000179">
        <property type="protein sequence ID" value="KAF2073848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSQ7"/>
    <dbReference type="OrthoDB" id="5414888at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000230">
        <property type="entry name" value="Transducin beta-like protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001665">
        <property type="entry name" value="U3 small nucleolar RNA-associated protein 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013934">
        <property type="entry name" value="Utp13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854">
        <property type="entry name" value="TRANSDUCIN BETA-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854:SF15">
        <property type="entry name" value="TRANSDUCIN BETA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08625">
        <property type="entry name" value="Utp13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="131"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="179"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="214"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="424"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="466"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="520"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="562"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="604"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="646"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 13 C-terminal" evidence="7">
        <location>
            <begin position="699"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2073848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="874" mass="98561" checksum="1885C2CED390A317" modified="2022-05-25" version="1">MSQTMNYRVVECEIPSDYEDEEQDQQPQKEVEKKPPQKRLKSLFKPEFELGSLYTGGSVVISSDSKFIAAQSGDTINIIDLELGSVISKIQNDAKITSFALSPNNEELLVGCVNLHMKQYKISDQSLIKVWRGHEGPILSIDYHVSGGLAVTGSSDRTIKVWDIEKGYCTHNFQEPDVVSNVRFHPKKLEIISVCNDLNIRIWDLMTKQCSLVLSNHLSLISGITFSNSGNELISAGRDKVLNVWDMKSTSPKKTIPIYQELNGIITLSNTHYSTLPSNSVAKLEKIKKKLLDTPQWADKVKRDDLTVVIGGEDVIRAWCVETGECIWNDQNIEFKKKDDKTQNDTPLFTFTALINNNEKMIGITSEHNLIIYNLKDLSRVGELIGYNDEVVDIKYINPENIVVATNSNEIKQYDLKTKRAQVLRGHQDLVLSVDVSNDGQFMLSGSRDKSAMLWDLNSKKPIMSLNGHTSSVTCVAMSKKQNNFAITASEDRTIKLWKLAPNESVKSGKVQKASAVLTKIAHEKDINAISIAPNDKIFATASQDSYVKIWSVNNFELLGTIKAHRRGVWGVEFSPVDQCILTCSADGTIKIWSLADFSCIKTFEGHKGSVLKASFISFGMQIVSISAEGLIKLWNIKTNECVNTFEGHDSKIWGLAVNKDQEHFITGGSDSRVIVWKDHTLIEEEREKQEEEKNILYKQNLETALRNRDYLSALRLAFILNQPQQTLSIFKQMYSDTGNDALIRENVVQLGNNEVVKCLQFIRDWNTNSKFVTISQMVLNSIITSRTPEEFSQLSTKDIPRILESLIPYSDRHFQRIDKMLQKTYLIDYTISSINPAANTLLVDELNQPVKIKSNNQKLTSKKRKAGSAQNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSY9</accession>
    <name>A0A8J4PSY9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000257">
        <property type="protein sequence ID" value="KAF2072696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSY9"/>
    <dbReference type="OrthoDB" id="19429at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="328" mass="37109" checksum="B255C9261C0D8F0C" modified="2022-05-25" version="1">MANSAEPESCYCDPSVARGFDCHEAGCRAYASVYFIFFLAITIESGRRLWFLRKNYKTAPFISLSQLCGGGLFFTIRHILLLAKVQEKFSLGFFLLFGVAFIISSYLWILIVWCNIIIQVNFSKGVQRVLPWVRYLIVFLNIALFIGFIIGVAIYWDWRATNIWIAVYVAIEAVLFFSFGYTIWKDYKDIGNISANGKMAEQTYKKVKKITKLSVYAIVSSILVAIFLWIGSMWKFKSDSQKVLYLFIQRFTLILMMVSMLVCLTLPSDDPVANDGSPSPTGSSKFNTKMKDLAFSKGDDDDDEEKGQVEEIGQSNSSVENISGQSNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT35</accession>
    <name>A0A8J4PT35_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PAP-associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_005909</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000248">
        <property type="protein sequence ID" value="KAF2072786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT35"/>
    <dbReference type="OrthoDB" id="20488at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002058">
        <property type="entry name" value="PAP_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271">
        <property type="entry name" value="POLY A POLYMERASE CID PAP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271:SF40">
        <property type="entry name" value="POLY(A) RNA POLYMERASE GLD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03828">
        <property type="entry name" value="PAP_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAP-associated" evidence="7">
        <location>
            <begin position="1103"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="392"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="395"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="416"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="438"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="491"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="512"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="525"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="544"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="592"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="610"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="628"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2072786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1230" mass="140863" checksum="5448D2B3217F9DBC" modified="2022-05-25" version="1">MLSQSLKDFVDSVTNYTTRDDWLKYELYTSRLWKKYINTNVVQFEFDNPKHFDDFFAELKEEFRSSMRSCVLRMVAGKSNLDENEATTLKIKYTNSLLDLIDERAALEREKFLTNRNHYVFVVYDLQFAVSFREQIKSKLQAYSLLKSLNSTPAYGSSPITSLSSSLSTNGTDTLSDSTIIQKPSLEDDILMDNSNIKSQSFRADEILYNIHTFDKYFRDVVNKLTTYIDNQDWKNRKDLDLGTESVYVYFDNRKEFDLFFKQLSDICFDTLSNIRENLKRMIQDLKPDGTKKNTHPEYQSLFSILADHQNFVKDFFFNDEKRELRQFTLDPIKYSYNIYSNRRFKFLQKDAYQRLLYKPKISTLLKDCQKVIEEKKRKAVDSLLNNNTKKSKFETSANSSSFSGNGSTTPPRITSSSNSNSSISSSSSNSNGNSDSTTPTLDKSNGGSGSTTPRPTNSYTSANTSNPTKPTTSVAVPLENKSNIDNKGNQQPPQQVQPQQSQPKPAPQEPEQPQTQTQSQSQPKPTPQQPSPPQQQQAKQLSPIETKQEQISPPLNGTIATLPATDSSPNPPVAQALPKIPPIKLEISKPAKSNTKSLSLPTHKPPPTTASYSTPTSLPTHTPPPTTAAATSTTIPTHTTTPTTIPTEKTISTLPFPTESSIATTTTVNTTSTILEKQQQQQQQQQQQQQQQQQTTSNIINDNKQLLDNNNNNPSQYLSINNNSNIKSKILDNLNLSSRKSIENSNNNNMIIKHHQQQRESELEIINQQENDQVQELLSYIEIKLNDKKLLKYAEMITKYKDMKMDQDFLKKIVPNMRSNLNNIQISFKEMLPEYLFTITLEYFIHFKRRILSTCKKYLTRLNPLFQTSPTHVLQFLDDQLVYLAQSIMCTQTESKTRIIPTLLKLLSVPDVRIELYGSFLNDLDIKGSLIDIGIIEKTTGFTNINLYQNIQNILFENEYSIIQTSFIESPTATPFIKFQKVLDNQEIINFKLAFFNNGITNHISNSLLIKAYSILDPRVFALLILVKYWSKQKLINSGERYLSSFGYYNMMIHFLQSIDPPVLPCLHQHPDILKLVTSNNFENITNANIGFLNLNTMSTHQLWYLFFKFYSEFDFKNNMISITTTATATSPSANGHNHHSSRKCIKISEQFVGQPYEMVIEDPFDSNSNLGKSIYCTENMDHIMMEIYSALYRLSVQTTEAINYIFQPYPSKPLENYNYFFKKLKEFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTG4</accession>
    <name>A0A8J4PTG4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005553</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000224">
        <property type="protein sequence ID" value="KAF2073137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTG4"/>
    <dbReference type="OrthoDB" id="17625at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035144819" evidence="1">
        <location>
            <begin position="26"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="101" mass="11621" checksum="A6EF115B0310D9E5" modified="2022-05-25" version="1">MSKRFIFGCAIVFAPFLALTALTESVRRDVDKDMVDAKVSQSARLVSIQENNKQIFQNIMDSRKGDTIDYFKGKELEIENIKEIKRQIAEQKSQQQPPTKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU03</accession>
    <name>A0A8J4PU03_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pleckstrin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_005299</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000207">
        <property type="protein sequence ID" value="KAF2073386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU03"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="34"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="666"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="226"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="340"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1026"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1103"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1148"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="247"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="340"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1103"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1148"/>
            <end position="1162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2073386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1179" mass="131893" checksum="8FA7509347DDDA7F" modified="2022-05-25" version="1">MSNNQTTTTTATTASSANTADSSSNNSNFQNDDEIIYSGFLTKQGGNVHNWKIRWFVLKKGTLTYYLTNINWEFSKPRGIIYLTKRTEVKETDYRNRKFCFTVNPNYHIESQQSQSNPPTPGAPRLPTTNRIYLISAQTIFDQGKWIEKIRSALNDGSKISKKKANQIEKVKEMVQFKRKDSLPTTPTLLNRTKASETVNENIKVSLSKRKFYFYHDNLTVPLLSYVDSPEPTPIQYQNYHHRRDNNNSSNNSSSNNSSKSTSPTGSPTISPSTSPTRSSPFQFVKSKPPSVSSSSSSSSTNTPSLSTSPSSSRPASPIKNLLDEPSPFYNKPVTYIDINNNNNNNSTSSTPISNISPSSSPTNPNHLGFHRHQPKIIVHHLVRDSFQISNIGTCSFNFEILSPFDPNYAITFLPTSGIIAKGESLSVVVELIVYNHIEVDIYSTLHIRGGGEFTLYSRVETDPYLPLFLQSCSGVALSQESHKKMTNFIRSNPKMLKPLQELAHTMITEGRHIDPKSMSLPSISPRHGSGPLSRVRLSKYMLTFTRNKKNSGGDLVQSTAKIYQLLSDKFLISNSGQMDASFQFYIPNSSVAKSKYSLSLVPKNGTVACGEWFYIKCTMTVFEETEISEVIQLVINQKEIHHILISIKCENIHSGNKEIDLSKEVVLKERLGVGATGDIYKGLISIQNLKYHLSQVSFDSGGWVARRLPSSDSLTKDSVAESDMVVVAVKILHPLTEPSNEMIQDFYNEVRVLSMLNHPNVVKYVGGCTKIGQWSIVMEYVPGGNLMEVLANPVLIIPFKLVLKMAIDIAKGIHYLHSLGILHLDLKSPNLLVQSLSLGSKVNIKVADFNTCINRSRLTHFFTPSNIEDDGKKGTTLWMAPEVIRGALYSEKCDVFSFGIILWELVTRTLPYSNISFNCQIEEKVLEGMRPPRPDNVDIQYCQLMEQCWDQDPEQRPLFDSIIHSLGKILTAYEIQEQKAKASVRGLRRNHSGSSIMSFMQTIESPLTPPIITPPTIDASILDISNNNNNNNTDKDKDTKMNNKGTKSTLFRNNNIFSKQKSKRFSIEGSSIISRNDDAPSSIISTATNGSNLPFKFSPPNSFIKSSNTASTTKDNEEKKTSYLRYRPSLMGTTMLLSKSILNDLNNGNRNVDSNSSNNNDKPQKYQNSFFIPAHKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUE9</accession>
    <name>A0A8J4PUE9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ATP-dependent DNA helicase PIF1</fullName>
            <ecNumber evidence="12">5.6.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">DNA 5'-3' helicase PIF1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">DNA repair and recombination helicase PIF1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PIF1</name>
        <name evidence="15" type="ORF">CYY_005041</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the helicase family. PIF1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="12"/>
    <dbReference type="EMBL" id="AJWJ01000191">
        <property type="protein sequence ID" value="KAF2073656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUE9"/>
    <dbReference type="OrthoDB" id="19611at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18037">
        <property type="entry name" value="DEXSc_Pif1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18809">
        <property type="entry name" value="SF1_C_RecD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001986">
        <property type="entry name" value="ATP-dependent DNA helicase PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03176">
        <property type="entry name" value="PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010285">
        <property type="entry name" value="DNA_helicase_pif1-like_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049163">
        <property type="entry name" value="Pif1-like_2B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051055">
        <property type="entry name" value="PIF1_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048293">
        <property type="entry name" value="PIF1_RRM3_pfh1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642:SF5">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05970">
        <property type="entry name" value="PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21530">
        <property type="entry name" value="Pif1_2B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 12" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="9 12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="14">
        <location>
            <begin position="349"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="72"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="180"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="225"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="82"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="180"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="232"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="261"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="357"/>
            <end position="364"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2073656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="763" mass="85780" checksum="EAD20BE84F74BB62" modified="2022-05-25" version="1">MLLFKGRGLTQPLPTFYHCYSSCSPTAGIYSLHKDEIKFNFVDRKEERGYISIISCPNNLFSRLENNNITPPFHRGYNSRTNNNNNNNNNNIKRKNNNNVVFNRNFVHGNKNRSFLTNQFNKITSLTTTIASTATPTIYSRTYSTNSSSLNDYNHNDSNYEGDDDYFFDFDIDLNINNNGSTNSNSTNNSSSISDSFTTPVNNNKSSGVYVEQMIRYLVDPWPKTINKKSNKTVNKSTSNTKSSSKKIRVTSKNSEKLKSTTTTINNKPVNNNNNQQQKQQEQSLNTSPSPSTSTSVSTTNQEITFGQDENLSNQENDDQEYFDDQNVFELEHKLTKEQQKIVNLIVEGGKNVFFTGSAGTGKSFVLKHLVSKLRKKHPKSVFVTAATGIAAVNIGGTTLHSFGGIKLGVAPAQRLAVEILQSKKALQKWLDCRVLIVDEVSMIDSELFEKLDTVAQIVRENNQPFGGIQLVLVGDFFQLPPVYGNYAFESKAWKKSIDICLELTTVMRQRDLEFIDVLNNLRVGEKNDKIVNFLLDRCKRPLDVTNGVLPTKLYSTNASVDEENSAALEQLASEPHSFLAYDTGSKELLENLDRDCPAMQKLTLKVGAQVVLLRKLEKHDGLVNGSRGVVIDFVKVRSKKRFSKFYESNPMAPVVLFNDGQKVTIPPSEWGVWSEKKASRVQLPLKLAWALTIHKAQGMTLDKVECELSNSFAHGQSYVALSRVKSLEGLHLKDLRISAIKTSEKAKTFNKKFIRSIHNIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUG1</accession>
    <name>A0A8J4PUG1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">5'-nucleotidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_005836</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'(3')-deoxyribonucleotidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000243">
        <property type="protein sequence ID" value="KAF2072859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUG1"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07522">
        <property type="entry name" value="HAD_cN-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008380">
        <property type="entry name" value="HAD-SF_hydro_IG_5-nucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016695">
        <property type="entry name" value="Pur_nucleotidase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02244">
        <property type="entry name" value="HAD-IG-Ncltidse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12103">
        <property type="entry name" value="5'-NUCLEOTIDASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12103:SF12">
        <property type="entry name" value="FI20020P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05761">
        <property type="entry name" value="5_nucleotid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017434">
        <property type="entry name" value="Purine_5'-nucleotidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="424"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR017434-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR017434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2072859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="565" mass="65481" checksum="E8AEE4196E212686" modified="2022-05-25" version="1">MIKNIQRIKSNINTNFVISSQLSHCKNTISKDRFFSSTSSSSNNNNNEDRPLLNKASANLHLSSKYHSILNQITSGLSPKTESLQTTHHSVLNLSLDQLVETYNNKKTVDLSYVPTNDPQDVYINSELKLDEINVFGFDYDYTLANYSDKVQHIIYDKALNYLVDELKYPANLKELTFDPNFAIRGLHFDVNTGLLMKLDFLNNIQAGAIYHGRRPLSKEEVISIYGSMQLKRKYTYEYLKSMSDIFCLPEACLVSDIIQFFTNSNFAFEPRIIFEDITKAVGTVHLGGSLHNEIINDFPRYLNKHPLLGDFLYKLKSSGKKLFLLTNNSFHYANAGMKYLLEGQLNSKYSDWTQMFDVVMTQCDKPAFFGKGRPFRMYNYESNRYDWNEVTEFSSGKVYIGGSLQQFTKISGWRGRSVVYFGDHLYSDLVEPSQKEGWRTGVIIKELETEVEIQNSPKYRENLAELLEIEDVIRRCQFFQGDKKDSFLESLKTERYRKRLALKEPFNPNFGSLFRTHTNATMFASSLQRHADIYTSKIENLISYPLNFVFYPSRNYLPHEFKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUK8</accession>
    <name>A0A8J4PUK8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005742</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000237">
        <property type="protein sequence ID" value="KAF2072949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUK8"/>
    <dbReference type="OrthoDB" id="24428at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="572" mass="66537" checksum="D3738079EA120F79" modified="2022-05-25" version="1">MIQQTQLFFAIFRNRYLRQKIFYSFYDYCIIKNISQLDNQYDRLCLLIKHAVPVVYYIKNLDHYHVYQSHAQKHLVTHVIVSSHFYVHHYTRLLRHQQPSPVLFGLAITDRESDPLLSANEFELPATVKYYKYTLADTLTKHHIPHSVTSLFLGVLVEAGVIPSRVTTLVVNIAYSRHQEDNIKTEKMTFSVHSLPKSLTSFSYYDHMFRFNDLNLNDLPPTLMYLNVTTNRGTLTGAGYGSLKYLLYSSTKCWTIPDTITQLKIDVSRDSLDSAKLPPNLNYLEFFEQNVFNSDSNTPFLFPSSLKTLSITIGSQVPISNYNCPRGIINYKNTNYLNRNINTYIPPWAEKAHISISNETSHLPVIPPAVTHLKFISFYHRSIEFTKEIPIGYFPYNLVKLTFQYNNELEVGLIPASVTDLKLHFSMPIRENVIPTSVTKLCLVGKHMDVCPIVIPNSVTTLSFQRYIQLDQNLQIPDSVKTLKVTKINNNIFPYNLFNSTFKNVTKFCLDQGVMYEKSFDDAMPNFKNNFSNLKQIVMAFTSFKVTYHFFDQKIYKEYHYDKKRNLKFKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUZ7</accession>
    <name>A0A8J4PUZ7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_008561</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000539">
        <property type="protein sequence ID" value="KAF2070121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUZ7"/>
    <dbReference type="OrthoDB" id="9987241at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004170">
        <property type="entry name" value="WWE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037197">
        <property type="entry name" value="WWE_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02825">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117839">
        <property type="entry name" value="WWE domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50918">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="130"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="WWE" evidence="4">
        <location>
            <begin position="475"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="45"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2070121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="567" mass="63480" checksum="D68CFC027D40501F" modified="2022-05-25" version="1">MTTTDKTTEVEATAPATTTTDASTTSTTPTTTTTTTDASTTPALKRKEKEKKEEKKPAYIETRTRKKARQALDFISGSNQRQASLFNLLGKDYTGPSEQQLEMIFRLFSKNGKIGSEELAAVLRSMGKRPLTKRIDKILYECDENGSGYIEMDEFIHYMQKKAYEKAKMLGVIESDSEDDQELTKKAATKKSTTTAAAAPEKTITKQPSFFQPLKKNSSIIQFYTENAGVHTANNVEVDQYSNPVGNNFDLGLEGAFSDFTILIGMFYTFNLKCEDSLKSKGFNIIVVKTQKEFIDQLSKADVAMIIPNKNGDITTSEADFLSAVKNYHEAGKGLFLWAENEPFFAQTNWVLNSLFDSKVVGSDPGQKNLSLGKGSETQKFASHLITSGIVTLFEGRTVSYFEKVPEKLEVIATSSGNNPIIACSLATQEKNCGRVIVDTGFTKLLDEFFNAGIERYVKNACVWLLALDHRFYLGVDTQSNIPAPVEVPTWQFKHGSWFNYDADASKVVEEAYQEWLLNPYIDIRSIKSGIWSYSVNFKENTQTNIQHHAHTTREIRRVLQKVVPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PV64</accession>
    <name>A0A8J4PV64_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000096">
        <property type="protein sequence ID" value="KAF2075513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PV64"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="104" mass="10878" checksum="3B3F647690575294" modified="2022-05-25" version="1" fragment="single">KESDSSDSSDSSSSDSSDSSDSEDEKKVEKKESDSSDSSSSESEDEKEKKKESSSDSSDSSDSSSSESEDEKQVDKKKESSSDSDSSSSSSDSDSSSSDSSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVN2</accession>
    <name>A0A8J4PVN2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Hemerythrin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004205</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000144">
        <property type="protein sequence ID" value="KAF2074500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVN2"/>
    <dbReference type="OrthoDB" id="19447at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12108">
        <property type="entry name" value="Hr-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.520">
        <property type="entry name" value="nmb1532 protein domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012312">
        <property type="entry name" value="Hemerythrin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01814">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hemerythrin-like" evidence="2">
        <location>
            <begin position="235"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="178"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="448" mass="51029" checksum="BDDAECDDC68EC790" modified="2022-05-25" version="1">MEPELKNIDDSSLVVTIKLDDTEEIKQDPPVETTVEPVKVETETTPTPVEEVIVETTPVPVEEVKVETTPAPVEEVKVEPTPVPVEEVKIETTPVPVEELKVESTPAPVEEIKVEPTPVPVEEVKIETTPAPVEEVKVETTSAPIEEVKVETTPAPVQETKVETTSTPVEEVKVEAPTQVETTPVVESTSTPVQEIKTESTSEAGASSSNARPENDMKQYFEADNFHLHGWLWAHISIKRVYGLVEKKLNKGFEPKNRKEVEEFVRAVQTVADYLHSHHDHEEDFVWAWIKEFDPTTEETLTLMDKQHNEWIEKNKELDAILTRIKETKTIETSASEGFKEIFDQLAENIHYQCDVIYSHFYDEERLLLPIALKISKEGQQKVGKMIHDRIQKESNKNFSLCCMIDTAKHDKVFADSFNNTLPWFVRKVIGGLLWKKEYKWFLAILEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWN3</accession>
    <name>A0A8J4PWN3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">THH1/TOM1/TOM3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_003646</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant tobamovirus multiplication TOM1 protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000117">
        <property type="protein sequence ID" value="KAF2075036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWN3"/>
    <dbReference type="OrthoDB" id="442731at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040226">
        <property type="entry name" value="THH1/TOM1/TOM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009457">
        <property type="entry name" value="THH1/TOM1/TOM3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31142:SF33">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31142">
        <property type="entry name" value="TOBAMOVIRUS MULTIPLICATION PROTEIN 1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06454">
        <property type="entry name" value="THH1_TOM1-3_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="209"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="252"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="286"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="THH1/TOM1/TOM3" evidence="7">
        <location>
            <begin position="131"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2075036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="324" mass="37129" checksum="A207418C4D219FA7" modified="2022-05-25" version="1">MDYNSSSYLENSFCQNECGHGVCIENVCQCLPYWNGDQCDVPFKFILGKLFNAWKIGFIIIFSGLIVLLFYKIILYLLHQKLKKKQVKFLKISTYCLLVLSAISRLLYLSSDPMKQTGRVNGVFVELAGGLGLYFLFSAFLLILLYWAGLYHRVFGLARIGLFNPIAKNLFLVIDAIWLIFEIIVRVLYGLENKSFYTDGNFKLLDLIYSIYTMVIGISFSLGFMIYGTLIYHEIKKVQIKKESKENVVKKLTFIVTILSWTFLIGVVSLILIIKFNVVNHPYGALGGLTLQFIVEIALNIELLYILHKKTDKCSVKKCCDQKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX96</accession>
    <name>A0A8J4PX96_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Store-operated calcium entry-associated regulatory factor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Transmembrane protein 66</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_003255</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SARAF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000100">
        <property type="protein sequence ID" value="KAF2075426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX96"/>
    <dbReference type="OrthoDB" id="20303at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001256">
        <property type="term" value="P:regulation of store-operated calcium entry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009567">
        <property type="entry name" value="SARAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15929">
        <property type="entry name" value="STORE-OPERATED CALCIUM ENTRY-ASSOCIATED REGULATORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15929:SF0">
        <property type="entry name" value="STORE-OPERATED CALCIUM ENTRY-ASSOCIATED REGULATORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06682">
        <property type="entry name" value="SARAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0732">Signal</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035193126" description="Store-operated calcium entry-associated regulatory factor" evidence="16">
        <location>
            <begin position="26"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="256"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="262"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2075426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="287" mass="31337" checksum="041EA5771F57875F" modified="2022-05-25" version="1">MIRKSIISIFIILVSITLFSTVISAAPTRAVLLTDVKTITLSHTEMTKSRRTTPIQQLECTGGSAKKEYSLYPKTVQCYNMGSDGTDVQWKCIADLDTTVKLGKIDVSCEGYAYPEDPYILQGSCGLFYELEYTDPVKRASAAEETNYFTVFIWIAIIIFILYIIFKPSDYSTTAPYYNGQPPIPPQPYDPGYPQNGYVNPGGYYNPPPPTAPGCAPAPAANNGPGFWTGAGLGYLFGRSGRSSYYSHTPYYQTPPTFQAPRSSFSSSSRGSGVSSSSSSYGGTSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXA6</accession>
    <name>A0A8J4PXA6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 2 profile 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_004159</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000142">
        <property type="protein sequence ID" value="KAF2074532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXA6"/>
    <dbReference type="OrthoDB" id="100006at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022343">
        <property type="entry name" value="GCR1-cAMP_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022340">
        <property type="entry name" value="GPCR_GCR1_put"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112:SF44">
        <property type="entry name" value="CYCLIC AMP RECEPTOR-LIKE PROTEIN G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 157-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02001">
        <property type="entry name" value="GCR1CAMPR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02000">
        <property type="entry name" value="GCR1PLANT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="17"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="162"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="283"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="10">
        <location>
            <begin position="15"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2074532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="432" mass="49998" checksum="FF9E5C055CFC711B" modified="2022-05-25" version="1">MSSIAFIPGPDETINRIMVTVSSSLSLVGCLFIICCYLFYKELKEFQFKLIFILTINDLIISLIFLISTHLVHDQSNAYDLLPFLCSIPDSLLHYFYITSFFWNSCIANTLLQVIKYNNDKVDGYYKMYHLISWGLPSFIMAGLFLLRYFKHSDCSSRSSVYPHLLFFIPLLASWIFNIVVCFLLTKSFKEEENRLLSSSFPIPTLFNSSINNNILNNSINSSSNSNFIINNTYSNNGGHNNNCNSSSNNSNINNSISNVFNQDSVNYSLSESFSGRGKTPKIIWTSIYFLMAFGFCWVFTLISVVLKYFDTESKYILMLSYLFTPMQGFMNALIYGVNDRLRKKLKESTKRFYQNIKTQSKWYSYGNSKGSRANNEEHHPLYTYEQDDIDDADIQHYHHHPSHEYIYFRNSANYNNVLNNNNNNSSSNSNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXC7</accession>
    <name>A0A8J4PXC7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004913</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000183">
        <property type="protein sequence ID" value="KAF2073779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXC7"/>
    <dbReference type="OrthoDB" id="10432345at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="69" mass="8041" checksum="33B17A4F24917FDE" modified="2022-05-25" version="1">MSSLNDHIEWSSDFIYFNKWTSSSPGKTVYIERDNGLPMTRDFYGSVSSVFSQQIIYLAIFLLLIYISF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXF3</accession>
    <name>A0A8J4PXF3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Granulins domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_003632</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the granulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000116">
        <property type="protein sequence ID" value="KAF2075065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXF3"/>
    <dbReference type="OrthoDB" id="5854875at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.160">
        <property type="entry name" value="Granulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000118">
        <property type="entry name" value="Granulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039036">
        <property type="entry name" value="Granulin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037277">
        <property type="entry name" value="Granulin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12274">
        <property type="entry name" value="GRANULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12274:SF3">
        <property type="entry name" value="PROGRANULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00396">
        <property type="entry name" value="Granulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00277">
        <property type="entry name" value="GRAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57277">
        <property type="entry name" value="Granulin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00799">
        <property type="entry name" value="GRANULINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Granulins" evidence="6">
        <location>
            <begin position="92"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="117"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="12"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="122"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="163" mass="17915" checksum="264DD1BBA675BE5A" modified="2022-05-25" version="1">MIVSQSLHISHNNNNNNNNNNNNNNNRNHDKQHHKRPYHAIQYITPITSNGTDPNVTGTKCGDGSVCPSDNTCCLVQGGVYACCPTPQGTCCSDGQHCCPQGFSCGSTGQICIPAGNVKPYHPKEDLNTDKDTTTTSDDEEQHHHKRHHGRKHHGRKHHGGKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXJ6</accession>
    <name>A0A8J4PXJ6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutathione S-transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_002814</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000082">
        <property type="protein sequence ID" value="KAF2075878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXJ6"/>
    <dbReference type="OrthoDB" id="14146at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051:SF8">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE GSTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="8"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="50"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="209" mass="23847" checksum="F7086DA15483EF44" modified="2022-05-25" version="1">MSSATNNNIDLTLYTNRGSVSSKVSLLLNYLGLKFTTVVVNTFAGEQFTEEYKKINIHSRVPLLVDKSDKSQEPVIVYESGAILLYILKKYGTNHDLLPDSSKDLLDSDPSLVQRFESTYHRVLSALDDHLKTNKYINGDKLSAADLAIYPHFPFLQFNYLSTFSSLKNIERYYKELETIPAVNNTKTSLYEFVHSLQPFTAFIEKNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXS9</accession>
    <name>A0A8J4PXS9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003397</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000105">
        <property type="protein sequence ID" value="KAF2075320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2424457at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="22"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1011"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="29"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1699" mass="191798" checksum="D18C0897EE6F936E" modified="2022-05-25" version="1">MINFDRDFTLYSHIVEKDHEEETSEKKLNNLTLSNYTNGSTTTSDPTKENEIRLKTQAKEINSRFIQTLSTTTNYVDHAFHSVSHAADFIKESTSFYKPTSFRKNTIMILGPSGAGKSTLLNYICGVPMVVKDDDQKTAIFPLDPSSSISIVGQGNGSTTFLPTLISPTSIPGYEDCTFVDCPGDFDSNGAIFEILNEISKYKIGNGTKKLKLVLLFPQSSFTSAGAYGGGIFFDTLKKVQILVNDVLDLIDNIYMVFTHARANANTPKQTEAALNNILNSPTNGLGEFKPILEKILATKRYGFLCRPSDDNMDGDEYDPPLFSSKQELLNGIAKVEFKDIEGSNRFKLTTSNDVYDLVDGYYSKGKHIIGAIEIVSCRIVNYFKSYFTQISNSKVSLDIHRKDMISYLNQIINISDTNISFYDFIIELISKLNQNSIFPKVSDNVYSFLECLEFYWKFSKRPQQVSLNESYKSIFGLSTTLTNIFSELESIKKPNFIISQDQKTLVVQGYDLELAQVYEKVKPEMTDVIVLALNQVKINCKYQKSSQNLSIYANTWVINEQVEINLSGKDGPNQDWGAHHSGKGLPGSPGGCGGHFYGVIGESATDLSNLKIISNGGRGGNGSNGHEGETDRSTIYLDFKNHNIELDENQYSGDRNQDKNLFVNFFRDFCLIDNHTVVPRFFKRFDFLYYFHKWFLSKNDINETTIHLNKKNIKSGNKGGVMDWVDSYSTLEIIKPGRPGKPGGRGGYGGRAGKINLFNLSSDKMEINVKFEANNGANGQNGSQGRTGMATSNKIDVLLACDQKLNRSDPQECRFEKRGEIVQHCFYRPESQPALNPNTENIKDPIECQDNYIASKQEFIQFVLSTNVSLKKIDILDNLFIKRVINTCPFPMSVTEIISRLISIEKCYSELHDPTLLLFYQQLKMDLTTLVSQHKSPLSGELISTIKYIYQLICTGVFRYRSYKDTCIVVNMKPFLHMILENIKTLETLRKTDVVNMYKKSYHDNIQTKIEEAMDFVDTVQQRIQECEQQLEIDTLALINESKEEIVDIKKQNEKLETQKSQIQKHMIARMALNGLKIATTIGCTVAFGPAGLQASGAINQGVGIVGDLAIPDDPQINPGVKPVQLINPTTSNKLLASIKSNTFNEKLSALEFDISLSKYHQDIKTNPTLIYTKPDNSNLSIDERIMALNEPDNIKHLDKNKLVLLKDLEIRQAKINGRDDSFVNQLKNKRDDMVKKISNFQEHHLEMAVVVANTAASMIQDGKATKSKIDQINATIDSNNNAMIQVAKNIYEMEQFLTNQIDDIKSFLGDQKYQESSRVGLFKTQFKSKEFLREIQSFVSSFPKSDRVSICTTVFLLGECMETLVALFDKVQVYKEQQDFSNFISDVINGKDDPLNHIYTHKESIKTVIELSHRNTLVDQYNRAKIGFEQWCFPFSSYYLEGAKFNEPNTSILDKTILSAELKIAVNNLLNILDTDLSTIITSCNASPDKNIMTAKFNTSSTSAGPFFVWPHQHYGQEAKNLLAGEEISLVADTSYAQHDIIKFTKIFLSIRAQDKEENNKLQSILKHFDVCMTHSGVSFYKFNDNIYKVDNSGSSGSLTLVHQMNDPESNNQAYSKLSNNTPLLSPYTTWKFKLAPTNQENEILFQDLEKFDRVNIYLNGKGTYFNDNISSKSEKDELSVQIQSNFSDNIFSIPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXX3</accession>
    <name>A0A8J4PXX3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003776</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000123">
        <property type="protein sequence ID" value="KAF2074899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXX3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="entry name" value="30s Ribosomal Protein S19, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_uS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_uS19_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="RIBOSOMAL PROTEIN S19P FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2074899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="80" mass="9253" checksum="4B6E3B6952310FCD" modified="2022-05-25" version="1" fragment="single">MADQIKKRTFKKFSYNGVALETLLELKEDKLAELFRCRARRKLKRDTPIKHVNFLKKCRAAKAAVTQVGEKPALVKTHAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PY86</accession>
    <name>A0A8J4PY86_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003110</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000093">
        <property type="protein sequence ID" value="KAF2075605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PY86"/>
    <dbReference type="OrthoDB" id="21620at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14686">
        <property type="entry name" value="bZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051647">
        <property type="entry name" value="Mediator_comp_sub12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007:SF8">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="2">
        <location>
            <begin position="297"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="583"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="183"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="210"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="301"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="591"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="707" mass="80495" checksum="F6D65795D8F06DC7" modified="2022-05-25" version="1">MDFFLDPNNTNNDVPSDLMDVNNNNNNNNSNNSFFNFNFDNNFESNIYNIVPTSNQDVLLNINNNETSLQQQVVQQQQPIVQTQQPIVQPIVQQPQQQYDNNNYYTNNNNSNNNNNYYIPQYQADQVVNQPVYDNCNTNNNIYYQNQQQSIESNNYWTPQQQPQPQQPIQPIQQQQNIIYTYTSPSDTSTHSDSSPYQHDPSSPTTHNQTSPPSSTSGTSEQCLSSSEESNSNDLSKKVKLIKKRKLRFDPAVDIGPDLKDLLLEVSSEEFRKYKKNFYSGPDVKKSVANVINSQYRKIKNRESAQRSREKKSKYTEELEEQLDKSNKEKDDLVEENKRLSNQVEYLKNQNAKLSAFIEQNNNNNNNGNNSFFSNSNGGLQNFLNSSFSIGSPAQNSKKLLGLFLFFFLFGSFVVQPNFLQYSNNHGHNHAYTQTNTLTTGNSQMYYAKNMNPNTYISNAGPRYNGFVRTTLEKLNDNSMSSSSYLDNNNNIIKPHIMPTNNQNLVLDRGFIKNWATLPQNSKTTISFQQNNAQQLIENSNLDKQFHSVSTNTIDKNNDNKLANDACSSYSGSDEDIIELSPMPTEFKRPTTTTTTTTTTNTDSENSPSTTATSPATTNNPRLASHFYNNSTLDLLVENQLLHEPLHQNSNITKFSNSLFKFSFLFDSDQFPNGSNDISKAFLESDDHQIDITSVIVAINKRPRTQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYB4</accession>
    <name>A0A8J4PYB4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Single-stranded DNA binding protein Ssb-like OB fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002308</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000062">
        <property type="protein sequence ID" value="KAF2076403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYB4"/>
    <dbReference type="OrthoDB" id="2274046at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04491">
        <property type="entry name" value="SoSSB_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048970">
        <property type="entry name" value="OB_Ssb-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31472">
        <property type="entry name" value="OS05G0244600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31472:SF5">
        <property type="entry name" value="OS05G0244600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21473">
        <property type="entry name" value="OB_Ssb-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Single-stranded DNA binding protein Ssb-like OB fold" evidence="1">
        <location>
            <begin position="26"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="137" mass="15137" checksum="505A6567208812F3" modified="2022-05-25" version="1">MSKSPNSNNDVHQPLTKAVFAKVSDLKPMSSGHNLVLKVVSSKVVLEKNRDKKEIISEAVVGDETGTIILTVKNEQNDVVQPGNTIVLRNGIIKVFNGFMRLYVNIWGNIKLSPTPGDFTVNTENDLSAVEYELKRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYC0</accession>
    <name>A0A8J4PYC0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003038</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000090">
        <property type="protein sequence ID" value="KAF2075665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="69656at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000641">
        <property type="term" value="P:regulation of early endosome to late endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000007">
        <property type="entry name" value="DnaJ (Hsp40) homolog, subfamily C, member 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044978">
        <property type="entry name" value="GRV2/DNAJC13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045802">
        <property type="entry name" value="GRV2/DNAJC13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025640">
        <property type="entry name" value="GYF_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36983">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36983:SF2">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14237">
        <property type="entry name" value="GYF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19432">
        <property type="entry name" value="RME-8_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="1344"/>
            <end position="1419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1533"/>
            <end position="1576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1764"/>
            <end position="1860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1543"/>
            <end position="1561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1776"/>
            <end position="1786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1787"/>
            <end position="1805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1813"/>
            <end position="1826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1827"/>
            <end position="1847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1848"/>
            <end position="1860"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="2544" mass="286096" checksum="397F6BB7379E88AA" modified="2022-05-25" version="1">MANQPTSIDEFISAHHRSLSLWYTIKHSWKKKKRFLAIDPINLTTHDCGSWENTNQWAWETGIADVYPSEKHADQVVIITKKDGKKKDSLNFESDHRTDLLTDLQRSLSVVRAAEKQGKSVDQINYRHLGRAGFVYEMSVRELMTGMYKTYQAKKLKRGGRWLDVSLTVCSCSVEETDPIANVATLKENVKIRSSYFFKDIVKIEQLGDNPLGYLFYMSNGEAASDLFIVNDPKFIENVTVYAKKYVGHEIRVLKSGQTVADFGVHRIKRHGKDDDETLVSIIDFNVNKISNRHSTPVSRLLTLSTTKIVERDLTNYSIITTRPYSTIACFVRYDTDSQKLSIQYKDGLLKTYSSSARDVLIASALDFCRSVGNNNATVFTSQIVRGMKITPHHIVPNEDIQEQYIRLFENKSFDFENLSISQITLSLDGFISNIPYSGLSNVFSEKNGKVVLNCIQKLLSGVKLSGSSLSNSLGNNSPSQSDEKSELELISVVLQMLRRVVATRVGFELFTHFPHCKIPLINLLRRSIQCVDDTIAYYALDLLITLIQPAFDGDYEPGQCQANKRELFMSIDLNKTIFEIIQKHSQAGTGALVLSSSVELLTLLLCDPYSETTDSPIFNDLLSRVGHLGKDLFKLFVHPCPAISKNAGMLMNVVINEGEEEMAKQMQIVSLVEGALLRHLHDALFLKVNLNQVTPANIRNQTKKEMSKLLVNLWTAENKVCRALLERTFPRGLVQYLDSAEEAPKEALIEVKQESTGPKTIKLGVFANWRMKKILKTRELMAKAVTRVKREARPLRQFNWNMFFYQFNQDHRDANLIWNHTTREELRESIDNEIRLFQQELEAAGREVIAWNDEDFEVHYPSLKKEINVGGYYISLLVDGTSKGIKLLNPGLFFNELWQNFLLNDSPDQKALCLRAMTLVFTHYNNHPTMTHPFSNLSHLIELLESTMDVILRDRLLLFIRSLLQVKANAKLFIDNRGIYRLLELITLVHTHYTYGEEGKFLAEWYYAVEVTVEGSNTVQQQKAGPVSRTDLLELYNSKKINKSTKCWAQGTEKWKPLYQIPELRWTIMNGTTHGNVGGVLPKAELGSVILDILNALCSFRPTRHPITGAVIRPLPRVKRIICEPLNLPRIVQMLLSGHDMLVEKSATLLTALLQDNSVITSKFYLTGAFFFMMLYPNSNIIPIVKLLQIIHNKQHLITSDDSQSRRSILTPLLPEALICYLENYTPENFASKFLSDNDDPETIWNSNMRKLMREKIELHLASLPQRLHSNTTLIYEYVPIPKIVYEELEGELFCHSFYLSRLCDTRRFPNWPIKQPLELFKSVLQVWSDESIKVPQTMSVDEALEILNLKPNKGTNGKAHNYKDDEIRKAYYKLAAKYHPDKNPDGRDMFEKIQAAYELLSTINNIDNERGSDKKISLLLLTQVILYTRFMDQFRPFKYPAYTQLWKLINELIPSDKAFENSNKTPLISPATHLVYLTVEASPLNSEEITRNNGVPIIAKCFDRAVSLTFKELNISTLIIQTTPISPTFFGKQIKGDIPDPANPNNPTSPPDINNPPAPGTNTPPKTSPSAPSGALITIPTSKFNINDHSSDSADIPGEVAASLLRTIGVMAKFPGSRKQLFAHNDSLKISENICKCLYLSYSHPHIVTYALEAIYQISRQDDELQESMVKAGCIYHLMRLLFGYDHTLVNSGVQVNLESNRIMISNLHAKMAMKALRRLGGIMSEEDIEGYVEPSDKPAEAPKKPAPAVVVPTGPIAVPVADPNQLTLTGPAAPIPNATPAPENPLSSSASSIPSNAAGAPSTPTPTPQPTSYSTPTTPLSQPASSNQIALPPKINTNLNPQQVAGNNSPPQASANANANNEAYKKKLEKEFAEKVDTESNKMVRQTITAFLTPHIVIRLKIPIGSAPSKHYHKLLTELNTKHETAAFIWNNQTRDDLMDFLNKRLDDINLNPTSIKPFTTDFVYKSLSHELKVGGYYLRVYNAGPTAPTNATTTISELFVHLAEYLQKEKATHEVPVKNPDPKQYDQYLVRVASCLEAWRNTVQHGKVSPVNFNGNNRMRLLFSFLTHVDNVRIQRLVLETLLFVSSNMECINHIAVCSQETSGSLLPYMLQLYTRPNPATLDVLLKVHHNLVALPHHVMDTINYGGILYLLDIFGNNQITLENRTKSVGVMGRMIIDKVHGPKVVLLISKFIPPVFNSTMSEDPQQTVMVFDAQHENPELIWNNNTRAQLKETLATLEKQYHGLMVSNNTLNVQWKLPSEDFTITYGVGGDELIIGGVYISLFLKQPGWSLRNPRKFLLEVFDNLLRYASVELPGGAIDPKIQPLSLAIISLFQHHNVVSDQLPSTGYIEKILPLLGHSASQIRNLIVNILHSMSESQVCVDHLSKVGNLWTHVTRLLAMPNENLALTAECITKLLTLNRCEKTCLVEQAPRSDFVNTSLRLLERGKGVSPSTQAIIVNYLKQMETDALHGPTITAILDKSEIWHSYSSQSHDLFITGPAQNAVGLLTGGTSSNAVGLLTSTAFNSSTYGKSDEPPPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ89</accession>
    <name>A0A8J4PZ89_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_002970</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000088">
        <property type="protein sequence ID" value="KAF2075727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ89"/>
    <dbReference type="OrthoDB" id="17470at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499:SF136">
        <property type="entry name" value="GH08630P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499">
        <property type="entry name" value="POTASSIUM CHANNEL TETRAMERIZATION DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="53"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="TLDc" evidence="3">
        <location>
            <begin position="166"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="22"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="331" mass="37628" checksum="E9C1E97252E6FCC1" modified="2022-05-25" version="1">MSETNKSQDKLNQIKTSLADLNTLLENEHKSKNEEIKKLEKKKQELLNVTKVLDVSLLPDVINLNVGGMKYQTTKAILTKIPGTYFHVMLSGELDIKPMAHKPNTYFIDRDGTLFRYVLNYFRDGEEKLNIPNEFRNQVETEFLYYGIKIKDQIKIEEEKPQKISTIIDQSLFKTINSWIDPNNTISFDLLYQGSLNAFTGQSFHSACDGRGPTITLIQTTDGCVFGGYNSQSWNSNGKLYGDDKCFIFTLVNKHGIKPTKYLPHPSKGFVYCNSSTGPCFGYNDIDIRGQKATQSFPDAYIDSTGKGKLTLTGSEKFMIQDYEVYQCLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZA1</accession>
    <name>A0A8J4PZA1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003718</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000120">
        <property type="protein sequence ID" value="KAF2074979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZA1"/>
    <dbReference type="OrthoDB" id="29726at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47800">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47800:SF4">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="90"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="348" mass="39213" checksum="E3358827C630934A" modified="2022-05-25" version="1">MASTNNVQQPCPPMYGQTPAPQQQQPYPPMYGQQQQQQQQQYYPPMYGQQPPQPQQQYYPPMYGQQPVPHQYHTTTGPIMEPNHPRVLAKPYGSQMTYDGDSKKGKVLLKIISGHNLVSADLLSGKSDPFCKIRSYSISSLSTNVIDENLNPVWDQTFGLFIRNVDHEILVIEVIDYDSLGKNDPLGYFAINISTLPRGIEITTRENLLETKHGQVEITIKAEDFGLINVPPNYVSDYIKYRQEILVTKTKDEISKLIKAHEREVKKKLPITSNGPFVDGYPPKGYKLKSGWLKKDSKTHTNSKPYNGLGNDGKEIFSKVRVVTSNVKSNSNSVAGEVVGDVVFSFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0M2</accession>
    <name>A0A8J4Q0M2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pesticidal crystal protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000090">
        <property type="protein sequence ID" value="KAF2075669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0M2"/>
    <dbReference type="OrthoDB" id="23795at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.10">
        <property type="entry name" value="Pesticidal crystal protein, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036716">
        <property type="entry name" value="Pest_crys_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35598">
        <property type="entry name" value="ENDOTOXIN_N DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35598:SF2">
        <property type="entry name" value="PESTICIDAL CRYSTAL PROTEIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56849">
        <property type="entry name" value="delta-Endotoxin (insectocide), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2075669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="484" mass="55717" checksum="AC23C977CFE5D04F" modified="2022-05-25" version="1">MGQPDLTVVENFNPGFALGRLVLGDEYKLDNDDYLELIKGTIGCIPVVGALATSVFSIFWKKAFPTEDPYLRKEDFEKRMKKLITDMENKMDKKIDDEIVKFCQAKFESLCSAGFGFSEIEYIYDKQKRDGKPTSDAVKMYMAANHVSFRNLLLECLIFFSREDKIDLLCKLYLQTVVMYIAFLRDTHFYGIDWGLPEEYVNGVDHIKSMESKIHETIELAHGYYSKFFKKLNPPRNYTYETEMMITDESGLTYMGYPPTKFKCDYRPLLAQMTYADLTRYNLNQIWLQPTTKEGKVRETKSGNGILSPDLLGLEISMDFESYEYRLPVSNRIYIADFSNMNVMRGFFRQTEDGRNSVNSVTGTVGMSSGHSNGLFYKCFYFTEPKNLTFRVLANIDSNAQIKMVIREIPEGQIAENPDRSREVATKTHSENVAYKKLFESSSCTMAEFVSKQYQFEQPGSVYFEAEVVGGDTRDLYTLEIIGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0X0</accession>
    <name>A0A8J4Q0X0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Terpene synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_002000</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the terpene synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000052">
        <property type="protein sequence ID" value="KAF2076687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0X0"/>
    <dbReference type="OrthoDB" id="6486656at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046246">
        <property type="term" value="P:terpene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000047">
        <property type="entry name" value="Terpene synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034686">
        <property type="entry name" value="Terpene_cyclase-like_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35201">
        <property type="entry name" value="TERPENE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35201:SF3">
        <property type="entry name" value="TERPENE SYNTHASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19086">
        <property type="entry name" value="Terpene_syn_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2076687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="344" mass="40608" checksum="CF4F698206BFBA81" modified="2022-05-25" version="1">MLSLNEIKFPSSWDLTPNTDSYIEYVYQEALEMQVWRPNNKRDRIAHNNVVSLSRFFWPNADFERVVMGGELMVWFFTFDDAIDAGEFNDSECLELVRRMDSIFMEGILPENPTGPERMAHLLRLKCEKMCGKRKDTFNRFITSCIQWVDSIIPFNKIKDSGASPHLELYSFLRRVNIGAYPCVTLSECMIDHDLDISIWYDPRWIKMNENIAIVVTLINDLVSYEKEVKDQMGMLNPLYFLQNQTKSNLIESYKKMVDLIDYWVKDFLRIEESFIKSYPFKSDFQRECFHFMLDHLHYLISGSKMWSMQTPRYCSPTSPFIEMRPPSPVINPQSVENDHPQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0Y8</accession>
    <name>A0A8J4Q0Y8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DUF2421 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_001516</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000036">
        <property type="protein sequence ID" value="KAF2077195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0Y8"/>
    <dbReference type="OrthoDB" id="68611at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015743">
        <property type="term" value="P:malate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020966">
        <property type="entry name" value="ALMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052430">
        <property type="entry name" value="IVT-Associated"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47804">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47804:SF3">
        <property type="entry name" value="PROTEIN BRE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11744">
        <property type="entry name" value="ALMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="653"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="740"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="770"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="796"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="818"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="847"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="871"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="267"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="526"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="274"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="526"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2077195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1139" mass="129894" checksum="F7C339B8613FDF49" modified="2022-05-25" version="1">MAFTVVIGGAFALLPLGFDQNIMIIIPVFCIIVACNISSYSSMVAGGVVMYSTWIAGLYIFCVYHATKNYPWIAFILNAIFCFIIVSLSPGKRWFNALSVKKILLDIIIVLYFSPPNTNKNRYILENIIGAALIDASIFIGSSLFPTLASRLFIKKTIKSLHSTREFFKGVGLQLEKKMSREDTVPKNSSFAFPLYYYNPNEDNDAENVDESELLSNTTSLEEQIERDLRENQLKQTMRYQHDKDILQQSTEIVSVVPHIDLPPPTLLPVASPYQEQYTPQSEPITSSQPQPQLQPPTSISQPVTPPIDSLILPLTRSTMIEKENNDQVHTITPMKIESPQSVRFKEETIIGEEEKSSIRSSPLPATTGGYLKKEQEKVSLFGRIKRVLSVQDPPAPNESELYHLQIKIQNQMVRLTTLLAESKQERWNASLIDSFNQLLTLLVMSLKQLLSIKHSVSAGFSKMASNELLIPMAPFIECLVEEVHLQMGLMIDILKGKRGINDFDDILDISLQKLSNSIPNNNNEIIIPNNNHNNDNNNDSNNNDENNNNNSNDDEQSSGANNDNEDNNLPKIQVERSILFKSFEETDELIDKLKNFYKSLVQEYQKRDFPTLHESEISKLHFFIFGIIAFAQQQRNVAEIVIQIKKKSRTQFILFELLQHGLTLILFSFPLYFWKRLKKLAKYVSSKGGHLDNPDIMVPPSDSPIASTSLEKEFFLKSFFVALKNYLYLTFFANRKWTYPLQLTIGFTSVTVAFYYFDGITHGRFVVRGVWTCATVVLVMSPSMGATLTRGFHRIIGTIVGAFVGFLISLLCSVIPSPANAIVIIITTFIWIYTESFIQQDPRLSYAGAVAGLTFILTVYGQYYTRDFDYMYAVLRAFHIVLGVIWVIVISRLIFPFYTYKATRVKIFNTVAQMGDIFIKIVKLGLQMKDDTEDPHEHNIFDYGDQYQIQEYRANKKKSIIASLRPIRVGLDSMKVALYDVTSELILSPKKAHKYTMLVNELSNGLTRLVALETSFDCYFSENLIKAMKPINDPLNQLFMELDKSKKDIDCLLNDRVIRYRMNSASKIVDILKSFSESFQLVRSDLLKRKMLSDLHPEMIQFGSGMYCIHDFMKHHLIVLNSLSLIKHEKVLNYQVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q156</accession>
    <name>A0A8J4Q156_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">NADPH-cytochrome-P450 oxidoreductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_001753</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000044">
        <property type="protein sequence ID" value="KAF2076915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q156"/>
    <dbReference type="OrthoDB" id="1856718at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06199">
        <property type="entry name" value="SiR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000001">
        <property type="entry name" value="NADPH--cytochrome P450 reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF128">
        <property type="entry name" value="NADPH OXIDOREDUCTASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00369">
        <property type="entry name" value="FLAVODOXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="8">
        <location>
            <begin position="66"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="9">
        <location>
            <begin position="239"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2076915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="627" mass="71164" checksum="EF092B85479CEC1B" modified="2022-05-25" version="1">MKTKTLALLGAGTTLSFLLYKFFSKEAVSTEQGGAFAFNESDVTVVIKKEIKERFIQPTNVANVPILVLYGTEYGLSAEVAKKLEEEINKLTGMWARVVDMEEYEMIEFDKEQLVLIITSTYGDGVPPTTARPFFDYLDANEFDFSHIQFCVLALGDRSYPYFCQAGKMMEKYFEKCKAQKIRDRYEVDQEDWTLFDKWIETVCSNVIPTLKGLETRSDDYLYEKAKAFAGNQGKHHKKKPYQSKLLVKRLLTQQGLKDKVGYHFEFELGDSELKFIPGDALGILADNPASEVAEIISLFKKSASFKINTPGWHYQEGEQANPSQITLEHILTKCYDIHNLKPELISHLKDNCKNAGEKEKLEALLSAGTGKANTVLTKFLEEHHLVDVLKMFPSARAQNLDDTISQLAKLLPRYYSIASSMRKDDKTVALCVAVVKYNLHGTERVGIASTHMAERMKIGDKVSIYVNNNPDFRLPEDPSTPILMIGPGTGIAPFVAFIQERLSLEATGENHLFFGCRRSDEDFLYKQELENWANDGHIKLYTAFSRETSEKVYVQNRLLENSQQICDLIQAGGHIYVCGDAKYMAVDVHAALATILTKHMSIDQAEAENLLHQLEKQKRYQKDTWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1J0</accession>
    <name>A0A8J4Q1J0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Importin subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_002494</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the importin alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000069">
        <property type="protein sequence ID" value="KAF2076194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1J0"/>
    <dbReference type="OrthoDB" id="16542at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024931">
        <property type="entry name" value="Importin_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005673">
        <property type="entry name" value="Importin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2076194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="472" mass="52212" checksum="5DF6F0C1034DA953" modified="2022-05-25" version="1">MEHISIESLSGDEISNTIAQFIKLVYSDNVDKMIEGTTGLRKILSVDSPPNDQILVIGVLPRLVTFLDDSAPQILQFESCWCLSNIASGTAAHTKQVVENGALVPLVKLLSPNSKAIKDLKEQCVWAIGNITGGSPKYRDEALNFGVIDHLVDFASKTKAPSCLKTIGLTLSNLCRGKPPPPFHLVSPALSLLETLVHHPDQEINIDAVGTLGHMSTNGGPEVTQCIVDRPLLVKRMVQLMTSPIQTIMGPAIRVVGNITSSSDIEITKLVLSFGILPIIQNLFRHPKKHVRKEAFWIASNIAVGSPLFIQQMIEANIYKPIFQALIGKREDIECITEICYILLNTLGAATPQQVDYLVGSSTLDFIPSILKTMIEYPNEKICLILLETFQNILKSGDLLVKQERNREKDENPYVSLCGDDIMDLFNDIIMQADDADVQEINLIINDISDKYFGVTIINDTDNEIHEEELFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1L0</accession>
    <name>A0A8J4Q1L0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Arginine deiminase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000525</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000010">
        <property type="protein sequence ID" value="KAF2078141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1L0"/>
    <dbReference type="OrthoDB" id="5590314at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016990">
        <property type="term" value="F:arginine deiminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019546">
        <property type="term" value="P:L-arginine deiminase pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.75.10.10">
        <property type="entry name" value="L-arginine/glycine Amidinotransferase, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47271">
        <property type="entry name" value="ARGININE DEIMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47271:SF2">
        <property type="entry name" value="ARGININE DEIMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02274">
        <property type="entry name" value="ADI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19420">
        <property type="entry name" value="DDAH_eukar"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55909">
        <property type="entry name" value="Pentein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="442"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="445"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="784" mass="88700" checksum="4B3B897CFB5A55CD" modified="2022-05-25" version="1">MKKSNQLPKSSSLMSLSSIYHELNFNQVHENDTAQVVILNEPSLPQWMGSIHPNGSLYEEPLDISKAQQEHMKFRELLEKEGCTVYTVREILTGSFDQKTEDIQHRVKLEDFAFKSIIYKLDDQQDENELGEVDKELVSDQYKKKCIESMSNEQLVEVILTRPTIKLRKSERDTELLATEYSFKPLVNLVFQRDQQITTARGIVMANLSSPIRSPEVQVMKLCFQILGLPIVGEIPEPGKLEGGDFYPCGEDLCFIGVGLRSNFYAVQYMMDRDWFGTERVAVVKDYFDVNQQRMHLDTVCNLIDEKVMLILEDICGEESPMKRLVDEYVRDPLTGKYSLNRHDIEYSKYLIQEGFDLVYASDQNQKDYGCNGLNIGNGKFIVVDKATAKTIARSTKTNNQLLFVDFRTVTKMYGSVHCCSQVISRKSTESKIIDFTQASTISNSSSSDSDSSLPTSPSSTLRKQPSNGSDKIVMVAPTYFVRPRIGSELMNAHIIRQTRQAVLDDFSKLHNVLTKAGIDIHLFCHEPFHRTDQAVFVSEWFSTHDNNTLVIYPLASAAKRRERRSDILNYAFLPKYTKCLDFTPCENGDLRVIETTVQEFINNHPDQHNSNDTAPATNGEFLNFSGIVLDRKNKIAYCSIDDKVYSSKVVDLWATTLGYKVIKMKTSSPANSFLFVGEKCAIFCPEALSLTESETVLNQLYETKKKVVVITQGQMESSCAKVVELKGANSKVCLVMSDKCFNQFTKEQIDTLTQTEDTIIHCDLTNLESVGGHGLIGMIGQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1Y2</accession>
    <name>A0A8J4Q1Y2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000208</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000004">
        <property type="protein sequence ID" value="KAF2078458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1Y2"/>
    <dbReference type="OrthoDB" id="10394449at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="526" mass="60488" checksum="C05A04B009E65D26" modified="2022-05-25" version="1">MTDISFYLVWRNQYTRRLIRNLVCQGAIINVNKESLEVNHQYLSMFTNKEKQDHNISIRFMGDDIDYLEINNNNRDLINDVQLNTDADNFNLNEIHDGVRKLSLTTENQSVMATGELPPSITFLYLGGNGSYDNPPIVQPILSNLPANLQELDLLIHRDSISSPCIMPESLTDIKSVVYFDYNILKWFVVPPNKVYKSCMLRVDSMESFEWLLENKWICSVDIGRNVLEILMFRHQLPSHITKVEVYLEIVRDRSFFPPTLESLACYYGTPFSHLVNLKVLKIIGEYQTKLEKGVLPASLQELYLEYNQPLEVDVLPPNLTTLNLTSYNMPFSSNVLPPGLTTLYLHNYNQPLGTNILPSTLTDLTLHSFNQPLRAFVLPQKLKRLYMGKFTQPSIPPNSLPISLTDLTVYRFNGSFDQCQPLDNLKILRVDSLFPSLATLLANVEKLYLWIDNRDDDNPSGTCLYNTSIKSLSIQFLNAKTLYPNSFPPTLRYLSLVNAYLKPGVISRDCVLLKYREPRPYRSFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1Z0</accession>
    <name>A0A8J4Q1Z0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MRH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_001449</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000034">
        <property type="protein sequence ID" value="KAF2077260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="263283at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057709">
        <property type="entry name" value="DUF7949"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23361:SF20">
        <property type="entry name" value="MRH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23361">
        <property type="entry name" value="MUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25820">
        <property type="entry name" value="DUF7949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035193128" description="MRH domain-containing protein" evidence="4">
        <location>
            <begin position="30"/>
            <end position="2080"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="5">
        <location>
            <begin position="624"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="5">
        <location>
            <begin position="811"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="960"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1455"/>
            <end position="1522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2077260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="2080" mass="226754" checksum="8B2512B06E75445D" modified="2022-05-25" version="1">MSKYKNNIRFVLTTTVVLLLFLGLSLTDAIRVEKVKSSPILVQQQQPSSSCRYSYDIVIQSEKGLSITYPHDSVIEKLPNTNRYQVHLTFTDQQRSNGLLILEAQDETGKTFSFPLDSFQCIESTLLKHKKIEHLESSAAQNSKLSTSTNGSLTTIIVEPSNSLRGSCENSLDYNNQTVCPTLKSALALYSSVNSNVDPNILHLRLLLVDGFYDNQEKSLGQWGLDIPDNLASLLIDAYSGSNSSVVFTGVMNTIFDTPSDSPYLGQFTIRNIQFKNITPPVPSPGYSRMFRAFRLYSPKSLNGILKVNIEQCVFDTIDTGSFSLVEASVGYGSSGSSLFVSVEGSTFTNIIGGAIYASGVEFALKNTSFISNAQDAVVSYSPAFTVRNSFFSKSGGMRVHQSNSTIENSLFLNSTHNSFFSNGNSVLAITNVINSTFIGNKGSYGGALAVQSNFIQTFPATFANIINSTFINNTAIDNLNPGRGEGGAIYFYTADGLIQNSRFEGNLCNDVGGTIMFYHCKLNITSSTIVNNRQTSDFKYKGALYPYSSSLLVTDTVIEVFDGTVGGGMLCSYSDVDMIDTQVKLPFVDQDNLCYWAKKKTNCTFGSHLTDVCTPKPLPCPLENCLFKSGNTTIDLSPLSKKDGYSVSFGSGSFSIEYNFCRNTPICQTNNPMNPPSQSCQLYPHNYTFEAGTLEHTVYGIDSKGIDLRYTSNENWFGCTRSNLLRLNCDPNIDYLVTRLENYNTANGTAVCNYKITISTKYACPLAIAPNYNTCIPDQDSSSQEQNNSTEAPSNIPDYQPTCAWNYTNADGVFTSYNLSSLMLTQGSYNASFEGGNDDYTIMFNVCGYVSWCAILHPTNSQSQSCQVYPHYSFETGSLDHATVNFTSNGLVLKYPSNQANFICIRETVLNFTCDPNVDFAINSTSYKSDQTNGYRCIYQINIISKASCPIIISDSSSSGNSTSSESSYQSSEASSGNSTSSESSHQSSEDSSSGNSTSSSSSSDNSINYFTIVVVPDNFIPRYECVVSSQYNNYTVCSNITNALSYFNKTVGNMRVSAYNLNLKLLLVDGYYFDYNGLSQVAIPNINSFTVDTYSGNRSVTFFGPIAGTLFALPSYSPIFSSMILRNIMFRDITSKDKLSSVSIIKLNTDNQHLDILVDSCTFYTIDSKDHGLFNIDSSNSLLSLNTRLTIRNSEFVNINGTSAILTGLDIVLDKNDFIQNSGFRINNSSVRVQECSFIQNAGALVIVNSNTTIKTCIWQACLATPVEIVGLGYTLINKTYFIDNNGTNSGAVYVNGAGATFIRISGSVFANNTAQHPWYPTITANAVNIVSAQGQIEKSTFNETGSPTGASTIAASYSILNVTSTMISNRNNSTFAPAITFTGSLLYVSKSSLQMFKSRLALDCKQSSVELIDTIVLHPATNSSDPCYWIARDQCIFGDSLREMCYNNQSSSESSYQSSEASSSGNSTSSESSYQSSEASSSSNSTSSESSSYQSSEASSSGNSTSSESSYQSSEASSNSENSSFYYTILVSQDLLDCAYTLLPDIHMICPNITHAISYYNRLDANASPYSLNLKLLMANGTYDLPQPYNIIVPNINSFTIDTYSGKSYDVIFSGAIYNSLFVLPNSTTLTSFVVRNIVFSDIYLAGPAGSILSYESENPRQPLKILFDNCYFFNFLQLGDEYILFKISSPRSTHKHRFLFRNGGIFNITSFSSTSWHAIIDSRIASFVATDVEFDNSYFSQSDKIVVQNSIIRIDGCNFTENNGALSLQTSTLEINNSVWYSNRATGIISFDGTVEIKNSSFVDNTGSSGAIDVERGTTFVFNCVFINNFATSLNRAGALSTAMATVRVENSTFDGNTGDKGTIYSNSTTISILDITVTNRNTFHSTYRSAISLIASQADMGYSTIQVFDGTLGAGLSCINSHFNPYMTTVKLPFTNSSNSCYWVYQNDCISDNLPLLCSSAVTPPPTCKPRNGNVCGGPDQGVCTVNGCVCISPWIGTDCSSKVIPIDPPHPNTTSPNTIPTITTEPTSTPVEKDIIMDGSADSNSSPSVKSLALSQFTITTLISCFFIISSLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2I8</accession>
    <name>A0A8J4Q2I8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">IPT/TIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_001046</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000023">
        <property type="protein sequence ID" value="KAF2077659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2I8"/>
    <dbReference type="OrthoDB" id="24136at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052014">
        <property type="entry name" value="Dictyostelium_Tiger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31341">
        <property type="entry name" value="IPT/TIG DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1092"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="5">
        <location>
            <begin position="523"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="5">
        <location>
            <begin position="605"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="5">
        <location>
            <begin position="935"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1017"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1017"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1067"/>
            <end position="1081"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2077659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1134" mass="124720" checksum="78602B643FC4108B" modified="2022-05-25" version="1">MNYQYAKSYCESYTPPEGFSTGYLATITTLDEYNWVTTSQFGVLSDIWIAGSDLGNVGNWTFNVGPEKGQLMYDLYSGRSYTFTHFLLNEPSLIDGENYLYIEYENGMRNTLESTEKTFVCEFSPIDEVFIPAVHSDGGMVTVNVKGYDITTLEIKFIPLFGYPPNEFFCQDITKVGENTITCNIQSGVGQHVYIISDKDKHSIEAVWEYQPPYVSIIAPVFDSGSTITLAGDNFGNDPGRLQVTFDVIDSYIMCTNIQILTPSKKLSCTLSQSLTKVFPMEVSIDVISMTFYKTAFYDRDSNSVFSISFSQADNYQGMLDYSKKSKVDGDFGYLSVFATQDQYNLAKQFYSENLLVALGVTIDSIDPDGFKYSAGPAIDENALGFYYSFIEGTPDVSNGEIYCNFDLDSGSIHADTKQNIAIKGGLIQYGGYNPTARNYQTPFLIDSEGGTIFLELDNFGTSFSNTRVLLENKWSDYIDYFKGGISITIGAPYLGLSKYPINVAIDDDRSLLDDVNYDRTPPSIISIEPPLEASGGIITITGKSFTSHPDVITVQGIDCNAIQLTIEHRQITCSIGPGSGMKSISIIAAEKISNSFNYYYYQDPTITSIPPIPTSGGIVTIQGTGFGIDKNALSIDLCTNIEIITEDTTITCIFNQGQGDISVILTIGTYSTPPFNTRYQYPTISNIVQTNGDIDIIGDNFGWDQSKSIIKMDNDLVIGSSCTLVSNTYINCSNLATVYGPFSISNDNGVNFGTTYKYDLKPYITSTFDDECVKPQEKLTIQGGYMVSKSSTFKIKIGEQYFQVNNLDSSKSELIVPDGSGVVSLQFEIDNTRLSNPISISYCPVWPPVIKSVDYNRRTKKYEITGTGFSTAADTNTAFITSTNGVILYSLQIGEFTNSLIEVELPIDAQSGMVIVKTSELQSKKVWLDITPEIKSATSPLEQGSLITITGKYFKTIDVDGNPVKIEFSSNGNILTCQADNEVYVCPMPPGSGSVTIQVKSKSIVDDSDISEFTTTYTKESNNNNSNSKSEGSSDSDNSSNTQSSNSHNHSSNSYTNSQSKEDTENSNSNNPTNTTPTNTGKNSWRWKGPLIGISCLLAIVLSAGVIVFLRGRASSNLFNRFDRKEEGDIELM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3Q0</accession>
    <name>A0A8J4Q3Q0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_001070</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000024">
        <property type="protein sequence ID" value="KAF2077607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3Q0"/>
    <dbReference type="OrthoDB" id="4142200at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17315">
        <property type="entry name" value="MFS_GLUT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010573">
        <property type="entry name" value="MFS_Str1/Tri12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF12">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06609">
        <property type="entry name" value="TRI12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="293"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="322"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="359"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="389"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="15"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="442"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2077607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="461" mass="50192" checksum="844D6152DDF59B62" modified="2022-05-25" version="1">MVAFLNRIVDKPWFVVYLSCLGGLIFGYNTGIIVGGLGPIADKFDLGTIMQGVVVCSILLGALLGSVSSGFIADKIGRKPVIFFTAIATIGGSIGSAVATNVATICVLRLILGLGVGCASSVCPLMVAETTPQEKKGIYGAFFQISITVGIFLANIIALALKKIGESNWRWMFALGAVPGVLMFFVWIIIKESPIYLEKKNRINQSAEVQKKSSFSILFSKQNRKPMFIGFLLATLSQLTGINAFMYFSTTIFKDAGISGEYGPDIAAIILQFWNVATTLIALFAVNKVGRRVLLLTGSSVMTVCDLVIGLVFLVASGSARGWVAIVFLFIFIAGFESSIGVLFWFVINEIMDEDAKNIGAPIINALQWLFNLLLSFFFLSVVQYLGQYVMFFIFGGIGIFCTLFLKIYLPNLNAPSTTTVQEQIHNNNTVGSDEYFQHEKHSEKPLTTPELKADQPTFPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3W5</accession>
    <name>A0A8J4Q3W5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000399</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000007">
        <property type="protein sequence ID" value="KAF2078307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3W5"/>
    <dbReference type="OrthoDB" id="24334at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="475" mass="56176" checksum="6CCE3C63C6D66676" modified="2022-05-25" version="1">MNDQDIFFLIWRNRYLQQKVFLSFTDYIIIKNITQLDKKHNHLLSLNQHNIPVVYHLKDLDHYFIYFTSVYLDTTSIDIGAIPNSVKILVLNNPREDIDHDIELKEDFDIKSLPQSLTQLNFNDEYGRVYINLDEMPPNLLYLNLNECKCVINGNENFFNIPESVTQLKFGSFYPPENFNFPSNLTDLSYEYWDRSFKAPINLPPLPRNLKTLNSNCFLTCESKDNFPIGLINYTNTTERINQEKVLIIPDSVKKLVIYNQRTFSELPVIPSTVTYLELKRYYNLFNKQVKQLTNITKLIICFDRNIDYDLLIGLIPPTVTDLGLTYSKPIEKGVIPNSVTKLYLDSFKAVPIYIPNGVTTLTFLDNDQIYPEVEIPSSVRTLGAYRVNFEKFPFHLFPNVTKFWFNKSDYYLEFEEKLEMNFPNYNNNILPSNTRLVKLENLYRYRINYHFIDQKVYKEHYHLANNLLNFKQYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q521</accession>
    <name>A0A8J4Q521_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000281</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000005">
        <property type="protein sequence ID" value="KAF2078414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q521"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="108"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="182" mass="21116" checksum="7AAEF996DE6295A5" modified="2022-05-25" version="1">MNHDELPKEKKVVDQEEYRQLDDDQQEREDQEEEEEKYLYYYETSVSSSFSSSSQLVLNSFSSFDLSSSESSSLLSSLTFIPPQLQQYDDEIEINTLNFNNHHLNIGNNNSNINNNNNNNNNHSNNNNNNNNNNNSNSNNGSRTFGFNNWTVGTYPRRGTDRPRNIINIDTHSYKPNYSQMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q6L6</accession>
    <name>A0A8J4Q6L6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003606</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000115">
        <property type="protein sequence ID" value="KAF2075086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q6L6"/>
    <dbReference type="OrthoDB" id="272703at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12398">
        <property type="entry name" value="RRM_CSTF2_RNA15_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025742">
        <property type="entry name" value="CSTF2_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45735">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45735:SF2">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14327">
        <property type="entry name" value="CSTF2_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="9"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="97"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="97"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="119"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="447" mass="49386" checksum="0893375FFFB213B0" modified="2022-05-25" version="1">MANRPHSSKSVFVGNIPYEATEKEMIEIFQEVGKVVSFRLLEDKELKKSRGYGFCEYENAETALSAIRNYMEFNKRVLRVSSADNEKANEMASLMLQQQQNQSNPQQNQPVDPRGWRGNMNDQQQNQNQQNNQQMPPNMQQVSPQQMPQNMQYQSKELQSIIDGPNGPQIFKILADMKQLVQNQPDQARNLLNNNPQLAMALLHAQVSVGMISQNTANQILAMSSKQQGPGPMQGGGGGNNFGPQSPPMFNNPNISGGGGMNPNMSNPNMNMPMGGPNMNNPNMNNPNMQKGGPYGGGGMNPNMNNPNMPMGGPNMNNPNMNVNNQNMNNPNNMNNPNMPMGGPNMNMNNPIMGGGQNMPMGGPNMNNPNMNMNMNNPNMGGGQNMPLQPPPNISPSAMAAWNQLSDKQKQFTNWVVNLSPQEIEQMPMSNRAQVLHIKQVYSSQRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q7N9</accession>
    <name>A0A8J4Q7N9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">THUMP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_002660</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000075">
        <property type="protein sequence ID" value="KAF2076046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q7N9"/>
    <dbReference type="OrthoDB" id="367221at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11717">
        <property type="entry name" value="THUMP_THUMPD1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2300.10">
        <property type="entry name" value="THUMP superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040183">
        <property type="entry name" value="THUMPD1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13452">
        <property type="entry name" value="THUMP DOMAIN CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13452:SF11">
        <property type="entry name" value="THUMP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143437">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="THUMP" evidence="3">
        <location>
            <begin position="223"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="112"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="116"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2076046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="370" mass="41883" checksum="18FA22D977C2FB1E" modified="2022-05-25" version="1">MTTTTTADIVNENNKRTIEQDSGSNDKSLKSEKSQDVKTDTATSTTTKKPQSIYKGRFKGFKKHSRLFATSEKVKGLLVTCDQSREANSIGEILPLVNLYADKYFPKEVNQKVNNDEEESGDEAESDTEDNSKTTTTTTPKVEEPNKRFKRFELINSGCGGIYFVNMPIPDICPIDFMNCILIDIYKRQLLKHKQSTQVVDDILAKFPESSKKMYEQGDQSVFIPDKVLNKIRFTAKMVPITRTCRASDVDFYPIMKDVIKEKFENIKPNSFGIEMRTRNNHTLNKNDTIKDVAAMVDPEVKVDLSNPEYVFIIEVIKSSVATSILSIYRELLKFNIRDMIKIGEKTRAANTKNNTTTVDSTAATPTKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q9Z8</accession>
    <name>A0A8J4Q9Z8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Acyl-coenzyme A oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_000928</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the acyl-CoA oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000020">
        <property type="protein sequence ID" value="KAF2077741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q9Z8"/>
    <dbReference type="OrthoDB" id="538336at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003997">
        <property type="term" value="F:acyl-CoA oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033540">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA oxidase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000007">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000010">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000005">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055060">
        <property type="entry name" value="ACOX_C_alpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029320">
        <property type="entry name" value="Acyl-CoA_ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012258">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002655">
        <property type="entry name" value="Acyl-CoA_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909:SF391">
        <property type="entry name" value="ACYL-COENZYME A OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909">
        <property type="entry name" value="ELECTRON TRANSPORT OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01756">
        <property type="entry name" value="ACOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22924">
        <property type="entry name" value="ACOX_C_alpha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14749">
        <property type="entry name" value="Acyl-CoA_ox_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000168">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10 12" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-coenzyme A oxidase N-terminal" evidence="15">
        <location>
            <begin position="46"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="14">
        <location>
            <begin position="170"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase C-alpha1" evidence="16">
        <location>
            <begin position="317"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase C-terminal" evidence="13">
        <location>
            <begin position="492"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="453"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000168-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000168-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF14749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF22924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2077741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="647" mass="72844" checksum="8396A1F4DDF70386" modified="2022-05-25" version="1">MDYEQRVRSIINHLVPVDASAEIESAPVASVDNSILQGLNKVSKWDKEELRDYLEGAPQGKVIKQKIIDIVSKDPIFLPKYNMNVAEWRELTVQRAIKIIQSGVISLAEFNRDPMKYLISMETLNLVDPSLSVKLGVHFSLFGNAVLNLGTEKHHLKYIKDIDTWKLPGCFGMTELGHGSNVQGIETTATYDKTTQEFIINTPSDSAQKFWIGNAALHGQACSVFAQLYIDNVHYGVHVFVVPIRNPDGTTAENVRIMDNGHKLGLQGVDNGRLWFDNKRIPRDNLLNKFGDVEKDGTYVTSIPSKNKRFGSMVAPLVGGRVLISASALNVSKMALMIATRYAYSRKQFGSKGNETPIIEYLTHQRRLFPMIASIVALSFPINHLKQIYCEPNKTSQNVKDVHVWSSGLKAVASWTKTNILQLTRECCGGQGFSSFNQIALFKSDLEIDMTYEGDNHVLLQQVSRNLLVELQKGRYNLVSANHGSNIFDYDFQIQTLKWRESNLVSTLSSKIMAQLDQGRSLEQGWNSCLDTITLLGKAFVDRITLEYLQIGIKKASASLRSVLELCRNLYFSWRVNEDLGYFLCHDGLSHSTAQKIQDSINALCQDMLPIALDLVNSFNIPESFIHAPIAKPDYMSEFRYGLERKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UP90</accession>
    <name>A0A8J4UP90_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_009797</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000810">
        <property type="protein sequence ID" value="KAF2068881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UP90"/>
    <dbReference type="OrthoDB" id="248233at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04165">
        <property type="entry name" value="GTPBP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03694">
        <property type="entry name" value="GTPBP_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03708">
        <property type="entry name" value="GTPBP_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000014">
        <property type="entry name" value="Probable GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000091">
        <property type="entry name" value="Probable GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035531">
        <property type="entry name" value="GTPBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004160">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF9">
        <property type="entry name" value="GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03143">
        <property type="entry name" value="GTP_EFTU_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="7">
        <location>
            <begin position="198"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="634"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="642"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2068881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="660" mass="72559" checksum="34C9FADCBA8E6F0B" modified="2022-05-25" version="1">MDLFSESDPSSASDVLIMDNTQQHQQQQLNQVQQQLEETCITTTKEELTLIQDETTANISELATVSLKTTTTTTTTSTTTTTLVTNEPIMENLKPEVEFGNIEYKLKLINPTPDRLEHLTSQLKWRLGEGMGEAIYELGVEDDGTAVGLPEEDLQASIETLQAMAGKLSADLTIIRERNGSKGKVLEVLIRKYASDDFSEIRVHIVGNVDSGKSTLLGVLTRGQLDNGRGSARTNVFRHKHEIESGRTSSISSEILGFNSKGGIVNYNNLHGINPSEICEMSSKIINFVDLAGHEKYLKTTLYGMTGCYPDYCMLMIGANNGVIGMTREHLGLALALRVPVFVVVTKIDRCPENILNDTLNDIKKILKSPGSRKLPVSIKSLDDVVVAARNFISERIAPIFCVSNVTGENLNLLKSFLNLLPAKKDWENVADQPARLDIDAIYSVVGTGTVVSGTLMKGVISVGDNLLIGPDDSGIFHPTQVKSIQTKRLPVKQVRAGQTASIALKKIKRSQIRKGMVLVHPNAKPQPTREFEAEVVVLFHSTTISPHYEAVIHCCATQQCGRIIEIDKGVIRTGDKAKVKFRYLMRPEFLTVGSRFIFREGRAKGTGIITKLLPFVPEPKDIVHHKVTVAGTGSGVNGSLPKKKPSHHSRNQKKLLHRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPT5</accession>
    <name>A0A8J4UPT5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008999</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000614">
        <property type="protein sequence ID" value="KAF2069691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UPT5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="141" mass="16094" checksum="31C1259E10265A75" modified="2022-05-25" version="1">MKNKQNINSNNSNNSSNNNRHFFQFVFLPLLILITISSLFVTATTTTTSNTTPAMSDNQPRKSIDEIINSHYQDYKASQEILNDAGISSTETPPPIQFQPPAAAHHQQMHCDHDERMAKKMESALHNLKQKKEQEEKQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQD6</accession>
    <name>A0A8J4UQD6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008149</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000479">
        <property type="protein sequence ID" value="KAF2070531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQD6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="211" mass="23753" checksum="4B0810B8B8DEAC04" modified="2022-05-25" version="1">MNKSNTPNTLFEELDTLRGGDQYPSEEIKNKLINLHFPASTEDLAINLSNITSQFYALLLQSIGARYGIDEIRVQSDTLFYNLGKAKAQKALQKDPAMERDCRSFITVLISAILTSNPELKFAVEQFSPNQAIIRLTGIDRYHRAAKQYEIDSYLNFPSIVPFFQGIKDCLELKGATIKVSESSYDANSNMDCTYTFIQHSQTSASLSIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQU6</accession>
    <name>A0A8J4UQU6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Glycosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_007511</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000405">
        <property type="protein sequence ID" value="KAF2071176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="27683at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003980">
        <property type="term" value="F:UDP-glucose:glycoprotein glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06432">
        <property type="entry name" value="GT8_HUGT1_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040497">
        <property type="entry name" value="Glyco_transf_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009448">
        <property type="entry name" value="UDP-g_GGtrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040693">
        <property type="entry name" value="UGGT_TRXL_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040694">
        <property type="entry name" value="UGGT_TRXL_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040692">
        <property type="entry name" value="UGGT_TRXL_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040525">
        <property type="entry name" value="UGGT_TRXL_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11226">
        <property type="entry name" value="UDP-GLUCOSE GLYCOPROTEIN:GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11226:SF0">
        <property type="entry name" value="UDP-GLUCOSE:GLYCOPROTEIN GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18404">
        <property type="entry name" value="Glyco_transf_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18400">
        <property type="entry name" value="Thioredoxin_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18401">
        <property type="entry name" value="Thioredoxin_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18402">
        <property type="entry name" value="Thioredoxin_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18403">
        <property type="entry name" value="Thioredoxin_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06427">
        <property type="entry name" value="UDP-g_GGTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035220523" description="Glycosyltransferase" evidence="9">
        <location>
            <begin position="34"/>
            <end position="1605"/>
        </location>
    </feature>
    <feature type="domain" description="UGGT thioredoxin-like" evidence="10">
        <location>
            <begin position="55"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="UGGT thioredoxin-like" evidence="11">
        <location>
            <begin position="336"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="UGGT thioredoxin-like" evidence="12">
        <location>
            <begin position="470"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="domain" description="UDP-glucose:glycoprotein glucosyltransferase thioredoxin-like" evidence="13">
        <location>
            <begin position="755"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="domain" description="Glucosyltransferase 24 catalytic" evidence="14">
        <location>
            <begin position="1293"/>
            <end position="1560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF18402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF18403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF18404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2071176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1605" mass="181226" checksum="8F165DD014DDAD75" modified="2022-05-25" version="1">MRNTSLKSSPLSVMKMICIVFVIVCSLASFVCANEPSAIESSKVIQISMLSNWENTPLYLEAGEFLQEQDSTLFWNFIDEYIKHVNKDSNEKEQYDGIFKTLDIITSGYPEAKRALLKDMLSIRLSLRYYTAKVETMRQVVKSKLVDKDLLDTGIIQYNNKYLKDVSMVPLEMSITNLKPSEDQQTISMDSYEFDHIYPGNVNPVEMVDGVKKEVPVVVLYTNILSEHFKTSHNTLKYLARFGKIRYIIRYIVSDSTEKVHLQGYGFDLAIKNLEYKVMDDSAIKKDIKDGGESKTVVTIPDEDVKGFNFHKLQKRKPELTSKLSTFRSYLMAKSQESAELKVWEMKDLGVQTAQKISTSMDPLRSLKYINQNFPSIVQSLARTAVNESLRTIIESVQKTIPSTETTFLLNGLAVEIDSLNPIELSKILFKELECTSSIQGLGVSALSVQKMIAATADSIPLRFNLLPDKDEADIFVYLNNLETDYTYTSKWVKSIAALGASVGKPDDLFIAKNLMTTVLILDMADENSLALISELQNMIHTLVPTRFAIVLKTSRPADAPAEASDPADIAKIFLALRKSSLGNRGAMFFFSALSYFKRVYSPSGHVTINIIHSAFQAVLNQMGGNLRNLQQVLASTEYDEMFAKSNQYVSRLGLDSSLPQLFINGRVIATNTRSMGTTLPIALFDEFEQLKPLYKSGEISDTTQDYFSAILGSKHWAEIGNPLLTQFSVEIGQTGVQQVYKRLSSASNSLTKEKESSNIIKELVYFSHPESKGDQELLSVIVVGDFDQTKTQQIALQLLKKVAASPESKTRVALLPTAIDQLPSSHSIGKLLSITKKYKKQLVLSEVIRVLEAAISTGRKWSGIQSIIQDLSIKIERNDVWVSQSDNVFSLSSSIAKNYLDINVSLLEPVQLLLNGRVIEPQFEDVSAFVKESFAIQETIELLKAKSIQDILLQDSQVAKEQIPTLISNIRSLLGYYVDTGASRAKIPRSIPVSFTHAPANYENTPIRMTLLINPLSKVAQSLVPIVQKFSDKMNIAVDVILNPPTQVSDMPMKNYYSYVINLDSEFDKKTGEFISIPEGFITGLPESRVLTFAIDVPSSWLVQPIVAKYDLDNIRLKDLGEESVLEAVFELENIVIEGSCTDAYGQPPAGLELVLQPVATKSRVPQDTIVMSNVGYYQLKSNPGLWRLEIAQGRSSEIMSILDRATNKPIEYTEISVDSLYQGVRKLHVVRKPGQENKPILAPVGEVDKKKAAAAAAEAEKAKESDGFFSGLFGGGKKQDAVAKKEQKETIHIFSVASGHLYERFLKIMMLSVIKNTESPVKFWFLKNYLSPNFKEFIPKMAAEYGFEYELVTYKWPWWLRKQTEKQRIIWSYKILFLDVLFPLNIPKIIFVDADQVVRTDMKELWDMDLQGASLGYTPFCDSNTETEGFRFWKTGYWRDHLRGRPYHISALYVVDLMRFRKLSAGDHLRSTYDQLSRDANSLANLDQDLPNYLQHHVRIHSLPQEWLWCETWCDQKSKGKAKTIDLCNNPLTKTPKLENAVRIIDEWTSLDNEAKQLSEKFEPIIEKEQAEIIQQDEPKRIKNNEYIEDILGTINDAQKDLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4URY3</accession>
    <name>A0A8J4URY3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005796</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000240">
        <property type="protein sequence ID" value="KAF2072901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4URY3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035150816" evidence="1">
        <location>
            <begin position="23"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="106" mass="11351" checksum="B7B47CBA1B7C7682" modified="2022-05-25" version="1">MRAASYLLLVLVLISTFYLITIESVDCSGCVPVGKDTQGCGGDYRNLEGQIVETCMTYCVPVDYVCCGCNGINGVGKCLACPPGSTCVKSNMTTLKTQLQFWCVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UT95</accession>
    <name>A0A8J4UT95_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Alpha-1,3-glucosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_004081</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the ALG6/ALG8 glucosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="EMBL" id="AJWJ01000138">
        <property type="protein sequence ID" value="KAF2074611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UT95"/>
    <dbReference type="OrthoDB" id="1689333at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042283">
        <property type="term" value="F:dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004856">
        <property type="entry name" value="Glyco_trans_ALG6/ALG8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12413">
        <property type="entry name" value="DOLICHYL GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12413:SF2">
        <property type="entry name" value="DOLICHYL PYROPHOSPHATE GLC1MAN9GLCNAC2 ALPHA-1,3-GLUCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03155">
        <property type="entry name" value="Alg6_Alg8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="253"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="375"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="398"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="421"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="475"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="501"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2074611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="544" mass="63138" checksum="497CFE81CD0190BF" modified="2022-05-25" version="1">MSVVGELKDIFANTRYFNLIVLATFIKVLLIPSYLSTDFEVHRNWLAITSSLPISQWYYENTSEWTLDYPPFFAWFEFVLSKVAYFFDKDMLVVDNLNYKSSQTILFQRLSVIVSDFLFILATLLLSRLLYSKLPYGNSNNNNNRKTSLWYQDKQFLVSCVVILNPGLLMVDHIHFQYNGFLKGILILSIYFICRGNILIGSMLFSVLLNFKHIYMYMAPAFFIYLLKYYCFDLPRNSETQSSSLISLFNVSNFIKLGFVVLFVFVLSLGPFIVMAQIPQLLSRLFPFGRGLSHAYWAPNFWSLYNFADRLALFLGLVSKDNIKYTGNLTSGLVGSESQSHVILPQITPPITLLITIVFLIPSLYGIFKKKDNQSFLIGITQSCFTFFMFGWHVHEKAIIMITIPLGFVCLFTSTPKYSKIYFLLSTIGHYSLFPLLFKATEIPIRILVLVTYTLMIQFFIQQNNPSKFRWFEKLYLFGLIPLEIFNIIIHPIYLAHIEKFSFVSLMLTSVYCAIGIHYCYLYMYSIIFSSSPNHNTNNKKKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UTP7</accession>
    <name>A0A8J4UTP7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010591</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001222">
        <property type="protein sequence ID" value="KAF2068083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UTP7"/>
    <dbReference type="OrthoDB" id="24334at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="78"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="80"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="538" mass="62010" checksum="2DA7CA15126D3E57" modified="2022-05-25" version="1" fragment="single">TTPILFGISITDKIKDPIILANDLPQSIKYYKHTNCDKINKDFIPKSVTSVYLDTTSIDIGAIPNSVKILVLNNPREYDFDSDSDSDSDSDSDSDSDSDSDSDSDSDDDDDDDDDDDSDDSDGSDGSDGSDGSDGSDDEYYQDDKEKQKEYFNIKCLPQSLTQLNFNDICRRVYFNLDEMPPNLLYLNLNECKCVINGNGNGSIKYLIFGIFSKKLSENFFNIPESVTQLKFKSFYPLENYNLPSNLTDLSYEYCGDSYTPINLPLLPRNLKTLNLYCFLTCESKDNFPIGLINYTNNNRRINQENVLIIPNSVKKLLVYNQPTFSELPVIPSTVTHLEIARYWNLFTMQIKQLRNITKLNLCFNHNIDDELLIGLIPPTVTDLGLNYNYIKSIENGVIPNSVTKLFLCSFKTVPIYIPNSVTTLTFFDNRQIYPEIEIPSSVRTLEVYRVNFEKFPFHLFPNVTTFWFNESDYRLDFEKKLEMNFPNYNTNILPSNTRLVKFDIMNYHFIDQKVYKEHYDLANSLLTFNQGWETLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UUE7</accession>
    <name>A0A8J4UUE7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000903">
        <property type="protein sequence ID" value="KAF2068623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UUE7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="85" mass="9739" checksum="432E089F8D1DE522" modified="2022-05-25" version="1" fragment="single">FLLLHSIKASSLKLTRYIASIFRASQRTVSTQIIKLVCGLSDRILHRSILEVADLLPIDEVTSFFKNDINSHFYIKFLKTKVMPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UUQ4</accession>
    <name>A0A8J4UUQ4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Regulator of chromosome condensation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_002948</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000087">
        <property type="protein sequence ID" value="KAF2075766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UUQ4"/>
    <dbReference type="OrthoDB" id="5981550at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051210">
        <property type="entry name" value="Ub_ligase/GEF_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870:SF408">
        <property type="entry name" value="OS09G0560450 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="43"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="149"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="199"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="258"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="447"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="449"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="471" mass="51616" checksum="D8A2F896B4E89732" modified="2022-05-25" version="1">MFKKLDKLKNKVTEGINDKLGGEKEPKVTTIIQPADCTNLVGGFVIAFGDNHENQLAQTNPQSSYSNKNLVSIPSSFKPNIAYVTCGNDHCFFVAKDGKIYFTGSNKLGQFGSATSSRADQLTEYRHTVLKAKKVFCGEYNTAFISDKGQLYLTGKGELLGTLSTDTKMAPTLVPALSSYTIEDYATGPKHSYAVDKDGNIYGWGKSDNLGIGNFVKVKKITKILREPMLIESPAFVAHKVTSIACGDDHTLCLLNNGDVYAWGRGDEGQLGNQSKDLKVSPTLVESLQNYKVRQISCGAFNSSMVTVEDDCYIWGQYGEHIMTPQLLQVGYKESVKVKQIAQNSYYNIVLSTSNDIYTFGADYKIATKDSWTPTKLYINDPVLVGKKVTQVAASAANYYMIVYEGADLKYGTNIDYSKKGHKATAKDLPPMPQSTYAKNKAALFAKPSSTTTTTTTSTSSSKYDHDESVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UUR7</accession>
    <name>A0A8J4UUR7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioester reductase (TE) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_002894</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000085">
        <property type="protein sequence ID" value="KAF2075811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UUR7"/>
    <dbReference type="OrthoDB" id="20372at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44845:SF6">
        <property type="entry name" value="BETA-ALANINE-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44845">
        <property type="entry name" value="CARRIER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioester reductase (TE)" evidence="3">
        <location>
            <begin position="39"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="399" mass="46000" checksum="40E16950285FFED3" modified="2022-05-25" version="1">MSKNKESHPIEYWVNETKLPNNLSTTLKFQEKLNYTFLLTGSTGYLGAFNLKDLILNEKCQRVYCVVRSYQSFDHCKQMLKDSIHKTTAQPFGDQHLDKIYPVKGDLTKFQLGLSSLEYEMISDQVDIIISVAAQVNFSSPYKKSKITNVDCVPRLLELAVSNKKSLKRIVHVSTMAVFFDDKEIIENDNDLPLLEKLNGKSGYFQSKAIAEYRYKEASNNGFEILLIRSPGLFCNGENGYGNNSDLFQLGIKTCLIEGKTGDCKYIPNFNTAPVDWFSRVMIKMTIDKNVWIEPFNAFNPSDSTYFREYMQMVGNEFSLPELPMEEWLEFVKNSNHPTTQITLSIIKYLGYTLTKFNAPMSIKTKDYLKSINEYDGHKITRTMVFKYIYASFFFKSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UUX5</accession>
    <name>A0A8J4UUX5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WD40 repeat-containing protein SMU1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_009949</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat SMU1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000863">
        <property type="protein sequence ID" value="KAF2068728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UUX5"/>
    <dbReference type="OrthoDB" id="674604at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045184">
        <property type="entry name" value="SMU1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054080">
        <property type="entry name" value="TPR1-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21889">
        <property type="entry name" value="TPR1-like_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CTLH" evidence="10">
        <location>
            <begin position="136"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="316"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="358"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="403"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="445"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="487"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="543"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="577"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="21"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2068728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="620" mass="69417" checksum="44B54C439DE14D0F" modified="2022-05-25" version="1">METDKVKTETTPTTPTPPTPTTSTTTSTSTSTTNINNSTTINNSNNSNNNNEDEQENILTTRQIEIKNIVSSAEYSGPPKKTIYLEDNEIFDPNDPQIKEDIIRMIIQYLQDEGYTSSLLTVQDEANVKFSEHVYKISQMKKMKKAILEGDWNEVEKLCSKNTFKNHQSFLYAVYKQAYLELLEKQEYQKAFSYLTKRLKPLEGRQNNADEFKDLCYLLTCRSVQEVSSFKNWDGSKGTSREKLVEQFQSMLELENTKSTGALFRVPPHRLISLLKQSVTYQIEFGRYHPKVMPKIKTLLDDYSCFVLPNSLKFNCVGHKKNVKCVEFIGNGLTLASGSSDNTIKLWNSENGSLISTITGNGSRIWDLSSSHSGSHLASASGDGVVKIWDIQSPSKPLNPFSTKAHEGDAYTVQFHPGQNHIVTGGYDKMVHLYDARTGVLVKSFSGHNGSISKVIFNPHGNLIISGSKDSTIKFWDIVSGVCIKTLSSHLGEVTSIATNSSGSHLLSASKDNSNRLWDIRSARPIKRFKGHQNTSKNFVRSSFGPNESLIIGGSEDGYVYIWDIETCNILQKLGSPSNHMPMVYSATWSQEQSILASCSQDSSVKLWWYDPAKPVFTEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UUZ7</accession>
    <name>A0A8J4UUZ7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pesticidal crystal protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000853">
        <property type="protein sequence ID" value="KAF2068758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UUZ7"/>
    <dbReference type="OrthoDB" id="22869at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.10">
        <property type="entry name" value="Pesticidal crystal protein, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036716">
        <property type="entry name" value="Pest_crys_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38082">
        <property type="entry name" value="ENDOTOXIN_N DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38082:SF2">
        <property type="entry name" value="PESTICIDAL CRYSTAL PROTEIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56849">
        <property type="entry name" value="delta-Endotoxin (insectocide), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="537"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="590" mass="67521" checksum="439A1B2055E4331B" modified="2022-05-25" version="1">MAKRLGSKIKSTSDAFLSSTKENVKEKYNGVVDELKEDPFSIIDIEMVGEIFNDPLGFIGDLVTDKEFIGEMIGAAIECIPVVGPILSSIFSIFWPQDPKMKAVTVEELDEKLAEFKEEMVKIMDDKIKASEIETWKELCKAFFECYIFKITQMKKSVVILKKEIKDHKGVIADVPEATKERIRVTFISLLESSNNISNFCKRQKITSQVIEIYMQTIFLQFVSMIQLCSFWYQIDLDKYYILGVKKEEEGDVEVKCIKQEYHDLLVECLKNITPAVHQNLVDEKPELNKYYNNNASVMFNKNPWIYPIPLVIAPLNVPRIPTEPSLRTSIPTINVPLEQKTPFIYRIDGANMLPQPTKEWDKSFRPIPEIDPLTKCYGTFIHLSNSINISLPERKNVTIRIFGIYPKSVSNLSIGKKQFLKQDSMLPTLLRFSNLSHDEGKLLQQECVQESMFCLPPNIKNGKNFDGKIENSEQPAGFQTYLESYGGKSGFTYLKQYSHVNIISLNIGQVSTDSEINNVFNWGCIKFIELIISDLNAKDLQEIKHLESQIKEKSAKLKNLHKTDNFSEKEYKELSSEVDKLKASLNQGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UV66</accession>
    <name>A0A8J4UV66_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010331</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001044">
        <property type="protein sequence ID" value="KAF2068340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UV66"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="56" mass="6246" checksum="E4E3BF0B93678D5B" modified="2022-05-25" version="1" fragment="single">PKSRSSLSAAKIAGIVIGSVAFVAIIVVSVVYYVYKKRQYGKMKRGIEQKLQKMKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVJ1</accession>
    <name>A0A8J4UVJ1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rho GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_009961</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000866">
        <property type="protein sequence ID" value="KAF2068715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVJ1"/>
    <dbReference type="OrthoDB" id="16125at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00157">
        <property type="entry name" value="Rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001179">
        <property type="entry name" value="Rho family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="186"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="186"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="27325" checksum="413C765401312688" modified="2022-05-25" version="1">MATSGIKKTVKVVVVGDGAVGKTSLLILYTTKAFPKDYVPTVFDNFNCLETYDNKPVNLVLWDTAGQEDYDNLRPLSYPQTDVFIICYSVVKRDSLDNIKYKWLKEINLTNEGTPIILVGTKTDLREDKKTLQQLQETNQDPVSREEGIALAKEIGAVQFFECSALTGNGVNDIFANAIKAAFNKSTSSPSASSKSSSSKSSSPSTSKPSSKTTTTTSTSSSSSSSKPAEKKLSWGLFRKKDKDEKKTTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWP8</accession>
    <name>A0A8J4UWP8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000933</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000020">
        <property type="protein sequence ID" value="KAF2077746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWP8"/>
    <dbReference type="OrthoDB" id="13972at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="86" mass="9815" checksum="AF0E59A82486F423" modified="2022-05-25" version="1">MAWEIKKNPNLWIFVYVSGGYVILGSRDKSVSSSSDFCSIEDFIIKGTLQQPIIQEFGEKSFKDIYEKVARLHQLRNQKTSSPQAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWT4</accession>
    <name>A0A8J4UWT4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Histone H4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_000828</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 10">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the histone H4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000017">
        <property type="protein sequence ID" value="KAF2077866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWT4"/>
    <dbReference type="OrthoDB" id="30042at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22912">
        <property type="entry name" value="HFD_H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000012">
        <property type="entry name" value="Histone H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035425">
        <property type="entry name" value="CENP-T/H4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001951">
        <property type="entry name" value="Histone_H4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019809">
        <property type="entry name" value="Histone_H4_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10484">
        <property type="entry name" value="HISTONE H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15511">
        <property type="entry name" value="CENP-T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00623">
        <property type="entry name" value="HISTONEH4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00417">
        <property type="entry name" value="H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00047">
        <property type="entry name" value="HISTONE_H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CENP-T/Histone H4 histone fold" evidence="12">
        <location>
            <begin position="41"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF15511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2077866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="107" mass="11980" checksum="F2B55D1926C245A5" modified="2022-05-25" version="1">MSSAVGRGKSGGKVGGKVGAKRHRKVLKEYIFGITKPAIRRLARRGGVKRISLPIYEETRTVLRTFLNNVIRDSVAYTEHANRRTVTAMDVVYALKRQGRTLYGFNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXQ5</accession>
    <name>A0A8J4UXQ5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Voltage-gated hydrogen channel 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Hydrogen voltage-gated channel 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_007452</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000398">
        <property type="protein sequence ID" value="KAF2071235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXQ5"/>
    <dbReference type="OrthoDB" id="20714at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030171">
        <property type="term" value="F:voltage-gated proton channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031846">
        <property type="entry name" value="Hvcn1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46480">
        <property type="entry name" value="F20B24.22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46480:SF1">
        <property type="entry name" value="VOLTAGE-GATED HYDROGEN CHANNEL 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="135"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="93"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="268"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="8"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="274"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2071235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="331" mass="37836" checksum="D8918F852CE84CAE" modified="2022-05-25" version="1">MNNDENIDIPSPSSSSSPIPNSDAASSGGWLRSANIREHDNDSDTGSDISGYTKPKRKRPLFYPFHLNIHLPSAHDRAKWRYKLGAFIESHRVQYFIVALIILDLIITIVEMFLEETYKCKEHHEIPHAVERAEDVFKIVTLVLLGIFELEIVLLIIAFGVDFFKHPLYVLDGIIITTSIVIDVVFRDAASNLLIIFRLWRIVRVAHGVMVSVESHDKEKYKHLKHHFKQSKIRWKETEMENMELKRRLGIPLDEKIEYTIPPQLTSDSMLTNPSYSSPTFSPSTYTSMDSPISANNSRKKRHTETNSDDDDDNDGIAVRKEKGSSSSPFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZS4</accession>
    <name>A0A8J4UZS4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003893</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000129">
        <property type="protein sequence ID" value="KAF2074790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZS4"/>
    <dbReference type="OrthoDB" id="26879at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="188"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="247" mass="26990" checksum="EEB90CE29A128A99" modified="2022-05-25" version="1">MSTPTTPLKAVSPAVQKHVSSIWAKSVEVDGKAPHADNPTYPYLVTGVEQITPTREQVEAFETKRNALDRKIAPKKLETINCFYNPTSEGLVKKILQNGWKDTFQMNPEQLLLFSNANIAASHNKVMYHKMFIVRVTLGLEGSDYNITEGKQVKIKDLDSIISSFLVVYRNSLDPTPPQSPVTDRKVSSASTTPTKTTTTTTSISSPTTTKPGVTPGGLMGDGIICPSCQRQNAGNTRYCIRCGANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZZ8</accession>
    <name>A0A8J4UZZ8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Monalysin Pore-forming domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010138</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000941">
        <property type="protein sequence ID" value="KAF2068537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZZ8"/>
    <dbReference type="OrthoDB" id="2368263at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031157">
        <property type="term" value="P:regulation of aggregate size involved in sorocarp development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040927">
        <property type="entry name" value="PF_Monalysin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038768">
        <property type="entry name" value="SmlA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35884:SF1">
        <property type="entry name" value="MONALYSIN BETA BARREL PORE-FORMING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35884">
        <property type="entry name" value="SMALL AGGREGATE FORMATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18063">
        <property type="entry name" value="BB_PF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Monalysin Pore-forming" evidence="2">
        <location>
            <begin position="25"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="166" mass="18221" checksum="09E34AAAD7F93F3D" modified="2022-05-25" version="1" fragment="single">ETSEQTLSQKTGRSSWTEVSHDAGEATASSVNSSIAAGILGCNKYLDVTTGFDDNTTIAKEGNATWNQTLDPETYSVYQNCILYAFKLKPASGSMSDIMSFSMFSMSLDAAGVKVYTIGGDQFFFISFYRNDHFVVKGSDEYYEPVAFDALIAYVNSPDAQNKWNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V054</accession>
    <name>A0A8J4V054_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_003513</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000111">
        <property type="protein sequence ID" value="KAF2075165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V054"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001298">
        <property type="entry name" value="Filamin/ABP280_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF426">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00557">
        <property type="entry name" value="IG_FLMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="Filamin" evidence="6">
        <location>
            <begin position="44"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="9">
        <location>
            <begin position="589"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="904"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="926"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="7">
        <location>
            <position position="965"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2075165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1000" mass="113440" checksum="2C07434CA0755A24" modified="2022-05-25" version="1">MFYLHFNNNSNHHLKNNSCTKKNKEKNNILLNAEQVGSGLYSIVTGNGINGNIGDVGEEFSFQVLTYHSNGNQKKVGGDIVSVFIQGCDYHNLEEDVKVRVSDRMNGTFIVYYNVNKSGHYMISVMVNHVSVVGGPFKVNVSPLNYNVDILDKHTLVAGQDIVCQIQPKKTSIHPINDRIDIQLFIKEGGVLKKTVSEITTHITGVESSTYPTTTETTIQKIFKLLKTGEYIIEGTIVGVPIFQKSFTINSGPVSSKYSHLVWDGKELISENKLFILKNNTPYSEFKFLIETKDKYGNIALGSDLKDFKYYLKRSLTPIMINNNNNNNNGNSNSLDNNIDLQQQQQNQINNNLNNGLKEIQPEIHIDYESKNIKIKLNIKKEGWYCFCVKVNDQFISNSPFVIVTIKENDFQNLSNSYSLSKATFHCQIDKNNYIFDNSSNTQNILNNNNSNNKRLSNIILSISKKKIEILESYYFINIGLFEFNINPTVQIQLVGENNSNLIIKDNKSKVLLYNCPNQFLIILTSYYFMGFQSEPTFDSKIKWLKSSITAHTASKSGLSRNKMNTLKINISSRDNILEESLSILKAYDGKDLVTSRLIVKFNDEEGVDIGGISKEWYSKLSEEIGKKPIQGYYLFDQYPGSHVYHPSPLSNLVPDFKSCFKTLGKITAKSVYDSVLKADRHLSIKFAPAFYKLIVDEPLSLDDIQQVDPQLYKNKIEFITNNPMDYVNEILGEPLYFSREIYNPNHPNTGGGMSFKTVNLKPFGNLIKVTDENKQEYIDLLVQNIFYTSIKVQVDEFKEGFYSIIPQNIISIFNSKELEMLICGRDIINFDDLRLHSIFTGLFNDDIISLFWEVVNEFNELEKKLLLKFVTGSSAVPLGGFYQLCPHFTINIIPNINNNSNSSGSNSSTPLLMSTSPNPSPIPSEPNSKNNSPNLNISENTKFNKQSILCPTNTENRLPISHTCFNRLDIPFNNCFNKNILYRNLKLAITEASEGFSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0A0</accession>
    <name>A0A8J4V0A0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003299</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000102">
        <property type="protein sequence ID" value="KAF2075375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0A0"/>
    <dbReference type="OrthoDB" id="619536at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055105">
        <property type="term" value="F:ubiquitin-protein transferase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019516">
        <property type="entry name" value="Glomulin/ALF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013877">
        <property type="entry name" value="YAP-bd/ALF4/Glomulin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15430">
        <property type="entry name" value="GLOMULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15430:SF1">
        <property type="entry name" value="GLOMULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08568">
        <property type="entry name" value="Kinetochor_Ybp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="386"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="761" mass="88109" checksum="968D49F2308EF7C4" modified="2022-05-25" version="1">MEQQQQDEQQDVIRIQNEIQKDLISLIEPLLESDAAKQQLTILSESKESWVFYQGILDMIVQDTGCLHDLYNDIYWELIHCLVKSINIYSTKWDIHNPHLIDSTCTIVLKIMEWLSNNSSAREFLLVIEEFHGSSFISLSTFTKISLISLSKNALKRISVEKRFNFLKCLIPPMIRILIDDEKKQQQQEELNAQQEKQRKRNDDQEDDEDEEDDQDEEEEQDDKSIQIVGPEKEETLSQQNSDQELCWQYPLSSVLDFIHSFIMDVKTLEKSVEQQHSQELDATAIQQQQHLLLNALFKILSLEIVSLPPQPLLLNGNPLICMIGECLSLLGVSNNYILKTDQRFRAKKREMELEDLYFDEGLDEDDFEDDKDLHFDPINIKKDQEEEEEEEEEEEGEFPEEDQDNTIERQHDLDLSYTGIGYYLYLLVLKPTEQYRLPSIQSPNSILLSNLPYLIRLLQHKSYKIGFKAIQLVSFLSKRIKESSVQVDKKYLFNPVIDDPVELVLGKQLQEQNTLTTSEGQKWINEQASALLDPQYHLLQLIQCIISFLVKCPVQKIRTFTYNSFIVLLDCLCPSARFNVLCNLIGTCIFPSFTGLLVHIFKEQINKAWLSTDAEYRSYFVSPKILEVVQIPMQSGGNLMERMDAIMNGINMYRYLLIRDKETNNTGVWSKSKIMGAREQSLHPLQKNLLATQKEFKASSQGDEAAMQKVLKGVSKMGMSDQLSTQDIQKASTLVVHHIDLALDVIDRIIELQDTLTRDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0B8</accession>
    <name>A0A8J4V0B8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_009665</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000770">
        <property type="protein sequence ID" value="KAF2069017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0B8"/>
    <dbReference type="OrthoDB" id="16668at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24014">
        <property type="entry name" value="2-OXOGLUTARATE AND IRON-DEPENDENT OXYGENASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24014:SF4">
        <property type="entry name" value="2-OXOGLUTARATE AND IRON-DEPENDENT OXYGENASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25238">
        <property type="entry name" value="OGFOD2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0847">Vitamin C</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="8">
        <location>
            <begin position="337"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="72"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="88"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="130"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2069017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="434" mass="50465" checksum="77D7DF907A18BE1F" modified="2022-05-25" version="1">MSISNTINSLAATTLSNEVHLVSLGYGELRLATQYCERKTLEDLFQTEDGLIIVDSEGEEIFVDPKTNLYPLEPENTYTVISEKDLESDTEDNDLDEEEEEEEDVDDEEDEDDREDEHETTTTTTTHSHGHQEGSQLKEETCAHTHSSCCQHNDNHSSCAHDEESEQNHPCCPHDHDNEEVVLNEPQHNHTLNNQVYFKLLRMREARVEMISRLYKRLHPEIFTLSEEFFENDFIEPIRQYRKTKQEKDLLKALNKLTDTDIYQFKIFKLDFCKKLLEEIENFKNTGLPIARPNSMNNYGVVLDEIGFTQFFTDLRQNYLCLFTSILYKQYYGDCIDGHHAFVVQYKMDKDKELAFHYDESDVTVNLCLGSEFTGGSLYFKGILDQPETHSEYFEFQHQVGSALIHIGTHRHGARGLESGSRTNLILWLRRIGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V188</accession>
    <name>A0A8J4V188_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DJ-1/PfpI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_001858</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase C56 family. HSP31-like subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000047">
        <property type="protein sequence ID" value="KAF2076830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V188"/>
    <dbReference type="OrthoDB" id="543156at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019172">
        <property type="term" value="F:glyoxalase III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019243">
        <property type="term" value="P:methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03141">
        <property type="entry name" value="GATase1_Hsp31_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050325">
        <property type="entry name" value="Prot/Nucl_acid_deglycase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48094:SF11">
        <property type="entry name" value="GLUTATHIONE-INDEPENDENT GLYOXALASE HSP31-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48094">
        <property type="entry name" value="PROTEIN/NUCLEIC ACID DEGLYCASE DJ-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="DJ-1/PfpI" evidence="4">
        <location>
            <begin position="25"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2076830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="228" mass="24926" checksum="06B14D2A47A80054" modified="2022-05-25" version="1">MKVLFALTSHGELGKSNRQTGWYLSEAAHPWKALHERGIHVDFVSPRGGACPLDPTSFDLSDPVCKQFWEDPEVQRRLMNTITANQVDANSCQAIHFVGGHGVMWDFPSNIPLANLAARIYEHGGIISSVCHGAAGLLNLKVQGNYIVRGKKVACFTNAEEDAVDCTKWVPYSLENCMKERGALVQVGDKWTSNVIVDGRVITGQNPQSATELGKKLVELLNGSLPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1G9</accession>
    <name>A0A8J4V1G9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010545</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001185">
        <property type="protein sequence ID" value="KAF2068129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1G9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF173">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="773" mass="89030" checksum="D173F8FB720D2274" modified="2022-05-25" version="1" fragment="single">VWVKKEGHPYDYESYHESQIRLGIQPIITTNLFPKQLESLDNSEYKNQFDTSLLPSSLTSLTCFSPTNVPNHCNLHYLSVYIQSNQRMIFKSPDNKPITQNQIPQSFNPTNGQFTPEPQIPLDELYLSTSYICDKSVLVPKNNIIIKKLVCFRQDLPLPDSVEYYRAYIIPHNFPSSLKTIHITSFLGDSGIPPSVGFKRDPNWLHSLPPTVKKIVPLLDYSEAPMFPEIVDELLKDKYKLNHENNSIDSITSIVSNDNFRKVWRNEWLKNHIYSFIQPKLIQFVQNQDNNNNIVFNFTESFCSNHIKLRTRILCLPPNGVTYYSVISIQKKEDLFVQLPNSIKKLRIDYQGLSKVGIPSSLKHLILKSQDSELIHKEQIIIPSNIEILEFGNTCFGIQDLKKLITPNIKQIILSQKCLRGTSVNTEILTFLYERNLLKNHRYQIYEPNVPISSKTTVLIWRLDRAIPIGTIPFGVRRIIFGDNFNQTILENSIPTSVVEINFGTDFNQFLSNIYLPISLKYLSYYSTFIFPNPMVSNSLPPNCTHISIKWINYKCTGYSPYCKSLAQTPKFVKFINVGAYKFKQDYNNDSKKFKIQSFYSNEEICCQISKYIISESNKNIDIYHQETIEPKQLNTSIKSNHSLEIKLKMKRNIPLLPSLLDGSNVTSIKFGPNYNQAIVNGSLPITVSTITFGKYSSFSQQMLVLPPSITYLDIRHLTRFHEFIESFVFPPSLTELYLPDNFNESIPNGVLPNNLKILYFGECFSQKINIGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1I6</accession>
    <name>A0A8J4V1I6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_001562</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000038">
        <property type="protein sequence ID" value="KAF2077115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="27871at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032432">
        <property type="term" value="C:actin filament bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051639">
        <property type="term" value="P:actin filament network formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21219">
        <property type="entry name" value="CH_PLS_FIM_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21220">
        <property type="entry name" value="CH_PLS_FIM_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000010">
        <property type="entry name" value="Plastin-3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039959">
        <property type="entry name" value="Fimbrin/Plastin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006933">
        <property type="entry name" value="HAP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961:SF68">
        <property type="entry name" value="ACTIN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961">
        <property type="entry name" value="FIMBRIN/PLASTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01424">
        <property type="entry name" value="HAP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="1290"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="1570"/>
            <end position="1675"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="1694"/>
            <end position="1802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="35"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="96"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="151"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="319"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="439"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="671"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="981"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1058"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1154"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1224"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="104"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2077115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1809" mass="207283" checksum="0010914606304601" modified="2022-05-25" version="1">MESSTSSPRGAKELDLSLVTGITIFDDLYEQQRQQRQLELEQQQQESPDVSTANAATTTSSKVDKLDDWMHEYYLSLYGYLNQRSIRLDEFYAETENPEMSPLSREEKKTLENEHNQKESSYLRIKRNTNLKEFEPLFKYGVVPLPKQTLSSNSNRKSGIFNPVDSNNNNNSPFSTPAALPKTVVKKRPTSVPVKGLDFSSVADDVESYEEQGQQDDLPGISITSMLSNNNNNNEVSGGTVYMKKPLFSSSIGMTPPSTPDNSTCSTPAEDTTRFRRAKSISSIVKKKDGTMGIGAEFKNFTPLKTQPVKNKSYTMMFKMDAKSMLEQTEKLLQEKEEDTRIAAEIGQSLLEKNEDLEAELRNLKNKIKDQEALIEQMKSLDDEYEKLQTENRILSSQISTAQHENEQLIDKESVLSSQLNKQHLWLKSENSPLKTKTIKQQKEEIEELKFAVETLQSNQGKLIKNRDRLLSTNEQLQNSVSDLKDKLSGKIDGDEIEKLQTKIKNLRHKNKQLQIQIEQGQLLSPESRAIKHPEVLLKSAFNDLIKNEELISSTPKITNLRVLSDARSLLNRLEANIMNIDDCSLISTLVKFLDEDTKNNLPIFDIHKNIENSISNNNNNINSLVSSLDESFEHDKVNDPRSALYLLICVVSIHKYTRYDKNRIKSVDDDDEFQEERNRMKAEIQDLKNLISELQDNLHVTMNDNVQLKQDLELLNSKQQQEQQETQDKTETDQFPSFADEFKQSFGFINNDLTKELEDEIERHKQELERLKKEHEIQLSNQQLSLGMEFENAKSSLDQTISDYTLEIQQLKDKLVDLDQENCKIIKESKLLEEAIKEKDSQLADYLVQIDNLNESIRSKDSQLLDKDQEIKQYQTTISEKLSEINRKESEIQDKIQHITEKEQLIQSKEKEIERLNNELLSTLKEKENQHASFTSELSSIMTQLQQWQSLCSEKDSIIHEKEQLLSEKDAILAEKSSLLSNFEKEIHLLKDSLQQTTAKLDESNNKVSDLSNQLDQASNDKQLISMKLDESNSKVTDLANQLEESKVSFNEINGKLTDLTNQLEQTTTDKQKLLDQIDETNSKLLELGKENLILLSSIEQIKNQHEIELKSLGQTIETQIETLDQRVQDKFTHTTKSIDDLYVAKENIQAVLESKISQVNQLQSTITQLNTQIKELMTVIQDLKSQHISDKSKSESLILELQSNKEETDSLNTENLQLSTQLSEMRSLLEEKTKKHQAIKRELSMQITDMKAIVDQNKAKEAEEQSIMKQKEEANKVVRPDLEKDYFIEEEKPLVEYINRISYFSSDPDIQHVFPINYEKHISYSLYDGTLLCKLVNYAKEGTIDERVMNIVPANQIEVDQNLNLVTNSARAIGCILSPNISPQVLKTDPAEMIKVLYELVKVSVVSKININRYPNLLVLKDSNEDMKHFVAQSANQILFRWACYHLDIKKSKQTLEQLLFNPNNIIKILQILDKDVAFNPEQENGGSDENSTESKQIDWILKVSVDNFKIFPWISMESILNKNNKLIYLYISSIFQSDKGIGIDQPVSNSVETIKAVEEVLIDVEGTREERAFCMWMNSLNIKPYVNNLQQDLQDGLVILQMFDKIKPGSVSWKEVNMHPSNNYQELENCNLGINIAKSLRFSLVGIDGKDIHDGNRKLTLALIWQACKYHFLSILLNLRKSLASSNSSSAFTEADIIRWANQKVTKSGKSTCMTNFKDQTIGNGSFLIDLLESVQSGVINYNIVKSGETSEEKKLNAQYIINVARKIGCCIFVVWEDLVEVKPKMILTFISTLMCFDQERNNQNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1K9</accession>
    <name>A0A8J4V1K9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="3">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_003139</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000095">
        <property type="protein sequence ID" value="KAF2075553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1K9"/>
    <dbReference type="OrthoDB" id="433738at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050754">
        <property type="entry name" value="FKBP4/5/8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512:SF9">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0697">Rotamase</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="4">
        <location>
            <begin position="55"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2075553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="458" mass="51909" checksum="AD60973C97B844FD" modified="2022-05-25" version="1">MTSRSVVVGAYDKTLIESLQNLRVGDEIDLTTQNLVRKKLIKKGSGDNNLFPKDGDQIFIHYTGKLRNGDIFTNTRKKTQDPPHSFILGSLEEDGEMIKGWNFAVKSMIVGEIASFTFRPKVAFSDIGDGGMVPPNATCIYEIELIKFNQKVNGHGNSNSSSSSSSSNSSNDGYIIKKVIQPKSKSSFSLNSPTYESKVKISYRLLGSAEDVDPIYEQKNVMIKLGEDVNTPPILEDIIELMKPAEEYQVEIISKFYIDSFLESINRGSSGSGKKYDKLLLIVNLHSLDSSNVIKDIKQLSAQQRYDEAMERKNAGNDYFKRQHYYQAIRKYQRGAEIACELEGIEQIAEQKKHKDLAATCLVNQSVVYSSIKQWNLVVQSTTTVLEKLDNKHRKALNLRGKAYRELYEYISSIKDFKKVLNEIDANDADATRELKTSHDLYKSYLEKEKKMYSNIMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V203</accession>
    <name>A0A8J4V203_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Complex 1 LYR protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002729</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000078">
        <property type="protein sequence ID" value="KAF2075973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V203"/>
    <dbReference type="OrthoDB" id="74240at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20267">
        <property type="entry name" value="Complex1_LYR_LYRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045298">
        <property type="entry name" value="Complex1_LYR_LYRM7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47484">
        <property type="entry name" value="COMPLEX 1 PROTEIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47484:SF1">
        <property type="entry name" value="COMPLEX 1 PROTEIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="1">
        <location>
            <begin position="7"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="95" mass="11180" checksum="863B1D13FC384C90" modified="2022-05-25" version="1">MATTSRKEAIRLYRDIIRTLRPFTHTNEQGIMWKDILKESTRKEYEMAKYETDATRVVQLIMVGRDCLMRIQEGMFTGKVNQDAPPPDDNPFTRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2H1</accession>
    <name>A0A8J4V2H1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_007166</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000366">
        <property type="protein sequence ID" value="KAF2071522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2H1"/>
    <dbReference type="OrthoDB" id="21243at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.980.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053835">
        <property type="entry name" value="ASH2L-like_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21198">
        <property type="entry name" value="ASH2L-like_WH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0371">Homeobox</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="3">
        <location>
            <begin position="630"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="1">
        <location>
            <begin position="632"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="28"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="114"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="394"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="460"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="523"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="570"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="694"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="74"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="124"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="181"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="465"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="537"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="573"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="596"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="614"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="709"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="736"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="748"/>
            <end position="765"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2071522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="790" mass="89566" checksum="D2E870E138EED95A" modified="2022-05-25" version="1">MLDLKTQQQQKSNQLSSLMNQYSDDEDDAIDYYRNNNNNNNFNSFNNNNNNNNNNLKMNPKSLTTSTTSSCSPYDEDDSTYGDDESDEKKNSNGNESEDEFTVHQMLNMLSNRIESSSQRDIRSASSPNLNHFKSNHHNNNNNVSKSLHNSFDRNSSASSPIHPDDAAEAPSVSSPSSGEELSDMDDEEESDIGNNGTFSTLSINSPKNYKFGSNNTNNLLNSPQTSTGVFHQNQHVPKACTYSHCCICSYGPPPSFFTSAPTWADICYIALYCLTLSKPEVKYFHIKKDICTFIDSHYETICMRKRTSIWRQTVNMTLSHPQYFEMFQQESALENGRKGYYGLKQMHDPYEHTALNKRSRRKRRHEQKMIQEELKKNGFTNLMLNSPTTQSMTTTSFYASSSPSPSSTPVFSQSHNNTNNNNHPLISSPYMRDNEYDHSNLLQQAKKLKSISSSPLPAIPPIISPSSRPLKSSGSNSSSNFDEILNQNIDDDETTPKSLSMSAKNISYSNRNILPKLEDDDEEMFENEREVPKFHSTPSSLNSSFNNNYSNNNNNKSASIIKLIGETKPISNNNNNNNNNNNNNRPSSPMPISPTPSLSSSQNISPQWFNNGNDDDLDMDDDDDEGDESPKKRMRKTTRPEEKILLENYYQHHYGSNAKHHKEELAILSDTINWKVNRIQRWLDNRRTKDKLKTLRAATQDRSSLMYNSNQSPSLTNNNNNNNHSPPSPNPNSNLKSRQSPTHFAYNQQQTQQQQSSNQSSFSSNIKFTNNVPVNSILSNHSNINSLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2K0</accession>
    <name>A0A8J4V2K0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009844</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000827">
        <property type="protein sequence ID" value="KAF2068834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2K0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="328"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="428" mass="49018" checksum="CCB97E4029A0A074" modified="2022-05-25" version="1">MQKKNTTININKITKADSNEFKNITGHEWIRSGADGFDQAAYNKQIEIFQQFLIPVPTDEQFYIRSIFYNILIFLIAFPDAKERYNHKKDLLEEMKVYRVFGKDDSPHSSPTPGRFETYLHAIESMDIGTIVVYETQCRAIDKFNSTRWTPASIMEFESIVKVMHNVKHIRESSITKKLNGQNIHLDNIKDVIKKMYRKLSPDPGCKIDKEAKCKGQKYGSFQPYDAPTALPKPPPSESRKKEIGALVQPPPSQLSNEDRIIILLEDTNKNLNNLVTRVRELESKLGYKSDPFIIDGDNEHDNFGLPPTSLEDSYHLDDNLQRTKKTITTTTTTSSSSLKNKKLQQYGSDDIDPNKHSRKRKNQTTPPTSEDENEPHEKDASHNLIKGTVVKRVNVVTSKTQPSEEDHKSKKKRMVISNPQVNKCILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2Q6</accession>
    <name>A0A8J4V2Q6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_002483</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000068">
        <property type="protein sequence ID" value="KAF2076243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2Q6"/>
    <dbReference type="OrthoDB" id="275748at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12241">
        <property type="entry name" value="RRM_SF3B14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000604">
        <property type="entry name" value="Splicing factor 3B, subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034150">
        <property type="entry name" value="SF3B6_RRM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="21"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2076243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="126" mass="14423" checksum="EC4C1A06ADDBCD7A" modified="2022-05-25" version="1">MASLANVAALKKSKLPPEINRILYVKNLPFKITSAELYEIFGAYGAIRQIRLGNSTETMGTAFVVYEDIFDARNACEHLSGYNVNNRYLVVLYHQQQKQAKKMDQSKRRQELDILKQKVGDIISNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V397</accession>
    <name>A0A8J4V397_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">medium-chain acyl-CoA ligase</fullName>
            <ecNumber evidence="5">6.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_006295</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a medium-chain fatty acid + ATP + CoA = a medium-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:48340"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:59558"/>
            <dbReference type="ChEBI" id="CHEBI:90546"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:48341"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.2" evidence="5"/>
    <dbReference type="EMBL" id="AJWJ01000285">
        <property type="protein sequence ID" value="KAF2072377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V397"/>
    <dbReference type="OrthoDB" id="6614653at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004321">
        <property type="term" value="F:fatty-acyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031956">
        <property type="term" value="F:medium-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05971">
        <property type="entry name" value="MACS_like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000063">
        <property type="entry name" value="Acetyl-coenzyme A synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000005">
        <property type="entry name" value="Acyl-coenzyme A synthetase ACSM5, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049515">
        <property type="entry name" value="MACS_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051087">
        <property type="entry name" value="Mitochondrial_ACSM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43605:SF10">
        <property type="entry name" value="ACYL-COA SYNTHETASE MEDIUM CHAIN FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43605">
        <property type="entry name" value="ACYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="8">
        <location>
            <begin position="118"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="9">
        <location>
            <begin position="564"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="241"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2072377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="657" mass="73801" checksum="884D3A5249701726" modified="2022-05-25" version="1">MNGLINTRSINQLIKVYPQFQSYHIRPLSSLNSFQTKSLKSRITSPIGNNNITLNTIIPTLTTTTTTTTTKRLYSTFLNETNLIKGDNYQDTYNNFKWTIPEYFNIGVEVCDKHVKGGNKDKVAIKYIQEDGKTIKSYTFNDLYNESNQLCNAFKDFNIQQGDRVGVLLSQGFETALSHTSVIRSGMISIPLFTLFGPDALEYRLNNSEASCVLTDLENLPKLISISNNVPSLKKIIVFNNNNNNNNNNNNNNNNNNNNNNNNNNNNNSNNNLIINWEDIKNQYKNIKFEPIKTKSEDPAVIIFTSGTTGNPKGCLHAQRVLLGHLPGVQFPQNFFPQKSNDYVFYTPADWAWIGGLFDVLLPSLYYGVTVLAHRALKFDPVKIGEIMLENKVTTCFLPPTALKMMRLNSHVPKNTNMLSIGSGGESLGDRLLEWGKETFGGITINEFYGQTEANLIVGNCSSIMPVKAGSMGKAIPGHKVEIIDKHGNILPPNTVGDIALLTPDPVVFLKYWNNEKATSSKFVDKKWLVTGDLGKKDSDGYLWYVSRDDDIINSSGYRIGPSEIEHCLLRLPYVLMSAAVGVPDPIRGEIVKAYIVLKENCKPSKELELEIQNFVKTNLAAHEYPRQIEFIDQLPMTTTGKIIRKDLRLRNSNNKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3H1</accession>
    <name>A0A8J4V3H1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_001890</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000048">
        <property type="protein sequence ID" value="KAF2076813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3H1"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF288">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13606">
        <property type="entry name" value="Ank_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="361"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="394"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="483"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="510"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2076813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="801" mass="90610" checksum="B40033A27119B7AD" modified="2022-05-25" version="1">MSRIIDGLSLIYINGYVDNNDIRQLTLVCKDFYYYLCGNNHRGIIKNLRTSSNSSSEGDRSVFNQYIESHYLALYIKLLSVFDLNAFEKISFSSTSTATITPTTTNSIHALQIKQKYILLKEKVDLLYKCKINFDQYLYNNEAYSRFMSFEISNNSNNTNNSSNKKRFTLDSLLCFMVIYQQDNLLHLIINVLSTLIRTIHCNIFPPPPRPSLSSSSSSPHHLKMSNNNNPLFSQYQSQIPNNQDYEYLLSRVKILNDSINLSVFPLYLAVIKGNANLIKFLLSSMPNLYSIQCKLPVSHKSTLFYAPNREIALLLLQLGCRVNEVDSFGMLPIHFHSLNGNLDVVKCLIDDSSINSQDSYQNTPLHLSSLKGQFLVIKFLMSSGARFNVTNKQGRLPIHNACINGNVEIVKFFLELYLKQSLRATGLKTSGSGDGSNLISTQITDKENNTPLDLSVLNNHFAVSYEILKYEGCIINKDELNFKDARRIGSGAFADVYLLYWRNKNVAVKRVKFNKILESGKTETWIREKFILEVGLMVKLSHLPSFVKLYGSCVETDELMLILEFCGNGSLYNLLNTVNNEQVISTLPSINTLSMSIVSGMAYLHCLSPQIIHRDLTSQNVLIDSNGCAKIADFGISRFKNDYGDKTMTSIGNPRWRAPEVTKGQKYSEKVDIFGFGMILYEMFTRKVPFYQFEPVQASFKIANGERPELDSNVIDYRWVSLIQQCWDQNPQNRPSFDSIQSTIQQLPIANIPKIHSLGSAGNSANNSMDDFSDSTQSMDYTVGTSASTYSYNTGGKMNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3Y4</accession>
    <name>A0A8J4V3Y4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_005653</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000231">
        <property type="protein sequence ID" value="KAF2073037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3Y4"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08217">
        <property type="entry name" value="STKc_Nek2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000097">
        <property type="entry name" value="Probable serine/threonine-protein kinase nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF10">
        <property type="entry name" value="NIMA-RELATED KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="4"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2073037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="377" mass="44356" checksum="D68EDE790DD68ACD" modified="2022-05-25" version="1">MDAYEVLGPLGKGSFGSVSKIRRKEDGRVLVWKEICYGHMQEREKQLLVNEVNILQKLKHSNIVRYYDRIIDSASYKLYIIMEFCSGGDLSQLIKKCRNDRVYIEEEVIWRTLIQILSALQEIHNRKDGVILHRDIKPGNLFLDENRNIKLGDFGLAKILNINSLYAHTFVGTPYYMSPEQIQEYPYNEKSDVWSVGCLIYEMACLSPPFEATTQAILKSKIQAGRYNPLPPQYSENLTKIISMMINTDQSRRPNVDELLNTNFLAIRVKERKVQQYYSNLKQMEVDLKNREKLLIERERAVLVREQQIAQKEKEIMEKENYSQNRMNIQQQQQQQPQMMVKPVYSAEEERMRSSSENVPFQPYQMKRAYTTPLNFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4S3</accession>
    <name>A0A8J4V4S3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007606</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000416">
        <property type="protein sequence ID" value="KAF2071069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4S3"/>
    <dbReference type="OrthoDB" id="10572015at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035325241" evidence="2">
        <location>
            <begin position="22"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="210" mass="23394" checksum="645128B7532BF985" modified="2022-05-25" version="1">MVKILNILLATIVFFAAVAISVDLPVYTNSGYSENIINDSLDAHIEESTDCREGMEKCIKATTLTDNGFYGFKLNDTTGFNSADSKYIEANMFVEYNGDRGQTVIWTSVQTVSKPARAYLNETWFVSGNLTEGWQFVRVKLSTLNVEAATFNSFRFGASLKNTTIYIGEIHFTDAPTSLEPLGYVDNSSSSLMINSLFVFVLPFLFLLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4V4</accession>
    <name>A0A8J4V4V4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004739</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000173">
        <property type="protein sequence ID" value="KAF2073952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4V4"/>
    <dbReference type="OrthoDB" id="18378at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd11709">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050672">
        <property type="entry name" value="FBXO45-Fsn/SPSB_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12245">
        <property type="entry name" value="SPRY DOMAIN CONTAINING SOCS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12245:SF5">
        <property type="entry name" value="SPRY DOMAIN-CONTAINING SOCS BOX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="62"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="2">
        <location>
            <begin position="116"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="34890" checksum="405FAF3DD662606D" modified="2022-05-25" version="1">MELCYGETSYSFIASQEPQDEANQFEMEYYKSLPHHHHMVVPPSNAIVDINDLFQQQQSGNYRELQSLPFEILLHIMQYLEVESLVQVSRTNHLFNYICNDDELWKYICINKFGWTIKKSKSLYSDWKTFYRSFNIYKGSWDSNKMGKMILSNNKKSITHAGDYLGSYQSARGTLPIKSGLTYWEFQVNCLNANQTGFHLVIGLVPESFSIWQTYLTSNGGWGYLADGRKAYNSGNGVSYAQGFAQGSVVGVLVDMNLKTMTFYHNGKCQGVAFTNVEGTVYPGVSLLTGGQSISFIDNPQFPKLDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6I5</accession>
    <name>A0A8J4V6I5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">rRNA methyltransferase 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_005808</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000241">
        <property type="protein sequence ID" value="KAF2072884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6I5"/>
    <dbReference type="OrthoDB" id="270651at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016435">
        <property type="term" value="F:rRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18095">
        <property type="entry name" value="SpoU-like_rRNA-MTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047182">
        <property type="entry name" value="MRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013123">
        <property type="entry name" value="SpoU_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46103">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46103:SF2">
        <property type="entry name" value="TRNA_RRNA METHYLTRANSFERASE SPOU TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08032">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00967">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="RNA 2-O ribose methyltransferase substrate binding" evidence="8">
        <location>
            <begin position="130"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="49"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="228"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="470"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="64"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="228"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="470"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="508"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2072884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="518" mass="59530" checksum="3FC94C7871B9678D" modified="2022-05-25" version="1">MNHIQKGYLSLHLGFQRCLNNTTTSGITGIHITKSYYSTGNRERFSFANKDKRSSSHYQQRDYNSSNKSNNYNNNNTSRYEQRDRKPSSRFEQKVALENNNNKNRYSVKAASISDNAEFPKDNNPNDEVNISGFRSVLAIFEHRPQDLRRLYLSNEFYQENKVELKPLVKHFSDNKIPYRIFYNEEVSQLEKIAGTVHHEGIAIAVKERQRVDAKAVVMKELEKSKQFHTPSPTHSFTSIAKSQNHKTKLNSHTTTGSGARPIVILLDNVENPQNIGAIVRSCVSFGVESVFFYNEYDMHKTGNLINPFSPQSYRASRGALEYADVVQLSHREDVFDMIKTFKDNRWEIVSTSSHQYSKSVNLYSDESNKLLGLGPLLLIFGSESKGVSKHWIKFSTRTLSVPGTGMVDCLNVSQAISVILGEAWRIQGKQIEPFDSEQIKQAKAIQRPTEITPKLLGINKEDIYKDEEEDLVDVETQQEDQEDQEIEYQDARQNKKKNFKKSSSTTNRKRVTKYHRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7D8</accession>
    <name>A0A8J4V7D8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_001931</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000050">
        <property type="protein sequence ID" value="KAF2076742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7D8"/>
    <dbReference type="OrthoDB" id="10259130at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000039">
        <property type="entry name" value="Cysteine proteinase 2, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0458">Lysosome</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035253813" evidence="9">
        <location>
            <begin position="20"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="11">
        <location>
            <begin position="35"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="10">
        <location>
            <begin position="119"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2076742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="339" mass="38003" checksum="E7906886116073EA" modified="2022-05-25" version="1">MLKKLTVLALMILVAVVAGKHHPRRFHENHYKSQFIQWMNERNILYSSRDFHKRYDNFKNNVDYIANWNEAGSSTILGLTDFADLSNREYRQTYLGTTFDASDLLEQDSGLNQIFVGDLPNHLDWRVKGAVSHVKDQGRCGSCWSFSTVGALEGSNFLSTGKMETLSEQQLVDCTREYGNYGCNGGLMEPSFQYIIDNGGIMSEEAYPYTATDKAQCKFDKSKVVATMSAFNATVRGSEESLLQAVQHTPIAVAIDAGQRSFQLYKAGVYFDQGCSSYRLSHAVLLVGYGEDTTTTEHSQYWIVKNSWGTKWGNQGYIFMAKDASNHCGIASMSSYAII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8W3</accession>
    <name>A0A8J4V8W3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_003245</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000099">
        <property type="protein sequence ID" value="KAF2075464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8W3"/>
    <dbReference type="OrthoDB" id="411785at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000217">
        <property type="entry name" value="Methyltransferase protein 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051419">
        <property type="entry name" value="Lys/N-term_MeTrsfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12176:SF79">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12176">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="4">
        <location>
            <begin position="48"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2075464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="199" mass="23105" checksum="CD239D1649892FAF" modified="2022-05-25" version="1">MSEEETYGSCSYWNTRYQQESTPFDWYQSFIGLKSYFESLIKLQDDILMVGCGNSLLSEQMYEAGYKSIVSMDISPVVIEQQNLRYRDKYPDLQFLVENALDMSFQDNTFDIVLDKGTFDTIMCGDNSQENALIMCQEINRVLKPGGKYIIISYGAPKDRESHFSNACEWKRIDIQTIKKPDSKKSTSVHYIYIMTKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJ44</accession>
    <name>A0A8J4PJ44_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010578</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001211">
        <property type="protein sequence ID" value="KAF2068095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJ44"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036811">
        <property type="entry name" value="Ubol_cytC_Rdtase_hinge_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81531">
        <property type="entry name" value="Non-heme 11 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="105" mass="11991" checksum="BEBB4E527AC63BCF" modified="2022-05-25" version="1">MDSRQLTNGEVADVFSSIDPSCEEQIRKRALCQASVNSESEYAKNCAKQHLDMFLCLAKCAQSAILKECLVANNYDLYKCPKQVDEFHKNCNNEDSLPPYYKQMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJI8</accession>
    <name>A0A8J4PJI8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001148">
        <property type="protein sequence ID" value="KAF2068184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJI8"/>
    <dbReference type="OrthoDB" id="15675at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="194" mass="22720" checksum="FF20AFE733CD9F8E" modified="2022-05-25" version="1">MPEADFINEKKWDDRFEKAKLAYKQNKFVRWFKSKRPFPWIYVFSFIVNIPMLVALVILTGVRWTSDCSFVGGFLIVYIIIFIINLLYTIYFHKTMKLKKPRTCISVMGDCGFSSFFLFSAISVVIFIICEFLRRGTEYTQCYKSNSDIYHLLRGAAGIDIFFLCGFYIINFFNVCCSCYKVGNSNTSKKRLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJY1</accession>
    <name>A0A8J4PJY1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutathione S-transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_010355</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001059">
        <property type="protein sequence ID" value="KAF2068318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJY1"/>
    <dbReference type="OrthoDB" id="14146at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03048">
        <property type="entry name" value="GST_N_Ure2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051:SF8">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE GSTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01151">
        <property type="entry name" value="Main.2:_Nu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="4">
        <location>
            <begin position="91"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="225" mass="25932" checksum="C48510F92F282DBB" modified="2022-05-25" version="1">MTTIDFYGASTSNNYKVHLLFEELGITYNYKPVNISTGEQFDKEFLKISPNNKVPAVVDHSFSPALTLFESAAILTFYADKENKLLPNQSRLRERADVLQWLNWAGSGLSPFFGQFTHFTYFAPEKVPYAQTRYINELVRLLNVLDKQLQDKDYVAANEFSIADICIWGWAVYLPYLGEPLGWNNQDQFVNIKKWLALIDSRPTAKKVLVKVEEVLKSKTFKKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PK13</accession>
    <name>A0A8J4PK13_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010584</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001216">
        <property type="protein sequence ID" value="KAF2068090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PK13"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005547">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000286">
        <property type="entry name" value="PH-protein kinase domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12752:SF9">
        <property type="entry name" value="KRAMER, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12752">
        <property type="entry name" value="PHOSPHOINOSITOL 3-PHOSPHATE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="8"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="452" mass="50489" checksum="BE07566E0A974B37" modified="2022-05-25" version="1" fragment="single">VSVSQLAPSDREGWLTKQGGSIRTWRRRWFVLKGKKLFYFKNKSDIEATGLIELEANSIVKEEKDRDKKKKYMFTVATFKRTFFIYAETEQDMKQWMDAIKRNIDGDGIKSTGEVTSPKISSTGGETSTSPKSQGVSVEKQLSASSPRSKISNAKSVIPFLREEDSKVLEFWQIWLESIPPQSDLSSGTSIEFHVATSIDMQKLTWRTAGPQNIFIQKMVDFFWNVGAPESEIDRLNDVGAIINPVKIGSWIDMSDKGGMDGGWYFPVDIPLKLAIEASDEGEPTRKLAEWAEANEVSNCYSVGRDMGAAPPRQTEIRLKLPGPDFIAQLNLAVDAFKTFEFPQIPPNALDILRQNSSGLESAGLCLSVITSSEGFVRLGLLIPKPSREVVSQLCEFGQANKERISKFETALGGAGPAFVEFQYLQKGFGYTVYKEGFDIVFHYFVGEDQSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PK57</accession>
    <name>A0A8J4PK57_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_010422</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001102">
        <property type="protein sequence ID" value="KAF2068253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PK57"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF29">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035294467" description="EGF-like domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="3">
        <location>
            <begin position="425"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="2">
        <location>
            <begin position="656"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="742"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2068253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="742" mass="83733" checksum="F019D0B9339266B8" modified="2022-05-25" version="1" fragment="single">MNSFFIFLSVFLFFTVAHGLQGSYLPDPTYNFNQYALYDGNCSLYYSVFLKDATGTIEYLGNPSIRKEIAVYNQNGTDASFQLRILVPIGTGIYNFVVSMLESNSVPMDIPYSCQEFPTPVIKDLGFTNPIQETSPYKYSFLVENKEKYFNTLRTALNTTEWNYSGRFKDLKTYTINLTPIVIPSINSYIGITITFPNGFIRVFDIVIPYKVLHQDIRTYLYLPSTEILTNKYALTLNYQRLSDLKVRVSSTSGETTNSFNLPVYGNPLNATCKGVFLLGAFENNLINFEISTLQSEIVDNSGSFSINYIKPLVLAPIKSYILGEQTILQGFTSTSINIEQQWQYAIYRSIAFFVEFDLIIKSPSYTPLFYLDDGSYGIVSGTIDSYKVQFNIVNSKYDIRSEVSYYFLNSLNNIAIIPSPLDLFGPIMTNISSIPMPNGFNRYFNIRFSVVDDKSGFRMLLFTSFYGNEITRSIFTNSLLKGSCIDGTYEVLVDSNFYYAVTFYIWDYASRLNIFGSTPTSEKQVASMMTSNPGGEIPLSCLNDLTSIGWSHNDIDVSSVSFSTKLLFNISNADKLFSPSLNFVGYDNLPLETFYGYWNETSEQYEIVVTIPLRAFTGVVKYNLNTKCSRASAYESPFLHSLFPSSELRVSSNDADMFGPEISKLVQIPGQTAIISAGGTNIGWKLDIYDRLNGFESGNITIVSDADLTEYTFVLDPNNQTQDISIKIKEKCVSQSYSIQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL08</accession>
    <name>A0A8J4PL08_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008887</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000594">
        <property type="protein sequence ID" value="KAF2069792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL08"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550:SF10">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="757" mass="87553" checksum="C6FD8238C0825BDB" modified="2022-05-25" version="1">MNHLYGSVFGNQYLRKKIYSLIYVLQHYDNSLRYSDIADIRYMIKYGHIGLLKDKVQRQELLFNSGTVVDLYPLIDRDLELFKTLYHTHKATPTNRSLFFEDVIAKNNFECVRWVCESGLAYEVTDDHFKFTTDSDPAILDYLITNKWYTVTHESVLKIYKKSYSPFSPSILQVFKQHTPNPISSEQATAIINSLLSVPIPLVFETLLPLFQKDIQVNLDSRLTINIRSLSTLPILKYLLDNNLAKEKESMVHEAIKSMDTLVNISAFIDQIKQISNNNSNSISQLLTKFFPVQFTKEDNDRLANSFCDELYSLPIDALHALIDHGCKLPWFNNLVKMGNRDALFSLWRRFQTLDQIKFIGNEITFQPDTTLGNQQKVYIDFRNAMDVHIIEFLLEQGYSPDTIKQYIRVKPDSIDIIANQRHTMARELDLLIKHINQGIIQCDSILSICLERGNNRLFKSIYTKTQDKLSELSVSQLLKRATALQQLEPLSLLQLQSGAKPLTIAQINVQNPKLSYIKSVMASHPELAEQFQGMLKNFIIPNRYDIVKYMLSTFHYGALGSDIMALLSPSNPAMVDLFYRHQDLAFNTHSQWEDLIVQCASLPNHQSAHLIQYFVNNKIVTSISNELKSLVILQPPTPVLVAITPLLDPVRHKRVLEGKSFFDHRDLFNCFMYLIDNQVYDTVTPYIDLIVRDAIKPIKFWNPNTLCRCLEYLIKNQHIFKNPSNPKDIINQIQSHPIVDTTLLHYIDTHYKNQYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL13</accession>
    <name>A0A8J4PL13_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Autophagy-related protein 27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">CYY_009880</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Preautophagosomal structure membrane</location>
            <topology evidence="4">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ATG27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000841">
        <property type="protein sequence ID" value="KAF2068795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL13"/>
    <dbReference type="OrthoDB" id="20652at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018939">
        <property type="entry name" value="Autophagy-rel_prot_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15071:SF37">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15071">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09451">
        <property type="entry name" value="ATG27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0072">Autophagy</keyword>
    <keyword evidence="17" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 20" id="KW-0732">Signal</keyword>
    <keyword evidence="8 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035158087" description="Autophagy-related protein 27" evidence="20">
        <location>
            <begin position="21"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="21">
        <location>
            <begin position="25"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="163"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="185"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAF2068795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="275" mass="28887" checksum="805C69CC0E8EF87B" modified="2022-05-25" version="1">MSKFLISTLIAIIFIGAISAQSAPSNCMTTLGGKKYNFSGFGNSTSWSFVVAGTNPTTYYWNVCAAASKCNSIKPTTPASCQFQSNTYRNTGDLASGSFVNYNAGSSGGGAIKYTTTGSPCKSGNTRTTTVQMTCTPGKANIVTGADENPVGSCLYEVQLSGKSACPTTTPTTSGTTTGGDDGSSHSQETSSSHSGEKPKKKGLSGGWIFIIILLCSITVYLVVGVIINAKVRHMHGTDMIPNKNFWVEFGALIKDGVFFIKSKITRSNSGYQQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL34</accession>
    <name>A0A8J4PL34_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000753">
        <property type="protein sequence ID" value="KAF2069084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL34"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="437" mass="48139" checksum="080BFD55BCF4C02A" modified="2022-05-25" version="1">MTISRINHPEDIPNIFGALKILSHDTIEKEQSNLVARPEADCLSADNDTPTEHEVAPCKTPQILTTKENRAAYQTNNWAKINEIAQTSEKALKPLYSNIASKGDDAWTKVKSSNGNEFCMTWNDEAAIWAYTESITPSNEAQLTENPKTTYQAVVQIGTYVKSKTILGLHSYNFGIASLIGTSIASLLVARTISTFIAEGLGFTTASFAARMTSIAARIGIRNTVFRIAARALSALGSCILFAVVFVGISLLWDWLNRLYTVQIQILNWDNSDWTVGEHYESNAKIPGDEAYGPDFNKKIPKMVSAGSVVTPPDFGPIKTLDSICYYALIVYQNNSTFMEGCDAVINVKCSDYTDSQNQGFTYGISVPRFKDNKHAISNGIHNGQSFIKDESQWKSEKGVSTHWNGKQITASLKDLSGAKDNFYDVIININNKPVGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL79</accession>
    <name>A0A8J4PL79_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Leucine-rich repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_009705</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000784">
        <property type="protein sequence ID" value="KAF2068973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL79"/>
    <dbReference type="OrthoDB" id="19198at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454">
        <property type="entry name" value="NISCHARIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454:SF68">
        <property type="entry name" value="PH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="490"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="490"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="513"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="545"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="563"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="574"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2068973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="603" mass="68805" checksum="7B7C50C14F256A4C" modified="2022-05-25" version="1" fragment="single">MGKKDQVATQQFTNSDDLLISLNTFIRSNETKLKEGTVFLTLNPTCLEYLNLYLNNRRVSFTLKRTDILDLQFILTKVQHLRLDPNIPDKLEKSKDINLTTFESLKILDLSYIKPALITNLCSISKHLTSISIHFSLYSLDELLFVEDETTLPGYETTIHLDFEDVHDAVEVQKKRFQALYQRNKIWSKLETLNLSFNHIPAIDYSIYSLTALKSLNLEGNLIQKIENTQECMSLRELNLSFNSIQLVSHVYEIMGSVAQLYLRGNLIENLDGLDKLYALELLDIGHNKIKQLEEIFKLKKLLNLESLYIDDNPISIIKSHRQLIFNSFFEDNINSIDYKPFYLNGKKANESEIEYVKKHNDHSASSIKKIKPDNTNKSSTPTPVVMAPDAIIHHKHNEQHGSNIYTNGNHQNGNGKISSSPHKHKHAFQRTVDLEQAVTIDNNTYEEEIPIVKPLDDKKFEEEKSLKKKIDEIRLEGGNSWLLILNEMNTSNPSSTSNSTPSTPTKRYPNSTTTTTTTTTTITSGNSSPFIATPKKLSPEKMAIRKKTRKLKIRKEEPEISNPMTIPSSSSPNNNNNNNNNNNNNNNNNNNNNNNNNNNNNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLK9</accession>
    <name>A0A8J4PLK9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MACPF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008497</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000529">
        <property type="protein sequence ID" value="KAF2070182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="21110at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MACPF" evidence="2">
        <location>
            <begin position="1159"/>
            <end position="1509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1261"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1403"/>
            <end position="1429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1912"/>
            <end position="1951"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1261"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1410"/>
            <end position="1419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1916"/>
            <end position="1925"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1951" mass="220351" checksum="3231088DB2A05DD3" modified="2022-05-25" version="1">MGTTAAVVASPLALVPNDIDMLYLDPDTLEWNCAYFSTSHSPINTIKTKESIFENKYPYGVISKNNLKFFYGDYCTYGENLYFLSDDGTSWVTKPIEFRGPIAVDTTPPQLVHSDFIIVNSKIFYIRIQVSDISGSGIYRFECDTYENNFFYAYDTLASGDFQNGLFDLVFHINEINHIRSTGLVIYLYDYTGNRSPPYIVSPNNLFDNDTFPSPTLTIYQTDQGIDPQTFTGEYHQGLRQYSIEFEIPSTIKDGILEYTIGIFPYHFTSSELINALGSKASISINSNNVPAPVLTSIEFLFTNNDVGVFYNYHPIESYFNLRIMSQNGFKNGIVYFTTEFDQEPIKVEFSVANLIMDHMGGPSDQYNGLFQSFFIISEVCLEQNYRISKIDLYDKNEQYSTWPPVDGVENAAATGFIPSNPSLRFECFNPPLSDNDPPTISSFDSILASDASSIQVTFTVDESWGGYGISKMHNPYIYLTSIYSEFRQQASIDTMDSLFTKSYKTTIPIPSEMIAFGFKLSIYGLTDGKLNKNGYRSIDLVEYLNIDQSKVFYKSSLLVRPTITSLSSYNGYINGHLTIYGKAFPQDSKVYVKMDQDFQYIESVVELSTLIIVNLPPFSGEISVRVEYSQNQVTRTTNTMTTAPVKISTNYVYVDTSSMCTEGCGTHDKPFGNIQDAIDKTDGLKYTIALLPGIYKGLKNNDLNIKDSIVKITSLFGPDSTIIDCEGFGYGFNVDNSKRFSLSEVTIKNCTSDKGAGLFLRNSRTLLNNVHFIKNIATNGGAIYVFGKELIINDCVFLNNTGTYDGAAIFSVASLVKIKGDLTHFIHKNNIDASSNVDIIGQARDILCKNSTIKIDQTISMDGVDFKCESGCNSFYSTNLCIYTPVEYLASPTTPLCTTHSCLTRPNECSCYFNGLLLETFKDNCHYNSFESCFVETKQIIQNAYLDNLMSGVSNVVSRISGYISIYESRYVLFLFSGTNFGFSFKVNGLQVFSLSQSTRFNESTSIYLIDQHIHFIEIIIFSSSISEKQFSMNPILGVDDELFFSNLICGDKVLNHVPNSNPKNGCDTIKAEDCPPDSGAIKMKLSTNNLMSIMKPVVCEQETLVEKFDRCGDGVCNEDPNSCFQDCYKQYTKTCPARSVPDGHIAPGFFIEQDTLGDLISNQFLWKLPGSDHLTFGIDIIDASEAFSPVFQFDYCGDVAQNIIEDAYRGNVYHIPDQLNVKPMPECTFSAKTTSYKSTLEIQREMAENSARDYKLQLEADSSKSKDPPPEDEAFSLSLGISHSNEKSVEESSKQSTTEKERIFKSDVYCKTSFVELDLDRISFNPNFLNDLNQVKGLEDMLILIQRYGTYFYKSSFLGGKLSQLTITRETNVENSSENSWEEKSQTSMSLSVKTPMFSIDGGGIEESLDKKNKESTEDSETTESTTSKVLTYGGIAAAFSPASDGYSSPTYDQWVESIDLLPVPIDYQLFPIKKYINPSWVNKYNQKLSELWTSAEKVYYILNGFYREVDTPYSIIFRLDKNVMPNNVLVPGNPLLQITYWPTLDKAVTVKVPLPHIHIDTLGKKHSLLYISDLDEFQDYHCRDYQAFNRDISSQFSYTATSCDKGSDDITSDPIRYDIDLPIDLFSSKLEPQVSLSFKYNNQELILNSPNQPSKIINWDSGNAILFSSNQLVKTTTTFATVAFENRWAWHLSPTYFKDRKMEFSKASFNCEGSSCLDTLSFQLKYEGFGIGDDISSKVFSTFNLKQKNQYIHWQDFTFRSADWESFDLTHQSKIGMQTRVLWMKAYTLDETVSWHYNIKSYHIKTDQKTIPAEEFVEVNNDGLSYDRFNSTRNYKFSKSLEYKQLFTYHNYNYPTQGVKAETIYDIFTVKRYGTVLTNGLNSPFVSMTEKFYYNNPEGDLDPIQDIPVEEQASPSASASASGEMWDGGEENDGPQLSVQGNGFWKPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM86</accession>
    <name>A0A8J4PM86_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation of fatty acids protein</fullName>
            <ecNumber evidence="10">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_009072</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>an acyl-CoA + malonyl-CoA + H(+) = a 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:50252"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:50253"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ELO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="10"/>
    <dbReference type="EMBL" id="AJWJ01000631">
        <property type="protein sequence ID" value="KAF2069607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM86"/>
    <dbReference type="OrthoDB" id="434092at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF104">
        <property type="entry name" value="ELONGATION OF FATTY ACIDS PROTEIN SRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="199"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2069607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="263" mass="30643" checksum="85B3F43079A693C8" modified="2022-05-25" version="1">MEYINQIDQEIENFRWVSNVTPFSNWKWPAATAVVYLVVIFTLKTLMKNRKAFSLKGFSIVHNINLIILSVAMMTGVLHAAYEQAKEQGAFSLVCEKTDQAVQGRIGFWIYVFYLSKFYELIDTVILALKKRPIIFLHIFHHMAMVPITWQWLNDQWLVGSWWCVFVNSFIHTIMYYYYLQTSLGNPCWFKKYITKAQIVQFLTGTAMVSYWFYIRDSEGCKGSLAPAIVSYVVNTSFILLFAKFYLNSYKSSSSADKKSKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMG5</accession>
    <name>A0A8J4PMG5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008912</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000597">
        <property type="protein sequence ID" value="KAF2069773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMG5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="143" mass="16397" checksum="F621D4DCD51F84B2" modified="2022-05-25" version="1">MYQCLLNLPDDIPLTYGQEGIIFKNNTQRSMTTHFNLIARIQFDRGRNTLFRFFARALPINHIRLKLCTYNCGLLEEPYVDPLIDCPVIPDTTKTNAIKFINVLSGTRRKGGHIRSKNKKKEQNSSRGFSLISRDVRIAVRGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMW3</accession>
    <name>A0A8J4PMW3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_007844</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000441">
        <property type="protein sequence ID" value="KAF2070840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMW3"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003035">
        <property type="entry name" value="ABC transporter AbcH.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015854">
        <property type="entry name" value="ABC_transpr_LolD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24220:SF86">
        <property type="entry name" value="ABC TRANSPORTER ABCH.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24220">
        <property type="entry name" value="IMPORT ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="11"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2070840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="267" mass="30040" checksum="7CCFEA9B689E33BC" modified="2022-05-25" version="1">MQKKQFDEVVVEVKHVSKHYKIAGREEQVKALNDIHLASDSELLPIRRGEFVMLRGPSGGGKTSLLNLLGTIDRATEGVIELFAHPISVDSTDEYLAKLRLEKIGFVFQTFNLLATMSAYENVELPMIILGKLSEKERKKRAIELLTLVGLQDRLDHLPSELSGGEQQRVTIARALANSPEILLLDEPTGDLDTKNTVEVMDLLLDLNLTLKTTCIMVTHNPDIECYADRILYIADGVFEKQVFNIEQTRLDYDQYIVYLNSQSGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNC2</accession>
    <name>A0A8J4PNC2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phytanoyl-CoA dioxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008274</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000496">
        <property type="protein sequence ID" value="KAF2070404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNC2"/>
    <dbReference type="OrthoDB" id="14800at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008775">
        <property type="entry name" value="Phytyl_CoA_dOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31630:SF10">
        <property type="entry name" value="PHYTANOYL-COA DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31630">
        <property type="entry name" value="PHYTANOYL-COA DIOXYGENASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05721">
        <property type="entry name" value="PhyH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="310" mass="35967" checksum="D2D38655A42FD631" modified="2022-05-25" version="1">MTTNPFLEKCNTWNENWPEWRKNLYNNGWAIIPNLVAKEKAAAYKEEFWTWVEQNRNRARRNDPSTWTEQNMGSSIYRGIIHDEIAHTQWMWDIRSEDAILDVFTQVYGTDKLLVSFDGANISRPLEEKEALESWAHFDQGHLKYGFQCVQGLLNLEHCGETDGGLVVLEGSHQFHDEYFKTTGIETESDWYPFEDEDVTKLDLFKNCKKIKVCCKPGDFVVWDSRTVHWACSPIKGPNKKCRMVTYVSMQPAEIATPETIEAKQMAFKTKRNTSHWASTNIDFFGIERGQVNNIPLTPRIEKLAGLVPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNM3</accession>
    <name>A0A8J4PNM3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008203</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000486">
        <property type="protein sequence ID" value="KAF2070478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNM3"/>
    <dbReference type="OrthoDB" id="24468at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32142:SF55">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32142">
        <property type="entry name" value="B BOX-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="627" mass="74232" checksum="4462D0597C5C01C9" modified="2022-05-25" version="1">MNKNLYTLVFNNIYISNKIFNTVHQLQLYNNSLKYNDIVYVDWMIENNHIGLIKDKLKRNIQLHVELEYLFTTVANKSTDIFIGLFKANKYRHLKYIQQVGLQELITNLDNVDVAKYLFEQSFIKELKILNFHQLYCIDTQVLQYLVESGYCKPTFESLLLSTSKGINMYYNPTKLETTRNKIEIILNFIQTIPLSNDHSTTIINSLLKNPPPLVFDSIKKLLDQRVVRPIAIKEIKKKETEYYDYNSITPILPDEIQSIKDSLPNTKLDLLFIQKLYADMELLDWYSCIGKSQQEFINLWNRLESKIRVADSTIYYNAATDIDPTMTDRDLAYIKNVKTIHQLIFYIISNVETKDPLKLVQFLIEKGMNNFMYIAGQIKEKICHEILKNQTIDQKDCETIYRLAKHDSQFTYDVLSSCCKNGHLENFNYFFKRFKNRNIDFIVHLLENTVLFKNLQLYHKLEEMGHYFSDYPKECRNHFDAQSFGKTMLSNIDRIIGNVKKEEEKRDLCCTLFANSIGRNDFISFKYIIENHFQWISRDFNNIIEKKYMFNNLAFIGYLSNYESTLFNVETKIMFFSNLFNLAITKQYLPLAEYLIQNNCIKDITTQHRKSLEHPSFMDLCNIINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPB3</accession>
    <name>A0A8J4PPB3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">BEACH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_007734</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000429">
        <property type="protein sequence ID" value="KAF2070943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="26681at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016050">
        <property type="term" value="P:vesicle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06071">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01201">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000292">
        <property type="entry name" value="Lysosomal trafficking regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1540.10:FF:000002">
        <property type="entry name" value="WD repeat and FYVE domain containing 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1540.10">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056252">
        <property type="entry name" value="Alfy-like_Arm-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036372">
        <property type="entry name" value="BEACH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051944">
        <property type="entry name" value="BEACH_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023362">
        <property type="entry name" value="PH-BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46108">
        <property type="entry name" value="BLUE CHEESE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46108:SF4">
        <property type="entry name" value="BLUE CHEESE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23295">
        <property type="entry name" value="Arm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02138">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14844">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM01026">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81837">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51783">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BEACH-type PH" evidence="7">
        <location>
            <begin position="2662"/>
            <end position="2787"/>
        </location>
    </feature>
    <feature type="domain" description="BEACH" evidence="6">
        <location>
            <begin position="2820"/>
            <end position="3118"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="3236"/>
            <end position="3277"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="3389"/>
            <end position="3421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="628"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1283"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1729"/>
            <end position="1749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1801"/>
            <end position="1820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2500"/>
            <end position="2552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2570"/>
            <end position="2637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="38"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="628"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1283"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1735"/>
            <end position="1749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1804"/>
            <end position="1820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="2503"/>
            <end position="2520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="2532"/>
            <end position="2550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="2579"/>
            <end position="2625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="2626"/>
            <end position="2637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2070943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="3438" mass="387714" checksum="FED20FE900B4BFF3" modified="2022-05-25" version="1">MFRKMKDWVKDLPLGVGGEDQEQQQQQQHAQIQQQQQSAATASNTSSPKYTSPGSISSPPSRNTSIDSGLSSATTAADRSNNNSNNNNNNIYSLIKSEEIKDIWSVMTGSEVDTVRSSSNTQKLKALWTDFQSSKSDKDKILKLNKLLPHFISLYEDKKIDVKNMLNDIFGNNSKAFSFAVSRRLVKDLNEIWKKHSTAASAQQAAAGSATNPKEAVAKEIYGFLSTTGGLVCGFELLYAIEILCESPQSCEAMSEASVPSALVRCLQAMFNVPVGILESHKGIIQDKLIKSLCYLSRQKAAIEELQKTDTLSALFSLMSHNCAPTHRSLRAQIGSFGHELTDLHPPTITYINSKKVIPSMIADLNNYYNYTPETYISLCKIIIKILSESSKKSAILLDDFLKINGYSFFVESLFRLESSKDKPHLFDLLLDAICSLIYVGYGHISVPVLDQSPVPYQSAIQNLKDLSNQSNISKNEHAFIILERYFLKSTYEENRIKILDRILTVFSSNPANFILLQHCNTITKFIQEYESLNNNLKHHVMKIVCFVVTVLSCVPFQELSTFSLLVNENPSQFTLEMVLQLITTLVNFEFRYKHIFRESGLLDILVKVIDTFSQDVIRLNNKYVNRNNNSNNSNSKNNNNQPQEDEEELFESNIQVKSLEILLDCLYLLISEHPDNIALVRSFSLFNILLHFLPYNSVRGKCLRILQQLIKYDNDPSQREFDGLIKVLTSLNKDNYSMKTDILNSIRKLFLMSKHSTDSFREHGGFVSIISILIALESSFSAEKISAMKGKSQIFDQLELIEAICRTTTAALSGNVLNRESFEQQIGYSTFASCLIMTNVLSTEYANSVVGYIFDMVTENLSGGDYHLSLSSSKAANSSSTALTSTQQQNSIINNVEAFNIILDIIPHVSEHDFVLAIIRRINRMAEFGRFNQEALSKLSIPDWVLQKFPSNLSNAKDPLQPLLLNLIQTVGANCLSGSELRQFVKLLQPENSPQVLLKILSSMAKSPPSPPYFEFNITKNPHAYIRLPIQDRTWPPTNGYTIMFWLYIEKASTQNVVDLVHIYSDDKKSALYIFIKNGFLYVHITNNSKYVIEVQSFKFTESKWYHIGIVHARRLLSGTDFKLFVDGFLKYTATKAQYPAQITSGTGLFCDIGTSSMNRFPVEQIWRIGPFYLLEESLSAKHINTIYFLGPNYVGNFKGRFSPYQTYEIVNSQNLLAIKDLDYGDQLGPLNLAKVSMSIDENRIVVGLCASNKIIRTNNSPKIVYNEILNHILNDISQANGSTPTMMSSNSSPTTNSGGSLKKKEKDFRVEIINQSDLTTKQRGVLSGSVEAFRRNKVADSIKKIGGMPIALLLLEKSNNVATLHDSLSLLVGLIQYHPTNTHEMSLINGYELLAWVLKKKVDLFNIDIIDLLFDFIGINTHLTRNRSEGTVANWNACKYIMMNYEIWKNTSVQLQKYILDGYNSLIMGNVQKKFNIDSLRKIHLIQELFDILSDESVPEEVASETVITVLYNVLSEGGLIEDDIRQVSAFLIAGLHQGQQQQTSSKRKSTTGKSKHTTVIADNSTRTNRLVNRVFYIFLQVCANCQDPAIIFRRVSSFWCFYFIDDIHPPLTVSLALRVTSVFFLLKYEYCASFIKKSGFKLLEKIVPALSGYQEFYIIFLHLLLGSDPKSLLDISLQSPTLEFHELLSMFKPVEKNLFCIESAQLMLAMVKKSYEDNYKLYQKQQQERDISASSNNNASNNANNDNEEFLSSLMSASSLNQSSNSTSPLSFSQGISPSASNSSISSLANSNASVGNQSINTTTTTTTSVSNPNTPSSVFSRVGSFLSKVEEKTTGFVAGALEEVSDGQVQISTKKKRASVSITSSSLNNGNTNSIGNLPTSGSNSNLMNLFLANGSTDNADNSGNANNDGNNNNNVNNNSFLYLSNFASPEGATSLQHTILTYFIYLFHENHHFQQECFSYTIVEYLISILFPKNKIHLPPISTVALGTISSGSIGALGGSNPLVKDRVLDLVLKFLCQIMLSALRKTSKAIGIVEMILEGTPHTISDDDFVLYHTRIIIELMVVIETNITKSEYYDNERVHSNINKLCSMLVDRIQLDLLVRSHKTLISRRLFFFIVKVLEKFEADRMSSKSAQPLYSSLNRAILYLFNNLADSPVELGHIINHIINHQRIIFSDNNIDLEYYQTLCYLNYKSITSDQQESVDNGLKMFKLLLSLKGSNYFEMMATTLQLKVPSATNPRGTEIIDLKSGFEMVASSNFKEFRVWLSDNLAQVTQVFEESPKRAYSNQIGSEKKNANEHTFVNMKSRRKERLVRMDRSERKEFLHEEEKFNHITKKAQYFVAVDQERKKKIRQLENDKQKFNAIQWENMRAQLTRERAVWGPSEPSPLDKWKMDSTEGPYRMRKKMEKNYSFYKDYPYIPPSFDENNNSLPIPCSGDSETFMNLVGTELEYSLDPSYWKYDLISTNQVITSSTNVNSNVVNNNNISNNTTNTTITVSTPLKSNNDSSPLAKSSTGDITPPQSPIQSSSEDHQSLSMMKREYSKDSLLESEEAAINSMKESELFGSVQDETSVGGQQQQTPSSSSTPQTPSIIPTTPSSQQPNIPTTPGGSNNNNPSSNPQNEVDEEPNNNNTNEDETQAFIRLLDPSDQKQLREEMRRDPRLPYTMFNVGSVEGMDKIEGILIFCNNYMYIFDGYSKDPANGEISEVEEKINSEWLPEGTVLPQKKKIIHHFIKWSYDDIRDVLKRRYLLRQVALEIFSTDGRNNLIVFKDEPTRDEVHNNLLRHLADNNYTIGGDASGISGSQTGVDEQDLGVRDRLTSIWRKSPLTTKWQQGQISNFQYLMHLNTLAGRSYNDLTQYPVFPWVLVDYESDYLDLDDPKVYRDLSKPMGALKEPRAQKFRERFENWDDQEPNEHGHKVPKFHYGTHYSSAAIVLYYLIRLEPFTQHFLKLQGGRWDQPDRLFSAIEEAWGNSSCGSTGVVMELIPEFYYLPEFLGNSNKFNFGTKQGGESIDDVALPKWAKGSPEEFIRLHRKALESDYVSEHLHEWIDLIFGYKQQGKAAEDALNVFYYLTYEGAVNIDAITDPVEKAATIAQINNFGQTPKQLFDKPHPKRNQNLNQFPFYAKPLVGNFIKDINEPVGQIRILNDRATCVGFNKVLLPPSWTKYVLWGLPDGSIRYQNGDKIKVLEDHHDGPLTCMTSTEDGRICVSGGTDSLICVYNINNRFSLAKRLVGHTAPITCVVASRPYSVIVSGSDDSTCIIWDLNRLTYVRSLVGHEGPISCVCIHDTTGEIVTCSGTTISIYSINGVRILSHKTSQITSHDQITCCIWSKGPEWLGENVLLTGHRDGKIKVWGIESRLVQSETNKDQMVYKNIIVLRSTFYNQSAHNSAITSIHLTNDQSKLYSGDIEGRVVMWSEEPTPVKQRGWTLGGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ51</accession>
    <name>A0A8J4PQ51_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007881</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000445">
        <property type="protein sequence ID" value="KAF2070790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ51"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="77" mass="8780" checksum="F13A3717446AAF18" modified="2022-05-25" version="1">MSKQFSHKEYQEFTDKLENPGPAKVQLSYFRGDYDCRSPMMVSTDPKSATSSSSKLSKKKLLFQLNNLNSEAIPMNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQC4</accession>
    <name>A0A8J4PQC4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ribosomal RNA-processing protein 44</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">CYY_008494</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the RNR ribonuclease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000528">
        <property type="protein sequence ID" value="KAF2070189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQC4"/>
    <dbReference type="OrthoDB" id="372421at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071034">
        <property type="term" value="P:CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071031">
        <property type="term" value="P:nuclear mRNA surveillance of mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09862">
        <property type="entry name" value="PIN_Rrp44-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000010">
        <property type="entry name" value="Exosome complex exonuclease DIS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.700:FF:000001">
        <property type="entry name" value="Exosome complex exonuclease exoribonuclease (Rrp44)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000125">
        <property type="entry name" value="exosome complex exonuclease RRP44 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041505">
        <property type="entry name" value="Dis3_CSD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022966">
        <property type="entry name" value="RNase_II/R_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050180">
        <property type="entry name" value="RNR_Ribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033771">
        <property type="entry name" value="Rrp44_CSD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033770">
        <property type="entry name" value="RRP44_S1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355:SF35">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RRP44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17849">
        <property type="entry name" value="OB_Dis3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13638">
        <property type="entry name" value="PIN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17216">
        <property type="entry name" value="Rrp44_CSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17215">
        <property type="entry name" value="Rrp44_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01175">
        <property type="entry name" value="RIBONUCLEASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="10" id="KW-0271">Exosome</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="PIN" evidence="17">
        <location>
            <begin position="67"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="RNB" evidence="18">
        <location>
            <begin position="463"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="937"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="937"/>
            <end position="950"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00077930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF2070189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="991" mass="112535" checksum="03B39EAE74ACAC16" modified="2022-05-25" version="1">MQSNKFYYRTTKRGNVIKVVNEIYLRDDIPCGHDKCTECLEMLNKRTTSEERTILAGQGINQLTEDKVLLLDTNVVLHQIDLLEDPALKDIIILSTVLDEVKNQNKSIFNRIRDIIKEPTRRFYVYSNEYSQYTAINRETGETPNDRNDRAIRHATEWYRSHLHKYNIKIILITNDKENLLKAKSMGLDCQTIQDLVFEKSNDNPSLVDKLSDPSDENDQDESKRFSFTEHKPISLLSEEIKNGKVFQGIFRTSMTNYKEATVQSNTLEKDILIQGIENLNRAVDGDIVAVELFPESEWKTPSTMIMLENDGVEEDDDSIAKDAMVAHLQPTGKVVGIVKRNWKPYCGAVEFKGDTSKSTGLHFLLFLPIDKRIPKIRIKSRQVSNLVGKRIVVAIDQWDKNSKFPSGHFVKDLGPLGDKETESQVLLLQFDIAHHPFGAAVMKCLPKPDIMDKVGASDMVGRRDLRGECIFSVDPPGCTDIDDALHVKKLKDGLYEVGVHIADVSHFVQEGTAIDEEAQSRSTSVYLVDRRIDMLPGELSGNLCSLMSNVDRFSFSCIWKITEAGEIVNVEYTKSIIRSKASLTYEQAQIRIDDKTQNDEISTNLRVLNSLAKILRKQRFDRGALYLASPQVKFKTEELGGDPSDVEIYQLRETNAMIEEFMLLANIWVAKKIHKHFPGCALLRRHPTPKKSSFDLLTQLIENKGFKFLTGSSKELAESLDACTLQDDSYFNTLCRILTTRCMSPAVYFSSGSLPIEDYHHYGLATEIYTHFTSPIRRYPDIIVHRLLASAIGMQSVSLNLENKTISGLCENFNFRHKMAQYAGRGSTQLHTLIFFKGRKCVEDAYIIRVKANAFVVLVPRYGFEGTVYVSDPNSTSCSFNYNHDDQTLTNGQLTFKVFDKVTVEIFVDDSKAHDQKLKIECINPKITFTIEQENQENTTNGNQQQENGKQPIKPIVKKESKTSAAKKTRKEPDTTESTIKVNKKVKSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQJ9</accession>
    <name>A0A8J4PQJ9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">inositol-3-phosphate synthase</fullName>
            <ecNumber evidence="6">5.5.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_006443</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glucose 6-phosphate = 1D-myo-inositol 3-phosphate</text>
            <dbReference type="Rhea" id="RHEA:10716"/>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="5.5.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the myo-inositol 1-phosphate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.5.1.4" evidence="6"/>
    <dbReference type="EMBL" id="AJWJ01000297">
        <property type="protein sequence ID" value="KAF2072243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQJ9"/>
    <dbReference type="OrthoDB" id="2887at2759"/>
    <dbReference type="UniPathway" id="UPA00823">
        <property type="reaction ID" value="UER00787"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004512">
        <property type="term" value="F:inositol-3-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000107">
        <property type="entry name" value="inositol-3-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000069">
        <property type="entry name" value="Inositol-3-phosphate synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000055">
        <property type="entry name" value="Putative myo-inositol-1-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002587">
        <property type="entry name" value="Myo-inos-1-P_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013021">
        <property type="entry name" value="Myo-inos-1-P_Synthase_GAPDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11510">
        <property type="entry name" value="MYO-INOSITOL-1 PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01658">
        <property type="entry name" value="Inos-1-P_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07994">
        <property type="entry name" value="NAD_binding_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015578">
        <property type="entry name" value="Myoinos-ppht_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0398">Inositol biosynthesis</keyword>
    <keyword evidence="13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="14" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myo-inositol-1-phosphate synthase GAPDH-like" evidence="15">
        <location>
            <begin position="311"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2072243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="512" mass="56798" checksum="B7A885943CFDDE05" modified="2022-05-25" version="1">MQSAQMYDTFRVNSPNVEYTEEAIISKYTYQTTKSAFINNELVVEPVDQKYTFKTERKVPRLGCMIVGLGGNNGTTVVGGVIANREGLCWKTKQGVQSPNYFGSVIMSSTIKLGLDEKGRDAYVPLKNLVPMVHPNDIVFGGWDINNADLAEAMQRAEVFEYDLQAQLIPHMKQIRPLPSIYFPDFIAANQKDRANNVLTGTKKEQMEQIRKDIREFKEANKLDKVIVCWSANTERFSSVIAGINDTWENLQAAIEKGEEEISPSTLFAVASILENTTYINGSPQNTFVPAVVELAIAKNVTIGGDDFKTGQTKIKSVLTDFLVSAGIKPVSIVSYNHLGNNDGKNLSAPQQFRSKEITKSNVVDDMIASNSILYKEGEHPDHVIVIKYVPYVGDSKRAMDEYTSQIFMGGHNTIVLHNTCEDSLLAAPIILDLVILAELSARISMKPEGSDEFIGFHPVLSILSYLLKAPLVPEKAKVVNALFKQRAAIENILKACLSLPPDNNMLLEERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQT9</accession>
    <name>A0A8J4PQT9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000281">
        <property type="protein sequence ID" value="KAF2072430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="16716at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="2430" mass="275721" checksum="7750C040646A2176" modified="2022-05-25" version="1">MSPGLPTPDFQDVAQNTLRDEILDLLEKNKVKYDQSIVGVVSYVPLFIDFGSFINYKPSDRATVLKNIKANIVNIIKQVVASLEKMDALNKGNVNSFMTRIVFQNIAGKASTSKKSTFYPDGTLEITTIFEDGLNCQYYLEKKIITALNEFRPMQGAVVPDKVVAFVPEPVAPPQPEPVVAAPVVPTTAGLDFSVPTYANGAWETLKLEVLKVMVPLKIDASVLPGAPFELPVSVDWSSIALSGADPSSILKSLKNNIPAISKQIIPGIQKIDEYRKPMIRDYMSSIVFQNIPGKASTLKRAVLTPEGSLMLIVPFEDSLNCSYYFEKKITTGLCEFSPVGASAPQPKTVISAPVAPQPTVVAQPAKPNVPQYSIPQTYQPQQPPAYTPVVQQPFVPVSPNPAYSIPQTYQQQPPQPAYVPVYQQPAVYQPPVVVPQPVAPPAPAVVYKEPPKEKGRPEMAERPNIPLEDATNQFYLREIETFVKTFEVIDEKDQPIGAPIYIDWSSIMVYPSPLKQNTVFRNVNLALYKILRDCIAAIKLACTDYGNRDNIVEYLRSFTVQNVQGTSSTAKKVVYEDGHLTLQTTFEDYGNCGLYIDSKVVQALGSRPSAPPAKKPDAATIAKEEIIVRLKSHQSIKNKDNQTYTPSCPIEMDWGYQSVKAYDGANLEVNFNVITNGILTDMFIAIQSFPAKEIDLILSEIKKVVFVQATPGKTIFDKRTVLLTDPGTLNITTTFEDRTDGLKSFKEKFTNCFQSRIYKVEIRDVLVPALKNMIDTAVKVDTKPNPLDNYPVDEYKSYSSAVVFDWQIVEKESLESQVGIYKSLVSTLRTESTFATLLNKAIRDICSYDVGKNAFLPCTTFILRNIMRHKEETTKQTTPNTWEFFVSFHDLTKYQITQKSLTTLFKETLKVAYPCAIQDCFPLVEEMTANLESSTGKNVPVIMHYDSWRNFDRFKNDATSYRSIDNVVIKLPETGLACIKTLCKDKIVLKEYTRLVNQIHIVYDTTDSVKGHRSQDPDVVAKLEKGILTFTCNFKDGKMGHTRGWDYQLELVLATRPLKVTRAMEKAESALADVSQQLGALIGKPGVKVTVDYQGFIHEYNFLLALEEDVYTKVIMFFSNAIQRGWISDSESSDLSSYETVKKSIQSQLTVVEFGVNVNNSQKEHYDFTLANGKFRVNMNLSSCMKGETKSWRLDLERIFKLRDLKLREEIAKRIDISRSKQGIAQTVGKPIEIQIDWESLINNPDFSRNTVDYITYIHKFSETILAEFPKPSGFGRVCEYQEVQTYVQQNLTKINLVAGSSSRGVDISYDKSSKSIEIAVGIKNLLKNVSSNASDYEEYGRKIENVMALRLMMVKGYIQRNQSDQLSSASSRITEAIENDAKVTIEWDTIIQHSGFLEIVEFKKPLEHIIDIAHELHTIVQLCRENHQAKALLKPVRQWTIVIDGRDSINESEFDDNKYGVDWAKVSWRGLPAKEHIQVTVNLEQLTRKPHNLGLHLKLEFLVTPDKALQRYQNKKEDQYRQDKLEQDARYHDQQLRMDQRMLDASNQRNRELDRLNRNLHLPFFKISNDDFIYTPLPRIPENIRYIKFSKCSFSQLLSLPPHINYLELYIDNWINVAGFIPKSITHLNIRKNNSLSIKDFLSTATIYLPSTLEQVFVEGEDFVKRIKIVKQFQYYHNNVKFKSQLKKLVLPETFSTFIPPNSLPPSLLELRINDMHARELPLNALPHSLEVFDIRYISSVNVKSSFPSTLSSLKIVTDSLDNIKMNTEAIKELDLYTRSAKICKEMKNVKKLRISTINNQELISVFPSLEDLTIENEINNSLIIPRVKNLTFSNSFGKDIVIPDSVKNLKVGKETSLLSVQIPKSIEKIGLNFSINQKLLLLNNGNLNNAESNDYSSINHLDNHPFIYIWRNKYLKSKIINSLINPSTSKFQTPLIVNNNTLFINDLLKIKQIIGGSICLKGIKEITTTNECLSTLGCQIPYEISKLFKEKVFIYQEKDNNENTEIPFDTKVLVWNINQAIPIGLVRCGVNTIVFGNSFNQTILKDSIPNSVSTIHFGQNFNQDLNYIYFPNNLKYLYFGKNFDQKIRPKRIPHTLKHIGFDNLESNNTAKSLFFLHDGVDHLYLKNSQHYIYTPPSIKFVKIVNHHSTTRISNTIQCVTTKHKDLITFIQNPCLTKDKKTKQEKLNPSIHLDIFITENQFIHPNLFDFLIIKSICFDDVFDQLLLPGCLPNTLESIEFGSYNRPIKKEVFPKSLKRILFHKSFNQAIDCMLLPNSLTELHLGESFNQPIDKKSFPNSLTTLAFGKGFDQELSQDMLPNSLTKLSFGSFYHNNLLNWLPSSVTDLSVGNLEEPPTTLFPLRKLAITSVTKLWLGDSLIIESPNLIPSVIKEIRLCQCNIIEPIPHTLESLVLSKYIVCPLDSLINNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQU0</accession>
    <name>A0A8J4PQU0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S ribosomal protein L36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_006258</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL36 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000281">
        <property type="protein sequence ID" value="KAF2072423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQU0"/>
    <dbReference type="OrthoDB" id="25649at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1760:FF:000001">
        <property type="entry name" value="60S ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1760">
        <property type="entry name" value="60S ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000509">
        <property type="entry name" value="Ribosomal_eL36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038097">
        <property type="entry name" value="Ribosomal_eL36_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10114">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01158">
        <property type="entry name" value="Ribosomal_L36e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="80"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2072423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="11517" checksum="BC27CE3E5E995F56" modified="2022-05-25" version="1">MSTTTTKPIKRSGIATGINSGHPVTKRTLTSSFKKGLVTKRVAAQRDIVREVCGYAPYERRIQELLKSGLDKRAFKVAKKRLGSAQSGKKKRDEMANANRKANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR32</accession>
    <name>A0A8J4PR32_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_006077</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000264">
        <property type="protein sequence ID" value="KAF2072618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR32"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20507">
        <property type="entry name" value="CYCLIN_CCNB1-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20509">
        <property type="entry name" value="CYCLIN_CCNB1-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000001">
        <property type="entry name" value="G2/mitotic-specific cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048258">
        <property type="entry name" value="Cyclins_cyclin-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00292">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 4" id="KW-0195">Cyclin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="5">
        <location>
            <begin position="225"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="6">
        <location>
            <begin position="318"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="5">
        <location>
            <begin position="322"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2072618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="438" mass="49252" checksum="6581FB4F8238F6A2" modified="2022-05-25" version="1">MTTLNQQQLKPRAHSSMIMDENKIPAKVSLGMGASGVEPKKPMGLGLKTQRGALSDLTNNTVSGPVGIQKSNTANIKTMIMPQSSNSGIISRLVNRSKSAAVVEQSSVGANLRSSAMISGISHGGHLLKVAKHDISSNSVMEQQPVCIDDDMMMEDDGAMMVEAVQQEQPEDIDRYDAHDPQCVGEYVNDIFAYYRQKEIADRIEKDYLKNQHHINEKMRAILVDWLMAVHVRFKLLSETFFLSVNIVDRYLSKVPITINRLQLVGITASLLACKYEEIYAPQIDDFIQTSDNACTHEDVLNMERSILSKLQFHVSTCTPLHFLRRFSKAAGSDSRTHSLSKYLTELAMTEYNMVQYLPSMIAASAIYVSRRMTMKSGPYWNVTLEYYTCYKEADIMQCAQDLKEVRKRADNSSLRATKKKYTSSKLMEVSNIPVVEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRL3</accession>
    <name>A0A8J4PRL3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006290</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000284">
        <property type="protein sequence ID" value="KAF2072398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRL3"/>
    <dbReference type="OrthoDB" id="20328at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904294">
        <property type="term" value="P:positive regulation of ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21650:SF4">
        <property type="entry name" value="MEMBRALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21650">
        <property type="entry name" value="MEMBRALIN/KINETOCHORE PROTEIN NUF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="383"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="425"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="452"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="478"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="504"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="617"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="619"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="678" mass="78788" checksum="C0C9C4FC1D6E93C9" modified="2022-05-25" version="1">MEDTALMGYFHFIRYLHEITSPKIRFIFEIFCLVVISLFLSTLFVMHNQYVGKSDCIQDMFMRSVPKSRFDILTIEIKNEESFIYKDNIPFQPVMPKEEDIKKRVNHANHPLNHIKIQQDVVFRKQRTIAKNNDNSNENINNNNNNDILLTTTTTTNNNIKNKNDKDNISIIQKIFNLRLLSDPIHHIPLKILDSIFPNNKKQSKGNINTLLKKDIIDREQDDNDVNQQQQGKIDSSTMINHKEEEQQQQQQQQDQQQQDDTHYIDEFNDLVQYFLINTNPRYEFSYQKAFLLMTPELRTMYNISTHHIDIYTNETCLGNPFHKMILKILGYDIVMINNIVNSFMGKGYMKIISDNNIFNLANYSSPDRELSTEFIFHLIQKFLLTNLIFFSTTIIFGTSLRICEFCISRCFDHNPRQFLSSMAHNCLVGFIFILIQIGYISIFSVFLSTEYLVFLMSSLITSYFSTWGLRSKESIKYYPVLFLAVLYLLVFYIVTFPTGFHPIAFYSCYSIIEYLLILCLFNFEIPAVLENRIIRNNNNNNNNDTTNNINNQPIIGLNNNSNVVNSNNANANSNIISHQPIQLRSVQTEISNRSLHQHSSLGNLINSNSIPFIRNRADNNSNNHTNSDGESTTTTTINANNSNGSINYHHHTHHHISNDIVDNIANQLIFNDNIINN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS29</accession>
    <name>A0A8J4PS29_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007305</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000381">
        <property type="protein sequence ID" value="KAF2071389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS29"/>
    <dbReference type="OrthoDB" id="10250105at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004077">
        <property type="term" value="F:biotin--[biotin carboxyl-carrier protein] ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835">
        <property type="entry name" value="BIOTIN PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835:SF5">
        <property type="entry name" value="BIOTIN--PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="297" mass="33161" checksum="0609F35E769E5B64" modified="2022-05-25" version="1">MHHTLPRDNDRVYKWGQSTTNLGTRPYCFIYTHKHIDNWDQLFDPLKKPDIFQNQGEADASSRTDDPSIKIIVGDDSKKVGFDFQQYFQLLGTHLLGQNIIYGPVLTNDVMLCLDLLSMTQQGLTILADQQSDSTTVTGSNADQQFLCPLGSLVFSFKLQKVASNLVSHIDKLVGIAIVDSISNLSIVKSKKIDIKIKFIDNSSSHDIYGNNDKFGGILMDYTQYDDQYDVNLGSGINVLTDDTLGLNKIVGGSSSIILVSREEILAGFFNSFESMYFTFAQQGIEPFKDKLSKITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS52</accession>
    <name>A0A8J4PS52_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_006506</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May act as a GTPase-activating protein for Rab family protein(s).</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000303">
        <property type="protein sequence ID" value="KAF2072186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS52"/>
    <dbReference type="OrthoDB" id="27140at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000038">
        <property type="entry name" value="TBC1 domain family member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000011">
        <property type="entry name" value="TBC1 domain family member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF337">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="4">
        <location>
            <begin position="124"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="308"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="528"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="593"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="728"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="784"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="309"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="534"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="552"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="593"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="614"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="735"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="788"/>
            <end position="808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00043879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2072186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="808" mass="90376" checksum="E2CF995ACC687D2F" modified="2022-05-25" version="1">MDIFPQESSSSSSSNTTANTSVADSIFAPIGEITSTTATATTENTTATATMNKEQFTMSIMDASQEKVIESTLEHGNVGQEIHETKVNPVSRYKVEFNNLFCSAIPLQKPPFEILKMNALSGALRVSPLRGLAWKIFLGGLDINRIDHWKSDITKQRNEYQKLLQEHYINPRENSNNVVFDPLSQNDDSPWNKYFQNLEIQKTIDIDLDRTHPDNEFFQDKNIREKMLRILFIYAKTNGTISYRQGMHELLAPIIYLYEKEYSEYKKLDQDPNTMVDFIYDQKFVENDTYTIFYHLMKYTENWYAPGNTSSSTTNSPTTSSSNIPNTSHSSISSTPLISSTTSTSTSSTTATTTTASSTTTSPSLSTSPSQNNLLSTSLDGPSSAARDESKLNEVVLKCKYIHSTLLKQKDQELFHHLESLEIEPQIYLLRWIRLLFGREFHFEDVLNIWDSIFAYGESLLLIDYYCISMLVYIREQLLSSDSIHALKRIFKYPPVEDVSLLIKQAFVIKNSNFCSIASLVKNNPAKENSLLSKEQEKEKGREKESSSPTLKPNNSNNNNNNGKNKIDLFPETSELGLGQFIPFSTHLGSSLSSSKYSSSTSTPLIDPLSGKNSPLPTTPLAKPSSSSLSSSHTTPSTTTTTSTPLSTPIDSTSTTPNMSGININDISQKMPFRATSKHSTLPSKPTTPSIKDLEIQKLKDIQHYLGTSLGEILPIIEKSFKEISNNIVTSNNSDNDDNSSQGEDEKDTTTDDTNIENKKEFLLALAKIKQIKDMLLGELALPDPIPNFNDKNNNNNNNNSNNENKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSG2</accession>
    <name>A0A8J4PSG2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carbohydrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005697</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000234">
        <property type="protein sequence ID" value="KAF2072985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSG2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019028">
        <property type="entry name" value="CBM_49"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052879">
        <property type="entry name" value="Dd_Spore_Germination_Stalk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33239">
        <property type="entry name" value="CELLULOSE-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09478">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01063">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035313404" description="Carbohydrate binding domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate binding" evidence="2">
        <location>
            <begin position="42"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="141" mass="16077" checksum="2DD8C93467233AB6" modified="2022-05-25" version="1">MISPKLTLLALVFTIAIVNCHPDPQSFVNEPTPTPNYYDQFVKFNSSLTEKWEENNHTYGLYHIDVINIGDMDIVAMTFEGVQCHLRDLNTIWNMDVKKEETKFIFSLKPNTKIPIGGSFSFGYVASVENQIFNILSVNVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSN2</accession>
    <name>A0A8J4PSN2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006206</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small Tim family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000276">
        <property type="protein sequence ID" value="KAF2072474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSN2"/>
    <dbReference type="OrthoDB" id="1551503at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0811">Translocation</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Tim10-like" evidence="2">
        <location>
            <begin position="17"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="83" mass="9489" checksum="832FF4A80F2D2039" modified="2022-05-25" version="1">MPKPSKEEELIAYKLSKIQINGSMEAALNITNKCFQACITNFRVRKLDDNEQLCVYKCVDKNQLFLAALGNQFNKINEDFGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT48</accession>
    <name>A0A8J4PT48_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_004588</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000165">
        <property type="protein sequence ID" value="KAF2074103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT48"/>
    <dbReference type="OrthoDB" id="20689at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031941">
        <property type="term" value="C:filamentous actin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051371">
        <property type="term" value="F:muscle alpha-actinin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08368">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00821">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000009">
        <property type="entry name" value="Paxillin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050604">
        <property type="entry name" value="PDZ-LIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214:SF38">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PH" evidence="6">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="7">
        <location>
            <begin position="665"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="7">
        <location>
            <begin position="806"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="269"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="328"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="442"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="533"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="580"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="760"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="278"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="338"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="442"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="533"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="580"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="760"/>
            <end position="780"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2074103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="883" mass="99686" checksum="3E15E23BB25D32D0" modified="2022-05-25" version="1">MEYKSGALYKLGGGIFKIRKKYWFVLTTTHFQYYNSPEKTSNPIESISLNEIKNITLLQKEIGNLGTTYGFIVEYLNNSKLILQSDSLSSIADWVIKLDGLIHSDFKSFLNQVERRLLLKILYKNNIKGGIIKTSDSDQEWNYSSLGTLFIDAATNTGDNQEEKIDYHWDGEWLRSSSHNQTLGCGRFNGVYLAWYTRDLSIPDIRYFYDEKEKEYISDKKEFTFKWTRHFLASKYGSGEWIVEGHVPAPVVMFLQLIKYKRNSNIPGYPTPFPPTENPNNNINNQNIENNSSTPSLSSTPSSPSTLLSTTQTPRSTTISCEMMKPLNLSEDDPRHTSGSPISSSSPALIKLLKNSDDTSPTLASIQLPEGSNKASTMKISPFTKGNALLNVSNVLFTTPTYDEPEKSNDDSLISSFLTTSTSSDDLDLENKTIIDIQFNNNEDDNNIQNQNQSNNDQNQQNNNNNNNNNNSKIHSLEKLANIPKLNTSCLQLLDDTINQSNEIQTNRKSFSDPSLKLIEKVIDNEIKNEIKQEQEQEKEQEQEQQQQQEKHKDIEIIKEKVEKLKEQSEEQEESILNFSIDSTSSNQSSPPNINSPSFKNTTPLGSIIGPSAISTTPSMIATIEQQEPKSLLSSHRKPLAKSRSSDKVTTIINQISQSKQVRTSFCIACNLPISGHMAVAQGNHYHQECFKCYKCQKELGTKTFYRPIEQSSSSNNFVTLSSSPTSPSKYSSSPTLLSVSLSQSPVLSPSSLSSSISSISSSNTTNSNHSSNNNNSNSNNHHHHHHHHNKSPKYICQSCFNDTCPICPKCNSNVMYRCVNAMGKKWHPDHFCCVECQCLLLNTIYFEKNGLPYCQNDYNRLFNNVDKVVQQQQQLKDDRLYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTB9</accession>
    <name>A0A8J4PTB9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006512</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000304">
        <property type="protein sequence ID" value="KAF2072169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTB9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="569"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="612" mass="69464" checksum="EDA4BB5812D1CFAD" modified="2022-05-25" version="1">MWRNGDRILPLLDSKVLSERPMKISKVFNQDQVTMISSDGIYRFWNGNFSKSIIQSDSKFHYSGFIEADQNTMAVQFRDHELGIIYQNSNRVYKFQSPCKFKINRLFSDSCPIAEDLVCDFPKNKCFVSMNNGERNKIKVYDIRKRENLFDPSPKLITSRRYSGIIGKRVNGVIRKSLQTSHVYYCVQGFYPKYKSIEIHKINYDGITVKAIPYLGNEADSPQVCRNAIVNPLNNDIYVVTSNKLLKINDQDRIYEVRDIQYLNRDDIGLDTTEDSDFVNINGTQFFYVMMSNCNLQFYTTEPSCLSCNSPYWCYNGECKCPNDKYNSKCSNTPYLNNVTLVPGQVLNTKDLLLTGDFPIGETKVIVTPIIGKAFLTTILVANKTTIIASSKNNLTDIQTVTLIIDGVKTKFKYFSSEKSQNISSIEIHHNKIYINFSGPINITQSELKVTLSHVNLPIVSVSNSQIVASLRVGWFFGNRVLTISSPQFNSTIYLQQFLLQQFKKKLCFIINGLDKSNLGLEFMNAAINATETNSQCISSFGGCVTCNIQDPMSGIYYIKPTSKLQDLKDPDDDYEDGDGNHSGDQSNQSSLIVPNSNYFILLLVTLILLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTD1</accession>
    <name>A0A8J4PTD1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005184</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000200">
        <property type="protein sequence ID" value="KAF2073508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTD1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="2">
        <location>
            <begin position="79"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="806" mass="93718" checksum="3CC15104DFAB292E" modified="2022-05-25" version="1">MSNISTDIYCKCCAPTQIYQSPVLLSDCDHVVCRDTVVCLNDNCFICPLCNSNNDSICNNNDTVNQLNHKIHEYMIFNCKFHSSPYDSICLDCDERLCNICLQSHDNNHDIQPLVFLCELNESIKDKLQEKIKKFNFVIEKFQRNKYHELVEQDIQYYDQLKQSLSKEYDHLQEIMIEQVKQQISISKSKAIQEIDQYCGDRDSLKRLKQDIIEINSILHTYDKDSTKSSDTLFKVSDTLVQLREINDKTLIAKEKLNYRKEKLIHTKLSQLDIDKSRSDIQEIIAEKISLDSIANIKEQNPFLNREIYQRYEGNYLKNFVRNDSFICSFNCERDSGALLIPSYLSTLELEFKYIFIVDSSRKKQMKSILSTHFNMDFIEFTLEDTLENLFFKTIDCKDPVLLIYTGELDNIKTSNLNNKLKVINIYEDSYKVFYFKALEMDSCVQLFNDLLDSPFKGSDKQIHFIIKICNFNPNNLFKFCKYLNQGKVDINDLSNFRFKSKKLKMEKRNQAFINFDRYFKTISGYGKIRPVFSFLIKFHFSTPDISLEIVAKIDQKNVITPYSLLYCTPTSQNIHEYDENSLAQNLTLMESFIGPISITDKGLKLKLKNLEFDATLEFNVNNNLLAINRIDLSNSVETIIQSSLHLIPITGSLKVNNQEYLQTDKLILGRVCMSPIYFEDRGNISSWGMTQDNKPISLQLSLVPISFYRLNYLNFINYNNTIMDLGPFNINLNGNSVEIQSIYDKFNLIFTFKDQIIDNDKTGSFFGSLSGRVNFKDASDNIVQIEIKDFCAYLKISAPLHLIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTG2</accession>
    <name>A0A8J4PTG2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004349</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000152">
        <property type="protein sequence ID" value="KAF2074328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTG2"/>
    <dbReference type="OrthoDB" id="10463778at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="83" mass="9649" checksum="E3CB000E49B9AA74" modified="2022-05-25" version="1">MAFLLNYNFQLRNSNGGISAHPFFVNQRSPPKKYTLSHFDDSFPLDNQTIRPTIRDEDDFKYGGGFQKLDTVEDQLMFVIDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU83</accession>
    <name>A0A8J4PU83_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000198">
        <property type="protein sequence ID" value="KAF2073536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU83"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="831" mass="97148" checksum="6622D0355A68BDD5" modified="2022-05-25" version="1">MSLASQFSSERITRNDFFFHDTIDINDNNNLNTKTLKKNSNNDKEILFKKIFNNVVLRNHIFKYFKPPRGYYIYYNGVSYKDDDDMNLDDILKTKDQFFISDKLARYRNIIENSSSSNSSTHTYYKDWLDYPSKIDAKHNIFIQFDDKEKEYEIYRDLFQLFINTRRVGTFAKQIFFSDLLGNVGLKTVSKFIKLSIRDQYLEIKSPDGDYGFFSGCYDPKDRDDIDICLSLKGGDEMLLHSTLFGGNQEIDVQVVKQILLSSQKFNYDLRVKDIDQEVVVVENILDVNVIVREGKPFFISGSQIQDYLVCLIRDFFPCAVKFLTTGTLFTARYSTKTRVVLDIYEYDVAVEVVDQLIKENTRDNLSYIINVVSEDIRILMLFTNEKNESPNTSSILHIDFPDKMVASIDNLEFFIDNYPERLSPAKILENKYIKDIFTFTRILDEMTHGELVKDLIDEVYLMAVAGGNIEFMQYYLDCDPETLMASDIHQQDFFIDIILLGYLFDTQPGNPVYRYLASYYPPLFSKTKISPQRQRYYQESLFCLAIIKLDVGMAKAIYAPLDDTQKERIIKMFGMVTWPAIAHSKSFKEKKKGLLMAEFIKCEFAKFPFIKPYHLCWSLLYFTDQEFCLKMVLNYPPRLQASFLGSLVPGIVRTENSLFHLRHLIDNLAAPQLALLEGKIQKIVVEHLSCPDPPLKCSLYLFEKFQHILNQLSTIDSILTSAIKNNNHLLLHILLHRTHIRLIKPQSPQHEQQILNHVQHQMKSSKFLDSCTYFLFYPDFSHVEPHIDEKIYFLPQPTLSFDFDIRIDKHIDNIFSKNDLICKIIKNSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PV51</accession>
    <name>A0A8J4PV51_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_008486</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000527">
        <property type="protein sequence ID" value="KAF2070196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PV51"/>
    <dbReference type="OrthoDB" id="417037at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="266"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="359"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="7">
        <location>
            <begin position="94"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2070196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="381" mass="43745" checksum="043C435CB3CD31F2" modified="2022-05-25" version="1">MTTIKNNNSFHLLEKDSDDNDKSSLHNNNNDNDNHNDTLLVTKDTEKQDDDENENKYYKKEFILNLDHRQLLSYIWDQIRKAYENDTIKSAFAVVLYGTVSLCQTIFNKKVMTTFDYEGSNILLFLQMIVTIIILQLLNYFQILKVNTTLSYDIVKKLSPLSFCYIINVLLGLDSLKALNIPMYSALKRLVAVAILVMEYFILNKVSPPKVVASVIVMVFGAIIAGFTDLSFNSLAYTLVLTSCLFQASYLIFVKKIARNMPTYDMLYYNSVLSLPIIIVLMLYRDEVTYFRSYQYLDNPYFQLYFTLSVFLGFFLNFCIFFCTSVNSPLTTSVTGQVKNIASTIIGAMVFKDIIIQPFNIFGLVVNIIGSIWYSFLKLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW82</accession>
    <name>A0A8J4PW82_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GDP-fucose pyrophosphorylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008002</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000460">
        <property type="protein sequence ID" value="KAF2070676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW82"/>
    <dbReference type="OrthoDB" id="10062280at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016772">
        <property type="term" value="F:transferase activity, transferring phosphorus-containing groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042350">
        <property type="term" value="P:GDP-L-fucose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012887">
        <property type="entry name" value="GDP_fucose_pyrophosphorylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15045">
        <property type="entry name" value="FUCOSE-1-PHOSPHATE GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15045:SF1">
        <property type="entry name" value="FUCOSE-1-PHOSPHATE GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07959">
        <property type="entry name" value="Fucose_pyrophosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GDP-fucose pyrophosphorylase" evidence="3">
        <location>
            <begin position="110"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="573" mass="65902" checksum="7D62BA650EDD24B3" modified="2022-05-25" version="1">MIELGFEDNIDILNQYKSIHKRKTNDYKLFLNDNVDNRRKDQFWDTIVITAIDDQQKDYYELVLREKIKVNEIPSFIEYIVISDPKGYKIGCGGSTIYVLSILKEKLGAKFDKSKILLLHAGGYSKRLPNHSMTGKIFASIPYTLVDDGPTCSMLEMKLIMLIDIPKKMKPGVFLACSDDIEIFDSWDIEFKVDNGFIAIAQPGTLDIGTGHGVFVLEELNNFKQNSLNLCKKFIHKPTKEKMALENAILSNGLVLMDSCYYFDHNISSLLLQYYSENNPIKVEIDAYSDFLQPIGKDAKPDYFHSKNNVTTFTPEIAIEREKLFKLLNDNQCKLYMIPYIPSTFIHIGTCHEYIEHFTNNFNKLGFKRILYSLVNNNNDDSGLLKEVTILHSIIKSKNSSIQSNSVIEYCKFTNINLSIGSRCILSDLENDDNNHNNNNEQCLEINIPSNTFIQTQCVSDKYVTIVFGIDDNLKSTTKPLTIFNQPIENLFKTNPIQFDSETHSLWNASIFPICDSPSKSLFASLNLFFNHVKSNQQQHQLYSIERCLMEKNLAKQYQRRLNLTKEIKNNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWP4</accession>
    <name>A0A8J4PWP4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FG-GAP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_004457</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000157">
        <property type="protein sequence ID" value="KAF2074247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWP4"/>
    <dbReference type="OrthoDB" id="270568at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34284">
        <property type="entry name" value="FG-GAP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34284:SF1">
        <property type="entry name" value="FG-GAP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035191722" description="FG-GAP repeat-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="678"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2074247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="725" mass="82274" checksum="4AA580C4374650EF" modified="2022-05-25" version="1">MRKRDSGLLVISIVVILLSLYNQNDFKANNGFTIPIDTNNYENSHYPQFFEQLPQPIITDIDGDGKNEIIYVTNDYKIKILNSIDNFNLEQTPNSPRHEASLKTKVGVSIGRRPIALKTGYTKPFSSGDYNRQVIVVVTDGWAILCFDYKLNLLWESFATDEVESGHYHSEISITVVPVSLMGQDEGLVVVGGRLEPLPNSIHKPHYKIPLAPNGESNKNDEDKIDFLSEGDDDYDKVNQKTVHKEEDSELHKEEESHFSFYAFDAKTGLKRWSHEEEDFKPVNPHEKEGHRVNQHSYKQHIYNELDHLGEVEWKVYRDSILSSLPHTWSSNFDTRLQARHFEKKVTSIKGKSDNTDQQQQQQNTGGWKSEFVGLTPTQDIFTLLTYQQQDDLIVKDPNVIVVHNKNGIEVVQIHSGRTVCRLVMDSTDFHRNSDHFIVYLDVNQDGVLDQIHSIAGNVPALNNFKDTDKKKKNSGSCVALAMAGVPPRDYLFMRSICGFSSGLDIDPIHINTGNHKDPHYFQTAPPTFIQNPITKKLDTIFFVNTGKISSVQANGKQNWKVTTESTWKRHIEPHTFPSIQVISMVSNSNYYTQQQQQQKQQYILAIGGESMTVLDDQGNIVIEKSFEEKAEKNPVMGPPVIGDLNNDGYNDFLIPTLFGYKVYLTEKTSYTGSSSSLLLPLIGFIVLVSLLFLLITTNQSKSSSFNSNDLRSKFNLNTKKRSTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWZ0</accession>
    <name>A0A8J4PWZ0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007627</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000418">
        <property type="protein sequence ID" value="KAF2071051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWZ0"/>
    <dbReference type="OrthoDB" id="10290201at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="503" mass="57884" checksum="ECA5E7FA69EBEF91" modified="2022-05-25" version="1">MKSFLSIFRNCYLRSLIRNHVFKNTIINIPTLEYLDDNHQHLSVFTKDDKLNHNIYIKYSLKINQDPVELDQFSNSIHKYLVNEIQFARGKKEETLDLGLVHDQVQRLTLYLGECVKSVNGKLPDSIVELNIDEHYKFADGVVCPALDSILSDLPKNLRVLRLADEFNLSSKFQLPDSIIDFQYSSTRANLERLVISNPNKLLESAWLIVTSNEDLHWVQDKYWINKLYVLKNVTIKIPPHVRFIEFNTDAILENLPLELEKLRVGKLASTQNQEPVSKILKQYLPYLKHLELGQLEEKLEQSTYSDCLESLYIHFYDKDIDVGVLPSNLKTLHLNSFNQQLKIWSLPNSITDLALMSYNQPLRPSTMPTQLKKLNLSKFNNTIEANSFPDSLTSLQLDGYQGSFEHVGKLNNLNNLKVDVLNQSIVDILANTITIGFNTIAENTSFARTSITDLCLWDTTWGDVKTLPDNFLPPNLVYLELVNFKINSDKIPLGCKVVHKNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX52</accession>
    <name>A0A8J4PX52_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SREBP regulating gene protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_003098</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the SPRING family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000093">
        <property type="protein sequence ID" value="KAF2075593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX52"/>
    <dbReference type="OrthoDB" id="70142at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000640">
        <property type="term" value="P:positive regulation of SREBP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019352">
        <property type="entry name" value="SPRING1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13481">
        <property type="entry name" value="SREBP REGULATING GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13481:SF0">
        <property type="entry name" value="SREBP REGULATING GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10218">
        <property type="entry name" value="SPRING1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="61"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="65"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2075593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="267" mass="30931" checksum="AD0B4771E5C39A23" modified="2022-05-25" version="1">MRIPLKYGIFFVILIGIGFTFIIFYNTTHQQIKESEKDIIQRKKLDAILKSVNFRWGPSYDTDNKQSLSSSGTATTDAEQQQQQQLPPQQLRCKNTVQGIDYVTDSQGYLCSRQYIDLVTGCCNQTILQQHSESFQSGSSSQDDKNNNNNHNNNKLPIKQKDTDSIYLKYQDPKEKIFERFTCTRCKESMGCCKEYEFCVSCCMRSDKIPTLRDTFFSILEKNYSSFIKSQFDLCSVACRTSSNSLHNQREYKQQEYKFCYGPSTPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXB8</accession>
    <name>A0A8J4PXB8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_004231</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000146">
        <property type="protein sequence ID" value="KAF2074449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXB8"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00876">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000080">
        <property type="entry name" value="Ras-like GTPase Ras1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2074449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="214" mass="24452" checksum="2F0399AFED96429C" modified="2022-05-25" version="1">MSGNNRLNNNIHKICILGDGGCGKTSLIVQYTSNHFVEYYDPTIEDSYRKQLVVDEQACLLDILDTAGQEELSCMMSQWIRSCSGFVVVYSITSRSSFDQVNIFRDQISRVLDRENIPMILVGNKCDQEELRQVTTQEGQDLANCLGMLHIETSAKTRLNIEDVFYNLVRKMRESEKSFSDKSGDKDKKPTFKSMVHKMNKIKKSSLQNVCKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXB9</accession>
    <name>A0A8J4PXB9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002955</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000088">
        <property type="protein sequence ID" value="KAF2075713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXB9"/>
    <dbReference type="OrthoDB" id="17470at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="99"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="36"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="279" mass="31910" checksum="29A5ECE0911B6CA7" modified="2022-05-25" version="1">MEPLGYNNSSFLLEDNERLQKENWEYKKAGCANKDHIALSTQLQQIKQEIEIIKKENTNNIDELNYTIAQLLEQLDKSNKTIDDLKKSNKEMLKKLKSRIIDKKTFKCLNDWIDSTKRLHFELLYSASDNEFTTSSFHKACDNKGATITVIQTTNGEVFGGYNSTSWSSDGGFGGDSKCFIFTLVSKQGDRVVCQPSKYFYGDLKSQYVYNGEDNGPTFGLKDIVIRDGIDKSYKMFPSSYRDPQHGTTSNTTTPSPYFILKDYEVFKIVYNSKIKVIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXC5</accession>
    <name>A0A8J4PXC5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Threonylcarbamoyl-AMP synthase</fullName>
            <ecNumber evidence="3">2.7.7.87</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">L-threonylcarbamoyladenylate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_004209</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonine + hydrogencarbonate + ATP = L-threonylcarbamoyladenylate + diphosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:36407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="EC" id="2.7.7.87"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SUA5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.87" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000145">
        <property type="protein sequence ID" value="KAF2074464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXC5"/>
    <dbReference type="OrthoDB" id="412787at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061710">
        <property type="term" value="F:L-threonylcarbamoyladenylate synthase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.870.10:FF:000009">
        <property type="entry name" value="Threonylcarbamoyl-AMP synthase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.10">
        <property type="entry name" value="DHBP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11030">
        <property type="entry name" value="Threonylcarbamoyl-AMP synthase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="Sua5-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038385">
        <property type="entry name" value="Sua5/YwlC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005145">
        <property type="entry name" value="Sua5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050156">
        <property type="entry name" value="TC-AMP_synthase_SUA5"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00057">
        <property type="entry name" value="L-threonylcarbamoyladenylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490">
        <property type="entry name" value="SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490:SF16">
        <property type="entry name" value="THREONYLCARBAMOYL-AMP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03481">
        <property type="entry name" value="Sua5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="YrdC/RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="YrdC-like" evidence="14">
        <location>
            <begin position="83"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="335"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2074464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="462" mass="50504" checksum="1188EAEC34F4317F" modified="2022-05-25" version="1">MKSVIHNWCCGSDNINKIKSLFYSSLIISSYSNSNSKSNSNNSSYIRFYSKQINTTTTINKTTIYNHKMASFNPLAKIVEPTLDNLVEAGKKIKNGGLVSFPTETVYGLGANALDKDAVLSIFEAKGRPLTDPVIVHIIDSEYAKSLLELTDKQTEIFNLLVGSFWPGPLTIVAKSSSLVPMIVTAQTGYVGVRYPKHDLAQALIAHSGVPIAAPSANRFGHVSPTSAMHVFDDLGHSPITILDTTTAYQAPCKIGIESTVLKIVSDTDLVILRKGGVSERSLRQLFVNRTDINISSINKTVSNTTNEGQEAPGQLLTHYAPDIQTYILNTCSSSSSSNNSSSSSSKSLDSSSSNNNSNNNSISLKECVYIDFGGELDFKLKSQCLAYRDLSEKSDIEEAANQLFSVLRWSESVENAKIILLPDFRKQSLSHLDSDAIFDRIFRAASGKLAQINFDTNEIHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXD3</accession>
    <name>A0A8J4PXD3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_007428</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000396">
        <property type="protein sequence ID" value="KAF2071246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXD3"/>
    <dbReference type="OrthoDB" id="15794at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026669">
        <property type="entry name" value="Arsenite_MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002935">
        <property type="entry name" value="SAM_O-MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43675">
        <property type="entry name" value="ARSENITE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43675:SF1">
        <property type="entry name" value="RIKEN CDNA 2700097O09 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01596">
        <property type="entry name" value="Methyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2071246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="340" mass="39277" checksum="4E23D8D0F67A4691" modified="2022-05-25" version="1">MSEPTNKSNKRKKQEEIVEQDETTAPIDLELIKKQFKDLVRPIKDNDDRLDFISFVCSHLNIQIDPIPDHKPKESKQENVLATIIQDIKDSLPDITTEAPNEFICIPKNNSFKGYTKENTINVDGFLYTDEDIDEMVESGKLQSHYCLDCNSKSVATLNYISHSTTPNQLKYMFSEAFLGDTTDKKLLDIGSRLGSVLYTGYLFGQCSKLTGIEINQFFVDLQKNIIKKYKMTDKIDIVHGDIMKNSDLVQQSDIIYMNNVLEFFQENKDLHIEFWKFIQKHTKKGMKLVTIPSLEESFKNNKIPLKLKGWVKKIDTDIPVPEDDPTFGDYQEIHLYTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXI3</accession>
    <name>A0A8J4PXI3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pmp22 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_001932</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000050">
        <property type="protein sequence ID" value="KAF2076743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXI3"/>
    <dbReference type="OrthoDB" id="10267969at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF112">
        <property type="entry name" value="PXMP2_4 FAMILY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2076743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="21701" checksum="8F2FB85D63557D68" modified="2022-05-25" version="1">MSNSKPANLKECFTNWYMKQLKAKPIQTKALTSATLSFASSVLAQKLIERKKIDWSSVIKFTIWGLISSPLSHFWHMILERTFKNVKDKYQVWGKLLADQLIFAPFINIAFYTVLSILDNRAFKPKSILIKLYFDLFPTLKASWKVWPLAQFVNFKFVPAQLRVLFGNFVGFCWGMYLAILSTKKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXQ0</accession>
    <name>A0A8J4PXQ0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_004670</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000169">
        <property type="protein sequence ID" value="KAF2074019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXQ0"/>
    <dbReference type="OrthoDB" id="282152at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF12">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="1 5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="4 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 6 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035322143" description="DNA-directed RNA polymerase subunit" evidence="9">
        <location>
            <begin position="20"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS-type" evidence="10">
        <location>
            <begin position="70"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="6">
        <location>
            <begin position="4"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="4"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2074019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="116" mass="13302" checksum="426DF28349B9D4E8" modified="2022-05-25" version="1">MLFCPLCANMLLLESASDGRVNNQSQFYCSTCAYVFRINNKVTTKVPLLRKQVDSDVFGGDEAWLQAEQCEAECGKCHHKRANFMEIQVDTIDEPKTSYFKCIKCSFLWSKKKDTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXS2</accession>
    <name>A0A8J4PXS2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003382</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000105">
        <property type="protein sequence ID" value="KAF2075305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXS2"/>
    <dbReference type="OrthoDB" id="24489at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550:SF10">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140860">
        <property type="entry name" value="Pseudo ankyrin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2075305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="663" mass="77672" checksum="2BA5F86988696A42" modified="2022-05-25" version="1">MNITEILFFKVYRNRYIRTVIFDKLSKGYGYYDVVYADWCVRSKNYSLLKEKLKNSDFLTLAENSSCDIAAIPDHELFVECFTRFKNYFPKDLYIITERAVFYNQLSNLKWLISKGYTLLDSEVSFINAIRHSNVKMLKYLCNDLHLEKCATRSLVKALSQENIEIIKIVLQLCLDEIKGEQESSIILDFIKTGNLQVFEMAKQVWGDSSIERVIREKLLYVYESAVGNYDFFNHIFKNYGTGWDGEFYEFDKIDSIVQHIVVPAFCGGHFDVLSLLFYNNCLPIDLFSPPATTKEFDRNQDNCFEFLNSFRKENRLHLKLIGLSNIKDALSNIENVEFYNSLGFPIDSTCLLKCLDHFDKDVFLYLWNNGRFKISKDKIFDGVRDFGNLLVYKCFETNNSWVLEFLHERKFSLTLPSSWFQHFNYIDGNYHFLLLLLKIAPFAFKNSLFFTYSIKKSINMGSIKLFNFFNQLLDSFNLDMKKEIISSCLSTITENGSMYLIESILGQGDKDDASIGENAAACNLEIVRSLLENGYIFTEKAFQVAVTKGSIYTVEYLYKFIPGGITIKEIHLYEAFKRKRDKIKCLQFLFDNFKGQIPELFYTTDNIQILDLVTKNREKIIFFKNMIDRVKENAIRNNIFDVLKYYSLPYKKVDCGWDCEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PY26</accession>
    <name>A0A8J4PY26_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GPI mannosyltransferase 2</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_002908</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the PIGV family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="AJWJ01000086">
        <property type="protein sequence ID" value="KAF2075775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PY26"/>
    <dbReference type="OrthoDB" id="10252502at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031501">
        <property type="term" value="C:mannosyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004376">
        <property type="term" value="F:GPI mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007315">
        <property type="entry name" value="PIG-V/Gpi18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12468">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12468:SF2">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04188">
        <property type="entry name" value="Mannosyl_trans2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4 11" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="348"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="436"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="506"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="533"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="572"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2075775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="596" mass="69157" checksum="45FFD15E0D6B3AD8" modified="2022-05-25" version="1">MDYQHHQHHHNSNNKIINSNDENNKIISLYRSQRAIIIKVAVIVKLSIWIYALIVSQFLNNFDSSTTLKSVTPYSNNSTNYTATASLDFALQKEYLTNHNNESVLVDTLIKRVFVKWDSLYFIRIAEHNYEYEQNHAFFPMFPFLMNMGAKCIQMIPGIQLSFSDSIIVAGFLISNVSFVLGAVQLLKLGYIIFHIPKIAFISALLYCINPAGVFTTAVYTENTFNLFIFTGLIYTYGGDFHWQSNSRSLHMHSDEISTVRKIITTTIGALFFSLATCTRSNGILMPGFIVFRFYSSYFIEIFKHLFKFAGWILRVKNTTPSPPADQSTTTTNHQTRSITNLLGWKELILYPIVIVLQVTIVLVPYIAFQFYGYSRYCVGGQDIAPDAHKNGVWPRPWCNTEATGALFPNLYSFVQNHYWNQGFFNYYTVQQIPNFLFATPIVVISVFAVYRYLCYFIQNGNQMKYLYKLPLNNTLNTADTYNSNAYMLFPTGQNSTQLIYFYTPYLLPFVLYHLFLTLFSVSFMHVQVITRFFMHSPILFWFCSMFFLKYSNKTPPPNPDQRESSNKTKKYLILAYFLFYNILGTVLFTNFYPWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYM6</accession>
    <name>A0A8J4PYM6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Chromatin modification-related protein EAF3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_002542</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000071">
        <property type="protein sequence ID" value="KAF2076135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYM6"/>
    <dbReference type="OrthoDB" id="124855at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.30">
        <property type="entry name" value="MRG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008676">
        <property type="entry name" value="MRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038217">
        <property type="entry name" value="MRG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026541">
        <property type="entry name" value="MRG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053820">
        <property type="entry name" value="MSL3_chromo-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10880">
        <property type="entry name" value="MORTALITY FACTOR 4-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10880:SF15">
        <property type="entry name" value="MSL COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05712">
        <property type="entry name" value="MRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22732">
        <property type="entry name" value="MSL3_chromo-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038133">
        <property type="entry name" value="HAT_Nua4_EAF3/MRG15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51640">
        <property type="entry name" value="MRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MSL3 chromodomain-like" evidence="8">
        <location>
            <begin position="8"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="MRG" evidence="7">
        <location>
            <begin position="196"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="88"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="130"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2076135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="371" mass="42638" checksum="CA28193FE262443B" modified="2022-05-25" version="1">MDDTLQIYEENEKVLVHFQSRLYEAKILKFEVRPESKLKDSKKKGFYFIHYLGWKEKWNEWVESSKILKLTEKNKELQKKINAKAISAAAANKKSNNKGSKKKQKQKEIEDTDNSDSVSESEESSDDDNNSTGDESNHSSSSSFSSSTSKNSNNNSINSNNSSVGSNGSNSNNNNNNNNNNNNNINNNNNNNIQNNKRKRDVNPKTFITIDIPVALKAKLTDDWSLINNDKCLIPLPRKPTVSDILNEIEKEHSTLEESKQIENFNELLNGIRDYFNKALGAILLYKFERYQLFENQKVYKIPADLYGAEHLLRLFVKLPELFDISHLESKSKTLIKSTFDEILRYLENNIETLFLQEYTPATAQYIKNSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZB6</accession>
    <name>A0A8J4PZB6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ribonucleoside-triphosphate reductase (thioredoxin)</fullName>
            <ecNumber evidence="3">1.17.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_000712</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + [thioredoxin]-disulfide + H2O = a ribonucleoside 5'-triphosphate + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:12701"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="EC" id="1.17.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class II ribonucleoside-triphosphate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.2" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000014">
        <property type="protein sequence ID" value="KAF2077988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZB6"/>
    <dbReference type="OrthoDB" id="14890at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008998">
        <property type="term" value="F:ribonucleoside-triphosphate reductase (thioredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.70.20">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050862">
        <property type="entry name" value="RdRp_reductase_class-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054158">
        <property type="entry name" value="RNR-II_ins_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040763">
        <property type="entry name" value="RNR_alpha_hel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43371:SF1">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43371">
        <property type="entry name" value="VITAMIN B12-DEPENDENT RIBONUCLEOTIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21995">
        <property type="entry name" value="RNR-II_ins_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17975">
        <property type="entry name" value="RNR_Alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51998">
        <property type="entry name" value="PFL-like glycyl radical enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="9" id="KW-0170">Cobalt</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribonucleotide reductase alpha-helical" evidence="11">
        <location>
            <begin position="47"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="B12-dependent ribonucleotide reductase insertion" evidence="12">
        <location>
            <begin position="210"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2077988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="716" mass="81571" checksum="BD699C0E1898B70D" modified="2022-05-25" version="1">MINRFIHKYYNHQQTIGSLILRNVVSYHQKEKTLDQSNTTTFKPFSLSREFINKYKEKKAPFGFDILGELVYKRTYSRIKENGVNEEWYETVERVVNGTFNMQVNWFVKKGIKFDYNSLASKAEKMYEKIFTMKFLPPGRGLWAMGSSITEEKGLFAALNNCAFVSTRNLIRYPSKPFTFLMEASMLGVGVGFDTLGADGKFLVLGPMENKEPLLFTIPDSREGWVESVKLLLDSWLLRGKVPVEFDYSLIRPEGSPIKGFGGYSSGPESLKTLHQSLDEVLRKRKGQPVTTTTIVDIMNLIGRCVVSGNTRQTAEIAFGDPNSEEYINLKNYRLNPERQSYGWTSNNSVFANIGMDYTKVCERIVENGEPGFAWLSNMKNYSRMNAMEYDKKDFKAAGGNPCLEQTLESFELCCLVETFPNNHSSIQEYCETLEYAYLYAKTVTLGKTQWEKTNEIIERNRRIGCSMSGIAQFINSNSLDTLKKWCEIGYKHLNRIDRQLSLEFQVPYSIKKTSIKPSGTVSLLAGATPGMHYPISEYYIRRVRLHKDSYLLKGLIQAGYKVEIAHENPVNMVVEIPIHAGHKIKSVNQITMWEQLSLAAFLQARWADNQVSCTVSFDVEKEGPQLPVALDYFQYQLKGVSFLPRSSDGAAAYKQMPYEEITQHQYETMKSNLKPVNFNSSIGEEPTPDKYCDSHNCTIGTSSVVESELLNPNNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZN1</accession>
    <name>A0A8J4PZN1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_003333</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000103">
        <property type="protein sequence ID" value="KAF2075357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZN1"/>
    <dbReference type="OrthoDB" id="439710at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00777">
        <property type="entry name" value="AspRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04317">
        <property type="entry name" value="EcAspRS_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="entry name" value="GAD-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00044">
        <property type="entry name" value="Asp_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004524">
        <property type="entry name" value="Asp-tRNA-ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047089">
        <property type="entry name" value="Asp-tRNA-ligase_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047090">
        <property type="entry name" value="AspRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029351">
        <property type="entry name" value="GAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00459">
        <property type="entry name" value="aspS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001750">
        <property type="entry name" value="PRK00476.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF5">
        <property type="entry name" value="ASPARTATE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02938">
        <property type="entry name" value="GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55261">
        <property type="entry name" value="GAD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="7">
        <location>
            <begin position="236"/>
            <end position="676"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2075357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="706" mass="80543" checksum="A1346D0A278FC9C3" modified="2022-05-25" version="1">MNVLRLNQKNNKLLLLNGGGRFTLSHQKKNKNFLTPNTHNQHQSILSSPTFNNNSKQQYTTISNNNDKLKQKNFNVIERTNTCGNIGSKDIGQDVILYGWINSIRNMADKVFLVIRDGHGKVQVYFNKSGDQQIKLKSNDPDIDFDESTPIDESIKQLKMESIVCIKGKVIARPLSMVNNKMSTGEIEIDAKEIHLLNNCVDLPFTIEHDGEKVTEEVRLKYRYVDLRRDEAQYNIRLRSKVAMAARNFLVENQFVEVETPTLFRPTPEGAREYLVPTRFKGQFYSLPQSPQQYKQLLMVGGIDRYFQLARCYRDEDLRSDRQPEFTQIDMELSFVNTEMIYSIIEGLVKKMWKEAGHNIDYKFPFYTYDQVLSKYGIDKPDTRYGLELNDVTSIFNTADTQVGIFKKELEKPSNKFKESIPMIKCIKLPQVMSKFTSKELDSIVTDSKTLLPNSPGVLMVKVSNKDTEWKSSISKYLSENEKQQLISQLDVNDGDVLLFSAGPRCLVEPLLGKVRIHCANMMKEKGLLEINPKKFNFLWVVDFPLFTPADYMDENSQLQSTHHPFTSPHPDDHHLLLNPSSGPSDYLKIRGLHYDIVVNGIELGGGSIRIHNPEVQLKVLKNVMRLEDHMIQRFNHLLTALSMGCPPHGGIALGFDRLCSLLVGSNSIRDVIAFPKTSGGRELMTNSPSAVTSKELEELHLSLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q012</accession>
    <name>A0A8J4Q012_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Swiss Army Knife RNA repair protein HAD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001517</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000036">
        <property type="protein sequence ID" value="KAF2077196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q012"/>
    <dbReference type="OrthoDB" id="5596992at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990259">
        <property type="term" value="F:histone H2AQ104 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008649">
        <property type="term" value="F:rRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018812">
        <property type="entry name" value="SAK_HAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335:SF23">
        <property type="entry name" value="OB FOLD-CONTAINING PROTEIN, NUCLEIC ACID BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335">
        <property type="entry name" value="RRNA 2-O-METHYLTRANSFERASE FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10307">
        <property type="entry name" value="HAD_SAK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Swiss Army Knife RNA repair protein HAD" evidence="1">
        <location>
            <begin position="15"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="519" mass="58957" checksum="2A2FE2ED5F6AC16F" modified="2022-05-25" version="1">MNLYIYDFDGTLFKSPEPNPSLWHTSLLGKINGMPQENGLGWFQETMTLDEPYVPSNPSREEWFNENVLSDALKHIQDENNIVCLLTGRTELYRDIINRILKSVNLNFHHVGLKPINIPNVARESTMDFKKKFILNLLNEEYKNKIKTVTIYEDRPKHVEAFTKFLEGLKQLSKFHIEYIQDGSKYLAHDLETDLINYLIEKNGQGMIRKKFVDYTCSRLDKESQKKLLDMFSLPKEWLIKTHHVTMNQGVYNPKQWLAQDGVEQDLEKQFALLSTTTTSTTSTSTVSNTAESTTITTSSSVVTATATTTTLSSLDSLKEEYPIGKWLDLQVEAVGISANAVAVKVKGCPTCNIIPHCTIAIHPLAKAVDSNKIRKWFSVDQFEKGKTITLSSHDEEELKAYKEMMDKPQQSPKSPSQQLPPVSITFTRPLDSQGLKIRGVIEEIGKVGIEIDKKAVQAAKAATGDPNKWNIFPMLQKLYPKLGNKELQTETINVKQWVKTNKVEDIKVLQDYLSKLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1U9</accession>
    <name>A0A8J4Q1U9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_001503</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000036">
        <property type="protein sequence ID" value="KAF2077182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1U9"/>
    <dbReference type="OrthoDB" id="5344169at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23513">
        <property type="entry name" value="INTEGRAL MEMBRANE EFFLUX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23513:SF6">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="424"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="562"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="602"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="461"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="502"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="462"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="502"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="516"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2077182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="631" mass="70063" checksum="542149D77796BC99" modified="2022-05-25" version="1">MDSQSSPLENSHQNNNVGQRFDSVQENTGDLKDIEITSQHQHDVEKGVENNFNSPPFDQDGPEAKKYSRFHPHFYTQDHLNETKIIIASMSFKFAFETFSSALGLLVLTRFDDYGVTLLAVMNIVYFISQSIGSVLVGPFVRSFRASRVTSFVLFFMAILISILIIVEACTGGTLDKLGSWNKWLITPVYLLMGLFLGMVEVSRKLTPRQVLGNNNAKLSKLNGLIHLFYEASGTAGAFLSTLLISKLGPIYALIHQPIFFLIGSILFFSVSQPFPPSLVSDSQEINVSSFTLFKNRFKNPIVRAAIKGSHEVYLYFHALFVGARHILSREYWWIITTYVLPQVLHRLLENLLFPTFAKKVLNDGSLSGILTGGSNMGEAVGSLMVVKFGRYVKNPLWWVRADGLFCNLLWILVYPPKLSSPSAIAWSMVPIWVVVSSSWAAGDISLLSFIQSQFPLNVASQDHHKKEEEQDKSETKNEQPAANIDLENPDRQISDVEMSTIGSSTNSSTESLQSIHHSTTDAPRVAPSNQSDTSVEDLTLDDTGKQVDISDEDDPPPGSPLASVIGFLFALYAIIISLLSFGLGRTMDHFINQGDTQRGFFYIAGVGFTVCGGLVILSSFFAMHKELRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1W2</accession>
    <name>A0A8J4Q1W2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pre-rRNA-processing protein RIX1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_000295</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RIX1/PELP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000005">
        <property type="protein sequence ID" value="KAF2078428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1W2"/>
    <dbReference type="OrthoDB" id="20900at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012583">
        <property type="entry name" value="RIX1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34105">
        <property type="entry name" value="PROLINE-, GLUTAMIC ACID- AND LEUCINE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34105:SF1">
        <property type="entry name" value="PROLINE-, GLUTAMIC ACID- AND LEUCINE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08167">
        <property type="entry name" value="RIX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pre-rRNA-processing protein RIX1 N-terminal" evidence="5">
        <location>
            <begin position="108"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="775"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="42"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="775"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="791"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="806"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="843"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="876"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="903"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="939"/>
            <end position="959"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2078428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="959" mass="107545" checksum="742385DC051D5D3E" modified="2022-05-25" version="1">MSTKITQPKAGGNVGNLKSKSTPTSPTPASSSKKRNSEPDITQPNKSTNKQKQNTNKKMKKQTTTTTNNHQQHHSLSHDNILKCVIDTYIMPLVGDQDKIPHFLDIQSYLPNIIVLLHQNQCLKLTLNSTEPSSEKLKTKWVEILNFMLKSNNIDSVWMGVHLLCETIRVCDYDMMVQKSEQWITLLCNLAEQQKKQLNSQTKRDKNDVLISFKEYGSITLAVSLLVEKASHWADLKRSLTTNSAMSPIVSIILQSLESHLGYPTQSKIDSLVAISSLITSVGTAIKPYLNKIETQCYPLLFSLDKSIQEAASSVISNLPNCVGINSTDLYWDVTVQKILVELNRIIDQIYNGLEDHDDLDSDGKPVKLPTRSINLPNSNKLINLGDDQIVNMLKNLSNVKFPEPPKDTHQQSSFILRGFHGLCLCLMKILSSPTIAPSPVPLDEIIKLLCRILNGNINHLNFSHLLMSYSLSQVIYSLSQLHLQSFILLSQCLKVFKKSLLVHCKTISSLLLRPLRSLNYSLMSPIIHSQIYTTVTDAIEAFGPSCCESLVIPLIPILLRDIQPYIPEEISATTTTTTAAGSNTTANGTTAGSSLAKSKSQKDLIMVQDFTSITIKQNGLKTLSCILLYCGSYLLSSPNIGSGFEQQQQQQQQQQQSNLRLEIDQLIITLLLQCQSLSSNKKNSSTYLNSSFHCWRYRLRLYECLINSVLKPVPYVPPVLPYAIRILTVGMNTDYYPKVRNLCSKGVSLCESLIHPQSPSLVSNTNIYSRSFNFSSNNNNNNNNSNNNNTSLYQSNEYTNSHFESEDEHDDDEDVQDESESEEGQDEIDQETNFNQIKDSDQESDENGDDGDDDKLDISFNSIDNDEQEDDEKPTSTTKSKSMKSNLFDFGTISKTNQKNDQDEEEEDEELDEQEGKEEEVNDKSTKTNIATKNNQDGENDDDDDDDLPDIVDDPPSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q216</accession>
    <name>A0A8J4Q216_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_000364</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRIAP1/MDM35 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000006">
        <property type="protein sequence ID" value="KAF2078381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q216"/>
    <dbReference type="OrthoDB" id="19091at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990050">
        <property type="term" value="F:phosphatidic acid transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007918">
        <property type="entry name" value="MDM35_apoptosis"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46403">
        <property type="entry name" value="TP53-REGULATED INHIBITOR OF APOPTOSIS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46403:SF1">
        <property type="entry name" value="TP53-REGULATED INHIBITOR OF APOPTOSIS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05254">
        <property type="entry name" value="UPF0203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2078381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="87" mass="10314" checksum="F9D4A89428874879" modified="2022-05-25" version="1">MGSDKKEDTKKPHPMTDPNLCKDLKERHDACFNKWYTNAFLKGTSTTSECQDEWEEYQVCLKDKLKQWNLEYLMDKNKVADKHPTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q217</accession>
    <name>A0A8J4Q217_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PRELI/MSF1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001412</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000033">
        <property type="protein sequence ID" value="KAF2077287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q217"/>
    <dbReference type="OrthoDB" id="407630at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006797">
        <property type="entry name" value="PRELI/MSF1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037365">
        <property type="entry name" value="Slowmo/Ups"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11158">
        <property type="entry name" value="MSF1/PX19 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04707">
        <property type="entry name" value="PRELI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50904">
        <property type="entry name" value="PRELI_MSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRELI/MSF1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="284" mass="32484" checksum="D1157F860DED10D2" modified="2022-05-25" version="1">MPLFQTILHTYEHLWADASLAHWKKYPSPERPDVLSVDLLSKELDPETGILKITRLIVCKGNTPTWLKSILGSSECFFYEETTVDPKNKVMVLKSQNLNFTNILGVEEVCTYTPDPNNKDWTLFKQEAKVTSSIFGVARKMEAFCLDRFVANAGKGRKIMEDAILKVKLEAEESLAQIETTLDKTITNIKIEAENLKDEACEGIQSILPITKLDDESTTTLNHSTRPKYHIVPHQKLKYIQDQEQRALEFNIQKIFKGLERSQENTALFDFDYSLFSEKASEIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q299</accession>
    <name>A0A8J4Q299_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000123</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000002">
        <property type="protein sequence ID" value="KAF2078623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q299"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="178" mass="21045" checksum="943927E7D5DDE54B" modified="2022-05-25" version="1">MKFFSRLRSKKETKTTETAGTTETKIETAETAKTTETKTCCCDVDARPTRHLNEIFFSVWRNKFLKTHIRRQRFQGVEIRKKISYLKKNVESFRNLLLDCEMNSYVTIEISNSEDIDQYKQFKDRDIITKIIWLHCQPIPPNLLTDNVKMLTLYNIKDFGCYSIPNTVDKILKQTFLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2H4</accession>
    <name>A0A8J4Q2H4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000631</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000012">
        <property type="protein sequence ID" value="KAF2078080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2H4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2078080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="66" mass="6814" checksum="D43EB83C87F8EE41" modified="2022-05-25" version="1">MSILKSIASINFGVAQSKSSNFFNSNNNLVGAGKNQNCQGLVGQVVSTVDGTLMPVAQTVDDILGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2R0</accession>
    <name>A0A8J4Q2R0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA2/NAM7 helicase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_005602</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000228">
        <property type="protein sequence ID" value="KAF2073076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050534">
        <property type="entry name" value="Coronavir_polyprotein_1ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788:SF8">
        <property type="entry name" value="DNA-BINDING PROTEIN SMUBP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13245">
        <property type="entry name" value="AAA_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="6">
        <location>
            <begin position="1498"/>
            <end position="1712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="44"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="180"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="238"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2073076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1821" mass="207187" checksum="E56DB0E4888FEF20" modified="2022-05-25" version="1">MEDKSTPPNSTDNIDNDNQIVVIEEKLLKIESDIDTILNKTIENYCNNNDNSDSTGTDNSNSDVKLEVLPPPPIESESNDVLEKDSIDLPPPPVVLPEESVDKSNIVEPSASPSHDTNNSNNSTQSMEDVQSEEPKQQQQEEQDKPSTLDGFKESLKKEIFSFLGSAMKDLLTTTILSSQSSSSNLENDQNEKVQETEQVEDLNKPVSSDKDLDSLIDTFDLLGVGKETAKVDQEPLSTTIDSNSPISTTTTTTTTTNSNSTTVIPTTPIPQIKLKKTLKNFYEQGLIELSDKCFFVFKEIKCEVRMVLEGSTPYFVLDMPVANDKVVVIKEQSATTLIKKYLKEYNNLNEQAIAIPTALEGNKLLFIKDTLMDDYKFKQQDTTATPIPSADDLLKSPGIQTPYKPLPKASTASSSIQINSNVSYAFRNKSSKTTPSPSPIKIEYEVEKAKVRPSGVGRYFHFGCDRYLSFSTRSVSSTSVPVNPMGSIQQALVDDGFRWEEKVLEEMEARIAAEKSDIKIFKSTSPDSNDIPVNETLRILREEKGTFYIIQSTLDPPPTFKRNLAKSIGFSMSKPDYLLITTDSNGKREIEILDAKSTNKITFSQKIQLAFYYLLLNDIIAYHQIPDISVSYNAGIYLKGNYKPEPFSLKDPVSILSTFLFGDRTSSVSQLEKILSTPKQDSSWIVSERCDGCEYLEHCTKQAVAEKSLNSVSYLNQAIRNIVPPKSELGTVSEIEYLLALHDKVNEDNNNLKEIEPSLLSLYTTELIRALPKLKAVWNDSVEITNNFDVLLTSSVDLQVFINVVHEPCSQSPYQWGLMVIESDNKYLFNGQQFSDLVIKLADIYLDCDQKKSLQSFIFDAFQREYFLSNCFKLLKHSTLDSNQELQDKVLVVLRTLLNNNDWINFNIDYYPEIHLPRKAPVKTRQEDNSLILVQDLVKKIYALNINPIYTLEKIVDKLPCFKQGPQSNNENNTVTTTLPLNGNQMYEQFIKKQLDASVPINYQLECQRMIVNDLRAQVFNGENSKPASLFVIRPSSSFFKNQHMKHLYFCYQNETISSCRTIRNKRSNSLLLNQFEGVYSVLKLIEKVQKTPKTDLLTFVADIPKQNFPKFTNFFTKDITSFYITAVENEKHLSKINDNQFTMIKDKFIHSLSLVKESVSIIKENNLSYTISFQGKTPSCDLLLEKHFIFFEMFDIKYFQMHSTLMASYEEMDNDKDNAFLNILDNPLPWITDLKDQDKFLQSIKGVVESLKDASKDSVNKLTMTKSQMEIFDSFVKKRLQLVRGPPGTGKTHFLALLVLIFMEAYHRLEKPLRIAITSFTHTAINNLLQRISSLMKEYKTIIGQDPNFYLRKNQSTSKKDTPEFADDPMIEILDKKKDFNHKYLVVGSTSWGLATMKQEFDLLIVDEASQLSSFIGATVFNILSDNPLNGTEGGQDSFYSRVIVCGDQQQLGPVLNDQYSSKKDLVIKKDKLNPKLHKSIFSCLKSLFNQHIAKMYPDPSCTNRPVALLSLYENFRMNQELCQFSSSTLYGPLYQCFNPSQNTIKSLDITNHGSPITHSIFNSDRSIHTILLNDNYFGNKEVEAQIVKDIYEFLRIEYSNANNNSDQDEIENNFWNNSNVLGVITPLHEQRNLIIPSILSLQEKKWKLKNLVTPIVNTVEVTQGKEYDNVILCYNGFNLNNKISEFSFNLNRLNVGFTRAKKRFILIVSTQLLYPDEIIFNSHRMSKAYQHLFSYIQSSQIHEFDITKIQLNNPTITTDNSHSVDINNESNEINPKEEKDIIMDIKNEAITTIDTTIPTNDNTSNQNLLPQFENLSFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2R2</accession>
    <name>A0A8J4Q2R2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit L</fullName>
            <shortName evidence="4">eIF3l</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_001037</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eIF-3 subunit L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000023">
        <property type="protein sequence ID" value="KAF2077650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2R2"/>
    <dbReference type="OrthoDB" id="15082at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03011">
        <property type="entry name" value="eIF3l"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019382">
        <property type="entry name" value="eIF3l"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13242">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13242:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10255">
        <property type="entry name" value="Paf67"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="PCI" evidence="7">
        <location>
            <begin position="358"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2077650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="578" mass="66784" checksum="2C62DC93791D45A2" modified="2022-05-25" version="1">MQNNNNNRKGGNKQQQGGNNNQQRQGGNKNANYNKQQGGQQGGNQQGGNQQKGRKIPTNVESFPITLTPAQVQQHQQYQQSMQNNQSGQFQSSYLPPAQQLFTPVINYFVNFFTNFKSQSHSDILVSYDNFFKSCETYYSNCALPTIDVFLAVLGDKYEDTFTDRFFVLLYREIYYRYYFALVSQPNIGICVESWRNYSAIFDTLLEAATPNEVEIDLPNAWLWDIVDEFVYQYHTFAKFKLKKVDVDQLTEIWDTTSVIRYLYLIIKKSQIFSANSSRFENATDDFCSHPVYKMLGYYSVISLVRVQCLLGDYTLALKTLELIDMGKKAMYTYVTACHITLYYYLGFAYLMSRKYNEATKALNTILVSVASKTKSQNFQSDHNEKKIDKMYALLAICQALYPNKIDENVNNTLKEKFGDKLLKLQKGDISVYEELYSYSSPRCINAVNPHLSGHSSNALDAHRLQMALFLEEVKQQSLLTTIRSYITLYSTISIQKLAGLLQIDPEELRIKLTCYKHKNVNVLGKGETKYHNQVDIDFYIDQDMIHIDNRLYAKETDSFINSILKFEGSLTPQYNWM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q355</accession>
    <name>A0A8J4Q355_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">allantoinase</fullName>
            <ecNumber evidence="6">3.5.2.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_000713</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-allantoin + H2O = allantoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17029"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15678"/>
            <dbReference type="ChEBI" id="CHEBI:17536"/>
            <dbReference type="EC" id="3.5.2.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nitrogen metabolism; (S)-allantoin degradation; allantoate from (S)-allantoin: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the metallo-dependent hydrolases superfamily. Allantoinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.5" evidence="6"/>
    <dbReference type="EMBL" id="AJWJ01000014">
        <property type="protein sequence ID" value="KAF2077989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q355"/>
    <dbReference type="OrthoDB" id="16559at2759"/>
    <dbReference type="UniPathway" id="UPA00395">
        <property type="reaction ID" value="UER00653"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004038">
        <property type="term" value="F:allantoinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000256">
        <property type="term" value="P:allantoin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006145">
        <property type="term" value="P:purine nucleobase catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000122">
        <property type="entry name" value="Probable allantoinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3330.10">
        <property type="entry name" value="Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017593">
        <property type="entry name" value="Allantoinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050138">
        <property type="entry name" value="DHOase/Allantoinase_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018020">
        <property type="entry name" value="OHCU_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036778">
        <property type="entry name" value="OHCU_decarboxylase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03178">
        <property type="entry name" value="allantoinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43668">
        <property type="entry name" value="ALLANTOINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43668:SF2">
        <property type="entry name" value="ALLANTOINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09349">
        <property type="entry name" value="OHCU_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158694">
        <property type="entry name" value="UraD-Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00482">
        <property type="entry name" value="DIHYDROOROTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="11">
        <location>
            <begin position="61"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase" evidence="12">
        <location>
            <begin position="486"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF09349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2077989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="650" mass="72596" checksum="7AF25CEDB5F0E445" modified="2022-05-25" version="1">MIYSEPTKVIRGTKVVIDGKIQPATLIIKDGVLVDIKDYDFELPADWVVLADYDENSEMVIMGGLVDSHVHVNEPGRTEWEGFVTATSAAAAGGVTTIIDMPLNSAPVTTSYENLLAKIESMQGKLRVDVGLLGGIIPNNAHEINRMVRDGGVVGFKSFLVHSGIDEFPHVGEADIQMAMTYMSDLKEDGVVMMFHAEIGEPIDEACERLKQENADPKEYNTFLQSRPRESENIAIDRIIDLSKKNNVNTHIVHLSSSDAIQALHQAAHDGVPITAETTFHYLFFESENVPYGNTLYKCCPPVRESENKDLLWKALEKGDINIVVSDHSPCTVNLKLLDEGDFMKAWGGICSLQLGLSIVWTEAKKRDIPLTKLSEWMCDAPAKLVNINDQKGSIKIGRDADFVIWNPNHKYTVSQETMMVKNKFSPYNRFELYGRVHQTILRGNLIYTDGDQSVDQILGKRVVPTRIHTRPTYPEPYLPNIQLLNSLDKKSFSDCLSLLFEPAPPLADRLYSSRPFTSFKDLVDQATSIIAKLAADEQLEVINAHPRIGVNANDIKQISSLSFKEQGCDKDGADISAVDEIYAALDELNQKYESKYGFKFVTFVNGRSKSDIIPIFKERLESSTKEKELSLGLSEMMLIANSRLNKLCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3B9</accession>
    <name>A0A8J4Q3B9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">FZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_000716</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000014">
        <property type="protein sequence ID" value="KAF2077992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3B9"/>
    <dbReference type="OrthoDB" id="3241054at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035303233" description="FZ domain-containing protein" evidence="8">
        <location>
            <begin position="22"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2077992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="289" mass="31114" checksum="651182A8E2D9CBE5" modified="2022-05-25" version="1">MKSFVLLSLMIICSIFTATHGHAVLRTPQPWNTQASKANPCGGGNPNTTPRISYCPGTKATVVWEVQVGDGTGPVTFKLSTTHDVTKFDTALTSTGATPNAVGTYSFQVDIPNTSCQDGLCYIQAYSDSNWFSCASINITPDCKATELALVPIEIEDLPYCNMVNKRTVLLPPGITNKDQFVARDATALSTFKQYMNNSAVIGTPSATCGNLLTEFICDQSFPLAPGSDGAQVTQVCAETCTEFKEVCQVVSHDALYPCANYPKCSDAFKQLPSLFILFFIVIVSVLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3L1</accession>
    <name>A0A8J4Q3L1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_000420</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000008">
        <property type="protein sequence ID" value="KAF2078229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3L1"/>
    <dbReference type="OrthoDB" id="17323at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF12">
        <property type="entry name" value="EUKARYOTIC INITIATION FACTOR 4B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="76"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="161"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="242"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="242"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2078229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="326" mass="33821" checksum="940B3A0F38705F93" modified="2022-05-25" version="1">MAKAIESKKRKKEESSESETESSEEKVNTSSDSESEDEIKVIDGPSNGKSSFAKSSNDDSESNADKTQQYAAEDELKVFVGQLPSRASQDDIAEFFKDCGEIMKINVLSSNPQRIAAFVTFATEEGKNKALTYNGADFNGNGPLKINPANSKPSNGEGEPNTIVARNLSFTVDETLVKQFFEGCGTITRVNLPVYEDSGRLKGFAFVSFEDVASVDAAIALSGENFEGRQVQVERSVKGGGFKRGGFGGGRGGRGGFGGDRGGRGGFGGGRGGGRGGFGGGGRGGFGGGDRGGRGGFGGRGGGRGGRGGFGGGDRGGFGNKRFRSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q449</accession>
    <name>A0A8J4Q449_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MD-2-related lipid-recognition domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_005073</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the intermembrane transfer of phosphatidylglycerol and phosphatidylinositol.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NPC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000193">
        <property type="protein sequence ID" value="KAF2073616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q449"/>
    <dbReference type="OrthoDB" id="6409159at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015918">
        <property type="term" value="P:sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.220.10">
        <property type="entry name" value="Ganglioside GM2 activator"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036846">
        <property type="entry name" value="GM2-AP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003172">
        <property type="entry name" value="ML_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039670">
        <property type="entry name" value="NPC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11306">
        <property type="entry name" value="NIEMANN PICK TYPE C2 PROTEIN NPC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11306:SF0">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROL_PHOSPHATIDYLINOSITOL TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02221">
        <property type="entry name" value="E1_DerP2_DerF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00737">
        <property type="entry name" value="ML"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035288686" description="MD-2-related lipid-recognition domain-containing protein" evidence="7">
        <location>
            <begin position="21"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="MD-2-related lipid-recognition" evidence="8">
        <location>
            <begin position="26"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2073616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="142" mass="15438" checksum="445E59AE3BACD2A0" modified="2022-05-25" version="1">MKLLSLLGLVLLMTLFVVHGEELSAWTNCGTKNDLFTISSITVTPDIPTRGSAVNVTLVGQLHETVPSIMVKLQIKYNNKIVMEKDRDVCSKVKCPVAPGQYHHPIQTTLPSLAPAGKYTVHIFAISGEKTLACANIPLTIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q483</accession>
    <name>A0A8J4Q483_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005011</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000190">
        <property type="protein sequence ID" value="KAF2073661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q483"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="325" mass="38330" checksum="A114DF0B839802B6" modified="2022-05-25" version="1">MKREIDNDISTDIDDDGDVVVGIKLFKSSCQYLSTEPQLPLLYKSLTLIHSQRQEYLESLDNAQRKRKLSKFVQNSISKSNSGNSNHRYNEQERKERLQKLRQCVVTLDEIKFYQSIPTPMKKNQIVYKNTESEYSDSFANINTLNYEYKDIFLSQPILDQIDLIENINSNSNNDNDDSKGEQEIKNSDNETKEKEEKESQQVEEEENQKEQEQEQKQENLDVDGGDDQEEQLDESVNLFPYYPTPIKIYNIIDDNPENKIIQSKLFNKNYITKKYLYRNSHFYNIYKDYQNPNAITNTVNDTTTPTPTLSKKQLKKKKFFKSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4A2</accession>
    <name>A0A8J4Q4A2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MACPF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005008</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000189">
        <property type="protein sequence ID" value="KAF2073691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="21110at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00457">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MACPF" evidence="2">
        <location>
            <begin position="1051"/>
            <end position="1392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1290"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1295"/>
            <end position="1305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1795" mass="201824" checksum="6E01B68D4E614003" modified="2022-05-25" version="1">MIVVTLGIKDQGTGLYEINCVSPNGIFRAVDVIISGSLFEGVFELVFDLSKNFLIKDKLSLELIDFNNNRKEISYFTFDLHEATHILNPKEITIADFSYFKFNHESIDTTFGHRKTSLFFRVSDLPIDFTPQIYINESSVEFVEPKRHAYSFIGKYHRGMEMFVIDIELPSKLFDTNLSYILFAPTKIERSALETKFGSDATIKITSKYFDNYPPMITNFKFIKSHVVEIDVDQEIEIGWEFEITDNPNGFCYGTFEITSDKDGSPFVFNISSIDRISGNEFQGYYPITFKVYGNTIDQSYSISSVNLYDKNGRVSTYPTWDYPTRLSPLLRLSKDHSIKLVCKNVVSLNTLAPSLKPVQGFSYSFNPVDRMVSFTIKVVDNGGRGFSPFSKHNPIVYVDGIYDHIKVQTNVCGSCANTASLITYIAKVELNYNLIISGCSFSVFGIVDRHMNFYGANAKSLKELGFKSTLKKTDIYFIPSISSITPISDIGGDITIHGERFTSKCKVYTQNNGGEFTERTVKILSSTMIITTIPSSKTDINVKVLYSPSKYTNTIKVSPFISSKKKFVYIDTNSNCATNCGTIDNPYKTIKEALTKSDSFSDTLLILPGVYVGEENTQIDITVFREIFSLSGPETTIINCEGYGYGIKAKNAKRLILTGVTLRNCVSDQGGALSLYNTPTRLVNVHFVNNTALNGGAVYVFGQELSIKDSVFYNNTVVSNGASIYADLAIVKIKGDLTSFVPNIDLSSNTDSSGRAKDILCRNSTIKIDETIDMNKADFECFDGCDSSYANKPLCLSTIVNYKAGKSVCTKESCLSRPDECSCLFSGLVLETYSQDCSVLEIDQCESYHQQPAHDIVLEKHSGGQNKIVNRFYGYLSVSESKYVTLLFTGSNYGYILKINTKEQFSMIQNTRFNQTKTIYLTHKHVHFIEIIVFSSVSQSSQRYFQMTPFLSINDDIFYSNLICGDKVLDKMESFANDDDDRISNPFYCPADLEYPTLGSNPYCGDKICNEVPDECFQDCFDVLTKTCPTRTVPDGHIAPGFFVSDDTLGELIYNQFIWKLPGSDHLTFGIDIVNGEEASAPIFQFDYCQHIAPNIMEDIYRGNVYLLPDQFNARPLPECTYSSSTKSNDTSFEVSQSLQASASQSYEASVSGGFMGTSGSVTAAYSQEKSSKEASKLSTNSKEKIFKTDLYCKTSFVEMDMEKIALHPNLLRSLSEVVDVEGVVELVKKHGTHFYNSAFLGGKLTQLTIVKSSDVKKSSESSFSQSAAASLSASVNSPSFSVSASVSGSLDSSNDKSQEESNRDSSTTSRILTYGGAPAAFSPAQDGESSPTYSDWSRTIDLLPVPIDYRLVPIRNLIKDTWKSKHNPDTNLKSLWEQAEDIYYAMNQFDFSSSQSGYSLIFELDSSRANYETNNSPELEIIYWVKGKDKPLVFRQKMELVHVGLDGREYGYLYKNSKLYSHARCFRYTAFNRDISTSYATDFMSCDYGAQANMQYPIRLDFTGPNFFSSVKTPEIKLTFKEGDESLINYNNPAKIISWLNNQAILFNQDGVITKGTDYYLSTAFENRWAWHTSKSYLFRNYYPGSHTVSCTDDFSYTSSGTCKDSIIFKFGKSIMAGNKEIPDNNNPVQGEWFATLGEERPLINWFNYKAPIGNDFKGTLEIAHRSDIGLQSRIYWVKVFYPSNDNNWLFDIKAYHIKSDQNSIHSKEYLEVNIEPGTPKDRFSPYKFITLKPLEISEPKQLFFYHNYNYPVDGVLSTGFESIYNLPSYAFILLDGKNITFLDSGTGNSYHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q9X1</accession>
    <name>A0A8J4Q9X1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptide-methionine (R)-S-oxide reductase</fullName>
            <ecNumber evidence="5">1.8.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_001012</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(R)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:24164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12314"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the MsrB Met sulfoxide reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.12" evidence="5"/>
    <dbReference type="EMBL" id="AJWJ01000022">
        <property type="protein sequence ID" value="KAF2077696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q9X1"/>
    <dbReference type="OrthoDB" id="44061at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033743">
        <property type="term" value="F:peptide-methionine (R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.20">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028427">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002579">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00357">
        <property type="entry name" value="peptide-methionine (R)-S-oxide reductase MsrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46081">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46081:SF8">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01641">
        <property type="entry name" value="SelR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51790">
        <property type="entry name" value="MSRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MsrB" evidence="6">
        <location>
            <begin position="60"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2077696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="183" mass="20836" checksum="C0876DCF12612DED" modified="2022-05-25" version="1">MFGVISNSLKTISNRLYTFSLFNHTKIICHSTRYTLPTATTTNSYRYFCSNNNKKMNLSDDEWRVKLTPEQFKVLRQKGTERGGTGEYDKHFEKDGVYHCAGCDAPLYKSDSKFDSGCGWPAFFDSVPGALTIHEDNSFGMKRIEICCKNCGGHLGHVFKNEGFKTPTNERHCVNSICLKFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4QA23</accession>
    <name>A0A8J4QA23_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000907</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000019">
        <property type="protein sequence ID" value="KAF2077786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4QA23"/>
    <dbReference type="OrthoDB" id="13613at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34003">
        <property type="entry name" value="BLL2395 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34003:SF2">
        <property type="entry name" value="SNOAL-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12680">
        <property type="entry name" value="SnoaL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="1">
        <location>
            <begin position="21"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="113" mass="12942" checksum="C36C161E8DBB9349" modified="2022-05-25" version="1">MSNIRSNLEAVLKGIQENKIVETFDKYYHDDVVMFEKGDSTNRVGKAQNRVAEQGFASNATIHELKVLKVLVDGDNSAYEAEMTFTYAGNKITKTQWSIQQWKDGLIIKEEFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4QBD2</accession>
    <name>A0A8J4QBD2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000211</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000004">
        <property type="protein sequence ID" value="KAF2078461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4QBD2"/>
    <dbReference type="OrthoDB" id="29321at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF180">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="506" mass="58110" checksum="9B235D4FCD317601" modified="2022-05-25" version="1">MNDTFFAIFRNSFIKKLIRNKIFQDSLLDVDTERLKENHRFLSLFSEQDKLENNIYIGLLVDSRQKLIQYYNDPYKYLINKINILDIKGIEIDFNHFRNIVVLEFFIGHDSVLVGEIPDSITDLKFKSNEIYGGHYCDWILSKLPRNLKKLSIPERWKMVEKTTCDKTYILPETLSDLCYIGTYNDYNFVFPKNGHAFKSFQINIHSLEALKWVENNTCVSKIFIVNLPDCPAVTCDLIPSHVKDLCIWDKIPIHGIPDTIDSFHYRDFLDDNLKLPSHLKSLDFLGFASRETPLLEKDVFPSSLKDLSIWCYNSPLAKGVLPCNLSILKLEAFNQQLCQGVLPSTLTSLSMSDFNQPLEPFVLPPRLTKLYAPSFKNTLSKNSLPSSLTILDIDEFSGSFEHVSPLDHLTRLCVGTLNASVKNILLNVVKIKIHLDIIGPDLDLHDNTSIQELGIECHQSVQGIRAECLPANLQIFYTIALEIRGGTYPYPFKLNPQRNKTKQKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UNT6</accession>
    <name>A0A8J4UNT6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Actin-related protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010304</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001028">
        <property type="protein sequence ID" value="KAF2068371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UNT6"/>
    <dbReference type="OrthoDB" id="7340501at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10211">
        <property type="entry name" value="ASKHA_NBD_Arp5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="384"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="384"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="685" mass="80344" checksum="B4BCDF722B1CE192" modified="2022-05-25" version="1">MTIFKYKEYESPPEDQVYTDYQSKHLTSPIIIDNGSYQLIFRSLVGKAKSTSNPIVGNSLKESDLSRLSIKSPFDNNILVHPPSQESIFDYIFYKLGIDKNIENPIVLTEPPSNPSYCRKYTSELLFECYNVPSVVYGIDSLFSFYNNRELFKNNGKEALIIGSGHNTTHVYNVQNYKVQHYQTKRINVGGATGTDYLKKTLQLKYPNHKSYLSSLNYINQLKEQHCCFTKESFTEQLTHYQSSGPEQQQQQSDIIQLPFEEIDYEKLEEERQRKIQQRKEFGIKLKEMAEIKRKEKKQVQEDRLALLNSILEVKKSNNVQEFESLLRKESIGNERELVEEMDELNFKLFGTKKETVAKTEKEEFPLLFIADTELNADQLKEKRKQKQLKNLKDGKKAHKRKRDEEKEREEAQSKYEEECFIKDPEQYIKDLYQRRKKVLDKRDARIKLKTSKQQMVRRESRLRTITPTERLDREEELDVEEEEENRCLNQFEKLLNKFDPGWNSSNNIDLDDSNINNNNNITNSNSSNNNNNNNNYETKEDYQIVFGVERIKIPEILYQPKAIIGLDQMGLIETISSTILSQLPLDVRSLVSQNVFLTGGNVNTKYFKERIEYEIRQLNEPGTLLNIITSKQGNSSLDAWYGARKWTLDNMNQWSNVSISRQDYQEKGYDYITKHFASNLSIFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPJ0</accession>
    <name>A0A8J4UPJ0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rhodopsin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_009362</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell projection</location>
            <location evidence="7">Rhabdomere membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDIP1/LITAF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000689">
        <property type="protein sequence ID" value="KAF2069316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UPJ0"/>
    <dbReference type="OrthoDB" id="18688at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037519">
        <property type="entry name" value="LITAF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006031">
        <property type="entry name" value="XYPPX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292:SF6">
        <property type="entry name" value="FI16602P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02162">
        <property type="entry name" value="XYPPX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00239">
        <property type="entry name" value="RHODOPSNTAIL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00714">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="LITAF" evidence="10">
        <location>
            <begin position="101"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="13"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="28"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2069316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="193" mass="20871" checksum="B21AFC3880C30EB6" modified="2022-05-25" version="1">MTNLDKVDSSNGTPPPQAATAPVPAPGPDAYGQYPPQGYPPQQGYPPQGYPPQQGYPPQGYPPQQGYPPQGYPPQQGYPPQGYYPPPQGSPQQTHMAPQPMPTVVVAQAPIMVPRFLDVPVDITCGHCKAAIVTKTEYCVGSMVWLICALLIICGCWLGCCLIPFGIDSLKDVEHSCPNCKQKIHRFDRLSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPP6</accession>
    <name>A0A8J4UPP6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Right handed beta helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_009169</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell outer membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000648">
        <property type="protein sequence ID" value="KAF2069511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UPP6"/>
    <dbReference type="OrthoDB" id="75921at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003368">
        <property type="entry name" value="POMP_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02415">
        <property type="entry name" value="Chlam_PMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035226361" description="Right handed beta helix domain-containing protein" evidence="10">
        <location>
            <begin position="24"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="504"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="145"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="146"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2069511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="552" mass="61474" checksum="29D9C907770649BB" modified="2022-05-25" version="1">MNKSIIVLFCWLILISSCSVVLSEITTKSDLGTKIYISNDNGLDGDGCGIDVAKQCKTIQYAINIFKNTHTIKRYNRRSDEDDEYVYSYPPLEISMDCGLYDSLGNSDINLFELDITLSSQQQQQQCDVLINNYNTNNTIFTVIKDPSHSQRDSASRFQNENNKNKDHTSQTLIHFKNLKSTYAKSNNHFGSFLYANTFHKELIIRIDIDQCEFNSSVPSHTTTIPESSLIAISGHYDAEAVEPRTFISIRKSKFSNHQGSNYKQLSSAGVLSIRSKANDVDLSVDSSVFSANTGGVISISSCHSFQLANSLISNNSQNNSAVHISNCKRFAIANTVFQSNRAKIGGALSITKSRGDIDSTQFKENTASSLGGAIHLSGQTHLSMRNTLFDGNIASQGGAIMSESRSSLEISQTNFTNNRAIVFGPSLYCSYSTVDIQGHDCIFQDNYHLGKYPKQIYQGVDCMNVLHEEPTCTFKGTNQTTLDEILSNGNCSRGWTNFFFDHFMTIICVSMVLIFALAFFTLASMSEKNLQAHRDHINQNQKTKPMDKKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPR3</accession>
    <name>A0A8J4UPR3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YbaK/aminoacyl-tRNA synthetase-associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009112</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000638">
        <property type="protein sequence ID" value="KAF2069571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UPR3"/>
    <dbReference type="OrthoDB" id="1058301at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04332">
        <property type="entry name" value="YbaK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.960.10">
        <property type="entry name" value="YbaK/aminoacyl-tRNA synthetase-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036754">
        <property type="entry name" value="YbaK/aa-tRNA-synt-asso_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007214">
        <property type="entry name" value="YbaK/aa-tRNA-synth-assoc-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30411">
        <property type="entry name" value="CYTOPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30411:SF4">
        <property type="entry name" value="YBAK_AMINOACYL-TRNA SYNTHETASE-ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04073">
        <property type="entry name" value="tRNA_edit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55826">
        <property type="entry name" value="YbaK/ProRS associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YbaK/aminoacyl-tRNA synthetase-associated" evidence="2">
        <location>
            <begin position="78"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="4"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="234" mass="26825" checksum="0262E772A186F464" modified="2022-05-25" version="1">MSLNDNLITKQNQILSKLEELQKRLDNITASDVVTENEKRISNYLANDLKLDFKLTRVPSTYYDWTLEQRSNYLKTPSPEYLCKTIILENTECVNSDTSIQSNSRYYAVIIQYTTKIQSHKIFKFIKNINPQESSKKFHFRLAPPEVTQQMTGFEFNAVCPVGGNTRIPIIISKHIMDLPHGKAWLGGGEVDLKLEIDTLQFKQRVEESGTLVYVTDIIDPVNITTNSTTDDLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4USD8</accession>
    <name>A0A8J4USD8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">FACT complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_005177</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the FACT complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the peptidase M24 family. SPT16 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000200">
        <property type="protein sequence ID" value="KAF2073501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4USD8"/>
    <dbReference type="OrthoDB" id="10251642at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035101">
        <property type="term" value="C:FACT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000017">
        <property type="entry name" value="FACT complex subunit SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.350.10:FF:000006">
        <property type="entry name" value="FACT complex subunit SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000005">
        <property type="entry name" value="FACT complex subunit spt16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.210">
        <property type="entry name" value="FACT complex subunit Spt16p/Cdc68p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029148">
        <property type="entry name" value="FACT-SPT16_Nlobe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056595">
        <property type="entry name" value="Fact-SPT16_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013953">
        <property type="entry name" value="FACT_SPT16_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013719">
        <property type="entry name" value="RTT106/SPT16-like_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040258">
        <property type="entry name" value="Spt16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13980">
        <property type="entry name" value="CDC68 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13980:SF15">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14826">
        <property type="entry name" value="FACT-Spt16_Nlob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24824">
        <property type="entry name" value="PH_SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08512">
        <property type="entry name" value="Rttp106-like_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08644">
        <property type="entry name" value="SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01285">
        <property type="entry name" value="FACT-Spt16_Nlob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01287">
        <property type="entry name" value="Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01286">
        <property type="entry name" value="SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="FACT complex subunit SPT16 N-terminal lobe" evidence="13">
        <location>
            <begin position="24"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="FACT complex subunit SPT16 middle" evidence="14">
        <location>
            <begin position="549"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="domain" description="Histone chaperone RTT106/FACT complex subunit SPT16-like middle" evidence="15">
        <location>
            <begin position="813"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="931"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="467"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="934"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="968"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="984"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1010"/>
            <end position="1055"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2073501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1055" mass="119797" checksum="B4BD28B2D9B2721D" modified="2022-05-25" version="1">MSSANGNGSSASSNGGSAPKAPKLDLPSVSKRLNIIYDSWDSDSELYKSADALLLALGLPDEDRPYQKVTAMQSWLFGYELRDTIIVFLKKDIFVISGTKKIQLLEDLSNVEIKKHKIKCHFLPISKTDNKESFNKVFEEIKKAGSNLGVISKEKYFGDFAKSWETALNESGINKVDITPGFSSCLLVKDAQEIKYMQTSAKISDKMLVGQLLPKIEKTIDNEEKVSHNDLAEFTLGVFGKPEKLKGKLGANVDLSSIDYAYTPIIQSGGNYDLKPSANSDDEKLKFDTIIVSLGARYKDYCSNIARTYFIDPVDEQKKNYHFLLELQTLLLKSFKAGNKVSQVYDKAVEHINNVKPELASHFLKNCGYGIGIEFQESLLVINANNNRVMRSGMAFNFVLGFQNLLVKAGTDKEKKYSMLVTDTILVGDDGAEILTSESPKEATEVFYEIAEDDDDHSVTLEMPEDVKDVTGKVRETKEKAKSVEEKRRQHQQILAQRNKQEQEHKIRQLEGLAKNKSAEVDYTSITNKLPDIYSAISSYPTETVKNRIFVDTKKEAIILPIFGNMVPFHISTIKSSSKNEEFLRINFNTPTSFTQEQIDAGLIPPQLMYIREVTYKISDPKALSNNYRAILDLRKRVSTRETEDREKRTLIAQEKLILRGAGRAPRLTEVYARPTFGGARRTLGVLEAHENGLRFSPSNTKDKTPIDILYKNIKHPIFQKADQESMAVVHFHLFDAIMIGKKQTKDVQFYTEISEMSQSLDVSSRSFNDEEEEERRERELKRKINLDFQTFIKKVEEIAGAEFEIPYRELGFYGVHNIANVYIQPSVHCLLSILEAPFFVLSLDEVEIACFERVIRSLRNFDLVFVFKDYSRKTIRIDAIPREYYETIKEWLDSVNIKFYSLEKNFNWKNIMDHIRSDLKKFNSDGGWSFLDPQEDEEEEEEDDDDDYSPSESEADSDYISSMSEASSEEEEEVSEGEDWDQLDSKAEREDKRKNYDDQNKRKRDDITSSKSSSSSKPSSSKSSSSSSSSKSSSKPSSSSKPSSSSKSSSSKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UTI6</accession>
    <name>A0A8J4UTI6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nucleoplasmin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003885</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000128">
        <property type="protein sequence ID" value="KAF2074821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UTI6"/>
    <dbReference type="OrthoDB" id="1902587at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.340">
        <property type="entry name" value="Nucleoplasmin core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041232">
        <property type="entry name" value="NPL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17800">
        <property type="entry name" value="NPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleoplasmin-like" evidence="2">
        <location>
            <begin position="4"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="268" mass="30072" checksum="D5406F4E93FD6BDD" modified="2022-05-25" version="1">MMDFLGIEISKEEPIFLDLDEGQIFNLTHAVIHPDSKGTKKCYVVAVVQDGSDSDDEEDFDDEEEQEEEEDQDKEVSVREIPICILEPGKTEQVHLNLLFSSFSLVSFVIKGAEGCIDRISLNGNLETQEEMSDDEDFDGCEDPHCSEDHSQGMFDDDEEDEDEDEEDMIIPELVPIKKAAKGKITEIKEPKVVPQKPDTAAANNKKQQPQKKEKAPVSEKVPEAAPQPKKQQKAQPVAQSPKQKEQPKNNKREASAAPQQPKKKSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVK1</accession>
    <name>A0A8J4UVK1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_009934</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000858">
        <property type="protein sequence ID" value="KAF2068745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVK1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006970">
        <property type="entry name" value="PT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04886">
        <property type="entry name" value="PT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035327933" evidence="4">
        <location>
            <begin position="23"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="302"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="309"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="332"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="364" mass="42007" checksum="9D4923F43C67BFC1" modified="2022-05-25" version="1">MFKQILFIGFLLSISTLVVVRSDFTDCVVGGWKADTCDNNTYFFNNTDYTIVFNNTKIILPPLVKIIITGDAVFRDDAIVHYNCDPYTLADDCIQIIIGGKLLYQEKYMLVDMDLSKYLDEYTEDEYFRIIQFRNTSVDYRNSTYTYPHLAYVNITTPPNSPYNCINLRYPHNNAYDNIVFITCKKNEPKVYDIAHDYNITFSSPCLDIYFHENFGADATYRLKMNFSDIYVARTQFLTAKSLSWVYFRFYAYFGPQRPDLTEHFGKNKTRMDQFANYCWENPDPTQTVTITRVYPYPLNGPPNLTASPTNQPTNQPTNQPTNQPTNQPTNEPTSPISGEITPSNSMVLKPSIIILLCLFLAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVZ7</accession>
    <name>A0A8J4UVZ7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009483</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000721">
        <property type="protein sequence ID" value="KAF2069193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVZ7"/>
    <dbReference type="OrthoDB" id="17429at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035320677" evidence="1">
        <location>
            <begin position="19"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="154" mass="16208" checksum="4A7CD9CFC3F9C7B1" modified="2022-05-25" version="1" fragment="single">MKSITLLLVLMGIALAAAGYSCPKPCYGNMCCSVAPGNLYYLTDFCGAQSACGPIPSCSATSYFTADAQRFGCGKYLNVCNSSKKCVKAAVIDSGPAEWVEQDAGRMIIDGSPSLCRELFNVGSCGWSDHFSITATVASLTDTRPLGPFNVTDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWA4</accession>
    <name>A0A8J4UWA4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 15">E3 ubiquitin-protein ligase listerin</fullName>
            <ecNumber evidence="5 15">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">RING-type E3 ubiquitin transferase listerin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">CYY_009292</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">E3 ubiquitin-protein ligase. Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the ribosome quality control complex (RQC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the LTN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5 15"/>
    <dbReference type="EMBL" id="AJWJ01000673">
        <property type="protein sequence ID" value="KAF2069388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6108at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16491">
        <property type="entry name" value="RING-CH-C4HC3_LTN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000038">
        <property type="entry name" value="E3 ubiquitin-protein ligase listerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039795">
        <property type="entry name" value="LTN1/Rkr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054477">
        <property type="entry name" value="LTN1_E3_ligase_6th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054476">
        <property type="entry name" value="Ltn1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054478">
        <property type="entry name" value="LTN1_UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039804">
        <property type="entry name" value="RING-CH-C4HC3_LTN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12389:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE LISTERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12389">
        <property type="entry name" value="ZINC FINGER PROTEIN 294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22958">
        <property type="entry name" value="Ltn1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22999">
        <property type="entry name" value="LTN1_E3_ligase_6th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23009">
        <property type="entry name" value="UBC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01197">
        <property type="entry name" value="FANCL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 15" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 14 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="1631"/>
            <end position="1676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF2069388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1680" mass="191709" checksum="FB6E0CD0C8EF3F85" modified="2022-05-25" version="1">MGKDKAKNLRGPKYNPSSSGEAGTLMGSGSGFIGFSAFSTTLTNATDPDYYQVDPQYQVLLKKLQKKDSTSRLKALEELNKLFADINIESADETSLLSIKSLLHAWEFMYKRLVNDDDRYVRDLASLCLGTLGSRVGKHLGAHMKTLLGPWLFAICDRKDQSVNTALQSFNNIFPEKKRKDVLVFGHDKAIEYLCENLQETPSTIGDSKSIAPEVLQERYERCISNSLLAIEYLIDKTTATTDNQDNTTTTTTTKGVSNEVYENIFQAQFFTFFSSKSSLIRKTSYRVLTTIINKVPGYVDKNFKEMSSKVLGIFSEKDSSTHLYMWEAIISFCKRYKDQAWEGVDARKHVLPRFWALLRSGCYGSYELSYPSIFPFLTFIPSTVIGQGTGFFKEFFTNLFKGVEILDTYKQFANPTTAAANINAITTLKASDHLLVSYLECLIYSCKKYGVGSDKEVNDYLLNGLLENLIIDFFTLENSSWTEGVFINQVWTTMVKLSSFGQENKDALRVLFDNLEKEIEKSLFVANRDESKQDTAASPANLTKVKPIQVDGLLHRLSTLFSIANVQDDQLPFVGEFAQSLFKECCKYLENGQQQQTNAIKCISTIICHYSKVLSGTTIDDSFFNNSIIPSLKQSIEKQDTELINSLISVIAKIFVPQSFDKILSIFLSNGIDSYVYLISLLKNIHSREKLIGFATLDSIIESSIQIEVENQQHHHIIKDLLSVVLFNQYQEYDCLVSEKTLNTVFKWQLNQVQLNLNAILNFPSLEINILSLESFSMLLFSIPTEKQTADQVAIIENLVHDWASLIVYLCHIDCAKYSQSLVSITNSIFKSKYNNCLISNSILSILFKFILNNNYSNIKLLSELSIHLLNNNNNTLVYLFELYEKSQIIQDGIKNVRFSLVKYNITPFNKSLEHCAQNNRSSTDLNLFLKIQQYILITIGSFGFEKVLKTLSHDAIISQLLIGLVSLDSEIIYPINQKTSHDIDDLESFINNGGYQEIKNQFLTSLISSSDLAQQKWFGFMLEKQNTSEYSLNYSIVLSVLFNFINDSSVMQQETKLTILNNLSIDTSSSVSSFKLNLIQSCLPLLVSVKPQLVEEMSNALYKQLLDLPLSIPSTFEPIGISISLLSSIISVCTIIQEETYTINQEQTHILLKTLSTFQQWYHRLLSPLMKDQDNDFEKQMSTPLLTYLLTVCHSIIAKDIYLSANQSLLVKSCLELWSKHSGIDSQYQILIEYQSLKLFNFIISKENSKYIIPVQLYEEFLLNKAGVYLALWISASTKANKIGYGFLSLEIESLLELYLHCFEKIPKSILYKLVVGSDKLDQIFELALFHHKYIQLTSVCLVESYFLETKTEIVRVIEINEDQDPEFLFFSPKFESILLDFELWKQLKNNRDSKYLLTKAIGNLMLWDILMKHYSIQDISKKSNITDYLNVGYKVSTLLSNIFTFAKKDGEIVFKDVQEFPYPNDDINYRQLQPLCSFSIFSLVNYLPSLVRRWADGCSTKITQQVNHFITHYISPYIIRNEITRVNNHKEGLDSTFTVKGSTSTREVIASYEKDEMTISLVLSIPETYPLRILTVEFSKRVGVSENQWKKWLLSMTSLLLTQDGTVLDACLLWKHSLDKHFEGVELCPICYSLFHNGNIPKFQCSTCKNKFHSACIYKWFSTSHKSNCPLCQSQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWK1</accession>
    <name>A0A8J4UWK1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_008903</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000596">
        <property type="protein sequence ID" value="KAF2069780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWK1"/>
    <dbReference type="OrthoDB" id="5511210at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="5">
        <location>
            <begin position="58"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="20"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2069780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="174" mass="19787" checksum="167E33BA7D825BDF" modified="2022-05-25" version="1">MATFYNLYDLINYLEKTKENDSTSKNVNHQSNTPQPTSQSSNNNVQSSTKCNNFKYQEREPSFEPNVDLYETKDTVYIELEVPGVEKSDINIDWNSNASKLVVSGARKKNELLSNNDSKLLVNELEYGNFKREIKLNSNLINLPSINANLNNGVLLIKISKKPQDSLNIKVNIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UY40</accession>
    <name>A0A8J4UY40_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Cysteine dioxygenase</fullName>
            <ecNumber evidence="2 9">1.13.11.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_007292</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-cysteine + O2 = 3-sulfino-L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20441"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:61085"/>
            <dbReference type="EC" id="1.13.11.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="9">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the cysteine dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.20" evidence="2 9"/>
    <dbReference type="EMBL" id="AJWJ01000380">
        <property type="protein sequence ID" value="KAF2071398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UY40"/>
    <dbReference type="OrthoDB" id="543511at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017172">
        <property type="term" value="F:cysteine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019448">
        <property type="term" value="P:L-cysteine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10548">
        <property type="entry name" value="cupin_CDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010300">
        <property type="entry name" value="CDO_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12918">
        <property type="entry name" value="CYSTEINE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12918:SF1">
        <property type="entry name" value="CYSTEINE DIOXYGENASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05995">
        <property type="entry name" value="CDO_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="3 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0883">Thioether bond</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="cross-link" description="3'-(S-cysteinyl)-tyrosine (Cys-Tyr)" evidence="7">
        <location>
            <begin position="107"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR610300-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR610300-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2071398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="234" mass="26947" checksum="1D095B1CECA4785C" modified="2022-05-25" version="1">MTQENQTSPDIRQNGANTKTPLEELVENLHKEFSIMQEEGYYGNTEAITDLMNEYVANNDDYKDYCFSCPYKYTRNLIAKSDVFELMVICWKKGQPSPIHNHEEQRCWMGCVKGRLQETYYVFQDTKDTHGTGLLEESHCHPIENGSVGYITDEIAFHKIESLTEDSTSIHLYSRPITECNIYCPMTGAITRRKLGYFTQYKSKESINVHTSFDYVPTTNSYISTNTTAISTTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UY77</accession>
    <name>A0A8J4UY77_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carbohydrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006431</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000296">
        <property type="protein sequence ID" value="KAF2072255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UY77"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035210496" description="Carbohydrate binding domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="151" mass="16868" checksum="4CD8C6066BBA7D09" modified="2022-05-25" version="1">MKFLTLFATLCLCVALSSAYYSPPCDGHCAYGETSVCYHDQPAVCLGHHRVVEQVRLRIQVTSVFIEDGISYKSIQVEIDNQSPGRITHIAIGTEYLQIKDTVSIRNVQQVENDFILPDGQVIETGLSYTFSCTIKDIHNPSVYIKNVIIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYD7</accession>
    <name>A0A8J4UYD7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_006119</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the steroid 5-alpha reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000268">
        <property type="protein sequence ID" value="KAF2072570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYD7"/>
    <dbReference type="OrthoDB" id="540503at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01801">
        <property type="entry name" value="Ubl_TECR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039357">
        <property type="entry name" value="SRD5A/TECR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556:SF28">
        <property type="entry name" value="VERY-LONG-CHAIN ENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="251"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="11">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2072570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="301" mass="34582" checksum="F586B1AA29E8E0C9" modified="2022-05-25" version="1">MNITIASPRSKKTICEVTVDPSSTVADLKKKFAQQKKVGVERQRFSFEHPTKGMVVLSNDKETLSNYGVTTNTTVLFKDLGMQISWTLVFLAEYAGPLFVYPIFYFFSRQIYGFETPKTFVQDVALICYSLHYIKRILETIFVHRFSKATMPITNLFKNCIYYWGCTSMVSYFVNHPLYTPPAALNQYIGFGLWAFGEIMNLVCHIQLRNLRPAGSNVRKIPRGLLFEFVSCPNYTVEILAWLGFSIMTQTLTSYLFTLLGAGQMYIWACGKHRNYKKEFTGKDGSPLYPKNRKIIIPFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYX3</accession>
    <name>A0A8J4UYX3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Symplekin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_006522</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000305">
        <property type="protein sequence ID" value="KAF2072163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="19250at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021850">
        <property type="entry name" value="Symplekin/Pta1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032460">
        <property type="entry name" value="Symplekin/Pta1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022075">
        <property type="entry name" value="Symplekin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15245:SF20">
        <property type="entry name" value="SYMPLEKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15245">
        <property type="entry name" value="SYMPLEKIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11935">
        <property type="entry name" value="SYMPK_PTA1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12295">
        <property type="entry name" value="Symplekin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Symplekin/Pta1 N-terminal" evidence="5">
        <location>
            <begin position="99"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Symplekin C-terminal" evidence="6">
        <location>
            <begin position="1107"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="329"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="485"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="835"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1312"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="485"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="530"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="560"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="845"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1315"/>
            <end position="1331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF11935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2072163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1331" mass="151740" checksum="2AF2BBBF661D772A" modified="2022-05-25" version="1">MTSARDTILDILNEASCSEKNRSDRLANAFELLFHKEQSLLDEYYPSLIEFALDRSNSIKKQVIPYIEAICKRYPKFLIQSENTLSFLLSNASDQGGRSIVKRTILCLTNIIRSSLTFIVYQNQSPQQQQQQQPIPTFEQQQAVWKSYNELKNKVVKFLTDDDESVKINVFKFLEIVILSYSSPGEYTSKKYKSDDEFSLDKVPFDHQIISKKQLTGECQVHLNDLLDSAKDLTNMTSANIMILVTSLTTISRQRFSLLPIILPVLSGCPHILPKLGTVQNESIKHSLKTCLLSIIRLKNQIVLPFLPSLIEALAQIDAKEQGEEAARWYRGEPEKNKQKRQYNIDSQEQNKRLKYNDNNQMNDNYNNNNNMMGVNSNNNNNNNFNNSPPMGFNNNNGMGYNSNNINNSPPMMLPGLNMDQGTQSKIISNIPNIRGDLITNFVLENMMHTPLHHLLQSIPLNQKSEILYQFVNSYQSNIQPLMFNNQPQQQPFNTFNKPQQQPQFNSNFNQQQQQQQQQQYGFNKSPVLQPQQQPMQFQPQQQPQQQQFNQPMQYPPPQQQQQQPPQEQQQPLMDDGKDEATEFELTEFEPELEPELDIENLVKPEDLNNIKLERERELQLEKEKEKEREREKESMAMVSAGGTIKSEIVQTITPRPVFSVVKIGSSQAEKISDSAVKRMKSAENGAIIGGSHKLWIGLLSKVVAIKPTTKEIYEEFIDFILKDFTQHRELALQWLYNEFFIAREISIEDSASGGGSDSMSTSGDEDNIKMSRYSNILMNIVEKMIKTFDPKERLLSMFILDLPYVTDLCLETICKVVVNFKDQNESFSVASATNGIEVKKEEPSDQDTGEGDENSESQEPKDRSEWITLGLSTLRDLVFWRPTVRSKCLTYLLDFAVHLDDDLRMPSIRLVTNQLYCKSALAETIQLFAKSQILDLTAFNQDSILSQEDAPVAKPKEIDASAQDQLESPKDQEMMGQEQPVDIDEENQDILNQLKKESKIIKYIERKLFLFYSLCTKNHSLITYLLEVYPKCEKIVQEVFSRLIGNVTKSIGQTSESLLMAISICPSGAEPLLIEILGSLVEVQKPTAELVSAVKRLMHTSNNQLFLLPILHGLTKDEIISKLPVFISLSSSEDTKKFIAKMASPGSPISPSELMVQLHLIPDQSKKVLEAIDYCFELKAIFKQEYMAVTIQQLLVQTPLPLLLMRTVLKTLSVYPRLKSFIVEVMSQLVAKQVWNYEKPWLGFLLCAKNAKPESLEVILQLPTPQFEMAIKKDPELADFLIRYLKPKPPSARTIYGRNIKLLGDIPFEKKVKSSTSAASSTTSATTPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-06-18" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZR6</accession>
    <name>A0A8J4UZR6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_005255</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000204">
        <property type="protein sequence ID" value="KAF2073438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043359">
        <property type="entry name" value="GLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056436">
        <property type="entry name" value="Znf-C2H2_ZIC1-5/GLI1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718:SF4">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23561">
        <property type="entry name" value="zf-C2H2_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="495"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="887"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="916"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="27"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="143"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="240"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="319"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="710"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="786"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="951"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1013"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1065"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1204"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1312"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1357"/>
            <end position="1469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="143"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="156"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="200"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="240"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="267"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="336"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="383"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="743"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="811"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="986"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1079"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1119"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1149"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1211"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1264"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1312"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1335"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1357"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1449"/>
            <end position="1469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2073438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1469" mass="163998" checksum="D3222E6D9F7F6780" modified="2022-05-25" version="1">MLTHSPSSPGGIYFLVDLSNEFNNNTNNNNNSSINNSFDRINTSNNNNSTNTNSNHNQQQQPFSLNKITNSNTSIPPVLSSPSKLFLSTSSTGGTPKQHLISSTSSLTSIFVKEKDIFNINSSSSVNINSNSNLDKSQDILKNDKEKDEKNIKDDNDQDDNDEDDDNIDELKIQEDNSNSKSSSSSSLKNSNSKVSNSNIADQDTVTTDSHNNSNNKTPSISLNLSTELKDKLNNIWSNSLNNSNNNNNNNNNTMASSPVPYFPTPSINNNSNTNNNSNSGLNLSSNTISSLTKIAAAVGVGLDTLLITLSNTKQQQQQQQQQLQQQHHHHHHHQLQQQQQLHQQQSLLGISPSLSSVKMSSISEAINSPNYFFNFDQDFSHHQQQQAPQQTPTSVATSQQQQDFMFLLNNLHNSNNGSLFSTSNNNTSATMAQQFLNTPVSSTSTVSSAANSLSPFNNLSPANASATTANVIVPSLVSPSVPLAVSVPEMPESLICKWNSCDLAFETMTEFWIHFRTDHFKNKPTINIKNGGPQSFVTCDWDCCGMVLKDFQSLTSHIKFHHLLDPDDLESANRTLSRRKKLNSISGHMDPYMDVIGATIGKSFDTNILLSSKNNNSEKNSSSGTGNSGGGIKSVDELINSHINIVNNNHMKTKSFFNDSNAKENYLGDDEPWNKDKYSKTTKLVESLSSKLDNNNNNNSNIHHLHNLHHHNHHHNNSSLPELNDDNYYRQQNHGETIGGHYNHHGHSSHQHTHHGHQHHGHNHHHNNDHMIKKEEDDEMKSTIVHSEKYKSTNIKRKKANDTEMEFMDSSNTTTANTTESGTLAPPTNRKPRKRKSSDPPGPFECKWADCNLGAVFTKLSDLGEHLAEHVRKQKGKKSESYCEWVGCNRADKPLKSAYNLIHHIRYKHTGEKPFSCDFPDCDSRFVQLSDLNEHKRNIHKFEDGYVKKKRNSKKMEGGTSMSIQSNSAEMKAEKKAKKKHHGLISTSPLQDPSMNGAYSRAINGTTNYILEINQDDPSSDPSHQQHHHYYQEDFSSGPSSDGIEHAQQYHHHSSVANPNVLNHHHQQHQQQYYQQQQHHKHHHHHHHGEMEESDQHIVGSNTHHHHHSGSAQNNFAQPTTPSQSSHQPTTPSYYIAKTPTQIHPDDSTTTTTTTTSTTTNSIHSEYVSNHNQNLYSANFPSSPMQDIKLYSPMLPFPSPITDLTDHNHLTPSNNFQPDSPQISYKPRSNNNTSNGDHFVNISDHPSPLTYFPSSPMVPPSPSTSYKPKSNNNSGKTSNSTPSTPLNSSSSKDKPTLPSIYSSLLDNIRESSTPNIMNHNSTDLSIPKNKSKLKTSTTTTTTTTTQVVNEEKTVTTTSTTISATPTPTLISNESENKIENKEINNSNNNEEKSNTQELETPPQPLYKEKEQEPISLPPLKIKNRNVNKRGATGFIAEEDGSIRFNNDQDNDDYYNDDEDDDDMSDTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V065</accession>
    <name>A0A8J4V065_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">G-protein coupled receptors family 2 profile 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_009860</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000832">
        <property type="protein sequence ID" value="KAF2068822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V065"/>
    <dbReference type="OrthoDB" id="100006at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15040">
        <property type="entry name" value="7tmB2_Adhesion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022343">
        <property type="entry name" value="GCR1-cAMP_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022340">
        <property type="entry name" value="GPCR_GCR1_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053231">
        <property type="entry name" value="GPCR_LN-TM7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45902">
        <property type="entry name" value="LATROPHILIN RECEPTOR-LIKE PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45902:SF1">
        <property type="entry name" value="LATROPHILIN RECEPTOR-LIKE PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02001">
        <property type="entry name" value="GCR1CAMPR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02000">
        <property type="entry name" value="GCR1PLANT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="148"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="196"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="226"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="9">
        <location>
            <begin position="10"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2068822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="327" mass="37163" checksum="0E1D8DE03B7CA4D7" modified="2022-05-25" version="1">MGVDEVYKPLGYIVDVFLALSIAGSFLTVFTFMLFHKLRTYPIRLILNLCMAIFFAQLFFFISFKSFDSMFCLTAGMLLHFFFLADFIWTFCVAFNFYQMIVKRNRDVEAFEKYYHIASWGIPMVIVVFCAGFGQYSNRGNFCYLNEAVPVFVAFFIPGLLVVCSNAVLFFFIAREIHDTLKNAAKTERKDKSKQFRVYFSIFASIGLSWIFGFVMTFFSEGVLNVVFLVLFSVTTPLQGALIFASYCLNDKVIRSYASLFSKIIPFCKRWENYGTSIQTSSTQNLSTVLGRSSYINKSSSSLESDIPMKNLNSSINSNNNAGPGSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V093</accession>
    <name>A0A8J4V093_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004583</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000165">
        <property type="protein sequence ID" value="KAF2074098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V093"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="76" mass="9074" checksum="A31F288E9553EC91" modified="2022-05-25" version="1">MSTRNLQYISERLQQDNINVLRVDCDKEKQLCHDFNSDQHTHNHMKLVLYKNFVPIEVPDNLGYESFIEFVKHAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0J1</accession>
    <name>A0A8J4V0J1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Bacterial surface antigen (D15) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_002947</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000087">
        <property type="protein sequence ID" value="KAF2075765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0J1"/>
    <dbReference type="OrthoDB" id="22634at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019867">
        <property type="term" value="C:outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.50">
        <property type="entry name" value="membrane protein fhac: a member of the omp85/tpsb transporter family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000184">
        <property type="entry name" value="Bac_surfAg_D15"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01103">
        <property type="entry name" value="Omp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bacterial surface antigen (D15)" evidence="3">
        <location>
            <begin position="206"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="404" mass="45744" checksum="A449C8CC779B9415" modified="2022-05-25" version="1">MVRIVFDKEKPLNSSDEYLLSQYFSQLDQFKDASVSNKKVQDYISRGMDKMQQEGIFYDAELSVDTNTNTITVKPTSRMNFRIGATTDLKQEFCYNTANPFGKGGTFEFNVEMDLKSVIGKLQYSDKTGNTIMGTRTTEKSSYKIKEGFNIDTSSLSWLWKKNRHSFVIKTSDRSVGVDPSNHRKDHLASTGSSYLLSLQHDFSFKKVFGAYQHFGQINSELALPYLSTFSFLKSRINFHQLLDLPFRMKLFFSHSMGGIYKIGNNNTLTPVPDRFFNGHSFVLEGFRDNSLVGSRGGSPYMGSDLFYTSRISLLKSIRDEAYVLGFYSFGNATMDYNSCTSGCGISKSAFEDLFNPKSIRSSIGAGIVFPTNMGLLQFYFVKPLTHFKKDKLNAFGFHATFSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0S5</accession>
    <name>A0A8J4V0S5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_003946</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000131">
        <property type="protein sequence ID" value="KAF2074758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0S5"/>
    <dbReference type="OrthoDB" id="30818at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041653">
        <property type="entry name" value="Importin_rep_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057672">
        <property type="entry name" value="TPR_IPO4/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18808">
        <property type="entry name" value="Importin_rep_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25780">
        <property type="entry name" value="TPR_IPO5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="IPO4/5-like TPR repeats" evidence="9">
        <location>
            <begin position="102"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Importin subunit beta-1/Transportin-1-like TPR repeats" evidence="8">
        <location>
            <begin position="459"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2074758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1066" mass="119976" checksum="B33BF319A820681D" modified="2022-05-25" version="1">MEIPPDLINTLVGLASGNNDLIKQSENVFKKFKTESPDVLVRSLILMMKIADDQPLLKEYAPVLLRQLIHPNAQESVWEKLSLETKVYLKDQLIERLQLETKPSILHKIVTLVSTVASDLIEKGIWPDLLQFLIAGTTSQIEHLRESSFSILGTVILDLSQMITPQTYPTFVQVIENGLKDPSLKVRIAALGAINSFIEIDADNSVQFKKLIPNMISTITSALEVSEEKSAQNSILSFILIVEANSNWVKDHFPLIYQTFFEIMISPDFEEETRHFCVEFFITVAENKPSLIKQPQYLAPITQKLLEWVTTVEDVNLGEWDKSEGENDPEELPDYSVALDALERLSFYVPKNLTDQVFPILNSLLQSPKWNQRYAALIVLTMIADGCKKSLGPHLKSILEKILGLANDEHPRVRYALFYCLCQLSIDFRSKIQKNADHLIPLALRRLGEEKSRRVVVCICKFLTEFLDDIKSEIAIKFKDQFFGTLEPLVTSQDIIISQDALNTFSSVIDGIGAEFTPYYDRFMPFLFNIISKNGKEYATLRGRAIETISLIGLAVKKEKFAPYCQQLMLHLKDQPKFANDDPQTDFFLRAYTRFCQCLGEDFVPFLPIVMAPLVDALTNEISISTEQFSTDFVQDSIASDSTMAAENKALALHLLSIYAAELKRHLAPYAERLFQETLKLINYKYNEEIKNNAVEFLPQITKILKSDCEAKGDNSFGPIKIVFEHTIQHLIKSLEQEESIDSISIKLKALCEMVDIVTSNVTHYLPEFFSVINETLQTLVESDKVYTENLDEEDDDQQESYVKDSLDEGFNNIAILAGDICIHAKEQAVGLMSNIIPTIISLIEGSRAEIKSSMICIFDDIIESCGEPGFSLFPHIIPPIMASCLPNPANSSDVLQSAAFGLGVAAQYGKEHFASFAFESIKVLSALILRPDAKSEDNITSTENAISALGKIISHLPHHLNQYLPELVSQYIMQLPIQDEGEYGSSINSLFLLMTNPESSKVIFGSGDLFIKSLVSLAEAHKKKVVTAEFHAAIKQLFSDKTPQLQEVFLHLNPNERENVKELLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V152</accession>
    <name>A0A8J4V152_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008564</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000540">
        <property type="protein sequence ID" value="KAF2070112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V152"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="84"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="344"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="115"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="344"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="355"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="374"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="389" mass="45032" checksum="A2F8A8CFBE0B639C" modified="2022-05-25" version="1" fragment="single">MYMYQPQQNEPHLEQQQQQQQTIINHQKLNDIASAPTSTIDMNNQNIYSNSATYQNFDNSSSTYNQFLQEFQTQSINWNSPSTDINSAMISPPNMPINSPPIPSLATGSNDQNILQQQQQQLQQQQLLQQQQQQQQLQQQQQQQQQQTIMSNPSIQPQQQQPITLESIFNSIPLPPGHIPSTQYHQYFSEKLAEKNIPYETILSLYQQTEIFNQMITNRFTIELNSLNTQINEIHTQGTHLQDPALYQNFQNLQYTYIVRYQQYQQFKFPDQSSQILIQDQQAQQQQGHQIQQVQVQQQQQQLMQQTGQWMGNTDQQQQQQVQPIIQQQQQQEAAISQQLQQQPIIQQQPSQQSTIPLASSTESIPNTKKAKTSKGTSKASSSSSKKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1E7</accession>
    <name>A0A8J4V1E7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_008267</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000495">
        <property type="protein sequence ID" value="KAF2070412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12087">
        <property type="entry name" value="TM_EGFR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1280"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="5">
        <location>
            <begin position="403"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="4">
        <location>
            <begin position="630"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="3">
        <location>
            <begin position="768"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="2">
        <location>
            <begin position="1051"/>
            <end position="1253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2070412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1319" mass="145820" checksum="22D5BB65A6F353FA" modified="2022-05-25" version="1">MHNVDVSHALKATVIGFIDINYYANTQNKCTGRQIIMQVTEIGSDPITGILPSIGEFAPNPAILIENSTTVVYNVLVTLYSNPAPYFIDVHFTLTVDLNPVTVPLLSTDGQPYYFECILVPHTPTQYEIVQPLKLSTTVSKSTIFDGYFKLDSDFTRGLPDSQCNAVDTSSVSIAICSILYVGNGLYKTSFELKNSYPSDLTSSTHKFTISFLGFTFLFDNPLANTQYQNQAIYLNVQQVQANSWLAGAVFNFNDVQDVMPIISFAFRPLKGNFKSLYLFIASGAQFYFMASKSFILSPSIPSTTTYPQITNSLGQSQTNSWQENTLYYVISSFSTNKTYLFSQVNPLYVNTAYPFGLVSVSGAALPSYKISTMFSPYQNLYSINTGLSWTQNFPITIVGSINTDTIAPLLKSISIIALNTTHSIFSLQVTDNISGVAFIQVYQRSLDKSISLNQQDLVSGTLLDGIFEKILPFQKYSTFYIQISDHAANNVLIDQNALFLTYGVAGLFTEYDLYGLSHLEFKMTNVDVSSGSVTNTLYFNFTLPKGYQRKYTNVQFNPTFPYRNGLQSEYFGKYNTDTQLYEIDFVVPAKTMTSELKYSLFINGVLIQPFLILSKFYSTSRVFITSSNFDQMFPVVTSITASPASLDISAASADISWTLYFSDENFVKKAIVQVVGDFDLEGFNTTVIPTTLETNIQVLITKPLDPNVSCRTQNYYIKYIYTEDSLGNVGETFRDVNSNIHPYYLFDSSATDRIQVTCTRAAGGQTPVLVDVSIKQTFNLTTKIPTAIVNFVVSGTVGPSLPSCYFHSYPMDFLSVKAAIVVKDSIVKMTNYSCTIQLPFKYGPQVGVSIYGIVDSFGQYLGYSTNELPATENQFTNIVNSFKGPLIDSVLRDGTKIGVTGTQLYKGEIFVYVDDSPAVKLASVDIATGIYILAGDFTPPSNPSSSVYVSVYNSDISQESNRLLLIKGIPPVTPSPSPSPPAPCNLNCGEPQGHGKCVNGACICNPPYNGLDCSSKTDNSTVITPDPNKPTVNVTIPGTSSGQTPEFISFIYVYGVRELDNTDTLINNYVFNSDKWILVKEGSSSNEQVTTLQYKYVIDNSLNTTIVSTVQVFAQATNITFGNQQLYMNPSTIKFTFNVTSYPFSKSTNSLQLVMTAALESTEKVACSYKEFVGDQNNSQYLKIQIQDRSLFGRFIKFGMIDGREQVVSNTQLDNVYGGKELSKSTSDQSYIGLNIPYYTKYALLDPDFSVLVEQNTARDQANSICTNKSSKKLTNAQIAGIVIGGVVFLFIIGAVAIYFYTRKSESTFAMKLRKMAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1H4</accession>
    <name>A0A8J4V1H4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010532</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001175">
        <property type="protein sequence ID" value="KAF2068144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1H4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="163" mass="19400" checksum="F885959781053A59" modified="2022-05-25" version="1">MSNTDDNEGCKVSEDDDILSNIFRSSFSTKERDSNFIDNTLQLPNNYNNNPYNREYKSLVGQKDHDNRIKYYLNRQKEKRRSLLSQVREIISLPVEEEDDSRSNLEIQQDIDMEENQEYDQNTNTNNDSNNNNNNNNNNNNNNNQEKKKKKKKKKNKNQMQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1M5</accession>
    <name>A0A8J4V1M5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">S1 motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_008097</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000471">
        <property type="protein sequence ID" value="KAF2070592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1M5"/>
    <dbReference type="OrthoDB" id="1685042at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04452">
        <property type="entry name" value="S1_IF2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1130:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000015">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1130">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.190">
        <property type="entry name" value="Translation initiation factor 2, subunit 1, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044126">
        <property type="entry name" value="S1_IF2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024055">
        <property type="entry name" value="TIF2_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024054">
        <property type="entry name" value="TIF2_asu_middle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011488">
        <property type="entry name" value="TIF_2_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07541">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110993">
        <property type="entry name" value="eIF-2-alpha, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116742">
        <property type="entry name" value="eIF2alpha middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1 motif" evidence="5">
        <location>
            <begin position="19"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="306"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="306"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="335"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2070592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="344" mass="39331" checksum="6909ED008C0E0B72" modified="2022-05-25" version="1">MVFTENDCRMYEKKYPEENELVMVRIESIGDMGVYVSLLEYNNIEGMILLSEISRRRIRSINKLVRVGKTEAVVVVRVDKEKGYIDLSKRRVTPEEYTQCEERFHKSKAVHGIVRYVANKLSTEEAPVKTTYLYKKFVWPLYAKYGHAYEAFKLSITEPSVFNGFDIAENEKKILQDTIVQKLKPQPHKVRADLELTCFDYEGIDAIKYAINASQKFALQSVVIPEGQELEEKAFGVVTIKLVAPPLYVMVGTFDEKEKGLAMVNRCVEVLSEEITKKKGALTVKVAPRIVGAVDDQELRELMEQLEVENQEVDGDDDSDGYGEEEEEEEEKPKKSSKSKSSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V267</accession>
    <name>A0A8J4V267_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UBR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_000709</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000014">
        <property type="protein sequence ID" value="KAF2077985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V267"/>
    <dbReference type="OrthoDB" id="427974at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19671">
        <property type="entry name" value="UBR-box_UBR4_5_6_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006633">
        <property type="entry name" value="Carb-bd_sugar_hydrolysis-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007742">
        <property type="entry name" value="NosD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022441">
        <property type="entry name" value="Para_beta_helix_rpt-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051550">
        <property type="entry name" value="SCF-Subunits/Alg-Epimerases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03804">
        <property type="entry name" value="para_beta_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22990">
        <property type="entry name" value="F-BOX ONLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22990:SF15">
        <property type="entry name" value="F-BOX ONLY PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05048">
        <property type="entry name" value="NosD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00722">
        <property type="entry name" value="CASH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBR-type" evidence="9">
        <location>
            <begin position="703"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="7">
        <location>
            <begin position="703"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="221"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="798"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="252"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="802"/>
            <end position="817"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2077985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="817" mass="90936" checksum="608ABA93CD10C9A5" modified="2022-05-25" version="1">MGHFSFEQFKQQLQQQTTQNQQNQQPPNPSTLGDIRMAGGGGGGGGSSAPEVGTSINNFASSSNNNNINNNNNNNNNNNNNNQDEKSFEYKKYLSTENLIDIIRSSNLLASPDSNSHLEILSPDTSFDCCQDKEFPKSTPFSEEHWMLIFQNLDSKSLVSLSATCRLFYRLSHDKFLWSNLLAKDRKSVLLLPNGIDLEEPLEIYKNRREFDTIKEALDAAAPAKKKEETNGGEANVNNSNEGLPTTPTPTPNNNNNTNNTSSTNSAQQIFDPKLRETILLAPGIYKECIIIEKPVDIIGQGLNTEVIIESSSGNTVTITAPTGSINNLSMRQSGHWFCIDIEKGAFTISNCDITNSTLSAVKIGKNASPWLANNIIHDCKEAGIAIFGGEGTIIGNHFTRNRYGSVEIVYSTAKPIIKLNRIYKNKGYGIHVHTNASAIISENIIEENESDGISCWGGASPIVTNNKICNNLEDGIYIHEDGKGVYEYNQIFGQKLDGIRIAKSNPHIAHNTIHHNQGDGIRLVIRANPIITENYICENKRVGIHIYREGMGIITNNYIYGNKNAGMQVYSRANTTISNNKIVNNRCSGIYVSDYAIVNIKDNEVSNNGEVGIEIVSGAKALCFDNNNINRNWETGLAYYIDSKPIGFESTNLFSHNGTGGSSQFMMRQGREIMMYNARKAKNNSEHFEPISFLVENALQEGLCTLSFTREYYHAQYWYECKTCSEPRKILGKSEIAVCEECAKKCHAGHDIGVRKYGHFYCDCGQQIASPCKCISISPQSATTLVPYHSVHQYQQQQPQLHHHRHQHPTPQRVRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V291</accession>
    <name>A0A8J4V291_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Myotubularin phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_000626</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000012">
        <property type="protein sequence ID" value="KAF2078075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V291"/>
    <dbReference type="OrthoDB" id="271628at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14507">
        <property type="entry name" value="PTP-MTM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF59">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myotubularin phosphatase" evidence="5">
        <location>
            <begin position="447"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="22"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="114"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="275"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="22"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="114"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="134"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="275"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="297"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="2">
        <location>
            <position position="675"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="587"/>
            <end position="590"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="612"/>
            <end position="613"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="675"/>
            <end position="681"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2078075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="941" mass="106805" checksum="75824C438E09BF1F" modified="2022-05-25" version="1">MNSRLPNNIQIPVLMTQNSLSALLSPSNSPRGSNSNYNNMDGGSSSGNNTNNEKLDKLKDITSKTIDQVIFKLTEMKREKQFLQSFKDIMKISETLIEIDRTLKPIQITEAELSRTSSSSSFSSRPTTPRKDTLTSSGGSSLSGSRDNNNPSIPPALPASSSTLEAELIQIIHNKSNTIKKSKAHDRSTDSIESVHAIIHKCLDLIKAKVVQVKKNLVFEQNPQTALQYGEMIRNIRNQVNTFRTLSLPQNLNLCDGEKLLLKVENCHYHLYDNSHNNNNDSKSSSNSNNNHHHNQKQTSSNSNPNNQNNTPIEDDIVKGTIFVTNYQIIFFGIKSNNNSSNNNNNSNSNLNLHNNNEENIFIKQFSLHSINKWTKSGKKKSPGEYSYKLTFICKDYRNHIISFDKTTTSLKDVRKCIENQRLTTTSTLFCFTLKQDFSKFENTNLGWSLYNAREEYARMGVPSPDWRVSDANHNFFVCDTYPALIGVPESINDEQLKSVAQFRSKGRIPSLSYRHWSNKCSITRCSQPKVGIGKNRSEDDEILLNAIRCTSTAKAESGGSSSSSSSNKDRKLYILDARPYANAVGNRAKGAGWEVMANYPGCVIEFLNIANIHAMRNSLNKIKESSVSSLAKEDSWYSSIESSGWMNHIKLVLQGAIRVAKLVHTQHSNVLVHCSDGWDRTSQLVALAELFLDPYFRTLRGFQVLIEKEWLSYGHKFAQRNGQIHNKDEDERSPIFYQFLEAVYQVVQQFPTKFEFNAHFLRVILYHSYSGKYGNFLYNSEKERIQNHSFGKTISLWAEMDLTIKDYLNPLFVPSTLKERDVIFPDCSLKTLRIWDQIYLNNLSDTEIYGAHEKVVRQVMDKHLDERAEAFRELYPLKQKRSHTASLSFANPRQSMSISQLNLLPSFHLSNTIPKNSGIELENIVEESVDFSENISSTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2X3</accession>
    <name>A0A8J4V2X3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_009467</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000717">
        <property type="protein sequence ID" value="KAF2069209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2X3"/>
    <dbReference type="OrthoDB" id="18798at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00821">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="565"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="45"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="688"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="767"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="47"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="700"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="743"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="767"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="785"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="817"/>
            <end position="829"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2069209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="829" mass="91125" checksum="928509D6316E65BF" modified="2022-05-25" version="1">MFINLFPIRLDLSNSANNSPTGSQSINHHHNNIFNRRNSLGFINKLSTTSSTSSPSSSTSSSPTASTTPKSSLLNTINNSSNSNSNSNNNNNNNNTSSPSGSPKMFQKTITEIRRPSIANIMSNHLNGSTGINNNANTSSNNLNSSLSNLSLSSSIDDQHGSGIIGDVATNNTGYSSDLHNSGIQHSKISEAIDIVVIGDELANKAKFISTFLNNGIGEDPTSIDVTCKKSVMIQSGTYTVNIHSTVGQEDFWGINDVYYRQGAGFIFVYNVHSRESFNLFLKLRDKIVYDKGSDNILMSIVGLNHNEGGAEIREVSFTEAKRMADLYSCSFTEISSFGRDCEQSITGCVSDLLSRITNFQTTLGGSNANSNNNGGSNGGSATNNGSHQQSLEVLMLGDIFVGKTQIVQRLMGNAFNATYRETTDWNKNVYQQTLNDTRYLIKIVDTSGLNIEEQLDRERLVNSQGFILVYSVCSKQSFQLLETLKKKVSACKSDSKTPCILIGNKGDSVVRQVTFDEGSKLAQQWGCQFFEISAVTSEEETIGKAITQLVSDIQKASNNIELGEFKKKGYLLKEGKKLKSMSKYYFKIYKGNLQYCKNENNKSKLKTIELSEHVQVHTISSGEKKDVWPFQIIIDPLKQTHINLIANSEVERDSWVTTIKLNCYVNEAIGNLVDDVVKNMVTEIAMQEKSSSHFKKTDSSSSFPSSPISTPTKHSPSNFHHHHSSNQHQSSPNTLNVNHHNSTASTSPSTPFTPQELKQQLNVLLSNSNNSSNHGSHSNNNESTFTSSLYGDPNQSNEKDTPMSPSLHSSFKKNLLQRTTSFTKGKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V505</accession>
    <name>A0A8J4V505_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_007300</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family. DDX6/DHH1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000381">
        <property type="protein sequence ID" value="KAF2071384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V505"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17940">
        <property type="entry name" value="DEADc_DDX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000114">
        <property type="entry name" value="ATP-dependent RNA helicase DDX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47960">
        <property type="entry name" value="DEAD-BOX ATP-DEPENDENT RNA HELICASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="16">
        <location>
            <begin position="42"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="73"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="253"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="11">
        <location>
            <begin position="42"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2071384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="416" mass="47291" checksum="6ED4466F8E850371" modified="2022-05-25" version="1">MNTNTQKPNNSIIDDDNWKSQLKLPPKDERRQTEDVTATEGNEFDDLHLKRDLLRGIFEKGYVRPSPIQEKAIPIALAGKDIMARAKNGTGKTASFLIPALEKTDPTKDVIQVLILVPTRELALQTSQVCKELGKYMNVQVMASTGGTSLKDDIMRLYNPVHILVATPGRVLDLAQKNVAKLGNCHTMIMDEADKLLSPEFQPLVEQLIHFLPPTRQILLFSATFPVTVKTFKENYLQKAFEINLMEELTLKGVTQYYAFVEERQKIHCLNTLFSKLQINQSIIFCNSVNRVELLAKKITELGYSCFFIHAKMVQAHRNRVFHDFRNGACRNLVSSDLFTRGIDIQDVNVVINFDFPKHSETYLHRIGRSGRFGHLGLAINLITYEDRFSLYKIEQELGTEIKPIPPVIDKSLYAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V517</accession>
    <name>A0A8J4V517_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004533</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000162">
        <property type="protein sequence ID" value="KAF2074162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V517"/>
    <dbReference type="OrthoDB" id="10249567at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14733">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01763">
        <property type="entry name" value="Ubl_SUMO_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24413">
        <property type="entry name" value="SPECKLE-TYPE POZ PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="175"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="354" mass="39989" checksum="71EDF81FE76827BE" modified="2022-05-25" version="1">MNTPTTPNTITTNNSTTPLTPTNNALNTNITTATTTIGGMVSSIINNSTANTTTAAANTSAYSSTSTEHITITILPVGIPIFGLPPFLMKKHTLFRRLFLHVANRFNMDENELLFLFQTRISPDQCPNDIGLVNGDIIVVRSITWKQKLSYESKQSSGSTFVDNIRSLYDSSSYSDICFKLKDDSVLYAHKNILSSRCEKFKVMFKIDMIESVAKELEIKDYEPAVFRKMIEYLYSDDLNEDNVEMVFQLVVIADEYLLDALKKLCESKLISEMKNNNAALFLIQSDRFNCKNLKKASLAYILQNIKKLYGTTDFEPLSDYPTLLAEIIGKMVPFYDDLANGKKEFISYEYNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V670</accession>
    <name>A0A8J4V670_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000096">
        <property type="protein sequence ID" value="KAF2075527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V670"/>
    <dbReference type="OrthoDB" id="10290201at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="520" mass="58907" checksum="7194997CEAF87AC1" modified="2022-05-25" version="1">MWTTTGSSQSFYLVWRNVYIKRCIVIHILRDQRIDVSLDQLDDECFRYLSSISLNEKLENNISVNLDIKSSEQFVQYYNHPHRDLINSLCVYSGAEITLPPVTNIDKFNLLIRSIQQEGERIRSFYCSLIPNTVQSLSITDYNGWYYQGDVKLPDSITELILDSPTDSGNKRAPLLRDILSNLPCKLRELVLPHLNLGSICRLPDTLVDLNYIGDYERLAKFIVPPNKTYMNCSLILDGSFEALEWLKSNKWVTMTSISDTRLDEIPSHVQRIYAGDVVIEPDTISSEVSLVSTMGASIAPGPLPSNLTTLLIYYYDGLLRELVSSRLQELELNIYNQQLEPGLFPSSLTSLSLGSYNSPIKSNVFPNQLKVLKLNEFNGVLEIASLPSSLTRLDLSSFTGSFDSVGPLDSLRDLSIDTMNPSISTLLLNVANVKITLKQITQDTSLSNIPIQKLLLFFRGQDTRRFVLQPQFLPHSIKYLYLKNIDIESNNIVPNTCKYLSTDQLELNKDLLPISVKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6K4</accession>
    <name>A0A8J4V6K4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002765</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000080">
        <property type="protein sequence ID" value="KAF2075917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6K4"/>
    <dbReference type="OrthoDB" id="10657378at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="216" mass="25318" checksum="894C70B8795B1020" modified="2022-05-25" version="1">MMAIETFKILSNNNNCYKKLGGLSIPTSFKNNDFDNLIIKKEDFNNYNNNNNVNVNNRLYNNNITIKKIINNDDEQEFQQEQSFFLNSNNNISNNNIKIENLINENNSNINEDLFNPSNKKYQCQHLNCLETYRWPSGRSHHYKHSHKEECAMGENGTQCLKCVSNRKFKVSTKTKCRHQSCNMLITPKNRSRHERNLKIHSKCPSMCKVCKFPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6M4</accession>
    <name>A0A8J4V6M4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005682</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000233">
        <property type="protein sequence ID" value="KAF2073004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6M4"/>
    <dbReference type="OrthoDB" id="21671at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="324"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="425"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="550"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="823"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="27"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="99"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="324"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="336"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="435"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="550"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="823"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="841"/>
            <end position="854"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="869" mass="99488" checksum="478A66C16388B1F9" modified="2022-05-25" version="1">MSQLNSSGKSEIVSEIERINGILVDNFIKHQDEIKRLNVDIDNLKKNKSDIETKYLSLLKKTGATDQEVAIVQQQQQEQGNDSNHVVNDASDAIASTTATATTNSAADTNTVNELQSKNQQLDERIKELENEIEQLKNSLQDSNSENIGDNESIIEQQKHEITKLLQEVNEQKQSVKLLQVYEFKETKLIEERDQLQQKLEQQISNNNQLKEQLEEQEKKYNDFKAETDSLQLNQVDSSDLIKENQTLANKAEQQSEEITKLTGLVESEKNSNQQLLQQIESLKPLEQQVAELQQEIQNLSLNSTDGQDKIKELESEIQQLKDSAHQLEETKKQEIQQLSDKLQQMESSVADNENDTDERVEQLQQQIEQEKKSALDLSESFDAKIQELQQQVQQEAGKVEQLEKEKQQSLEKIAQLEKEIEQEKQQNSELVAQIEKEKESSQDTESLLEKIKQLEAQVEEEKKQNSDLVGQIEKYSENQKDEENLLQHKVEELVNQVAEEKRINSDLTAQVSTIESLVSKISQLESEKISLEQSDELKAKIQELESQVEQEKKVAQDYEQEKQSQQQHLVEIKDKLEQLESQLNDEKKSHSELLDSKIQLENESNELKDQVEQLNIKLNEANAASSPVVEDQSSRVDELEKEVESFKSKLDDQVKLKVELQSQYEQERSSNQYLISQLEEKDQQMKENLAAIKTVKDLQNLVRSQQEKIALLEKHLQEKIEGGGVGGNASTGDEQEVNVNFVDHSDQEHDSNNLHLSQNEFQTFISNDDNENTINNNSTTQEQLIIPVVDNTNQESATTEHQQQQSERPKRVEDIVDALFQSGTPPLRSSSSFNQLVDLSLDDDDSNTQEEEQTDRPPAIAEDLGEMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V715</accession>
    <name>A0A8J4V715_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002302</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000062">
        <property type="protein sequence ID" value="KAF2076397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V715"/>
    <dbReference type="OrthoDB" id="1274115at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051911">
        <property type="entry name" value="SDR_oxidoreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43976">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43976:SF16">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="287" mass="31810" checksum="DB275E037E265B1B" modified="2022-05-25" version="1">MEPIITNNKNNNDKVFFIIGASSGIGLSVTKQFISAGYKIAATSRSKDNLLKVFNIHDESRFLALENDLLDDKKIKETIDQVYQHFGRIDVVFSNAGFGIAGVTEEISDENLRSLFDINFFASASVLRHVTEYLVKGSHVFMVSSFGGFHPIPSNSAYNATKFAMDGLSESYAEEVEDLGIKVTIINSGSFASSFGSGMKPAARDLKEKYSAIYKKTHELVNNLEYKDPTKFAEIVLEVTESASKAPLHLFIGPDANNFANIKIDRLVKELKENELLTTQRFLTPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V804</accession>
    <name>A0A8J4V804_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_004187</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000143">
        <property type="protein sequence ID" value="KAF2074519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V804"/>
    <dbReference type="OrthoDB" id="10259133at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030131">
        <property type="term" value="C:clathrin adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030665">
        <property type="term" value="C:clathrin-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09250">
        <property type="entry name" value="AP-1_Mu1_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14835">
        <property type="entry name" value="AP1_Mu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000006">
        <property type="entry name" value="AP-1 complex subunit mu-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018240">
        <property type="entry name" value="Clathrin_mu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005992">
        <property type="entry name" value="Clathrin_mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00314">
        <property type="entry name" value="CLATHRINADPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00990">
        <property type="entry name" value="CLAT_ADAPTOR_M_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00991">
        <property type="entry name" value="CLAT_ADAPTOR_M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="8">
        <location>
            <begin position="169"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2074519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="428" mass="48578" checksum="CCB426934B4C0ED5" modified="2022-05-25" version="1">MAASAIFLMDSKGKVLISRNYRGDVPMSVASKFVSKILEEEDLNLKPIIQEDGISYIYVKYNNLFLLATTERNANAATILLFLYKMIEVFNEYFKELEEESIRDNFVVIYELMDEMMDFGYPQSTEPKILQEYITQEGYKLERGAKGIMLPAAITGAVSWRKEGIKYNKNEVFLDVVESINLLVSANGTVLRSEIVGAVKMKSKLSGMPELRLGLNDKILFENSNKTGAPKGKGVELEDVKFHQCVRLSKFENDRTISFIPPDGEFELMSYRLNTTVKPLIWVECISDTHAHSRVEYMVKAKSQFKGKSIANNVEIIVPVPPDADTPKFRCTVGTCKYAPEKDAIIWTIKQFPGGGKEFLMRAHFGLPSISDEKPATKPPIMVKFEIPYYTVSGIQVRYLKIIEKSGYQALPWVRYVCLSGDYQFRTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8V8</accession>
    <name>A0A8J4V8V8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MACPF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_003230</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000099">
        <property type="protein sequence ID" value="KAF2075449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8V8"/>
    <dbReference type="OrthoDB" id="21110at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742">
        <property type="entry name" value="COMPLEMENT COMPONENT C6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742:SF8">
        <property type="entry name" value="FLOCCULATION PROTEIN FLO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00457">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0204">Cytolysis</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035188845" description="MACPF domain-containing protein" evidence="5">
        <location>
            <begin position="25"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="domain" description="MACPF" evidence="6">
        <location>
            <begin position="499"/>
            <end position="846"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1140" mass="126959" checksum="E637BD0C46CF6B34" modified="2022-05-25" version="1">MGTVIKLFVLVTLFICLFTTALEAESTKFYLSSKSNCQVNCGGDLQNAFRSFKDAIETISRSQFSSDYPPILYVNEGDYFGNDNKEITIKINIEIISLRGSLNTVIDCQRAGYGFKILDSQSFSMSGFTVRECNAGRGAAFMISNQMTSFEDMVFTHNSAREGAAVYSTASILNVLNSFSYSNEGGPSFFIKGATANFDKVAFSLNEKDLYCEDSLIESKDSTLGSICTQCTILDVDSFENLCGGEKVNKHCNGDGVCQKWVENYSNCPNDCPLDIYSCHNDGICYPYVETQICPDCAVTENPGWKLNSHTGSIQKVSNPSLINSGSSWEFIQYPEVYNFLAKYPAVVSGTLSSTVKPTKDGYYYFKLETENLNSIVFVKGQVLFDNFFPEKIHKLIDERKILMQQNVWANIDVMFMSDNNQQRDFSLKWRHESETAYSQIPGYYKKNYYLYSCGDGICNELDPLSCLIDCHGNIEKACPGQSPPAKLQDYYTNIHDTIGTLLSNQYIFSLPGINYISHGIDITTHETLPTPLFDLSYCDNTSFSLVQDPRRGIVYSIPSGIYAQVAAKCTMDTTTKTYATAEALAKEKSDGYEVNVEASVDGSYLFVSMSAEASFSHSETKTTASEMQKKMDGSISVTELHCETSKIHLIETKFHPKFIQDISKAFVEGDQAKSDELMQNVMKKYGTIYYQSATLGGKLEVISIVSNSFTSRKSSNEVEKSTEVTASLQLSGSVFGASASAGIEGSVSLDSTNSESEQNDYETNSMRSTVKVYGGEPGSYGKDNDANAFNSWAKNVDIVPFPIDYKVGYISDIIPKSWFFKGTHSIKEAWERNELLLYKDYFKSKKSKHIDQIVLSTLKKDDTVYSIKFVGQSAGITQAFNLEIVNLDNVATTYTNLPSIPIGTGAKPLLILNKYNDYKGVKSLKFTDPNTQAVLQPSAEFSLYNLFTSRAYEFVYQTDKYIQKTPEEPNTMILEISGFKPTAPSSILTGPSGEDGDFSEEMPMPSDGTAKRVLATIHGTTGKNFVILRLQEETVYGTHESAYTGEPIKLEYKENIGKILGIDFSFIVDHGHDESIKRFTFSFESLIIKRFCPESAECIPKKTLNSQNYINAYQLFSDGYVIGYTYGKPTWFPINPISP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJJ3</accession>
    <name>A0A8J4PJJ3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010476</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001137">
        <property type="protein sequence ID" value="KAF2068199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJJ3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="130" mass="15324" checksum="41546ACB1A75A2E0" modified="2022-05-25" version="1">MDKYYRCVFSNLYISTKIFTHVHQIQLNDYSLKYNDIVDAGWMYRNGHIELLKEKINNTNIKLYVCNKELFSLIANAETCLFIALFERTKNSALGDLDNKSAYDDFLSNIHNKEVLMYLHKEGHLRSFNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJR1</accession>
    <name>A0A8J4PJR1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009729</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000790">
        <property type="protein sequence ID" value="KAF2068950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJR1"/>
    <dbReference type="OrthoDB" id="26679at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="179"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="7"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="347" mass="39616" checksum="ADAC14DF5EF19552" modified="2022-05-25" version="1">MIHSNTSQDLEKDNARLQKENWELRNYYKVGCSNTDHNSLYVQLETSNNENDRLKQENKVIKQHLEKLSEENDNHNDKMDLLLNKIESLADVNDKLNTKIESVNQENMKSLNIYGKKMDLISDKMESLAKVIEKLNSKIENSKLNEQHLEINDKVSKTYKEIETCKQDLMFLIYKSKSKIIDNSSFKMINDWIDDGKTIGFELLYKASENEFSGPSFHSVCDDKGSTITLIETTDGCVFGGYNSQSWNDVPKWYGDNKCFIFTLVNKHGIKPTKYSPGTANKNYVGSGKEAGPIFGDGYDIGVSLDPKKCTYQSFPSTYIDTTGKGKSTLTPSREFNIKTIEIYKCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PK04</accession>
    <name>A0A8J4PK04_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010284</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001015">
        <property type="protein sequence ID" value="KAF2068392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PK04"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="435" mass="51212" checksum="3118A255E78DE187" modified="2022-05-25" version="1" fragment="single">RCLEWGSNELFVSLWNRFKAKDQIRVSETNNSISFCQDPVDKTRHSEYNYDEIVYIEGCLNIHIIDFLIGEGYIGIIKQTRILLYRPDHLLRIKYTRKEDFQLIEKFIKLEILNFHQVLVRCIKKGNIALFEWLYPLQGEYKFKMHELLQELLKHPSTRVLKLLKPEFSQIGWERLLSIKSLKLCFLKQILASIPHQQNPLPTKLLQLLVGKSSYAHAKHVLSNYRFESITPILSTLARRDNPAMVNLIYQYRDTAFINTPTQQDWDSLFRKCIRANSDRGTTANLEYLINHTDHILSCPIPKDMDSINPHTLSAEAFMLIGIGGYLDPAKHDKFLQHVLLDGQSRFPDFFFLIDKEVFSDNIYHFVQYIVDKSNTGHHEGPIKYCLYYLYNNQHRFTDAPSDKLEICREILNQPIPEYLSTHIKSYYFNNKDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKA9</accession>
    <name>A0A8J4PKA9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009954</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000864">
        <property type="protein sequence ID" value="KAF2068724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKA9"/>
    <dbReference type="OrthoDB" id="1158011at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23799">
        <property type="entry name" value="UBCc_UBE2J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000109">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 J2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBC core" evidence="1">
        <location>
            <begin position="15"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="168" mass="19053" checksum="3294F34461059889" modified="2022-05-25" version="1">MSSTPSTIEHPPTKECISRLRREFIEISKNPVENILVCPHPDNILEWHYVILGPANTVYESGIYYGQLIFKYNYPLSPPSIIMTTPSGRFETGKRLCLSISDFHPENWSPSWSSSSILLGLLSFMVDTDITLGSINTTNDEKRLLASKSADFNKKNDTFCKLFPYLVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKI5</accession>
    <name>A0A8J4PKI5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009143</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000644">
        <property type="protein sequence ID" value="KAF2069535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKI5"/>
    <dbReference type="OrthoDB" id="24485at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051904">
        <property type="entry name" value="UPF0746_actin_org"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32488">
        <property type="entry name" value="UPF0746 PROTEIN DDB_G0280785-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="539"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="543"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="580" mass="68890" checksum="8FAECB33316EFA22" modified="2022-05-25" version="1">MVRLIQQKNDAKESKNFYRTPILSSDLESAYENPFYEFAVVAKDRNKATNDNDKYFWSIFRNSFIKNHIWSFIHKPMIPFKRYKDIYSIKWIFKNAYSVQFLIDKVKRNEYLSDILYISIVLDQFKSINDIEFYTIFDQRYKRYLNDPVMLKVKQFLDSKLNNNDSDKDNNDNNNKKEEQPIIIINNLTNDQKEKDPSYLSRLSLEELLEDYKTFRKRHFNNFKRALESKHLELAQYVLENWLIQSEESLIPRNDYGFIHMGDTEIIKYVCQVNHQNRFDLKVGLGNIYLRDDFEEIMKLLEDSGVIDKLCEDHMNYFKTKRNLEYFIKNGYACDSNLLKSICLGNKMQFDCLPLLMDKIPFDHAGLEKLFYYRSMLPREHIIYKQFFKRYTMDEFKEKYPLLAQRYFRYQEMYKGLYQPDYQNMKTLKDLKVKLKRIGEIGDSSAILMLIRTHYSELLLNYVESMDPYSDNSTKPDYADDGDFYAETELSIEMLLFALIQTSNVPILKMIFQEFPQLLETIYQDQERKKMYTIARMGAGARKPSDTNERGVRGPGKSVAPTAAPTGPIISTFTSKGAFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKQ5</accession>
    <name>A0A8J4PKQ5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009018</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000618">
        <property type="protein sequence ID" value="KAF2069670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKQ5"/>
    <dbReference type="OrthoDB" id="6130531at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053331">
        <property type="entry name" value="EGF-like_comC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24032">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="480"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="484"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="503"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="599" mass="65819" checksum="E439CBECCE8C90F7" modified="2022-05-25" version="1" fragment="single">MNIICNDIDISYSFKSTILVNVDITQDVIKISNGKYSFQIKDSKGELSNTIVVYGIDYPTFIQSNFVIFIISKGFDAIDTSKIIVGADLESFVPITYINDTTSSFILPFTIAKTYTTNLYDSQSSPPTNVMSFFTFFSPTIDSFYFKNMNMYIHGHYYSTSSRIKLNSVQCDSIEAKGKGGAICRFEPYLFLTKTVDMVVGVFSISEPSFNRSIENEIKFQRTVLLQILSSPTSSRIDVSVLGPLYRAPHIFGHTLEPYPVLSYNASIVSFIPPTGAYCDYLYLGFTDTQGDRISNSLYLCPTPVIHSISPRPDSLLGGVVTVTGSFFNTTVGYLSKPSTTLNYIFKNGSLLDCPQSTDYDPISFIYTFKCYFPPGSGGFSFIAKNSIGHYAQIDYSYTLSITLLTSNIKYGTAGKITIHGEGFSNPNVFVGGSECLDVLVENGGRLLTCLFKADVLPTNNEKTLELVLKNDGAFAYQVFSYICPTICSSHGVCNIESIQCFCENGWSGYDCSTCNPSISMITSTKYGAPGTVTIVGNDFVNLNLVVTIGGSFCGKIVVSQDLTTITCLFQSDVSVPNDNTLLEVSVTIDHKFTTSKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL99</accession>
    <name>A0A8J4PL99_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000892">
        <property type="protein sequence ID" value="KAF2068644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL99"/>
    <dbReference type="OrthoDB" id="22869at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="89" mass="10241" checksum="7DF80F37C793D613" modified="2022-05-25" version="1">MLPEQEKEGQNAFRPIPEIDPLTKCYGTFIHLSNSMNIRLPERKNRILLQECVQDSMFCLPPTLKRGKNFDGKIENSNQTAGFQAYLEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLB8</accession>
    <name>A0A8J4PLB8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF985 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009644</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000766">
        <property type="protein sequence ID" value="KAF2069033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLB8"/>
    <dbReference type="OrthoDB" id="6614653at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06121">
        <property type="entry name" value="cupin_YML079wp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009327">
        <property type="entry name" value="Cupin_DUF985"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039935">
        <property type="entry name" value="YML079W-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33387:SF3">
        <property type="entry name" value="DUF985 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33387">
        <property type="entry name" value="RMLC-LIKE JELLY ROLL FOLD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06172">
        <property type="entry name" value="Cupin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF985" evidence="1">
        <location>
            <begin position="5"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="178" mass="20500" checksum="4E9ED62E5C914D22" modified="2022-05-25" version="1">MNTKEYWIEKLKLVPHFEGGYYRETIKSKELVKVGDKPEKKLFSCIYFLMDVSDSACFHSVKCDEMWYFHYGSCLNVHCLKPDGSYELVRLGNNPDNGEVFQSCIQSNNIFGAAVEKDSEFGFAIASIQVMPGFDMSDFKLYPRSKLLEMFTDQKTIINQMAQEDPSMNQEKPLPISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLT5</accession>
    <name>A0A8J4PLT5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA-specific adenosine deaminase 1</fullName>
            <ecNumber evidence="8">3.5.4.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">tRNA-specific adenosine-37 deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_009360</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Specifically deaminates adenosine-37 to inosine in tRNA-Ala.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>adenosine(37) in tRNA(Ala) + H2O + H(+) = inosine(37) in tRNA(Ala) + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:50968"/>
            <dbReference type="Rhea" id="RHEA-COMP:12855"/>
            <dbReference type="Rhea" id="RHEA-COMP:12856"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:82852"/>
            <dbReference type="EC" id="3.5.4.34"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>1D-myo-inositol hexakisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the ADAT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.34" evidence="8"/>
    <dbReference type="EMBL" id="AJWJ01000688">
        <property type="protein sequence ID" value="KAF2069323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLT5"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043829">
        <property type="term" value="F:tRNA-specific adenosine-37 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002466">
        <property type="entry name" value="A_deamin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46516">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46516:SF1">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02137">
        <property type="entry name" value="A_deamin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00552">
        <property type="entry name" value="ADEAMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50141">
        <property type="entry name" value="A_DEAMIN_EDITASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="A to I editase" evidence="13">
        <location>
            <begin position="73"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="181"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="336"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="211"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="341"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2069323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="516" mass="58793" checksum="207539FC4C772AFB" modified="2022-05-25" version="1">MNEKNTSYSKPWINNQQEFADNLTLFCHNYFEQKLSKKGKPSNVEWTVLACLVLVVDQQDSCSSSSSPFNVLSIGTGNRCLGKSLLSRNGDVVNDSHAEIICKRSFQRYCYREIKNLLENSSYKSLLFEIDNNNNQRGIGFRVKRKENVSLHFYVNQTPCGDCSIYPVTNENDYNALKKEMEKESNSNNNNNNNNSNNNDHNNNNNNDNDNDTRQTKDDRPLKKIKVVEDIQRTGAKSVKGEPEDRKLEGENYHQVGSLRIKPGRGDQTESMSCSDKIARWNVLGIQGSLLSHFMDPIYIDSITVGDLFNYNSIKRALIDRIDFKELYDNNSVEISDDNKNTTTTTTTSTTTTTENISSIDSNNNNTSIKPFHLNKDLSIMKTNNTFKFSKIKLEFDKSIGKPVSSGLAINYSYSTTPAMAVHEVTIAANGKKMGTNVKNFNQLSQRSSLCKASLFVSFKEILNHLEKNDFNQKLLSSNYYESKHSSECYFKAYDKLKQLRFGNWLTNDLSYEKFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM35</accession>
    <name>A0A8J4PM35_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009193</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000653">
        <property type="protein sequence ID" value="KAF2069483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM35"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="23" mass="2710" checksum="C16DF92ACFAF43B0" modified="2022-05-25" version="1" fragment="single">MAIVMAYLIGKLFISVERVRERD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM88</accession>
    <name>A0A8J4PM88_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_009119</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000639">
        <property type="protein sequence ID" value="KAF2069563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM88"/>
    <dbReference type="OrthoDB" id="17983at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006838">
        <property type="entry name" value="ADTRP_AIG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10989">
        <property type="entry name" value="ANDROGEN-INDUCED PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10989:SF16">
        <property type="entry name" value="AT02829P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04750">
        <property type="entry name" value="Far-17a_AIG1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="238"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="276"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="307"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2069563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="321" mass="36494" checksum="CD114E4B9898ABDE" modified="2022-05-25" version="1">MKLLVNLLLFIIHSTFLSLEIYGIIEFNKVFETFGLNGNTNKISMLTFLTFWGQLILTLFFSVSVLVDLIDLTKLFNRAGDSYFSILKLRDLLFRSVVFPIGSVITVMFWGIYAFDRELIFSRALDAIFPKYLNHIQHTLPGIVVVLETLIVNHQYHVQRNLIENNNTYIKNLKQENGTTTTKSSSIQYTPITSNLKQDVANLVSFILFYLGILVYTRYELGFWVYPVLEVIPTHSKVIFIFTSCGFAVTIYVIGRTINQARWGGQSVFIIAKKPPTNVNNSNNNSSRNNNSSRNNNNNNDKTKQPSQASNSSSASKIKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMM0</accession>
    <name>A0A8J4PMM0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008682</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000559">
        <property type="protein sequence ID" value="KAF2069999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMM0"/>
    <dbReference type="OrthoDB" id="21452at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="56"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="145"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="436" mass="50491" checksum="DA62833A10C035D4" modified="2022-05-25" version="1">MSQNNSSNSNNNSNNANNDKIDKRRQPYTLLDRNNEPDEEDDDYRVHTELDLLNVNSSGDNNINNNSIGSSSGGDDEDGNVILHNDQEIGQDNNIFPNNNNNKKSWFNRFNELVLKAKRSDFNPLSQTNNSDSNEEEEEEDVDESYYNYYNNNNNNNPNNSNSNSQQQQEQVPLYSIHSPLPWTVRSSIYNIDNNDNNNPTDTISSTTTTTTTNNNNNSYFPFPYYTSRQELNQQQTDFIKNNEIMVIKQPNNSLQVGFSSEKFKNYDKKTGEHDLNAFYQAFNQNDEYVARIANLHAQAQRDIQDGENDNYSTFSDYGYERLYFEVERKKKFLFTLITIEIFYYVLILASFSSLQWVMVFLFGGIANDVLGSYSLSKSNIRLLHIFLFAEVILMIANYILPISGLFIFRFIIFIVGFRLRHELVLLNSINNSRNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMU9</accession>
    <name>A0A8J4PMU9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008534</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000534">
        <property type="protein sequence ID" value="KAF2070149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMU9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="163" mass="19105" checksum="054A6F6556AAB5FE" modified="2022-05-25" version="1">MDGRDVKQQQQQDDQDVDIDDDDYYSVEDIDFDVVEQEQQDHDVTISEQQQQQVYTTPDTIVDQEVTGDDSYNYDDDDDDKEKDSDQEIEDSISSTEDEEKESSSKATTKDTTIPLLPDREKEELYSILSNHYFRPSIINRLAITNKISSERVRFMLRNNFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMY1</accession>
    <name>A0A8J4PMY1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_007806</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GOSR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000437">
        <property type="protein sequence ID" value="KAF2070870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMY1"/>
    <dbReference type="OrthoDB" id="422156at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005797">
        <property type="term" value="C:Golgi medial cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048219">
        <property type="term" value="P:inter-Golgi cisterna vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023601">
        <property type="entry name" value="Golgi_SNAP_su1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21094:SF2">
        <property type="entry name" value="GOLGI SNAP RECEPTOR COMPLEX MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21094">
        <property type="entry name" value="GOS-28 SNARE- RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="263"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="470"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="702"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="46"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="8"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="263"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="470"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="508"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="549"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="596"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="710"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="732"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="747"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="773"/>
            <end position="788"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2070870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="859" mass="98485" checksum="44F6AF050FCE9A95" modified="2022-05-25" version="1">MTSRWDQNTGSGSSSFPYSSLNSNSNSNNINNSGGSTSIGIPNDSNNSAAGSLENFKKERRNLESEIDEKLGELSTLNDKVQREDYNDDSIEYSKIKFSSLISELDNAFKNLSRCNEMLVNDPNFSSNRVHKEKLEDYLNEYRKFKKNLVQTFESVELLYGGRSGGYRKNDDTEIPMSNLLRENSNLQSGSYMQDSILGQARQVHEALENQRRLIRGAHGKVTNMPGLFHTIDGVTSKIKRFKQRNVVVLGLVIGLWSFQPHQQHNRQQQSRGSANQQQQHHQHQQHNTTSYLNSHSLKSSDAGVLVIGGINFDRISLGKRRAVKFTLANIEHKEFRVNNIFIQQPVITSDIHHQQHHPFQIMTQFKFPLLIAPNTDLEGYVSFCTTRPGKFDGLLSIITENGMQQISLHGEGDIPTSPSQIIYTGNQAPIIRNTPKKLLDYSDIDNSNHQSTVGIWSVASPLFFDDDPYNNTTIGNTSDSKQQQQQHSNNNEKPTTATNVKVSRQDNNNITPNNITPKQNNYHNPPNSKEKKVSFSPYISESEIQEISQDDDEDDEDDSHQHNISDTYINNNHQQQQQRHHQPIRVNKPQQQQQHTNTYNHKPQDTSKVSHTRSNTVNIENDNEYQRKIDSYRKSISLFKKNYDHNSTTTNVNNNIEDEFNKHLNLYDAKLTEISQKAKQLDKKKTTKNPPIIFDIKSHIDQSNSNHHNNNHHNKHQQQQHKPQHQYVKPNHNSHIQNITPIKPHQPSHHNNSKQHHQQSTAIPINVHDDPPPIQKHPQPQPQPPYKRPAAPVYQDHKQDIQENNSIQLTNEDLAMLQEMQPKPMATPAAWRHFLVPPTPIHDTTFYRANDTPFKSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN79</accession>
    <name>A0A8J4PN79_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_009420</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000704">
        <property type="protein sequence ID" value="KAF2069259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN79"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057709">
        <property type="entry name" value="DUF7949"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25820">
        <property type="entry name" value="DUF7949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035252356" description="EGF-like domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="5">
        <location>
            <begin position="427"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="4">
        <location>
            <begin position="653"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="3">
        <location>
            <begin position="803"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7949" evidence="6">
        <location>
            <begin position="1052"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1018"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1026"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2069259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1106" mass="124123" checksum="63D6EEB7C5A1BEA4" modified="2022-05-25" version="1" fragment="single">MYRFVFLLFCIFVLLPNVYSLQVVDLTRHEMTNQYANVNGTVNPTWKCRIYNQILIEDPAGITNATIVEASYFRVLRKNATAILYTFTIDLLPSTTQYVIKIFVNGDPVFKTHTINYICDHPPTPLDITILDVKPIYTLSHLTLSMYFLFKMNNIDADKLNGPLFEILSYDRQYTTTSQYVDKGIYCAVLAPTINTTSTNPIEIGIRYDNYENQLYYFNYTLTSPFPDYNNQIVSSNPNFMTLPLDTSSNLRYPTFSSFFEVEFTENKPLLSLFSYEQKTNPAVLVQGNYTHGTYYSKFAYTPNNTQITFTALLDPMINQTKTFNYIASDKPTVSAVVASTALPDVVSQQFQTNVTFKADVIAWSSMVTILPYPFGYSSGNSISYLRKNDMPFNNENFISGAYGIKTGVDNDIVSYDFISGIPTIVPDNQPPVIESIESYVCPDGNHYVIKLRITDNLSGFSSIYYYGDYTDIVSGDLNDGYYEFLRPTNDLSFMLLGMGLIYDQLNNLDVFTYRYGDVLTIDLQEFPYKILNFSNVVNIEFEMNEIDVSHSDVYNRLFIYTSNIDKTIHPGMMVPKYGDEPINLDDQSLTYSGSWDDSMGCYVIPFMVRKNFSPGFFEYVLKFGAYSIHYGSFFGSWFGEKAMLNIISKTTDILGPIVINLSVENRAVTIPNSVFVTLVWNLTIHDMYNGFSNGQVSITSSLDLVKYNTSFTAADLIEGDIYDGVYQFKVRVNPKCKSQTFYISYLYLEDNGTYYSLYREGGEFPTNTLSQFINPFLFAPDVTTASSVVVDCQNALVDTTPPTLTAFSFSPNFVDVFDSGELYNNSREVIFQMTASDDNGILLGSPPTVYLQDKRTRVVSQVSELVSSTDTEAVFRCNFTVPFGFGFKSDIYVSVFGIVDNQSNFKGYSISQLAAASFPYKLQVQPLLTHNVSVFSTINLYESGHISIRGRNFDPTDQLLIRFNNGTFATLSNSIFNTNSIIVFNNPSFIPIQDLTITIQKSNGKFSNSVNVQVKKTPHYGPPASSTTSSSQESSSSDEPPVTNKPQTCISDCGGIDHGVCTPAGCVCKSPWVGASCTSQVIIIDPVINSTTPSTNIKVPTKDKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP06</accession>
    <name>A0A8J4PP06_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MRH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_007176</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000367">
        <property type="protein sequence ID" value="KAF2071515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP06"/>
    <dbReference type="OrthoDB" id="23854at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23361:SF20">
        <property type="entry name" value="MRH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23361">
        <property type="entry name" value="MUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01404">
        <property type="entry name" value="CIMR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035224817" description="MRH domain-containing protein" evidence="4">
        <location>
            <begin position="21"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="5">
        <location>
            <begin position="31"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="5">
        <location>
            <begin position="175"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="5">
        <location>
            <begin position="502"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="5">
        <location>
            <begin position="674"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="5">
        <location>
            <begin position="859"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="5">
        <location>
            <begin position="1034"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="831"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="831"/>
            <end position="855"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2071515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1186" mass="130983" checksum="E105CDA12286ABAE" modified="2022-05-25" version="1">MFSKFIICFFLLTLFEGLLSSSSSSFNFVTSSCKFTFNNNSYDLSALTLTDGSSYRAGYSLLKFDFIFNICGSTSHCNNKYSKRGNSQACSMFPLATQVGDATTEKAEENSNGITLRYTGSDFLCSRSNELVFTCDESTDMRITQAHAHKCKYTVNIKSKFACPFTPTNPLPDPANCTFPGTTLDSYFDLSALMLKNNNSYNTYIGYIERGAYSYYNLFFSICGQIDACQDYKPVTDVKQYQSCQVYPSQNSSIQTGAPLKITSIPNDRGITLVYKASQEYSGCQRYIQLVLTCSRNNSFKIGKAKEISPCGFEIPIDSKYACPIYFSGGSSSDDIPSSSSSQDEGVNCILNNLDTVPYGFDLSPLALANNSVYYQAPFQVVDSAFTDSFTLLFSICGNVSVDQCRHYNQLSGNSTSYQSCQIYDNKYSIQTGTPSLLSFNTSALGLSLTFNSNMYSQSITRQNRINLFCKPNTTFKIISTSIETAPLTYQVNIDSRYACPSQCIFSDDKGEYFDLSPLILPNNSAYIAPFGYLDHGAFISKPLYFSICGQIDVCKQKTSPSKSTTFQSCILGPSTTTQIGSPINLETTLSHNRIVLKYTSDRYPCGSSFYSFNIIQLLCDNSTEFNIVSSYEAILCNYQVDIRTKYACSNKRDNSDNSNSYSSNDSTSQEIDSRCLIEGPEGTNSYYNLTPLALPNQSYYSATFIGHGTHAEDFTIYFSICGKVKICETNFIQLSGNSTSYQSCQSYIHNNLYAVKTGDTNGFSSSIESYGISLEYSSLNNYFSCIRVQIIKLICDSTTDFTILSTAEPSGCVYEIRAKSKYACPIYNSPSSESSISSKSSSSETISSSSSSSEPPIPSCIFQGNGDSYFDLSPLASKNNSYFNVSFHVNNGNDNFNVLLTICGPISTICQQMVEMTGHAIYQSCQLYTGMYAYQTGLPSLVTHTFSKNTITLRYNSNIPFSGCTRQNVLVFTCDPSETIKVMGSSVPQSCVYQVDFNSKYACAKVPSSSSSIDNNTTISVPDVSSESGPILDNCKFRGINGTYFDLSPLKTTTLGSFSVANLEGKGVKGQLFYNPCGQVDACRDVNLIQMTGDADSYQSCHVYQDKYSYQTGIPTRGSYIVSADNKRITLTYNSNVYYAGCTRSNVLIYHCDPNQENKLYKASERDCIFTLEFFSKHACAKSDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP45</accession>
    <name>A0A8J4PP45_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007110</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000361">
        <property type="protein sequence ID" value="KAF2071577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP45"/>
    <dbReference type="OrthoDB" id="15041at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035188796" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="30"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="159" mass="17946" checksum="BED73C13A5BE21C8" modified="2022-05-25" version="1">MFWCSRTKVGLSSFLLFLFGITQVVLTWANKDVDTQHTWAFFANGCVCVLTGFCGILSTILEKKTIKKIYFLMCCYTILESIIVFIIYSSLLHKYIKNACGGDEFFNAYCQGIKIYLSTSTAVFWAFIVLLVPYSTIVSYGHLAGNDKTGTIEMDSRNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPB2</accession>
    <name>A0A8J4PPB2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3-hydroxyisobutyrate dehydrogenase</fullName>
            <ecNumber evidence="4">1.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_007363</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>3-hydroxy-2-methylpropanoate + NAD(+) = 2-methyl-3-oxopropanoate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17681"/>
            <dbReference type="ChEBI" id="CHEBI:11805"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57700"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-valine degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HIBADH-related family. 3-hydroxyisobutyrate dehydrogenase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.31" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000388">
        <property type="protein sequence ID" value="KAF2071313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPB2"/>
    <dbReference type="OrthoDB" id="435038at2759"/>
    <dbReference type="UniPathway" id="UPA00362"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008442">
        <property type="term" value="F:3-hydroxyisobutyrate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000006">
        <property type="entry name" value="3-hydroxyisobutyrate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000119">
        <property type="entry name" value="3-hydroxyisobutyrate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011548">
        <property type="entry name" value="HIBADH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029154">
        <property type="entry name" value="HIBADH-like_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015815">
        <property type="entry name" value="HIBADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01692">
        <property type="entry name" value="HIBADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22981:SF7">
        <property type="entry name" value="3-HYDROXYISOBUTYRATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22981">
        <property type="entry name" value="3-HYDROXYISOBUTYRATE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14833">
        <property type="entry name" value="NAD_binding_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000103">
        <property type="entry name" value="HIBADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0101">Branched-chain amino acid catabolism</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase NADP-binding" evidence="12">
        <location>
            <begin position="25"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyisobutyrate dehydrogenase-like NAD-binding" evidence="13">
        <location>
            <begin position="186"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000103-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF14833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2071313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="323" mass="34044" checksum="ECEDAC2F361C04DD" modified="2022-05-25" version="1">MLSNRFNQLMFNNIRYYSTSGKPLIGFIGLGNMGGHQAMNLIKKGHNLVVFDVSKDNVNRLADKGAKVAQSPAEVAKQADVIVTMLPASAHVKSVYCGENGILSTIKPGTLLLDSSTIDPATAREVADKAAQHKATMLDCPVSGGVGGAEAGTLTFMVGGSAGDFEKAKPYLECMGKNIVHCGDVGTGQVAKVCNNLVLGISMIGVSEAMNLGVKLGMDPKKLAGIFNTSSARCWSSELYNPCPGVLENSAATRGYTGGFGSALMNKDLGLAVDSAKSIGEPLFLGNSAHQLYTLVCAKGEGHRDFSVVYEYLQKNNQDNNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPM9</accession>
    <name>A0A8J4PPM9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Carboxypeptidase</fullName>
            <ecNumber evidence="7">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_008189</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="7"/>
    <dbReference type="EMBL" id="AJWJ01000484">
        <property type="protein sequence ID" value="KAF2070491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPM9"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF472">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE CPVL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035342792" description="Carboxypeptidase" evidence="7">
        <location>
            <begin position="27"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2070491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="496" mass="56027" checksum="9684DA541596C22D" modified="2022-05-25" version="1">MRQTSRVFLLLVLGVLFISQIPSISARRENSHPFFKKTKFDPIPHHVKLESELDAGETLYASKYVDDHKTAKKLSCVEIGAPFTNCKKLESIDYSLDATTDPFSFSGFITVNETDNSNLFYWFFEAQNGNKDAPVVLFLQGGPGASSLFGLFSENGPYAMLPNLTMVPRNPSWNTNYSMIYIDNPVGTGFSFTEGALNSNEDEIASNLFSCLQQFFKLYPEYADNEFYVAGESYGSRYISALAYYTIQQNYAGVNLKGIAIGDGFADLRSMVTQYANLCFYTGLCDMVQTGIFEMYQAKIIAAIDQEQWLIANDLFTDLVNGPPDLFTNYTGSSNYYDIRTSSEPSYGGDFETFVNSTQIRQLLHVGNHYFQDNDDVYNALREDIPKSSTPLYPPILSSGMKVMLYNGNMDFIVHPTLTEYFITTIPWSGIPFLYESPRIIWRLPSDPSSVAGYVKQYQNFIQVTVRDAGHMVPFNQPERALDMITRFIENLPFPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPW9</accession>
    <name>A0A8J4PPW9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007969</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000455">
        <property type="protein sequence ID" value="KAF2070715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPW9"/>
    <dbReference type="OrthoDB" id="18636at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="237" mass="26231" checksum="D0AABAC3FFC783A5" modified="2022-05-25" version="1">MNNFMLKFVASTIYRVVGFRRIASMTALSIGPKLVDHAVNKVSGGLLPVSVVRLLRTSLSYAPVAVVMGTGGTPIASALTYYILVQISDTLCMLLIREATGATFRGVKYVVKSTYDSITGNAAREEQELCQELETWELLEKEEISTDDLPPEKLKEIEQYLDSVNLDELKIDIDTTSNNNNSNSDGDNNNNNESKNIHLNSSQAALSTISNPQEIENLLKEQLSLNKELEELEFVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ01</accession>
    <name>A0A8J4PQ01_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_006845</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000333">
        <property type="protein sequence ID" value="KAF2071853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ01"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="3">
        <location>
            <begin position="8"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="216"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2071853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="293" mass="33983" checksum="81A8D09E96D05CD3" modified="2022-05-25" version="1">MENLKTITVLLIGKKNSGKSTLGNEILNSEYFLESHLDFKENIKKEHQKEEIIIDQVRYVIVEVNGLGNPELGDQDMINIFSNACYQVPNGFNQIFYTTSEIIDETEEKHFNCISNFIFGSTDLFQHITLVRTKFPDFENRKHCEADLELCLGKSNLCFWNFIKGSNKVIHVNTPSIYQDDFNENITGPLLRSHLLENCQDIFKPKELDDFNGLLIKNQTNKIDEIQNQLKELKEKLLCLTLEIKQEGEKHFQLKQENQKLQELNDELSSSIQNKLDLTLDLNLNQFLDIPPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQR0</accession>
    <name>A0A8J4PQR0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Transaldolase</fullName>
            <ecNumber evidence="4 8">2.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_008199</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the rate-limiting step of the non-oxidative phase in the pentose phosphate pathway. Catalyzes the reversible conversion of sedheptulose-7-phosphate and D-glyceraldehyde 3-phosphate into erythrose-4-phosphate and beta-D-fructose 6-phosphate.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-erythrose 4-phosphate + beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:17053"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Carbohydrate degradation; pentose phosphate pathway; D-glyceraldehyde 3-phosphate and beta-D-fructose 6-phosphate from D-ribose 5-phosphate and D-xylulose 5-phosphate (non-oxidative stage): step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the transaldolase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.2" evidence="4 8"/>
    <dbReference type="EMBL" id="AJWJ01000486">
        <property type="protein sequence ID" value="KAF2070474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQR0"/>
    <dbReference type="OrthoDB" id="2015515at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00414"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004801">
        <property type="term" value="F:transaldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00957">
        <property type="entry name" value="Transaldolase_TalAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000131">
        <property type="entry name" value="Transaldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001585">
        <property type="entry name" value="TAL/FSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004730">
        <property type="entry name" value="Transaldolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018225">
        <property type="entry name" value="Transaldolase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00874">
        <property type="entry name" value="talAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683">
        <property type="entry name" value="TRANSALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683:SF18">
        <property type="entry name" value="TRANSALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00923">
        <property type="entry name" value="TAL_FSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01054">
        <property type="entry name" value="TRANSALDOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00958">
        <property type="entry name" value="TRANSALDOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0570">Pentose shunt</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0704">Schiff base</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2070474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="321" mass="35699" checksum="6BCD02E2B39F22B6" modified="2022-05-25" version="1">MANALEKLKEFTTIVADTADFEVLSKFGSQDSTTNPSLVVAAAGNPKYKALIDNAIEYAKKNASDKKAQLSLAIDKLFVNFGVEILKIVPGRVSTEVDARLSFDTQGNIDKARELIKLYKEAGIDKERVLIKLASTWEGIQAARVLEVEGIHCNMTLLFSLVQAQACADANVTLISPFVGRITDYFKQKDNVKDYVPSQDPGVLSVQAIYDYYKKHNYKTSVMGASFRTKEQCIELTGCDLLTISPALLEQLQKADASLVSKKLDASHPITSTIPDKVIPDQKLFNWELNKNEMAHFKTAEGIRKFTEDLEKLEKTIEKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQR7</accession>
    <name>A0A8J4PQR7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">VLRF1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_006604</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The VLRF1 domain mediates binding to the 60S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the ANKZF1/VMS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000312">
        <property type="protein sequence ID" value="KAF2072084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQR7"/>
    <dbReference type="OrthoDB" id="429841at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047139">
        <property type="entry name" value="ANKZ1/VMS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041540">
        <property type="entry name" value="VATC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041175">
        <property type="entry name" value="VLRF1/Vms1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16036">
        <property type="entry name" value="ANKYRIN REPEAT AND ZINC FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16036:SF2">
        <property type="entry name" value="TRNA ENDONUCLEASE ANKZF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18826">
        <property type="entry name" value="bVLRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18716">
        <property type="entry name" value="VATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52044">
        <property type="entry name" value="VLRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 14" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 14" id="KW-0540">Nuclease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="VLRF1" evidence="16">
        <location>
            <begin position="245"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="13">
        <location>
            <begin position="534"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="35"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="118"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="283"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="404"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="605"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="139"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="288"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="609"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="626"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS52044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2072084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="743" mass="85820" checksum="5D7CD26228F23717" modified="2022-05-25" version="1">MKRVSVFQLSNSTLFQSGGDGQDNLELVLNAITKPSTVSPSTTPQKQSQQQDTTTTSTYNSTESEFEHTCNACLQKFTNKELRNQHYRSGIHRYNLNLKLSHMAPVTEKEYANLIEDFKSKREDSDDDYSEESSSSDGGESDDSQDSSDEEALLKKEYAMHSSNQYDEELYYNTQGYQFYDKSMEVNDPKLKIISREKKLQFAVWKCLLAPPSTYRYLSMASDAKTVEKYTDQITNNFKKICRSPDMKWLVLLCSGGRFAGAVYTAPTGKCVDHKTFHRYTIRKKQGGAQSTKDNQSGNKKSAGAGIRRYNEKRLREEVVEQLALWKPHVQEASHIFVFAPKGNTRDILFPADGYISPDDERIRTIPFPIIRPTYAETQRVSTWILSVDIELFDEEKAQVERQERERLAQETQDKLDAEKKQKEKDLRRKEYNEREDVVLAKQQKQQQQQEQQEKDRLALYENDKLFVAARNRDLATVQRLLDDDLDYELPEPSINDDLITPLFIAVDNKDMAMAAYLLQTLPGDVNLLNPKWDYKTCLHKASADGNIEMIKLLLNNHADPTITNQLRSETPYDCSVNQKTRDFYREWAGDNLDAHDYQKAHIVPLTKQMKEDKEEKKKLKRKQQRDREKLKKAQEKEEKEKEAVKNVALDKLRQMKIGLEEERLAQQQSKLSQQSKLSERELRAIAAERRFSGSVGGGDDISKKNNCCQFCQKIIVGTPFERLNFKYCSTTCTASHKKELQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR36</accession>
    <name>A0A8J4PR36_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_006375</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="AJWJ01000292">
        <property type="protein sequence ID" value="KAF2072297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR36"/>
    <dbReference type="OrthoDB" id="1668230at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE GREATWALL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="54"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2072297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="293" mass="33469" checksum="38C828163585CAB4" modified="2022-05-25" version="1">MGTILNPTREQHQRGDGEVLKSIGVPIYDSRLLSINNNISNISTLNRVINCLRYKQYVRIGGGGQGSVFMLNDGSTPLAIKICGQDQLNLLELQKDALVDSPYLSRVSFTFCNEGKQIMVMDYLEGKDLRWYMNNWPSMLKSRVKTGMAQIGYGLYLLNFNRIAHCDIKMDNVIDVNDKWIVIDFGHCHQIGPWNRVGTHCYMLESDVSITRDSYGWGCIFYEIYQGEPPYSNRKTELEFDKIPDPLLGDLLSKLLHIDKDKRITIEEALAHPYFHDIDLESIKKGDIVPPCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRA4</accession>
    <name>A0A8J4PRA4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006327</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000287">
        <property type="protein sequence ID" value="KAF2072357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRA4"/>
    <dbReference type="OrthoDB" id="6699954at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03710">
        <property type="entry name" value="BipA_TypA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03691">
        <property type="entry name" value="BipA_TypA_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01891">
        <property type="entry name" value="TypA_BipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.250:FF:000001">
        <property type="entry name" value="GTP-binding protein TypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000002">
        <property type="entry name" value="GTP-binding protein TypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000003">
        <property type="entry name" value="GTP-binding protein TypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000055">
        <property type="entry name" value="GTP-binding protein TypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.250">
        <property type="entry name" value="bipa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006298">
        <property type="entry name" value="BipA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048876">
        <property type="entry name" value="BipA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047041">
        <property type="entry name" value="BipA_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047042">
        <property type="entry name" value="BipA_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035651">
        <property type="entry name" value="BipA_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042116">
        <property type="entry name" value="TypA/BipA_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01394">
        <property type="entry name" value="TypA_BipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF8">
        <property type="entry name" value="TR-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21018">
        <property type="entry name" value="BipA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="1">
        <location>
            <begin position="116"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="717" mass="78895" checksum="AB4B17132204F463" modified="2022-05-25" version="1">MIGLNASKRMMSSVTKNASASIKSFSNIGKVSIASKSLVASTTSTSTITATTTTNSNKININNVINNKNIKNIAKIQIKTNNGQMNMSSNVINRLTDSKLGSMDIHIYRSYSTDHKIIRNVAIIAHVDHGKTSLVDQLLTQSGAIKQTEKDERVMDSNALERERGITIMAKCTSFEFNNHKLNIVDTPGHGDFGGEVERVLSMVDGVVLLVDATEGPMAQTKFVLSKALTAGLKPIVVINKMDRSTIRVDEVENEIFDLFATLGATDEQLNYPTLYASGRQGWAIKNRDDEQKNIHPLLDTIIDYVPHPNVQPEEPFSMLVTNLESDPFVGRIVTGKVYSGKVKIGTPLRCLGINGEVIEDGKVTKIFARKGVERVILEEGDAGDIIGIAGFKNATVTNSILDPLIKTPIPANPIDPPVISMHFEANNSPFAGKEGTQLTSLKIKQRLLKELESNVSLNVTFTGESFEVKGRGELQLGILLENMRREGFEIAVSQPRVVFKRENGELLEPQEEVTIDVDTEYSGHIIEKLSKRKGDILEMKQSMGKSRLVFLCPSRGLIGLRSEIINDTKGTGVMNHLFHSYVPHKGPMQNMEKGALISMAEGVTTSHALSSIEDRGTLFIPAGVQVYSGMIIGENSKTNDIDVNPVKSKHLTNIRTVSKEEGVRLAAPKVLKLEEAISSVKEDELIEITPQNIRLRKKELDITKRQKISRDKKHIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRU9</accession>
    <name>A0A8J4PRU9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Dipeptidyl peptidase 3</fullName>
            <ecNumber evidence="4 14">3.4.14.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Dipeptidyl aminopeptidase III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Dipeptidyl peptidase III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_006462</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>Release of an N-terminal dipeptide from a peptide comprising four or more residues, with broad specificity. Also acts on dipeptidyl 2-naphthylamides.</text>
            <dbReference type="EC" id="3.4.14.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14 16">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14 16">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the peptidase M49 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.14.4" evidence="4 14"/>
    <dbReference type="EMBL" id="AJWJ01000299">
        <property type="protein sequence ID" value="KAF2072217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRU9"/>
    <dbReference type="OrthoDB" id="4694525at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.30:FF:000001">
        <property type="entry name" value="Dipeptidyl peptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.30:FF:000002">
        <property type="entry name" value="Dipeptidyl peptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.30:FF:000003">
        <property type="entry name" value="Dipeptidyl peptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.30">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005317">
        <property type="entry name" value="Dipeptidyl-peptase3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039461">
        <property type="entry name" value="Peptidase_M49"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23422:SF11">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23422">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE III-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03571">
        <property type="entry name" value="Peptidase_M49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007828">
        <property type="entry name" value="Dipeptidyl-peptidase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6 14" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="7 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 14 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 14" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="8 14" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 14 16" id="KW-0862">Zinc</keyword>
    <feature type="active site" evidence="15">
        <location>
            <position position="435"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="434"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="494"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR007828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR007828-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR007828-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2072217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="693" mass="78160" checksum="DEED91AE1D90D51F" modified="2022-05-25" version="1">MTDQKTIDLHTTPIEIPIYRLNVTEPFKSLTEKEKLYAHHFSQACWWGSKVCLGQTSEESPVIFALLQQLFSIDQVPELKSKLVPSIISLEDFNAILYYSCLFYSNMGNYLSFGDSKFIPRINKDKFQLVINSLNDSKLTLLWNKCSELMYSLKKNELELGIDGTGVSTYYNPGITKDEIKKVQDYMNSVSLSAYNTRLFKVGQDHYKLLLASAETKPTTTVDYQGTKIDITYGDWSASLAKVAHHLEQALPYAANENQSQMIKKYVDHFKLGDINDHMESQKYWIKDVGPVVETNIGFIESYRDPFGVRGEFEGFVAIVNKELTAKLGKLIDNAETFIAQLPWNKTTKAFEREKFIRPDFTSLEVLTFASTGVPLGINIPNYDSFKEIGFKNVSLGNVLAARKKQKENFLRDEDQDLYSELFSHAFEVQVALHELLGHGSGKLVQVDANGKLNIDPETINVVTGKKIDPQTEAYKVGETYDSKFENVGSMIEENRAEAVALSLSLSPAILSLFGYTGQKAEDITYIMWLIMAKAGLSALEFYSPPTSEVPEGRFRQAHMGARYALFNVFLKSKIISLNVTEDDVIVTLDRSKIKTVGIDAVNDFLTKVNVYKATGNVTAARALFKEYQPVDQEFLRIRDIVLAKKKPRAVLVQSHTYIKDGQVILEDFSDNCEGLIQSMQKRFGTDNSDLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRY4</accession>
    <name>A0A8J4PRY4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Acetyl-CoA hydrolase</fullName>
            <ecNumber evidence="4">3.1.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Acetyl-CoA deacylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_006779</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + H2O = acetate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20289"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="3.1.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyl-CoA hydrolase/transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.1" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000328">
        <property type="protein sequence ID" value="KAF2071900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRY4"/>
    <dbReference type="OrthoDB" id="10250396at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008775">
        <property type="term" value="F:acetate CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003986">
        <property type="term" value="F:acetyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006083">
        <property type="term" value="P:acetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.750.70:FF:000002">
        <property type="entry name" value="Acetyl-CoA hydrolase Ach1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1080.10:FF:000003">
        <property type="entry name" value="Acetyl-coA hydrolase Ach1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1080.20:FF:000001">
        <property type="entry name" value="Acetyl-CoA hydrolase Ach1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.70">
        <property type="entry name" value="4-hydroxybutyrate coenzyme like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.20">
        <property type="entry name" value="Acetyl-CoA hydrolase/transferase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.10">
        <property type="entry name" value="Glutaconate Coenzyme A-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026888">
        <property type="entry name" value="AcetylCoA_hyd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038460">
        <property type="entry name" value="AcetylCoA_hyd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046433">
        <property type="entry name" value="ActCoA_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003702">
        <property type="entry name" value="ActCoA_hydro_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43609">
        <property type="entry name" value="ACETYL-COA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43609:SF1">
        <property type="entry name" value="ACETYL-COA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13336">
        <property type="entry name" value="AcetylCoA_hyd_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02550">
        <property type="entry name" value="AcetylCoA_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-CoA hydrolase/transferase N-terminal" evidence="9">
        <location>
            <begin position="24"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Acetyl-CoA hydrolase/transferase C-terminal" evidence="10">
        <location>
            <begin position="341"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2071900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="532" mass="59279" checksum="67BF737BA63E03AE" modified="2022-05-25" version="1">MHKVVEHGTKSLITSRIKRPSLLSKVITDPNHLIPYFQNGQYIGWSGFAGTNYPKVTPIALADHVEKNSLQSKLKFNLFVGASTGPETEGRWAALDMIERRYPHQSDSSVRKGINTGNIKFADEHLSIYANDLLYGYYTLKKEKGDKRKLDIAIIEATEITEEGYIIPGASVGITPEILQMADKVIIEINTSLPSFKGVHDLVKHDPPPFTKPYQLTRVDDRIGVEAFPIDPEKILAIVESQLPDNTSDPAPEDEKSNAIAHHIVEFFKAEIEAGRMPKNLHPIQSGIGSIANAVIGGLSRGPFENINVWTEVIQDTFLDFFDNGKLNFASATSLRFSPSGFNRLFNNWDNYVNKMVLRNQAISNSAELIRRLGVIALNTPVEFDIYGHINSTCVDGSKMLNGIGGSGEFLRNSKLSIVHAPSTRPTKSDPHGISTVVPFASHIDHSEHDVDIYVTEQGLADVRGLDPIERAKVIIEKCAHPIYKPILKEYLETSTQICLKKGAAHEPHQLDKAFKFYTNLVEKGTMRFDKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSA0</accession>
    <name>A0A8J4PSA0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005811</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000242">
        <property type="protein sequence ID" value="KAF2072863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSA0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="505" mass="58212" checksum="D92151B9F517E98F" modified="2022-05-25" version="1">MTSITTTDRFYSLWRNRYIRDCIRCNVWRNRVINTTLEYLDDNYRYLSLFAFNDRIEYNIAIHLKINKRDTLIAYVNNKHKELIDSIFIDYDIDVTLLPPLDHTPLQTNQQTVLQQQQQRELLQQSKVLRFDCSLFHQGLRSLSFFILNQHTVGSGKPLPDSITKLNIYSRFRFEMGNIVVDSILAHLPSRLRSLTLPNGLVINTPTCAIPDTLEYFNYGSTHENLKGFVVPPGKRFGKCVLKIKTNQDLEWVTQHTWVHKVSFLIGFEPTAHTLASHITHIDFEFIDGKLEKGMLPISLINLSIYQYDEPLEVGVLPSGLQDLMLETYDYPLEQGHLPDSLTALCLGSYNLPIKPHVLPNNLTRLTMDSFDQEIQANTLPRSLKYLDISVFDESFEHVGPLDNLIELTVDDLHESVATLLQNVRTIEIRTNQISGNVYLKDTAIQNLDIHSANRFSLYPGFLPSSVKKIIMKGFDIKMDNLIPQSCVYLKTDIVEHQTDSFVKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSB0</accession>
    <name>A0A8J4PSB0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zinc finger protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_005802</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000241">
        <property type="protein sequence ID" value="KAF2072878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSB0"/>
    <dbReference type="OrthoDB" id="25761at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034247">
        <property type="term" value="P:snoRNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16539">
        <property type="entry name" value="RING-HC_RNF113A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039971">
        <property type="entry name" value="CWC24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12930:SF0">
        <property type="entry name" value="RING FINGER PROTEIN 113B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12930">
        <property type="entry name" value="ZINC FINGER PROTEIN 183"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="199"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="268"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="199"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="50"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="55"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="137"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2072878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="334" mass="37969" checksum="BA6DDDD444B5AD2C" modified="2022-05-25" version="1">MTNNENLDTNKIDSIDSNNTTNTIESNNTTAPTAPVCVFKKLPKVRNIRKRDTDTIIQDNTPTAATTTKEEDQNEQDNTEEQQNKKLKTKINQYSTKDQNKKKEFSFEASGTAKSYITEAEVTSTEFLKEGDDDDLYTNRKSGDKDKDTSVNNDDGLYRGMKSYNNYVEKKSDLTYKGTGVKAGPIRNATTVRLSSRIDYQPDVCKDYKQTGQCTFGDACKFLHDRSDYKSGWQIDKEYEEEQKRKASGGEPITITTDKKEEELPFACFICKKEYDNPVMTKCKHFFCEQCALDHNAKSKKCFICGEQTQGAFSQPKKIIDKLVAKSRAHFSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSB5</accession>
    <name>A0A8J4PSB5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007126</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000363">
        <property type="protein sequence ID" value="KAF2071554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSB5"/>
    <dbReference type="OrthoDB" id="20988at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="25"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="364"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="133"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="245"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="25"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="382"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="404" mass="45884" checksum="E7FB13A6D4C583D3" modified="2022-05-25" version="1">MYTMPSFFKPSSGLKDVISLYGNTNNNNNSNNSSTPAATDATPAATTPQITTPIKKKQETIINKTPTKTLNERHSSSSEDDDEEEESKTLRVLSSSPNSPNFKSPKNKTSSLKQKRFKNIVNQLPAIKSMDDVVKLQEEITENRDKISMLQQQLNTVQKEKSNIETEKQTLLQQLKDLENKIETTQAREKETLEKLEEMEILNNKTIESLHQEFDSKMSRIRVEKTISDNLKHQEIVSLKDQLSLLATQNQLSKQQLEKAQLENDEKANTIKTKTNLIESLESDIQQSNQSNQEMNITLNSTNETLKNVRKELFYSSCLAIKLDMVMSNKPCNISTQDLWEVAESQQITPANFAKWITQKLMEYQNNMPPPSPMKQISTKNTTTTTTTTTTSTGKSPKRNSKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSD6</accession>
    <name>A0A8J4PSD6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Short-chain dehydrogenase/reductase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_010069</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000910">
        <property type="protein sequence ID" value="KAF2068606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSD6"/>
    <dbReference type="OrthoDB" id="13950at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05374">
        <property type="entry name" value="17beta-HSD-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051911">
        <property type="entry name" value="SDR_oxidoreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43976">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43976:SF16">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2068606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="275" mass="30591" checksum="81CA618E5E887DD5" modified="2022-05-25" version="1">MSSQLVWFVTGISSGIGKELVEKLLVAGQKVAGTTRKSSSINIPENPNFLLIECDLKTEAGIVSALAATVQKFGRLDVVVNNAAFVTFGTNEEISDKELRDLMEINFFAPMTVNRVALGYLRPQRSGLIYNISSMAGFTPVKCWGTYNAAKFALSGVTESLSQEVSEFGVRVCSVNPGFFRTPIHDTHPHSEKTIEEYNSKKFILDLYKDRDTRPFGSTAKLCDLMIELALDPNSKIPLQLFTGVDAYEWVKARMDRMVKDMSDNEHRTTTTLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSF4</accession>
    <name>A0A8J4PSF4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Isopropylmalate dehydrogenase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_006271</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000282">
        <property type="protein sequence ID" value="KAF2072414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSF4"/>
    <dbReference type="OrthoDB" id="10261637at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000003">
        <property type="entry name" value="Isocitrate dehydrogenase [NAD] subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004434">
        <property type="entry name" value="Isocitrate_DH_NAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00175">
        <property type="entry name" value="mito_nad_idh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF34">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="9">
        <location>
            <begin position="25"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2072414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="349" mass="37359" checksum="75CE625738C4A8D0" modified="2022-05-25" version="1">MFSFNSLNRFAKVANRYYSSQNNITVTLIPGDGIGPEIADSVKKIFSAAKAPISWETVNVDAKTGVSTEIIDSIRKNKVGLKGPLGTPIGTGHQSLNLALRKAFNLYANVRPCLSIPGFKTRYDDVNTVVIRENTEGEYSGIENMPVKGVAQSIKIITKEASARVANYAFQYAMANGRKKVTCVHKANIMKQSDGLFVTTCREVSTRYPSIKYEEMVVDNNCMQLVLNPGKLDVMVLPNLYGDIVSDLCAGLIGGLGLTPSGNIGENGTAIFEAVHGTAPDIAGQGKANPTALLLSGIMMLRHLGLNSHADKIEKAVLTTISEGKSITKDLGGNSGTAEYTDAVIAKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSL9</accession>
    <name>A0A8J4PSL9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidase M66 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_006226</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000277">
        <property type="protein sequence ID" value="KAF2072471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSL9"/>
    <dbReference type="OrthoDB" id="19788at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051256">
        <property type="entry name" value="Dictomallein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019503">
        <property type="entry name" value="Peptidase_M66_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39540:SF1">
        <property type="entry name" value="DICTOMALLEIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10462">
        <property type="entry name" value="Peptidase_M66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51694">
        <property type="entry name" value="PEPTIDASE_M66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035243665" description="Peptidase M66 domain-containing protein" evidence="9">
        <location>
            <begin position="19"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M66" evidence="10">
        <location>
            <begin position="139"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2072471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="625" mass="69572" checksum="289ACCF03C5D4264" modified="2022-05-25" version="1">MYKYILLVILVSLSFTLGKKLEIKKLEAAQTNIIPAETGLSWTVENQTLHLVGNRETLLIIEFGLVNLTNAKVHGMDASNKSLGSLALLDPSQLPKTESNGTKYSLTAYSVLLPANWLVINAQVFFTADTFEPSDKKTLLVGCDSELDMYNLPFYLFGANESTIPFSKVSTISDDVRDELYNKWPISTLKFSNHAAKKIMWSTLVLSPNNTGPAVIARNKNDQRDGYEIMGIILMLLREIRSVNGESGTNNQIYAPIIWLNSKGKQEPAYGGLGGGNVGTGDPEYKGIYFHETGHSMSLPHAGENYPDHYPYEKGSLKGSTLGYDKTHNEFLSVLLPTESSVYKNCAKSRVLDANGKCFKQSAMQGGSGDQSKGYFFTMYADFECGKVQRYFEGITKIDNGQHVYDGGKIFKNSSMPSGYSRWDTIDKKYVEVKYITQQKGLYGFNNNLPIKRDIMVYTMIATVSLANTTGATQLYPPLYYKGGMIEYYDPTNATKLKEIIPNTSKFPWVCHASGCDWTIRAIYNDNSVDHYLLQKGLRSWFKPMGDISQDFFNPLKSSSLQTFVHNIPASKGVPSRYQILYTPLGFNGLPSNPTVMLDYSCSGQGESISCSPFSSQKTTKTKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSM9</accession>
    <name>A0A8J4PSM9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009947</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000863">
        <property type="protein sequence ID" value="KAF2068726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSM9"/>
    <dbReference type="OrthoDB" id="18358at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF29">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="119" mass="13078" checksum="19BE92B95A4B2971" modified="2022-05-25" version="1" fragment="single">KRGVIDGRVASVTNNLLPNYNGESNQFNNVQSYIGIGIRSYRKLVQLDPDFSVLIDQRPAEDSNTSVCSSSPKSKISKAQLAGIIIGSVAFAAIIVTMTAYALYKRKERVTLENKLRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU57</accession>
    <name>A0A8J4PU57_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000201">
        <property type="protein sequence ID" value="KAF2073491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU57"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="149" mass="17640" checksum="58B96101E3ED9FCB" modified="2022-05-25" version="1">MNTYRKSSSNNNNNFKANYETYFNISNIEHLNRSTTSSDDKVLFNLLNDTTASNNGLNSHQINNEKDFIYEDYDDINILKENIKQSIQFQQEIDVINKDLLKEKEDVQDFKMKMIMMNSKIKFHTLELNLDNYDDNPIIFNQTIAEHKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU91</accession>
    <name>A0A8J4PU91_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003761</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000122">
        <property type="protein sequence ID" value="KAF2074928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU91"/>
    <dbReference type="OrthoDB" id="20243at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="435"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="183"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="218"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="736" mass="82180" checksum="2A18940B6E766B0B" modified="2022-05-25" version="1">MKKLNSHTVNSSNDKHNQQAAVIDDINTNSQSLSKKKNLMSSFFKTKSSSSPNMNSYINQMNNNKLNSNSNNSSNSHTTTTNSNQNNSNQINTSTNNINNNNSNSSNSSNINHSVTVKVDNAPTILKKSTNSNPVISASCPSRSNSTLSDSLSDSLSGSPQSESEELLTRSGTRPFKSNTVGSTLNSNSSNQNTLTIMPNNNNKNNIVATAESNEKLNSSTNSSTSSASSSSNKTFTIISKPSSETSWEAPSDYDIEGTKINSIKDIEFILSRLSATQKTLNDKSSTNGANVSNKNISNLTASLNPQISAEEDLEKTEKKSKVRHDISVITEIFKHYNFGYDEYDLLDGDVQFHSMLEDEMERKTFLLAKQVLFLKMEMNKAVNPKRAFILNTVITSISKFLSTGTVPKQIPAIPLEMTITMGLNNSLLPHINNTIYKKGFLTKRKNRTLGGPKFSQKWVVLSHSEIFIYSSEKEDQLLIKKPLNELSSIQINSSQKEGLPSNSFTLFFSNIDFNNEIIEEWGTASLSNSFSSSSLSASSNNNGSSVSSNSNKKKTMTFTCESQDDLAQWVLAIDGVSNSNFKSTMDLNQKELYLHGYKTNYKGGSFRFGEEEWIYCDGKLTNQCWGDLLEYHWDGCTLVPSPHSKLSLGKGRWNGVWLTWYCEEKIKEPYLKFLYQPNQKQYIVDSFPIKNQYIWTWSRHFLVSSGHGGEWIVEGDVPPVLVMLVQMMKYYRVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUG8</accession>
    <name>A0A8J4PUG8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase II subunit beta</fullName>
            <shortName evidence="2">CK II beta</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_004848</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the casein kinase 2 subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000179">
        <property type="protein sequence ID" value="KAF2073852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUG8"/>
    <dbReference type="OrthoDB" id="3971593at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1820.10:FF:000005">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.20:FF:000002">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1820.10">
        <property type="entry name" value="protein kinase ck2 holoenzyme, chain C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016149">
        <property type="entry name" value="Casein_kin_II_reg-sub_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035991">
        <property type="entry name" value="Casein_kinase_II_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000704">
        <property type="entry name" value="Casein_kinase_II_reg-sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01214">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00472">
        <property type="entry name" value="CASNKINASEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM01085">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57798">
        <property type="entry name" value="Casein kinase II beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2073852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="238" mass="27362" checksum="6D2C0E9D9797E889" modified="2022-05-25" version="1">MDRIPKMNKESSSEEEDSLNEDEMAWIPWYCNLKGNEFFASIDEDYIQDDFNLTGLSSLVPYYDSALGIILDSDPEDPLSEDQQETLERSADILYGLIHARYILTPKGLAHMHEKFKKGEFGRCPRFYCQNQPVLPVGLADIQGVDTVKVYCPRCNDVFNPKYRRHSHIDGAYFGTTFSHLLLITYPELIPTKPPQQYIPKIYGFKIHKSARDRHIKKTPTNTANNNNNNNNNAPINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUN7</accession>
    <name>A0A8J4PUN7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ADP-ribosylation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_003474</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000109">
        <property type="protein sequence ID" value="KAF2075213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUN7"/>
    <dbReference type="OrthoDB" id="24754at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003500">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="18"/>
            <end position="25"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="120"/>
            <end position="123"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2075213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="19646" checksum="DEE6513F20F4B7B3" modified="2022-05-25" version="1">MNFSEIFPKKDMKFLMVGLDDAGKTTILKKLELGEIVTTTPTIGFNVESVQSKNTNITVWDIGGPDKIRPSWRQYYQGVKGLIFVVDSSDLDRIQEASEELHKMLNEDELRDIVLLVLCNKVDLQNSMDVTEVESKLHLYSIPYHRWFIQGTCATTMDGLYEGFDWLSKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVH0</accession>
    <name>A0A8J4PVH0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MACPF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_004395</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000154">
        <property type="protein sequence ID" value="KAF2074301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVH0"/>
    <dbReference type="OrthoDB" id="21110at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022829">
        <property type="term" value="F:wide pore channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052784">
        <property type="entry name" value="Perforin-1_pore-forming"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46096">
        <property type="entry name" value="PERFORIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46096:SF3">
        <property type="entry name" value="PERFORIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035239339" description="MACPF domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="domain" description="PA14" evidence="4">
        <location>
            <begin position="309"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="MACPF" evidence="3">
        <location>
            <begin position="511"/>
            <end position="858"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2074301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1132" mass="126314" checksum="BD113C7349D3B8D4" modified="2022-05-25" version="1">MRYLILTFLISSLINLILGLDASTNPTFYLSSNSLCQSECGSEEKPFSTFREAINTLSALKLDSIVLLVDQGEYYGQDNQQINIDFNLEIRSKNPNYKTIIDCQSTSYAFKAIGSTTLLIKGFIIKNCVAFKGGAIYTENQETTLENIIFMSNSAQLGSAVFSSAEVLDIKSCLFLSNLGSSSIIEINQSFSKISNSRFVNNLVKDIQCGPMATRVDSTESYFTSSCSSACEMYDQINSGSLCNNTITSGLSDCNLDGICDLFIETNENCPSDCTADQVLDQQFCNINGICEPTLGESFKNCPIDCVAELVPGWKFEKFDYEISKPKQTFTNYSNPIEIDFLSFPSIIALIGKGKVLISGKLSSKLSITETRDYYFKLQTSNLATIVYLDGRVLFDNFFKTNGDENQMTFERKLLLSSEKPHFIEVYFTATNDFQRELDLFWRTSSQDEFSLIPSSFISLIEKIECGDGICNEEPSTCLIDCHDQFEPNCSPTSPALPLQSYYYPIQDTIGTLLNTQYLSTLPGIKYMSQGIDLSTGKSKPTALFAHTYCDETSFSVVHFHYRDTVYTVPPGVNAQISPQCTSDSTMKFFSSASAFSKNEAEKWGISNNINGNYKAVTVAVSYGHSENSKKANETQDNFDRNFYQSTVLCKMYKINMVEPYRWNPRFIQDIGNAYYEHEDAQYAKNMTTENMIKVINNYGSGFYKSATLGGKLEQLTSIENSYLQKVSSYEIEKNNNYALAVSVSAGIFKGKASQRGSIDKSTSQAQMEEYIKNSKRSTLTVVGGAPGSYGEEEPNAFETWTNSLDKLPAPIEGAIGFVSDILPSSWYFKGGFNVKEIWEEAEQRIFKGLLKQLKFKYTNPKDASIDSLINSLGKTQGLYVLIGCSFNSASKPVKVKIENQATPFGFTKDVLFATPADKSFSLFGLDLNDEQGLRSIQFIGSNGNPIVLSSCDEFKLISTLSTRQYRMRPAGSKFVNSKPPPNELTIQFTDFKVDAPCADFYCGGLKRTIFGSHGKYTDALISGTFAPTILPKYLGDIIGISFHFFGDAFDKFSIPKYYQYTVSFKSVLISQTCPDDMKFGCIPNKVILDNQIGYTKTYETWRIPSRSELDHFQFVVNHTSSAYISIDPFGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVS4</accession>
    <name>A0A8J4PVS4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA-5-taurinomethyluridine 2-sulfurtransferase</fullName>
            <ecNumber evidence="4">2.8.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_002766</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>5-taurinomethyluridine(34) in tRNA + S-sulfanyl-L-cysteinyl-[protein] + AH2 + ATP = 5-taurinomethyl-2-thiouridine(34) in tRNA + L-cysteinyl-[protein] + A + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47040"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11726"/>
            <dbReference type="Rhea" id="RHEA-COMP:11732"/>
            <dbReference type="Rhea" id="RHEA-COMP:11733"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:87171"/>
            <dbReference type="ChEBI" id="CHEBI:87172"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.14"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MnmA/TRMU family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.14" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000080">
        <property type="protein sequence ID" value="KAF2075918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVS4"/>
    <dbReference type="OrthoDB" id="3685at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061708">
        <property type="term" value="F:tRNA-5-taurinomethyluridine 2-sulfurtransferase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01998">
        <property type="entry name" value="MnmA_TRMU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000104">
        <property type="entry name" value="Mitochondrial tRNA-specific 2-thiouridylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.280">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004506">
        <property type="entry name" value="MnmA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046885">
        <property type="entry name" value="MnmA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046884">
        <property type="entry name" value="MnmA-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023382">
        <property type="entry name" value="MnmA-like_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001138">
        <property type="entry name" value="PRK00143.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00420">
        <property type="entry name" value="trmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11933:SF5">
        <property type="entry name" value="MITOCHONDRIAL TRNA-SPECIFIC 2-THIOURIDYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11933">
        <property type="entry name" value="TRNA 5-METHYLAMINOMETHYL-2-THIOURIDYLATE -METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03054">
        <property type="entry name" value="tRNA_Me_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20258">
        <property type="entry name" value="tRNA_Me_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20259">
        <property type="entry name" value="tRNA_Me_trans_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="5" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Rhodanese" evidence="13">
        <location>
            <begin position="56"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2075918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="451" mass="51616" checksum="50BDD778FD238CDF" modified="2022-05-25" version="1">MLLNNIKKCINNSKIRYRYFCTTSTSSQKNNGNDYKSTANQRDRESLVKLFHDNKINFPSMNIVPKKPKVTIGLSGGVDSTVAAYLLKKQGFDVNGVFIKSWDEIEETGRCTGEKDFKDAQEAAKFLNINVELADFTKEYWNRVFQHFLKDYENGLIPNPDTLCNREIKFDTFIDFAKERFDSDYIVTGHYANLDYLNNNSSNSNNSNVHLYRGKDYSKDQSYFLCMSKTEKLAKSLFPIGQFMKNDVKDFAQYLGLDSIVSKKSSRGICFIGKKSLPEFLSQYIKVEPGRFYDLSNTYEIRQEHKGALCYTRGQKAQIHSLSERYFVAGTDIERNLVFVCPESVSDKFLLSTSVTMHSLNWIGVDPDQLFSNGKSIRIRSQIKHRGDLVDSTLCRQNNNEYTVTFDTPIRACQSGQVLCMFEINSDRCLGGGVMKLPPFKVGKQTFGLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVS5</accession>
    <name>A0A8J4PVS5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000139">
        <property type="protein sequence ID" value="KAF2074590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVS5"/>
    <dbReference type="OrthoDB" id="10685407at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="315"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="464"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="169"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="194"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="220"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="315"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="464"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="639" mass="73281" checksum="A81481AAD9C53E4D" modified="2022-05-25" version="1">MSSASPATEQELQYNISKASQKVNSFLKNYPERQWSEAVKLILLAGVHCIQEHFGLPTPSIDQLRSLFISDEKSILDMIIKPTPIKNKQQHQHQQQQSMNNNSNSNNSKTMVPITVATPTATTTTTNEQEKDIYKTPTSMKQTIKLKPSIDDNEDYLYRNSTNTNKMGTSQLQTNSTTTTTNQIKQQKQKHPINNNNNSQFKQSQMNNASQFKQSQPTSNTKSQYLQQSQQQSQINSNNNTQIKSILVNSNHKQYKFESPSSSKIRFQENPYVEEYNNGDDEFDNNNDTFNLSNSMFNDKYNAMFHSLDNRNNNFNNSNINNRSSNSNSKFYHHQEEEEQHEQEEEEQEEYQYEEEPTQLASYYQQKQQYDDDDDGGDYQENNGDSHFEIPESQLVNSNGESRILSKSTIDSINSNKTFDSINQELDSLSLSTTMINSGNTPSSKFNQSMTKHQEQYSRFFQSTDYNKQDQQQQQQQQKKSTNVNNSNSSTNNKSPTLPMDINILSREQFQDQENTLNKTVVPATTTTTSSINNFDVSPLSSSTISPYSSSSSNNINNNMSITSTSSPFNKSLNLSLTPRSNKYLSSLSTSIPSLQQSIPVVNHTHHHYHQQQKLQQQQQAQQFFNYMEDELEVIELIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVZ7</accession>
    <name>A0A8J4PVZ7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rab GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005645</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000231">
        <property type="protein sequence ID" value="KAF2073029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVZ7"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004294">
        <property type="entry name" value="Rab-type small G protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="227" mass="26431" checksum="D098247CD9E1939B" modified="2022-05-25" version="1">MDSTSSFESKVVLLGSSDVGKTAISLRYAEGIFNKRPTPTIGASFLTKTINLEGNKIKFLIWDTAGQDRFRSLTPMYYRGACVAILVFDMCLQKTFDTVKEWVEELKANIQEEIVMVVCGNKMDLQHKRQVKRETAKQFADEIKAMYFETSAKDNENIEPMFLEIAKKLIIDKHTKYMQEIQKQQQLHKQYQTQNQSLYRQQIQQQQLQQQRNINSTYENDNNQCCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWA0</accession>
    <name>A0A8J4PWA0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4326 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003779</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000123">
        <property type="protein sequence ID" value="KAF2074902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWA0"/>
    <dbReference type="OrthoDB" id="272703at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025475">
        <property type="entry name" value="DUF4326"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14216">
        <property type="entry name" value="DUF4326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4326" evidence="2">
        <location>
            <begin position="64"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="233" mass="25776" checksum="DBB0493B2F50A7C9" modified="2022-05-25" version="1">MYSKKQTPSYNYSNNKSTSTTSTTTTIKSHHHDKEPIENTKPITPSGVYLYNEDYTHFCVNCKTSKDYDVYVGRKNPTIKSTVSTKWGNPFKVGPDGDRNAVMKKYRDWIFSPAQDDLFQCAKTELKGKILACWCKPYNCHSFILAEIANSSISNKSPVVDVQVASSSPSTSTTTTTTSTASTSPSVKPPTPTMAQLLQSQPKTNNIIVAKPSPVIESDNDFPILGKMITKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWC9</accession>
    <name>A0A8J4PWC9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2 5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_003451</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2 5"/>
    <dbReference type="EMBL" id="AJWJ01000108">
        <property type="protein sequence ID" value="KAF2075233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWC9"/>
    <dbReference type="OrthoDB" id="1902587at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044609">
        <property type="entry name" value="FKBP2/11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45779:SF15">
        <property type="entry name" value="FK506-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45779">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0697">Rotamase</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035243668" description="peptidylprolyl isomerase" evidence="8">
        <location>
            <begin position="21"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="148"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="9">
        <location>
            <begin position="43"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="172"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2075233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="194" mass="21088" checksum="9E32BAB8418D1A41" modified="2022-05-25" version="1">MKISIISLVIFALLASVVISQKVELLSSSMQNEKCPTPLVKLHDNVSVSYVGKYEDGTVFDSSEFHGPFSFVVGGRQVIPGFEIGVMGACLNEKRTIKIPYQLAYGEQGIEGKIPPKADLFFDLEIVDVVAGPDVPLYVSLIPSGSTIGAFVILGIFVGAVYYIVKLYPGNADEKPKPHVKQPKEKKNKKAKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWF1</accession>
    <name>A0A8J4PWF1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003805</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000124">
        <property type="protein sequence ID" value="KAF2074886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWF1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="99" mass="11428" checksum="308EC2DB59925F76" modified="2022-05-25" version="1">MRPHFQPTEIKSLSFDKFTTCVQINHDICSHDYRLRFTANTINCLRECVLVCLETIDTSRLNGFTFPDQSVDEMKAASLPILQEYMALKNIEPKLANDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWH5</accession>
    <name>A0A8J4PWH5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003385</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000105">
        <property type="protein sequence ID" value="KAF2075308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWH5"/>
    <dbReference type="OrthoDB" id="10002367at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005683">
        <property type="term" value="C:U7 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071209">
        <property type="term" value="F:U7 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006398">
        <property type="term" value="P:mRNA 3'-end processing by stem-loop binding and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039267">
        <property type="entry name" value="Lsm11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21415">
        <property type="entry name" value="U7 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21415:SF1">
        <property type="entry name" value="U7 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sm" evidence="2">
        <location>
            <begin position="150"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="106"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="244" mass="27322" checksum="38065979CF6A2132" modified="2022-05-25" version="1">MNVETKDDNTIQDQRFNVLSELFDPLLALGSPSKVKLPNPNIRPLDNIHKARILLPPNDENYLQIKPKLKPLPNNSISSSSSSVNSSNTNSNTIKPVSPPLPPPKTTTTTTTTATTTNTPTPAPPQENKNQQYKVLKNISSKFTDGPLNVLKKALEMKKRIKIIIRGVKSIRGYCVGYIVAFDKHWNIILRDVEEEYTIVQYIDNNIPAASIDEKPTTKVYKQIKKHYGQLFIKGDTIVSVYAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWJ6</accession>
    <name>A0A8J4PWJ6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Citrate transporter-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_003602</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CitM (TC 2.A.11) transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000115">
        <property type="protein sequence ID" value="KAF2075082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWJ6"/>
    <dbReference type="OrthoDB" id="442352at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015105">
        <property type="term" value="F:arsenite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000802">
        <property type="entry name" value="Arsenical_pump_ArsB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004680">
        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43302">
        <property type="entry name" value="TRANSPORTER ARSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43302:SF5">
        <property type="entry name" value="TRANSPORTER ARSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03600">
        <property type="entry name" value="CitMHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00758">
        <property type="entry name" value="ARSENICPUMP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="177"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="359"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="395"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="444"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="479"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="525"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="Citrate transporter-like" evidence="9">
        <location>
            <begin position="31"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2075082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="545" mass="60512" checksum="2F8D1AC1D932A297" modified="2022-05-25" version="1">MSHVIELNDGAKKYFSGFIFLISLILICTKIERFPIGRAAVSLIGSTLMVIFNIVPPEEIKNAINFDTLILLMSMMMLSNYMEKANIWDIASKILLYKCKSPVVFMIRVCLISAVLSSILTNDTVCVTLTPIIITACKQSNLPLFPYLMAVATSANIGSAALPVGNPQNMIIATASGLSFITFFKVSVVSSLIGIVINSSLLYLYFRKSLLSGSKGKDGYINTNDHSIIDDGAPIEASIIYKEQDNSHHIEKINDNYIVNIRKVENVQLEQDEEEEEDQIELESIQLDKSIQHNYPNRNNNGAILVSNSEMDDNNDLELLNKNTSSNKIYGSDIKSNLNRYYEKYKPFLSMLYFYKPGIIIILVLIGFFLGFHMGFTVMVGVSILIVLERKDINDVIVSVDWSLLIFFSGLFVLVDGFDREFSSLAWTVLEPFVPLASSSVLKILVFTIIVLVACNILGNVPLVLALCPRLLEEMAPDFTWLLLAFVSTVAGNLTLIGSVANLIVAEKAKFDYQMGFYEYLKFGVPSTLLVIVVGVPIVVLIAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWR5</accession>
    <name>A0A8J4PWR5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RNase III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000138">
        <property type="protein sequence ID" value="KAF2074615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWR5"/>
    <dbReference type="OrthoDB" id="20199at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="353" mass="41221" checksum="01CF803AC811C574" modified="2022-05-25" version="1">MMKRFITNVLVPQHTTCLFNLSLSSYTTTRLATSAVAIKSKKEHTYSNKQKKYNNKKDNHNINSNNDDKNQRPPQPQQQSPLHQTPRVKEEKKEDFIYKYGSKIESEQEIGKELLGFIQDRFKIDTSSTTNTEDFILAFTNFNSSITASGQLNLAANQKLMYFGKLCLGKTIQDSSSFQFEEDIHRLEFRRSVESSFFLRKRFVSLGLDQFVRFNLKKQQIKNIEYYQNHYYYQSMVKFLGALYLEFGYIKTSLFIHQFLLEQPTKPLESILLDMPNYTLIKRFAMYKLEHPIIETSAHLPLYETTIKSGRTVLQTGFGLTRDDSKYSCMELLNQQTNTLGETLSLILRKTKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWS5</accession>
    <name>A0A8J4PWS5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribonuclease P/MRP protein subunit POP5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_005347</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the eukaryotic/archaeal RNase P protein component 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000211">
        <property type="protein sequence ID" value="KAF2073329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWS5"/>
    <dbReference type="OrthoDB" id="24745at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030681">
        <property type="term" value="C:multimeric ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3250">
        <property type="entry name" value="Ribonuclease P, Pop5 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002759">
        <property type="entry name" value="Pop5/Rpp14/Rnp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016819">
        <property type="entry name" value="RNase_P/MRP_POP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038085">
        <property type="entry name" value="Rnp2-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15441">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15441:SF2">
        <property type="entry name" value="RIBONUCLEASE P_MRP PROTEIN SUBUNIT POP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01900">
        <property type="entry name" value="RNase_P_Rpp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF023803">
        <property type="entry name" value="Ribonuclease_P_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160350">
        <property type="entry name" value="Rnp2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR023803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2073329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="161" mass="18800" checksum="DBC722E006CC49D3" modified="2022-05-25" version="1">MVRLKNRYLMTEVIWHDPSNASALSELWLFHFLIDESKRLFGELTTEAFKKTVKFVYLNPDTNLFIIRCSFTYYKSLWSVLSMITSYNNIPVYFRVIHLSGSIRGCQKAALKVFQKQIFIYDKNTIQQQQQTSTSSLLSITQSDIEMDNAPSEDDDIEMKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWT6</accession>
    <name>A0A8J4PWT6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">S5 DRBM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_005335</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000210">
        <property type="protein sequence ID" value="KAF2073344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWT6"/>
    <dbReference type="OrthoDB" id="309483at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_uS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_uS5_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="RIBOSOMAL S SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718:SF61">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US5M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="S5 DRBM" evidence="3">
        <location>
            <begin position="760"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="133"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="577"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="679"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="137"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="681"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="714"/>
            <end position="733"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2073344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1009" mass="117189" checksum="CBF5B7D6C500AC6A" modified="2022-05-25" version="1">MNSQLTKITKDLRTFLTVRNNVLLLNNSSRYYSNTTTDKSKIQVQREKATQDKQETNQANFILDNLDIQYDKNSYNKHYTNDLGKKFMSPYSGRQLNYYPTTLEFLTNGYAATESLYAVENIKELGVKPAPRKRIVDEDEEDDEEEDEEHSNTPPEVDITRVRADYVDEMPRVEKQSLLLIKRLGKILHNPLKHKAFYLMSLDQQKFFEKACQLLENYDQDTYDESNEDQLLQEYAALVEKMTERQVIREQRQRKADENIWKQFSANASVLVQEMRKAKIDVREAIRSIDLMDEFKQLHQQKQDKLEHALAKSSTIPQEQEDEKLTYVAAVRPGDDLLFTALYDASPVIRQQFDDFVELVEKMIKQNELAPFNLEYDEVSIPDEFESWAQGEDLDTKELVPLLIFVCERIIAGFQNTAIYRAGPQAPAVEGQLTYDQVCDWIKLFRYFRARGMGFIKYEEKGVKEPVKTLAVSVEQEMAEFFDFINLYKRYSTKPHMFKTFFNVMLYAKWNPKNFGASFYQSTLRKSEKEYLSYEWPDTASFGFHDITIHDLFDPLYRPHIKRNMSLLDIENDEYDGYEDEQQKTGAAAPPGSKPDPEENDEDLQDMLEFLKEVDKKQWDENDPETFFPDIEPEIEPSNYKSSLFKDRGYSDAIAEAQSDFLESASNYFAGERGKELTGFDLDELNDNEEGADEDEETGVAYNEDESAQLGNEGEQEQDPMDEFNQDDGEKDEFEDDQLGERFEGGRSQTNVYRDVPVLDISNIVNVARHVKVTKAGRVNSFSCTVFNGNGNGTASLGYGKGDSPAVALKRASRDAERNAFTLDRYKGVTLPSDLELHYRSTTIKFFKSRPIDQIDTRGHKQGIILPTLGLHGVNFRTYGRRKWANVLLTMQRRLEDFTNPEEAARLMGKKLVTHNSQKEKKKTLSELLQSKIHKKNTDPYNIGLVKDFLYDMERPNKEKIRDRKIQQDIDEVLDNYPKDQEDSDLYTHKSTKFGQHLYTDTKQVDRMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWZ5</accession>
    <name>A0A8J4PWZ5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_004283</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probable adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000148">
        <property type="protein sequence ID" value="KAF2074427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWZ5"/>
    <dbReference type="OrthoDB" id="6105938at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001293">
        <property type="entry name" value="Znf_TRAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131:SF65">
        <property type="entry name" value="RING FINGER PROTEIN DG17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131">
        <property type="entry name" value="TNF RECEPTOR ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02176">
        <property type="entry name" value="zf-TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50145">
        <property type="entry name" value="ZF_TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="94"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="TRAF-type" evidence="11">
        <location>
            <begin position="244"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TRAF-type" evidence="8">
        <location>
            <begin position="244"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="9"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2074427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="40558" checksum="19E927051FBD5721" modified="2022-05-25" version="1">MGNTLNTQNNSRNNNNNNNNNNNNNNNNNNNNRSNDDDINNNNNNRNRNSLERDNSSSSSHSSSQNDDGDFNVNNLDQLNLEELLISNRENYTCKICYTVASKQYQCGSGHYYCLDCWDSHLSRSNDRACPECRTVVSSLVRNLFYETLLANEIVHCPYYKALVHKDNRTPCLGIKYSDLESHIKYQCQYKEQVKKEIKAKSVSGKLWGFLGKGFNSLLLLTKGQSMVKCRHCHMLRERKDIESHVGICTEFIVDCELCGIKIKRQDINDHRKFSCEEAEIKCPCYKGGCCDSNIKRKNLQDHLKLYNNHIDYIVHMNYYLSNQQDEIKTLNDKLNEKDTLIAHLYSSSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXP5</accession>
    <name>A0A8J4PXP5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007297</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000381">
        <property type="protein sequence ID" value="KAF2071381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXP5"/>
    <dbReference type="OrthoDB" id="22671at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF180">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="273" mass="30537" checksum="AED2D54AA96D20E8" modified="2022-05-25" version="1">MGIGKSKQTGQIDRLVNSIWRNSYFRGLNNLYSKSISINAARAAGAQGPTFLILDQVDQQLDKDTLPSTLTNLSINKYNQPLLPNVLPKGLKTLTLMGFNQVFSAGVLPSSMTDLSLPNINRQLKPYSLPDSLTHLDIKQYTGSLSFQVLNELSTLEIKHLYPCVTAAIWGTNSITITCNKFSPWINLQQTPIQHLKIHLNSPDRVTLKTEFIPKDIRSLRLCGFKIESPGLIPKSCLYLETDIIDLNTEFIPSTTIHTFYDDKGKDDVSNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXX4</accession>
    <name>A0A8J4PXX4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_002827</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000082">
        <property type="protein sequence ID" value="KAF2075891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXX4"/>
    <dbReference type="OrthoDB" id="21449at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04369">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45926">
        <property type="entry name" value="OSJNBA0053K19.4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Bromo" evidence="8">
        <location>
            <begin position="519"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="781"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="427"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="794"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="809"/>
            <end position="826"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2075891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="826" mass="94457" checksum="8794EB31F0E7A2EA" modified="2022-05-25" version="1">MSNFHNPLAPLPLPIPNGLSNSNVTSPVLSPKLTPAKDTTLMNQQKDRYGAHLFHKNEEGFYTCFFCRKQLQANFSRHISKHEQDGDPIDPSFKIVGTSASSNTDTVAPPSPAVIAKPQPPPPTIIPQPQTQPQAGMMGMPIAPQNINPFQYNMINASTTINGTAGVYPNLTPLKNPMAMMNGMMTTPPPNQQPFIHHQTPLQPIPFHIPIPMSMPMISPGNPLFQGGVPSQHIQPSPIKSNPTRAAAAAAAVAAQQQATDAQADIGPPETKPGVIYQVCQHPNHQKWWGISSVLPASEFYPRASKCKKCYIKQQQDAKRSKQTPNSMMSIMNSPHMNSMSDHPEQVSANKLIQHQIQQQLVQHQLQQHQLQQQQMQQQQQPQIHAGMVVKPMVTPGSLPLVIQQSPIVPPPSGFHLQYQQTQLRQIQQQQLQQQQIQQQLQMHQQQMKQSAYTQQAQQQTHQQAQVQQQAQQAQQQAQQLQQQQLHSKFLFRPKIKIDTGKLNDIILDKCKHLLLTFTKDPRSKPFREPVDTVKLQIPNYLKIIKSPMDFSTIEADLDESKLTYQEFYDNMLLVFNNAMLFNPKDTDIHELADELEKEFIDQYEKILEDAKNQLLLEEEFVQDICAYCKMPKREPELIDPATNEYTEPTPLIPCHGSCLRVFHDRCLNVQYDPKLLHNFVCKECFQGKPVYHYDPQSSEYFYYYYDPLDEEIYLEQQQLVLNHHHNVQSMEEDEEIVSDIGMLNKEEKINLRKLEETYREEEMVYDPIWCMWRSKSNVEQKDRETRQKPIIGTVTSPLINSSQNSLKKGAKPTVGKKKRSAKKRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYH5</accession>
    <name>A0A8J4PYH5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_002411</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000066">
        <property type="protein sequence ID" value="KAF2076296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYH5"/>
    <dbReference type="OrthoDB" id="1560166at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000039">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF7">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE-LIKE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="8">
        <location>
            <begin position="11"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2076296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="338" mass="36738" checksum="4AFDA0EB62C0403E" modified="2022-05-25" version="1">MMKCALFPRPGAGIELASIPIPEPKDQEVRIKVYACGVCHTDITVGEGKVDGITYPRVPGHEVAGVIDVVGKNVVGFKKGDRVGVGFYGGKHCGICKDCLNNSWTTCKKIETCGTTYDGGFAEYMVAPQDALAKIPEDLDFKYAGPLMCAGVTVYNSMRNMKMMPGSLIAIHGLGGLGHLAVQYGNKMGMEVAAIGLDRTNEELYKKLGAHHYFTNDEAVEKLTALGGANLIVATAPHPPSIEKLQKALANEGKLLVLTLIDSMTIDTMDLVLKKKSIVGFLTGDPRDMEDTLKFSALTNVKPMIEEYPLEKVKLCMDKDHMAKAKFRNVLIIDKEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYU8</accession>
    <name>A0A8J4PYU8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 4E</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002184</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000058">
        <property type="protein sequence ID" value="KAF2076506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYU8"/>
    <dbReference type="OrthoDB" id="590761at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF65">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="197"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="197"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="213"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="227"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="296" mass="32833" checksum="44C9E25E7CFBD9E3" modified="2022-05-25" version="1">MNLSYTDQMEIQAKSQKPTQHRLDCTWTFYTDKKQFNTGITDGDYMSTLTNIGSFSSVEDFWSYYHKLQRPSRMQADTTYHLFKSGVNPIWEDPENINGGKWVINFTKQNRFIFNIDLIWEDIVLGIVGETIDTDRDICGVVFSKREQIERIAIWNKDCSNEKNIEALKTNLLNAISGGGMVDVSKLNIRYQSHSNTLNSSTTSAGSNTGGNITPSTSSTNLSAVLNSSNSTTPIYNSSSNTNPNTNNSSLNNNQNNPNANNSLNQNNVNNSKSFPINSSSSISTENISNAATATN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYV7</accession>
    <name>A0A8J4PYV7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003639</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000116">
        <property type="protein sequence ID" value="KAF2075072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYV7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="353"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="373"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="386" mass="44216" checksum="42196FB6D95ECBC9" modified="2022-05-25" version="1">MSGKQKVNLYDLQSQSKSNLPTGPRSAAEKEEQANSGYGRDTRDYRPRREYSSPQYSNSFSNVEKKPVQSSEDDFPPLGGEVKKPAPRGPRPVQEKSDNPSSPSASEDREERSSPASEDRDERRDDRDSRDRDEPYRRREGGYGRDNNYGGERRDNYERRDNYGGDRDNRRDDNREYDSRFSNGVSPFGGYKRRDNYGGDRDNNRREGGYERRDNYGGERREGGYERRDNYGGERREGGYNNRRDQPQAPFERREYVSKVDSVDDWRSPSSDVSTNAFNKLSINDKKREGGYGGGEREGGGSYNNKAFGNNRYNRDDNRGGYGGGDRYNRDDRGSYGGGDRYNNNNRGGYGGNRDDNRGSFGRSSEDRPQRDSPPQQQQQQAPSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYZ8</accession>
    <name>A0A8J4PYZ8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000095">
        <property type="protein sequence ID" value="KAF2075544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYZ8"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="335"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="872" mass="100213" checksum="3F11AE86FD7DAA2F" modified="2022-05-25" version="1" fragment="single">MVIYDPWVTPSYNQGWGTGEDDDDTKYFIMNIFSDKSGRVDKKKLNEIPEGSTSIRIVINRQKDDIDRYLDLVKGYVPSSVTRVNIEYNYPEKIEPCNGGDGWLPDSVTMLTVPSLPFPAALVPKNIRYLKVQKQKKAIEIDMVPPTTEYLWIGGPSKTNGKIMVHSIPNTVKELVVDSYVLDEIRIKPTFTSPIHISNPRMYHDGDRFAHVGLKNTLCNLNIQTITSSTPVIARLPTITSFVIEEHRSFNNNLSALHPLPPQLKYISLPSTYNQPIYQQQNLPISLTHLELSVIIDENNHFTNNWLCRKGYLPPSITHLKINIYFEGYYKVNKNNEHDNDEQDDTNNDNDDDDDVELETKEEKEMDIERNVDKELNDQDDEIEIDSNSTHPLKKRKIDGDQDQLVTKQDEYNEEEEGYDPLPSSLKILIPSSIQSVCFNIDHKSYRSHVTVLDKDDYYSTTPTPPKMRQGLKELVLPESFNNHIHNSTLPSTLQSLSINNPDYNNPLNSNNLPLSLTSFKCNSDKIKLDLSLFKSIKELKVNVDAITSYPPNVETLELISPSEKGYQFQFNKHLIKKLLDTLPNVQYLSSEYLDFKVPASVQELKCLDNSFRCTDARPISSPPFETLHLDLDYYLENHSQTQDNHQRGEKIINYHISNQQPMLNTFFKIWRNIYLKPLVLEALYDQTKSPLRFDSKTGTLEHIDKFKQYNIFVKDVTQLGPALLKFDKADEINFLGEENDTINTNLSRFISKSIKKLRCSFTKGIPDWITHLNLGNIRFCDLQVSDIPPSVTNLTILTNDIGYIGKIPPSVRYLTLNSLMDNQAHCIPPSVTHLYLVNTAFNIINKTDIPSTVQRIEFKKLFYQKDKGEYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ14</accession>
    <name>A0A8J4PZ14_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome P450 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_003117</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000094">
        <property type="protein sequence ID" value="KAF2075574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ14"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20617">
        <property type="entry name" value="CYP1_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000068">
        <property type="entry name" value="Probable cytochrome P450 508A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF417">
        <property type="entry name" value="CYTOCHROME P450 508B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0349">Heme</keyword>
    <keyword evidence="9 12" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="430"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2075574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="494" mass="56861" checksum="6290933F8EF3EBE5" modified="2022-05-25" version="1">MSIFWLLISVLITLIYFNYVKKLKNYGPKSLKGPMPLPVIGNFHNLGCTAPHIPLFKFHEVYGPIYRIWMGDTYTVVLNDLDLIRKMLVHNASNFMERLFTPTLEFYSGDFSSLAFSKMHDWKDKRNIVSRSLVKNKAKNIEMVSNQVDNLLDCMEHYCKSNKPFPPRKNMQRFAMNTILKFVMNQEIPYDHQGVKDGPMKSLLTECDKVFIDLGTGKLGDYIDFMKPLLTLHYKWFDTKANKLRGIVLEEFKNHLSTFELENKENPRDLMDVMIGEFEGGESMDSTAVSMTLDIVLAGTDTIAGSLEWCLLFMANYPEYQEMAHKELEIVVGKGRKSMLADRISTPFVNAFIKESSRYKVVAAFSLPRVCSEDIEIDDYFIPKGSQILLNHTGIARNPEYWENPEIFDPTRHLKKNQEFSLYGYGPRNCIGQSLAQDKLYLAITNILLRFKVGSVDGNPIDETEIFGLTLHPNLFNLKLSEREKNIINSKKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZK6</accession>
    <name>A0A8J4PZK6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_001764</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000044">
        <property type="protein sequence ID" value="KAF2076926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZK6"/>
    <dbReference type="OrthoDB" id="9049620at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904294">
        <property type="term" value="P:positive regulation of ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16532">
        <property type="entry name" value="RING-HC_RNFT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044235">
        <property type="entry name" value="RNFT1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15860:SF0">
        <property type="entry name" value="LP20373P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15860">
        <property type="entry name" value="UNCHARACTERIZED RING FINGER-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="279"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="328"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="435"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="496"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="157"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="227"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="7"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="81"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
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            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
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            <begin position="157"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
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            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="241"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2076926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="557" mass="63733" checksum="44D9E7DACFF2BC73" modified="2022-05-25" version="1">MELSIRIDPLSNDNDDSNNNNSSNNNHSSLNNVHSNNINNINNTPTIPTTNNPFVPWNPTTTTTTTTTSNTSPSNIYPSSHFNTVLNFSLDNNSNNNSNSNGGVSLDEQPQQQQQQHQGRLSHLSHQFNQISSRTNSIVNQFIHNATNRVIQNHNNNIENTTDTFDSNNENNDDPHGFYNRSSNNNNSNNSNNSGNGLLNTNRFSNLISNDFRARLQLQQLHQQLQQLEQDADADDRQDQQQDQNQQQQQQQQQQDGNISNASTRVDIPMVLKWFEQNLMFLSLLSVIFIYVHKQGILVFLWQQIIFFQSNQTLQKQVSLQEKRNVGVLFWLILILSSNVLTTYLFFSTHELWRSLVFLAPLIEFDIWSTFWVVTVNDFVCRFVTMIVKSVCVIVVGHKPPFKRRAQLYAAIEAVSFLYRMLIPTSVWVCYIRGLALNGEYMFSVTVMVLYLSFKLNTLFHNTRVTILIVRAYLLSHTLYGKKASAEQVAESGDHCSICQEKMVSPIVLRCNHIFCEDCVSQWFEREKTCPLCRSPIQAAGHRTHSDGSTSLLLQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZS3</accession>
    <name>A0A8J4PZS3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002684</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000076">
        <property type="protein sequence ID" value="KAF2076024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZS3"/>
    <dbReference type="OrthoDB" id="21343at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="146" mass="17547" checksum="EE8F0E4EA77F76D1" modified="2022-05-25" version="1">MGEIEKKTLIQIETYYRDKLLNYQEKLKKDFENVHIKYIENIKYKDNDSDDNGETTIKLKELELWMKKSLLLILYEMKTFMTNYKQQQNNSNDNSHGQLESDKNIFNQLEMIASTVSNLLDQETQLSRKQYTEFETFLSEFCKSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZT2</accession>
    <name>A0A8J4PZT2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Calcium-activated BK potassium channel</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_000448</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000008">
        <property type="protein sequence ID" value="KAF2078258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="257992at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005267">
        <property type="term" value="F:potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003929">
        <property type="entry name" value="K_chnl_BK_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF22">
        <property type="entry name" value="ION TRANSPORT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03493">
        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22614">
        <property type="entry name" value="Slo-like_RCK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="84"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="13">
        <location>
            <begin position="95"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="15">
        <location>
            <begin position="314"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Calcium-activated potassium channel BK alpha subunit" evidence="14">
        <location>
            <begin position="448"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="15">
        <location>
            <begin position="850"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="664"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1047"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="674"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1050"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1075"/>
            <end position="1088"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF22614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2078258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1197" mass="136587" checksum="C58C03EA4C564769" modified="2022-05-25" version="1">MSSINNGDHIDELESSASSTSIGSLPPFHRPPGFNVIFQHILQLLLILFIVLFILTSITLLARLSRKTNRQRSKKLSLRTRTEVYLTATGIIQWIEGIQTFLSILSVFAFIYGTYLTHPDKPPMIYSWIEVALTIFFTLHWMLDYFIHKDRLQYLYSFFSIVDLVTVLPIYIDIAQGNFFNELTTFQFLRVLRTVRVLRANRVLHYFNDITQYLFRVVIAIFTFIMIVAAFYMNVDVKDKSTGEALKFHETIYFLVVTLTTDGYGDIHPTNAVGQITITVAVIVGAVLIPYQVSRLLETFSSYSPYKRDLSKENMHGHIILCGDINFTSLLEFLTEFYLEKYAQLKKTIIILSPAPPSDQLKILLLHPFYKNRISYLEGSPMLENDLKRARYIHSSSCFIFRPLFWEDGDSTAILTALAMKNLKSNGSKIFVQLLHHENKYKISSRVKHIMCIEDFRNGILVQSTLCPGFGTLVCNLFTSRQPNATNEEEKWVDEYCNGSVNSIYKIPVPLALVGETLGYVTSIMYSGAKALVLGIIDDTEDDTISLGSSSHIPNQHHANNHHHRRNRFVIHPPFSTILTSSMNLIIIAHSPSAPTFKPNVPSIIRRNEEDEEEPSSFIRNIYNKLFVPDEIRNFTELNESSADLFNDNVKTFFTSTYTTNNSIEMDNLSPTLNSNQDANNNNNSNEDNQDNGANPLPLILINNDSINNNNINNNNNNNPLEEEEKISPLVKLCNEEFDSNWNEVVATVIESIDGKAHMESIMEDLEPVLESIERNRNLDEYSEEEVEANNWKEHIIICGSINKIDLFIRGLRQKYISDNINHFYHLKNGSASSSTNLNESAAEHHSDHSSISVPPIVILHPEEPDMSEWDDIEDVFYHKGSPLERKDLDWCKIQLASKIIILSDPYKQHHSSNLTDKTSVQLDAETIMAYLEINEVFQNSPNSSKSSFLTVELVHEPNIRFISKSFKSSNYKKRMRREEEEFEKPNYYITSEFASGRVLTLTTLDSLMCQASHQEYLLDVATELVLGVNGIKKYYQKLIGSNVVGASDDSSDSSDYYDEDDTHTASSEDDLTNLDINSRNSSHHTIYNQSNSNNNSTPNLRLTQLGIFNHHNPPSSSAHNQCLLNQIKVPEQFHGHPYSELFEALLSKNILPLGLYRCREITGAPNPYVFTCPPSATTLHENDLVYVLYRQHHHHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0C1</accession>
    <name>A0A8J4Q0C1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001114</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000025">
        <property type="protein sequence ID" value="KAF2077573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0C1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035207564" evidence="1">
        <location>
            <begin position="21"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="75" mass="7664" checksum="1CC6570D9DEE8F67" modified="2022-05-25" version="1">MKLSLIFIISSLSPSKSVSSVSTAAGASGSFKMGLNMNTVNGTITSTTTTSTNANVAGKQVDANVHHQLNANITH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0M1</accession>
    <name>A0A8J4Q0M1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FMN hydroxy acid dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_002200</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000058">
        <property type="protein sequence ID" value="KAF2076522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0M1"/>
    <dbReference type="OrthoDB" id="25826at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02809">
        <property type="entry name" value="alpha_hydroxyacid_oxid_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000056">
        <property type="entry name" value="hydroxyacid oxidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012133">
        <property type="entry name" value="Alpha-hydoxy_acid_DH_FMN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008259">
        <property type="entry name" value="FMN_hydac_DH_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF107">
        <property type="entry name" value="2-HYDROXYACID OXIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000138">
        <property type="entry name" value="Al-hdrx_acd_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01240">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00557">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 7" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="8">
        <location>
            <begin position="23"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="102"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="307"/>
            <end position="311"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="330"/>
            <end position="331"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2076522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="384" mass="42311" checksum="5D239887F9F55A01" modified="2022-05-25" version="1">MSNNTITYDDIKSSDQFRNNKVDAMSQFVNINEFKIEAQKKLPKMAYDYYASGANDQITLADNENSFKRIKLIPRCLVDVSNVNIKTSIFGVDIKTPIMIAPAAMQKMASHLGELDTLAAAHEMGTIMTLSSLSTSSVEELSEQSKGNPGWFQLYVFKDRKISEDLVKRAERLGYTAIVLTVDTPYLGKRDADYKNKFHLPLNLQLKIFSHLPLSKLDGGLNNYMATMIDPALTWKDVQWLKSITKLPVLVKGIMCPEDAELSIKHGADGIIVSNHGARQLDTCPSTIDVLYPISKAVRGRVPVILDGGVRRGSDVLKALALGANTVMIGRPILWGLSVGGQAGVSRVIKLLNDELLLAMAFTGLNSVKEISPAIIYKHPLSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0S3</accession>
    <name>A0A8J4Q0S3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_000888</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic initiation factor 4G family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000018">
        <property type="protein sequence ID" value="KAF2077843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="20666at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003891">
        <property type="entry name" value="Initiation_fac_eIF4g_MI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23253">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4 GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23253:SF9">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4 GAMMA 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02847">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51366">
        <property type="entry name" value="MI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MI" evidence="5">
        <location>
            <begin position="1119"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="268"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="989"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1069"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="7"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="55"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="110"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="124"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="302"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="373"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="401"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="414"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="426"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="450"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="488"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="512"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="544"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="990"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1076"/>
            <end position="1115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2077843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1312" mass="146616" checksum="05B9B08566A75EA7" modified="2022-05-25" version="1">MNTGKRQPYTNNNNNYRQPPQQQQPHIYHQYPQQPQYHTYGPPPPQFGYPPYVGSQQQFVPPIHQQSPNIVHPISPQHHQYQPQQHYTNIPPPGYQQPQFHQQPPPTSPTGHSNNSIPTTPTGPSSVASPQSPVSSNVPPQQQPTTPSQSSTTTPTGTPNRKGQTPVKPSVNQHATMVQAIKRTTSAPPSLEEQNAKLAGFTPSGDKRILQGAPQPQQQVPPPYIQGYSPNIGKMPPNMVPSPQGEMMYPNQMFSQYGRPMYYPGPASPSGAMPLQTPSPPPKKVLDIIDPNTNEKVNPVNPSSSSSSSSTTTSTTTTTPSSPSTPAPVSATTATSPATTTTTTTPAATTSTPGYVYSFSAGNLNLRKKKDSTSTTPATESTTSTPTPATPTAAAAVVAATEDKKEEKKEVAKAETTTEAETTNKSVEKEAEKPKEEEKKEVAPVATTSTKEESKPKEKNETVEQQKSVESVVDAISKVSIKDEKVVESNNSSTSNDNSSSSSSKDVEKDSTTATTTSTSTATTATKQEEEEEEEWEKKAEEGLNLTPSTENLNTSSSSLPPSIPFRNSGEKIVYSKEIMMSLKPKNVEEPTILKDMKNQIETTNMPLNRSNNKINKGGMNMNMNMNMPQYPQQMNMKYPPQMNMKYPPKGFNPNYNNKYPQQQGGNFNYQQQLHQHQQQQYSMYNNQGVQPPISSQPVNENRWIPTKVAALSSSQMTLRKAKFILNKISPEKFDVLTRELLELGIVEDEEIHRGTIDLIFEKAINEAKFCTMYSNLCKHIFEFEKSKKEEKKKQTIENLSEEELAAYQALDKKGAEAFDQEHQLKINFRSLLLSTCQKEYEKIKFESFDQVPENLTGQERTDFEEKQYIERKKVFGLIKFIGELFKQGMLVERVIHGILISLMGELQKPIELKLECFCKLLSIVGRPISQNTQAADYLNSYCARMTQLIDSPQLSQRIKFLMQNVLDLKENSWIPKVDESAKTMKEVENSINKGSSSNDNDRKNMTGVKKNSTNTNMFQMAFNTNSFTKNQPPKNNQPPPMMYNKNPAMNKPPAGFMNNGMMNNNNKYPQQGQYNNFNNMNNMNNKYNQNNNNQNNNSSVPLSSNTSSSNSNTSGWDKIKTMIDDNVNEYLSLQDPAEFYECIKLDVPSADLYTNVISHILTRATEKPQTEKLLVQLLLALIQEFSIFTEQHFKSGYEMFCGSLVDLFEDRPAAYKTVASTMYQFYTLNLLPLSSVAQSLASQVDNCGPTISKIIFEFILQFKDMKKAASVFTDNKIKFSSFFSNTAEKEITSSTTNYNKELDSFLGAIQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1I2</accession>
    <name>A0A8J4Q1I2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002297</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SVP26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000062">
        <property type="protein sequence ID" value="KAF2076392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1I2"/>
    <dbReference type="OrthoDB" id="15490at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097020">
        <property type="term" value="F:COPII receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007277">
        <property type="entry name" value="Svp26/Tex261"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13144:SF0">
        <property type="entry name" value="PROTEIN TEX261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13144">
        <property type="entry name" value="TEX261 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04148">
        <property type="entry name" value="Erv26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2076392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="24026" checksum="3CA3E93FAAD3DB09" modified="2022-05-25" version="1">MFFTVLIYLSYLLYFIFCIVCLACGLYYLSELIEEQAYLAKRFIKYTICFIIGAIFLLWIFEDLSSSCLFLSLLCHCAYLTLLPEFPFVTLTSPKFIVSIVTFVISHFSWFFYFRENWFPFEQIISIFTIFVWLVPLILFISLAASDNSLPRSNISSSSSSSSRIIGVDSDFTSSKRSRVSVLKSGISWVNQKIQELTGSKSANKHYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2N6</accession>
    <name>A0A8J4Q2N6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carbohydrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000981</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000021">
        <property type="protein sequence ID" value="KAF2077734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2N6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035182985" description="Carbohydrate binding domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="147" mass="17218" checksum="DC5276EE959660D8" modified="2022-05-25" version="1">MMLKIFILIILIISLISNTNAKFYVKKRDLDNTEIQVHTTRYESDDNVEEGWRVVTKKVDASDDGIYFNTAHNDRGYTLCYKTNILKDCFMVYPANDPTIVFYPNNIQIDGLYSFAFSPSMVYDFSTDEHHNWNTHNTPYCLNDDVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2W0</accession>
    <name>A0A8J4Q2W0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SAM-dependent MTase RsmB/NOP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_001574</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000038">
        <property type="protein sequence ID" value="KAF2077127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2W0"/>
    <dbReference type="OrthoDB" id="435282at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1170">
        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049561">
        <property type="entry name" value="NSUN5_7_fdxn-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048889">
        <property type="entry name" value="NSUN5_RCM1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF4">
        <property type="entry name" value="28S RRNA (CYTOSINE-C(5))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21148">
        <property type="entry name" value="NSUN5_fdxn-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21153">
        <property type="entry name" value="NSUN5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="7">
        <location>
            <begin position="130"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="351"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="410"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="539"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="371"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="415"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="540"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="561"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="595"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="617"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="461"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="240"/>
            <end position="246"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2077127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="626" mass="70420" checksum="448DBDBF7DF4AD71" modified="2022-05-25" version="1">MCDFYQQAANVIDQLIQKKGSIKGLTYSGQSNNTKTSKTTYALVCETLKYKDVVDEIIGMVPELEKEKSNVKYSLMIVMIYELLFSSKQTIKGGGIAKKTIMQYKTQMVSSLARMKIKKRVADSIDLLPEHIRNPITLPRYVRINSLFNGCMESAVQDAINTFVAEGYDKIDKPVQANNIGEKEFYQDVDFPEILVFNANVDLHDHKMLLDGHIILQDKASCMPSYILSPPPGSVVIDSCSAPGNKTSLLSAQMKNKGQIFAIEKDKKRMGTLVKLTTRSQCKNIEAINDSFLNLRHDDPKYKDVEYILCDPSCSGSGIVNRLDYLLPTKYSTDTNENEDIDDIIKDISNSSNSKRQTESTTATTTTSTTDNEKNKKEMKKDKKRKLKEMKAAEKNGKKGNNNKESLAAAAANAEKLKEEQEEKEKKEEEERVQLLADFQLSIIRHAFGFPSIKRVIYSTCSIHQVENEDVVQRALEELNKDKETWRLVNILPHWNHSRGYSIYKNSEYSIRMTPSIDHTIGFFVALFERIDTTVKAKPLPIPISQPKPTKTPTPAPASAPTPSTTSTTTSQQQKPVLFKSEPTKPSNSTKKKLGNNNNNNNNNNKNNTKGESPMKSNKKKIKLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2Y8</accession>
    <name>A0A8J4Q2Y8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peptidase M8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_000857</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000018">
        <property type="protein sequence ID" value="KAF2077812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2Y8"/>
    <dbReference type="OrthoDB" id="527990at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.132.10:FF:000001">
        <property type="entry name" value="leishmanolysin-like peptidase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.132.10">
        <property type="entry name" value="Leishmanolysin , domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.55.10">
        <property type="entry name" value="Leishmanolysin domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001577">
        <property type="entry name" value="Peptidase_M8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10942">
        <property type="entry name" value="LEISHMANOLYSIN-LIKE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10942:SF0">
        <property type="entry name" value="LEISHMANOLYSIN-LIKE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01457">
        <property type="entry name" value="Peptidase_M8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035203304" description="Peptidase M8" evidence="11">
        <location>
            <begin position="20"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="659"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="610"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="617"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="300"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="385"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601577-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601577-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2077812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="679" mass="74795" checksum="31C483942941957F" modified="2022-05-25" version="1">MNKVIIALFLVSLIYCVSSTSNTNNIIDNIIERNYLSFTKHVDNLKASGELMSNADVDHQNSVLVNGHLPSYNCQHDHIIKSKKIVTPPQPQQTNSSRSRESLAQEVQQTQAVGSIRINFDTTFLYNKVDTRACYQANQQIVFGKGGADCRTSPGTNCVYTCNANDILNNSLANLIENVILPTIREVFSTLLSVNRVDGVLKLTDTYSDRECDDYYRIPDSYFTTGIANTDMLMFITARPTPSTSTIAYALACVFPIYSTNFYGRPVAAGINFNPLYFIPLLNNPNQFLFKEYIRVGIHEATHAFGFSKTFYDSFLDSNTGQKYTYTPSYSFTDSAPTPSGSVSVTRSGIRTPKVVSFVKDHYNCATLQHQLLEDAGGQGTAGSHWEKRTVGEEYMLGYVQPVFPITGLTLSFLEDSGWYDIDFSYAEPLMWGKKLGCNWFAGCTASSWSDTGYFASAQTESCTPTRVGKGTTRLFSYNSNLPAQYQHFSDPRIGGADMAADYCPFSDVTPNTKNAYCVDKGAQANAVTSIFEYYGSDSRCFEYVESSVVKQACWKYQCNANKQLQIQVNDTWITCATPGQISVAQGISVQCPSNFYICDVVSKPIPGLKNETLTIPPETQAPSTTTSTSTGSDSPTPSPSSTTSSTTSPDIDNSGSTLSLSFTLMLAPILSFVWLLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3J7</accession>
    <name>A0A8J4Q3J7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000636</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000012">
        <property type="protein sequence ID" value="KAF2078085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3J7"/>
    <dbReference type="OrthoDB" id="19645at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="86" mass="9666" checksum="5D3EAA48015D65C2" modified="2022-05-25" version="1">MFRLTKIISQLTVRGESRRVPSIKFPDRRAPKTASKPSTPSPQPTQASAPSSNKNKNYTYVDNTQIPKRLQMRKEEMDLISSGGAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3N4</accession>
    <name>A0A8J4Q3N4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein tyrosine phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000025">
        <property type="protein sequence ID" value="KAF2077577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3N4"/>
    <dbReference type="OrthoDB" id="17139at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026893">
        <property type="entry name" value="Tyr/Ser_Pase_IphP-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF1">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13350">
        <property type="entry name" value="Y_phosphatase3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="441"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="135"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="483" mass="55300" checksum="1075B9854589E060" modified="2022-05-25" version="1">MSTPTFEEIDQEQIQGPSILKLNDGNCIKLESVSNFRSLGGFPIGGSDGQLVVRPGVICRSASPTNATFSDEFWLLEVLNVKTLVDLRTSWENKTISPVRKFEDNFIQYSIKKEEASTSPKSGSRSPNSDKHNGNTLNTSNNSNKLNTSNNNNTLNTSNSNNNNHSNTLNTSNSNNNGYYIQPPAIFIQSPEGKQKEMEEQRNGILWRIYNRNLTPDELTKLRSHSSGALRKRYCIPLINSKFFLEGVYHSAPENTKLKCTAARYLLFNDNIGAYMLMDHLNNLGIIEMYKLTLMYTQEEILTIMRIFKNKENYPIMYFCSLGKDRTGMTTAILLSCLGVPRDIIIEDYAKSELNIAPFMEQIRRYFTRVGLSKEEFVRSHRETMAGLLTWIDEAYGSMDRYLECIGFSLEEQQELRDNLIVTKQEYDNFVNEKVNSPNLQKKHDDYIQAQKSKKPPPKLLNSPRFWRKLSFSKDLTLSPTQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q443</accession>
    <name>A0A8J4Q443_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_000294</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000005">
        <property type="protein sequence ID" value="KAF2078427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q443"/>
    <dbReference type="OrthoDB" id="20505at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="548"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="62"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="145"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="253"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="446"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="509"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="79"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="112"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="176"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="253"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="287"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="344"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="376"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="446"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="459"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="469"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="577"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2078427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="873" mass="97570" checksum="7C6F68DB67BA95BE" modified="2022-05-25" version="1">MGSQVSKDHPSSQNFVGQKLSGKKSKNININNKNTHINSDATYSNNDTEFKKYRRYTVHYKSNNSNNLSNITPADLIHNNNSNNSNSSNSNNTSSNITVGGGGVSKSAGDISSSTAPTTTTHSNSNTPPLEQNIQLSSSFDTSTYFHHQQQLHQKHQHHQQQQQQSSSSKKKPRTKSVTTSTNSLDNSEAVQLSNSFNSNISLSSSVNTTPSAFNSPSITPPDSFSFGGSGSSNSNNSHYFSRTRSRSLNLTSLSVNNNNNNSVGNNNNNNNNSHNNIPLHPLSALNNNNSNSNSTSNKPTKIEGRKTKSSTALFKSLTSSLKRTKKDKEKDKQQQQSTKKRSSSTSTPPSELNLSSSSTTTTPPILEEQGKKRSSSITLENNQQQPTPVDNNQQQLNSDNNSIIDIDLEEEEEYKINQTSIEDLSKQLQQDSFFIKENSNNIEVESESLSSSIVINKKPPPPLPPKTFKPTTQQQQQQETMNPNTPPFLSQSQQFQLQQQSPYSSGFSFGRKSGVLPPSEPTYPSESSVSSKRNSVELRYLEDTHSYQLLEKLGSGTFSDVFLCMHKETQKQYAAKIIDKSLVQMIAAHTEMEIATEVNILKASFHPNIISIIDHFETENYYYIITELLNGGELLYQLEKNHHATAPDTQNYTEEHAKKIIKQVVQAVSFLHKNNIVHRDLKPENILFRDKSHCSSLKIIDFGLASQLKASTHSLIDVCGTPEFQAPEMVKREGYSFPVDVWATGVILYILLCGQPPFQGKNSMAIMSLIIKSELNFDLPGWESISDAAKDLIKKMMNPDPATRITCEQILEHPWILESKMDSPQLGSFHKQLRRYNSQRYFKATGDTLLKSQRFGIYSPEKELYKRRSIQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q6F8</accession>
    <name>A0A8J4Q6F8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Rab GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_003769</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000122">
        <property type="protein sequence ID" value="KAF2074936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q6F8"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01861">
        <property type="entry name" value="Rab6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000229">
        <property type="entry name" value="Probable Ras-related protein Rab-6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2074936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="23097" checksum="F8FCB88EBC196FBA" modified="2022-05-25" version="1">MESLSKYKLVFLGDQSVGKTSIITRFMYDTFDITYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSYIRDSSVAIVVYDITNRNSFVNTIKWIDDVRNERGNNVVIMLVGNKTDLADKRQVSMEEGEAKAKEYDIMFTETSAKAGFNIKALFRKVASALPGIDQNSVPKNDGILSEIILSTDNKTPQTEQGSMCRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q7U5</accession>
    <name>A0A8J4Q7U5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Importin subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_002496</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the importin alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000069">
        <property type="protein sequence ID" value="KAF2076196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q7U5"/>
    <dbReference type="OrthoDB" id="16542at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024931">
        <property type="entry name" value="Importin_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005673">
        <property type="entry name" value="Importin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2076196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="448" mass="50491" checksum="CE527621CFB7E46C" modified="2022-05-25" version="1">MEQIIELINSDSAKSMLIGTESLRKLLSPDRGVIAQKVIDVGLVPRLLSFLDPLVPDSLQFEASWCLGNIVSGKSEHSHHVMQHGALFSFLRLLSLPQIDSGVKEQCLWILGNIASDSREYRDEILHSGLDKILGLFELPIEKMKISMLRNLVWTLSCLSKHKPHPPLHMMVPVLSWMAHTITCRDTEVLIDSSWVLSWLTVTDEGAQCAIDTGIAKRVVELMTYYEPILVPALRTVGNLASGTDQQTQVILQLGALPIIRTLMSHNKPSIRKECFWILSNIAGGTPEHIQQLIYHDFFPHLLKVLVSEAEDKAHKKEIIHVLNNASSGGSYLQILYFVHTGFFQALQHYIQFNYISDPYTTKIALEALETLLDLGVSLGKEIQEENIFIEICVKLDFQKLLELVESDDNTLNSQCQQILTKFFSNGTTITTQKKSIEQLIEKLSIQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPU9</accession>
    <name>A0A8J4UPU9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008927</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the API5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000600">
        <property type="protein sequence ID" value="KAF2069751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UPU9"/>
    <dbReference type="OrthoDB" id="19224at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008383">
        <property type="entry name" value="API5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12758:SF19">
        <property type="entry name" value="APOPTOSIS INHIBITOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12758">
        <property type="entry name" value="APOPTOSIS INHIBITOR 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05918">
        <property type="entry name" value="API5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="428"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="529"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="428"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="533"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="576"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2069751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="626" mass="70267" checksum="56401EBE2D816216" modified="2022-05-25" version="1">METETPTSTTTTTTNTATSMDTSTPTVAESKAVRNDQYINTFYEIANKLDASPSFADDDKLYLQIIDLSQKTKQTKQLSSQFLAKYFKRFPSLQEKAINSLIDLFESDDLPVRINALKAIPTICRDNPEHIVRLADILGQLLNTDSKIESENIKSSLISLYKLHSVNTINSILAFLESEESDPNQHLMLSFLKEHLLPLIRQEYAKSTVETQTYFRDRIIKILPTAKEEETTVLLELLNCFPQYKIQDLIATIDLFIPQIESKPINDNIKKIFHFMRSLIHKSNQTNENISCKIFDLYLNKLIPATNELDAGVRAEMYSYLAQISPIMNSTDASLIIEPIFNYFKTIVPTSGEEIDLPYSIIESLLYCFANFGYKSMGSLRKLCGYKTITGQPSDMNAEVDPTKFADLLARFRFLDEKSRETALKAQKAIQAKPAKQAKSSPSTTTTTTTTTTPTSPTTSTTPTTTPAAVAVTDEKAIRQLAKKTLTATKNINALIAQLSKNPPVLSTSSIQLSSSITRINLKKFEKSLNKPNQNNNNQNNNNQNNQKRNNQNQNNNTNNNNNNSGNSPKVGGSGPQRTNKQTRYQPYVVPGRKQQQEDVDSAHLSIVTEKHKKPSNFKGRGNKNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4USF8</accession>
    <name>A0A8J4USF8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cystathionine gamma-lyase</fullName>
            <ecNumber evidence="3">4.4.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Gamma-cystathionase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_005125</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.1" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000196">
        <property type="protein sequence ID" value="KAF2073576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4USF8"/>
    <dbReference type="OrthoDB" id="3512640at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000046">
        <property type="entry name" value="Cystathionine gamma-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000008">
        <property type="entry name" value="Cystathionine gamma-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808:SF80">
        <property type="entry name" value="CYSTATHIONINE GAMMA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="TRANS-SULFURATION ENZYME FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2073576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="410" mass="45009" checksum="8DBBC38A711D2F6D" modified="2022-05-25" version="1">MQTDTEKATTTTPNKLGFDTLVTKKVEVRGPVSPLCTPIYQTSTFVLKDTRHGASLCSHQELKQDESPYLYSRWSNPTNDVLERLITTLEGGYSTYVTSSGMAAISTVLLSFLKAGDHVIITNNVYGGTHEFATDILSRYGVEVSWVDQNDVANYRNAIKPNTKLMYGETPANPTMVLLDLQAFGALGKERGILTVVDSTFASPYNQRPIEFGVDVVVHSATKYYGGHSDLIAGAIIVANETLDKSLAHQFRLFGGALAPNIAFLLHRGIKTLALRMERHNNNALQLARYLESHPKIKKVHYPGLESHPQHELAKKQMRGFGGMIGMEIHGGLEAGRYLVENLKVFTLAVSLGGVESLIEQASTMTHGMLTAKDREKAGIPDGFLRLSVGCEDVNDLIQDLETTLSEINI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UV15</accession>
    <name>A0A8J4UV15_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_010484</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001144">
        <property type="protein sequence ID" value="KAF2068190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UV15"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF184">
        <property type="entry name" value="GTP-BINDING PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="4">
        <location>
            <begin position="19"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="218"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="333" mass="38096" checksum="7C1272DCE661FDBF" modified="2022-05-25" version="1">MEAMDTTSTPTTPLQERVILCIGKTGNGRSTICNVLSETMDFKESPNSVSETKEHKKQEYTIDGIKYVIVDCIGFGDTRLTAQQVFFLIADACFNVRDTGLHQILFVTTGRFTEEEQEVFNLLRSTIFNPEIVKYTTIVKTRYPKFRNPQACQEDLDKILAEGTDEIRSIIQSCNKFIHVNNMCEDEDPVLKARKDSRTLLATHLYNNCREIYKPKELDQLNDKIVNYMTEAQKSKARIDSLQQELKDTTNKNNSTLVELHPELQKIQNEIKAGQESYKKAQEDAARQTAEIIIAREEAQRARDKAKRATEHEIVLIQARRYGGEGRGFCSIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVU5</accession>
    <name>A0A8J4UVU5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Steroid dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_001809</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000045">
        <property type="protein sequence ID" value="KAF2076906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVU5"/>
    <dbReference type="OrthoDB" id="5545019at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05356">
        <property type="entry name" value="17beta-HSD1_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000137">
        <property type="entry name" value="Hydroxysteroid (17-beta) dehydrogenase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051019">
        <property type="entry name" value="VLCFA-Steroid_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43899">
        <property type="entry name" value="RH59310P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43899:SF13">
        <property type="entry name" value="RH59310P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000126">
        <property type="entry name" value="11-beta-HSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2076906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="34182" checksum="A041BD15F29CF628" modified="2022-05-25" version="1">MLDTISWAVGFFIITFSLFRVARFFYNFFIRPSVNLKKYGQWAVVTGATDGIGRAYCHEFARKGLNICLISRSLDKLKKEAEEIERDFKVQTKVIAFDFNTNDNEKLSNLYKSFADLDIGVLVNNVGISYEHPMYLEELQVNSIESMINLNIRAVTLLTKFVLPSMVEKKKGAIINLASISGVNTCPLLSVYAGTKAFVEKFSVSLHYEYASKGVFIQSVTPALVCSNMSKIRRSSFFTPLPKAFARSAVATIGHDRNTTGYWTHEMQAFAIRTLPVFIIDKVMFGLHFSQRKRALNKKKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UW85</accession>
    <name>A0A8J4UW85_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Clu domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_001421</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000033">
        <property type="protein sequence ID" value="KAF2077296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1667894at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033646">
        <property type="entry name" value="CLU-central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025697">
        <property type="entry name" value="CLU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027523">
        <property type="entry name" value="CLU_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601:SF15">
        <property type="entry name" value="CLU DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT EIF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13236">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12807">
        <property type="entry name" value="eIF3_p135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51823">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Clu" evidence="4">
        <location>
            <begin position="379"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="1110"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="129"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="253"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="299"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="377"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1346"/>
            <end position="1366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1378"/>
            <end position="1554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1572"/>
            <end position="1755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1806"/>
            <end position="1863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="134"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="217"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="256"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="309"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="377"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1381"/>
            <end position="1392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1393"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1573"/>
            <end position="1602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1613"/>
            <end position="1637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1655"/>
            <end position="1686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1687"/>
            <end position="1718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1719"/>
            <end position="1738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1813"/>
            <end position="1822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1830"/>
            <end position="1857"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2077296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1903" mass="212656" checksum="995FBD2BA1C80076" modified="2022-05-25" version="1">MADKPRKDLRYHQNSNSTATSDVSSNNVHNDRKLYTSSHESVWYFLKNESYSCISHISEFQSYQQQQETKSVCSYIFDPSSQYTSNTATDTASNSYSSFYSKSGNIRPLDSSYLETNSEYIRDSYENKNHIKDQPSTSSADSAQTTPTQTSTSTTTSADNSNNYSGNLYLIKESIWRNDTNGIVNNASAYIDGSMSYQQAPVSAGSYSSAYTLDSSVGSLSSSAGQQQQQQQRMTINRDPSYNNIMAYLQQPYVDNNNSNSNNMKTSTSSIKSSTSSTSSGSSNSPFFSNYHDNNFIRDSNGGYQQVFSNSNGNGGNESSHYLSNSASSTSSNLSTSNGSTISTNTTNTINNINNISRQGSAIDSLVSLANSISINSNNNNTATANADPTATNKQEDGNTTTNVLPWNESFQQLLDLPIKSLEDERIRNQNITFFSELLAAQASKYVKTLVREVHLNPNLKTIKPYNCGGLAGGLKYSIDNMFIKFALNPYGIYEQQNPTSYFANKAAGHELKSINSLVSCAVPNLHFPLMCILNFRGFRLSVISELPISSNTIVFGSSNAGHMIHDNQTVYKMMVKIAEMLNLKTHLVEQSKSGGGDLFKNTAANKQFSIPLAVDIEGHFGFDGRYYIVDSARLFPPESPAYGITGGFLYRLFRTEFLKRYETQLCADAFSNFNIVNPKQHNDEVEKATDFLVEQVIPNFAKHVLPVLPIAKLNIKEHLHKEGINLRYLGLVLYHVVSTSLQNKHKDELIGKFVIEMLSRILRYIIFSEMRKLNEIEDEPHLYVALTHLNLILSPYLTGNNFIYWTQFISNSLVAKYTSSLHAKPTQELCVTQLIRDSKGFYLYFSNDSNRLRYKLLKKFTQLTGIIISPSYLERLKNNRDNSSIIPPLILSDILEIQITVKHMKVSPSFKAFEQFPEAEQYYLKELEFKSNKLGNDHTQVAYTHVHLADLYSSYSLLVKAEKHLHLSLQIYQSRFGPDDISYQDIKEKLAVLCIKQKKFDEAIKLLKEILDVKKKQLSSDNIDIADTCDNIAWAYQNRGDFQLSLTYYTEALKIKMDKTGNSMSTAKTLNNLGHLFFSNNELVKSLDIFSKVKEIFIQQKGPDHSDVGTAYDNLGMVYSKSREYQLAEKSFKSAIEIRSKQFGKESRLVAITQDHLAQLYVSWDKTKYDDATKLLQSSIHIVDQFPDFYSQAFMRFSLSKVYKAKKEKQQELKLLNESIQIFDLHNIQSDLTPKIRKRIQDLSKSGLSKLISWINNPVVKMPPPVTLINHNMVESQMYGGHMEMKNDNSSFVSKSSSIPKEWQDLFSEAGISNEDMADPKYSEAIREIIEESFKTTATSNRATAKKSVVANSRRSIRARKESAQETIKTLQKDAKNLLSSSSVSSSSSSVCKEQTTANMASNGSPKLQKKQPAIRSIPRPSTAIPPSGGAPGAPAPTSSLSKFKVKSAPPPPMQAPTALAPRPTLSPIMAPITPPAPISPVMEPTPSSYDKSSADMRHKRVELQKPNQKKSATDIFGLFRSEAPKEKEASAPQPELSRANSPARAPIRNNNIVSRSQFADQLNVAPSAAYSLASPTPVPTATVTSTTSTSSSLYPSLSDPVPQPTPSLVFSPTLSTSPYSSSSSMSPYVASDSSPYTSPQLQAHVYYPPPSAPQYYQQQTLASPSPQPSAAYYAPQYYQQQQPLASPPPQPAAAYYAPPPPPPAASGYHAPPPPPAASGYYQPTPQPQIQQQQQAPARTSYFAPPSQNIPPLQAYKPTVNQLYPVVPSISSYDMTSQYLSIEQQQLEEVSRMTQDLSQLQEQAGAIQLDAIEQEQEQEEQEERKEYYDSSEDEGYDDDDDDDGIEYDEDEDDEYYQQEQQQEGIYWPQFNLNLINKEKLIETIKKRIHETKTDVKSVMKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXL6</accession>
    <name>A0A8J4UXL6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007589</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000414">
        <property type="protein sequence ID" value="KAF2071085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXL6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="100" mass="11013" checksum="217923D2652196CE" modified="2022-05-25" version="1" fragment="single">MSTAVEIITPTTTIINNMENNNDNNNNTNKNEVSIDISESSSLDDTYDIISIDESIKEDETPKEDLEVSPPLSPTTLHIATAFLCFLLLSLIGLIVLVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXV7</accession>
    <name>A0A8J4UXV7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase</fullName>
            <ecNumber evidence="10">2.7.8.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_007688</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Functions in the biosynthesis of the anionic phospholipids phosphatidylglycerol and cardiolipin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>a CDP-1,2-diacyl-sn-glycerol + sn-glycerol 3-phosphate = a 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycero-3'-phosphate) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12593"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60110"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Phospholipid metabolism; phosphatidylglycerol biosynthesis; phosphatidylglycerol from CDP-diacylglycerol: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the CDP-alcohol phosphatidyltransferase class-II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.5" evidence="10"/>
    <dbReference type="EMBL" id="AJWJ01000424">
        <property type="protein sequence ID" value="KAF2070993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXV7"/>
    <dbReference type="OrthoDB" id="10250191at2759"/>
    <dbReference type="UniPathway" id="UPA00084">
        <property type="reaction ID" value="UER00503"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008444">
        <property type="term" value="F:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09135">
        <property type="entry name" value="PLDc_PGS1_euk_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09137">
        <property type="entry name" value="PLDc_PGS1_euk_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016270">
        <property type="entry name" value="PGS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12586:SF1">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12586">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--SERINE O-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="12">
        <location>
            <begin position="223"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="29"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2070993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="556" mass="62616" checksum="0BA4A92F322B9FAF" modified="2022-05-25" version="1">MIKLRLLPWVQPQRLFAALMIIGGSKSTATGTTSTLNIPKKSTLTSRPIGNYSREYSTSSSSTSKSSTTSSSSSSKPVKSIFNDTYLNDVFWNLSSQGPAFAINPNNVEFIQEPSDFYNQLINGIKTAKKRITFASLYLGIGKLENEMVNEMILACEKNADLKVHVLLDGLRGTRLSPEKESSATLLHRLKSKFPERVTISLYHTPDLNGILKKVLPPRVNETIGVNHLKTYVFDNDLLLSGANLSKDYFTNRQDRYSWVKQTESVSSYFDNIVSIIGSISLRLNEKGEVYLPPGTVDPVTESKEFKSVAYKKLSTLLQPNTFYSATGGLQAISSPYDINDIAKKEDITWIFPTIQMGPFNIRQDEVVTSYIFDSVPSQSKFYITSPYFNLTNNYLQLILRGKPMLDIITCSPQANGFYGSKGISGAVPDCYSLIEKGFLEKVVNSGNQDRIKVEEYIRDKWTYHAKGLWITAQNQEDPSITLIGSPNFGSRSVEKDLEAQIILITQNKSLQQKMKSEKEYLWKDTQDANLKTFEQPDRKVSIWVRMLVYVFGSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXZ5</accession>
    <name>A0A8J4UXZ5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GH18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_006897</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the degradation of asparagine-linked glycoproteins. May hydrolyze of N-acetyl-beta-D-glucosamine (1-4)N-acetylglucosamine chitobiose core from the reducing end of the bond.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000338">
        <property type="protein sequence ID" value="KAF2071790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXZ5"/>
    <dbReference type="OrthoDB" id="16630at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009313">
        <property type="term" value="P:oligosaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000250">
        <property type="entry name" value="Probable di-N-acetylchitobiase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051887">
        <property type="entry name" value="GH18_Domain-Containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46290">
        <property type="entry name" value="DI-N-ACETYLCHITOBIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46290:SF1">
        <property type="entry name" value="DI-N-ACETYLCHITOBIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0458">Lysosome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035187288" description="GH18 domain-containing protein" evidence="9">
        <location>
            <begin position="20"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="10">
        <location>
            <begin position="1"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2071790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="385" mass="43651" checksum="0E3614171A675581" modified="2022-05-25" version="1">MKYLTLLVFLVATLSFAASLSSSSVVDDIKPQKIQCPCSNKTLCQPINPGNERKELFIFSIVQGNYKYYDWSQITTYAVFYPDPVGEALCVAHENNARLVFQASYPLLQLTNSTYKKEWIKQQIDLVENNYADGINIDFEASINDTQNELSALYTALVAEVSLAMKKVNPNYQVTVDVPYAPYCTFGRCYDYLGLSQAADFLVLMFYDLFGSYPANPCIAGPNSPLLLVPEGLENFTLTGVTKDKLVMAIPWYGYTYKCQNATNLNLEDCSLEQNVNGQVTCPHPMVSMDYGAIVNMIQDPKNKTSGPLWSDKYSSVYLNYVDQQETIHQVWYDDEETIELKVNIAKKYSIRGISSWNADQLDYSDKISSSLMWDSFKPFFEQQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZ81</accession>
    <name>A0A8J4UZ81_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004466</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000158">
        <property type="protein sequence ID" value="KAF2074220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZ81"/>
    <dbReference type="OrthoDB" id="202203at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004174">
        <property type="term" value="F:electron-transferring-flavoprotein dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735:SF6">
        <property type="entry name" value="FAD_NAD(P)-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="2">
        <location>
            <begin position="53"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="397" mass="45053" checksum="2C727418AF50D517" modified="2022-05-25" version="1">MIFSFILYIIFFFYEYGLSFAFSLLKILLICQSWFNILVSKVTNKTPIEDRKRVVIIGGGFSGSIVAQKLENDYQVTLIDTKDFFEFTPSILRTIVEPTHVRKIQVFHSHYLKNTNVVQKEVMGVAEREVVLDDRTIGFDYLVINSGSSYNSPFKESSVVASARANTLRENYYHIRKLKKILIIGGGIVGVELAAEIVSSFKGKEVTIVHSQSKLMNRFPKKAIKYAEQYLLDRGVRIIYNERVIAHRGNVFVTDQASEIIADQAFLCTGIAPNSGFIRNSYPDALSEFGYIKANEFLQMAGSTYYRNIFVSGDVLDVREEKLAQTAECTASIVVNNINALETRSEHKFKSYRPFSKPVLVSLGKYCGIFVYKNMAVTGFLPAMLKEAVEYKTMVRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZ91</accession>
    <name>A0A8J4UZ91_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAD synthase</fullName>
            <ecNumber evidence="2">2.7.7.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">FAD pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">FMN adenylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_006007</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>FMN + ATP + H(+) = FAD + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17237"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.7.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.2" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000258">
        <property type="protein sequence ID" value="KAF2072673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZ91"/>
    <dbReference type="OrthoDB" id="270728at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23948">
        <property type="entry name" value="FAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23293:SF9">
        <property type="entry name" value="FAD SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23293">
        <property type="entry name" value="FAD SYNTHETASE-RELATED FMN ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoadenosine phosphosulphate reductase" evidence="13">
        <location>
            <begin position="67"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2072673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="264" mass="30538" checksum="A0D663CF9CF9C136" modified="2022-05-25" version="1">MNSNNVVFDISTFINHPTVTLTNSSSSNSCKYNREYTESETKELLDRISHSLCIIEEAFQKFTFKELALSFNGGKDCVVLLHLINYVILKLHKDNNNNIENKTISTDKLNTIYFTSKDSFHQVNEFTEKCSKIYNLQTVNISSGIKEGLQEVVSNNNIKAIFVGTRHTDPNCSHLEKFSYTDPGWPHFLRVNPILEWNYTNIWHFIQLFNVPYCELYDQGFTSIGHINDTIPNPDLRVDNTDQYLPAYCLKDCTSERNGRLRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZF1</accession>
    <name>A0A8J4UZF1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005770</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000239">
        <property type="protein sequence ID" value="KAF2072913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZF1"/>
    <dbReference type="OrthoDB" id="15863at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="16152" checksum="CF6BE851C9F65F49" modified="2022-05-25" version="1">MHHDEYVQLTRSQYWFIVAASLLGTFGVMGWCQMPFDIILGFLNIILMIIGIRGLHKKKKLFLRLFLWGMIAIAFLHIVNLAVIIIVDHKYKKHSRFGFAPDIIIVVIRIVYSLALSFFTGFIKNTIDYKKPPSQLNRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0A5</accession>
    <name>A0A8J4V0A5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">COMM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004529</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Scaffold protein in the commander complex that is essential for endosomal recycling of transmembrane cargos; the commander complex is composed of the CCC subcomplex and the retriever subcomplex.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000162">
        <property type="protein sequence ID" value="KAF2074158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0A5"/>
    <dbReference type="OrthoDB" id="76101at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051059">
        <property type="term" value="F:NF-kappaB binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033209">
        <property type="term" value="P:tumor necrosis factor-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017920">
        <property type="entry name" value="COMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047155">
        <property type="entry name" value="COMMD4/6/7/8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16231">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 4-8 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16231:SF2">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07258">
        <property type="entry name" value="COMM_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21672">
        <property type="entry name" value="COMM_HN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51269">
        <property type="entry name" value="COMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COMM" evidence="2">
        <location>
            <begin position="139"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00093300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="210" mass="23887" checksum="B87FEFB117DD7973" modified="2022-05-25" version="1">MVVYKFLGNSNNTGESSDQQQVINEVGYLSKLSDEALVQLIEILFEFLIVQKAEVLMNNISNYSSQHSINENTLSNIFRSTLYVIKGCIKNNVTPSHLKEDLISMKLGDEQAEIISSRYKLNFIGLSRSFVGNSLSVNQVLDMQWRFGVTTATSENTSSKQQSSTFLQLKLVLDKGDNIKEDVHMELTLPQFYQFLKEMEKAKSSLEYFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0H0</accession>
    <name>A0A8J4V0H0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Galactokinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_009487</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GHMP kinase family. GalK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000721">
        <property type="protein sequence ID" value="KAF2069197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0H0"/>
    <dbReference type="OrthoDB" id="187738at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004335">
        <property type="term" value="F:galactokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000705">
        <property type="entry name" value="Galactokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019741">
        <property type="entry name" value="Galactokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019539">
        <property type="entry name" value="GalKase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006206">
        <property type="entry name" value="Mevalonate/galactokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00131">
        <property type="entry name" value="gal_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10457:SF7">
        <property type="entry name" value="GALACTOKINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10457">
        <property type="entry name" value="MEVALONATE KINASE/GALACTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10509">
        <property type="entry name" value="GalKase_gal_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000530">
        <property type="entry name" value="Galactokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00473">
        <property type="entry name" value="GALCTOKINASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00106">
        <property type="entry name" value="GALACTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Galactokinase N-terminal" evidence="6">
        <location>
            <begin position="35"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="4">
        <location>
            <begin position="121"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="5">
        <location>
            <begin position="373"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2069197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="476" mass="53266" checksum="FD8AE72834BA402D" modified="2022-05-25" version="1">MDIFNPLLPSVVDNLAEVYENVESNQERYQILESKFKQLYNDKPLFYFRAPGRVNLIGEHVDYSGYPVLPFALQQDTIVAVSINNNNNLNIQNVNEKYTPKVNLNVDLEIEIDMANHHWTNYVLAAWKGVKNLGQTNSPLKALNLLYSGNVPTGSGVSSSSALVCVSTLAFTYINNLVFTKEELVDLSIKSERFVGIESGGMDQTISYLGEMNVAKLIEFQPLKTYDVTLPKSVSFIICNSLVESNKVITGGIYYNLRVVECRLAAVLLAHSLGLKWETVRKLKDVQYQSNLSLEQLIQKTEEILHKSAYTRQEVADILEMSLETLEKTYFPKGIKVAAQSFELYKRATHVFSETLRVYQFSNRCQNLVSNASHDLGALMNDSHFSCSKMFECSCPELDQLTTICREFALGSRLTGAGWGGATISIVESSKIESFLKNVNEKYYSQFDKSKIPVDSSHIIISSPSKGASIIPSSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1M9</accession>
    <name>A0A8J4V1M9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010426</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001104">
        <property type="protein sequence ID" value="KAF2068249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1M9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="99" mass="11262" checksum="75175930AD6531F9" modified="2022-05-25" version="1">MLSVPSLPFPLSLITDNVQYLKVQQLEEIAKTGQPSIEYLWIISLSRNYTPHDIPNTVKELVLGSCSLDDMRITPTFTSPIHINDLKVYNGEEIDNTWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1W1</accession>
    <name>A0A8J4V1W1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cullin family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_001121</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Probable core component of cullin-based SCF-like E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9 10">Belongs to the cullin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000025">
        <property type="protein sequence ID" value="KAF2077580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1W1"/>
    <dbReference type="OrthoDB" id="27073at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031461">
        <property type="term" value="C:cullin-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000091">
        <property type="entry name" value="Cullin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000001">
        <property type="entry name" value="Cullin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000006">
        <property type="entry name" value="Cullin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000004">
        <property type="entry name" value="Cullin 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000002">
        <property type="entry name" value="cullin-3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016157">
        <property type="entry name" value="Cullin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10557">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01256">
        <property type="entry name" value="CULLIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cullin family profile" evidence="11">
        <location>
            <begin position="376"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00056946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2077580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="733" mass="85904" checksum="097868442463FF8A" modified="2022-05-25" version="1">MATKPGTGRILLKINHSTQDPEFSRRTWKLIKSAIREIHLQNASCLSFEELYRNAYNMVLHKHGEILYNKLKKMVDKHLKVVAQKVSSAVDEYFLSELNKSWIDHKTSMLMIRDILMYMDRTYVEQNKVHSVFELGLILFRDNVVHAPQIKDRLLKTLLSLVQKERAGEVIDRILIKNITQMLIDLGVNSKNVYIEDFEKPLLLTTASYYQGESQAFITTCSCPDYMKKVEICLKEELERVSHYLDNSTESKLREVCEKQLISNHMRTLIDMENSGLISMLKEDKIEDLKRMYNLFNRVSDGLNLMKEVISTYVKEIGKEIVMDEEKTKEQGTFFQSLLDLKDKYDHLLQFAFYNDKQFIHSIHQAFEHFINLNPKSPEYISLFIDEKLKKGLKGVSEEEVDIILDKILMLFRFIQEKDVFEKYYKQHLAKRLLLGRSVSDDAERNMISKLKTECGYQFTCKLEGMFTDMRLSVDTMTGFKEYIQKLQKPLPIDLNVHVLTTGFWPTQNTSNCILPRDILLCCETFKSYYLSNHIGRLLLWQTNMGTSEVKANFPSKTHELQVSSYQMVILLLFNDSPKLTFKEISDQTGIPLVDLKRNLQALINPKNKILDRESTNKTIEDSDVFNFNTKFKSKLYRVKVMAVVQKETPVEAQETREKVEEDRKHQIEASIVRIMKARKTMDHSNLVSEVLKQLQSRFVPNPIIVKKRIESLIEREYLERSKQDRKIYNYLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2B3</accession>
    <name>A0A8J4V2B3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_010081</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000915">
        <property type="protein sequence ID" value="KAF2068594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2B3"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052591">
        <property type="entry name" value="CML21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23064">
        <property type="entry name" value="TROPONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="12"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="49"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="102"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="148"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="221"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="581"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="297"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="615"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="796"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="862"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="964"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="195"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="590"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1056" mass="120637" checksum="90043F7C2B63519C" modified="2022-05-25" version="1">MIEGDKMIEGDNQDTYLIKLFNEFDVEGTGYLDIDQLQHTVSKITGKEVDHEELTTLMEKFDLDHDGKVSLDEFLKGCSTNTVLDDEGNQHYISSYSVGGEDYEKHLKFIFSLFDKDGDGLVSIDQLRQMLEDIKVKSGGDDMVDDENDDDDIEYIINTLSADGNETISFDSFMKAIEQINSPAIPSPDVEEESTSSFQPSKYINNNNVHHEEVMSDNELENNHHNSSFEDANGGHSRPESPSIESHQHQQQQKKSKRGRVSSWVAPMSIVNMIKRRQDLFPKEENEVFDSSHEKLVDQYKKRADKLRTENEHLGSKINMVEGQLREFKESHEKLSEDNAQLKETITIGKKTLIENQKLNSHNKKLEDYLESLKQQLEQAEEQKLDLLKSFNKLKSEKEATANDLVEKEQRLGELEKKCRQLEENADNNNISNLNSTFQSIPLAEVEKLQQENEQLRVTLTLENEKAQENIKLLSEQYQLLKKQSKDDRQLLESSNQIIHKLRTSLEELQSNGHGFDPNYKLSLLSELEQQVVTRLGSTPLPDSPIAIKSLVGSNDEQDSTSTTTTTTTVVSKTIPIKSTAGKSSQNEILTDDGDLSFNNIDDDEDSGSEYKIKYEEQVEKVKTLTADLESLESSTQQQISALNNQLLEQKKNTNQQIIVLQQSNQANTDQLTLSIDNLKQQLSDKENTIHDYQLKLDQLAQKSIVEKQEILENVEQLEKEIKSLNSQLESQNSSDKEMIQSLNVSIASLQAKQQETSLELEHSITENKKLKMHQEAFAKSIDTPSTQSDAINIMAGQTQSELSNLKARIEELSTKNHHLVHENTQLKQSLTPTSSPVTSSQDINASSLMESSNISNNNNNVESLQYEIKTLNNVKEQLEKDLQQYQSLAKSSSLPSNNNLIDEMKRDNEKVMEKFRVSEYEKLMIQEENKSLLDKSNTLSERIEMMKIEFETKLNQIQINDKKSKLIKENDDLKSRLKKYESNDEHHIDIIELEDETKTMKAKIDDLSSKLQNERNLTKYLQGQLDKMEKSSDQDQTPLISNSNYKKNRDCCLIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3A3</accession>
    <name>A0A8J4V3A3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_006310</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the proteasome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000285">
        <property type="protein sequence ID" value="KAF2072392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3A3"/>
    <dbReference type="OrthoDB" id="268428at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03758">
        <property type="entry name" value="proteasome_beta_type_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035206">
        <property type="entry name" value="Proteasome_beta2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF2">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2072392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="192" mass="21333" checksum="127ED13026CC4874" modified="2022-05-25" version="1">METLLAFIVNDGVLCLADAGITRSIVKMKEDEDKIMVLEGNKLLLSAGEPGDRAQFCEYIAKNLKLYNLRHGYSLSTPACANFVRSELAASIRSNPYQVNLILAGFDNDTGASLFFLDYLGSLQKLDFACHGYASYFLLGLLDRHHKTTLSAAEGVDLMRKCVEELQTRFLVNGKYVLKHISKDGVMVIPFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3U9</accession>
    <name>A0A8J4V3U9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005754</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000238">
        <property type="protein sequence ID" value="KAF2072932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3U9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="324"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="611"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="292"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="324"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="368"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="420"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="443"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="459"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="470"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="558"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="917" mass="101733" checksum="2AD02C233D9A3CEB" modified="2022-05-25" version="1">MDREKQIKLLKEVTISDLKDKLPVLHKKITNPALPNVTVVDPNTISILFVKPIDVAFSNPSEIFTVKLRCLKNQLTVEDVGTIIGSFHSKKHQPIERSKSLPSNIKEQHFTVREMRMVLMKTFKGMNALEADIMARYYQRIICDQCSKDNQKFKSAIVLTNRIRSRIEEDEGLDESFDDEEDEEDENGDHMDIDEKRTVNNNKSNIHEEKSKNAEASDSDTSTPVTRSKQRSIATTVTTTTVNKKDKKKEEKKPKGQEEKIVEEKAKPEEKEKDKNTEDKPTLSTPAPPPVTTATITSPATVKSTKVDKRGTTITIPVAAMTAATAASSTTTTPQDDPAPILPLKRPRNDRVDLTFLNSSRAKKSSGTNNNNPATATPTPTTTISKDTASLPPPPKKLTGGAPPRKVGPMGRISSIPKIIDMKKEPEVKPEPEPTQSQPEPTKSQLEPQLMSDQESKEEENPQQKEKEQPQTQITKTKTPTKTLPNPISTPTSPSTPFALPTPVLPSNSNGNSNSNSTTSFSIPSPTKQPQSQIPHPKKRQKLLLQQQQQKEQEQLEKGQLASSNPSTTTSSNSPLTSSSTAFPSSVSLSPPLSTTSLIPTETLLNAMIGSPNRSLVSSPSSMTTTTSTTSPTPTKSIITMTNSPPTTPSSLSLSNPIKFSSQQQVHLESYKYDESYKQYLLKPNSKAEYRKILSTSAPYLNNNIIDYMMGKFFSSMSLGSTYDNILALNLDTLSNVHKLESPVKIDKYKYIFIPDNIGSHFSLWLIFIEEEGISIFHFDSLTYECTPCNNDLFGSKISDLLNHLPFEADTVNHIYTAQQNDLSNCGVHLVSNYMALLTLCFNSVKQPNYDDICKSIQNLGKTPINRAQILSTLEKDQKDLLLKHSTYVIDKYFSGDLLDSTESLVIENLFLDSLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4M1</accession>
    <name>A0A8J4V4M1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004999</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000189">
        <property type="protein sequence ID" value="KAF2073682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4M1"/>
    <dbReference type="OrthoDB" id="17701at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="108" mass="12397" checksum="6652B44B2071B1F0" modified="2022-05-25" version="1">MDRVQKLYQLLKNIGQTNIKGTNFEPLLKNFEAQSAKAAGQNDTVKNVMDALSPLNDPTRVFAVWMGSFSVFYFVKSIFFGDHPHQHGTYPYMKSKRRTLAFMFEGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V574</accession>
    <name>A0A8J4V574_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_000810</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000017">
        <property type="protein sequence ID" value="KAF2077848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V574"/>
    <dbReference type="OrthoDB" id="9974421at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000057">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006693">
        <property type="entry name" value="AB_hydrolase_lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025483">
        <property type="entry name" value="Lipase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04083">
        <property type="entry name" value="Abhydro_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000862">
        <property type="entry name" value="Steryl_ester_lip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035322190" description="Lipase" evidence="9">
        <location>
            <begin position="18"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Partial AB-hydrolase lipase" evidence="10">
        <location>
            <begin position="35"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="341"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000862-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2077848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="398" mass="45339" checksum="B0477E616CE1D00F" modified="2022-05-25" version="1">MKSPVLVLLLLIQLINANQDKNELDILLESKLNLEELIQSHGYQTEKHYLTTGDGYILTIFRIPWGINGKGSKDRKPVILQHGLLDSSFGWVVNLPHQSLAYILADQGYDVWLMNFRGTTYSLNHTRFDSKKDKEYWQFSFDEMAEYDTPYLIDYVLEYTGASHVGLVGHSQGTLVSFLSFLKFPELAYKVSLFVGLGPVGNLTHMQNPILKGLASLHFDGLLSFLGINQFLPSPKLFRNQFINVCNICNGCCSTVIEMLVGASHRQNSTRVPLIAANDPSGTSMMNMNHFTQMYRSGVLRMYDYGAKENKRHYGTSLPPLYDYALLPNNLNIVLYSADKDILTTPTDVDQLVQKLPHPTLKYWQIVENYSHVDFIWAIDAHQLIYPTIIEQLNEYLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5M9</accession>
    <name>A0A8J4V5M9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000312</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000006">
        <property type="protein sequence ID" value="KAF2078328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5M9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="174" mass="19846" checksum="2C4BC17A03115B40" modified="2022-05-25" version="1">MEGVQPNLPSTIDGVATNLYIKQAIPNNSDNNNNNNNNDNDNDNNILPGGEVLDQNQLTLNEIRLGFVILSNNRQIEILFPAFETDQIHVVKIRLCAYLRLNAGFIKLTAHHCLDEISFDLDENYFVSQYPYNQLKILVQEIRPDVLSTTNEGIFPIPTYDHTDLEQLILNHQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5W9</accession>
    <name>A0A8J4V5W9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Negative elongation factor B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000108">
        <property type="protein sequence ID" value="KAF2075242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5W9"/>
    <dbReference type="OrthoDB" id="18449at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032021">
        <property type="term" value="C:NELF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034244">
        <property type="term" value="P:negative regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010405">
        <property type="entry name" value="COBRA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13503:SF3">
        <property type="entry name" value="NEGATIVE ELONGATION FACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13503">
        <property type="entry name" value="NEGATIVE ELONGATION FACTOR COMPLEX MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06209">
        <property type="entry name" value="COBRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="651"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="651"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="667"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="678"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="700"/>
            <end position="733"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="742" mass="84217" checksum="49394519FD15BF9D" modified="2022-05-25" version="1">MEEFLIGHLGAENILTNLSQTTYPLQYINQFQNENSIHKKNLPNVSPVLNLLDLHQIHRGDLYYSLLTSLKQNLLQRIEKLSFKELESLLEQTFPYIGFEDLQVIPMSVMKNMTPRVPPAFLLKLSESSELYEACPIEVKRQIWLINEDLFREKIDPLITTYIQDSQLTVELNEMIVVEQPLFAVQLLPAKKRETNAVLQELVAIVGKLPSLYQMLVGHIKKLFSDTCNYALCNLRVEILMTFHDQGLSDVYNRDITHSLAWCVDACIRDYNIDSRRMKDIQSAFHSTNLSNNPLALGDVAMVFANPITMNCVTKSILVQLKEVVKRKQIPKDDESIKYLSSLLTLALEAHSIIKHQSHTTKLPAVKKEILQTFYPILASQLLDDHAREITKLSSSSSTSPALESTQVEEVDSNLPSLYIKYDLCKIVTLSYVVKRVIDKDYPSVDKYLKFLNQLVVNNTKSNVEKNNSDSGSGDNNPGNELDESYDIYPFFGNDFIQTITTNAINSKDLKLNTIIFDSFLLKYRYTKFVQKQLIRYLLENHTKINQKEYISFIQKLYHAVIYTKLSCVGMFTNSNNSTANELDSEDDSTGEDNVNYHFRNSASMTLQQEQRLNDDILIMSKQLLDKSGNRLNEKIVPLLFDYLKSKTTFTPPIHTSNTPTSNQATSISSPRSSTQSGNSPAPVTEQQQDTVMTDNISITESTEQTTTSTTTTSNTEDSTSESMTTTTTTTTSNVIETAMEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6H7</accession>
    <name>A0A8J4V6H7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_002828</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000083">
        <property type="protein sequence ID" value="KAF2075842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="427769at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057709">
        <property type="entry name" value="DUF7949"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25820">
        <property type="entry name" value="DUF7949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035149299" description="EGF-like domain-containing protein" evidence="3">
        <location>
            <begin position="25"/>
            <end position="1365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1321"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="7">
        <location>
            <begin position="406"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="6">
        <location>
            <begin position="631"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="5">
        <location>
            <begin position="776"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7949" evidence="8">
        <location>
            <begin position="1028"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="4">
        <location>
            <begin position="1064"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="999"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1002"/>
            <end position="1019"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2075842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1365" mass="152183" checksum="2752349F9DF80DE0" modified="2022-05-25" version="1">MMRKSNYFVLLLVLNTILLGLVNSFTIQDLTDPKTNDKFADASGRCYNTYYVLLEDPDGISDIIFTETKTLLDNNSTHSLYKLSPVLSTGATSFSFQVENSLGAQTTFNIAYSCPTNPTDLEIEFLSDRVINTGFVISNLIAVFKVNNFPTSKPLPNTLVFTPTSGNIYYSSIGPYYFLVLSYSQSDDISAITSDYTFSISAGGPTYQQSLKSFATDVKALSFTHNISPMSQAFEIKSVFFFSELTGTFDKNEPANIMENSGGTWVQPMYVSGNYTKPILLSRYATLPNTNFESKIVNSDGVSSLGDYTITKINDPNPFAITNPLFEPTSDPIVYSISYTSNYQYSSPVTSDGVTFNLYHPFGYTNGDSNSYLAKFDVFVSRNIDHQISFTTTNTAFYVTPAPLDTVDSTPPKIVSIDYYLLPNTSTIVLTVHITDDISGFFAMIDAYSVVMGDYTNLVKGNLNDGIFEMIQEAPSELSQYYNAIKICDWALNCQSVDVIEVFNVNLAKLDLEFLNFNEIISIKFEKNDIDTTNQSVENKMMLYTTFGDRPHITLISFDKPGPFIPGQYRKFYGHWDDILNCYVYPFTIPKNRFTGTYIYSLNLDQSPTFSSHLSTIFGPDAEIRVTSENADMMGPEVTSVTKNPSTLTVNDGQDSSFEMEFRIKDSVNGLKMAHFSIMSELDLHRYNFTFYPSDAVDGDKYDGLYKFSVPVNGKCRSQKFHVQRVYLEDEAGVQSIYQRFETLDDYNPFIKIIDSLNYEIYTQVNCLAAQESDPPALIEFSFTPTTVDPHYSGTLLNDNDARVVVFNFKTSDDSGILLSALPVVYLQSQNLAPNTILRVDANLISSTDKTATYNCSIEIPYGFGFPHGIRVSLYGIIDNQSNFRGYSLSEMPFPNIISVPTTRNPAISLLSSTGEISTGVLTVFGRNFEQNDKLLIQDTRLNKLYVIEPTLLGDLLSFKKFENIPKINREYVLITIQRSTGVDTFEYSNSLKLYVGGTDPSSSGSSSESSNGNSNSEETPTNPPQKCISECSGNGVCTPNGCICKSPWVGRDCSSQVIIIEPTINSTSPSTNITLPPTSEKIYYAIISIVGMSELDPSGEVVNDYRFNRWIFTKNTNSKYQQFTNETWKYEASITSPLDQSITNVTVFIDYFNSPTAVNITFAGQVLTMNPYTLKYSVNVTSYSFAKSQNSLQLVLSASLENQEKNECSSLEKYDAVESNSEFIKLQVNDHSLYGRFIKRGIVDGRTLSISNTIVENKIYQSSSTQSKSQAYIGINLPYFRNLVQIDPDFSVLIDNKPANPSSMNSICQEKKSKLTGGQIAGIAVGSFAFASVIAVCIGYYIYKKKQSKKLMASIESRLKEVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7M7</accession>
    <name>A0A8J4V7M7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004599</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000165">
        <property type="protein sequence ID" value="KAF2074114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7M7"/>
    <dbReference type="OrthoDB" id="5971719at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098858">
        <property type="term" value="C:actin-based cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031209">
        <property type="term" value="C:SCAR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048870">
        <property type="term" value="P:cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028457">
        <property type="entry name" value="ABI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10460:SF0">
        <property type="entry name" value="ABELSON INTERACTING PROTEIN, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10460">
        <property type="entry name" value="ABL INTERACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="153"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="153"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="216"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="236"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="272"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="316" mass="33394" checksum="BF6CD2302E41C24C" modified="2022-05-25" version="1">MSNEGLDINVYSQTTIPAALAELMDNHSKMEQISNYCKSAYVSGGDTAQIYEQTQGYAKNALLNVAYHIQTIGTHITTLLQLQSNEIEKISIEIATLTQRVNMIHDSTGTQAFNTPDSTKSYRSTLKEKKIEVDSAKAPTKFVRKQVNYSMGDSAQLNHSNSSANLNGSTGGLAPPVNSNSSTPTYQSSPSYSPQPTGVPKGPPAPISAPPQRTSAPPPPSLSVPPIPSQRTSVHAPPPPPPSGGFDLPPPPPMMGDFPPPPPSFGGGGNGLPPPPSIGDLPPPPPSSFDFPPPPARPVSQYGYDFHDLPPPPPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8Q4</accession>
    <name>A0A8J4V8Q4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_003376</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000105">
        <property type="protein sequence ID" value="KAF2075299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8Q4"/>
    <dbReference type="OrthoDB" id="260519at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016717">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042759">
        <property type="term" value="P:long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03506">
        <property type="entry name" value="Delta6-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000007">
        <property type="entry name" value="Sulfite oxidase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19353:SF19">
        <property type="entry name" value="DELTA(5) FATTY ACID DESATURASE C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19353">
        <property type="entry name" value="FATTY ACID DESATURASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015921">
        <property type="entry name" value="FA_sphinglp_des"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="143"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="12">
        <location>
            <begin position="4"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2075299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="455" mass="52541" checksum="EF51CD7D3E21972A" modified="2022-05-25" version="1">MVGLKQYTWTELSKHASEQDCWVCIDGKIYDITKWLPDHPGGKEILLLSAGRDVTNLFESYHPMTDKPASLLKKYEIGVLSSYEFPTYVQKSKFYQKLKERVRKHFNDTAQDPQASVGVFTRLAFVYLFLFVTYYFAHYFTKNFFLGCLLALVYGLGESLFSIHVMHDACHAAITHSPAVWKWLGASFDFCTGASFYAWNHQHVIGHHLYTNIRNADPDLGEGEIDFRIVTPYQPRSWYHKYQHIYAPILYGLYAIKYRLQDNETFIKRTNGSIRVTSPSTFDYAAYIAGKCSFVFFRIVLPLYYGVPLYNLVAFIILAELTLGYYLTINFQVSHVAEELTFYGTAQKPNEPTHIDEDWAVLQMKTTTDYGHGNHMSNFFSGGLNHQVVHHLFPSIDQEFYPQLVPIIKEVSKEFNVPYHIQPTFTAAITAHINYLYKMGNDPDYVPIKLNPKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V9G9</accession>
    <name>A0A8J4V9G9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002608</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000073">
        <property type="protein sequence ID" value="KAF2076094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V9G9"/>
    <dbReference type="OrthoDB" id="24030at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35885:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35885">
        <property type="entry name" value="CARBOHYDRATE BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035307594" evidence="1">
        <location>
            <begin position="22"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="314" mass="35385" checksum="51E816047E389D0E" modified="2022-05-25" version="1">MKFIITLLVFLSIASIGYTRADHNIVIWNSGGVSIGTIDFASERQAFVQWHLPGFLYPNPPYQASTYNYVTKLLTMYVYEMSTKIPYLFVVDCDSWTTLAHQTFYNSAYMYGGLASPNTAASPPTVFSANSLLESKKIFVSRIENWINIVTFDTVIGSYRGSVFDSSIGYNSYYLAFTNQTGLYVRAYYNSQLTREEAFGFSNNLYPVNDEPLNLVYDATTKNVWAQVSMTNPKDGYIYYALAFLDWNKGVFRVSDMYGNAKDRFSATLPNPNGSNFAYSFGVWGGYLTLYTFNTSSESLISDQDLSTPIANAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VAZ9</accession>
    <name>A0A8J4VAZ9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000022">
        <property type="protein sequence ID" value="KAF2077684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VAZ9"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="1">
        <location>
            <begin position="23"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="233" mass="26297" checksum="A69154AA3E8B4E46" modified="2022-05-25" version="1">MSQGEELDQDSVIDENEICRYIICFYGIPGSGKSTILNAILQELYFTNSISLNGSVTSHSAYVCCDGVTFMDTPGLSDPLRKEDIIREIQRSLIKNKHYKIVLVCTLYSGRVSGLDINTINTILSAVPSDVNINYGVIYNKLRPGLMRAILEADPLTSIHSIFLDRQPSSVLLLERLDELQGEKDKLCENQAFVKKIKEFINDLQYSKRKFQEKDIEAPVPEKEESPSLCRLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJ60</accession>
    <name>A0A8J4PJ60_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010457</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001126">
        <property type="protein sequence ID" value="KAF2068217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJ60"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="224" mass="25669" checksum="9DD19CB0B63BE55A" modified="2022-05-25" version="1" fragment="single">EQIKNLKKDDTVYYFSDSKCVNTSQISITNNDGVVDRTIDYPKDPNSKLLIVAYNDTMGISSMKFTLNSGTFCSKLSIDNLFSSRRYEMQLVGNQYKLIQYTPNNLAITSYCDTNVFVQVRIHGTIKKHSFFMQINENTQSLDNVPYLGDIKLIRVKGFGTQVACTKFRITQTCPEGQSKCLPKNSPYRPLQGYRKTFDSKLQTINIGEEYTYIPFYPLGQVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJM8</accession>
    <name>A0A8J4PJM8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009819</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000818">
        <property type="protein sequence ID" value="KAF2068860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJM8"/>
    <dbReference type="OrthoDB" id="20085at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="77" mass="9388" checksum="74F792B99078076B" modified="2022-05-25" version="1">MENNKKQDMINSLHINQYDEKIDFETIQKMKVEEYIQYHIDQQINQLNNHLEKKIQEINEKKEKVKADLLKELQSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJN8</accession>
    <name>A0A8J4PJN8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010356</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001059">
        <property type="protein sequence ID" value="KAF2068319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJN8"/>
    <dbReference type="OrthoDB" id="642895at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051256">
        <property type="term" value="P:mitotic spindle midzone assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007145">
        <property type="entry name" value="MAP65_Ase1_PRC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19321:SF16">
        <property type="entry name" value="PROTEIN REGULATOR OF CYTOKINESIS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19321">
        <property type="entry name" value="PROTEIN REGULATOR OF CYTOKINESIS 1 PRC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03999">
        <property type="entry name" value="MAP65_ASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="182"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="353"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="123"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="203"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="256"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="433" mass="49100" checksum="36212176A4B5F160" modified="2022-05-25" version="1">MDHLKELFISNNFDIDRPCEELLELIDQEYNRCANIYELSKPLLLLVEKRETIRNEKIEYEQNVLGDKERLLTKKPDRGRFIQEERMRKSIQKLPALEENLKKQLIQWQHTHNSPFLYEGFNYLDLMQQQSDQEKKIKEMKKEREKIKACEEMGIKYNGNVNTGNKVSKTTAAAAVSKDIHNTTANVQQHQPKTPTSLRIKSTHFTPSSPKTPTSKSTISKQTTFNNENNHHHNQQMQSPYADRKPLLKKRVPLSPLQSPNKSSVKSPATAAAKKKLIAQHNNNQQQLQINSIKKANIVSSTPVAPVVSSNNKINNIINNNNISIHSTPIQFSIQSTTPLSNLDKRNNISITASASSSSSSSSSSSSSASSTSQVNSSFCPQPNFNASLTNRQLNLLQHQHYPTTTNDSIMGTHYFNNEELIMNDRFESSIMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PK09</accession>
    <name>A0A8J4PK09_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carbohydrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010114</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000930">
        <property type="protein sequence ID" value="KAF2068559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PK09"/>
    <dbReference type="OrthoDB" id="19902at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040310">
        <property type="entry name" value="DDB_G0292248"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31648">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25544">
        <property type="entry name" value="Ependymin_amoebozoa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035174298" description="Carbohydrate binding domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="227" mass="24352" checksum="B1E3EDAD72C9C02A" modified="2022-05-25" version="1">MKFLTIALLVLLSVSASLASNCFETTGYSTNFTATGFNSILPSTNGAALSFFEAGTINVDFTKQQMAVTFDIILTGSMVLGQLYAFGANQTMYVVNIDANGATCTKLPLNIPIPTSFPNITADLGPINIGKFQTEVLEIVENGSQQKVLYDLQNCAVVSSYMINDPSQAPGVTTVNFLNFENTPAPISLPSICTNPSMSIKSNNRHISKSSVITGPKSIQQLLRPFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKC2</accession>
    <name>A0A8J4PKC2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001008">
        <property type="protein sequence ID" value="KAF2068403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKC2"/>
    <dbReference type="OrthoDB" id="10650906at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="56"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="299"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="382" mass="43202" checksum="40957E417F9D9227" modified="2022-05-25" version="1">MNGLSWADETENEERENEIKKKLVEDTKPTTPVAEEPVVEKSQESSNTTEDEQPEQQQQQQQHHQQQYNRDNNRNYGNRDGYNQGGYQQNRGGQMNNRYNNNNSNKFGNQQQQGGYQQNRYNNNNQQGGYNNNNNNQQGGYNNNNRTTYGQNKQNSVNYKRNETLFTGYEETTTKEEFENFLQSKGVEAREITFTEKEAIVGLVDSDNYYKALGLNDAEFNTSRLVVVNYQKKQYYGNQQQQQPQQGSQQQPQGGAYAPKVDPFGGAKPVDTNAPKDVKPADNQPAVSKPYRPGSMGGNNANNNNNNNTNNAFKRDDNNKFGGFNKKPYNNKGPFGKKDEDGWKQVSKGGKNVTQTQVAAAKPIATTQNKFGLLDDSSDQDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKR9</accession>
    <name>A0A8J4PKR9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009990</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000876">
        <property type="protein sequence ID" value="KAF2068688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKR9"/>
    <dbReference type="OrthoDB" id="809632at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045010">
        <property type="entry name" value="MDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205:SF7">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="86" mass="9902" checksum="1D0EBD6A19F8CC07" modified="2022-05-25" version="1">MFVPKVLIDVYFENVGGKVFEAVFPLLNTKARVPVCGLISAYNATSINHSEIALAQFQGAIMKKRMRVQGFIIFQDYFIFYDEFKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKV3</accession>
    <name>A0A8J4PKV3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_009929</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the MICU1 family. MICU1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000857">
        <property type="protein sequence ID" value="KAF2068748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKV3"/>
    <dbReference type="OrthoDB" id="186625at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990246">
        <property type="term" value="C:uniplex complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036444">
        <property type="term" value="P:calcium import into the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051560">
        <property type="term" value="P:mitochondrial calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039800">
        <property type="entry name" value="MICU1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12294:SF1">
        <property type="entry name" value="CALCIUM UPTAKE PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12294">
        <property type="entry name" value="EF HAND DOMAIN FAMILY A1,A2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="190"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="379"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2068748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="439" mass="50156" checksum="93164624EDA04F1F" modified="2022-05-25" version="1">MFARSSSSISKVVRTLNSSNNTTQRIGFRYYSNNTNSNNNNNNYQYNNQQSNNNNYNNNDKRFYSKIGLTTIAAIGLLTPTIVSLDNQQTWREKIINNYQNRIREYSTPEKIFQCFASVKKGGQYYMTAEDFIRAILPHQFKSASETGTKSKSLNLDKNKLPLSFQIADVDGDGLISYSEFMFFSTLLSIPENSVAIAFKIMDVNHDNSIDANEFMSIIRILSTQSEFSKTATATTPNTPISSQGFISHLFGADGKKKLSLEQFQNFLSQLRKDVLNLEFKFYDPQNTGYISQRDFGLLLVSYSKLGKLESHINAINSLPNTVDDKNKGISFEQFASFNKLLDKLEDVELSMDLYKGINQPFSKKQFRYVSKIVCDVNPQPEVVDTVYKIFDTDQNGDLAKDEFVSIMERRKYRGFSNQRDTGIVDKFKRIYKILLGQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLK4</accession>
    <name>A0A8J4PLK4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Translation initiation factor IF-2, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_008509</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000531">
        <property type="protein sequence ID" value="KAF2070167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLK4"/>
    <dbReference type="OrthoDB" id="361630at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03702">
        <property type="entry name" value="IF2_mtIF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03692">
        <property type="entry name" value="mtIF2_IVc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000008">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10050:FF:000001">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000019">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="entry name" value="Translation initiation factor IF- 2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053905">
        <property type="entry name" value="EF-G-like_DII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044145">
        <property type="entry name" value="IF2_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000178">
        <property type="entry name" value="TF_IF2_bacterial-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00487">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF5">
        <property type="entry name" value="TR-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22042">
        <property type="entry name" value="EF-G_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="Initiation factor IF2/eIF5b, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="10">
        <location>
            <begin position="438"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="83"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="301"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="716"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="127"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="252"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="329"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2070167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="973" mass="109648" checksum="F35084F0ED8F57EC" modified="2022-05-25" version="1">MLRSSSYKNYILYHQNSKTSVWVCNYATTPSGQPTTNTGKSNGKEFARIDFSKINKAGTTAENDTDTKPNNTLKTFNFNFNFNFNNTPNTNNNNNNNTTTTTQPPPSFGVSPNTTTGTTPMFKFPNLTKSAPQQTDSTSSTTEQPKKPMFPGLNSFGGNFQNTSANNNNNNNSNLNFTIPSKPANQNTIAATSPFLNFFNNNNSTTESKHVNDVGQNIKLSFSNSMFNQTSFNNNNNDSLMPNLSFNFNNNKQQAQQQQVQSTNTYDQDEDMDQEELDGEVVEDNSMIMDSFKSLEEKYTNAMKKGAKQQKQFQKGMNVESARRHSREKSKFDTNEEEEQQHVDKRNKKKVFSMRDVDIPSTATPENLAPLVGVQVIDIIKLMITERLKPKSKNEILDQGIIDLLAEKYVFRPIYMEENELDHEYIEKDKPMNPSWPKRSPVVTIVGHIDHGKTSLLDYLRKTTIVEKEAGRITQHIGAFEVKISSGEKITFMDTPGHAAFSTMRERGVQTTDIVLLIIAGDDGVQEQTLEAIRAIKKANVSQMIVVINKVDKQGVDPELVKQQLLTHGLPVEGYGGDIPCVAISAKTGQGIKELEETIVMCSELMELRAPIKDVQPSAIVIESTFKKSRGTYASLLVRRGVIKPGQWFVCGDSWGKIRDMRDHLGSVVKETRPGCPVEISGFKEKEPNPGDQLYIFDSEKRAKELIEAKSERRVREDTKAEMIKKNEKIKQAEQETPEEKALQEELEQQQNQRKCVKLFVKADVGGSVEALVTALQSIPYDDEIYYEVVKMGYGEITPTDLNESDFLKTPIVYFGTKVSSRILDEARRLQLPILQSDIIYHAVDHVAKFLEDQLDPIDAYDIMGEAKILNVISINKTNSEKVNVAGCKISDGKIKRSSRIQVKRNDQVVFTGMIDEMRHFKEVVKEVQKGQECGIQLEGFDKFENGDTLLAFNMRKERRKLGQTNAPVINYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM68</accession>
    <name>A0A8J4PM68_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000624">
        <property type="protein sequence ID" value="KAF2069639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM68"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="70"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="225" mass="25114" checksum="AFBCF5D97A567DCD" modified="2022-05-25" version="1">MKRNISPSTSDNEDQHNDSSDHQQQPQQQQQYKQLKHDNEDDYDTISNTSTSSKRQQDDSDEYEEEIVEESNASSLYLPSTTNNNNNINRNNVMNINSLSNLLSPSSPPSTSSPALSSSSLSYSNSSTAAISSYTGGFSTKSINEDDEEEESYDNSESESTSVIKPNTTTTSTSISKATLKPTQFLIQRSSDETPRVVDLYSNNDERYNETQTTMFPPLTYESFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMB0</accession>
    <name>A0A8J4PMB0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">U6 snRNA phosphodiesterase 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">3'-5' RNA exonuclease USB1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_009808</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000814">
        <property type="protein sequence ID" value="KAF2068871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMB0"/>
    <dbReference type="OrthoDB" id="49151at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034477">
        <property type="term" value="P:U6 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027521">
        <property type="entry name" value="Usb1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13522">
        <property type="entry name" value="U6 SNRNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13522:SF3">
        <property type="entry name" value="U6 SNRNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09749">
        <property type="entry name" value="HVSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="LigT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="16"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="240"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2068871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="309" mass="36480" checksum="F9D70D21C4751CA1" modified="2022-05-25" version="1">MNLLCQYEDEIEQDYQEIQPLQESKEKKDDKRKRITIEQEKVDKQDDDIPDPFFKKAKATFTQPAPSQDRNKDEKMRQFQHVEGNFPTFIFIKIPTDRQDIIDLVQEVNTLSDSVLNNDNEDHQVNLNIKQLKDYHISLSRTFPIREHQIESFSNEIKKSLSNQKKFFIQLESCNLFYNDNQSRVFLSMLPTQSSKSTLLDIIDRIDKCCLLFKFPLFYKDPEPHFSICWKSLINNNNNDDDDDDDGDNEDTETTTSTTATTDSSSKEILLKYKNNTTIKKKMDTIINPIIVNRIYWLVGKREYYIELT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMG3</accession>
    <name>A0A8J4PMG3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CYRIA/CYRIB Rac1 binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009541</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000736">
        <property type="protein sequence ID" value="KAF2069139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10265867at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009828">
        <property type="entry name" value="CYRIA/CYRIB_Rac1-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008081">
        <property type="entry name" value="Cytoplasmic_FMR1-int"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12195">
        <property type="entry name" value="CYTOPLASMIC FMR1-INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07159">
        <property type="entry name" value="CYRIA-B_Rac1-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05994">
        <property type="entry name" value="FragX_IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008153">
        <property type="entry name" value="FMR1_interacting"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01698">
        <property type="entry name" value="CYTOFMRPINTP"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CYRIA/CYRIB Rac1 binding" evidence="2">
        <location>
            <begin position="62"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1257"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1280"/>
            <end position="1328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1328" mass="152193" checksum="797F767CA1F0E990" modified="2022-05-25" version="1">MSVDQSKSKLADVFERVDILETFPFRDDQSEIETPSSTIGYQNYDTSINYADRGAFETQWSDETQSMEKMEEILKVGEGFINMVYTYRSCSKALPQVKTSEQTNKQQIYEGTFEVLEPEIKKLKDFMYFQKDTIKFFTDRIKNLAGSYDKKKETIIASETFICYLVKILDLLAILDALKNMKACLNNDFSFFKRASGFLRKQMSGVEDQTQENHTLYLFLANQNSITTTLKQELHNIEKFDDVLPIIVNQCADFLEQEKYVLPSEKHCLLRVMPFLLFLIDENDPKKNIFKNKSLNLSRYSKIFRKNPVVPLYGDMQITLESLVKHSPHFDEKAWGTSTLDSKVAFDYEIIHVLDATRIHYNECMSRFSNMVNEIKSAKQRKDTPAGVYPISDTEIKQIVLHCLRILSDWTSRVLQQSAWKYSKANNDPTINATFDYERVVKYNYTADERTALIQFIAMIKSLATLIIKNEALLQPIIRRCIHQEIQEFIQISLRETIKSFNKNNPKKKDNIKSEMAQLKAIAADWAAGVEPVEAVPSKKSSKEVEEKLVLPPRGVPPSSTQLELILNLVSSLMDKRKDFSSTQNQEFEDFKNKAFFYRYLLNLSTSVNSITDLADLWYREFYLELNNRVQFPIETSLPWILTDHILESDDPSLIEHIFYPLGLYNDTAHRALLHLNQRFLYDEIEAELNLCFDQLLFKLSGKIYTHFKIQASSILLDKSYKQQLELAHFSGKLHTPKSRFDVLLRQKHIQLLGRSLDLVGLLAQRQNTLIRQNLDYAISRFEASDLTSIIELETAINNIKLTYKILSEYFELDSFDSIFNEVNESTSLVSYHGRIVLHIIYEIVSDFARNYTFNSITQRFVKAPYLFTDEVQREGMPKTNPNFLFGNKPLNAAYANSAELHKYFIGMPHIQSILRMVGKKNLPLIVSETLRNIEIKLTSVLSPYVAELLAGMPASTKLPIYDYGTEGGYGYFQLKLKDIYIYPDLRPEVLQCFRELGNSLVLMNLLDQAITQTDSYNFIKSAPFLGITPETWFADVPEEDPTINSPLYSQLLKLAQLLETKPEVTKAPVALNDIISNAWRADKFYRPSAENHSLFKSVLQRFSFILNMVRNEWSGQAPDNGVINVDSSTEFYRLWSALQFVICWPLSNENDKSFHELFGDGLMWAGCSLIHFLGQQYRFELFDFCYHILNVEEAAAVRSDKPALKNFFRAAQIIKDLNQQIFAILNTYSPPPPCSNMLLSPPATEQAEQFIVTTTVEETSSNNVPPPPPSNSAGAIPNSTPPPPPPPNSSNSFPPPPPPFEGNLPPPMIDDMPPPPPFGVPPPPPPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNE2</accession>
    <name>A0A8J4PNE2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Short-chain dehydrogenase/reductase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_008245</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000492">
        <property type="protein sequence ID" value="KAF2070434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNE2"/>
    <dbReference type="OrthoDB" id="6251714at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05346">
        <property type="entry name" value="SDR_c5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000047">
        <property type="entry name" value="NADP-dependent L-serine/L-allo-threonine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42901">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42901:SF1">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2070434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="263" mass="28873" checksum="2CCB5CE48B18EC41" modified="2022-05-25" version="1">MFRQLSNKIVLITGASAGIGEACAKIFYQNGNHVILCGRRLDRLNKVKEDLEKQQQKEANKYKVLAVEVDVSSLDSIKKMVSNLPEDMKNVDVLVNNAGLALGTDLIVDIDSGKDLDAIMDTNVKGVFRVTREFLPGMIKRNSGHIFNVSSIAGSMFYPNGGMYCASKAAVNAFTDILRKEVVATKIRVTNVCPGLVETEFSLVRFSGDAEKAKKPYLGIDPLTPEDIADNIYYAASRPEHVQITTIETMANNQASTTVIHRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNE4</accession>
    <name>A0A8J4PNE4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000504">
        <property type="protein sequence ID" value="KAF2070343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNE4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32556:SF18">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32556">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="398" mass="47133" checksum="F7FCB1F7C421A98A" modified="2022-05-25" version="1" fragment="single">QQQQQQQQQQQQQQQQQQQQQQQQKQQRHKKQKIRDFKILTKESVQHINVELAQIGDLSFQKINQYTSLRSITVVHSAHDSVILSYHKLEVFNKLKRIDKINVNFFFYISSYYEPDILPEIQAITFKFKTLHFLVFHNNHNDKSFNFIYGLINLLRPNSVELHSLDDGHLTFFHSLSKLSFESVVINDDYIPLYSLYRFLGSTRLTLFKFNLQFDFLSELYDNNNNNEFNYDFNLMDTTDGRKINEDNQKANYIDGRYSHIPEYSGPLWKECLQLLKTNTTLTELSIGHCQCDYLEILNKQLIDDLLDSLSKNKSIKTLYLDFGYRRNRHSIINADFISSLLQNNTTIKSLYIRDERYSRWDGLLDDIVNSKPPSSKCFIGYAPDMPISFQFSSKAYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNJ0</accession>
    <name>A0A8J4PNJ0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UbiA prenyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_008013</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000461">
        <property type="protein sequence ID" value="KAF2070668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNJ0"/>
    <dbReference type="OrthoDB" id="203513at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042371">
        <property type="term" value="P:vitamin K biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13962">
        <property type="entry name" value="PT_UbiA_UBIAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1780">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026046">
        <property type="entry name" value="UBIAD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929">
        <property type="entry name" value="1,4-DIHYDROXY-2-NAPHTHOATE OCTAPRENYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929:SF0">
        <property type="entry name" value="UBIA PRENYLTRANSFERASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2070668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="255" mass="29079" checksum="6A8800D3147BB40A" modified="2022-05-25" version="1">MYLVPLLVSTYVICNVLNALCDYESKIDEKDTSSDRTMFDYGLTKVDVWNIVYFTMAAMVGATVMAFSHFTPMDQLWMLMYTGAIILLAVYYNLPPLRLKTKFFGAEATIFLIFAIQVNTAYSLVVGKPSYSICFTDYPGCFLKIWAIMMNLYVDSEEDKKDSSKSIPIVLGDKIARNLIIFTSIMVSLLTIKYAIQHNNIYVCLSLLSIPLKFTAMRLAFRKQKNVKPLYFKSMLIYNVFFSLGIIINKTLVGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNQ9</accession>
    <name>A0A8J4PNQ9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 14">6-phosphogluconate dehydrogenase, decarboxylating</fullName>
            <ecNumber evidence="10 14">1.1.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_007420</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10 14">
            <text>6-phospho-D-gluconate + NADP(+) = D-ribulose 5-phosphate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:10116"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="EC" id="1.1.1.44"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10 14">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10 14">Belongs to the 6-phosphogluconate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.44" evidence="10 14"/>
    <dbReference type="EMBL" id="AJWJ01000395">
        <property type="protein sequence ID" value="KAF2071262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNQ9"/>
    <dbReference type="OrthoDB" id="434986at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00410"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0004616">
        <property type="term" value="F:phosphogluconate dehydrogenase (decarboxylating) activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="P:D-gluconate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:pentose-phosphate shunt"/>
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        <property type="entry name" value="6-Phosphogluconate Dehydrogenase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
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    <dbReference type="InterPro" id="IPR006183">
        <property type="entry name" value="Pgluconate_DH"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="PRK09287.1"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR11811">
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    </dbReference>
    <dbReference type="Pfam" id="PF00393">
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    </dbReference>
    <dbReference type="Pfam" id="PF03446">
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        <property type="match status" value="1"/>
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    <dbReference type="PIRSF" id="PIRSF000109">
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    </dbReference>
    <dbReference type="PRINTS" id="PR00076">
        <property type="entry name" value="6PGDHDRGNASE"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5 10 14" id="KW-0521">NADP</keyword>
    <keyword evidence="6 10 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 10 14" id="KW-0570">Pentose shunt</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase C-terminal" evidence="15">
        <location>
            <begin position="181"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="33"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="75"/>
            <end position="77"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <begin position="129"/>
            <end position="131"/>
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        <ligand>
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        </ligand>
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        <location>
            <begin position="188"/>
            <end position="189"/>
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        <ligand>
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        <location>
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            <dbReference type="ARBA" id="ARBA00002526"/>
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        <source>
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        <source>
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        </source>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000485"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAF2071262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="488" mass="53457" checksum="330AB87C38AA9624" modified="2022-05-25" version="1">MTKADIGLIGLAVMGENLVLNMESHGFTVAVYNRTTAKVDEFVNGRGKDKKFVGCHSIEQLVQSLATPRKVMLMVKAGEVVDHFIDLLVPHLEKGDIIIDGGNSLYTDSDRRVKSLAAKGLLFVGTGVSGGEEGALKGPSIMPGGNPAAWEAVKPIFQGISAKVQPGDQPCCDWVGDGGAGHYVKMVHNGIEYGDMQLISEAYFIMKHYLGLNNQELQDVFTEWNKGELNSYLIEITSDIFGKKEDGEYVVDTILDSAGQKGTGKWTAMNALDLGVPTTLIGESVFARCLSSLKDERVKAAKILPGPNASESYKHFQGDKKQFIEYVKQALFASKLVSYAQGFTMMKAAAKEYGWKLNYGAIALLWRGGCIIRSTFLGKIKEAFDHDANLDNLLTDCWFTEKLQEAQTGWRQVASVSLLTGIPTPAFTSALSYYDGYRCPTLSANLLQAQRDYFGAHTYEKLNGKKGEFFHTNWTGRGGNTHSTTYNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPI6</accession>
    <name>A0A8J4PPI6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_006918</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000340">
        <property type="protein sequence ID" value="KAF2071772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="15055at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="42"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2071772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="23274" checksum="E097560CD5BED2B0" modified="2022-05-25" version="1">MEEDYQEIIWNEYIKRISNGDYEGVRELMNQFAIDINTVDSKGRSLLHVAVSRNHIHIVRLLLENGIDTTLADVNKNTALHLATINGYSSIVSMILEYNSLTNVNVVIADKNNNSPFELAISRLKRSIESSKTNSISPSDRLNQISEMVYDLAQYIHQHLGPNHPYTIKIYSTIDRLKQLEQIKDTVDNKQVDEIQDLLSGLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPM4</accession>
    <name>A0A8J4PPM4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006784</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000328">
        <property type="protein sequence ID" value="KAF2071905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPM4"/>
    <dbReference type="OrthoDB" id="18757at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053369">
        <property type="entry name" value="SrfA-induced_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32256">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32256:SF17">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035319244" evidence="1">
        <location>
            <begin position="19"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="316" mass="33932" checksum="F63AD416AE01EEB8" modified="2022-05-25" version="1">MKVLIVLFLLVIINYSSSVFGATCTQNSDCSTGVCRNRLLLFSGLGLNTNNNRILVMGNSPVAPAPIIASVPTSGGSIKGEYQILSNTATRTAHGIFKYLPSSQLLFTINSQTSAPYFVGPYYQNGTLVDVWETNDMSIEIDFDETNQETLLCTTGISLYNSIPITTGSSSSPVYKNVKCQGLGRVGTTLYGSKSSTTGSDFFSGDVGCNNCEESQLNPLFSETFQVSDLAVTSTNIYYSSVWSDGVNEVKGIFEVPINGDLTQKRVLSSDSVEKILVSNGVIYYISNGKLKSVTISSATVSTLYENTGTYQGECA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQG7</accession>
    <name>A0A8J4PQG7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MRPL25 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006503</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000303">
        <property type="protein sequence ID" value="KAF2072183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQG7"/>
    <dbReference type="OrthoDB" id="18529at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2072183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="167" mass="18912" checksum="5E32D2762ADBDA62" modified="2022-05-25" version="1">MANVVKHMINVEALVKFSKPQRSSSGWHQPKISGRQLAVLEKHCTRQLGLEWPLAKEKTPIPERPSKLTIWERNNVLRQRKIQESIQNMPKLLADKIKASREKKKKEDENTITALIPEYVEGGPYPCHLPSKVMALKRKAAMEKEKKITTLLASASAATKKGGKKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR38</accession>
    <name>A0A8J4PR38_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006968</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000347">
        <property type="protein sequence ID" value="KAF2071707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR38"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="80" mass="9163" checksum="AF9FE8558257294F" modified="2022-05-25" version="1" fragment="single">LYNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNSISNSKKPEFDSFDVSSDDNDIETKKSEQMKHGLELKSISEDDNKMTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR50</accession>
    <name>A0A8J4PR50_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000271">
        <property type="protein sequence ID" value="KAF2072535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR50"/>
    <dbReference type="OrthoDB" id="24162at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="400" mass="46248" checksum="A7D707BA47BBDEF5" modified="2022-05-25" version="1">MIKKVNFKYLSLLSNHDKLNHNIRVYLLIQSHDEFKQYIDNQCAASIVNYLKLEEDLNDDLEFYDYSNYQDMIPYSLIPDSVHTLVISPSKHSQNSGPRSRFQQSITKLYFYCHIERSEFVEEMLSHLPSKLETLSLQGYHFIGTPLSKRFVLPATLTEFTYQSNWDNLQRFVAPPNKELGATLLLNDDRPIEALEWMKLQTWVSGVDVISNFHCFINDVLPTRLKHLRLPYHIYQLSRDKLPSSLKQLYMVNYNQGLLPHVLPNGLEGLCLWCFNQPLQKDSLPQSLKNLHLSNYRKSFSSVGPLNNLQRLVVKILNKSVSQLIANVKAMTIIFEKIDNDVTFQNTSLRELCLLNNGSTVIQLNSNILPPTLEKLKTAKIDILFPNSDSSEIQETDKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR87</accession>
    <name>A0A8J4PR87_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MRH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007243</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000375">
        <property type="protein sequence ID" value="KAF2071435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR87"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23524:SF1">
        <property type="entry name" value="MRH DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23524">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G04850)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035188799" description="MRH domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="972"/>
            <end position="997"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1014" mass="111340" checksum="AB23FA4C76F2D952" modified="2022-05-25" version="1">MNNKIYSSLFLVCILFGACFGCGLEAFTYSTDRECNEKYFSNAFVFIGAYQQQYQYSFSPVLNYGRFDNKGYYYYLSSNVTYTLTWTLISGADKSCDGQETFTFDNPSVTLEQPKCSHSYGKINYNGPLDGILLDDGTTPASFPISSQASSDPVKLYFDQDTFLCPYNVKFYELSNKYPVYKIQNPVCGDSQGSVSLDTSKYRVAELYDQQSNKLASTGNGVWSGLKNQTYYFMLESDECGSQKVPFTLEQNIPSFNIEYKQNTCPTEKKAAINVGQWTAFSAKIDGQLVDPTNIDLKSSQLYTLSLSNSDCTFESSFNTPDVELDVKHIVTYPSNCLDPIKVDLVYNEAIITDLLVKNESDIIALTGKSFQYRGSSFSLESACYGTKKVFVQQSNGVVPEYSLTKKPQFAGDKLNVAILNAQSFESLKITNGANQVLPSAEGVFSDLTTYNYVLEYTTSACSGVQYLPLNFAMQVQDRHLVEQVDIIEYAGCGIKGKGSYSIKYTPLNIVSQPTIFEFDSSLVPISNTIQWDGSNQVVKSFKFTPPLLKRNDINVSITVVKGPTCAYSEDASIAIKSVVPIGKITIDGFIHLPTKASNSTHYIFINVPSGELVVTISLSSGCKFTKTVVVNPVNKFEFKYTATGVSDCSVSDGSIQVSNPSLYSELMINGKNILANGELTGLASDYQLVSFILTDNTCFGVAQVYIPTQVLATPVIKDINVPYCQQSNTGVVEFSLKSNTNQPMTLDSVVDYDSTTVYTNGRIEKLLPGSYKYLFKSGSCSWSAPHQVSSQDFQFSSNLLSSFVAGTCSQRTFTKISNDGIIEDIQFYDASGNKESESLGNGYFTYSDVDQASLGVNIYYGQDTCHKSIKITDYVAKSTLQRAFPEFAVSSNCSESYVYPQYTISASNPNNIPIRLGFSDPDSTGKWVVPLLFDSTITDPVTGCSSVSKLFVIDACLPDNNGKEKDKGINLGLVIGLTVGLTVGCALIAVAGYIVYKKKKRSSIGGTKLKNLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRB3</accession>
    <name>A0A8J4PRB3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Folylpolyglutamate synthase</fullName>
            <ecNumber evidence="17">6.3.2.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Folylpoly-gamma-glutamate synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Tetrahydrofolylpolyglutamate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">CYY_005887</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>(6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n) + L-glutamate + ATP = (6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n+1) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10580"/>
            <dbReference type="Rhea" id="RHEA-COMP:14738"/>
            <dbReference type="Rhea" id="RHEA-COMP:14740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:141005"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>a monovalent cation</name>
            <dbReference type="ChEBI" id="CHEBI:60242"/>
        </cofactor>
        <text evidence="17">A monovalent cation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 17">Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 17">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.17" evidence="17"/>
    <dbReference type="EMBL" id="AJWJ01000246">
        <property type="protein sequence ID" value="KAF2072813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRB3"/>
    <dbReference type="OrthoDB" id="5212574at2759"/>
    <dbReference type="UniPathway" id="UPA00850"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.10:FF:000008">
        <property type="entry name" value="Folylpolyglutamate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="entry name" value="Mur ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023600">
        <property type="entry name" value="Folylpolyglutamate_synth_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136:SF5">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038895">
        <property type="entry name" value="FPGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="MurD-like peptide ligases, peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01011">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="13 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 17" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="19">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="433"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="450"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR038895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR038895-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR038895-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAF2072813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="608" mass="68426" checksum="D120158EE0F80DEB" modified="2022-05-25" version="1">MNSVKRFLFDTTHQHLLFSSSSSSSLSRSFIQCSRNNRLQCSSNLFINRSIDKNIIKRSISGNTTAANSNSSNSNFTLNHSSDALNSNSNKNIVDMNIHIPVDNTYEEAINTLLSLQSNQGVILSWINQRKNDKEGATKILLEDMLGYCRVLNINLNDKQSVIHVAGTKGKGSTCALVESLIRNQGFSTGLFTSPHLLSPRERIRINGDMISKELFTKYFWIVWDTLRQNYKDQEMPNFFKFLTLLALKTFQEENIECTILEVGIGGRMDSTNIFPRPVATGISALGYDHMNVLGDTLQEIAGEKAGIMKTDIPVFTVSSQPSEAMEVLVKQSKTMSSPLCVAPSLSEYGFNDKISLDGQHQRENCALALALANCWLSKQTKIPLENIYSNVEENKHQYKQYQYNINNYSSAFFKPLSPSIQKAITTCEWAGRAQHYVMDQYPNIDFYLDGAHTPESSLVCLEWWKTVVNSSATKDSDKDTHYIFVYNSTGGRNPKSFLQPFITSIKDKEIPMFDLSIIPSITIDKTLDKNSMLVEKAQVWEDFVVKCFNELSEDSCKVVASKNVDNCLESIIEYSDNGKKKVKVLVTGSLYLVGNALKFLMKDKAFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRG3</accession>
    <name>A0A8J4PRG3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007072</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000358">
        <property type="protein sequence ID" value="KAF2071600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRG3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="22504" checksum="B053D6BC7930D6B4" modified="2022-05-25" version="1" fragment="single">MMEWDHFQILVDKIKHEPHIIYYPSAKQTIHLIATLLNSDQHLELLELLYQQLFLTFSANEINVENVLNVSVACNNTTFYKFIQSKGHCINDKEEALIMAASKSNLDLLQLIIKNPPKIKNSVETWQNVLIKALENNNHEMVEFIMKYLTLGMIPHNPFYKHCLMSNLKCVKAIERAFPTYFHVSQTDFLKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRZ2</accession>
    <name>A0A8J4PRZ2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Importin subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_006772</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the importin alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000327">
        <property type="protein sequence ID" value="KAF2071915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRZ2"/>
    <dbReference type="OrthoDB" id="29145at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2071915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="467" mass="52886" checksum="800B3262E016656D" modified="2022-05-25" version="1">MNSTIEKINSNELEVIQTGLNDLHRLVSVQIDPPIKQVLDLNIIPKIISFLKHDNDQIKYNAILVLNSLSRAKNSKVISTFLKSDILGPIEELLSNNYNNQKDLKEQLVEIITNIVKKDRDSRYQVLHFNNIVSIIAANIKNGQVKSHSVSTKCIAALSCTKPPPDHFYTAQFLSYLAKYLELYNNNTADFAAENNQKQQDECLADTLKSLSFISDNEHNSDPIVDIVNAHRCVLNLVKLLHHSSDNIVKVALRILGNLITGDEECTQKVMDSPHFLESLKNLLSHPTKATRKEVIWILSNTLADSPHQIQMVIDAGFVPLIIDMVYTEGGSIIKECNWVLLNIVTQGNQQQIQYSLDQGFLKALCAIITKLMHFVGNFENHEEVEEEEDEEGLPSNMIFPMAFDAFELLIKQSSNNPQEPSVINNIYLDETLRLEYQTLLTNFSNSDNYGLETRTRATQLLSSYFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS87</accession>
    <name>A0A8J4PS87_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_006401</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000293">
        <property type="protein sequence ID" value="KAF2072294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS87"/>
    <dbReference type="OrthoDB" id="18650at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17330">
        <property type="entry name" value="MFS_SLC46_TetA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF111">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="314"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="353"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="386"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="414"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="476"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="88"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2072294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="525" mass="57885" checksum="418BAA302EB42F59" modified="2022-05-25" version="1">MKSQLVQGSDCIRENSNSNGSMIILNSTPTLNEIIDHVASNSSLITVLHRSDDSMFLNNQNQNKIVEKEDDFLVEEKIPIISKRQWARIIMIYMAVFSDALAITLITPFLSEILVSRWKVQESHVGYFSGVLIGIYSLARFISGFTLGHLSDKYGRKPFLVLSLLATGCGTVLFPLLPNVYLALFFRFLEGILSNTMALCQATLADMVDKKNRPVVFAYMGSVFALSRCLSSAIGGFSVKIAGNSTNPYLLPCIFGGSFVLLSGLLILVFHPETHPKLAKKSITEEIVEPTLNVEQKEYSFFEGLKLITKNRTVLHLMVMGSLNSFANGGLLLGITLFASLKRPEKGLGFNSFKIGILFTVFGLVGFAFQLLFFKRLSKSLGLKKQYLMGMILLSTSMLLYPVIYTGYLIQGEAIVWVFSIFVVIIMGIGWMQAMSILQGMVANASDPKIQGLTQGSYQSLNSLLRALGPMVSGSIFSLSINWSAPFILFIVLAQFYLACGKISTSLTESVDVLKKKDESQSGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSN1</accession>
    <name>A0A8J4PSN1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CDAN1-interacting nuclease 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_006453</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000298">
        <property type="protein sequence ID" value="KAF2072230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSN1"/>
    <dbReference type="OrthoDB" id="1272at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029404">
        <property type="entry name" value="CDIN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31661:SF1">
        <property type="entry name" value="CDAN1-INTERACTING NUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31661">
        <property type="entry name" value="SIMILAR TO CDNA SEQUENCE BC052040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14811">
        <property type="entry name" value="TPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2072230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="316" mass="36237" checksum="21011545C8E645CF" modified="2022-05-25" version="1">MIKRSDFNIIFKNLNDKDDINILKTQFPNISYSTFVAIFSQKRSKNYRNLCYRKIHNLKQHYNSFINSTYSFTEISIQIGISSSIFAKQMLTYIIECEPQCLFDPNLKPNQYSSTELGKLVLGQPTSTTTTPSTTTATANTNNLETQAKPITDTQTIVKTIFKKPNIIGNPLLRSNILQSIYNDDNCSPLINHIRSSTGSEYEYLLQEKLFKLNIPYLSESELRKNGYPKTPDIKLEVPIAYKGQIINWIESKASFCDDNNFRNTIDQIIGYKNRYGPGLAIFWFGFIEDLNDMQEQGVIISDCFPDLSDIEILCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSU1</accession>
    <name>A0A8J4PSU1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_004796</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the UTP23/FCF1 family. FCF1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000176">
        <property type="protein sequence ID" value="KAF2073908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSU1"/>
    <dbReference type="OrthoDB" id="76105at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09864">
        <property type="entry name" value="PIN_Fcf1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000035">
        <property type="entry name" value="Fcf1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006984">
        <property type="entry name" value="Fcf1/UTP23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037503">
        <property type="entry name" value="Fcf1_PIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12416">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN UTP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04900">
        <property type="entry name" value="Fcf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="PIN" evidence="7">
        <location>
            <begin position="67"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2073908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="197" mass="22880" checksum="68140CFEDC067B6F" modified="2022-05-25" version="1">MARKPSLAPKRIPIQKLNHTNTNYTNRQKQVERSKKEEEHRKKLEAVPEDNTSHMFFSYNEALVPPYHVIIDTNFINFSISHKLDILQALMDCLYAKCIPYISDCVAAEIERLGSKFKVALRISKDPRFQRLSCNHKGTYADDCIVNRVTMHRMFIVATCDADLRRRIRKIPGVPIVYLKGKRYTIERMPDAFGAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT43</accession>
    <name>A0A8J4PT43_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mediator complex subunit 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_004617</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000166">
        <property type="protein sequence ID" value="KAF2074088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT43"/>
    <dbReference type="OrthoDB" id="203279at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009332">
        <property type="entry name" value="Med22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12434">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12434:SF6">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06179">
        <property type="entry name" value="Med22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="11"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2074088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="21996" checksum="C38C90237F5FE172" modified="2022-05-25" version="1">MNQASAKQSAQQQQQQQQQQQQQQQQQQQQFQTRGEVLFRDQQYFQKQIDGKVNQLLENYHNIIKLSKINEKNTSEFYEMETRTTNMLNASEGLLKTIEELKQNLILNDFSTMTEEVRLQNLVYHRENERTNRSLKLISEEISKALEELEHEYYNSNYKLKPAPISSILTPNHSSSSSSITSPCQQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTE7</accession>
    <name>A0A8J4PTE7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_005320</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Mitochondrial ribosome (mitoribosome) assembly factor. Binds at the interface of the head and body domains of the mitochondrial small ribosomal subunit (mt-SSU), occluding the mRNA channel and preventing compaction of the head domain towards the body. Probable inactive methyltransferase: retains the characteristic folding and ability to bind S-adenosyl-L-methionine, but it probably lost its methyltransferase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000208">
        <property type="protein sequence ID" value="KAF2073375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTE7"/>
    <dbReference type="OrthoDB" id="421327at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052571">
        <property type="entry name" value="Mt_RNA_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015324">
        <property type="entry name" value="Ribosomal_Rsm22-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13184">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13184:SF5">
        <property type="entry name" value="METHYLTRANSFERASE-LIKE PROTEIN 17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09243">
        <property type="entry name" value="Rsm22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="79"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="141"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="325"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1027"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="913"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="101"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="141"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="289"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="336"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2073375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1220" mass="140377" checksum="1F27C865FD4B5F67" modified="2022-05-25" version="1">MFLRINTNSLITSRLINATTSSSCNGGALIVSLNQHSSISLSSHLSKQYQLLNNNSGCNDRYYSSLRAGSNKRFMLKESMKRKKRSMDLGNIDRRKETPAKQKSTTATTTTKDAEGKLKTKKSDYNEKMIRMKSSLLEEYLEQEDEEEEMEMARKKGDNAMQQEEEDEDDGLDSQLNFDINEDEGYRQDEDEDDDDMFVKEGETPEFIQENKQAIDEEMRIEYWARQMALRDSLKNDGGNFLLNEHEYKEPIKRYKKLARHLMAKRRQEKIAEAEEQRMEELDRYQMEADQNNGIDSRTSEQGAIVDDAVLDQLLSAEDRKALERQKKKQHKQQVEEYDEDDDEEDQDDDEYEDQDDGLPDLTRLQPYTPVPAPIDIVADFIEAKKQELLKKTDEAADEFERQRKIKRIEFIAYKKALQHRQRLENLKKRGMDQKPDLGEAPLVIDPHIFHRFQSGEITEQEMLELCKQKDDSLDDYFPSTAAAEQDQDLDDDDDSNSSKKQREFLLHWKIGTVELPSRLKRKIAQCIKGYSTDKLREDAATLSDALRERTRSEKLSAKSPVVIKPEDKPVITYGQGQALAYISHRLPGVYGCTHRVFSEIATRAPNFKPKTMLDFGSGPGTVIWSAREIWGESLEKIRAVEPSSFMIDVAKKMLEGDTNDIRWTQFLNTNARGAAGLSGREIPENEQSDLVVASYVLSELPDQATRERVVKDLWAYVKPTGMLVLVEPGTPIGFGLIKESRQMLIDLPPQSLTVHKSVKAQVFGPCPHSNQCPMGHNSWCHFSQRIVRPVFQKLAKGPQSTVPFEDEKYSYIAMTKLVESTIPNQLDKQIGIYGQENLQPTKEWSRLIEAPLKRGGHVIMDVCTPDVDIKRATVARSHGRQMYKEARKSFWSDAFKLDKDKVDWMIARIQKRGGEEAEAEQLKKQLEEAAAKKQKKYSVQNNVLSVVEEIKKEQDKRALLEKKNNINSGSSTTTKKQQQDWMNDMDNADRSILQQLIKQGKFSANEQSNLFGGDDKYSIPEPVIEDGEMSSNSKDNNKFPAPGEYGSKSMDFAFEEDADDIPDDEDADKKSTVNKQELVESAKSRFHRKQKLEQDLKALKRQQMTPQERLEADRAELEEKKKWDGRIHNPKRLEYYLERFDQLEEPISPAAVLEKELEKQSTLQDMAKKYKDTKGLGIASKTMREKISKQKDYKERVKILREKVKLNDLTNNNNDDNTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTQ6</accession>
    <name>A0A8J4PTQ6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Multi antimicrobial extrusion family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_009400</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000699">
        <property type="protein sequence ID" value="KAF2069281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTQ6"/>
    <dbReference type="OrthoDB" id="2126698at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="23"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="109"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="253"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="332"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="365"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="403"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="431"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="518"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2069281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="564" mass="62674" checksum="0452C153A68E3AA3" modified="2022-05-25" version="1">MDEVIKGYFHKGFRTVKSSKEEIVFLLSWTWPVLISNILNQVVYLLVNMIFVGHLGTNELAAVALGNTWSFCTSSLILGSLNAMDTFISQSFGAKQYKFIGLTVQRAGIISIVLSVFVSILWFITKPILILIHQDPHVSALTQDYILGLLPGLWFGNALTILQKYLQGQGIMTPSIVVGVILNIFNAIFNYIFVHGLTSNGGLGVVGSSLATSLSKFISIILLLLWIKYFRLHKDTWFGFSKESLNVQGLKEYLRLGIPAGLQLSFEGWGFEILTILSGTFGPTQLDGHSIAMNFTFLTFMMPLSLSIALSVRIGQLLGSKNAAQAKRSTRIGFAITMSLMVFISLTQFLTRHVIGMIYTKEKEVQILVSHILPISALFQFFDGFQTTCQGIIRGTGKNKIGAIVNFISFYVVGLPFSCIFAFALHKEVVGLWWGLCIGLCACAIVLGSVVLRINWDAEVDKAIKRTSSFVELDQLDDEKASKIEQEEEDDFDKFNKEETPGSLSDAILYSSQQHTIKSNDKESLLHNSQEEGEEEDQVEIEIDGADYQEISLNTSNINRSTQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU84</accession>
    <name>A0A8J4PU84_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_009076</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the small GTPase superfamily. SAR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000631">
        <property type="protein sequence ID" value="KAF2069611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU84"/>
    <dbReference type="OrthoDB" id="15478at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006687">
        <property type="entry name" value="Small_GTPase_SAR1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45684">
        <property type="entry name" value="RE74312P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51422">
        <property type="entry name" value="SAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 14" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 14" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="27"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606687-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2069611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="191" mass="21862" checksum="7D195A31FF2CFF23" modified="2022-05-25" version="1">MFFFDWISWLLNWFGLANLKANIAILGLGNAGKTTLLHRFSTERLSAHPPTLRPNSHSFSIGNVNFNAHDLGGQQNMRYLWRNYIPDNKTIIIFMVDSTDFNSIGESKSEIHDLLQDQNLENSHFLILGSKIDVKSHYSKEQLIQALDLQPYFYQNPQPKNINMVMISSVTKEGFAEMGKILRDTAESLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVU8</accession>
    <name>A0A8J4PVU8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ras guanine nucleotide exchange factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_005001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000189">
        <property type="protein sequence ID" value="KAF2073684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVU8"/>
    <dbReference type="OrthoDB" id="26600at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF241">
        <property type="entry name" value="RAS GUANINE NUCLEOTIDE EXCHANGE FACTOR M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="5">
        <location>
            <begin position="237"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="4">
        <location>
            <begin position="473"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="23"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="151"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="151"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="210"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2073684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="721" mass="82566" checksum="0B66E1F7FCA47DC6" modified="2022-05-25" version="1">MWQSTSPQQNNFERVIPNFNKSLSFDSSLPPRQINGVTNNNSNTGGGGNLSSSTSALPSKVNRNTNPIPAHLQKHFLQEKNRRRGVTVATSPQQSENRRMILLGNSRPNRQRSKSLPSLNTWDYVPFEYKSTTEGQINLYQDELTEDIISTSSSSACSSPSSSEPSSPSLTRSNSSNTLLNDSTTDLTSSTPTYSGSVRERPPVPPRTYPSRSLGSSNDFTSANLTSKLNYETTDENKFIVVSGPKDQLVEYLCNKNKIDSTFGNTFILTFRYFIHPIELFNQLITKYESKVPYTSSAKVSKQYEEILERVRQNVLIVLSLWVESNFSDFLEDQYLYDKLIQFTNDSNSTFLKDSIDKQKISKIPILDLYSYLRQELLVNLSPRNSGSASSLKDKEKNQFFSYQTIIDWIMNYLSIGKNTADSIFKKLLKLKVIGSMGGAIVANVIKSQDSFFFVNNSNPKKEIYSRSFLDYSPQDIANQLTLLEFKMFQGVKMKELYYKSWTVAKTKFEQSPNVMALITMSNKIANWVATEVVTTPHPKKRVEVVKRFISIAEHCKKINNFNTLMEIISGLNNSSVSRLKETWKSLPTRYTNSFNQLQNFLKTDENWKLYRQAVKSKEVPCLPYMGLFLQDINFVEDGNSNIVEDEHNYINFKKMTLLSNILSEIQYFQKHPYYSFQTNTYIQTFIEKDIVILPDKELYAFSKFVESPTNPLNKFNKFIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW17</accession>
    <name>A0A8J4PW17_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004883</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000182">
        <property type="protein sequence ID" value="KAF2073789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW17"/>
    <dbReference type="OrthoDB" id="659430at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="329" mass="37780" checksum="5B863DF710B43198" modified="2022-05-25" version="1" fragment="single">FNGITTRINGGDLPNGLTHLTAYKHFGFSQLPESLHHLEMDRSCNNVLPPQIKHIKVPSVSLYVSQTVQSILTNDPRCNFRDVKKPNDLGRVEFNPDTILHLYSGKNFNCFIKPNSLLNNIKTITFDSNYNQPIVKGTIPNSVEYLEFGNKNQVISKEILPTSLKSLTISSFNQKLFNILPDSITELVLNMQYNPFLLFKKDDDILPNLKKLVCYYFPFVLNYLPTTICDLEFFIKPKDEKEESLKKKNNYSRKQPDIFPIELINPNITRLVLCEGMFIKNPNLIPPTIKCLTLCQYVEKGIIPSTLESLTLLNDFNDPLYTILPKIKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW27</accession>
    <name>A0A8J4PW27_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002575</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family. PCH2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000072">
        <property type="protein sequence ID" value="KAF2076113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW27"/>
    <dbReference type="OrthoDB" id="10042665at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051598">
        <property type="term" value="P:meiotic recombination checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001494">
        <property type="entry name" value="Pachytene checkpoint component Pch2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044539">
        <property type="entry name" value="Pch2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058249">
        <property type="entry name" value="Pch2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45991">
        <property type="entry name" value="PACHYTENE CHECKPOINT PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45991:SF1">
        <property type="entry name" value="PACHYTENE CHECKPOINT PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23242">
        <property type="entry name" value="AAA_lid_TRIP13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0469">Meiosis</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="196"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2076113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="443" mass="49987" checksum="1D308A669931A770" modified="2022-05-25" version="1">MDPNSSSLNNSSNNITGGGVKKIQDMKLIIEILVNDQCSISFDELRKHYLVKLYAQFELNRSQLIKQIKRHGYLSIGSADDHMAIDRVSVYCNRSIQMLELLSEQEISAYVQFERSSVFFYKQYKEGASNEELFEDVGSGEQTVSPYQQWVLPNIEFATLWDNLIYDDSVKERLLSYASTILLFSSAKVDSNIVSNNKIVLLTGPPGSGKTSLAKALSQKLAIRHSDRYAYPQLIEINSHSLFSKWFSESGKLVMKVFEKIREILDDPSCFVTLLIDEVESLAAARTAALSGSEPTDSIRVVNAFLTQLDQIKSYPNLLIVATSNLTTAIDLAFIDRADLKLFIGPPSLSARKRILKSIDKELMDKGILYANQPIDKKQQKALRSSIYTSTNGFSGRSLRKLPFLSVANYQTKSLEFPIPLAEYQIELNELIQREKEFNKVIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW53</accession>
    <name>A0A8J4PW53_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_004673</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000169">
        <property type="protein sequence ID" value="KAF2074022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW53"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF288">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="468"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="134"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="266"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="303"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="425"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="9"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="157"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="174"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="274"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="303"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="335"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="432"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2074022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1108" mass="123445" checksum="53BD8F6A575B1EAA" modified="2022-05-25" version="1">MSELDDLLMEMLAESEASKNQSASTRAQQQQQQTNSNAASSNSNVDKRKSRLSQHPQQDSNNNSGGNGSNSNSNNNNGNEGGFDYLDTLLNDMSDESIRNSIKPDNTRIKSLRLTSTFDLESTLKDLEETFMKDSNVQNKRKPSTYINRPLSPPPGTGNSNNSNSGRPLSNHNLPPPPLSPPPNDNICAVVDIELPPPPPPITSTASTTTTTTLTAAAVVDEEEEEEEVNDNEKPKLKRVSSERAVILTKEAIDAADLLDQMIESFGVPETPPKKSNSNSNSNSNKQQQESNLDQLNINLTKLTKSPNSTIRPASKLNAPVDIQVSSPKAPTPIPTLSSPPISPNSQNSDQLLDEMITLFKESNSNIKYASQQQIDAGHVVPKTSYLSQQHYPLSVEEQQIQTKVKPLPVDEEIIEKILGDQDIDNNGMVQSINSESSSTSSSLSSSSNNVISNDILEKAWYIKPVISESDKIIGSGNHGTTSRAGVHKEKRIVSKSWTFVTPQATPILFNEIEHLMTVKHPNLLPLVGASFDNNFTTFTEYVTGNNLDLVIKNLDEKNELQLIVRLAEEIASAMAFLHSLNIVHRSLHPKNILLNSDLKVYIKDFGFTLFKDETLKKKFSTPLKSQIVHSQYLAPELYNVLLGGGRGGYDTKVDVFSFGVLLWEMFARDIKLSDLKSTMVNGYTHYSRPTLDNCPFTIEKLIRLCISTDPNVRPSFSTILKILRQPLHTLQRFNKPEQAQQQSSFNNTNVDSQLETVSALANTANTNNSNLDPVKREKLSRIYQACKDLMDTSTLVNLTKAAQTIEAICKNEENHPFIVDLNFIPLILQLLENRFEDIQLICLKSLTLIIENVEIATVFRNLLGVNILIELLSNRSENLLFASLRLLAQYCQHSDAHRVESMNKGAITILINQLTHHNEAIKLQVIWCISLMLESTLVQEEFIKLNGVDILVDMFVYSTNNGFDLRVASALSKLTPLKQIQDQLNNGHYRERILKKYIQLLADVNFETLRLLGLESIASLISLKDNQDIVVAENIVPFLFEYLDVNTTNLPPQMTALKIILVLAVNSQHISYLKENQELLPIMVSLKDSPHPSIQKASEKVISLMSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW86</accession>
    <name>A0A8J4PW86_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial processing peptidase beta subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_003809</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase M16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000124">
        <property type="protein sequence ID" value="KAF2074890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW86"/>
    <dbReference type="OrthoDB" id="10251424at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000236">
        <property type="entry name" value="Mitochondrial-processing peptidase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000001">
        <property type="entry name" value="Mitochondrial-processing peptidase subunit beta, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF149">
        <property type="entry name" value="GH01077P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="10">
        <location>
            <begin position="48"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="11">
        <location>
            <begin position="201"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2074890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="472" mass="52119" checksum="D94F78C59F7A827F" modified="2022-05-25" version="1">MLNNVSKLFAVSAKNISGSRSIVSKSFDLGFLNKTPKTQITTLPNGIRVATEQSFGETASVGVWVDSGSVYENDKNNGVAHFLEHMIFKGTPTRPSPHAIESEVENMGGSLNAFTSREHSAYYMKVLKDNVPNAVDILSDIFQNSKFDSKLIEDERQTIISENNYVQSKEDEVIFDQLHAAAFQGSPLGRTILGPVENIKSISRDQILEFVSKNYTGDRLVVSAAGAVDHDALVKQVAEKFANIKAAPASQESPRAQITNDFVGSEIRVRDDSLPLVHFAVAVKGLSWDHPDYFVLELIQNMIGNWNRSISAGKNLSSNLGEIVATEGLAESFSTFFTCYKDTGLFGHYGVCQPETVDDLIAEMLKEWQRIASSVKNSEVERAKDKLIATTLMQYDGTSKICEGIGRQLLTLGRRLTPSETYLRISEITPADVKRVATSLLRDVSPAVTAIGPTGNFPEYNFVRGWTYWNRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWI3</accession>
    <name>A0A8J4PWI3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dopey N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_004505</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the DOP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000160">
        <property type="protein sequence ID" value="KAF2074187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="297643at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040314">
        <property type="entry name" value="DOP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056457">
        <property type="entry name" value="DOP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007249">
        <property type="entry name" value="DOP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056459">
        <property type="entry name" value="TPR_DOP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14042">
        <property type="entry name" value="DOPEY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14042:SF24">
        <property type="entry name" value="PROTEIN DOPEY-1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24598">
        <property type="entry name" value="DOP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04118">
        <property type="entry name" value="Dopey_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24601">
        <property type="entry name" value="TPR_DOP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DOP1 N-terminal" evidence="5">
        <location>
            <begin position="8"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="DOP1-like TPR" evidence="7">
        <location>
            <begin position="1010"/>
            <end position="1189"/>
        </location>
    </feature>
    <feature type="domain" description="DOP1-like C-terminal" evidence="6">
        <location>
            <begin position="1612"/>
            <end position="1931"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="352"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1502"/>
            <end position="1605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1522"/>
            <end position="1568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1575"/>
            <end position="1605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00046326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2074187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2071" mass="232865" checksum="4837BB5CAF85BEE5" modified="2022-05-25" version="1">MENDDTNDPKYKQFNEDLNSVLVQFSKSTEWADFVKYLSKLNKVFEKYPKSTNIQSKLIISKRLSQCLNSSLPSGCHIKALETYDILFSRIGQERLARDISIYATGLFPLFHLASTDVRVKIIHLYEKHILPLGLKLISCLNGVVSSILPGLEEGQGDAFEQAIKLLDNLCKSTSTTIFYQAIWKTLITTPYNRLAATTYLLARLPKDIDTTNQIMFLPEKDTLVVQALSTSLSDKNTLVQRNMLELITVHFSISAQVFSPEALELIVKSAIMVVTLKDMSLNRRLHSWLLGTSDDSKDGKSYDPTYFETYSKGPTISAFKNFFNSNLYNFNNSINQQQSSSLLSIFNNSNNSSSNSNNNNNNNNNNNNNSSSKINDVTLPFRILIFFFQKEEFQTIIDHILPDILRYVYYFKDGYVFSKDVIRYTDELLDSIQNQDVIWSFFEKILVESPSLDSIKLVECVLDIMKLSIQDVQSIYLPKLLVSLVRSLTGILDKQDNALLIAFTQLLLKITGKIIVDTSSPSFDSLISSIDLYQDYFKDLSMYIMKSINNSSSNSQQQQQQQQQQLLQLPTTMLSPNRKSTGLFSISSSTPTSQLSPAVSPVSSLSSGVPVPNSELFVILDLSLQMLIGLYSQFKSSFTSPTASPHLDAANEQPKWLVPILDFCNCDNPFISCLAIRGFISLINKHGDCTLSKSFRNIITPDHFAMLSKKLWTLLDPIYCSVHYKVASLFLSLRELNEEATSRVIADAMLDADLNSKIEGHQKFALLWRLAGELGSNSAPFSNTLFLMLDSLYDDQPIIRLTGHTWLADSISKAERILDPLLKILLDESTIRFNNAYQSIYDTRRVIYVFKILKAIIECDFKLFIQHVIEKPISKEILSLNEQQTMTFRESPKVVSQAKKYPNEDSYISFTHPKPQSSFSAPPTPSVASNNANNITTETSSDFLFIPTNSYIDLLVVVSLRFIQGQVPESFGDKSGNHGSFASQNDVVQICSAEFLQYLLAKTSIQHSKALEIANYIQDPILQNLAQAVSASNLVLQVHLLALLRSIVLIDSSGNLGHHSPGSPQNHIPHVSSPSALDGGGTPLSASMGSTSLSSITQSQMFLQTTVVGLIQPSTKFNIRFYWLDFITFCLPRMCHSPHLPLIVTTIVNCLRDILSSFDGRSLYDSLTSRDIIVILKSLTFIFKFSILEPITPFDKSNNEDSQTPTTRGPLGVRMFDFVKDVFTSDIDNSVSLTPLGLVRNDIFAIMSDIIHPLVKIWGQPRSTSNKISFSTDLSSDTHNKFAIQDLVVHILDPFMSKYPNQFIEAMVDIWQRTEINSNSHESVTSRKVIMEMIYSLDSVKEEPFFNAAHQILFGVHSQERTKSHKVSIHKLTMKESALYDFTSRYIEEYPIMFDGVSSPFIAFVKESLHSLNPMSFVSQLQILNIYTKKLVSEEKTKNPIRSRVNKRELQDLLPKIIDACFMISGKSFNDISAMYIPPLVSANSSSNLNGILDSPLTNSGSLSRPLSGDYPNLPQSLFQTPPSAYTSASTSITPGEESLRGRSSSGGDSILNNNNNNNESSSSTPNLQGSGGSQNYNNTTQTNNNNNTNTTQTTPGTSSQNNTNLKKEATRLKSQVSLKSLVNLSNILASLLDSILDDKERIATLLSMSLHNIVPYLKPRSDVSRENSYYAASLFASLSQFPYNLKSIKKDVMELFYDYDIFKLDIKTLEQVSKFVNQTMIHDKTALSDFTKVLSKPWNPAGPLMFVNKDTENHNRAKQLKRLSFIIWAGQEDQYLGLLPLIQEKIVESLRIPSATSLHLQVFFCLRVLLIKFNHQNLRSFWPIVLTELIDILSFSENSELILAACKFLDLALTLPSITEQFNLFEWVFFKDCFIKHSQEPPFSPFVDQIAKVSVQQQQEQHQQSNNTSNLVLTTSGIVQIENITSFRPCIMIRSISDLPNGYPEFKSFLAHLSNCVYNRHMQSTSKQVDYHFINELLCFDFCELDSSKLTLTPDILLAIEKKQQQHFETQIKANTPSIDGLSIDSNSATNSPIWTNSQKPTKTKLNRGISISGSKNLIDNNNNFSDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWP6</accession>
    <name>A0A8J4PWP6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_003520</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Rho GTPase-activating protein involved in the signal transduction pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000111">
        <property type="protein sequence ID" value="KAF2075172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWP6"/>
    <dbReference type="OrthoDB" id="19923at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050729">
        <property type="entry name" value="Rho-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176:SF0">
        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN AT 19D, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176">
        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="6">
        <location>
            <begin position="140"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="102"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="104"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="318" mass="36182" checksum="BA0F0A218EB147AC" modified="2022-05-25" version="1">MYDISYSPDVLSTSSTPSPSVSTLPPSRHSSFINHSKSTPPPVPKRPNSSLNLSIHTTIPSFPIRKPLPSIPNLNNNIHNDQSKIIPTSNNNINNSDILKQNEYENNNNNNNNNNYNYNENKNIEDNSTENNNNIDNHNNNIDSKLIYTDINSVPNIIRKCIEYITSTQSIYEEGLLRVAGSKKDIQSLREEFQSNPNPNLTQYDAYVIGDVLKQVVRERNVNQPLISYQSNKKIIDQLVDSDDDQEKIDILLQLINPLPLESKSILLMVFRLLQMVASQRQYNKMSTSNLSMIFSQTLLIPPDLVDILIKQVDNIFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX57</accession>
    <name>A0A8J4PX57_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_007506</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000405">
        <property type="protein sequence ID" value="KAF2071171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX57"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08477">
        <property type="entry name" value="Roc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="9"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="323"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="536"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="629"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="48"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="323"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="358"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="375"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="544"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="565"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="585"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="629"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="639"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="663"/>
            <end position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2071171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="707" mass="80396" checksum="7A02390CAFCA3957" modified="2022-05-25" version="1">MIKLFESLLSPRKNNSGKSGNVDDSSLPTNPLKLSGGGTSNNSERWKSPSSPSKNNSNSVESRIENNNLYNNSNSNSNRYPNIPIECTIKILVLGGAKVGKTSIIRRYIQECFTTAYIKTIGIDLSTLIVRPKNENRKYLLDVEQLLKDFRQQQDDSGGDLSMEDMGDYFDEEPSSFLIHFWDISHSEIKGNHFNSLFNDVSGAILVFDSTNANSILAMDEWKTILQYKQLLTTKKIPIALLANKSDTGESIISATDLEFYCKNCNFDIWKYTSCKKDIGIQESIDQLLSKILDQKVSQFEERKSLKIISIYDQQKLLKQQQQQQQQQQQYPMSQSINTSGSSSNSNSSQKRRKDRPNSQSVPNSNNSPQNSPNKQHHHQQQHSHHHHQPTSPISQIEISKINELLDTTNQHYGKFQKDIETLMVQSSGSNRYNDLIQLERQRNNEYVKICNNLKKLIHPNVFNHHHHHHHHSKNNNNKFTRESLQFVLEENIKKWDSLINNLLLEKSLKLQQQKQQQQSCNNSDQKNSVSLVIKKENNNSSLDQDEEDFIEEDEDKKKYKRKNNNYSSSSGRKATSPHSSSHSLKGHHHHHGGHHHHHHSSNPKYKLGFGFRNNGKNLSSSITISQNNYSIDTDNPASTSSSLTSSSRSISPNSSPPPSPPNSFTNSFTNSFSSSPSVLPSSYHLNVTMIPDVKFSTSSEFVNSFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXJ2</accession>
    <name>A0A8J4PXJ2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="1">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_003321</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="1"/>
    <dbReference type="EMBL" id="AJWJ01000102">
        <property type="protein sequence ID" value="KAF2075397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="77911at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056598">
        <property type="entry name" value="FKBP-15_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44927">
        <property type="entry name" value="FK506-BINDING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44927:SF1">
        <property type="entry name" value="FK506-BINDING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23649">
        <property type="entry name" value="FKBP15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="4">
        <location>
            <begin position="176"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="266"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="316"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="457"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="520"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1034"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1209"/>
            <end position="1586"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="613"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="875"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="274"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="322"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="457"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="524"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1042"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1072"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1089"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1137"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1224"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1237"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1275"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1327"/>
            <end position="1342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1367"/>
            <end position="1397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1399"/>
            <end position="1414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1427"/>
            <end position="1438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1441"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1472"/>
            <end position="1485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1492"/>
            <end position="1502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1518"/>
            <end position="1534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2075397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1586" mass="174614" checksum="0C458944CCB8699E" modified="2022-05-25" version="1">MNFSDNEEYSNRNNASSQKLGSLFKDSNTMSGNQSLQYTNSKQQSSAASNAPEVIASYPIHLFKLEGGNYSSRGQVATAVVGYKSTGKFQFLVYDNSKTYIVTINISPNFNYTVNETYGYFVDQNNQSWSMSFNSVEDSHKYATTVAIVKCASGSYQSISTVDLGTGAKTRALGAGDRATIKYSGWIENNQKLGSLFEANGEAKFVIGDGSVIKGWDLGVSGMKKGVKRIVVVPSDFAYGKKGNKNVPGGANVIFEIDVIATKKREGKEDPSLPNLSGQATAAPQASLPNTLFNNSPAPAADDRANLMQRVAKLGRATGFVSPSPSDSESESSRNYNNNQNNNNNQNNNNNNQNNQNNNNNYNNNNQNNNGNNNNNNYNNGNNNNGNNYNNNGNNNNNGYNNNNNNNNNNGNNFNNGNNNNNYNNGNNFNNGNNFNNNNNNFGMNQMNNMNPMNPMNPMNPMNQMNPMNQMNPMNPISPYGQPINPMNPYANPLLSPTQTGIYSSTSGALVPIIPQQPSIIVQSPPPAPPPTPAPAPPPPAPVVVQQPAAPAQTASETVQVLVEERQFKVELKDSIKTFSDKLDRVAAKFETLNEPIVKGEIPISGLLLVQTLNKLVEDNERLIKESGEKENRMNALKENMAALHKKNEQFLEENTKMMESRNEAITDNVSQLRKQIIDLQKQKTAIEDQLTDKEMSFTKSQSTISQLKREINTTTEELNNARDEIIKIQQDQDSAKKTEREAALTMRALKKSLLDEKNARKSESEKIATLEDELKDAQESLAQAEKSNEESIKKVKLDMLKLKKRNDDKVAQLNLEITSLKDTLETERNAMGSVTAMIESAKQEVEAKLNLTILKKEEEIGNLVLSVEAEKSSIESLKKLHQSELEQLNALRTKIEKQLSEEVENVSKSYNKGFSDGIESTKHESDMVSSLQQQIQQIQSKYDQSLLDLDKANSAAAVAAANAAAALASAQSASTTATTVVSAGSVDIAKEVKSLMQKVFAAVYEEFEEEEVYSAEDIVNSFKGIIKQTTTEFLAPKPKEDEVEEGEEEEEEEVEEEEEEEEEQIVEEPVVEAVPSEPVTAVVPPVVDESESEEEQEQPEEKVQVVDPLSTPEVQEETPKSEPIAVVVPPVVKETSDDDDDEFDDDDYADIDEEQLKNIDAEIEQMKLDMENEEKAEAETTAVDPLTTTVVNPQITDAVVNPQITDVVVEDPLAVTEEKEQSESESEEESKEEENPLTTTVVNPQITDAVVNPQITDVVVEDPLAVTEEKEPVVEESESEEEQVIAEPPQIDDESEPEVEEPKEEAIETPINSQITDVVGEESEKEEEKQESEKEDVKVESEQEEEEEKVEAPPSLSDDDEEEAVEEKVEVPFIPEDKGMIDDKDIFSKDEDKSDSFENIDDPPSLEEKEFEEVPSITPNEASSGEEDKPSLEDEPKPATPTKSSSKPATAKKNFFDFDDSDPFATPAGDVESKKESTPKKDLFSDSLFGDAPSTPTTATKTPKKDPFADDGDDDLFSFSKSAASESTPSSNAFDSDSLFGGASLKPKSTPNKPKQDFSGLFGSDPTASPLDETEKAQEEFTLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXJ4</accession>
    <name>A0A8J4PXJ4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Regulator of chromosome condensation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_003074</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000092">
        <property type="protein sequence ID" value="KAF2075606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10256179at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07307">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051553">
        <property type="entry name" value="Ran_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023152">
        <property type="entry name" value="RasGAP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982:SF1">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00509">
        <property type="entry name" value="RAS_GTPASE_ACTIV_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="588"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="643"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="698"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="750"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="850"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="900"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="5">
        <location>
            <begin position="954"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GAP" evidence="6">
        <location>
            <begin position="1590"/>
            <end position="1782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1432"/>
            <end position="1497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1508"/>
            <end position="1527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="27"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="92"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="172"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="265"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="281"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="360"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="479"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="495"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="530"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="551"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1432"/>
            <end position="1488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1510"/>
            <end position="1524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1882" mass="204807" checksum="BC35999A48BB22E5" modified="2022-05-25" version="1">MSYNPPPTIPTSKPSTGGGRPLPNPPSATSTPTNVNSSPSISPSTSSTNLNGTNNVQPPSPIGSMKSPPPPLKPRAKTSSLGNGGGSMGALPIPNNSNSNNSNSINPTTITNTGSGSTPTQAPPMLHTPPPLNSTTPQKSPSPPSLPKRDFPNGGSNGNLLASNTKTPSSGNLVSGITNSSSSGNLSSNLNNSSSSVNSNTSGNISSSSGSVGTVATSMPPPTTPPTTSSINISNTNSNNNNTNNNNTVYSSSAPVVNSPLVNFKTPSTPSSSSTSTTPSPSKPEPLQLPPTIPPSQSTEEICTPQKKRNSLGDLNTEQLNKMIKKEQQQQQQQQDDDDNQDDKKKKGKMFPHQWAMGKLSEKEMKKEEKERKELEKREEKERRDIEKREKKEKKEREKAEKKGDKDKDKEKDNKEKEKDKTDRPEKSLSRTSSSFAAKLKHATLRGGSKADFDDDSGSNHSSPSVSPALMSNRERASVTVSTPSGTPINPSPLSINNTSPTISVTSTSTPQHNTSSSSLSVGGHGNNNGGSNISISSSTSSNEDLIATGSSPSTPLLSSSPTMHGSNSPSNTSSTSLRSAIHLDTSRGLMTWGSASNSKLGFKLATKDLSQPTPERLPNFNIADICSISSGSYYSAALTEGGDVYMWGRGAVKNPPIPTLGFNQIEDQLLPIKIESLSEIVVISIGFYHSAAVKANGELLTWGCGESGQLGHGDSNNQIEPKVISALSTQWITQVQCGEKHTICLTKNGKVYSWGTSEYGQLGHGDTNKYCAPMLIQALDKYNVIQIASGSTHCAVLTNSKEVLVFGNGAAIGSATIVSIPTLVPSLKYLHIDKLACGHYSTAAITECGDVYTWGSGQELGHGTNQSESRPQLVEALRNQSIRQISCGGRHTAFLTDSGRIFTCGKDSFGQLGHNEGDQNKPKKIESLTKSTFISISCGENHNIALFDTTRSFRDKFCWKLLETQRTYVRTLDTIQTIFLNPLRVRDRDQLPESMRHLPYIFLSEDEIKTIFGPIEKLYALNSTVLHIMNKRFQTWSNKKKVGDVLLNHFKHSEELFNQYVENIPLALTCLDNLCKKQNSPVIGYLNACEKLANEKKKVDTDDDSKEFTLRSLLMEPLLVISRYHKSINGMVKYTKTTHIDYAGLVDLDGIMEAKVNKVQSMVNIIQNPSTLQSYATTHVTASTSDDSSAFLKEYDLKIESLQNFRKTCSKVIKSSKKYFEVEPESFKEQGHFSENLSFIKTSFDGSVDPELASSIEHFSSSIKKIGYLRSELSTRTTSAFSQPLSTVSDELEAFLPLIVEMRKRVFESHTEYESAVNKLYSLAKNLQPTDKKMIEAEKEVASLKKVLDKALVDFDSIFKEGVSIQTKSLKLFYACMKAQQEYFERGLQRFQAMKPSFDALDTFFRQQSRAQWSQSIHSVLTSTFSSSAAEKSSSNHSASPSPFTSSSASGTAASGNSNFTPSSAPSPLVLSAPAANSSSGAAGQISPSPSNETILATPKDSFALEGGETTTAIPSTPSKSTPAQPPMDPHAIMVELEKEDWSYLLDPPSANEFTETFIEDQYNQLVEILASPSLQVVQCVVSPAQVEEQSRTIESISRIFESFNKIRSIIQTGIQSEVQSTANPSTLFRSNTTATKLMTAFTKLKGMPYLQKYITPLVKEIIENPFGYEVDPSKINEGSEELMTNMMNLIQISEKFTDAIIDSLGGLPISLREISKLLQSEVIKKFPDNKHSSVGGFIFLRFLCPAIIAPHTAGLVDEAPGPEATRALVLIGKVLQNLANGIEFGQKESFMIPVNRFIIGNVNRLNSYFDRLTDVPDHKGDYISSNSKEEVQKDIRNIHILIVKNLSKVLKQLALYRQKDIIGHLSKTLVHLGDPQALYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYA3</accession>
    <name>A0A8J4PYA3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ADP-ribosylhydrolase ARH1</fullName>
            <ecNumber evidence="4">3.2.2.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ADP-ribose-L-arginine cleaving enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">[Protein ADP-ribosylarginine] hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_002773</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Specifically acts as an arginine mono-ADP-ribosylhydrolase by mediating the removal of mono-ADP-ribose attached to arginine residues on proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ADP-ribosylglycohydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.19" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000080">
        <property type="protein sequence ID" value="KAF2075925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYA3"/>
    <dbReference type="OrthoDB" id="10250509at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003875">
        <property type="term" value="F:ADP-ribosylarginine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4080.10">
        <property type="entry name" value="ADP-ribosylation/Crystallin J1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050792">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005502">
        <property type="entry name" value="Ribosyl_crysJ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036705">
        <property type="entry name" value="Ribosyl_crysJ1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222">
        <property type="entry name" value="ADP-RIBOSYLGLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222:SF26">
        <property type="entry name" value="ADP-RIBOSYLHYDROLASE ARH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03747">
        <property type="entry name" value="ADP_ribosyl_GH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101478">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00049582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR605502-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2075925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="376" mass="42294" checksum="886894C9AAC4EF66" modified="2022-05-25" version="1">MEKRYEATMIMSAVGDAMGYKNGSWEFQMSQNTITKEYERFNGLENIVIDKKNWRLSDDTIMHMATAIAITRPENTDFNSICEQIALAYIRCMEDMGGRAPGRQTMNSVGLMTKGFSSKEPLKWNEIPYANGGGGCGGAMRSMCIGLKYWNESQIDDLIALSIESGRITHNHPVGFLGAMVSALFTSFAIQNVDPKLWGTKLMCEGLPKAKEYLKKTENDTNRKWENYVRGWNYFEDAWKAYLKLRKIPLSLDDALSAEQPTYPEFPETYGYMERMAFYQSVSFDGWGGSSGHDSVIISYDALLGAGSDWKEMVERAIIHAGDNDSTGAIGCAWWGALYGMESVPLNNYDKIEYKPIIVGLSKAIFKSLEIPHDQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYB8</accession>
    <name>A0A8J4PYB8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_004398</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000154">
        <property type="protein sequence ID" value="KAF2074304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYB8"/>
    <dbReference type="OrthoDB" id="10020990at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34500:SF1">
        <property type="entry name" value="DDE TNP4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34500">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="DDE Tnp4" evidence="5">
        <location>
            <begin position="97"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="272"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="282"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2074304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="296" mass="34233" checksum="F07723F1280D2360" modified="2022-05-25" version="1">MQLSLGIKPETIDHLYSLINNEGFNKDYIIWVFLYFHTYMPIGQLAVICGVSYRTMISHINGTISILYEKVKYLINNIEPSVGTINYNGVTFSMAAIDSMCVLVNKPQDDVVQKTLYSKKHGDHLWKFEFIVGLDGKLLYISPAFEGSRHDKAIQDITRSAQVFPYPIFGDKGYKGATNIASPVKKKSDHLTPDEKLYNNFISPQRVIIDNFFNYIKRFKILSTHFRSKDQKEKLEKIIFIIAYAINFQLNFNSNNNNNIIYFNPISTHNRNKNNNNNNNNNDHDNNNRNDDENIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYF5</accession>
    <name>A0A8J4PYF5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BP74 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002968</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000088">
        <property type="protein sequence ID" value="KAF2075725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYF5"/>
    <dbReference type="OrthoDB" id="14772at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056422">
        <property type="entry name" value="BP74_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053344">
        <property type="entry name" value="cAMP-inducible_BP74-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35883:SF1">
        <property type="entry name" value="CALMODULIN-BINDING PROTEIN CAM-BP15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35883">
        <property type="entry name" value="CYCLIC AMP-INDUCIBLE PROTEIN BP74-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23621">
        <property type="entry name" value="BP74_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035144823" description="BP74 N-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="BP74 N-terminal" evidence="2">
        <location>
            <begin position="23"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="135" mass="15538" checksum="2C59A54A3AF7FD29" modified="2022-05-25" version="1">MNKLLIGTLIVFVTLLAVASCDEAYFGFKTHANEHEFVFKLTDVKKIEHARKILSGDEKNEVHVLGRFKKTKKDYNPHYNFHLEPENTSFFEMAIEVCDASLEYTEEHLDEACGAFLPGCMYCPWSSKLTREVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ27</accession>
    <name>A0A8J4PZ27_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Proteasome assembly chaperone 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003060</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PSMG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000091">
        <property type="protein sequence ID" value="KAF2075637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ27"/>
    <dbReference type="OrthoDB" id="17536at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080129">
        <property type="term" value="P:proteasome core complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016565">
        <property type="entry name" value="Proteasome_assmbl_chp_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15069">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15069:SF1">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16094">
        <property type="entry name" value="PAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="268" mass="30971" checksum="0CC990BE05F8B654" modified="2022-05-25" version="1">MFGFEIQPASSRNWEIEEEEEELTEFEPLPSPVIKFNLSINENDLVQKNLVISTKDIGSLFIQSTFNVGQPNYQIEEIGEITIQDIVPLKPNSKTSILNNNKCIIYRHKEDSSYIFVVCQYDVPADRAVQFSELVLEHIKNPKQVIILDKLLNSHFISQNYEYPLPPFVRALYTSSFKNSYSLVPLESPNIIQNVTASFLTLCEIRNISACSILSFYESYVNIESIQSYESIFKSIFPELKITHTNIKDHYQTVLNQINYRDDKGIYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZM1</accession>
    <name>A0A8J4PZM1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tetratricopeptide-like helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003509</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000110">
        <property type="protein sequence ID" value="KAF2075204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZM1"/>
    <dbReference type="OrthoDB" id="43460at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019412">
        <property type="entry name" value="IML2/TPR_39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31859:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31859">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 39 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10300">
        <property type="entry name" value="Iml2-TPR_39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2075204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="514" mass="58977" checksum="5F7AAF15DAC907C6" modified="2022-05-25" version="1">MNSEQIEALDQAMHHFWNFNLIALPLFGLLHSDIAFIKAVLTETKEDYAEASKRISECKKLARALQLSQKPKYLQQEKEEKKNGSSNGAGTESSAFTKAEYLISKLIYAETMVMKGVIEFKAQNVLKAGIALRKSWKQYYQAFEIAQTLPTTFPIYNHIMSLAYYGVGIFHFLVSVVPPQYMMLVEGIGFKGNRMEGLKELKVSSDSNGIRSVMSKIVIVLLDVFFFEDYKKPEPLLQSLVDAYPNGALVHYMSGAILRKQGKIESSTESYQKAFETSSQLKQLQLFIESELGYNEFLNLNWSKAEVYLNKFMNETTSSGFKAFIAYQLASCYEFQGNNQKAQEVMKTISSYVRKGFDFDEFSGRKATKFLKNKCYREFEKTYIIAALHNESHRFEQSSKVLQDYLDTKRSEMTPDDLACAHYLLGFNNQSLGKKEDARKYYTTALTFEKQITTEHFIIPYSNLGLGEISFIEEKKSDCKTFIKKSKSYTSNAYDFPSVLDWRARKCLQQIGEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZQ5</accession>
    <name>A0A8J4PZQ5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001710</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000042">
        <property type="protein sequence ID" value="KAF2077001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZQ5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035300334" evidence="1">
        <location>
            <begin position="20"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="313" mass="34641" checksum="71E34258676EDE38" modified="2022-05-25" version="1">MNTFTTLLILSTVLSCVYGQTVYTLNRNAVASMQWNTNTFQQFNLPEQLFEVHQISGANNGAVYSLISNYSNYFSLVAIDSNSGNIKQVEGMYSTSEFPTAQFTNVFYDSNLNSAALLFEGNDESSDSQSVDGPTDLALLVYRFQENQIQSTWLGPLNGIVMGSYDQSNQLYYIANVYQTGSYKVSVYNTTTGSSVRSTTIYAPKISPNSLYYVLAANGNLFSFVNIGIKVEIYYLNIENSSQELVGIISTGFWQAIDAFSAFFDPATSSVYFSVYSDSQSSIFQLSFSAMSINTVYAQTAVQLKGDLVYVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZR3</accession>
    <name>A0A8J4PZR3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_000419</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000008">
        <property type="protein sequence ID" value="KAF2078228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZR3"/>
    <dbReference type="OrthoDB" id="418595at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04623">
        <property type="entry name" value="CBS_pair_bac_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044725">
        <property type="entry name" value="CBSX3_CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="113"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="180"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2078228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="256" mass="28627" checksum="C09CE37C9BC9E22E" modified="2022-05-25" version="1">MINNINRLLVSSSRQGGRIIPLINNSGSNNSSIGSFGNSRFYSSSPDKSNSTVHLTKNEFYYHYTPKDQSGYMKKSLYDYDPLAKDHQLIKTAESEYFVFGDKTVGEILKSKENKELLTINDSDTIYQAIEKMENHGVGALLTVDKETGSLVGIITERDYLGKCALRGLSSKEAKVSDIMTRGVKTISPNLCVVEVLAIMTKERFRHIPVTDESGSKVIGLVSITDLIKVLQKNQKDTIKYLREFLNDSTGYSYKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q077</accession>
    <name>A0A8J4Q077_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_002365</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000065">
        <property type="protein sequence ID" value="KAF2076309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q077"/>
    <dbReference type="OrthoDB" id="1049195at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12418">
        <property type="entry name" value="RRM_Aly_REF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051229">
        <property type="entry name" value="ALYREF_mRNA_export"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19965">
        <property type="entry name" value="RNA AND EXPORT FACTOR BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19965:SF35">
        <property type="entry name" value="RNA ANNEALING PROTEIN YRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="213"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="15"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="46"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="64"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2076309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="298" mass="32830" checksum="FEE2D4D0F9C4E205" modified="2022-05-25" version="1">MDVNMSLEQIIKKNHIKRGGRGGAPTTVKDKKSAPPTTITITNNHTKPKRSSLPLNRRRPISGPKSTTTTTTTSTPLLTPSSPPIPSLKITIQNQSPKPSTTSGPSRKSTESSPLLSARPKIRKSFANESPLKRRTTDRSNLVIRPRTTGGGGGRHHQMSHDYSDDEDFGKGSSLFERRSSYSPSSPSHHSHHHQRPLSPVTSRKPIQLHTGSRLNISNLHFGIIESDLLVLFKKFGDVKSIRINYDSSGRSIGTGYCIFARHADALKAMEMYNRIALDGKLLHITLGPSLQEDVRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0G3</accession>
    <name>A0A8J4Q0G3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002262</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000060">
        <property type="protein sequence ID" value="KAF2076459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0G3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="590"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="592"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1033" mass="119104" checksum="D373165CE1924A20" modified="2022-05-25" version="1">MTVINGSLKWITPSLHKENVLEELFAYQLTSDRFGNSGISNDIPLDIRQFIIIITESDLHRLDNINLSEISIPTHITDLHLVFDFKFTNPLYKVRESDIVKTGQSLKEREEQDRRFNQLKLKFNDFNIPSNIISLTISNKMDCDDDGIHGFLLGFGTGDHLIVPKSVEKLTCYPWQGTGDHLIVPKSVEKLTLPWITGVRCFPPPVKHLEFVNLSRSRDNEEILRSIAVTVFPSNFCVTFGFLPAIKYHMLEKVALLSGKNETVDWLPDVQHLVLDNGCLMDQLKDLPNSLKYLNIENGYVQILDLPDQLHHLEIYIGEWANIYNFIPRQITHLKINIFDDDHMADNISSAIYLPSTLVHVDIDPHYSNFIKVIDAKEYYKPIVSNSNLKILHTSDSFNQFIRPNSLPQALQEFRIGKSYTKTILKGTIPQSVLKLIIRCDLGKCIIQDYFPNVTSIDFNHFGYFENINLESITEMVGRKDCGFPNNKTLKKLMIVGDLTQSPKFDNLSSMYPNLEELHIKNSQLSPLNFPLSLTNLRFHGLDVPNSIQIFDITSKHKIDLSKIPKSVKELSLDFENYGSLLLKENFHIRDTNNNNNNSDSSTTDNNNSSNNNNNNQFFNIFRNIYLKKMIMESLVNADNNRFQQLRKRERGNHILYIHTPTTVMEEHLTPNITKVIFKQNSLKELEKPLPSYLSRYLSDKYHVYEKGVEVPTDTDILIWPFNQVIPQGIIPFGVRSIYFNRDFNQIILPNTLPDSVLEIRFGLNPYTQSSLYHIGPNVDHLAFGISSKTKVQDFILPNIRFIKINNSKLMFLKMMRALKRPKRQLIVPDSVQRISSNQSKSSFQTATTQTFLQKFPLIYFKSKEEEEDLAKEKLDPSIILNLRIGCNQFIQPHLLDFLTILKFGKSFNQVLSKGLLPSSITKLTFKRGFYHQDLLDWIPPSVTDLVIGRELDQTELNNDKDHDVFPIEKLSNTSVCKLMLGDREVIESPRLIPINIKQIRLCKCKTIEPIPDHIEALYLGSHHNIPLRNYFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0L7</accession>
    <name>A0A8J4Q0L7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000985</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000021">
        <property type="protein sequence ID" value="KAF2077738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0L7"/>
    <dbReference type="OrthoDB" id="7600006at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF178">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="567" mass="63981" checksum="8D272798F92340AD" modified="2022-05-25" version="1">MFRSTPLRFELFQYNAECRFMDIDSDVVDITLVINGNGEVPDQLRPVIRQINIHSDFIFVFIPNFIPNSVTSILIFDDDGSAFDPYDHRIHFDQVLPDSVTYLRFPILKDVSWRTNNYTLPKNLIPLTRNIKELDLGTMALDGKVEDFPPCLTNLTLSQQYSPSKAITKLKDLMYLQRLSINFNRLYKIDSAFFPKTLESLTMTIYQEIYNGLLPPNLVELKLINPEPIEMGSFPASLTKLKLFKFNQPLVPGFLPPQLKSLSLELNSQILPNNLQVLKTGDSFDQQLDVNVLPSSLTSLIFGPLFSFPIKRGVLPESITDLNLGSSFNHEIGVGVLPSKLLRLDLGYDYNQPIKANTLPPKLSVLFISPFYNQPLEPNVLPPSIKQLALGQLFDYPLEQGSLPPSLTYLVLPLNYNQPIKPNVIPNTVTHLGFSHSSNPAPDLHIIPTGVKTLIFSNINYNSPIEKGFIPSTVTKISINFSYTQSLIPPNTLPNSIKTLTFYRKPKERLLPSVLPSSLTRLNLYYSDNNDTSQLITPESLPITLKSLCVTPPFLSKNLFKFPIIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0X1</accession>
    <name>A0A8J4Q0X1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002907</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LAMTOR3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000086">
        <property type="protein sequence ID" value="KAF2075774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0X1"/>
    <dbReference type="OrthoDB" id="343907at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071986">
        <property type="term" value="C:Ragulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032008">
        <property type="term" value="P:positive regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015019">
        <property type="entry name" value="LAMTOR3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13378:SF1">
        <property type="entry name" value="RAGULATOR COMPLEX PROTEIN LAMTOR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13378">
        <property type="entry name" value="REGULATOR COMPLEX PROTEIN LAMTOR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08923">
        <property type="entry name" value="MAPKK1_Int"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01278">
        <property type="entry name" value="MAPKK1_Int"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103196">
        <property type="entry name" value="Roadblock/LC7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="131" mass="14236" checksum="7D5496A432655BF0" modified="2022-05-25" version="1">MDKVEEFLATLKSTILSTAPDSSLILSDRDGFVLGKLIDKNDSADKIIENSALSIFSTSTDQASKLYSGKNKAIVSYFEDRIVVHINVTNVILSIVTDTDSNVGVILGAQNDIVRSLNNLSNSIQIDLQDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1F5</accession>
    <name>A0A8J4Q1F5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DDT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_000689</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000013">
        <property type="protein sequence ID" value="KAF2078051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1F5"/>
    <dbReference type="OrthoDB" id="332390at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018501">
        <property type="entry name" value="DDT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053271">
        <property type="entry name" value="DDT_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028942">
        <property type="entry name" value="WHIM1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028941">
        <property type="entry name" value="WHIM2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013136">
        <property type="entry name" value="WSTF_Acf1_Cbp146"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15546">
        <property type="entry name" value="BROMODOMAIN ADJACENT TO ZINC FINGER DOMAIN, 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15546:SF2">
        <property type="entry name" value="DDT DOMAIN-CONTAINING PROTEIN DDB_G0282237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02791">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10537">
        <property type="entry name" value="WAC_Acf1_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15612">
        <property type="entry name" value="WHIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15613">
        <property type="entry name" value="WSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00571">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50827">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51136">
        <property type="entry name" value="WAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WAC" evidence="7">
        <location>
            <begin position="20"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="DDT" evidence="6">
        <location>
            <begin position="380"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="139"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="297"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="525"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="643"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="794"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="139"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="180"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="317"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="349"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="534"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2078051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="933" mass="108659" checksum="00293A4865CC8175" modified="2022-05-25" version="1">MPLLGKDPFELSKTRVKPGSEYFVIRFTKEHFKRYKEYIERFELYRQKIWTCSITGKQNLSFEEALLSEKQASDKVSKISEILLPHVLTMIQYTESTLEELTNNLYEFLMMNYYPGEIVSFKKGGTKYVGRVIESYESNDNEMAESEDEEDAATTAKQSENESGAEESAKKKRSSKYSDDESEEEEEEEEEKKKSTTTTTTTTTTTTSTGNPKGKSSRKYLDPRKRLKSSKLMYKIDAHSKTYEVSVKDIMRREPPIFKPVVKETIKCVAKKTKHLGGYWEVDPELMKKHNLESLPVPSHLRKEVEQHLKRKRGEVDSEPDTDSSSDEETALTADGQPRQRKKPKFPIEDLDIDRDGQDEAGSPELDKRPIASSNFICLPTSYGELLSIWHFLNQFEKVLALSPFSLEDLEAALQHTSETSLLVEAHTRLLKTIFTLPSYSTGTPKKTFGSKTLSDKNWQATLKLFFQHETKRLAIEEKKKQYKLKQLEEQNTRMLHLANELPGEGNEDEDQDISSLFDENGDLIMKDKKSKDGDDEEGSQDEDEEEEEEEEESEYESEEEIEESNIIDSMDPNLISRVIRKKNYFRLDVDEKIYILAYLCRQAISSDRIRKQLDQMTDVATEIKQEKREIILEERKIKKEEVETKNNNNSKDKNSKDKQQEQENEELEKLAKMEENLDQRLVKYSTRLESMGKDRNFNKYWYWAQLPGRLYIEVSETGNWEYYSSKQEFDDLLSYLDSRGIRERKLLTNLKQRYNSISNDLEAKTQKILKQITFETKRSARIKALYALYFNNNNNNNNNNNNNNNNNNNNNNNNSNSNSNSNSNSNSSNNNNNDSNNLFEKLKVRDNIIKLTRTFIFLIKIFSTTEGMKMWVTTESVTLLLENTLVILNGPTLLVNNFFEIRSPELWVSWKYFRFTITLSPTLYSVTYLPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2D4</accession>
    <name>A0A8J4Q2D4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001991</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000051">
        <property type="protein sequence ID" value="KAF2076734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2D4"/>
    <dbReference type="OrthoDB" id="10263782at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009235">
        <property type="term" value="P:cobalamin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019362">
        <property type="entry name" value="MMADHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13192:SF3">
        <property type="entry name" value="COBALAMIN TRAFFICKING PROTEIN CBLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13192">
        <property type="entry name" value="MY011 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10229">
        <property type="entry name" value="MMADHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="183" mass="21061" checksum="B986D2B42414D5CE" modified="2022-05-25" version="1">MKLNEAEFIESLNVEYTIKKCTSTPLINNFKQMFPNDLSEIKVQTDDDTIDENNNNTLYMIISWIPTKLPMSGFSSATEDEREEKSLMFIQNATKICKYIQEKNYWCDFIDPLTGVPHIKRENANSVFVPTESVPYMNIEIIDVGCCQVMVHPKFKVRSFFSVIVSTAPFKILDEAIHATDQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2V9</accession>
    <name>A0A8J4Q2V9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000954</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000020">
        <property type="protein sequence ID" value="KAF2077767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="659430at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="844"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="878"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="844"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="880"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1405" mass="160080" checksum="7D50D2F845484DAA" modified="2022-05-25" version="1" fragment="single">VGKQQQPNFKKRKQDNDNVDDDFDNHSDDSEIEIEQPHNSNTNTTTTTSTTIPNNNQHSILNRDDIRTFTLEKGYIKSNITKLEVQLENPIYSDVILGDFIPDSVTHLEIPILYAFLCPLIPKSVKSLKLKSQIPVYDGAFRMIPPHITDLSVIIVGDWTLPLPDISPTVKKITINKLYPFANLTSISYMENAFDIALHYNMDEDLSIVSSMRSNTINPVIQQLMKKDNVFVKCEGSGPIPQTTKHLTRMCRDTDPLPFGITQLICCQDRAIPPGELPESLTKITFINLNQCLSNVHLPQSLKYLLIPAMDQLIYRNTHIPKSVTHLGVDLHSSVHEGIVRNSWLYKKGYLPESVRFIIINIANLKPSQISDIILPSSILYFDLQVEEVYIDRLELFYKKIKFIDAQEYYHNAPEFVQTSTLETGNISKIDFGKHFNQLIKPGQLSSNITDLTFGQSYLQSIDEGVIPSSVDKLTLECKESIGAVSIPYPISSLSIPAIFLPDNPLADKTSIKDLSIDGKLNQVERMVNLDDEDDQSLDELDQTPSIPILIEQLPFLSLEKLCLKNVKNIGSETMTEYSFPHLQELKIDTIYPDTQRIYVDFLSKVMVNLKSLFLYGNFKSHLKNIDFPSTKLESLDANEFFHINPNQIPSSMKNLSIPINEKNFNLIQSKNFVQPKIKNDNQDFLKKRFEINSRDNELFFQIWRNSYLKPMVFSQLQDMMAHSFIYHDYHSKKEDIERDGSRFKNLSIFTDTVITGLDKIPSNVIAWYFSNRAQTLPSIIPETVKKTACGYYPNIQIPSWITHFSLYYGDGKIVKKGFLPESITHLELDTITIASQYEEFGFFDDDNEDQDDESSDYHNDFENDDIRESQRVGVLFDEANSDDEDHDDGDEQRKERDRLDDEDEAQNDEIEAYYLTLKEALPSKLRVLNIFNLKFGVCNIIPPTVEILQIRDPKFKLVDKSFKNLPSTIKKIKIPKNCFQNVKKIPSFLSTRLDGNYQVYEKNATIGKKTSHLIINGPLVIKKGLIPDNVNTIIFGHSFNQPIPHLPSSITEIKFGYHFNQDLSTISFPPNLKYLSFGDSFDKPLDSLPAGLKFLQFQVLNPHSAQSLASIPDSVDHLSIKHAFLDSDSDDEDDNDDYSPPSNPLLVIPDCIKFLKLGVYIENVKLPLNLKSITCHHSCLGENKLKTSEKKVRETIDTTLSLENIDPSIKLNINTTGSFDRLINPNSLIDNIKSISFGTNFNQVLPKNSLPPSVEFLKFGFCYDQQFQIGNISSSVTQLELGTEYDKSLKGILPNGLKKLIIKSGFYHKDMLDWLPKSLEYLELGNDEIVPSPQPFPIEKLPTNITTLILNDSVIISSPSKIPKTIKHLKLCKCKTWDYLPIDSLESLTLGEYNYPLNMIFKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q324</accession>
    <name>A0A8J4Q324_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Rab GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_000745</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000015">
        <property type="protein sequence ID" value="KAF2077944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q324"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01867">
        <property type="entry name" value="Rab8_Rab10_Rab13_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000363">
        <property type="entry name" value="Secretion related GTPase srgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="185"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2077944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="212" mass="23643" checksum="FF605AC024866324" modified="2022-05-25" version="1">MSTGPATASNKSAAYDYLIKLLLIGDSGVGKSCLLLRFSEDSFTPSFITTIGIDFKIRTIELEGKRIKLQIWDTAGQERFRTITTAYYRGAMGILLVYDVTDEKSFGNIRNWIRNIEQHATDSVNKMLIGNKCDMAEKKVVDSARGKSLADEYGIKFLETSAKNSINVEEAFISLAKDIKKRMIDTPNEPPQPHNTTNITQTNNGKKSTCCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3F4</accession>
    <name>A0A8J4Q3F4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Hemolysin III</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_001291</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000029">
        <property type="protein sequence ID" value="KAF2077442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3F4"/>
    <dbReference type="OrthoDB" id="15997at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007778">
        <property type="entry name" value="Dict_REP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF3">
        <property type="entry name" value="LD03007P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05086">
        <property type="entry name" value="Dicty_REP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="173"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="297"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="148"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="301"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2077442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="407" mass="46132" checksum="80EEBD2128186E22" modified="2022-05-25" version="1">MESHNKTLSEIIIGPEVQTQSEEILNALSHGLGLLICIPAVFFLLSLARMRGSKWHFLSCSIYGISLMTMYFCSTIYHSAGIFSNINPRYRDILKDLDHCAIFLLIAGTYTPLTLINLVYNNLPSFVGIIQTTFHLKNKNDQKKKDNSTTTTNNNNNDDGVVLKKNTPNVVKVGWIMFIVVWMMCVLGVGSKLILGAAGIPEFISNGFYLTMGWVSIIGIKDLIQHLPKKSIKLLVFGGLTYTSGVAFLVWETAPFNHAIWHLFVSAGTFFHYFCILESLVPSINIKKDLVEDNHSHNIQNNNNNNSTTTTTTTTTTTTKTVIRNRVPTNNNNIVNNNNNNKGTNHHPYYRDLHLISNNHSSVILEHFEYAEKHIKLDYFGSGRRRAFIVKYMNTLYKMFSTLHRDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q467</accession>
    <name>A0A8J4Q467_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000222</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000004">
        <property type="protein sequence ID" value="KAF2078472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q467"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="534" mass="61091" checksum="F05719EF09EDB919" modified="2022-05-25" version="1">MNLFLSIYRNCYIRSLIRNNVFKDTIIDIPDIEYLNNNHQYLSLFSNDDKLKYNIYITLEINSTQDLFQFMNNSFKSLVNDIELYVGVDLLQQYPLLFSNDIIDLRRLLHDGVDRLSFDIDKVLHCAGVVMPLSVRELFIKSGETQYNDSLVTSLLLESMLCNLPTGLKVLAIPDCFQLSGSNEETRFRLPDSIVDLRYSGECACLDRFIVPPNKVFEGTVAKASSLKHLDWLRDKQWINKVYLHEAISNRTYINQTNQIPSHIKKIDVYGMVFGAENVTVLESLPHGLLEITCEKMECIDEQQPVGTLLKHHLPHLKRLAVAQWPDRITDGTFPDSLEYLLIYEYYKALDAHVLPSNLKSLNLGYLNQPLEIGSLPNSITNLSLDSFDQPLQTSVLPSQLKHLNMYRFTNTIEPRSLPRSLSTLYLNMFQGTFQHVCALDNLTKLIVHTLNQSIVNVLVNVTKIALSFTSIADNTSLSNTHITDLCLQNLDWNEILTLQDGFLPVCLVKLELTRIDIQSINTIPPSCKIFKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4B7</accession>
    <name>A0A8J4Q4B7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_000764</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000015">
        <property type="protein sequence ID" value="KAF2077964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4B7"/>
    <dbReference type="OrthoDB" id="341421at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19177">
        <property type="entry name" value="SET_SETD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1420.10">
        <property type="entry name" value="Rubisco LSMT, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015353">
        <property type="entry name" value="Rubisco_LSMT_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036464">
        <property type="entry name" value="Rubisco_LSMT_subst-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044429">
        <property type="entry name" value="SETD4_SET"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF151">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09273">
        <property type="entry name" value="Rubis-subs-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81822">
        <property type="entry name" value="RuBisCo LSMT C-terminal, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SET" evidence="4">
        <location>
            <begin position="31"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2077964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="445" mass="51020" checksum="A943B8BE71BC1145" modified="2022-05-25" version="1">MTDQSQQQQQQGDTKRCNLNIQLNNDIVWNKNLCVYDFSDTGRGVVALDDIEANEVLISVPTALLLHSRSLFSIQEFNAPSHTLQLINAKLDSKQRICLRLVVERCLKQRSKWYTYLLDVPASYQTISNYSDQDIQSLYYPPYIAEAVKLKNQLQDSFSRFTAIVDQVIATTANSNIDHDISKSDITNKESYEWAWGIIQTRTYYYSKNLGGGSNSSNSNNKDKDDCTVVPFADFFNHSCSVDTEASFNDKTGYYQVITKTPFAKDAQVFISYGKHSNFTLLSHYGFVVQDNPFEDISLIQSDAVTPNLYTPTTGDQVKTHEKKIKILETYGLGISGKFILCLDDALPVSWNYLSTLKVLFMSKQEINQEKEQNLFYYDQSISVENDRLVYTYLCNLVESLLKGFNHHYQSNRNRLKSEIQTIILSHINILTNCLHWLTKEMPNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4G8</accession>
    <name>A0A8J4Q4G8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">G-protein coupled receptors family 2 profile 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_000185</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000003">
        <property type="protein sequence ID" value="KAF2078562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4G8"/>
    <dbReference type="OrthoDB" id="100006at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112:SF13">
        <property type="entry name" value="CYCLIC AMP RECEPTOR-LIKE PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 157-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05462">
        <property type="entry name" value="Dicty_CAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="17"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="85"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="131"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="11">
        <location>
            <begin position="16"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="30"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="323"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="328"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="348"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2078562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="357" mass="41038" checksum="29E20DBC43485BF4" modified="2022-05-25" version="1">MVDNICSPGDKEKLSLAAINIIVSALSLIGSLLTIISFLWKRIRTYRVEKEREKSLYSVTNGSSSEYINGAPSSTSKQPTSKLPILIFFLSISDFFTSLFIIISQAYLIGDSESYTDSTPLRYHLNFSPCILFRALIQFFFLSTFFWTTCISYYLYHQLSSPDKENHLMYIFNGISWGIPFAIILGLGLGNHFIMDPTTGWCEVTKTYEFTLWFLPLFICLVLCTVYYIKLRRLFRSKFEYRLQINDRLKQLDSTISRRLTLYIIVFIICWLPDVVQHFISLFSTCSFFPLMILQNILAPSQGFWNFWVYSFTNKVVRIQHPFGGNGSNNNTNPSTNPTPGGGSGKYTNDETKRLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q6Y8</accession>
    <name>A0A8J4Q6Y8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003347</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000103">
        <property type="protein sequence ID" value="KAF2075371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q6Y8"/>
    <dbReference type="OrthoDB" id="287041at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="FCP1 homology" evidence="3">
        <location>
            <begin position="181"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="362" mass="41156" checksum="DF36F7B6AFEA0FEA" modified="2022-05-25" version="1">MFSSIISKNVVGKTASSFYGIRALNVSAGQKGLFLNSRFYSTQNNNNQKKENTNNKENKQDDKNKKKVEEEEDEDEDEEFKKRKSFIPSAVSNIGTGILLSVLIGSTVGYALYHYKQDLSEDEVHQLKDWEISIRDKVGRPWREFFDNMFNNLRDSSPVFESVFGTAPTPKVISPRVPGSKEYLLVIDAEAISELSTHAKHPALFRRAGLDYLLSNLYRDYEIYINYSNPSVKIESINYKLDPSQTYISGSFNFDSGVKERNIFTKNYDMFDRDPKKIIFLDTHNYDHPNVINIGKYNHKSKDATLIHLTPVLAEFSKKKMKDIPTEIASIKGTGKSLNKSLDEYLQTKKQAAAAAATPAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q8C9</accession>
    <name>A0A8J4Q8C9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Polyketide synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_002060</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pantetheine 4'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:47942"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000054">
        <property type="protein sequence ID" value="KAF2076631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00831">
        <property type="entry name" value="CHS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05195">
        <property type="entry name" value="enoyl_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00833">
        <property type="entry name" value="PKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000209">
        <property type="entry name" value="Polyketide synthase Pks12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.110">
        <property type="entry name" value="Polyketide synthase dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012328">
        <property type="entry name" value="Chalcone/stilbene_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001099">
        <property type="entry name" value="Chalcone/stilbene_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032821">
        <property type="entry name" value="PKS_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042104">
        <property type="entry name" value="PKS_dehydratase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049551">
        <property type="entry name" value="PKS_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049552">
        <property type="entry name" value="PKS_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013968">
        <property type="entry name" value="PKS_KR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049900">
        <property type="entry name" value="PKS_mFAS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050444">
        <property type="entry name" value="Polyketide_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002364">
        <property type="entry name" value="Quin_OxRdtase/zeta-crystal_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45681:SF6">
        <property type="entry name" value="POLYKETIDE SYNTHASE 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45681">
        <property type="entry name" value="POLYKETIDE SYNTHASE 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02797">
        <property type="entry name" value="Chal_sti_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00195">
        <property type="entry name" value="Chal_sti_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22621">
        <property type="entry name" value="CurL-like_PKS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16197">
        <property type="entry name" value="KAsynt_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08659">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21089">
        <property type="entry name" value="PKS_DH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14765">
        <property type="entry name" value="PS-DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="Probable ACP-binding domain of malonyl-CoA ACP transacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52019">
        <property type="entry name" value="PKS_MFAS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01162">
        <property type="entry name" value="QOR_ZETA_CRYSTAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="11">
        <location>
            <begin position="21"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="PKS/mFAS DH" evidence="12">
        <location>
            <begin position="952"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="10">
        <location>
            <begin position="2189"/>
            <end position="2267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="450"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal hotdog fold" evidence="8">
        <location>
            <begin position="952"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal hotdog fold" evidence="8">
        <location>
            <begin position="1091"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="2156"/>
            <end position="2186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="2269"/>
            <end position="2307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="2399"/>
            <end position="2425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="2156"/>
            <end position="2170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="2171"/>
            <end position="2181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="2289"/>
            <end position="2307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="2399"/>
            <end position="2423"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for dehydratase activity" evidence="8">
        <location>
            <position position="983"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for dehydratase activity" evidence="8">
        <location>
            <position position="1159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2076631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2798" mass="309296" checksum="75438A98181497B0" modified="2022-05-25" version="1">MNLKLKSSNSSVSSSSISNTKEPIAIVGIGCKFPGGVNSYEEFIKVLKNGKDCLTKIPEDRWNADTIARKSFRLNNRIGGFINDIDQFDNTFFGISPKEAQQMDPQQRLLLHITIEALQDANISLDQLKGTNTGVFIGSSSSDYSRNLDACEINQFTTPGSNTSFLSNRISYFLDVNGPSMTINTACSSSLVAIHLGATSIWSGECKYALVGGSNIISSPLQSYDYGKAGLLSTDPQGRCFAFEPRASGYVRAEGCGILVLKKLSQAIKDKDDIYSVILNTGNNSNGKTPSGITSPRSASQESLIKQLLKEVNIDTNKIGYFECHGTGTQMGDLNELMAVGKSIGMSKSEPLVIGSVKANVGHLEGASGICGVIKTIACIREQFYAPQCLFEKYNPKIPFGELNLKVQTELSPWEDEERIAGINSFGVGGSNSNVFLASFKSFNNSANSSANSSPSTLSVSSARSTPPSSPSSPQLSQMFTVPINNDLFTVSVNTNEAQDLRQRVLDILEMLNKDDGSLSFKDLCYTANVRTTHFNNRVAFMTNSLDDLKSKLQLFANDPSSFNPCIISSKKCVSSGKKKLLYVMSGQGQQWIKMGHDLFKVSTVFKNEFTKVSSLFQGISGWSIVEKLFDTNNTETIDDTWLAQPSIFAVQVALSALLKSLNVEPSAIIGHSLGELSAVYLAGILSLEDCIKLLWVRSHLQNKTTGSGKMAVVLASRESTNALIAKHKFVNRICVCGNNSPKGVGIAGDSNAIAQFSDCLAKEGVTCKPIKINAGFHSHLMDPIKEEFYKIFPNIQSKASTIPFYSTTLGKYIDAEDCKTIIGNADYWWNNIRDSVLFKEGMDEIFKNDEFDAILEISAHPIVSYFINQCMKDHAKKPSYPVYPTLNRDANGMDSILGTMMKLYCIGHTFKYEAMYPSKLYTAVKLPNRRWKLESFWSETQQRLVDRLSPPKFVSLEKRIFSVTPSFEIRLSQERFQYLKDHKIQGISIIPFSFMLEAVYAAIAELPVPQSFQVTGFEVKSAITIDSKMSNIIGINFNSDCTTFEIGSMESAIPVQKWIIHAKGSVAYNNHYVRSKDSFSPIHLLNSNNSTLKKYESKQFYSEIEKLHYNYGPAFRALEKIHIIDTTSQVSCFKLPACTDLSGHLSSDFRSIHPSLLDGVFQAAFMPGSEQDKGLWIPQQFESLSINIPSSTSAVTMDINKPMYCYSKINNYNNSNTFTTSIKMFDYNGTLFAEINNLHMRLLSKSNIPPTPTIPSIFKSETINQQIEKTDTTESISQAGVKNLFKYQWINVRSEPISSERFFPNSTTVIFSSYNSKTDPMTQHIIKESIKLGHTDKNMYIVTSDNGSFSDFVPGQGNYFGIKYTQSLSDFDHIMQVIKRNSNGEVRFIILSEFYLCQEPLPCSQSAKFDQANINYLHLTKQIINHQLVGKLYLITKEAQFLGSEHDTKVNLVDYSLVGLTRVFTNEYTDIVCSMIDITSSTTPSTLTNEMHSIQTAYNTKWEVALRAQGRYTYEFVHHDISNSAQVSKSRKDRKYQVEIGNNGVISDLQLIEVKRQSPKANQVEVNVLVSSINFRDILKALGRDYDSIHIPTIGDEFAGIVTEVGSNVSHLKVGDKVFGINMSRAMTTFVTCDSDLVFPIPEGMNYQDACTLPIVFLTSWYSLVVQGRLKKNEKVLIHSAAGGVGLASVQIAKFIGADIYVTVGSQDKHEYLVKEFNLDPNHIFNSRSISFYNDIMHATQGLGVDVVLNSLSGEYIEKSILALAPYGRFVEIGKKDIYGDSKIGLLPFKNNLSYLAVDIAQMTENRRSYLQEMMNEILPLFASSSAQLKPLKTTVFQCNEIVKSLRFMSAGSHIGKILVDWNNIDSNIIKTVDLDVQLDRNATYMITGFGGLAQTVLKEFSTTYNMKNVVVVSKNGINSEDKKQVLESLKENGINVSVQQCDLSNYEQVGQLFTTIKTTLPPLKGIIHTSCQLSDKRLIESDKSLFKTTFESKAKAAWNLHNESIAQKCSLDIFMTIGSVTSVFGNLGQSSYTMSNRFVEGLAHLRHAMGLAASCIHMAPIPEVGMSTQGHSVLFLLKNMGFTPYPSLKQMCSDLFKVFNRQEVVVLGECDIITWINSVPHFVGKSNFLNEKQWSVVVNGAEMSGDMSIDYELEEEEQDDSDSYESDNETTNTSSTNNNGGVLASNMDTAQVKTILKEIISDIMEMKKDNIDDTTSLSDYGLDSLLSSEMANAIHKRLDVFVPSLTLLDRKTNLDNLVKTVQKPITPPISSPPLRPVSQKKVKKVRPSPSSVVTKKSSPPTTTTTIVPTIAISSPISHKNSMVSPAIKSFEPLPSVVVSPAFSYAYSDASTISAPPTPNNSDFIPKTPVSIICAPTTIVTQPPIPKISFNVEEKISSPSSVNTTLPNSEFTSPPSQSTPRSNIDHMANVDNSSDPLKTVFYEIAPIAPPFCRTQNDILQENIKYTGIPEFYSTVFKNCKIKNRYFWKDPKDMDLITLARLSIEEKHQMFRNLVGDSVVKAGRTVIEKSGIDPSLISHIVGVTSTGIIAPSIDVMLIEQLGLPSKCGRTMINFMGCGAAVIAARTAMVYAKARPGTYVLVVAVEASSLNMKIDPDNRGDIVSHCIFSDGCAASLITTQPSSKVVGKMAIVDDISFLMRDSTHVLNMFIGNNGIDLTLKPELSAAILKHINGVLTEFLGSNNMSVKDIEFWAVHPGGRKILEAVHNGLDLSPEDLSESYEVMRRYGNMVGCSAFFVLKRILEKHKILQEEGDNGYKSGIFMAFSPGASIEALLLKQVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQC7</accession>
    <name>A0A8J4UQC7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_008184</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000483">
        <property type="protein sequence ID" value="KAF2070501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQC7"/>
    <dbReference type="OrthoDB" id="245173at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019335">
        <property type="entry name" value="COG7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21443">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21443:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10191">
        <property type="entry name" value="COG7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="576"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="841"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="588"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2070501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="973" mass="112094" checksum="92AEB2B41AC41243" modified="2022-05-25" version="1">MKSVNKDIDSIFSSKEFNTKAWLNSVFSSHQNDAKLSNNNNNNNNDSSLEVLLESVSSNILSNLQIHWIELNMSLENTTSESLLIVPKAIREIDRIRNESLNLKNKIKIINQKLEAINILQQQQQQQISPTTNSNNNNNNSIINQNNTIQLISKMDLVKNRMEKSIRSLQEAEKLLNFSQQVDTLFQSNDFLKISEKLEEVKQSLSVLNDVPEFRQQASKFVAYQDRLETMLKPQLIIALNAKNLDVCSQYLKIFQNIQKEEKFYEYYYTCKSDPLKTLWNSYTIKNLTQINNTAIVSPPINNNSNNNFLSPNYNSPISTSPTGTLSPTYSSTTTTTTTSTIDSGDTFQNWLSKFYDEVLITLNSEHSWLYNLTPTNYQKMLENMLIYLFNSINQTFQSRIDSAINQKTNNTNSSGIVKIQDLLSIYKTTLSFLRSLSPIFNDSLNKQEKLFKTILEPFKYFQSKFFEYELKYLKSNIQNLNIIKKNDYLSTIKNIETTIPKIFIILQSSIERFLEFTLCSDIDSYLNVLNQFFIDFISICLKDPLQELKVLAEIYSPNILNLLSSASGTLSPSTLIDKGHNRSNSNSGATTGSRTHSRSSSMNSQQSMTNLQQNWEYFQGSMRLLQSINQLLLKFQSFESNFYLNLTHYLSKESDQLITFIRKLILQDVVKLNKLQKTISLLESISQNNVKLTTNNNNSKEEKKYILQDSFKEICTFLSLAQNFVFETMIYFIKSKLKDLPKMVSTEWVEIHQQLLNQQQQSSSTHFEFLNSNNSSNTTSSLSSTNINYMTLIAEHLLTIPQQLDPYSEEESIRFSSLIAIKYPIKTLNSHDDEFYQKIVAQYHKEEIELNNSIKEQEQQEQQEEQEEIQDSIAHQWITLVAKATEKLYLQSIMEIISLSDFAAQQISNDIEYLFNVLSALGVPPDSLLAKTLEFIKLDKNTLTTIFTNNSTSFSNAEKNICNLLIKMKSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQJ5</accession>
    <name>A0A8J4UQJ5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Matrin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_007903</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000447">
        <property type="protein sequence ID" value="KAF2070771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQJ5"/>
    <dbReference type="OrthoDB" id="1925236at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000690">
        <property type="entry name" value="Matrin/U1-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041661">
        <property type="entry name" value="ZN622/Rei1/Reh1_Znf-C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12756">
        <property type="entry name" value="zf-C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50171">
        <property type="entry name" value="ZF_MATRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Matrin-type" evidence="7">
        <location>
            <begin position="115"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2070771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="187" mass="22425" checksum="1E70D24D256D648D" modified="2022-05-25" version="1">MNEEDNIQDLDTSPKSFESQQPLQTIHNNSKNTSSSSSATDDDKRWWEKLSDQEKQEIKLSGKKHLKSIQKQVCDQLKCEYCHWVKFTSLRMYRLHMLSKPHLKREQRSKISDTWSCKICNLFLPNESEWTKHSIGKKHKMKMKEYSEKHYPLVRPYFESDLKNLKQNEFKEEREEDGDDLYSSFIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQS6</accession>
    <name>A0A8J4UQS6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_007590</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000414">
        <property type="protein sequence ID" value="KAF2071086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="66620at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030587">
        <property type="term" value="P:sorocarp development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="422"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="452"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="492"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="528"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="558"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="658"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1060"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1093"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1134"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1172"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1202"/>
            <end position="1222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1300"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="74"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="721"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2071086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1328" mass="149981" checksum="F5945A8A5FB2C6EA" modified="2022-05-25" version="1">MEEIDNSNNSPSYRDDIPIPDNNTPLEEGGVELQQIKFKKVGSSDAIVDINPSNLEQHIREKLTNSPDKTGMYVTAKNLSFYVDNGKKKVSEFEKRTYLLNDLSFSLKPGRMVLLMGSPGAGKSVLIKTLADRMSAGTLEGELLFNNRPIDHETHQKDTIYVNQEDRHIALLTVKETLEFSAQCNMGENVSEQEKKERVDMILEQLGLSHTVNTIIGNQFFRGISGGQKRRVTIASEFTKCPNLILMDEPTTGLDSATAFNVCNKVKSITIEGKTSTIISLLQPSPELTNLFDDVMILSEHGKLAYFGPMESMLPYFESINISPLPNQPLAEFIQEVAEDPKKYISDGSDQVEHIDLQKLFKESIYYQGVQDLINQHSSITNLTDHSNGNNHENSMKYEFKLLLQRHLKIMKILRPQYVTRWVQALFMGFVVGSLFFQMGNTQADARNRFGLIYYAMVLHIWTTIGSIEEFYTLRSIYYDQKDGKFYKPFGYFITIVITKFPIAIIEALLFSITCYWISGFRARADSFLIFVLGMFLTNIIAQGIFQACSAFVESQLMTSLITPAIVVLCMIFSGYMLPKPQIPGWWIWTHYLSPLKYVIDMISSNELYGLTFGCSPEELIPPPSAEDFISVYQGVQICPITEGEVFLKQFGMSENFYFRWIDIVIVLAFAIALFGLFYVGLVYVKFEAKKPPKTVDQKKKKAKKDKKVVASSHHMKGCYMTIQNLNYFVDSVQKNKETGKNEKVKLHLLTNVNGYVVPGMCALMGPSGAGKSTLLDVLSRRKNMGEITGEIKVNNQSIEDINLNRFTAYVEQQDILSANLTVFEAIEFSANCRLPASYSISDKEKLINEILEVLNLNKLKNTKIGANPTIGISLANRKKVSIGIELASDPHLLFLDEPTSGLDSTAALKVMNCVKKIADTGRTVICTIHQPSQEIFEKFDQLLLLDKGNVIYFGKTGEDSKEVVEYFTNLGYQHDEKRNPADFILEIAENKTHLNPIESYKESIQCRETISKLADISSIVPEGHDIPKYKGLYSAPFGIIMRSLLKRAWLNHVRRPQTIVLRFLRSFIPALVIGTMFLRLGNDQSDARNKIAMVFLGFLFAGMASIGKIPTVIEDRSVYYRDSASGTYPPVLYLLAGFITDIPMCLLTTFSYWIPFYFLTGLAIGDHGSKFFFSLGVFFLVVMTYDALAMCFALTLPTIPIATLFNGMSLNMYGLFGGFFIPRVNIPKAWLFMHYAVGSKYGLETLSVNELEDQPFYCPNNEGAYEISLPNNSTVYYCPINNGNDMIARYGFDRDRKGWNCLILGGYIIGYTFIGYLALKYIKHMKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQW0</accession>
    <name>A0A8J4UQW0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Beta-lactamase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007438</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000397">
        <property type="protein sequence ID" value="KAF2071236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQW0"/>
    <dbReference type="OrthoDB" id="5946976at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050789">
        <property type="entry name" value="Diverse_Enzym_Activities"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43283">
        <property type="entry name" value="BETA-LACTAMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43283:SF7">
        <property type="entry name" value="BETA-LACTAMASE-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase-related" evidence="2">
        <location>
            <begin position="110"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="422" mass="47859" checksum="C318B0C5E5449C76" modified="2022-05-25" version="1">MNIPTKLNVQDPVEDQDQSDPAKKGWMVGCPPPADKLIKFIPADNKFPKMRWSLANIRQLVPTAQISRGPGPISNLPTRYHDISNVTWVDAEGKTKTFMDMMNLTYTDGLMVLHKGNVIYEKYFGVMNPTKPHLAMSVTKSFVGTLGAILVHEKLIDDTKPVIHYVPELAKTAYGDATVRQVLDMTISVKYSEDYTDPNAEVWNYSQSAGSMPRFEDYHGPQTLYEFLLTLEKDKEHQHDERFWYKTVNTEILAWIIRRVTGKPLAIYLSERIWQRMGAADDAYFMVDSIGTESAGGGYNTCLHDLARFGEVMRNRGYFNGQQVIPSEVIDDIKRGGDKAKFAPAGYETLKGWSYRNMWWVGQDAVGEFEARGIHGQRIHINSKAEMTIVRYASHPHASNIINDPPTQAAYRALAQYLENLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UR99</accession>
    <name>A0A8J4UR99_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006843</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000333">
        <property type="protein sequence ID" value="KAF2071851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UR99"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="116" mass="13234" checksum="D5F2FFED54ED3865" modified="2022-05-25" version="1">MKLKKSYLDKAKDKNITFTVNGMVMYYDQSTGSDMGAKVIQALALLKVNKYPSESETVCYNTNTIYEHCLSKYAAFASFCDRNPSSFNYVTRVLIQIYMYLLNIHSMAIAQAVQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4US28</accession>
    <name>A0A8J4US28_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_005591</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000227">
        <property type="protein sequence ID" value="KAF2073096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4US28"/>
    <dbReference type="OrthoDB" id="18777at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05379">
        <property type="entry name" value="CAP_bacterial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31157">
        <property type="entry name" value="SCP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31157:SF1">
        <property type="entry name" value="SCP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035283011" description="SCP domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="3">
        <location>
            <begin position="77"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="318"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2073096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="432" mass="45458" checksum="4D054EE90CF5D7E7" modified="2022-05-25" version="1">MKYLLSVVLLLLVSTTTTVYGYGEIDSDGNPNWFERESLTLANALRISPVAYKVHFMPTSIYSNLDAILTPSNFSSSVAPVYYNNTWNRLAKAHSYDMAVNNCFSHDDCNKTKSDTRVQYYLGGCTPNARAENIAYGTPTGIETNNLLICEASNDVCISDAGSTYSQIGHRINLMGSNYQVMGVGSYKVGSGYVYSTQNFGSKSCQGTVPNTPIHHGSHTFNVTGSSVIYIVSYYSTTAATSAKIYIDGTGFDLTRLYGTATQGFYVYKPSTYTACQNYAFEFKTATATFKFPETGSLTTATKSNACTASWTSTNVFSGTSTTSSTGSPTSTSATSSSSTSTSATSSSPTSTSATSSSPTSTSATSSSSSSSTSTPSPTPNPSSSSSSSSSSSTGTGGSSTGGPVDEPNNATKTFSNIYLVLIFSIFGLIVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UTJ7</accession>
    <name>A0A8J4UTJ7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003862</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000127">
        <property type="protein sequence ID" value="KAF2074836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1658288at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008782">
        <property type="term" value="F:adenosylhomocysteine nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008930">
        <property type="term" value="F:methylthioadenosine nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019284">
        <property type="term" value="P:L-methionine salvage from S-adenosylmethionine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46832">
        <property type="entry name" value="5'-METHYLTHIOADENOSINE/S-ADENOSYLHOMOCYSTEINE NUCLEOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46832:SF1">
        <property type="entry name" value="5'-METHYLTHIOADENOSINE_S-ADENOSYLHOMOCYSTEINE NUCLEOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NB-ARC" evidence="1">
        <location>
            <begin position="393"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1990" mass="232278" checksum="CD10CD6039A56788" modified="2022-05-25" version="1">MKESCVCLLHPDYNSVASTISIFKKYANVEFALGAHDNISFREGVLEVNSKTKVRMILSFADSDNILLFQKFKPVLAILLGSCSGTKEETSRGDVMVCRQSINYDTCRKTNDGSQWEMSKSLGPSDAFIQWLQLLDPESSQWKEYPSIKASDTYKKEWLLRAHYEYQSDKENSPWLKENGFNVTSNIPRNKCVVDMFGSIEEYRKAVKELIDQKKIILNQVPNQVVEEIMDSISIYDEYPYDVFQEIKPKLHFSHFGSGSAIEQESDVSRASVAFKKCKSISSNIKAIDRDTHSFYKACENCKKMDSISIKGVVDHANGDLDNVDSYVDYANQVTASFALYLLNNFNYQKNDLFKNINNISSLPKNIVERKNLDNRNYIDVLLESFKSNQSVPKKMISLSGLGGIGKTTLAKYFAYYCIENQMFNYIFWINSQTLSSMAQSYSTIFTDLGEKEIPSSEERTIALFKSKVQELENEETRFLLIYDNVDDFSVLNEKPIGKYIDILVTSRSRKANDQVQLVELGLLNKEESRLIFEKWNPNISKDQEKLDKSIDQLSKLLQFLPLAISQAAAYINQQGIEVQEYIDRFNDLKETELLQDDIELDKSYQRIIGTTLSIFKNTTKENKNIESRSIVLLHILALLNPDQIQLKALEIISKDLVLNIKELLYMLQSYSLINYDGYEYISIHRVIQRLLILDESNHHYLDSIHQNIANYFYHSNSNKNNNRDISITQYKLLNNFNSHLLNIYPIIKIVYKNHENSSNLELSNQLLYFQILMMLDHIQLSGINNVDKDMIHQILNESKVETPQQEKQSNNVHIDKIVDANLFYQFIKDQQELCQSVDKNIALKLYSQFLHQDNNNNNNNNNNNNNNNNNNNLEYQNRIKVILNFIFLCLNHYDSSSVTSSFHLFFQVSTRRMEKINIILNHLSIFSFKFSDILSLLKLENEKNKQFDNYLNSIVGLQDTSNIPYQALIGNTWEISSIMAIIFRLEINPNEIKDINFQYQVEGIMNLPGFKNDEHLYRKVGEATDIIKIIFQGKTQFFQIFGLEDLVGQISSSINNLDKEKFNFLICNIKTFLVDEMNNYMLSKVMESISNLIVIGYTQDSITRIVDYSHLLLQRNGFGITSEVIEIFSQLIQQFKDPEIILIINHTRSIVDQKTSRANETLKAISRLYSVGKLNNQNITSIVQHSKSIINDMEHVWSSFEIDKILETISIFKEIYNDETIKFIIDQTIRVRELKLLDGVSSILKKVDSLMVCFQSNDIVNFITNLTTLFGNDRTNNEIIKIIEEIEKLIIENYHNNQKIKELIDYSILIFNSENFDKSLKAKERKEFNFIIDQVQTYINQNMLFIQISNAFNLKPYKKEFNQNETNIIINNTKSLMHEKMQIFELEHIMDFVANLYSRDKNNLVFNSFQQLVDGKMDTLTQLMYSTSQCIEKFKQRQKVSFILSLVKLLCDDESTFHNMADLVDYIGWVFDNLNVPENQWTFIIKSVRSFIDKRVDQMANISISITNFISLFWKNLIVPTQIGFYQRICCLEIGFILQRISELKNRNTQDINFALNQTLFFINVGVKSDQIIDIINLITKSLKKNAGNILALNEFYEFDNTVFHKPVSNTITLLSNLKEKLRDEKQTLEIINHILSLFQYSENNLTNTIEYTVTLVDKYNSQDQTKLIPLLEYLLKFCDRWALDKANSEIIENITYIYSQNIPTREQKLLIKYYYLIKCTSEFMCSLFDLNAHLFIQTINPILTMGLPVYLDHQIFGVQTLALWKALLFFNSERRNYIIPTLCYDHGTQFKEETIERIKSQPLYLDESLCLTTNYTTSDSFIDDEYLKNNIIMDHSKTLLYQELKQLFTIMDLTEIFYIFRQLDLNQYLQKSDLIFKQFTLIAKKYANPAHHYIYLIYQSLSLCKDTEQVAGVCSILDLLYNAKEEYFCCNLHISNMIQRIQFYQYKIPNFLFTIENLFKCKSNQQQQWLNNIIIKSFDKEIKFNCNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UU15</accession>
    <name>A0A8J4UU15_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NADPH-dependent diflavin oxidoreductase 1</fullName>
            <ecNumber evidence="9">1.18.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">NADPH-dependent FMN and FAD-containing oxidoreductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_010202</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">NADPH-dependent reductase which is a central component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Transfers electrons from NADPH via its FAD and FMN prosthetic groups to the [2Fe-2S] cluster of the anamorsin/DRE2 homolog, another key component of the CIA machinery. In turn, this reduced cluster provides electrons for assembly of cytosolic iron-sulfur cluster proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>2 oxidized [2Fe-2S]-[protein] + NADPH = 2 reduced [2Fe-2S]-[protein] + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67716"/>
            <dbReference type="Rhea" id="RHEA-COMP:17327"/>
            <dbReference type="Rhea" id="RHEA-COMP:17328"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the NADPH-dependent diflavin oxidoreductase NDOR1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the N-terminal section; belongs to the flavodoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.18.1.-" evidence="9"/>
    <dbReference type="EMBL" id="AJWJ01000971">
        <property type="protein sequence ID" value="KAF2068473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UU15"/>
    <dbReference type="OrthoDB" id="1856718at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160246">
        <property type="term" value="F:NADPH-iron-sulfur [2Fe-2S] protein oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000034">
        <property type="entry name" value="NADPH-dependent diflavin oxidoreductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000032">
        <property type="entry name" value="NADPH-dependent diflavin oxidoreductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03178">
        <property type="entry name" value="NDOR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028879">
        <property type="entry name" value="NDOR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF10">
        <property type="entry name" value="NADPH-DEPENDENT DIFLAVIN OXIDOREDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00369">
        <property type="entry name" value="FLAVODOXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0274">FAD</keyword>
    <keyword evidence="4 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 9" id="KW-0288">FMN</keyword>
    <keyword evidence="7 9" id="KW-0521">NADP</keyword>
    <keyword evidence="8 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="10">
        <location>
            <begin position="4"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="11">
        <location>
            <begin position="195"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="57"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="368"/>
            <end position="371"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="403"/>
            <end position="406"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="507"/>
            <end position="508"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="550"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="591"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2068473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="591" mass="68243" checksum="5E2C8E66A8B958D3" modified="2022-05-25" version="1">MNKCTIIYATETGTSQEVAEKLSRDLIRYQMKPQIHDIQNYDKMKLPMERLVVFVLSTTGHGDVPDTMKPFWNFLLIKSLPSNSLMNLKFAVLGMGDSSYTTYNFAAKKLFTRLVSLGAKPLLRRGDADDQHDLGYDYEVEKWTSELIQSLLTIYPLPPNLIVDDNNLVLPNPKFKLNYSNQQEKDFNNNNFITNNYEKTIVQCNKRITDSEWEQDIRHIELKLDKLNYKSGDVAYILPTNPISYVDEFINLLQLNRNQIIESIIPLDLDLNQKPNVIVPTSVGELVYRYFDIMGSPRRYFFQLLSHFVSDENEKERLLFFSSSEGQDDLRTYNQKEKRNYIDVLKDFPSANIPLEYLFDLIPPIKPRPFSISSSSSLYPKTISITAGIHTFKAPLRRIKRTGLCSEYFSSLQEGSIVGVFIKESGAQLPPSPQTPIIMVGPGTGCAMFRSFIQERSYVQQNQGSDKLGKALFYFGCRHSKKDYIYQSEFEQYEKDGVISKLCVAFSRDQEKKNYVYHLINQDSKEIWDIINNQKGYFYISGSSGRMPKDVKASLLSIIKENLGIVDGDDDKVNQYFEQLEIEKRFITETW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UUV6</accession>
    <name>A0A8J4UUV6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Saposin B-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_009977</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000871">
        <property type="protein sequence ID" value="KAF2068698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UUV6"/>
    <dbReference type="OrthoDB" id="16524at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.225.10">
        <property type="entry name" value="Saposin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007856">
        <property type="entry name" value="SapB_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008138">
        <property type="entry name" value="SapB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011001">
        <property type="entry name" value="Saposin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008139">
        <property type="entry name" value="SaposinB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051428">
        <property type="entry name" value="Sphingo_Act-Surfact_Prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11480:SF98">
        <property type="entry name" value="SAPOSIN B-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11480">
        <property type="entry name" value="SAPOSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05184">
        <property type="entry name" value="SapB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03489">
        <property type="entry name" value="SapB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00741">
        <property type="entry name" value="SapB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47862">
        <property type="entry name" value="Saposin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50015">
        <property type="entry name" value="SAP_B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035323505" description="Saposin B-type domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="4">
        <location>
            <begin position="20"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="4">
        <location>
            <begin position="175"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2068698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="253" mass="28101" checksum="D09641269B816723" modified="2022-05-25" version="1">MKFLLALFAILAIVCVAQAKINKCEVCQIVVNKIESQLHENKTQEQIIHALEQSCKHLPKKYSKKCTVLVDTFGPILIKKVTAKDAHSALSICTKVRVCKKAHLLQSIESDFSDFSDFSDSDLVFATQEDVELVEAVEAVEAIQEVEEIEEVESGLLQKKHHFKPHNPFGKHGLKKYGCKVCTHIVGHVEKAVLSGKNVNEATNIASSSCSNFKIHPVVKLCKSVVGKVVHKMVDAIKRHENPHNVCKQVSLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UV91</accession>
    <name>A0A8J4UV91_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Phosphatidylethanolamine N-methyltransferase</fullName>
            <shortName evidence="15">PEAMT</shortName>
            <shortName evidence="15">PEMT</shortName>
            <ecNumber evidence="15">2.1.1.17</ecNumber>
            <ecNumber evidence="15">2.1.1.71</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Phospholipid methyltransferase</fullName>
            <shortName evidence="15">PLMT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_002323</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the three sequential steps of the methylation pathway for the biosynthesis of phosphatidylcholine, a critical and essential component for membrane structure. Uses S-adenosylmethionine (S-adenosyl-L-methionine, SAM or AdoMet) as the methyl group donor for the methylation of phosphatidylethanolamine (1,2-diacyl-sn-glycero-3-phosphoethanolamine, PE) to phosphatidylmonomethylethanolamine (1,2-diacyl-sn-glycero-3-phospho-N-methylethanolamine, PMME), PMME to phosphatidyldimethylethanolamine (1,2-diacyl-sn-glycero-3-phospho-N,N-dimethylethanolamine, PDME), and PDME to phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine, PC), producing S-adenosyl-L-homocysteine in each step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-N,N-dimethylethanolamine + S-adenosyl-L-methionine = a 1,2-diacyl-sn-glycero-3-phosphocholine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32739"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64572"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-N-methylethanolamine + S-adenosyl-L-methionine = a 1,2-diacyl-sn-glycero-3-phospho-N,N-dimethylethanolamine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32735"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64572"/>
            <dbReference type="ChEBI" id="CHEBI:64573"/>
            <dbReference type="EC" id="2.1.1.71"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + S-adenosyl-L-methionine = a 1,2-diacyl-sn-glycero-3-phospho-N-methylethanolamine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11164"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64573"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="EC" id="2.1.1.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 15">Phospholipid metabolism; phosphatidylcholine biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Endoplasmic reticulum membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Mitochondrion membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the class VI-like SAM-binding methyltransferase superfamily. PEMT/PEM2 methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.17" evidence="15"/>
    <dbReference type="EC" id="2.1.1.71" evidence="15"/>
    <dbReference type="EMBL" id="AJWJ01000063">
        <property type="protein sequence ID" value="KAF2076366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UV91"/>
    <dbReference type="OrthoDB" id="8300106at2759"/>
    <dbReference type="UniPathway" id="UPA00753"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000773">
        <property type="term" value="F:phosphatidyl-N-methylethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004608">
        <property type="term" value="F:phosphatidylethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006656">
        <property type="term" value="P:phosphatidylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1630:FF:000005">
        <property type="entry name" value="Phosphatidylethanolamine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03216">
        <property type="entry name" value="PLMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024960">
        <property type="entry name" value="PEMT/MFAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007318">
        <property type="entry name" value="Phopholipid_MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15458">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15458:SF5">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04191">
        <property type="entry name" value="PEMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005444">
        <property type="entry name" value="PEMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51599">
        <property type="entry name" value="SAM_PEMT_PEM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 15" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10 15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 15 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13 15" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="14 15" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Lumenal" evidence="15">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="intramembrane region" description="Helical" evidence="15">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="15">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="15">
        <location>
            <begin position="75"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="15">
        <location>
            <begin position="123"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="15">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="106"/>
            <end position="108"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="188"/>
            <end position="189"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2076366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="211" mass="24081" checksum="4B7E96E4D301EF28" modified="2022-05-25" version="1">MIIAPEYYEYLLAHVDYTETYFLAAIACIIFNPLWWNIQARAEYKHHYLTRLCGGSKYAGCYLIAFLIFTLGILRDYLFAQALLRQPTLEEFNRVEVEYLSYALYGIGGILVLSAYLRLGITGTYLGDYFGILMKERITGFPFNVMNNPMYNGSVMLFLAHSLAEKSLAGVAITFVVYIVYKIALIFEESFTTYIYETAAANALKSSKKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVT1</accession>
    <name>A0A8J4UVT1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_009683</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="9">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000776">
        <property type="protein sequence ID" value="KAF2069000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVT1"/>
    <dbReference type="OrthoDB" id="14633at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008240">
        <property type="term" value="F:tripeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04056">
        <property type="entry name" value="Peptidases_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11377">
        <property type="entry name" value="Pro-peptidase_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000047">
        <property type="entry name" value="Dipeptidyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015366">
        <property type="entry name" value="S53_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030400">
        <property type="entry name" value="Sedolisin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050819">
        <property type="entry name" value="Tripeptidyl-peptidase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218:SF31">
        <property type="entry name" value="PEPTIDASE S53 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218">
        <property type="entry name" value="PROTEASE S8 TRIPEPTIDYL PEPTIDASE I CLN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09286">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00944">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51695">
        <property type="entry name" value="SEDOLISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 9" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035277173" description="Peptidase S53 domain-containing protein" evidence="10">
        <location>
            <begin position="21"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S53" evidence="11">
        <location>
            <begin position="195"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9">
        <location>
            <position position="500"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="545"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="546"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="568"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="570"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2069000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="591" mass="64277" checksum="891F4EE1438A4AFC" modified="2022-05-25" version="1">MRQIFLLALLAICLAVPALSNVRDVSNWTKTTRANSQHVVEFRVALRQRNLDILESTFLDASNPKSVNYAKWWTIDQILDLVAPEEEKQLKVVNYLKSVGCFNVENRRDMIKASAEVEIVEKMFNVKMFNYVHNKKEQEIVRSGNDYSIPSEIKSLVHMVSGISELPHLKQGPKAQKIKLATSKNVNAVKADPGIVIPSTIQSLYGIPTGYTNNPDSSLCLAEFSDDQSFNKKDLKTFATDTATPVINVDHIVGPFSGSDPDLESTLDVQYGGAVAETASVWFWTVSGWMYDFVTDFVATSPVPYVVSMSWGWPEPLQCQSGIGNCKGETSEEYVDRVNVEFQKIGLMGVSLLAASGDQGAPGDGNPNCNEDKKPISTIFPGASPWVTSVGATMLIESSATQAVGDSAPICSKKKCATSTTEGVCTSPQALITTGGGFSDYSPMPSYQQDVVEAYLKSGAVFPKNTYFNASNRAFPDVSALGHNYYIIAGGEGQVVDGTSCSSPVFGAIVTLLNSYRLNNNKPTLGFLNPLLYQLYAENQSAYTDITTGNNYCTENCCSKVGYQATKGYDAVTGLGTPVFKEFLAYVQNLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVU7</accession>
    <name>A0A8J4UVU7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009624</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000759">
        <property type="protein sequence ID" value="KAF2069060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVU7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035284573" description="FZ domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="227" mass="24923" checksum="5000E44AA0497F6A" modified="2022-05-25" version="1">MKIILFLSILICLQQIAVAQPTCRKVSGSSKFCPNLPKKICTFSKDLKTVDANIHFSQEAFEHDRANWNPYLDCPEGNREFFTKPEFCRRSQPCETDDDCLLPCFDFCSSCLGNEKCKSEAKLGKVSTSNVKCFTPGQYVLPQASKSHLLQAYFATNSTTNGTATTTGTTKPKTTKQPPKNNFGSLDIFGDSGDFDSSSSQSDSSDANTLTFGSCLSLVLIVIVLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVX9</accession>
    <name>A0A8J4UVX9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Proliferating cell nuclear antigen</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009528</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000732">
        <property type="protein sequence ID" value="KAF2069150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVX9"/>
    <dbReference type="OrthoDB" id="10448180at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043626">
        <property type="term" value="C:PCNA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019985">
        <property type="term" value="P:translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000730">
        <property type="entry name" value="Pr_cel_nuc_antig"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352:SF10">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="21370" checksum="4F441F4EEE72A775" modified="2022-05-25" version="1">MLTKCFKSSKSTLDFIALIHKNDYLEIQFHKTKGYKSIYKVPEITMEIKEKIGAPLFEYPCEVLINSITLADICKELKTFQDKRLNISVNSNTITFEVSDGLIENRITIDKDKTNGVEIINKIDFQVEFKNSYFLKMDKTIKELSIKECLLQIGPNLPLGIIFSCFNNNNNDSSSSFQFYLAPVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWA2</accession>
    <name>A0A8J4UWA2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rab GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001344</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000031">
        <property type="protein sequence ID" value="KAF2077341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWA2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001447">
        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="207" mass="24077" checksum="BB418AADB5C294DB" modified="2022-05-25" version="1">MANNNNTQEKERVIIKFLILGGSQSGKSKTLLRYADNIFENQHIITIGVEFRIKYIDDLDPNYRLCLQMWDKYRGCERFRSTPHTFYRNASAIFVLFDVTDRESFNYVKEELKGIPHTSECIHEVYIVGNKIDLESKRVISREEAIQFAQDNAAKYYEISAKENINVHQMFDEVGKALFYKKINDPNFTNAKPTSSAPPPKSYCIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UX70</accession>
    <name>A0A8J4UX70_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Rho GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_008571</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000541">
        <property type="protein sequence ID" value="KAF2070108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UX70"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00157">
        <property type="entry name" value="Rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000088">
        <property type="entry name" value="Ras-related C3 botulinum toxin substrate 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2070108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="195" mass="21942" checksum="E68B97E4CB8BB591" modified="2022-05-25" version="1">MQSIKLVVVGDGAVGKTCLLISYTSNSFPTEYVPTVFDNYSANVMVDNKTVSLGLWDTAGQEDYDRLRPLSYPQTDVFLICFAIISQTSYTNVKSKWWPEVTHHCPNCTIILVGTKCDLREDRESLEKLKEKNQQPLSFAQGEQMAKDIKAFCYMECSALTQKGLKQVFDEAIKAVIFPDRDKASKSSKSKCNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXK6</accession>
    <name>A0A8J4UXK6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ATP citrate synthase</fullName>
            <ecNumber evidence="4">2.3.3.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_000101</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>oxaloacetate + acetyl-CoA + ADP + phosphate = citrate + ATP + CoA</text>
            <dbReference type="Rhea" id="RHEA:21160"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.3.3.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.8" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000002">
        <property type="protein sequence ID" value="KAF2078601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXK6"/>
    <dbReference type="OrthoDB" id="3261737at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042709">
        <property type="term" value="C:succinate-CoA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003878">
        <property type="term" value="F:ATP citrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006104">
        <property type="term" value="P:succinyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000004">
        <property type="entry name" value="ATP-citrate synthase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032263">
        <property type="entry name" value="Citrate-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056749">
        <property type="entry name" value="Citrate_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815:SF10">
        <property type="entry name" value="SUCCINATE--COA LIGASE [GDP-FORMING] SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815">
        <property type="entry name" value="SUCCINYL-COA SYNTHETASE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16114">
        <property type="entry name" value="Citrate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24948">
        <property type="entry name" value="Citrate_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-citrate synthase ATP-grasp" evidence="16">
        <location>
            <begin position="2"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-citrate synthase citrate-binding" evidence="15">
        <location>
            <begin position="249"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="430"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="439"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF16114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF24948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2078601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="461" mass="51160" checksum="CDD12E145DA9D479" modified="2022-05-25" version="1">MARKKLREYSTKYILRNNFPIYCNGDKLNLKMIQVSPTSPSLQDLAKVQDNQWLSTTKLVVKPDMLFGKRGKNGLVLLNASLEEADNFIKEKMNQPVEMGGKKGLVTHFIVEPFMPHDVEFYLSITAERDSNRIAFSNCGGIEIEENWDKVGTLDVPYGDSIDSINLHTLVDSSIPSREYVIDFIKNVYKLFSELSFHFVEMNPFTLTSQGKPYPLDARGEVDECASFKCASKWMVNGEDVEFPQPFGRGLYPEETYVNEIDEKTGASLKLTLLNPSGRIWAMVAGGGASVIYADTVADMGYGHELGNYGEYSGDPNEDDTYKYACTIISLATRNPDSRPRALLVGGGIANFTDVAVTFKGIIRAIKEYKDDIIKANISIFVRRGGPNYQSGLSSMRDIGHKLHIPIKVYGPDVNMTSIVAMAISHINGETPSTEQHSHSNTAHTNHSNGVHINGNGILNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXZ4</accession>
    <name>A0A8J4UXZ4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006903</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000339">
        <property type="protein sequence ID" value="KAF2071775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXZ4"/>
    <dbReference type="OrthoDB" id="19902at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040310">
        <property type="entry name" value="DDB_G0292248"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31648">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25544">
        <property type="entry name" value="Ependymin_amoebozoa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035230664" evidence="1">
        <location>
            <begin position="19"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="223" mass="24133" checksum="9A28937ED09AEF07" modified="2022-05-25" version="1">MKFLTLAFLMIMVALASAVCTNPSGFRSTFTSTSFNLGNGGSSYETGLINIDFAGQRASSVFEYYAENGKTYSGFTFSFAANQTSYLMGFDGKCYEATSTTPIPSGWPTVIQDIGTYTVGVFPVELYEVESDSADLKQSVLFDLDNCVLVSSYLANADQTNPGFGTINFLNVGVPSDSDFQLPQACIDVVGSSKPKIGFNHHKRNADIQVSKVLDHVLNAMKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UY52</accession>
    <name>A0A8J4UY52_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006589</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000311">
        <property type="protein sequence ID" value="KAF2072090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UY52"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="537" mass="60636" checksum="74EDD3E6E10035CB" modified="2022-05-25" version="1">MDDFMKLPDRSFIDEIDFQLEDPLKIIDGFIPNGVETLSMGYDFNQPITKSLIPASVKKLNLEKSFNFSGLHTDSFLHTSLEELYLHSTSRPIDPLTLPPSLKRLTIKNYNHTLTKQVMPMGLKYLHIQDISDLEPNSLPSSLLEIKTNSPKILDHLPNKLIWFYYFGREMDRLEAPKLTKLSFTTVNPTTTFFHSLSTNLVELSCSMGNHKPFDLVPGLLPLSLKKLTLNHYYGDIYKDSIPSNVEYCNLYFDSNQTKLKCLSLPQSIKVLIINFSLIKEYPVSPLSIVPSSVTDLTLISVAKDVLKKNVPLSVVKLELSYCMLPNSMSFPKCLESLTYFNPSKPIEIKSNHLPKSLTNLKLSSPTIKVDKNLLPPLVQLKKLFLPPMKLSPKLAPHSLSKLTVNILNSLPGPLAEGIVELTIISHLNEPVELTNTIPKSVRKLKLQMVNVHLSAGDLPEQLQSLDFCNCLYQNVLDYLPKSLVHLSNLPMIRSNPLFSLPSSLETLQMTKSSYDYNFIQKGNVIPLHLKLIKITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UY57</accession>
    <name>A0A8J4UY57_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">P-type Cu(+) transporter</fullName>
            <ecNumber evidence="3">7.2.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_006554</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.8" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000308">
        <property type="protein sequence ID" value="KAF2072135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UY57"/>
    <dbReference type="OrthoDB" id="432719at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043682">
        <property type="term" value="F:P-type divalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140581">
        <property type="term" value="F:P-type monovalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055070">
        <property type="term" value="P:copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd02094">
        <property type="entry name" value="P-type_ATPase_Cu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000001">
        <property type="entry name" value="ATPase copper transporting beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000002">
        <property type="entry name" value="Copper-transporting ATPase 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520">
        <property type="entry name" value="ATP7, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520:SF7">
        <property type="entry name" value="P-TYPE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00942">
        <property type="entry name" value="CUATPASEI"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="495"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="534"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="569"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="597"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="763"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="801"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1133"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1161"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="13">
        <location>
            <begin position="196"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="13">
        <location>
            <begin position="323"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2072135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1189" mass="129351" checksum="CFD7FA1DCECCEF84" modified="2022-05-25" version="1">MAIFSEKCNCNCVSCVCNDLLQNFMNKNVCRSSENQSNNNNDDGQDVKVQVQFESCCSPNLASVNTQKESKNACSCSSGTCCSLSQLFHHTFFRIMVPSLSQSNSETIRNSLLALEYTTNVVVDFVNTLVQVEVSVEKYIFKILGVFQLLKIPVAAIQSKECPQNSLMTIDDMEDTPLLNQNIQSPLFDQVELEYKTREFKIDGMVCRVGCANKIEISLLNNPKVESAHIDFDNSILTVQGDIPDRMVRKLLLRLGFKCKRVRSTQEMPFISPLHFEREEELLAIPKCDISKTNNNNTTVIEMEDLSTATMDIKVEQPPTEIDTVAIGVFGMTCASCVGIVEHGIKSVAGVVECSVNLLAERAEVTFHPQVCQVKDILESLDNLGFETKVIQVAKPGSFFIKVSDKSINQQSVIDEISTVQGVSLASVEVSQDPTDNNDGVVFKIDCDSMVTGPRAIIKLLFQKFEITAELYNPDASDAKESLLRKREIEKWRKLFLFSCVFTIPIIIIAMVLVPIKSITFFHKQIVPGFPVEALINMVLATPVQFISGFNFYKNSWAALKNFHGNMDLLVAVGSTCAYVYSLISVFMGIADSDFEAMHFFETSASLITFIILGRWLENIAKGNTSSAIVKLMNLQSKESVLVNVNTSNGGFQVTGEEIIPSNLIQFGDHLKVVPGASIPTDGVVAYGSSSIDESMLTGESIPVTKKVGDSVTGGTLNLDGSLFIVANKVGSESTLSQIISLVQQAQTSKAPIQALADTISKYFVPGILIIGVITFSIWIALAQTESIPMDWMHGASPFLFAFLTAISVIVIACPCALGLATPTAVMVGTGVGAAMGILIKGGKALETAHKATAILFDKTGTLTTGKMTVTDYKVTTDGSIDEKTFFKIVGAAETNSEHPIAKAIVKFCKQALLPSGAEKSEQPIEYQFPQVEEFQGVAGRGLKCILEAKALNIGNLSWMKENSIQVDQSIVDIAQDLETKGKTVIYVAYDNAFSGIMGISDIPREDSKKAIQKLTAMGLKCYMVTGDNRRAAKFIGQQVGISEEFIFSEVVPKEKAERVSHLQEIGDIVCFVGDGVNDSPALSQADVGISVATGTDIAIESSSIVLLKNSLTDVYRSIHLSRVVFRRIKINFTLALIYNLLAVPLAAGLFRVIFGVGLPPMAAAAAMVVSSLSVLSSSLLLKLYRPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UY60</accession>
    <name>A0A8J4UY60_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006526</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000306">
        <property type="protein sequence ID" value="KAF2072150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UY60"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="140"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="975" mass="113957" checksum="436A1C1C9E5348AF" modified="2022-05-25" version="1">MSDISLICKCCSPPRYYQSPVLLSVCDHIVCRDTVVHFGDKYVCSLCNSISDQLDTESDRVLDIVNEIERKYICKLHNQDIHEVCWTCNTLKCYLCNNEDCKVCSHDRKGILDCINVFNILDENIKKFMQDNTIKIQDNLDHLDQEMRNNKQIITRLRELKEYIKNRLENHYLQSLETEFSVLENRISSSINQLTETKQNINNFLINFNTKKSATQLYVQVLDWLKEKNSVCSYKIYSSTTFEEDDIIDQLLNSYYQPQQHSLTPTATPLTDQQQQFIITLKQCVDIKESIDTRPLLCYPIINSYKFLNLPKHPIVEPIINSQLDLFLNNNENDNNNNNIDDLFHILHKDYHVRLTRLFYLISEPKFQFNYLFFVKTLDDCRNILMKLDKKFSCSIIVGDRELIDLFNSNISKLTSVLIVYHRDFYFKYKDQIQYNYRNIKTLMITDNSDFKADHPNVNIFGEHVLEELSNEGLELLFNNLTDSIVSKDDIFYKPLMYTITKVRLKFMVEDITKDIRNKTKTPNDIIYNRLSYGIITAPPSHAFTFSVKRIFIFPVGEILWSLKVNYVESQTKQNNKWVPITIMQEPNECTDPYQLILTSEYAQIIVNNNTNGDVYNIKFDWNFRTVSKHVMGEGSMNLIPLSGTFESRSLGIQPLSLSNCFGSILYHPIHHKHLDKIEIDAIGYSFGTNNNNNNQNSVKRIAIRLVPGKRRINIINIQGMVHFIGKVKFIKDSDHTLIIRSNYSRFANNNQLIFKSKDNQPIPDRGNSSGNLCGDITLKDGRTIISFDIPAYINRKVQFYYLFFVKTLEDYRNILIKLGKNFLRSAIVGDWELIDLFNSKIIFKAQHPNINIFGEHVLKPLSNEDLELLFNNLTDSVVSKDDIFYKPLMYTITRVTLEFMIENLTKHIRNKTKTPNDIIYRMLSSAAVTISPSEIKPKNKIFKFLEGEMLWSFEVDYLESQTKQNNKWVPITIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYE1</accession>
    <name>A0A8J4UYE1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006097</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000266">
        <property type="protein sequence ID" value="KAF2072600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYE1"/>
    <dbReference type="OrthoDB" id="19260at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="281" mass="32658" checksum="EDF9341B3AA24DEA" modified="2022-05-25" version="1">MAKKQKIEKHSDSEDSGDDQCNHDHDDDENNDHGHSHAGGDHGHSHGGGKKQKGPPPSKTKTVIKFLILMMIVPAFYLIPVVLKSAGEIVSNNRVYLIYIWSVYIAILSVLVYLKRHLISLSSFVNQNLIWLIFTLGVFIVLDIPKMIQQVDLRTEVLFVWCTLTMIPNLIIFSLDDTDHKTLERLEEEKEQRKKKKEAEKKKKKDERKKAKLEEKMKQYTPMKRFLMNAGVYACLLLLVALLGWQTYRWYLRTQQNIQDRIRNPQTSPDIMNDIPFDIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZP6</accession>
    <name>A0A8J4UZP6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010542</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001182">
        <property type="protein sequence ID" value="KAF2068132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZP6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="439"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="509" mass="57632" checksum="D095A6E00927623C" modified="2022-05-25" version="1">MYGLAGSHEKGFIGIDVSDPDHWRGAWIQHSFPRFPVIINKRRGVSFVPPLKYRFSKKLKAAVPYLGWYRGYAFFNSNLLAKMNLDNKKGDINNNNNSDNDDDFSNGEKNTIDSQIDKLNNIINTYRFGEDIYFRAALYDGGTRSQHAFCVSINHGENFAGFFQYMSMLSDDFLNGMPQNLVQLFQNFEYDFDPYAKPVDDGLYIDGTKVKNLLDGVTPLPTANYLGQVGSRIFNFNLVPNDPDYKDPNQNLFYMKINTAASQKNDVWKTMALCTPGVDPEPCLNRPAAGQLEPFTVATWVKDNGDQWWHGIFTTPTFLIKNLDFGGGVRGSHKWTGNTILEHSKIGWRHRVPDEARGDTPELWNVCFSGGNLIPQRLKSIKSSVLVCFKSDALRLFLNRLVAPGHENMTAIKQSSKFKKGATIRLSILLDQAGLEKATSTGRVRREPQRYNSHSSGTRKSRPETINTPNKSPRFEKVLKELQDLTQSAIQEMEVLVPQAHYDLAINHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZR3</accession>
    <name>A0A8J4UZR3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sodium/calcium exchanger membrane region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_003948</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000131">
        <property type="protein sequence ID" value="KAF2074760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZR3"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015369">
        <property type="term" value="F:calcium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004713">
        <property type="entry name" value="CaH_exchang"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503:SF36">
        <property type="entry name" value="SODIUM_CALCIUM EXCHANGER MEMBRANE REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503">
        <property type="entry name" value="VACUOLAR CALCIUM ION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035232115" description="Sodium/calcium exchanger membrane region domain-containing protein" evidence="9">
        <location>
            <begin position="24"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="144"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="271"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="410"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="448"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="483"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="514"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="541"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="10">
        <location>
            <begin position="115"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="10">
        <location>
            <begin position="414"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="330"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="330"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="343"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2074760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="567" mass="61981" checksum="3FFE2E6D50DF1736" modified="2022-05-25" version="1">MKGIQLIVLFAFIGCLLASVAFASDSQDFAIVIIDDESNACSECTSGNNLSHFYCPDNKYCISNVTGGSNNWPTCASGWCTASTCQCQANQDIQDDIDCLNSFWPCTNNIGGMLFLMAGYGVILGFGAKFISEGSEMLMEILDPGLIGGLLLPFLSAFPDACIIVVAGALSDNPQAQLAIGIGTLAGSTIMLLTIPWSISLCLARTDIRNGESVDNQRTVRGLLKTGTTVDRDTQINAKIMVYTSVSYLIVQGVAFAYLHHPDSGKAVEKWFALVGFIVCFALMIIYSVYQVVQPKLQEKKMAEAKRQYLLKRTIHHFIHNLTKRKAFSPASPAAAANENSETDAPSETSPLISSEHARPPVDVKGIGLKWKSKALNKENINAQVDEEAAGASSNKEEDEDFGPRKRRRIAIRAVGYLVVGAFMVTIFSDPMVDVITRFGTKINVNLFFVSFILTPFCSNASELISSLIFASKRKKENSSLTFSALYGSATMNNTMCLGIFFALVFFKGLTWEYSAETVAILFVTCCVGIIGAARKTIKTIYAPIVFFLYPISLLLVYLLEHYAHWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0B2</accession>
    <name>A0A8J4V0B2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003249</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000100">
        <property type="protein sequence ID" value="KAF2075420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0B2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035149298" evidence="2">
        <location>
            <begin position="19"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="592"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="611" mass="68211" checksum="41DDC14F8CA2870B" modified="2022-05-25" version="1">MKFYLFICLLLTIFMVNNNNVVYSQTVVQMTSLAYDLVSNDLSFYLSGKDYALNKTFTGLYVNLNKDTTLTKTGLNYTAFVSGSNTLVKIPAVDFKTILGNGQLLPNFIQIFGMKNTDSFVFESPSIDFSLTSVSSFIDISNPTPMLISGTNIQKLTQSFVNSTTGVVTDNTKSCTYDATSGEVLSCSLANAKSGKVLVLLSLPNTQITISTSVVYQFALATFAGIKLDRFNISMKQLPATFSYSNMKLFVGKSQLDFNVLESNSIVNVQLNASCNLHYIILQWKTFSIRIDQHFNPIILEVLPKIANPAVLTNITGLYFEGKDRTMTVDGKNTIIVFYGPRIIQFYPPTLYLGTKNIQVSLDSYKSNVASITYGTGLIKDMTIEYETSPYNYTVHAKGTFFGSFGLNYNLFLANTTTPINYATVSSNYFDVGFALSSANTREVRSSDYFFMNTSDPSAPNGFITNVYQFWGKPEIVDTKILSFNSENTKLYAQNRDNTIDKPDRLLVSRGYQLFRTNDQGQVILTIKSNEGDVLENCWNIDPYQVWNGSVLIDQQSVVCCDIPDNFSGRQLFFTNPNYLVYVKYNNAIKTIVSLCSFIVPAIISIYLALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0V3</accession>
    <name>A0A8J4V0V3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Survival of motor neuron-related-splicing factor 30</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Survival motor neuron domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_002420</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in spliceosome assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Cajal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000066">
        <property type="protein sequence ID" value="KAF2076305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0V3"/>
    <dbReference type="OrthoDB" id="79171at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015030">
        <property type="term" value="C:Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21182">
        <property type="entry name" value="Tudor_SMN_SPF30-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010304">
        <property type="entry name" value="SMN_Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13681:SF26">
        <property type="entry name" value="SURVIVAL OF MOTOR NEURON-RELATED-SPLICING FACTOR 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13681">
        <property type="entry name" value="SURVIVAL OF MOTOR NEURON-RELATED-SPLICING FACTOR 30-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06003">
        <property type="entry name" value="SMN_Tudor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Tudor" evidence="9">
        <location>
            <begin position="114"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2076305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="301" mass="33866" checksum="3DD13BB5DA8CA7AE" modified="2022-05-25" version="1">MSTIQELENKLESDKKELQEIELLLEEDPNDEELINLKNDLVSLIKTTSDLIINTKRSLDTQLSNNSNTIDKALDSSNSSNNNNSINTTTQAAIALEPVLQYNDNSQIYSSSGRMTVGTQCEAQYSVDGVWYAAIIDSINKDGTYAVTYPEYGNSETLSIDKIRPPSRSQRLVANSTLETKKYQIAPDAIQQIPKSLKVLPEDTEQVKKQKLKKIQNIKSQNRLKEIEDESKKKKQEWRDFINKPKKSIPGTFTDKKKKSMFSTSDNINSKVGVIGSGRGMTENQSFTGLARKQNTFMPNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1N4</accession>
    <name>A0A8J4V1N4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LysM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008061</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000467">
        <property type="protein sequence ID" value="KAF2070622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1N4"/>
    <dbReference type="OrthoDB" id="20749at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045030">
        <property type="entry name" value="LYSM1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20932:SF8">
        <property type="entry name" value="LD22649P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20932">
        <property type="entry name" value="LYSM AND PUTATIVE PEPTIDOGLYCAN-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysM" evidence="2">
        <location>
            <begin position="90"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="56"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="251" mass="27365" checksum="DAF431E44D7C958A" modified="2022-05-25" version="1">MGSNNFDWDLFEANRSSNSNINNNSNSSSTNNSGNTSPYNSSYGDISLMTTFKTSNSNNNNSISQNSSNTSLVEETVSPATAASTDPRFIVHTLTSKDTLQGLSLKYNVKVNDLKRANNLWTQDTLFIKKTLLIPIESMDTGSNNSGSIGGSPLNTSSNSISYKTSSSMNTGKSYDQDHNTLFPEFEPMAKSFSSPKEKPILPKRTANNNNNNGSFHSVPMSTLSFSPVVNSLDHKTQSQFSLLDEELNPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1X7</accession>
    <name>A0A8J4V1X7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001027</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000023">
        <property type="protein sequence ID" value="KAF2077640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1X7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008455">
        <property type="entry name" value="HssA/B-related"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05710">
        <property type="entry name" value="Coiled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="67" mass="7109" checksum="2E9248E59F629ED9" modified="2022-05-25" version="1">MSIFQSIASLGSPNVVQSTLKSINSSANGAASVLGQNQIAFGALNLNPKRPANNPVFDVFAPFRRYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V219</accession>
    <name>A0A8J4V219_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VASt domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_000926</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000019">
        <property type="protein sequence ID" value="KAF2077805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V219"/>
    <dbReference type="OrthoDB" id="20021at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052438">
        <property type="entry name" value="Chromatin_remod/trans_coact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15572">
        <property type="entry name" value="GLIOMA TUMOR SUPPRESSOR CANDIDATE REGION GENE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15572:SF0">
        <property type="entry name" value="GLUTAMINE-RICH PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16016">
        <property type="entry name" value="VASt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VASt" evidence="4">
        <location>
            <begin position="250"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="14"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="459"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="39"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2077805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="489" mass="55745" checksum="21F0B7EE46CFF192" modified="2022-05-25" version="1" fragment="single">MDSLLSSTMFVNIEKSQSSPPANNSNSILQKQQQYKQQPSPNSTNFTSYEHLNNNNNYNPFLPQQQQQQQNFATMNNNNHLDNTDLYYNDKNMINHMSSNNNNNNNNANEIDKEIIPGFNMLDITTGTYIALDNEDTCCNKPNTTIRWDSTSIYQQESLHKSINNIEAIIHTNNNIQQLSPILQNSGSVGGNSNLNNNDRLVQSILSPQLQKTLVEQKEQVEKMEKDCFLIPPADIMECKDMTPQAQVNKFKTYMTETFEISCVKFYSLMIDSNFWAVVSKEMGFLEMKEGNWSISPSSCHQERVLNFKTPLNMAIKIGPSVASVTQTQKIRRTPTNGYFMETSSISRDVPYGDSFAVENFLSLEPCTTDTCTLTVSSGVRFIKTVWGFGGLIEKTVGQSTKEFFEVWINLAKKRVSENIFNINSNINNNVIQNNSSNSSTPIKKDNISPIIKSNNNIINNQQPQQQQQQQQQQQQQQQQQQQQQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V289</accession>
    <name>A0A8J4V289_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_002607</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000073">
        <property type="protein sequence ID" value="KAF2076093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2162691at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140268">
        <property type="term" value="C:endoplasmic reticulum-plasma membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120015">
        <property type="term" value="F:sterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051482">
        <property type="entry name" value="Cholesterol_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014772">
        <property type="entry name" value="Munc13_dom-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319:SF34">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51259">
        <property type="entry name" value="MHD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="750"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="domain" description="MHD2" evidence="3">
        <location>
            <begin position="1569"/>
            <end position="1688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1502"/>
            <end position="1526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1502"/>
            <end position="1525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2076093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1859" mass="212137" checksum="165B23719F098EF7" modified="2022-05-25" version="1">MKEKLLISVISGTELKKKKQWILVKLRLHNTKSKTKYVNGGEKIAFQKETFIFEVNEDVYPAGTLEIKVKTWEMLSSGEAFAFASVPLAPITAAMRESREFTNSLNQSTGSSIIVEEGVRDQDKQHAMTTRTVALVSKRGEEMKGAALMLHFELIPELDNTTMKSLPLMFRWFPELPHSEKVISECYCAKQPKSMVGMSHNGTMYITQNYVCFKSPSHLAKNKKNMISFREIRSIKKKMGSFYLPNAIQIRTAKKKYVFASFIHRSKAYRVLSNQWSLCGGSNSMNDVEEDDDDQEDELDDDDTMTYNDDQEDEEELAIINSSLNPTIEGLQTNIRPLSPLSKASLSTSGTRKARSLSIGKQQPLPVFSASTTPPVAATPPLAPVNENIKLVIEIEDYEHIGCANIFTITVGRDSIVKDLLDKVRSKIFSSSVPIQEQTQYMLMKSKTKRSKRSSQRLYPLPLDQILSNPDSFDNPMSWIPTKDFLLLHKSSSKSNNALLTTSSTFLTFGGASVKRFYVLESDKLISTYISSTQDILYLKKMVQSKEINVNFELVDENFKSFNINLPTNSLISDLYKEVSKHLLKRILPEEYNLYIPKNGERGIILSIDKPLDYYKIKPKCTIQIIKIPPFKSFMDKFKYFENSKNEKPLLFELVQTCFTNPLPSMKPDIDNPQFFLDKLQEKLVLDDTDVRLITKNLLFHSSFKDYSILIELKSRLEGLLLDSNKYYNTVSFLNNPVIYENWKTTEIHNISHLMKSVIFKEDCFVGDLHVHVIEGEGLGKGFEQCNAYVILSCRDDKRWKTTTIEDNTDPIWDESFVLKVNKPRAKLEMVIMNSKSAKEQEILGKIEINLKELEDLAPNTVKDAWFHLPTRGKIHLSLEYQYQFQEFNSYLNAVPERIETFKHFDHSNTDPLNDPNSSINNQHRLSIIHYPLYKTLLLFILDKQLASAPISPKLLAKQLKRMESDRSLNVDQQQDPDWIKPSYRLLLEQYRVRFGLGIITTSLIQLEIMIERYISELSPSLSNSAMTGVKNPDFIFELEEILTNIWTNTYEKKEVTEILITTTEIEGLCLALDKLLPLVQSTIGRYYESFPNNKPKGCLKSLVKVFETIIAIQNHLKNVDDLSYEQLLVQCVGRESKKKFDENVTAMGFLQLDIVSKKKQVAEITQMLIELISVIQEEINNTIPYETAFPDSVNITFTVIEVWGQCISLVIEDFCSWAPYHPQILVLVKHLLNYHNATKSICGDKLKPLPLKQLFITYVYKLLKEIKSNLNQLAVTSLAKDDKKTLASITGGYSSSFVVFFEACEGYCKDFDSLLWLPDSFSYVQLLEVFTSEIINFIKKLEEEIYHFISHTEIESPGKPTMFTLNIEPCIMMNNIDCSKTKLEMIANRIQKNLTNHLKSVDNNLDSTSKNCVETSFQMCLDDSYKTMNQILLSIDDFIISRMINNIIYCFESVLFTSPLNTKNSHYTASDLTSPLLAVNSVSTTPLVVGSNPLAHDQLQQLQQQQQPNLTSSTSSSGSSTPNPIQVATTTPSIVSIQASQSSSTSSLLSESGATGTLSARALLKYEKMILSQIKETISPKIEELYGKIKEPLFKPFLKKLWTLLIEEFQYLFIERPKDKLKLFHYNKVILNFDQIALFNSALKELMVLFHRGGSGIATPILEEKSLPIRMVVSLSEMDTTTLIDHYNQKRSVKQSHFNPPKDMIKAILATRFEFDKEARNFVNKANGVIEKPLVVDGIVVDEKVINVIPETEFIIDRYHCSYNGLFGILVISSRYLGFHSLLREVGVTLGHKISLPLTNIEEIKKTKVALLFNAIQIKSKENKIYTFSSFFDRDSVFNDIIHQMKVAQKKEKTNVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2E1</accession>
    <name>A0A8J4V2E1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007256</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000376">
        <property type="protein sequence ID" value="KAF2071432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2E1"/>
    <dbReference type="OrthoDB" id="2389974at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF122">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN SKYWALKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="174"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="7"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="365" mass="41745" checksum="59048CC68BDBF26C" modified="2022-05-25" version="1">MITSNTREDLERDNARLQKENWEYKKVGCSNTDHNSLYVQLETSNNENQRLQQEIHKINHNVEKLMLDNKTNVDNYNNKMDLLLNKIESLVRVNDSANIKIENLNQQNMKNVENSNKKIEMLSDKINSLEKENQSLHQLLKQQLASLESKLNEEHLGINDKVSKELDLLFLKSSIIEYSSFKIINDWIDDGKTMDFELLYKASGDDFSIPSFHLACDCKGPTITLIETTDGDVFGGYNSQSWNSDSIQYGDDKCFLFTLVNKHGIKPTKYLPNLIHQTYVAGFPFGGPMFGSGYDKESGSIDWDIVIGPCGSYQSFPRTYIDTTGKGKSTLTPTSSFTIKTIEIHKCVFILININSLLLYKRYKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2H9</accession>
    <name>A0A8J4V2H9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000329</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000006">
        <property type="protein sequence ID" value="KAF2078345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2H9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040405">
        <property type="entry name" value="DDB_G0275255-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34411">
        <property type="entry name" value="DUF6748 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035293084" evidence="1">
        <location>
            <begin position="19"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="296" mass="35053" checksum="A052DFB02A62B30D" modified="2022-05-25" version="1">MFKLSVLLFVIYIGCCVSSFTYRKDDVYTFRKDTTRGVFPFVGGYHVKVAGGGKDVKNDEYYVANYEFDHFIEQEQIDQYLFKGRIVRYLAVKYDTFEITDAYRLMPNTDQNNNNNDNTLYFITNNNLVTEYYNVNREHAVKNITEPYSKQITSLHMDWFLKQLYFNLVYGKFENDYFLVQKLYIRVDVNCGRNVGQWCPENKIPTYRYDDNQCVRNTGCVKPLPINCQKPNNVVCKQGFTKTGFPSHPNGCQIDYCFPSFLFTNQTAPSSFTPTQPTREEWARMRQQFQNNRRYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2V9</accession>
    <name>A0A8J4V2V9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009515</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000729">
        <property type="protein sequence ID" value="KAF2069164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2V9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="537" mass="61905" checksum="73C27E1BD26F06EA" modified="2022-05-25" version="1">MTSLPPNVIEKILNQYICDSLNHIERCHVATQNHLKITSQRLLNASLICKEWTNEIIPELKLSKIYLTQKCDDSIAAVNRLVERGFQFQSIVLSFDLLYTKILPREEYNPLTYYIRDCENLVFILDYPILIDYVVLLENASKVIVNNRHAAQEEIINCLENVTSLHFGPCDNLLIKHNYQLGNLKHLTIYSTTMDYQSILHLIKTNPFLEQVVIKLPAYFKDNEIGNLKLNEIFNLLTPSKYIKRLTVVSKGSKAYFESIANFLLNNDTCEYLEINLDVISNIDNSMSGVYPYSNINKVNRTLKQLEIKNPSKGIDLDLYELCPGLKDFHFHLQKGVYPDQRAFKAYLGYACSFITTPFSKLSSIYIGNGFSMEDYRIIVECMKANRTVTTFDLGLLGVPSAHDYLMEFFDDNHPTITDFGFYTGEYCTDELYNSIVNNKTVKNLRLMDPMSNTFENVADIDFIIDLLDNNTVLQHVCLYAFNLSKFTKDADLIESLENNKTLVSLQVMPNEFDDVIIKSNKIPFYLPNRPFLHLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2W9</accession>
    <name>A0A8J4V2W9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002403</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NGG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000066">
        <property type="protein sequence ID" value="KAF2076288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2W9"/>
    <dbReference type="OrthoDB" id="1232at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019340">
        <property type="entry name" value="Histone_AcTrfase_su3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13556:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13556">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10198">
        <property type="entry name" value="Ada3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="212"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="328"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="468"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="649"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="213"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="250"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="342"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="392"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="468"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="494"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="666"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2076288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="680" mass="76965" checksum="21339FDAEF5F419D" modified="2022-05-25" version="1">MVGGKRGRPPKNRPSNTVDDSGRSTQSIIDSHLSTDTHMTDEMDDSDTNSTSRHHDDYYSATNSAIDGHEGTLLSTSPNGDKDTSAAIPNSMIDNSGSSNNNNSDGWNLYLPSIDLVPKILENGYISKLSSMSTEPYQSDSEVTTSTTLNTENIDISPEDLDNACNDVFLFKRYIKDTKRLIEDQIYTLEQWKSMNYPNTTTFQVERQQYEKYKRKPRIKRRPIHTDPHSGTESELENDVLDKSMEDSENTTYTPTTTSLNSTTTSSNPSNSTATATTTTSIIDSSISANKNNSTKNKRLQKPYRNESDEDIDIVEVDDLSNRKKIHQHNNNVLNEDDNDSDSNNNSNNTSMPTSTTTTTTTTSTTNPSKNKRIHQPPPPSKNNKKKSGSKYQDDVHKKKKRDEVEAYKVHIGPGVFWNSIDSYFKPITDTDIEFITPKPDEFYNPYFAIPPLGTHYSEVWYYEDQERLNGKSSESHSSKHRNISRARSEMDGTPPPPPPPPNFDEGIFISTDEICMGDLSMRLLSALIEDNTIISTSTNTFSASSSGNIKFNQPLASSYSENNQLCIEQRIQMELASLGIYDDYKVPTSIPTLNHQLKEKDKDREDDEISYQIRLLQNQLRAQLETNNQLKLKALAECKSVISLHDSLRRKKNSQTNNERNYQKLMKRDKKKRRVSKPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V787</accession>
    <name>A0A8J4V787_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_002093</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S5A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000055">
        <property type="protein sequence ID" value="KAF2076607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V787"/>
    <dbReference type="OrthoDB" id="1731724at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01452">
        <property type="entry name" value="VWA_26S_proteasome_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000005">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027040">
        <property type="entry name" value="PSMD4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10223">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10223:SF0">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="4">
        <location>
            <begin position="5"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="219"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="276"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="219"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="238"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="277"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2076607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="343" mass="37525" checksum="7D86CF63E2DA6816" modified="2022-05-25" version="1">MTLEATIVCLDNSEWMRNGDFSPSRYEAQKDAVNLICAGKTQSNPESCVAIMSMAGKTPEVLVTLTQELSKVLASCNDVKIYGKIDFSTSMQIAQLALRHRQNKHQHPRIVAFVGSPLKESKEELCALAKRLKKNSIAIDIINFGEITENTDKLEAFINDVNNNDESHIVTVPPGPHILSDIILQSPIIAEGTGQYGSEFINADTDPDLAMALKLSLEEEKQRQERERKAREEANPGSTTAPSAPTTTSAPSISTDVQFEDDPELAEALALSMADANNSNNTNTSNNMDTSSTNNPSSTESDALRDQEFLNSTLNSLPGVDPNKIKNALEELSKKDEDKDEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7N6</accession>
    <name>A0A8J4V7N6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Integrator complex subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_004547</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Integrator subunit 7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000163">
        <property type="protein sequence ID" value="KAF2074144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7N6"/>
    <dbReference type="OrthoDB" id="19459at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032039">
        <property type="term" value="C:integrator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034472">
        <property type="term" value="P:snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033060">
        <property type="entry name" value="INTS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056517">
        <property type="entry name" value="INTS7_HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056516">
        <property type="entry name" value="INTS7_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13322">
        <property type="entry name" value="C1ORF73 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13322:SF2">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24437">
        <property type="entry name" value="INTS7_HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24436">
        <property type="entry name" value="INTS7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrator complex subunit 7 N-terminal" evidence="7">
        <location>
            <begin position="140"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="domain" description="Integrator complex subunit 7 helical bundle" evidence="8">
        <location>
            <begin position="692"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="53"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="599"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="53"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="599"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2074144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="946" mass="106405" checksum="66BA4D9FA80C910E" modified="2022-05-25" version="1">MSASTSRQTFGGAKRTPSLSGGSTASFGLSNQQLPPNSLFYLFNNTSTTALSSAGNLGSNLSTTTTSTTGTTTTATNLPASTSTPGTTTNTPAPSLLSPQSSTSTTGNNPINAAVPTASLPSSTIGGDQNKEDDNCGTILMEIDKGIRSGNIGDQVESILFFSHLIKYQPTPLVVNSVIVKLSDIFRNTNNTVKYRILKVFQECSHEIHKIANIDDVLKRIHSVILSNDPIARSLALRVLGSVPQCIVDKLYIHHSIRSCMQSHDQVEVEAVIFIIDKLCEISPLFSDSVIEKIHKTIQSVETPPITKLKYTRLFRHMHHSHSISNQSREMLVGLLSLYPSVLYVTAILDTLTSLASKHILYIDQHIKFLTTFGLSDPRVKVKVTSLKCLLTLSKISPHSQYPLTELFDILEQSPQNYWEIKYHALVLLSNLSITLFNDIKTNTKGLSLLFQYSLDYDLTLSELCIDILVNIVNESIVLSSNNDSSSNSNNDKDIDNICNQIVNSICIIIKTQFTSTNTIISKSSTLLNSIIKFIKKYPNYSKQISTTIISLLNEKTNEQNIKSLLHCMSIYIKFKKQVVYPFFNQILDYLSNLLNNNNNNNNNNNNNNNNNNNNNSSSNNNSNTKSTDIKTNITMSIFIILFRSYDENINEINHVTKSLVPLVKLILSNPKPNQLWCCYHLAQHAQRHGFHLISKIIYSSLINHVESECNYFWIKGLLSISCLENEISNNTNQSLVTKQLSNYHSSLLSIKSSSLQERSLYFQEEFIYLRERYLFNLLNLKSIILECTPIINNNNSNNNNNSNRISKQCLIKLDNFKELLRKYINLSNGVNGGKPIAIDTKQILDSLFSSCNIIIYIINHLLSILTNNDLQISENIKNNTFNSNHNVIDHNNKYPLYQLCLYIQKKLSSNESFNSNQEWMSLFEQVINSFLQVPCNFPSNFFNHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8L8</accession>
    <name>A0A8J4V8L8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CBF1-interacting co-repressor CIR N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003499</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000110">
        <property type="protein sequence ID" value="KAF2075194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8L8"/>
    <dbReference type="OrthoDB" id="20809at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034585">
        <property type="entry name" value="PAP-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35252">
        <property type="entry name" value="RETINITIS PIGMENTOSA 9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35252:SF1">
        <property type="entry name" value="RETINITIS PIGMENTOSA 9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="243"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="330"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="361" mass="44341" checksum="E4AE20D085091416" modified="2022-05-25" version="1">MSQRYDDHYRNDRYKDNSRYDDNDDRREDRSRHHHHHHPHHHHNRYHHNDDRDRDNSRRDSNRYDNRYDNRERESDYRNSNDRYHDSNNRKDEYYDRKYYDQDNKRYRDASFNDKRERERDLKRLKSLSSSYQKEQYEKERMIEKQQEEQNNKLEDSIPDLPENAQVREFLKSAPTKGLWMPLGKEVKVMQCWKCKSYGHRAADKECPFFLTGNQENEKNRLNREDPMNQYQEESSHHDHHVTNSNNDNSSTTTNDITDKKQLRRQLKLKQLQDLLARAEETYRKKKEKKKLKKEKHKEKHRYHKYKDHHDKHKHHHKHKKYKKKRKDCSSSSDESGSSDSSSSSSEGEHPTSTIKSKDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VB19</accession>
    <name>A0A8J4VB19_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Proteasome assembly chaperone 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000931</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000020">
        <property type="protein sequence ID" value="KAF2077744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VB19"/>
    <dbReference type="OrthoDB" id="16619at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032157">
        <property type="entry name" value="PAC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33559">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33559:SF1">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16093">
        <property type="entry name" value="PAC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="14877" checksum="A421B967CEA49A43" modified="2022-05-25" version="1">MESIDDIISNDTNLIIEKFVEVYENSKIYFMFYIFKNASSPLFVWVSDNANLDALNISMKVPMETLPITSELLNGASSSEYTTTLSQRLVLKFKVPTMLSYSISQDIPQLTAFIEKTLFELLSVSIKKVQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VBW3</accession>
    <name>A0A8J4VBW3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SAC3/GANP/THP3 conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000099</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000002">
        <property type="protein sequence ID" value="KAF2078599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VBW3"/>
    <dbReference type="OrthoDB" id="199574at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045107">
        <property type="entry name" value="SAC3/GANP/THP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005062">
        <property type="entry name" value="SAC3/GANP/THP3_conserved"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436">
        <property type="entry name" value="80 KDA MCM3-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436:SF4">
        <property type="entry name" value="LEUKOCYTE RECEPTOR CLUSTER MEMBER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03399">
        <property type="entry name" value="SAC3_GANP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC3/GANP/THP3 conserved" evidence="2">
        <location>
            <begin position="398"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="308"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="71"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="100"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="193"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="330"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="647" mass="74787" checksum="89008CA29AB99BCA" modified="2022-05-25" version="1">MSTFNYQYNGNQNGMYTDNSNYSNMSQTSNDGSYWNSQQQQQPQNDYYNNGALNQQQQPQQQQDYSNTAWSQYQQQQPNTQPSYSQIASQGVWNNNSNINSNINNNYSNNNNSNYNNNNASNYNNFNNNNNFNNNGTYNNMNYQQTSQTQQNYSQPTSAKGPSLIIPPPVFKKKASKKEAATPSPNIFHSHQHTQTTQTIPSSQPSPFKPQNITSITESKQKQAPQQKQDKTKKKELAAYVERAFKSASNQEELVEIQKLIKDKISDYQTKGIVNTIDWINEPLPKLEKEVWKQDIVPINSKTAGSVKKRKKEFKEPESETMKRFARFQPSNNSNSNSPTTGNYNNNSFYNKDYNYSSYNINSNSSNSNNNNNEELDWDSLTIKGTSTKLEKPYLRLTTAPDPATVRPEETLRKTLIFLKKKWMENEDYTYICEQFRSIRQDLTVQRIKNRFTVEVYETHARLALENNDLGQFNQCQTQLFQLYKSGLPSASEGEFFAYRLLYNIYQENSNDLTKTMSELTKELAALSYVQHALKVRTAIYTNNYCAYFRLVKDCPNMAAYLMDKITPRIRIQALQTMMKAYRPTVSLDQLIELGFKNEKDCRDFVDKSKLVWSNPHQKKEIDAKLSLSIVNQLEKPVIIIAAPDKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJL3</accession>
    <name>A0A8J4PJL3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">CAAX prenyl protease</fullName>
            <ecNumber evidence="14">3.4.24.84</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">CYY_009861</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Proteolytically removes the C-terminal three residues of farnesylated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 14">
            <text>Hydrolyzes the peptide bond -P2-(S-farnesyl or geranylgeranyl)C-P1'-P2'-P3'-COOH where P1' and P2' are amino acids with aliphatic side chains and P3' is any C-terminal residue.</text>
            <dbReference type="EC" id="3.4.24.84"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13 14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13 14">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the peptidase M48A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.84" evidence="14"/>
    <dbReference type="EMBL" id="AJWJ01000833">
        <property type="protein sequence ID" value="KAF2068815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJL3"/>
    <dbReference type="OrthoDB" id="360839at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07343">
        <property type="entry name" value="M48A_Zmpste24p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2010.10:FF:000002">
        <property type="entry name" value="CAAX prenyl protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027057">
        <property type="entry name" value="CAXX_Prtase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032456">
        <property type="entry name" value="Peptidase_M48_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10120">
        <property type="entry name" value="CAAX PRENYL PROTEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16491">
        <property type="entry name" value="Peptidase_M48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 14" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 13 14" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 1 N-terminal" evidence="16">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M48" evidence="15">
        <location>
            <begin position="69"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="12">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR627057-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR627057-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU366005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAF2068815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="279" mass="32146" checksum="68016B420BA10655" modified="2022-05-25" version="1" fragment="single">LFIKDKIMSFVLVLVIGSPVLAIVLYIMHWAGNLLWFYIWLFLLGFSLLAMTLYPTLIQPLFNTYSPVEGELQEAIFGLAKKVGFPATKMFIVNESKRSSHSNAYFYGFFKNKRIVLYDTLVKNFEKEEILAVLAHEMGHYKLNHTLKAFIISQFHVLFLFYSFGFFIYNTKMYSDFGFTTQPIFIGLILFQGLLKPIDSLVSFLLNCNSRKHEYEADTFAVKFGYDTPDHLVKLYIKDSGSLIVDPLYSAYHNSHPTLLERMANIKLQASIAKKSKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKB6</accession>
    <name>A0A8J4PKB6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ABC-2 type transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_010145</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000945">
        <property type="protein sequence ID" value="KAF2068527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKB6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF265">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="202"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="232"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="ABC-2 type transporter transmembrane" evidence="6">
        <location>
            <begin position="26"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2068527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="331" mass="37939" checksum="982445BCA9614CB3" modified="2022-05-25" version="1" fragment="single">MEDVATDLHLDFKNQLTNDPKYINDSTYFVVISRQNNSAFDIFIEYDVTASPSLLWDMIFHQVYEDKIKGYLSLIQTKIEKQIMKTFYSKDLDIQITYQIPPKITDLKYEPDSNLINFPHWTPSFFYIGFTIPIVIFIYQIVLENEKGMRNQLRILGLFDGAYILSWLTDGLIVSFIKSIIVLIFIYSNNSYIALSNAQPSILFVIFITYGFSLYSFGIFLSSFVSSSKSAVGLGLGLIIIGGAISISFGFMGTYIFHFYSYQLIPFGILISLVPFLNVSYLLYHITIFTLPTIMEGPEFYDSVEIDRHQALWISKGGKYSWSNFMQGSNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL38</accession>
    <name>A0A8J4PL38_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Endoplasmic reticulum-based factor for assembly of V-ATPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_008754</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000572">
        <property type="protein sequence ID" value="KAF2069925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL38"/>
    <dbReference type="OrthoDB" id="19981at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021013">
        <property type="entry name" value="ATPase_Vma12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31394">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31394:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11712">
        <property type="entry name" value="Vma12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="240"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="255"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2069925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="265" mass="29938" checksum="529611ED867E2342" modified="2022-05-25" version="1">MSETNTTTTKSYLDFTFTDTIKNAINSIAHITNNSSNNKKEKELIERIKTLKTKYTTNKTQFISYKDIKEISNISKEINKEISNNTTTVFYIQDLLKGSVIYIPPKPTPKIDPELEAKRQQYEKLAKDQEYARLVKNIVPKKDERNELNSISSQVSIGVNILITFFTLLAGGMYLGNRMFSSMLMGLAFGLGAGIFGVAVEVWLFVIRTSGTQLQKDKEQRDIDRAREIDVKRREFRIKKSHGLLPASPASKGKGGGDKEMVVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL45</accession>
    <name>A0A8J4PL45_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010120</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000932">
        <property type="protein sequence ID" value="KAF2068554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL45"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="790" mass="92860" checksum="33995D9081E8E266" modified="2022-05-25" version="1">MIIDSYRSVFGNVYLATKIYNSIHQIQIDRYPLKYDDIIDVGWMLKYGHVGLAREKIKRNEYLYVKPKHLFSIVANTDTQMFISLFEKNKYYALGYYDTAFESMFQNLVNVDVVKYLFQNGYARDLSNLDHLRLLDIKVLSWLLENQWLKPTPLFLISSKRDGLTYIFTSTRDTDYTKEFVDLVAQYTPSPIQHAEIEVIMRYTLNYSKGEPCPFIFDSLSLLFVKDPDIRFQIERGKGPYLNYQQACQEIKQRREKREISEIKNSNTITSCSELLLAMKDPAFESDMAVVFKIWISLLSQGEQVFYDMWNALASRLIITRHNIYFYQSRDITDDDDEDDLIQNKNTIDISKLILDAICKIGSLKVVKFVTDQGHSPKISYSPSASRYLFFKQIMDKTLSLEECHIIDKLCNASGQDDKYKFQHIHSKVLQRNQMLSKCCRHGNAANFESLYLLFQKSKLFDHLVPGLYEICMESEQYHMIPVLERHGLFLLDHHELSDLCSEYPFTRLLNSVDRMIAATTEKEVLLRFYTRLLISAIKHNDLIAVKHIFTKHKFESLSDDLKREFSSLQNIAILDYINKNRWHCFTKETLINNYVHSCFIEIPCIIRSPSPINIPLVEYLIKEKCINFEEIKSKHYYLEPFFGDDINCILEEKICFEGFVYLIDHNNYFSIDPFMNLLTNNSFDYKYFEYIYRNQHKFQTKPSFQSIFDHIVINNLNLENIQKSKDKLEALVQRYGCVLNDSHYHDLVKHTDGLTLFGIVPDSIFVSNEYHPPPIQGSTTGRPLKKQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLE6</accession>
    <name>A0A8J4PLE6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CAP-Gly domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_009660</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TBCE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000769">
        <property type="protein sequence ID" value="KAF2069020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLE6"/>
    <dbReference type="OrthoDB" id="5273213at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.190:FF:000016">
        <property type="entry name" value="Tubulin-folding cofactor E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45617">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CAP-Gly" evidence="9">
        <location>
            <begin position="56"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2069020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="509" mass="58357" checksum="B03A00A86F09E6A8" modified="2022-05-25" version="1">MSDIRNQNSISNSSSNNNSNNNDDVQRVTTTDEFKISLGERIGSDDGYIGTIRYEGKVDGFEGNWFGIEWDHPTRGKHRGTVKGKQYFQCQYPDSSGSFMKKEKIIKGVDLIHALITKFTRKLENYDDLTVHSTKSNKMIQIEMVGMDQLRERQKNLKSHSSINAYNLSINKINTNILNQCLSNLIELNLSHNYLNNWNEIIDLIIQLPLLNKLILSENRLVFNHVEIDNQIKGKGKEIQESNLTSLTLFDMGGSVTWDHVCYLTENIFKNIQVLAFGKNKLHSIQMNSSSSPSFKHLKSLDLAYNNITCLNDVKALGDLLSLEELNLNHNQITSLQLDNQCTNDSDEKYKIFCNLKRIYLSNNLINDWSEIDVLDRFKSLVELSFRDNPVLDSLILSSTTTTTTTTSTTTTTTATKTIEEEENSFNEEFNVNSISTKSTNYKDIVSLKRLHIIPRISQLLKLNLTEITSTERRDAERFFLYEYYSNNNNNKTISKTKLTYLTSIHGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMR6</accession>
    <name>A0A8J4PMR6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tetratricopeptide-like helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008931</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000600">
        <property type="protein sequence ID" value="KAF2069755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMR6"/>
    <dbReference type="OrthoDB" id="2154985at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019412">
        <property type="entry name" value="IML2/TPR_39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31859">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 39 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31859:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 39C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10300">
        <property type="entry name" value="Iml2-TPR_39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="576"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="585"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="640"/>
            <end position="649"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="669" mass="76147" checksum="95E5DB3354E2775E" modified="2022-05-25" version="1">MGIIDKDIVEAIDYVWNNNFDKADLILSSKAAYIPRYSLHYAETAFLKSFITANESDTEIALKRLKDTRELSENCIKLLESHKIPTNYPSSITTKQDFKNYLLDSKIVLGDSLYMLAVLQVVRDQKFKGIFNLRKSWKIFEDCLKQVKHDESNQIQYSPDLLECLHFGAGFFFFAISIIPQKFLKFVELVGFKADRDQGLAYIRDISNKNGIRAPFGTMVILFNNLLLPRGLYNPASQLKEAELIIEKNLERYPQGSLFQVMASHCYRKQCRIDLGVECMERAIANCSNLAKAPLIYKYELAACYCVKTEWNKAIPIFETLIQEENFQIRALCALQLAAAYIINNDKKKGLDLLGKMKLYAKKSSTVDTVIVRQAQRYLASGGRFAAFEIMYIRRDMAKMEKNMAKIVLDMLNEIAKSVGVYEKLPANAVASNAANQASAFFAKTFSFGKKKEETNAPAQVIVSESLINDRAAYLILKGAILKGIEKFDESMECFQEIIAMQSQITEKFYIPYTYYEMSESYYNKKETSKAMDLIIKCNSISNYDWEDPLKVRLRVTLDQLKRDGNAKGLIEMMEEEEDSHVDMDPSLSDDSLPSSQPTNRSSSPVFEKHNIAKQPNADGATSSDDQEFTEYITKHLSKKSSISSPSIIDDQESKNNNTKPRPFEKSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMT9</accession>
    <name>A0A8J4PMT9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008547</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000536">
        <property type="protein sequence ID" value="KAF2070134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMT9"/>
    <dbReference type="OrthoDB" id="5371818at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06127">
        <property type="entry name" value="DEDDh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006054">
        <property type="entry name" value="DnaQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00573">
        <property type="entry name" value="dnaq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30231">
        <property type="entry name" value="DNA POLYMERASE III SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30231:SF39">
        <property type="entry name" value="DNA POLYMERASE III, EPSILON SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="2">
        <location>
            <begin position="286"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="467" mass="52965" checksum="75330267502C7D9E" modified="2022-05-25" version="1">MDGSDDDDLLDFASGQMFNKSNNNVDMSSNITNSSSSSSSTVDSTIFKTPTIITNNSFISPNLAQTIDPSPLVSDTKNNTTTSGSIPVNDDLYNSFHEDNDATDAEDNKTQDDALFIPLQYTFKNNNQNNTQQDDDIDIPIQYIPKDKKLKSNNNDTSSKKSNSNNNDKSYFDYMMDKDNSDTELVNNNNNNNNNNNNNNNNNNNNNGSKLDNKDDSNRRLPNWMKRKQPEGQDIFITTKENFEMKKNVKQTLLEQNKDDNDESNNEWVLSLNAFKEIDEPILKGRVICFDLETSGFGVDDSIIEIGAVELIDGRRTGVIFQSYAQPKSNIHPKAEEIHQLSNYLLQYSPPIEMVLASFMDWVGDSPLVAHNLAFDKRMLIQELIRSKLPFDPQHICFCTMKYFRKIYVGCNYSLDSVSQKLQINKLLLRKTHGALVDSEILAIIYSHLVSLPTNHSALKIMENMKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNQ2</accession>
    <name>A0A8J4PNQ2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007871</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000444">
        <property type="protein sequence ID" value="KAF2070803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNQ2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051510">
        <property type="entry name" value="SKI8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44090:SF1">
        <property type="entry name" value="SUPERKILLER COMPLEX PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44090">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="459" mass="52542" checksum="9D004D10ECAD043E" modified="2022-05-25" version="1">MALPFLSAEILVYIYREFEARDCYNFLFICKFLTIKLEKDENFWLYLIHLHGKGPSTLIRHNLDIMELRQLNSQNKDPDASLKYIKDYFKKRSWLFKYLNNNKQHGGSSGERFKKYFKEENIILGSICSCPFESNVVIGGYDQKSSSPVLKMLNYKGETIADLLSPKIREKKNISGDVRSISCKNNIIAFNTSYSNDVGIFGIGKDRKVRENFIKVGNQRTNLQVRLNNNGDELAVCSLDGGCSLYDIEKKERLSGFSEPQMPINGFNFSFDDNLFFIAGTSKQNGRQSYKNTGYVKMWDIRQGNGQGNNSVVKCWKEYAECCSISQSEDANFLAYVMNTGITRVIDLRTDQLFSTFAPAYSRIHNFEEQNKVVNEDVCWSPGGHRMLASTSRGNIYFWNTHNGEIILDLEIKKHHESHQIDISDDGSTLYSSLGYIYSVNDVTDIAIYAYLNGLEAEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP41</accession>
    <name>A0A8J4PP41_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA primase large subunit C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_007114</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic-type primase large subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000362">
        <property type="protein sequence ID" value="KAF2071562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP41"/>
    <dbReference type="OrthoDB" id="421393at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07322">
        <property type="entry name" value="PriL_PriS_Eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058560">
        <property type="entry name" value="DNA_primase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016558">
        <property type="entry name" value="DNA_primase_lsu_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007238">
        <property type="entry name" value="DNA_primase_lsu_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10537">
        <property type="entry name" value="DNA PRIMASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10537:SF3">
        <property type="entry name" value="DNA PRIMASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04104">
        <property type="entry name" value="DNA_primase_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26466">
        <property type="entry name" value="DNA_primase_lrg_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0639">Primosome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA primase large subunit C-terminal" evidence="10">
        <location>
            <begin position="277"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2071562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="472" mass="54902" checksum="DFE80411F030B6E6" modified="2022-05-25" version="1">MLRTTNKKTKIEEEEIKGYNSSLYSIVYANVPTFECNLTQIEHTCLDRITLLDQIVKLKDSNPKGNSTSLDVQFFGLVTQYLGKHEHPSTKLKDELSHFMCRIAMCDSEEDKDWFINAEALLLNFRLSTNDLTTHLEQFTEFGWSKLTTQEFETYQKDLEYLVLTSNGKPIDLDINSFYKIPFEDVPKLVEHGKVVLKKGWAIVFKAYFRELIICTYRNYLEFAIEKVKQDKARFKTQYPEIAEILRGITRPTIVNSKLYGANSSHIKKEQIPELSKTFPLCMRVMHDHLISYGKLMHDGRLQYGTFLKGIGLSLDEALGFWKNAFSKRVTSSEFDKEYAYQFRHTYGQEGKGISYDPYSCEKIQRTFPSGKEQVHGCPYLWDSERLEKKLLELGIDPSKTYDIVEASKTQSRMACTKHFHFLHPNNNNLTGVINHPNFFFEQSKIYFKQIDEKNKKSKENSKNNNGDVVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP81</accession>
    <name>A0A8J4PP81_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Isocitrate dehydrogenase [NADP]</fullName>
            <ecNumber evidence="10">1.1.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_007019</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19629"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="10 13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="10 13">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.42" evidence="10"/>
    <dbReference type="EMBL" id="AJWJ01000352">
        <property type="protein sequence ID" value="KAF2071665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP81"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004450">
        <property type="term" value="F:isocitrate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006739">
        <property type="term" value="P:NADP+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000002">
        <property type="entry name" value="Isocitrate dehydrogenase [NADP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004790">
        <property type="entry name" value="Isocitrate_DH_NADP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00127">
        <property type="entry name" value="nadp_idh_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006156">
        <property type="entry name" value="PRK08299.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11822:SF15">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NADP] CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11822">
        <property type="entry name" value="NADP-SPECIFIC ISOCITRATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000108">
        <property type="entry name" value="IDH_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="6 10 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 10 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10 14" id="KW-0521">NADP</keyword>
    <keyword evidence="8 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="15">
        <location>
            <begin position="9"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="75"/>
            <end position="77"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="94"/>
            <end position="100"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="309"/>
            <end position="314"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="11">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="11">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2071665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="415" mass="46448" checksum="784F83B3251544FC" modified="2022-05-25" version="1">MTKIDVANPVVEMDGDEMTRVIWAMIKDKLIHPFLNLKIEYFDLGMEHRDATNDQVTIDCANAMLKYNVGIKCATITPDEQRVKEFNLKQMWKSPNGTIRNILGGTVFREPIICTNVPRLVTGWKKSIVIGRHAHGDQYKATDFVVNAPGKLEMTFTPADGSAPQKYTVFDFPGGGVAMGMYNTDESIVGFAKSCLEFALAKKWPLYLSTKNTILKKYDGRFKDIFQEVYEKEYKQQYEAAGIWYEHRLIDDMVAYAMKSEGGFVWACKNYDGDVQSDSVAQGYGSLGLMTSVLLCPDGKTVEAEAAHGTVTRHFREHQKGRETSTNPIASIFAWTRGLAHRAKLDNNAKLTAFCDALEAACIDTVEAGFMTKDLALCVKGSLENVKRSDYLNTVEYIDKVAEALKKTLNIDSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPI4</accession>
    <name>A0A8J4PPI4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">dihydrolipoyllysine-residue succinyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">2-oxoglutarate dehydrogenase complex component E2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_007216</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.61" evidence="5"/>
    <dbReference type="EMBL" id="AJWJ01000372">
        <property type="protein sequence ID" value="KAF2071463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPI4"/>
    <dbReference type="OrthoDB" id="5391403at2759"/>
    <dbReference type="UniPathway" id="UPA00868">
        <property type="reaction ID" value="UER00840"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004149">
        <property type="term" value="F:dihydrolipoyllysine-residue succinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033512">
        <property type="term" value="P:L-lysine catabolic process to acetyl-CoA via saccharopine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000006">
        <property type="entry name" value="Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050537">
        <property type="entry name" value="2-oxoacid_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006255">
        <property type="entry name" value="SucB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01347">
        <property type="entry name" value="sucB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43416:SF5">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43416">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="14">
        <location>
            <begin position="80"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="157"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="158"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="193"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2071463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="442" mass="47794" checksum="3319C9133FCA8871" modified="2022-05-25" version="1">MKTANLLKQIKFTPKSISTSSSGLRSFSVAGNRLYTTSSVQSNNTTTTTRSTLQQRQQYSSLFNNVNTTYTQRFYSAAADVVVKVPSMGDSISEGTVVSWNKKVGDSVRVDDVICSIETDKVTIDINATANGTIVELFAQEGDTVLVGNPLYKMSEGAAPPKAEAPKAQAPPKAEAPKTAEAPKAATPPPAPKAEAPKAAAPSTPAAASGSGEKRVKMTRIRQRTAQRLKDSQNTAAMLTTFNEVDMSALMNLRNKYKDEFEKKHSVKFGFMSAFVKASTIALKEQPIVNASVDEADIVYHDNINISVAVAAPKGLLVPVIRNCQNMSFADVEKELGRLSGLARNDQLAIEDSMGGTFTISNGGVYGSMFGTPIINPPQSAILGMHAIKDRPYVVNGQIVIRPIMYLALTYDHRIIDGREAVTFLKKIKDTLENPERILLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPS5</accession>
    <name>A0A8J4PPS5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Autophagy-related protein 27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">CYY_006839</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Preautophagosomal structure membrane</location>
            <topology evidence="4">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ATG27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000333">
        <property type="protein sequence ID" value="KAF2071847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPS5"/>
    <dbReference type="OrthoDB" id="29460at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018939">
        <property type="entry name" value="Autophagy-rel_prot_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15071">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15071:SF28">
        <property type="entry name" value="MRH DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09451">
        <property type="entry name" value="ATG27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0072">Autophagy</keyword>
    <keyword evidence="17" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 19" id="KW-0732">Signal</keyword>
    <keyword evidence="8 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035165409" description="Autophagy-related protein 27" evidence="19">
        <location>
            <begin position="20"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="20">
        <location>
            <begin position="20"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAF2071847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="249" mass="26836" checksum="3262DD84F68D94F5" modified="2022-05-25" version="1">MKFIAILITVLAVITIVNSQACSYTNTKNDLINLSNLTSYDTNAYNVTSTKTKNIFYYNICGAVRYCADNAQSKGTYSACGYMSKVDKVYAFGVPNMINITSLGDGNEYGATVVYNSSSACANLKRSTVLKLYCYEGTTVESLSVDDSNNCGVVIEANIPCPGVKIPVKKSGLSGGWIFIIILFSSFAAYAIFGSIINWKVRHQSGSSIFPNSSFWSNFGSLIKDGVFFIKGKVSGTPYVGGGATYTEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPT6</accession>
    <name>A0A8J4PPT6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S26</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_006988</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000349">
        <property type="protein sequence ID" value="KAF2071688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPT6"/>
    <dbReference type="OrthoDB" id="10262653at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1740.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1740.20">
        <property type="entry name" value="Ribosomal protein S26e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000892">
        <property type="entry name" value="Ribosomal_eS26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047864">
        <property type="entry name" value="Ribosomal_eS26_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038551">
        <property type="entry name" value="Ribosomal_eS26_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12538">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12538:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01283">
        <property type="entry name" value="Ribosomal_S26e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00733">
        <property type="entry name" value="RIBOSOMAL_S26E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2071688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="115" mass="13293" checksum="CA2B545FF3658890" modified="2022-05-25" version="1">MTQKRRNHGRSKHGRGSVPYIRCTNCARCVPKDKAVKRFYIRPIVENAAVKDISDQGVFNEAGYKFPRTYVKSQYCISCAIHSHIVRVRSREDRKIRTRPQRPGAKIVKAGEKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ08</accession>
    <name>A0A8J4PQ08_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">IPT/TIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_007927</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000450">
        <property type="protein sequence ID" value="KAF2070746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ08"/>
    <dbReference type="OrthoDB" id="23947at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052014">
        <property type="entry name" value="Dictyostelium_Tiger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31341">
        <property type="entry name" value="IPT/TIG DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31341:SF12">
        <property type="entry name" value="IPT_TIG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="381" mass="41847" checksum="67AB43F978C3CD3C" modified="2022-05-25" version="1" fragment="single">MRLQGNLKLFIIWVILVNVNLVICEYSLVNYNGTILAFQPGIKSISLNNNFGKPIGVNNGGCFSSFDLSPLAKSWEPSSVFHVIDCNDESGSLEYSVSSISKYNKPSTSGGPTHFTANFMKLSLTQSTIKSISTTKGPIDYTEYFDPNDSSNLTIVFPPGIGPISLIYYSSPKIYKLNFSYASPIINSVSYKDGKVSLIGHNFFNSLDLLNITMDGSDIKRLIISIDHDLIVFKKDIFIAEKFVINLSIGGTSLESPYNVVEFLPKINYLTSVPNRGGPITITGAWFFMAHQNIPVTVDINMGTIPCKNPRSPDPSGTVLICDMPPSNGSEGRLVPSILINSVANLKTDLYFEYALPSVTHYTQQGDKFSFQGENFGTQAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ12</accession>
    <name>A0A8J4PQ12_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">tRNA nucleotidyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_007131</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000363">
        <property type="protein sequence ID" value="KAF2071559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ12"/>
    <dbReference type="OrthoDB" id="445712at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052929">
        <property type="term" value="F:ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052927">
        <property type="term" value="F:CC tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001680">
        <property type="term" value="P:tRNA 3'-terminal CCA addition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05398">
        <property type="entry name" value="NT_ClassII-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3090.10">
        <property type="entry name" value="cca-adding enzyme, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002646">
        <property type="entry name" value="PolA_pol_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032828">
        <property type="entry name" value="PolyA_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13734:SF5">
        <property type="entry name" value="CCA TRNA NUCLEOTIDYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13734">
        <property type="entry name" value="TRNA-NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01743">
        <property type="entry name" value="PolyA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12627">
        <property type="entry name" value="PolyA_pol_RNAbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81891">
        <property type="entry name" value="Poly A polymerase C-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly A polymerase head" evidence="6">
        <location>
            <begin position="123"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA nucleotidyltransferase/poly(A) polymerase RNA and SrmB- binding" evidence="7">
        <location>
            <begin position="284"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2071559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="571" mass="65643" checksum="3A403248DF6AD3B9" modified="2022-05-25" version="1">MYKKLLSGSTLINIRKQCKGDIVYQQLSRLYTTSTLDLDNNNNNSKRSTVDHINKDSISHTKSNNTHINSTLLNSNNSNSIYKSNSSKIKKDSKIYLSKAEKEIIDFVCNVPKLINRDDIVIRIAGGWVRDKLINKNESTIYDLDFALENISGEEFVKLLSQYIGGRTFITKMNPSKSKHLETACLEIDGFSLEFNALRTDQYTQHTRIPVIIRGTPKEDAMRRDITINCLFYNLNTKEIEDQTGMGIDDLEQGIIRTPLCPEATLNDDPLRALRAIRFSSRLNFKIEPTLYKVIQSNEIKELVSKKVSKERILIEYTKMMSDTRFSLQYYKYLVETGLIQVIFNNSSLHYSDSIEFFKNGTTVLKPSKRIESLEYFTTLIFMNDFVNGTIDINGVSKLLKEFKSSNKTSREIQSHLLTIRSIVKVINTFIIKQQQHNFSLDQFLIDEFNSITFGLLESLKGDDLWETSIHLSRVYLTTTSKGQQQQQQDNLILSGKFWNSFTLLLKDKYQPISKSAALATFGDISKAKLPVKEIGDIMKQLFHWQWRSLCQGTQPTQSDALVYLKTIFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQ36</accession>
    <name>A0A8J4PQ36_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein pelota homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_008635</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the Pelota-HBS1L complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway. In the Pelota-HBS1L complex, pelo recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the eukaryotic release factor 1 family. Pelota subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000552">
        <property type="protein sequence ID" value="KAF2070039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQ36"/>
    <dbReference type="OrthoDB" id="10249111at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990533">
        <property type="term" value="C:Dom34-Hbs1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070966">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, no-go decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071025">
        <property type="term" value="P:RNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.870:FF:000004">
        <property type="entry name" value="Protein DOM34 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.60:FF:000004">
        <property type="entry name" value="Protein DOM34 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000008">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.870">
        <property type="entry name" value="Pelota, domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038069">
        <property type="entry name" value="Pelota/DOM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058547">
        <property type="entry name" value="Pelota_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004405">
        <property type="entry name" value="TF_pelota"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00111">
        <property type="entry name" value="pelota"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853">
        <property type="entry name" value="PELOTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853:SF0">
        <property type="entry name" value="PROTEIN PELOTA HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26356">
        <property type="entry name" value="Pelota_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159065">
        <property type="entry name" value="Dom34/Pelota N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0469">Meiosis</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="12">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="376"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="376"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2070039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="406" mass="46653" checksum="5251B5BD26D732A9" modified="2022-05-25" version="1">MKLVKKFIDKDMTGYVKLQLEEEEDLWHCYNLIAVGDELTSSTVRKIQKESATGSVTSERQKLKITIQVTKIDHDGQSSLLRVSGNVCQENRFVKLGSFHTIDLEMNREFQLYKTEWDTISLELVRTATDIGQRADVAALIMNEGLANLCLITSSMTVVKSRIEVPVPRKGRSTSDNHQKGLENFFNVILQSMHRNINFQVVKCFIIASPAFVKDKFFQYMMDQSSKNELYKEFKANKSKFILTHSSSGHRYSLKEVLSDNTIISQLSNTKAASEVKILNDFYDMLKKDPNRAFYGYDHVKKANDKLAIETLLVTDELFRGKDTQTRKKYVDLVQSVKDNKGEVKLFSGLHVTGEQLSKLTGVAAILRYPLELEDEEEEFNHEEEDEEQENNNNQFNDYDDFTSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQD7</accession>
    <name>A0A8J4PQD7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007796</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000435">
        <property type="protein sequence ID" value="KAF2070895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQD7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052258">
        <property type="entry name" value="Diverse_Func_Domain-Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37612">
        <property type="entry name" value="FIBROIN HEAVY CHAIN FIB-H LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37612:SF20">
        <property type="entry name" value="PER-HEXAMER REPEAT PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035272806" evidence="2">
        <location>
            <begin position="20"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="186" mass="17806" checksum="30BE040FB8911E8E" modified="2022-05-25" version="1">MNKLFVIAAILAVVCFVSASAYAPKNSCTCFYHDGTTKTIQAAGYDSCLEKCNVLGNLETPTCDSQCTCQCPHAVRKCNNPQPDTHSAGGHSAGGSAGGAGAGGAGSAGSSSDSTSGSSGSGSCSGSGACTGSGSGSGSGSGSGSGSGSGSGSGSGMAANLKQQEIRDATCTKCPCVKQTVSVKFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQW9</accession>
    <name>A0A8J4PQW9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006742</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000324">
        <property type="protein sequence ID" value="KAF2071948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQW9"/>
    <dbReference type="OrthoDB" id="439743at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1100" mass="126337" checksum="754551A5DBA9F3A8" modified="2022-05-25" version="1">MTTINDNDLVWISPKLYDDLNNVLRYQITIDKLLFSSSSPLLTVPHELSTLRIIISYHDITDTRDMQSCATTLPTTITHIELYFDIPYDVTNDLSSIILKLNKFLYNTSHSLRSLTISNSNSNKSSYNRDDRVESILLARDKSMVYLHIPPSIIHLEIDCLSQDIASSLCLSSVKSLKAGHCTQSSIEYFMTYLEPARILTIDVVVSDNTVKSLVIEKGTLDVKVDDRYHIHSFDITPMYNSNTRYLLFEDIDTPEEPLPENLKSLHLLGNYSSFDSIPDTVEYLICERLTSLYSIKLPRQLRYLSLGIEFDHLLTGIPLSTEYLELFLSNRWPLSYGYIPNTVRYLKINCDQQQQTDISVINIPSTIKRVYIDPFKSHLVKIIDESQYYSRPQHPLKGLVLSDSFNQILTTTSLPENLLELTFGSNYRETIIDRVLPPSLTTLVSKGATCIFATIPPNLVSACLGKHHDSLAKMSLLNEYHINYKNQNVDLESMPNLKRLVIKDCYYESPIKVPPNVKELVVKTYRFQLAPNSSLEKLYIETKMRQEPIQFPQSLKSITNISTKIELESLPFHLQELTLNYYLNRNLINSSNLKKSIIDINNNNKHSYNNNQRDKDKDLFFNIWRSVYLQFKILDFLICPFDKKETNILLVPFKPNQSTLFIKNIKDFDHNIETGIKKVIIDYDCLLALTRPIPQNFLKLMDIKYHVYQQDVSIPVNTTHILWPLNQVISPGVLPNGILSCLFGNDYNQIIPQGSLPESLVNIVFGSKFNQDLDNIHFPLKLKYLTFGNGFRQTLKSNRIPMTLKHLKLGYMTRRILKKINQFDTNIDHLEFKTGGPEQVDQTHLPKSIKYVRLNMSFQSEPCTITFLPNIKCVYTKEPQEILYVQEDNSKTIKKKMENNGKINRSIQLNLLMNIDKFIKPNILGQNIKSIMFQNSFNQLLLPGSLPESLESLVLEGDYNQPIGDNVLPKSLVTLVFGSKFEQPLLPNIIPPNVKSLSFQRGYYNENLLHWIPCSVQEFTIGSNDTNINNPLFPVEKISLSITKLIIGDNQPIFSVKQIPSSVTFLKLGPCLTYQCIGPNVLYLETSKDFDQPLHILFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PS42</accession>
    <name>A0A8J4PS42_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006514</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000304">
        <property type="protein sequence ID" value="KAF2072171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PS42"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2072171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="69" mass="7630" checksum="7964940D68A472FA" modified="2022-05-25" version="1">MIIKNLASFSLNNSIANQSNQQINSALLSSTTPSGLNQSQCYYIISLGGPILWINDRAHVHMTYFGHPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSA2</accession>
    <name>A0A8J4PSA2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Amine oxidase</fullName>
            <ecNumber evidence="6">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_005127</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a secondary aliphatic amine + O2 + H2O = a primary amine + an aldehyde + H2O2</text>
            <dbReference type="Rhea" id="RHEA:26414"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:58855"/>
            <dbReference type="ChEBI" id="CHEBI:65296"/>
            <dbReference type="EC" id="1.4.3.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the flavin monoamine oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="6"/>
    <dbReference type="EMBL" id="AJWJ01000196">
        <property type="protein sequence ID" value="KAF2073578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSA2"/>
    <dbReference type="OrthoDB" id="5046242at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097621">
        <property type="term" value="F:monoamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001613">
        <property type="entry name" value="Flavin_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050703">
        <property type="entry name" value="Flavin_MAO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43563">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43563:SF1">
        <property type="entry name" value="AMINE OXIDASE [FLAVIN-CONTAINING] B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00757">
        <property type="entry name" value="AMINEOXDASEF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="7">
        <location>
            <begin position="16"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="38"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="448"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601613-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2073578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="536" mass="60517" checksum="66A19E1394A7DB0F" modified="2022-05-25" version="1">MGSICQYDVIILGAGVAGLRTAELLLQTDKSLNVLVLEARDRVGGRTNSFQHNNHSYDIGGQWVSPHQKNVMTLIDEIGLETYSQNTNGTKVMEFAPDNRQTFSGFIPPIGFVQALKLQFIFWKLEYMMRQVPDMGVGLDLNKDYSKAREWDKISCEQWINDNIASEDLKNLFRVSMSAIFCEHPKNISLLFMLMAFKSIGGIERALSVKGGAQEKKIYNSAYQIAMFLEKRVNELSQEPRAIRFNHTVSRVSDYDSYPVKVECDNGESFTCKYLVSTIPPPLCKEITYNPPLPKARQTLIDTASMGKVIKFLVFYESNLWAHHSNITKYSGEVISFGPGVSFVYDATLENGSNPCIVGFFVGENALNWSKKRSDERKTHVTDFLFKTLGDGKDSRYLNPSEYIECDWSKEEFSKGGYSIVIPNKTSFLDYKDSLFKFIGRMHFAGSETAKDWVGYIDGAVGSAERVANEVLYQINPCKETKVKTGVIDYNDSAFADDKNQKSVLSTIATNYKHIINVGIIFIATYKVMQYIAIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSE2</accession>
    <name>A0A8J4PSE2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Type A von Willebrand factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_006036</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000260">
        <property type="protein sequence ID" value="KAF2072657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1729737at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003891">
        <property type="entry name" value="Initiation_fac_eIF4g_MI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013694">
        <property type="entry name" value="VIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45737:SF5">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45737">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02847">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13768">
        <property type="entry name" value="VWA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00544">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51366">
        <property type="entry name" value="MI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51468">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VIT" evidence="4">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="315"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="MI" evidence="3">
        <location>
            <begin position="1488"/>
            <end position="1611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="634"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="938"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1000"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1284"/>
            <end position="1486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="634"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="664"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="945"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1284"/>
            <end position="1296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1306"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1360"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1375"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1396"/>
            <end position="1412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1416"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1432"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1454"/>
            <end position="1473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2072657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1799" mass="197493" checksum="69713D807A657955" modified="2022-05-25" version="1">MYAGLWTKNGEQILPSFISINAKVVDLAAQIVFRKTYSNINKYFSTLNTINNTKEEDATFKAFLNTSMGVLCGFEINLDGKKITSKVVSQQDAMRLFEKNQSTDIVKEAEFTNSDPNIEEDFFLCKINNLSQYKELEIIITYATEMSTQGEYLLMEFPTSLTTIRSSQYFESIDNNNTGSTAATTTTTPAPNTTTTGTNSLSLSSSSIQVDQKQGLLIEMDLEMPSKISEIISPSHAKSIQVEYYTHNTGKVTYKDSRSIEVVNQSDLVILVKLENPEKPFGFVEESADGSTALLIAFYPKLNDIQKQQSAIENELIFLVDCSESMAGYNMKHAKKALHMFLHSLPKDTLFNIISFGSSYQKLFPRSVKYDNERLQAATKYVENLKTETSGDTNMLSPLKDIYSVVPTCSRKIFLLTDGRVSNTGPITNLVRENAHNTSVFPIGMGEFVSRQLVYSIASAGSGVAELAIENETIESKVIRQLKRALQPAMSNIRVDWGTLSSNRQAPRDLRTLFLGDRLNIYNILDKGVKIVDDTVKLLANGPNGPVEFPVKIKQEYTKQGNLIHNLAAYNLIQDLQDQIFESNDQHEIDTIRQKIIDLGIKYGLATNYTSFFSVEQDEIDNLNNSINELNNNSNNSNSINNNTNNNVDSSVNVESNNVNNVSIPTELNNSTSPSKSNDKLPSISRLQPIESAMISLRGSASGIPTSGSSSGIPFYNPAAANTHINNHTINSAPIPTTGAANMTPLKMSALSKSSLRVDSPEFVPKSFASLNANSIPFVPGSPMKPAATTVTSPTTPLKPAVTSPVAETKPAVVTPSVAAVETKPVVAAVAEPKPAVVTPPVAAVETKPAPVAAVETKPAVSEPKPVAVTPAPVAAVETKPAAPVVEAKPVAEQPKPTPVVEAKPAVAAAKPVEPSVNSIQSTLANFGFQTNAPAKSAAVESKPATQVSTQSSSTAATTQADKKTPAVTNIMPQSASKRIYNYDYLLSFRDSNTKAPDSLKTTPIFSNGPTSGSGSGNKAHFGGKKGRGGFRGGDHGAQSNQPVTVPKKIFSQDVQGVHSEAFKTFKFNLNRITMDTYSNLIKNIDEIKVPDEECLTGISKILFEKAIVDQKYSAVYAILAGHLDSSYPQFGGISLKRAILNNCQEEFGKVHDRSTFDTLSKEDREEKEFMIKRRVLGNIKFIGELFKHGVLGEKITKQITTNLILNTEATLSEESIECLVKLINTIGKKIDEADKTTTDEYFRRIYNLSENSGVSSRGRFLLMDILDLRKNKWQTKAAALTKAKKEEAEKEERFIKQHGGSSGGRGDRDGRDSKRGGDDRRGDRRGDRGGDRDGDDRRGGRGDRGGDRDRPPRGGKSSIFDKKGSKEGGWETVKGAGSNNKGGSKTPKKGERDSAFGSGSPFGSSKGSPFRSSRDNVPNAPSSSSKKDEFTSPQRGSNLFSALEDDEYDRAPSKSNNNTNTNNKSSNTPTKSAAPVEPAKPEVDIEKMEDSVTLTLDEFVEMNDVDEAIECIKEIVCPPSHLGKLFNILISKSLEKKEKDRSKIIELFKQIISVKFFSDDNIKEGLKEILEIIEDIEVDLPFSSKFISHIIGICIESELLPLGYLEESYIHLVDTGKAEEMLKETFNGIVEASDQDRLVEIYEKTENLDILKLFKLKNRSVAYLEEFYQTNFPYLSHEKAIVTDSDKADQVIEHLLMIQNADGYWELSKKLAGTLNIPFSAMTKAESADSVPNTHPDIWATCLAYSFLEINCPDERDDLELILKKISSWLNSQYESETSPKETLAEVLLLSKRFLSEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSL6</accession>
    <name>A0A8J4PSL6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Methylthioribose-1-phosphate isomerase</fullName>
            <shortName evidence="2">M1Pi</shortName>
            <shortName evidence="2">MTR-1-P isomerase</shortName>
            <ecNumber evidence="2">5.3.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">S-methyl-5-thioribose-1-phosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Translation initiation factor eIF-2B subunit alpha/beta/delta-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006016</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>5-(methylsulfanyl)-alpha-D-ribose 1-phosphate = 5-(methylsulfanyl)-D-ribulose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:19989"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="ChEBI" id="CHEBI:58548"/>
            <dbReference type="EC" id="5.3.1.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 1/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-2B alpha/beta/delta subunits family. MtnA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.23" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000258">
        <property type="protein sequence ID" value="KAF2072682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSL6"/>
    <dbReference type="OrthoDB" id="2461at2759"/>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00874"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046523">
        <property type="term" value="F:S-methyl-5-thioribose-1-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.420:FF:000003">
        <property type="entry name" value="Methylthioribose-1-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10470:FF:000006">
        <property type="entry name" value="Methylthioribose-1-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.420">
        <property type="entry name" value="translation initiation factor eif-2b, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01678">
        <property type="entry name" value="Salvage_MtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005251">
        <property type="entry name" value="IF-M1Pi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011559">
        <property type="entry name" value="Initiation_fac_2B_a/b/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027363">
        <property type="entry name" value="M1Pi_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00524">
        <property type="entry name" value="eIF-2B_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004326">
        <property type="entry name" value="PRK05720.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00512">
        <property type="entry name" value="salvage_mtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475">
        <property type="entry name" value="METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475:SF1">
        <property type="entry name" value="METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="2">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="349" mass="38536" checksum="2EE917B47750ADD3" modified="2022-05-25" version="1">MKSFKFLSIRYDNEASKLEILDQRKLPDIEEWLECKEPSDMVTYIKQLSVRGAPLIGVAASMSLYNHVRCNQGKLNDQEILEISNQLRQARPTAVNLMYAIDAMVQVGQEKKDLTADNLGKIACKIIDDEVEMCDKMSKFGASLIQPGESILTHCNTGSVATVGSGTALGIIREAHKQGKNIHVYVDETRPLLQGGRLTTYELEREGIPYTLICDNMAACLMRDGKIQRVLVGADRIAQNGDFANKIGTYSVAVLAHHHKIPFHGVAPMSTVDLNCKSGKDIPIEEREASEVQGASGSFGKVRWAPSTSKTFNPSFDVTPSELVTSLILDTGIYSQQELLNFALLKFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSR4</accession>
    <name>A0A8J4PSR4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TMS membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_004823</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TDE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000178">
        <property type="protein sequence ID" value="KAF2073863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSR4"/>
    <dbReference type="OrthoDB" id="5963193at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005016">
        <property type="entry name" value="TDE1/TMS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383">
        <property type="entry name" value="SERINE INCORPORATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383:SF9">
        <property type="entry name" value="SERINE INCORPORATOR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03348">
        <property type="entry name" value="Serinc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="279"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="343"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="385"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2073863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="414" mass="46225" checksum="2696FE865A847E54" modified="2022-05-25" version="1">MSNFITSGTTACSCFGTGVKKSTLTRIVYIFFFLLVSVVAYVMSYWTFSWFNNIDVLKVCMDNDECVGTLVVFRLTFGLATYHILLALVMIGVQNTFDTRAKFQDGWWPIKILLLAGIILASFFIPNKFFVVYGWIAIFGAALFIIIQLVLLVEFAYSLNEGCVARIEEEGHYSKRWYFTLLVLTFGSVLAAIAGCVLLLVFFGKCNINQFFIVFNMAISLIIGVLSINEKVREYRPSSGLFQSGVVMLYSTYLVYSAINSEPASMCSTFANSNPRESTIIIGAVFTIISVCYSAFRASDSNELLGSDGHSHEYESLPNLDTGDEDGRLEDDECSHVTYNYTFFHITFALGAMYIGMLLTNWSTIGGVSSSTSLVSVDSGLVSVWVKMISSWLVNLLYLWTLVGPLLLPNREWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSZ8</accession>
    <name>A0A8J4PSZ8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Kynurenine 3-monooxygenase</fullName>
            <ecNumber evidence="9">1.14.13.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Kynurenine 3-hydroxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">KMO</name>
        <name evidence="12" type="ORF">CYY_005759</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>L-kynurenine + NADPH + O2 + H(+) = 3-hydroxy-L-kynurenine + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:20545"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:58125"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.13.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="9">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the aromatic-ring hydroxylase family. KMO subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.9" evidence="9"/>
    <dbReference type="EMBL" id="AJWJ01000238">
        <property type="protein sequence ID" value="KAF2072937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSZ8"/>
    <dbReference type="OrthoDB" id="10053569at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00328"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004502">
        <property type="term" value="F:kynurenine 3-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043420">
        <property type="term" value="P:anthranilate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070189">
        <property type="term" value="P:kynurenine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006569">
        <property type="term" value="P:L-tryptophan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019805">
        <property type="term" value="P:quinolinate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000185">
        <property type="entry name" value="Kynurenine 3-monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01971">
        <property type="entry name" value="Kynurenine_monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027545">
        <property type="entry name" value="Kynurenine_monooxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46028">
        <property type="entry name" value="KYNURENINE 3-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46028:SF2">
        <property type="entry name" value="KYNURENINE 3-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0274">FAD</keyword>
    <keyword evidence="2 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0521">NADP</keyword>
    <keyword evidence="6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 9" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="11">
        <location>
            <begin position="15"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="11">
        <location>
            <begin position="300"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2072937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="466" mass="52279" checksum="00B1CD7822D63B5D" modified="2022-05-25" version="1">MMSSTTINSNNTITLMGSGLAGSVLALLLGKKGFNVRVHEKRSLEVNPAQRARSINLALSDRGIKTLRETGHLADILEIAIPMKGRMIHSIEGNQTFQPYSSDSKKHLYSVSRQLLNEKLGEHASKLDNVEILYNQTCKSININQGTYDILDKDGQSTTYSTSTIIGCDGAFSAVRNSMVKLDRQNYSQSYLEHGYKELCIPPGENGAFLLDKNSLHIWPRGSFMMIALPNIDGSFTCTLFFPFDGELSFKSLDSKEKVHQFFTQYFADAYKLMPTLLDDYFSNPTSSLVTVKTSPWNYQGKCTLVGDAAHAIVPFYGQGMNAAFEDVLELVNCFDKAFPKGGQSSQLSNETLWECYNAYQNARKANSDAIAEMAIENFIEMRDSVSDPMFLFKKKVEHLLEQTYPNRFVSRYELISYSTEPYAYAQKIGLLNQQILNQLVKDANFDISKIDLALADKLITQVLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT32</accession>
    <name>A0A8J4PT32_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006653</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000317">
        <property type="protein sequence ID" value="KAF2072019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT32"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2072019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="589" mass="68574" checksum="A55C074E6093390B" modified="2022-05-25" version="1">MSTTSSLSSVSAPTIVQNKVIVSRIFKEFDLKCHVNVIPFVCKEWNKWAKDIWIQFFFAYLEGKVNDTASFHISLMMGNNIIDFKRLFKSLFTYDYFSAIVLPEPVYYNDFKMFDKYTRPPVTAKLAVLDKMHYVTNVLKRVMREKTPFSVTSSTAKGHKYQYQRRDHPFTDSVDHCVQTTTLYHMFVSRQKSVARVDSPCRVTSDFVVFNCYENSPRDTSKTRVVSSMKAVRFIVDVEYDLIVEHRVLQAALIEIRAKKVDLFQENSSVEDLFSQDTVTGYTTISNESFRASSMSSYKGLPVVISDEQHQSNYLFIGDSGIDFEQQSNERVDQVAGDDDMQETEEIDEFELDDKESRNHHRLDYLLAYYTTQHDDQCSIYLFIPRDRVTPIADFLDIRGSELIEMVSMFLSPRASPNPSFSQFSRAEAIYRLYSKILSSGNKGLIYIAKEILGYFQYYSFDFTRNGSRLFKLTCIPTNGNRNTEYRVRLSHDQHGYAITTNFCPKNSLLDAIDESYLPSIPVPYLDVDKIKELHALFYFHLFQFDRYIDYQTFYNINSRTNRVSYKNYNQYDFIDSDDDDDYDGGGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT78</accession>
    <name>A0A8J4PT78_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006593</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000311">
        <property type="protein sequence ID" value="KAF2072094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT78"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2072094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="124" mass="13934" checksum="BF8227FE582CD14A" modified="2022-05-25" version="1">MQNMTISSPNPAYFTCNLCDENFKAELLYFHIPKCYFEQAQKNGLDTFCTCPEHKGSRFHSCHDMACSSKNNNNNNNNSNNINSNNNIHTSSGIQNSNSNCSSNVNSKNPMIDPNGINYLAYNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTM9</accession>
    <name>A0A8J4PTM9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005514</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000222">
        <property type="protein sequence ID" value="KAF2073161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTM9"/>
    <dbReference type="OrthoDB" id="20865at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018482">
        <property type="entry name" value="Znf-C4H2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31058">
        <property type="entry name" value="ZINC FINGER C4H2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31058:SF2">
        <property type="entry name" value="ZINC FINGER C4H2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10146">
        <property type="entry name" value="zf-C4H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="258"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="149"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="54"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="258"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="279"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="289" mass="33207" checksum="0ED8BAABC3B138AB" modified="2022-05-25" version="1">MDHSNTDTESQQSNDGFLIATSSAEDAGNNNNDNNDSNLQQTNTYSVKKEKETSNSNSNSGSNDGNSSIDHNVNDINTHIAMTLSSMKYENNSNNNIKIEQQQKDDDNNSNNDNIDIIKNNLLVLNDIKDKEKDYNQIKDCVLKQMDLIDKENNMIDELHMERSQLIQEFKKYELLMKEIQIDLSRVDNVLAVSKKEKEKIQADLQQLQDMQLYPLKDSIDSQRKSIGLPTLPSIQEEKDRLNAEYLRDRLDKHILQQQQQQQLQSSFQSQSHTYTGAKRGRKRSIHTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTN5</accession>
    <name>A0A8J4PTN5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000190">
        <property type="protein sequence ID" value="KAF2073673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTN5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="83" mass="9759" checksum="7A7D07642FD35663" modified="2022-05-25" version="1">MEFNNLEYLANTIVNLTNEETDENDSLKSSTEITKPTDIQTVSIYQNNYNRSLQSQLYPNYNNRYHSINSSFIEELEDMQTPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTW6</accession>
    <name>A0A8J4PTW6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005656</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000231">
        <property type="protein sequence ID" value="KAF2073040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTW6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="76" mass="7569" checksum="89DCD1CB0423A33D" modified="2022-05-25" version="1">MIFSILSSLSPSKSVCSVASSSSSSGSFRNGLNMNAGGATTLNANVNANVANGGKLVDADIHTRGAVNSDIKAIVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU55</accession>
    <name>A0A8J4PU55_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_009128</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000642">
        <property type="protein sequence ID" value="KAF2069551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU55"/>
    <dbReference type="OrthoDB" id="2414723at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18316">
        <property type="entry name" value="BTB_POZ_KCTD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045068">
        <property type="entry name" value="BACURD1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025574">
        <property type="entry name" value="Nucleoporin_FG_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING ADAPTER FOR CUL3-MEDIATED RHOA DEGRADATION PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145:SF8">
        <property type="entry name" value="RE57120P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13634">
        <property type="entry name" value="Nucleoporin_FG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="6">
        <location>
            <begin position="57"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="165"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="272"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="426"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="19"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="278"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="309"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2069551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="638" mass="65502" checksum="C3700D263D3761F6" modified="2022-05-25" version="1">MTKSQETINQITKSIQGLTQKLNVEIDTLEQEIKQIEEKNKQKLKVINTITNTTSSEPITLSVGGVRYQTSRSTLIKVKNSFFYDLVFEQDNQQTFFIDRDGEIFKYVINYLRDGCIESIDSETEFVNELKFYKVVSKDSTVSSIFDDQSKEKKVVEDIKSLDLGSNTSTPPTPTTPTTFIFGTSTSSSGKSGNDGNGTPKGFGLFETFASKASITSSTPSPSSPFVTMNPSSSLFGGEMIWECPACEHKNDGKKTTCCICLITKPKSITYKPKSRATPLSGSFGNAPSASVFGQKSTSPTKTSSLFGPIPTSTSTPEPTSSGLFGSSTSTPAPTSSGLFGSNIPSSTSSPFGGFGRATARPVGTGAPPYNATQSEGCSFRSITAMDAYSSKSFEELRLEDVLNKKTGAAATITSPFNIGGATSSPFGASSAPSSLSTPTPTYNGLFGSSSTPTPTSTSSGLFGSSSSTPTPTSTGLFGSSTPTPTPTPTSTSTGLFGSSSSSSTPTPTSSGMFGSSSSSSTPTPTPTSSGLFGSSSSSSSSSSSSSSSSSSSSSSSTPTPTPTSSGLFGSSTPTPIPTPTSSGLFGSSSSSTPTSTGLFGSSSTPPTPTSTGLFGSTNTAAPSSSGFFGSSFGRSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU71</accession>
    <name>A0A8J4PU71_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006013</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000258">
        <property type="protein sequence ID" value="KAF2072679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU71"/>
    <dbReference type="OrthoDB" id="20975at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13270">
        <property type="entry name" value="PROTEIN C20ORF116-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13270:SF14">
        <property type="entry name" value="SEX DETERMINATION AND DOSAGE COMPENSATION PROTEIN SDC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="26"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="204"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="473"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="623"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="228"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="259"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="273"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="316"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="331"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="504"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="534"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="888" mass="97470" checksum="50E0DCD9A0E87106" modified="2022-05-25" version="1">MDTSDYMLNINPLQEPSNLFMNLGFDQQQQQQQQQANQNCNNSKPNNNNANVDTPSTFFNDSSSPLNMLPSSPPLIQQQQSTQQQMIYPLNVKTETDSAITSPIITPTNAPLNPAVILTSPLTTTTTTTTTTTISANKPPLSQIYQHHHGSQQTHEHFLKLLTQIQKHQQQSTSFQTNHEIISAHTAAAAAAGVSTTTTPIIPLFESNTESPSSTTTTTSTTIESVPKKSTTVATRANISSPPNSPRKGSTRNSKIKIKTQQVHQQIQQHQTSPLLNSNTTTGACSSQSTPNLSPTMATSNVPSLNNFSLALSPPNTSPPQSPPLPPPLPNGDLIGPPNKKLNSSSSSLTNSHKLKSSNSGGNSSHKLNSSGNFDTKDGANSIATSSNSVNVNEDKKAVCLSFLKYYFNVNQNNTTPCLRRSLILSLYVNKIPQESRYRRPNDMANLCVVLYGYLFASLNESTIKEFISSHSNFVQKSKKDEKRKKRKRDRDDEKSNPLSPRGNTSNNNSATNTPSLMSSTPSNSNSIEPLDFNNWNNSANDQNSTTDDHMNNNSNSHHNGLPVISECEADEEETPVSLHDAFINCLKSCTLLPFLEFKPVSEIPCPPSDLPVSDSFYTYDDLIRWENELKDLRLSVKQQNQQQALANAQQQQQQQQTLAQQQQQQASATAATNQNNIQQQFQQIQQQQLQSLVQSPILSTQPLSPPSLSNSMMNIHYSAITPTISTDGIQLANNIQSSPFGLLDGNNSPTALTTSPTSTLNNSNSNWNLSDPMSGVLVNVYENIDSFIIYVAIPHSQNNTLKVTVNQYEVIIEGVISIPDTIYLPNGQEMQVPVSYPPTLQSQEFLEGSFQKHIPLNSPVSSSVVGKREGIIVIIARKDHQLHQPTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU98</accession>
    <name>A0A8J4PU98_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005115</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000196">
        <property type="protein sequence ID" value="KAF2073566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU98"/>
    <dbReference type="OrthoDB" id="19660at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="862" mass="98522" checksum="D9A49EA19CCD6525" modified="2022-05-25" version="1">MTVYDGEAIGYVDYWKHEFENQRKKFIELSGVVYSASSSNSISTSGGITLDPNLSHNQERYADNLFDTWYQYAIRYAQQTTLDENNQLIINNNDQDRYDLECETIISIKVLVDFYIIKFLLNETSIQDSVIRRFMEQYRKILTILQTKSKFTILLCCEFERSTNTLKDMIKRSIEFFLFLLQRNTSSMTKKIECINSIQTSIDLGNCMIKIGSVNLDVDKFYFDINIVPIVQFTSSREISDIYRLLGFKILETIPSNIPLEENNIKLRGLTYLASLACDELSFGFLWSLGGLTQYPHCSQIIINRLPSATFDVAKSLILSIPDALKSSDHDLLVNQAKGLKVVFNIIDAYQNKLKTIDFDQDTIHYELIDVYNILLQQLPNEAQIHDKMFKDIGCTEETMAVLLNCSFRFDIITWQQCLLPYLREEILKMRLQGVSGIQKEQAVIDCVRECARIFYLDSVNSISFENSLALGKALLQTIVEAMRHGYIETERFSLICSIILTNHLPQSIEWIPEILTTPVCHNHKTSRAVFDIFHYLFGYKEVEESTTLQSLKAENISIIFKKIVTIPTSPAYSLVNSLLTFKLAKSFLTENHYLISMLLDTLASMIEYNFVFDKDQAESITSLVKNLSFVIHPSQAVKLFSLGVKILGLIDCHPSSFGDETLSKIVNQIPQSTPVSIQDVHMGIERGGQETQCALCSFVPQICILEKNPVVITESLKCFATRDDLIKEHYKTIVKVCKDTVVDSIYDLELSLSAAPLMPIICQIDVNMIPECILILYKAIKLSCLNDPTNQPNSIPIKHLETVISTIHNCDQLLPILRSSPLLLSVLSKLKKYNSTFKQQIKELDKRGKLRRNKQKEYELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUC3</accession>
    <name>A0A8J4PUC3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003699</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000119">
        <property type="protein sequence ID" value="KAF2075003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUC3"/>
    <dbReference type="OrthoDB" id="19040at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000016">
        <property type="entry name" value="TBC1 domain family member 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF25">
        <property type="entry name" value="RAB-GAP TBC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="593"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="412"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="699"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="180"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="706"/>
            <end position="718"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="718" mass="81261" checksum="4C2049C57B189F4E" modified="2022-05-25" version="1">MDTTTNSTTTEEEELTTRPATKLHTPAAVSIPLTTTTTTTTTHATPMSNGMQENDALDGDIATQGVTISTTSTTIPSIDTTTIEQRENNDFQEIVSLIKENNSNQLDRVLNEIKSRIGAEQLKELLNTRIPDNNLTLLYYALVLDCYNDLVLLLLLNGSDPNQMVTPLPQASSNNIVLESPESSSSSNSNNRAPSPPPTESSSTINSKAYANAKTDTVEEFYKKPASLEAAIHYTVRHGDSDKMNLLIDNHADKDILDSVQRTPLILASSNGRMECVRCLVLAKCNITLKDILDKTALHVAIEQGYDDISATITSNGGDISLRYHYKPIRSSRLTFSSSADYTDFNDVEILKRVDRYGNIKDQPIVKDEYTRKKEKKSVEKELSRCSKWLSLLKKWNYSKITSKVKSKSVKGVPPRIRCEVWSHLSHSNLQEALNPNLFETLVNQHSSSEVVIDLDVNRAFRGNIFFRERYGVGQVALFNVLKVYSLYDTTVGYTQGMSSIASVLCMYLPEKEAFWTLERLMNREKYSMRDLFLNGLPKIHKTIFVFDKLFVHYLPNLSKHFESINLGSMIYATKWFIIGYLDSLPFNVSLRIWDLIFVYGYNILYSVAICLLRMFEKQFLGKAFENCYEVFRSFETYQFDEDEFINFVIKHKIKSKKIEAIEKKYVPVGGGSGSQQINSPPPAVSLISSADLIQNSVPLLPNNKKDKKKRFSFFQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUJ5</accession>
    <name>A0A8J4PUJ5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Mitochondrial substrate carrier family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_003573</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000113">
        <property type="protein sequence ID" value="KAF2075138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUJ5"/>
    <dbReference type="OrthoDB" id="1747031at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990542">
        <property type="term" value="P:mitochondrial transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045315">
        <property type="entry name" value="Mtm1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45760">
        <property type="entry name" value="FI19922P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45760:SF2">
        <property type="entry name" value="FI19922P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="57"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="167"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="269"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2075138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="369" mass="40609" checksum="7B94A670EE779527" modified="2022-05-25" version="1">MNPNSLPPPLTPLAPLAPLSGNQYGDSTNNNNNNNNNNTNIPNDSNKKFLKRGIDEQTIGMLMLASIVGGIVTSAVVTPLDVVKTRLQTQVDKHHIPSSNSTIHNANKVRFKGTLDAFSKIYKHEGIFTLWRGLAPSLLMTIPSTTIYFTSYEFLKEKIYILNKDQGYYTVPLIAGTIARVISATITSPLELIRTNSQGVVTENHKRLNSITLFKDIIKNVGVKGLWRGLSPTLLRDVPFSALYWANYEVIKAKMMISRDPSYTRHTKSPFLTTFVAGATSGTIAAVVTTPIDVIKTRIQMTVQQSKVSTSASGTKPPVVSTSAIYHFKEIIKQEGWQGLTKGMVPRVAKVSPACAIMISTFEWIKSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVS0</accession>
    <name>A0A8J4PVS0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase M20 dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005766</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">The Mn(2+) ion enhances activity.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000239">
        <property type="protein sequence ID" value="KAF2072909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVS0"/>
    <dbReference type="OrthoDB" id="6119954at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016805">
        <property type="term" value="F:dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071713">
        <property type="term" value="F:para-aminobenzoyl-glutamate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046657">
        <property type="term" value="P:folic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017439">
        <property type="entry name" value="Amidohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052030">
        <property type="entry name" value="Peptidase_M20/M20A_hydrolases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01891">
        <property type="entry name" value="amidohydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30575">
        <property type="entry name" value="PEPTIDASE M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30575:SF3">
        <property type="entry name" value="PEPTIDASE M20 DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005962">
        <property type="entry name" value="Pept_M20D_amidohydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="2">
        <location>
            <begin position="230"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR005962-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="432" mass="47305" checksum="703FCF1CD526469A" modified="2022-05-25" version="1">MNRDDEIDYKSIVEWRRNFHRYPEVGWTEFRTTVTLVTLLREMGLLVHVGTKVINPSFVMGRDTKLVESAIESVKKTLSLDQQKILDEMQGYTGCVAVYDTGRPGKTVALRFDIDCLSVSENKSNDHYPVKQGFVSERDGFMHACGHDGHMSVGLGVAKWLAEYGEKSGLSGVVKLLFQPSEEGVRGAKPMAESGIMDDAQYFLGMHLGFIAKTKEIVVNPNNFLCTKKYDFRFFGVPAHAGAEPHVGVNALSGACNAATQMLGIPRHGKGMSRINVGVIRAGTSRNVIPAFGELQIEVRGETEEINTYMANQAERIAQGVALGHNLRLESELMGEAVDLNNDQEMVDMLSSLVNQDSSLINVPSRSFGGSEDATILAKRCQSNGGKSLFFVVGSNLAGGHHQSNFDFDESSLLIATKLFIASLLTLCESNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWL6</accession>
    <name>A0A8J4PWL6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">IPT/TIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_003307</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000102">
        <property type="protein sequence ID" value="KAF2075383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWL6"/>
    <dbReference type="OrthoDB" id="20300at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052014">
        <property type="entry name" value="Dictyostelium_Tiger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31341">
        <property type="entry name" value="IPT/TIG DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035221334" description="IPT/TIG domain-containing protein" evidence="4">
        <location>
            <begin position="21"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="806"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="5">
        <location>
            <begin position="269"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="5">
        <location>
            <begin position="528"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="5">
        <location>
            <begin position="705"/>
            <end position="786"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2075383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="845" mass="92283" checksum="0405F2A370535593" modified="2022-05-25" version="1">MNKGIIVFLFLTLVFKTNWCIDPPVQNGTQFSFSKNSQVVTELRYLNETGYKLLPINDCSNDPSTTVCTFELRIIAKVLLVQDNFLVYSGGSYIDDWSVGLNNNYIDPVSDISTLTQNYSITVFFSQIGLMTESYVYIIQNGISTYISLKNENPNEFTQYILPNFPAGSGVGTIRLSNFYERQFNHSQPEIDSLSSTLTTTTITGRNFFTWINQTSIWINNIALPQSSIVSVGQNQIALKVPKNSVGSFDYKVSISGIQSQIKTLNLYPIVKSITTTLKTGGLVTITGDLLFTKRPNGDPTSISIQIGDTATITSATNLQTGDINHLVFTIPPGDKDYQKVTVTIDGFSSQETSVDFSYEVPEISSITQSGTKLIISGTSLLSKSIVVVNNVTLGNLTYIDSSSASIELPPTTKDGSLYVRTEEKISNNFPFTLKPFLSSLSGGSTSGSIITIYGSFLKLQSTNTLVSTDKLLSCINFVQGDGSYITCSLSKGVGKNHSMTLTIGSTQMSGTFSFNPPVINTFSQSFDEFTLSGNNFGLYNNATTIYFGGSSYKPTFESFDTLKFKIPASSKPGPVYVVVAAQTSLEKPLAIGPAIQSVLFPDGGNEIPTNSDIDSFGNKLPYYTKKNITIQGLFFYPSQSYQVTLKNDKRIITCENATVVDDQATAIKCTPPWSSGIGYIVHVTTGNFFATLDTPFNYEKPTIFNSSTVSDTGGIVTIFGSGFAPQSGVMSVNIDKYKCIDPYGTSSQTLECFIPKFDNNTDVPRNNLTIYLNVNGQSTFLENSFAFNVTALPVMDSENAPTAKWLVAAILVPCLAAILAIVVVSIIFVNRYKKLKMIKKELRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWM0</accession>
    <name>A0A8J4PWM0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003562</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000113">
        <property type="protein sequence ID" value="KAF2075127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWM0"/>
    <dbReference type="OrthoDB" id="10255522at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053019">
        <property type="entry name" value="GATA_zinc_finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23353:SF23">
        <property type="entry name" value="PROTEIN HAIRLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23353">
        <property type="entry name" value="RAB-GAP/TBC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="24"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="87"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="880" mass="102236" checksum="4A126384AF85B0F8" modified="2022-05-25" version="1">MDNKQQNHSQHSLEQQVKDFIPISVQSKEKSKRIKNQIQDLKSENSTLLNETKKFKNKLTEETGVAEMLIEEAQLLKKIVVDKDSEISSLSKTIKEQSDNIDSIKKELENEKMLLEKEHEKMLLEKDLKESVQQSAKNLEIEIVGLKDTLLLESQNLQQTKQLLTEEKEKLAQEITSHKQDLDRLNQEIETNQSTIKELNETIQSLNNQSEKMVGEIDELQNNINNLTQQINSLTSEKEQLQLQLNSETQKFENISKLYDQLKENHEKVLLELEDVLNTKKELESSIEKHKETITSLESINQQLESEKKTLNETIEQDKESHRVEIENLENEKKQKDHEKQQLENNIQLLVKEIDEKSSTIDELYQIKDNLNLEIECVRKSHLKEMEDLTNQIQTQNQQIIQITNQLGEIQFLKDENQQLGQQIVQLDETLKSKALEIEEKSNSISTLEKDIQEKEQELLASSRALQKAELEKEALKDENESIKTLVSQQENSNLDQQIELQQQSKKDIQNLNLQVMEKNNLIEDKDKEIQELNDSVEKLKQDIINLEEKVEEEKKNRAHDQEKFDDEKEKINRKHIEDQIDKELVAEKVIADLKAIIDNISNERDTVQREANNLANRLSNQVDKCQELESNNVEYQKEIASIVSTRDNLIQEVEELNLKIANTPAPVVVESNTNNEKIEQLEKDLLTLREKYKADLDQFSKDKQQWESNRWEIVAELEQEKMKSKKIQESLMEEKRVMGVVSKTVSKLLSDNEKNSFDSMTPESFYALLEATKKELTEGLHNKKSYYDDEDLMGQLLMEREARRLAESQVEHFAVLLEKSKYEISKYKEIINNGGGNNKSSGFFGNIMKSINGDNTGNNNNNGFLKRTFTGSNNLGRNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWN8</accession>
    <name>A0A8J4PWN8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase</fullName>
            <ecNumber evidence="14">2.4.1.101</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">CYY_005398</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + UDP-N-acetyl-alpha-D-glucosamine = N(4)-{beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11456"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="Rhea" id="RHEA-COMP:14368"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:60625"/>
            <dbReference type="EC" id="2.4.1.101"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.101" evidence="14"/>
    <dbReference type="EMBL" id="AJWJ01000214">
        <property type="protein sequence ID" value="KAF2073299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWN8"/>
    <dbReference type="OrthoDB" id="17286at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003827">
        <property type="term" value="F:alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000252">
        <property type="entry name" value="Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004139">
        <property type="entry name" value="Glyco_trans_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052261">
        <property type="entry name" value="Glycosyltransferase_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10468:SF6">
        <property type="entry name" value="GLCNAC TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10468">
        <property type="entry name" value="PROTEIN O-LINKED-MANNOSE BETA-1,2-N-ACETYLGLUCOSAMINYLTRANSFERASE 1/ALPHA-1,3-MANNOSYL-GLYCOPROTEIN 2-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03071">
        <property type="entry name" value="GNT-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF2073299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="631" mass="73262" checksum="C492A613979B7565" modified="2022-05-25" version="1">MLNKLNYYNSNLLKHKTNKKKIIITVCLLVAIYYFFFLKTGSSQEIGKNKDKILFQDDEQIQLFTMDDKELFSRKNGWNYDELENVINANIADMNQYNYEEKVSKVVWLDHIRPVNENLLRIKNNGKSKNFQNTNSIQHPDIAIITSNKPEYLERLLYNLLLVSNANVQRIHVFHDGYSEISDILKKYGVNPELHVSQYRHVANLKTQEEKLYYMNSHYKEIFNYVFNNQSMDKVIFLEDDLILSPDALKYFDILSNLMDVDPSIFCISAWNDNAFKFQDDLMKTFQDSRFTFYRQDHFGGLGFLLSHQIYTERIEPIWDKISTEPWDTIIQSAMSPGDQCIYPEMPRSQHAPNIKRNYTSVLDHQNEVDHWGFYKMYSDYENIASNVLDLSNLLYHKYDARLRSKIQNATEIDSFTAIPVLFSSTNDFVIYTKAISNSDSRWDKVINDLGIVGRGNGNIVRGIYKGVVETTYMGRNVVLVGEYSVFNNQTLRKTKPKFAVKDIPMPAAESLSGDPLSNAFYYRLKNDAALDLSIAHRGKSCEEHCTDKKQLCKEADMVLLTQPNAVLLMMEDQCESTEFSDSSSESSYLPFHMNDDTCLIPNDFRYLTCTYKPSAQLFGSRICLCRKISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWP1</accession>
    <name>A0A8J4PWP1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fatty acid hydroxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_003661</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000117">
        <property type="protein sequence ID" value="KAF2075051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWP1"/>
    <dbReference type="OrthoDB" id="1658724at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="6">
        <location>
            <begin position="135"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="274" mass="32424" checksum="5762A6D2E7766B14" modified="2022-05-25" version="1">MDVKTILSFFKSTQTYNVEIDNPTNIVEYAWIWVLQTCGEFGLFLGFYLAMAALYAVGGFIFHLFDKYKLFPKRKLQATKYPSQADMTRCLNNLIVNYIVIILPLGIVSFPFSKMLGLTYSLPLPSVWRFLLDIFLCLVGEDFFHYWMHRFFHTPWFYKNIHKEHHYYSAPFGFTASYAHPIEVIFLGFATFAPVFIIRPHFFTFYSWFIIRQLDAVLTHSGYDIDIFPFNFMPFYGGTSFHDYHHKEFTCNYGSRFTFLDKVLGTYKEKKVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWU0</accession>
    <name>A0A8J4PWU0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Myb transcription factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_005329</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000209">
        <property type="protein sequence ID" value="KAF2073359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWU0"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019185">
        <property type="term" value="C:snRNA-activating protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042796">
        <property type="term" value="P:snRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051575">
        <property type="entry name" value="Myb-like_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46621">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46621:SF1">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="72"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="79"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="125"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="129"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="181"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="44"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="238"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="344"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="411"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="241"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="298"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="366"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="414"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="427"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2073359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="562" mass="63258" checksum="70FBCEDFE295C556" modified="2022-05-25" version="1">MSESILITSYPPKYHEHISPQFQSEKMMMYNELPSLAMDDADFSDNEYEMSPKGDEVPSPSKKRGRGAIQNGIRRSPNKWTKEENQKLFELVHMYGEKKWKRISAEMGGQKTGAQCAQHWKRVLSPDIRKGPWDEDEEELLLRLVNQHGSSWKKIAKRICKRTDIQCRYQYLKSLQSREVPWVVKEDEVLVKKVQEMGDSLSWLEVSDFLAKLKHTNTLRTALECKTRYMQLTNTQYPPTLSFNNLNTNSTPSSLNSSSSSIFNNNNSNNNNNNNNNNNNNNNNIKNSHSLSPLSHLIDSNNSSPSFESCNEEQSSSPPTTNPYYKPQSLNNSVIPIYKPTPHKATISHLTSPNSSPAHSPPLSPLPSSENNNCNNNSNNNNNNNNNSKPIFSSSSSSTCLKNNSFSINNLIDSHSNNSNSASSSSNEKDEDHEPQIKKVRSNGEYYYQPNIIFKQQQQQPTFNSDTSSDDDINTTKKINQLDNGIKSSYFSISNINNTNVNNNKINFTYNSSGSTSPSSSPISSPTHSPSLQKLKATSFDFFNLESLAAIASSKRHIEIAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX36</accession>
    <name>A0A8J4PX36_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003398</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000105">
        <property type="protein sequence ID" value="KAF2075321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX36"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035191720" description="C3H1-type domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="14375" checksum="4F9CE9787F50CF5A" modified="2022-05-25" version="1" fragment="single">MKFLILTFIFCLISVSLASVDFNRPTKPSSCSQLNQEWKCRLAGHPCYWNGKCLSLIHGTSDRSIFLESTSDKANLIESTSDKANLIENTSDRAIFLESTSDKANLIENTSDRASLVYMIESTSDKANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PY59</accession>
    <name>A0A8J4PY59_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002369</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000065">
        <property type="protein sequence ID" value="KAF2076313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PY59"/>
    <dbReference type="OrthoDB" id="1062969at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="439" mass="48074" checksum="65D1D4EF9888D1B1" modified="2022-05-25" version="1">MTIRKPVRLNHPDDLPNVYQGRLRIIGTLGESVKKYRALHAATGLTSLSSGTNNVTPNKQPLLMTNDENKAAYMNNNFGKIKDIETKAQNDLQPVYTNISNNPDTAWVEVEDTDTGDQFYMAWSSSGSVWSFTQSINPVSFENKKIRASQGDTDTKYEALVQFGTYSQSGSVAGIHSYNIGLGTMAVESVIAMMLAKTISGFVSDGLGFLVSAFAERLGAAAAEVGLEVAFVVPEFVIGAVAACLCFAIVFVGLAYLWNWINRQYTIRVQIFNWDTNNDWQVPSQALSNAINPGKDNQTTEMGIKLDKMVDAGAIVYPPGFQGVKTLDNVVYYCLIIYENDHTFAQGCSFAVQMQCNGSTTNGSTYAFQCPRWSDNGHYMVGQVIDANSFLETARSNWTSNMSLNVTTANNIPVNSTMDALSGADNQLYNINIHINKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYW1</accession>
    <name>A0A8J4PYW1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_002199</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000058">
        <property type="protein sequence ID" value="KAF2076521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYW1"/>
    <dbReference type="OrthoDB" id="270167at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01448">
        <property type="entry name" value="TST_Repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01449">
        <property type="entry name" value="TST_Repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000001">
        <property type="entry name" value="Sulfurtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045078">
        <property type="entry name" value="TST/MPST-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364:SF27">
        <property type="entry name" value="SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364">
        <property type="entry name" value="THIOSULFATE SULFERTANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="64"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="217"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2076521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="336" mass="37970" checksum="8B8783C903E5EB0C" modified="2022-05-25" version="1">MIKSILPLFKSSSPSCSSKLFNNSRSIYLFNNSNNYNRNFTSTSNKMTDARKQLFVSPQWLNDNQNKVKILDASWFMAHEKRDPRKEYVDKHIANSKLFDIDEICDKTVKLPHNLPTAQFFEKEMQRLGVSNDDHVVIYDSRGQYIASARVWWTFLVFGHPATKVSLLEGGLPAWEKSGYKVVAGDIVNNTDSAIQYKATVNKDLVWDKETMLANLDTKEYQVLDARAGDRFWGRVDEPRPGLRRGHIPGSLNIPWVEVITPEGGYYAKDKLTQIFKDKGVELDKKIATTCGSGTTAAVLSLGVYELGHPIPPVYDGSFSEWGLPEHKLPLSCSND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYY0</accession>
    <name>A0A8J4PYY0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_003579</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000114">
        <property type="protein sequence ID" value="KAF2075102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYY0"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22671">
        <property type="entry name" value="FHA_APTX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000180">
        <property type="entry name" value="serine/threonine-protein kinase STY46-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF298">
        <property type="entry name" value="MIXED LINEAGE KINASE DOMAIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="237"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="534"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="495"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="174"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="805" mass="90204" checksum="507EF06EB689A59A" modified="2022-05-25" version="1">MSDNSNNSKQQEEEEDISTDEEVSDDLIVDLIFKSATGSTQSHRLQNGINVIGRGKPVFISDAKISRRHAEIIVSSSVTYNQLGQNHSILLRPESEAIKLTKGVSQTIKNNDSIALYEGAEPFSIRVEKIGGFTPMSFDDFSNDATQQLHDDVVHQSPLDYLHKRQPSAAVTAGGQNTTPKRTRDSMLQPSTKIISNNNDIAVEQIEKKLKKSPDSLNSSAVLIQDSLKLSINEEEFTFVRKIGQGACGEVCQYEWKGTPVAVKVIYKSLLHKDTKGEFEKETEILKCLRHPNVVLFMGTCTLKGNLSIITEYLNRGSLRDVLNSPLAAELDWNTKIKMSSDIAQGMNYLHTYNPKIIHRDLKTLNLLVDTNFNVKVSDFGLSRFVSSSNEIAKTFCGTLPWIAPEVFAGNGYTTQADVFSFGIVLWEILTHKQPQGNTATTPLGHPEIPPSCPLPFAELIRECCSRKPEQRPTFQQIIQKLKSMTTLVPSFPQTHLSTSNAPSNAQQSYNTVPGQPSTQPVDLSKWTIALSSIANVKLVSKEPDGTTILKGVYNGKPVSIKNFTTPITEAEQKQWAVLASVKSVHAVEFYGVVYNKQEHAIISEYMANGSLEALMLNTAFNLTLETTLNLALQMSESIHVLHTHTPVILHRGITSSSFLLDDQYNLKITDFGLSRFQIHENMISLAEIKGNYIYSPPELLSQGQFTPKSDVYSFAIVLWELFERFSTKVYKKPFSDIELDYDFQILNHTGKLNKRPTINEKTPKEISKLLQQCWDSEPRVRPSFDQIKTELKNIKRKLITMNQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ79</accession>
    <name>A0A8J4PZ79_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002954</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000088">
        <property type="protein sequence ID" value="KAF2075712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ79"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="34"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="164"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2075712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="200" mass="23696" checksum="16FD3B851CCA460E" modified="2022-05-25" version="1">MERVSDDDNSNTNSNRLENEKQGSSYDDSSRDTIEYLKQNIDIMKQQNNKILDQLKQEIKDIKQLQDKSILIEKELQHHNNDSVSHQIKQDDEKEKDLYFHDTELLNKNFKNMLDIINTSIGNMKQQEQQDHRIEANEYGGGKLLNKDFSRIMNSINDTIRNIKNIQQEDHNRIENDLQEIRNTIETIENDQQQQHQGET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q025</accession>
    <name>A0A8J4Q025_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001480</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000035">
        <property type="protein sequence ID" value="KAF2077226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q025"/>
    <dbReference type="OrthoDB" id="61155at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35506">
        <property type="entry name" value="OS02G0135600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35506:SF1">
        <property type="entry name" value="OS02G0135600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="284" mass="32416" checksum="3A0858976679EDCD" modified="2022-05-25" version="1">MSYKNIRCVLVWLDGAFSSFNEKELYRDLDLSFINTLVGNGCAGRLLTLPNEGDNNEKTCFTSQQLFNQILGFESNITLTNDLFKEQYSQLVLKLFSNNNDIELKGGNENRGVIDENTTPQQLFNFVESEYQKAKNCNDIITVVHIKPKSESINDRRDSLLFLNSFFKILLESREKDAHDYYINLVCSYNRGSVDIATTTHSIPIPSTFNLPKPSTQFLNGKEVNPNKQSPLFAIYNHPILTRKDETEVFNEEELLKGSNGSILLIQYLKELAFKLNKVPKYGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0J3</accession>
    <name>A0A8J4Q0J3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001921</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000049">
        <property type="protein sequence ID" value="KAF2076795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0J3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="114" mass="12933" checksum="4FADC1FA8F9242F3" modified="2022-05-25" version="1">MFVSNTLDDKYPDNFKTQTSNHHLTDLLFEYLHVSLLDWNGILQLQAIDIYNLYNFRTNMNNNNNTTKPSTTTTAPASTTSGPAKAGTWGNYANAGYDLNQIPKTEVLYRKPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0R6</accession>
    <name>A0A8J4Q0R6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Folliculin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">CYY_000873</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
            <location evidence="4">Microtubule organizing center</location>
            <location evidence="4">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Lysosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the folliculin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000018">
        <property type="protein sequence ID" value="KAF2077828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0R6"/>
    <dbReference type="OrthoDB" id="5599713at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1730">
        <property type="entry name" value="Folliculin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037521">
        <property type="entry name" value="FLCN/SMCR8_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044886">
        <property type="entry name" value="FLCN_DENN_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021713">
        <property type="entry name" value="Folliculin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037520">
        <property type="entry name" value="Folliculin/SMCR8_longin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032035">
        <property type="entry name" value="Folliculin_DENN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31441:SF2">
        <property type="entry name" value="FOLLICULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31441">
        <property type="entry name" value="FOLLICULIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11704">
        <property type="entry name" value="Folliculin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16692">
        <property type="entry name" value="Folliculin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51834">
        <property type="entry name" value="DENN_FLCN_SMCR8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="13" id="KW-0458">Lysosome</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN FLCN/SMCR8-type" evidence="17">
        <location>
            <begin position="219"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="425"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="86"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00009987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00021824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF2077828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="698" mass="79197" checksum="E4F7FD61A4F503E5" modified="2022-05-25" version="1">MIKVKIPSFVNDNTSTTSSSSSSSSSSTIPVQDKEANGNNNNNNNTTGSYDFHQSTIVLTHFCEAHGPSVIFTTQTEFSSLPPITEQQQLNEEQQQQQQDQYKKQSLKSSINLETFNDIEMNNSKDEIVISNFTEESNSTNTIESTSTPPIQPVSLSVFFQQQQSNSNNNSNNIQDGQQYYNNHLSNRVGKQYHVLINNTKETTTTTTNTTGSKCHTCTSISQGTALLSQDLINNNVYYVSTHQPSNNSYSQIRNACVRSLSCEVVPEKEGPVFISSSEGTWLSYIFKINDSRARGLKRSYGFLFLLNESSCIINYVNWIIHCFKTLAEEIKRKSEVIYEREKSQKTHNTVVMANINRYRTINLKPLIELIEDPYFFNRLHNSFSNILSQCQAKYHLLKGSTNYILSEQHDIIKDLKKVIIKHNNINNNSSNNNSNSNSNNNSNMNSSYSSNSNNNNNNMMMMMGSLTSLLLDEALFPIKGELINSLGHIYRIVGEKGISSLIYNTIVGNQVIIRGSSKSLVESLVKTLSNLIPQECCLIDSYSDTFKELWECNILGIASDVIIPRHIDNDNTAIIDIEYYDRSNEPDDIDHPNRSTIDINETGPSFPTTMGDAIETVLRQNYPSVIESTHLKMIKDEWINKSKAFYGLKTIIENERDKRLYGFLNILNCHHPEKDRDILKFWSSCAKRSFIQMEKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q111</accession>
    <name>A0A8J4Q111_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Complex 1 LYR protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001896</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000048">
        <property type="protein sequence ID" value="KAF2076819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q111"/>
    <dbReference type="OrthoDB" id="16028at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20251">
        <property type="entry name" value="Complex1_LYR_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="1">
        <location>
            <begin position="5"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="125" mass="14751" checksum="ED7704BBC321789F" modified="2022-05-25" version="1">MVNTTLHLYRNLIRASNLFLVSHARKDRCLSTFIKDQTQKSFRENKDVNDSTKIKQLLAKANRDYLNFKDLVENKNLSLYPCSIKVRPDYVQKSNVLLSSANQDRIKNKKWGFVDRLKVIFTREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q114</accession>
    <name>A0A8J4Q114_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein RFT1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_001429</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Intramembrane glycolipid transporter that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. RFT1 could mediate the translocation of the cytosolically oriented intermediate DolPP-GlcNAc2Man5, produced by ALG11, into the ER lumen where dolichol-linked oligosaccharides assembly continues. However, the intramembrane lipid transporter activity could not be confirmed in vitro.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Endoplasmic reticulum membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the RFT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000034">
        <property type="protein sequence ID" value="KAF2077240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q114"/>
    <dbReference type="OrthoDB" id="9979195at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034203">
        <property type="term" value="P:glycolipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007594">
        <property type="entry name" value="RFT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13117">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MULTISPAN TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13117:SF5">
        <property type="entry name" value="PROTEIN RFT1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04506">
        <property type="entry name" value="Rft-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="399"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="433"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="457"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="499"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="527"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2077240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="565" mass="64679" checksum="6ABC16A1E9CAE228" modified="2022-05-25" version="1">MTNLKTKNNEVLEQQALASKSGGVLSKTISGAFYLIALQIISRLFTFVLNILMVTSVDTNILGVYSVQYQLLSSTILFLSRESIRRSCSRIDISSSKSNFQSVVNTSWLIIPIGMILSISIENFFLINTEDSIKGIENYYSGLRLFTYSSMIELASEPLYILYQNLLLFKIRTFVEGFALFIKTITTYYFVVIFNDGLKGFGYSQLFYSLSIFLGYFGYFIYKVLNTNDSNRKKDKEEENEQFQFNSFKQILPNPSKSIDKHLLKLTGLYTWQTIQKLLLTEGEKYILYFSETLVSQAIFSVVSNLGSLIVRFLFLPIEESCFLMFPKLFPSISNSSNNNIIDKKEKEKELESGSKILAVVIKFLMIIALTFICFAPNYSELLMNLLYSSKFQGTNAGVILGVYCVYVGFLSVNGVTEAFVHSVSKENQLKTLNFILIIIGFAYLFFSYVLCKIFNSVGIILANILNMLLRILYSFYFMNQFFKELKDFSLKSMMPHKLVFIIYFTSSIITYLSSKYIYNANSFKSSIIHIFVGLICFGTSLVILYLKEWNSIKEFKKVLANKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q124</accession>
    <name>A0A8J4Q124_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)</fullName>
            <ecNumber evidence="4">5.4.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_001444</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23536"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="5.4.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.2" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000034">
        <property type="protein sequence ID" value="KAF2077255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q124"/>
    <dbReference type="OrthoDB" id="2291at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004614">
        <property type="term" value="F:phosphoglucomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03085">
        <property type="entry name" value="PGM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.50:FF:000002">
        <property type="entry name" value="Phosphoglucomutase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000004">
        <property type="entry name" value="Phosphoglucomutase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000005">
        <property type="entry name" value="Phosphoglucomutase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000040">
        <property type="entry name" value="Phosphoglucomutase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045244">
        <property type="entry name" value="PGM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005737">
        <property type="entry name" value="PRK07564.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22573:SF2">
        <property type="entry name" value="PHOSPHOGLUCOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22573">
        <property type="entry name" value="PHOSPHOHEXOMUTASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24947">
        <property type="entry name" value="PGM1_C_vert_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="10">
        <location>
            <begin position="17"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="11">
        <location>
            <begin position="191"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="12">
        <location>
            <begin position="302"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2077255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="565" mass="62277" checksum="A4C93D4BC2BAB427" modified="2022-05-25" version="1">MTKLEIKTISTKPFEGQKPGTSGLRKRVTEVKDNEYYLHNFVQSIFNVLPKEQLSGATLVVGGDGRYFNREAIQIIFQIAAANGVGHILVGQNGLLSTPAISAIVRARKALGAIILTASHNPGGPKGDFGIKYNMSNGGPAPESVTNAIYKQTTEIKEFAMATNVSVADLGELGKHQWDGFSVEVIDSVDDYLALLKTIFDFNQIRQLVANPKFRFNFDAMSGVTGVYGHRIFSQELGVPVQNLINCVPSEDFNNGHPDPNLTYAPELVKKMKAGEFDMGCASDGDGDRNMILGNKFFLNPSDSVAVIAANYKAIPYFKSGLKGLARSMPTSAAMELVAKDLNVPFFEVPTGWKFFGNLMDAGTLSICGEESFGTGSDHIREKDGIWAVLCWLQILAHHNANSASFVSIEDIVQQHWKHYGRNYYSRYDYEEIDTKPANEMMAHIKEQINTKSLIGKRFTTSANGSVLEIASADDFEYKDPIDKSVSSNQGLRIIFTDGSRIIYRLSGTGSTGATVRVYFDKYEKDHSQLSFDTQSHLSSLINIGLVEIAKLQHYTNRNEPNVIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1D2</accession>
    <name>A0A8J4Q1D2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Deoxyuridine 5'-triphosphate nucleotidohydrolase</fullName>
            <shortName evidence="8">dUTPase</shortName>
            <ecNumber evidence="8">3.6.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">dUTP pyrophosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_001765</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in nucleotide metabolism via production of dUMP, the immediate precursor of thymidine nucleotides, and decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>dUTP + H2O = dUMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10248"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61555"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="3.6.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the dUTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.23" evidence="8"/>
    <dbReference type="EMBL" id="AJWJ01000044">
        <property type="protein sequence ID" value="KAF2076927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1D2"/>
    <dbReference type="OrthoDB" id="10261072at2759"/>
    <dbReference type="UniPathway" id="UPA00610">
        <property type="reaction ID" value="UER00666"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004170">
        <property type="term" value="F:dUTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046081">
        <property type="term" value="P:dUTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.40.10:FF:000004">
        <property type="entry name" value="Deoxyuridine triphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008181">
        <property type="entry name" value="dUTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00576">
        <property type="entry name" value="dut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001862">
        <property type="entry name" value="PRK00601.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11241">
        <property type="entry name" value="DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11241:SF0">
        <property type="entry name" value="DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="dUTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="dUTPase-like" evidence="9">
        <location>
            <begin position="17"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2076927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="149" mass="15875" checksum="81086FE8B778DD74" modified="2022-05-25" version="1">MISIENMVLKVKKLSEKATIPVRASKGAAGYDLSAAADCVVPARGKALIMTDLQVAVPEGTYGRVAPRSGLAWKKFIDTGAGVIDSDYRGNLGVILFNHSDEDFPVVVGDRIAQLILERIVTPEVMEVSSLDDTERGEGGFGSTNVKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q215</accession>
    <name>A0A8J4Q215_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000253</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000004">
        <property type="protein sequence ID" value="KAF2078503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q215"/>
    <dbReference type="OrthoDB" id="10394449at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="487" mass="56074" checksum="9DFF896480D82828" modified="2022-05-25" version="1">MTQLFNLVWRNQYIRHLIRNRVCQNILKRVNKEYLEENHQYLALFSKRDKVENNIRIRFNGDASDYLDINNRDRDVINDIELIIKADFNFNEIHDGVHTLSLKFKGTPAPGMGQLPDSITNLTLIYNDETTIIQHLISNLPCKLIQLSFVNDDCIQSKCILPHTVTDLHYEGRYDSMKWLVVPPKKVYRTCTLELDSVESFQWLLENKFIGKVVIHSGSIPVLKSEKLPSHVTNVDFLYGIREPDLLLPHTVVRLCLNYGTPFSHISHLKSLIIQCDYEIKLEKGVLPQSLEDLSLSYDQPLELDVFPPSLTSLYLASFNQPLNPFVLPQKLKILCLYSFKQPTFFANSLPVSLTVLTLYEFKGSFDHCQPLDNLKRLRIDSLVPSVAGLLTNVKKLDLWVNTKISDPFGTCLVNTSIESLCLNINFKSTLYPNSFPSTIKYLSLDNVVIESDNVIPTSCTYLNFNENFEPKYIPKSVRYLKAYKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2D8</accession>
    <name>A0A8J4Q2D8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000003">
        <property type="protein sequence ID" value="KAF2078546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="22524at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1334" mass="153819" checksum="009FB5C19803C376" modified="2022-05-25" version="1">MCIDIEIHNSISFTKWSFNKESRSNDCEPIYISDVKTREIKWNDTTNIQKYNSVLILYLFKYYQGDYDQTILSLIESLPKQIKVVKVHLYKTSQNPEKIIVRERLERSPQHIGNLLNKIKDVLGEKSETVELVVLKENEMDKQSYSENTKYTFKETNVLKEIPVVDSLVWDTKRRVPQVYQIPYGVKRITFIGINEPIPDVIPVSIRAVKFKQMNQCLSLFSFPYSVQYLSLDENFKQSIYDSRGVLHLPSSLSHLEITVDMDDPYHWLYELSTLPSKITHLKIHSLRETGTFKHILLIPSSVIALDVSDCLVKNIKLIDTDTYYHKNKDQHQHQDQPLSNLKSLKLPKNYKQIITNQSFPVGLETLDISNVQNIKDILDPSDLSFLLPCSLTSLTCSSPDMIPDHVRLNYLEYSIQPYVKKIVFKNTERQITRDKVPHSFIPRTSTLKPLPLLPIEEFETMAYCEDIIPPSPKVNIQQLNTMYVLSIPKSVEKLRITDFNQESFQKLKLPPNLTELETYYLEASVKIPKSLKSIKLLTCTYDPAIIPSSVTQLSIKVDENTISTLCRHNNNSPKYPPNGIIKIFSPHIIKSKSSLQTVLSYRDFKDCKNIGKNNMTAHASSQVPSFLKIWRNQYLQKKIYDYYYPTVITLSERNNENDINFITERFINVNLKLFNEYSVYRDRVPVVSNLDGFIFNAYGPKTHIPITPTIRYLETDYLVKIPDGPTHLKVSKGIKKSSMSGHELIPASVKHLHLAFQFIKYSMVPDTVYHLVIETADIVANAIPPSVQILEIKNSMKNNFTLDQYQDLIPSTVRTLILDEKDLRQEAFHNNSIPPCIANILSQAYHVYDSTRQTPLNTSVLIWPKNELILHGDIPFGVRRVIFGDEFNQFISQNSIPPSVTEINFGKHFDQCLSNIHLPISLKYLSFARFKRDLVKHSFPPLVSHLSINFVDGYPITNSIENLAESITHLDIKCDKLLYVPKFIKHLKVNTRYGGFGLNPFLNSIQSNQIISIYDKEEKESIDQGEQVEDPIPTDINNNNNNNNNCKKKVNIHLEFSSNFTQPILPGTLDHLNIKSITFGEWFNQALPIGSIPESVEKVIFSRESQFNQQLFRFENVTFLDLGFKFNLPLRKFMFPKTLKYLYLSECFNQPIKRNMLPKTLHSLYFGLKFNQPIQRDVLPRYLKVLSFGDEFKQKLSPGTIPNSVEQLSFGVNSILYPRSIPSSVRKLILKNPISTQTLIECVPNSVTELIIQEHKNEWTESVELEAKHLPQSLTILCIDHPKQTIYNIDHLPPTVTSLSLCNHLFKSKIPPSIIDLQLNGNFNKPIKHIIDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2G6</accession>
    <name>A0A8J4Q2G6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000091</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000002">
        <property type="protein sequence ID" value="KAF2078591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2G6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2078591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="148" mass="17064" checksum="B1A669F0003034A5" modified="2022-05-25" version="1">MTDKQKDNDGVFPRLVGQLVNLILNDSNYFEQYPRALVVGEYQSNPKKFITTDNISFDLYDTPVTGQEIGFTLTKFASQKLSSKDDPENYMFEFVLDLVQRQVIDVKHFGAKSGFDKVLYGQSFSQWWAVKDIADHFQAFKTQDQQAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2J1</accession>
    <name>A0A8J4Q2J1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase M28 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001867</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000047">
        <property type="protein sequence ID" value="KAF2076839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2J1"/>
    <dbReference type="OrthoDB" id="2214at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20916">
        <property type="entry name" value="CYSTEINE AND GLYCINE-RICH PROTEIN 2 BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20916:SF18">
        <property type="entry name" value="IPT_TIG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="479"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="601"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="641"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="679"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="3">
        <location>
            <begin position="175"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="419"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="531"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2076839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="718" mass="82266" checksum="653569BE6FA3908A" modified="2022-05-25" version="1">MGIFKNIDYHLIISIFVFVSIFSLSRYIYNTQYNVVNDSNNNILLDANSQFYIYQQQQLRNSLNHVESDFNQKNVNNKKQNPNIFDVDAHQVIRHTENLIDIGNSKLVYEESRSLARDYIKEYLMTSGGIPSDNIIFNRFEWSERRAEGKNITTTTWTGINIMIWSNSSIAAPNKKIRVIGSHYDTVHWGLNRTSGAHDNLSGTGLLIETAIQFYRYNQFLKEQDTTGTYQETPYLFVLFDQEEPGALGSKSFIDYYKIKKNSSKYAYFIDVDAIGYKNALPIIQTYPYEHNHQTLFSPRWLVDRMIQSAYQVTLDGIGVGNPKIGLSLMYQAHRYHLLSISYLSDDGPFTWSGVDSLLLTDLDIFYGKNPDYHKQTDLPNNLDADHLKITSNILSHFLLSTISDYSLPNIDLKQNNNNNQIINNNNNNNNNNNNNNNNISQSLNNQSIWNNPMLRLFNQLFNSLIDLLFSGNKQYLSIGPLCLGFFDLISISLLLLSIIYYTSFKSYREIVLQYEKLRYQKRKRLYRKSKNDASSAMGTDHEESPSASEVEDVDIGMDSNSINNVVITPSPSTIIETNVGVSSFGSSTRSFFDSVRGHRILFVHLLTLAIISLIDTVYSFEIICLGFLALTTIHWHKYNINMITSFISGAFCTNFIYKDISQMISLGRKGTGSQELSLGIFLTIYIIHSILIVIYGYDLGRKKVELIKFHTDKTKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3D9</accession>
    <name>A0A8J4Q3D9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome P450 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_001337</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000031">
        <property type="protein sequence ID" value="KAF2077334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3D9"/>
    <dbReference type="OrthoDB" id="2789670at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00302">
        <property type="entry name" value="cytochrome_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF54">
        <property type="entry name" value="CYP9F3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="464"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2077334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="528" mass="61886" checksum="71E098D7E0F806AE" modified="2022-05-25" version="1">MEFMDIIYFLVLIYLTYVVIIYLVHRKTRFLVHPIKGVMNGVNPVFIFGDIPFNLFGLKPRATNALGDIYYRWFFWNPVVEIKDLNSLHYIYNMNCINYPRSWNQSNSPLNILSKSEAKRFYRIYFNALLSKNTMSELLPIVREEVFDFIKSKIDPPSNRVLSTNIDIPKFLLKLMSRVYLGSGEDCLECLTAFYDKFHNNPKTFLHFLFPLLYKLPTKFSKNYQESKSKRLLFKLIAMKAYYRVQPCSDDDKIEGSLLNVIAKASYNDREGLSLDEIIIPYFILNITSLCSPISIMTSFIYMMIQHPEVEDKIRNEINQVLKRNNHTVENFTYDDMLEMTYLDASFDELVRLYPPFGRLIPRVAKNSDRVLGYHIPKGTTITCPVSDILRDPKYFEEPDKFKPERQFKFNAILDSPPLKYCPGSSHLFSPTRSRESMELAKDDIERNKRIVKSMPWGVGSNKCMAKEFSKMMIKTIIITLYSQFTFRSIKDSEQYKFSNIKIEQIFQPTVEPPLLFKPIPISQSSQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3K7</accession>
    <name>A0A8J4Q3K7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">60S ribosomal protein L27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_000571</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eL27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000011">
        <property type="protein sequence ID" value="KAF2078100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3K7"/>
    <dbReference type="OrthoDB" id="2365484at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06090">
        <property type="entry name" value="KOW_RPL27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001141">
        <property type="entry name" value="Ribosomal_eL27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018262">
        <property type="entry name" value="Ribosomal_eL27_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041991">
        <property type="entry name" value="Ribosomal_eL27_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038655">
        <property type="entry name" value="Ribosomal_eL27_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10497">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01777">
        <property type="entry name" value="Ribosomal_L27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01107">
        <property type="entry name" value="RIBOSOMAL_L27E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2078100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="119" mass="13968" checksum="EE9327565B380AAF" modified="2022-05-25" version="1">MTLYLPFDDANKQRPYGHCLVAGIDKYPRSIVRSMSRKAILKRTAIRPFVKMVNYNHIMPTRYNFESRDESSFNGLKESVTVETIQPAKRTHTRLAVKKIFEAKHKAGKSKWFFSKLRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4B9</accession>
    <name>A0A8J4Q4B9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mediator of RNA polymerase II transcription subunit 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Mediator complex subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MED6</name>
        <name evidence="8" type="ORF">CYY_000089</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the Mediator complex subunit 6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000002">
        <property type="protein sequence ID" value="KAF2078589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4B9"/>
    <dbReference type="OrthoDB" id="344220at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.580">
        <property type="entry name" value="Mediator complex, subunit Med6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007018">
        <property type="entry name" value="Mediator_Med6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038566">
        <property type="entry name" value="Mediator_Med6_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13104">
        <property type="entry name" value="MED-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04934">
        <property type="entry name" value="Med6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="244"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2078589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="254" mass="29166" checksum="3CB0A0D70968873D" modified="2022-05-25" version="1">MMDNQSVSGGTSNSYNETTINKIMEKEEPDLTQVMWRDPLWLQMFPLNAQTVLSYFYYSQFYDKSCNNEILKMQRLELSSLKTMIGIEYELAHFVEPGFYVIAKQNRVSPTDAVIQTLYYVINGNIYQSPYLRSVFTSRISQSLYHLSEAFSNISSTINWDITSGYSMNLDPNKQQEKSKLAAYSRKQIEDTKRLDVLINSLFQKFPIIPKPMVDNQPQQPSPILVPHITGIAPPVQNIASPPQQQPQPQQPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4M4</accession>
    <name>A0A8J4Q4M4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000001">
        <property type="protein sequence ID" value="KAF2078637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4M4"/>
    <dbReference type="OrthoDB" id="16405at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902936">
        <property type="term" value="F:phosphatidylinositol bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174">
        <property type="entry name" value="ALPHA-TOCOPHEROL TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174:SF208">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN DDB_G0278031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="87"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="257" mass="29873" checksum="0B45119F009DC58B" modified="2022-05-25" version="1">MLNITPDIIPDSPKSYRILNGLSQEEQLKLEEFRNEQIAKDIEPQYLMLYLFAKKLIVADAAKLLENNLNLRKKLDIPFPVLKKDVNSEIALKASSFSIIGKRDKLNRSISYLIPHNLVPKDHDFKEYLTSILWNIDQSVNDDSSSHRLGMVIVEDLKNFSLFKHFDKRLSQHVEGKMENVFPGRIQKIYILNPPFYVKPMLSFAKTFVKNKIVKRVEIVKQERLLQDIDSDQLLPHFGGSYGTSYSQYFDSLPHDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q874</accession>
    <name>A0A8J4Q874_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">THUMP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_002189</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000058">
        <property type="protein sequence ID" value="KAF2076511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q874"/>
    <dbReference type="OrthoDB" id="367221at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11717">
        <property type="entry name" value="THUMP_THUMPD1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2300.10:FF:000001">
        <property type="entry name" value="THUMP domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2300.10">
        <property type="entry name" value="THUMP superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040183">
        <property type="entry name" value="THUMPD1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13452">
        <property type="entry name" value="THUMP DOMAIN CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13452:SF10">
        <property type="entry name" value="THUMP DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143437">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="THUMP" evidence="3">
        <location>
            <begin position="158"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="279"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="279"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="298"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2076511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="328" mass="37982" checksum="E5E3AAB6CD260CDE" modified="2022-05-25" version="1">MSNQESQEQDVASLKRKETEEFLELENQIFKKQNTSSSTDLPVPNAKVLSDPIYGCKGLMYTFDSGRERQATKDIHVLMDQYLQPYRNNNSIDNNSSDSSYDFNAEFLKELQQVRDENKRENRLYKTHSLKCNGLGFISFRSDTDIDPIDIVNRICLDVEKDKLVKTKEITRVIPILKTCHVSQITPEIKLLIDKYFKNNNNQIIKYKIEFKSRNNSKIHKMDYIQEIAKTMDPSKHKVDLVNPDLTIIVEIINFFCALSIVSNFEKCKKFNLANLAGLPPNNIPKGKKNNSNNNNNKKSESKEKVEQEEEKEPKTSESTSKKEEIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UP64</accession>
    <name>A0A8J4UP64_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009913</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000851">
        <property type="protein sequence ID" value="KAF2068761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UP64"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="93" mass="10743" checksum="067A7B684565E8D3" modified="2022-05-25" version="1">MVIPTIKATPMMEPKMMPKRGTDVSALDIVWIGYSDTKEIEFILDDEKANILRSWTLETFDYVIVDDLNYEDKAIHETPRAKFKHSIQLKKGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQ36</accession>
    <name>A0A8J4UQ36_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MACPF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_008556</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell outer membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000538">
        <property type="protein sequence ID" value="KAF2070126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="21110at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003368">
        <property type="entry name" value="POMP_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF5">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02415">
        <property type="entry name" value="Chlam_PMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035304825" description="MACPF domain-containing protein" evidence="8">
        <location>
            <begin position="20"/>
            <end position="2226"/>
        </location>
    </feature>
    <feature type="domain" description="MACPF" evidence="9">
        <location>
            <begin position="1453"/>
            <end position="1792"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2070126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="2226" mass="250813" checksum="8AE354BA27D37F5E" modified="2022-05-25" version="1">MKILISLLLLYIFFTAVFTTPSLLLKKRLDPGPSDYSNTGCENSKYYLVLKDLIPLKSITSLDNNVGLSFQIKNTENDSFLVELKLSLNYSNSPISTNIKVINTLNEESIFSLDNLSCLPPQTEILALAPPVLDFFMGNAYMIFNIDIDEQIYRPGLSCLSNFGDCSASQIQPNQIIVFIFVNSTIVSYKALTFKLLNLFSNVVFEIQFDNPLKSDMATYMIEQDGKWSDPNSENNNYLVKVQRNGTMGDFLIFGDTIYSTELFVPVLGNEEKQMGILIVNFPEYKSSASISFYRMSPDPTNSLLKTQTIQKPTLSFPPFVVETSLTLDGVNNVLFFKAQASGMKLNTPTSFTYHSTLKTLPFPYGFTKVANQFLFTPVQMFGRLSSYDIRFFSLHNIGNQKFFKSIQLPSSSPIDNKEPTLVRVLSKKVFEQYISFKVHLADEGSGIFTITNGATTVYALDSLVSGSLNDGVFELVLNKFDYSNRLDLGAYDLYGQYSTFVENVPMDFNLEKIDQIYSDICPSISEIIEFELSSNKIDSSYQERGIYIFFKLSSGDNSMKPMVQITESNGIAEIFVGEFDHSIQKYRIKISVFRKSHLSTIQYILFLNQCLYTSYSLRSKFGETANIKKIRSDVDSYPPMINQVAFINGQSINVSSTIEIGWLISIKDKPSGLQYGNITVVSDVDPYPRDFTFDVSSVNRNKFDSSYEIKFNVSVQHLNQTFKISSVYLEDSLGRKSIYPSGAYKTLSPFYELGVNNYDITIITPKVAEQTPPEITSFSYTTATNNMYTFVVKAQDVGSGININRPPVVFVQSYQALYQNFTTKYVSKIGNTVTFEVVAQIDFKMTFHGCHLSVYGVYDNDLNLSGYSSLDLKNLGYNHFIKTAYIPGAFIKSTSPISQVGGRLTIHGIRFKENSLVYLNNQLVEPLFICSIAIVIDVEPMVDSFEIKISTNTIDSNIFVVIPSLVFHSNILFVDPNSTCTNGCGGFVNPYSTIKAAIHISQSFNTIILKDGVYSGNLNTGLDVFDGKPLQIKSLNGKSKTIIDCEGYSYFLKVLNSLLLSLTEITITNCKSNKGGALYLQETDSILTSVQFVNNQASNGGAIYFFQSKASLKDIYFENNKVLKNGAAIYSYLSDVLVKGDLTVFRNNQNINSKQSRDILCRNSSIEIQDEVPFDKSIFKCMDSCDPSYLHRSLCQDVAQKSIPSKPLKCGDGECNGAESCLSCPNDCSCHYNGLVQEIFNPTCVVTNSSLKSCQSIRNTTLSPPFIENFLGGVDNVVVRLFGYVSVGSSKILPLMLQGTNFGLIFKINGVPKLSFNQNTRFNETVSVFLTSKYAHFIEIILFSKNVDGPQRSFILAPFADPKDRLFYSNLNCGDGILHDEEQNETSRYYCPVDHKYPQYKLEPRCGDGICNEEPNSCYMDCFQELTKTCPARTVPENHLSPGFYYSEDTLGDLISNQFIWRLPGSEHLSFGMDIMTGEEAPSPLFQFDYCQHIANNIIEDPYRGNVYQIPPELSGKAYPQCTYSTSTDSFNSVQEMSNSQESSSKIDFSAEVEASGGFISGGGGIGFSLEKSSKEASKLSTNVNQRIFKTDLLCKTTFLEIDLDRISIHPKLLSDFSNIKDSVEMVGLIKKYGTHFYKKTFMGGKLTHFTITDESEVNTENESEWQESASASLSASVSGPSFSVDASVEASVDKSQSDSSQSQKSESSTTSRILVYGGTPAAFSPASDFSSSPGFTEWASSIDVLPVPIDYQLYPIRHILNQNWVNKYGINIAKTWADAENIFYAMNNIDQTVSATEYYSLIFQWHTPESTFTMKDYPILKIDYEIVEKNILTGELVHKVKTFSTPIVLTYTDCRGNKEKVFFSHKKQNNYDSSICSSEEAFDTDNSLNYKTTYKICDPTFSTNMDGPIRFDFVAPDFFNSATEPNIQIIVPDQYSNLVFINYTNPLKIVSWENSEAILFDNAGIIYSNSKYYGTVAFENKWGWHWNPKYRVASNNNLYEHDPNQPYNDEFNGFKIVDKHLDCTPDVILACKDYIELSFGGYGNNFDGVKTKNPNTKISTIEIPYTGQGTKQGTYWFNIHNDMPPGDVPLDVFYIENLSHIGMHTRINWIQMYMPRKINEWKFDVSAYHLKSYQNKTMIANEWLEINVDPMELYLVNGLTPHKFYPLTPLASSEPKQLFFYPNYNFRARDQQKLNPSIVYPYYLNLCPDKNPQFYEDGEIFSYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4URR9</accession>
    <name>A0A8J4URR9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006048</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000261">
        <property type="protein sequence ID" value="KAF2072646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4URR9"/>
    <dbReference type="OrthoDB" id="289228at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF122">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN SKYWALKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="219"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="6"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="385" mass="44199" checksum="6F923A3E0964A18C" modified="2022-05-25" version="1">MVQCYMSNTREDLEKDNERLQKENWELRNNFKVGFSNSDLNSFYAQLKTSNNENDRLKQENKDIKHQLEKLSEENDNHNNKMDLLLNKIESLANLNDKLKTEIENVNQENKKNVFQIENYSKMTDLMWEKIESLNIKIENFNQQLNQQRTKQTDLKNQQHYSMEKKVSLTKPNESLNQQLIQQNNSLNSKLNEQNLGKIDKVSILANISYVFIYPLNSKIIECSSFKIINDWIDDSKTMGFELLYEALENKFSPSSFHSACDGKGATITLIKTTDGCVFGGYNSQSWNSDIVYYGDNKCFIFTMVNIHGIKPTKYGPGAKCTNYVRCSLDSGPIFGSGNDIAVGTKEGSYQSFPWTYADTTGKGKSTLTPTKSFTIKTIEIYKCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UTH5</accession>
    <name>A0A8J4UTH5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003894</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000129">
        <property type="protein sequence ID" value="KAF2074791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UTH5"/>
    <dbReference type="OrthoDB" id="21433at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12374">
        <property type="entry name" value="RRM_UHM_SPF45_PUF60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040052">
        <property type="entry name" value="RBM17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13288:SF8">
        <property type="entry name" value="SPLICING FACTOR 45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13288">
        <property type="entry name" value="SPLICING FACTOR 45 SPF45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="52"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="366" mass="42701" checksum="2072CF2022376A29" modified="2022-05-25" version="1">MSGREKVFLGLYENLPIEDDKSVSENTDQLKTDTATINTNKGGEDVRIEGIPQQPSSNSTNTNTTTPTIPKPSLPQTSTTTTPTLTPQLRIPPKLLAQQALKSKQSNTSTTASTQQSNKEQLINIRQKQFEQLQQQKKKQEDENKLQQQQQQQQQQQQVIQNQIPINHNDTLFSEFNNEEEIIDEYNPFHPNDYEKIAKEREKMEMEIKLKRAQELKQRYNFDDERDISNSNSIRKQDIDRGLKNKRTSVFDNNDDNENDVDIDDQSQYSNTPTKILLLLNMANRNQTEYLEEDVYKECIKFGVVNQVIIHSITNSDHVRVFVYFNTTDSSRNALNEFGSRYFDKRKVIPHFYKEHLFFKNKLDPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UTN6</accession>
    <name>A0A8J4UTN6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">CYY_003821</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000125">
        <property type="protein sequence ID" value="KAF2074866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UTN6"/>
    <dbReference type="OrthoDB" id="2914378at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01093">
        <property type="entry name" value="CRIB_PAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06614">
        <property type="entry name" value="STKc_PAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001063">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.810.10:FF:000028">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000802">
        <property type="entry name" value="Serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033923">
        <property type="entry name" value="PAK_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF22">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SAMKA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRIB" evidence="18">
        <location>
            <begin position="307"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="492"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="361"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="42"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="57"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="112"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="124"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="175"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="224"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF2074866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="774" mass="84993" checksum="A1729B40E7069641" modified="2022-05-25" version="1">MDQSKRVSIMREKFEAQTISEAESSPPPTRRPPQPIRRPNNGPATTQPSPNSNTLAPQQQPQPSLSSSGNSLPKRPLPSPAGLKKAPPLPTQPRPALNSSAEDNSSYAGSAPAPPIVVPPRSPNPNMNGHMNGNSSNSHSNHNNSNTVRKISSPININTNPPSPPSAPPHSPLMNGNHSNSNNNNTIPSPPPPPMSKQPKKLAPNAVPAGLGNILAGPPPVIKTPIISPGSTSPSLNSQPTQPTNIPPSSQQSSTIPIATSPTSTPISPPSPPSTHHQQTPQKDEEKKGFLAIFSSKKKNKKKEFSVGSPFNVKHNIHVDYNSVTGFEGLPREWEVILQSSGITKEDVVENSDAVLDVLDFHMNQQKQQQKQEQLEKLQAQAAKLPPKQLPPRPQVDNQAAAQQQAAAQQRLQQQQQAAAQQQAAQQQQLLQQQQAAQQQQMLQEQQLLEMQFTESMCGDEDLEFNNATLPEESNVSLYELISQEDPTKIYGEGSTKIGEGAAGEVFVVTKLKTNQKVAIKKMPLNQQNMKLVITEIGIMKSCKHPNIVEYIDSYLLNDSLWVAMEYMGGGCLTEILEQFNTVKLVESQIAYICLETLKGLSYVHSQHRIHRDIKSDNILLGSDGSVKLADFGYAAQLTKSKPKRITIVGTPYWMAPELIRGQNYDRKVDIWSLGIMAMEMAESEPPYMSFPPLRALFLITTKGIPELKEQAKWSDEFKDFLKKCLEKDVEARPDARQLLSHPFLKQACNSNGLVPSIFESKKAKEAQSKFSIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UUI5</accession>
    <name>A0A8J4UUI5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010037</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000895">
        <property type="protein sequence ID" value="KAF2068638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UUI5"/>
    <dbReference type="OrthoDB" id="20742at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071339">
        <property type="term" value="C:MLL1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044545">
        <property type="term" value="C:NSL complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002151">
        <property type="term" value="F:G-quadruplex RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22687">
        <property type="entry name" value="FHA_MCRS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037912">
        <property type="entry name" value="MCRS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025999">
        <property type="entry name" value="MCRS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13233">
        <property type="entry name" value="MICROSPHERULE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13233:SF0">
        <property type="entry name" value="MICROSPHERULE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13325">
        <property type="entry name" value="MCRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="596"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="433"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="713"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="79"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="437"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="461"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="714"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="755"/>
            <end position="765"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="765" mass="86641" checksum="F1210862C94512BB" modified="2022-05-25" version="1">MDNSSSSSNNDLESSNTPQRPRRTRLESPRAKRAAAVAAAVTAPKPIPKTKATRVSKTTTDYTDDLISSPPSVVAGMDTTTTTTTTATTTAIPVPPLQPAHQRSKKGAPKKEPGEKKTPRKEKAAAATAELEDNLREIVGHSELPFEFESQLDRELQKSQLKNRADLDLYEEQLHQQQYLSSQVFTQDLLQKYLHSHGYGGESTTPSTHNTPQLSSTQLFDTDSNNNNVNTTPLMSTPGVKKPRILKSEHPKAKSPKTSKKQEKLQRKYIRYHTPDLSIRGDWTVHDDQVLLENIQFAASFSEIAQSKEFTKPFTAQDIEKRWRTILFDMNGSDEASGMLALHPPMPKVYPMTKKEESLFTNIPINLTRANFMHLLLAHREFFHHSRTSSSLQSYFKDILLSQDISKFPNYLNFIKDQREKIDLINSLNISGNSIRRRGDNNDNDNKENKENVDSGNDTSVEDKMEIEPNDHTHISKEKEKEILNNEHLIQFLYQKEPTGFLPPYMGGLPHYNSHSIWDNILSTPLQSKSELEDDLILNPFSGIPHFMMIPKDEKRDLKSMIKVEKEYEIDRDKDFKSLAIIRGKNIRYLMKSKDIIVGRNFSDKNRIDLDLNEENPNHINRISKKQLVIKLKSDTNFYIQNIGKNSVFVNGCPLPQYEKIHLQDLSLIEFSDIKLIFEINKSFLAKIKRQLSKSSSSSSYVASSNLGGFGLLTPSTTTSTTTTTTTTSSTSTTMNNNNNNNNNDISTNSMMDHGEEDEDDGENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UUY6</accession>
    <name>A0A8J4UUY6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000074">
        <property type="protein sequence ID" value="KAF2076066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UUY6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="100" mass="10570" checksum="0E51738B13A43142" modified="2022-05-25" version="1">MKAEEILVENVVIVNSCHIIAPPAENIIKDVGVGIENPKSDNSGIESPDNIQECIDISSISSNESPFKQTVHSSNGSPAIDNNNLKSGIVSIDSSHSLCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWS7</accession>
    <name>A0A8J4UWS7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribulokinase/uridine kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008632</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000551">
        <property type="protein sequence ID" value="KAF2070050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWS7"/>
    <dbReference type="OrthoDB" id="6362633at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="1">
        <location>
            <begin position="32"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="217" mass="24646" checksum="2D17CC5A0C194918" modified="2022-05-25" version="1">MTEDQNIQEIIEKLYQSLINNKYDGEKRVLYGLAGAPASGKSTLSSRLVQYINEKAGKDIALVVPMDGFHLDNCILDKRGVRAVKGSPQTFDCAGFVSLLKRLVEDNEDIVYIPTFDRHLDLSRNCASVVTKQHRILIVEGNYLLLNQDPWTLLKPLFNATVFLQVEMDELKKRLIQRWLTHGHDQEAAEKRALSNDIPNAELVLTKSNTSDYLFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UX61</accession>
    <name>A0A8J4UX61_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carbohydrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000431</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000008">
        <property type="protein sequence ID" value="KAF2078241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UX61"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019028">
        <property type="entry name" value="CBM_49"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052879">
        <property type="entry name" value="Dd_Spore_Germination_Stalk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33239">
        <property type="entry name" value="CELLULOSE-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09478">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01063">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035280070" description="Carbohydrate binding domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate binding" evidence="2">
        <location>
            <begin position="35"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="128" mass="14385" checksum="5048F49C13A8E924" modified="2022-05-25" version="1">MKFIFIVFACFLIVLANADLKGECSIGNIGIHSEVVNSYTIVGEGDQVYSNVNVIIHNYSGKRVNYFAIRTGYLELRDDSSIWNIVHVGNDLILPQGQIIEPGNNFTFGFIIKGHFIPPMYVRRVTCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYI1</accession>
    <name>A0A8J4UYI1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL6 alpha-beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_005884</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000246">
        <property type="protein sequence ID" value="KAF2072810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYI1"/>
    <dbReference type="OrthoDB" id="10252633at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000003">
        <property type="entry name" value="60S ribosomal protein L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000004">
        <property type="entry name" value="60S ribosomal protein L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="entry name" value="Ribosomal protein L6, alpha-beta domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_uL6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_uL6-like_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_uL6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002359">
        <property type="entry name" value="Ribosomal_uL6_CS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF16">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="60S/50S RIBOSOMAL PROTEIN L6/L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="Ribosomal protein L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00700">
        <property type="entry name" value="RIBOSOMAL_L6_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="4">
        <location>
            <begin position="12"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="4">
        <location>
            <begin position="108"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2072810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="189" mass="21169" checksum="F608B8F94A0B1E49" modified="2022-05-25" version="1">MKVIKSSKVVKIPEGVTVDVKGRSVKVTGPRGTLKKEFNHLSVDINLVPAKKEIIIDLWFGNRKQIAGIKTVASHIENMITGVTKGFEYRMRFVYAHFPINVACVDGGKIVEIRNFFGEKIVRRIELLDGITCSRNEKAKDEIVLVGNDLEMLSQSCANIQLRSAIKYKDVRKFLDGIYVSSKSVIETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYL5</accession>
    <name>A0A8J4UYL5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Defective in cullin neddylation protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_005605</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000228">
        <property type="protein sequence ID" value="KAF2073080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYL5"/>
    <dbReference type="OrthoDB" id="286637at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032182">
        <property type="term" value="F:ubiquitin-like protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000030">
        <property type="entry name" value="DCN1-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.200:FF:000002">
        <property type="entry name" value="DCN1-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.200">
        <property type="entry name" value="Cullin, PONY binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014764">
        <property type="entry name" value="DCN-prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042460">
        <property type="entry name" value="DCN1-like_PONY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005176">
        <property type="entry name" value="PONY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12281:SF12">
        <property type="entry name" value="DEFECTIVE IN CULLIN NEDDYLATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12281">
        <property type="entry name" value="RP42 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03556">
        <property type="entry name" value="Cullin_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51229">
        <property type="entry name" value="DCUN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="DCUN1" evidence="4">
        <location>
            <begin position="90"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="45"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU410713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2073080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="286" mass="32400" checksum="658DB3EBC8FCEE47" modified="2022-05-25" version="1">MGNHPSKKEKSSNGKKKLDDHKPVVSSTPSTVNKVHVATPSTSETKPYTPATQQQTQPTNTSAPSTTNKVDSSKSNTSVNSNQNSNKPEDKAKRLEEFFEKYKDPEDPTTIGPDGIVKLCVDLEVDPEDVVVLVLAWHLNAKQMGFFSKTEFISGLTKLGIDSLPKLQSHLPKFKKDLEDQNSFKDIYRFAFIFAKENENNKILDLESACNMLSLVIGDRYPHTERLKEFLLTQKSYRVLNMDQWLSILEFSKVINADVSNYDENGAWPVILDEFVEWTREKLKAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYT5</accession>
    <name>A0A8J4UYT5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit</fullName>
            <shortName evidence="8">Oligosaccharyl transferase 48 kDa subunit</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_006687</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the DDOST 48 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000319">
        <property type="protein sequence ID" value="KAF2071998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYT5"/>
    <dbReference type="OrthoDB" id="29105at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005013">
        <property type="entry name" value="DDOST_48_kDa_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055459">
        <property type="entry name" value="OST48_MD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055457">
        <property type="entry name" value="OST48_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10830">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE 48 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10830:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE 48 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23358">
        <property type="entry name" value="OST48_MD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03345">
        <property type="entry name" value="OST48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035339675" description="Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit" evidence="8">
        <location>
            <begin position="21"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="393"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="OST48 N-terminal" evidence="9">
        <location>
            <begin position="26"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="OST48 middle" evidence="10">
        <location>
            <begin position="283"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2071998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="423" mass="47238" checksum="D3C1D89484C8A224" modified="2022-05-25" version="1">MKSFIISLIIACILVSNVFGAVGGKRVLIIVDDLSMVKSHSQFFSTLEGKGYKIEYHSAGDKVELEKYGDFFYDHLILFAPTSDSLSITSSDVLKFIDGGNNVFIAASNGVISDTVRDIATECGIEIEDDKTSVFDHFNYDKQQSEQNLIVADQYIQDAPIILQGLQTPVLFKGVGHSIRQNPLNYAVLTGSSTAYSRKASGVATKLMGKKVGLVSSLQARNNARVTFAGSIDMFSNKFFTSKLGKDTVGNREFAESLLSWTLQERGILRSSNLSIKKISTESNSTVAPEILTIKDEIEYSITVEEFVKDQWVPYVGQLELEIIMLDPYIRTFIKGNKDGEYKSIIKLPDVYGVFTFEATIQKPGYGNLDTFVRQPIRPFRHDSYERFIPSAFPYYAACFSMIVGVFVFSIVFLFHQEPKKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYU5</accession>
    <name>A0A8J4UYU5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Arf-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_006633</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000315">
        <property type="protein sequence ID" value="KAF2072043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYU5"/>
    <dbReference type="OrthoDB" id="21058at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048205">
        <property type="term" value="P:COPI coating of Golgi vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08830">
        <property type="entry name" value="ArfGap_ArfGap1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000014">
        <property type="entry name" value="ADP-ribosylation factor GTPase-activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45686:SF4">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE ACTIVATING PROTEIN 3, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45686">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE ACTIVATING PROTEIN 3, ISOFORM H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="7">
        <location>
            <begin position="15"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="129"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="462"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="129"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="140"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="184"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="223"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="470"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="546"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="573"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="583"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="594"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2072043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="623" mass="67646" checksum="E07855C4E91CEEFC" modified="2022-05-25" version="1">MSLSASASSNIARAKEVFKKLKDEDPSNKQCFECSSANPQWASVTYGIFICLECSGRHRGLGVHLSFVRSLTMDQWSEKQLEMMSVGGNLKAKEFFKKHGVPDSPIQGRYNTKGAVLYKEKISALAESKVWKEPEESSTKKPSSSSSSSATTTSSSTSSLSSSSSSSSSKPRRSNGFDDWDNWESNSSSTTTTSSSSLKTSKESTPKVKKSSFNSDWKESDNNDSYNGNNSSRGSNSYSSSSNNNNSSRSYSSESYNSGGGYNSSNSNGGYNSNSNSNNSNNNSSSRSYSSDSYNSNSNSNNSNKSSSAKSYSYNSSGSYNNSNTNNNSGDGERLKQFSNARSISSSDYYGDSNDSYNGGGGGGGYNSGYRGNGNNNNNGNNQYLDYGLSLLGEGFSKLSTVASAVNHRLQDGTLLSEASTMVSSYIQKAKDALDGNEGGSYNGNNRVSSAEKDAYYNQWSGTNSSGYKNSPFNSRPTNPNNNSNSYNSNNNSYNNNSYNSNNSNNNSNNSSPTLASKNGANKKVSEEEFVGWDVKNDDEEEEESYSSSKKNQKSSSSSSSSKQQSSRKQPQSEDEEEEEDSWVWKDEPKEQPKSTPKKTQSVSTSTPTQNKKKISDWDEWDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UYY8</accession>
    <name>A0A8J4UYY8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">A20-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_004919</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000183">
        <property type="protein sequence ID" value="KAF2073785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UYY8"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050652">
        <property type="entry name" value="AN1_A20_ZnFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002653">
        <property type="entry name" value="Znf_A20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10634:SF157">
        <property type="entry name" value="A20-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10634">
        <property type="entry name" value="AN1-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01754">
        <property type="entry name" value="zf-A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00259">
        <property type="entry name" value="ZnF_A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51036">
        <property type="entry name" value="ZF_A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="A20-type" evidence="5">
        <location>
            <begin position="9"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="43"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="112"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2073785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="246" mass="26483" checksum="CFC0D62417C3FD25" modified="2022-05-25" version="1">MNSEGNQNEQQPLLCKMNCGFYGNYKTNGLCSSCFQKELKKNNNSLDSNSNSSITATTQPIEINNNSNNEDSPSKSKKRSYESYFDITSSPSKFTPPKNPSSPSKQLKQEESNSLSSSPSTTSPAATNTTTTSTTTTTTSSSIPIPTITNTKESDSSINGISPSSSPSIKDLSSSPSKEAFKCKYPGCGKSIGSIMASMSNKCRCGEFFCGKHIHNHNCTFDYKSLAKSNIAKANPQILANKIVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZ33</accession>
    <name>A0A8J4UZ33_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABC1 atypical kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_004725</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000172">
        <property type="protein sequence ID" value="KAF2073980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZ33"/>
    <dbReference type="OrthoDB" id="427480at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13969">
        <property type="entry name" value="ADCK1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045307">
        <property type="entry name" value="ADCK1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051130">
        <property type="entry name" value="Mito_struct-func_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173:SF28">
        <property type="entry name" value="AARF DOMAIN CONTAINING KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173">
        <property type="entry name" value="ABC1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="3">
        <location>
            <begin position="277"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="100"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="106"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2073980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="686" mass="79624" checksum="5EAB81ABD443FEF7" modified="2022-05-25" version="1">MNNLLFKSSTSPVLFSLKNNIKSLFKSTTISNSSNNGFKNIPKFIINNINNNSNNNKYLFNNSNGNSVRSNNIKNESQSLFSKFDNLNFQSKRYYSKQNKNYDNTSKQQQQQQQQSKSQQQKQEQEQIAMQKQHHYIKKSLERIYKYLTFKDLMRIIYNVLMNNRKKVIAIVGLSSSLIFFYYKPETFIGRYIQMIIRFSRAMDTALHVMYYYKVLSQTPELDPTYKGKATETHQISADKILELCLKNGGLYIKAGQYIASLNHILPVQYTKTLAVLQDKAPWRSYEEVEQVFKKDLGLLPSDIFSDFEKIPIAAASLAQVHRAMTKEGDEVAVKVQYPDLQRNFEGDTFTHGVLLSLINLGFPDFEFNWMADEMKIVLVKELDFTQEADNSIRTKEELKNNVYAYIPQVYQQYSSKRILTTEFIHGCKVNDLKKIREWGLKEKDVAQRLMEIFSEQIFIHGFIHVDPHQGNILLSKHPNLPGQPLIVILDHGLYRELDEEFRLDFCNLYKNLVLLNNEKVKKYSLKLGVTNWKLFSSMILMRNFEGASVGLGQSIPKEELERLVSGALEKMKELNVLMKTMPRHLLLILRNNNLLRSINMELGAPVNRFSIMARYAVKGLNSKVDKKTNKVIQLISRVKEKVHLEVLLKGYEIYYSLTAMLVRILLRLGLINPEKEIKKAMEKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZ44</accession>
    <name>A0A8J4UZ44_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7743 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004676</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000169">
        <property type="protein sequence ID" value="KAF2074025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZ44"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF29">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035156654" description="DUF7743 domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="2">
        <location>
            <begin position="430"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="630" mass="71287" checksum="CC01479466650956" modified="2022-05-25" version="1" fragment="single">MYATTLSNFALLAVIVVSFLVSCQGQIHMTDLTDPSAFNKYSPPSLSSKCSFFYTLLFSQDIDDGVIIVEPLSPIISTELISQSTNKYVYLVKVTLAIGESSFDVKASSSSMSVINTISYVCEAPPTTLDIQFFNDTLYDSYDLATSFPILMFKVNNFFRFGSLLTATISTSSFKTPQVSFVSENIYKIWLEYTDDFTQWAYLSDDFNVSVTYSENPSWAPASIQTIKSNYKSNTVESVEYSIYGIDSNNFTKNAHVHQIVEFTATQNIPSLWYFKANLSYLIRARGLICMGNYTNGVVMQMITYDGPQQAVNLNFIYNNNTPSIIDTIITFNYQKPTYIISATSTSLNNGILSSSVSTNEQILSLSVNINSYSYLYSSDGIYPYGILLRTSDLYTRIDEFYASPHSYGLRQFQYGAITTSFDFGTSGLFDKTPPNIESIEQFKYGNIIVYRVHITDDKSGFYEIAGIGNYVSLVSGTLLDGIYEFKSDLENNYYLYPNDIRICDAALNCKSFDIPTFKATDQSLFKFNEFKSFSEINRIYWKYNDIDVSQQGLDNILYICCSKNITYQPHILPVSAIQTATNVNTILGIYNNSNGCYEFNVQIQKNYITGSFNAFYLWLDYQLVIYSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZN4</accession>
    <name>A0A8J4UZN4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UDP-N-acetylmuramate--L-alanine ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010588</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001219">
        <property type="protein sequence ID" value="KAF2068087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZN4"/>
    <dbReference type="OrthoDB" id="2017219at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016881">
        <property type="term" value="F:acid-amino acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="entry name" value="Mur ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050061">
        <property type="entry name" value="MurCDEF_pg_biosynth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43445:SF3">
        <property type="entry name" value="UDP-N-ACETYLMURAMATE--L-ALANINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43445">
        <property type="entry name" value="UDP-N-ACETYLMURAMATE--L-ALANINE LIGASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="MurD-like peptide ligases, peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur ligase central" evidence="2">
        <location>
            <begin position="23"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Mur ligase C-terminal" evidence="1">
        <location>
            <begin position="182"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="330" mass="38046" checksum="70B2175E429F17B8" modified="2022-05-25" version="1">MGHLLSGVSPTSYLIGNGTGFYENNSKYFVFEACEYKRHFLAYEPDYAIMTNIDFDHPDYYLDINDVFQAFQSLTHNVKKGIIAYGDDQYLCKLDNNNNNINIYYYGIGSENDFQARNIQLDANGSKFDVYCFNKFFGHFQLAIFGFHNILNHLAVISFAFLENIGFNQIINQLNTFKGVTRRFNEKTINNIKIIDDYAHHPTEIKATLLSIRQKYPTLNIISIFQPHTFSRTKTFLNEFANSLDFSNKVYLCDIFGSREYNKSTDGGIIIDELMLKIKTHAGASILTLDNIDILFDNNDNRDCVFVFMGAGDIQKFQKAFEQLCIKRVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZU6</accession>
    <name>A0A8J4UZU6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_005129</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000197">
        <property type="protein sequence ID" value="KAF2073543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZU6"/>
    <dbReference type="OrthoDB" id="4187154at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="6">
        <location>
            <begin position="348"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="350"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="128"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="164"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2073543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="433" mass="51300" checksum="8633653306EA449D" modified="2022-05-25" version="1">MDNNNNRNIFNNNNNNSNNNNNKMNPHVQQQFTSQNYYHILPPEYYTFFIPQPHFHLQQENIHQSTTLAIHKPVSPFDRSTNTNEQFKGSIPVIEILDNDSEGLDIADIESIQENYTPRINSIPMTYNQHQHQLQDQDDDDIEDNYNYDQDEENQDDNFNYYYNDDEDQDDQEEEAQDQEILSKSYLEFIQVKEISIKKKIQKLRDFNVKIQRDVDKVLHQIFTQFSSSLVLKGAKDYSHLKYKTLDPNLNTYIQQEKIDTHSSKKNLDILFPLYNREISNIERMMKEFIYCSSVVHDLFSLIRPCHKTELEASINHIQGYFILMMAHQKSFACGFLYKLKTIFGQPKGSPKSRRTLNDEYKSFINDYFDTNSTDPYPNDDEKAIISGICNISKYQRNNWFSNKRSREKNQRIACLNNKNNNNSLHNLNKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZX3</accession>
    <name>A0A8J4UZX3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">KRR1 small subunit processome component</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">KRR-R motif-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_003758</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the ribosomal small subunit (SSU) processome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
            <location evidence="1 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the KRR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000122">
        <property type="protein sequence ID" value="KAF2074925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZX3"/>
    <dbReference type="OrthoDB" id="441223at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22393">
        <property type="entry name" value="KH-I_KRR1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22394">
        <property type="entry name" value="KH-I_KRR1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000011">
        <property type="entry name" value="KRR1 small subunit processome component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000014">
        <property type="entry name" value="KRR1 small subunit processome component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041174">
        <property type="entry name" value="KRR1-like_KH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048550">
        <property type="entry name" value="KRR1-like_KH1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048548">
        <property type="entry name" value="KRR1-like_KH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048549">
        <property type="entry name" value="KRR1-like_KH2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024166">
        <property type="entry name" value="rRNA_assembly_KRR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12581">
        <property type="entry name" value="HIV-1 REV BINDING PROTEIN 2, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12581:SF0">
        <property type="entry name" value="KRR1 SMALL SUBUNIT PROCESSOME COMPONENT HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17903">
        <property type="entry name" value="KH_KRR1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21800">
        <property type="entry name" value="KH_KRR1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006515">
        <property type="entry name" value="KRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="KRR1 small subunit processome component first KH" evidence="10">
        <location>
            <begin position="60"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="KRR1 small subunit processome component second KH" evidence="11">
        <location>
            <begin position="143"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="255"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="296"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="255"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="296"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="334"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2074925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="383" mass="43926" checksum="F7416E78FDA580EA" modified="2022-05-25" version="1">MSEDKSTTTTASAAGEEKKSYSYVKKKDREDLAIPQGIDPWKAMPLAREDVPRRVLYDDSSFATLFPKYREKYLKEMWPLVESELSDHGITCKLDLIEGSMTVSTTKKCWDPVAILKARDLIKLLSRSVPFQHAQKIMKDDFNCDIIKIGGFVRNKERFVKRRQRLVGPDGSTLKAIELLTKCYVLVQGNTVSSIGPWNGLVAVRKIVEDCMKNIHPIYNIKELMIKKELEKDESLKNESWERFLPQFKKKNINKKKAKIEKKKKDKDAAPFAPAQLPRKEDLDMESGKYFLSEEKKQKKIKAEREAKHAESEAKRQTERQKYQVAPAEKQRPTTTDTTTTTTSSKSIQDSLNNIKNNLKTNNKRKETSSSGADFVLTKKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZY1</accession>
    <name>A0A8J4UZY1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010213</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000976">
        <property type="protein sequence ID" value="KAF2068462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZY1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="90" mass="9956" checksum="045EA53269E5BD07" modified="2022-05-25" version="1">MSNSFSDSLQILKLDNKTLECFTAFEIFKCATPLGIVPLMSTNCNLYRGEQSQCVKVSGRSRLRESTYTVPSGTGKSSTPSAIYLMRVYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V058</accession>
    <name>A0A8J4V058_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_009900</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000847">
        <property type="protein sequence ID" value="KAF2068777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V058"/>
    <dbReference type="OrthoDB" id="19313at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097039">
        <property type="term" value="P:protein linear polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23820">
        <property type="entry name" value="RWD_RNF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051628">
        <property type="entry name" value="LUBAC_E3_Ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770:SF13">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770">
        <property type="entry name" value="UBIQUITIN CONJUGATING ENZYME 7 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RWD" evidence="8">
        <location>
            <begin position="13"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="197"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2068777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="540" mass="62471" checksum="0D42D2FAFBCEFFD2" modified="2022-05-25" version="1">MTDIDYCKEIQNQEIQVISNIFADRFTTLYNYEGISWKYNIKINVDVPQNFSIATSNNIDGKDESSYTLYPIKNLPYIELGFEYPLEYPEKPPIISLASVWLSLEQMYSVVKKLESEWSIGEMVIFKYYDWIKTEIFQYLNIEKIQIFTNEPVDLESPLCSTVSWQYQQSWDSLISTIPFLISFCKQEQKKQFYLEISFECPICCCDYSPQEMVLLDCLHFSCRDCTTSMCKINIESGSVKLLKCSHIGCEQLLDQQFIKSIVSDQEFSQYKTFLQQSKGFVRCKKCDGWGYIDNLTRNCYCTLCKYCWCTLCHEKVHYGLPCYYVAVPKDQINPKQNSFWSDRKVQPVIQEVKVIITCKKCPGCGALLDKFDGCNKISCPNCKLVLCWICLSNINGYDHFQDNSQCILFTSTLPQMEKVPIFPSPPPLILDKEKGNPKDISFCGKCKNLIFRSNNNNHSLCKYCNSSVCFLCKEFIKGTLHFSSSDCVQHGDPPNKNSDIDQAIVSSQISRINNPKRKKNEKIIEPINIKEVVYYDTSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0V2</accession>
    <name>A0A8J4V0V2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DEAD/DEAH box helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_008974</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000610">
        <property type="protein sequence ID" value="KAF2069707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0V2"/>
    <dbReference type="OrthoDB" id="64767at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18024">
        <property type="entry name" value="DEXHc_Mtr4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.30:FF:000003">
        <property type="entry name" value="ATP dependent RNA helicase (Dob1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000083">
        <property type="entry name" value="ATP-dependent RNA helicase DOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000141">
        <property type="entry name" value="ATP-dependent RNA helicase DOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.300:FF:000001">
        <property type="entry name" value="Mtr4 exosome RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025696">
        <property type="entry name" value="Beta-barrel_MTR4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048392">
        <property type="entry name" value="MTR4-like_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050699">
        <property type="entry name" value="RNA-DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016438">
        <property type="entry name" value="SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012961">
        <property type="entry name" value="Ski2/MTR4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131">
        <property type="entry name" value="ATP-DEPENDENT RNA AND DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131:SF7">
        <property type="entry name" value="EXOSOME RNA HELICASE MTR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08148">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21408">
        <property type="entry name" value="MTR4-like_stalk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13234">
        <property type="entry name" value="MTR4_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005198">
        <property type="entry name" value="Antiviral_helicase_SKI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01142">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="187"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="406"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="98"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="825"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="9"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="61"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2069707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1088" mass="122622" checksum="3F944A1F158B6BC4" modified="2022-05-25" version="1">MEDDLFSVFEEKKPDGKNKRKSIVNDEKENGKWIKPKEETTTPQAPAVNDIRNAPLIDQGELDREIQKSKKTKVDLTPVEADISIDSKKEDETLKALREKVEKENSTTTSTSNTEEEQQQESTTNTIQYTATGGTITAAGTAKSCIHEVLLPPGVENNDPDLINPPEPVKPARVYPFKVDPFQAISIACIERKESVLVSAHTSAGKTVVAEYSIAVALRDGQRVIYTSPIKALSNQKYRDLQEQFQDVGLMTGDITINPNASCLVMTTEILRSMLYRGSETMREVAWVIFDEIHYLRDKERGVVWEETIILLPDSIKFVFLSATIPNAREFAAWIAKIHKQPCHVVYTEYRPIPLQHYIFPQGGEGLHLVVDENGNFREENFLKSLSNLQQPEMGNRKKGTKGPSDCYKIVKMIMERSYQPVIIFSFSKKECELYALQMSKLDFNNDDEKKAIETIFNNAIDSLSELDKQLPAVLNILPLLKRGIGIHHAGLLPILKEIIEILFQYGYIKALFATETFSIGLNMPAKTVIFTSVRKFDGEGMRWISGGEYIQMSGRAGRRGLDERGIVILMVDEKMEPAVAKGMVKGQADRLTSSFWIGYSMLLNMLRVEDIDPEHLLKRSFHQYQQEAFIPQLVAKCDSLEEDMKQLFIRDESNVIEYSSIKGQLNKLKEAMRDFINQPSYVQPFLVPGRIVKVRDQNIDWGYGVVLNFYKRPTKSGGVSDKSFEIVADVLLNCEINNNSAAGGVGGNEQQTNPKPCQPGSVGEGQIIPVSIKLFDGITSICLIIKKDQNQSEFKGHLFKVLKETESRFKKDGGIPLIDPIEDMNIKDNNFKKLLRKIESLESRFIGTAGFKENDIEQRVEQLAKKQDIEKEIKSIKKQIRSGDEVILKDDLRSMKRILGRLGYITEDGVVALKGRVACEISAGDELVISELLFMGLFNDLSVEQCVAVFSCFVFQNEGSKDDINNPKIKPDLVPLFKVIKETAQKIVSISQECKLTIDEKQYLSSFNPNFMDVCFSWASGSAFSEIVKMTDTFEGNLIRGIRRLDELIRQMVVASKSIGNTELEAKFSEATVKIKRDIPFAGSLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V229</accession>
    <name>A0A8J4V229_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NADH dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_000812</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000017">
        <property type="protein sequence ID" value="KAF2077850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V229"/>
    <dbReference type="OrthoDB" id="3244603at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045024">
        <property type="entry name" value="NDH-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054585">
        <property type="entry name" value="NDH2-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706:SF13">
        <property type="entry name" value="NADH DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22366">
        <property type="entry name" value="NDH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="6">
        <location>
            <begin position="32"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="External alternative NADH-ubiquinone oxidoreductase-like C-terminal" evidence="7">
        <location>
            <begin position="374"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2077850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="437" mass="48927" checksum="7D2339E997C1B439" modified="2022-05-25" version="1">MLNATKSLFRPLKGSFLNYTGKLGEAKTTKPRIVILGCGWGSYAFLKKLKTHDYEVTVISPRNHFLFTPLLASTSVGTLEFRSIAEPIRNTIKENFNYLQAACTEINPTTNTISCVSTLHKKPFEVQYDKLIIGVGATNNTFGIPGVEKYAHFLKELDQARTIRKKIIECFEFASLPDCSVQERERLLSFVVVGGGPTGVEFAAELNDFFLEDLTRFFPNAPVNEVKITLLEASGKILSAFDEKLVKKALLNFKTSGVNVQTHSGVKDVSNERVLLNDGTEIPYGLLVWSTGNKPIQLINESKLEKDKHGRLLTDGYLRVKSHPNIFAFGDCGQIEGTPLPATAAVAQQQGLYLAKEFNKDPSEVLSPFKFHFLGMLVNIGRHNSLFQTKVIDFSGFFAFLSWRSVYLTRLGSIRAKFQVPFDWTRTLLFGRDISQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V282</accession>
    <name>A0A8J4V282_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Proteasome inhibitor PI31 subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_000683</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the proteasome inhibitor PI31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000013">
        <property type="protein sequence ID" value="KAF2078045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V282"/>
    <dbReference type="OrthoDB" id="68090at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045128">
        <property type="entry name" value="PI31-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013886">
        <property type="entry name" value="PI31_Prot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021625">
        <property type="entry name" value="PI31_Prot_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13266">
        <property type="entry name" value="PROTEASOME INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13266:SF1">
        <property type="entry name" value="PROTEASOME INHIBITOR PI31 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08577">
        <property type="entry name" value="PI31_Prot_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11566">
        <property type="entry name" value="PI31_Prot_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0647">Proteasome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PI31 proteasome regulator N-terminal" evidence="13">
        <location>
            <begin position="20"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="PI31 proteasome regulator C-terminal" evidence="12">
        <location>
            <begin position="207"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="149"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="262"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="161"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="302"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF11566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2078045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="312" mass="34195" checksum="472C3B07681F4CC0" modified="2022-05-25" version="1">MDQYIKDNLKTIITARPDAVRDKFDVTALVLHLVMCKHEFKCEEQDTSSTSTMGLPATWNSNTDSYTFKYKNAKDTSNKTFLIKSLRMGNVLLVNAMSLDKQETIYTLDIDTDKLVKNSNFSDVDNLFMNLTELVTLFEHSIISPLAPTIVPPPQSGGNTGNSNNGTNSNNNRNYYQRPPPQYAPPLAAGGGGNRNDNHHQQQQPWGDDDLYPGGLPHIPNMAFQPSRVGPGGFGGSSVGRDHPGFGNVHYNYGDDRDYGSGLPGFEGRLPRGAVPPGARFDPFGPPKAPGGHNRPDRDDFPPPGFNPPGYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2H4</accession>
    <name>A0A8J4V2H4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CRIB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_002498</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000069">
        <property type="protein sequence ID" value="KAF2076198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2H4"/>
    <dbReference type="OrthoDB" id="21137at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00132">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011026">
        <property type="entry name" value="WAS_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159">
        <property type="entry name" value="CENTROSOMAL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159:SF31">
        <property type="entry name" value="CENTROSOME-ASSOCIATED PROTEIN CEP250 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47912">
        <property type="entry name" value="Wiscott-Aldrich syndrome protein, WASP, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRIB" evidence="7">
        <location>
            <begin position="185"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="37"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="252"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="572"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="608"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="669"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="768"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="348"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="112"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="252"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="269"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="282"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="299"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="572"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="608"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="620"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="632"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="677"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="696"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="774"/>
            <end position="789"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2076198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="789" mass="85901" checksum="58AC3EAD1C3BCE41" modified="2022-05-25" version="1">MTQSCLNCDCKTFDPNPFKPLYCSGCLHIHTKKSPNVSVNTNPPASSGSSNAAKPSLSSSTNNANKSSQVNKQLPPKPVGKSASTNSLLNTSNNSNTSNSNINNKERPKGQNFTKPNTTTTTSSTTKSTASNNSSNSNSTSSPSSSKLSLSSFSNKLGSIKDNLKDKYNTLMSSEKEEQQLGLEISGPTDYKHNTHIGITENGGISTRNIPEEWKSTFQQIDMALKSRGRKGVTKKEATFILNAVLSNQYAKGPASTSTNAPPLPPKPNGNSNNNSGNNSNIASVTQQSNKPLPSAPAKSINISTSTATTTSNKSTYSTPSSYNSNISTSTSSASSYTPSTTKATQVNTIDTKELEQLREKVKQLENKLATSENNLSSITNKYKALESNNQLVVNETKSLENELSTLKKSLLDSKKNIESDKGPLLEKIQQLESKLKDAELEKKSLTAKESELDGRVKQLSNTNKDQEQKLFDSQIKVKELSTQLDSLKKSFELSATANNNASAAEYSKQVQELTRQYSVKEKEIRESYEKQYSSRINQQKTELDSVQASLSKEQSKSSKLELDLASKIRDLDNSTTQKDDLKKQLSSQQTQNEQLLSQLDATNNSLKSLKLSQAQSQNINESNTKQVQDQLKSLSDTNSSLSSKNQELQSTIDKLKAQCSQYEQKMSDLQKSLAESKSNSSSVVSTPPISSGSSTPPPPPPPPAPPAPTFKTAPPAGDSNRMALLDAIKKPDIVLKTVTEDMKTAYDPVEDDPNSMVNLLVKALIDRRAGMNENDDDEDDDDEMDWND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3N7</accession>
    <name>A0A8J4V3N7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Regulator of chromosome condensation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000251">
        <property type="protein sequence ID" value="KAF2072752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3N7"/>
    <dbReference type="OrthoDB" id="70707at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051553">
        <property type="entry name" value="Ran_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982:SF1">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="86"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="212"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="262"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="516" mass="57472" checksum="F2B4B6F9538C19FB" modified="2022-05-25" version="1">MHRVLKTTTTTTLLTLPLQNSCLSSSSSISYQQRGFFNWFGGSKKDENKKRLGDALKEKNKLTSPTVWSENNTKSSSKSSIEYPVTAVYSIGVNHQGQLGLGDCKSECEWVYNEELKSQSIKSIKSSLLHSAAITNDFNIFTWGANFHMQLGQKFLSGLSILPSFMESTPISIKQSSFPNSKVIDCALGGWNGFALLVDNSDNKNSNSKSGQEIFSWGNNQYGQLGTGNLMNLLTPTKIYIPDTIKSISCGLYHTHFLLENGKLLSCGKANDGQLAMSIGNIEKESCSSEDKKHLGITRPRFSDLLGDAKIKEISAGCFHSIALTDDNRVYQWGKGEIKRDSIDDTLYTPYYTINKLDHQINNEINKEKQQQLYKVEEIKFPTDATSQGFKQVIGGNGFSMALTNDGKLYHWKIDGDNSNNPTLVENQSIVGKSISKVSVSPSHIGLIFDDESSRNQIFIWTLDDYKLEKKALKSMFMTTIQDGSKEKLFSLPNDHTIKDLSMGSHFSLIVAEKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3V7</accession>
    <name>A0A8J4V3V7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Carbohydrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008489</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000527">
        <property type="protein sequence ID" value="KAF2070199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3V7"/>
    <dbReference type="OrthoDB" id="19156at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019028">
        <property type="entry name" value="CBM_49"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052879">
        <property type="entry name" value="Dd_Spore_Germination_Stalk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33239">
        <property type="entry name" value="CELLULOSE-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09478">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01063">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carbohydrate binding" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="92" mass="10282" checksum="6FBCED8D4168FA20" modified="2022-05-25" version="1" fragment="single">GQWNDSVNNCVYSQWEVTVTNHLPRKITNLFIATDDSLTLRDSASIWNIAKNGNVLSLPAYQPSVNPSASYTFGFIIKGTHQPHMIIKAVTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4L6</accession>
    <name>A0A8J4V4L6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glucosamine/galactosamine-6-phosphate isomerase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_001202</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the PIGL family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glucosamine/galactosamine-6-phosphate isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000027">
        <property type="protein sequence ID" value="KAF2077503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4L6"/>
    <dbReference type="OrthoDB" id="7663298at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004342">
        <property type="term" value="F:glucosamine-6-phosphate deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006044">
        <property type="term" value="P:N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01399">
        <property type="entry name" value="GlcN6P_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10320">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052960">
        <property type="entry name" value="GlcN6P_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003737">
        <property type="entry name" value="GlcNAc_PI_deacetylase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004547">
        <property type="entry name" value="Glucosamine6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024078">
        <property type="entry name" value="LmbE-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002557">
        <property type="entry name" value="PRK02122.1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42892">
        <property type="entry name" value="GLUCOSAMINE-6-PHOSPHATE DEAMINASE-LIKE PROTEIN BT_0258-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42892:SF1">
        <property type="entry name" value="GLUCOSAMINE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02585">
        <property type="entry name" value="PIG-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102588">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucosamine/galactosamine-6-phosphate isomerase" evidence="4">
        <location>
            <begin position="36"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="356"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="364"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2077503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="700" mass="79129" checksum="AC1CA3084CD12768" modified="2022-05-25" version="1">MVSGISTINKETGALECHKEFTSKEKLKTLVYQYPDQASAYIANVIVDAIQTANNQNKPLVLGLSAGSTPTTVYDVLIKLYKEKKVSFKNVITFNTDEYYPISRKRIQSYYRFMQENLFEHIDIQQNNIHFLNGEVESQEELDKHLLDYENEIKSVGGIDLLLLPIGKRIGFNEAGTSPKSVTRLVDLEQNTRIDAASDFFGVDHVPHHALTMGLQTMYQSKKVILVAFSEGKASIVQKTIEGDQTISIPSTIFQQHNNTELVIDEAAAVEITRCKAPWTIHSTGAPIVIKWTPILARKAVIWLSLKLSKPILKLEEEEYHDNNLSSLIKTYGPCPNLNLKVFRYLQSTINGWPGGRPIPPQVQPSSPKLSSSTQNLSPPNRLGPLPVQVQNDQWDTDILSQQPISKRVIVFSPHPDDDVISMGGTFIRLCDQGHQVHVAYQTSGNIAVWDDDAIRFANFASEFAKLFNLDDKAKAQSKAIEDGVEDFIRSKEPGQPDSKQIQMIKGLIRQTEARAGARYAGVEPHRIHFLDLPFYETGAVKKKPLGEEDIEIMVKFIESVKPEIIFAAGDLSDPHGTHRVALKAILAAIERLKHNDWMQECQVWLYRGAWQEWEPERIEMAVPMSPHELMRKRMSIFKHQSQKDVPAFPGTDKREFWVRAEDRNRATAKIYDKLGLTEFEGIEAFVGWTIDNKFGTNPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4X3</accession>
    <name>A0A8J4V4X3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ILEI/PANDER domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_004689</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000170">
        <property type="protein sequence ID" value="KAF2074012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4X3"/>
    <dbReference type="OrthoDB" id="20902at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039220">
        <property type="entry name" value="FAM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039477">
        <property type="entry name" value="ILEI/PANDER_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14592">
        <property type="entry name" value="UNCHARACTERIZED FAM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15711">
        <property type="entry name" value="ILEI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="ILEI/PANDER" evidence="6">
        <location>
            <begin position="27"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="ILEI/PANDER" evidence="6">
        <location>
            <begin position="167"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="ILEI/PANDER" evidence="6">
        <location>
            <begin position="363"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="ILEI/PANDER" evidence="6">
        <location>
            <begin position="484"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="ILEI/PANDER" evidence="6">
        <location>
            <begin position="636"/>
            <end position="712"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF15711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2074012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1143" mass="126651" checksum="A7161A861F6CED57" modified="2022-05-25" version="1">MSQSITIDSGKIVYDDTIFNFLATNCNVFIFSPNNEPPFICRNFELQNEEVAKELIAFIDSAPIGYLVMANLINAYYMSDNIKIAFASIGSANIFRYNYIDSAYSKLAIIGYKGLSMGSAQESWTPKVSMNLPDPIPPQMQFVDFGVKNLTPFFNINGIEMKLAYGNGLNVVVLNRKLQIISFKSFNILINSATNYWSYQRMINYLEAIDSESIVCILSQNWSYSSSNANLKTLSTYLKNNFRCTYFDKFLANLNYNWVFMGKLNEAPSVEYTSKDSNTARFYPPLDGSNPVDISVSASSSITNEGGCITRVAGIQSGAVNTYGFTMTLVDDVLGVVIAQYDIIVDNNTGLPNQLTIGGAVGLIQNNLAPGTFVVIASATLPDPFTMNSDLINAFKALGACQAHKISTKTSYALIGRKGASQGSVPEMISSNSTVSLYSTFNVVNPMVKPYVEIKAISKGSTSTSGWAYFSINSIQIPYIANKGFNMLTINETSGEIIDIDSFDITISTEVTRMITKLNALPDKKYVALAVKDVGAQNIPSNLLSVLSSVLMVSNLQNKVTDGASYCCISVARNQFSTVESYETTKQTTVFQRLPILKTVYPEAQQYNCVKIDTSANILISSNSYGRLIENMKIYNFKSNGQTNANSNNATTIYDYIYSLSIGDIVMISCTVKISVTETLKRAMEMIGACKIVHAMRQASNAVRYCVIGRKGCGVGNAMEYYNLDIADTGIAESVSIGYNVPIYPSTTNNLQSDPPVLSFGGLAYYGVPQYNQDLNANVPITVDDFNRERGNIVGAIPALNPKISTPQKRKQRYTVTIFAIITGISYDRTIQEIELPLATKYSRLQYVRLSRYLAVRYPDTQDTKYNINIRTLVEIESWRVQNVALPVSNIRPTITNLKLLMVAANLILKKGDVFYFSFAGHSSLPASMVPQLMFLDEDSEYFVVRLGGFQNLIRVGGGVSVSNSYFTCFSAGQILNPDNSLIQFETILNGKLMAMCTCDRNNVSTLIPYVRYVDYLLNLDLTYGEIFQSMFPIDPAHPNAIPQLYDYSNCTNRPFLCQIALPDPDPNSNELISRNTTSMMINPNEIEFKLASLIASIVELNPSFNIRDVNFEKVFKLLHQIDSNNKSKSSSSSTDRFKYLEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V507</accession>
    <name>A0A8J4V507_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">dUTPase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004574</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000164">
        <property type="protein sequence ID" value="KAF2074132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V507"/>
    <dbReference type="OrthoDB" id="13808at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008829">
        <property type="term" value="F:dCTP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006229">
        <property type="term" value="P:dUTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011962">
        <property type="entry name" value="dCTP_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42680">
        <property type="entry name" value="DCTP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42680:SF2">
        <property type="entry name" value="DCTP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22769">
        <property type="entry name" value="DCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="dUTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="27844" checksum="5066D06421506368" modified="2022-05-25" version="1">MEEKSLLSDKAILRHIEKKTIVISPFFRQNLSTSSYDVTLGPYYYREVEPEPCHSLYNPYSESMVKKIWGEYHQAELVKDWSNRTGMKLENIRDDDYIIFIRPGETILGHTNEFIGGQETVTTMMKARSSLGRNFIEVCKCAGWGDVGYVNRWTMEITNNSKHYSIPLVVGRRIAQIIFFDTEGIEGPSYESSGKYQSSNDIESLKKEWSPQCMLPKMYKDKEITCKVPAFSFDNYNFNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5B0</accession>
    <name>A0A8J4V5B0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glucosamine-6-phosphate isomerase</fullName>
            <ecNumber evidence="6">3.5.99.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Glucosamine-6-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_006953</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the reversible conversion of alpha-D-glucosamine 6-phosphate (GlcN-6P) into beta-D-fructose 6-phosphate (Fru-6P) and ammonium ion, a regulatory reaction step in de novo uridine diphosphate-N-acetyl-alpha-D-glucosamine (UDP-GlcNAc) biosynthesis via hexosamine pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>alpha-D-glucosamine 6-phosphate + H2O = beta-D-fructose 6-phosphate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:12172"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:75989"/>
            <dbReference type="EC" id="3.5.99.6"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glucosamine/galactosamine-6-phosphate isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.99.6" evidence="6"/>
    <dbReference type="EMBL" id="AJWJ01000344">
        <property type="protein sequence ID" value="KAF2071729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5B0"/>
    <dbReference type="OrthoDB" id="7663298at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004342">
        <property type="term" value="F:glucosamine-6-phosphate deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006043">
        <property type="term" value="P:glucosamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006046">
        <property type="term" value="P:N-acetylglucosamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019262">
        <property type="term" value="P:N-acetylneuraminate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01399">
        <property type="entry name" value="GlcN6P_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000002">
        <property type="entry name" value="Glucosamine-6-phosphate deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01241">
        <property type="entry name" value="GlcN6P_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004547">
        <property type="entry name" value="Glucosamine6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018321">
        <property type="entry name" value="Glucosamine6P_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00502">
        <property type="entry name" value="nagB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11280">
        <property type="entry name" value="GLUCOSAMINE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11280:SF5">
        <property type="entry name" value="GLUCOSAMINE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01161">
        <property type="entry name" value="GLC_GALNAC_ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucosamine/galactosamine-6-phosphate isomerase" evidence="7">
        <location>
            <begin position="20"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2071729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="265" mass="30081" checksum="7C5FCDD197FC2FE6" modified="2022-05-25" version="1">MRLIIKDNSVQVGEYVSQLIKKRILDYLKQRKDNRPFVLGLPTGSSPLPVYKRLVEMHKAGEISFQDVVTFNMDEYVGLDRDHSFSYHYFMWTNFFNHIDIKKENVHILNGMAGDHVQECADYEAKIKSFGGIDLFLGGMGVDGHIAFNEPCSSLQSRTRIKTLTKDTIIVNSRFFENISQVPTQALTVGVGTIMDSKEVVLIVTGHSKAMALYKTIEEGVSHIWTASAIQMHQKAMIVCDEDATDELKVKTVKYFKEVESNLTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5M1</accession>
    <name>A0A8J4V5M1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PA14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_003935</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prespore-cell-inducing factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000131">
        <property type="protein sequence ID" value="KAF2074747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5M1"/>
    <dbReference type="OrthoDB" id="14563at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011874">
        <property type="entry name" value="Fibro_Slime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011658">
        <property type="entry name" value="PA14_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051154">
        <property type="entry name" value="Prespore-cell_inducing_factor"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02148">
        <property type="entry name" value="Fibro_Slime"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31137">
        <property type="entry name" value="PROTEIN PSIB-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07691">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00758">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PA14" evidence="4">
        <location>
            <begin position="103"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2074747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="260" mass="29757" checksum="C0379229B78D1597" modified="2022-05-25" version="1">MYQTFYFTNKNTKVKNVQVTFRDLAPETNPDFEMSNSNQVILGLVKPLLNSTDRTPVYCCGDNRAPSATEYVINNQSTFYSWFHPEPNVNVEVQGVLRFTQNITGDDRTMYSYSNDAFFPLDNRGFQNPNYQGPVKSKAYPDSKGVQRNFHFCMDMHASFTYFGGETFFFKGDDDAWLFIDNKLVLDLGGQHDSHGEKGQGLVKLDDIKLKVGKMYPFDFFYCERHSSESNILINTSINLECRFMDFCGVCEGTGKCLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6B2</accession>
    <name>A0A8J4V6B2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">POT1A/B-like OB fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000090">
        <property type="protein sequence ID" value="KAF2075662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6B2"/>
    <dbReference type="OrthoDB" id="2186770at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000783">
        <property type="term" value="C:nuclear telomere cap complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098505">
        <property type="term" value="F:G-rich strand telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010521">
        <property type="term" value="F:telomerase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032210">
        <property type="term" value="P:regulation of telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016233">
        <property type="term" value="P:telomere capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028389">
        <property type="entry name" value="POT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057620">
        <property type="entry name" value="POT1A/B-like_OB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14513">
        <property type="entry name" value="PROTECTION OF TELOMERES 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14513:SF0">
        <property type="entry name" value="PROTECTION OF TELOMERES PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25507">
        <property type="entry name" value="OB_POT1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POT1A/B-like OB fold" evidence="1">
        <location>
            <begin position="347"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="511" mass="58499" checksum="EB9EC95C04344194" modified="2022-05-25" version="1">MSKLTDYKYRKLADLDTSILNDFYCFCINKSDVRKKSSGTFIAMLVADGPSKEKSAIKITLFFESSDFIEAIQFGTILRFQNMKTSHKEGGIMGIGELERGKYNFHCLVINQETGERLSKTPNYSWTKKDAEIVEEMKALWTEGGVKEFWSTPSNNNPRIGATPDPNRPIPIKTTFVKIGDINKSMNFVSILVRLLDKSSNVGPTGKRATLKLWDGTGEGYTHGFDGTTSSSTLGSFIYANTWDQDLINLFDEMNIDAWFAATGVKVNSFREVLELKFFKTTKVIPLSDEDPLVLSALRNYKIRVEGDATNPFTDKSNNNNNNINNNSTITKDDEQVVESWELPDNRLTETAYPHIPTIKIIDILNSDKVPMKFKIAVRLVNHIPSVIQQFTRIRCRSCKHISTHITSNFTFCLNCQSDETEYVFYFKTILRDQTGQIPVIFYEHTNAFFNLIPDDLFRNKEKLAHIENKIKLLKKPDMLFECCIMSYYQADKERTPENVLYQIFDTKLIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6E7</accession>
    <name>A0A8J4V6E7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_005930</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000250">
        <property type="protein sequence ID" value="KAF2072764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6E7"/>
    <dbReference type="OrthoDB" id="263283at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057709">
        <property type="entry name" value="DUF7949"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF29">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25820">
        <property type="entry name" value="DUF7949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035155101" description="EGF-like domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="5">
        <location>
            <begin position="438"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="4">
        <location>
            <begin position="651"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="3">
        <location>
            <begin position="799"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7949" evidence="6">
        <location>
            <begin position="1049"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1019"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1027"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1075"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2072764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1075" mass="119925" checksum="6C84DFC59C955D61" modified="2022-05-25" version="1" fragment="single">MNQKSFALLLVVLYAIVGVCLSFIFDDVTPPLVNDRYAEYLTTTSSVCKNNYEIILVANNVADTLDITGTSETVWKRELVYSDVNVGKYLYSLSIELPSSPTQTTFSIRIQENGVDTVQVVNYLCPEPPTPMMEVISDTLQYDRSGQNFGEFPYFLMNITNLDKAQPPPMTAGFNVVSQTLVNPVVQPLGANIFRVGFSFSTNYNWDDIDIDIYMRMRYPNGPNFYGLNQLSSFLQQESNLIDFKFTTYPQDYILDEGAVAITNIIDTVRTSDQHPFLMSKGNSMVGNSPFCIFQDNTDKSMIKEKFFTVSLPVSEGSNLVTIVHVNNSQLFQDMVDITYTKRSKDISFTGSSHYFNVESYTLSTSFQTSSYIGITVMVGNFDSTNSLPTSLPYGLERGNANDYIFQVDFVASKFAAGIKVDFNDNGPTVPQALDPAIVDTAAPVVHAISHRYVDNFMVIQMNIQDDYSGFYDILNLGSARDIISGNTNSGVYEFLLDLTYRPLAEFIISDTVNNNYKLMKDKYLTTGLDKLPDVHLELAKITNITFKYSEIDVSTGPKTNVLYIHYPSLYGPMRPTIEVSTSVLVSKTKIFYGVYDYNIEAFKFNIEIQKNHINGPFYYSLAIKDQIIFSPQLRDIFGPPAEFNIKSILGDAAGPIITTVQLLPKDVTIPSGVQTNVTWRFTIEDSVNGLKNGTIVIQSSLDLVYYTIEISPESKYFLSGDRNIGIYEVSIPVNGKCKSQKFSIFSITLFDQQEFASFHSIFSESSFYDPLMRFIDQIEAVTVINTMCQYTQTESNPPQLVSFNPKTIDVTYSGLLLQESPRLVTFDFEAFDESGIMLSVPPKIYLFDIRQKSVQMDATLVSHNDTNAVYQCKFEIPYGFGFSSGISVALYGIVDNQSNFKGYPSTQLPVSTINVLPTLNMNISILSTTPLSTKGGSLNIFGKLFSLMDKLIIQDKDMKILQTLGQNASSNSLFIVNDVQSFDMEFVYLTMKRDYSDPENPQYSNTFKLFLQGYVPSSSSGAPSSENGQDSLSSDSNIPTNSPQSCINLCSGHGDCTSNGCLCKSPWVGLDCSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V6W6</accession>
    <name>A0A8J4V6W6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SAGA-associated factor 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002425</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000067">
        <property type="protein sequence ID" value="KAF2076247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V6W6"/>
    <dbReference type="OrthoDB" id="21678at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006994">
        <property type="entry name" value="TCF25/Rqc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22684">
        <property type="entry name" value="NULP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22684:SF0">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT TCF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="50"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="120"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="205"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="306"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="556"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="486"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="69"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="205"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="250"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="572"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="596"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="633"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="665"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="689"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="707"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="724"/>
            <end position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="735" mass="80113" checksum="8003161B12C950E6" modified="2022-05-25" version="1">MIITDQMDSPATLNVGQVSSVISPLQNDSGGGGIPTSDIIMKDIATTTTSAHDIETKENHDSVPVSTLNGDSSSNNSDSDQLKPFLPLNSTWEVIGDLIQNLTGDLKLKNENRIIEKESFLQQQQQQQEQQNIDVDEPVQNNVPVYQDDSDSSSSYTFLHEKYMDIFGLLPSKDPLVLAVCNKCNRPIKQSRFISHYEQCSENVNKKNNNGGSNNEQSNSSVGDNQVSPTDPKKKANGMGRGRPKKKVETTSNTSTNNSNGSTASITSTIPNPPVSTSSSTATTSSTLQQQQVVPTVSAVVQNTPSITNPITATPSPSSTTISTPLIDQSKKQQQQQQQPISARKKNNDLSSSQLRSSSSTVPSISTPAPSKDDMIVPESNTVKGTNSLNNTNNNIISSSSNNNNNNSTCAPINSLRISIVPLQKNKPNKLSRLFKLGVVKSIITITNEDQGDALSDEETESPIQTFIGTTPSFIAEYTLNAFGKRQQLLHQQQQQQLLLQQQQQQQQALLLQQQQQQQQLLQQQQALAQQQQQQAALAQQQAAAAQQNQFKSPAMQNITTPTPTTVPPPVTTVAPPVTTVAPPITTTTTPVHVNNPLTQANSPMAPSTPITPSPQNTPTIQPTTVPLPPPVATTTTTPQPTTTTTTTVATPTPAPQPKAKKSSSKKEKEKKEKEEEKEKKVSKKSSSKKKEEAEKKSSSSKKKSSKSKEKEKEKEKEKEKEKERRKRKKRKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7B6</accession>
    <name>A0A8J4V7B6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004918</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000183">
        <property type="protein sequence ID" value="KAF2073784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7B6"/>
    <dbReference type="OrthoDB" id="21593at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="250" mass="28371" checksum="340B41B8754C72AE" modified="2022-05-25" version="1">MNDSATLHQSSPFFNKLKRRIDSENDHDGFHSKRFLSQSMANKIGILSLNDDDYSFQNNSNNNNNNSSVNNNSSNIFQNNSIFQQPTSMNINSNDIFKTSIIPEKQNLNRNDKNNNTIFNNNINNNNNSPVPATINNSMAIIPVEKIKSTESSPKLIPKIETLWDLVTNISKSTYPIIPPLTPRSVGEDKSRALILYSEPSEVIQGSLRKEVIEKRKQQLKQNQDLKPLDMEIDTNVHGININKTNKMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V832</accession>
    <name>A0A8J4V832_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein-serine/threonine phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_001266</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000029">
        <property type="protein sequence ID" value="KAF2077417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V832"/>
    <dbReference type="OrthoDB" id="285418at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000004">
        <property type="entry name" value="Protein phosphatase Slingshot homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF414">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="8">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="154"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="216"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2077417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="303" mass="35239" checksum="CCF570E8C76078B8" modified="2022-05-25" version="1">MFFRESSIIDSTANDEIDSMALFNLLQEFPNNKVIIDCRDTESFSKGHIRLAINTPPPGSSPIDYSLFDIDKYIKENYEASFWNTAHRKVIVYSDKALSLNTDIIDTWEKCVLQYFISNKKKTNVLYTFTGDFESFQREYPFMVAKFKITPELYPSEVIKDFLYLGGETAQCLNKLKSLKITHIVNMASEVQDSYTHLYKYYRADVDDRVSANIDEHFQPIIKFIEEAKQSNGRVLVHCQVGISRSASVVIAYLMKTMDTPYFDTYKFVKSKRCIIQPNPGFVRRLKAFDEELTKERKKKLNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8I6</accession>
    <name>A0A8J4V8I6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003609</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000115">
        <property type="protein sequence ID" value="KAF2075089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8I6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2075089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="116" mass="13502" checksum="5838086582DF6E91" modified="2022-05-25" version="1">MKLNVYQDDVFFFKNENIPVDVRGLIFYIGTVYHPKEEEQQEQLYNYDTLKSDLISIVSNLPSQISHLDFIIRSDHIGFFPLYKEKYVAVPTSVTHLSVSGVNLSRDIPFLHQSHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V966</accession>
    <name>A0A8J4V966_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000217</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000004">
        <property type="protein sequence ID" value="KAF2078467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V966"/>
    <dbReference type="OrthoDB" id="10290201at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF180">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="540" mass="62146" checksum="9896D75924589E5C" modified="2022-05-25" version="1">MKDNNLFISIYRNCYLRSLIRNNVFKDTIICIPSLEYLNDNHQYLSVFSNDDKLQYNIFIQYTFNNKNSYQQFSHNSNRHLVNDLEWGKHDIIDFGILYDQVHRLSFCIQQDSTISNVDKLPESTIVELNIDTQTHTRFVCSSLEKILLDLPKNLKVLKLSRLFILSSSDSKIQLPNSLVKLQYSGTGTELDRFVIDTPNKILNGPCLKVYSTQDLQWLQDKLWISDILMIQGVHQIPSHVRKIDLCVALDKELVHCLPPRLEWLNVNKIRGPKNQQHVALLLKQHLSKLRHLSLYVYIENQLGKEPLFSESLEYLYLSGGHKEIDGSMFPPKLKILNLGQYNRELKIGSLSNSITNLTLNYYDEPLTSSVLPDQLQSLCLFSFDNTIQANCLPTSLTSLQLNIFSGSFENVGTLDNLRYLKVHTLNQSMVDTLTNVNRIKLSFFNIAPNTSFITNTSITNLYLQNTSYDGSTIADGFLPSSLVRLHLDGFRIQSIDTIPPSCVYLDYNHKNLDLTLIPQSVKNIYFLEPKVLDTFIEYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJD9</accession>
    <name>A0A8J4PJD9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_010130</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-12 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000937">
        <property type="protein sequence ID" value="KAF2068545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJD9"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044986">
        <property type="entry name" value="KIF15/KIN-12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37739">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37739:SF8">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Kinesin motor" evidence="12">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="180"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="347"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="576"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="652"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="183"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="777"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2068545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="777" mass="91153" checksum="EF4ED2C51B9E5131" modified="2022-05-25" version="1" fragment="single">IDKNKGVYVENLKQEPIDSNQLAMDLLKQGISNRHTESTSFNETSSRSHSILTLNLESKNKSNKKILYSKLNIVDLAGSERQKNNNNENNNSRIKDASFINKSLSTLGNVIRNLVDISNGKPRHVPYRDSKLTLLLKEALGGNSKAYIIATISTLDHCFSETLSTCQFSQRAKSIQNTPTINHKQDEKQDEKKQQQQQQQQQQEQESIYNLKVQLVNSLEREEILKKENNKLLEKIDFIQDSNEKKDQYISNMKSILKYRDQDIYRLSCSSPSLNIINNNNNINSLKETKSFQELKLENEILYQQILYQPDLNILLQENRELLDIIKEYQEFFNDNQDQDQDPFNYFQSLKQQNKELVSTINNLMQDNNNNNNNLILNSEMIKVELKIKIIENEKKEMESQQQLLKKELISSESTVQVLEKQLKHLENKCDLLIKEQSTRKTIQDSIVTDTLDSAFKQFKKNNKNPKNYNQEYIESLEFRILLAEQENQLLSKSNDSYLNQLFENTKKLNDLYLLLEAFNLSNQKMSSFFISSPLKLQESTNVIDKDEIIKQLKYQFNEKNENLSNRLTNYNFQDVLQLTSKIHQLENQLKEKDKELQIAKENYQYSIKKSQLEYQDEMEKKLNLLLQKVKLENLSVIENLKKDLQSKNTIYNQYLSINDTLKKDKKEQENEIKYLRNQFETLNNALLVKSREIGKLNDQVGLYRKRYPKIDSDIIIISSDDDNGDREIIDLSSDSDLEIDLSKLTIKQEKDDNQKFNLTGQELIDYIKIKQEKLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJI0</accession>
    <name>A0A8J4PJI0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-phosphatidylinositol 4-kinase</fullName>
            <ecNumber evidence="2">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_010127</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19877"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.67"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000935">
        <property type="protein sequence ID" value="KAF2068547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJI0"/>
    <dbReference type="OrthoDB" id="10264149at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05168">
        <property type="entry name" value="PI4Kc_III_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000016">
        <property type="entry name" value="PIK1p Phosphatidylinositol 4-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057754">
        <property type="entry name" value="PI4-kinase_beta/PIK1_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049160">
        <property type="entry name" value="PI4KB-PIK1_PIK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF22">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21245">
        <property type="entry name" value="PI4KB-PIK1_PIK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIK helical" evidence="7">
        <location>
            <begin position="57"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="6">
        <location>
            <begin position="793"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="284"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="472"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="690"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="286"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="315"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="328"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="340"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="350"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="370"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2068547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1078" mass="121783" checksum="218D67C513EA89F8" modified="2022-05-25" version="1">MSEDVSTTVLNSEITSNDNDEQSLASTTQSLSSTNISTNDDVDNDGGIKATTTEDSTADLVIVEPIQNTATTNVIIPNTSSPMSIPIITTTTTTSSLSAPTSKPTTPTKAIVDKEIQEKREKKKLKKKILKQKEMENRDRIVKLFSGKFTSWMAVSHLYKYDYSDGITDFLCNKLYNLEDKDIDFYITQICILLVNQPHDQKAYFSSLARFLLDRCAKSIRFAIKSYWIFQAFVEDGEKNLLTIQSSVCLHSPSTSPQDLAVYSNEQMAPIDLDKIYNRSDYEFNNIDDDDDDDDDDDDDQDKKGSKRKSNNNTDKQENIKQDEKESNNNSPSINNNNSKQEDKEEKGDNTYQSNNTNETSPAKAKTTDENNNNGNNNNNKEEGRNGAVVVATMDDISHNYLHPNDPVENIRNILKRNRNIYNNIEEKKELATRLRDYCEMSVITCSRPLITRPRTSSLPSPILSYPISDYNSSSSSSTPFTPISSNNDNIDIENTTTTTTTTNNTLESTSIPTLEQQNIFEKSKTPPIESSPHTMVYDYNYEFELSKSYRCEYLNDILSFVQKLNYISKILVNISIDKRQAKLKNEISLLNIKLPLGLYLPLWQSSFHHCIVRIPPEEVKILNSRERVPFLLVLEVIESDQEAKTNNIFEVVSSYLQYTTSASNNLQVMDQMKKKYFSYTIKKGLLSSSNSNLQPSTSDSSDSISNNLSDLSSATTTATTTTTTSNNTTPITSPPTATITATTTSSSSSSLSSTPPINNQINQLSTSSPTTTSPTVEKIIMDNSYNSSPFGESWEEKTQRYKRISPFGQFPNWRLCSVIVKTGDDCRQEQMAVQLIEKFAEIWKESKLPLFLRPYSILVTSSSSGIIETIPDTISLHNLKKNVPNFTTLFNYFKSTYGDVNGLPFRTAQTNFIESMAAYCIVTYLLQVKDRHNGNILLDREGHIVHIDFGFILSNSPGNISFESAPFKLTQEWVDVMGGVNSSQFQYFKVLCIRGFVEARKQMDRIISLIEIMMSGPKMSCFVGGLECIEQLKARFFLNATERECDVLVENLITYSLDHFKTRYYDKYQSWYNGILP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJN7</accession>
    <name>A0A8J4PJN7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010597</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001228">
        <property type="protein sequence ID" value="KAF2068077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJN7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32556:SF18">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32556">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="435" mass="51313" checksum="F5941A5ABE617013" modified="2022-05-25" version="1">MTLPIPKYVYTKILDYIYYSDHRYNQVKKVSIQYALVCKQWFQISKNLVNSNVIVDLHDLEKKHSETNTYRIITRESVENIKFGFFHINNKLVKSLNSRFRSLKSITINHDMRDYWMGNSLGLLNRLNKLKRKDKIQVNFDLEVHCFDPGDPDQVEEEDNEDEDEESEGPFEFNTVDFKFDFDIEYYRSYEFCYELIKLTNPKTLEFLANSRTDSGASHLTLYHSISKLNNRFSSVIINDDYIPLYALYRFLQSPHLQTFKFNLQFHFIAALYDSDSSDNNSYDFNQMDNFDSNRYLPNKVNEDGQIYCSFSRGDETDSTIPIYSKQLWNQCLQLLSNNSTITELSIGNIECGYECLYHHDDNANIFGQQLYDDLLDALSNNKSIKSLYFDPIFSPDINSAFLTSLLERNKILEKIKIKQKFQPITRIRKNLRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLK5</accession>
    <name>A0A8J4PLK5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008471</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000525">
        <property type="protein sequence ID" value="KAF2070210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLK5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31550">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="575" mass="67193" checksum="013F48071A42B140" modified="2022-05-25" version="1">MIDQLFQKVFNNKFISTTIYQNVHQIQINKNSLKYQDIVDIGWMFRNGHKGLLKEKLDNNSYLYLDNKDLFSEIANKDTELFIQLFKQFKQGALEYYNQAFNRNRLYFLVDLVNIDVVKYLHENGYAQKISSHVNIRFFEFDINILKYYLENGLFSPTLQNLIPFVTIQKRPTPFSKEMVDLMVQFIPSPISVVVAKSFLDSLVTNIVPYVFGATSKLFDQGIQSCKFFGNTFTYNQIVEIIQKKEKELCLKSKLQKEKGFLGANCHVDAVELVANFWEIRMLYGQQAFIDKWKSLSNRIRVTSSKIYFATTTTFCPDDITDDDISVPEIGVDILSACCVTPSLKVIDFISSLGYSDNLIMDTMAGHMHPFLIQLVAITDQNERDIIVKLCKISSSFMIDLIYYCCQQTNVESFTYYFTLFKEQINEDAIHEYYECALNHRQYEICKLMESHGYLFSKFFDTNNRFHLIYLYLSNTIQGDSKDQVIQNKLLNIAIDSNDFFGLKYVFNNYQCESFDRDTLIKIGSRVYNLSIIDYICKNACFSDPNFINELIEIATINNNKILIQYFINEKYLYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM21</accession>
    <name>A0A8J4PM21_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PiggyBac transposable element-derived protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008193</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000484">
        <property type="protein sequence ID" value="KAF2070495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM21"/>
    <dbReference type="OrthoDB" id="21404at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029526">
        <property type="entry name" value="PGBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13843">
        <property type="entry name" value="DDE_Tnp_1_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PiggyBac transposable element-derived protein" evidence="3">
        <location>
            <begin position="307"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="173"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="501"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="89"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="31"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="173"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="187"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="200"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="600" mass="67990" checksum="8DAA592B6DC68447" modified="2022-05-25" version="1">MNTLSSRSSFQFPFDPVNSESNNTKKNMVPFNYNNNNSFENSSSPISSPSSKTTPSLLTFSEKDNDFMISRRLIPPPSLIIELLLLQQQQEVTQKIHHQQQKIQKQQQEITQQQKLFINNQSNNNDIKFSPYKKSISRNIFEDKNNNNNCIKKDIGSTNLLIEELKTIVNNNINNNNNNNNNNNNNSHSSPSSPNQYYDPLTLSSSPSPSPTLLNQPLYNISPSSSLNNLSSRCSSDSSSPISSPRSSPSSFQFNVNSVNNIDQDQIFKELIDQSIAQSNINNNNNNYINTNNDGQVFSKLKSSHITPLECFKFIFKNTVEFLTMEISNIRKSNSSSTFYLGSRFPITSKTIESFIIVSLFINSHSLDRISDYWSTTQAKAFPGVSLIPKILSRNKYFEIKSSLSHVLSPNLLDNIQNLLYKDITYQFELFFGSRQDIESIQSTKFKTPVKHRNSTKELAAFNLILEYGLSNAIDLFTKYRQCHTTVPQFVEVLTEQICNSEESSSPNPSLPQQQPQPQQPNSVLPSFSPSSPSSSSNYNPTSFHSLVNSTKKKQCFSCKFPIQSSLNSQNNQNTTLSTLKCKQCFLHYHKECFIASHDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM98</accession>
    <name>A0A8J4PM98_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_008076</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000469">
        <property type="protein sequence ID" value="KAF2070600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM98"/>
    <dbReference type="OrthoDB" id="10259130at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000039">
        <property type="entry name" value="Cysteine proteinase 2, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0458">Lysosome</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035327880" evidence="9">
        <location>
            <begin position="18"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="11">
        <location>
            <begin position="37"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="10">
        <location>
            <begin position="119"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2070600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="336" mass="37354" checksum="2B28D170BCC05EBB" modified="2022-05-25" version="1">MFKYLLISTLMILVASAINTGVYHHRKFSETEYKSQFISWMNQRKLVYSSENFNQRYENFKNNVDYIANWNEAGSATILGLTDFADLSNEEYRQTYLGITYEAEESDEQLFDQADAAAYPNHLDWRVRGAVSHVKDQGRCGSCWSFSTSGAIEGANFISTGKMTTLSEQQLIDCSKKYGNHGCNGGLMEPTFQYVIDAGGIMTEESYPYTAKDTSSCLFSKKSAAATMSAIGNVTRMSENSLLQAVQDAPIAVAIDAGQRSFQLYKSGVYVEPACSSRRLNHAVLLVGYGQDQSSNSDYWIVKNSWATTWGNEGYIFMSKGSNMCGISTMASYAII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMD3</accession>
    <name>A0A8J4PMD3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008964</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000607">
        <property type="protein sequence ID" value="KAF2069723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMD3"/>
    <dbReference type="OrthoDB" id="24030at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35885:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35885">
        <property type="entry name" value="CARBOHYDRATE BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035272803" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="316" mass="35482" checksum="FB6548C065A917E7" modified="2022-05-25" version="1">MSKLFVGLFITLCICASSSSASDTYGVGIWNNGTNVNVGLVNYSRAAPTILKVVIQQFNFIGDASQISTYNYNTKQLTLVANIINTSQFFLITIDCNTWTIVSKSPISQNVNYYGLESDYSTNGTLFAPTTYGDGLIYMNILDPLSLAVRGTFDYFPGSVKGSVYDPVNQLYYICYTLATNANIRCRQYNSSYSLVQEKQLNVTNSNFQVIASPISLIYNPTTKTITCYLQMMNQYPTPSISYYFTLFDFENSVFNVIQQNHIYGTIVSTASDMNGLRLSYSVEYFNGQYQLWVVDTRFSTLLTRFALNTALLSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN32</accession>
    <name>A0A8J4PN32_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-histidine N-methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.85</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_007736</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the methyltransferase superfamily. METTL18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.85" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000429">
        <property type="protein sequence ID" value="KAF2070945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN32"/>
    <dbReference type="OrthoDB" id="1723750at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018064">
        <property type="term" value="F:protein-L-histidine N-tele-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF39">
        <property type="entry name" value="HISTIDINE PROTEIN METHYLTRANSFERASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2070945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="293" mass="33607" checksum="F76042C2304C27F6" modified="2022-05-25" version="1">MSFKFNFNQDNDDSENKQDDNEEKLREFNVKDDSTVELCAQSCHFTASEVKESDIEIEKVLFKNNYQLKKLMKPLDIKPPHLNKNSNNDYEKLLTKSDLIPGVYEGGFKLWECAIDVINYLIENKINLKGKKVLEIGCGHGLPGIYCLLNGAHVTFQDYNEEVLYKLTQPNVNINCNGDYEMIENNTHFISGDWKFVSDILSKEKFDYILTSDTLYNVSSFSKLHSLISNHLEPIQGKCYLASKTYYFGVGGGIRKFEELIEILDILNVITVKDIKDGVSNVREIVEITLKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNJ4</accession>
    <name>A0A8J4PNJ4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007502</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000404">
        <property type="protein sequence ID" value="KAF2071185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNJ4"/>
    <dbReference type="OrthoDB" id="22524at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="929"/>
            <end position="956"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1213" mass="138175" checksum="E417A591CA13CC7B" modified="2022-05-25" version="1">MGSKEENYDDLFQRFGDLDNYSNYLACVEQSNSFKAFTKITKRREFPENNDNDTQIKSRISQWSMEDEFRKDIRNVTLVDIAAKSLLLLLKTTKTFNQIQLVVLCFHGTKFIKDLTEIVDLLKDVVVSIKVIRNQKAEKGASNELVKRFKQHLKESTLVYQSDYLISFDEFKEFLVMEDTCTSLDEFFNYSFGGPGDDDILPNTFDLIWTSVISPTEYEIPDGVKKLTFTNLKSISGSKRVIPNSVIYLKLSIYNGGLSKIPFSKHIKYLDLGCRLNHFVFGHLDIPLSVSHLEITLVQNIAHHWLTFKGTLSRGITHLKLNYPYGKIEQLLNPFLIPTSVHCLDIGTDNIINFQMVDQEEYYRDPLSRDSFKSIANLNSIELSKQWDIPITINTFPIGLQSLSFLNVYRVCFDLGANVLPTTLTSLKCSSIEQIPDHIQLNYLEYPIEYTFLIFKSNDRMITKEKIPIGFDKSTGVFTPQPALPIDELFLQHFNGKIIIPMPNADIRKVKCNQRMRLPDSVQVYVSNYFLPVNYPPLLEQLYLEFDDPGYKKIELLHSIPPTVKYTSFRFYSTSNNNIFKAIEKYKLSSLDEFMKYNSVPINKDSFFQIWRNVFLQKKINEMAQPKLIYTKETNANGISVYRLQDSKLSSYFKFKTDYISDIPYGVHFYSIKERIASAKQYLLLSLPSSVTKLKVDCYGIKKEMIPSNIKHLVLKSLPFGNIEINSLLIPTTVETLEIKACIGPPFTNVQLLPTTLKQVIIKTKTLQYQDHGKGFINQNVMDYLHSKNLLNNQVYQIYDQSSTGIGPLTTILVWQVNKLIPVGFIPNGIKRIVFGNNFNTTILPNTIPNSVTEINFGTGFNQCLSLVYLPASLKYLSLNGYKHPIVQNSLPLNINQLCLSDYDSRLVSSCFFPKSIKYIKINNKRYRQNNLQQIKSNYENENRELNIHIHDHLNNNNNNNYNSNIKSTKTKVFIDNHPIVPGSLDHVNITSISFGSLYNQILLKGALPDTVTELDFGQSIYNQPILCIPTSVQILNLGSITTDLESIVLPSSLTVLYLPNTFDSPIPTGFLPHGLKVLHMNKCFKQIVPVGVIPNTVKEISLSPHQGMASLDFIPSSVGKLQLHGYKGHHSLLSTIPSSVTELIINTLVSIQLIPTSITRLRCLGNDFLGNMDLLPSNIKHLELDGANFTGQIPSTVERIIITNYDTNLYTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNL0</accession>
    <name>A0A8J4PNL0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MACPF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007467</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000400">
        <property type="protein sequence ID" value="KAF2071215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="21110at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF5">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MACPF" evidence="2">
        <location>
            <begin position="1475"/>
            <end position="1814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1707"/>
            <end position="1732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="2266" mass="254694" checksum="50AD1974CF4BDB82" modified="2022-05-25" version="1">MGYSFAIEYKNSTLRTYSDRNLSPVGLISTGCTSLQYYVLFEGTETIKNITCNQEGISSFSYALIKTVSPKRTYEFTFDVTYQLDTSLLSVIVETQTKNETFILSNSPISCKGLSQLQFQSQPILIVKGSKLEISLATDIKQFFKSSFSCTIFDDTWSTTECTVAQSDYSPDIFILTTPITQEIYSKDVVNINLSFDQNAIIQLKDIATPFIAGTTTSLSFDSYPVNKAIENDYKTGKRSSIVLSTSSTNDTGIILANGQVISSFTATFGNVDSKTFHSFVNYDLASVVVDPIIKVLYKNADGVQESEITIDPTLATSSMNVTANVSNDSSQKLPILKVLAKDVIISNPIQVSSKLGNLEIPFPFGIKKDGAKYNYVSDQIFPFVLNSQNTVSVNNKRFVQDAQVVIPATSPLTQDVTAPSYSIKSKKSYDNFITYQLTITDQTSGVYKIRCINTDQEFFAADTIISGSVTNGVFEFTIDLARLVSSSSKLILHVTDLYSNTNVYQGDSIYLDDNLNYPFDLFSTKVYLKDIIDFEFNSNLFDTSFKAKKNSLFFNLINGDTSFKPIIQLSGSTFIGSFDQVVQKYRIDFDLPFKPSVQVLDYQLVFPGSAFSFNSLQSHFGDVAIIKFINTDIDSYPPMFSKIDYTTGAQQVILSSSSENKQVGWRFTIVDKPNGFKFGNVTVKSNLNPKPKTYEFTTDKRVSGNSISGVYELKFEVSGASPSQEYMISSVILVDHNGVASYYPPGENQLSPFMELEGYYHSISVVTPRVVETVLPTLRTFNVLQIYRDSYRITFSVTDTGSRISDENPPIIYLIGYGGEKQEIVPTLDRITSSNTVYNYEVTFNIDYLLAYHGCLISLYGIYDNDLNINGYSSLDLKSMGYTYYIKKTWSVDPIVSSFTSIGQSGGLLTIYGKRFKSSSSRIDYRTTVTPIYVSTNVIVYDYPATLSSQPITVTSIEGVDYTFNIKPTLPLVPTNNVLYVDTLSNCKTNCGSYNLPYPSLSLALKNSKERTTLILKDGVYKGEDNMNIFIESAYTNIRPMNSTSKVVFDCENYFQLFNVKGSKSFTLSNVTITSCTSSKGGALLLESSVVTLQNIKFLNNKATNGAAIYLLNTELKLVNAVFKENKAITSGAAIYSYLSSVNIKGYFTTFVDNTNFNTGTQSRDILCQNSTLNIDDDVYIKDINVKCLQGCDNFYSQRDICHDKSHEISDDAVCGDNVCSASESCLSCKNDCQCYIEGMVQETFEPGCSPTYFIPDNLFENGTNVKPCVPTKRTTLPVVHIENFMGGIKNVVVRLFGYVSVESSKEVPFNFYGTNFGLIFKVNGQQQFYFNQNTFFNETKTLYLVDKHVHFIEIILFTSVVESSERTFTLAPFEDATTKLFYSNLVCGDGILNEREAIEPTEESNPDESYYCITDLGYPIFQDQPKCGDNICNEEPNSCFQDCYIEYTPVCSATKVIEGAISPGFSVGEDTLGLLISNQFIWRLPGSEHLSFGVDITRAEEAMAPIFQFDYCQDAATNVLEDVYRGKLYQIPLEFNGKAVPECTFSTSTESFKTTKEMATSDSKRVLHEAETSFQVTGPAVKGGASASFSKDKTNTSAKKILSSNSQRIFRTDLLCKSTYVELDLNRVSFHPNLLESFNQVQTPKDMLKLVQRHGTHFYKKTFLGGRLSQIAVTDESNVDESNESSFSESYKAAFSASVSAPAFSVKTSNSGSLDRNNDEKTQQTRSDQSTFSKIITYGGVPAAFSPAQDGASSPAFGEWAQSIDRLPVPIDYQLYPISHLINEEWTNQYGMNIKELWLAGEELFYATKSTSESELFGTDYSILFEMLTDGYTDIEPIYKFPVLDIKYYPDPEDLEKESQLLIPVTYRYVDRFGNKYNGLYSNMLSNWSITSDQMSFYQKFEHLNYHFHIINPYFSNEYPPHQNMMAPYRFDFKAPDFINSYKKPVIKLNDNGTPIDFNKEIKIISWSNSLAVILDRNGLKTDSTRAYGTAAFESKYSPFINTNIKFTHQWSQGKPEDFDLITTNVSGITAVRNHHAQYPDDRTFFVECNEDGPIIEWQPIIGIPPSSGHAAKCIEEVTFSFDQQTDSFSFTKNFNEKIAPTTRWHSFNLPVPEDKSIKKMKIKHPDTNIGLASRINWITLLYPQKDFTWKYDVLGYHIKENQIEDTTTGHTGEWITSNYGNNYRSVDLLPINTFARQIFFYPNYNFRYPFYPVPDGWEYPFFHSILNSTETTEISSKFKDTSGEYTFWVAGQPGYVPTPDVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNX5</accession>
    <name>A0A8J4PNX5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000378">
        <property type="protein sequence ID" value="KAF2071412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNX5"/>
    <dbReference type="OrthoDB" id="774557at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029005">
        <property type="entry name" value="LIM-bd/SEUSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10378">
        <property type="entry name" value="LIM DOMAIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01803">
        <property type="entry name" value="LIM_bind"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="399"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="552"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="659"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="838"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="913"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="330"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="368"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="401"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="552"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="659"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="673"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="697"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="719"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="774"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="860"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="889"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="917"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="947"/>
            <end position="960"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1096" mass="122514" checksum="97F249F8CAF42987" modified="2022-05-25" version="1">MFQKITQSPLSQQQQQPNQQLQPNQQPPLQPNQQPNQQQQPLTPSGAPPATPFSGQNGVIKIPQANFMVKNSDSGNGGGSNNNNNNNGSPFSGMQFEMMDVLNNIHKKLKDFTKYHLKKPTDNSITFWNQFIKDYFTEDSIYRYTVPKSKDSNIKKTYELNIITLPRLFQNAFGSSELLDFSFSFDESHMSYDTSLAQFSFFSDRIKIITIYDCVQVHISGVLEVYFNTSYKISLFHFEVTGFQELMTTSKIESLFNSLLTKSFDNNTVSSSSLHEDIFKSKSMNYFITTLNQLSESIENEKIQKENEKSKESTTITSTDVVNTSESNINNNNNNNNNNNNNNNNNNNNNNNNNNNNNSIPSVKLEESTINNSNSSSLNHNNSFNSELTNFENVKEELFGSSSNNNSNSHNNNNNNNNLNHNNNNNSMHHHVGEADIIHDWINMDSHPHSNENDLKLNENNLNLKSDIDSDNILDRNTIYQDMLVDTHINDINNSGNKDDINNNSVNDTNGDTNMNDSIFLESSIDTNTNTPIVDYSMENSTIKHKSDHSLLSSIPINNNQNNNSNDKNLNEINHNNNNNNQNNNNEKKFTIEQMRSLLPCSPVNQITGVPHSLQRCYDIIDTLNTLQPLMKFSIQNKVSVKEALPGFLKFYSNLNNTNNNPNLQQSSNSQTVQGSPNQRLSQPNTPPTNPFLVPNQKISFNTTTPLSPATVTPPISLNTTTNSFVQTSNNNNSTTQPINPTSNGTSTTTTTTTTTSTKPLPPTSKGSKKKRAQSIDKTSDIVIEKPSLTRHSSCDSLPLSSDLLRKATQNSLNNNNNNNNNFEDQFIQSSPIIASTAEVSSPAITSTTKSSSRSRKKSTTTTTTSTALSSSINGSSNSVPNTPPTAPSTPTSSFTPSSPFLSNNAVLGGATMGVINNNNPNNNNHVNNNNSSNSNINGFFPPYYNQQQQQPQPQPQQQQFYVDHQNEFVSPQMNGLHRNIPTHHFNHPSDGKMNNNHNNTQQLHESDRFLNADQVNQYMSEKEGLMNNNNLNNSNGMVMGTNNNINNGTGHFLVNNNNNNNNNNHLVMGNHNSPPVQYDHNNLRQHPYIYKPNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNX8</accession>
    <name>A0A8J4PNX8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cyclin-dependent kinase</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_008695</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000562">
        <property type="protein sequence ID" value="KAF2069981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNX8"/>
    <dbReference type="OrthoDB" id="21240at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001022">
        <property type="entry name" value="Cyclin-dependent kinase C-1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF246">
        <property type="entry name" value="ECDYSONE-INDUCED PROTEIN 63E, ISOFORM N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="73"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="360"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="712"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="785"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="819"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="596"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="440"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="730"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="819"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2069981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="976" mass="110098" checksum="E6D85198A7F6006B" modified="2022-05-25" version="1">MQEKDKQADNIFSTANNISTTTTTTILGDDTEKEINITLPSHQKGLDSVEENYFKNVQQSISEPISQTQTSTFQIIELIGSGSYGKVYKAIHIPTKCMVALKVISVMNIENGLPVEVKYLMKMKNCNKVVKLVEYFFTNDDKLVLVFEYIEYDLWKFISPKNRLLTLSCTKFFIQQLLQGLNHLHNHNIMHLDIKPSNLLIDSSFNLKLADFGFTTWIGNPQLNNQVISLFYRPPELLLGSKDYGPEVDIWSVGCIIVEMLTGFYMFAGTNEETQLELIFKTLGTPTEKNWPGISKLSKYQSYSKSIKKYPTKSLKDLSSKFNNLTQSTLDLIVNMLTLNPKGRISTYDAMNHPWFSEDPTTKIKKYNNNNNNNNNNNNNNNNDNNNNQPSQKETNNQPNQKDNHHNNQHNQKETTNQTLEPNSILIPKRRHSMSKKETNNNSNSVNNNNNSNSVNNNTSDNNQNNSLANNNSLDFLTTKHPLYQKIKNIEYLVLKSEQLRLKVCNTLIPLSNHIQELESIKFLLKEQADLLSFDQTAYLEQKKQDILNQLQPPLIQEFVQPINYSNNNNTTTTTTMSSGTLLSPNSLSRLTSQQKQHSQIQRQQLQQQQQLMQQQKIQQQLQESITSIKSHFLDYQQQKQACINAGDLSLFFTNPVTGAPLIPLPPPPFFNYVPTWTSNQIFPTTFFSSQCQSKSNSSSISSIMSTASATKLETGDNKSLTDEFGNITNNNNNNNNNGNNNNTSTGVSVNNYSPSSASYSSLSTSSLSSSTSSFIYSSYSPSFSSSSLSSQRSNSLSSYSSSSSPVSSFINSMSSYRFSHSGSSAYSSSTSSKTTFKSSTKQQQQQQQQLQLQQQQQQPIVTTATSTSNTTEDNKSNNNNNNTNTNNNKDNNTNINNGNNNNNNNNHNNNNNSSSSGKKKRHLDPDTHILEAIEDIQKQSENEYQQQISTIDRLQKKRKMILEGGSPDYPIHIPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPJ4</accession>
    <name>A0A8J4PPJ4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vps41 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_007194</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000370">
        <property type="protein sequence ID" value="KAF2071478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPJ4"/>
    <dbReference type="OrthoDB" id="20550at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057780">
        <property type="entry name" value="Beta-prop_Vps41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040324">
        <property type="entry name" value="WDR44/Dgr2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14221">
        <property type="entry name" value="WD REPEAT DOMAIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14221:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23411">
        <property type="entry name" value="Beta-prop_Vps41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Vps41 beta-propeller" evidence="5">
        <location>
            <begin position="344"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="351"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="444"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="34"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="102"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="282"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="390"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="128"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="149"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="200"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="289"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="393"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="412"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2071478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="724" mass="79976" checksum="8902C5A97DF03F78" modified="2022-05-25" version="1">MATTITLNSSGSNLTSSGGAQKHMHYGFYKNNETTSSNSLYSRGDDDDDDDAFYDAYETNPSWTPPVSPSYLINNERSSSIPKQFHLDKLCPIDDQPKLEGASIQSSIGSSDASSPEYSPSNTSPPLSSQQQQQQQSSSELKSNTLQVNNNSNNNNNSNSKNTLKAKRNSRVISLIKNKKEELHNLFHRDGKEKEQQVPSISNTSSNNNNLANPSTTNQPISVVALNQSNEENTINVPNSSNISDTTSSCSNLNNINSNTTNNESAGSLRKLIKKKLTSMNLLDGENNSSPPSSLSSSTEISNVNNNNLNSNSNNNSFTEKQDSRVTRWLKNHNLKKKKELTSKFVLCSSIQAHKGSIWALEVCKDENHIATGGSDGVIRIWKIIKSTTPGLSSSNPIMSNTSSSALSDTVSNGSSNSNMSGGSNNLGHVEQPKILENTPRVLLEGHNGHILELKWINQQLLLSSSIDKTVKLWNIESGECIKTFEHNDIVVSISFDFVNNSLSHFYSATLDGIVRKWSIDNSESPVDSIELGEFITTISLSSNPYYLIVSTHLGNLVFFNPTNLELLHKFNVSQNHHNIKRGKGPKILGMSVVSSSLNSNCEYLLVSSNDSFVRQFSLKDFKRICKYTGMMLENYQVKPIVSPDKKSVIMGSEDNNIYIYDNYSEQQPTDEIRGSAGNKIIVENYEYFTASHKPITNNVFVTINSMNYLISTDSLGQIMIFNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPW4</accession>
    <name>A0A8J4PPW4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Carbonic anhydrase</fullName>
            <ecNumber evidence="2 10">4.2.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 10">Carbonate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_006673</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 14">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the beta-class carbonic anhydrase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="2 10"/>
    <dbReference type="EMBL" id="AJWJ01000318">
        <property type="protein sequence ID" value="KAF2072010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPW4"/>
    <dbReference type="OrthoDB" id="10248475at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015976">
        <property type="term" value="P:carbon utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00883">
        <property type="entry name" value="beta_CA_cladeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1050.10:FF:000001">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1050.10">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001765">
        <property type="entry name" value="Carbonic_anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015892">
        <property type="entry name" value="Carbonic_anhydrase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036874">
        <property type="entry name" value="Carbonic_anhydrase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007756">
        <property type="entry name" value="PRK10437.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11002">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11002:SF76">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00484">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00947">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53056">
        <property type="entry name" value="beta-carbonic anhydrase, cab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00704">
        <property type="entry name" value="PROK_CO2_ANHYDRASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00705">
        <property type="entry name" value="PROK_CO2_ANHYDRASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 10" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601765-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2072010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="274" mass="30942" checksum="347BCC1205E910B4" modified="2022-05-25" version="1">MSQANNNNTTNNSNDKSVPTNTPRARSDSTNPLSPRDCGCSSIFNASHGQKIEIETLIQNNHEWSSRIKQEDPSFFEKLAVAQKPRFLWIGCSDSRVPAERLTGLEPGQVFVHRNVANLVIHTDLNCLSVVQYAVDILQVEHIIVCGHYGCGGVAAAYENPELGLINNWLLHIRDNVYKHEQLLSKIRSKKRLLDTLCEINVVEQVFNLGNSTVMQSAWKRGQNVQIHGWIYGIHDGYLRDLEIKGTCRETLDQSYRKAISKLEHEQSLVVSHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPW8</accession>
    <name>A0A8J4PPW8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_007287</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000380">
        <property type="protein sequence ID" value="KAF2071393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPW8"/>
    <dbReference type="OrthoDB" id="410267at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17353">
        <property type="entry name" value="MFS_OFA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF317">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="262"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="432"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="467"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="510"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="4">
        <location>
            <begin position="98"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2071393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="607" mass="68339" checksum="9D2A1A55F893C9AF" modified="2022-05-25" version="1">MTSIILGDHKNNNDNDNDGGISMDSSTNNLNNNYNSNSNNDIDNNKTIIKNNNKNTLDKKDSFFLKYIKHHYREQTLQRTEKQIENEKYLFFGLVPFSRWVLFPCAFLVQFCIGSFYGWSIFNKPIDSYIFDDPKKGMAPITYYITMAVYGVSLLFVGPWLERHGPRKGILVGGTFFSVGQFLAAVGIHTKQIAVVYVGYGLFCGIGNAFTYLAPVSSLQKWFPDHRGLASGFAVCGLSAGTIVFSQILLPMINHINLVNTFIALGCIFLTVFIIQAYFFRNPPPGYKPPAPKTRVVRSRSLTHSRIPRQISDQDQETRHLSDPDLSKMSIIDAITSKEFRLMYVMFFANGVSSNIISSRLSNMVQDLFAKDAKTAAMVVTVNGAFNLVGRLAFGFASEKIGRKQSFNVILTMMVLIFWILTRVITEHSYGGFVALIWLYSLAYGGGFGTVPAFLTDLFGPKNISPCHALILTAWNLAGVGGGFLFTGIYDALRRKGHTVDEPILYNANFYWLVSVIMIGWVVSFFIPVKIKERLYPAVPNQIFQTSLYGKILRIVRKKDGGNPFEIVSAEKEKEEWNEFLIQYHEKLLEENENNNNNTPAITTDSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQD4</accession>
    <name>A0A8J4PQD4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_006419</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000295">
        <property type="protein sequence ID" value="KAF2072265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQD4"/>
    <dbReference type="OrthoDB" id="46564at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025784">
        <property type="entry name" value="EFM7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF10">
        <property type="entry name" value="PROTEIN N-TERMINAL AND LYSINE N-METHYLTRANSFERASE EFM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51560">
        <property type="entry name" value="SAM_MT_NNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2072265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="279" mass="32358" checksum="2CDAE4FC6928C16D" modified="2022-05-25" version="1">MSSPPNNSIEIIEESLNIFEEPTSSEEEVEYSFHTYKRVEPIDDTCNPSIKDITVRLSQKHSLWAHLPWNAGIALSDYFEHNVDFKNKNDSQKKTLTIHHPSSVLELGAGAGFPSFIAALKGAKKVVLTDYPDEELISNMKYNVNNSLPQNLTINRVYGEEHLWGKQPEDLFKLLENPTTEKFDIIILSDLIFNHTSHERMLTSCQKCLSDNGVVYVTFSHHRPNRMEKDLYFFEDAVKYGFESEKFSERKMQAMFDNDFGSEEVRKTVHFYIMKFKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQU2</accession>
    <name>A0A8J4PQU2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006473</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000300">
        <property type="protein sequence ID" value="KAF2072207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQU2"/>
    <dbReference type="OrthoDB" id="18991at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="878" mass="101427" checksum="904390127500C442" modified="2022-05-25" version="1">MSTNHNNNNNNNNHNSNTSRTTSMNKSKLIQPNFIDLKTNYDYALLKQFYDELMVPNFPKEEELEPLENWVEMFNEQNIDNNSNESLPLFQDPFKSLIEGGVIKNEENTKFSKEVSFHIVLAINPNHDDQQQQQQQKRSKIMGGVVFEYYPSINCGCVTYLLVNTDYRGQGLAGVLLEQSQHQLMLDGKSRGHLGGCNVIFLETNSAEKIKKEDDVMDPAQRHIIFYKLGIRLLDFDYVQPPLSKESGKCRDLLLTCLLTNNIPKYYLGKEERYYLPSSLLKNFVQVFWDSCCSRLDMGDWERDVDYQRMMDQIDRRERIPLLDLPWERPWTLVDLCDSSSSIVSQKPMISKFYHEILVPNFSRNHLVDLEPLENWFNMLSGQQKKNSTEDFHLLVALRYPENDDSIQEPTICGGISFEYHKTTNCGLITYVLVGGGPNNRAERMSRALIQRAVDILEQNAIEFGHLAGCNAIFLETGIHDDEVVDESFESMDKIVEISFKQQFLSNMGWKKVDMDYFQPPLTIDKGPSKLNLTILLTPRIPQLDSRVSTKETHYLPSQLLINFLGSFWKSTCLKTGFWYEQNNLYRKMMDSLSRRKRIPLLGLPWKKPFVFVDMSKDFHEDLFNHFYNALPSTTGHRKTFDQLKESLQTLDKDQEQHVVLAISYLNQSFLPSILGAVVFSYFKKSNCGLVSDLLIKSMRTKDDNLLEQWDIAGELLEQAVEVINDSARKTSISGANAIFLESGIQDQDQEFIIDIHSSTNTLQISNKFVPVSYSTTRQNQGWLPLDFDYKPNDNNPRNFLCVYNSSRLPTNINNSTNVKQSYIPRDLLQNFLTNYWSSTALSFSNLKGDNQNYILKVIENMRLNDKILLLQSNKSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR39</accession>
    <name>A0A8J4PR39_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_006603</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOSIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000312">
        <property type="protein sequence ID" value="KAF2072083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR39"/>
    <dbReference type="OrthoDB" id="116827at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22063">
        <property type="entry name" value="WH2_Actobindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016818">
        <property type="entry name" value="NOSIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031790">
        <property type="entry name" value="Znf-NOSIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13063">
        <property type="entry name" value="ENOS INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13063:SF10">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04641">
        <property type="entry name" value="Rtf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15906">
        <property type="entry name" value="zf-NOSIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="116"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="270"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="141"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2072083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="345" mass="38100" checksum="00779B9CA5A62DEC" modified="2022-05-25" version="1">MSQNALLSEVAKGANLKSVETVDKSGPKIDSDVHIKKNEHGALLGEISKGANLTHVETVDKSAPKIDANTHVGQNQHGALLSEIKAKGDNHERSLLQYGTKKERLGKDSIKNFDCCVLCLNELRSPMSCTKGHLYCKECIYSNLLDQKKEIKRKEKEWEQQQAKLQADKEKIKEKEQQDIVEKFEKDTVSAIGDKTTTTTTTAAAGTMELKSFWIPSLTPDNREAVLDKPSTNTICTEGGHLLKLKQLITVQFTPIRAKDDSTNSDKYCCPICKKTLSNSSELRMLKRCGHVFCSCLSKFDSTGCYVCDKPYTKDTVISLQKGGTGYAGSGSKLEAQKYTPTAII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSC1</accession>
    <name>A0A8J4PSC1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Smr domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_005788</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000240">
        <property type="protein sequence ID" value="KAF2072893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSC1"/>
    <dbReference type="OrthoDB" id="3231855at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013899">
        <property type="entry name" value="DUF1771"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002625">
        <property type="entry name" value="Smr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036063">
        <property type="entry name" value="Smr_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053020">
        <property type="entry name" value="Smr_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47417:SF2">
        <property type="entry name" value="SMALL MUTS RELATED FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47417">
        <property type="entry name" value="SMR DOMAIN-CONTAINING PROTEIN YPL199C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08590">
        <property type="entry name" value="DUF1771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01713">
        <property type="entry name" value="Smr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01162">
        <property type="entry name" value="DUF1771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00463">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160443">
        <property type="entry name" value="SMR domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50828">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Smr" evidence="3">
        <location>
            <begin position="249"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="149"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="14"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="69"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2072893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="321" mass="34772" checksum="2BD95BA060E39556" modified="2022-05-25" version="1">MGNLIGKTKKGNKAPKAAKAPKASKASTPAPASAPAPAAQAKPQHIDNIAATSTAAAAPATQNQKQSVAPASAAPATTSSSKATTKTIDVDRELHKYVIGTKGVNVKQIKDSTNTEITIPTDSDTITVTGENSNDVDRAIEMIKKIKSEHKTQAQKDQDHKNLVDEHEKESQRTEELYKKNQAEVDKIAQKRQQLFDQAEKEYAAGNKDKARELREEAKAQTALMEEAQKKAARAVFNDKNSKLDKFTIDLHGLQTKDAMELLEERMNSIKGSGKLFTIITGAGNHSDKNGPKIKPLVHQSLKDKGISFEEVNNGSIQCTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSP5</accession>
    <name>A0A8J4PSP5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">PA14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_006414</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prespore-cell-inducing factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000295">
        <property type="protein sequence ID" value="KAF2072260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSP5"/>
    <dbReference type="OrthoDB" id="19087at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011874">
        <property type="entry name" value="Fibro_Slime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011658">
        <property type="entry name" value="PA14_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051154">
        <property type="entry name" value="Prespore-cell_inducing_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001673">
        <property type="entry name" value="S_mold_repeat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02148">
        <property type="entry name" value="Fibro_Slime"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31137:SF29">
        <property type="entry name" value="PROTEIN PSIA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31137">
        <property type="entry name" value="PROTEIN PSIB-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00526">
        <property type="entry name" value="Dicty_CTDC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07691">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00758">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035165410" description="PA14 domain-containing protein" evidence="6">
        <location>
            <begin position="22"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="640"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="PA14" evidence="8">
        <location>
            <begin position="108"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="479"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="678"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="678"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2072260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="706" mass="76735" checksum="8DF58516058BF3FF" modified="2022-05-25" version="1">MRSTILISLFIVLAFAHFIYSQDRIVSVSIFDQHPTKNSDFEPELGSLKTGLVKTKLPTSRVPELGASTNDLKTGRINNPELFPTWFNRDTTKNVQIPFDIPFKKVLDSETNTYIYQYKNNAFFPIDKQGWDVSSTYRSYTDDDGKYHNYHFCLKMNYKFSYKGYETFYFTGDDDVWVYINNTLAVDLGGLHSKESASIDLSTQASKTKLGITPDNTYNFDFFYCERHTTASNMEITTNFDLFCPKFDYCGTCLGNGCCKPDADCNDNNPCTVDICPDANDPTVTASNWKSKCTHEPKVCTSNSQCLIPSCQAGTGKCITTPLPPKDRSSECLFASSCSDKTGWNYGNACNQTCQTGACNADGTCAVTTDQDCATQLGLGPCYTYSCDKNGSGCVATPLCKQSTTNPCLINECKIVNDQATCVQTTLAPEKCNCDCTGQLDSCLLKRCDVTDGSCAPIKIDGIDDGNLCTVDSCDKNTGKITHEPIVCVGCTECVKGKCEPSDALCEDNNICTTSKCGANNTCVATPISCDDNNPCTVDTCDQFKGCIHTPITCADEGSCSVGYCDTADGVCKTKPRQCTPENPAFCLIYECDENAGCISYDKPCVAENSRCKQGYCDNSTMECKTKDYDPQPFICKTAAVVSTAVIAGVTVAGAVALGLAIFGGKKGYDYWKESRGANFSSSNSNPLYVQNPNGGENPLYAGASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSQ3</accession>
    <name>A0A8J4PSQ3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_005614</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000228">
        <property type="protein sequence ID" value="KAF2073090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSQ3"/>
    <dbReference type="OrthoDB" id="1093at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054179">
        <property type="entry name" value="PSD13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035298">
        <property type="entry name" value="PSMD13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10539">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10539:SF0">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22037">
        <property type="entry name" value="PSD13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="170"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2073090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="379" mass="43505" checksum="CBD4ED7769B75328" modified="2022-05-25" version="1">MKPYEYLESLKQTIPDLAPHIIAIRETYDNKLWHQLTQQLEQIVVQPQMMDKKELPNIYQYFIKDFETKLKPLSLVEICIPIARQYDNVEARKFIETISQKVKKDKSAYIFSLSFIATMFLHCQDLVECKKTIELAKDELQGITGLEPTVYSCFYLVCTDYYRAKNQAGEFYKNALMYLSYCKLENISQEIQSSLAFDLCIAALIGDSIYSFGDLIVNPILKSLDGSKNAWLISLLKAFNLGDIAQYEALIQQHRDIVSTIPALTNNKQRLGQKISILALLELAFRTPSDQRSISFQKISNTTKLPLTEIEHLLMKALSLELIKGTINQVDQIIHVTWVTPRILDFNQIASMNQRITEWASKTKTSLQLIENDTIDLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSQ4</accession>
    <name>A0A8J4PSQ4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 2 profile 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_009906</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor Fz/Smo family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000849">
        <property type="protein sequence ID" value="KAF2068771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSQ4"/>
    <dbReference type="OrthoDB" id="20424at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050949">
        <property type="entry name" value="GPCR_Fz/Smo-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31787:SF3">
        <property type="entry name" value="FRIZZLED AND SMOOTHENED-LIKE PROTEIN H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31787">
        <property type="entry name" value="G-PROTEIN-COUPLED RECEPTOR GPCR FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035252359" description="G-protein coupled receptors family 2 profile 2 domain-containing protein" evidence="11">
        <location>
            <begin position="21"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="234"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="352"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="394"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="441"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="500"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="12">
        <location>
            <begin position="234"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="569"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="569"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2068771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="589" mass="65916" checksum="8E1C834381D746A7" modified="2022-05-25" version="1">MKPIIQLLLVLSILIGLISSQIVYPVDKTAKCEPYIGDSTDIKCKGRLANEASIYVTGNKTQQSIMSELGLYFSFFKFSQCRDDPMALVTICSFGFQECVEYQDPITKETLAFPRRTCFDNCDAQIEKCGLKGVYDVCSLKEPNGLPSFPIQSSVYDLNSFGGPSNFSVECMNPNSIVIGSTEEVANDCPYPFTQVNSTDHAADFIKGYFFIGDSNCTSPCPPPLYKAKTWNQIYTMADVISCLALISTTFLIFTIGVLNKKKSKYDKKNLFFLVSNFFMALSGVMIAANGTKNTLCPEPNRFGASYDHVCVASAFMLHFFAIYAIEWWSIMSFELWWNIKFVNRKLDLDKYYIVISTVIALVLPIICVTNKQYHSGIGNTVCWIKKQEYQDGTFFAPLGICLSAGTVFIIMVLYEIYKIISRSAADHSWKTILSLQFKPFLNVVLFYTTFLYLFIFARYISANFSKFESTIGDYFICISTSPTPEECTIKGPSSASLGYFTYCLRIYGVYCFFIYGISSRNLQIWKDSWVCNNRAATFFGKKLSKITSMFSSRSSGATTSTVQTASGATGASQNFSSSVSVGIDDEHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT33</accession>
    <name>A0A8J4PT33_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005361</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000211">
        <property type="protein sequence ID" value="KAF2073343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT33"/>
    <dbReference type="OrthoDB" id="20932at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.980.20:FF:000011">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.980.20">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="413"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="97"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="414"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="428"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="439" mass="49045" checksum="8B51A0A9E2E1369A" modified="2022-05-25" version="1">MESHDEELVLQILFGLKNNNYNNVKNNNSAPASPCKKFRKEAQAPASPSLFGSSSQDIDFVPSSPSRGFLSYNSNKYEVPYSCESPTLLPPVPTLGSNRNLIPSPSNSLSNSTESLDSFTISSSPSQSVPSSPPFGKSLSLSSLKQQKFVSLKNNDTLSPSTSYTNLLSSSSPSVKIPSQSCNNNNNNNNRLLNIQCTLNECLLCQRGQPDVLVKSPTWASIMRVVFFTLHNEMKEKQFFSLKTDVYDFMTTHWDVLCLHKKRSDNWHKQIQDMLSHSKNIFESGMDKYKQNGFWRLKQAIDPWVLPQKGGRKSSSPSQSSPVLSLSASSPSILSSSPPSSSPSFYVPSISATTSPLILSNNNSCNIPNIKHKYHQSTTHLFDEGRKKSSIDNLLISSDSVEMVYNNNKKRTYQESQYNDNQRYSYSDDSEELYIDDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTX5</accession>
    <name>A0A8J4PTX5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_005183</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000200">
        <property type="protein sequence ID" value="KAF2073507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="19599at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031248">
        <property type="entry name" value="RNF213"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22605">
        <property type="entry name" value="RZ-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22605:SF1">
        <property type="entry name" value="RZ-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="309"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="1835"/>
            <end position="2085"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="2280"/>
            <end position="2425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="4334"/>
            <end position="4358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="4972"/>
            <end position="4992"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="4348"/>
            <end position="4358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="4972"/>
            <end position="4985"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2073507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="5076" mass="587972" checksum="7BCD6897F05C903B" modified="2022-05-25" version="1">[encoded data, 5076 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU33</accession>
    <name>A0A8J4PU33_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_005231</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000203">
        <property type="protein sequence ID" value="KAF2073446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU33"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00257">
        <property type="entry name" value="beta-trefoil_FSCN-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008999">
        <property type="entry name" value="Actin-crosslinking"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF184">
        <property type="entry name" value="GTP-BINDING PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50405">
        <property type="entry name" value="Actin-crosslinking proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="3">
        <location>
            <begin position="4"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2073446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="390" mass="44585" checksum="81458D898B5B2E60" modified="2022-05-25" version="1">MCERTILIIGKTGSGKSTIANVITSTNEFKEGEYGVSETKNFKCLNFIHNGVNYKVIDSVGLGDTKLSERQVLDQMAQACSAVTNGISQILYVFKGKLSQDEIDAFNLIKKVLFGQLNINAFITVIRTSFPKYRDPTSCQMDIDLMINESIQASQIIGGCNKLIHVNNMTVDEQPGLENRLDTNTRLLIHLMGCTLILNTTHSTMINNVNKYFYDKEQEIKRQQEREEQLRIAQIQREEYEAQILVQKKLKGYDYWNKTILIQPIALNSCFLDNQFLVFTPYGSKYQHIPSIAHIQFQMIPVDDGHKVAFKSLLNSMYLSATMGSLKFRDHISETEKFILIKQNDMWVILTHFGTYLSARPQMPLHPDNELIVLITAKCEMPEHFLFVSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU45</accession>
    <name>A0A8J4PU45_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">OBG-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_005061</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000192">
        <property type="protein sequence ID" value="KAF2073642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU45"/>
    <dbReference type="OrthoDB" id="545683at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04938">
        <property type="entry name" value="TGS_Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01899">
        <property type="entry name" value="Ygr210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.470:FF:000001">
        <property type="entry name" value="GTP-binding protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000037">
        <property type="entry name" value="YGR210C-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013646">
        <property type="entry name" value="YGR210-like_G4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF1">
        <property type="entry name" value="OBG-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08438">
        <property type="entry name" value="YGR210-like_G4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="3">
        <location>
            <begin position="5"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2073642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="424" mass="47689" checksum="E471A18F8485A1AB" modified="2022-05-25" version="1">MSHELVIGCVGKPSAGKSSFLNAATDSNAKVGNYPFTTIEPNYGITYYPTECPCKRFDKISVCSPRYGRCEQGTRFIPVKMLDVAGLVPGASEGLGLGNQFLDDLRSAHVLLHVVDVSGTTNEKGEETTGYDPINDIQWLKDEIHQWIYNNLWKRWGNIIRKQKATKVQADSILHKKLSGYGTRLFVVKEALERIDLKEPISLDGWDQDQLHKFVDSFLDVRFPTVLVLNKVDQSASDQNISRICSRYPDYAMIPTSALAECFLKKMRQQGYIQYKEGDDNFYTSDTVSTLKVCDEKVKGRLDKLQDLVLFRYGGTGVQLAIKTAVELKKFIPVYPVKNLKTLSCSIKGGDVLRDCMLVSKGTQVREFAGMLHPDLEKYYLYAEGINGQRLGESDEIQENNNIIKYTTAALESVGKDKDKDDDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUM8</accession>
    <name>A0A8J4PUM8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP synthase F1 complex delta/epsilon subunit N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_005719</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase epsilon chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000235">
        <property type="protein sequence ID" value="KAF2072979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUM8"/>
    <dbReference type="OrthoDB" id="270171at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12152">
        <property type="entry name" value="F1-ATPase_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.15.10">
        <property type="entry name" value="F0F1 ATP synthase delta/epsilon subunit, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00530">
        <property type="entry name" value="ATP_synth_epsil_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001469">
        <property type="entry name" value="ATP_synth_F1_dsu/esu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020546">
        <property type="entry name" value="ATP_synth_F1_dsu/esu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036771">
        <property type="entry name" value="ATPsynth_dsu/esu_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13822">
        <property type="entry name" value="ATP SYNTHASE DELTA/EPSILON CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13822:SF7">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT DELTA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02823">
        <property type="entry name" value="ATP-synt_DE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51344">
        <property type="entry name" value="Epsilon subunit of F1F0-ATP synthase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATP synthase F1 complex delta/epsilon subunit N-terminal" evidence="10">
        <location>
            <begin position="37"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2072979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="170" mass="18676" checksum="0F5D132C6D6A346D" modified="2022-05-25" version="1">MFRTVFTRSSTLLNNKTAFVASGKRFYADAVKSEDLLTFSLVLPHQTIYKDKKAPLITLPGAKGVFGVAKDHIPKIAELKPGVMQINHEDGKEEKFFLSGGFAFINPDNTCYVNAIEAFPIEQLDQEAVKKGLAQYTQAYNDATEETAKAVALIGLESYQQMAYACGVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUR4</accession>
    <name>A0A8J4PUR4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000198">
        <property type="protein sequence ID" value="KAF2073535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUR4"/>
    <dbReference type="OrthoDB" id="10551313at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021837">
        <property type="entry name" value="CfaA/B/C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38739">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38739:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11912">
        <property type="entry name" value="CfaA_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035169796" description="Secreted protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="24801" checksum="FCB373D29F2FAE24" modified="2022-05-25" version="1">MIKINNILLSLAVMVLLMQNYGEALTYVNMVPYLNPTCSGESSGLGYSWVVGQCLGFKGNNFVVSLDSHNVSTIAIYQGKDEQCSGAQQGSRTFSNGGCYQVNYQNTEQWYYNGWVKIEVVEDPTNTIIYGYRETVYASTDDHCQDDYQMYFYYTNNSVFKYSNDNYYVFYCQPDTYTPFEIDCENGPTSCFTTPNNSPCGKNYNNWWRNYQVQSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUV2</accession>
    <name>A0A8J4PUV2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosomal protein L7/L12 oligomerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004799</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000176">
        <property type="protein sequence ID" value="KAF2073911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUV2"/>
    <dbReference type="OrthoDB" id="250175at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000206">
        <property type="entry name" value="Ribosomal_bL12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036235">
        <property type="entry name" value="Ribosomal_bL12_oligo_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL12M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48300">
        <property type="entry name" value="Ribosomal protein L7/12, oligomerisation (N-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="151" mass="16353" checksum="0302B739B5E5D226" modified="2022-05-25" version="1">MFRSIKCLSTFSRLSQRQTTLYNGARTLCTSTGVEFKILTKEEKDAHDKKIEEFAERICELSTRDVIILTKAIQSKLGLPDIDLSNLGSSAAPAAGAPAAAAAAAPAAAKTEFSVKLIKVADGAKYKIIKELREIKPSLSLMEVSVLHIFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PV06</accession>
    <name>A0A8J4PV06_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_003252</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000100">
        <property type="protein sequence ID" value="KAF2075423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PV06"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051625">
        <property type="entry name" value="Signaling_Regulatory_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872">
        <property type="entry name" value="BTK-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="224"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="276"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="328"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="385"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="438"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="569"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="716"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2075423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="878" mass="98455" checksum="1D5F9A3E610E1AE3" modified="2022-05-25" version="1">MKGDFMKKIDPPMGANITKNDQKGKTGQQLSQAEKDILEYVHPFLYFKNNFYQFSSIADDDRFVQIFTAVDSEGETLLSVMIQTDFLRTIDSFLPRFKKFTQLINIVNSYGESPIHICALYNKVDAYSKFIALGLDIDYTIKDCRGLTSLDIALSLKHTTLSDSLRTKCIQTNSNIYHWGQTFSPISGLTTIQQPEALCFNDELSAPTQIKYGSNFGMCLTDTGSVYSWGLCSYGKLGHVKKIDHVLPTGITEFKNKFIKEIACGKDHSMALDDLSTLYVWGNGSGGRLGLGTSDVVQVPTVLKFFTEILVKSIACGQEHSMVLTQDGQVYSWGNGLMGKLGYQVIPGGFTSQLSQPTPRKVPHAAKIAQIACSNWHSVLLSVKGEVYSFGSNDKGRLGRQLYSKTSNCFEVRIVPIEQKIKKILCGLNFTVALSEQNTIWSWGSNAKSQIGLPYLGGVTEFFDRPTISKHLVSYSIQNVEVGNEHVVVLTNEGEVLTYGSNEFNQCGNGTVIEHGDFRITRVKIPDASVVFKISAGGNNSMISLSMDHQTFGLEMSKLLNNNTNVPFSDLRFIANGLYDRFTECNRAVVAARCPTLDTLIRKEIDSPNQGNKVALSLTRTVTSETINNVIYLSFPVEEFETLNVLIRYIYTDHANLIKAAKELGPLSLDLKLLRLRALWCFHNKENLLEMKSVPGSTLLEDFDNLKNEDFLHYYSDISFRCFNEDQPPEIVKSYKVFLGLQSTYFKTMLIGNFVEKDKRVIDLLETSPNAVSLLFHYLYSQAIPTSLNDCLELLVISDLHQLPRCKDLCASIIRTHIRPDNLCYIYNIALYVNVKSLIMLCESKLNSTPNVDKMIEFDTLPEAIQEKLKINCTTTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PV94</accession>
    <name>A0A8J4PV94_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_004511</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNase II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000161">
        <property type="protein sequence ID" value="KAF2074166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PV94"/>
    <dbReference type="OrthoDB" id="10261598at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004531">
        <property type="term" value="F:deoxyribonuclease II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004947">
        <property type="entry name" value="DNase_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10858">
        <property type="entry name" value="DEOXYRIBONUCLEASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10858:SF23">
        <property type="entry name" value="DEOXYRIBONUCLEASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03265">
        <property type="entry name" value="DNase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035175694" evidence="3">
        <location>
            <begin position="27"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2074166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="333" mass="38510" checksum="FD6693D6B03A32A9" modified="2022-05-25" version="1" fragment="single">MKEIQRRQVFVLVYLFLILCIGNVRGANEMCRADKILTGKDDEVDWWFITKVKGFGDVYLYVDSTMDNTATFKTGYYLRSVHSALGSTVSPFTGGSPDDKKNFISFCDYAASSNQATESFSKFYEAGAHQKGMIGWTETSNNEWKGFYIDHSMPYFPQIHSKTFMNGPINFDPEKITATLTQEQLSLTRMYFSYYGWRKPFFGRNLMPIDSGGCVKDYLLKHDQDRSDPIYRFKEKFIKPDQKESAEKEKIAQNERNLKGAVIKMDDIFPFAIVHTHKLKPSQHIFCISIDTKDKFLDFFKHYQYISNEGIVSNIHKVSDSSLSDIKPYISLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVI4</accession>
    <name>A0A8J4PVI4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">PAP-associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_005032</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000191">
        <property type="protein sequence ID" value="KAF2073647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVI4"/>
    <dbReference type="OrthoDB" id="20488at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002058">
        <property type="entry name" value="PAP_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271">
        <property type="entry name" value="POLY A POLYMERASE CID PAP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271:SF40">
        <property type="entry name" value="POLY(A) RNA POLYMERASE GLD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03828">
        <property type="entry name" value="PAP_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="10">
        <location>
            <begin position="77"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="PAP-associated" evidence="9">
        <location>
            <begin position="284"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="409"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="409"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="478"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2073647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="505" mass="58132" checksum="628252805474C6C7" modified="2022-05-25" version="1">MYNLTNNQYLESVLPILPEYLDIDRFFEKIPKSLRSTIYKYYSNFKNNLMNVVLSNMLASSAIKSTPQELINVNNQLFHYASTIRCSERSLKTKLIKSLESLLKIHFNKFDLQPYGSYLSELDVQGSDVDLCLLNKLPNTQVYKSHYYRLSAILEKNGFKVTHTIVHARVPIIKFNKENINFDIGFHNDIGIKNSLLIKAYSLLDPRSFALLTLVKKWAMSKHLVDAAGGYLSSYSYYNLVIFYCQTLVPSVLPALHNNPNLIDLVLKDQAHKLVYKSKNTMGLVSLFHGFFRFYSSFDFDQKLVSIRKGVISSKSTANLTLLENNTNASMFIEDPFDTTFNPAKTIKEENMDIFKMNLYSFEYFLRTQLQGPKVDEYFIPYFVCEDCFLDIPFPPLKDSKVEANNIISNKNDNTKSNNTTTSTTTAINSNKNNDNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNSKKIKQNKIKKRKSINKNKNGKPKQQQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVJ8</accession>
    <name>A0A8J4PVJ8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GH16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004227</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000145">
        <property type="protein sequence ID" value="KAF2074482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVJ8"/>
    <dbReference type="OrthoDB" id="20596at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035317792" description="GH16 domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="730" mass="80609" checksum="AD368BA4CDFA9AE4" modified="2022-05-25" version="1">MKRNLIFIFLIVLLLLNHTYCLDKAATTATTPATATTAPVATKAPAAVTTPPATATTAPITTKAPAATAAPTKAPTTTKAPTTTKAPAVVDESSSVESPISSTSESGSTSSDSSQIIIPCGTSFTDKCADGSSNFKALQTTEDNKPYSCSLNPNYVTFDESDSRSVMKLAVDAKGCPEDCHKLKYNCARVNSLDTDLTYGSYSCVMRLSKLSSVINVCAIENGGSGDGASEIYMKYQFPYFSIGYKNFGKEKTLIDPIKKDIDLNGYHNFTIAFRNTSITWYLNGLEMTQRGGSIPMPPMRFYAALLPDPTKDYNATLTAEAKIDTLSYLAACDQLYKYDNFGLRSIPPYYRENCTYDVEYTIFNDTLHEDWRALSDGYYKVPEERPQYQRDGPGVMAFEIRTETIPFNLLSKKYIPVRRHKYISFWLNGGDKGGQDIKLELFNTKYSTRGSVASISLSTFIQGGIRPSTWHKVIISMREFQVNPSNITTFNAFSFRGGHDEYMGAVYIDDMRFANSSACLADKLPLFKKGELLNGINGNYSSGAISFNSKTTMYRELVGKTMKNFTTIEFAVTPANTLNLSFFNGTVSSAEYYAFSISFYYTPKASYQNSDENIYTEEPTLSCQLCAQIGDIMPPCLGLSEYMGGKFPSGVWVKLTIPFTDLNVWDDAEISSLIIKTQYYDIQGYLSIGSIEVGKRADPPIPEDSFAMKSNSLNLSFIFSCTLLLLLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVK9</accession>
    <name>A0A8J4PVK9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005009</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000189">
        <property type="protein sequence ID" value="KAF2073692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVK9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041061">
        <property type="entry name" value="DAAD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18752">
        <property type="entry name" value="DAAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035182983" evidence="2">
        <location>
            <begin position="19"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="466"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="525"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="548" mass="63833" checksum="48750BD55974ECF8" modified="2022-05-25" version="1">MMKKVYFIFIFLIPITLSLWNEVPVDVIDQLDKMYDIIFPKHSKAPISQFGFAFNPSTLEAQSFEYPNSEWTILIDGDKKVVRIKGDQSKFREKLEKFDQDQFIIVQMMDDQKKAPYHSESIILEVMNTYEGLKSKCQNGFYLFTKFMPCTHCTQKLLLERQGQAQPTFRSERMSQLLKPFVPFTTTSKFYLLYSFQFDEDKLHYENLLKFQISNIFLVRYGYNSWKEGKEGDPKKWNPKSIQERALEEFKYKNSGIEKIGSLLNMFHDRIKNRKSRLILFFERILFPLCNKICGGDWDLVCWRTVMRSLETECYITNENSVLMMGFKSYLENEAKLYQDSMEKVLDYQGNKDKGVVDALISSKQRYRVPMGPLSSKDDIFIDEDGTVRFADITNKNWCGDDIESKKKQYTIFCQQPECQQYSVEYNKNANAIVAILVDDVIRDGSISTTTAEETAQVMAIISDSKEAPHSTIEKSPLLPKTKYNNKNNKQKKQNVYRNPDDNVSRKRKPLNDGAPLTQPTTAEKTNKTPDHKKQVPKRGLCPQCNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVM5</accession>
    <name>A0A8J4PVM5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_008218</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ubiquitin conjugation factor E4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="EMBL" id="AJWJ01000488">
        <property type="protein sequence ID" value="KAF2070466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVM5"/>
    <dbReference type="OrthoDB" id="20295at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034450">
        <property type="term" value="F:ubiquitin-ubiquitin ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16657">
        <property type="entry name" value="RING-Ubox_UBE4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000055">
        <property type="entry name" value="Ubiquitin conjugation factor e4 a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019474">
        <property type="entry name" value="Ub_conjug_fac_E4_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045132">
        <property type="entry name" value="UBE4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13931:SF2">
        <property type="entry name" value="UBIQUITIN CONJUGATION FACTOR E4 B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13931">
        <property type="entry name" value="UBIQUITINATION FACTOR E4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10408">
        <property type="entry name" value="Ufd2P_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="12">
        <location>
            <begin position="1022"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="83"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2070466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1110" mass="127124" checksum="E2601E3A3A7B5FE3" modified="2022-05-25" version="1">MKKMFLKFTGKEGDGGDPNPDKNDNSTTDEDMKDADQDHPTKDEEKEAKKAEEARLKRLERFNIAESEMVKLQEAELERQKQAAAASAALKASPPITSPTKSSPPLTSSKPPTTTTTTTTTTTTTNKPTITTTTTTKPTDPLNTVTQNKIRNCDIPMLETDYFIIERIFLVYLTPTNYPKCVYLDSLSQELKQESGGKTLQLSRPILDRVIVERLGIAVPGTNVVDYLICCFNRLKDFKRKKLGFTLDNQLLEEATDLILLYFGISLTVPDMFPSNFNTYGSGPLQIMAYLKQEEAEQITDEFIQQFIDHYSDDKVPIFEPLFKYLVTQMPKVSLIGEVIPFYRAFSKLVQFKHISDIAIKLPYFNPAEFNGNQMENSTILGTLFMPSSASDDRVVSQQYFQSASTLPPIKVNDAFISIRSVQKNIEATLHELLRGFLKVSNENKQAFLQWVTSAIEKNLGRTKMNVDRATVASDGFILNLSSVMVMLCESFIDPSFSKISMVDCNFLINGRHNISQDTRLCATSEEVSNWIQDKSIKPPVVPATFVTECFFITLRVLHIGISPCISKIKNIEYHLRDLESAKKTILDSKIKWINSPQARLFESQLELLTKKEDMIKGSLYALDAQLFELTFIQKTSNFLLFASTWMVKVANPKGLQLPLPMPPPLEFAALPEFCVEDIVDFFNVIITHGFQQALENIPLEPLMKFLITILSSPDYVKNPYLKAKIVEIISGFIPRNERRMGSERTFSLLLEGEQVRDNLVEALMRFYVYIEFTGGHNQFFEKFSYRYHSATIMKYLWTIPFYRQKFHDACKDADKFLKFYNMLINDSIYLLDESLAKLAEVKANQLLFENPNWDKDLTPEQRKEKLDSHNRFESIAKSSLSLANENINTMLYLSSELLEPFMRPELIDRISAMLNYFISQLVGPKCTDLKVKDPEKYNFDPKKLLDDITSIYINLGADQRFLQSVVRDGRSFKLTTFQTVEKILARERIKSDQDLEKFDKFINLLAKTEKEEQQTEEELGEIPDEFLDPIIFSLMTDPVILPASKTVIDRQTILRHLLSDSTDPFSRAHLTPDMLIDDHETKKKIDDWLASKKEKINRRSSVFFIKMCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVQ7</accession>
    <name>A0A8J4PVQ7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNase NYN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004619</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000166">
        <property type="protein sequence ID" value="KAF2074090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVQ7"/>
    <dbReference type="OrthoDB" id="392925at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036464">
        <property type="term" value="C:cytoplasmic ribonucleoprotein granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021869">
        <property type="entry name" value="RNase_Zc3h12_NYN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051101">
        <property type="entry name" value="ZC3H12/N4BP1_RNase_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12876:SF35">
        <property type="entry name" value="LD08718P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12876">
        <property type="entry name" value="N4BP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11977">
        <property type="entry name" value="RNase_Zc3h12a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase NYN" evidence="2">
        <location>
            <begin position="762"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="524"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="94"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="195"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="233"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="260"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="525"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="570"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="590"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="616"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="635"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="646"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="657"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="919" mass="101155" checksum="E5637F365B86A98B" modified="2022-05-25" version="1">MNLSDSDNDTLIASCFDFIDINDNYDTSDFDEDEENEDFLDPSYHSLNNIEQYFKANNSDADDSDEDVFKSSTNATGTHDFSSTPLRRRELLLQQQQQQQQQQQHQHQQQKQQVQQQRSSNNNINNTISSSLLKETTTTTTTTTKKEEKQQQPQPSTVVVDEPSSIDLHPPPKTSELYLEAAASLREKKKKNSKKAAAATTATTGNSSSSFKILKRFDDTAKGDVSTSGGSGATTTTTTTTSSSSTNGPRLGKKERAASKAKPSSSSAAGTTPSTTTTTTTKKSSTRSSSSSKKGQGNTSATGDSKESLIQVGRILGEVPIAYDLNTVSFSPANSFQKSEIVLVPRSRGGFTYARIFDTVTTTMCHHDTTVHHPVHQFRAVYPSGRNENLYKDLPSSYLGKLVPLNELPSNVKSFTIAQSTDGVPSIGQKATFRDLNSLVFSPSSKFQTNQIALIPRSRGGFTYGCILKECKVPCKVGDIKHEVSGYRVVVEKSSDHTIIKDLVVGNIGKLFFSVENVPNHKHFNPNNSSSVSNTNGLISNPNANGNGSNALASPSPSSSSSTTKKPSRRERAIEKEREREKKLHKQHQQKYSNANGSSGDKKKLAFDVQPADQVSSQSSSSSSSQSSSSSQSSLKNQEIQNGKNLLKEERNNNDDKDSDGDDESLEDEEEEEILWNPSAIGLNNIDSTPKAKVIPPIVSEEQTQVSSSPPAVVSSITPPAAVVVVDQPNQQIQQPVGKGILKDVPADSLVFRSNKKKTGKSYIVIDGPNVAKKYGTKKKFSVMGLKKALDYYDQRGYEVVAFVPESYASRKNHHDPKKNFTLFEYQDVADDLVAFQKLTESGKVSLTPAQDYDDIYTIHYARMHNACIVTNDRYNDIIDKCQESEKQKIRKFIRDHCISFTFVRDEFLPNPDFKFPTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVW5</accession>
    <name>A0A8J4PVW5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000077">
        <property type="protein sequence ID" value="KAF2075993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVW5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="385"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="387"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="449" mass="52702" checksum="8B55BDF064621749" modified="2022-05-25" version="1">MVESDQDILDRDLHTKWVSKDVWGNNLKPGDYIAIERRRGQVLGRITHVLNDKAPFRAYFEIKGGGGGFVAPGQKFKVGYRLFLEEKKKIRENQKAYEQDCIRDRVDKMMETAKDRAKKEPVVYEDPPQYYRDPQPYYYNPYSSGKKGSVVGITSSNRVREIPKFVERASDEVYPTPACNLFGLEILDQEEYEKRCQEKASLKLLKQIKKAELVELELKNHYNNTKKNNSSSKKKTFEINESRVKRLEKKHFKQLNIDLNYFNNSNSNSNNNNNTHSTNQSVFQYYDNNNNDSNNNDNINQYDYQYNDNNQYNDNNQYLDPETGVMFRECFNNSDDEYEDMDGGYTDNDQNYYYNDNSIDDYKIKSCLDDNEYDFGYQEPQQISYFSSSSSSSSSTTTNDASLNPTKLNYKNPELTQRLQSLIDLKDKSYISETEFNIRKEELMADYYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVY7</accession>
    <name>A0A8J4PVY7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">phosphatidylinositol N-acetylglucosaminyltransferase</fullName>
            <ecNumber evidence="2">2.4.1.198</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">GlcNAc-PI synthesis protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_004108</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.198" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000139">
        <property type="protein sequence ID" value="KAF2074601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVY7"/>
    <dbReference type="OrthoDB" id="734129at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000506">
        <property type="term" value="C:glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017176">
        <property type="term" value="F:phosphatidylinositol N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03796">
        <property type="entry name" value="GT4_PIG-A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000188">
        <property type="entry name" value="Phosphatidylinositol N-acetylglucosaminyltransferase gpi3 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000026">
        <property type="entry name" value="Phosphatidylinositol N-acetylglucosaminyltransferase subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039507">
        <property type="entry name" value="PIG-A/GPI3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013234">
        <property type="entry name" value="PIGA_GPI_anchor_biosynthesis"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45871">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45871:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08288">
        <property type="entry name" value="PIGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIGA GPI anchor biosynthesis" evidence="9">
        <location>
            <begin position="70"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="8">
        <location>
            <begin position="217"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2074601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="472" mass="53729" checksum="3393B8559510E887" modified="2022-05-25" version="1">MNTNEDEQQQHLQSQIGGNIGGGGTMKTKKYRIAMISDFFYPNMGGVEEHLYQLSQCLIKRGNKVIIITHTYGDRFGIRYVTNGLKVYYIPQAPFYNQSSFPTLYFTFPLFRKILIRERIDIVHCHQAFSTLAHESILHARTMGYSTCFTDHSLFGFADASSIHMNKLLKFTLSDISHVICVSNTSKENTVLRAQLDPHLVSVIPNAVDTTQFTPDPSKRDPSKITIVIMSRLVYRKGIDLIIDIIPNICKKFPNAHFVIGGDGPKRVSLEEMREKHQLHDRVELLGSVKHSNVRNVLVRGDIFLNSSLTEAFCIAIVEAASCGLYVVSTKVGGVPEVLPPHMISLAQPKSDDLEEKLTDAIIKLNGTPLEIHEEIRSMYDWNDVARRTETVYDIISRAPKVPFIERLRRFVGCGLWAGKLNCMVVALDTLLWRFLEWKSPRENIDEAIDFPMNEYLLYKNHINQINNNNNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW85</accession>
    <name>A0A8J4PW85_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Replication protein A subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_002488</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the replication factor A protein 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000069">
        <property type="protein sequence ID" value="KAF2076188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW85"/>
    <dbReference type="OrthoDB" id="31067at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04475">
        <property type="entry name" value="RPA1_DBD_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000041">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013955">
        <property type="entry name" value="Rep_factor-A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031657">
        <property type="entry name" value="REPA_OB_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08646">
        <property type="entry name" value="Rep_fac-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16900">
        <property type="entry name" value="REPA_OB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Replication protein A OB" evidence="8">
        <location>
            <begin position="323"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Replication factor A C-terminal" evidence="7">
        <location>
            <begin position="491"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="114"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="123"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="161"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2076188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="633" mass="70441" checksum="3A04AA9275B2F77C" modified="2022-05-25" version="1">MSNQPIYSCLTKYYEQAVNKINNEGVTTPQFFSDVKDVIFQVKLIKATKNQNGQPTQELFISDNLHYYPCIYHGGADELAVYKLENFQINQKKIIVIHNAELISKAPGLFGSPTLHPLSRKATSPNTSQTANTTSNSSSSNSGSYQQQKHTFNMPPSKPLNNFNNNNNNNNNNNNYNNGGSSIAGDNITPIAEITITSASDYNNTTIKAVAKNISRIISWSKGADSEGKLYNVVFSDASGEIKATGFSDSLNLHSRLQEGCTYYVKGARIKERNMTYNKINHPCELNLTNSTIVEQSDDSIQAPIHYSNLVSISNIANIATSRDKDKAVDVDVIAKVLSIGDIDMVKNNTLKKRSVTIIDDSNTTIDVTFWGESTEKLTFNVGDVLGFRKLKVSEYNNSKSLNFTFLSSVESENRQDLQHLNSNNANNLSARSAPSGASREDAPVVPISVINYDNNFHGEDSGEKKYFTLYGYLSKFDFKSEYDGDKKPNWYYDSCSKCKKSWASSCGCPGAESVGQWKISLRLSDNSGSTYVELLGETAKKFFGKTVDQMKTMLEESNKKASDVYLGNLYSYLYKVNVSVRLNKFNDTTRNVIMAQYISKASSQDFINLSKSYNNMSHLFESSVKARLPSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWD6</accession>
    <name>A0A8J4PWD6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pleckstrin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_005451</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000218">
        <property type="protein sequence ID" value="KAF2073224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWD6"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000003">
        <property type="entry name" value="Rho guanine nucleotide exchange factor 10 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053086">
        <property type="entry name" value="RhoGEF_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45834:SF3">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 3, ISOFORM L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45834">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="163"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="221"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="429"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="4">
        <location>
            <begin position="596"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="869"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="477"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="913"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="489"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="913"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="930"/>
            <end position="946"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2073224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="946" mass="107026" checksum="3BE12F41665360E5" modified="2022-05-25" version="1">MNNDEQDSSSSRIPMISVSNLSQLSLNGCQGSDDDSSVQNNNSISSNTSHRYKSILLNKNGSTRSVNNSGGSLNGSTSSPKKFSIKNIGASVTMANFTGLFSKQTSSKDNHHHHSSFSDFTGKVFNSKGVKKLRNISRRRRQRKKSKDYALRRKISQLMNSRWAMIVMVIATLFLLYIDDLMLATLAPDNTVSYAIISSLKILVFIIFAIDIIFSIYVQRLNYVLTLFFPIDLISIASLIPDIIAFIFGITSIAHAINALSLSRTARVARMASRISRMAKIINFFNVFMCADRVSTKNKHELQLHTEPSSIGKKLLGKTSHKVVVMCLLIVLITSLTLPSPDNTPYYKSILNLLSLSANQNGINSTNFQDTFTEIQSNSQTVDILYLKIHGDDIYHSQEDTSDWFSNYIIKVDQGQDEIWIDNQRNEQVWAALGITLTTCLLIILSFGNLLISRDFQILVIKPIERMVSLIKKISSMDRGHDSDYGGGDTEDNEFDDDTTNNDTEGDINMSDLENQYPSDTDYSDSIGGFDEEPETNIIVEILSDLAKKKIDNYTKEKNIIDGRNQFNSNLQSLISGIKSWCQRKQYLAEGKIYSKRARIVKEIFTTETTYVNSLKTAIDAFLYPLRTNMIVPLEELNIIFSNIEVLASFNQGFLDKLAEKVNNWSINQSIGDVFSHLDESGDIYSTYVNNYNDAIKTIESLKKDNEKFTEFLLETRESKAKGIDLVAYLIMPVQRCPRYVLLLEDLVKSTPSDHADFANIELAVKNLKKLTVQLNERKRDSENKLAIQDIYMKLVPPVQDILTPGNSVIIQSKLKLDSEKFYFFLFNTGFLKTEKHDNELLVKQYLSIRDNPVQVTSIADVPSMKIFNCFQIKIDDQSLVLFAKTPEKKTEWINAFTQFTKVQTQRTKSMMMMNSSSQLRRSSTSQNSKNSPQLSSSRLSVNSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWE0</accession>
    <name>A0A8J4PWE0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003716</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000120">
        <property type="protein sequence ID" value="KAF2074977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWE0"/>
    <dbReference type="OrthoDB" id="15421at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035144821" evidence="1">
        <location>
            <begin position="20"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="341" mass="37922" checksum="27A72476D0D1E150" modified="2022-05-25" version="1">MKYILIIYLAILCVKWSNSQFTSTGFPIKVLSDTSPWNTVIGPSPTYDSYSAAMMTRISSILTDNNISPVLGFAYYQWTAPIHLIDSSLSTVNRIVIYQPENTGYHETVDPLNTQRIENLPVSSTFNPDPMADGHMIVFDTSLKEFHEFSRFKWINSTYASATRYDRFAYNAIGTYPAYVPGTRWWMKSVRGAGAPFIAGLIRWDEMVRGDLNHALAFSGPVNRIKALSTSPWTTELCTPVAARSDGYALGPDTIMEGQRLQLNPSLDISSLPPKAKLIAQALKTYGGYMVDNAHGFNIYFEYKGPYATSSWNTYASDIGTLAGIPLSQFRVISCSTIATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXP9</accession>
    <name>A0A8J4PXP9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">S1 motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_001845</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the SPT6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000046">
        <property type="protein sequence ID" value="KAF2076878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="995477at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140673">
        <property type="term" value="P:transcription elongation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09928">
        <property type="entry name" value="SH2_Cterm_SPT6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09918">
        <property type="entry name" value="SH2_Nterm_SPT6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.650">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2740">
        <property type="entry name" value="Spt6, Death-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.850">
        <property type="entry name" value="Spt6, helix-hairpin-helix domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3500.10">
        <property type="entry name" value="Tex N-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.140">
        <property type="entry name" value="YqgF/RNase H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049540">
        <property type="entry name" value="Spt6-like_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042066">
        <property type="entry name" value="Spt6_death-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032706">
        <property type="entry name" value="Spt6_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028088">
        <property type="entry name" value="Spt6_HTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035420">
        <property type="entry name" value="Spt6_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035018">
        <property type="entry name" value="Spt6_SH2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035019">
        <property type="entry name" value="Spt6_SH2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028231">
        <property type="entry name" value="Spt6_YqgF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055179">
        <property type="entry name" value="Tex-like_central_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023323">
        <property type="entry name" value="Tex-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023319">
        <property type="entry name" value="Tex-like_HTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017072">
        <property type="entry name" value="TF_Spt6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037027">
        <property type="entry name" value="YqgF/RNaseH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10145">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10145:SF6">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14635">
        <property type="entry name" value="HHH_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14641">
        <property type="entry name" value="HTH_44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14633">
        <property type="entry name" value="SH2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21710">
        <property type="entry name" value="Spt6_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22706">
        <property type="entry name" value="Tex_central_region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14639">
        <property type="entry name" value="YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036947">
        <property type="entry name" value="Spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158832">
        <property type="entry name" value="Tex N-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="S1 motif" evidence="9">
        <location>
            <begin position="1130"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="437"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="703"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1441"/>
            <end position="1704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="73"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="99"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="133"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="151"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="191"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="203"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="444"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="730"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1443"/>
            <end position="1491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1492"/>
            <end position="1505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1506"/>
            <end position="1521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1536"/>
            <end position="1549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1550"/>
            <end position="1565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1566"/>
            <end position="1602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1630"/>
            <end position="1642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1643"/>
            <end position="1657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR036947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2076878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1704" mass="196294" checksum="86DBF88977A465A1" modified="2022-05-25" version="1">MKDNYSDEEGDHPLHPKKKLRNLAAANKKNRGDDDEDDIFDAPTLDGSNRPAESSGKKKNNSKHRKNRNHSDDEQEFVDSSEEDEDEPNEYQYDGFLVDGDEDEEEAEQSSKKKRSSRERRSDGEDNGEGDNQEGGSGGSGGEEGSGGSGSEEEEEEEEDPELANQRRQERRRKREEKKKKKQRLHRLKKIKYDDKGRSSADEFSDQEEIEGEDDMDNFVVDQPKRGYGGGGMENREMFSSIFGDDEEEMEEDEDGDMVMETEKSKDRLALIREQYEPSLLEEKHFTDADEQIRTKNIPERFQTRKGTVYVDERTTLDEAEWIYETSFNKKEAKDMRAIDAIVHVLKAIQKDFLEVPYIYTYDKDFFEEFFNLQDLWTIFDLDEKWNHIKISKKNLEQISSNNRLLDNYQHILNDCKSEENISDLYDLFQMMNGVSNSDKGHANNGNDDSNNNNNELGEDSTPKVRQKRAIKRDLYTVYHKARIGKFLPQFGMSAQEFGQNLMENYLGNTQKDPTEEPSTAALAYICLEAGDTNQVLQAARYMMAQEMGFDPFVRHSVRMIYKRYAHITTTPTLKGYKEIDAFHPFFTVKSIFEKPAHLFNDTQYLLILKAEKEGFIKSTMGISEKIHDTIIIPEMENLYLSDGTSAITQQWNEQRKKIIREALTRFLYPILEKELRNKLLTEASNRVAFECAQKLEEKIRVGPWRPKSESSRNGGNGKKRGNGGGQSWESDEEEEEEEYEDDEEEEGQQSKPFKIFSMCWGADKVPTMGVVLDINGEVINHVKLDFLCDRLGESLKDKKEKDVQKLRTMFIEHQPRLILISASEMDSKRLYEEMTEQVNRFKQDGIINKSIAIEYSDPEIGLGLQNSARYAEEFKEYPTVLKHAIAIGRCVLDPIVEYASLCNDSNEILYLKLHYLQDMIGKDFLLKLLHRCFINIVNAIGVDINRMIKYRFTSPTLQFVSGLGSRKAQMLLNLILRRGGYVSSRASIAKLLDQNVVYKNCIGFIQIKEQYLSESDADRLDETRIHPEGYEISYRIAAEALDKPHNADELHEYIYETMRKPKKLDRLDFDAFSDILEKHNGHSVKKMLALIKRELTNPFADIRSPYRPPNFAQIFQWLTGETDQTLRPGTLISATTIRNFEHNVKCRLDNGLEGTIPSDNISDNNDVKSLPRGLTLNCRVINVDKERFGVTLSCKESDLSPKRWEEKIFYDLTSNGQNAYLKLDDNPPSLAKLQQSEKKKPVKKERKVKRTVIHPLWHSFTCLEAENYLRDKQIGEPILRPSSKGFDHITVTFKFWDDIILHHDIKEADKPNAVSLGKSFYMGDVKFDSLDEILARHVEYLINNLNEIRNHQYWKNGTKSEIDEMIKQEKLKNPKRLPYYFGIDHQHPGFIILYHVPTSTPRHEPILIKSDGYVMRKQNFPTLNDLIKFFKQNYAQLLQNKPSSNSTGSGSGSSSGNNNQQSFNNNNQQRPQQPPQQQQSSFNNNNRSGGYQPQTPSRGSFQNGPQSSYQPKPQQPYQTPNRNPIQSYNNGMGGNNNNNNNNNGMMGNYQQQPKPYQTPNRPSYPNQGGNYQQPPPQQQQFNQGFGGSQQNNWNQPQQGGNYPPPPQQQYGGNFDNRGGNRPPMAPWEDNQQSNYHPQQQRGPPPPMYNNQPPPPQYGGFDNRGGSMPQQNPPYGQPNFNRGPPPPNNQNKPPMAPWEDSQWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXR5</accession>
    <name>A0A8J4PXR5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000091">
        <property type="protein sequence ID" value="KAF2075640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXR5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="22"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="600" mass="70923" checksum="5CC92F4EAB67073F" modified="2022-05-25" version="1">MNSNNYHNTNNNNKSYIFNNQIILNILGYCGPSDLFNTQVVSKVWYKLSRSNNVWEHALRNRIGDYTPIGTTVDDQVLEYDYYLKLGKMLFPQDAYHSYSHKLIFNLYPLLCPSNDQNLKNIDNAYGNDIQQKIKNQVEENYKFYRKSLDNLFKFMKYKPILDQDTNIISIIKDSSDFSRSSLSLNQGSLDILLHKHQLAFLIPRNPEESNHLTKIILIDFIGNEYGTQNNSSESKICISETKVERTFLDFDNRIDKHNHHQDKQELIHYWHRYHDGYVAEMGNSDPVGDIRGQDYYVSKMKLDKFAEFLDGAPLFGKSNQELYELLSLSTNHPKQMNNFEFSNTLDHCLVHHLQDIQFYFEYSEKNPEQIEFILFNFEIKSMMVKELIDRSDDNDIQQHEQQLFKAINNMTFYKYNCKFAQNNSSSLMNFYFSTQFSIPLSGNETVYFNIYFTLQDQNPNPDLINNNNNSIHHTNQHQYFISQIDFIIFDNQTEISLKRDIEFKSMYNKKPENGHLPKKNESFINGIQESFKINFPNLNFNIIDILKLLNLDKHIELKYNENSCLEIKNNINYNNEMTKDAKSFLKDYNLNTLLEKQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PY63</accession>
    <name>A0A8J4PY63_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1990 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002600</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000073">
        <property type="protein sequence ID" value="KAF2076086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PY63"/>
    <dbReference type="OrthoDB" id="46304at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018960">
        <property type="entry name" value="DUF1990"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34202">
        <property type="entry name" value="UPF0548 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34202:SF1">
        <property type="entry name" value="UPF0548 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09348">
        <property type="entry name" value="DUF1990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1990" evidence="1">
        <location>
            <begin position="45"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="216" mass="25449" checksum="F32DD8321048CC54" modified="2022-05-25" version="1">MMCLRKPSDSDIRQYLRDRKEEPFLYKNVYGTRDYPNKLDYEQDPKYKQFDIDQIRIKLGTGQECFDKAVKALKEWKHFDLDWVEFCFKDIPILVGETVGILSKQMGFWILSFCRINYVYDGPQEDGTVKFGYSYGTLKEHVEKGEERFIIDWLREPDAPLDSGDVYFEMLSFSEPQHWMSQLGYPVARYFQNKFTLGSVNSMLKSVGSSNLVKDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYG5</accession>
    <name>A0A8J4PYG5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_002396</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000066">
        <property type="protein sequence ID" value="KAF2076281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYG5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2076281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1010" mass="117753" checksum="8AAD284CBB033CCB" modified="2022-05-25" version="1">MIGLNLLRTQSKSTKKILFLTSRVLNTRSVGSNAFRSFSTLHQQEPQQPLSTTRKTNTTTTTITSKKGIFRSQIEQQIQPLEIIDGAVIPIPELPTTLSDSDIITFRALIGKNDSVFVACLEKICNIADPKMSYAKLNMFLSKIQADKKVEHLLKTYYFFQSLSKKPTGYAYESIFLIKECLIHDQPALAKAFYCLSKTLKTYPQFHLILDYYLEKKDFKAAGVFFQEFHILYPKLWVDQRTPNYLSILAEYMDVKQVMIDSLVEYDSRDIPESDMSQLIKNCFNINNFSHDLIATIVEGMAEGRDVGSNIYRNVIKRFIIAGDTKNTLRVFSSTYSKVNLGYSVFQALYTFLPLDHNPKALSGFINAFLDSMTVIDPYPASIELSVAIIYNQLKSSQFKESNPALAEKSATILNSVKEGLRHFPSSYVKNTIYNIVTFYLHHDQYDLALQWFSDSLLEYRLSHSIELFSCFLFYHKCREYQFVYTDRYPLATIKPSPNDSYVTTNAQRKSKRKPETVEEMAQKEKEIKDKYYHNQLFKFWQQHSETLIPDRKPILKLLEQYQNLDVSLKLRFVENFFKHHETPIDHLLPNTPLNTLNATDKELTLMLKHNDHNLIIDYLNANYFSKNIIPQGSLLIECAVLLKSSHSYVKFYDSVPTFVKVLLVNPYVEEASKLNFGDKIMKVTPLDSQLRPRNEHLYHMCFSYAINNNMVPLVYDILHSILYCQIQHWMHPLWKEVLEEWPLPEWKELSTLVLQYSKAYGTNKLFSSLYMTNYYIRIKLESGQYSECLSFIKALSRGSRDEKTMEYTIKLYRELFPLENSEKSKVNIKEWQTIKKEHFLLDVDLTPTYYQHLFDTLLAHRQENQEFILEYINGKFNEIVSSINESTLYSLLNHFTQLNNTDMVNRLFIEYTKQTKGGWPKEKKSFVLGKVIELMKVYNNNNNNETIQELLSKQQGPFEAEEVVVKPKRTYESGHLIKSGIEGKIRMILDHNNHFQNEIRLGDNLSSDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYK3</accession>
    <name>A0A8J4PYK3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_002883</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000085">
        <property type="protein sequence ID" value="KAF2075800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6019893at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032483">
        <property type="term" value="P:regulation of Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005112">
        <property type="entry name" value="dDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051696">
        <property type="entry name" value="DENN_Domain_GEFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005113">
        <property type="entry name" value="uDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296:SF21">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03456">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00801">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00800">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50993">
        <property type="entry name" value="Peptidase/esterase 'gauge' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="UDENN" evidence="5">
        <location>
            <begin position="19"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1054"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1096"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1141"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1181"/>
            <end position="1222"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1237"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1263"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1305"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1345"/>
            <end position="1387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="447"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="604"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="997"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1432"/>
            <end position="1621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="612"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1001"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1436"/>
            <end position="1457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1473"/>
            <end position="1490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1505"/>
            <end position="1530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1537"/>
            <end position="1557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1558"/>
            <end position="1586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1600"/>
            <end position="1621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2075800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1621" mass="180509" checksum="6DDEAF6B3B05426D" modified="2022-05-25" version="1">MTTEANAVEKYNSYHRLVDYFIVCGLNKSVVVGDIDKTYQVEILDRYPQTDYDDAPFPTHIWMFCFPAGLTLKPVAPEPSISMFCLTEFDGARFYASSLVYYKEIPKSHIESDHSTLYKPISITLLSRYPFYKTLKELLCYIYTYSLKSNLCISNKSSNSHDNNSSINFLNIERIISNIVNDLPRPIEGYRMVVDLPYLSSSPHINNINYSKSIQTNNLPDLDIPFHLLFHLLGLRNAIKLFSFALHERKILFLSSNSSLLTVACETLTYLLYPFVWHHVYIPILPDLLIDFLQAPTPFIVGMIQHPSKPKIDYQDIVVVDLDNQELNCSNLISNVPECESLKLENELRRILYPDLDTLSTAFPVHRDDGFSMNQQIRSAFLAFFVSFFYDIDEYRYLLRKYPTPITTFNKSSFLMKHSDPSIVTFLSELIETSGVTSFFDNAHSNSNNNSSNNNNSNLNNSTGLSNSNNSIGSNTNNTNNNNTNNNNNSSNQFFDLICKYHNSNINRFSSTYESVRHILKKHSNSTIYKTVQIPSPDSSELNLLVTLVPQHTLSFPDLNHCYYSTRTEYSKKFINTVPNNNSHSHDKKEGIENLLPDLTLTPTSVSPSKRSSIVMSTPSTPTTTSSNPIPMSIVDETGSLLPILSEQQLQDQELLKEFIEKYIELIFSPDDEGSAAGTTGAIDTEQQVLLLDILKLKNARQYFVESLTNQCPAASQKEALPDSTPNPRSSLGNIYGKKILCLETNWKLTLLVEIMKRVFSECNHSADFKTASLILKKSQEIYFKTDSGHLEHLYILFSQFKLWKNFYFWESFFFEGLVQLRREQLPASFNNLSLDWDTMTESVQNNCIQQEEDVIFKSLSTYAHLMIRLKIPMPDALRFIGRMVIMTNLSDDQYNTLTSLVEKIYQVDSINSNTGTTSVEINENGIIVSTNTSVDDINLGSVTNGEEGVSSANRDVPRKDSVIPNAQYFSTSVKRNIEIEKNGGKQYYQKLIDIKRKKGSNSSSGSHQHQGGTMRKSSSSNTVSTEASPNSSFVFNEPEVSSEKRDGYNVTTLKGSGSALTCLGIHEKRSMIVSGTSAGHILMWNYSDGRFIQRLTNHKSTVNCLGLDQKVLDTFCSGSKDKTLRIWNYNNSEWGCYTTLQEHTGEINCLDMKRNIIVTGSQDQSMIMWDARQNRLVHKFTGHSGNIISTLILDKKDMAVTTSSDNTTRIWDLKTHKTLHVLQEHHDWVTKAIAGGKYLFTGGFDCIIKVWDIATGKSVKTLSGHAGGINCLSYDEDKNVLISGSGDGFLKAWDVSTGYSIKSFKGHKDEVLAVVYRGETMISSSQDQTIRVWDVSTGVCHKVLRGHTDWVKSLSNISSPVNKFVSASWDSTVKIWDIESTNALRESSGSVNSQLLNQSNTNLALKATSTTPNEGSFLNFKFGSKSSLNLSQHGSLKSSNDSVSLKSSTGSAGSSAEKLHSPPSPIHSSLPNTSTLFQSKSPSTSSSGDNIVFKPFSQSRKDGTSAPTSPTTSTTTIIGTKTNNNNNNSNGGGDSVESSFHSGSSIGNQSDGTPVGSSLSSSFSSSLGRSSFSSNSSFSSSSPSSWRIGSPTRNKKSSSENTSTTNTTNNNSNNSNNNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYX2</accession>
    <name>A0A8J4PYX2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_002150</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000057">
        <property type="protein sequence ID" value="KAF2076536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYX2"/>
    <dbReference type="OrthoDB" id="14497at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="17"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2076536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="247" mass="28691" checksum="036BC9E383377000" modified="2022-05-25" version="1">MSNDKVNMTSNDEDTKKQILYNEISEKLQQLSELNDRLKEQNLQLLRQKEQNESIIDSIKQESESKSIQISLYESKVKEYKLKLKLKDEESKKLGFLNEELTEWKDTHRITDENYLRLKEMLRLKDDAINSLSIQLEQAMNKNSLLSESLKEAGDRGILFERDESFNINSASKQLLDYQKVLAYHKIKAVEDKYATIKNINLGLQKAIQELSVENEKYKYTLETIIKNNNNDSSNSNSNNNGNTGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ57</accession>
    <name>A0A8J4PZ57_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_006722</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AVL9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000322">
        <property type="protein sequence ID" value="KAF2071966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ57"/>
    <dbReference type="OrthoDB" id="19579at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018307">
        <property type="entry name" value="ABL9/DENND6_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051731">
        <property type="entry name" value="DENND11/AVL9_GEFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31017:SF1">
        <property type="entry name" value="LATE SECRETORY PATHWAY PROTEIN AVL9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31017">
        <property type="entry name" value="LATE SECRETORY PATHWAY PROTEIN AVL9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09794">
        <property type="entry name" value="Avl9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="3">
        <location>
            <begin position="6"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="571"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="571"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2071966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="739" mass="83637" checksum="21186756DFEE06B7" modified="2022-05-25" version="1">MTQLLEYICVVGFHHQLGNKIDFVYPHVEDIYNIDNTSNTSGTNLSKSTDKTTTTTTTTPTEAKYDWNLLPFYALPDGCHNSKEDYCFFVLEEVNPTTIPSQKLYGISYFKQKATKDLPAQALTPDDTRSTVQKALCILSKFPIFYSFKTQLSSATICYFNQGDFKNTDLLVNWFNTQNKEFMAKNKQRQMESNMYQEDQEILELLEMADKAPGTGEVLENIDLLNQKPNKVPIESDFFFGSKSTAKFIMHFGRNSLVMFKLLMLERGIIVFGPQPINAVSETIMSLVSLFPRSFDLLINQNASVNQSFKIDWSATEDVAVSQLALPLQIFSPKGTFLQPYLPLQQVNCLMPPGESYDNVFSPPLISGNPSAGSVAKQPGYLVGTSNNMFLKAPPPSTHAIVDIQSGDILYRDESLHKIMELTSQDRRFIDNICDKVELFYKSPTSSAYEGSEAWIHNQFENYIVCLLSTLCKYLKIEDGKTTITSLDALSPYNEHWIKRWAYTKNFYIWRNQMFRNPPLPNLSYPVHPSGPGDILDKFQDKFTTSMTQLEPLTHSLTSIWSGASSLFKLNNNNNNNNNNNNNSSTTKSSKHHSSEYKEDKYYIQEESDDSNGNNNSKWNDVKVTSIQAANKAAAAVKPFVSSSKVYARQFSSQLLNYLAPPPESIDEEIKEYGFQFMDDELLNSNINNINISSNSITTSTTSTTHTTTNTMKKKSYALSTSNTQIIDQNDEDDDDYEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZE4</accession>
    <name>A0A8J4PZE4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002882</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000085">
        <property type="protein sequence ID" value="KAF2075799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZE4"/>
    <dbReference type="OrthoDB" id="20482at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="108" mass="12643" checksum="BD41CCFEA2DBC4F1" modified="2022-05-25" version="1">MVKPSSRSNTPKKQPKEKEDTSSNMDEDVKSDKVEIDSQTLKKNKQFNAFISTDRPKDIVKKKKLTPKQMKKKTKGIENAINRVEKDDVFIEKKMKKAQTKKRWNALY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZK8</accession>
    <name>A0A8J4PZK8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Exportin-1/Importin-beta-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_006543</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the exportin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000307">
        <property type="protein sequence ID" value="KAF2072146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZK8"/>
    <dbReference type="OrthoDB" id="10261013at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040016">
        <property type="entry name" value="XPO6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21452">
        <property type="entry name" value="EXPORTIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21452:SF4">
        <property type="entry name" value="EXPORTIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exportin-1/Importin-beta-like" evidence="8">
        <location>
            <begin position="110"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2072146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1052" mass="121366" checksum="174C18C1EC72775A" modified="2022-05-25" version="1">MNNSNNSMDMENKTKELEILLYQFFTLDPNNSKRTEIDSYLSNYRKQKNSYEHVKYYLTHSTNEHVIWFSMSILEDKVNKSWDTVNPQERTGYVNLLHEIYFNNTKSTSAYIVTKVGQVLADMERFEFLCNPQSSQPYILNMTNLVKNQATSLRAINLLQYISEEFLTTKNVILQEKKIQLKKILSEQVPTIINTLTDYLNQLFNQNAEKKFKHGNLFQNVSFQVGSPDTNTYTGSFSNESKLITKAIFDALLSYFSWISLSELLTPSLLDILFKYLRLDTNNIPALECLNEILSKNCVPKEFEDFLMRIFHQIFSLLTDITANRGANIHQYNPAFLVKFTQFIALFVTNHLKRVESNPNFRISDFLSLLFQYSFLQTNSESFLHCLGIWTTFLDYLILQVNDKGITPPPKYTDGLILFQSELVKRTLFSFSKDILSDIDDMDNLLDDDAEEDNDEELIDIYKRKCIGTVSKVAELYPNASLENLYPLFTQNVTSFFQKIEENIKQGINIETGDDMNHLVSDVITILSLFGRLAEQFVVCFAPTFNAANFVFLQLLDMCSFTTSHNTFKYAPVWEKLQVQLLQSIRSFSYWLLEYGNQVRTVPNQQPEFDSHILKIINVIGPLFKSDIPEEISQAAGQLLMSLVIVSKPLNLFTLSETLVTNIHQICNPLSSKDQSIIYSAISCTILNPPSSVNLSHQWDVRRPKYSPFIKGITAYYLEIPQIPGFVENKIFCQDDMIERVQKILGILTSIIKTAPEVTTAKGILHDAISDTLPVTLGLFKVYVCYPKVLGSILNFFFALFEFLKTQVGLQFTQQTINTFLEVLGGDNLKQLVAESNVTGTAIIKKLIEILTFVVQTSGHNFESLLSNTIDFSMEKLYPIIKDSISPIKPSFFTLLYSILDCQWKHCQSEQYNKILSAFQNSFQQNDVNLFKQNLDSFEKLNKKLNFYDKISLMEPVFGCSFITIFFDLLISNPQGIHTEDIINTIYHFASINFDKFFNEFFNNYLMSKQNITNEQKINLRSNVSPDQDQPTFNRNMLRFINDFAYYTYINN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZP6</accession>
    <name>A0A8J4PZP6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="3">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_003467</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="3"/>
    <dbReference type="EMBL" id="AJWJ01000108">
        <property type="protein sequence ID" value="KAF2075249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZP6"/>
    <dbReference type="OrthoDB" id="407558at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000029">
        <property type="entry name" value="peptidyl-prolyl cis-trans isomerase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000009">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF496">
        <property type="entry name" value="TETRATRICOPEPTIDE-LIKE HELICAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0697">Rotamase</keyword>
    <keyword evidence="6 9" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="10">
        <location>
            <begin position="14"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="260"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="294"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2075249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="359" mass="40218" checksum="F74C5C2215731230" modified="2022-05-25" version="1">MTTELLSPENKRVYFDISIGDEKVGRIVMELFFNKTPKTAENFRALCTGEKGVSESGKALTYKGSKFHRVIKEFMIQGGDFTNGNGTGGISIYGEKFEDENFLEKHTRAGLLSMANAGPGTNGSQFFITTTQTPHLDNKHVVFGRVIKGMSVVREIENTKTGENDKPVKDCTIVDCGELKEGEDDGIAPRPVNDIYEDFPADETKLNGDAQHLKAGEEIKNTGNDFFKKSDFNTAIQFYKKALRYLDCCSKIDGLVDTQFTCYNNMSLCYLKINKANEALEACKQALSIKPDDAKALFRRAKAKALLKEYDDAIADFKLVLEKDPSNKEASVEIQKVEKVLREYNQKQSKAFSKMFEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZT7</accession>
    <name>A0A8J4PZT7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Serine/threonine-protein kinase RIO2</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Serine/threonine-protein kinase rio2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">CYY_001678</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000041">
        <property type="protein sequence ID" value="KAF2077046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZT7"/>
    <dbReference type="OrthoDB" id="10258631at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05144">
        <property type="entry name" value="RIO2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000053">
        <property type="entry name" value="Serine/threonine-protein kinase RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000307">
        <property type="entry name" value="Serine/threonine-protein kinase RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000052">
        <property type="entry name" value="Serine/threonine-protein kinase RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030484">
        <property type="entry name" value="Rio2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015285">
        <property type="entry name" value="RIO2_wHTH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018935">
        <property type="entry name" value="RIO_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45852">
        <property type="entry name" value="SER/THR-PROTEIN KINASE RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45852:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09202">
        <property type="entry name" value="Rio2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01245">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RIO kinase" evidence="20">
        <location>
            <begin position="64"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="318"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="430"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="319"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="363"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="432"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00068353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00068837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAF2077046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="503" mass="58984" checksum="1C936DFBF9DDE22E" modified="2022-05-25" version="1">MKLDPKALRYLSKDDFRTLVAVEMGMKNHEFVPVSLICNIANLKFGGGKKAIQNLHKFKLLFHDARKYDGYKLTYLGYDFLALKTLVSRDVISYVGNQIGVGKESDIYVVANDNNEEFVLKLHRLGRTSFKTIKNNRDYLKHRKSASWLYLSRLSALKEYAYMKALYDNGFPVPQPIDYNRHCIVMSRVRGYPMTQIMELRHPSKVYSDLMNLIVRLASYGLIHGDFNEFNILINDEEELTLIDFPQMVSTSHLNAEMYFDRDVQCIRTYFEKRFNFIGEKYPKFSDAVKQYDLDVQVNASGFTREFEKDFETLNQEQNELDFSSDDDNNDSDSEDEEEVEGQEKEIVDEKEINNDQDKEQESSQEEDEDEEDEDQEEESSSEEEKEENVEQVEEQFKDLAVNKNREYKFSKDNDFKVVLRKEYMDEMAQLDGERIQNNDTDGTKETPEEMRKRKEEEKKKMIEQKVKRQLGKTIGGRPKASVRNSVKNRSKKEISSHISSYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZY9</accession>
    <name>A0A8J4PZY9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">S-adenosyl-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_001336</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. RsmH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000031">
        <property type="protein sequence ID" value="KAF2077333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZY9"/>
    <dbReference type="OrthoDB" id="16290at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071424">
        <property type="term" value="F:rRNA (cytosine-N4-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.170">
        <property type="entry name" value="Putative methyltransferase TM0872, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01007">
        <property type="entry name" value="16SrRNA_methyltr_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002903">
        <property type="entry name" value="RsmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023397">
        <property type="entry name" value="SAM-dep_MeTrfase_MraW_recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00006">
        <property type="entry name" value="16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11265:SF0">
        <property type="entry name" value="12S RRNA N4-METHYLCYTIDINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11265">
        <property type="entry name" value="S-ADENOSYL-METHYLTRANSFERASE MRAW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01795">
        <property type="entry name" value="Methyltransf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81799">
        <property type="entry name" value="Putative methyltransferase TM0872, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2077333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="384" mass="43350" checksum="687F7848E653BCB9" modified="2022-05-25" version="1">MHKVFSNSIYKGSTLFTRTSGGSVCKRDYVTKKEKKIKNILSSAESGIGDDKKYVDVEYSSYEIHKPVLIKEVLRALNPTPGGIYIDTTFGLGGHTRAILDSCKDCYVIAIDRDSNVFDLTKDLRSKYKDRLITLQGNFSNLSTLLSENKLDKLEISGILYDFGVSSYQIDSSDRGFSYRKELEGPLDMRMNNQSNHSMTASNVLNTFSEKSLRDIFFVYGEEKHTKSIASEIVKRRVNEPLLMTSQLVNVVESCMPYPAASKTISRIFRALRMFVNDEIGEIKKGLHQGELLLKEGSPLVAISFHSIEDRILKRYLQRGVNPEKYGDESEALYLPSFDPHPLYGSGAPVYPSPEELKWNPRSESAILRTAIRNSNPPLPFKDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q045</accession>
    <name>A0A8J4Q045_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RWP-RK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_006374</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000292">
        <property type="protein sequence ID" value="KAF2072296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q045"/>
    <dbReference type="OrthoDB" id="20871at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003035">
        <property type="entry name" value="RWP-RK_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51519">
        <property type="entry name" value="RWP_RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RWP-RK" evidence="6">
        <location>
            <begin position="446"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="56"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="170"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="395"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="72"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="173"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="186"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="414"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="429"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="449"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2072296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="634" mass="71266" checksum="2C3EE761F347FBBB" modified="2022-05-25" version="1">MQKVQNPVSLPIGKPSSHIHSLLLPHQETQKDNSSFREQLFSNGWNLPYSKMANDSSIKKSNSSSFNDAFHHSSPAPSPSYDYSSSSSDEDEHSRVSRNISKFFNPLPTFKQICSESQLADNSRSSSLSSILQNSSSTSLNSSPSSTSSSPSYTVPMVNLQNHDQIRTIIDQPNSPSSSYPSSPSTAEYHTSSSTKNNQIRQWLDELNQLKKREYILLENLKNFNNINNNNSSSDESEDDHYPVIHNNIRQQQEPISPNVNIQPKQTLSIQQQPSDYIICNRYIHPSPVVVVDQETINRTTGYLIVNASLVSSPNPKQPKVNNKFELLQGVKSILVEKNGVVIFNKLKLNEISSKYSNQSFNICFTLSDISSDNQIHVLSQVYSTPFHILAKTNKRKSDEIENEEPNSPLGRQSSPVCSSPSSPSSPPQTVKSFDESTTIQSSQQQQSGKNSDNVPTSNGNDPNYIDITDLLILPQKEAAARLGISESMLCKRFKECTRRKWPYRYLRKIDKVIKILSFQNINEIPKEEKEKLERFIIEREECLRPVKIRITGCLEKDNDSPAVAPTVTTVDSQQQQSFKSSKIQNLINSGSVDETTPTQKEHANLFQAFIHGGESNGLENIVETLEMLKHTRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q046</accession>
    <name>A0A8J4Q046_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ubiquinol-cytochrome C reductase hinge domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_001287</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UQCRH/QCR6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000029">
        <property type="protein sequence ID" value="KAF2077438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q046"/>
    <dbReference type="OrthoDB" id="405848at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.20:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.20">
        <property type="entry name" value="Ubiquinol-cytochrome C reductase hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003422">
        <property type="entry name" value="Cyt_b-c1_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023184">
        <property type="entry name" value="Ubol_cytC_Rdtase_hinge_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036811">
        <property type="entry name" value="Ubol_cytC_Rdtase_hinge_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15336:SF0">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15336">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX 7.8 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02320">
        <property type="entry name" value="UCR_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81531">
        <property type="entry name" value="Non-heme 11 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ubiquinol-cytochrome C reductase hinge" evidence="10">
        <location>
            <begin position="12"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2077438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="69" mass="7569" checksum="E05EAC2EE1CDBF54" modified="2022-05-25" version="1">METSECSIKGPIQKECASGCGKTWAAYEACSERISKLVDDEKANCLGQFLEHVQCIDKCVAPKLFAQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0M5</accession>
    <name>A0A8J4Q0M5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">SecA family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_000940</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000020">
        <property type="protein sequence ID" value="KAF2077753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0M5"/>
    <dbReference type="OrthoDB" id="21121at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017038">
        <property type="term" value="P:protein import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000185">
        <property type="entry name" value="SecA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011115">
        <property type="entry name" value="SecA_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014018">
        <property type="entry name" value="SecA_motor_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044722">
        <property type="entry name" value="SecA_SF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30612:SF0">
        <property type="entry name" value="CHLOROPLAST PROTEIN-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30612">
        <property type="entry name" value="SECA INNER MEMBRANE COMPONENT OF SEC PROTEIN SECRETION SYSTEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21090">
        <property type="entry name" value="P-loop_SecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07517">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51196">
        <property type="entry name" value="SECA_MOTOR_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="7" id="KW-0811">Translocation</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SecA family profile" evidence="10">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="100"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2077753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="408" mass="46062" checksum="48AF2FA6DB33EBF3" modified="2022-05-25" version="1">MIGSRFSDGLHEFIEVKHGLLPERDTLTAAAISHPSFFAMYNTIFGLTGTCGEDNERKELQQIYHVDTFDVPSYLALKRIKMESLMFPDAQEYQQRIVQEIKEMLALSRPVLLLLPTINDSHVFSQLVQKEEGIHRVQLLNEMQMENEEVIITRAGEPKTLTIATNTAGRGTDIKLTSEALAAGGLHVIFGFYPTNIRVEMQGLGRSGRQGQPGSCRLIISKKDMESASLGLGLSCFEEFDEMRSLKCSLLSSSRLRRSHVDQQQYLLLDKFFALLAAVKSYCTKEKLVSLIQKSQSSSSPHITCACTPMQTTKIRKSLAFQNLYEASQKLDSSNTSSIETFATRLQSTINEYLLQEWADLFTSSFSNLSTFESTLENFDQWWISSSAQCFESNLEYHIITLIHLSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1G2</accession>
    <name>A0A8J4Q1G2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Dynamin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001667</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000041">
        <property type="protein sequence ID" value="KAF2077035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1G2"/>
    <dbReference type="OrthoDB" id="5061070at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF40">
        <property type="entry name" value="DYNAMIN-LIKE PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="2">
        <location>
            <begin position="176"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="507"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="507"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="834" mass="95888" checksum="43F85BD6E1C857EE" modified="2022-05-25" version="1">MSVFKKKDKSDDKKKKHDEGEKKNELNPVSANIQQQSIQATSATPLQATSPQQTSSTNGSIPNSTSNHSFTDETDRMKKEEDDKKKLQEAAAKQRKEEDDRIKREEQEKRRKEEEERKKSFAASEELNEEFEIQSLEQMGKLNSAVGNHDMFTFFPADVYQAYDKLQSFSRDLNTSVSHPEIVFVGPRGAGKSSLIEAFIGRALNIVGASTNLTSGTSKRPVYLQFVNNVECETPKVTMKKDNILKEFDHDLVIPFEQLNDNLAKRNSIYSEEPIYVSIEVKTALNLTIIDTPGLVANESAADQQKIDQIVNGILRPTHRLIIAVEACGDWSNMTMLSFVKKVDPELSRSTFVFTKFFNMIQDFNNTRGVNRFLSGTMSEIKSFFVTLPNHKIRARFSEPARYQEKINQAYKRDMQSLEQFQYDKRYERNIGVHPFRRYILNIVWKSYQDNIPRILKHLRSKRATAESALNDLQKQSGSLDSTMLRSIASNYTVTFLQINEKLISGTSEGNPSVNGQTLEEEKSQQGDGGEWVDLYNRPIKFDPEEWGIPYWNSKLYGGQQFERLMAEFKAVSDNTKISEVTMDDVATATGINKLNNIPNYAWAASDLASQKSQDALVPLIEQLCERAVYIMKRLADVTDKVVDSRKKNRIVGNNRLNSNNNGSIDMMDNIDQYPYFTHHVKDLYYKFIEQTAKNCKEKCLDEFYSTRTIYWELTEHPDQSLPHLRNDHQDTKAAVDHLATQLFDSIRGRITKNVLLKFYNFFLVPMQTDLWNEIQAKITCLSNDSLEQIFEVNATRDQLRDEEKNQQLILEKYSQNDEFFLKAASQFCHPLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q235</accession>
    <name>A0A8J4Q235_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tubulin-specific chaperone A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001979</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the TBCA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000051">
        <property type="protein sequence ID" value="KAF2076722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q235"/>
    <dbReference type="OrthoDB" id="296187at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048487">
        <property type="term" value="F:beta-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007023">
        <property type="term" value="P:post-chaperonin tubulin folding pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004226">
        <property type="entry name" value="TBCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036126">
        <property type="entry name" value="TBCA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21500">
        <property type="entry name" value="TUBULIN-SPECIFIC CHAPERONE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21500:SF0">
        <property type="entry name" value="TUBULIN-SPECIFIC CHAPERONE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02970">
        <property type="entry name" value="TBCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46988">
        <property type="entry name" value="Tubulin chaperone cofactor A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU364030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2076722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="127" mass="14914" checksum="32C56BE4FD2A3E65" modified="2022-05-25" version="1">MSSTTDIKKALKIKMDSLKRLENDYNHYRKEEIQQMEVIQRFKADKEKDIYDVKKQEEILDENKRMIIDSVTRIISYIFSFMEYLDDNGDSNDGSEIWNDAIGLIERLSDTFITNNPHNDGSIITSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2Q0</accession>
    <name>A0A8J4Q2Q0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_005616</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000229">
        <property type="protein sequence ID" value="KAF2073061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057709">
        <property type="entry name" value="DUF7949"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF29">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25820">
        <property type="entry name" value="DUF7949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035213332" description="EGF-like domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="1388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1341"/>
            <end position="1364"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="5">
        <location>
            <begin position="693"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="4">
        <location>
            <begin position="811"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7949" evidence="6">
        <location>
            <begin position="1059"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="3">
        <location>
            <begin position="1090"/>
            <end position="1314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2073061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1388" mass="155134" checksum="AB6BEAB051D784C4" modified="2022-05-25" version="1">MNKIIGLSLIVLFFYFNIYPVHSIPGYPFIDYSPEGTNDVFVNYLAGNVKQCNLRYNIVLSIFSNITELSCDSCASFQYSFVERNSTESLINIDIIYPYVPAQIAIPINVVGGGAAIGTFYLGFICEEPNFGIIEFVDKTLYYSYNLPSYPVFGFRVSGFNKKVAPPLTVTFTSTVSGTMTTKAISNNEYVVFVHYGYLSNDWSFFGKDFSIEISPAFSTGIVFQDSDFKSFYKIGSSESASYVVGPVNVIKDIIVNLYTVKPSRNDIFIASFIYSQNFIYPSTLVEANTSRTYYNSHAIDSASLSYNPFVVVNNSAILQNFPFTFLPPEVISSVSKTVQCLNSFVILDTKQNKYSPKFDIVTLIPGITSVPISSYQPYPFGFGSGNSSNYVLLKSYPVSPSFGWAFTLISGDDTLLYSPQVVTMDLDSEPPAIESVSVTKIPYSNLLLFTMTITDKISGFYSLEKFGDFTNVVSGSHNQVYQFIVKPYDYVRLYQGFKVCDYDSNCKKLNIFEPINLKGTIIPYSYLKLNQITGISFLYNNISTANSVENKLLIKSELIDPYLPVVMVLKDETKPDVSIDPMVYVGYWDDALQAYSISFPIVKYSAFGDFPYYLYLENQLLSYSDLRATFGPNATLKIIPGIVDIIGPMVFSLEAQANIPMTIEPSTGIIKIDSPTPGQRTLKSILPVYIFQTSFNLKITIVDAINGFKKGLLSVYTSVDLVQYNFTFDPTHLINGDKYSGVYKFDFQVYFDTCVSQDYSIYYLELMDELGHKTVYNSSFTSPNIMNPINQYYKVVASTLCTYIEDITPPALTNFTFWPSIIDPFNYGTKLNTSSRIIHFEFETYDLHKIKSEALPIIYLHDHSFQVISKRATLISNENTTAKYTCDFEIPFGFGHPYGIKASLYGIVDTRGNFNGYPVSQFPFPNAIKIQTKSTKPAIFSTSTLSPSGGFLTIYGANFFNTTIIDLEYSNLEPLDSFKPVYFNNRLIIVNNIPTLKPGYYYVTARTTSPVAAKEDSLSNSFRIVVGQDIPVYPDSSEASSSFENPPQECISNCNSQQQHGICTPKGCVCNYPWIGVDCASQVIIVDPTINTTHPETTLNDNVVNSIISIVGLNELDNSGAIINQYRFESWISRVNNNSHFSSSTNSFSYKTNITNKLDQSLTEIYAFIDYFNQSTPTNITFANQLITMNPHSLKYSINITSYSFSNSLNTLQLVLSASIENENGECSASSTGNNVESNSEFIKLQVNDNSLYGRFIKRGIVDGRVKAVTNQILKDSEFFQSTSTQSRSQAFIGINIPQYKRMVELDPDFSIIVDTRSAAESDNSICLGKKKSKLSTAQLAGIIVACVAVSAAIVTSTVYYLYKKKKENIENQRVAAKLKTLESPNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q2Z0</accession>
    <name>A0A8J4Q2Z0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_001493</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000036">
        <property type="protein sequence ID" value="KAF2077172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q2Z0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2077172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="141" mass="16560" checksum="46A8D1C6200BC0A2" modified="2022-05-25" version="1">MIIEYQGIRYNFNEELACGNKKQEREIKSVTDKLGDLLIAIPTVSKMKPQEAHETYLELQNLSIELRQLYKLIMAKSDLQTYQGIRWTSHTNLYSLLENILNKCLAHISLKRFVLIQKHKGEEALRKAFEEFEEFSDILNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q335</accession>
    <name>A0A8J4Q335_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000759</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000015">
        <property type="protein sequence ID" value="KAF2077959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q335"/>
    <dbReference type="OrthoDB" id="22922at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF169">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="452" mass="51417" checksum="124ECAA928719B6C" modified="2022-05-25" version="1">MTIDNSFYSIWRNRFLSIQIRKQVLENLDIKVDLQDLNANKQYLATLRNEKISLKLHIKSKEHFIEYLDHSEGYLVSNLEIDPKVEICRLPKLRQKQQPKLIGFDCRLIPNTVTKLKFWIEEAIMGYGKLPDSITELEIRSSWRTYSSKFFDHVLANLPQQLVSLTLTSNCDIRNQVVLPNSLCELKYSSTYDNLRKLVVPPSVKRGMVPPKLSSLFVDKFNEKLERDVLPDTLKYLRIRSFNQQLEPGVLPINLKTLTLDSFNKKLEPDILPPSLKALYLNSYTRPLDPYVLPQSLTKLGLNNFNHPLQASSLPASLTNLTMDSFCQSLESVGSLPNLHNVFIAQIDQYISNLLVNVKDVSLKFQKMTTTSTQPVSLSNTSIEKLVINSVSQTITIDCGFLPRTLTDLSMTGLAIASSSVIPEGCLFLKTDIVDLKPEFLPKSIITNKLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3B0</accession>
    <name>A0A8J4Q3B0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000701</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000014">
        <property type="protein sequence ID" value="KAF2077977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3B0"/>
    <dbReference type="OrthoDB" id="20276at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="326"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="353"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="385" mass="43866" checksum="DF4D1A3712FCB5EE" modified="2022-05-25" version="1">MNNNIKTILLKIIQFVLLIVSLVLYIRFSEIKYSSEFNEETSNQTFYYWFGGFILFITGWSIGTYKSFSKIILFFFIYKCAQEFVLHLPSCAYDHIRGTFDAVGQDTSNIAELFQQFAPQSLTAPFSNAVIATQLGLIVVFALLGNNVYLADKFWMIKTVIIDWVLAVILIIVTGISDLSNKQNNFFLVLSYIFGANILGYGVIKVCEFFWNYRSKCDDIKNAEIQQQQRQLQENVVFDTDNEFVNEESPVNTQQTDSINNSGEIQPVYDSLDYNSLSESQLDEILSAPSPSIPYTRKPQEKNKITFINNLVAPQNIHQKICAKEMVGCVILWIYSISNSMIQHFNIYSTSKILVVIGFSTTILAYLSTISSKRLGDKKPSSREC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3V7</accession>
    <name>A0A8J4Q3V7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000384</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000007">
        <property type="protein sequence ID" value="KAF2078292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3V7"/>
    <dbReference type="OrthoDB" id="4763727at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021243">
        <property type="entry name" value="DUF2804"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35868:SF1">
        <property type="entry name" value="DUF2804 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35868">
        <property type="entry name" value="DUF2804 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10974">
        <property type="entry name" value="DUF2804"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2078292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="380" mass="43349" checksum="4E1D403F2F987B03" modified="2022-05-25" version="1">MILKYSYSYINCKNCSSIKCICKDEIVGEVKKPVNMFDKKGNLMCQGWSRQPYNFQYNAPQSFFSSKKDNLYQFNEYYISTSQFSFNLVVNDFGKSNRFFIYFIKNSDKASPIYQQDEFCSKYLFKGIKFPSDINKPGTHARLNKKEFNIKFKVVKQGDQVLHLVRVKNEKLKLNADLTFDMSPKRESMVVVSPFRRGLYHFCYGNSNLVPVSGSLVVDGVDYLDPEECDQEDINAGLIWNRGYHSFSFIGVSAWGMYKNNKVALRIILGEQIPVAKSFINVNGVIHPLGNINICKEDLNESCFTFFSKNARFKSPVKFTPSKVQNVGFQNRAIHLLTGSIQGDIILSNGEVIKLDSFNASAMSNCLSINNSLTHLLDIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q444</accession>
    <name>A0A8J4Q444_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein mS38</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_000839</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the mitochondrion-specific ribosomal protein mS38 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000017">
        <property type="protein sequence ID" value="KAF2077877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q444"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013177">
        <property type="entry name" value="Ribosomal_mS38_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32035">
        <property type="entry name" value="AURORA KINASE A-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32035:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08213">
        <property type="entry name" value="COX24_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01155">
        <property type="entry name" value="DUF1713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal protein mS38 C-terminal" evidence="5">
        <location>
            <begin position="108"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00035647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2077877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="142" mass="15777" checksum="A3263047F5D63ABB" modified="2022-05-25" version="1">MISRSAQFLKSSISARTLTRNFTSMSISKNTNNTTLVGKPVSTTSTPSSSFTLSNQSSIPMLSFNNLMSTLSLEQVQKLESSINLSNFASPSPIATPIETEKETVVAELSSVILKRRKKMSKHKQRKLRKRMRALKKKLGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4E2</accession>
    <name>A0A8J4Q4E2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000080</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000002">
        <property type="protein sequence ID" value="KAF2078580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4E2"/>
    <dbReference type="OrthoDB" id="1716625at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053086">
        <property type="entry name" value="RhoGEF_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45834:SF7">
        <property type="entry name" value="DH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45834">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="2">
        <location>
            <begin position="725"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="433"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="643"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1032"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1111"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="260"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="500"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="510"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="523"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="540"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="596"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="647"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="659"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1039"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1111"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1174"/>
            <end position="1184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1191" mass="131709" checksum="C994569ABFB173D9" modified="2022-05-25" version="1">MSSKTIKCGSCSVLNSDQSRFCTQCGTKMPPPALTSAGSSKFLAPVLKPAPTGTSTQSGTLKPVTSTTLTTTASRPSSTIVKSTTSTSTASSGAKPPTTTTSVSSFLKPSTTTTTTAAAGLKKPDTPSTTGTLNRPKSHTVSYKPTTNTLHKTTSSGPLPSTTTTAPTSEPSWKPTGSFVKMAAKSRETLDAQATRDSMVLQGAPITTTAVSSSKPPVVTNTAHKKTISTPGVSKPPTTTTTTTTNTPKSPPPPTPNRPPSMSVSHSPSSRPPSTFVSSPSSPSRPSSTVVSKSSTQHSYEEKKEEMEKQIQQEKERLHQKEKEIEKQRQAQLEKDKEDQRLKLQQEREKQLEKERLEKEKQQEKERLEKEKHDKKEQSEREKIEKEKQKQAEKEKQKQMEKEKELERERQKQADKEKIEKEKQRLLEEKQKIAEDKQRLQEERDKQKQLLEKEKLQEKERQKQLLREKNMSNSPKDESFLSSFISTRKDSISLSRSSSPATVQPSHPLNGSFDNGGSNNDNSMDHSNDSDDENRPVSPISFSDIMNGSKHSGDESSGTSTTMKRKKDKEKYSTRLLSFIRGNEKDREGEDDFVISSPSSPSPLINSSADSLDTANQSGEEDGPQLRKWAIKREELRKSEKDLFAYSPSSPNNSSSNSVTNGGTMSALSLNGGNGSNPTPPMSPTIGFVPSVSSPGEGRVMLGGSRISSPNPPPDYPTLPASTEQRDRVIQEIICTEADYIRDLEIMVWLKKELSAQDDIKVSGIEEINSLFSNVEQLLMVNRELYRKFCNTDQSVKTDDFADYIANGFFSMADFLKSYFVYCSNQQKALSTFSNMKGKNCTYLAYLLTRRECRSLPFDSFLIKPVQRVCKYPLLLRELIKSTPNTTETYKSLTAAQTKIEGIVLTVNEKKREFDSQMKMYDIQTRLVESGDNTKILSPSRKFVKEGVMESWGHIAPTQGTQYNKKPKEGQFYLFNDLFIFAQVKSSGANLISSVMPIKVKANLLLGQSLIREVPNDPLSFEIVYIPKDNNTADHHHSSGANSTNSNDPDQESNQHEHVILNPQNSTIWMFSMSTPEEKNAIFSEIGALIESILKNEYRKFIEDRQKEKAAAASVSPPSSGPSLNVSSSALSSTSSSSEIEFDEWRKLVKNKPLPAPPVPNAGSPSVTRLSKALPPPPVPPPPSTQTISTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q8V8</accession>
    <name>A0A8J4Q8V8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">S60 ribosomal protein L31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001685</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000042">
        <property type="protein sequence ID" value="KAF2076976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q8V8"/>
    <dbReference type="OrthoDB" id="9739313at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00463">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.440.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000054">
        <property type="entry name" value="Ribosomal_eL31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020052">
        <property type="entry name" value="Ribosomal_eL31_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023621">
        <property type="entry name" value="Ribosomal_eL31_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10956">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10956:SF0">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01198">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01380">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54575">
        <property type="entry name" value="Ribosomal protein L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01144">
        <property type="entry name" value="RIBOSOMAL_L31E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2076976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="113" mass="13134" checksum="957222C60CD94D29" modified="2022-05-25" version="1">MSTKTTKEPISREYTINLHKRLYGTTFKDRAPKAVKQIKLFAQKTMGTRDVRIDNKLNTFLWGQGIKNVPHRVRVVLSRKRNEDENAEEKLYTLASLVVVKSFKKLQTKVVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q9D7</accession>
    <name>A0A8J4Q9D7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_001450</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000034">
        <property type="protein sequence ID" value="KAF2077261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q9D7"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004508">
        <property type="term" value="F:steroid 17-alpha-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042446">
        <property type="term" value="P:hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042448">
        <property type="term" value="P:progesterone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20617">
        <property type="entry name" value="CYP1_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000068">
        <property type="entry name" value="Probable cytochrome P450 508A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24289:SF21">
        <property type="entry name" value="CYTOCHROME P450 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24289">
        <property type="entry name" value="STEROID 17-ALPHA-HYDROXYLASE/17,20 LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12 13" id="KW-0349">Heme</keyword>
    <keyword evidence="9 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2077261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="485" mass="55362" checksum="0A04A8E5C206F843" modified="2022-05-25" version="1">MYFFWALLYSFFAYLVFKYIQKFKNYGPNALKGPFPLPLVGNLHQIGSTPHKSLTKLHDTYGPIYRVWMGDRYSVIVNDIQLMREMIVTNNDTFLNRPKSPSLKYYSGNYISDVSSSSDEVWKRHRDIAAAALTKTKMKHIYTLLDQQVDDLLTSMSIVEKSGQAFEPRRYSQRFTMNTMLKYIFNKHISYDEDVNSGLMKEIIEPSDAIFQELGTGALGDFIDIFKPFTAMYLNLFDKNAPALINFAIREYKEHLETYTEEHKATPRDFMDVLITEFSDDPDKVDKIAYMSIDVLLAATDTTATSIEWAFLYLANYPEMQEKAAQELVSVAGKDRNLSLADRQSTPFTNALIKEIARHKVIGPFGLIRHCSADIEIGGHFIPKDSQVLFNYMSIGANPKYWENPSEFNPSRFLGPNPQDFPMFSLGPRSCVGKSLALDELFLGIGNVIKKFKVSSTDGKPISDADIFGLTIRPTKFYVTLKERN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4QA75</accession>
    <name>A0A8J4QA75_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">4Fe-4S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_000823</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I 23 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000017">
        <property type="protein sequence ID" value="KAF2077861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4QA75"/>
    <dbReference type="OrthoDB" id="204405at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3270:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit I 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01351">
        <property type="entry name" value="NDH1_NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010226">
        <property type="entry name" value="NADH_quinone_OxRdtase_chainI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01971">
        <property type="entry name" value="NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004538">
        <property type="entry name" value="PRK05888.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004539">
        <property type="entry name" value="PRK05888.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849:SF20">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 8, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 8, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="107"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="146"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2077861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="215" mass="24491" checksum="A5B3755DFA982D61" modified="2022-05-25" version="1">MSMITSKFVKGASSQLTTPQTFGAVCLGTRNFHSSKPNLHARWDDQVDQRPTFKKIIDDTANTFFLTEILNGLYITMGFFFAKKCTINYPFEKGPLSPRFRGEHALRRYPTGEERCIACKLCEAICPAQAITIEAEPRADGSRRTTRYDIDMTKCIYCGYCQEACPVDAIVEGPNFEFTTETRLELLYNKEKLLANGDRWETEIASNIAAEYLYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4QBL2</accession>
    <name>A0A8J4QBL2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">THO complex subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_000081</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000002">
        <property type="protein sequence ID" value="KAF2078581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4QBL2"/>
    <dbReference type="OrthoDB" id="205166at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008501">
        <property type="entry name" value="THOC7/Mft1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05615">
        <property type="entry name" value="THOC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="194"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="81"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="194"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2078581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="217" mass="25466" checksum="8FA8219D0BF1229A" modified="2022-05-25" version="1">MEDEEDVILKNRLIYKDAIIKRSFKKYLQFINILTDTSDTTNSNSSEKIQSAYISLIKDLSNFELGIQKATTISETSKEELQYYETLFKQKELEIENEKQEILKLKERLAYEKTQRQYKEQYLALYKLINEKPSIDQTEKEIEIAQKELNEISDQTLKTNNKLELRSKQFQLLLHTLGELEKNLDDDNQVIVQQQQSSNMATSTTTTTDNNVDKMES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPF0</accession>
    <name>A0A8J4UPF0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009529</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000732">
        <property type="protein sequence ID" value="KAF2069151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UPF0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="605" mass="70756" checksum="FE21B620562E7B08" modified="2022-05-25" version="1">MNNSIDQLYRSIFNSLVLSKRIYSLVHEVQYNHNSLRYNDIVDVKWMIKYNHTKLLRDKVQRDCLLLNHGKSIHRLVNKDIELFKTIIMKYKCCTWDQEMFTKIIGTNNYEAVKWVCEMGFGVELDDEHMMFNEKSDPKILDYLLGKSLYKVTFDSVVKIYQSTYSKFTLPILDVFKQYTPTPIVKWQADMIVNVLLKVPIPNLFMTLSPLFQENIKLSASLSDIDSSSIDIYPILCYLKEKDQWEQDSKEYEELVSIVDSVAILVKVSSFVDAALLLDHRTSIDSIKQLVLEFFPEPFNNENNERMKDSDSIRAKLFNLSPEVIELLLDHGLEFQAGWFKEILEFDLKHLFISLWNIFKKRDQVSVTPSHCYFRPNPQHMDQSFIIHNLFLADIQVIEFLLNEGVHPDSLRTYSYNHHMALAQEAKTESHFKSIQEFIKLFILDTFLMLSICIQSGNIQLFHWIRPLTGGHLISRLIEGAVLFKNIDILNILTSNSSNSVSSEGIWKDTKHMKLKFLKEYFASLDEQQKLESLPLIKGIKSLIDENRYEIVKFLLANVANLSIKHYTPNLVNSNLAMVDLFYQNRDTLKLPSPFSWDELFKNFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UQF0</accession>
    <name>A0A8J4UQF0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein-serine/threonine phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_008096</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000471">
        <property type="protein sequence ID" value="KAF2070591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UQF0"/>
    <dbReference type="OrthoDB" id="10252009at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46381:SF2">
        <property type="entry name" value="MAP KINASE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46381">
        <property type="entry name" value="MKPA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01908">
        <property type="entry name" value="ADSPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="4">
        <location>
            <begin position="245"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="5">
        <location>
            <begin position="309"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="99"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="186"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="99"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="186"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2070591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="438" mass="47863" checksum="1696EFCB114A9290" modified="2022-05-25" version="1">MTSKNTVTTSTIAAATSSPASSTTTDNQQQKPQLQQQQPAAATRQSSFLLKRKGLTINIDRSNEHPNIESDENIYLIKEGEDINDCSGFRKPQSLTQSSVIPVNNNNNNNNQNNNNSNEPLSPSLSPLSTSSSAFTDSESSAPNTPVSSNYFNCTPKSLSQSAPSIKLKINLNNLNNNQQLRQLQQANSGGGNNNNKSNTTTTTTTTSSKELGNSSSTSSSDDEPPESSRWLGCDNDKFKIFDPICSKVTDYLYMGSETVASDREKLASFGITHILNVSHQCANFFEHDTSLVYKKFPLLDNPTEDISLIFNQAIEFIESACAVNGRVFVHCQMGVSRSASICMLWIMKATRCSFEEASDLIKLIRPISRPNAGFQLCLLNWAIKEGIQAPNSHRCNNIDINTISARFQNLSLAPNILANLSSLATSEKSSFTIDDSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UR00</accession>
    <name>A0A8J4UR00_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">L-fucose kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_007260</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the GHMP kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000377">
        <property type="protein sequence ID" value="KAF2071416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UR00"/>
    <dbReference type="OrthoDB" id="271303at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050201">
        <property type="term" value="F:fucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042352">
        <property type="term" value="P:GDP-L-fucose salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012887">
        <property type="entry name" value="GDP_fucose_pyrophosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052203">
        <property type="entry name" value="GHMP_Kinase-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32463">
        <property type="entry name" value="L-FUCOSE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32463:SF0">
        <property type="entry name" value="L-FUCOSE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07959">
        <property type="entry name" value="Fucose_pyrophosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GDP-fucose pyrophosphorylase" evidence="8">
        <location>
            <begin position="136"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="7">
        <location>
            <begin position="920"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="9">
        <location>
            <begin position="1090"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="58"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2071416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1203" mass="133341" checksum="64BE49E964D92670" modified="2022-05-25" version="1">MAFWDAIAVTAPKNEHAQEFLRELQTRQNEGLISKSTFLISIPDPLQDEDSFRNIGLVNQQGVRKDSGDRENSDIDEPQSPSNNVQQPPPPIVGIEKDKLAQGFAKLGSGTSTINSLFVICEKLSALSGKSYLDVDVLKDKRILLLLIGGIYQHAPLVNMCTKSFSMLPMIGVNLNYDDPSRAVDTYVGDPPVYSIDILLSNLNVIIPHLQPGFMVASTESMILFDQNDANLKDDQVWRKPGVSVITMDVDSDCYKNHGVCKIDKETNQIIEMSYKKPAEYLEKSGFIDENGKASLYTAILFFCQKSAEKLLYLYNTPPISSCTYLGIDSGTQMLKFGVYPDILCTMTKNETLDSYLNLPTFHNCNRNLVDKGRRVLWNTFRGIPINSIKIKGEFIYLKNSEDFLNLVYYNKNVKVSKRVHSYIDCKKPIEGIIMNSILTGNGSVEKTSIVYSSVLTGNWSVGSNSIVLGVKGVSFGFEILDNMIVNEIRLKSDKIKLMAMDSQSNTEPKALVVLGIHDDLNCLFDDPNATIANRSWSEFFLHSGISPDELWPKCVSKVLRTARLFPIITCDDEKLVEASLWIQSHGSPSLSVVGRWRSSKRVSIADIIGESHLLDYSQLESVAEKKKSLKLMSKQIIEGNIEAEFRWRRALSFKVDSLQVEKTLTQGEDVSILPFLHKWADSGIPIREVLDTLDRIALSSPIKYTGRLLSCISDALAIYVNHKGGLRSGPAKNSAWDKAFSYFRSHDERKGLLALAKEREKWLDSYENMIRAARHYEGAGQLVIKNIVDTCKTVLIPLCADNGDQVILPNKDWIEVKIPARIDLAGGWTDTPPICYEHGGIVINASVKVNGEFPVKVTARKVSDPKITIYFDSVESDPVICTTLLDLSDYNQPLAPGALVKCCFLQLGFIDINSTKASLEAQLKKLGGGFELISSSSLPTGSGLGTSSILAAALLFAIAHIYGYKYTDQHLIHAVLKVEQMLTAGGGWNDPAGAVEGGFKEAKCSKFSDQQQSAASNGSGSNGSITVTYDKIPVSRESTDMINRHLVLIYTGRTRLARDLLQDVIRRWYSRTTEILSVTENLLANTVDMKKALLDGDLASIGKYLSVYWKDKKCMAIGAEPTRITEIFSLINDHVYGSSLTGAGGGGFLIAVCKEDKDIFKSKMKTAIEQVDGLEDIRFYDCEVCLDGLNFKIIKSTEEQVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVJ5</accession>
    <name>A0A8J4UVJ5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ComC supersandwich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009951</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000863">
        <property type="protein sequence ID" value="KAF2068730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVJ5"/>
    <dbReference type="OrthoDB" id="18358at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF29">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="2">
        <location>
            <begin position="2"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="198" mass="22201" checksum="537235AEE5CB3621" modified="2022-05-25" version="1">MNQYSIKYSINITSYTFTNSLNQLQLIMKVSLESQQDQGCSALESGNTTVTNSEYVKLQVDDHSLYGRFIKRGIIDGRISTITNQLLPNYNNNGESNQFNNIQSYIGIGIRSYRRLVQLDPDFSVLVDQRPASNSQNESTCSSSKTKKKLSGAQIAGIVIGSVAFIAIIVVSVVYHIYKKKKAIQFNKQVENKLKNMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UW44</accession>
    <name>A0A8J4UW44_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_001497</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000036">
        <property type="protein sequence ID" value="KAF2077176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UW44"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="258"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="338"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="48"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="187"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="261"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="286"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="340"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="403"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2077176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="428" mass="49314" checksum="C9F4724AD10C43A8" modified="2022-05-25" version="1">MEDNNNNIDTNNYTDMDIDKLKMDFDLDYLKNYAILLEKSVSSAINDFQEKEKKLIPQNDEINRLEQEYKKLLDDHSDMHRKMMLLESQVDNLEFLINVLKKSGDDLNKYELQLKEQIAKLQYNKIIVGEFHKQKIQDFDSWVELHSDPALNNKNKCEEIDLKNIQLTKLKDTVNQKNYLYNECIALEKAIKEFDELEKKNQSIIDKLITEIEQIEKVDDQELKNLETRIREKKQNLLKSKPLSLSSSYNNIQTKSLQFKNNNNGQNNNNNSINNNSNNNNLNLNTPISQPSKYMPSLNNTGNKSTLTISNNNMKSLNQITPLKLKTAQLISKPITVNKSQNSQNSQNSQNNNQTPVATPTRPTTTTTTTTTTNNTKTNNSNNLPINSSSNNNINATNNNNSEEMSIRNNSQSKKFGPIQRNFMTRPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UXB1</accession>
    <name>A0A8J4UXB1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007996</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000458">
        <property type="protein sequence ID" value="KAF2070695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UXB1"/>
    <dbReference type="OrthoDB" id="27237at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010770">
        <property type="entry name" value="Ecd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13060:SF0">
        <property type="entry name" value="PROTEIN ECDYSONELESS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13060">
        <property type="entry name" value="SGT1 PROTEIN HSGT1 SUPPRESSOR OF GCR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07093">
        <property type="entry name" value="SGT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="506"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="575"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="686"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="512"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="578"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="599"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="686"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="755" mass="87569" checksum="125C88BD2B36DB1A" modified="2022-05-25" version="1">MKNDPNDYLNDNNDENDDYEQLDFQDNDDLKYKDIQYVKYRLYDDNLYNNSNSLLTKDQQKNHYQQLLNDINQFIIPYYDTYIWQKDPFKLKLFDKESNKKLPLYHIYGRTEFDELIDDEWFIVYLLIEISKRFTNLSISIKDNDGQFLLIEAAKTLPKWIKPTSIKNRVFIKNAMLHIIPLPTNPSLIDVIPYRMNVFNALEILSNPSKYPTIKTNVSSDTIKVILDRINIFNNNGYYNEQNHIRNVYLPAEISYLLIRYPQLLSEIVSTFYNRDVDDMKSVTKMERFGKIASDSEYIESKIRFTRCLYAQIKLQEFHLPPNRGNGLSMPSIKDPLYESKLLGTKLLAALEMLYANAQRKTLFERYLDEPPDLDTYKFHIDSYWIGYKEHLVKSGYFEGEREGSARFKHKLQIAKQQYIDDKLYLKNIQQPDQQEGGPAVDKVGSKFKIASLIDETINWTKSSDGKKQMMDTIASFKNSNPTSSDEWLQDSPEKLEDLITLFEKQQQQEQQQEKGNKGNSKSKDTVGLFSKSFKSMLSQVSSIDGIDFKDDKGTKDGVSFDSKKFVNILKSLVGGDIDNDNDQDDSEEEDPMYQSINDFDDDDDDDEENSDDDDDQDDDQDDSEEDTDSEEENQDSDSPEEYIKFIKRGSMKSIMKQMDQELNFKSVGDSFEKVLDDKDVETILQQQDNNSTGQPSTTTTTTTQPKQEGSAVKKVDLNLNLVKNILESLSEQHGLAGPASNLLRELSDNQRIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UY35</accession>
    <name>A0A8J4UY35_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">adenine phosphoribosyltransferase</fullName>
            <ecNumber evidence="7">2.4.2.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_007308</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:16609"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.4.2.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.7" evidence="7"/>
    <dbReference type="EMBL" id="AJWJ01000382">
        <property type="protein sequence ID" value="KAF2071368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UY35"/>
    <dbReference type="OrthoDB" id="363185at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003999">
        <property type="term" value="F:adenine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000004">
        <property type="entry name" value="Adenine phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050120">
        <property type="entry name" value="Adenine_PRTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002636">
        <property type="entry name" value="PRK02304.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11776">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11776:SF7">
        <property type="entry name" value="PHOSPHORIBOSYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="12">
        <location>
            <begin position="58"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2071368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="198" mass="22130" checksum="DA8477217A0145DB" modified="2022-05-25" version="1">MQSTYSELLASAKQSIIKQCNVYQDYPVKGVGFLDISPIFMSTVDFDNVLTYWQHRFTNVDLVVGLEARGFVIGAAFAQKIGAPFMMIRKKGKLPGQCISEAYVKEYGADEFEAQVNALSKTSFPSDRKVNVLIMDDILATGGTLCASIELVRKLLSHNNVKEYKISTSLISSIKVLRGKEKIYEKYNDVSIDVIHEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZN9</accession>
    <name>A0A8J4UZN9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA (guanine-N(7)-)-methyltransferase</fullName>
            <ecNumber evidence="9">2.1.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">tRNA (guanine(46)-N(7))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">tRNA(m7G46)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_010571</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42708"/>
            <dbReference type="Rhea" id="RHEA-COMP:10188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10189"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74480"/>
            <dbReference type="EC" id="2.1.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">tRNA modification; N(7)-methylguanine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.33" evidence="9"/>
    <dbReference type="EMBL" id="AJWJ01001206">
        <property type="protein sequence ID" value="KAF2068102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZN9"/>
    <dbReference type="OrthoDB" id="47276at2759"/>
    <dbReference type="UniPathway" id="UPA00989"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043527">
        <property type="term" value="C:tRNA methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008176">
        <property type="term" value="F:tRNA (guanine(46)-N7)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000309">
        <property type="entry name" value="tRNA (guanine-N(7)-)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03055">
        <property type="entry name" value="tRNA_methyltr_TrmB_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025763">
        <property type="entry name" value="Trm8_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003358">
        <property type="entry name" value="tRNA_(Gua-N-7)_MeTrfase_Trmb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00091">
        <property type="entry name" value="tRNA (guanosine(46)-N7)-methyltransferase TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417">
        <property type="entry name" value="3-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417:SF16">
        <property type="entry name" value="TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02390">
        <property type="entry name" value="Methyltransf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51625">
        <property type="entry name" value="SAM_MT_TRMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 9" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2 9" id="KW-0820">tRNA-binding</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="90"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="123"/>
            <end position="124"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="221"/>
            <end position="223"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2068102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="270" mass="31298" checksum="320C4AE1CC4B8B09" modified="2022-05-25" version="1">MSNQVVKKKKAIKPYHRIKAHANPASDYHFYYPVSPDQHDWSKNYPDIIKADDEESKKKRVEIADVGCGYGGLTISLGTLFPETLCVGMELRDKVVQYVDERIVKLREKTPGSYQNISVIRTNGMKYLPNYFHKGQLQKIFFLFPDPHFKKANHKRRIISPTLLSEYAYILAPGALAYFISDVEELYLWMYEHFKNHPLFQQVERHVAESDPCVPLIINSSEEARKVNRIDGKKWYAVFRRIEDPATKDKSFVNYTLTSPKESTTTDASI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZQ4</accession>
    <name>A0A8J4UZQ4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005287</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000206">
        <property type="protein sequence ID" value="KAF2073408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZQ4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="94" mass="10650" checksum="5116700A715F173D" modified="2022-05-25" version="1">MEEEYRDIPKTNKTQGVRQWKNEIPGASERLANAEKIIGQNDLHGGPASRLKTVKNADKDPSEIKERIDKIQEITGKDVDDQVKSGYWEGKTIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZR7</accession>
    <name>A0A8J4UZR7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010452</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001123">
        <property type="protein sequence ID" value="KAF2068222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZR7"/>
    <dbReference type="OrthoDB" id="2272012at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051092">
        <property type="entry name" value="FYVE_RhoGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673">
        <property type="entry name" value="FACIOGENITAL DYSPLASIA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673:SF262">
        <property type="entry name" value="RHOGEF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="1">
        <location>
            <begin position="33"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="111" mass="13187" checksum="71FCC5AEE5BA9BC2" modified="2022-05-25" version="1" fragment="single">MRDLETSAIVIQKWVRGWLVRRDHVDFFKKIKYRKFIIREIINFEKTYITKISLLINELKKPLLEQSIVNIDIVDQLFSNIDEILDLHVSIHNTLTATQPPIQIGLIYLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZS3</accession>
    <name>A0A8J4UZS3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005216</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000202">
        <property type="protein sequence ID" value="KAF2073468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZS3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="464" mass="53375" checksum="5B733D33E2802653" modified="2022-05-25" version="1">MCFNTTISIDSIDSIDVCDPCYSSLSTQDKIDYHIFIKLVITTSKDTQRFLDHPNRHIVNEIDFNEPFFLFAPEEGTRDVLLVNFNTLPNTIHKLSCEVNSDQRIIGTVPSSLRELHLRHIWDEDREYLEFNSMFIDHLLSNIPPTLEVLTLPGNFSSRMTECVLPTTLYDFRYRTIPPNLSKLRVPTNKVYSGCYLSTNRYLKKRWLESRTWITGIILGNYDYLEASKPIPSQVRRLELIKIYGASEDDPPPPLILVPGWLHNNITYLSIDDLNSELEFGILPEYLETLKIEIYNVPLKPHLLPNTLKYLCLSSYDQPFFVKDSLPASLTRLELPSFKGSFEQLHPLPSLTRLYINEMNQSITTIIPTVKIIYIKLRSFENNQVTLQPTVLHHLEFQTYNKSELKPGFLPSSIRSLVLKNCIVESTGIIVDGCASIRSDIKIDPEFLPHSVKFITLSPISIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZX6</accession>
    <name>A0A8J4UZX6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005033</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000191">
        <property type="protein sequence ID" value="KAF2073648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZX6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="262"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="224"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="306" mass="36418" checksum="E423D6ABA2F0ED6F" modified="2022-05-25" version="1">MERKHIKKYYKFIKSYVNKGPDAWEKSGAHYWKKGWGARQVPPVYFYDDLDVDDYFSHVLDFIEEFQLREREELKEEKPLFVHDVYIENFTILNRVQSLVKNILNNERKWTKEQEFYEDDPLEYCLFSNQFSINMQSYLIEAQQRSPNRIKTKLTLAQTFPDVVMLPDEDDNEYDDEDYEDDDIISISNSISGSIEQDNQSSEIEFITKTLEDIQLQIQKIHTNKNNNNNKNNKNNKKKKTKDIETMSNTLEDIKLKIEILNTKNNQKELKETESISSENDNSDSESESDSNPNRYQKKNIPITRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V012</accession>
    <name>A0A8J4V012_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2 5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_003696</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2 5"/>
    <dbReference type="EMBL" id="AJWJ01000119">
        <property type="protein sequence ID" value="KAF2075000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V012"/>
    <dbReference type="OrthoDB" id="1902587at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000006">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044609">
        <property type="entry name" value="FKBP2/11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45779">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45779:SF7">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0697">Rotamase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035274253" description="peptidylprolyl isomerase" evidence="6">
        <location>
            <begin position="20"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="7">
        <location>
            <begin position="44"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2075000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="133" mass="14466" checksum="6BFB8FC390BC0A26" modified="2022-05-25" version="1">MKFLTILLFVSLILGLCLAAKPSDKLQIGVKFRPETCTQKTVPGDHLKIHYTGTLLNGEKFDSSVDRGTPFEFTLGEGRVIKGWDQGLLGMCVGEKRKLVIPPSMGYGAHGSPPKIPGNSHLVFETELIGITN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V0P4</accession>
    <name>A0A8J4V0P4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mediator of RNA polymerase II transcription subunit 31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_004033</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the Mediator complex subunit 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000136">
        <property type="protein sequence ID" value="KAF2074638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V0P4"/>
    <dbReference type="OrthoDB" id="10257739at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1340:FF:000001">
        <property type="entry name" value="Mediator of RNA polymerase II transcription subunit 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1340">
        <property type="entry name" value="Mediator of RNA polymerase II, submodule Med31 (Soh1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038089">
        <property type="entry name" value="Med31_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008831">
        <property type="entry name" value="Mediator_Med31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13186">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05669">
        <property type="entry name" value="Med31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0010">Activator</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="285"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="68"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="139"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="287"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="305"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2074638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="325" mass="39199" checksum="11024E154B7C19B4" modified="2022-05-25" version="1">MSRRRAAKSAPKVKEDDGHAMAVEQPKVTTNVDIEEIKEKTPPPQTTVTSPDNKDDKEVVMVDVDDDDTKKEDKQEQEQERKEQEEEKILVDKEETQQDKEVVMDVGDDDKKEEKQEEERKEQDTIVSDKEEEEEDREKEEQEKDNQDDLKEKEEQEDKQDKDEKEEQPPTPMTEEEEANHIRFIMELEFIQCLSNPRYLNYLAQNRYFQDKAFVNYLKYLLYWKKPEYAKFIVYPQSLYFLDLLQEERFRQELNHVVSTDFIHEQQFYHWQYYRNNRMSIKEEQLQQEQLQQQQQQQQQQQKQPTPQDSTNTAFVNPTITTPTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V213</accession>
    <name>A0A8J4V213_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_007672</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000422">
        <property type="protein sequence ID" value="KAF2071012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V213"/>
    <dbReference type="OrthoDB" id="19473at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003333">
        <property type="term" value="P:amino acid transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018227">
        <property type="entry name" value="Amino_acid_transport_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16189:SF6">
        <property type="entry name" value="AMINO ACID TRANSPORTER TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16189">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 104-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03222">
        <property type="entry name" value="Trp_Tyr_perm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="342"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="405"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="470"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="610"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="639"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="678"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="188"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="518"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2071012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="699" mass="78554" checksum="6F03F92D3CE8A7CD" modified="2022-05-25" version="1">MINSNSNTTNNNSNNNNNNHNNNNNEDDEDNLLIEDYEKSSPSLKDSSISFFKSKIFSQSQKENNNDSGQASGEKTPLLTLAYLYFIVIASVLGTGILALPVKLAHSGFTPFVASYTLCYTMQLLSIFFLIEVLQRIYSIIRSNSNFSPTLDDNQDEDSLLKDKNSGKHLIQNSNQYEISMEPLNSNFGNSTSLTDENNNNNTINSESSSPRLDDTDHDEVSLNSSTTYEKATSIALHEDHDGVHGDNNDHNTSHDATPNLHIIGLKFLGPISYTIFICSVLLHFCATLISYGLAGSEAYAQLFNIDHQYIILPVVVGFASIVIFGSNVLQHIITFFTFGKGTLLATIVFITGIISHQVETDIDNNWSFIGKSFLISTVALGGAGSVLPSVFNKIIFSKKEMMKCYIVVGSALTTIYVLNITWCWFLLGIIPQLPNPNDPAMPSLEAAAESGSIATIPLMKMVKLYYPQFLWITRLIDIFIVVSITISFITVGSGFKHTLDGFSQTWRTKSLQNSFHQESLRNSSIKSSDNDDDDDYNQQSSKINENYFGLFGKVKRIIHNITYWFNFQYQRFQQLIHGCINNVNQFIDSKEGQSTMNNGTIVRFPIREFIFYGLFFGFILLIALLNPSGFLQVMEGGSSLGLNLQTGTFMVLIISFSRKNYENYSIPLPVHDIIFKLRYVVFCYFTFAVFYDFYVILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2B1</accession>
    <name>A0A8J4V2B1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">dolichyl-diphosphooligosaccharide--protein glycotransferase</fullName>
            <ecNumber evidence="6">2.4.99.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">CYY_007334</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a di-trans,poly-cis-dolichyl diphosphooligosaccharide + L-asparaginyl-[protein] = N(4)-(oligosaccharide-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl)-L-asparaginyl-[protein] + a di-trans,poly-cis-dolichyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22980"/>
            <dbReference type="Rhea" id="RHEA-COMP:12804"/>
            <dbReference type="Rhea" id="RHEA-COMP:12805"/>
            <dbReference type="Rhea" id="RHEA-COMP:19506"/>
            <dbReference type="Rhea" id="RHEA-COMP:19509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:50347"/>
            <dbReference type="ChEBI" id="CHEBI:57497"/>
            <dbReference type="ChEBI" id="CHEBI:57570"/>
            <dbReference type="ChEBI" id="CHEBI:132529"/>
            <dbReference type="EC" id="2.4.99.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endomembrane system</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the STT3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.18" evidence="6"/>
    <dbReference type="EMBL" id="AJWJ01000384">
        <property type="protein sequence ID" value="KAF2071357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2B1"/>
    <dbReference type="OrthoDB" id="10261066at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004579">
        <property type="term" value="F:dolichyl-diphosphooligosaccharide-protein glycotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003674">
        <property type="entry name" value="Oligo_trans_STT3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048999">
        <property type="entry name" value="STT3-PglB_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048307">
        <property type="entry name" value="STT3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872:SF1">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT STT3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02516">
        <property type="entry name" value="STT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21436">
        <property type="entry name" value="STT3-PglB_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="159"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="188"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="286"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="316"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="404"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="427"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="466"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="Oligosaccharyl transferase STT3 N-terminal" evidence="17">
        <location>
            <begin position="35"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="STT3/PglB/AglB core" evidence="18">
        <location>
            <begin position="529"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF21436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF2071357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="705" mass="79057" checksum="4C82D320BA5D147B" modified="2022-05-25" version="1">MSGKTASGASNSAGQSANLAIASMKSIPKENQEFLLKVLILSIIYVLAFSTRLFSVLRYESVIHEFDPYFNFRSTKYLVKEGFYNFLNWFDENSWYPLGRIVGGTIYPGLMATAALVHWVLNQINLTVDIRNVCVLLSPWFASNTAMVTYKFAKEIKDTQTGLVAAAMIAIVPGYISRSVAGSFDNEGIAIFALIFTYYCWIKSVNTGSLLWSCVCSLAYFYMASAWGGYVFIINLIPLHALALLVTGRYSHRLYIAYSTLFVIGTILSMQISFIGFQPVQSSEHLAAIGIFGLLQLFAGLNWVKSHLSAEGFKKLQRLTIISVLSVAGAVLFVGTVTGYISPFTGRFYSLLDPTYARDHIPIIASVSEHQPTTWASYFFDLHILVFLFPAGLYFCFSKLTDSNIFLILYGVTSIYFSGVMVRLMLVLAPVACVLAAIAVSSTLTTYMKKLKSPASESNNKESGGVMVGVLTILLILYAFHCTWVTSEAYSSPSIVLSAKQHDGSRVIFDDFREAYRWIGQNTADDARIMSWWDYGYQLSAMANRTVLVDNNTWNNSHIAQVGKAFASTEEDAYKIMKDLDVDYVLVIFGGLTGYSSDDINKFLWMVRIGGSTDPSIMEENYLTDRQYRIDKGASPTMLNSLMYKLCYYRFSEVHTDYQRPAGFDRVRNVEIGYKGFGLTYLEEAFTSVHWLVRVYKVKDFENRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2E5</accession>
    <name>A0A8J4V2E5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Saposin B-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_002537</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000070">
        <property type="protein sequence ID" value="KAF2076183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2E5"/>
    <dbReference type="OrthoDB" id="69496at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.225.10">
        <property type="entry name" value="Saposin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007856">
        <property type="entry name" value="SapB_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008138">
        <property type="entry name" value="SapB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011001">
        <property type="entry name" value="Saposin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008139">
        <property type="entry name" value="SaposinB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051428">
        <property type="entry name" value="Sphingo_Act-Surfact_Prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11480:SF91">
        <property type="entry name" value="SAPOSIN B-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11480">
        <property type="entry name" value="SAPOSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05184">
        <property type="entry name" value="SapB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03489">
        <property type="entry name" value="SapB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00741">
        <property type="entry name" value="SapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47862">
        <property type="entry name" value="Saposin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50015">
        <property type="entry name" value="SAP_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035293085" description="Saposin B-type domain-containing protein" evidence="3">
        <location>
            <begin position="23"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="4">
        <location>
            <begin position="45"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2076183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="122" mass="13723" checksum="A0770495EBB1B41D" modified="2022-05-25" version="1">MAFFKSFVVLALILVCITASYSLRVSTPSHNDRVEIFDQNNNVESIPLCILCNIVVTETAKYLNNKANETEIIDGVEHDCKILHDQHDVDECTKIVNNYLPEIIDLIKENKKAVCAEIGLCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V2S1</accession>
    <name>A0A8J4V2S1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GH18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006844</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000333">
        <property type="protein sequence ID" value="KAF2071852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V2S1"/>
    <dbReference type="OrthoDB" id="3012298at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052750">
        <property type="entry name" value="GH18_Chitinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42976">
        <property type="entry name" value="BIFUNCTIONAL CHITINASE/LYSOZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42976:SF1">
        <property type="entry name" value="GH18 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="308" mass="34074" checksum="095F9D926EAB9756" modified="2022-05-25" version="1">MSGGPLCMPYVDVSLNAEWSDWQNFPEGRPNPIYSYQVNDYSLDGLVFGFISLCEGNTACWASLPQMPLDWALPLAHDIQKFNKKVIVSFGGATYKDISSHFTVDDLVNTYNEVIRMYNAYGLDFNLENPLYNIDRICEALVIVVASNPTIKISFTLQTLPQGLTSDGLALVQKAKDKNLVVTVNGMAMDFYQGETVDMGAKSIQALDSIKSQLHTLYPDESEAVCYSRTAITPMIGLNDDSSMFIIPNATSVGQYSFSKRAAFVCFWGLNRDNPSSFPHVDLSSSSNPEQKASGDYSKAFVQAIHKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-04-02" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3H9</accession>
    <name>A0A8J4V3H9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_006110</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000267">
        <property type="protein sequence ID" value="KAF2072587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6422525at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070182">
        <property type="term" value="F:DNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026171">
        <property type="entry name" value="FANCI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029312">
        <property type="entry name" value="FANCI_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029308">
        <property type="entry name" value="FANCI_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029315">
        <property type="entry name" value="FANCI_S2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029314">
        <property type="entry name" value="FANCI_S4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21818">
        <property type="entry name" value="BC025462 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21818:SF0">
        <property type="entry name" value="FANCONI ANEMIA GROUP I PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14680">
        <property type="entry name" value="FANCI_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14675">
        <property type="entry name" value="FANCI_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14676">
        <property type="entry name" value="FANCI_S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14678">
        <property type="entry name" value="FANCI_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FANCI solenoid 1" evidence="2">
        <location>
            <begin position="343"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="FANCI solenoid 2" evidence="3">
        <location>
            <begin position="609"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="domain" description="FANCI helical" evidence="5">
        <location>
            <begin position="778"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="domain" description="FANCI solenoid 4" evidence="4">
        <location>
            <begin position="1293"/>
            <end position="1533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="973"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1540"/>
            <end position="1587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="992"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1014"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1542"/>
            <end position="1557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2072587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1587" mass="181004" checksum="DD44C8F80DE0008A" modified="2022-05-25" version="1">MKKLRKQNSISGDVYNLDDHLHEPILGEPDLDYPDADEFLNSDLDEEDIRIVHTDKDKKKKKKKTSRTSFIFQLDSEEAEETDFEDNDEEEVDPEEELGEDIGDEDEDDLDLEEDDDLDGFIDEEVEEEEEEGGEEEEEDNDEDYVSETETKKKKKSKSKKDKKDKKETKKERKERKKKEKKDKKERKKKEKKSKKKKGNDGNDDNGEEEQEEEQDELNDIDSSQAVRDDGKRQKKKTKDKGVAIDMFDEKIINAYNKVKENSNNNMFGTQLNNNISMEYDELTDGLSSLYESASSETILLYLDNRIDSRKPLFDYIRAMLFAIDDNQDDDLSIKAGERAELIELLLGVIIETKKGLQEKDLTQCMELLLTEIDGLDKQFYRKVINLIAENISTVDKPQKLTLLPKCLELLSIVDPLVEEFKATTIRNISSSSWSKNLFFPIISMLSDILSLGKGNLDLIIQKVSNSMETIHKDDLPSLVNHFLMLSSHGLKGKILFIISEYFSNWEAQQEDIHLDGQILFMESLIIGELNMAMRKDMDLGKEFLKLKLKISCFNIGILLSMIKIPRFKTQAQDLLKTLTTANVKISYELTSKLFLETARMTSHGWDHILESFVYFAIHLINSNALAINETIKSLRLLGKDILFELFQLHDIIQEKVIDEIMTHIITIKSENLQSTWFLLLTRISNENPQSLGRSVPKIKESFDYLQHFSPNTVRQLISAFDPILVQYPSFMNGIFITLKKSMFARDLESRSASIIGFLQILKNLTASNSINNDGSTAVANQYKPLISEIFGIIKRSLTQQANIRTILYSGLEDLVSSCPMSCNSVCELLLSQFDQYIPDSDSNSRSPILLPRCIEIKEGNAVNIIEPFDKLVHCIQFCITKAGESEEPNIVSLCGKFSTFIESVNRADLKDYDMSVSANGSEKLASFCSILIGTLESLIEYHAVRNEPLESILKFFETYTLAKTLQLENQQQIHSNNISSSRSKKKKDKDADMDQDEDDNNNNNNNDEDDENNNNNNSNGKKGAKPTTTAKKATDSSTKKAILEQDSFSTTCLSNLFSMLSKTSLGERRNTKKFTLFLFGTTHSILQKISSQAKNQKKLLESRKHMVQSKEYYRFFNSIIPSIFHEIFSNNWVESPGSDGSKQKKQNHTQSHSIHTVALQCFDLIIQYVYDNDSYHTMNQLLSPIIKAYNDDAGEIAISNKGVMGSIEGTVKIIQNIIASNIKFESDVEIISSSLSTLCKYLVTNNQSRPAVEDLFNFIKTILQKETILSHSLACHFINLLISIERSVDFTFSKHVYVLLGAIEKDDEVNIRNAETLPIDKLPKRPSPSFNDKTISHIINLLLSVQETTMDKCKEDIIVLKKQWSPSLNEKEKDDISNLVLGICEILHSVCQVLVVLSSCKINGPPREKFLKSIKKFFLTLISLVSMITLYTMHTESLNALILLSSLLSSNTQDFMHWTDNSINENSKSTTKLSVIDKARINRESKLMPIITFTLEKYESTVIKYEKKLKITKHLGKHFKQSKSREFVIETDLLSKKLNNEDENDNNNDSDPDYDENVNSKSKKRKVAAKPKASTSKKQPATKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4B1</accession>
    <name>A0A8J4V4B1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pleckstrin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_005312</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000208">
        <property type="protein sequence ID" value="KAF2073367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4B1"/>
    <dbReference type="OrthoDB" id="18921at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051092">
        <property type="entry name" value="FYVE_RhoGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001331">
        <property type="entry name" value="GDS_CDC24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003096">
        <property type="entry name" value="SM22_calponin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673">
        <property type="entry name" value="FACIOGENITAL DYSPLASIA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673:SF262">
        <property type="entry name" value="RHOGEF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00888">
        <property type="entry name" value="SM22CALPONIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00741">
        <property type="entry name" value="DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="4">
        <location>
            <begin position="141"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="4">
        <location>
            <begin position="346"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="4">
        <location>
            <begin position="498"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="3">
        <location>
            <begin position="621"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="840"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="268"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="904"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1030"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="23"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1051"/>
            <end position="1060"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2073367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1094" mass="124960" checksum="14968A2651B39F30" modified="2022-05-25" version="1">MFSNFFGRRNTIATSKSSPPVINNNNNSNNNSNSNTSVDNSSNSSNNVQIEDNSSNSSNSNSTTSSGNNSPLSSRRTFAFQSSWNGTGASPTSSSTSLSYSFPATATSGPFNKVITAADVKPSVSSLTDDIEKKKAAKYDVNLENTARKWVEDVLEIQLDPSMTFQQLFKNGVLLCQVANKLRPGSVKRINDSSISFKQLENIQNYLKACTSMGLRDTDLFNSIDLFENKNIAIVITNIVVLGKHASKIDTYQGPHLAGEKKNVKIVTQPSPPLFNNSTSSSKPAPPSCTSKRQSLHPDTNPNDTYRNNRPLSNGKKLKWRKSVRIPAVSSLNTDIHTKEAFKYSPELQRYAQEWIESVLLEKFKFPSFSTSLKDGILLCKLINKIKPNTIVYINNGNSSFKQMENIGNYLYGCTAIGLKKTDLFDTPDLYEEKNLNLVISNIHVLANHVNRVYPQLGFPVIKSTNNSNLDNNSPLKLYSSIIHSRFGANSDNGKTKPDDYKDLLDWININLKSQYHLNLSMHVKDISEDLRNGVLILILLEHLTNQRVGIFTKEPVLPWHFMQNIYLLLNFLRDNSLSMEQVSAHDIFNGDCVTICTILRLIRDTFDRDHLYRTIPVDTRKQKVIEEIISTEQYYVKCFSLVYNSLIVPLQNSVENQKPILNSDEIFSIFGNWEHLLRFHVKLLEEFESKQSIKESVTLGDVFLDKCEFLKENYSNYINNYDNSYQRLKRLRKGNQIFDELVNTFEIFQDSHNGLDLYSYLIMPIQKIPKYLLLLKELIKYTPVSDPDYQMLINAKENMKNVADHVNESKMSVDNKRKITSIQESINNIPLNLMEVERTYIREGFLEIEDTFKKDSYFFLFSDLLLFVKYKPCEETGKEFKFKECFYLDQIVDISDIFSDDEESDVEENGNKKPSSSSSNSFEMETNDFCLTLIAESHSEKTEWMEDLRRCLQNQLLNEEDQLSDLSLASNDALLSENELEHKVLQSAFASNDENKPKLLKSVQFAVSDEEEEEEEVEVEVEVEVEVEVEVEEQQEHQEQSNVDDDEQLQEPTESITNDENNEFIEKQVEQDDNCSDTASSDYELGKLKKNNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4G8</accession>
    <name>A0A8J4V4G8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001187</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000027">
        <property type="protein sequence ID" value="KAF2077488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4G8"/>
    <dbReference type="OrthoDB" id="203908at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05276">
        <property type="entry name" value="p53_inducible_oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014189">
        <property type="entry name" value="Quinone_OxRdtase_PIG3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02824">
        <property type="entry name" value="quinone_pig3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106:SF18">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE PIG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE PIG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="3">
        <location>
            <begin position="10"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="334" mass="36133" checksum="6724B18D6B00E882" modified="2022-05-25" version="1">MKSVILKEFGGIGNLLIGKAPKPVPRSNELLVKVKSFGLNRADILQRMGRYPPPPGESDLLGLEMSGIVVEDATGTFKKGDKVLGLVGGGGYGEYCTIAASHAIPMPHHLTFEEAAAVPEAWLTAYQALFPLANYQKGDSVLIHAAASGVGTALIQLCKVSGAQTIIGTCGSDDKGDFIKSLGCTHTINYKTTESFYDSVKQITGGKGVNNVFDYVGPKYWNQNLKSLSMDGTMIIQGLLSGASVKENADIGAILSKRLNIKGSTLRNRSEEYKTDLIQKFEKQFLNLFETGALKPVVDKVFEVNDIQAAHEYLEGNHNKGKVIVRSFDFDKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4T7</accession>
    <name>A0A8J4V4T7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Leucine-rich repeat containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007558</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000410">
        <property type="protein sequence ID" value="KAF2071129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4T7"/>
    <dbReference type="OrthoDB" id="1415503at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="204"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="545" mass="62664" checksum="FED55E7865753665" modified="2022-05-25" version="1">MENNDQIFFTIFRNITLKKLIYNLLISDTTIIASPSYFDENYKFFEPLDTNNREYNIKVHIFINNENISDYISCKQKDIYNSISFYNTVSQLQQIDIITNHLPKYIRNLSLVHEEVIKKGLVENLSTIETMTCLDFTQPIFPNTLPINLEEISFSAHNNNLLMLKDVFPQSIKYISWLANTNKLDLDFVFPTNTNLHINIDVDNENYKQVIDQLNQKALEFKNNNVKIKRLVLHYEANEQPFTPNFFPPTLEELYIYTFRDSLPPLANALFPDTLTRLKIRNLESKIQVGDLPANIRDLDLCSYGYPLTVGLLPQSLTKLKISEYNQPLEANVLPQSLKTLRLDGFNQILDCNILPCSLKKLILSNNTTLRQGCLPHGLESLSTIPETIKGFTSFPSTLTTLHLNHCKKRNMISIVPILPSTIHTFSLTLREGMAVGALQRLPTSIKNLSIYVRISNEIDRIDFLPNNLQKLSLECAYAIKFLTFTDPHTKPIVCDDRVPSSLRLLRITSHNRMFYTPPSFVTTLIVNEYLESIQHLPSPNVLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5H0</accession>
    <name>A0A8J4V5H0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_006766</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000327">
        <property type="protein sequence ID" value="KAF2071909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5H0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="109"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="503" mass="57748" checksum="4F98B7A5F223CBDF" modified="2022-05-25" version="1">MMDTLGSNNITNNSNNNNLFYSVWRNIFLRDLVRVKVFVNTKKRISFDDLETNNPLVSLTNEDKLKHNIFLESFIFDQEDFENYVKNPYRHVLNSIHFNSSVYTPPQQYDDEEEEAQEQEEEQGGVEEIKELDLQCIHQGVHCVSFDIKTNSKPCQGFLPDSVTQVSISEMDGECNTQFINSIIHSLPSGLKSLSIPEEYKFTSPYVMNDTLHTLQYRSTHDSMQWLVRSPNKVLDACTLEIKAKEEYAWLGNNKWITSAAVIRSMGDLCSNIVPQHVVKLSIEYDDAVIQPDAFPKKLELLKCTHPIRFDKNVLPYSNLTHITLKYYFEKLEKDILPCSLEHLKIGHFEQPLDQVGVFPQGLKTLVLKKFDHPFHPFVLPQGLQILNMPHFKQPFIQANVLPPSLLHLEMQLFSGSFESGSAPLDNLQLLTVDALDASISLLLTNVVKLRIWTLNGFKKGTSLYNTSIQDLFLYSYERVSVNQSSLPQHLKKFRADGLDIDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V617</accession>
    <name>A0A8J4V617_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000001">
        <property type="protein sequence ID" value="KAF2078673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V617"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035184408" evidence="1">
        <location>
            <begin position="19"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="316" mass="35211" checksum="D2DD1DDD92F178CE" modified="2022-05-25" version="1">MKSLSLFLLVCTLSLVIAQGPNPPVTFINGYGNIGSSSILTIDLTMNFQFKKPLNPIPPYMYGNVGYFPFSNLLSVIYANSSSSISWAYLNYKNGYLAESETFEISTSYKESLINGVALFDQKTFYFLGTNDRNQVDLVTTSFGPSSSSQVSLPITLSSGPTQGALDNQGSNYFVVSIINKNQYECTWVNTTENQVIKTIKISPSSLAPDTKFQIYAYNKDLYIVQCDPKGQYVQIYRVDWSGNSVELVYHELVANPPDGLQISLTEKFVAIFTYSLDRYNPSNVAGSLIKLSSFSKYQQLNALPYPKNGDKLFIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V635</accession>
    <name>A0A8J4V635_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Extradiol ring-cleavage dioxygenase class III enzyme subunit B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_006279</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DODA-type extradiol aromatic ring-opening dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000283">
        <property type="protein sequence ID" value="KAF2072404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V635"/>
    <dbReference type="OrthoDB" id="7396853at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07363">
        <property type="entry name" value="45_DOPA_Dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.830.10">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014436">
        <property type="entry name" value="Extradiol_dOase_DODA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004183">
        <property type="entry name" value="Xdiol_dOase_suB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30096">
        <property type="entry name" value="4,5-DOPA DIOXYGENASE EXTRADIOL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30096:SF0">
        <property type="entry name" value="4,5-DOPA DIOXYGENASE EXTRADIOL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02900">
        <property type="entry name" value="LigB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006157">
        <property type="entry name" value="Doxgns_DODA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53213">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Extradiol ring-cleavage dioxygenase class III enzyme subunit B" evidence="6">
        <location>
            <begin position="13"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2072404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="264" mass="29356" checksum="C6E2CFA67F418683" modified="2022-05-25" version="1">MTNNILKMPSLFIAHGSPMLAIQTTPYTKFLNTLGDSLKPKAIVIFSAHHETEVTTVSVHDNTYDTIYDFYGFPKEMYQIKYPAKGSSSVANSVLDKLQKSGLKAQKETKRGLDHGSWTLLMKMFPNADVPVVQVSVNPNGDARQHYEIGKSLRGLKDEGILIIGSGVTVHNLGAIKWGQDKPEPWAVKFDDFLIENVKSNQLDNLFNWSKLAPNARLAVPTAEHFFPFFVALGASSSDDISSSKVINRHYEVGTLSYLSFNFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V788</accession>
    <name>A0A8J4V788_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004982</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000188">
        <property type="protein sequence ID" value="KAF2073694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V788"/>
    <dbReference type="OrthoDB" id="16239at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="144"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="424" mass="45169" checksum="0C877C8F69633F96" modified="2022-05-25" version="1" fragment="single">FAGTYSITSSSISTTGSSSTSTSTSSSSSTTQGTVTSTSTSSTSTSTTTGGSVVTSTSSTTSTSTSSTGSTTGSSSSTCTNFCPIPAPSFSYSFTSPADPSPAFPTPQYFASGDTGVSYFPNYDGLKLHIRCYSNPSLTTGNTVILDSGLPFMSTAWGGFIQALIPQMTSLSVKRVCAFDRHGYGWSDPAPVAFDMYENVRRLRGALKMLNFCPPYVLGGWSWGSIGVQTYAAHWPSEVKGMFTIDGSDVGVLTDPAWTFSLPFLQDLFSTYITENNNGTLASNTASISNFYGWMIDIPSVPAPCVSNSHTLFLHPQNRYLTATKQELSVFMQSADHLKQTYLDSGLTYPLRNIPFVVFTATSGGTDWTNRQNTLASLSSQSQHILNDVVDHLVPLKIPSIIVDVYTALNTLMSTSSPVTATCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V944</accession>
    <name>A0A8J4V944_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000005">
        <property type="protein sequence ID" value="KAF2078407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V944"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="282" mass="33363" checksum="C9BDA7DAEBCC99E8" modified="2022-05-25" version="1">MMEFMIQKSKNNSKKQKNGTFGRNSKWTEKNINRRELLNFNNHCNILCLYESLHKNNIPLDNVATIEKKLIKKGRKYLNQHYQNISGSPTNNYSNNKKICKTKPSPQSIKKSLEKIQNLELILNADYLGMNTPSVQDLNLVNYNFKFNLSSIRYILNRVLENKEIQIELWGIPTEKSKDKLEELRKNLIHNFGYNYPINQFKFYLTTFLLHPKESPFAKEKNFIRAQREPEFKNQFQLKFNSITRLANEMTTTTIPGPPNQLERDENESDFDGDLYNYCFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V9K3</accession>
    <name>A0A8J4V9K3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">CYY_002499</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Plastid</location>
            <location evidence="16">Chloroplast outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000069">
        <property type="protein sequence ID" value="KAF2076199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V9K3"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00257">
        <property type="entry name" value="beta-trefoil_FSCN-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008999">
        <property type="entry name" value="Actin-crosslinking"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF135">
        <property type="entry name" value="TRANSLOCASE OF CHLOROPLAST 120, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50405">
        <property type="entry name" value="Actin-crosslinking proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="10" id="KW-1002">Plastid outer membrane</keyword>
    <keyword evidence="12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AIG1-type G" evidence="18">
        <location>
            <begin position="3"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="199"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF2076199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="401" mass="46038" checksum="C044B73FEE2ADF9D" modified="2022-05-25" version="1">MTRTILIIGRTGSGKSTLCNIITSTNSFRESEYGVSETKQYKVVSFSHQGVLYNIMDTVGLGDTKMGELQILDQMVSAFKGITDNLSQIIYVFSGKFTQDEINAYNLLNLVFGQDVSRITTIVRTRFPKFTDTEACNTDKQLIINENNQTNAIVSRCNKFIHVNNMTEDEQPTLKNRVDAKTILLVHLQGCTTAIPTNEQAMRNNINSHYQRIEEEKRRQQARELQLAEQQRVLAEQQRLAEIHRRELEAKILAEKRLRGSDFWNRNIVIQNWHGQYMTARAPLVGVDLYSKHRGPSQIFTLVPVEGYKVAIRNENGLYLRAHPGGQGARLDLSNNLLDWEKFILVQHGDRWGILSFHGTYIRSHRIRDSFDRDGAPTISYGHVDLQVHMKEWELHKLIPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V9M2</accession>
    <name>A0A8J4V9M2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002437</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000067">
        <property type="protein sequence ID" value="KAF2076259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V9M2"/>
    <dbReference type="OrthoDB" id="5834362at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070176">
        <property type="term" value="C:DRM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018737">
        <property type="entry name" value="DREAM_LIN52"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10044">
        <property type="entry name" value="LIN52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="120" mass="13380" checksum="5E19C98C3CDBCB9B" modified="2022-05-25" version="1">MDNNSNTTTNNNNNNNNNNSHLNTTNDFNINLSQINSTLVDFLEPLSKDGLSSGRFENLSKLSVTEIRDQIKRLEEWAYVLGVQENKHLQTGKLLGILTSPPSDLNLDGGSNHQNNNNNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VB09</accession>
    <name>A0A8J4VB09_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_000961</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000021">
        <property type="protein sequence ID" value="KAF2077714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VB09"/>
    <dbReference type="OrthoDB" id="3784at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00761">
        <property type="entry name" value="Glyco_tranf_GTA_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12526">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12526:SF630">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="5">
        <location>
            <begin position="185"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="4">
        <location>
            <begin position="649"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="868"/>
            <end position="954"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2077714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="970" mass="112473" checksum="5C6F597D1FA9BDAD" modified="2022-05-25" version="1">MNSSNNYNYSYSLSEDEDENIMSYQYHNANNNSSQYSYSSSKNNIIDDEGFIQEDNHIKYSKKNKRSIWNRISYPSNCSKRKGYSIVISLFLCLSFILILFSFILWSIGNNDKAVIDSYCRKASYRSNSDNDDAIDNSGGDYCVFDSKSFEPDYSCTPSFINRSMQYQYNSRYGSSDQFTPPIVSVVIPFYNTPVDQFNECMISVLDRQSLQNIQVIVVDDGSSLQNSIQSLHQWKEKDKRIRIITHKANRGLPASRNTGIANSIGKYIFILDSDDLIEPTCLEKLVWKLESSPHIHFTKGYTVGFQAINYTWQVGFEQRSKFLNENYVTVAIMFRAELFQIEGGKSKSIAFGYDESIVDGMEDWDFYLKCAEHGYWGETVPEYFDWYRRKPSSAQSEQWKNNQGVKKQLFINQLKTKYTKLYKDHYFPNHSATPNPFSLQVNHSIFSNHLIKHKPRVLLILPWLNTGGSDKFNLNLCKQLVEKGWEITIITTLKSTNNWLNRFQYYTTDIFLTSNFLTLDNLPRFFCYIIESRKIDITFLSNTDLGYSFLPYLHQYCPSTAIVDYNHMEEENWRNGGYPRYSLSMQPYLDLSIVSSNHLKQWMQSRQQQQQQQIDNDKIQVSFINVDPKEFEMNKSFRYEIRNLYHIKPKQHVVILYVARLVPIKQPIEALSIFRNINDQLADSSGPKFSVLFVGDGPLRNQVDQYVQSYGLNGVVKILGDVPTQDMKKIISASDIVFVPSLMEGISMIFYEAMAAGVVPVGSDIGGQDELVDKNCGYLIDPSQYQSTLIRLINDPQHLSVLGKNCVERIQSNFGLDKMGDNIISLFCKSYYNNFSKQQRQQQYQQQKTGSIDSNIGVEMAIQAIELHKLQSKNEIVEQELESLQKSMENDRAIHEEFEREKLKLVKLQKELKSVVYENEKIQQEYSTYVKENENLFNKYEDILEDLKECKRTIDINNNGSSASIKINI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VBY5</accession>
    <name>A0A8J4VBY5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SIR2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_000045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000001">
        <property type="protein sequence ID" value="KAF2078674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VBY5"/>
    <dbReference type="OrthoDB" id="153872at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46563">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46563:SF1">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13289">
        <property type="entry name" value="SIR2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1027"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="83"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="341"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1031"/>
            <end position="1042"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2078674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1142" mass="128540" checksum="568E20F18A87C35F" modified="2022-05-25" version="1">MENNKNILNKDNADDLSAKGERVAPIPTIPIGNDHSTTNNSNSNTVTTTTTISSTTVNTLDISKEFDKITIKDESVNTTYGPTTTTTTTTTTTAAAPNKRSISTASENISYYSQNNNDNNNDNNNNNNNNNNNIIKHHHQQEEYESDKEESDQEYDDEQDDSLLSEISSVTSAPENNNSGRLDEDDNEDEGNSNNNNNSSRPIKIGASNGKLKLSVSANSMPTAPAKKRIKYFPLTQHLGKLDQIKKVQEIENDPNELTRKEIKSMFEEDKSVLLPTSPEKRTVEEINSSGDDVFSTDFKPLFSSLIDDTTTNGNLSSIPPIIPNPFSFTATTTTNTKEIITTTITTPTPATTTSTSPSTTITPTPTATIGDKKEEDKEKTDVDKEKEDKDKEKTDADKDKEKESVKVSEQTTSKAEEGKESVLASFFNNNTVSTEKDKQEKPTAEEIDNELKTVSIDIGKLVNSTTIPTPSTTSAPIVSPDVVNIKETSQSIKQEKTKIYTNRPKKVDVKNEYFKYIEESINEEVNFQPSTLPKESDDDGHLFLLYGDLTKLVCDVKMVPCSHHSIFRVALSSWLKYDWAQFPIDIRKKFNNTTIDFKANQRRVCKLESWPIEYPNISQPWFCNVVPFSYLAGPSWYIKGAKEFLDSVGKDLLLKKPPIKNGRSKYLVSLPILGTGGGGGSGMAGEILTLLIHELFVATRVWKYDIVLVTNEISMYTAARNKRMELMESDRTFSHLYRTLLGQELMNKAHVLSKMMDQGKLAIFIGAGASRSAGLPTWAGLLQMLGDKLGMTEEEIRSMEQLHYLDRATVLESRWKKALAKYSPPEQKVQDREEIKEIFEIHKTIYNEINVPMQTEIANLMKVSHSGLPHFLLASTPVQEIITTNYDECFEIASKSIGQSICVLPYQSLNSHDKRWILKLHGCVSNPQDIVITREDYIRYGDKKEALSGILQASLITKHLLFVGFSLVDDNFYNVMSAVKKVTTSKNRKKKFGTALFLQKNDLMNELWGQELDILCFESTVQKDQKDQKPSTVNNATSQQHKPLPNDIANCARRQEIFLEYLSNICISNTSHLMEKRFDCLLKDGEKIFRDRILEFVENLPQEAKDTSVYLKFQKFLSSIGFDKNNVRLRKSCIIVNHEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJ38</accession>
    <name>A0A8J4PJ38_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PKS/mFAS DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_010594</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001225">
        <property type="protein sequence ID" value="KAF2068080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJ38"/>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.110">
        <property type="entry name" value="Polyketide synthase dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042104">
        <property type="entry name" value="PKS_dehydratase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049551">
        <property type="entry name" value="PKS_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049900">
        <property type="entry name" value="PKS_mFAS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050444">
        <property type="entry name" value="Polyketide_Synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45681:SF6">
        <property type="entry name" value="POLYKETIDE SYNTHASE 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45681">
        <property type="entry name" value="POLYKETIDE SYNTHASE 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14765">
        <property type="entry name" value="PS-DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52019">
        <property type="entry name" value="PKS_MFAS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PKS/mFAS DH" evidence="5">
        <location>
            <begin position="206"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal hotdog fold" evidence="4">
        <location>
            <begin position="206"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal hotdog fold" evidence="4">
        <location>
            <begin position="340"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for dehydratase activity" evidence="4">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for dehydratase activity" evidence="4">
        <location>
            <position position="404"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS52019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2068080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="675" mass="78582" checksum="FC562BE2783A2CEA" modified="2022-05-25" version="1" fragment="single">DKVFAAFLGTRASFHSSSQEMIKDELFENISNNLFNINNNCNGKPIMFSTTTSNEIKEYSNQLLFDNIRNPVYFEQTLDNINNYIVENNNNISNSIIFIEIAPHPTLSRSIKDYYDRSENKNIKIYNPLNKKTSQTEDQLFKQTISNLYINGYNIDFSCQFNQDQKMDQSYKHKCQYLFPHYQWDDQIYLKESERMHKFRIEGPPVTCLGNLVYENQKSKSYKSIINISREPFQYLKGHYAAGKNYFPGCGYIDNIIKALSQSDNLVISQLNFSIPFILKPNVDFELQSNFTQTSKNEYKVDFNFYENGKWNNSACALVHLSPAFKNQKIDFQSIKSKCNLSTVLESDLYNLITHKADLTYTDHFARVEELYIGNDCSLSKIAMKPRISKSCDNDYFFNPAILDSCLHGLFPLIKNPLKIIFDRISDMKIHTENIKKLNIGDIDYIYAYSSNPNFDKVNNRASFTFNVMLEDGTLLFEIQQAVCASLTPIKDISAKNPQENLYTLFWQPKEAIIKNILLDPVAEPETIDQTHYKQFCTMLFYNQIRKKSPSTLTKSMIMDSSIEQLIDRYLVDPKVKSKYSKLFRLAFEVLQQTPDYMIDDTNESFNEKLEQLITIVSQDLNCCELIKKSTRVMAAMLFPNDNIEYEDTQQYLFQDGGIHHVYDNPQITEIVEWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJA5</accession>
    <name>A0A8J4PJA5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010294</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001022">
        <property type="protein sequence ID" value="KAF2068380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJA5"/>
    <dbReference type="OrthoDB" id="21613at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="329"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="592"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="125"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="300"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="329"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="394"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="592"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="614" mass="67655" checksum="511BC8F96AA7A336" modified="2022-05-25" version="1">MTTIDQKSSLTSSPTKSLDVLSSSGSSSSSEGNNNNNNNTEFKKLKLSPLPSIHDCFNSPPPQQTPLSLFENPSKLLKEEEMIESEFTHHSNLKNNSNNNNNNNNNTPIKMEIQQSIPSIVNMTNNNNNNPPSPSNQSNSPNHNLINSPSSHLTSLGGTSMSTTTTTTTSNTIALVKSTSSNSLSSPPSPAITSSAPSTPLSDSNPGIGGVVPVHNPKISPQTGLQTCSHPNHELWWQTSPELPVSEFYSRSYKCKKCYIKQQTESKKTRQNHHPPNNVNNQNQNQSPVNSNNGNHLNSKDNISNNLNNGNSSNNNMINFMGNINSTLSNSLNNNNNNNNNNNNNSNSNNNNNNNGSMLNSLANLSQIFTNSNPQSSTTAPYNNSPPRQQIKLNNNGNNNNNNNGNNNNNNNNNNNNKPKYTKKKSQEDIYNGNNNHINSLLSKNNPNLEEQEKWNQLHHPINTPNTFIEQAFNKNSMKMNNGKQQQQMDGFETDEKKIFDFQHALSQSQSYIRNNLNPNSKTFIDSYLSGTLEDTDLKSTFRIIGDIEGSICGGFITEVMVGGKTFKGVVFEDMKGSNSLNSNSNSSLFSLQQQQQKGISQNNNELNMNGVYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJM1</accession>
    <name>A0A8J4PJM1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_010400</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001087">
        <property type="protein sequence ID" value="KAF2068274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJM1"/>
    <dbReference type="OrthoDB" id="21495at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050460">
        <property type="entry name" value="Distal-less_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24327">
        <property type="entry name" value="HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4 5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="7">
        <location>
            <begin position="311"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="313"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="175"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="367"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="37"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="100"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="175"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="199"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="217"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="303"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="375"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="404"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="421"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="502"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="518"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="542"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="589"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="630"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="658"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2068274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="707" mass="79235" checksum="CBEAA6B15B9BAD6B" modified="2022-05-25" version="1" fragment="single">MNIGSLLQSDSEGKSEKNSNNGAIGSGSIFQNGGVENNQPSSSSSPSPLNSPSPNQFSSTSSSLLSSPSFNSHQQQQIHNNNNFSSDTLYNQHQHHQQQNRNSPSLSPPSKKTQNNKKVRLESEIQQQQQQQFTFSQYQPNHSNNNNNNQLSSSLPGIVVSTLNLSSAQNTPLNCNNSLHVSQPNMPPLHLQTQSPNLRSPPLPRSPSPPTQLYDRNNNNSFNNSYNNNLNNNNNYNNFNNSYNNFNNNHNNLNSNSYNNNNNNSYNNNSNRFNLQNSSPTNSPSSSPMTLCGPGGAGGAGAEHSRSSSPEKLKKRRTRLKKDQARILKEFFDRDDYPTKEDKEQLAKELGMPYGAITTWFSNKRQEVKRKMGASQESSSARKGTSSPDRGSPAVNRFRDERSNLTPMLNSMTVVRSPKINNNNNNNNNYNNNNNSSQQFHQSHQYHQLQQHPQQSIVQQSNVPPLSLVSPRSSNENLSIKQKTKVQKYNKTTSSQSKGSDSESDDMDEDQDDYKLKSNNNNNTTTTTTSTNSTNIRPSFDSDSDDEDAAFFYEEDDDSDDSDLNNNNSKKHSSSQQQPQQSFVDFKSIHNNNHIHNNNNNNNNNGSSTLPNSRHKRRNSIPGDFRYIGSVSANNNNKISNNNNSLFDKKRKSPEPDNSNMENNNDNDTTMCNINNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJX9</accession>
    <name>A0A8J4PJX9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009537</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000734">
        <property type="protein sequence ID" value="KAF2069145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJX9"/>
    <dbReference type="OrthoDB" id="23871at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="438" mass="51272" checksum="9DC3A8103A2A1C0A" modified="2022-05-25" version="1">MGNLKRKSQEIHTFQQINPTQLDDWNACIGKDEQEFINTWNRLENRISFINGRIFYIKNLDQVADNNVMGIDAYLFDILSQVCKSLQLVHFVMSKGFSRICDIKAPVKYTICLLTLKCHYQDISQKDRDLIQSIAVNDFKFVSMVLTNCHLLGHKENFPFFFSLFTQGLDARKINVMVRILFERATKDRDVFLYESLEGIGYQFSMFNNDSTYYYGFKSMLPHFGKMIDSFPQEQKDQGCCQMLVSLIYSNTNYFGFKYLFTKHKFSPLMDTAVIQAFADCNNLAFIDYLWMNRSTCFIETSSPTDINTFFTTLFNHASTTPNMALLEYLIKNRCIYKTDYKNLTQKPYFGCSEPIDPKYNLDVLVHCIDNRFFDSIDPYVNYLLDPDSECSTALVEYIYRNQDKFNTEISFQSIYDQIKTSGSKHLQNHLLELIEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKE5</accession>
    <name>A0A8J4PKE5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010224</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000981">
        <property type="protein sequence ID" value="KAF2068448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKE5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="94" mass="10171" checksum="5905A8EA81AD92ED" modified="2022-05-25" version="1" fragment="single">MQRGFMSTVQLARSCTRSANSMKSSFTKVSVATSTTTNTSNIYKTQTSSSSSPCTLNKSTSTPLSSFNNTSTNRISTILGSQQYTFNNFLVISD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKG1</accession>
    <name>A0A8J4PKG1_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001205">
        <property type="protein sequence ID" value="KAF2068104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKG1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="342" mass="40285" checksum="9C346EDAF7070490" modified="2022-05-25" version="1" fragment="single">CKLDNTIEYKQQLKKLNSRLHDIVTLWDKQNSVAIGLINQFIEEEEEKEEKLGKSSIDGPKHRYEIKEIDGIKRIVKPEINEFLYEAYLQMAYGYIKSYPKLAFQTFRKCQQIDPNAHLWYVEIAEEVKDFVDKQELLDFLDTFTKQVDNIVGPRFNFIKSVLLYDLGRYEDSCAHALRLSHLFTDQNIPPSYKSYVIFHCVQSILAYIEELDKKKPSTYRPELDPLNHLNVQEVLLLRDHLSNPPTDKEELSKGITTLLPRLIKMSQENNPNAPEHPRDDPAYSAFEFKTTLTLFLHTGILLQNNKESQEKEYEYNISSEKKLNLFFKAYNYMIKQIFNDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKH7</accession>
    <name>A0A8J4PKH7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010026</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000890">
        <property type="protein sequence ID" value="KAF2068647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKH7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="57" mass="6649" checksum="14CF902D3CDF3635" modified="2022-05-25" version="1" fragment="single">MDNDNSPQYMEGSKKRKHLSPIDAFQGIIQGHLQLLDQEIKDLERRRDTLLDVTKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL73</accession>
    <name>A0A8J4PL73_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_008699</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000562">
        <property type="protein sequence ID" value="KAF2069985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057709">
        <property type="entry name" value="DUF7949"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25820">
        <property type="entry name" value="DUF7949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1366"/>
            <end position="1389"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="5">
        <location>
            <begin position="649"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="4">
        <location>
            <begin position="797"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7949" evidence="6">
        <location>
            <begin position="1066"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="3">
        <location>
            <begin position="1117"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1007"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1009"/>
            <end position="1065"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2069985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1407" mass="157243" checksum="61255D8092242BC1" modified="2022-05-25" version="1">MINNTSNTITSIWVTTQTFIIEIPPNELILKGVYIFKDVCEYNIDFSFDQTISATVGTTYTSFGQTQEGRDLYTFKYSRPQGSGALDTEVTLLNGTVFQLNTQFEYTCIPVPFPLELVDDIDKKIMIFDDISTVASVIEFKNNVSNSLLPISISCTVSCTTSQLSNSRQRYMLSFSPQNWKVVDYEKLNDITVSVIDSFNRESKFSYSLFLNSEPCTTVGGLSIIPKSASIPFSTPSLDHLYQFKVVNNICNNFYFFQVVGVSTGVFSKPVAGSSVNSTYMIAYNYRGTPMSSMGDTDINGYLITRDGSSLVSSFQMFVPSQPTIDFEIVSTDYYNSTNPDTPLVSCIGKYNSYVSPDSFQKVERFDFFSFSTRFEFPWGYSKGNIQSCDFSVSFPLNIWARDYYYIDINYSVTNAIIPRNHVDFYTPQLGTYSYKKLSTRLILVTMNITNQVSPFLMGHQDTDIFFSFANLVNGTLNEGTYQFVIDLSQQIFQSISLTNRAQMKNTVSPISPFTSFHYIPNLFDIKNFTNAQMQHQYADISGLSSPFQNKFYFNVTDPHPDAVFLFYYQTNVPFYLNAFPTYHDSLCYWTGDDSKHHYTCDFKVVPNLFTNTYQFYIYLFNENPSIVTSAEINLLFPNSTFNFESIDADEIPPMVSSIFIDNNVVDFTSGSFTEKTVTVQLNITDSVNGLKEGYVIFTSEYDAFGYNFSLNPSLSTNNDIYNPSFDLKLNLTNNCISQTYTITDILLIDKFDHKSTYQSHRLLSLAIEFVNPIYYIEPKASLQIICSTDYPDTIVPVLTNYQIKNVNPIDVGVWNRENVEVEFTVTDSESGIMVDYDHSPMVYLDDFHSIKRIKQRCELKSKTVTGSIVTSATFTCSAPVPHTFGAVNGIAVSLYGIMDNYMNFVGFSSLDLEKLFGHSIVQTQFTQTTPIITKGYAINEFEILLVGYNFYSGTDTIVEIQDMNGNFIQQTQTNYIENVVLSVTVPQTTMNTLTFRVTRFNQQSNEFSVKTSKAPPSSSSSSSDNEVSFGSSASASLSTSSERSESTQSSLSSHTVPSSPSPSLCQGEPVCGGPDRGVCTPSGCKCTYPAIGEDCSSVVIVVPVPNINYTSPSTEQTIDKTTIDGKPIKYSSLVSVYSIQEIDGLTNKVVKEDVFSQWILSDISTSTSPHHYLYQSNFTHNDPAISGASLTTSINVTIQWFTNSSEIQFGSKMLSINANSMKYNIQIDKYPFKSQLNILHIVMKAQLGSDQTADMCSDTRSGNTTDNNQYIQLQVNDHSLYGRFLKTAIIDGEETVISNKIDVDGQQQQSSSMSAATVSILVPYFRKIAVIDPDFSLLLDSKPALDQENSVCSSSTPSSLTKAQLAGIIIGSVGFVAVATTCIVYYFYKRKQGLKLAKKLSDLLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLN4</accession>
    <name>A0A8J4PLN4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable cytosolic iron-sulfur protein assembly protein CIAO1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_009470</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Essential component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for the maturation of extramitochondrial Fe/S proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat CIA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000717">
        <property type="protein sequence ID" value="KAF2069213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLN4"/>
    <dbReference type="OrthoDB" id="284782at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000136">
        <property type="entry name" value="Probable cytosolic iron-sulfur protein assembly protein CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03037">
        <property type="entry name" value="ciao1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028608">
        <property type="entry name" value="CIAO1/Cia1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="55"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="104"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="149"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="193"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="292"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2069213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="328" mass="37166" checksum="26D4E3E60318F4E5" modified="2022-05-25" version="1">MPLELIGSISDGLCPERIWHVAWHPSGDILASCGNDKQIQIWAKEQNKWVLAQSLEGHEQTVRRIAWSPNGKYLAAASFDVTTSIWENTSSDPQDIEFTHVSSLEGHNYEVKSVAWDSTGTLLATCSRDKSIWIWEMQEDNEFECLSINSGHSQDIKCVAWHPTLELLASGSYDDTIKFWKDIDGDWECLYTLSGHTSTVWDISFNSTGDRLVSCSDDRSLLFWKYFEGADKWKNVCKLVDHSSRPIFSIDWSHLNDKICTGSGDDSISILEQDKSASTADSEQYKVTIKHEKAHNSDVNCTRWNPKYPNIFASCGDDGTIKIWKVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLU8</accession>
    <name>A0A8J4PLU8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_008318</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDPK1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000502">
        <property type="protein sequence ID" value="KAF2070362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLU8"/>
    <dbReference type="OrthoDB" id="347657at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01262">
        <property type="entry name" value="PH_PDK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05581">
        <property type="entry name" value="STKc_PDK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000191">
        <property type="entry name" value="3-phosphoinositide-dependent protein kinase 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033931">
        <property type="entry name" value="PDK1-typ_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039046">
        <property type="entry name" value="PDPK1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF163">
        <property type="entry name" value="3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14593">
        <property type="entry name" value="PH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="90"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="243"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="450"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="40"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="243"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="472"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2070362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="622" mass="68109" checksum="2C21B44BBF64AAF5" modified="2022-05-25" version="1">MDTHITNNSSSSSDNSSNSNNSNNSSSNSTQQLPQHQHLSPVLTSMSSSSNSSSSVLSPNKIDIHLSPFSVSPMNSSPSIQPKKKTIDDFFIGRVLGEGSYGAVVMGTDKDTNVQYAIKILEKKHIIKENKIKYVQIEKEIFCKSNHPNIVKLFFTFRSDSCLYYVLELCPQGDLLHQLKKVGAFDYTSTQYYTAEIISALEHLHGLGIVHRDLKPENILMSADMHIKLTDFGTGKILDQNTLNNNNNNNNNSAVSSLSNSLNSANSTNTSLNNLSLNENNTTTTTTTTSGTDGNQISNIALAAAAATTAAMSSGPPLMATTDMNNIQNLGALPTRANSFVGTAEYVSPELISNKETSTDSDLWALGCIIYQLSTGRLPFRGKTEFLTFQKVSNRDLVYPKNMNPVVKDLIEKLLVLRPSERLGSLSAGGFENLKKHPFFAGLEWDSLSSKTPPPIKPPADKPAFDDETPDLSSSNNNNGLRSSNNNNSTSSSNSSSNLSSSPVSLNSPSTNSQNHINDKYSKFLEPNESVVKQGLVWKKKGLSIKQRQLILTSSPRLIYVDPKKVQLKGQIPWSDSIKPEIRGSTNFVIYTPKRKYLLEDVDGAPQKWVDAIQSVMTKIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLW4</accession>
    <name>A0A8J4PLW4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008291</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000498">
        <property type="protein sequence ID" value="KAF2070392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLW4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="100"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="61"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="128" mass="14886" checksum="666BFAC5BF9B5B72" modified="2022-05-25" version="1">MTVNIQDPDIETRVKDRLTTSLGRCIICNEPIVMMLHYYKCTKMVNNIVTEYLEEYKDDFLIKQITEEDKLKQKVELMEKENQELKEKLKKMEALLKASNNPNSSLRITTPSKSTTRPFVTSGKTVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM39</accession>
    <name>A0A8J4PM39_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009105</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000637">
        <property type="protein sequence ID" value="KAF2069579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM39"/>
    <dbReference type="OrthoDB" id="24485at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="576" mass="68913" checksum="8F7EB91D1155834B" modified="2022-05-25" version="1">MKIRDIEIIVEGKNFYPGSYTQDTRLKEEDDHEVKQHQIKEHSRNNSADLFMHIYRNITIKRHIFSFIHRDLVPFYKYSDIHSLRWILNYGSTQLFVDKVKRNEYLSDILYLSNRLNTFKSINDIEFYTIFKHRYRRYLLDTTMTNVRTFLDTIFNNNKDNNIIENINDNSNKQPIDINELGVKALIEDFKNNREKTAYYLKRALESKHFLLAKYVVDEWLVNNNENDKQTILNDLKGYVLYMVDKEIIQYFCNNIETNNRNHLTIGIEGILYHDDFEEIMKLLEKSGMVNKNDTKYSQNHSKTKRVMKYFIENGYKYNLLFTSIFSTITYGTDDQFAGLSLVLDHFEYTDHDLDCLVNYSDRKRSHLIFGEIFKRFTIDQVREQYPKTYENYQYYKSMYNEFYSPNPKSSDNQQSLPPQEQYQIQKTVTYLCRGGVMSCIRRLVGKYQNDSNMLTIIFNEIFRHSHLHVLKMLVNEYRHVIDGYFSQTKINDLFTLLGTGKQESVSLEIFDFLIDKLRFLPSKYFFKLDKIQNILLASYFACKVPPQQQTPCISKEDIIDYQKKSFLPPLTLYKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM58</accession>
    <name>A0A8J4PM58_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008092</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000471">
        <property type="protein sequence ID" value="KAF2070587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM58"/>
    <dbReference type="OrthoDB" id="498371at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052050">
        <property type="entry name" value="SecEffector_AnkRepeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586:SF3">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="745" mass="87483" checksum="62D5CFE8B3872117" modified="2022-05-25" version="1">MEDSVTLCFFQIFHQSYLKKNIFEKIKEINQYKKTYKYDSIISFDWMIENGYHNLLKYKLRRNDCYIHFSLNPDLIFTLKDRELFELSFKRFSNYFPPHYPLLITTAARFNNHDAIRYLADDSLHYPRSYRGSVNSQALMYITLHKGEHVLPMVKFMVATFNIPVVEGTLKNAIRNNDYELCRYLISIINKYEHEFRVEMLLTWTCSYGDLKLFKLIYENFTQPINEAILQSATNNAPVFIYLFELANITNEKTHWIFKPSTTIMLANVQLDSPSIIENLLSSAIMNGNLEIVKYIHGKNFLISIDRILNTLVKLSLNSGHLDVFIYFESLFNSARSSINDMFLCDIKNVSNQLESVVLFHETFQLKIKPDCLCQSLQSTDIRVFHYLFSKGDFQILNSKIFFRSTVDITHNIIKEACSCYNLQVLEYLLQSGVKHSSTLQMGEINWRELGSRDENLRLIQVVLEICTPVRQYLLKMMEQAGYFGQTKIFKYLYLNFLSDSVQDTTEFRTVFSLILSKKRIEFVKFLLQNGEKVPQFYIGEYELLGIAKDVQDPNINYSQLLTQATSFGNLDVLKKFLPMASINLEELLIPALDNGHYDCTMYIINELGYKPSTYKLIQKGLENRNYLIAQYFYHHHFQDFKFKIQQLFDHFILTGNLDSIAFYHNLGCSPTVANITFLLNRQIYWVLEYFRGKVDKHLLSKIDVKKLSYPSNDHYTIYVKNYLVQYSPATPSKNSFFNFNCIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMQ3</accession>
    <name>A0A8J4PMQ3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">rRNA adenine N(6)-methyltransferase</fullName>
            <ecNumber evidence="9">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_008955</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>adenosine(1779)/adenosine(1780) in 18S rRNA + 4 S-adenosyl-L-methionine = N(6)-dimethyladenosine(1779)/N(6)-dimethyladenosine(1780) in 18S rRNA + 4 S-adenosyl-L-homocysteine + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42780"/>
            <dbReference type="Rhea" id="RHEA-COMP:10234"/>
            <dbReference type="Rhea" id="RHEA-COMP:10236"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74493"/>
            <dbReference type="EC" id="2.1.1.183"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7 8 9">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="9"/>
    <dbReference type="EMBL" id="AJWJ01000606">
        <property type="protein sequence ID" value="KAF2069725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMQ3"/>
    <dbReference type="OrthoDB" id="74991at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052909">
        <property type="term" value="F:18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.480:FF:000002">
        <property type="entry name" value="rRNA adenine N(6)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000007">
        <property type="entry name" value="rRNA adenine N(6)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00607">
        <property type="entry name" value="16SrRNA_methyltr_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011530">
        <property type="entry name" value="rRNA_adenine_dimethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00755">
        <property type="entry name" value="ksgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727:SF7">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 9" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 8 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 8 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA adenine methylase transferase N-terminal" evidence="11">
        <location>
            <begin position="46"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2069725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="317" mass="35760" checksum="EF88A57AD9AB2707" modified="2022-05-25" version="1">MVKPVRIGEKHGSKGDDSKVSKTVTRHHEFQMNKTYGQHLLKNPLIIDSIVEKSQLKSTDTVLEIGPGTGNLTMKLLENCKKVIAVEVDPRMAAELQKRVAASPYANHLEIILGDFLKVDLPYFDVCVANVPYQISSPLTFKLLAHRPIFRSAVLMFQREFALRLAANPGDSLYCRLSVNTQLLSKVTHLMKVGKNNFLPPPKVESAVVRIQPFNPPPPINFVEWDGLVKLCFSRKNKTLSGIFRVSSVIETLNQNYKTFCALEGKMDTDGSDEQMKDKIIKVLEDNHFLEKRSSKLDINEFLKLLNCFHGAGIHFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMQ6</accession>
    <name>A0A8J4PMQ6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_007894</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">PML body</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000446">
        <property type="protein sequence ID" value="KAF2070780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMQ6"/>
    <dbReference type="OrthoDB" id="1305878at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16503">
        <property type="entry name" value="RING-HC_CHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000203">
        <property type="entry name" value="E3 ubiquitin-protein ligase CHFR isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040909">
        <property type="entry name" value="CHFR_Znf-CRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052256">
        <property type="entry name" value="E3_ubiquitin-ligase_CHFR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16079:SF4">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16079">
        <property type="entry name" value="UBIQUITIN LIGASE PROTEIN CHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17979">
        <property type="entry name" value="zf-CRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="77"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="175"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2070780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="433" mass="49538" checksum="8E3126A1EB90EB34" modified="2022-05-25" version="1" fragment="single">ISNSNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNKQDDGDDKNNLSSVIAVDNTTTKKSKVDLEDSMGENLICGICQEIIYKCITLVPCMHNFCTCCYGNWRANSPDCPQCRGVVKLGQKNHAINNLIEIYLQKNPEKKRDPEELADMDKTSKITDEMLIKGILNKSKNKNGYDDYDDEDDEEEYYDEEDSDDEENYNYNHNHNHAQAPLHPPLFGVQTQKCFCCTTPAPNGYQCPAVNPPHLFCTTCLQAFPRETPEPRGVRCDFCSRANCSIYRTAANPAPHVLKFSMLSEFDPLNIPPNAFLGNQYEAKVMMDYTVKSGKTLSQIYKEIIQQIDEQKINLVTPVRRINQMLNGAPMPTYANTYSCDYCMKELFAQALFYYRKAIPKDDLPDSGKRDDCYYGKNCRTQFNKPDHAKRFNHVCDQTKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMQ7</accession>
    <name>A0A8J4PMQ7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009509</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000727">
        <property type="protein sequence ID" value="KAF2069171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMQ7"/>
    <dbReference type="OrthoDB" id="659430at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="100" mass="11467" checksum="6673F4A9371F71C6" modified="2022-05-25" version="1">MVSKPQIKSSSQFPPNLKKLTIPCCSRVLDIVPTSTIHHLQFNNNIIQNPTDTLIFQVELTPPNITKLILNDDNVCFETYNQKHQTLSLDGTRYYNTSYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMW5</accession>
    <name>A0A8J4PMW5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Short-chain dehydrogenase/reductase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_008500</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000530">
        <property type="protein sequence ID" value="KAF2070173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMW5"/>
    <dbReference type="OrthoDB" id="1274115at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004825">
        <property type="entry name" value="PRK06181.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43391">
        <property type="entry name" value="RETINOL DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43391:SF5">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="287" mass="32134" checksum="4FA9138609F563CD" modified="2022-05-25" version="1">MATSLSSSKVIVITGSSSGIGQELALIYSRRPNVNLVLASRNTEKLNALATKILELNPNCKCLVVSYDASKEQDCKFLIETVIKEFGRLDLLLLCAGVSYHNQFKDTKDLNVYREMMDINYFGYMYTTFYALPFMIKQHEKENGVKPQIGVISSVSGELGLPLRAGYCASKFAVNGFFQALRLEVDKHIDITLILPTSVNTPMRENSLGKDEIKNVHFNEDESKRMSINVCCEIITKSIDCRKKKVVFPFSNYLATVLQPVFPTFIENILKKKAGGNATNNHPKAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP32</accession>
    <name>A0A8J4PP32_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_008575</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000542">
        <property type="protein sequence ID" value="KAF2070101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP32"/>
    <dbReference type="OrthoDB" id="18376at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021475">
        <property type="entry name" value="Pants/Emi1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28052">
        <property type="entry name" value="UPF0545 PROTEIN C22ORF39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28052:SF1">
        <property type="entry name" value="UPF0545 PROTEIN C22ORF39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11326">
        <property type="entry name" value="PANTS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2070101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="200" mass="23699" checksum="26012E4933B5AA6B" modified="2022-05-25" version="1">MMNMNDEIESKERELYNKFLNNRNITIIEKEEETIFIPPNDYNDDQVTYQTTESLDLPNSILDHSFSCNSFLKNFISCHSPAGMIRGLYRYGENKCKVEDDNLFDCYQVKFSGSSDGRKKKMTEILDKRIDRDNEFYKNHLWKRRFIPKKSSQVDRKEVDDKDLYKADLQIDVTMFGYTQELYSFLTNPNNSTNITEVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPC6</accession>
    <name>A0A8J4PPC6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GH18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_006921</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the degradation of asparagine-linked glycoproteins. May hydrolyze of N-acetyl-beta-D-glucosamine (1-4)N-acetylglucosamine chitobiose core from the reducing end of the bond.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000341">
        <property type="protein sequence ID" value="KAF2071755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPC6"/>
    <dbReference type="OrthoDB" id="16630at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009313">
        <property type="term" value="P:oligosaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000250">
        <property type="entry name" value="Probable di-N-acetylchitobiase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051887">
        <property type="entry name" value="GH18_Domain-Containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46290">
        <property type="entry name" value="DI-N-ACETYLCHITOBIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46290:SF1">
        <property type="entry name" value="DI-N-ACETYLCHITOBIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0458">Lysosome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="GH18" evidence="9">
        <location>
            <begin position="1"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2071755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="365" mass="41008" checksum="18C343D9092D9F11" modified="2022-05-25" version="1" fragment="single">KDIKSYDIDPWTSSCPCSDSSLCNPVSVGERQEFMGYSVNGGPEYYNNYDYSTLTSIASFYGPVSSDLVCMAHAKNVRIVYAAYYPIVQLGNMTYQQEWIQSKVELVQSTYADGLNFDVEDPIWKNMSEIAALYTSLVAETTYVFKKLNPNYQITVDIGWRDCDDGRCYDYVGLSQASDYMIVMDYDMQSQIITNDCVAGANSPPSWIIQGMHNFTGSLGIPVNKLLMGLPWYGYSYQCIGSTASINNTVCHLKLDPYLGVNCSDAVGSEIDYGKIMNIIANNTIEKSTPQWDSVNYSPFFNFRADDGTIHQMRYDNPASLSKKVQIAKDLKLRGVAVWNIDELDYQNNPTQSSAMWSSLNGFFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPD7</accession>
    <name>A0A8J4PPD7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_007313</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000382">
        <property type="protein sequence ID" value="KAF2071373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPD7"/>
    <dbReference type="OrthoDB" id="951172at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2071373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="762" mass="88838" checksum="1F4274BBA1704B95" modified="2022-05-25" version="1">MGRDKTKIENHFTECKDYRSSNNLRKLNALFNHKELLSIQDRLKVGERIKELVVEGGPKQFEVNPNLLLDTNQEISDFVLGLVDNNFMIDWAIIVEKLLEFGNNRDTINREHYYSIACKLLENIKRDFDNEDLSEFGETLIKSLKHTTNSNYRSLLLTTLSEIIVQDDVPIAEELIKALYNLLDDTNINVIASILLCYKSLLVHYQQEVLEVIPSIKWFLFETMKYPCEKVSLPSTQFWVDLISSTNDLSFISNDLQEFHLTLIENLIYSDYNIHLLNNENNTTRFELYTLRKKTAVLIGVIANIIEKDDVLPQLLPVIEDKLQRQNDWKIRETGIFVLGLLKYDTYFRANLDKLIKTTSECINDDQHMWLQYTSWWTLGNMAKFILQSNNEKLIDTVFNLYIDQISTPIDQLQEALCLSLYPFLVELEKVWMPSTLPYFTLIIEKLLLCYPSYSDDILSLVDDSLTLLVKILHEKKIDTQEISQLYHHHLSVALAGNMGDKFIKAFKSLCLFSTASQYENNHFDSLMEICIKLIKSDLNQEENIQEGLRGLGFLFKSRSFENLENDKLNEILSLLPRLYKVEASKVYTIYKYLIKKVISNFNPLQQLMAKLSNKIGSQLSNPIFSKLMKKIIEKFPDQVDYLFSDEFLGAFDKQGIQQNLLLFNVIAKQYPEKIVLIITPYIEACSSFTGDKTDQQKEKRYQELLEFLKLYIVSKKRKQVMLSSIFSTLGRKFESDSSSLNPTLVQEFKNIIYKLKELYRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPI3</accession>
    <name>A0A8J4PPI3_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007545</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000409">
        <property type="protein sequence ID" value="KAF2071139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPI3"/>
    <dbReference type="OrthoDB" id="20903at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098887">
        <property type="term" value="P:neurotransmitter receptor transport, endosome to postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099152">
        <property type="term" value="P:regulation of neurotransmitter receptor transport, endosome to postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026204">
        <property type="entry name" value="GRIPAP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18978">
        <property type="entry name" value="GRIP-1 ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18978:SF1">
        <property type="entry name" value="GRIP1-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="674"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="96"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="243"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="423"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="580"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="674"/>
            <end position="699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2071139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="920" mass="105729" checksum="E03B1A76532DDD58" modified="2022-05-25" version="1">MAGFSDEDFNLLQEQLIDLKRYKYESSEREKKYQSEIKSLKDQVEEVSHNNNNGKNKTSFFSDIVNKAKDKGKLNEENEILKKTIEENTIVHKEQNEALKHNIKSLFETNSQLEEQILLLKKDLLQNVVKSEDQQNIIRKLQIEVAELHCSKEELENKVVTNSNNSLNSSSESNNTTNSHNSNISLESILSIIEQPEQNSDNLKSKIIELFTNSVPTTPPLPTPSLSPKEAIFHPLGEPEINVVAVDQEKQKLLDTLKNLMEQVRDNDSITNKLQNEIKQHLSTIEQLNIQSNQFQEKHRSAEINRVKIEERYDQEIQKLNQEIQNLQNEKEQSSKLIQNIETNTLTTSKNFHESISLLEESLEKEKKKLEEKENEIKDIKLDFERQLSDAQDKSNIFKLSRDEYLEKYNQVTQEVLLRDENLQLKSQEMANLSKQISNLEIQLEESKRLLLSSQTDIENNISQQESQTTQIKNLSDKLAMERKSFDSLTEQFNVLTLNFSNLEKTNQALEKEKLESSKEIESLNSSILNLESNLNQLNEKYEKVDKELASKIEIEKEQSSKIESFENQLQGLVDLPDQILEKNQVIEQMEMEIKEIKEKLVAIEPEFEKTKADYKETCEKIVELQVALQDAETEKKIVEKKNAKMIKDLKVELSRERDSVSQLSQSANNVLTTMTGSPSMPNLSQSSHSLPTTPSATPVPSRHTRSISRDFNLNSPMKNNNIPMYPNQVVVNNNSNNNNELLKQDLEILGRKLGQLGTERYKLEERVRLLESENATLVSELEKKTKLTRFYISRTQLGRATSEDEKTKRMKGGASGSFWRSNDPKIVGEIVEKMEIMLQENILKNMQLTDDISLFGSQVSKLENENTYLKSLLSKNGIDQPTFESLHQLMSAENIVVTPATPKSIEITNDNNNSNESLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPM7</accession>
    <name>A0A8J4PPM7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007523</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000406">
        <property type="protein sequence ID" value="KAF2071168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPM7"/>
    <dbReference type="OrthoDB" id="23586at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="163" mass="17665" checksum="9AAF32A46579375B" modified="2022-05-25" version="1">MVGDFLTSFEKVNKAVTASFGKLANRIGPILSKIAPAKLGSFFSKYFTKIFTSNANNFLTYRVCPLLLLVAVYNNIWDAKASLEEKNWGGFACDLAQIGCNIIAAGILLAGIPFAAVGALVLGALAVGFFLIKMIFFPAKSPIQKYFESQYFPKKYIKTAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQT2</accession>
    <name>A0A8J4PQT2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RNA-splicing ligase RtcB homolog</fullName>
            <ecNumber evidence="10">6.5.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">3'-phosphate/5'-hydroxy nucleic acid ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_006283</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP + H2O = a ribonucleotidyl-ribonucleotide-RNA + GMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68080"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP = a ribonucleotidyl-ribonucleotide-RNA + GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:68076"/>
            <dbReference type="Rhea" id="RHEA-COMP:10463"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83062"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="10 13">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Catalytic component of the tRNA-splicing ligase complex.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="10">Ligation probably proceeds through 3 nucleotidyl transfer steps, with 2',3'-cyclic phosphate termini being hydrolyzed to 3'-P termini in a step that precedes 3'-P activation with GMP. In the first nucleotidyl transfer step, RTCB reacts with GTP to form a covalent RTCB-histidine-GMP intermediate with release of PPi; in the second step, the GMP moiety is transferred to the RNA 3'-P; in the third step, the 5'-OH from the opposite RNA strand attacks the activated 3'-P to form a 3',5'-phosphodiester bond and release GMP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the RtcB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.8" evidence="10"/>
    <dbReference type="EMBL" id="AJWJ01000283">
        <property type="protein sequence ID" value="KAF2072408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQT2"/>
    <dbReference type="OrthoDB" id="10249697at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072669">
        <property type="term" value="C:tRNA-splicing ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003972">
        <property type="term" value="F:RNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0170057">
        <property type="term" value="F:RNA ligase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1860.10:FF:000001">
        <property type="entry name" value="tRNA-splicing ligase RtcB homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1860.10">
        <property type="entry name" value="tRNA-splicing ligase RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03144">
        <property type="entry name" value="RtcB_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001233">
        <property type="entry name" value="RtcB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036025">
        <property type="entry name" value="RtcB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027513">
        <property type="entry name" value="RtcB_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118">
        <property type="entry name" value="RNA-SPLICING LIGASE RTCB HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118:SF1">
        <property type="entry name" value="RNA-SPLICING LIGASE RTCB HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01139">
        <property type="entry name" value="RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103365">
        <property type="entry name" value="Hypothetical protein PH1602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01288">
        <property type="entry name" value="UPF0027"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 10 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0819">tRNA processing</keyword>
    <feature type="active site" description="GMP-histidine intermediate" evidence="10 11">
        <location>
            <position position="428"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 12">
        <location>
            <begin position="226"/>
            <end position="230"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 12">
        <location>
            <begin position="353"/>
            <end position="354"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 12">
        <location>
            <begin position="402"/>
            <end position="405"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 12">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 12">
        <location>
            <begin position="428"/>
            <end position="431"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 12">
        <location>
            <position position="504"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2072408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="505" mass="55057" checksum="2A241BD72901EDE9" modified="2022-05-25" version="1">MSRSYNEEIGFIEKIDSVRYKIKKGFVPNMNVDGLIYVNDSIKDLVFEELEQYSTRGSAGGFLPAVKQIANVASLPGIVGHSLGMPDIHSGYGFSIGNVAAFDTEDPMSVVSPGGVGFDINCGVRLLRTNLTEKDINPIKEQLCQSLFDHIPVGVGSKGVIPMNAKSLDAALELGIDWSLGEGYAWVEDKEHCEEYGRMLNADPTKVSDRAKKRGLPQLGTLGAGNHYLEVQVVDEIFNKTAASKMGIDQVGQVCVMIHSGSRGMGHQVATDSLTLMEKAMARDHIHLNDKQLACSRINSQEGQDYLSGMAAAANFAWVNRQSMTFLARQAFAKAFNSAPDDLDMHVIYDVSHNIAKFEEHMVDGRPKKLLVHRKGATRAFPPHHPLIPVDYQFTGQPVLIGGTMGTCSYVLTGTELGMKETFGSTCHGAGRSISRNKSRNNLSYEGVLDSLQSKGISIRIASPKLIMEEAPESYKDVVQVIETCHKAGISQKTVKLRPIAVIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQY9</accession>
    <name>A0A8J4PQY9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RNA polymerase II elongation factor ELL N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000383">
        <property type="protein sequence ID" value="KAF2071361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQY9"/>
    <dbReference type="OrthoDB" id="10683222at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="390"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="437"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="620"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="238"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="452"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="479"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="538"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="620"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="657"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="673"/>
            <end position="699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2071361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="819" mass="90065" checksum="5D3092464DEAF9A5" modified="2022-05-25" version="1">MDEGVLKTNKEPKFVMSLKLSADLVDKIENNKNKVQFFYGEESKILIDGVEYIFSTPMDTKLQVCLLQENNQYTEIGSVMKKLNLESSLTSDDRKRIKSSAKEAELNRKRHKTVLLGGGKTTTTTSTSSTNMLAKSNIRKPKVLNLPKAVPPPPIPIITHTNVVSSSTSSSSTSSTVSSSTTNPLSPQTNTNISIPASPTLSPDLPPHTSSSSTSSSPNISNGMARVVVSPLPISPRSTTSSSSSSSSSNNSNNFDKHIDSSTTTTTNTLTPSTTTTSTKQVKKKHTPAVYLGLKGEQMEKHLLHSLPFGLTPQLLKIRYEIDVANPFLNKIAVFKSPGVWILKDDYFVHINIDWSEYKSDEREIIKTKRKEALTRLNLPIDTPFIDYSTLNNDKSNSNNNNNSSSNNNNNNNEKEKENSLANEIEKIVPGVKNIESKTSSSIKKPSKKESSSSTLSSTSSSSSTKPLTPTLNIPPIPLLNTNNRSASPNLSSPNVSPNHIPNNNNNNSSSNNSNNKTIKDSDLLPWSKPKPQKPEASTNNNNNNNNNNNNYTSSNSSSINSNSNNTAKLAPIPSSYPSTIITPPPLPSKNAFKNKTNFLKNVDQKINDLHQNITPIVSAASTSSSSTTSIPSSSSSSSSSSSSSSTINGNTNTNGIRKETKKENGSIKEKLSTTNNNSSSNSTTTTTTTTTTNNNNNNIVNQIYKDTYKEPMVISHIKNEDDYKIVLEMSQNKYNRYTQIHKFICENIQDFKGLQKNLESCTNEEQRKKLTENIQSLYVERKEKVRVEKEEYQRLHQELISLKKILKDFNINKSSSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR25</accession>
    <name>A0A8J4PR25_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">CYY_006081</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000265">
        <property type="protein sequence ID" value="KAF2072603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR25"/>
    <dbReference type="OrthoDB" id="10261027at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09487">
        <property type="entry name" value="SAM_superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001060">
        <property type="entry name" value="Probable serine/threonine-protein kinase DDB_G0278535"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF288">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="75"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="145"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="180"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="14">
        <location>
            <begin position="235"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="357"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="297"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="303"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAF2072603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="644" mass="73316" checksum="8229EC8EAF0697AD" modified="2022-05-25" version="1">MASNNELVLAVQQNDLAKVKKLASKKKAKIQLVTFDQFGQSPLSIALKNNQEDMVDILLGSYTQTKADINVRDKNGYAALHQAVSSDDKILMKVLQHEGINVDVQNDDKNTPIHYFCQTFRSPNCQEPFQLFIQKGVNVNAQNKNGETPLHKAIFNNSVRLMMVDLLLKNGANVNLVNTNQESPLHYAVRLGREDLVSVLLKAGADVNCVGTKERKSPYDLALEMNNKEMSARIKKYKDLFAWLQENSLEQYKDAFVKEEMFKDELGGMNEKILDKMGIQAAGHRLRIIKATSILREQRDQDSNSNNSNANSNHIDARDEEDSSPPDTPDISGLKDSLHALKHIKMHDVNFILDSELEYTEKLGAGSSGKVYKGLYKNKEVAIKVLKSMTEAKEIDEFKKEFQIMSAIRSKHVVLFYGAVLEPKLCMVMENCGRGSLYHVMKDESLDIRWEKTFRFALETVKGIECLHNWEPSIVHRDLKSLNLLVNDKWEVKVCDFGLSRFNTGSNLETLVKMRGTFAYCAPEVYFGEQFSSKSDVYSIAMILWELVTRCINGRYERPFSEFKNLHHDFQIIIQTAKKGLRPSIPKACPQSLALLIQDCWDFNLEKRPSCGEILKRLLEIETEYKSNVETWEQCIVPLGPNVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PR91</accession>
    <name>A0A8J4PR91_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005914</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000249">
        <property type="protein sequence ID" value="KAF2072768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PR91"/>
    <dbReference type="OrthoDB" id="41532at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050769">
        <property type="entry name" value="NAT_camello-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13947">
        <property type="entry name" value="GNAT FAMILY N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13947:SF37">
        <property type="entry name" value="LD18367P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="78"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="181" mass="20136" checksum="CFA9117A42D56B64" modified="2022-05-25" version="1">MNNSFKIERFEGIPSQLDLSNLSSLLYDCVVDGASIGFLTPFSVEKARVFWDGLVNQFQSKSRILFVARDISKSSSSGNIVGTVQLILDMPDNGVHRGEIVKLMVSPLYRRMGIAKQLLEIAEQVAKEYKKTLLVLDTNTGEGAELLYQSIGYKLCGVIPNFSISSVDRSLKPTSYMYKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRB6</accession>
    <name>A0A8J4PRB6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_007190</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000369">
        <property type="protein sequence ID" value="KAF2071495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRB6"/>
    <dbReference type="OrthoDB" id="18672at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="204" mass="23645" checksum="D2087F4484E8791F" modified="2022-05-25" version="1">MQYLDSLENSEDIKNTIFKVFENERTLYNYSRENLKTMAVTIHPHFTVWLASLQLMKVGGKAMKHNAFEEVFGTNVFNEGPHSQLALKTFRQLGPYTIENYERCQDAIKNIYRNVNPEKINVLYAFLICCELASFLFIDFKDALIKLGVQDLFYFDVHLQADNLIDGHGIQMLKALKTDTNMTISQFNQGIQLFVDFFGSIFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRQ3</accession>
    <name>A0A8J4PRQ3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nuclear condensin complex subunit 3 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_005511</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CND3 (condensin subunit 3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000222">
        <property type="protein sequence ID" value="KAF2073158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRQ3"/>
    <dbReference type="OrthoDB" id="27187at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000793">
        <property type="term" value="C:condensed chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027165">
        <property type="entry name" value="CND3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025977">
        <property type="entry name" value="Cnd3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14418">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14418:SF9">
        <property type="entry name" value="NUCLEAR CONDENSIN COMPLEX SUBUNIT 3 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12719">
        <property type="entry name" value="Cnd3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nuclear condensin complex subunit 3 C-terminal" evidence="3">
        <location>
            <begin position="628"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="162"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="972"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1057"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1156"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="26"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="973"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1002"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1157"/>
            <end position="1180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2073158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1237" mass="141716" checksum="64ACCD7452C48BE6" modified="2022-05-25" version="1">MSVNSKKQITTKTKQQQQQKKKDSNKSSSSNSTNSNSVNITTKNSNSSSSSTSNTLVSHITEQFKYSSNIKPLRSSKNIGISTSSSNNNNNNNNNDFKIYSSIFFQLDKLLKKCRDTNLFFEAFLSPLKIFLVLDNNKSAINITHFLSKYLYYLNSDSIDNSSNNGNSTTSSDDEEQDNKKNDKQNKQLLINLKNQRQKRQQLYEYIVDFLITVLESKDSTVRLRSLQIFNTMINDDIHFNLDKYMNKLLFNNIVNRCQDRISIIRQVGGNLLIKLLDLYSRLKDVNEIVKASKDILVDELLVMLENDPNWMVRNSILSQLIEFKQLFVSSQDELIQLILKRTIDIKEEVRVQSYRLLSSNFKFDQFSKEQILFIISNGIKEDPSEKIKAACGDLLCLWFQDMNVKHWIYRFLESVNVVENYQLLETVLLYLFEKTEIPEFNLKESAHRELTIEEAFHCKLTCKYMKSLKSLGEERLEQLLPTLTQYRDILFNHLSADPDTFSFLLDVLNCFDMADEVGRSNLIVFLRELLKTVNQEQLNLLDEILVSLSLLHHSEKDFIVFVVEIIQDMMDPLEDDPNEERLQVLKKTFERLDGKIASEAKKIKDTIDQIERSLVEKQTFTLLKSSIITHSLLLNAKKCSNSFELDSLVQSVILPSIQHVDPEIREFGIKNLGIFCLHKRDTALTYLNLFEKVVENDILKVQQTCITVIFDILLVFGSPRKIPKETKSLYKVIRKYAKEVQNGQIKEICIQGFCKLLYAGIVYDPKILSFILLEYFATKQENPSIHRCLDLFFQAYAVDMIENKKLLFDISMDVFQTITSSPSQSPYFSINLIEALKYILHLIEKPINSKSSSISTADFSIQDCQPLLALSICQHLIKCFRGPTADLSKLLPVLKIDREKHSGLIERIYDALESLFAFHFDNNLDKFNTTIKKLVSKEYLESKLLSQSQHSQSQQTCLDPDTLLETIKAMESNSGSGNNGASGNPNNSQKKKKVNGSKIFDTPKKLSEKESSTKYMPLNNEILEISNSLSRASKSSSLSKFKSYFQNQRNYFDSEAVIDKSPKKPKRKLTTTTTKPNDNDDDDNYNHIDPFQDEQEEEADDQRFNAFILSKKNIPTINSSKSILKSTTSTANGNNNNINITSKINNQLDIQKHTKNSTSTTTTDNNNNNTINDGNINNNSKSILKKRKSIDPKIATTNNTTATTKTTTTTVGGGADDQNNNFRKKVRFNSFVEEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRT3</accession>
    <name>A0A8J4PRT3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Aspartate aminotransferase</fullName>
            <ecNumber evidence="8">2.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_006871</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21824"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="EC" id="2.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="8"/>
    <dbReference type="EMBL" id="AJWJ01000336">
        <property type="protein sequence ID" value="KAF2071810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRT3"/>
    <dbReference type="OrthoDB" id="6752799at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000015">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000001">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006719">
        <property type="entry name" value="PRK09257.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF22">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="9">
        <location>
            <begin position="49"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2071810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="422" mass="46745" checksum="13D3E57F2A599871" modified="2022-05-25" version="1">MISRQIIRNLSKLNSTRLYSTSWWSNVQKGPEDPILGVSVAFNKDTSPSKINLGVGAYRDENGKPYVLESVRKADKKIFEANVDHEYAPIAGVATFNQLSAVLAFGEDSQHIKEKRIVTVQAISGTGALRIAAQFLARFMPGKTAYVPNPTWGNHNVIFADSGVAVKSYSYYNPSTCGLNFDGMFNDIKAAPNGSIILLHACAHNPTGVDPTAEQWKKISQLCKEKEHFVLFDFAYQGFASGSPEQDAQAVRQFVADGHNIALCQSFAKNFGLYGERIGAFSLLTATADEAVNVESQLKILIRPMYSNPPVYGARLVSTILKDKELTSQWRDEVKGMADRIINMRESLVKYLKKHGSTRDWSHITTQIGMFCYTGLTPEQVDRLASEFHIYLTRNGRISIAGINSSNVEYLAKAMHAVTKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRX1</accession>
    <name>A0A8J4PRX1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_005376</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000212">
        <property type="protein sequence ID" value="KAF2073323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1931567at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097157">
        <property type="term" value="F:pre-mRNA intronic binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030623">
        <property type="term" value="F:U5 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000393">
        <property type="term" value="P:spliceosomal conformational changes to generate catalytic conformation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08056">
        <property type="entry name" value="MPN_PRP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13838">
        <property type="entry name" value="RNase_H_like_Prp8_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.40:FF:000001">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.230:FF:000001">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.43.40:FF:000001">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000002">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1570.40:FF:000001">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1570.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.40">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012591">
        <property type="entry name" value="PRO8NT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012592">
        <property type="entry name" value="PROCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012984">
        <property type="entry name" value="PROCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027652">
        <property type="entry name" value="PRP8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021983">
        <property type="entry name" value="PRP8_domainIV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043173">
        <property type="entry name" value="Prp8_domainIV_fingers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043172">
        <property type="entry name" value="Prp8_domainIV_palm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019581">
        <property type="entry name" value="Prp8_U5-snRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042516">
        <property type="entry name" value="Prp8_U5-snRNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019580">
        <property type="entry name" value="Prp8_U6-snRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019582">
        <property type="entry name" value="RRM_spliceosomal_PrP8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11140">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR PRP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11140:SF0">
        <property type="entry name" value="PRE-MRNA-PROCESSING-SPLICING FACTOR 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08082">
        <property type="entry name" value="PRO8NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08083">
        <property type="entry name" value="PROCN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08084">
        <property type="entry name" value="PROCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12134">
        <property type="entry name" value="PRP8_domainIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10598">
        <property type="entry name" value="RRM_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10597">
        <property type="entry name" value="U5_2-snRNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10596">
        <property type="entry name" value="U6-snRNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="MPN" evidence="8">
        <location>
            <begin position="2091"/>
            <end position="2221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="2302"/>
            <end position="2323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="2310"/>
            <end position="2323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2073323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="2323" mass="271788" checksum="C2F52AE4BEDE823D" modified="2022-05-25" version="1">MEEDSKYHMMDTSIEAKDKAQKWIQLNNKRYSEKRKFGYSEPQKEDMPPEHLRKIIKDHGDMSNKKFRHDKRVYLGALKYMPHAILKLLENMPMPWEQVKNVKVLYHVTGAITFVNEIPFVIEPVYIAQWGSMWVTMKREKRDRRNFKRIKFPLFDDEEPPLDYSDNILDVEVEESIQMELDEEDDAAIIDWFYDSKPLSRTKFVNGPSYKKWKLDLPIMSALYRIASPLLSDLVDKNYFYLFDDNSFMTSKALNMAIPGGPKFEPLFRDVDDDDEDWNEFNDINKLIIRNRIRTEYKIAFPYLYNNRPRKVHTPFYHAPNNCFIKTNDPEIPGFYFNPQLNPIPSYKTTKTAPANNYGDEDDDFTLPDNVSGALLPDVDYENPLVNDAVNLYWAPRPFNLRSGRTRRAEDIPLVKTWYKEHCPPEHPVKVRVSYQKLLKCHVLNKLHHRPPKSMNKKNLFRALKATKFFQSTEIDWVEAGLQICRQGYNMLNLLIHRKNLNYLHLDYNFILKPIKTLTTKERKKSRFGNAFHLCREILRLTKLVVDIHVKFRLGACEAFQLADGVQYLFSHLGLLTGMYKYKYRLMRQIRMCKDLKHVIYYRFNTGAVGKGPGCGFWAPMWRVWLFFLRGIVPLLERWLGNLLARQFEGRQTKGMAKTVTKQRIESRFDYELRAAVIHDILDMMPEGVKANKTRVILQHLSESWRCWKSNIPWKVPGLPIPIENLIIRFVKSKADWWTNVAHYNRERIKRGATVDKTLCKKNLGRLTRLWLKSEQERQHNYLKDGPYVSAEEAVAIYTTTVHWLEKRRFSAIPFPQTSYKHDIKILTLALERLKEAYSVKSRLNQSQREELALVEQAYDNPHDALARIKRHLLTQRTFKEVGIEFMDLYTHLVPVFDVDPLEKITDAYLDQYLWYEADKRQLFPNWIKPSDNEPPPVLIHKWCQGINNLDRIWETSDGECVVLLETQFSKVYEKMDLTLLNRLLRLIVDQNIADYMSAKNNVVIHYKDMSHTNSYGLIRGLQFASFIFQFYGLVLDLLVMGLNRASEIAGPPNLPNAFLTYPNLNTETKHPIRLYSRYVDKLYILYKFNNDESRELIQKYMSEHPDPNNENVVGYNNKKCWPRDCRMRLMKHDVNLGRAVFWNVKNRLPRSLTTIEWEESFVSVYSKDNPNLLMGMCGFDIRILPKCRTNADQLVPKDAVWSLQNVNTKERTAQAFLRVDKDSQDRFENRIRMILMASGSTTFTKIVNKWNTALIGLMTYYREAVVTTREILDLLVRCENKIQTRVKIGLNSKMPNRFPPVVFYTPKELGGLGMLSMGHVLIPQSELRYSKQTDTGITHFTSGMSHDEDQLIPNLYRYIQPWEQEIKDSQRVWAEYAIKYEEAKSQNKNLTLEDLEDSWDRGIPRINTLFQKSRHTLAYDKGWRVRTDWKQYQVLKSNPFWWTNQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKFKKLTHAQRSGLNQIPNRRFTLWWSPTINRKNVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESLVMDLCQVFDQELDNLEIGGVNKEAIHPRKSYKMNSSCADILLRATHKWQVSRPSLLTDNRDSFEGSTTQYWLDVQLKWGDFDSHDIERYSRAKFLDYTTDSMSFYPSPTGCLIGLDLAYNIYSAFGNWFLGVKPLVQKAMAKILKSNPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNKIVWFIDDSNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTDVWLGQKRLAQLAKWKTAEEVAALIRSLPVEEQPKQIVATRKGMMDPLEVHLLDFPNIVIQGSELQLPFQACLKIEKFGDLILKATEPKMVLFNIYDDWLATTPSYTAFSRLILILRALHVNLERTKIILKPDKNTITQPHHIWPTLTPEEWVKVEVELKNLILADFGKRNNVNVASLTQNEIRDIILGMEISAPSQQREDQIAEIEKQKKEASHLTSVTVRSTNIHGEEIISTATSPHETKVFSSKTDWRVRAISATNLHLRTNQIYVNSDLAKESGFTYVFPKNILKKFITIADLRTQIMGYIYGVSPPDNPQVKEIRCIVMVPQWGTPVFVNVPNQLPEHEYLKELEPLGWIHTQPTELPQLSPQDVITHSKIMSDNKQWDGEKAVVISVSVSWPCTLTAYRLTPSGFEWGKANKDSLNYQGYQPTFYEKVQMLLSDRFLGFYMTPDRGSWNYNFMGVKHSPNMTYGLKLDYPKSFYDEAHRPSHFQNWTQISDTTNDENEADNENLFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSC6</accession>
    <name>A0A8J4PSC6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SWAP/Surp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_005765</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000239">
        <property type="protein sequence ID" value="KAF2072908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSC6"/>
    <dbReference type="OrthoDB" id="2017782at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.790">
        <property type="entry name" value="Surp module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051485">
        <property type="entry name" value="SR-CTD_assoc_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000061">
        <property type="entry name" value="Surp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035967">
        <property type="entry name" value="SWAP/Surp_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23140:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23140">
        <property type="entry name" value="RNA PROCESSING PROTEIN LD23810P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01805">
        <property type="entry name" value="Surp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00648">
        <property type="entry name" value="SWAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109905">
        <property type="entry name" value="Surp module (SWAP domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50128">
        <property type="entry name" value="SURP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="126"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="232"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="SURP motif" evidence="5">
        <location>
            <begin position="400"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="CID" evidence="6">
        <location>
            <begin position="478"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="74"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="197"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="304"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="74"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="200"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="304"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="344"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="361"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2072908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="620" mass="71207" checksum="A955160314EAB621" modified="2022-05-25" version="1">MNTFSNDGSFMEQFLKRQKVSSGDVADASAVNEGYQQQQQQQYGTEYTGYDQSGYYQQPQQQEGDLQSYYASYYQQQQQQSTSPQQQQQGGYNNNYSNQYQQQQGGYNNNNNNNMNNSMNKMDPTRVVWVGNVHGDNIEDDIRPMFSQFGYIEAIRVVASKFCAFVTFSDINAAIQAQYQLNGTLIRGNPLKLGFGNPESSDRLLQHHGGGGDREGRFQRGGFKSHDEQPSKNLWLGNISPQVTQEMLRSMFEPFGKIDYIRVLTQNRCAFVNYFDVNSATYARSNLNGSPLCGMPLKINFRKEEGRRGGDDGDRDRRGGPPMDRDRGDRGDRGDRRGGDGFYQQQQQPQQRNPNDNNSSDSRRPPNEGGGEKPAFRRPFRENQAPMPTPPPRDPNEQIVVDKFAEYVARVGPRMETLALDKQKNNPLFKFLNQGNQSHEYYRWKLWTIKNPDAHSATNDPEQQQQQQDQLQYPTKFLTPQEVEEFRNLLERLTPTKLSISGCKNWIMSHLLNSLEIISIITSTFQKVNLPFERRLNILHVLNDCLHNTINYKKPNPDDPDIDFAQTIKGYLEFIVGSTSYGESNESQQKVLKVLNIWEQKQIYPPDFIAKLKEIAIDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSF9</accession>
    <name>A0A8J4PSF9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glutamine synthetase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_007037</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the glutamine synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000354">
        <property type="protein sequence ID" value="KAF2071644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSF9"/>
    <dbReference type="OrthoDB" id="77835at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006576">
        <property type="term" value="P:biogenic amine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000005">
        <property type="entry name" value="Probable glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43785">
        <property type="entry name" value="GAMMA-GLUTAMYLPUTRESCINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43785:SF12">
        <property type="entry name" value="TYPE-1 GLUTAMINE SYNTHETASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51986">
        <property type="entry name" value="GS_BETA_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS beta-grasp" evidence="7">
        <location>
            <begin position="88"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="GS catalytic" evidence="8">
        <location>
            <begin position="195"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2071644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="532" mass="59976" checksum="E969353AEDDCCEDF" modified="2022-05-25" version="1">MINLFQKTHKLNSSTFLKNGLKSYCTSSSNNNGNKTIKLDFIEPNKYREKINNFSSTSPKPASTSGNSLNSADDSVYSVLNTEDLILNQIKISKSPFIKVAGADIDGVLRGKYLDKDKFESAVKKGFGFCSVIFGWDSGDVCYDNVAFTGNHTGYPDVNAKLDVGTYRTIPWEYDVPFFLMDFVKPNGEPLPICPRQVLKRVVRMCQDHQLEPLMGMEFEWYNYREDNHTIREKSYNNLTPLTNGMFGYSLLRTSQNSEFMNSLSELRGFGVPLEGLHTETGPGVYEAAIRFSSALEAGDRAILFKQSAKEIASLQGIIASFMAKPYQHLPGCSGHMHQNFASPDGKNLFHDPSAPNEMSEMFKSFVAGQLLLLPEFLPFFAPNINSYKRLVDGYWAPTTPTWGIDNRTVAIRVLKQGKATRSEFRVPGSDVNPYISIAASFAAGLYGVLNKLELKSPPVSQNSYDLYKQGLVSRLPRSLSESTELLAKSTIAKELLGAEFVDHFVETRRWEYRQFNHQVHKWELERYFEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSY3</accession>
    <name>A0A8J4PSY3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit F</fullName>
            <shortName evidence="4">eIF3f</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_005764</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eIF-3 subunit F family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000239">
        <property type="protein sequence ID" value="KAF2072907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSY3"/>
    <dbReference type="OrthoDB" id="25498at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08064">
        <property type="entry name" value="MPN_eIF3f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03005">
        <property type="entry name" value="eIF3f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027531">
        <property type="entry name" value="eIF3f"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024969">
        <property type="entry name" value="EIF3F/CSN6-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540:SF6">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13012">
        <property type="entry name" value="MitMem_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MPN" evidence="5">
        <location>
            <begin position="15"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2072907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="285" mass="32035" checksum="5B12E19E7FB6E344" modified="2022-05-25" version="1">MQKESSIFLPSQVSVKVHPVVIFNILDHYIRRNDNQDRVIGTLLGFNNDGVIEIRNCFPVVHSESEQIAVEMEYQRKMLDLHLKSSPREPIIGWYATGNDINENSVHINNYYREEMGNITPIHLTVDTGLFNDTMGIHAYQAHNLSLTPDSSLGSYFSKLPLEILSFEAENAGLESIAQTKYSENQQPTSLLSELESLKTSLTKLDDMLDQITSYIDAVEKGTIQGDPHLGRFLAKTIQALPKANAQVMDKVINNSVKDLLMIVYLSSLTRSQLAVAEKVRQSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT37</accession>
    <name>A0A8J4PT37_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal protein L22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_005699</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000234">
        <property type="protein sequence ID" value="KAF2072987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT37"/>
    <dbReference type="OrthoDB" id="416470at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="entry name" value="Ribosomal protein L22/L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_uL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047867">
        <property type="entry name" value="Ribosomal_uL22_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_uL22_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13501">
        <property type="entry name" value="CHLOROPLAST 50S RIBOSOMAL PROTEIN L22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13501:SF10">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL22M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="Ribosomal protein L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2072987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="238" mass="26548" checksum="4AB0EF48C22DCDAD" modified="2022-05-25" version="1">MMNQLTKSLRVILNVSSKSANTLSTPSYIYRSAVALNSNTSSSLSSITGHKQLYNYYSTAKPVTGTTSSTSNIFSQSFSAEQPAAAEQQQSGSTKPKTVILSLKNIQYSQWKLQALFKALRGLSYQEAIAQLTFCKKGPAKQIRGLVTQARYVAEYQRDMNPDRLVLNQIWLGRSFFTPSVMVAGRGHMTVLRKPFCHVTVELIEAPVVEGEKKLGKFGKTHKTHAKYNGTFQYNKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT44</accession>
    <name>A0A8J4PT44_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Leucine-rich repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000246">
        <property type="protein sequence ID" value="KAF2072801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT44"/>
    <dbReference type="OrthoDB" id="333024at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052394">
        <property type="entry name" value="LRR-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="788" mass="86945" checksum="D25ED48570E8386D" modified="2022-05-25" version="1">MGISNTDSLTYSTTTTTSSNASMIASTEDQQPLQQKQLFNHDNNEQQPTSDDLALVSEIKQFGTLLIRKYQKHIKVNNQQSFITNIELKETLSKGIVLNNSINLLNSPYKILLQFSLLNFHSFINDYTLKNICNEFQNHIGLLDLSKCKHFTIEALVEIIPSLSNLQFLRLRFCKQITPDNLFTLLKCKSDSLSTLDLSYNCFDSVLFNQRKDNNSLNSSTLLSPPTTTTATDINNNIILNPNIKSLILTHCHLQDEKGELVIKALIQHNNHLKHLNLSGNELSSKTLSLLGNFIKQDTCRLVDIDLGTNMIIDDTSFMEFADSLAHNNSLESLNLESNQIKDQGGAKLAETLKCNKTLTCLNLGSNDLGPDTGVAFGNALETNNSLIKLLLDKTLIDEDGGVSIGHGLESNKSLQYLDLGGNQLENESGVAIANSLKTNNSLTTLVLKRNQFGEYSGKTFGEALKSNTSLRVLDLSGNQIGYKGAKSMAYSLPLNKTLSDLDLSYNLLGDEGGKLICESLGANSSLLRLNLSANRIAVESMKALSISLKNSQSTNYSQKDNQFYNTNNHFYQPSLNLNSNIKDLNLNDNINSNNIDNDNNNNNISLTHTNHNSLYLRNKKTNLLTVSSPNLQHFKTGESTLSNLKSLKLDCNRIGDEGALYIASIISNNPSIQYISVASNHIGEIGGQAIGEALKVNTYLQSIHLDSNSIGTKGAQTISEAIKRNSTLRFLGVCSNRLQDKGIKAIKDSLDHNTTLISISMNNNNVQDDQLLLFLDSLPQFSVDTFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTP3</accession>
    <name>A0A8J4PTP3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CTP synthase</fullName>
            <ecNumber evidence="9">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_005509</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>UTP + L-glutamine + ATP + H2O = CTP + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the CTP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="9"/>
    <dbReference type="EMBL" id="AJWJ01000221">
        <property type="protein sequence ID" value="KAF2073191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTP3"/>
    <dbReference type="OrthoDB" id="1739076at2759"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097268">
        <property type="term" value="C:cytoophidium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03113">
        <property type="entry name" value="CTPS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000207">
        <property type="entry name" value="CTP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000005">
        <property type="entry name" value="CTP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003792">
        <property type="entry name" value="PRK05380.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00337">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="CTP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550:SF0">
        <property type="entry name" value="CTP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTP synthase N-terminal" evidence="11">
        <location>
            <begin position="2"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase" evidence="10">
        <location>
            <begin position="325"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU810713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2073191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="592" mass="65864" checksum="4A2A985FB14A6BCD" modified="2022-05-25" version="1">MKYIVVTGGVLSGIGKGIIASSTAMIIKSLGLRTTSIKIDPYLNIDAGTMSPFEHGEVFVLNDGGEVDLDLGNYERFLGNVSLGRDNNITTGKIYNLVIQKEREGKYLGKTVQVVPHISEEIQLWIDRVAHTPVDGLEGPPDVCVIELGGTVGDIESMPFIEALRQFQFRVGPENFCLMHVSLVPVLGSVGEQKTKPSQQSIKELRALGLSPDFIMCRSSQPLTVETKKKISLFCHVAPENVIGVHDVSNIYRVPLLLNEHNVPALLAKRLQFKLKVDLPTTPTGSDLVPYWLESWKNMADRMDKVTNDSLNPIRIAMVGKYTGLTDSYLSVIKALEHASMEIDRRMVIDWIEASNLEPSTQNKKDYESAWEMLRGAHGILIPGGFGSRGIEGMVLAANYARTNGKPFLGICLGMQVAVMEFARNVMGWSNANSEEFEDAVESEAGKRVVIFMPEVSKTHMGATMRLGARDTIFNNTTAKISQLYNIKTVGQAVEERHRHRYEVNPDVVDEIEKAGLQFVGKDTTGIRMEVVELKDHDYYVACQFHPEFKSRPQKPSPPFVGLILACWQQELQKNLGSHKLIPVLKKWNLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTT2</accession>
    <name>A0A8J4PTT2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Carbohydrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005692</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000234">
        <property type="protein sequence ID" value="KAF2072980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTT2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035327882" description="Carbohydrate binding domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2072980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="189" mass="21233" checksum="B37654D486CCBE51" modified="2022-05-25" version="1">MKYFVVLLLITLAAVNICEATANAFLYKRNENGSYVLQSKAFDPLESGNSFTHTFANANISSYQDFSIEFSDSSNYGKAITIAFDAVDGKGKNINQNFKLKYDGEKIPFPAGVRISVCIPYESTQFFGIALDEDIRTQTIVMSIEKADRTCSYLPVPRYSYKIYNSSSIIKPTFSIIVFIAIVMFTFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUF1</accession>
    <name>A0A8J4PUF1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GH16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004880</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000181">
        <property type="protein sequence ID" value="KAF2073822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUF1"/>
    <dbReference type="OrthoDB" id="20596at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035304782" description="GH16 domain-containing protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="670" mass="74730" checksum="5E44099BC60BA7F5" modified="2022-05-25" version="1">MGRYKSKIIYLLLILLLSSSTVFSQSSSSEEEDSVSYDSEGESESGSDSHQVVVPCATSFSDPLTDTPTNFKQLLTTSTNNPYPQCSLNSNNVNFDNSDSRTVMRLAVDAAGCPTDCNKLKYSCARVNSISSELSFGSYSCIMRVAKSSSVINVCSIQNGGIGDGASEVYMKYQYPYFTLGYITFGKQKTVIDPIRKDIDINGYHNFTISYRNTSVTWFLNGLELTQRGGSIPVPPMSFYAALIPDPTKDYNASLVAEALVDTLSYQSRCDQIYKYDDYSLRSIPPYYRENCTTSREVNIFNDTLDEDWRYLCDSYTAVPDNTTAEFQRHGTGVMAFEIQQESKPFNLLSKKYILIRQHKYITFWINGGVDGNQDIKLELYNTKYSTRGSIASVSLSTFLKGGLKPRTWNKVIISMREFQVNPSNITTFNAFSFRGGHDEYMGVVYIDDMKFANSTACINDKVAYYKNGELQNGADKTYSAGAVRFNSKDTPYRDVFKKPGNSTDTISFPIISSTSLNLNFQNGTIKANEYYAFQISFYYTQTSSYGKSSSESDLDIPPLSLQVCIQIGDVFPPCIGLSEYMGGKFPSGVWVKLTIPFGDLSAWDDAEISSLIMKTSENNYQGKMAIGSIEVGKRADPPIRSDLESIAVKTLPFNFTLVLSLSLLLFLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUF9</accession>
    <name>A0A8J4PUF9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000190">
        <property type="protein sequence ID" value="KAF2073671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUF9"/>
    <dbReference type="OrthoDB" id="20025at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="121" mass="14194" checksum="8600C3D6584E5A5E" modified="2022-05-25" version="1">MSNKIIRQQLNILNGEDLKVNNRLQKLKSPLGMISLCYCRSYKIIQNKQEETDILAENLKLLRKKAIVRKESKAAQKKRSIISENIDKENQKIVDKKKLHKKLVIKKKKEKRLRNTLDSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUI8</accession>
    <name>A0A8J4PUI8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cationic amino acid transporter C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_003558</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000113">
        <property type="protein sequence ID" value="KAF2075123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUI8"/>
    <dbReference type="OrthoDB" id="5982228at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029485">
        <property type="entry name" value="CAT_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243:SF36">
        <property type="entry name" value="CATIONIC AMINO ACID TRANSPORTER C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER YGJI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13906">
        <property type="entry name" value="AA_permease_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="310"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="387"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="420"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="445"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="476"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="501"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="Cationic amino acid transporter C-terminal" evidence="7">
        <location>
            <begin position="474"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="528"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="528"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2075123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="549" mass="60041" checksum="419E3111C9B811C0" modified="2022-05-25" version="1">MSNQPLIKKRSVYSLNSLLRKYPLEVEQSQENLNKCLTIVDIISYGVGSTVGAGVFVSIGIAISQKAGPGTLLSFLFSALACLISAFCYSEFSTRIPVSGSAYTFAYVSLGEFAGWFVGWNLTLEYAISASAVARGWAGYFQEIFVIFGHEAPEWITGISINSFINISPLAPIIIIICTIILVFGIKDSSRFNVIITMINIITILFFIITGSIYIDVKNWTPFLPFGMKGVFQGCSIIFFSYVGFDSVTTLAGEVKNPKRDLPIGIVGTLVIATTLYIGVTLVLSGMVNYVNVNVNSPLSSPFENRGMKWAAIILAIGTLTSLTASTLCCLLGQPRIYLQMAKDGLFFKKFASVNEKTQTPVFGTIFTGVFASILAIILDLDQLTNMISVGTLMAFTVVCAGVVIMRFRDENGVEHSKIPSAILLFVLYIFASLFGVSSANDWNIGIQIAFCVPMLAIMIGLCFKKQINIPTTFKCPFNPVVPCLGIIVNSYFIMHLTLDSLYRVLIWTVAGAIIYFAYGIRNSKLNESTQTPQSDSDIGYSNKGINYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PV98</accession>
    <name>A0A8J4PV98_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004028</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000135">
        <property type="protein sequence ID" value="KAF2074678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PV98"/>
    <dbReference type="OrthoDB" id="18340at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="213"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="303" mass="34543" checksum="6BF5FD52CE13D185" modified="2022-05-25" version="1">MTYIPASSLQTNNIKSDKEIAKEYADEANKIIASFASNIKQQEAEQDRQQVKQLEKDLENSNITGEKETNKPSSDTTKDDAMDSEDDEEEQENNNEGELIEKATQLFTKALGYDEKCLDAYLGLCYIKGLAGEIQESKSLLDKAESFYPNDERINLMKSLLKEETSSSAHQHTDSCCSKDEGDEFEGVIDIGQKIDIPLLIHNGSVTKKFVYVLREIFDRFDENKDECLNKREIQLFSKAVNNQELDAYSLNSILKNYNSTKKGGLTFLGFVEFYINQTCEDSLETWKDLKTMGYNQELKLMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVI5</accession>
    <name>A0A8J4PVI5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_004266</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000148">
        <property type="protein sequence ID" value="KAF2074410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVI5"/>
    <dbReference type="OrthoDB" id="10266508at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF53">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE M-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="597"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="187"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="230"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="384"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="547"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="196"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="230"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2074410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="726" mass="80253" checksum="9AACC10DDB97F269" modified="2022-05-25" version="1">MATNKIVAILIDNKNKVIKDINNLFIQMDIIDTLCISSTTLVESFESKNQNYLNPILESHPKIQINSSSSSSSSNSNSSGDDLIILAYIGLPTNTGDLSASDIQFLTQCISSLQEGLNQFQKRIHGLVVCTNTPTPSVTHTRRIEKILASKLFKSYWCVSDTPPRNIESFQQILSLIYPNNKLISPSPNSPSIKENNSSNSTSDNNNPSPIQSPMCIDKLKSPLINYNFNVNHQSKNNHSGGGNKAGSNSEDNDQERILPVYKTILSTEEPQQTLPSPPLHPVAMNIPSPTTVAPLSTPNFSSNYNERLLNNINLRKYSPIDTNQNITIVTPPQQSQQQPILINNNSNTSKSDLLSILNDDYKSPLLPFPNSLTTKELLNQQQQFDQQQQQQQQQQQEEQQQQQLVNEIEQKPPLDEILINSNNNGMTQSTTSAAATTNNIISELENNCSSKQKQKNMFEGIPFFKRNDDISSTSTASGSTSCASLDQDNCLNYRNRRSLLPTLGKIDQITKQLISNTYKTNQEGYSFSQTASIALPTNMVVATIQSSKSNNGSNNNNSGSTDGSPCSSPQSSLSYYIQNEAGSSPILGSSNGYGPDVMVVEDNDLNAGFLLKLLEKFGVLNARCLWVTDGLQAVQSFKSSRNPFRLILMDLFMPIMDGYEATNIIRQLEGDFKHTPIVTVTANVTKGAKEKCLTSGFDGYIAKPIRADELKHVVDRYVHFLNLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVI9</accession>
    <name>A0A8J4PVI9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Polyketide cyclase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004212</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000145">
        <property type="protein sequence ID" value="KAF2074467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVI9"/>
    <dbReference type="OrthoDB" id="2586183at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="181" mass="20846" checksum="8D3FB56C3F642EC9" modified="2022-05-25" version="1">MSNPVLKEEHMDSIHWPEKYLPGTTDNYCCNEVIVQGLKIQDAWPYLIKAPCWTQYYHNSADIKFDNQDGPYLYDGCKFFFRTFGFPIDAKVTEFLPSDGNGSTARISWHGWNEDPENRLDVIHAWLFQELSGNRLRILTQESQIGKPAIELATKKPNAMINGHQDWLDGIVKAAKEKLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVY1</accession>
    <name>A0A8J4PVY1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TraB family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003675</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000118">
        <property type="protein sequence ID" value="KAF2075023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVY1"/>
    <dbReference type="OrthoDB" id="48306at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14726">
        <property type="entry name" value="TraB_PrgY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002816">
        <property type="entry name" value="TraB/PrgY/GumN_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046345">
        <property type="entry name" value="TraB_PrgY-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21530">
        <property type="entry name" value="PHEROMONE SHUTDOWN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21530:SF7">
        <property type="entry name" value="TRAB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01963">
        <property type="entry name" value="TraB_PrgY_gumN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2075023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="373" mass="42487" checksum="582C74164FECB889" modified="2022-05-25" version="1">MIVNRFNKLIPPQLPTPFSIYHSHSFSSISSISNCSNTTVHNHPCPLGNKKNNNRTLLSQTSNSQLSFQYAQKRYYNTSSSSLSTEISDDPIYFHRDSNKDIYIIGTSHVSKKSAEAVRNLIRDVKPDTIVVELCQARYQKLKAESEIKNIETRRPQKSFQSFLMEIMDMIRSGNIDPSLILQKTMQNFYNSFRMLGIIPGLEFKYAIQEAERLNANVVLGDVDISVTMRKLSKALLSEMMVTMTKHSYESQRLNDLLEPLANVLKKDNITEEELDREFKLILNNTKLKEMRSLFQSLFPMTFEAMMEGREQYIAKQIKTSKGRSVVAVVGALHVEGIKGLLNLPADKIPEPLPAPPQNLSIQDALSSFRKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PW19</accession>
    <name>A0A8J4PW19_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000136">
        <property type="protein sequence ID" value="KAF2074661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PW19"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="73" mass="7953" checksum="A527096C475F0A6C" modified="2022-05-25" version="1">MNRQSFYAMGLVGAGTMVILAGTPICRPTFGAFLGANKDQQDGIDKIKAVIYSNPLDLAQNLHHHDKVNHHGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWF4</accession>
    <name>A0A8J4PWF4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">O-methyltransferase family 3 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_003677</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000118">
        <property type="protein sequence ID" value="KAF2075025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWF4"/>
    <dbReference type="OrthoDB" id="10251242at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050362">
        <property type="entry name" value="Cation-dep_OMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002935">
        <property type="entry name" value="SAM_O-MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10509:SF14">
        <property type="entry name" value="CAFFEOYL-COA O-METHYLTRANSFERASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10509">
        <property type="entry name" value="O-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01596">
        <property type="entry name" value="Methyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51682">
        <property type="entry name" value="SAM_OMT_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2075025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="225" mass="25052" checksum="847AFFBD90C26A21" modified="2022-05-25" version="1">MPYDGKLVKSHITEESLEYLQNKCLPSFNQSQIELNNFTQQHARSTMQTTPEQVTFFQFLLKVLNAKKVIEVGVFTGYCTLAMALALPEDGKVVGCDVSHEFTNHAQPYWEKAGVAKKIDLRIQPATKTLQDLIDAGESGTYDFIFIDADKVSYVEYYKLSVQLIRPGGIVAIDNVLFHGTVADPAINDDNTVAIRKLNELIQNDSAVFKSTLPIADGVTFVTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWI2</accession>
    <name>A0A8J4PWI2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CMP/dCMP-type deaminase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_003732</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000121">
        <property type="protein sequence ID" value="KAF2074946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWI2"/>
    <dbReference type="OrthoDB" id="1701769at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052717">
        <property type="term" value="F:tRNA-specific adenosine-34 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002100">
        <property type="term" value="P:tRNA wobble adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01285">
        <property type="entry name" value="nucleoside_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079">
        <property type="entry name" value="CYTOSINE DEAMINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079:SF149">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="2">
        <location>
            <begin position="22"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="277" mass="31059" checksum="0AB875BFE7B4839B" modified="2022-05-25" version="1">MSDLFTCKTIEELDENEKKDYEKHCTFMRAAIEEGEKAFIEGEVPVACVIVHNDTIISRGSNKTNIKKNGTRHAELEAFDQIFLNKELNEKFKDVLLQECTLYVTVEPCLMCAAALQLVKIKKVYFGCFNEKFGGNGSVVPLNKSELSNGRPYSSISGLLKNEAIFLLQKFYNQENKKAPVPNKRKRLDPEELLKLAPTPFLSTNLTTSSTTTATKSTKTITTTPTTTTSTLTKSTKTTTMTTTINSMNSEIDSEKQEITQIQLEEQTKSIHCDTSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PWQ4</accession>
    <name>A0A8J4PWQ4_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">leucine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Leucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_003453</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000108">
        <property type="protein sequence ID" value="KAF2075235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PWQ4"/>
    <dbReference type="OrthoDB" id="10249672at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07959">
        <property type="entry name" value="Anticodon_Ia_Leu_AEc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.740.10:FF:000001">
        <property type="entry name" value="Leucine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004493">
        <property type="entry name" value="Leu-tRNA-synth_Ia_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055416">
        <property type="entry name" value="RBD_LARS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00395">
        <property type="entry name" value="leuS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794:SF1">
        <property type="entry name" value="LEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794">
        <property type="entry name" value="LEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24810">
        <property type="entry name" value="RBD_LARS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="10">
        <location>
            <begin position="16"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="10">
        <location>
            <begin position="175"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="11">
        <location>
            <begin position="793"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine--tRNA ligase RagD-binding" evidence="12">
        <location>
            <begin position="934"/>
            <end position="1007"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF24810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2075235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1062" mass="120466" checksum="CE542C979938C4FB" modified="2022-05-25" version="1">MSTVKLDYVREYEIAIQKEWAEKKVFEMDANDDTVETKDKYMATFPYPYMNGRLHIGHVFTITKAEFMCQYQRLKGKRVLFPFAFHCTGMPIKVCADKLKKEMELYGNPPVFPDPSTVEENKPAAAAVKEDPLQFKAKKTKALAKGGGMSYQWKIMQSLGISDEEIPKFADSAYWLNYFPPHCENDLKQLGVGVDWRRSFITTDVNQYYDSFVRWQFETLKDLGKVKYGKRYSIWSTIDDQQCADHERAQGEGVAPQNYTLIKLQVIDPVPEVLKPIADSGKKIYLVPGTLRPETMYGQTNCWILPEGKYGAFEMANGDVFVCTERSVRNMAYQQLTHAKGQYKTLATFKGADILGAALKAPLAVNPVVYVLPMLSIDENKGTGVVTSVPSDSPDDYASLQDLKLKAPLRAKFGIKDEWVLPFEVIPIIDIPGYSNTSAVKAYTDLNIKSQNDRVLLDQAKDITYTKGFNEGIMMVGEYAGKKVSDVKKTIKDELIKNGEAVEYSEPAGKVVSRSGDECVVALSDQWYINYGEDDMEWKNKTLNVLSQLETFGPETKKKFEYSLGWLNQWACSRSFGLGTKLPWDEKFLIESLSDSTIYMAFYTIAHHLQGDVNGTKPGISNIKPEQLNRAVWDFILLDKPYPENCSIPKEKLEVLKKEFNYWYPVDLRVSGIDLVQNHLSFFLYTHAAIFNDKQQPQAIRANGFVNLNGEKMSKSTGNFLTLYDSIKQYSSDGTRIALADAGDGIDDANFVNKTAESALLKIHTQNLWIKEALDNIDQYVQGKPDRIQDIVFDSEMNRIIAGAEQAYENTQFREALHLVFFDLQNVRDQYKTATLDQMNKSLILKFIEIQALLIFPIAPHFAQHIWTMLGKGSILDARFPVAGPIDYEALRKNQYIHNTIYNFRMKLQLYNKLKLKGKPPGTKVVPDASTIFISKSYPKWQYETLTYLQSIYDEKTKSFTKDNNAISEDLKKNEDIKPQFKHVMGFVASLLQDVKTQGKEAIQTTTSFDEAELLNQNLDYICKTLEIPTFDVKEYTPEAAAAAGGKTNVVPVPGKPTYNVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PX06</accession>
    <name>A0A8J4PX06_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005186</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000200">
        <property type="protein sequence ID" value="KAF2073509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PX06"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="649" mass="75880" checksum="318818051DFE6F10" modified="2022-05-25" version="1">MDHEYVDEIIKQDVEYYHDLKQDIINKFDTLQTTLIDTVTQQISIAKSNAIKTLDQHCDKEILNALKPNINKMMGPILMLNIILNSSFLKMEIDAHQITSIREKKYVLSQTSPDINKLRLFIQQVVDNIKLELFTNFELFIPIKKEIIYEPFQSIELVNFLLKHQEQDKKEEKEGPTILKNSERIFTINGLMGTGKRTRLLSFMSTLQIKYKSVVIVDNISAMDSKSTLSDDQDMRDIEEGTLYNILQRIVRLVKPGEKEKVLLINHKGISDYDMGIINKNPNFIIINITENEKQELNMQPLSYVYCVKLFNHFLNSQLDDQVLYYLIKTYCRQRSKLIFQTASSINNGTKLGFEESPFDFNYFMLKGLDGFISNYCYPLGRYVCFTILSSELLLKFVIYLGAPRFRELKFLFRLFAENIKPIMSISICTSFSPLVDKKENYTNLSFFESFSKNTFTQTNNEIKVKLKRLNLDSNMEFNIDNCPAINYKRLQQFIHLVPITGSLKIKNQEYIQQDMPPIFGRIEWGDFIFIKKYTEINSWGRTKQNQLIAIHLSLNPYDPTNFIIIGDIRHYLDPIFISILNNNGIFIKIESLYNRFTLLFKFKDQRINEINRGTYLGSLSGVITFKDKLQIEPIDIIDFCSDIDFISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PXQ1</accession>
    <name>A0A8J4PXQ1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Synaptobrevin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_003937</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Endomembrane system</location>
            <topology evidence="9">Lipid-anchor</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptobrevin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000131">
        <property type="protein sequence ID" value="KAF2074749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PXQ1"/>
    <dbReference type="OrthoDB" id="27923at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45806">
        <property type="entry name" value="SYNAPTOBREVIN HOMOLOG YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45806:SF2">
        <property type="entry name" value="SYNAPTOBREVIN HOMOLOG YKT6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0564">Palmitate</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Longin" evidence="11">
        <location>
            <begin position="7"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="12">
        <location>
            <begin position="142"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2074749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="22660" checksum="300EB439EF87E36E" modified="2022-05-25" version="1">MKVYSIGIFKVTPGGKPVLLSIVYELSSFGFFQRGSVKEVSLFVSRETVGRTGVNERVSMEHTQTQKVCHTTVDSKGLGCTVLTDSEYPGRVAHSLIRLALDEFNKVHNESVWRAINTDTELPTPALEQLILKYQNPHEADPMMNLQKNLDETITIVKKTVEQLGQRGEKLEDIASKSDDLSFQSKAFMQNAERMNKCCGYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PY03</accession>
    <name>A0A8J4PY03_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">BUB1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_002754</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
            <location evidence="1">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000079">
        <property type="protein sequence ID" value="KAF2075951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PY03"/>
    <dbReference type="OrthoDB" id="248495at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051754">
        <property type="term" value="P:meiotic sister chromatid cohesion, centromeric"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22249">
        <property type="entry name" value="UDM1_RNF168_RNF169-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.430:FF:000003">
        <property type="entry name" value="Checkpoint serine/threonine-protein kinase BUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015661">
        <property type="entry name" value="Bub1/Mad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013212">
        <property type="entry name" value="Mad3/Bub1_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14030:SF4">
        <property type="entry name" value="BUB1 KINASE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14030">
        <property type="entry name" value="MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08311">
        <property type="entry name" value="Mad3_BUB1_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00777">
        <property type="entry name" value="Mad3_BUB1_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51489">
        <property type="entry name" value="BUB1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0137">Centromere</keyword>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="3" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BUB1 N-terminal" evidence="7">
        <location>
            <begin position="53"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="882"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="198"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="236"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="312"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="396"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="489"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="528"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="724"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="271"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="337"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="396"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="500"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2075951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1208" mass="136574" checksum="632B810706D15375" modified="2022-05-25" version="1">MSSSTTSLSDWENVKENILPLKTGRDPTKLATFARTSVGGLSNEDLEKEKEKYETLIKEYTGDDPMDLWLKYVKWVQQSYPGGNMKTELIKILERCTKLFYSYEKYKNDHRYLRIWITYADMCRDPVEIFAFLENRGIGSSLSLLYEAKAIVFENKGNYQAADEAFKLGIQRKASPLERLQQKHLDFERRLIARIKHQEQQQDGSVSGSMVGDENSTPSTRRAVLGTISGAVVSDRKSSVTKGAPIGGSDKKRKGPEKKSNSEFQIFSDETGGNSSNIETNSKFLGASRKASSSTSGNGGSQIRWEELEPELTKHKENTQIPAKWSETKITQKKQKSGSGTNSSFQVYCDESIETGNGDSRPASPDKLKLSNASKLSQLEQIQNNPLASFPKTLSVSSLSTKPSSSKSSSSRGNEKIGYKKELFINNEQELCFEEYRATLYMKKQNELDQLLLIQKKQQEEAELELKLKQEKEKQELLLKQQQEAELKLKQQQEREQQQNNTTKRNYDSGMTMTIHTKEAFNDVMEMFGGPLGFEDEKNSSSPDPFSRKQPQQQQQQEQEEPSIFSKPISNNIPFNDESELDNQENIDPAHLPRQKITNRDILKQYHQGPEQEQEEEEQYERERIKTMERIDRVVQKEVAAAKIAGNSNFVIFEDPTISSKPTTQTENDVNALRTSTKSTFSIFSDPEPVVQEKKPSSFSIFSDPEPVVQEKKQPSSFTIFSDEPITQEKKQPSSFTIFSDPEPQQQNQQSLSSFSISTISNITSSRFVDVEDQMDTTSDKITNRSIGNITGNLTQNIDPTGVIFCNTIPLDECGDGFNPYDADYQFTLEQTVSPVVQSIDTFYNCSDDVAPISTFLENVEPGNEFDVADDQITVALVDGILSVQSCIGRSNTSQSSTFKAECTSGSYEDKQLVVKIQGNTIWEFYICHQIQSRLNAAGQEKACPKFNQFHSLSYYADKSILLMDYLDQGNLVDVVESNMMDESLAMFYTIDLLKSLESLHSHAKIIHGSITPQSLFFIYGSVVNWPDWSPNCEGAWDTKGFIFTDYSKSIDISLLKSDIKFSCALDQLAPGTSPIWFESRKNSLWSYDIDYLGLAQVVYYILCNGKASPSSPISISKDESGQLYFTDFQSLVDDKIYTQSNALWSNLLSSLLNFNNSVNEQQPTTGSILLTHRTAFEQYLSTDDRAKYIKNNLRKQNLKVFETISKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYB2</accession>
    <name>A0A8J4PYB2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cation efflux protein transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_002529</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000070">
        <property type="protein sequence ID" value="KAF2076176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYB2"/>
    <dbReference type="OrthoDB" id="78669at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904257">
        <property type="term" value="P:zinc ion import into Golgi lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000014">
        <property type="entry name" value="Cation efflux protein/ zinc transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045316">
        <property type="entry name" value="Msc2-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45755">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45755:SF4">
        <property type="entry name" value="ZINC TRANSPORTER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="254"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="380"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="447"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="484"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="556"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="585"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="10">
        <location>
            <begin position="380"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="509"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="513"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2076176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="697" mass="77242" checksum="50FC525FE7D760F4" modified="2022-05-25" version="1">MSGSSFSNNNSISNGFSKYTKNSSQSLIILLFGSKAVRACCTILLYFILQQYSLISTSFYLTLFSSIMFIAYEKPWTLITHGGLRPSQIKKIIYLSLFGIFIQLTWNASIKYIGPTGSILASDYTHSTYPFLFNSILNANYLSADLLRASGMLIAGYLAIPWFTPSSSSSITTNTGYSPFVIFLMGVFFLVLHNILNLIQRLMVKKWSNPGSKDKLHSLATGISTIILFIFKTINDSNESNGTYSNNNITFGQIILFIIISFSCIFYNYFIEAISEQQLGLLVLSKASLTATLLFSIITGLFTGYTFFHPILIISFILILTSIHIIFSKDTDVLQQAQGKGGALNFSGSASGHHNRDGHYFSIVKDILGQIIEKPTSRRIFTFLIINLMFMFVEMTYGIWTNSLGLITDACHMLFDATALFIALVAEVISQWKKNDTYSYGYGRFQVLSGFVNGIFLIFISITILMESIERLMEPPEINTDKLLLVSVLGFIVNLVGIFSFHGDHGHSHGGHSHGGGGGHSHGGGHSHGGHSESNHGGLLGTEEPKKKRNVNIDGVFLHLLADTLGSVGVIVSSLIIQIWGYTMADPICSLCISILIFLSVLPLIKTTGKTLLQCTPESIEPNLYTINQKISNVQGVTQLVNYHYWSHYDEMNVATLKLSITSDANQQKVRKAISKILKEYSIQVTSIEFILPIEYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PYE0</accession>
    <name>A0A8J4PYE0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="10">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_003447</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="10">The helicase domain is involved in the stimulation of RELA transcriptional activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DEAD box helicase family. DDX1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="10"/>
    <dbReference type="EMBL" id="AJWJ01000108">
        <property type="protein sequence ID" value="KAF2075229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PYE0"/>
    <dbReference type="OrthoDB" id="1735at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12873">
        <property type="entry name" value="SPRY_DDX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000076">
        <property type="entry name" value="ATP-dependent RNA helicase DDX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="15">
        <location>
            <begin position="2"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="12">
        <location>
            <begin position="71"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="297"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="507"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="264"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="9">
        <location>
            <begin position="2"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="287"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2075229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="755" mass="84574" checksum="F9101AD8E78A6298" modified="2022-05-25" version="1">MSAFEDLGVLPEIIQAVEELDWTLPTPIQAEAIPLILGGGDVLAAAETGSGKTGAFALPILQITYETLNNKAVVNIPIEEKKQDEQEQESASVKWNLNDRDKDLAIDGLVCQSRSPDWSGVRATKGISSGKYYYEATVRDEGLCRVGWALKKSTRNIGTDKFSWGYGGTGKKSTESKFVDYGKPFGTNDVIGCYIDFDQEIMGFTKNGENFGTAFQFNSKLGIFYPALLLKNAEMGFNFGESPMKHQLSGFKPLSEAELLEESNVVVPTTTNNDKKKNNNNKKQQKQQEQNDNSSNINKKRTPLSLIIEPTRELADQTYSAILNFSKYLLEPKIEVSLCIGGFDTKRSAGSADGDIVIGTPGRLESLVKEGAIDLTNIKFFVLDEADQLIDENLAIVNFIYNKLPINNLQVLFFSATLHSPKVVKFCEQITKNPTWVDLKGKDFIPDLITHAYVKADPEAMQPLWRDSGNPLRMKTDGVHNQDVKEFGKLKTDEQKSEAIKLLKAQLLLKVIKSFNMDQAIIFARTRLDCDHVYEFLNAAGGRLNDKALEGDYPCAVLHSDKATNVRKENLEKFKSGEIKFLICTDVAARGIDVRGLPFVINYTLPDNFEDYIHRVGRVGRADRIGLAVSIIGAHQEKVWYHINCRNKGKGCFNTDLVEQRGCCIWYDEPSLFQPMLENLGPVELNAEFKLPEHLAESKFGNFKDRNAKKHLEYQPHTEQLKSRVIELAHLEETIQVDFLSLPKRVLSKVDSMST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZ98</accession>
    <name>A0A8J4PZ98_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006685</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000319">
        <property type="protein sequence ID" value="KAF2071996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZ98"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2071996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="158" mass="18558" checksum="82011148AD1EC10A" modified="2022-05-25" version="1">MDSLLIGFQQLLQKECEETLISCPFDYFGCKTTFHKEYLNIHLKSDEHIYHCYDVLDRIVSVTKLERGNCKTTFQFLGVEALFSYNIHETTICAMVFNLENQPFAKDYEFSINLYDINNCRLNKGQTEGLADSKWALFMDPNFIHKFHSITLTIFKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZS1</accession>
    <name>A0A8J4PZS1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mannosyltransferase</fullName>
            <ecNumber evidence="8">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">CYY_001648</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Endoplasmic reticulum membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the glycosyltransferase 22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="8"/>
    <dbReference type="EMBL" id="AJWJ01000041">
        <property type="protein sequence ID" value="KAF2077016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZS1"/>
    <dbReference type="OrthoDB" id="416834at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF4">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="115"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="258"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="293"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="317"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAF2077016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="519" mass="61162" checksum="CD844733426AC0B2" modified="2022-05-25" version="1">MQKVIQCIDALLINTFFDPDEYWQSLEVAHNLVFGYGYMTWEWKEMIRSFLHPLIFVVTFKLLDILHLDTTYLMIVVPKLVQGIISAIGDYYLYKLSIQIFNKQVAKWALVCQLVSWYMFVCIVRTYSNSIECVLFIVSLYYFPLYEKLPQYQKHSKPKLSIALTAIAFLIRPTTAIMWIYILPYHIVSLYNKHKQHDAIKRILKFIIIDVLCTGIIILLISLCIDYYFYGQLVLVPYNFLYFNVIKNVSSFYGTHPFHWYFSQGLPAIAFTSLLFFVLAIKKLWSNGVDKQQLNLSFLTIFTVFCYSLLGHKEFRFILPILPIVMMYSGYYINSTLTSNHSNSNKSKSTQQTNEKSMPSFWIIFLLLLNIPMTVFFSNFHQRSPIDIMHYVNQEISPLATIDNPISIHLLMSCHATPHQSYIHNPNIDFKLLECPPPLNANDPIDEKTEFFNDPLLFMEKHYNQDKDYQFPNYFIIRDDLIDTLDQYLVLNHYKSIHTFNFPLDITSSIVYVRQKQFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PZX6</accession>
    <name>A0A8J4PZX6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_002116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000056">
        <property type="protein sequence ID" value="KAF2076565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PZX6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2076565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="160" mass="18520" checksum="8CBE975DCD39939C" modified="2022-05-25" version="1">MNYHLQQTNNNDSNNDTENDNDNLNNYDINDNNDNSNNNHNKSCSCSYIISKMKDLYSSSSSLLSLYRNYIDIKSQDNKTTITFVSDGGSQTEEAYGIIDGIIDKNSCCSYCYYYNFIIDHQLLQSKNRHNTLKRNSDLILLIHNEDKDSFSVNNSFVYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q051</accession>
    <name>A0A8J4Q051_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_001977</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat SWD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000051">
        <property type="protein sequence ID" value="KAF2076720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q051"/>
    <dbReference type="OrthoDB" id="27537at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037867">
        <property type="entry name" value="Swd2/WDR82"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19861:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19861">
        <property type="entry name" value="WD40 REPEAT PROTEIN SWD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="4 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="59"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="191"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="233"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2076720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="30522" checksum="9C636E85187EF9FF" modified="2022-05-25" version="1">MYVHQSEYRLQKTLYSKKYGVDLIRFTHTNNSIICVSKNSWDESLRYLSLHDNRYLRYFKGHRNKVVSLSMAPKDDMFISGSLDDTIRMWDLRTNICQGLLRRNGRPSVSFDPEGMIFATAVSVNTLKLYDLRNYDKGPFLSVVVDHPTPVEWTTMKFSNDGKYILLSTKENVIFLIDSFTGEKKQTYTSFVNDNQSVIEASFTPDAQYVLSGSEDGTIHVWKTTTGEEVAVWGGHSSTVGCVQWNPRSMMAASADSTLAFWVPSITE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0N7</accession>
    <name>A0A8J4Q0N7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_001079</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class IV-like SAM-binding methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000024">
        <property type="protein sequence ID" value="KAF2077616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0N7"/>
    <dbReference type="OrthoDB" id="361029at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18086">
        <property type="entry name" value="HsC9orf114-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003750">
        <property type="entry name" value="Put_MeTrfase-C9orf114-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12150">
        <property type="entry name" value="CLASS IV SAM-BINDING METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12150:SF13">
        <property type="entry name" value="METHYLTRANSFERASE C9ORF114-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02598">
        <property type="entry name" value="Methyltrn_RNA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="125"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="52"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="459" mass="52466" checksum="382F4A36BEA770C3" modified="2022-05-25" version="1">MKTTKLSDPRDGSKKNKRKVNNNGSSAGVAADKFNSMLSFFEQGDQQEQEQPKPSTENVAPAATTEQVSEPIAVVKKAPKRPKRTFIDSVSPSTENNKDLDSTTSTSTSNTTNNNSNNNDKLKEIEDKELDLLIKEKELKKLKKDIKDEKDKININLARQKQLVELNKQSTISLAIPGNILDDITSAEMKTYLIEMISRTIVMHQIDEVIVYKDSNNDKNDQDNINYLMKILEYIETPDYLRENLFYVLDPDYEYVDKLKKMSSCRQQELDDQHRYREGFVINKPHQGKSVVNVGLGKQVLLDKKIKGSVRVTVELSVSFEQQLQDDQCEFIEGRVVSFMDIKEQGYYWGYHVRVADSLAATLQGSPFESKNYDFTCLVSKSGETYPRPLSQQLMTKKYNHLLLILANHIDNAQNEIPAYQDALDLHINTLLNFKPSFPIRFEEDLLITLTKLKGTVIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q0S4</accession>
    <name>A0A8J4Q0S4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_006200</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000275">
        <property type="protein sequence ID" value="KAF2072491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q0S4"/>
    <dbReference type="OrthoDB" id="2335338at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1010">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904:SF523">
        <property type="entry name" value="STRESS-INDUCED-PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904">
        <property type="entry name" value="TPR REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="639"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="408"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="475"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="27"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="280"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="520"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="117"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="165"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="301"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="318"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="350"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="372"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="526"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="592"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2072491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="661" mass="73109" checksum="AA7008DF2E26AEAE" modified="2022-05-25" version="1">MNSDTSSLKIKQEEILDSLDRLDSKLKVLENNDNNMDAVEEQQQQQQEQVEQTKVEEETTSVIQDQVSSVDDAAAEQQQSHGTETNDESSSKSETSTVDEPVAVVEEEKQEEPTPVVEEEEKQVEQDEPVAVVEQEESTPVATQEQEKVEESTPVVEEQEAVVVEEEKQEEKAVVAEEPAVEEQEKVVEEPTPVAEEPAVEEEKVVEEVVQEQAVVEESTPVVEEQVVVVEESTPVVEESAPVVEEQAVVVEESTPVVEEQVVVVEESTPVVEEQAVVVEESTPVVEQEKPTIEEQESTPVTETTTTTTTTTTTVVVEDQKPTESEKPTIEEDSEDEKPQENGNGNQQEQQKEQEQENTQEENKEQEQKQQEEEESSSSDEEQVHEGQEDIEEDDSADVNLLKSSEMANFYKLEGNEYHKNGFYYEAIERYSQAIEISPSHLLYSNRSISNFKVNRYQDSLEDAIESIQIDQSWVKGYHRAGQALEKLKNNEAAMQYYLDGLKIDPKHEAILEAVEHLQKSLSKDSTTSKPTTKTTTTTTTTTTTTSSTTTSAAASTSSTAAATKSTTASSSSKPTTSSTAKKQVPKQRDDESSDDSESESESGSESESGSDSECSDSDSDEIEVEEENTKQLATVGPLQVVGISVIVAFVGVMAYKLFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q106</accession>
    <name>A0A8J4Q106_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000271">
        <property type="protein sequence ID" value="KAF2072536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q106"/>
    <dbReference type="OrthoDB" id="24077at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="60543" checksum="C4A01CF83FDC9F9A" modified="2022-05-25" version="1">MDTLFYFVWKNSFLRSLIRYRVLRDTEINVDNEYLINNHQYMSVLSPQEKYQHNISFQWRISSSKQFTEYLDNAHKHLVDDLDICITNDNADDDDDDQNGRQFTDIDCSAFHDGLHKLSFFIDKYTTGYNKLPDTIVNLNIHASCYTTDRFTSPIVERILGNLPRDLKALCLPLSYTISSLLVLPDNLVEFNYSSAYNDFKQLVVSPNNKDIDGCTLVAQCAQDLEWASEQSWIKSLQIDTPFNLEGKPFPSQIRNLVVNCQIELDIDSFPSTLESLSIFNCDIPSLLPPRLITLSLEDFNERLERGLLPSLLENLSIEMFNVEIEAGALPSSLLKLDLTCFNQPLLAGVLPNGLEDLNFEEYDQPLLAGVLPNSLKYLSSTFFNSVIEPNALPASLTDLGLYSYSGSLDGVGLLKNLKELALLKIDASLLPVITSPSITKLKLSFESIDPSLCLANTFIKYLTLRHNGRMRYKLKANFLPLCLLKLKATKIDIDSSADIIPNGCTSLDLDIDKINLNLNFLPKSVKYINTHEPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1K6</accession>
    <name>A0A8J4Q1K6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_001111</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DEAD box helicase family. DEAH subfamily. DDX15/PRP43 sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="AJWJ01000025">
        <property type="protein sequence ID" value="KAF2077570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1K6"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17973">
        <property type="entry name" value="DEXHc_DHX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000007">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001148">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15/PRP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000003">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044756">
        <property type="entry name" value="DHX15_DEXHc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF109">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="78"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="264"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2077570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="704" mass="80604" checksum="7B2DDCE86B6A686E" modified="2022-05-25" version="1">MSKRKSEMADKDQKKKLKETTTTTTTTNTDDESTKTTTNTTLTAEEWIPKKEKYSERYYQILEKRKDLPVWKQKEEFIEKIKSTQVLILVGETGSGKTTQIPQFVCDAGLVKPGTMVGVTQPRRVAATSVAKRVSEEMDYELGQEVGYSIRFEELSSPKTFMKYLTDGMLLRESMNDPLLSKYSVIILDEAHERTLSTDILFGLIKDVIKKRKDLKLIVMSATLESGKFQKYFDNAPLIKVPGRLHKVEIFYTPEPERDYLDAAIRTVINIHEDEDDGDILVFLTGEEEIEDTCAKIQRECRNRNLKPMKTLPLYSSLPIFQQSKIFDTYKERKCIVSTNIAETSLTIDGIVYVVDPGFSKQKTYNPRSRVESLLVAPISKASSNQRAGRAGRTRPGKCFRLYTEAAFKKLEPQTHPEILRSNLSAIILQLLRLGVVDLVHFDFMDPPVPETLMRALEVLHYLGALDDEGNITKVGEIMSEFPLDPQLSKMLIASPEHNCSNEMLTIAAMLSVPNVFVRPKDSREDADAAKKAFDHFDGDHLTLLNVYHAFKQEKEDQTWCYDNFLNHRSIKQADSVRSQLARIMTKFKLPLLSGDINSRAYYNSIKKCLVAGFFMQVAKCEKKNQYFTLGDEQSVIFHPSTGLSRKPEWVIYNEFVLTNQQYIRTLTEVKFEWLVEACPDYFNQGGFPKKSKDAIDRSKRLMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q1S6</accession>
    <name>A0A8J4Q1S6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pmp22 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_001006</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000022">
        <property type="protein sequence ID" value="KAF2077690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q1S6"/>
    <dbReference type="OrthoDB" id="10267969at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF80">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2077690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="22045" checksum="69913E4C653D1920" modified="2022-05-25" version="1">MSRFFRILKSAQSQYISALHNRPVITKAVTSGTLYFISDSISQGIENFNRKDEDKQPFQWARATRMAIFGFFITGPTFHYWYNILDTSFPKKNTRHIIIKSVLDQILCAPLFDAVFFTGLGLLEFKSWAEIKDKLKKDWLFTYLVDCSVWPIFNIFSFKYIPNSQRVTMMNLFNIAWTAFLANTNYSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3F7</accession>
    <name>A0A8J4Q3F7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_000075</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000002">
        <property type="protein sequence ID" value="KAF2078575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3F7"/>
    <dbReference type="OrthoDB" id="1919336at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
        <property type="entry name" value="HMG-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112:SF22">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION FACTOR A, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09011">
        <property type="entry name" value="HMG_box_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="4">
        <location>
            <begin position="51"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="HMG box" evidence="4">
        <location>
            <begin position="118"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="2">
        <location>
            <begin position="51"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="2">
        <location>
            <begin position="118"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="169"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2078575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="199" mass="23383" checksum="46393AA3E8183D23" modified="2022-05-25" version="1">MLKVGQYLYKQNSTGLVLGGGSKQLFSCFTTKAELKEEQMVDKILLKQLKPKRPLSSYTFFVKDYCSNNATTDLSVGERAKVISQTWRLMSPTDKIKYQEKADEYKKSLSNIDIPQKPKKPSGSFYLYVAANRDPTRNYIEQTAELRPRWRDLDEKDKKPFHEEFEKLTNEYNQKLVAYNKKIEEMKNSIKEMRKKKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q3Z1</accession>
    <name>A0A8J4Q3Z1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Right handed beta helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_000336</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000006">
        <property type="protein sequence ID" value="KAF2078352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q3Z1"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31318">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31318:SF1">
        <property type="entry name" value="POLYMORPHIC MEMBRANE PROTEIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035156600" description="Right handed beta helix domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="505"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2078352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="549" mass="61377" checksum="5677928BD70DE743" modified="2022-05-25" version="1">MSRKLQLLFLILFFSLPNSIYGDSNENIGPQSSENVLNSELSFFVLSDPIDNYTAIGCGDSFHNPCNSIQDAIYAFQNKTFFQSNDSNSQVVTLSTPFVLNLFGNTHFKSRTVSLDHLNIKFQSYYSNQVTIEGFPIPVESNFTKEPLFTVEFTDYTNIVFKNIIFSSFESSVVKVFFSNSSILFEKCTFVENTFNTGDGLIDYRSTSSRENKLISFINCLYVGQKGILGSITINNAKLFIFNSTFGSNKNLGFVLKNSDLIIDGSSFSANNIYKQSNGMLQGLFECENCYVTITGSEFINHELDVSLLYFKNGRVDISKSKILNNRNYIYYGDYIPGGLIFAQDLDMRIANSDFNSNTANYSILYSSGCQINIESGHFIDNQSFNKTIISIKDSEAFYKETIIRSSNPQNNIISISNSKATIDQSTILISKSLSKISAIECERSELFFIDSSIENYDNDENLVCNTGCLSYSKGNSTFTTVKRGKIQSSNSLLCPPKVRKLNGGAIAAIIVCVTAFIVIVVGVVFILKRKNIFIKQNYGQLSDDETPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q405</accession>
    <name>A0A8J4Q405_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SecA family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_000941</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000020">
        <property type="protein sequence ID" value="KAF2077754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="21121at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017038">
        <property type="term" value="P:protein import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011115">
        <property type="entry name" value="SecA_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014018">
        <property type="entry name" value="SecA_motor_DEAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF194">
        <property type="entry name" value="INVERSIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07517">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SMART" id="SM00957">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51196">
        <property type="entry name" value="SECA_MOTOR_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="1100"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="1168"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="1232"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="2039"/>
            <end position="2071"/>
        </location>
    </feature>
    <feature type="domain" description="SecA family profile" evidence="5">
        <location>
            <begin position="2936"/>
            <end position="3268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1007"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="118"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="168"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1007"/>
            <end position="1025"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2077754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="3268" mass="370212" checksum="D2698811F26324E8" modified="2022-05-25" version="1">MDIIGYKAHRVAPRQEASWRQTQSAVMREEARNIALGGSGFTKGLFTERERQKLAEWHRTGKFKCDITGKTYESGYKVKVALNRARANEDKINFHHINCEKKNPHINKDPRNIRLLTRNDHYQSKQGHRGESTKLPTDGRFIDREKTNDLLEKQFQQNLKSSGLEKSYNGSNPDSQAQHFSSGTKGGALAGEIGGVAVTSADIFKGWEKNINQFNQPIHTICIEKDKFDLKPEDWNSDDFSQIMIELSNAIYQHGTIPFFSLHFNQDGSMYTVLHPAYHNTLVGKVILILDYYMKGFINGGFLPEEFALRWSDGSRSLDRSTLKLHMIDIKEEISNGRWKNIAGLEDYKSLRERAFEMGLEGTEGVENIEKKIQQEFSESNAVNSPAIDALRVAYENLKQHSGLKYQTSFRILASERSAQCVSGVWMLDGDFEVKYTIEPTQPYQQYMDAYQTVFGTLPLEYLVQEELYKEAAEAIRDQMPLLPPCKRYFQLLKQISFLSSYLTALKSSGQVPLIAKIDTNVLPNEIDKTPNLLPPLPVRYYEAVKLSLTVDEIISEMQKDSALISKFYRYLDSHLAPDNGFDANMEDLMVREIQPKVISLLNKKLLSLGASGNINKSGKPIVLSFIQLFGTFVGVRTSQLYSSLLKNIMKPMIEVLERYNEIAANYQAPWPSSQEIENDEDESVTKIFQDKLNQYAISTKDLQASAIEILNSCKNHIISEMCSGCHGNITSHRANVKVNGSPCRNQFVNYLTEQFTTLHEELKEKYEKEGFFTKLFSSSKSEMNKFKLILDGMVNNNMLLINELIKIALPGWEEAVKGKLSAMTPLVDATNEFFEEQLVNFGKSTTTVLRERLGTLDILSIFDERQSHILSLLSKKTKNVSVHNNTVGSIINRLKSLGSALSSMMSMLNGGKSEGGVLPISKLILVPDKDYQSKGHQLVWGGCGMVLNQLEIQSTFPSKSLDQSLDFAEHVKSFLNQKLYQWVHLADGPTNYSAFSFKVIPMDQQDGSEIEQDEDEDEKDEEDNLTSNLQECANMVASTNMDEKSFIAMLNGNKAILSQRDQFGNSILHFLSAKIGVSHLIKSLVSRMGSEFLNEKNSYDMTPISISILMNQFSNVKVLLESGANINIEYHNSMFPLLSAVLEGHSDVAILLSKYCKVEEINRQLHEGDTSLHVALMTGQIEVANQLCRKGASVEIVRKRDGFTAIEVAAQLSIIDFIKSVPFEKDRALPSQGTALHVAVENNSFKIVEFLLQKGWSVTIQQLNGETPLILALDKGFPEIANLILTFSGDSSNVNPLPPPTKEEAARPGYRASQCIPTSISKYRTYINSKKQTAAMIAVSKGYIDIAWNLISRNKESWVHKDQYDNNLLDYIILYGESRLLEKVIQSKMFSLKLDESLPNNRLSYLAWSLMQKKIELADILLENGAKYFHPDPQWTIGASAVRANHSGLLGKLMEPSSTNIFNRVEPFNIESLVYEKNLLEIAAECGSLECLEIIYNKIKSKQGMESKNFLLHQGTELLYYALVGRSKKVIEYLIRWYHDLNIQLNIQGDYVVGILLSTDQMSLLPLFQNRGLKLNNDIVGVCIVDSAIKLDKLESIQRYIKEANLELLNQKKDPLGSLFYYNLGLRAIEGEAKGILSHYQDEISSAFSNVCPTSSEFTVLTINVAEPLFAKLVLNFVDDEKDLEVIRKLIALPNISEEMLFKAVHHTLKIDNAKLGAVLLAKMKSKLSQKFKFYNDKPPISILELFQKLKAFNCWKLIPIPDWYRDSYGTEEYRPDPEIIKSLNENILIGDDISILNTLKQLTQLNKITLHDEKKVPLLHAIFLLGSEELFKYCIAQGCDPFALFMKFNILLNLGSDASKLGQLLSILEKNFTTEQLEVLFYQEFEDRGIVATHIDLLLSIFTPSKLREAYQSAKMGSNPNYSKYVIRFANTLGVVIDEKKLTSEMGDEVEKVFKLVTPTNRKLAKNQKFIAPDYFVKPTNKILQSMIQNVSQPSFIFEWFPNEIDLKITDKTDIIQLERQYHHLQMIKVIDCRDKDGCTPLMKACLMGENQAVTRLLQYGADPSLLDNEGNSALSYALQKTNKNPSVTINILIPYSTSTLSIANNKGILPISIMTHMSLSNQVSQLLMKGGFIDQDGKYNHYCVNQSIYNSGFEASKTIKLLLENQRLYSPGGNQEQSGPASYRAQELASHSNNLNNLNSLIEESGSFVDTFDLHLGDHGSPLDIAIQNNYSILINSLTRCPDDSNLRWVKVIQSDNLNNLELLNSHFELYGIIPLAKLNALHIAAYFNCHNLTRYLYVMGVSINEVCEIESTTPLQLAAFCNNPMFIKSLINSIRSNSTNNEVVDGILKPSGLSGGRSALQIALCNDHFVSAYLLMQASSDVLEGKPSIQLLNYFSANPHSIPFSNIAKMLKYLIIFGDNSLIDSLDTKYISPDIGSFISKMVLNVTHWNNRLSFESKLKRAEQLGFTTRQSYDILNSEWLVAFSKTDTQINTAPDEPLQQIKMTAIENDRYKPLFEVVKLILNDKSIFNLVEFVYSKSSSEITVGQLQKSLQCFKSIYDSPNRTSIVPLFDKAIARFWPWEILLKTTIFNLITDIIESNSIQLWNWFISHFTLISTRSIAEANVASAIKRFREFMQIIQHQCKNNISTLKSLSNPPTDLQLSFQSESGVSNMELAILSLGRQEHLRFIQASVLHNLPKNREYIQYSIERAKKDSLLVCRQVFEISNIDLYQQQTNSLQVASACGGAIIETFRGLLSLGLSSLNPIHATFINNFITNIRILQMTDNTTVPVGCKLLLVQYFMSILSNNKFNVISKSDYLDSSRIEWCREMQKIISLVPQLLKSIGITYFKRFVAINLPQQTGLKLFEKQVGSLREFIELSDNILSSWAEKIPRSKIWNLNNYQSLKSVFKEEQDKIIKLKMNQRPFSEVLQSFQSPINKNPLEQQELAIIQKLFTIVEQLQDKHKDLSQQQLISICINSANQLRSKPLSNVNVEIISALRWLIYHQFGIFPYSTQVCCVLGLLLYKENKDLRGRIAQVLTGEGKSTIVTLLSAYCGLAGKQIDVITTSEYLAYRDFIKYEPFYQLLGISCSQITGNHPEESAFDAQILFGTNSNYEFALLRDKLYGSKNLFTNGNPREKDIVIVDEVDSLFIDSAFNSARMSIPSQEHHGFIYQPLSEYVKNNIVSHIVIDEKLSEEEQELEISKDITPQHIADARFILEKEVRFSPMKKRPRRKHLNGLNLWEMKGLERSSPVQSSRSTSKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4L7</accession>
    <name>A0A8J4Q4L7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA recombination and repair protein Rad51-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000528</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000010">
        <property type="protein sequence ID" value="KAF2078144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4L7"/>
    <dbReference type="OrthoDB" id="420422at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033063">
        <property type="term" value="C:Rad51B-Rad51C-Rad51D-XRCC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042148">
        <property type="term" value="P:DNA strand invasion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19490">
        <property type="entry name" value="XRCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030547">
        <property type="entry name" value="XRCC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46644">
        <property type="entry name" value="DNA REPAIR PROTEIN XRCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46644:SF2">
        <property type="entry name" value="DNA REPAIR PROTEIN XRCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2078144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="283" mass="32888" checksum="737BF9B0CD675612" modified="2022-05-25" version="1">MNDSSYDATAFNIFYKREKKTVITGIQDIDQYISKSNQSSNVQQSFTHTGGVIELYGSSGTRKSETLLYILIQCLLPSYPPFNGNQIGVVYFDNDYKFDILLFRSLLFEKYKSLINDYNTTASMIDIDEEKFNVLYKDCCSRLFIVRCKDSFQFMVALRGMPEFIRNTSGSIKPNEKEPLEIRLIMIDSISAFYWVDQCGEGLNVKPKLIWLDPIRRLLTEFSMMIIATKQTIFSPVSSNNNNIVYNQQQDHDHPTTVNQNVIQHKEFLGLEWTKLVKYRKIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q4Q6</accession>
    <name>A0A8J4Q4Q6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004789</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000176">
        <property type="protein sequence ID" value="KAF2073901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q4Q6"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="100" mass="11535" checksum="999AFADAC60CBF38" modified="2022-05-25" version="1">MLSRDLFKSSMPIGLLPLDAYEKDGNLIMIMEIIVERPCDRQNFIFGQRWCGDLKKVIQFKNQVGVPKVNLTDGILTITIPKYESNLILKVDQELHPKFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q509</accession>
    <name>A0A8J4Q509_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_004635</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000167">
        <property type="protein sequence ID" value="KAF2074066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q509"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2074066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="99" mass="11659" checksum="EB01873FBAC48988" modified="2022-05-25" version="1">MRPHFQPNEIKSLSFDKFTTCVQMNHDICSHDYRLRFTENTINCLRECVLVCLETIDPSRLNCFIFPDHSVDEMKAASLPILQEYMTLKNIEPKLANYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q587</accession>
    <name>A0A8J4Q587_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Xylose isomerase-like TIM barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_000144</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AP endonuclease 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000003">
        <property type="protein sequence ID" value="KAF2078519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q587"/>
    <dbReference type="OrthoDB" id="7663182at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00019">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.150:FF:000001">
        <property type="entry name" value="Probable endonuclease 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00152">
        <property type="entry name" value="Nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018246">
        <property type="entry name" value="AP_endonuc_F2_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00587">
        <property type="entry name" value="nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002199">
        <property type="entry name" value="PRK01060.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445:SF0">
        <property type="entry name" value="APURINIC-APYRIMIDINIC ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445">
        <property type="entry name" value="ENDONUCLEASE IV ENDODEOXYRIBONUCLEASE IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="Xylose isomerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00729">
        <property type="entry name" value="AP_NUCLEASE_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00730">
        <property type="entry name" value="AP_NUCLEASE_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00731">
        <property type="entry name" value="AP_NUCLEASE_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51432">
        <property type="entry name" value="AP_NUCLEASE_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Xylose isomerase-like TIM barrel" evidence="9">
        <location>
            <begin position="230"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="147"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="157"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2078519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="493" mass="55464" checksum="C65F61F773A55B5B" modified="2022-05-25" version="1">MFASVSTLKSVLNIKSLSSLRFYSNTIKITNNNSSIISSRITSKRNMSKRKNNDVVEIVDNDDTIDLDDIDEHDCDSHHEDDTEDELSIPTLQKEVESNKKLNSIVNNSTTKVTATAAAKKPAALKQTVISSFFTKVDQPADVHTDKEYLDSRAKKTTTTTPTTTTASTTPPKKKTKKSKEVTILVDYDKNEKYVPELQYSASKKEKMDGIKIGAHMSIKGGIPSLIKSAIDKGFKAIAFFVATPRSWRGPKINAEDAKSFKEECTKHNFSPKYMMPHGSYFLNLGSPEADKLEKSRNLFLAEMKCCEELGITLYNFHPGSHLNMISEEESIKVIAESINYAHKHTSTVTAVIECTAGQGTNLGYKFEHLRDIIALVDDKTRVGVCLDTCHMFAAGYNIDSYLGCQKVFQEFDRIVGFSYLRGIHLNDSKAKCGEKLDRHENIDKGHIGSPCFRYMVNDNRFFHIPMILETEGSHIEEVKQLYSYIKEDNQEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q715</accession>
    <name>A0A8J4Q715_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_003260</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000100">
        <property type="protein sequence ID" value="KAF2075431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q715"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2075431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="562" mass="65412" checksum="CE8F7AB2E9D72E93" modified="2022-05-25" version="1">MNKYIIQRIFNEALAVFGGTRKSIPMETIKQFLVKLILVSKHFQMYILPILHYPLVDIKSMHSFDLAAYLIKHGVRIPLSIKSDKFPLDINSPNIPIIAPYVYKYLINQQAITNQNVAVHGHFKNLRKINIEYHHSDWFHESLTHFTKEEEGEPTFARLNKMTITTYFRCLEVYRPIKALYWDNITAFGLYSATLDLDLILEFKNLKTLVFEGNSRLELLLPLVNLHPKIENLSLDVYQPLGKNILSMIEANRSVTSLVIRGQFTSLEGMIKVLNNNKTLRHLDWIDRISSNTGYQDSNETVSLFIENKTLESFRYLGFHFDLLEKWITPSNLKTLKLYKLFDNMFTFHPMLEHFFINYSSNQQLLEKKKHVWIGAQLPHLRRLELNSMGRDSRRIGNFWKDFIPGTLFSFKLDNLSILNVEMSLIEFTFLFNALPELGVSHLSVTNPGITRYELDRQPLITAIKKYVGQLISLNISSFYVDYEYKENDILFAVLESCSSKFNSFIYDQVKILSDQEEKHLLDIITEKFHITNLVLGYNAVMPMALRNKLASKGVMTGKIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4Q976</accession>
    <name>A0A8J4Q976_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_001573</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000038">
        <property type="protein sequence ID" value="KAF2077126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4Q976"/>
    <dbReference type="OrthoDB" id="6375174at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17663">
        <property type="entry name" value="PFA-DSP_Oca6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000084">
        <property type="entry name" value="Tyrosine phospatase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004861">
        <property type="entry name" value="Siw14-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF14">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE OCA6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03162">
        <property type="entry name" value="Y_phosphatase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="400"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="400"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2077126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="448" mass="49130" checksum="EE40673CAE49DF74" modified="2022-05-25" version="1">MIKSTDPLIPPFRFAIVEEGLYRGSYPTDKNFRFLKRLKLKTIVSLTPKPPMKSFYTFCERQNTNVKHFTVSKFKDDVTLSASQVVQLLELMIDPSQLPMYVHCLDGANVTGTIFMCLRKLQNWNLSVIFTEFTRFTRGGTIASAEAEFVETFKAEIDVQPSIPNWLWQGIRMTKHPTLKLRLLSNSSQNTPFSSNFIQDEYEIIDSANDSIDNSSNSIGNSGGGNSNGGSTSVSRKSSNATSLLPSFMAAANALSISTPSILTTINSSNISATTTTATPSPTIDSNTLNLISQSLNIDSNQYQPQQIILPQQQQQPTFVSSPSPSSLSTSTTIVQNQTLSSLSNSQLITQSPRVITQSGGGKEKDKEKDKKKKVDEIKLLFDSGKFLVQKESRFLEALSLERSSSPASPSSSSPSLPQNVATPTTTTTTSLQQSPLVMSSTELFNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UPY9</accession>
    <name>A0A8J4UPY9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADPH-dependent FMN reductase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008768</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000574">
        <property type="protein sequence ID" value="KAF2069916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UPY9"/>
    <dbReference type="OrthoDB" id="17788at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050712">
        <property type="entry name" value="NAD(P)H-dep_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30543">
        <property type="entry name" value="CHROMATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30543:SF21">
        <property type="entry name" value="NAD(P)H-DEPENDENT FMN REDUCTASE LOT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADPH-dependent FMN reductase-like" evidence="1">
        <location>
            <begin position="6"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="186" mass="20982" checksum="B6183775DEF8C120" modified="2022-05-25" version="1">MTKLVQIIISSVRTSRVGDQVAAYVKNVMQASSPYRFEIVDLKDYQLPLLSDEPNIPAYKNYTQKHTIAWSEKISQADSYVFVTPEYNGSIPASLKNAIDYLYQEWAGKPTVVVSYANKGGSFAAEHLKFITSRMGMKPVNTTPAILTTPDRRDEQNRILNPEVAFESHRESIEKATQELVNSFSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4URI4</accession>
    <name>A0A8J4URI4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006403</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000294">
        <property type="protein sequence ID" value="KAF2072271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4URI4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="120" mass="12647" checksum="5F3AC6583CAE143A" modified="2022-05-25" version="1" fragment="single">LGVPEAYSTILIEGGRLAGLALINKNTSMPIYSLLKPMNVLVRMKLFSNSKAKLKTSDDPPRIPPATTSPAADTAPATATDNPSVTTTPAIGSTATSTTTTSKTHTSSNFLPVNKIVKYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UU76</accession>
    <name>A0A8J4UU76_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_010143</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000943">
        <property type="protein sequence ID" value="KAF2068533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UU76"/>
    <dbReference type="OrthoDB" id="5065185at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.10">
        <property type="entry name" value="Pesticidal crystal protein, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036716">
        <property type="entry name" value="Pest_crys_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35598">
        <property type="entry name" value="ENDOTOXIN_N DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35598:SF3">
        <property type="entry name" value="N-TERMINAL DELTA ENDOTOXIN DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56849">
        <property type="entry name" value="delta-Endotoxin (insectocide), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2068533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="89" mass="9653" checksum="1EB4CAFC81B57F44" modified="2022-05-25" version="1" fragment="single">MEDKSKGIISTKSSEDDFMEATGSILSGAIGSIPVVGSMLSGIFGAFWPGPKYLTVEDFQKEIKKLKEEMINMMDSKIDAALDTVFNAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UUK3</accession>
    <name>A0A8J4UUK3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Core Histone H2A/H2B/H3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_003114</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the histone H3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000094">
        <property type="protein sequence ID" value="KAF2075571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UUK3"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45810:SF1">
        <property type="entry name" value="HISTONE H3-LIKE CENTROMERIC PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45810">
        <property type="entry name" value="HISTONE H3.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="3">
        <location>
            <begin position="348"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="53"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="80"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="116"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="182"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="210"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="265"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="277"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="295"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="305"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2075571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="443" mass="47915" checksum="06CED6C80EF97275" modified="2022-05-25" version="1">MTNRGGGSKKNDTFQMSPTIEPDTEDSSDSTGQRANRLMHAFKSIKGGGTGGESSARTRSVSLQYGDYSTSGSETNISTDDQEANVDTDEYEDDDDPIIKRINNTIKGTYNPPPKTTTTTTTTTSIPPSKKPSSKKEAISTPQQRPITTYSPTSPPTPPSPTTSSTPSSEYSNEEELTPKLSSGRSSQQSTQSLPRSPPPKQPAAKTVIKKPPITSISKPISKTPTTSKKPAAATITKIIPTTPVTPTTSITKKSAATRTIPKSPAKPTPTTPKPKPSISKPKPSPTAKKTAPSARPPSTPSTPTPQQRTPTTTPRKGRITTPRRSPAHGGGSQSPSTPGSQKKRRRSRIAQEIRRYRRTTKMLIPRVPMLRLIRELMAEHRDGLRITAEAASALHEISELYITRIMEDANICATHAKRVTIGTKDIHLTNRLRLSDVTTYPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UUZ9</accession>
    <name>A0A8J4UUZ9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Guanine nucleotide-binding protein-like 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_002610</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Possible regulatory or functional link with the histocompatibility cluster.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000073">
        <property type="protein sequence ID" value="KAF2076096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UUZ9"/>
    <dbReference type="OrthoDB" id="61815at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01857">
        <property type="entry name" value="HSR1_MMR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043358">
        <property type="entry name" value="GNL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45709:SF3">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45709">
        <property type="entry name" value="LARGE SUBUNIT GTPASE 1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="7">
        <location>
            <begin position="257"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="49"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="402"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="631"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="403"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="640"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="660"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="681"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="697"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="720"/>
            <end position="739"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2076096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="739" mass="85344" checksum="20F1B1C4BDC0436E" modified="2022-05-25" version="1">MARSKPFSGKQKKDQLKAKKERKRIEATLLDENIDISLGEKRKLAKQILSEKNKEKLQNKKKINNDDDNDDDDIDDKNDDEIIESENNSDNESNGEYSDTDSVDDNKNSNSKTTAPTTGSSSTLSQINQRMKDFDLSSKDSKINRLVTIFDRESREEIEYRKEQSKLPLNTSLRDKPWLIMKQFDYQDQQFYDANNIEQQREQLYIDIPKRPEWNFQMSAQKLKEQERKSFAAWLENIYNKYDKSRLNYFEHNLEVWRQLWRVCEKSDVILLVTDARYPLFHFPPALYHYIHSELKKPMILVLNKVDLVNSKIISAWINYFSIHFPLLKIITFSSFKSNLDDDTTDGTSVAIDKKRKFRKGRKRYDLSLGKQHLINTVLSLNIEKDNSSNSSNNNNQYYQEQDQIDDDSDNSDDSDDDSSNDDDEYQQHQDDDDSDQDDDNIYRKENPLKDMITVGMVGHPNVGKSSLINGLMGKKVVSTSRTPGHTKHFQTIFLTKDIQLCDCPGLVFPALDRPKSLQILCGLFPIAQVREPYSAIQFLCERTPLEVIYALKNPYPDEPWSAYSICEAFALKRGYLIAKSGRPDPHRAGLEILKECVDGNIVISWPPPDFTIEDFQEIGIYQQSQKHQLYNNTKNNDGDSSDGDNDQDIYSDSDEFFMNHSNGSNSNNSNNNNNNNNNHHNQRKPKKTGKSKTNTKETTSTTSTTSGGGKKKLSSKALEYQERLMEKQNKGGNRRQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVL1</accession>
    <name>A0A8J4UVL1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_009904</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endomembrane system</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000848">
        <property type="protein sequence ID" value="KAF2068775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVL1"/>
    <dbReference type="OrthoDB" id="1877176at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030119">
        <property type="term" value="C:AP-type membrane coat adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09256">
        <property type="entry name" value="AP_MuD_MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039591">
        <property type="entry name" value="AP5M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16082">
        <property type="entry name" value="AP-5 COMPLEX SUBUNIT MU-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16082:SF2">
        <property type="entry name" value="AP-5 COMPLEX SUBUNIT MU-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="6">
        <location>
            <begin position="234"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2068775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="570" mass="64135" checksum="98F17C53F7E528A0" modified="2022-05-25" version="1">MCTLRGLWILKEINSNQSDIIFSKRINTVEVRVRAISGTNYTPIPNDSDLTNIFNHEILNSDQYNQLKQQQQQQQQLKSSTSILNNNININSNTSNNLPPVSIHSSTPNTHIVSLNQDKLWPFVYIKKKSFYYLTIPVIEEFLIYNKKPSLIDLPAITASLNFLEDISLFCNSYLVKPGPYPELQIFLANIIPFGQPTDTNFNNIKSMIKVGFPQVETINQKRPVWKPYLHKGKQQLDFTITETVSTILYDNPNIQDVFKVSGALYCRADLEGMPEVSFYFNPLESPVQQQQQQQGGSTGNYANITSPTTTPTITHLSIDPSVQATSDININNKISFNPPLDYFKLLGYGVSGVSSIPLRGFYQMKETSINSVKILIQLKLNKDMNNSFEHCLVKIPFKNRGTIVFVNASPTTGTVHIDPTLKALVWNIGTKFTGRNLEVALPAEITFSTNHPPQAPTLIMSGSSGTQTLTGFPNQSFAIIDQAPQDEFLANDPFCVGPNSFIRIYFKILDCTTSTLNIDPKKVVIYPANKFKLNIERAVISNEYFIWNSLGNSKKAYQPDLHLNSKLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UVX6</accession>
    <name>A0A8J4UVX6_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009514</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000729">
        <property type="protein sequence ID" value="KAF2069163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UVX6"/>
    <dbReference type="OrthoDB" id="10663817at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035203340" evidence="2">
        <location>
            <begin position="24"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="780"/>
            <end position="804"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="848" mass="91386" checksum="9AD399422365AEAF" modified="2022-05-25" version="1">MISKSSSILSLFIFFLFISGHLCQVIDTEVTINDKVSLNGNTLTIAAGGSLVLGPDVTLDLTGPCSISSPVLIELVFKSINIIPDPNTNTQVLPTLQLTNIKLKITGPTTVQDLKIMMDGNSQFISTSTTEFKYSLFASEGSAFVGGTLSIPTGSFTMSSLTTKTLKLESKIDNKSQFITKLILLAINIQDLLPVTGGTLTAPASQYYLSNGLKTLGIYPSSTSLDLSGVYLRISGVLNLGGATLFISATIIEVRDSPVEVQNTRIIVNQPCVINAARSASMYPLGSLILDSQSTLTLSQANSLKLSLIVKSDYYMEVGEVTSPYPSSDPIIYTTSGNNPITCGNYKGGSGTGFVSYQLSNKKPKLRGYAYCNSRVISVRSNLDSETSSDEIVDLCPEGEYRCFSAEGACTPFETVCDTACGTTAPQLQSQSGRLSRFEVKSALVNRQNSTHIEKARESLFSNIIDGICWDGTCSQVYPTCPPFPECGVTTPKRCAGYCVAANAQCDDNACVTRCADGCYNYVRSCDQFNGCGLDEIECPDRSCVSDVTQCPPTPPAGCGTDCWIPQSKKVLTSVTNAFVSSYSLIPSWYYDPLTLLGQPSVQAEIPSASFTPAIPTVPYSLVKIRNIPDSVVRGLSLPTVAGFSYYTDVHSSVFSITLDNQASTTFTDKVKLLFPKPSYITATNIDDYCLGYIKTVGSTQTWECTPDPLTISVDGLIVGKTDHFTNFGVLTKYRPTSTSTSATSSTTNFQSFTTTSQQTTYYGDEIDNPPKQSSNTNRIVIIAASIAGGVIAVVIVAAIYVNFKQKHVSLRRWSVRRSDPNLERHLRALRESNQSIGSNSSSENNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UW97</accession>
    <name>A0A8J4UW97_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">CYY_009307</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000676">
        <property type="protein sequence ID" value="KAF2069373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UW97"/>
    <dbReference type="OrthoDB" id="346907at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF288">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="241"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="276"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="15">
        <location>
            <begin position="335"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="548"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="434"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="835"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="438"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="452"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="485"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="858"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="575"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAF2069373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="927" mass="103467" checksum="616B74F44E0232A1" modified="2022-05-25" version="1">MTKKVGSIHIEVLEGRNLIPMDSDGQSDPYCVIIVGEKKKKTKAVRHTLFPKWENEHYDFNIDKNLGSIVIEVYDWDRFSSDDRMGMVVIPSTQFSDYSLEITKWFTLIPIKSEDPVSGEIKLKIRYDKDNSQPQEKNPLIKAIKDNDVAAIEHLMNKSKVDYGICDSEGTPAIHLAIGTNNIPLITLILKASDLKITIKDSHGNSPLHLFSQKCITTTQSDEVILKLIDKGCSVADENALGEIPLHKACLSNFHKTSTIDTLLSKGSNINHQTKTGDTPLHYAIKMNRSDVVLFLLQKGANFNIEGGKPSKKPIDLARELDHTSIVNKLEKVLEISDWLNNLQLESLIPIFIQNEIYMDVITDIDESTLDSLKITVSGQRAKLLRTIKKMKSELNTSFSSLSGSFSSSTSNLKTSYNQSAEDLKYLVNNSPTKVEINSNNSDNSNNNNSSATTIENTGSNSPPIVNDSTPIVNNNNNNHKEENSNNNKQDIDNNNNNKQDENKDNSIAEQPPTTTTTNVINLNVSELSQLKHIHLDSDSWVIDESTLKYSVLLGTGASGKVYKGFYKGIEVAIKVLKSFTDKKDIAEFKKEFQIVSSLKAPGVVNFYGAGIKDKLCIVMEYCSRGSLYHILKDETVQFDWNNFFHLGLQAISSLDSLHCWTPQVLHRDLKSLNLLVTENWTVKICDFGLSRFDTGSNLETLGKLRGTYAYVAPEVYFGNKYTTKSDVYSMGMILWEMTYRCINGVHQFPYQEYPHLKFDYQILISSAKKDVRPTIPPNTPKSLADLISLTLLKDGASRPTTSEFYDALLKIHQEYLANTQEWDSKRIIPESQLPKPLVPATDTPGSSSPALLADVDNSSPRSFTNNNTPPTPSPSSSSNDFTQPIIPTTTSTTDSIKSPVSLLKEQITRTRSSSSPIDPKSLLKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWI4</accession>
    <name>A0A8J4UWI4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000634">
        <property type="protein sequence ID" value="KAF2069598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWI4"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="64"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="201" mass="22166" checksum="D1A91F07BC7B68DD" modified="2022-05-25" version="1">MTLQNSTLSNQASLNSSTDEQSSSSSSATSTIGAPSTQIQSPTQIQQQQLNTTTPTSPSPSSPPTSSSTPSSSILSSPTDSTKTNNTIILKSDLQTSNTKNTKNTNNNNIIIKKPSQRLQCEVYGILRFLACVDPNCKVKDLYKILQDEIEQIYKIKLKILLIKDRNNENVSSNESNISEIFVENVRSYGDTDNEIFIYKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UWZ7</accession>
    <name>A0A8J4UWZ7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Right handed beta helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_008373</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000510">
        <property type="protein sequence ID" value="KAF2070305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UWZ7"/>
    <dbReference type="OrthoDB" id="24297at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31318">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31318:SF1">
        <property type="entry name" value="POLYMORPHIC MEMBRANE PROTEIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035243717" description="Right handed beta helix domain-containing protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="518"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="445"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="548"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="445"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="478"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2070305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="567" mass="60757" checksum="50DEC8C673D3BB01" modified="2022-05-25" version="1">MFYRKLLFVCLFLTSITYINCDGITLYVNSVASNKDPNCGESIDLACTSIQEALSSYSLSTKGENSTSLTLELLDGTYDVSTNAPLGDIYLEKLNVVSYSNSNKNLFLTGSKDEAPFFSYLQNSPIGQSLLIANITFINSYKIILSNSNVDVVISDCVFRDVSNQTYSSMISLFSNQNPSASISNSLFTNISLNVGTITKFGNYTLTIDNNKVLNVIANYIFFFDTSSVDIENLVINNGSTTFSPIGAGKSSFSLKDGSFSNNFGGSSGVLMFFLQDKPLNAVIQDSSFTDNLTPFTGGAIYFSTNNGLSVVNNCNFSSNLVYDKQGVGGAIYVNSPVAFNNCIFNNNTANNGGAMYINKSNATLTSSTLFDNKADQGGAIYTYASKVSVDSDSLANNIANQGGEVFCYGSSINITLANNNPPPSGYYCPFKDCTFNAPSSFKCTTPSPSSTSGSSGSVTPSNQTSSENAPPLPSPTPNSGSNDKNQSIPNSSTDAAKNDGSPTSPKGKDKDSDRTKIIIATICSIGGVCLVVAVIYVSVRYHRKRHHHSHHGEYNQHSPLMKHGHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZN0</accession>
    <name>A0A8J4UZN0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_004020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000135">
        <property type="protein sequence ID" value="KAF2074670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZN0"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="710"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="833"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="79"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="115"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="157"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="278"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="355"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="400"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="466"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="476"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="513"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="549"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="587"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="598"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="624"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="651"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="722"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="846"/>
            <end position="858"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="858" mass="95898" checksum="F5BFF62286E05388" modified="2022-05-25" version="1">MKGGKETTTKLNITSSSNTNTKSPNKIKINKNNNNKTTTTTNTTNTATITTPTPTKTEPTPQPSISTLPVVPNTPPTTTPTSTTTTVTSTPSKSSNHNHKQHKNKKEGIPQNQETQKASSSQSPSQSSTKTTTTSTTTPTISRNLQDKNSVSVTVDKTSPTSNTTTKPSSNKNKTQSSPTQSSTPSTTTSTTSTTSTPTRESQPRKQRQPKTREKKENTNNNSETTKDNVAQKSKQEKQENTSVKNKDTPLKEKEKEKETKVTPQQKPTHSEKQSPKQQQQQQQQQQQQQQQQQQQQQQQQPKEGGERNVKRQSRGNNRNEGKVEKKEQQQPKEGGDKNVKKQQDEQPKVVEPKTTTTTTTTTTTTSLSPSSTSTGSSSSTTKSTDSDPKKNKRSRGKKTNNSGSSHTGSTSQTTETSTLQTPKTQSLQTSSSSATTPTSPTLSTPSTPITTTATTPVVNKETKTQAKPKQIKSNTSVVQKQQQQQQQAPATTTPTATTKSQQQAQPISPKQNTLPITTTATTARTTPTSTTNNKSNNNNHKRQQHNTSLSTPNSTQKQPQQSSIKKEKENVENNKTIHQKKHTQTKPKITNNDNDNGVDKKNRETKNNNNNNSNNKKQEKEVSTVSIDKTVQQPNNININEQPTNKMEPTTSASITTTTTTNTHPESQQQSQQQQQPHPFIFFPYYPPPPFYSQQQQQQIPVAAFNNLNISPRKSNEPFMNGNSNSSNNNSNTLDEKQVLKQQSAQQQQYQQPIYPHPYIIPYEYYQNFYQYDPNLQQQQQQTHQQSHQQPQQIHPQFMSFGSFPNQGYPPFIRGYQPQQQHFEGYVPFEGGQQHHQQQHHHHQQQQQQQQQSQLPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZU7</accession>
    <name>A0A8J4UZU7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosome assembly factor mrt4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_003820</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Component of the ribosome assembly machinery. Nuclear paralog of the ribosomal protein P0, it binds pre-60S subunits at an early stage of assembly in the nucleolus, and is replaced by P0 in cytoplasmic pre-60S subunits and mature 80S ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 6">Associates with the pre-60S ribosomal particle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000125">
        <property type="protein sequence ID" value="KAF2074865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZU7"/>
    <dbReference type="OrthoDB" id="10262308at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05796">
        <property type="entry name" value="Ribosomal_P0_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1730:FF:000005">
        <property type="entry name" value="Ribosome assembly factor mrt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000003">
        <property type="entry name" value="Ribosome assembly factor mrt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033867">
        <property type="entry name" value="Mrt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051742">
        <property type="entry name" value="Ribosome_Assembly_uL10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841:SF1">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 MRTO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="7">
        <location>
            <begin position="126"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2074865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="216" mass="24947" checksum="008810F3323C4B00" modified="2022-05-25" version="1">MVKSKRNQVVNLTKVAKNPGEKKKKLVANIRESVDKYNYIYLFTYENMRNNLLKKVRTEWGTSKFLFGKNKVLSIGLGKSDSDELKTNLHLVSQQLEGECGLFFTNEPIEKVMEFFKNYTENDYPRAGFVPSETITLPEGPINMTHSMETYLRNLGLPTVLKNGVITLERDYNLCEEGEAITPEQSRLLQLFEHKISEFKLNIKGYYHNNVYTTTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4UZY3</accession>
    <name>A0A8J4UZY3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005028</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000190">
        <property type="protein sequence ID" value="KAF2073678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4UZY3"/>
    <dbReference type="OrthoDB" id="19222at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01610">
        <property type="entry name" value="PAP2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="302"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="333"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="372"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="411"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="2">
        <location>
            <begin position="357"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="429" mass="48511" checksum="1907893C3EA70A98" modified="2022-05-25" version="1">MEPFDPINSKIYRTINRSIGSKSNNPFNTLEKAVQNNLIVIDSSNSISSNPTIRNSNSSNNAASTIPLTSPLLNNVFEGVPITFDNNQNAISTPPPTTTPATPATPFWLGECLRWGLFIDFLIIILSIINFSISYTPSLPSVTLYWPLSSLSMVHGGLVIFNGLLSTWKGYSVDGKLFYAFHHFISMIFFFCSTVCFEDIQYFAAFAIYLTMRIVKRAKDDGYINKDQWVTFYISYAYAEAFVNLFVLCYYKNYKFTMIDVIGCVALIDSMITCRKENKTNKGITNYLFPVDLLANNTVQNILSVLDVLTLWSMLIYIGGLEGANYEFANIQTYQGLIIFATVEFLIKPLFKNFKRPFTYKFSNHSSFPSTHTVIAMLLVTQITGCLNRLLFVFAVGFYRVYTRAHTVEDVLAGVSCGFIFKTLWSLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V001</accession>
    <name>A0A8J4V001_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lipid-binding serum glycoprotein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000121">
        <property type="protein sequence ID" value="KAF2074955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V001"/>
    <dbReference type="OrthoDB" id="9938407at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.10">
        <property type="entry name" value="Bactericidal permeability-increasing protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.20.10">
        <property type="entry name" value="Bactericidal permeability-increasing protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017943">
        <property type="entry name" value="Bactericidal_perm-incr_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032942">
        <property type="entry name" value="BPI/LBP/Plunc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10504">
        <property type="entry name" value="BACTERICIDAL PERMEABILITY-INCREASING BPI PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10504:SF142">
        <property type="entry name" value="UPF0522 PROTEIN A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55394">
        <property type="entry name" value="Bactericidal permeability-increasing protein, BPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035268503" description="Lipid-binding serum glycoprotein C-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="495" mass="53529" checksum="AF2653DDEB17F27C" modified="2022-05-25" version="1">MNRSSHIFVLAIVAITLISYVQCQYPAAGVYVGINENFLAQAGQDLSNYAQTKLENYPFQDISGSSSQVKYTFHDFKLSINFADFYYAQASSSQYAVGWENVQYQLQWNYHICAKTVFNPCEDGTITVFTTSANNPVNVEATLNVNTEQDPISVSASATTMNFNSGAIQVNVHCSNSICVIPVGDIANEVAQNFVTDACNGITTAINDMAPTIDSLFNPIRVLPFTFGNKGYQYLLNAEGEIVVSSGSGYTPAITAALQGGIIVKTPSGSYYPSQNPTYVPESSQLEGFSQDYMLSFSGYFFESLFDAAFSSVMPMTIIPSEVPAASPVTLNTSDPFFTGVAPGLSQYPGLGIQVSIHSPVSPVATIDANGVTLNGTTLLGDFIIMDESSPLTAFTVQFDIDGQINTEVHMPSTSEVSLNTTIVSSNPNATIISSAVGPVDPTGFVQLLQMFLSVMKLPSFTYNVPSAYNLEYIDITYQSQLVQILVDIEKNLYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V122</accession>
    <name>A0A8J4V122_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MRH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008694</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000561">
        <property type="protein sequence ID" value="KAF2069992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V122"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035144874" description="MRH domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="197" mass="21513" checksum="D10604A72689813C" modified="2022-05-25" version="1">MKVFLTVFVCVFVLGCALADLEFVQTTRYAAIDSSCSNSPLSINAQLSGATIYESTYTCTPDGTTVEINANETLAALTTGQCLTFEGYYARFDCVAQPIKLKNYLLLLEYSDCWVSPDNLVKYSLEATNQCISNPSNPSSYAYMSCSFTNSVEYIFDASLPANSSDSYCPGALIGKRVNTWTENQCVGGFAQVYDCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1J2</accession>
    <name>A0A8J4V1J2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PiggyBac transposable element-derived protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_010486</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001145">
        <property type="protein sequence ID" value="KAF2068189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1J2"/>
    <dbReference type="OrthoDB" id="9985837at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029526">
        <property type="entry name" value="PGBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34500:SF1">
        <property type="entry name" value="DDE TNP4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34500">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13843">
        <property type="entry name" value="DDE_Tnp_1_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="PiggyBac transposable element-derived protein" evidence="3">
        <location>
            <begin position="325"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2068189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="500" mass="58096" checksum="D810962BAAC09298" modified="2022-05-25" version="1">MYHITNITINTNSINISQASQSYAIECLFTGFILLCIYIFVLAREKNKKKRNLSDNTSAGTQQPNEVDPKDVEVINDPNSSFSLYSSQPKLKKQKNEKNSKDVKKVIEITIQHVLQHNHTLHSQIRINDDAFTKWLYILSKEVTTSDLNIYLGYIRYGPTNTPYLLFEKIKIKNLATSFHFDQLDPDVGVEMQAELIIKDILDGKIVNYESVDKDYIDISKKPYYYPSATNEEGSEEEETIEEEDDEKDNEGDDGNEGEYEEKQIPKFKNQPLTFNNEQINNISFMKKNPFPIKNLKQGPTFNVPPRSAISEIVQLVFEKYFNIFIEILHQETNRYLEERRVQASIKCINPPWFTKYESFSLQDMKDYIGIFIFSGIQRVTNYKEFWEKPNSPFMYSSYHNVVASKMSFDKFKAIHRSLHFEGFGTEGSPTNFDILYSFANNLIKDLYLPSYDMSFDDDLFKWLGKGGQKIIPGKADGVGNVLWKLVDSSTYIYHFEMIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V1T0</accession>
    <name>A0A8J4V1T0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_007920</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000449">
        <property type="protein sequence ID" value="KAF2070757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V1T0"/>
    <dbReference type="OrthoDB" id="185175at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051707">
        <property type="entry name" value="PI-Interact_SigTrans_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14336:SF8">
        <property type="entry name" value="PROTEIN OPY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14336">
        <property type="entry name" value="TANDEM PH DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="35"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="206"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2070757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="261" mass="29586" checksum="2334B3398A3558FE" modified="2022-05-25" version="1">MKKSSSQLNIGGASSNGEGSSIIAMDVSYDTLTEQIKKYGYLKKLGGKGISKNWRKRFFILTNSGILYYFKHRTSSEAVGAVDLAEYTKIYKDKTKKRNYFLLVNENKPEQRVFHLISESEQEMGSWISDITEFFEDDVSDLKNQLNMLSIQKREKIDTLKRNVIKSKRVEEYELDKVQLVFKGERLSLLFKNSPTTGSVNTNGNTLESTLLHSSSTPLDNNNSDNNITNNNGNDENDDLNIINYDDDVGGGDEDDEDDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V234</accession>
    <name>A0A8J4V234_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_000827</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000017">
        <property type="protein sequence ID" value="KAF2077865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V234"/>
    <dbReference type="OrthoDB" id="14955at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45911">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45911:SF4">
        <property type="entry name" value="MULTIPLE C2 AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="16"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2077865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="161" mass="18830" checksum="B3AA2D1D1781F0D8" modified="2022-05-25" version="1">MTTEEEYTGPFAHLAPKDRLKKKIKPTKNYDLHEVFRITVLNGIVKKSTDSNGKSDPYVRVSKKGKFGSKKTLFTTSVCKKTLTPSWNEWDNFILSPKNKELIFELWDQDTFTKDDLIGTYHFLPSLPYDMFSNWEFKLNDEVTATIQFIITDYYHPDSDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V3V5</accession>
    <name>A0A8J4V3V5_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000237">
        <property type="protein sequence ID" value="KAF2072947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V3V5"/>
    <dbReference type="OrthoDB" id="31141at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.980.20">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="584" mass="66456" checksum="CA1816C306B260D3" modified="2022-05-25" version="1">MTLPEILENKNIIPISRINGSPITPRPNNFAKPPHFLIYQPNNSLNNSPSEDYTSPLLLQNNQIDWPSQKPSPQQSSPILLSTLKNSLNISNAPNDIQQQQQQSLSNLSCSSSSSSFKYNNDDLIILPTIPSSPNINRSNSPMSCNESDLSKSPSKMLNNSFNPLISSSSSSNGIPTLSSSPTSSPTFKSLCNEQGCMICANTTPYCLSGKFPSWSAILWVCLYSLHKLHPEKDFVNLREDVYPSILKHWSKICGNRETTSNWKKQIQDSLSHSRKLFESGVDILGQNGYWRLKKDLNPWNIETSMHQVNQYEANNEKSNDSNEEEKPFAKKKKVINNNNSNSNNINNINNINSSVSNNNNLHNNKNNVKNQPFYNNSFILDSNNNNQSNHISPDFHINSAFNNIKPNNSTYNLNNNNNNSTYNNNNVININQNSDRLFNLNYVDKVRELQIHLSNNGNMNNRSLLLLEFKKSLEQSMLNANEAMKMVSNLLVNLDENNFNQNFNYNNLINNNNNNINSNNNETIGFQYYSYNPSTNTNQSLNNKKKKRNNEIIETTPSFEFSMYSHKNENNNNGIMVNNEWRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4Q1</accession>
    <name>A0A8J4V4Q1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">2-dehydropantoate 2-reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_004906</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ketopantoate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000183">
        <property type="protein sequence ID" value="KAF2073772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4Q1"/>
    <dbReference type="OrthoDB" id="3609at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008677">
        <property type="term" value="F:2-dehydropantoate 2-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000017">
        <property type="entry name" value="2-dehydropantoate 2-reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000307">
        <property type="entry name" value="2-dehydropantoate 2-reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003710">
        <property type="entry name" value="ApbA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051402">
        <property type="entry name" value="KPR-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013332">
        <property type="entry name" value="KPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00745">
        <property type="entry name" value="apbA_panE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708:SF26">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708">
        <property type="entry name" value="PROBABLE 2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02558">
        <property type="entry name" value="ApbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketopantoate reductase N-terminal" evidence="4">
        <location>
            <begin position="5"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Ketopantoate reductase C-terminal" evidence="5">
        <location>
            <begin position="184"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2073772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="316" mass="34858" checksum="55F6418B19F0588E" modified="2022-05-25" version="1">MNTKILVLGAGAIGGYFGGRLVEAGQDVTFLVRLNRKKQIEGNGGIVVINSVNGNYTFKPTLITKEEGDGNVEYDVVLLSSKSYHLQASIEDLKPFIGKNTLVIPLLNGIQHISILEQAFGRERVLGGLCVVETTLNDKGEIHQPSELNQLIFGQLDGGTTEKLNQLAQAFGSTKAKFTLSESITQDMWNKYLMITTMSSITTLMRTPLGTIRDSVGGLEFIESVFKETESIMRANGAPLSDGIVDRYMTLVKTISSQFKTSMQRDMEKGLEIEGDHIQGYILQLAKQHNISAPLLRCAYQNLCVYNEMLKARSNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V4U1</accession>
    <name>A0A8J4V4U1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutamine-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="8">6.3.5.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">NAD(+) synthase [glutamine-hydrolyzing]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_001104</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>deamido-NAD(+) + L-glutamine + ATP + H2O = L-glutamate + AMP + diphosphate + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24384"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">In the C-terminal section; belongs to the NAD synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.1" evidence="8"/>
    <dbReference type="EMBL" id="AJWJ01000025">
        <property type="protein sequence ID" value="KAF2077563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V4U1"/>
    <dbReference type="OrthoDB" id="2020662at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00334"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07570">
        <property type="entry name" value="GAT_Gln-NAD-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000036">
        <property type="entry name" value="Glutamine-dependent NAD(+) synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.110.10:FF:000003">
        <property type="entry name" value="Glutamine-dependent NAD(+) synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02090">
        <property type="entry name" value="NadE_glutamine_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014445">
        <property type="entry name" value="Gln-dep_NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00552">
        <property type="entry name" value="nadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090:SF9">
        <property type="entry name" value="GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006630">
        <property type="entry name" value="NADS_GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 8" id="KW-0520">NAD</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="9">
        <location>
            <begin position="4"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2077563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="703" mass="78989" checksum="9FE90EFC3EA8A12A" modified="2022-05-25" version="1">MKTVTLATCNLNQWAMDFKGNLERIIESIKIAKNRGARYRLGPELEIPGYGCEDHFLEQDTMLHCWQSLATILSDSELTNDIIVDVGMPVLHKDVRYNCRLILHNHKILLISPKKAMAMDGNYRESRWFTAWIKSRVVETFYLPKIISDICGQDTCVIGDAIISTLDTAISSETCEELFTPNSPHIQMGLDGVEIFTNGSGSHHQMRKLDTRVDLIKGATSKSGGIYLYSNQQGCDGGRLYFDGSCMIMINGECVAQGSQFSLHDVEVVTATVDLEDVRSVRASFQARCAQANLTHEFPRVRCLFLLTHTDLFHPPSRPVSIRYLQPAEEIGFGPACWLWDYLRRSGLNGYFLPLSGGADSAAVAAIVAIMCQLVILDASKGVQQVIRDARRVAGAPEDYVPKDAKEFASRIFRTAYLGSKNSSQETRDRAKRIAADVGAIHKEMTIDDTTTALNLAFAQITTKIPQFRSNGGSPRENLALQNIQARTRMVLSYHLATLLLWEEGRPGSLLVLGSANCDESLRGYMTKYDCSSADINPIGGMSKIDLKSFLSWAAKNRNIPSIFDVLNATPTAELEPITEGYVQSDEADMGMTYEELSIFGKLRKVNRCGPVSMFERLVAEWPDKEPTLIAEKVKKFFYYYAINRHKLTVLTPAYHSEGYSPDDNRFDHRQFLYNPKWEVQFNTIDIIVDKLKQNSCYFENKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V570</accession>
    <name>A0A8J4V570_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">CYY_007084</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. ROCO subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AJWJ01000359">
        <property type="protein sequence ID" value="KAF2071594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10252328at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1390">
        <property type="entry name" value="ROC domain from the Parkinson's disease-associated leucine-rich repeat kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032171">
        <property type="entry name" value="COR-A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057263">
        <property type="entry name" value="COR-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020859">
        <property type="entry name" value="ROC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF113">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PATS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16095">
        <property type="entry name" value="COR-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25497">
        <property type="entry name" value="COR-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08477">
        <property type="entry name" value="Roc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51424">
        <property type="entry name" value="ROC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Myotubularin phosphatase" evidence="17">
        <location>
            <begin position="645"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="domain" description="Roc" evidence="18">
        <location>
            <begin position="1451"/>
            <end position="1642"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="1977"/>
            <end position="2253"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="2576"/>
            <end position="2615"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="2616"/>
            <end position="2655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="319"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="491"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="724"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="795"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="2260"/>
            <end position="2367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="2678"/>
            <end position="2711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="2753"/>
            <end position="2807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="319"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="342"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="374"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="491"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="728"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="2284"/>
            <end position="2297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="2307"/>
            <end position="2323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="2324"/>
            <end position="2334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="2338"/>
            <end position="2347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="2348"/>
            <end position="2359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="2685"/>
            <end position="2696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="2697"/>
            <end position="2711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="2760"/>
            <end position="2804"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="13">
        <location>
            <position position="931"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAF2071594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="2833" mass="319113" checksum="B7C5D273D05E6406" modified="2022-05-25" version="1">MDVLNLSIEFHHPEGTNVTKKIVRIDPATKIGEFSKLLLDKFGRSSEVDPLQYQLLLNTPKSTVPFQVLSDLNKSIANYNIKNNDELVFKKRPKKVHPSNAKLTSKKKPDSIFKTLFSMSTLEMKLGEEKTQPEISEVENQIDDPLVLFKAIEYLTQHFQSAKDVDEILASFQYVGSEIEYTELIKLVNQNEHSDIPQAYRNPKILCSFILNLLHLSFSQTSLTYSLYTTFLKSTNNDIKSSIFYDIPPKSRIILKHIMLFLSSLTCKDPNLLDTISSIIAPFIIGSVEAAPSSTTPPQQQQQLVNNLNNSFKPLAHTTSPLMSSSNVSSSNINISPPPTPSFSSTTQTPPSPSLTSNNNNNNLSANNTIGRSHNRENSKDSSRTLSPNENNICKKVAVDLIQNLPLYLLFSPLKVSQLEGERVLFSSENIYCIDKSNFPPLKASLGEIWVTNYRVLFVNSAKRDSMIPNSSSSTSLSSLLLSPSQSYTSFTSSTSNTPNHSGSYYTPTTTTTTMNHSGGMPPPSTSTSTSPPLGSSSGGTGMTQSVSSLSLAALTNLTKLSTSDNSEIPLSMIYKWKPLKTGTLYESFKIYCKDFRCKVIGFPINSPFLYKFKEILESLKQYSSSPQQTFAFQFNEKNLAHTENFSESLLLKEFNRQGISWDHWRICNVNSNLKVCDSYPSWSVVPKLVNDNIVVTSAYYRSFRFPVLSWNHPNQKSSIIRAAGPEESNSGGNNSHPNQSNSSGSLGYNSGNNVLNSNSINNTSTSNIVGGGFGLNPINTPSSSYKSASFMLSPTQNHSAMSPTSTNTPRIHSSISSSAIPQVCQEDIDFLKTILDIKSSNMLTVFDTGPNAAYASTMIGCQIEFLELANTAKTKERYNKLMHLHLTNPDAQWSETIRFNWLDPLKTLLSAAISIATAIDQGRSVLIQHCGDPDIDAQISSIAQILLDPYYRTIDGFRVLIEKEWICYGHQFAKRSNYDQKFDNFSPIFMQFIFIVWQIWKEFPTSFQFNELYLITILDNIFSSRFGTFLCNNIKERTENNIYSGTKSFWSYVEQHFDRFMNPYSKVTFPLPAIQHLKCQRIYQDTMWNEYFYRYCFKSSLAIEQLEERIRATTPQLSAGVSSSAIPTFEFLDLSGLKLYYLPSDSALYHISNLRELTLAKNNLNHFSVKLSSLINLEKLSLEDNCISNVPYDSMCLISDKLVNLRELNLSTNQFADLPHELARFKNLTSLNIRNNKFVSIPDVIAQLHGLVELDISDCDFSQSSSPTSVPTNLDKLKLLNLNHSRLGSLTKEFGDLKSLVKLYLDFNNLNSVPSSFAQLQCVEELSLNFNQFTEFPEELCSLASLKKLSMEGNQLTFLPNTISQLIYLSDLNLKQNKLESLPASIGQMSNLVSVNLMNNNLTALRPTMGLLTNLTSLKLDGNRIKTPPPEIQVQGLKPMLLYLKDLIKGQEQCYKMKLMIVGQENVGKTTLLKTLREKKKKAALGPNISTDGISIDNWVFSGQFEEKEEENNRTIKKKQDVTLSIWDFAGQEIYYTTHQFFLSERSVYIVAWNLCLAEEESRVEFWLQSISTRAKDAPIIIVGTHLDDVNRNNAKPLKKKMKEKYCTRFPNIKAIKLVSCTSGKGIPSLRETLESVVTSQPTMGESLPRSYMLLENLVKEETKKRVIPTIPWSEFIQLGSICTITDEAELLRATMFLNQLGSLVYFPKEPGLKQFVILDPQWITTMLSSIITTKHSYAKDGILMHKSLKQIWRPPQYPNHLHPHLISLLEKFEISFNVSQDSGFENGMSLIPSLLSNERPAAFATMWPPYDPHVQQFGRIYQFEFIPNGFFSRLMVRILNFARTEVKCYWKNGMLLHHDQEQILLELNHTKKSLTFMVRGMNSATLSRDVIETIQSLLDDSFQLPTQIYIPCVHCLQLKTNHHPYKFPLEVCENAAVKGTGYLKCQGVYNVRTDLLVPDLVMSNFTGAKIPFEELQMDGLIGEGGAALVYRAIWKGQVVAVKRLKTIENDNDPDCPIEINDISLSKAFKEFRRECWIMSDLEHPNIVQLRGLCLDPLCIVTEYLPHGNLYSFLHGDSSNEMGWIFRLKVALDISSGMAFLHSSTPPIIHRDLKSPNILLASLDESSATIAKVVDFGLSGLQHTITNRGVENPLWLAPEILSKTKEYSTQSDVYAFGVILWELLTRKDYFGQYTFMTLIEEKVIAGERPDIPSDCHLLYAQLIQDCWQNNPNERPKFSEIEDRILNIIETMFPDLPLYEPSNHHEKRKSLNSSLNGIRHRKNPSTSSEISIDTHGTSMIDDGVENGGSSSLKRQQSQESNGHTTTTTTTNTATDEMESLKRSKTDDSISSYGIDESSSQRSLAPSKSELELEFRNLDLINNSNSAPSTPKKVLYMNKRNSISVQPFYNEFTKELLPNEGTIQCLQRVGNHVWVGTGSGVMSIWRADNSEKVKSFNAHTKRIVCLYNYMNNQVWSGSVDGSISIWSADNFSLIKTLTGYSPTCFTRVGHTMWVGSIVNCVHVFDLKKKVKYKGKIQLKTGPVECLLRRGENSEVWAALNNQIARIDVNSQRVVQMLNAHDKAIHCMIEVEGTIWSSSSDGMIKIWSIQSGQLLESIEAHKSRIFDLILVGDYVWSGSWDTTIKIWSIKDYRLVSENQGKHSDAISCFVYLNPEETFTSPATLSPNEKDKVKDKDKSTTTTNNNQQQQQQQQIQIQIPKQIWSGSWDSSICIWNLYNGNGHDFYDRTRTNSSMDLKLTPTNSNNNQNHNNHPASSYTPASTSSLLSSIPETSPPLSSSPTHSSTLRGQARRTVSFVFDKFGSSPSSQSSSSSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5E2</accession>
    <name>A0A8J4V5E2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_004273</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000148">
        <property type="protein sequence ID" value="KAF2074417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5E2"/>
    <dbReference type="OrthoDB" id="407410at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070939">
        <property type="term" value="C:Dsl1/NZR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060628">
        <property type="term" value="P:regulation of ER to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1420">
        <property type="entry name" value="Dsl1p vesicle tethering complex, Tip20p subunit, domain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007528">
        <property type="entry name" value="RINT1_Tip20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042042">
        <property type="entry name" value="Tip20p_domB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13520:SF0">
        <property type="entry name" value="RAD50-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13520">
        <property type="entry name" value="RAD50-INTERACTING PROTEIN 1 RINT-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04437">
        <property type="entry name" value="RINT1_TIP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51386">
        <property type="entry name" value="RINT1_TIP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="264"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="333"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="537"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="812"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="57"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="269"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="358"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="818"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="834"/>
            <end position="861"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2074417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1017" mass="118177" checksum="A1E68261A0D7C3F7" modified="2022-05-25" version="1">MSRLAPVNQDHSSPVGSEHHSNSNSNVMAKVEPMNVTTFLDTHFYNESFLSSFTTLKEQLNKEKQVIISLIQKLHEKESIDKLESNSLDAISQLNQLESKRKDLSNELSKQVEKETSVDRLSKYTHLINQLTRIKKYFQTLLIIQMFYSKVKFCIDQSMLSLALKPFINLIELRHNFHTIRIPVMVENEEGRTQSCPHLSKVVDHIVSNLSKHLEKRMKEALNKSMKSINWINFDAKKDLMGVNTNTLDIHDGGSSLNTMMMLIDNDTDSNNNNSSTNTSSSSSSTTTTSTATSNTTPPQTPKKKQQQESIYQEFMKNFTSLVLLQLSTQKYYTPTSTSTPFPSPLVKPNNDSNLHQSSSGISIGGNSSDINNNNNNNNSNTASSPSTATTSNNNNNNNNNNDEGNDRLWAIDIILEPLIKGFKYHFQTARVTNIIEKPEWPIHYIKKVIRQYSPFLIILQRSLERNNIFFVDVHQWFISGLVDTLKYKFRIDIRVFLEKPEIYKGYFYHTMEQIVDFQRFLSEEYSYPNPIEYGNLKNSLNNNNDNNGNIDGSNNNNNNNNNILYNKTPVPFSIFQEKEIFDRWLQLEVNSCDEYFDTLLKSVDRFKPYYRDVFPDIDQLKPTNSSFELINLLRLVTDRYSLLTDTAPQFEFFIKIQMDLLIRYKGELSILIDFPTDHHHFSWTHKQNDHLDEDQLLEYCNIFNSINYIIKILKDWDDELIFIEIYDYMNQKDQDIHYLLSNSSTIKGDENYSAFSAVISDYKDLGKLLIQHLVDSLFNTFSFKIEGYLKRGTFSTSKYNIFGLNRNQHHQNSSVIDNSNKSNTKNTSSSGSNKDDKDNNNSNSEKEQKEKELEDKEKQSNKFNNDEDSQFDVSFEIANALSILRYQLGIIYRYLTPLKLASLWKKLLLRLDSHLFKLLVDYRPKFTFTDGKQFSKDMKTLFLIFHAFSNAPENYMKKIREACILLKMDNHELQLFKEEFNMKSNLDLEEELLASRSLFNLTSNQVQEILKLRINI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5H5</accession>
    <name>A0A8J4V5H5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Pyruvate kinase</fullName>
            <ecNumber evidence="5 15">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">CYY_006761</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the pyruvate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="5 15"/>
    <dbReference type="EMBL" id="AJWJ01000326">
        <property type="protein sequence ID" value="KAF2071924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5H5"/>
    <dbReference type="OrthoDB" id="108365at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00188"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.33.10:FF:000001">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000001">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="entry name" value="Pyruvate kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018209">
        <property type="entry name" value="Pyrv_Knase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004491">
        <property type="entry name" value="PRK05826.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004978">
        <property type="entry name" value="PRK06354.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PYRUVATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="PK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00110">
        <property type="entry name" value="PYRUVATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 15" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="9 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="16">
        <location>
            <begin position="21"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate kinase C-terminal" evidence="17">
        <location>
            <begin position="383"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAF2071924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="506" mass="54767" checksum="27C410D97873E007" modified="2022-05-25" version="1">MATLSRNLRLSLDTPTQTFVRTKVVCTIGPKTMSEDALIKLIETGMNVCRLNFSHGTHDYHGQVIRNLRSAMEKTGKICAIMLDTKGPEIRTGKIEDRQGYVDLFVGQEILVDTNTSLPGTSFRISIDYKGLLDSVKIGGYILIADGVISLSITAINKDKGHVVCRVNNNSRLGENKNVHLPGAIVNLPAVSDKDIADITFGLEQKVDFIAASFIRKADDVVEIRKILGDAGKDIQIISKIENVEGVDNFNEILEVSDGIMVARGDLGVEVQMEKIFVAQKMIVSKCNAAGKPVITATQMLESMIKAPRPTRAEATDVANAVLDGTDCVMLSGETASGDYPFEAVDIMTKICREAELVESSTDYNSLFAALKLSSAKPITITETIASYSVASAIDLKADLIITLTETGLTTRLVSKYRPPIPIIAVTCHPATVKHLMATRGAIPFLVESLIGTDKLIESCLDYALKHNLCKVGSRVIIVSGIMEGVPGKTNSLRVLTVGEKEVSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V5P1</accession>
    <name>A0A8J4V5P1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003840</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000126">
        <property type="protein sequence ID" value="KAF2074852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V5P1"/>
    <dbReference type="OrthoDB" id="566238at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE RDL2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086:SF10">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE_RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="51"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2074852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="144" mass="16324" checksum="D1E87E83179442BC" modified="2022-05-25" version="1">MFHRSLGLIVKNTSSKLNRGYCSSSSFDFTGKTVINKKQLQNLIQENKDKKVKDYVLIDVREPHEVEFTSLIPSAVNIPLNNIQESLHHSLPPLNQIHEDDNIIFYCLKGGRSAAAVDIAKRMGFKNSINYPGSYIDWATEDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V7I1</accession>
    <name>A0A8J4V7I1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_001829</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000046">
        <property type="protein sequence ID" value="KAF2076862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V7I1"/>
    <dbReference type="OrthoDB" id="5337378at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001547">
        <property type="entry name" value="Probable protein kinase DDB_G0277539"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF185">
        <property type="entry name" value="WEE1-LIKE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="377"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="88"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="267"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="307"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="741"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="666"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="91"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="123"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="150"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="323"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="741"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="407"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2076862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="766" mass="86640" checksum="9F85A12DFA76E32D" modified="2022-05-25" version="1">MYPSSSSLNGNGIKSFNYRKRTPNYADFDNDDYDDDDDFNGGVISSSAFENYSFNTMMMNELKEQNNSHSNIHNNNHNHIELDSDDFRNHFSGNHTSSNTMMVPSSPHGHMRTKGRRLLFDDTPSSTAPTPTSPSSPFSSSPPTISSPPVNFNQQQTSPQIGSPMSTNIMSTNTSSKVEFKIPSPKMISSNNVNSTPYKNNIIATNININNNIFKHETMLSSSLPTSPVSWSSGSPNPYLGLSNIGGNTFPVTEVKKIRPDQRAFYESTFSSSPTPSPPPTPSLKRQLQKDYNNLIISKDQKLESNPLLLSRLGESGNRKRSTSMSSSPKPMIPSTPVNINPFTEEGKAQLNTPQANKNDNDSETIDSKFSLFKHTFQSLELIGEGSFGHVYKCRHRIDGCLYAIKKTKKPLKGQRDRQVVIREVYGLSAIKNHTNIVRYFNAWEEDHHIFIQMEYCSGGNIYSWVTDKIQQSESNLLILANQILTGISHIHSLGLVHLDIKPENIYINNSCNNSQIDLINNNNNNNNSFDININNNNNSNQLIDNNNICNFKNIVFKIGDLGLLNEATDTKIYSEGDSRYLSRELLHDDMSALKKCDIFSLGCTLYELARCKPLPKSGEEWNSIRNGFIVESNNFSKEFWDLLKLMIHPDPSFRPTAEALLSHPLIKNELNIDVLKKEMEALQAELNEKQLLYMKLLNQQQQQITQPQQQQQQQQQQQQQQQQFIQQQPQQQPAQRNIGFFTTPTNQDITHQQRQVEGGIKNMAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V8Y4</accession>
    <name>A0A8J4V8Y4_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_000447</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000008">
        <property type="protein sequence ID" value="KAF2078257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V8Y4"/>
    <dbReference type="OrthoDB" id="16606at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2078257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="278" mass="31412" checksum="4F6CFD8592A72FDC" modified="2022-05-25" version="1">MNFNQFFNRLRYTASQFARRNYSSHSISNSQIAKEHPQPKPKKSMALRVLGLVVVSKVAYDTTNTVMAYYKGNKALDTVLQETWNEAIYPVYQFAIQDKSIVAELGSPVSLGKPESQSTSPITEVSRDFQVGFYVPKPLKDVDTWYLNSIPQLSNNTATKGTIHNDKQKKEHQDKVIASMKTKLLGQQFNADLLIENAECLPFLVPYQNKTLFAFPYGETTSNLSIPIKGSKKSASIVVTLYANDDKWQIRSAAVVFENNKKVEIIQPNVQDSYLNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4V925</accession>
    <name>A0A8J4V925_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_003067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000091">
        <property type="protein sequence ID" value="KAF2075644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4V925"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="332"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="67"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2075644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="450" mass="51193" checksum="651DB534A5285FAC" modified="2022-05-25" version="1">MSRHRRNEYSDDDYEYYADYADDDFDDDDDGEGKNEIDALPSSYHNTGDQDDTELGGGASANIEQQNESSELSMNPSSSTTTESLATESTTTAASPKKTDDIITESNKNNNNHSNNNTTNSKPNGFKRDYSSRSDNSLENNVKTTIVQLPNGDTMTVPVSSSTRLYTPTHKKQSLSPSPVFHPYRGKVSSPPSQPTPIPSQIPQQQPHLYRPNALVHPNFHSPPIMVSQNNLLYSPPGISAIPHAPVSPMFHNPYSINNKNNHTHPHQPPSQPPTNPKTLYPGFINPYQVLQSQQQQYLLYLNSQHQQQQLQLQQYHQQYHQQQMQYRNLKSNNNSNIKSSDSTNTHLNKNNNNHSFNSKSIQTNHINDLKNKSLENKSKELIIQNFKDKEQIQSLLEKEMNDQNSFSIKNHSNESIIVQFKDSISSTNFKRKYHRSIQDGRQLKIDFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4VAY0</accession>
    <name>A0A8J4VAY0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Inosine triphosphate pyrophosphatase</fullName>
            <shortName evidence="13">ITPase</shortName>
            <shortName evidence="13">Inosine triphosphatase</shortName>
            <ecNumber evidence="13">3.6.1.66</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Non-canonical purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Non-standard purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Nucleoside-triphosphate diphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Nucleoside-triphosphate pyrophosphatase</fullName>
            <shortName evidence="13">NTPase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">XTP/dITP diphosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_001087</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) or xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Pyrophosphatase that hydrolyzes the non-canonical purine nucleotides inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triphosphate (dHAPTP) and xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ITP + H2O = IMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:29400"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(6)-hydroxy-dATP + H2O = N(6)-hydroxy-dAMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:83971"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:233529"/>
            <dbReference type="ChEBI" id="CHEBI:233530"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:83972"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>XTP + H2O = XMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28610"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>dITP + H2O = dIMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28342"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61194"/>
            <dbReference type="ChEBI" id="CHEBI:61382"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:28343"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="13">Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn(2+).</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the HAM1 NTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.66" evidence="13"/>
    <dbReference type="EMBL" id="AJWJ01000024">
        <property type="protein sequence ID" value="KAF2077624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4VAY0"/>
    <dbReference type="OrthoDB" id="6288734at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035870">
        <property type="term" value="F:dITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036220">
        <property type="term" value="F:ITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036222">
        <property type="term" value="F:XTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009204">
        <property type="term" value="P:deoxyribonucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00515">
        <property type="entry name" value="HAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.950.10:FF:000003">
        <property type="entry name" value="Inosine triphosphate pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03148">
        <property type="entry name" value="HAM1_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027502">
        <property type="entry name" value="ITPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002637">
        <property type="entry name" value="RdgB/HAM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067:SF9">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE/HAM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01725">
        <property type="entry name" value="Ham1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 13" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="69"/>
            <end position="70"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="145"/>
            <end position="148"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="173"/>
            <end position="174"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00093218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00093255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00093271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2077624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="197" mass="22216" checksum="3A1BD05B2C0426CD" modified="2022-05-25" version="1">MSNITKKIVFVTGNPKKLEETLAILGNTFPIESKKVDLPELQGDSPGDISREKCRIAAKEVNGPVLIEDTCLCFNAMKGLPGPYIKWFLDRLQPEGLHKMLEPWEDKSAYALCNFAYSEGPDHEPIVFEGRTEGIIVSPRGPRNFGWDPVFQPDGFEQTYAEMDKSIKHTISHRTRSLAKVKEFLRSKGYEQTPSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJM0</accession>
    <name>A0A8J4PJM0_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009846</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000828">
        <property type="protein sequence ID" value="KAF2068830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJM0"/>
    <dbReference type="OrthoDB" id="23871at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="481" mass="56623" checksum="20BB62BDDD443537" modified="2022-05-25" version="1" fragment="single">MCGYNHLGLLKEKIKRNIKLYVDCHYLYVNIANSHPDIFIQLFESYRYHALAFTKDYMKTLNNVEVIKYLVERGFGVETEIDSFERLYQIDLKALQYLLENKLCTITFRALLLKTDFRYIYSPTKARETKEKIEFLIERIKQSPITIQDSENIIESLIDHPTPLVLNSIEPFLDPGVSSILFTTSRGNRSCSYEQAVQEIRLVESERFRGEDNWILFNYPGMEEWYACVGKGEQEFINTWNRLENRIRIIDQDIEFVHSQESVSNIGERLFNIISLVTKSLRLVHFLISKGLTGFSKISALAKHNVCQSTLKHHLHDISQQDRDVIITMGRGDHLFINLVLSRFTQGYFENFNFFFKQFTQNLDIEEMHALLWSIFDTATSAKNVFLYESIEAMGNPFPIIMMNYFEFQPLLSLFGRVIDSFPEQQKNVECSTIFLRLIQADHYIGFKYVFTKCKLSPFLYIYLLEELAKSRNLVFIDYLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJX2</accession>
    <name>A0A8J4PJX2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_010190</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000965">
        <property type="protein sequence ID" value="KAF2068484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJX2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057709">
        <property type="entry name" value="DUF7949"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25820">
        <property type="entry name" value="DUF7949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7743" evidence="5">
        <location>
            <begin position="397"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="4">
        <location>
            <begin position="619"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="3">
        <location>
            <begin position="767"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7949" evidence="6">
        <location>
            <begin position="1032"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="2">
        <location>
            <begin position="1089"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="981"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="983"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2068484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1194" mass="133326" checksum="F6F1406C63B22B83" modified="2022-05-25" version="1" fragment="single">FRVLTPESLDRYASSDSNGIFKCKFSFTLAITENDQVGSIQVTAVTLTNKFSMITKKGYTYVFNFESIPFDVGLGSIDIDVNNQIGYQTTLNVPFHCNDVEFLYDTLYDSYQFNLLGPVAVFRVNNFVQEGPELTLTFTSQSLYLVKCELISFNVYKVMFGYSSGSLWGDVNNNLIVNIEHPNFATPIFNPITLKSNYISGNQITQGNLKVYPLSPTKENRYYRVYPYFEFSSTIDAPFLTVYSGVSSLISRLYPVMGNRTDGVFLNFLQVYSNTYSMVSFINNPGGVTNDQITFTSILATPSYSLTINTGSFVDGIIISNVNTNQPFREFQISPLVLSDRYISSITTPTLYPYAFSSGNAIQYQRSDEYFISPFAHGVYGFHYFNYMQEITLPSSNIDDTPPNIESVSYVTIANVIIYTVHITDDKSGFQKLIPYGNYTSLTSGTLLDGVYEFQVDLSKQVVSATKLAVCDFVLNCRDVFISSFQTSDGGNFKNDYEFKAISDINRIYWKYNNIDVSNDNVNNTLYICSSIQNLKPHLAQEKNLFNLLSSPSHSYDATLVTMGIYLDGCYEFDYVIYKNTMTGMAKYLFWMVDQTIESSYLETYFGEESKLKIKSINGDELGPLITNVQLLPGNSITIQLVDTSIEWLITIEDKFNGFKKGKITIAGDIGLVKYNFDLTTLDLISGDEKYGVYKLVVPILYKCKSQKYFIQYVELEDNMGYQTIYIQDGKYDGNLPDSMIHILHDVDTLSSIKTQCMWAQTDTEPPQLSSFTFSPNTIDTTFSGVEFDNTPRLVTFNFVVTDNEGVLNSSIPIIYLQDQSQSIIQKSSQLKSYGGNSASYQCKFNIPYGFGFPNGIKVSVFGMVDLQSNIRGYSMSQLFDAGFENTINVSPILNESISILSVTPHGRDYSQFFITGRNIRSTDLLAIQNKDYILLALNGPIINSGTFALFSYTSMPTFPFYITLRRGSNEYSNTFKVSLNESSSSSSDSSTAFSSSSDSSSMIASSDTNESSGNTSSNDQSSSGSPQECLNNCGGINNGKCKQYGCVCKSPWVGLDCSSQVIVIDPNIDPTLPQTNFTIPGTNDYFNQGANITFANQVLEMNPYSLKYSVNISQYSFSSSLNTIQLVLSASIQSSDGGVCSGIQLGDTVESESEFIKMQVDDHSLYGRFIKRGIVDGRIRQITNTPLNKEFKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PJZ2</accession>
    <name>A0A8J4PJZ2_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_010586</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001218">
        <property type="protein sequence ID" value="KAF2068088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PJZ2"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035310527" evidence="2">
        <location>
            <begin position="20"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2068088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="281" mass="29529" checksum="2174DA42F0890E7F" modified="2022-05-25" version="1">MKIVKSLLVIVLLIALVKSEEVPTAVTSIWNQDGISSADADQGVCKNVKISFTLDHPAPDVSQLSLTSIGLDSFIAIKVNGSQYDTLVTAKQGSNLNDLQVKYADKALGNIASGFTFSCTTGEATDTPNIASVVTSTTTDAPMADGFCKTILSITLDKVVDPGTLSAKSSGFPDLFVPQFVQNSTNPPSYASNITVSKGKDLSDIVFYQTTNKLSYETDAKTSTYSCSSIVAETSPTTAGVTPSPTTTGEATTGTTTGDSSVLSRNFTLTVLCFIFVSFIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKD7</accession>
    <name>A0A8J4PKD7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_010071</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000911">
        <property type="protein sequence ID" value="KAF2068603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKD7"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052050">
        <property type="entry name" value="SecEffector_AnkRepeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586:SF3">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2068603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="640" mass="74807" checksum="D9B1C47059D2FFAF" modified="2022-05-25" version="1" fragment="single">EMIFSTFKNRNTTIKSSYSYKEIISLEWIISNNKYYLLKDKIKNQCNLIVSPNVALLLFNIKDSDIFQLAFDRYRHYLDPSIPLPVIERAADKGNLYALKMLVEMGYKIKTDTESILHSAIKNNDFEMLQYLVEVLKLKSLKQQSLLHAIQSKNIEIIEYLLHNFARSIQMMDKEFILKTIAEWATLDILLLFKGFYKEITVFEYTRVIFPNSFLNISILSYIFDNNHLDTSIRGSMEWIDLIMEMIGKAIQNSNIDVIKYLVTKVDLPQSSRSTLYHLAFQKSSLEIFYYLLEKNLFTYQTFYLSDINMALVDVDSLLKYLKLGVKVGKRCLLYFVTRPYLNQEFNTVWEKRENPTTSTAHNGRVYVQGTDITLLLIQRCLENNNVFVLDFLFKNDFFPKEPEIIGYHCSFNDSSVPILQFYLSNCPHPPHIYLNFAEEIQKAIESNSIKLLDFLCQELLKTHVRIDISKLYGLACQHSCIPLLRLLKEKNISPSTYSLSIAGSPKVIEFISKNYSSFVYCISDLTLSNAISNNRLACVQYLMENHFSYFSYPSKKYMRALGESGSLDMIKYFYRYNDKFVQRPSYQLTFDIAFKCQFLDILKFLFETEKCVVNIPMAEKLSNIVKDSFSSYAIKYLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKN9</accession>
    <name>A0A8J4PKN9_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009045</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000624">
        <property type="protein sequence ID" value="KAF2069640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKN9"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="598" mass="70436" checksum="CA8B4598CDE45A05" modified="2022-05-25" version="1">MYNNRSSEFSNSDNKTILFRSILNNAYLRKHVFKYFTPPTDGYLLYYNGKFYQDTDDYNLRLLFQPNVDESFIIDKLKRFKDIVNSDNSSHDSRNRPYYQDWLDVDSRTDFKKILQEVFINSTKKPEFVALVKELFSYNGNAILGDCVPTKDKIMFDVSTVNSEQEFRMLYKANPRYFAYKQVIQNTCIHDVFEFSQRVYHVAGDKKQNVALELLASVYFLAIMDGNMEFVRFYNKLWIDGLFCRMLTSRFDLKYFITTLLMAYCYSIEVYEYLVALLLAEDDRRIVVDDRVQIYIDESTQKYYKLSLFRLAIKRHDMEKVKAIYYSWSKQEQAIHLEKNTFDHLYAALANAKTFAEKTQLMAIGEFLKSEYLATEGVYPQHSSLIYVQDKAFYTLLRSRKGKSPLAHLARFIKGALFDGNFSLLKYLVKQFTPNELTSIDIGAVIKEYTYFTHGDFLKGILYLFEVFQPCLDESILDHLMHTAMRYNNHRLINILFHRTRVRLIQPRSQHHEQLILSRMKLMCKSSPHLASCTYFEKQPNFEIDGPLLDQETASHANVSPSIRYSVFKIDVDETIKTVLEYNPSCNTFISEKIKKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKU1</accession>
    <name>A0A8J4PKU1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_010314</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01001034">
        <property type="protein sequence ID" value="KAF2068359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKU1"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050177">
        <property type="entry name" value="Lipid_A_modif_metabolic_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245">
        <property type="entry name" value="BIFUNCTIONAL POLYMYXIN RESISTANCE PROTEIN ARNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245:SF51">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE_REDUCTASE FAMILY 42E, MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="363"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="447"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="7"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2068359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="466" mass="53372" checksum="BEF0F25117935678" modified="2022-05-25" version="1">MIDTENILLTGVTGFLGNNLIIPLLERKKGQNATDAIKDASLSLKRNTLNSNSSSSSSSSKSPSEYKYNVYALVRSKSSNSKFLNDLQKKYNDRFNLIIGDFVNLDNSSNSDNSSDSQVPIIKNLSKILNDYKIDVVIHIAAMMEFFPKDNNILYKTNVIGTENLLKSCVKVNNNDDDDHDHDDDHHFSIGSSNSSNKKKNNKIKRFIYVSTTETMGGKKPVTNINRSELDDPYSHPNYYYGETKRVAEDNVRRYQSKYGLDCIILRPTGFYGKNDDFSLFEFIQAVSYGLLFFLPSFATGHVMYAHVDDIVQSVLLSITKEKLATDDTELAHTYIISPDQGLSYKETIIFLNEKLNRMKPRFQLPASIVYPVISCVGSVMYLFKKKKFLYTSETLDRMQEDRLFTNERAKRELGYRPQYTLRQGLNVTVEEYLENERIKSYPVSPLFLLTTILILVIRFVFSWFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PKY6</accession>
    <name>A0A8J4PKY6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="7">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_009655</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="7">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="7"/>
    <dbReference type="EMBL" id="AJWJ01000768">
        <property type="protein sequence ID" value="KAF2069024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PKY6"/>
    <dbReference type="OrthoDB" id="7396459at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17960">
        <property type="entry name" value="DEADc_DDX55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="45"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="293"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="544"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="620"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="620"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="638"/>
            <end position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2069024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="657" mass="75114" checksum="2648BA70A025AEAB" modified="2022-05-25" version="1">MSSTSFSQIDGGPWDSLNPTLKESTRKTIDRLGFERMSPVQAAAIPHFLTNKDVLVEACTGSGKTLAFVIPIIEMILRKETTLKKDDIISIIVSPTRELATQIHQVLLSFVNDLKSPPLSEEEQEQEILKQLDQDDQETDTKEQHQQQQSIDISTLLLIGGTEIYQDIQNYKNYGGNIIVGTPGRIDEFMSRVHQKDSLKLRDFEVLILDEADRLLDMGFHLPINSILLKLPKQRRTGLFSATQTTEVKELARTGMRNPVKIAVTVKDINTMEVQSIPSTLDNRYMFVPVEERVNQLAHFISQHIDNNKIIIYFLTCAGVDYFYKLLSSIKMFKGKPFFSLHGKAPHNQRVKVFDAFSSAKNGVLFSTDLASRGLDIPNVDWVLQCDAPQDPKAFVHRIGRTARMGREGNALVFLSPEEDTYIDFLKIKKVPLLEMEKPTTNVIDIIPEARKIASTDREIMEKGVLALVSHVRAYKEHLCPYIFIFHKLNIANLATGFALLYLPRMPELKDVVVEWSSGFTPEQIAKIPYKEKKKEKQRKEKLEKLQKQKEERKLNQKDSKKRKLEETKVAAEAKLKEKEEKEKLAIEQQKLKEQGTSLKEIEKQKDIQEILQETKRIKMAGKKSKKHIKRNQFSDLMGDDAIAEPEEEQSNSDDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL05</accession>
    <name>A0A8J4PL05_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Methionine aminopeptidase 2</fullName>
            <shortName evidence="8">MAP 2</shortName>
            <shortName evidence="8">MetAP 2</shortName>
            <ecNumber evidence="8">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_009896</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="8">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="8"/>
    <dbReference type="EMBL" id="AJWJ01000846">
        <property type="protein sequence ID" value="KAF2068780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL05"/>
    <dbReference type="OrthoDB" id="7848262at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004239">
        <property type="term" value="F:initiator methionyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01088">
        <property type="entry name" value="MetAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03175">
        <property type="entry name" value="MetAP_2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050247">
        <property type="entry name" value="Met_Aminopeptidase_Type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002468">
        <property type="entry name" value="Pept_M24A_MAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018349">
        <property type="entry name" value="Pept_M24A_MAP2_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00501">
        <property type="entry name" value="met_pdase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777:SF2">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01202">
        <property type="entry name" value="MAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 9" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="11">
        <location>
            <begin position="137"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="427"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="427"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2068780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="446" mass="48812" checksum="76656831E3AA2698" modified="2022-05-25" version="1">MTTVETKEQVPPKVVPEKEDSDEESDDEVTTAAGTDATKEGAAAGAGGEGAAKKKKKKKKKKKSAAATTTAVDANGNNVVIGLAQTTPPTIPVSKLFPNKVYPLGEVCEYKNDNSYRTTSAEKRDQERLMANVYNDVRRAAEVHRQVRKYVQGIVKPGLALTELVESLEKASRTLIEADGLKAGIGFPTGVSINHIAAHFTPNPGDKTVLKKDDVLKIDFGTHVNGYIVDCAFTVTFDEKFDNLKNAVKEATNTGIREAGIDVRLCDIGAAIQEVMESYEIELNGKTYPIKSVRNLNGHTISPYVIHGGKTVPIVKGGDATKMEEGEFYAIETFGSTGRGQVIEDLESSHFMKTPHAAQATIRLPKSKALWQHINKNYDTLCFCRRWLDDAGEKQHFMSLKNLIDVGAIDPYPPLVDIKGSYVAQYEHTLLLRPTAKEVISRGDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PL22</accession>
    <name>A0A8J4PL22_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000589">
        <property type="protein sequence ID" value="KAF2069822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PL22"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="89" mass="10115" checksum="E97CA4C1B9E708FC" modified="2022-05-25" version="1">MSSLLINNATQSPVYYYSFSPSMIHTSIPPSPPPSPISSPSMSSLNQYFIDHHHHFHQRHLNLNNNTINLNNNNNNNNNNNTNNDSKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLE5</accession>
    <name>A0A8J4PLE5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009443</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000710">
        <property type="protein sequence ID" value="KAF2069234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLE5"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="21517" checksum="AF2B5AB01A27CC98" modified="2022-05-25" version="1">MEEVVIEKSDVGIYLNKYPTELDGVVDCDEYRSIVYHLNERVKRIAVIKGISMTLAMAIGVVAGLSFKNHLYVSGLILGVTAIAFIGFCLNMTIKPQLQKFNNEFNSRGVSFRQVTYTERINDDESTSYTLLKIFYKKRQSPIGQQQQQQQSHSTFIFSEHPQGVGQPYSSPPLLPGVQTQQNEYIEIDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLS7</accession>
    <name>A0A8J4PLS7_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_009394</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribonucleoside diphosphate reductase small chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000697">
        <property type="protein sequence ID" value="KAF2069288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLS7"/>
    <dbReference type="OrthoDB" id="10248373at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01049">
        <property type="entry name" value="RNRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="entry name" value="Ribonucleotide Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033909">
        <property type="entry name" value="RNR_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030475">
        <property type="entry name" value="RNR_small_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000358">
        <property type="entry name" value="RNR_small_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409:SF18">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SUBUNIT M2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00268">
        <property type="entry name" value="Ribonuc_red_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00368">
        <property type="entry name" value="RIBORED_SMALL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2069288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="331" mass="37902" checksum="2520A7E457ACDE0A" modified="2022-05-25" version="1">MEEPILKENKDRFVLFPIKHNDIWKMYKQAEASFWTAEEIDLAPDLNDWNKKLNDNERYFISNVLAFFAASDGIVNENLATKFMSEVQIPEARCFYGFQIAIENIHSETYSLLIDTYIQDSVEKSKLFNAIETIPCVKKKADWALRWITNSKSFAERLVAFAAVEGIFFSGSFCSIFWLKKRGLMPGLTFSNELISRDEGLHCNFACLLYSKLVNKLDAKTIETIIRDAVACEKEFVCDSLPVDLIGMNSRSMSQYIEFCADRLLYTLGYKKIYNAPNPFDWMEMISLQRKTNFFEGKVAEYAKSGVAVKSTNTGSAPQSSSRTLVLDEDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLV0</accession>
    <name>A0A8J4PLV0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_009421</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000704">
        <property type="protein sequence ID" value="KAF2069260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="21427at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054484">
        <property type="entry name" value="ComC_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055462">
        <property type="entry name" value="DUF7034"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055463">
        <property type="entry name" value="DUF7035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056645">
        <property type="entry name" value="DUF7743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057709">
        <property type="entry name" value="DUF7949"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31378">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22933">
        <property type="entry name" value="ComC_SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23033">
        <property type="entry name" value="DUF7034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23034">
        <property type="entry name" value="DUF7035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24893">
        <property type="entry name" value="DUF7743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25820">
        <property type="entry name" value="DUF7949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1306"/>
            <end position="1329"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7743" evidence="5">
        <location>
            <begin position="404"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7035" evidence="4">
        <location>
            <begin position="627"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7034" evidence="3">
        <location>
            <begin position="777"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7949" evidence="6">
        <location>
            <begin position="1015"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="domain" description="ComC supersandwich" evidence="2">
        <location>
            <begin position="1052"/>
            <end position="1280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2069260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1353" mass="151000" checksum="E1558D3F667CD005" modified="2022-05-25" version="1">MALLIVDVNGFSYTDLTVSDYNDMYAQESLIGRGWECESKRDYLFNDPTGILNVDVDSPAFLYIRKMVSNTTTQALYTLVVLLKPATTTFVLNVHTNTTIVKLDVAVICELPPTPDLQFFSGDEVKFFPGTMVAQPYFLFKVKNFNKPLAPRLQVINIEGNYDAVPVGLTKNVYIVTLVVKSKDSMFGNSNFNISNGYNGYYIHQTRSFLPNYNNIILQNSIETFALNGQLFYPEVCIMNESHLDSNTPMVKFFEINNTGYLETAPLVLGNYSTGVYFRKYSVRSYGQGIFTSYTYNPNSVVNISTIGYYEDPGQKPFSLSVLSTLENQNVINVKYVTNVTLKSDLINVNGKVMVIPYPFSYSAGNSLSYEKNHAFYFEPIFYTRGSLYDGVVNPSFVLTGNLIDTEPPKIISIASLHLPNQPYVVLRMEISDYSGFNTINFGDYKDIISGTFNHGVYEFLCRQSNAMLQLYGGQVRIEDVMKNYLDFSVFNTYVNVGLEKYPFKFLNLLNIKSIKFEKNDIDTSDKSVDNHMFLYPHIVDTNVYPGFIVPSFGDILSEAVNTFTGVWNGECGCYVIPFTVPMNYPTGRFEYFMAFSGDMRNIYSVNYLVDMFGSDAALRVYSNTTDLIGPVIQDVAPQTSAVVVPDGVDTIIKWNFAIFDYFNGFKSGLITVVSSLDLVKYNFTITADDRISGTIYEGIYSINITVNGRCKSQSFYIQYAFMEDRAGKYSEYRESGPFPSSQEKQHINPFMMIGNITEVSKIDVDCQNSLLDTIPPLLGGFKFTPEFIDTANSTRQVLFEFETKDDNGILLAALPVVYLQEQRTNIVYKTATLVSYISDIAKYECLFDIPFLFGFNSSIKVSVFGIVDNQSNFNGYSMIQLQNAGFKNTINTESKLAFNLTILSTKEVYKSGGEIIIVGRNFSPLSVLIITYTNQTIRTIADPLFSSKTVVMFDMGKGEILGPNITITINDKIQYSNSFTVQVINKTIASPVFPPDSSSSDEPPVTNKPQICISDCGGLGHGVCTPAGCVCKSPWVGVSCTSQVIIIDPVINSTTPSTNITVPTKDKTTAIYYAIISVVALNELDQNGQLVQKHEFPSWIVSNNTISKYSQYTNSSYLYTSSINNNNTTTNVNVSIDYFNQDQPVNITFANQIITMNPYSLKYSVNISEYSFSSSLNTLQLVLLASIETDSNEECSSKQFGETVESNSEYIKMQVNDHSLYGRFIKRGVVDGRVKSVSNILLDNQFNSVSTQSEQQTYIGINIPFYSKSVQLDPDFSVLVDNKPASDTDDAICGKGKTKLTHAQIAGIVIGAAAFTGIVVVGVSYALYKSRQQKNFRNSLSLKMKEASSENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLV2</accession>
    <name>A0A8J4PLV2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carbohydrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">CYY_009706</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000784">
        <property type="protein sequence ID" value="KAF2068974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLV2"/>
    <dbReference type="OrthoDB" id="20980at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019028">
        <property type="entry name" value="CBM_49"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052879">
        <property type="entry name" value="Dd_Spore_Germination_Stalk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33239:SF12">
        <property type="entry name" value="CARBOHYDRATE BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33239">
        <property type="entry name" value="CELLULOSE-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09478">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01063">
        <property type="entry name" value="CBM49"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035281562" description="Carbohydrate binding domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate binding" evidence="3">
        <location>
            <begin position="26"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate binding" evidence="3">
        <location>
            <begin position="183"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAF2068974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="283" mass="31468" checksum="7F637F43FBAE81F6" modified="2022-05-25" version="1">MYKHSIFIALALIAATYSLGTPQCTVSNKCTQVHQWMNDGVMNTQFACELTNMGSKPISHVDIRLNDNQNLYNTWEIATSNNGLSWDFVKWREQKPLEVGLVHKWGYIVKSDKPLNIEVCEKGFVNPTVSPSPSNSHPTNEPTTTPDHPSTTEHNPTTQPQTTTASESVDAAQDKSKHQACEATFVQKITNYFLGKDHLRYTQVEVTVSNIGKKDLSSVIIFTDVHFNSTWGIKEVDPINAPGNYEVIKKSKSLAAGSDQIFSYVFVSDNKVAPMNLISNACQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PLY5</accession>
    <name>A0A8J4PLY5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome b-c1 complex subunit Rieske, mitochondrial</fullName>
            <ecNumber evidence="11">7.1.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_008246</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a quinol + 2 Fe(III)-[cytochrome c](out) = a quinone + 2 Fe(II)-[cytochrome c](out) + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:11484"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="7.1.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="11">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="11">The Rieske protein is a high potential 2Fe-2S protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Rieske iron-sulfur protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.8" evidence="11"/>
    <dbReference type="EMBL" id="AJWJ01000492">
        <property type="protein sequence ID" value="KAF2070435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PLY5"/>
    <dbReference type="OrthoDB" id="1637982at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008121">
        <property type="term" value="F:quinol-cytochrome-c reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03470">
        <property type="entry name" value="Rieske_cytochrome_bc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.102.10.10:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit Rieske, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.270">
        <property type="entry name" value="Ubiquinol cytochrome reductase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037008">
        <property type="entry name" value="bc1_Rieske_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014349">
        <property type="entry name" value="Rieske_Fe-S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005805">
        <property type="entry name" value="Rieske_Fe-S_prot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004192">
        <property type="entry name" value="Rieske_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006317">
        <property type="entry name" value="Ubiquinol_cyt_c_Rdtase_Fe-S-su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01416">
        <property type="entry name" value="Rieske_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10134">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT RIESKE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02921">
        <property type="entry name" value="UCR_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00162">
        <property type="entry name" value="RIESKE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81502">
        <property type="entry name" value="ISP transmembrane anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Rieske" evidence="13">
        <location>
            <begin position="123"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2070435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="218" mass="23485" checksum="6C5DD6297940F8B2" modified="2022-05-25" version="1">MLGRLTKLTNLSKNCTSIRNYATYNTVNSTTSFPTGHFEKYKADKNPADPENRGFAYLVVGSAAAGLAAMAKNTVVSALSTMSPGQDILALSSVEVDLSGIPESSAVIVKWRGKPLFIRHRNQEEIAAAQQVDLATLPDPEPDTKRAKVPEWVILVGVCTHLGCIPISGGGDYAGWYCPCHGSHYDNAGRIRKGPAPLNLVIPPYKFISEDKIIVGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM16</accession>
    <name>A0A8J4PM16_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_009142</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000644">
        <property type="protein sequence ID" value="KAF2069534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM16"/>
    <dbReference type="OrthoDB" id="24485at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2069534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="599" mass="71725" checksum="E10D09B62CA97B35" modified="2022-05-25" version="1">MRPQQSIDNQESKNFYAGDFVFWANNPNVCYEKLFKITLPTIENKSKTDSYFWYTFRNVFIRNHIWSFIHKPLVPYKRYRHIYSIKWIFKNAHSIQLLIDKVKRNEYLCDILYISNVLDQFKSINDIEFYTIFNQRYARYLCDPVMAKVKRFLDATLNNRQEQEEKEPIILFQFLSGMDKENDPSYINHMNLKQLMEEYRGTKNIHFNNFKRAFESKHLELAKFVFRNWLEDNDEGLNPRNDYGFIHMGDTEIIKYFCQVNHNNRFNLKVGLGNIYLRDDFEEIMKLLEDSGVIDKQHEEHMDGIRCKRNLEYFIKNGYAFSESLPRTVGCGDKLQFDCLPLLMNHIDFDPINLIFLFYYKSMLPRELVVYNQFFKRYTMVELKEKHPYVSERFTRYQEMYQCLYQPDYQNVNTLKDLPVKIRRIGEIGDSSAILTLIKSHFSEFFFDYSQYLDPYSPESRDPDYADDDDYVVTNHLTIELLLFALVEESNVPLLKMIFQEFPQLLDTVYKDQERVDRLVLKAFIPTSLELLDYIIDTLKLPPPSPSILISKNKKNPLILSYLLNKISPTTQSTLISNQDIIEYEKKYFCKLVSYNMNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PM36</accession>
    <name>A0A8J4PM36_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_009138</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000643">
        <property type="protein sequence ID" value="KAF2069547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PM36"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000003">
        <property type="entry name" value="Calmodulin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13405">
        <property type="entry name" value="EF-hand_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="8"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="79"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2069547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="147" mass="16462" checksum="85071E05149D2769" modified="2022-05-25" version="1">MSTKLNEEAQAEFKEAFTLYDSNKDGRAEPAELGNILRWLGQNPSSSEVAEIIRDYGSNGAITIDGLYNYLGRKVVDDFDEREIIEAFQVFDKDGKGMVGASELRHILTNLGERLPEDQVEEMLRQAVGAGDGVISYEPFVRNMLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMJ6</accession>
    <name>A0A8J4PMJ6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinase</fullName>
            <ecNumber evidence="4">2.7.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_008731</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the inositol phosphokinase (IPK) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.-.-" evidence="4"/>
    <dbReference type="EMBL" id="AJWJ01000567">
        <property type="protein sequence ID" value="KAF2069954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMJ6"/>
    <dbReference type="OrthoDB" id="21029at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000828">
        <property type="term" value="F:inositol hexakisphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.160">
        <property type="entry name" value="Inositol polyphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005522">
        <property type="entry name" value="IPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038286">
        <property type="entry name" value="IPK_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400:SF21">
        <property type="entry name" value="KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03770">
        <property type="entry name" value="IPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="7"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="112"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="375"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="491"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="14"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="112"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="382"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="415"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="495"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="537"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2069954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="549" mass="60952" checksum="EBB8DA911FC4998E" modified="2022-05-25" version="1">MHSFYLVDSLSPLNSSSSSSSSNSNSNSSTSNNNNSNSKNPDDRTIINQFNNQVGGHSAILSIQSKICKPLIHREHQFYEFTSLKNNIHSFLISPFIANYFGKIEILKQSNNNNNNNNSQNVNNQNNNQYISGSVNVKNNNNQNNQQKNSKHQVGTKQQYLLLEDLTTNYSKPCIVDIKVGTRSRGTICSSTTSTSLGIRVCGMKVFNPQLGNHIIYDRYYGRSLTPSTFEDSLYNFFIGNDHDNLGLVQLYQKLYLLDNILEKLNHLYVVLSNNNDPFPFKIYSSSLLIIYEGDVDINNSLDDSSNNNMITNDNKNNSNPMYIQSLNPLSSSSSSFASSSSFTGSSLSSSSSPSNSPCTVYNSVFKSNVSRNSVSSSASCEDTDSIESDEEMDDSAVSSDLDSDSESDEKSFDTNNHFINHLSKNSSNKNTTLLKQIQHDVKMIDFAHAIPKHEQDAIEDDGYTFGISNLIMILLNIKTKLQTSSQKKLSLPFSSNNHNINNNNNNNNNSINSSANGSNNNNNNNNNNNNNNNNNTFIPNSLIPTLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMP3</accession>
    <name>A0A8J4PMP3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">UbiA prenyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">CYY_008640</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000552">
        <property type="protein sequence ID" value="KAF2070044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMP3"/>
    <dbReference type="OrthoDB" id="203513at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042371">
        <property type="term" value="P:vitamin K biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13962">
        <property type="entry name" value="PT_UbiA_UBIAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026046">
        <property type="entry name" value="UBIAD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929">
        <property type="entry name" value="1,4-DIHYDROXY-2-NAPHTHOATE OCTAPRENYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929:SF7">
        <property type="entry name" value="UBIA PRENYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005355">
        <property type="entry name" value="UBIAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAF2070044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="34714" checksum="73F7CD5687666D86" modified="2022-05-25" version="1">MFGHLLRFIKNYFIAIRLWTVVFIFSLSFLSLCIFYKDDTKIDAYIFVVVFFIQNLLVFLTNLVNTYYDFINQVDKAETSADRTLFDYQITLRDVQTYIYFTLSLIVLGMALLYPTMDPFTFYTKLLPLVLFSIIISYSYTAPPLVFKYKAMGEVSIILGISCLMPLNFLAQNHYISWKSIHFSMIYSLGVELILHANNHRDLEKDKQTGIRTLAILMGPTLSYIFYFSIYAIMFFYCYCLSILYDNVYLNIPLISIPDIFIIYVQTKNKNFHRFDQKSSKMLLEIVLLLSIGVLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PMR7</accession>
    <name>A0A8J4PMR7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Letm1 RBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_008598</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000545">
        <property type="protein sequence ID" value="KAF2070089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PMR7"/>
    <dbReference type="OrthoDB" id="275278at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033122">
        <property type="entry name" value="LETM1-like_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044202">
        <property type="entry name" value="LETM1/MDM38-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009">
        <property type="entry name" value="LEUCINE ZIPPER-EF-HAND CONTAINING TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009:SF1">
        <property type="entry name" value="MITOCHONDRIAL PROTON_CALCIUM EXCHANGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07766">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51758">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="220"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Letm1 RBD" evidence="10">
        <location>
            <begin position="264"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="494"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="494"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="507"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="554"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2070089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="579" mass="65039" checksum="CB9612AFA1D1C902" modified="2022-05-25" version="1">MNSMIVHSQTANVAAQVGKLLVQNGLPAMMTSTLTSRKYTHLSSSYNNTNRKLNLVSQQQQQQPKQSKSFINLYNNNNSNIINEINSKHFLNNSSYNRLFSTSTTPPPTDKQDTTATTTTANTTTTTTAAKETAVDKTATIKRKYSEEEDVIVSIEPKPEKGDKKDLMAKIKGVANHYWYGTKLLGKNIGIGIHLIKRIIKGHTLTRRERRLLSQTSADAMRIIPFIIIVLVPFLEFALPIILKFFPNLLPSTYTHENEKLEGKTLRSKGNMSVQAQLKDLLHELSVDSKKNTNSTEFYDFMTKVKMGETVTAEQVLKYSKFFKDDIIMEKITRPQLLSMHRYLAGGSPLTQWYSNEYLKDQIYKKLEKIKQDDILIKKEGLNSLTLEELVDAAIVRGFKVEGYNRKFIEGQLDQWLDLSLNKSLPPSILILSRAFILSPNLSAPEALEDTLEHIPQQILKEVAKELPADLSTEAGVEMAKEKLDELTKEQAEIKVEESQEEKEAAEAAAAAPEKVESTTATTTIPETAQPEREDLKKKSHSVSDDDDIEIHGNGNNNNTNNSATTTTTTTESKNDKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PN84</accession>
    <name>A0A8J4PN84_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008331</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000504">
        <property type="protein sequence ID" value="KAF2070344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PN84"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="185" mass="21611" checksum="39D6AB9D25EB950D" modified="2022-05-25" version="1">MISQSNVNINFLNHEEDEDDDDNYSGDDVHHDASINDRKNDDNKCSIKSLSCGQQQQQQQQQLQQKQRRHKEQKISDFKILTKKTVQHINVGFIEIGDCFEKINQCASLRSITITIILLSYHKLEVLNKLKRIDKINVNFFLELNLPDDEPEILPEIPAITFKFKTLLFETEHYSGDNNKSFNFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PNW6</accession>
    <name>A0A8J4PNW6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fungal lipase-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_007961</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000454">
        <property type="protein sequence ID" value="KAF2070719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PNW6"/>
    <dbReference type="OrthoDB" id="345705at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051218">
        <property type="entry name" value="Sec_MonoDiacylglyc_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45856">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45856:SF11">
        <property type="entry name" value="FUNGAL LIPASE-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fungal lipase-type" evidence="1">
        <location>
            <begin position="111"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="307" mass="34195" checksum="4CD5F26411DDAAA6" modified="2022-05-25" version="1">MLIKKNNSFSSSLSVLFIVFLFVTLTISINVPIQPELNVPVPKPFSLDTAFSHLLYSYATYCSPLKVVNWTCEYCSFKEINPQLKVTAILENVSTNTFGFIGYSQLNQTIVMAFRGTQMASLQNWITNLNFPKTEPYPKFPSALVHSGFLSAYQGVKSQVESGLNYTQELCPSCDRLIITGHSLGGALAILAATDVYESGLTTLPLEMYTFGSPRLGNVAFAEYFETTISKNLYRLVNDHDLVPHLPPMDLNFHHLPIEVWFNTTSVPLDYYVCNSSGEDPNCSDSLTVALNIPQHLDYLGIEKALC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP14</accession>
    <name>A0A8J4PP14_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_007948</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000452">
        <property type="protein sequence ID" value="KAF2070733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP14"/>
    <dbReference type="OrthoDB" id="19753at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507:SF1">
        <property type="entry name" value="FI18259P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507">
        <property type="entry name" value="ZGC:174356"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="62"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="319"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="348"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="372"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="406"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="439"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="72"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2070733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="484" mass="53878" checksum="9F8A3D81294E2F55" modified="2022-05-25" version="1">MENSQGDAVLVSDVKYEEEIFIPQQKNNLKNSKGGYFMIDNEQGPNSNKSISSRPKYLKDILLIYFILTFFLMYNILFAIFIPGMFTNYVNFKYPNLSEKEIQSKSSHLKSIADAAPLCMVFFFGPLLGGLSDKYGRKKILLFQIILIAIDVSSAFISFKTNSIIFFYIGHTICGLTGCVGSTILSSISDLTTIEERPKVFSWGGVAMGVGFIGGPLLSALAMKLNVLAPIYFIYFTIAVMLVLLYLLKESIYLGEKGKAIVKTTFNPFKAIARLFKSSLFVALVAGLYVIFAFASDDLLSTMYYYTEVRYNWSPEKNAYYIAYLGVVIVVYSSLIIPFALKYLSERAVISTALFMNGIAHIIFAFASNQYIWIVSGLFGGFPTVILMIIVSIISKSTPPEIQGTILNGVASVGSLTALAGTLFSQNIFAYFISSNAPIYFPGAHFLIDSIPIFLTFLGSILIWKFYPAKQMDKGFQKVQIQEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PP86</accession>
    <name>A0A8J4PP86_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_008455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000523">
        <property type="protein sequence ID" value="KAF2070221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PP86"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035193124" evidence="1">
        <location>
            <begin position="20"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2070221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="306" mass="33918" checksum="69663A60E605DC3D" modified="2022-05-25" version="1">MKLLNIICALFICISVAKATYPQNFYYYNSSGIYITPSENSDNPTLVPVSSSYRQLGAITKVDSQNLYAIVISDWDIVDYISIDLSSGETNTIQQIVYSNNILTGPLWGYSLDLKKNVLFSLSPTSAFHIELLINNLDTQNQTALSFGPNGVSGEVVSSYDQSTGMYYALGPFLQQFRIYVCDTNALSCKNFIVNGLAYNNQKYDIAAFNGQLFVFITANSNILIYTLNVHTNTLSLRDTIALDNGNIVFVQSKYNFDENILMVLGTTNGPNALTFVNIINTNPVEVNGGYSIDQLIPTYSLIYVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PPQ9</accession>
    <name>A0A8J4PPQ9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Pectin lyase-like family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">CYY_007981</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell outer membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000457">
        <property type="protein sequence ID" value="KAF2070700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PPQ9"/>
    <dbReference type="OrthoDB" id="24297at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003368">
        <property type="entry name" value="POMP_repeat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01376">
        <property type="entry name" value="POMP_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31318">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035282988" description="Pectin lyase-like family protein" evidence="10">
        <location>
            <begin position="26"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="498"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="459"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="557"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="561"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="602"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAF2070700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="613" mass="65440" checksum="CC204B80F6485B0F" modified="2022-05-25" version="1">MNNLSFYLFAILSICCCCFISASKADLVLYVDLNSAFKDQQCGNSVQNACVSLGAAYQSYALQTGGSNKTTLTLELLDGVYDVAANNAMPNTNNIYINILNINSYSNNSKNVVLNGYQPGYTLFSYLQQSNQSIMLTISNITFANSYAIVNSTSTLSLTFVGCVFRDINQSAALSLVSLTNFNNTWKPTVAFIDTTFSNISIGDTTMASAIKYTFLLSNVNVNNCSIRSAFFFSGGDGTLDGVTISNTAATLAPISLSMSGDISVMDSLFNNNTGLAGAIYFFDQVTYNALIENSNFTNCKSSSNGGALVLGANSATFKFNNCNFIANSAKYYGGAIYATNNFVNVYHSTISANRAYNGGAVFADTGSNIMLSETTVNSNYATQGGAFFSYKASVTLASDSLSGNSAQTGNDMLCVSSTIGVSPPNAIVDKNYYCLAPCTFTSPENYQCIGSTTGSTVTTTSSSSGGSSTISTSSSTSTSTGYIPYINPTNNDDREKLAVGIGVGVGGFILIAIILLILIRCRSRHHHHVATGYTSVGVVGTSGSVGFGAHTTLNPTPVFTHHHHSGHHHHHGHHGHHDHHHHGHHDHHHHHHTESSSLIDHHHHHHHHGNHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQE1</accession>
    <name>A0A8J4PQE1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phospholipase B-like</fullName>
            <ecNumber evidence="9">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_008465</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Putative phospholipase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the phospholipase B-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="9"/>
    <dbReference type="EMBL" id="AJWJ01000524">
        <property type="protein sequence ID" value="KAF2070219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQE1"/>
    <dbReference type="OrthoDB" id="443524at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007000">
        <property type="entry name" value="PLipase_B-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12370:SF26">
        <property type="entry name" value="PHOSPHOLIPASE B-LIKE PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12370">
        <property type="entry name" value="PHOSPHOLIPASE B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04916">
        <property type="entry name" value="Phospholip_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7 9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035341454" description="Phospholipase B-like" evidence="9">
        <location>
            <begin position="20"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2070219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="560" mass="63380" checksum="1EEC39F9A615D862" modified="2022-05-25" version="1">MKSQITVFLLSLLIGQLLAIQINSDQLPFGKADKTAFPTFTVYQNSDNGYIVKPVKDTTGIAYAVFSNEMMTTGWGYLSITTNSSYSDLTQAAGAGYLEGFITQEMIWQNWVNMYVNEYHKTIGQDVQNWITSNIQYMDAQINANPTDPYWTHISLIWTQLTSMVSGYNDANTDSSTSLQLLDFLLMNMDGDMIDLGPALNLSSSTQTKEFNPKVRFTEFMRKTGHCSALIKLADDLSDLYSAHVTWSSFYEMSRIFKVYNFGYKNPAASKITMFSSYPATLSSIDDFYLLDTKITVIETTNGLMNNNLYPLITPNSVLSWIRVIVANRMATDGNSWCQVFEKYNSGTYNNQWIIVDYNKFTPGVSVRDGTLFILEQIPGYCEYADNTDILLTGYFGSYNIPRYEYIFNISGFNDTSAYGNWFSYTASPRAEIFRRDANNVHDLAQMQAMIRYNNWAKDPLSLGNAGNQISSRFDLVTHDDPSNQYLNPDAFGGIDGKVVNYQYVQNLLVAAQSGPTHDQVAPFDWTQNGGVWSSKYTHVGMPDRWDFGWATMSVETMDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQQ8</accession>
    <name>A0A8J4PQQ8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">O-acyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY_006333</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Endoplasmic reticulum membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000287">
        <property type="protein sequence ID" value="KAF2072363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PQQ8"/>
    <dbReference type="OrthoDB" id="10039049at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014371">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408:SF8">
        <property type="entry name" value="O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000439">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="276"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="381"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="407"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="451"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000439-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAF2072363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="473" mass="56242" checksum="ADB58BAF4A1B6FAD" modified="2022-05-25" version="1">MSTSLTSTLNVKNRNDKHFPHSRSGGGSHLKETKLKDEQEYHEDVIYIEPKTYNRKFTISKAYLDLKDAPGQRPSLHKYIHQNRILIFSLLLLYIALDEKNHGLFKLWSHLTIFSKIFVNPFDLLYDLIFIHTLSFFIPVVNFLHRYKKKAFNLKNLLLSYLLIQMLIIFYSYNFIIKQSILPVGSLLYMGMEIIVVIYKGHSYFIVSNYDELHKRPKKNDDFEKYNKTLFGYVKDYLHFLFSPTLIYDQYFEVKHFDYKGQESLTERVISFFKELYSSITIMIFIHYLHFEYLFPILFNESPFLAYFKILIPAELTFQLQYYLLYHCVLSLLADLTGFRDRLSFYDNYWAAETTKEILQRWSKPVHSWLYRHCLCDMRSLLKLNGSFCLFFTMLFSGMAHEFIVTSTSGGVCVPWSTLNLVGCGILISFENKFIRSQNTISRVYKTIMKIFLFMGHGLFYLAYYNFCYSHLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PQY8</accession>
    <name>A0A8J4PQY8_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sister chromatid cohesion protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">CYY_007442</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SCC2/Nipped-B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000397">
        <property type="protein sequence ID" value="KAF2071240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="418242at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090694">
        <property type="term" value="C:Scc2-Scc4 cohesin loading complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061775">
        <property type="term" value="F:cohesin loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140588">
        <property type="term" value="P:chromatin looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034087">
        <property type="term" value="P:establishment of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071169">
        <property type="term" value="P:establishment of protein localization to chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23958">
        <property type="entry name" value="SCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026003">
        <property type="entry name" value="Cohesin_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024986">
        <property type="entry name" value="Nipped-B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033031">
        <property type="entry name" value="Scc2/Nipped-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704:SF18">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN DELANGIN SCC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12765">
        <property type="entry name" value="Cohesin_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12830">
        <property type="entry name" value="Nipped-B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Sister chromatid cohesion C-terminal" evidence="11">
        <location>
            <begin position="1630"/>
            <end position="1803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="10"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="265"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="474"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="916"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1568"/>
            <end position="1619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1852"/>
            <end position="1873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1984"/>
            <end position="2025"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="10"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="278"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="495"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="514"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1575"/>
            <end position="1585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1589"/>
            <end position="1609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAF2071240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="2025" mass="229619" checksum="7F6C7330427CC3DE" modified="2022-05-25" version="1">MDNQYLYSYNNNNNNNNSNNNNLNFNDENINTINNNNYNSNHSNHMANDRYKNMPPPPPPQHTMINSHYYQYNNHSNSSNGSNVNIPFGWISNMTQLSDPINEVPLPIIRQDSSLYLIDSSNLTTTVNLLNSVNNNNNTMESSRDGFDANSSKTLLDSINQMIDNTNVSNIRIRADLPKNQAEQSPLLRNIYQHVPAIIDKDYQTILPQQLLDTMTNIINNQSTSATTTNANDSQFISDSNIFEVMEERENNFIIPYKSHLDIFPQQQQHHQEDHQQPQDIKSATASTKSTTTTIKPPREKKERKRKKKQTDEEEQQAGESNETPIQKIFNEILELFEKIKLDLRKNIYNTGLIENLGKELLSCSKLGLLKDIPIEKYAHIIQFLIKFSREGLNTSLSYNSKDKNFALYYQRVEFSLECSLLAFTILTLPEIGNEIVQLLEDQLGEIFTSIKYNVFENIIGVLDPSFKTFKATEEKKQSTATSTTTKKKRKSIDTELDDSEDEVVDEEKQKDGQEDDDDESDMDNQQDDDDDDDDDDDGGVKKRSASKKKSIIKKKSLFSSKKEENKYTNLCNKVSEIFERISCVFESGKVSLQESFIEMVLDGSLPSLFVDGLTFVQLSCIKMVRGAFSMYPKFRQTIVDEILANLSKPILGANNKKIPKHYKVTKDRSIQILSALLIQMVQSAATLPHLDSVAATSNSTDTQDSTSNSTNKFSLSECQKYATCIILFFIEKCSEKTEEGEWKTLLENFIQDLLTVLNLPEWPAASLILRLFGATAMHIVSQQKVENNFRMISIDLLGNILTKLKQEIANARLEDSLLLLNSLDTPIAATTSSTSLTQPTKVKCVCKKYCADSFTVKCDHCDELFHDLCAPSYTADHQGGAGASNTMHQWTCITCRVKTQIGDYSNSAKVTYISNDTGPSVPTLPKHLSEKKKRRKSEEGNGGSTSPASTPLVVHEKDVYQQIILNYLSTKCSTEVWVNSSKQFLIGSWNDSNVQQQQQQQQQHEIVRRHLISQWNNNQVNSKHFICTDDEAIKVFRKLAVRGSVFLIINNLLHHLLSILFDQSIKARAKAMKAFSTIVEADPSVLADEQVHRAIRNRFIDESISVREHTVELIGKYVLIKPELTWRYIDLICDRISDKGISVRKRVVKTLHDICLAQPNHPKIPEICKVLVTRITDDDAIRDLALKTFQDLWFSDTKLESAAGGGDQHPTDGAESKQELTKVRQIIEVSKYLDNDAWIVDLINKMINKDTAIGVAKKDSKKKLEKEQNEINSMCSKICSTLIEMIVSMEEKRVPKNNAQYIGIFKTLYIFCKVKPSFLVPFARFIHPYLKPANLDQPIPADEVTIYVIAAHILEKVVPLIENNDKAFISTVESDLLTLINRQGSPALIHVCIKTLCTLIQECSLNYGLVETLLAQCIVVVQSPQGKTKGDIVRCLFSIGLLIRYFNFEQRGFTTTTNTVIQPGQVVNHITPLIIRIFNYIKDPDIISKSLESVGNIIISSPVILNKDYIKEIISKAFNIGSSTSNTSSTSSTSNMVIQNQESLIRETIIKVFYDLLEKEFKSYQKDKPAVKKNEKKPKKPKKRKESDDDDDDDDDDDNDNSEDEDQDTEKKPIVSMQVDQNSESLVTAVQKYFPSMIKFLLDTNEMVRSGTISTIELIIKQGILNPLDSVPFIIALLTDPNPKISDKAYSTLLILNQKHTSSLFKRITECVQLTFRFHKSLDTKPPHHSVTRGISKFYSLFKSSKSNRTTFLNTLLSNFDTIKDYKYKELSYFKFIFEILACIPYANMDEIIVVINVIDKIVSLNANFIQSILQTYLIKATNASNNSSSTAPPTATIIDVVSETNIETETVKPVTRRGRKPAVSKTTTTTTTTEEQPIDLEEVYKNFIMIIVLILLLQLKQFLLKTYSINKEKMKSYLDSETKDSDKLTITYQDSINLTENTYPFLSLPLDQDLRSNLLVQKNLFSQLKTLLKEDDMDEFSIKTKKDTESKKRKPRAKPAAKPSAKSAPRRKKSKVSSSEESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PRL2</accession>
    <name>A0A8J4PRL2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase M24 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005636</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000230">
        <property type="protein sequence ID" value="KAF2073053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PRL2"/>
    <dbReference type="OrthoDB" id="5876363at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01089">
        <property type="entry name" value="PA2G4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000029">
        <property type="entry name" value="Proliferation-associated 2G4, a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004545">
        <property type="entry name" value="PA2G4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047113">
        <property type="entry name" value="PA2G4/ARX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00495">
        <property type="entry name" value="crvDNA_42K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10804:SF11">
        <property type="entry name" value="PROLIFERATION-ASSOCIATED PROTEIN 2G4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10804">
        <property type="entry name" value="PROTEASE FAMILY M24 METHIONYL AMINOPEPTIDASE, AMINOPEPTIDASE P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="2">
        <location>
            <begin position="36"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="393" mass="43106" checksum="B6E0D103F42C70D1" modified="2022-05-25" version="1">MSHKHHDHIDCSSEDEHFDDEDVVEDLSEPAVVSTYQAASVITNKVLKHVISLCTEGKNILDICTEGDKMIEDESAKTFNKRKGIEKGLAFPTCISVNNCVGHFSPLKGNTRTLAKGDVVKIDLGCQIDGFIAVGAHTIIIGSSAQEPMTGKTADAICAAHYAMEAALRLIRPGNKNTDVTDAITKITNAYSVNPVQGILSHELKRFTIDGEKVIFAKVEPGQKAQTHEFQENEVYCIDIVVSTGEGKAKEGNDTCTIYKRNVDQQYLLKLKSSREFISEVNKRYPALPFPLRNFDEKKARMGLSECVNHGLLTTFPVLYDRPGTTVVQFKTTVLVLPSGNQKIVGSDLALPFVRSEFSIQDPAVQEILSRSLKASKKKTTAASTKPTAMDCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSF8</accession>
    <name>A0A8J4PSF8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_006100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000267">
        <property type="protein sequence ID" value="KAF2072577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSF8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2072577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="160" mass="18045" checksum="BA9A83A07EA778C7" modified="2022-05-25" version="1">MAQKLYISKDSIISHLEEYSTIEFNSEKETDAGTLLKLNINNVETSITLNPNGTILVQSKNQNQSKRIQDALNQLNILERDNNSSIGILRNKPSSNDLGSSHDVKKYKVLVIPKNSLDTIQPRLKELKVEIEYIHLNGDEKEIIDKIKEHGSGTTLVTYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PSI5</accession>
    <name>A0A8J4PSI5_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">O-acyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">CYY_006268</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">QSvi11</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000282">
        <property type="protein sequence ID" value="KAF2072411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PSI5"/>
    <dbReference type="OrthoDB" id="10039049at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004144">
        <property type="term" value="F:diacylglycerol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014371">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408:SF7">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000439">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="410"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="482"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="507"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="535"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="121"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="14"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="29"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="76"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000439-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAF2072411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="576" mass="66854" checksum="A0402DAD7176099B" modified="2022-05-25" version="1">MDVPIFESSPQRHQQQNNNTTMKENNNIKSKERETERVRNRFISHEFHKLDRTQSRINPPKVSDTDSESESEYFLSPSQSNGNNNKSSSTAYDKRSLNGKPGGMTISQRNVSNNTKAISLDEKQQQSVAPAIQSQQHHHHHQTKQSNEKPNLTLNGLFTHRPSLLSSEASGSSYRGFLNLLVLLLITASFRLVILNHLTYGVRIDLNLLKISEYHRWPGVMIGLGINIFIISAYLIEFAASKRIFSDSFCYVLRVLNCAASVIVPSASILAFSPNPASGIIVMIMVCTFSMKIISYHYENSKQRKLNPDNTKFIYNGDTSIYPNNLSLRSTYWFMLVPTLVYQLSYPRSNRIRKGYLARRIFEALFLSSLIFWMVEQYMVPLVQNSVLPMEQYDVARILERIMKLSLPNLYVWLLGFYVFFHLYLNIVAEITRFADREFYRDWWNSTGLDYFWRTWNMPVHHWMVVLIYTPMRRRGFSKNMGYFMCFFVSAIFHELVISIPFHSFKLWGFFGIMSQMVLIALTKGSLNGKNIGNVIFWVTIVLGQPLIVLLYYRNFIQEHPSLYPTTTMMPNSDNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT15</accession>
    <name>A0A8J4PT15_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CHE group protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_005366</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000212">
        <property type="protein sequence ID" value="KAF2073313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT15"/>
    <dbReference type="OrthoDB" id="515064at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006939">
        <property type="entry name" value="SNF5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10019">
        <property type="entry name" value="SNF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04855">
        <property type="entry name" value="SNF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="84"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2073313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="412" mass="48432" checksum="5AE535A85FF90F29" modified="2022-05-25" version="1">MNDDELQQQEEQQDENVDIEEEEDEEEDINTGFSTIDTSAINTPGTTTTTTTSVNGEVDEGDDEGDDYYEEEDEGQGDNGGQGEEGEDEEMDEDDVDDEEMDDDEDEEEEEEEEEENEVEVDDDVDDDDDDADDDDQTSKQSKHPKELTTEKESSNEIFKRNDPSDDYYIPIKLDIQSGVYKLGDYLLWNLNEQEITPEHYSKRLCIELDYPEWFEGLITNYINLAINHHRNIILEFRKNLPLLKQEIKECIVTIQIDLNVNQLYLKDQFEWDILGPNSPEIFARSLALDFGLPREFEGLIAYSIREQIQLHYYYLNDHFKNNGRFLGYANMRSQTLSHDQLVRNDYQTQLFTPSVSYRQPSKNQQSNHPLQAYLMQQTQLKNQQHQIQLQQIQQHQQQHLQQQQQQLHYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT38</accession>
    <name>A0A8J4PT38_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005690</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000233">
        <property type="protein sequence ID" value="KAF2073012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT38"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035209010" evidence="2">
        <location>
            <begin position="20"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="75" mass="7830" checksum="719B3C7177A8A066" modified="2022-05-25" version="1">MKFLLLVTILFALLAVTMAINKKAIPFTNAPIFTGGSGGEDHCSDVNDFCGMGQGSNANPHQSTTGGQSGEKGQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PT97</accession>
    <name>A0A8J4PT97_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pesticidal crystal protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">CYY_005233</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000203">
        <property type="protein sequence ID" value="KAF2073448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PT97"/>
    <dbReference type="OrthoDB" id="10538590at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.10">
        <property type="entry name" value="Pesticidal crystal protein, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036716">
        <property type="entry name" value="Pest_crys_N_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56849">
        <property type="entry name" value="delta-Endotoxin (insectocide), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAF2073448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="657" mass="73804" checksum="339DE9A91127BD97" modified="2022-05-25" version="1">MSASSLYPLDVNGEDYKFIETDFGIKKGDKKNKPKSLKRGLDMVDKIVNYDKEELIQLIKGEANWKDYANLGTSLFSAAACFIPIAGPAVSAVIDVFFGLLFEFVASTEPKKPTPEEIMNDIAKQKVIDFFKGLTTGKLDDVRTALGELQSKTLSLAKNNAKVINISNFTSQYNILFTEISSLFNQTINQPMEFRLTTYNLFTFAVQIYMIRVISLTGMRKTLALTPEFLKNTLYRHIDIAQFLYLRNTYVEAYAKLKELENGQDLFAYLDTRNTILQNCPSIYNLMFTDPQLFPDGVFVENPIVFFYIYKHSNYVNYSSQIVRIVLADMKLRSNYFLYTGSLTEINCRFNASGQFREFNCVGECNRYSGFYPVYNQIKDSQSTIQKKYEIDTSLLKSFSIGLGNTSGNLIVETSAGTVTLAPGVKDAIYPTTQMNFNDVYLPNTKVVHIGTFKTSNFIDSRGLVPFVGVVDKTAEFRNFLYSSCATVINPGKMISNMKCFVNFDHLYIGYHTITVLGPDGYLEYELTPKNPAVITFKVYVQITCGYFYTGAQNDSNFTNAINKAKEKALDNTVPLEPLVKVSEGTNVDCKMVTTKKTKVQQEWAIGKGFKSKLVEVGNINLDTPGSNKLKIACTVANSGFIVEAIIFKPVIKVESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTF6</accession>
    <name>A0A8J4PTF6_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">WD repeat protein mio zinc-ribbon like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_006452</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat mio family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000298">
        <property type="protein sequence ID" value="KAF2072229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTF6"/>
    <dbReference type="OrthoDB" id="341486at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16691">
        <property type="entry name" value="mRING-H2-C3H3C2_Mio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037593">
        <property type="entry name" value="MIOS/Sea4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049092">
        <property type="entry name" value="MIOS_a-sol"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031488">
        <property type="entry name" value="Zn_ribbon_mio"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16453:SF9">
        <property type="entry name" value="GATOR COMPLEX PROTEIN MIOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16453">
        <property type="entry name" value="WD40 DOMAIN-CONTAINING PROTEIN MIO FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21719">
        <property type="entry name" value="MIOS_a-sol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21720">
        <property type="entry name" value="MIOS_WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17034">
        <property type="entry name" value="zinc_ribbon_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="358"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="MIOS-like alpha-solenoid" evidence="7">
        <location>
            <begin position="560"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="domain" description="GATOR2 complex protein MIO zinc-ribbon like" evidence="6">
        <location>
            <begin position="911"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="97"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="443"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="106"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="454"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF21719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2072229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1029" mass="116107" checksum="1B25ADBC5A9BD1E3" modified="2022-05-25" version="1">MSTVQNSTSRKRIIQWSPHDTNSFIVGSNDIRLYNIKYTDSFDNGIYHHSHHHHSSTNRQQYINEDSAVALDQYSNSVSSPSSPYLINNKLSHHNSTGSIANVGKNFNNNNNSNNRFSLPQQNHHNHQHGNSNSHSHHNSSNKDQSNNTGAFSYDIEYPQDGWEPKDKKSISLISVNSEVQLMKCMSWSPDQSDPNLVACGLTNGRAILTSFSSVNRILKEFAPRHIRACNTLAWNPIFKHQIAVGLDKVRGDMSTLVWDINYINSFKQQQQQHHQQSIKGGLVDAPHTISQISDNSFSTITNDYTETIYQPLSEFTLSEATLSLAWLPNSPHCLMVGTGSKWLKIYDIRDTNTSQALTTHQKSVSGLCFDPFDSNRFATMGEDSLVRIWDLRKFDESILTINTNCKSLQQIEWCPTRSGVLATVGKDKNTIKLWDIKSPIDLSKSPKPDRKQSISNYDPNTITKPTKTHHSTEIIASFSWHPTNECRMLTVSYSGVIEVVSMNENIPISWSPHGNLSFSHGNHLLEGPSKGTTIEPDLNIYKNLKNEDGRYDRDISLKMKERAKFGYSANVEENVNLGTKINDEGINFLWNWISKTPGQIRTHKKKMLINEETQKDKNDFVGIHHILFDSDNYFLNPSVENVCGFLHYKSHNRFLCSSICGWGVSPSLPLELIISKLEKQYLYEKAVAMAVFHLDIKKAMVVLNNVSNLSKSALSPSLSKDREFNLKVLSIALAGFGDSGNTNPIWRETCKSTAKSFSDPYLKTCLEFLSTSDSKEIYNVIEDSSINLADKIALACRYLDHQDLSTFIDKNTLAVTEMGNLQGVLLTGLTEKGIDLLQNYIDRTADIQTACLAISVVVPKIFRDKRVSKWHSIYSDLLDHWSLWHERATLDIQRAANKAEPTQPQIFAKCGYCQNSFSFEAVTAASMVGGRSQARPNFKSKVPCCPSCKQSLPRCCLCLLPLNCMVPAVDYKKPTDRESWKAGAEPFEDWFTWCQTCRHGGHAQHILDWFQEHSVCPVTDCTCRCSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PTL8</accession>
    <name>A0A8J4PTL8_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005856</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000244">
        <property type="protein sequence ID" value="KAF2072845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PTL8"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="498"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="571"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="371"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="572"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="630" mass="72501" checksum="F2482FE5A34A4A5F" modified="2022-05-25" version="1">MKKPTRAEKDILNLLPQALEGGRTRRTITPIHDVSEIAIPMKSNATTKKAAAAASKKKTKDDNSDDSDDDDQQLSTKSKTKISKTKKTTKTKKPTTVNKKSTIAKNTKKQTPKSTTSQKRKKDQESESDDEMDIDKDENSKEEEEEEESDVESEELSFMSDSDSHIDSDSDIDYKSKKKQQSSTRGKKKATTKPSPPKKKAKKSTKVAAKKQTKKWYENDSDDDDNSDGSDNDKNNSQPNWGFNTKQIESLSSLNFMSSKTKKFLDPSETPEARLEQQRKANEVALKKENEFKETNRKNQEKFKRSEEAKKKQEEEKKRRQEEKKKERQEREEKEKNEIALSFKFPLERSNSGGSQELDPELEDDERYLSRRPQQQQQKQPTSSFSSFDVFSTTTPNANDDSAKTVDPKFDMNNNNYNINFDLKSTTTSSIFDGLEKDNNNNNSNTQDKEMISTQDSIIFTPSLSLGAIIDNDIDEKTNNTMNEIQKDNDKTITLENDKKEEQTPIDNKEEVKENEKVENEKINKDIVQENKPEIIKEDKDINKEKINENINNHKEILEQQITKNVNEAQLDNNNNKNKNNNDDSINNNDNDEIPSTQACQDEYQLYTEAIQGIYEPLSYEEYKKSRKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU35</accession>
    <name>A0A8J4PU35_9MYCE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_006082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000265">
        <property type="protein sequence ID" value="KAF2072604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PU35"/>
    <dbReference type="OrthoDB" id="21162at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="387"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="502"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="232"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="341"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="388"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2072604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="664" mass="73615" checksum="7FFB9D516743CEB5" modified="2022-05-25" version="1">MNNMNNMNGMNGMNMNMNNMYHPYSQPGTPRRGVVTPPIVNRLPDNLPPIESLNPDTLKDLSKTDLTAYLKAHNIPPAVSKPEMKKQMRTLIHSIKNKEELPASLFNPTQSSKTMADLASLSGMTVQQQQQQQLVQQQQQQPQQNGGMGIVNPMNINPMAATMGMGMGMGFNHLSNIVPPMVNINPMGFASPFGALNQSYPAPFFAPPAAAPPPPPPLLQLHAPPPPPPIVPQSIVTPTTTTPGSTAANTPASSTPAGPQSPPHSPRTANKDPADQHCAECSKKANRECTNKRCRGCCVKFMVERKGSSICHLHIKDESRRPRSKDQAGFPEAVQNLIQQAAAQQAGTNPPSSASSVHNSTSNTPVTSTPTLKGKAAAEIIPPTTPVLQSAQTPTSATQPAATTQQQQQQQQQQQQSQLQSSYGRRVQSYYHYHFIGVQASVAACSNPKRLAPHKDDKHSFFSCFKCDKVVSVNGSHLWKHIQTEHPQDFFKFIKEAYKHKVEDDDQDQQDSTNNNPDKEDIYYLTKQKASERFNLTMNWLQELFSPTPGPEVSESEFFKVRKQNILEFSEKLEDIVSRFELSKATLLDSLHNDSHQFKQFYDQLPNLKSEQELEDFIKQYENEKHVYIQPKPSLVTPYVSSSVVDHTGSSTSNSENTFKYLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PU66</accession>
    <name>A0A8J4PU66_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">BEACH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">CYY_005471</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000219">
        <property type="protein sequence ID" value="KAF2073220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="26681at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007040">
        <property type="term" value="P:lysosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016050">
        <property type="term" value="P:vesicle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06071">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01201">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000292">
        <property type="entry name" value="Lysosomal trafficking regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1540.10:FF:000002">
        <property type="entry name" value="WD repeat and FYVE domain containing 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1540.10">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036372">
        <property type="entry name" value="BEACH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050865">
        <property type="entry name" value="BEACH_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046851">
        <property type="entry name" value="NBCH_WD40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031570">
        <property type="entry name" value="NBEA/BDCP_DUF4704"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023362">
        <property type="entry name" value="PH-BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743">
        <property type="entry name" value="BEIGE/BEACH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743:SF86">
        <property type="entry name" value="LYSOSOMAL-TRAFFICKING REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02138">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15787">
        <property type="entry name" value="DUF4704"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20426">
        <property type="entry name" value="NBCH_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14844">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01026">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81837">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51783">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BEACH-type PH" evidence="7">
        <location>
            <begin position="3112"/>
            <end position="3246"/>
        </location>
    </feature>
    <feature type="domain" description="BEACH" evidence="6">
        <location>
            <begin position="3258"/>
            <end position="3549"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="3688"/>
            <end position="3722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="315"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="619"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1151"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1191"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1555"/>
            <end position="1611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1794"/>
            <end position="1813"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2122"/>
            <end position="2150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2169"/>
            <end position="2242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2359"/>
            <end position="2390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2627"/>
            <end position="2704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2992"/>
            <end position="3018"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="3155"/>
            <end position="3188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="34"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="315"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="335"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="357"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
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            <begin position="1154"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
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            <begin position="2125"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="5">
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
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            <begin position="2206"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="2359"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="2627"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="2681"/>
            <end position="2690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="2695"/>
            <end position="2704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="3173"/>
            <end position="3183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAF2073220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="3869" mass="431507" checksum="B13895CC9B824A73" modified="2022-05-25" version="1">MNFTPSSNGTGGGTSPPPTPMFYTPLSPSSKTGNNTGYSNNNNNNNMYSSNVNNSSSSSQQQQQQQLYNSNTLKVLWNTYTQTVVYQDSLFALIEFLKYFVQTYYQETSMGGGTGASGGGATASGVGGMGGNGANSGNSNAGSVANRNGLGSNSTTSSVLLFLSSGTLTDITRHLSKHLIEEIGYSCTAATPNNNNNNSAEYEIYQYLIGKSIVGEGPLLLSALDILSRQNPNGIPISFVKFLCVVLTQIFTLPSTMLYQDHSFKHHNHPHHHQQHSGHHHYHHQQQPFINFKSVFQPIDSFFYLSPLPSSLTDTFRDTSTPSNSNSNNNSNSNDIDDPTDDDNTTSILQGESFMDLLNSTTSGNSNSNSNSNSNSNSNSNSNTPNPLSLSSTSILKQSKPNIIKQQHQQQQQQQQQQQQQQQQLDISSVSILGYQILKIIGNLVKEKEFFYELLTTDCLSRLVNIFMIALPKDNQQNVTHKSISNDASFKVVYQELIQIFSSLMSQCCLSIDTITLVHNFKLVKILLNILDKLLSVLPLELIIQISQLLIYTIKSSSRLSKSMHEDFIKNNGYSILLKCFIHIADHGTLKHKSDFIQSSTNLLFIGVGITLNNNNNSNSSGGNSNSNSNLSSSTTLASNSSSNSATPTTTTIYTEIPNIKIFEIYLNVFSITNNEETKTEILRCIRGVFYTSILEMNLFDDNVEANSNSNNSGGNSNSNNINTTMIVFHQLEPFKILFSQFDCLSFSNRKMILDMMDVLLMKNRISILEIKFYCQLFKSQSPSTVLLVCQHLTDLLNKSKINSIVLGKECGMVQTLLEYLKVEPEKLELLSILESSPSEVQLCFSLIPSGKYFAGGSKGKYLILYMIVSKVLDLLLELFTPAPVIQTIFVDNQGLQALYSLLKNQYLIQPTLKVIACLAIGGLAIESKIIQGLIEVLQMNGGVSTLDSNILTMRKSILSTMCYIFHHNNIAKNSFKNAGGFIWSVSILDGISRCVNNLNADGEACQMSNSTSSLPHAATGATGKDIISNSPSLASSHEIFFFLKVLIDTLSAVMKNNPSNQDYFRKEIQFSTLSSTLKACGYMKGVYATSLCDSLLNMAVSGSWPPSCQDHSLEEGSLLSLYSPITSFFPTHFSLAKHFEIHYKEPNSADDAIQQSQSNSPSLASMSNTSPANIGSGGVIQSPIISSIKGTTTTTRSRSYTNESLFGRSSPSKASLMASPSIPYDPTSPVETSPSSALSTPVSLDLASEIKPFSWLTISKLPEKSSLMLATERYICCQSCRDSLMIENPEIFKLIIILMSSEEEISATESKSSCYILKELIFLSTASLANQKRLSMLLVDIVNHFRPLLLSNTTTINSSNSSNKNSTSVPSYSSNIKRKQETLKLKPLLLELIQSLVKHNLTLLEFRKYFELLKVQDFPLDLLHLLLNVISNRGNIPLYYAELSKHGLEYIDFLSWSDKLWPPTKGFGISFWFRYSLPCLNINKSPIYLLSKEGVYGNRSCEIQLILDNNNLHYRINYSNAPSEQYCFSEFKFEPDQFYHVVISHGTHLSTVGGTSTAGGTSTTGSGSGINTTSATATTTTANVGGSSSMSNLNSSQSNSDRSMSVSSSSSKKTPVKLYVNGCLRGQSLISYPKPQLGSFYVRFGGTNASSANQDYSTNNSWHLGNAYYFEEMPSDKEVFYLYLLGPDHFRGLKVDISAIDSILPNLDKTTKLHPLLIEHLLSPSTAPLLSLHEKILYIFTAKCLCVTTHKIANKTEAVTHSSAQAISASSASLTPQEFSLASSLSMSRKQSLSAHSMLMPPGSPSVMSSSSNSSFTSGSGFSTSRFSASQGGLLLESPMLSRESSVMSIASTMAMPPSPMKISQSLSPNIPGSVALGPLSSTLGLNNSLTPQTISLIQTGLPYPLPTGVISQLGVKDVIMNSGGVSVIIYLVAMSDDKDYQRSSLRLLQSILHNSQRNLKDMRDISGYQIISYLIRKRNWTLDDNLLSILFSFLGIQSNRTSIPHYIDGVVQDVMALKHLLLERTIWSRANISEQKKLFESLEKLVNALHDYHEFNIIKFRQAGAFETILKMCREQDLPLELLTTLISILNAIIHKPPKLKDDLHILLSYLLETMPKTKKYHNSNNGNNNNNNSLSSSMNSNSLNGLSAPNTGSLVGVNTNSLIPSIAPNSSTNSPLSSSPNRKSLRKSSIVPTLILEDKSDNSENNNSSNNNSNNNSSGSNVDNNNNTTKTKRRSSKFIDEDLPQTSEEIIRVSILDLLLDVLSKNDVSVIEEFHSLCSLETVFGLLINDSMATRIRFLKIIDMFLHSATISSQFQKIKGFHLLGHQLQSYKSSEHIFNILFCILFGKSTINPDANNNNSNSNNNNSGNNGINGNLNQSSDEEDEVSNYLNQNQNNNRSISMYFLYQDSNDMKYPGVIISILIILCNSSTKLQHMIIKIIHNIFLQNDQFKANLLENELVPRLIDILASNFQKRSNQTFQYNYNYNMNIRNDDHSGDSSNNNHHNNDSWIVEESILSLLKEIALYGAKSQDGGTILRDILVILHLNTKMDYDYICCLQRRVLSEVITFFNENKFSAVDSLVSSYEKICVLTINTLSYQEKNMNKPPTTSNTLKKNFKFNFTKSSNSNSSSGVGNSSKQHNNNNNNTSSIPISTSSSSLNNESTSDGGLDISNNDLSNEESSTTDSDSPITPPHHHHHHHKSKKPFIPIWIKEGHLFDQEQFIDLLIKVLSNTKIPQSNSTYRNLFSTQYSARSLLCKLIFILLTFTEYQSYQSLVLSKLVEPAVLNEILAEEDFILVLLHITIKFMNFPSAFPSSPLPSSPFSQSPILSSSNESFDVTKNCFKLWTTIIQASLSNTETMKRVFDISTISLSTLANTTDPRDLLIRYQNQYVENEYKKWEEKNNQSKKEWKLQYLEIQKNKQNIIIKNQDITKSTKKLSESLCQLKTEYQKTNLQYLVQNRESRRFFQNQWKLLVKKVTHDKAIWNSNVPLEAEEDESPNAVDSSSDESLLLNKDDSKNRMNKVDLSKENIHMVKWKLDPTEGPHRTRMRLKRIPPVILNSANNKNNDILKITTLVPDLNSPVVGKAELHLFNNINNQSSLDYSSFDHLQLGEKVDQNFKCSCISPFYQRDGELLIGDQNVYFLDESLTTAERQRSVNNSTSANTGGGGELSSNDKASISSIKPPHRGKHITWAYEDIIEIHKRRHLLKNSAIEIFLGSGAPHKTYLFAFNKTSDRDVVYDLIMSKPLPNRVDYASEVQGNILKMSITKKWQRGLISNFEYLMHLNTLAGRSFNDLTQYPIFPFILKDYESETLDLENPDTFRDLTKPMGAQDPSRLKKFVEKFNYLLETNETPYHYGSHYSNIGSVLHFLVRLQPFSSYFIEFQGGRFDVPDRAFHSISQTWKLSSSLSNSDVKELIPEFFYLPDFLVNSNKFNMGTKQDGVKVDDVVLPPWAKNDPRLFIKKHMEALECKFVSENLHHWIDLMFGYKQQGEAAVRAFNMFFPLTYEGAVDIDSIEDSLTRDATIAQIHSYGQTPKLLFTKPHPKKNWSKTSNLYQDSIYSKPEKLSSYLMFNQRSPIGSISIAGDSPIHLPPQRILFYPDNNKSISWGHWDQNLRINSIDTGKVLSVVEVFNDDIICCDITKNGRLFVTGGTAGTVKVWKRCNNDGTIMTRKERGDNLALWSTLYGHTNSILCITVSQEYSIIISGSKDGSCIIWDLNRLCYVNSLKHKNPVTSVQVSPTTNYISTFETSDNFNHGCLRLWNGNAQKISKKKLNDRVNCMIFTSGIEGLHTNILITGMESGLIILWNAWNLVKIRELTPAVNHPSPITALTISKDHNQLIVGDSVGNIVCFSVRTFDVYSAIGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUD0</accession>
    <name>A0A8J4PUD0_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sestrin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_005082</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sestrin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000193">
        <property type="protein sequence ID" value="KAF2073626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUD0"/>
    <dbReference type="OrthoDB" id="337464at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070728">
        <property type="term" value="F:L-leucine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016684">
        <property type="term" value="F:oxidoreductase activity, acting on peroxide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071233">
        <property type="term" value="P:cellular response to L-leucine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990253">
        <property type="term" value="P:cellular response to leucine starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016239">
        <property type="term" value="P:positive regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901031">
        <property type="term" value="P:regulation of response to reactive oxygen species"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006730">
        <property type="entry name" value="Sestrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12474">
        <property type="entry name" value="P53 REGULATED PA26 NUCLEAR PROTEIN SESTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12474:SF0">
        <property type="entry name" value="SESTRIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04636">
        <property type="entry name" value="PA26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="355"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="382"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2073626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="597" mass="67651" checksum="569C760CF114240F" modified="2022-05-25" version="1">MMSNKGQGIYGSSTASGNYHGSSEWIISSSTSPYHTNKRYSLDTPYGSDYSPPQSPSLSEPLSAHFLSLQTTDSSIRKSAIEHILSILTNPENEKLVKNYLNTIIVLATESPFDDITEAFSSFIKTIQDKYHIPKQRTTYYINDEQLPKLNTQDELTSKLFQDVFIQNGRVNHLTRILGWHPQYLERFLSAYNTIMKDPGPLPITWRNYIGILAAARYNCSYIVSLQEHEFLQNNGDPRWLQGIEFIPAKLKNLLKVIELMAHQPWLLPKLDIEYLVKGSDAWSIAELVQAMVLITTFLSLSGLVFCCGIAPEVGLSDSNGLGGSFSINDSDAEIEDTSASENTANLMELLKNRKSSATNDEDDDSEEHDRQQDFHNAGIESSSSNSTTSSSSLSSSTLLSSSSGEQGSTEKKSTTATVGTQTETHDFSRYKGNYNLSHTDFDVSSREYSIFSVQEYSWKEHGYELVSRYFPDAAPLLEEEFSFVYTMTYYEFNNNPDIDTLPFRRAVWYYVQRVKGMCHDDYNYQEVNMFLSISLKNFVKKAACFPDTIKRDDFSKLGYHLKPDEKAHLSLLAVCSHKQASLLYGLYTVMEYQNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUM7</accession>
    <name>A0A8J4PUM7_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WD40 repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">CYY_003503</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000110">
        <property type="protein sequence ID" value="KAF2075198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUM7"/>
    <dbReference type="OrthoDB" id="5591786at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050505">
        <property type="entry name" value="WDR55/POC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019:SF8">
        <property type="entry name" value="POC1 CENTRIOLAR PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="203"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="286"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="342"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="355"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAF2075198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="593" mass="67761" checksum="CE48AC51BC6D077F" modified="2022-05-25" version="1">MNDSDIEYSNSVNNTSMEQEQQDHVLVEPSPLLNFYFDNNKLDVVDDSPFRLVNLKATEFNTNSQIPNLHILPWKLNLMEVSLNNRLLFIGESHRLAVFDLDDIITPIYTVDLQNNGMTINQIRLGVLGGEEVLITVDEGGYIRILFVNDLDREYLRFYNAGISTWGIAMCPSKPLFAVSSNDFKINIWNLENEDPHASRRQLLEHKHNIPCIDFSPCGNFLVSVSIDKYVRVWDVETHTMLSSVVLSQWAWGCRFVDLTPNLDQIGESYLKKDNTHTIQWKGLSREDEFEEVEEANNVQIDNDQDDDSDSELDLDDNDLDDHDNNGGDQAMLDENHHYINDDDDDDDDEDLDEYQDHNDAQKEGLDPQQQKIDSDNSDTNNDDDDEEIEYNKSIIERHRAMIEKVLNLEGTRFKLINDGLTAEKKKNLTLPSNLVFSTYQNLYFSPQMMEDLNIVHNAIPTTFPIVQTQIDRIALLEVVPELSLVIAASQGPARCVSLYRIKRLPNTNKYNLVLDQVLEPPEGGPGLIIGLSVVKNYSPIPSKFSVSLYILYINGTFISYNIERLSLDELNHLPPSEIPNSKKMIFGLDISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PUU1</accession>
    <name>A0A8J4PUU1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FNIP repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">CYY_005593</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000227">
        <property type="protein sequence ID" value="KAF2073099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PUU1"/>
    <dbReference type="OrthoDB" id="10290201at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008615">
        <property type="entry name" value="FNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051251">
        <property type="entry name" value="STK_FNIP-Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32134:SF92">
        <property type="entry name" value="FNIP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05725">
        <property type="entry name" value="FNIP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAF2073099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="591" mass="67297" checksum="589EB863F5D046A7" modified="2022-05-25" version="1">MFITEIWRNLYIRNNIRNKLLENVTIVMTVRYLNENNKYLQYIDHRYTNICVALDSLDEYSESPYRRLINIVEIHLPTTTLTKDTFGTDNSIVHLRIQSCLAIESLPDNIERLQLRYLDRDIDDSLQLPRNLLELELSNFDRDIRPNSLPSCLKTLRLSFNFNRPLLPNALPESLTLLSLGRCFKQSIGAGVLPTSLKELTMGGSPITPITNQLATAIFPPSLESFTITHDRFTTVPRVPFMVTKLLFHAFIFTKQLQVNDIPSSVVYLDLSRYTYKLYPNVIPYGVETLIASSYNLSIDVNDLPDSITSLDMQTFNQSLVNLPRKLSRLTLDSFNRPIAPNTLVEPLKYCHLPNFQHNLPPLPSSITYLNIKRADLLDLSILSNSCAKELVINDLLSPVVFNSSNGNNDFKHLTTIELVSSKYKLKAFIIPCTVKNVILLNYDFDLDVNAIPISVSSLYYSSNLNTEPTLHFPNSITNLTMHVNFPIRVGLVPSSVTCLSFMYHQYRLPMNSIPPSIRKLVFKQNLELPLEPDEIPNTVEKLVLQGWITDSLSLHSIPRTVSVLKSNSFIKDIDSIIDSKSDIILSNIFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVA9</accession>
    <name>A0A8J4PVA9_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BD-FAE-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">CYY_005278</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">QSvi11</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000206">
        <property type="protein sequence ID" value="KAF2073399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVA9"/>
    <dbReference type="OrthoDB" id="6495301at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049492">
        <property type="entry name" value="BD-FAE-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF33">
        <property type="entry name" value="KYNURENINE FORMAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20434">
        <property type="entry name" value="BD-FAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BD-FAE-like" evidence="2">
        <location>
            <begin position="25"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAF2073399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="285" mass="32865" checksum="71965E0BC011571E" modified="2022-05-25" version="1">METQVREMLDLSYQDTSLGNNYYKLDLYLPAAVNSDKLPPLMVYVHGGLWMDRDKRDSKDIGFYFSDNGFAVAIVNYTLTKHSDNPVHHYPVHNRDLSKSLYWLLTNDSVNGLFDRENVHLIGHSCGAHMIALACMQWDQTMNGLSMDGIKSVVGLQGIYDVERFVNDFPNWTSEIGFIYNNSDPKSWESPQHIDPQSTTQTIRDAISNKIDWLLVHSPEDLWVNREQSIYFKQQMLTLWSCKKVIVDEDLHGAHFPVVETLTVKSDPTGDKLRNSLNQFYSKYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVH1</accession>
    <name>A0A8J4PVH1_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Exportin-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">CYY_004715</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">QSvi11</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the exportin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000172">
        <property type="protein sequence ID" value="KAF2073970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVH1"/>
    <dbReference type="OrthoDB" id="27218at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001255">
        <property type="entry name" value="Exportin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041123">
        <property type="entry name" value="CRM1_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041235">
        <property type="entry name" value="Exp1_repeat_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045065">
        <property type="entry name" value="XPO1/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014877">
        <property type="entry name" value="XPO1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040485">
        <property type="entry name" value="XPO1_repeat_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223">
        <property type="entry name" value="EXPORTIN 1/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223:SF2">
        <property type="entry name" value="EXPORTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08767">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18777">
        <property type="entry name" value="CRM1_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18784">
        <property type="entry name" value="CRM1_repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18787">
        <property type="entry name" value="CRM1_repeat_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01102">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="9">
        <location>
            <begin position="36"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1018"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1020"/>
            <end position="1032"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAF2073970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <sequence length="1053" mass="121444" checksum="48A8982893DE426A" modified="2022-05-25" version="1">MDKILNFNEPLDINCLDSVVRVLYDPKTTALDRQQAQKLLGQFQEHPDAWLRADSILEHSKETQAKFFALVILESLIKYRWKSLPREQCDGIKNYIVGLIIRLSSDAQTYARERVLLNKLDIIFVQILKQEWPHNWSSFIPEIVSSSKTNETLCENNMVILKTLSEEIFNFSEEQMTQAKIQSLKITFEREFSLINELCQYILENATKPSLIKSTLETLQRFLNWIPLHYIIEASSGVAQPSKLVQLLIFKYLPEPSFRTATLKCLAEIGSLNLSPQYDNFFVVIIDSFMIKIKQFKPDPKKIPEDYEDGDQGEHNFIQAVTIFLTSFFKSHLKLMENSRNIPYLTLAHELLVNISNVDELEIFKVCLEYWNFLSSNLYSDIGTYTSALMTSPPRLALYKSILSQVRIVLIEHMAKPEEVIVVEDESGNIVRELTKDTDSLTLYESMRSTLIFLTHLDSANTTEIMKNKLNLLVSGKEWSFAKLNTLCWAIGSISGAQSKSEEKSFLVNVIKDLLELCQVKKGKDNKAVVASDIMYIVGQYPRFLKDHWKFLKTVVNKLFEFMHEPHPGVQDMACDTFLKISKQCKRMFVIVQVEESQPFINELLTNLPVTIADLETNQIHIFYEAVGFMISSTPDPNLREKLITKFMELPNQSWVQIMSKASHNPETLYNLDVIRDILNLIKTNNRAATSLGNFYMSQISKIYLDLLNIYRAYSDYITKNPQIHTHTIGLRSRSVKKETLKLLETFIEKSQEQSMIYTNFIPPLLEAVLGDYKTNIPETRDPEVLSLMTVIITSLQQHVYPEVPKILEAVFETTLGMITKNFEDFPYHRINFFNLIRAINSHAFTVFYQLSSQQFKLLIDCVVWAFKHTERNISETGLHILKELIENVSKDQNVSNTFFKTYLVPLLNDILYILTDSFHKTGFPLQCDIMRMMFQVVENGMIKVPLSDQQQGSNSDYIKGVVADYLTASPNVSRPQIEAVVQRLFSLINVPNNDFKVAIRDFLIQLKEFQSSDNQELFSEERQAEKDETLKKAQAIPGMVRPNDIPEEMSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVK2</accession>
    <name>A0A8J4PVK2_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome P450 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">CYY_002916</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Polysphondylium violaceum</name>
        <dbReference type="NCBI Taxonomy" id="133409"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Amoebozoa</taxon>
            <taxon>Evosea</taxon>
            <taxon>Eumycetozoa</taxon>
            <taxon>Dictyostelia</taxon>
            <taxon>Dictyosteliales</taxon>
            <taxon>Dictyosteliaceae</taxon>
            <taxon>Polysphondylium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2020-01" db="EMBL/GenBank/DDBJ databases">
            <title>Development of genomics and gene disruption for Polysphondylium violaceum indicates a role for the polyketide synthase stlB in stalk morphogenesis.</title>
            <authorList>
                <person name="Narita B."/>
                <person name="Kawabe Y."/>
                <person name="Kin K."/>
                <person name="Saito T."/>
                <person name="Gibbs R."/>
                <person name="Kuspa A."/>
                <person name="Muzny D."/>
                <person name="Queller D."/>
                <person name="Richards S."/>
                <person name="Strassman J."/>
                <person name="Sucgang R."/>
                <person name="Worley K."/>
                <person name="Schaap P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">QSvi11</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AJWJ01000086">
        <property type="protein sequence ID" value="KAF2075783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8J4PVK2"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP000695562">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20617">
        <property type="entry name" value="CYP1_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF417">
        <property type="entry name" value="CYTOCHROME P450 508B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="432"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAF2075783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000695562"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="487" mass="56955" checksum="3AAF31BD515A4806" modified="2022-05-25" version="1">MIHYIFAFFIGFIFFDYLKKEIVNYQCKKLKGPSILPIIGNLYTIKDDTHKVLLKYHETYGPVYRVWMGDYFTIVINDLDLIKKVFVENYYNFLNHSDIPSIENISHNFNGLPFNLDKNEWKRRRIIIAGALSKIKLKNVYSAMTNINSQFLKTIKEFSDTKEPFEPHYYSDKLIVDINFKLFFNIDGGYDTTLDTFKKLADSFNNLVLELGTAKLSDYCFILQPFLRKYYHWFNKDRDYIDSFLKREFDLHFESFKPEYIENPRDLMDTMIGKIGENLDRPSLKKEVVEMLFNIFIGAIDTSAGIINWSFIYLANYQEIQEEVYLELKKIACDRPIELADRISTPFLNAFIKEVERIRPVIPLSVPRIGSEDIVIDGIKFPKNCQVIPNLYGIAFSEKYWDNPNEFNPSRFFTKSASSVNYSIFSYGPRACVGQRFAHDMLYLNIGNIIKSFKFSSFDGKPVDDTGVLGLSLKPKHCKLSVQERND</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-05-25" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8J4PVK3</accession>
    <name>A0A8J4PVK3_9MYCE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Putative rRNA methyltransferase</fullName>
            <ecNumber evidence="8">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">2'-O-ribose RNA methyltransferase SPB1 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">CYY
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